LOCUS       DATMJA010000001         4595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_473886, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000001 DATMJA010000000
VERSION     DATMJA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473886"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VNT12_00005"
     CDS             complement(<1..185)
                     /locus_tag="VNT12_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI44516.1"
                     /translation="MTEESDRRAQILEAAFEEFSAKGFKGATIKSIARTASLQSPALI
                     YWYFPDKEALFREVLGS"
     gene            316..462
                     /locus_tag="VNT12_00010"
     CDS             316..462
                     /locus_tag="VNT12_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44517.1"
                     /translation="MIFIVIQLINGALTAVLIGWVLLQIVGLWAMIDAYTTAKRNNER
                     YGFR"
     gene            473..664
                     /locus_tag="VNT12_00015"
     CDS             473..664
                     /locus_tag="VNT12_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44518.1"
                     /translation="MDERDLLTLESAVTAIEEAASAVAREVERDRLRETSLSRLSTIE
                     AELNRSQLALEKIIQEETA"
     gene            complement(778..1941)
                     /locus_tag="VNT12_00020"
     CDS             complement(778..1941)
                     /locus_tag="VNT12_00020"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HWI44519.1"
                     /translation="MELAGPNVLDVFIALFVVVVILRGARTGFLAGVFSLVGVVVGVS
                     AGSRIASSLMPEDGNSIYGAGITLGSILAFAVLGEVIARTIGGSIRNRLSSPASETLD
                     GFGGAVLGFALSLVLVWAVGVFALQSPPLAELHPAVRNSRILQALDERMPSGLLTRAV
                     ADLEPLPQIRGPEPEVSAPEGSIVRDPDVQAASASTLRVKGIACGYGVEGSGWVAGRN
                     LVVTNAHVVAGETETHVQVEGTGRRLPAKVVVFDEKNDIAVLRVNGLGLTPLHLAAPR
                     PGKDAAVIGFPENGPLDIEPARTGTTQRVISSDAYDSGPVERVVTSFRVYVRPGNSGG
                     PAVNEYGRVVSTIFASRTGSDNSGYGIPSRIVYRHLRMAADRTTPVSTEGCAN"
     gene            2127..3167
                     /gene="rlmN"
                     /locus_tag="VNT12_00025"
     CDS             2127..3167
                     /gene="rlmN"
                     /locus_tag="VNT12_00025"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWI44520.1"
                     /translation="MNVAELLPDVRAVLEERNEPSYRIRQVYKALTGSLVRDWREATN
                     LPRGLQVALSEEAPAVTLTLERVSQATDGTRKYLFHTRDGHAIETVMIPEKARRTVCI
                     STQVGCPMGCTFCATGLLGIKRNLKAREIAEQVFVCARDIAPERVTNVVVMGMGEPML
                     NYDATLVALKVLNDPDGFGLAARHIAVSTSGLVEKIRRFADEPEQFHLAISLHTPFED
                     ERREIMPVAARHTIPELMDAARYFVTRTRRKLFFEYTLLAGVNDQSRHVEALANLLDH
                     PLYHLNLLRFNWTDTGFSATSKAEAKRFLGYARELGLSATLRPGRGQDIDAACGQLAA
                     KDLSGRPAVHIA"
     gene            3171..3650
                     /locus_tag="VNT12_00030"
     CDS             3171..3650
                     /locus_tag="VNT12_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWI44521.1"
                     /translation="MKGGPAERLDPRAITVWRITGTLNLLPLLVGAGFAGWALSRSGG
                     VSLFFGVLPVLAILALAGVVVGVVPGLRWRRWRYEIRADEVDLQRGIFWISRTLVPLA
                     RIQHVDTRQGPLQRRFGLSTVVFYTAAGPNQIPELSTPVAERARDRIAELTREQDEL"
     gene            3640..>4595
                     /locus_tag="VNT12_00035"
     CDS             3640..>4595
                     /locus_tag="VNT12_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWI44522.1"
                     /translation="MSSEKRLHPVAMLVGAIETLRRWVSGLVFPGVVLLVSQGFSLWT
                     VTLFLLGLIVVAALAALWGFLSWRATTYTVTGNSFRLRQGVFQRNERTIPLEHVQSVD
                     TVQGIIQRAFGVVEVRIETAGGGASEPDASLAALDQGAAQTLRREIEGSKGERSEETA
                     GPTVLRRLSTRDLLLAGATSGQIGVAFSVLAIGFQFLDNFFPEDFVRRIVETLVPNWL
                     MVVLILVPSGLLLAWLLAIGGTVLAYSGFTLSRKGDFLYIKRGLLERREATIPLSRIQ
                     AVRISEGVLRQPFGLSSLRVESAGYGEDAGVSTTLFPLLPRN"
BASE COUNT          810 a         1463 c         1456 g          866 t
ORIGIN      
        1 ctggacccca gaacctccct gaaaagggcc tccttgtcag ggaagtacca gtagatgagc
       61 gccggagatt gcaaactcgc cgtccgcgca atgctcttga tcgtcgctcc cttgaagccc
      121 ttcgcagaga actcctcgaa agccgcctcc aggatctgcg cccgccggtc cgactcctca
      181 gtcacaatgc ctctttattg atcgtttaat caacgactat actcttgata gaacgttcag
      241 tcaagtctgg ctgccatcct cagcctcatc gttgcggggc tcggccagat ctacaacggc
      301 cagattggca aaggtgtgat cttcatcgtc atccagctca taaacggtgc gctgacagcc
      361 gttctgatcg gctgggtcct actccagatc gtgggccttt gggccatgat cgacgcctac
      421 acgaccgcga agcgcaacaa cgagcggtac ggcttcaggt aatatccgtc ccatggacga
      481 acgagatctg ctgacactag agagcgctgt cacggccata gaggaggcgg cctccgccgt
      541 cgcccgcgag gtcgagcgcg accggctgcg cgagacctcc ctctcccgcc tctcgacgat
      601 cgaagccgaa ctcaacagga gccagctcgc gctggagaag atcatccagg aagagacagc
      661 gtaaagcatg tcagcaagtc gcctcgaaca ggcgacttgt cagcatttca gctttcgcga
      721 gcgcgagagt cctggttaac tggctgaaaa ggggcggagc cgccgtgctg acatgctcta
      781 attcgcacat ccctcggtgc tgacgggggt ggtacggtcg gcggccatcc ggaggtgacg
      841 gtagactatg cgggagggga tcccatagcc tgagttgtcc gagccggtgc ggctggcgaa
      901 gatggtcgag acgacccgcc cgtactcgtt gacggcgggg ccgcccgagt tgcctggcct
      961 tacgtatacg cggaagctgg tgacgacgcg ctccaccggg ccactgtcgt aggcatcgct
     1021 ggagatgacg cgttgcgtcg tgcccgtgcg ggcaggctcg atgtcgagcg gaccgttttc
     1081 ggggaacccg atcacggcgg cgtctttgcc tggtctgggt gctgcaaggt gcagcggcgt
     1141 caggccaaga ccgtttacgc gcaggacggc tatgtcgttc ttctcgtcga agacgacgac
     1201 ctttgcaggc aggcgccgtc ccgtcccctc gacctgtaca tgcgtctccg tctccccggc
     1261 gacaacgtgg gcgttagtga ctaccaggtt ccggcccgcg acccagcccg agccctctac
     1321 gccgtagccg caggctatgc ctttcacacg cagtgtactc gcgctcgccg cctgcacgtc
     1381 gggatctctg acgatgcttc cttctggggc tgacacctct ggttcgggcc cccggatttg
     1441 aggcagcggt tccagatcgg cgacggcccg cgtgaggaga ccggagggca ttcgttcgtc
     1501 gagggcctgc aggatcctgg agttccgcac cgcagggtga agctccgcga ggggaggtga
     1561 ctggagggca aagacgccga cggcccacac cagcacgagg gagagcgcga accctagaac
     1621 cgccccgcca aatccgtcca gcgtctcgga agccgggctg gagagtcggt tcctgatcga
     1681 acctcctatc gtcctggcga tgacctcccc gagtacggcg aaggcgagga tgctcccgag
     1741 ggttataccc gcgccgtaga tagagttccc gtcctcgggc ataagggagg atgcgatcct
     1801 tgaccccgct gagacgccga cgaccacgcc aaccagcgag aagacgcctg cgagaaagcc
     1861 cgtcctcgcg ccgcgcagta tcacgacgac tacgaagagc gcgataaaca cgtccagaac
     1921 gttggggccg gcgagctcca ttgacagatt ctaccgccgg tgcaagcgct ttacctcccc
     1981 tggccgcccg tgctcaagct cgtcgccccg tccttacccg gtgagacggc atccaagagc
     2041 gttacgaaga ggtccaggag gagaaatcgc gttcgcgact tccctgcgat cttgtgtatg
     2101 ggcggccacg gtataatctc tcgaccatga atgtggcgga gctcttacct gatgtgcggg
     2161 cggtcctcga ggagaggaac gaaccatctt accgcatccg ccaggtctac aaagccctca
     2221 ccggttcgct cgtgcgcgac tggagagagg ccacgaacct gccccgagga ttgcaggtgg
     2281 cgctctccga ggaggctccg gcggttactt tgaccctgga gcgcgtctcc caggctaccg
     2341 acggtacgag aaagtatctg ttccacaccc gcgacggcca cgctatagag acggtcatga
     2401 tccccgagaa ggccaggcgc acagtatgca tctccacgca ggtcggatgc ccgatgggct
     2461 gcacgttctg tgcgacgggg ctgcttggga tcaagaggaa cctgaaggcg cgtgagatcg
     2521 ccgagcaggt cttcgtctgt gcccgcgaca tcgcaccgga gagggtgacc aacgtcgtgg
     2581 tgatgggtat gggcgagccg atgctcaact acgacgcgac gctcgtggcg ctgaaggtct
     2641 tgaacgaccc cgatggcttc ggattggcgg cgaggcacat tgcagtctcg accagcgggt
     2701 tggtggagaa gatccggcgt ttcgcggacg aacccgagca gttccacctg gcgatctcac
     2761 ttcacacccc cttcgaggac gagcgccgcg agatcatgcc agtagcagcc cgccatacca
     2821 tccccgagct gatggatgcc gcccgctatt tcgtgactcg gacgcgccgt aagctcttct
     2881 tcgagtacac gttgctcgcc ggcgtcaacg accagtcgcg ccacgtcgag gctctggcga
     2941 accttctcga tcacccgctg taccacctga acctgctccg gttcaactgg acggacaccg
     3001 gcttttcggc cacctcgaaa gcggaggcca agagattcct cgggtatgct agagagctag
     3061 gcctctcggc gacgcttcgt cctggccggg gccaggacat agatgccgcg tgcggccagc
     3121 tcgccgcgaa ggacctgagc gggcggcccg ccgttcacat cgcctgaccc gtgaagggcg
     3181 gacccgcaga gagactcgac ccgcgggcga tcactgtgtg gaggatcacg ggcactctca
     3241 acttgctgcc gctcctggtt ggagcaggtt tcgccggctg ggcgttgtca cgctccggtg
     3301 gcgtgtcgct cttcttcggg gttctacccg tgctcgcgat actcgcgcta gccggagtgg
     3361 tcgtcggtgt ggtgcctggg ttgcggtgga ggaggtggcg ttacgagatt cgggccgacg
     3421 aggtggacct gcagcgtggg atcttctgga tcagccgcac gctcgtgccg ctggcccgca
     3481 tccagcacgt ggacaccagg cagggaccac tgcagcgccg tttcgggctc tcgaccgtgg
     3541 tcttttacac cgctgccggg ccgaaccaga tccccgagct ctcgaccccc gtcgccgaaa
     3601 gagcgaggga ccgcatcgcc gagctgacca gggagcagga tgagctctga gaagcgtctc
     3661 caccccgtgg ctatgctcgt cggagctata gagacactcc ggcgctgggt cagcgggctc
     3721 gtctttcccg gcgtagtcct cctcgtgagt caggggttca gcctttggac ggtgacactc
     3781 ttcctgctcg gcctcatcgt cgtggcggcg ctggcggcgc tctggggttt cctctcttgg
     3841 cgggcgacga cctacacggt cacaggtaac tccttccggt tgcgccaggg cgtgttccag
     3901 aggaacgaac ggaccatacc gttggagcac gtacagtcgg tggacaccgt ccaggggatc
     3961 atccagcgcg ccttcggcgt tgtcgaggtg cggatcgaga cggctggcgg aggcgcgagc
     4021 gagcctgacg cctcgctcgc tgcgctggac cagggcgcgg cacagacgct gcggcgtgag
     4081 atcgaaggtt ctaagggtga gagatctgag gagactgcag gtccaacggt cctccgtcgg
     4141 ctctcgacgc gcgacttgct gctcgccggg gccacgagcg gccagatcgg ggtcgccttc
     4201 tcggtgctcg ccatcgggtt tcaattcctg gacaattttt tcccggagga tttcgtcagg
     4261 cgcattgtcg agaccctcgt gccgaactgg ctcatggtgg tcctgatcct ggtgccgtct
     4321 ggcttgctcc tggcctggct gcttgcgata ggcggaaccg tcctcgccta ctctggtttc
     4381 acgctctccc gcaaagggga cttcctgtat atcaagcgtg gtcttctgga acgccgcgag
     4441 gcgaccatac cgctctcccg catacaggcc gtccgcatct ccgagggtgt actgcgccaa
     4501 cccttcggcc tctcctccct gcgtgtggag agcgccggtt acggcgagga cgcaggtgtc
     4561 tcgaccacgc tctttcctct tttgccccgc aacga
//
LOCUS       DATMJA010000002         3537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_355595, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000002 DATMJA010000000
VERSION     DATMJA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355595"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VNT12_00040"
     CDS             <1..534
                     /locus_tag="VNT12_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWI44523.1"
                     /translation="EVIIGSGPRALLFRSAAGMRRMDPVDQLSLEWAGSYRHYHAAMM
                     RTLAVGGATAYQRHLFSTVREALEKMTEALAPGRPVGEVDDAHRRVLDAAGLEEHRFA
                     ACGYSLGTTFSPNWMDWPMLFSGNPVLAEPGMVFFLHCVVADSGRGVAMSLGRTCLVT
                     GAGREVLSARPLELIVV"
     gene            630..2084
                     /locus_tag="VNT12_00045"
     CDS             630..2084
                     /locus_tag="VNT12_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWI44524.1"
                     /translation="MAEAGAERRDRGRQEGHLQQGLKRRHMTMIAIGGVIGAGLFVGT
                     GPILNQAGPATILTYLLTGTLLILIMRMLGEMAVALPSVGSFSDYSRMALGNWAGFAV
                     GWLYWYFWAIVVGFEATVAASILGQYIPGVAPWLISLILILLLTATNLYSVGSYGEFE
                     FWFASIKVAAIIIFIALAALFVIGLLPGGGVGLSNLYAEGGFFPNGGLVMFSGVVTVI
                     FAFVGAEIVTIAASESNEPERGVARATNQVIYRILLFYVVSVFFLAAVVPWNTTFAEE
                     VIKSPFTIAFQKMGLPFAPTLMNLVVLTAVLSVLNSSLYTTSRMLFALTRHNDAPQFL
                     LNTTRRGVPMWAILAGTAFGYVSVVIYYFFPENVFTWLINASGAIALFAYLLIAISEL
                     VMRRRLEREAPERLQLKMWLYPWLTYLSIAAMVAVLIAMAVIPAQRPLLIASLVSLGV
                     ILIAYVIRRVAGSPERDPHEVIRERETEPDPQTE"
     gene            2092..3045
                     /gene="speB"
                     /locus_tag="VNT12_00050"
     CDS             2092..3045
                     /gene="speB"
                     /locus_tag="VNT12_00050"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWI44525.1"
                     /translation="MTRYESADSFQTPRFSGVRTFMRLPNVRDLENADAAIVGAPFDT
                     GATFRVGARFGPEGIRSASHLLRRYNPSLDVAIFDYLSVIDYGDVPVVPGYIEESYRR
                     IEEGLKHIHRAGVVPIVLGGDHSIALPELRAAAAVHGPLALVQFDSHPDTWDAYFGEK
                     HTHGTPFRRAVEEGLIDTSRSVQVGMRGSIYDRGDWDDAREMGFDLVPTDEVRELGIP
                     SVIDRIRGRTGDAKVYVSFDVDFVDPAYAPGTGTPEIGGFTSREAQEFVQGLTGLEIV
                     GCDVVEVYPAYDHAQITALLAANLAHEFLSLLAFSKKTGSM"
     gene            3139..>3537
                     /locus_tag="VNT12_00055"
     CDS             3139..>3537
                     /locus_tag="VNT12_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI44526.1"
                     /translation="MLESTRIKTEIPGPKSRALMERRHNAVSSGVGIATPMFAKEAKG
                     ALLTDVDGNTFIDFGGGIGVMNVGHTDSRVVEAVKEQAERLTHSCFYVTEYEGYLQLA
                     EKLNALLPGGTEKRSFFVNSGAEAVENAAKI"
BASE COUNT          619 a         1100 c         1146 g          672 t
ORIGIN      
        1 gaggtgatca tcggctccgg gccacgcgcc ctcctgttcc gctcagctgc cggcatgcgc
       61 cgcatggacc ctgtcgacca gttgtctctg gagtgggcag gctcctacag gcactatcac
      121 gcggccatga tgcgtaccct ggccgtgggc ggggccacag cctaccagcg acatctcttc
      181 tccaccgttc gcgaggcgct cgagaagatg accgaggccc tggcaccagg acgtccggtc
      241 ggcgaggtcg acgatgccca ccgccgcgta ctggatgcgg cagggctcga agagcatcgc
      301 ttcgccgcgt gcgggtactc tctgggcacg accttcagcc ccaactggat ggactggccg
      361 atgctgttct cggggaaccc cgtgctggcc gagccgggga tggtcttttt cttgcattgc
      421 gtggtggccg actccgggcg cggggtcgcc atgagcctgg gccgcacctg cctggtgacc
      481 ggcgcaggaa gggaggttct ctccgctcgg cctctggagt tgatcgtcgt ctgaggtgga
      541 cttgggatgg ccgatgcctg aatgttgtaa aagagcgtgc ataaagtgta agctgcgcgc
      601 ggagcgaaca aacataggga aggggaaaaa tggctgaagc gggcgcagag cggcgcgaca
      661 ggggtcgcca ggagggccac ctgcaacaag gtctcaagag gcggcacatg accatgatcg
      721 ccatcggcgg ggtcatcgga gccggtctct tcgtggggac gggaccgatc ctcaaccagg
      781 ctggtcctgc gaccatcctc acctatctgc tgacggggac cctccttatt ctaatcatgc
      841 ggatgctcgg ggagatggcc gtggctctgc cctcggtcgg ctcgttctcc gactacagcc
      901 ggatggctct gggcaactgg gccggttttg ccgtcgggtg gctctactgg tacttctggg
      961 cgatcgtggt cggcttcgag gccaccgtgg cggcgagcat cctggggcag tacataccgg
     1021 gtgtagcgcc ctggctcata tctctgatcc tgatcctgct gctcaccgcg accaacctct
     1081 attccgtcgg ctcttacggc gagttcgagt tctggttcgc cagcataaag gtggcggcga
     1141 tcatcatttt catcgccctg gccgcgctgt tcgtgatcgg attgctaccg ggcgggggtg
     1201 tcgggctctc gaacctctac gctgagggcg gcttcttccc caacggtggt ctggtgatgt
     1261 ttagcggtgt cgtcacggtg atcttcgcgt tcgtcggggc ggagatcgtc accatcgcgg
     1321 cctcagagtc caacgagccc gagcggggcg tggcccgcgc gacaaaccag gtcatctaca
     1381 ggatcctgct cttctacgtg gtctcggtct tcttcctcgc ggccgtcgtg ccgtggaaca
     1441 ccacgttcgc cgaagaggtc atcaagagcc cgttcaccat cgcgttccag aagatggggc
     1501 taccgtttgc gcccacgctg atgaacttag tggtcctgac cgccgtcttg agcgtcctca
     1561 actcaagcct ctacacgacc tcgcggatgc tcttcgccct cacgaggcac aacgacgcgc
     1621 cgcaattttt gctcaacacc acccgccgcg gcgtgccgat gtgggcgatc ctcgccggga
     1681 ccgccttcgg ctacgtctcc gtggtgatct actacttctt ccctgagaac gtattcacgt
     1741 ggctgatcaa cgcctcgggc gccatcgcgc tcttcgccta cctgctgatc gccatctcgg
     1801 agctcgtgat gcgccgccgc ctggagcgcg aggcgcccga gaggctccag ttgaagatgt
     1861 ggctctaccc gtggcttacg tacctgtcga tagccgccat ggtggcggtg ctcatcgcga
     1921 tggctgtgat cccggcccag cgtcccctcc tgatagcgag cctggtcagc ctcggcgtga
     1981 ttctgatagc ctacgtgatc aggcgcgtgg ccggctcacc ggagagggat cctcacgagg
     2041 tgatcaggga acgggagacg gagcccgatc cccagaccga atgagagaca attgacccgc
     2101 tacgagtcgg ccgactcgtt ccagacaccg cgcttctcgg gcgtgcgcac gttcatgcgc
     2161 ctgcccaacg tcagggacct ggagaacgcg gacgccgcga tagtaggcgc gcctttcgat
     2221 acgggcgcca cgttcagggt cggggcccgc ttcggccccg aggggatacg cagcgcctcc
     2281 cacctcctga ggcgctacaa cccgagcctg gacgtggcga tcttcgacta tctctcggtc
     2341 atagactacg gcgacgttcc cgtcgtcccc ggctacatag aggagagcta caggcgtata
     2401 gaggaaggac tcaagcacat acaccgcgct ggtgtcgttc ccattgtcct gggcggcgac
     2461 cactctatag ccttgccgga acttcgggcg gctgcggcgg tacacggacc gctcgccctg
     2521 gtgcagttcg actcccaccc cgatacctgg gatgcctact tcggcgagaa gcacacgcac
     2581 ggcacgccgt tcaggcgtgc tgtcgaagag ggactcatcg atacctcgcg ttccgtccag
     2641 gtcgggatga ggggttcgat ctacgacagg ggggattggg atgacgcgcg cgagatgggc
     2701 ttcgacctcg tcccgacaga cgaggtccgc gagctcggga taccgagcgt gattgatcgt
     2761 atccgcgggc ggaccggcga cgcgaaggta tacgtctcct tcgacgtgga cttcgtcgac
     2821 cccgcatacg cccctgggac ggggacgccg gagatcgggg gcttcacaag ccgcgaggcg
     2881 caggagttcg tgcagggtct caccggcctc gagatcgtcg gctgcgacgt ggtggaggtc
     2941 tatcctgcct acgaccacgc ccagatcacg gccctcctcg ccgccaactt ggcacacgag
     3001 ttcctcagcc tgctcgcgtt ctcgaagaag acgggatcga tgtaaacaat gcgctcgact
     3061 atctcaccgg aggtatcact cgggagctga cggggagtat actggaacga attagcccga
     3121 caggaagagg tggcgactat gctcgagtcc acgaggatca agaccgagat accgggtccg
     3181 aagagccggg ctttgatgga gcggcggcac aacgccgtct cgtcgggggt tgggatcgcg
     3241 acaccgatgt ttgccaaaga agcgaaaggc gcccttctca ccgatgtcga cggcaacacg
     3301 ttcatagact tcggcggcgg catcggggtg atgaacgtag ggcacacgga ctctcgggtg
     3361 gtcgaggcgg taaaggagca ggccgagagg ctcacccaca gttgcttcta cgtcaccgag
     3421 tacgagggct atctgcagct ggccgagaag ctgaatgcgc tgctaccagg cggcacggag
     3481 aagcgctcgt tcttcgtaaa ctcaggggcc gaggcggtcg agaacgcggc caagata
//
LOCUS       DATMJA010000003         4198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_296328, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000003 DATMJA010000000
VERSION     DATMJA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296328"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /gene="ruvC"
                     /locus_tag="VNT12_00060"
     CDS             <1..488
                     /gene="ruvC"
                     /locus_tag="VNT12_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWI44527.1"
                     /translation="GIDPGTATMGWGVIRQEGNRLRYMQHGAITTPPDWEMPRRLGRL
                     FDGVTELVRGYMPGTIAVEELFFNTNVTTAITVGQARGVALLAAYKAGIEVAEYTPLQ
                     VKQAITSYGRADKRQMQEMVKALLNLPAIPRPDDAADGLAIAICHAFSSSLPGKVGVG
                     K"
     gene            513..1103
                     /gene="ruvA"
                     /locus_tag="VNT12_00065"
     CDS             513..1103
                     /gene="ruvA"
                     /locus_tag="VNT12_00065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWI44528.1"
                     /translation="MIYRLRGTLVEKDTEGVVIDVGGVGYRASASLATLRALPSLGEE
                     CVIHTRMVVREDAMLLFGFAEREERAAFDALTAVSKVGPKLALSILSSMSPPEISETV
                     ARGDVIKFASVPGLGKKTAERLVLELKGKSLAAFGPEPVMTGGGGGGPYVEARDALTA
                     LGYRLEEAEKALNGVPPQDSVEKYIKEALRRIGSRR"
     gene            1100..2194
                     /gene="ruvB"
                     /locus_tag="VNT12_00070"
     CDS             1100..2194
                     /gene="ruvB"
                     /locus_tag="VNT12_00070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWI44529.1"
                     /translation="MSAEEMDDFTVRGERETEESPEDITGAAGFRDFDEHPLDPEAHA
                     GDDEPSLRPSSFDEFVGQEALKQNLKIFVQAAKQRDEPLDHILLDGPPGLGKTTLCRI
                     LAEEMGVGLRPTSGPTLERAGDMAAIVTALEERDFLFIDEIHRLNRQIEEVLYPAMED
                     FAMDIVLGQGPSARTIRMDLPHFTLVGATTRKGLLTKPLLDRFGVAERLEYYGPEDLE
                     KIVVRNAKILGVPITEGGAKQLARRSRGTPRIVNRLLKRVRDYAQVVGDGKIDEDTAN
                     AALDMQGVDHLGLDRADRDYLGLVIDKFDGGPVGIGTISVALGEARDTVEDVYEPYLL
                     QSGLIQRTPRGRIATQRAYGHLGVPVPNNG"
     gene            2227..2445
                     /locus_tag="VNT12_00075"
     CDS             2227..2445
                     /locus_tag="VNT12_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44530.1"
                     /translation="MADTDSMLRYDVEPVNPVEAEVPGEEDLSFAVFSLAHAACGDAV
                     LEIRIGERMLLEWCTGCAVMEVFGSCET"
     gene            2727..3812
                     /locus_tag="VNT12_00080"
     CDS             2727..3812
                     /locus_tag="VNT12_00080"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HWI44531.1"
                     /translation="MLVARGVEERFDAFRFGLKRRLGLLDPFEILPYRGHGTTRELFL
                     RGRVLEEAGITRAGRDDAVWKNILNMARRFASDEVADARVRASFEGLQVETTADVEGF
                     FEVRFQLAEPLDGPGGWHQVGLELLSPPSPGGGRVRSKGHVLVPYGARFGVISDLDDT
                     VVRSSATNVLKMAWIVVRNNAHTRLPFEGIAAFYRALQLGPDSKSSNPVFYVSSSPWN
                     IYDMLVDFLNVHGVPHGPLFLKDWSLSVLGKHRDYKIGVIRRLLGTYEDLPFVLIGDS
                     GEEDPEIYLQAVQEHQGRIKAIYIRDVASVERDAEVRAMADEARKLGTEMVAVSETTA
                     AAEHAASIGLIAPDAIAAVRAESAASF"
     gene            complement(3823..>4198)
                     /locus_tag="VNT12_00085"
     CDS             complement(3823..>4198)
                     /locus_tag="VNT12_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI44532.1"
                     /translation="LPPGEMIPPHTHAREDECSFVLEGEMLCYVAGEVVLAQKGSYVI
                     KPRSVPHAFYNTGAETVRIMEILTPGSAFEGYFDEYEEIVSRQLSDKEHRRARVDLGE
                     RYRITWHDERIPEVEASFGIGS"
BASE COUNT          794 a         1205 c         1416 g          783 t
ORIGIN      
        1 taggcataga ccctggcacg gcgacgatgg ggtggggtgt gattcggcag gagggaaacc
       61 ggttgcgcta catgcagcac ggtgccatca ccacgccgcc tgactgggag atgcctcgca
      121 ggctcgggcg gctcttcgac ggcgtaaccg aactggtgcg aggttacatg cccggcacca
      181 tcgctgtcga ggagctcttc ttcaacacca acgtgaccac ggcgatcacg gtcgggcagg
      241 cgaggggtgt cgcgctacta gccgcttaca aggccgggat cgaggtggcc gagtacacgc
      301 ccttgcaggt caaacaggcg atcacatcgt acggaagggc cgacaagcgc cagatgcaag
      361 agatggtgaa agccctcctg aaccttccag ccatacctcg gccagacgac gcggccgacg
      421 gcttggctat agccatctgc cacgccttct ccagcagctt gccaggcaag gtcggggtgg
      481 ggaaatagag gatcttgcgt gataattagc ccatgatcta caggttgcgc ggtactctgg
      541 tcgagaaaga tacggaaggt gtagtaatcg atgttggagg cgttggctac agggcctccg
      601 cttcactggc gacgttgcgt gcgttaccct cgctgggcga ggagtgcgtc atccacacca
      661 ggatggtggt gcgcgaggat gcgatgctcc ttttcgggtt tgcggagcgt gaggagcggg
      721 ccgcgttcga cgcgctaaca gcggttagca aggtcgggcc gaagctggcc ctctccatac
      781 tgtcttcgat gtcgcccccg gagatctccg agacggtggc caggggggat gtgatcaagt
      841 tcgcctccgt gccagggctc gggaagaaga cggcggaacg gctcgtacta gagctcaagg
      901 gcaagagcct cgccgccttc ggcccagagc cggtcatgac tggtggcggt gggggtggtc
      961 cttacgtaga ggcgcgggat gcactaacgg cgctcgggta caggctcgag gaggccgaga
     1021 aggccctcaa tggcgtgccc ccgcaggatt ccgtggagaa gtacataaag gaggcgttac
     1081 ggcggatagg gagcaggcgt tgagcgcgga ggagatggac gacttcaccg tacggggaga
     1141 gagagagact gaggagtcgc ctgaggacat tacaggggct gcgggtttca gagacttcga
     1201 tgagcatccg ctcgaccccg aggcccacgc cggggacgac gagccctcgc tcaggccgag
     1261 ttccttcgac gagttcgtcg ggcaggaggc cctcaagcag aacctcaaga tcttcgtaca
     1321 ggctgccaag caacgcgatg agcctcttga ccacatactg ctcgacggcc ctcctggact
     1381 cggcaagact acgctgtgcc gcatcctcgc cgaggagatg ggggtcgggc tcaggccaac
     1441 gagcgggcca acactcgagc gggccgggga catggcggcg atcgtgaccg cgctggaaga
     1501 gcgtgacttc ctttttatag acgagatcca tcgccttaac aggcagatcg aggaggtcct
     1561 ctaccctgcg atggaggact tcgcgatgga catcgtcctt ggacaggggc cgtcggcccg
     1621 caccatcaga atggacctgc cgcatttcac gttggtcggc gcgaccacta ggaagggcct
     1681 gctcacaaag cctctgctcg atcgcttcgg agtggctgag aggctcgaat actacgggcc
     1741 cgaggacctg gagaagatcg tggtccgcaa cgcgaagata ctcggcgtcc ctatcacgga
     1801 aggaggcgcc aagcagctcg cgcgacgcag ccgcgggacg ccccgcatcg tcaatcggct
     1861 cctcaagcgg gtgcgcgact acgcccaggt cgtcggcgac gggaagatag acgaggatac
     1921 ggcaaacgcg gcgctcgaca tgcagggcgt cgaccacctc ggcctcgaca gggccgaccg
     1981 ggattacctc gggctcgtca ttgacaagtt cgacggcggg cctgtcggca ttggtacgat
     2041 ctccgtagcc ctcggtgagg cgcgcgatac cgtggaggat gtctacgagc catacctgct
     2101 gcagagcggc ttgatccaac gcaccccccg gggccggatc gccacccaga gggcctacgg
     2161 gcacctcggt gttcccgttc cgaacaacgg ttagtcaccg gccggtcgct aaggtcaagc
     2221 cgtacagtgg ccgatactga tagcatgctg cgctacgacg tggaaccggt caaccctgtg
     2281 gaggccgagg ttccaggtga agaagacctc tctttcgctg tttttagctt ggcgcatgcc
     2341 gcatgcgggg acgcagtgct ggagatccgt atcggcgagc gcatgctgct cgagtggtgc
     2401 accggatgcg ccgtcatgga ggttttcggg tcgtgcgaga cgtgacgcca gaggcgtcac
     2461 gtctcgcact ccggcatttc acagctaggc agctttttgt tttcgctgag agccggtctt
     2521 gtatagccga atgcgcaatt ctcggtaggt cgataccact gcgaatcgct gtagtactcg
     2581 aaaccttgtg tagtgtctac tcgccgtgtt agtttcggta ctacagggta ggttgatcca
     2641 ggaagagcga gaggcaagat ggtcgatcgg ggttcttccg agctagtcgg aatggaaacg
     2701 cgttggccga gttgaggcgg agggcgttgt tggtcgcacg gggggtcgag gaacgcttcg
     2761 acgcctttag gttcgggctg aagcggcgcc tcgggctgct ggatccgttc gagatcctac
     2821 cttaccgggg ccacggcacg accagggagc tgttcctgag gggccgcgta ctagaggagg
     2881 ctggcatcac ccgcgctggt cgggacgatg cggtatggaa gaacatactc aatatggctc
     2941 ggcgcttcgc gagcgatgag gtggccgacg cgcgggtgag ggcttctttc gagggtttgc
     3001 aagtcgaaac caccgctgac gtggagggct tcttcgaggt gcgcttccag cttgcggagc
     3061 cgctcgacgg tcctggcggg tggcaccagg tcgggttgga gctcctctca cctccgtcgc
     3121 cgggtggagg aagggtaaga tcgaagggac acgtgctcgt gccgtacgga gcccgtttcg
     3181 gtgtgatcag tgacctcgac gatacggtgg tgcgttccag cgcgacgaac gttctgaaga
     3241 tggcctggat agtggtacgc aacaacgccc acacgcggct acctttcgag ggcatcgccg
     3301 ctttttacag ggcgttgcaa ctcggccctg acagcaagtc ttccaacccg gtcttctacg
     3361 tgtctagcag tccctggaac atctacgata tgctcgttga cttcttgaac gtgcacggcg
     3421 ttccccatgg accgctgttt ctcaaggact ggagcctgag cgttctcggg aagcacagag
     3481 actacaagat aggggtgatc cggcgcctcc tgggcaccta cgaggacctg cctttcgtcc
     3541 tgatcgggga ctcgggggag gaggacccgg agatctattt gcaggccgtg caggagcacc
     3601 agggacgcat caaggccatt tacatcaggg acgtcgcttc ggtggagcgt gacgccgagg
     3661 tcagagcgat ggccgacgag gcgcgcaagc tcgggacgga gatggtggcg gtttccgaga
     3721 ccaccgcggc ggccgagcac gcggcctcca taggactcat agcgccggac gcgatagccg
     3781 cggtccgcgc cgaaagcgct gccagcttct agcgcactcc cgtcaagaac cgataccgaa
     3841 gctggcttct acctcgggga tgcgttcgtc gtgccaggtt atcctgtagc gctcgccgag
     3901 atcgacccgg gctcgcctgt gctccttgtc actaagctgc cgcgagacga tctcctcgta
     3961 ctcgtcgaag tagccttcga acgcgctgcc cggcgtgagg atctccatga tccgtacggt
     4021 ctctgcgccc gtgttgtaga aggcgtgggg tacgctccgc ggtttgatga cgtaggagcc
     4081 tttctgggct agcacgacct cgccggctac gtagcacagc atctcgccct ccaacacgaa
     4141 cgagcactca tcttcgcggg cgtgggtatg cgggggtatc atttcgcctg gcgggagg
//
LOCUS       DATMJA010000004         3085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_770263, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000004 DATMJA010000000
VERSION     DATMJA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770263"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VNT12_00090"
     CDS             complement(<1..222)
                     /locus_tag="VNT12_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44533.1"
                     /translation="MSFAIVGFAHREVVWEGYEADPRRADTVLHCQGNGGDAPFFYRV
                     ADQPDGPVAQGSRRREKHDVSPVFYEVAGD"
     gene            441..1133
                     /locus_tag="VNT12_00095"
     CDS             441..1133
                     /locus_tag="VNT12_00095"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HWI44534.1"
                     /translation="MALLVVLFIAFFVVVFLFLLPILFSLQAVGNLFVYPRQLRAIFG
                     NKILRRNHALEHATIVVMMEWEPGRKLNGFSTDDGFFVQGVRSISEVESAAKEAIRRL
                     QNGEKRLAIHRNCGTTIVAANLLAAVFFLVTLGFFLYLGVGNLYLMILGSVVLAFALR
                     IPLSLILQRFVTTDADLTNAEVGWVEPAQPGDLKSGIFGLLLAASTVRVRIFHTDPDA
                     VEILRDDGAIVR"
     gene            complement(1267..2094)
                     /locus_tag="VNT12_00100"
     CDS             complement(1267..2094)
                     /locus_tag="VNT12_00100"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HWI44535.1"
                     /translation="MRIYGLDFTSAPGRRKPLIVLCCALEGDSLRVEDSETLTDFGGF
                     EDFLQRRGPWVCGMDFPFGQPRSLVAALGWPESWEAYVGEVGALPKEAFEDKIRADMD
                     KRPAGRKWRYRLADRRSGSSSAMMLFRVPVGKMFYQGAPRLLASGVRVEPCRRNGDER
                     VAVEAYPAVVARRFLGRTAYKRDGVPDTPERRFARETLLAGLESAVLREVYGFAVEMD
                     ARWRDEFVQDPSADALDSLLCAVQAAWAYEKRDEGYGIPTECDLDEGWILDPALLDQ"
     gene            complement(2116..>3085)
                     /locus_tag="VNT12_00105"
     CDS             complement(2116..>3085)
                     /locus_tag="VNT12_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWI44536.1"
                     /translation="KKAESKGNEVEVPVAENLGLVDFLKLTYKEIDEDHVMAFAGNLT
                     YKGLFAIFPFFTFLLSLLGLFNATDLVNIMVDKLSGIAPQSATKFVEGQLLSITQSQA
                     ESAFTFGAIISILLALWGVSGAFRSVMEAMNVMYAVEEDRPFWKVYGLSIFMSLAVIV
                     LLVGALVLVIFGESIGVRVADLVGLGSVFATLWSIVQWPVVALVVLFAFALIYYFAPA
                     AKQRFRWISPGSILAFVFWLIFSLLFSLYASTAGSYNETYGSLAGVIILMLYVYYSAL
                     IMLVGAEINQVIEWHIPGGKNEGEKIPEDDRRPDVHRLNRENGEAR"
BASE COUNT          624 a          964 c          898 g          599 t
ORIGIN      
        1 gtcgccggca acctcataga agaccgggct gacgtcgtgc ttctcacgcc ttcgtgaccc
       61 ttgtgccacc ggtccgtcgg gctggtctgc aacgcgatag aagaacgggg catccccacc
      121 gttaccctgg caatggagca cggtgtcagc gcgccgcggg tcggcttcgt acccttccca
      181 tacaacctcc cgatgggcaa accccacgat cgcgaaggac acaagcgcat agcactcgcc
      241 gcactagccc tactctacga tctggagcag cccggcgaag tcagcatttc agctagtcag
      301 cattctcgct cgcgagacct agctgacaag gcgacgcagc cgccgtgctg aaattctgaa
      361 aagcggaggc gaaagccgga tcgtgctgac atgctgagat ataatcgacg tacatattga
      421 cctagtgcga aggtgagacc gtggccctgt tggtagtgtt gttcatagcg tttttcgtgg
      481 tggtgttcct cttcctgctg ccgatactgt tctcgctgca ggctgtggga aaccttttcg
      541 tctatccgcg gcagctaagg gcgatcttcg ggaacaagat cctgcgccgc aaccacgccc
      601 tggagcacgc gaccatagtg gtgatgatgg agtgggagcc aggccgcaag ctgaacggat
      661 tctccaccga cgacgggttc ttcgtgcagg gcgtacgctc catttccgag gtcgagagtg
      721 ctgccaaaga ggccatacgc aggctgcaga acggggagaa gaggctcgcc atccaccgca
      781 actgcggcac gaccatcgtg gccgcgaacc tgctagccgc ggtcttcttc ctggtcaccc
      841 tcgggttttt tctctatctt ggcgtgggta atctctacct catgatcctt ggcagcgtcg
      901 tcctcgcttt cgccctgcgg atccctttga gcctcatcct gcagcgattc gtcaccaccg
      961 atgcggacct gacgaacgcg gaagtcggtt gggtcgagcc ggcgcagccg ggcgacctaa
     1021 agagcggcat cttcgggctc ctgctcgccg cgtctaccgt gcgggtgcgc atcttccaca
     1081 ccgaccctga cgcggtcgag atcctccgcg acgacggtgc catagttcgt tagaaagtat
     1141 caggctacag gcataagcat tcgcaccgga tagtcctctt gctgagcagc acatttgcac
     1201 actgcggtgt catggccctt acctgatatc tgtaacctgt agccgcgatc tgcgaagcag
     1261 gctgcttcat tggtccagca atgctgggtc gaggatccaa ccctcgtcga gatcgcactc
     1321 cgtaggtatg ccgtatcctt cgtctctctt ttcgtaggcc cacgccgcct ggaccgcgca
     1381 caggagcgag tcgagggcgt cggcggaagg gtcctgtacg aactcatccc tccagcgagc
     1441 atccatctcc accgcaaatc catagacctc cctcagtacg gctgactcca ggccggcgag
     1501 caacgtctcc ctggcgaatc tcctctcggg tgtgtcgggc acgccgtctc tcttgtaagc
     1561 tgtgcggccc agaaagcggc gggcgacgac ggcagggtag gcctcgacgg ccacgcgttc
     1621 gtccccattc cgtctgcacg gctcgacacg gacgcccgat gctaggaggc gcggcgcccc
     1681 ctggtagaac attttgccta cggggaccct gaagagcatc atcgcgctgc tggatccgct
     1741 cctgcggtcg gcgagccggt aacgccactt gcggcctgcc ggccttttgt ccatgtcggc
     1801 gcggatcttg tcctcgaacg cctctttcgg gagcgcaccg acttcgccaa cataggcttc
     1861 ccaactctcc ggccacccga gggcggctac caaggatctc ggttgaccga acgggaagtc
     1921 catcccgcac acccagggcc cccgtctctg caagaagtct tcgaagcccc cgaagtctgt
     1981 cagcgtctcc gagtcctcga cacgtaagga gtctccttcc agggcgcagc agaggacgat
     2041 gaggggtttc cggcggccag gggcgctcgt gaagtcgagg ccatagatcc tcacgcgggt
     2101 tcgttcaagg cggacctacc gggcctcgcc gttttccctg ttcagccgat ggacgtctgg
     2161 tctgcggtcg tcttcaggga tcttctctcc ctcgttcttg ccgcccggga tgtgccattc
     2221 gatcacctga tttatctcgg cccccactag catgatgagc gccgagtagt agacgtagag
     2281 catcaggatg atcacacccg caagtgagcc gtacgtctcg ttgtaggagc cggccgtgct
     2341 cgcatacaac gagaacagca gcgagaagat aagccagaag acaaaggcga ggatagagcc
     2401 ggggctgatc caacggaacc tctgtttggc ggcgggggcg aaatagtaga tcaaggcgaa
     2461 ggcgaaaagg acgaccaggg ccacgacggg ccactgcacg atgctccaga gcgtcgcgaa
     2521 gactgaccct agaccgacaa ggtcggccac cctcacgccg atagactcac cgaagattac
     2581 aagcacgagc gcgccgacca gaagaacgat caccgcgagc gacatgaaga tcgagagccc
     2641 gtacaccttc cagaaaggcc tgtcttcctc gaccgcgtac atgacgttca tagcctccat
     2701 taccgagcgg aacgccccag aaacccccca gagcgcgagc aggatggaga taatcgcccc
     2761 gaaagtaaag gcgctctcgg cctggctctg cgtgatgctg aggagttgac cctcgacaaa
     2821 cttcgtggcc gattgcggcg ctataccaga gagcttgtcg accatgatgt tgacgaggtc
     2881 ggtcgcgttg aagagcccca aaagcgaaag caagaaggtg aaaaacggga agatcgcgaa
     2941 aaggcctttg taggtgaggt tgccagcaaa tgccatgaca tggtcttcgt caatctcctt
     3001 gtaggtcagc ttcaaaaagt cgacgagacc caggttctcc gcgacgggga cctcgacctc
     3061 gttgcctttg ctctcggcct tcttg
//
LOCUS       DATMJA010000005         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_414947, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000005 DATMJA010000000
VERSION     DATMJA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414947"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..571
                     /locus_tag="VNT12_00110"
     CDS             185..571
                     /locus_tag="VNT12_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWI44537.1"
                     /translation="MQNDEIDQQFVESQRERLLGLREELVRIKEGMADDERDLGEAEG
                     DTQLDSGDLSQDMFTREMDASIGEQVRRRLGEVDRALQKIEEGTYGLSDDSGESIPKG
                     RLEAVPEAIRTVEEQQRFEQERRPPI"
     gene            597..1466
                     /locus_tag="VNT12_00115"
     CDS             597..1466
                     /locus_tag="VNT12_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWI44538.1"
                     /translation="MDHRSSGDETREPIFRSDLEGPRPEPRYEPLRPEGGLWSVIKRW
                     VAPLAAVGLLLAKFKGLALLLLKVKFLGTALTMLVSIGAYALLFPVWFAVGIVVLIWV
                     HEMGHVVQLRREGIPASAPMFIPFLGAFVAMKQMPKDALAEARVGLAGPVLGTLGGLA
                     TLGLYAATREPLFLGLAYFNFIINLFNLAPLLPLDGGRAVGAMSPVLQLLGLLVMLAL
                     FFVAPIMAFIALLGLPELWHRWKTRNTPEGRAYYDLPLRQRAAVGVVYLGLIAVLGLL
                     TGATFVPDPAALQ"
     gene            1468..>2051
                     /locus_tag="VNT12_00120"
     CDS             1468..>2051
                     /locus_tag="VNT12_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HWI44539.1"
                     /translation="MRTILYTGKGGVGKTSVAAATALKASLAGKKVLVMSTDPAHSLA
                     DVFDTEIGQDPKEMAPGLWAQEMDHTSMIEDSWAEIQGYMTTLFEWQGADTLAAEELA
                     MLPGMDELFGLLMVRRHNQEERYDALILDAAPTGETLKLLSLPDHMNWYVEKIFPIQR
                     RAAKLVRPFASRARSLPPLPEDSVFAAGQRFYEA"
BASE COUNT          388 a          621 c          673 g          369 t
ORIGIN      
        1 cgcatcgagc gcctcgtctc gatcgggcgc cggccgccgc agccgcccgc aggggccaaa
       61 cctcgtctcg tggcctcagc tacggtgtag ttgtggccgt gcagcgtgtc tcggacagag
      121 tattagggta aacataggca acgtcgcttt ctagtagatt ggaagagatc cggaggaaaa
      181 acctgtgcag aacgatgaga tagaccagca gttcgtcgag agccagcgag agaggctgct
      241 tggtttgcgc gaagaactgg tgcgcataaa ggaaggcatg gccgatgacg aaagggacct
      301 cggcgaggct gagggggaca cgcagctcga ctctggcgac ctgagccagg atatgttcac
      361 ccgcgagatg gacgcctcca taggggaaca ggtccggcgc cgcctcggag aagtcgaccg
      421 ggcgctgcag aagatagaag agggcaccta cgggctctcc gacgacagcg gggagtccat
      481 acccaagggc aggctcgagg ccgtgcccga agcgataagg accgtcgagg agcagcagcg
      541 cttcgagcaa gagagacggc ccccgattta gggttacctg acggtataat ctgtctatgg
      601 atcatcgttc gtcaggcgac gagacaagag agcccatctt ccgcagcgac ctcgaagggc
      661 cgaggccaga acccaggtat gagcccctga ggccagaagg cggcctgtgg agcgttatca
      721 agcgctgggt tgcgccgctc gccgcggtag ggctcttgct cgccaagttc aagggcctgg
      781 cgctgctcct tctgaaggtc aagtttctgg gcactgcgct gaccatgctc gtcagcatag
      841 gggcttatgc cctgctgttt cccgtctggt tcgcggtcgg aatcgtcgtc ctgatctggg
      901 tgcacgagat ggggcacgtc gtgcagctaa ggcgcgaagg catcccggcc tcagcaccga
      961 tgttcattcc ctttctcggg gcattcgtcg cgatgaagca gatgcccaaa gatgccctgg
     1021 ccgaggcacg cgtcgggctc gccgggcctg tcctcggtac gctagggggg ctcgcgaccc
     1081 tcgggctcta cgccgccacg cgggaaccgc tgttcctggg gctcgcatac ttcaacttca
     1141 tcatcaacct gttcaacctc gcgccgctac tccctcttga cggcggcagg gccgtcgggg
     1201 cgatgtcccc ggtcctccag ttgctcgggc tcctcgtgat gctcgcgctg ttcttcgtgg
     1261 ccccgatcat ggcttttatc gcgctgctag gcctgcctga gctctggcac cgctggaaga
     1321 cgcgtaatac gccggagggc cgggcgtatt acgaccttcc gttgcgccag cgtgccgcgg
     1381 ttggcgtggt ctaccttggt ttgatcgcgg tactcggact gctgacaggg gctacgttcg
     1441 tgccagatcc ggcggcgctc cagtagcttg cgcacgattc tctacacagg gaagggcggc
     1501 gtcggcaaga cgagcgtggc tgcggcaacg gccctgaagg cttcgcttgc aggaaagaaa
     1561 gtgctcgtga tgagcaccga ccctgcgcac tcgctagccg acgtcttcga cacggagata
     1621 gggcaagacc cgaaggagat ggcccctggc ttgtgggcgc aagagatgga ccacacctcc
     1681 atgatcgaag acagctgggc cgagatacag ggctacatga ccacgttgtt cgagtggcag
     1741 ggcgccgaca cgctcgccgc cgaagagctg gcgatgctgc cggggatgga cgagctcttc
     1801 gggctactta tggtcaggcg ccacaaccag gaggaacgct acgacgccct gatcctcgac
     1861 gctgcgccta cgggcgagac gctaaagctc ctcagccttc ccgaccacat gaactggtac
     1921 gtcgagaaga tcttccccat acaacgccgc gccgcgaagc tggtcaggcc ttttgcgagc
     1981 agggcgagat ccctgccgcc gctgcccgaa gacagcgtct tcgccgcggg gcagaggttc
     2041 tacgaggcca t
//
LOCUS       DATMJA010000006         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_533500, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000006 DATMJA010000000
VERSION     DATMJA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533500"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VNT12_00125"
     CDS             complement(<1..140)
                     /locus_tag="VNT12_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI44540.1"
                     /translation="MREEVAVVTGAARGIGRRVALVLAKEGFRIVANDLAAPEETLEE
                     LRS"
     gene            complement(150..452)
                     /locus_tag="VNT12_00130"
     CDS             complement(150..452)
                     /locus_tag="VNT12_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44541.1"
                     /translation="MTEPVRDQWAEWLLERRFGGDSGQRERMLEGLISGESLGLELLL
                     KSSGNPKIPTLEEAMNEALTPEEIEHFTTHLRPLVERNESKGMSAVAYLWAVKQEG"
     gene            complement(464..1432)
                     /locus_tag="VNT12_00135"
     CDS             complement(464..1432)
                     /locus_tag="VNT12_00135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWI44542.1"
                     /translation="MGESVDEKVLVTREIPPAGLRALEPFDVRVLHERPPDREELLDA
                     VRGVSGVLSTATENVDGELMDAAGEDLRVVANMAVGYDNVDVEAAAERGVVVTNTPGV
                     LDETTADVAFMLLLAAARRLGEGERLLRAGRWEWWGPKQLMGRDVWGKRLGIVGFGRI
                     GQAVARRSKGFGMEILYHNRSRKEEAEQELGARYLDLDELLESADFVSVHTPLSDETH
                     HLIGAAELGRMKPEVIFVNTSRGPVVDEAALAGALAEGRIFAAGLDVYEEEPEVHPKL
                     LELDNVVLAPHIGSASVETRDRMAALAAENIVAVLSGEEPKTPVGR"
     gene            complement(1442..>2827)
                     /gene="acnA"
                     /locus_tag="VNT12_00140"
     CDS             complement(1442..>2827)
                     /gene="acnA"
                     /locus_tag="VNT12_00140"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HWI44543.1"
                     /translation="ITSCTNTSNPSVMMGAGLLARKAVEKGLSVAPHVKTSLAPGSKV
                     VTEYLQTSGLLDPLEKLKFDVVGYGCTTCIGNSGPLAEEISAAVEENDLVVAAVLSGN
                     RNFEGRINPDVRANYLASPPLVVAYALAGTVDIDLSKDPIGEDEDGNPVYLQDIWPSQ
                     EEVAREIENALDPEIYKEQYADVYTGNEQWNDVEVPSGDLYEWDPDSTYIQEPPFFKD
                     MGPEAQDLEDIEGARVLVKVGDSVTTDHISPAGAIPSRMPAGQYLIQKGVDPRDFNSY
                     GSRRGNHEVMVRGTFGNIRLRNQLVNKEGGYTLHFPDGEETTIYEASLRYGEENVPLI
                     VLAGKEYGSGSSRDWAAKGSFLLGVRAVIAESFERIHRSNLIGMGVLPLQFSEGESAE
                     SLGLTGHESFDIAGLAGLEPGKVLTVKVTADEGEQKEFEVKARVDSPVEVEYLRNGGI
                     LQTVLRQMLEE"
BASE COUNT          450 a          952 c          869 g          556 t
ORIGIN      
        1 gagcgcagtt cctcgagcgt ctcctcgggc gcggcgaggt cgttcgcgac tatccggaac
       61 ccctccttgg ccagcaccag ggctacccgt cgcccgatac cgcgggcggc cccggtgacg
      121 acggcgacct cttctctcac cttcaatgtt catccctcct gtttgacggc ccataggtag
      181 gcgacagcgg acatcccttt gctctcgttg cgctccacca gcgggcggag gtgggtggtg
      241 aagtgctcga tctcctcggg ggtaagcgcc tcgttcatcg cctcctctag cgtggggatt
      301 ttggggttcc cggaactctt gagcaggagc tccaagccga gactttcgcc ggagatcaga
      361 ccctccagca ttctttcccg ctgcccggag tcgccgccga agcgccgctc cagcagccac
      421 tccgcccatt ggtcgcgtac cggctccgtc atggccggac tctttatctg ccgaccggtg
      481 tcttcggctc ctcgccggag aggacagcga cgatgttctc agccgcgagc gccgccatcc
      541 tgtcgcgggt ctcgacggaa gcgctgccga tgtgcggggc gaggacgacg ttgtcgagct
      601 ccaggagctt cgggtggacc tcgggctcct cctcgtacac gtcgaggcct gcggcgaaga
      661 tacggccctc ggcgagggca ccggccagcg ccgcttcatc caccacgggg ccacgcgagg
      721 tgttgacgaa gatgacctcg ggtttcatcc ttcccaactc cgccgccccg atgagatggt
      781 gggtctcgtc ggagagcggg gtgtgcactg agacgaagtc cgcggactcc agcagttcgt
      841 cgaggtccag gtagcgagct ccgagctctt gctcagcttc ctctttccgg gagcggttgt
      901 ggtacaggat ctccattccg aaacctttcg agcggcgggc gacggcctgc ccgattcgac
      961 caaacccgac gatgccgagc cgcttacccc acacgtctcg ccccattagc tgcttcggac
     1021 cccaccactc ccaccgtccg gcgcgcagca ggcgctctcc ctcccccaga cggcgcgcgg
     1081 cagcgagcag caacatgaac gccacgtcgg cggtggtctc gtcgagcacg cctggcgtgt
     1141 tggtcacgac gacgcccctc tcagctgccg cctccacgtc cacgttgtcg tagcctacgg
     1201 ccatgttggc gaccaccctc aagtcctcgc cagcggcgtc cattagctcc ccgtccacgt
     1261 tctccgtagc ggtcgagagg acgccggata cgccgcgtac ggcatcaagc agctcctcgc
     1321 ggtccggcgg acgctcgtgc aggaccctca catcgaaagg ttcgagggcg cggagcccgg
     1381 cgggcgggat ctcacgggta acgaggacct tctcgtccac agactccccc atatccgatc
     1441 tctactcttc gagcatctgt cgcaatacgg tctgcaggat gccgccgttg cggaggtact
     1501 cgacctcgac cggtgagtcc acgcgcgctt tcacctcgaa ctccttctgt tcgccttcgt
     1561 cggccgtgac cttgacggtc aacactttgc caggctcgag gccggcaagg ccagcgatgt
     1621 cgaaggattc gtgacccgtg agaccgagag attctgcgct ctcgccctcg gagaactgca
     1681 agggcagcac gcccatgccg atcaggttag agcggtggat gcgctcgaag ctctccgcga
     1741 tgacggcccg caccccgagc aggaacgaac ccttcgccgc ccagtcgcgc gacgaaccgg
     1801 acccgtattc cttgccggcc aggacgataa gcggcacgtt ttcctcgccg tatctcagac
     1861 tcgcctcgta gatcgtggtc tcttccccat cggggaagtg gagggtgtag ccgccctctt
     1921 tgttcacgag ctggttgcgc agcctgatgt tgccgaacgt gccgcgtacc ataacctcgt
     1981 ggttgccgcg cctggagccg taggagttga agtcgcgcgg gtctacgccc ttctgtatca
     2041 ggtactgccc cgccggcatc ctcgaaggga tggcgcctgc tggcgagatg tgatcggtgg
     2101 tcacagagtc accaaccttc acgaggaccc tcgcgccctc tatgtcttcc aggtcttggg
     2161 cttcgggtcc catgtccttg aagaaagggg gttcctggat gtaggtcgag tcggggtccc
     2221 actcgtagag gtcgccagaa ggcacctcca cgtcgttcca ctgctcgttg cccgtgtaca
     2281 catcggcgta ctgctctttg tagatctcag ggtcgagggc gttctctatc tccctggcca
     2341 cttcctcctg tgaaggccag atgtcctgaa gatacacggg gttgccgtcc tcgtcctcgc
     2401 ctatagggtc tttagaaagg tctatgtcca ccgtgcctgc cagggcgtag gcgacgacga
     2461 gcggcgggga ggccaggtag tttgcgcgga cgtcggggtt gatgcggccc tcgaagtttc
     2521 ggttgcccga gagaacggcc gcgacgacga ggtcgttctc ctctacggcg gcggagatct
     2581 cctcggccag cggacccgag ttcccgatgc acgtcgtaca cccgtagccg accacgtcga
     2641 actttagctt ttcgagcggg tcgaggagcc ccgaggtctg cagatactcc gtgacgacct
     2701 tggaccccgg cgccaggctg gtcttgacgt gcggcgcgac ggagagcccc ttctcgacgg
     2761 ccttcctggc caggaggccg gcacccatca tcacggaagg attagaggtg ttggtgcagc
     2821 tcgttat
//
LOCUS       DATMJA010000007         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_829992, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000007 DATMJA010000000
VERSION     DATMJA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829992"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..1261)
                     /locus_tag="VNT12_00145"
     CDS             complement(263..1261)
                     /locus_tag="VNT12_00145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWI44544.1"
                     /translation="MLYIYVLVGVAILFVVGCDPAEPVANVDSQARKLVTFEGGDVTQ
                     GELDEFAEQSGLDTSMPKDDPQYKAAVQQIMPQLVGIEIAKAYAQEHNITVSDEEVDQ
                     EIAKIKEQVGEQARSSGQDVSNQEAYELALKQNNITEDQLREDIRENLLPIQKVQERV
                     TGDAEPSDKEIQNYYEKNKEAQFTTPEQRCVRHILFNKDQKEKAEDVKKQLENGGDFA
                     KLAKENSQDPGSAKNGGDLGCLGKGETVPEFEQAAFGAKQGEIVGPVKTEFGYHILKV
                     TDVKPEQTRSLQEVESQIRSQLATEKQSEEFSKWIEEQKKERDVKYLKGYEPPDQG"
     gene            complement(1354..>2599)
                     /locus_tag="VNT12_00150"
     CDS             complement(1354..>2599)
                     /locus_tag="VNT12_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HWI44545.1"
                     /translation="KDLGLVVVDEEQRFGVRHKERIKEFKSSVDVLTLTATPIPRTMQ
                     MGLASLRDISVIETPPAGRRSILTHVGPYDEELVRHAIEREVARGGQTFFVHNRVESI
                     DEVAERLRDLLPGVRFLTAHGQMPERALEGVMKHFLDGQADVLVTTTIVESGLDVATA
                     NTLVVDRSDAMGLAQLYQLRGRIGRSTEQAYAYLFAPLGATVESQKRLEALMDFTELG
                     SGFAIAMRDLEIRGAGNLLGAEQSGHIAAVGFEMYLTLLEEAVAAARGEPEERREERP
                     VIVELTLDAYLPPGYVMDEIERVDLYRRASGLGSLAEVEDLAEELVDRFGALPEPARN
                     LLGLSRLKLLAREVGATSVTYRSDNLTITGATAGSAAPALIRKSTGGTVTTRTGRTSV
                     RAPGSNPLFLAEESLRALARAG"
BASE COUNT          426 a          840 c          760 g          573 t
ORIGIN      
        1 tctgcgatga tctccgtcat tatcctcccc taattttagg tttcaggctt cagccgtcat
       61 gtcttgtctt cgggcaccgt gccgacgctc gcagatcttt acccgtcctc accgattctt
      121 accaacgaca cgcctcttgc caaccattct gagcgtaact ctacgcgcta atgtcgcgtg
      181 gcgccaccaa gagagcgggt ttacaggccc ttgctcctcc cacgggcagt ttacctgagg
      241 tctgaaacct gtagcctgat gcctacccct gatccggcgg ctcgtagccc ttcaggtatt
      301 tgacgtcgcg ctcttttttc tgctcctcga tccacttgct gaactcctcg gactgcttct
      361 cggtagcgag ttgggaccta atctgcgact cgacctcctg aagtgagcgg gtctgctctg
      421 gctttacgtc cgtaaccttg aggatgtggt agccgaactc ggtcttgacg ggaccgacga
      481 tctcaccctg cttggccccg aaggcagcct gctcgaactc gggtacggtc tcgcccttac
      541 cgaggcaacc gaggtccccg ccattcttgg cgctgcccgg gtcctgggaa ttctcctttg
      601 cgagcttcgc gaaatccccg ccgttctcca actgcttctt tacgtcctcg gccttctctt
      661 tctggtcttt gttgaacagg atgtgcctga cgcaacgttg ctcgggggtc gtgaactggg
      721 cctctttgtt cttctcgtag taattctgga tctctttgtc ggagggctct gcgtcgccgg
      781 tcaccctttc ctgtaccttc tggatcggga ggaggttctc gcggatatcc tcgcggagct
      841 ggtcctccgt gatgttgttc tgcttcaagg ccagctcgta ggcctcctgg ttgctcacgt
      901 cctgccctga ggagcgggcc tgctcgccga cctgctcttt gatcttcgcg atttcctggt
      961 cgacctcttc gtccgagacc gtaatgttgt gctcctgggc gtaggccttg gctatctcga
     1021 tgccgacgag ctgaggcatg atctgctgta cggcggcctt gtactgcgga tcgtctttgg
     1081 gcatgctggt gtccaaaccg ctctgctcgg cgaactcatc caactccccc tgcgtgacgt
     1141 cgccgccctc gaaggtcacc aatttcctgg cctgggagtc gacattggcg accggctcgg
     1201 caggatcgca gcccaccacg aagagtatgg cgacccccac taacacgtag atgtacaaca
     1261 tcctattctc aaaaaacgtg ctcaagacga cgaagaacct cgctgtatag atgccagaga
     1321 ccgcccggga ttatagcacg caggatgaca ggatcagcct gcccgcgcga gcgcccgcag
     1381 tgactcttcc gccaggaaga ggggattcga cccaggggcg cgtaccgatg tcctgccggt
     1441 tctcgtcgtt acggttcccc ccgtggactt ccttatgagg gcgggggcgg cggagccggc
     1501 cgtggctccg gtgatggtga gattatctga acggtatgtg acagaagtag cgccaacctc
     1561 ccgcgcgagg agcttgagcc gtgagaggcc gagaaggttt ctggccggct cgggaagcgc
     1621 accgaaacgg tcgacgagct cctcggccag gtcttccacc tcggcgaggg acccgaggcc
     1681 tgaagccctg cggtagaggt ctacgcgttc gatctcatcc atgacgtacc ccggcggcag
     1741 atacgcgtcg agcgtgagct ccacgataac aggccgctcc tcacgccgct cctcgggctc
     1801 gccccttgcg gccgcgacgg cttcttccaa aagggtcagg tacatctcga agccgacggc
     1861 cgctatgtgc cccgactgct cggcgccgag caggtttccc gcgcccctga tctccagatc
     1921 ccgcatggcg atggcgaacc cgctccccag ctccgtaaag tccatcaacg cttcgagccg
     1981 cttctgcgac tcgaccgtcg ctcccagagg ggcaaacaag taagcgtagg cctgctcggt
     2041 ggagcgtccg atcctgcccc gaagctggta cagctgggcg agccccatag cgtccgagcg
     2101 gtcgacgacc agggtgttcg ccgtggcgac gtcgagtccg ctctcgacga tggtggtcgt
     2161 gacaaggaca tcggcctgac cgtcgaggaa gtgcttcatc actccctcga gggcccgctc
     2221 aggcatctgt ccgtgcgcgg ttaagaaacg aacgccgggc agaagatcgc gcagcctctc
     2281 tgcgacctcg tctatgctct ccacgcggtt gtggacgaag aaggtctgtc cgccacgggc
     2341 gacctcccgt tcgatggcat gccttacgag ttcctcgtcg tagggaccaa cgtgcgtgag
     2401 gatgctgcgg cgtcccgccg ggggagtctc gatcacgctt atgtcgcgca gggaggcgag
     2461 gcccatctgc atggtgcgcg ggatcggggt cgccgtcagc gtgagcacgt ccacggaact
     2521 cttgaactcc ttgatgcgct ccttgtgccg gacgccgaag cgctgctcct cgtcgaccac
     2581 gacgagcccg aggtctttg
//
LOCUS       DATMJA010000008         5026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_415289, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000008 DATMJA010000000
VERSION     DATMJA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415289"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VNT12_00155"
     CDS             complement(<1..568)
                     /locus_tag="VNT12_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44546.1"
                     /translation="MSFLLKWSGTLCVPGGLLWALSPTGVSISTLAFHTPNVFWKMFW
                     PAPLLILLGLVGLQLRQSERSGLLEKLGFFVAIVGLVLIIAGDVGLYWLGIDDIFIVT
                     APAYNAFRIGLLVLAAGSILFAVTSPRDGALPTWGLLPFVLGSMCGLISFSRDFGSFG
                     AVLWALFGVGWAWLGLSLVVGGFSSFLRT"
     gene            complement(626..2116)
                     /locus_tag="VNT12_00160"
     CDS             complement(626..2116)
                     /locus_tag="VNT12_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWI44547.1"
                     /translation="MKGRTLTRRDFLKVAGAGAAGATMLGAAGCGSLANSFTQVPDEY
                     LPSGGSRMNVILVIIDSLRRDHLGVYGNDWIKTPNLDALAEVSLRFDRSHPESLPTIL
                     ARRAIHTGIRTFPFRDWQKWYDEDVNLWGWQPIPRGQVTLAEILLGEGFYNLIVTDTL
                     HQFRPFYDMHRGFHAFDFIRGQERDYFRPPSPASEKKMENTLIGGPSAAHSEEIMHQY
                     YANTVGRQREEDWFSPQVFTKAAEYLEMAKESQPFFMLVDNYDPHEPWDPPQKYIDLY
                     SDGFHGPEPVTSSSGPSDWLTESQLKRMHARYSAEVTMADYWLGHFLDKAEGLGILDN
                     TMLILLSDHGHAFGEHGYAGKVPGALYPELIDTIFFIRHPGGKGAGQTNDHYASTHDV
                     APTILGAMGLEPHPQMGGQDLSIVLDGKEPDPRPHFTAGYHDHVWTRDEKYAMFCLYN
                     GSDPHLFDLENDPKMDKNIASSNPDVVKKMFDEYIIKDAGGPLPHY"
     gene            2380..3363
                     /locus_tag="VNT12_00165"
     CDS             2380..3363
                     /locus_tag="VNT12_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI44548.1"
                     /translation="MNWLITGGCGFIGTALIRSLTEEGGHTMRVVDNLSVGTREDLAA
                     AGKFVEVSSDGLGPMAPGEPVELVAGDITDEDLALRAARGADVIVHFAANTGVMPSVE
                     DPRGDCMSNVVGTLNYLEAARHAGVGRFIFASSGGTIVGEAEPPIHEKMPPHPVAPYG
                     ASKLAGEGYCSAYFQTFGVETVALRFGNVYGPLSGHKNSVVARFIKRAVEGEVLEIYG
                     DGTQTRDFIFVDDLIGAVRLSATVEDVGGEVFQIATSSETTVQELTDRLLPALAAEGI
                     ENIEVRKTPARRGEVRRNYADTSKAQRMLGWKAEVGLDEGLRRTVEWFLGR"
     gene            complement(3375..4637)
                     /locus_tag="VNT12_00170"
     CDS             complement(3375..4637)
                     /locus_tag="VNT12_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWI44549.1"
                     /translation="MLNSLKTRQGAGAGSVHIAASFAFAGALTFAFQSLCTHVLGKQG
                     FAPLALLWSATFLTVQVLWIGATQTLGRYVAEREAQGKDWGQVVSSVRHWQIGLVVAF
                     VLCAVLAGPLLTDELFEDPWLTFAFIVAVVLYAPEYFRRGIFNGHRQSYRLGAQIVVE
                     SSARVLIAAVLLVLGWGVFGPVIAIVLAPLIGVLAIRPAPVAPPKREGEPFSAWHAFR
                     FAAPVLACMAFAQVLMNGGPILARVLDGSDAQVSVFSAALIFTRIPQYVLSPAIGALL
                     PRASRVLSTEGRRAFDRFVAKALGAVGLVGMLMVAGTWMFGEWGLRLFAGAEFDTSLA
                     VLVSLATMAAFYLLSDALNQGLFALGQGRLAALGWFVGLLACGACLAALGVGVVERVA
                     YSLALGTFVAAVAQGMFYVGMRPGAVGR"
     gene            complement(4798..>5026)
                     /locus_tag="VNT12_00175"
     CDS             complement(4798..>5026)
                     /locus_tag="VNT12_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HWI44550.1"
                     /translation="TNGYRAIRVSELHKLNLREDRFNANEIILEALKHKLRFEQVPVS
                     MMSRAAGETKKPTKLAYPLGVFRVIISTWLR"
BASE COUNT          995 a         1566 c         1610 g          855 t
ORIGIN      
        1 tcgttcgcag gaaagaggag aagcccccga ccacgagcga gagacccaac cacgcccacc
       61 cgacaccgaa cagggcccac aacacggcac cgaaggagcc aaagtctcgc gaaaaggaga
      121 tcaggccgca catcgacccg aggacgaaag gcagcaggcc ccaggtaggc aaagcgccgt
      181 cgcggggtga tgtgacggcg aagaggatag atcccgcggc caagacgagc agcccgatcc
      241 gaaaggcgtt gtaagcgggc gccgttacga taaagatgtc gtcgatccct agccaataca
      301 gaccaacgtc gcccgcgatg atcaagacga gtcccacaat ggctacgaag aaacccagtt
      361 tctccaacag tccggaacgc tccgactgcc gcaactgcag cccaaccaga ccgaggagga
      421 tgagcagtgg agccggccag aacatcttcc agaagacgtt gggggtgtgg aaagcgagag
      481 tggagatgga gacgccggta ggcgagagcg cccacagtag gccaccgggc acgcaaagcg
      541 tgccgctcca cttgaggagg aatgacaaca gcctacgcaa tagttttgtt ccctctccgg
      601 tacggttgca tcgaggcgca cccgatcaat aatggggcaa ggggccgccg gcgtccttga
      661 ttatgtactc gtcgaacatt ttcttcacca cgtctgggtt cgacgaggcg atgttcttgt
      721 ccatcttcgg gtcgttctcc aggtcgaaga ggtgggggtc tgaaccgttg tagaggcaaa
      781 acatggcgta cttctcgtcc cgggtccaga cgtggtcgtg gtagccggcc gtaaagtggg
      841 gcctcgggtc tggttccttt ccgtcgagca cgatggagag atcctggccc cccatctgcg
      901 ggtggggctc gaggcccatc gcgcccagta tcgtgggcgc cacatcgtgg gtggaggcgt
      961 agtggtcgtt cgtctggccg gcccccttgc ctccgggatg cctgataaag aagatggtgt
     1021 cgatgagctc gggatagagc gcaccgggta ccttgccggc gtaaccgtgc tccccgaaag
     1081 cgtgcccgtg gtccgagagc agtatcagca tggtgttgtc cagaatcccg agcccttccg
     1141 ccttgtccag gaagtgaccc agccagtagt cggccatcgt cacctcggcg gagtagcgcg
     1201 cgtgcatccg tttgagctgg ctctcggtga gccagtccga gggaccgctg ctcgaagtga
     1261 cgggctcggg gccgtgaaag ccgtcgctgt agaggtcgat gtacttctgt ggcgggtccc
     1321 acggctcgtg cgggtcgtag ttgtcgacca acatgaagaa aggctggctc tccttcgcca
     1381 tctccaggta ctcggccgcc ttggtgaaca cctgcgggga gaaccagtcc tcctctctct
     1441 gacggcccac ggtgttcgcg tagtactggt gcatgatctc ctcggagtgc gcggcgctgg
     1501 ggcccccgat cagggtgttc tccatcttct tctcggaggc cggcgaagga ggcctgaagt
     1561 agtctctctc ctggcccctg atgaagtcga aggcgtggaa gccgcggtgc atgtcgtaga
     1621 agggcctgaa ctggtgcagg gtgtccgtga ctatcaggtt gtagaagcct tctccgagca
     1681 ggatctcggc gagggtaacc tgtcctctcg ggataggctg ccaaccccag aggttgacgt
     1741 cctcgtcgta ccacttctgc cagtccctga aggggaacgt ccttatcccc gtgtggatcg
     1801 cacgccgcgc caggatggtt ggcagggact cggggtgcga gcggtcgaag cgcaggctca
     1861 cctcggcgag ggcgtcgagg ttgggggtct ttatccagtc gttgccgtag acgccgaggt
     1921 ggtctcttct caggctgtct atgatcacca gtatcacgtt cattctcgat cccccgctcg
     1981 gaaggtactc gtcgggcact tgtgtaaagc tgttggccag gctgccgcac ccggccgcac
     2041 cgagcatggt agcccctgcg gcccctgcgc cggccacctt gagaaagtcc ctgcgcgtga
     2101 gggtacgccc cttcacccct cgcctccggt tacacgcggg caagcgagga ctccggacat
     2161 ctttggagca gcgagcgtct tcgcaaccat ctacaaggcc ttttctacta catcgaacca
     2221 gccgccgaag attgtagcga atagagtccc tttccgcagg agactcgcgc cggaggggag
     2281 gttggtctaa tgtatgcgcg gcaaacaaca ctcggcaagg aaggttatgc actcacgtac
     2341 aaagagctca gcaggtcctc tcttgagaga gcggtcttcg tgaactggct catcacgggc
     2401 ggctgcggtt ttatcgggac ggccctgata cggtctctta cggaggaagg cggccacacc
     2461 atgagggtcg tcgataacct ctctgtaggg acgcgagagg acctggctgc cgctgggaag
     2521 ttcgtcgaag tatcgtccga cgggctaggc cctatggccc ccggcgagcc ggtcgagctg
     2581 gtcgcgggcg acataacgga cgaggacctc gcgctacgcg ccgccagggg agccgacgtc
     2641 atcgttcact tcgcggcgaa cacgggggtg atgccctcgg tcgaagatcc tcggggcgac
     2701 tgcatgagca acgtcgtcgg cacgctcaac tacctagagg cggccaggca cgccggcgtc
     2761 ggacgcttta tcttcgcctc gagcggcggg accatcgtgg gcgaggccga gccgccaatc
     2821 cacgaaaaga tgcccccgca tcccgtcgcg ccctacgggg cgagcaagct cgctggcgag
     2881 ggctactgtt cggcgtactt ccagacgttc ggcgtcgaga ccgtggcgct tcgcttcggc
     2941 aacgtctacg gtcccctctc gggccacaag aacagcgtcg tcgcgcgctt tatcaagagg
     3001 gccgtggagg gagaagtgct ggagatatac ggcgacggca cccagacccg cgatttcatc
     3061 ttcgtggacg acctgatcgg ggccgtgcgg ctctcggcca ccgtcgagga cgtgggcggc
     3121 gaggtcttcc agatcgccac cagctccgag acgacggtgc aggagctgac ggacaggctc
     3181 cttcccgccc tcgccgccga aggcatagag aacatcgagg tccgcaaaac gcccgcccgc
     3241 cgcggggagg tgcgccgcaa ctacgccgac acctccaagg cacagcggat gctcggatgg
     3301 aaggccgaag tcggcctcga cgagggcctc aggcgcaccg tggaatggtt cttgggtagg
     3361 taaggtctct tcacctagcg accaacggct ccaggtcgca tccccacgta gaacataccc
     3421 tgcgccacgg cggcgacgaa ggtgccgagg gccagggagt atgcgaccct ctcgacaaca
     3481 ccaactccca gtgccgccag acaggcaccg caagcgagca ggccgacgaa ccacccgagc
     3541 gctgcgagcc ggccctgccc cagggcgaag agcccctggt tgagcgcgtc ggagagaagg
     3601 tagaaggcgg ccatggtcgc cagggagacg agcacggcca ggctcgtgtc gaactcagcg
     3661 ccggcgaaga gcctgagccc ccactcgccg aacatccagg tgccggcgac catcagcata
     3721 ccaacgaggc ccacagcccc gagcgccttc gccacaaagc gatcgaaggc ccttctgcct
     3781 tcggtggaaa gcacgcggct cgcacggggc agaagcgcgc ctatggcggg gctcaagacg
     3841 tactggggga tacgcgtgaa gatcagtgcc gcactgaaca cgctcacctg cgcgtcgctg
     3901 ccatccagga ccctcgcaag gatcggaccg ccgttcatca gtacctgcgc gaaggccata
     3961 caagccagca ccggcgcggc aaaccggaag gcatgccagg cggagaaggg ctctccttcc
     4021 cgtttcggcg gcgctaccgg agccggcctg atggcaagta cgccgatgag cggggctagc
     4081 acgatggcga tgaccggtcc gaagacaccc catcccaaga cgagcagcac ggcggcgatg
     4141 agcaccctgg cggacgactc gaccacgatc tgggcgccga gccgataaga ttgcctgtgc
     4201 ccgttgaaga tgccgcgccg gaagtattca ggcgcgtaga ggaccacggc gacgatgaat
     4261 gcgaaggtaa gccacgggtc ttcgaatagt tcatcggtga gcagcgggcc cgccagcaca
     4321 gcacagagca cgaacgccac caccagtccg atctgccaat gcctgaccga ggagacgacc
     4381 tgaccccaat cttttccctg cgcctccctc tcggcgacgt agcgccccag cgtctgggta
     4441 gcaccgatcc atagcacctg cacggtgagg aacgtggccg accacaagag ggcgagcggc
     4501 gcgaagccct gcttgccgag gacgtgagta cagaggctct ggaacgcgaa ggtcagcgca
     4561 cccgcgaagg cgaaggatgc cgcgatgtgg acgctcccgg cacccgctcc ctgccgtgtt
     4621 tttaggctgt tgagcacttc agtacgctcc ggctgacgcc tccgcttgtc agcacttcag
     4681 catttcaact tctcaggtct cgtggtcgga gacgcctctc aatgagtagg ggctcccctg
     4741 ccattctctc aagcacacga gtgtctcacc acacaaagct gaaatgctga agtgctctca
     4801 acgcagccag gtcgagatta tgaccctgaa gaccccgaga gggtaggcca gcttcgttgg
     4861 cttcttggtc tcgccggcgg cgcgactcat catcgagacg gggacttgct cgaagcgcag
     4921 cttgtgcttg agggcttcga ggatgatctc gttggcgttg aaccggtcct cgcgcaagtt
     4981 cagcttgtgg agctccgaga cccttatggc gcggtaaccg ttcgta
//
LOCUS       DATMJA010000009         4303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_652482, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000009 DATMJA010000000
VERSION     DATMJA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652482"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1332)
                     /locus_tag="VNT12_00180"
     CDS             complement(109..1332)
                     /locus_tag="VNT12_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI44551.1"
                     /translation="MAVTSERTRREKRPVDLIGAGSLLSDEEKQVRDRVREFVDREVI
                     PVAADYWDRAEFPFGLLPGLGELGLMGGVLPGEYGCAGWNNVAYGLAIAELARGSGSL
                     ATFLHVQSGLAMTAIHELGSEEQKRRWLAPMAKCEKIGCFGLTEPEAGSDPGSLSTTA
                     VEKDGGYALSGEKKWIGNASFSDVAVIWARTEDNKISGFLVETGNPGFQADVLPRKAS
                     QRSAWQTHITLEDCHVPEAARLPKAEGLGSTLSILTHSRYGVGWDGLGQATDCYEVAL
                     AYAKEREQFGRPIASFQLIQQKLVEMVNEISLSQLLSIHVGRLKDAGGLDPATVSMFK
                     MNNVAKARQIAALARDVLGGNGILLDYRVMEHMADIEGVYTYEGTNDVNTLIVGQAIT
                     GHRAFSPRPPQRAEE"
     gene            1608..2507
                     /locus_tag="VNT12_00185"
     CDS             1608..2507
                     /locus_tag="VNT12_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI44552.1"
                     /translation="MTITERTSIDYDISGEGPPLLLISGLGFGRWGWFKQVPAFSRRF
                     QTITFDVRGERRLENGVDDLVRDVVSLLDHLGVKKAHVVGASLGGFVGQELALLRPDL
                     VDRLVLVCTSYGGIGPESMSVQALSDMLGWGSLSPEGVVRRGLETATSDTYRAEHGDE
                     FEQIVSWRLADSPSGAAYYEQARAGAGFDLSRDVGHITSPTLVIHGAEDRYVPVANAA
                     ALAGAIPGARLRVLDHAGHLVFIERFADVNREVVSFLKPRGDSGRGARVRRVGWKDRL
                     LGRIGEVPRSLGRWWRRKWNSVF"
     gene            2619..>4303
                     /locus_tag="VNT12_00190"
     CDS             2619..>4303
                     /locus_tag="VNT12_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI44553.1"
                     /translation="MSEGKTGNRGSDVPPEMAPFDVWAEWMRANMGPMMATPGASVPW
                     LVMPGIRTGEEAAELPEGAVRNDPLLSTVEKLWDANPMQNVLPIDWVEITRALQTLWA
                     REMSDPARAMERATDFNARLFGATMRSWQEATDRLWGLGASREEDEGRPDKRFSAPDW
                     EQNPFYETLKESYLLASEYLLDEAEKTNGEDTPEQRRLKFHLKQFVDAMAPVNYLLTN
                     PAALKRAFETGGASLAEGARNLLSDLQEGRMSMTDANAFQPGENLAITPGKVVYRNEL
                     IELVQYEPMTEQVHETPVMFIPPWINKYYILDLQPKNSLVKYLVDEGFTVFMISWKNP
                     DASMEDTKFDDYMTKGPLAAAEVVRDITGSEKVNPVGYCVGGTLLAIALSWLAAGDDE
                     NPFGPPTFMVALHDFTEVGDTGVFIDEPQIEFMEQQMLERGYLDNRKMANMFNLLRSN
                     DLIWSNVVNNYLLGQKPPAFDLLYWNADGTRMARDAHSFFLRNTYLENNLIKPSKVEI
                     KGRPIDLSRIEGDIYAVGAEKDHIVPWASAWKISRLANGTTRFTLANSGHIAG"
BASE COUNT          812 a         1323 c         1357 g          811 t
ORIGIN      
        1 gcaagggccg aggctcaaag cctcggccct ttcgcttgtc gatgcgactc gtgcgggaag
       61 aatgccagca ggtggatgca ctgggagcga gaacggcctg gttcgtcgtt actcttcggc
      121 gcgttgcggg ggtcgagggg agaaggcgcg gtggcctgtt atagcctggc ccactatgag
      181 ggtattgacg tcgttggtgc cctcgtaggt ataaacgccc tcgatatcgg ccatgtgctc
      241 catcacgcgg tagtcgagca ggatgccgtt gccccccagg acgtctcgcg ccagcgcggc
      301 gatttggcgc gccttggcga cgttgttcat cttgaacatc gagaccgtgg ccgggtcgag
      361 tcctcctgcg tccttgaggc gtccgacgtg gatggagagg agttggctga gtgagatctc
      421 attgaccatc tccacgagct tctgctggat gagctggaag gaggctatgg gtcgcccgaa
      481 ctgctcccgc tctttagcat aggccagcgc aacttcgtaa cagtccgtcg cctgcccgag
      541 tccgtcccac ccaacaccgt agcgtgaatg cgtcaggatc gaaagggtcg agccaagacc
      601 ttctgcttta ggcagcctgg cagcctcggg cacgtggcaa tcttctagcg taatgtgtgt
      661 ctgccaggcg gagcgctggg aggccttgcg ggggaggacg tcggcctgga atcctggatt
      721 cccggtctcg acgaggaaac cggagatctt attgtcctcg gtcctggccc agatgacagc
      781 cacgtccgag aaggaggcgt tgccgatcca cttcttctcg ccgctcagcg cgtagccgcc
      841 gtccttctct acagccgtcg tgctcaaaga accagggtcg cttcctgctt ctggttctgt
      901 caggccgaag cagccgatct tctcgcactt cgccatgggc gccagccagc gccgcttctg
      961 ctcctccgat cccagctcgt gtatggccgt catcgctagc ccgctctgga cgtgcaaaaa
     1021 ggtggccaga ctcccagacc cccgtgccag ctcggctatc gcaaggccgt aagctacatt
     1081 gttccagccg gcacaaccat actcccctgg gaggacgcca cccatgaggc cgagctcccc
     1141 aagtccgggt aggagcccga aagggaactc cgccctgtcc cagtaatcgg ccgcgaccgg
     1201 tatgacctca cggtccacga actcgcggac ccggtccctg acctgcttct cctcatccga
     1261 gagcagactg ccagcgccta tgaggtcaac aggccgcttc tcccggcgcg ttctctccga
     1321 tgtaaccgcc attcggtttc ctccatgctc cgattccggg caccacccag cacccttctc
     1381 gggaagagca taggttctgg cggcgatccg gtcaatccaa gagtgatggc tttcacgcta
     1441 ccaacttgcc cctcatgcca gaccctctag aatgttctcg tgcggcatct aagtgcagag
     1501 tgcatctatc gttataaagg gagatgctgg tgcgggatct ggggcaaccg gttccgtttc
     1561 aagcagcctc tctgtggagc gagaatcgag gtgagtagcg aatgccggtg acgattaccg
     1621 agcgcacgag catcgactac gatatcagcg gggaagggcc gcccctgctc ctcataagcg
     1681 gccttggttt cggtcgctgg ggttggttca agcaggtccc cgctttctcg cgccgcttcc
     1741 agaccatcac cttcgatgtc aggggtgagc ggaggctaga gaacggcgtc gatgacctgg
     1801 tccgtgacgt ggtgtcacta ctggaccacc tgggcgtaaa gaaggcccac gtcgtcggag
     1861 cttcgctcgg cggctttgtg gggcaggagc tggcgttgct caggcccgac ctggtcgacc
     1921 gtctggtcct ggtctgcacc agctacggtg gcatcgggcc cgagtcgatg tctgtacagg
     1981 ccctttcgga catgcttgga tggggttccc tttctccaga gggggtcgtc agacggggcc
     2041 tcgagacggc gacatcggat acgtatcgcg ccgagcacgg agatgagttc gagcagatcg
     2101 taagctggcg tctcgctgac tcgccttccg gcgccgccta ctacgagcag gccagggccg
     2161 gggcgggctt cgacctctcc cgggatgttg ggcacattac ttcacccacg ctcgtgatcc
     2221 acggcgccga ggaccgctac gtgcccgtcg ccaacgcggc ggccctcgcc ggggcgatcc
     2281 caggcgccag gttgcgggtt ctcgaccacg cgggccacct cgtctttatc gagcggttcg
     2341 ccgatgtgaa cagggaggtg gtctccttcc tcaagcctcg cggggacagt gggcgaggcg
     2401 cccgggtacg gcgggtgggg tggaaggaca ggctcctggg taggatcggg gaggtgccac
     2461 ggtctctcgg gcgttggtgg aggaggaagt ggaacagcgt tttctgagct cccttgccgg
     2521 ccgtttttgg gccacaggca tagtgggtag aatagtacgg ttgaatacga gctggcggcg
     2581 ctggccgccg ggtaaatctt tctgaggagg aagactagat gagcgaaggc aagactggca
     2641 accgcggttc cgatgttccg cccgagatgg cgccgttcga cgtgtgggcg gagtggatgc
     2701 gggcgaacat gggccccatg atggcgacgc cgggcgcgag cgtaccgtgg ctcgtgatgc
     2761 cgggcatcag gacaggagag gaggccgcgg agttgcccga gggggctgtg cggaacgatc
     2821 ctttgctctc caccgtcgag aagttgtggg acgccaaccc catgcagaac gttttgccca
     2881 ttgactgggt cgagatcacc cgcgcgctgc agaccctctg ggcgcgcgag atgtccgacc
     2941 ccgcgcgcgc catggagcgg gccacggact tcaacgccag gctgttcggc gccacgatga
     3001 ggagctggca ggaggccaca gaccgtctct ggggcctagg cgcttcgcgg gaggaagacg
     3061 agggccgtcc cgacaagcga ttctcggcgc ccgactggga gcagaacccg ttctacgaga
     3121 cgctcaagga gtcatacctg ctcgcctcgg aatatctgct cgatgaggcg gagaagacca
     3181 acggcgagga cacgcccgag cagcggcgtc tcaagttcca tctcaagcag ttcgtagatg
     3241 ccatggctcc cgtcaattat ctgctgacca atcctgcggc cctcaagcgg gccttcgaga
     3301 ccggtggagc cagcctcgcc gagggcgccc gcaacctgct ctcagacctg caagaaggcc
     3361 gcatgagcat gaccgacgcc aacgccttcc agccgggtga gaacctggcc atcacaccag
     3421 gcaaggtcgt ctaccgcaac gaactcatag agttggtcca gtacgagccg atgaccgaac
     3481 aggtacacga gacacctgtg atgttcatac cgccctggat caacaagtac tacatcctcg
     3541 acctgcagcc gaagaacagc ttggtcaagt accttgtgga cgagggcttc acggtgttca
     3601 tgatctcctg gaagaatccg gacgcctcga tggaggacac caaattcgat gattacatga
     3661 cgaagggacc gctcgccgcg gccgaggtcg tgagggacat cacgggttca gagaaggtca
     3721 accccgtcgg ctactgcgtg ggagggacgc tgctcgcgat tgcgctctcc tggctcgccg
     3781 caggcgacga cgagaacccc ttcggcccgc cgacctttat ggtcgcgttg cacgacttca
     3841 cggaggtggg cgacacgggg gtcttcatag acgagccgca gatagagttc atggaacagc
     3901 agatgctcga gcgcggctat ctcgacaacc gcaagatggc caacatgttc aacctgctac
     3961 gctcgaatga cctgatctgg tccaacgtag tcaacaatta cctactcggc cagaagcctc
     4021 ctgccttcga tctcctttac tggaacgcgg atggcacgcg catggcacgc gacgcccaca
     4081 gcttcttcct gcgcaacacc tacctggaga acaacctcat aaagccaagc aaggtagaga
     4141 taaagggccg tcccatcgat ctatccagga tcgagggcga catctatgcc gtcggggccg
     4201 agaaggacca catcgtcccc tgggcctcgg cgtggaagat ctccaggctc gcgaacggca
     4261 ccacccgttt caccctagcc aactcgggcc atatagcggg cat
//
LOCUS       DATMJA010000010         3344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_297293, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000010 DATMJA010000000
VERSION     DATMJA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297293"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VNT12_00195"
     CDS             complement(<1..806)
                     /locus_tag="VNT12_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44554.1"
                     /translation="MTRDEGIERVAPNSANQADNAQQSDKEGQGQQQAQGEQQDGQGG
                     ADAPPQQEQQGDEAGSQEIKDNVPVRKNDPRTSVQKSEEYRYASNIPGDRTVPEQAYA
                     DYTLEYDEEDAVMNGKIPDVLLDIPVVKVDEINFELNDLRAQFSVHAKVLDLVELSIG
                     ADVYLGRVKLVIKGIEAQALLKARLDNLTAILDRVLTTIDRNPQIVEKLAESVGSAVE
                     DVGEGAGSAVEDVGQGAGQLVGEGVNSAVEDIGEGAGQLVGEGANQAVAD"
     gene            complement(985..1818)
                     /locus_tag="VNT12_00200"
     CDS             complement(985..1818)
                     /locus_tag="VNT12_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWI44555.1"
                     /translation="MRIDDLIEAFAAHLADDPGTSPRTAEFYGADLHEFALFLQERDV
                     SRIEDVGVGAVMEFRNDLLESNRKRFTVYRKDAAVRRFLDWARTSTDADIDFDPIEPM
                     HQPLDQPIVFLEADEVDQLLSFPLNTRDAARDAAVIRLMLDTGVTVSEIRGLRKSDVD
                     LEAAQVHLGGPGSHLAPRVRRLSGETVEAVRRYLGTRNDETQELIVARAARPVTTSKS
                     LQNAIWKRCDQVGMWRVSPMNLRHTFAVRLLRRGATLGELKEALGVRDTSNIGVYKKF
                     V"
     gene            1895..2497
                     /locus_tag="VNT12_00205"
     CDS             1895..2497
                     /locus_tag="VNT12_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWI44556.1"
                     /translation="MLVSLAILLCLLTLPILPEHETVSAKLRTTQVENTGTLLGENSP
                     QLEVPEKAPPVTPGGGKISAAMKITVPRLELKDVAVPTGSRQAELDREGILHLRGSGL
                     PWIAGSNTFITGHALGFPRTRVPYAFYKLDKMKPGDEIFIEDKDGQRYTFRVYDLLTV
                     EPNDYWTTYPVEGKTVVSLQSCTPIPTFKHRLIVRGELVS"
     gene            complement(2623..3081)
                     /locus_tag="VNT12_00210"
     CDS             complement(2623..3081)
                     /locus_tag="VNT12_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWI44557.1"
                     /translation="MSIPRRLGRLAQGFVSSLQEDERFRERLRTSRERSEALRGAFEA
                     AWKGASEEWRKAEERRAAQEAAAEQTWGWRSATTTFTPRKYPPNVLAAYDRLGLVPSA
                     SMEEVDRKRRELVKRFHPDRFSDPEKRLRAERVTAEINAAHDAIERHVAG"
     gene            complement(3078..>3344)
                     /locus_tag="VNT12_00215"
     CDS             complement(3078..>3344)
                     /locus_tag="VNT12_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44558.1"
                     /translation="ARDFGVSEEERKISSAESGLDNIDMVRDFFGLPQEEADEPPADP
                     AAGEETHVMEGVEDEEEDDTQVLRRPGPLSDSEDYLEPGDKKEQ"
BASE COUNT          526 a         1046 c         1036 g          736 t
ORIGIN      
        1 atatctgcca cggcctgatt ggcaccttct cccacgagct gtcccgctcc ttctccgata
       61 tcctctacgg ccgagttcac accctcgccc acgagctgac ccgcgccctg gcccacgtct
      121 tcgacggcgg agccggctcc ttcgccaacg tcctcgaccg ccgagcccac gctttcggcc
      181 agcttctcga cgatctgggg gttgcggtct atggtcgtga gaacccggtc gaggatagcg
      241 gtcaggttgt ccagcctggc tttcaggagc gcctgcgctt cgatgccctt tatgaccagc
      301 ttgaccctgc ccaggtacac gtcggccccg atggagagct cgaccaggtc gagcaccttg
      361 gcgtgcactg agaactgcgc ccgcaagtcg ttgagttcga agttgatctc atcgaccttg
      421 accacgggta tgtcgagcag cacgtcgggg atcttgccgt tcatcacggc gtcttcttcg
      481 tcgtactcca gcgtatagtc cgcgtatgcc tgctccggca ccgtcctgtc gcccggtatg
      541 ttgctcgcgt accggtactc ttcgcttttc tgaaccgagg tgcgagggtc gttcttacgg
      601 accgggacgt tgtccttaat ctcctgcgag cccgcctcgt ccccctgctg ctcctgttgc
      661 ggcggggcgt ccgctcctcc ttgcccgtcc tgctgttcgc cctgagcctg ctgctgtcct
      721 tggccttctt tgtccgattg ctgggcgttg tccgcctggt tcgcgctgtt gggtgccact
      781 ctctcgatcc cttcgtcccg cgtcatctgt tactgatagc ctactagtag gctttatatc
      841 ccacgcctcg atcgagtcca aacttcggct atgatccgac cggcgatacg ctgtgtagca
      901 acacgttctt tgttatcttg gacgcgagag tatcgttgcc ggttacgccg tggtcgccgg
      961 gtttcgccgc gggtgactgg ggatctagac gaacttcttg tacacaccta tgttggaggt
     1021 gtcgcggacg cctaaggcct ctttgagctc acccagggtg gcgcctcggc ggagcagcct
     1081 taccgcgaag gtgtggcgga ggttcatagg ggagacgcgc cacattccga cttggtcgca
     1141 gcgcttccag atggcgtttt gcagcgactt cgaggtcgta acgggccggg cagcgcgggc
     1201 gacgatcaac tcctgcgtct cgtcattgcg cgtgccgagg tagcggcgga cggcctctac
     1261 cgtctctccc gagagacgcc gtacgcgagg ggcgaggtgg gagccgggac cgccgaggtg
     1321 tacctgggcg gcttcgaggt cgacgtcgct ttttcgcagg ccacggatct cgctcaccgt
     1381 aacccccgtg tcgagcatga gccttatgac cgcggcgtcg cgggcggcgt cgcgggtatt
     1441 cagggggaag gagaggagct ggtccacctc atctgcttcc aggaagacta tcggctggtc
     1501 gagaggctgg tgcatcggtt cgatggggtc gaagtctatg tcggcgtccg tagaggtgcg
     1561 ggcccaatcg aggaacctcc tcacggcggc gtccttgcgg tacacggtga accgcttgcg
     1621 gttgctttcg aggagatcgt tgcggaactc cataacggcg ccgacgccca cgtcctcgat
     1681 cctgctaaca tctctctcct gaaggaagag ggcgaactcg tgaaggtcgg caccgtagaa
     1741 ctcggccgtg cgagggctcg tgccagggtc gtccgcgaga tgggccgcga aggcttcgat
     1801 caggtcgtct atcctcacgc gtatcattct aacggcaaat cagtgctgct tggtatactg
     1861 cctgagggtg ataaatactc caaaagcttc ggtcttgctg gtctcgctgg caatcttgct
     1921 gtgcctcctg accttaccaa ttctgccgga gcatgagacg gtctcggcga agctccgcac
     1981 gacccaggtc gagaacacgg gtacactgct cggagagaac tccccccagc tcgaggttcc
     2041 agagaaagct cctccggtca ccccgggcgg ggggaagatt tcggcggcca tgaagatcac
     2101 ggtgcccagg ctcgaactaa aggatgtggc cgtgccgacg ggatccaggc aggccgagct
     2161 agacagggag ggcattctgc acctgcgcgg cagcggccta ccctggatag cgggctcgaa
     2221 cacgtttatc acggggcacg ctttagggtt cccgcggacg agggtgccct acgccttcta
     2281 caaactagat aagatgaaac ctggcgatga gatcttcatc gaagacaagg acggacaaag
     2341 gtacacgttc agggtctacg acctgctcac cgtcgagccc aacgactact ggacaacgta
     2401 ccccgtagag ggcaagaccg tcgtctcgct tcagagctgc acccccatac cgacgttcaa
     2461 gcatcgcctt atagtgcgcg gcgaactggt ctcctagcac ttcagcgcgg cggctccgcc
     2521 gccttgtcag cacgctccct tcgggagctt tcagcatttc agctgttggc gttcgcgagt
     2581 ccttgctgac ttgcgcgggt tgaggaaggc tcaaggcctt cctcaacccg ctacgtggcg
     2641 ttctatggcg tcgtgggcgg cgttgatctc tgcggttacg cgttcggctc ggagtcgctt
     2701 ctctgggtcg gagaaacgat ccgggtggaa gcgcttgacc agctctctgc gtttcctgtc
     2761 tacttcttcc atggaagcgc tggggacgag gccgagcctg tcgtaggcgg ccaggacgtt
     2821 cggtgggtat ttcctgggag tgaatgtggt ggtcgcggac cgccatcccc aggtctgttc
     2881 tgcggccgcc tcttgtgcgg cacggcgctc ctctgccttg cgccactcct cggaggcgcc
     2941 tttccaggcg gcttcgaagg ctcctctcag ggcctcgcta cgctcgcggc tggtacggag
     3001 cctttcgcgg aagcgctcat cttcctgcag gctggagacg aaaccctggg cgaggcggcc
     3061 caggcggcgg gggatgctca ctgttccttt ttgtccccgg gttccaagta gtcctcggag
     3121 tccgagagcg ggcctggacg gcggagaacc tgggtgtcgt cttcctcctc gtcttccacg
     3181 ccctccatga cgtgcgtctc ttcaccggct gcggggtcgg cgggtggttc gtccgcctct
     3241 tcctgtggca agccgaagaa gtcgcgcacc atatctatgt tatcgaggcc ggactcggcg
     3301 gacgagatct tcctctcctc ttcggagacg ccgaagtctc tggc
//
LOCUS       DATMJA010000011         5893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_593647, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000011 DATMJA010000000
VERSION     DATMJA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593647"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1492
                     /locus_tag="VNT12_00220"
     CDS             125..1492
                     /locus_tag="VNT12_00220"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI44559.1"
                     /translation="MRSKLAVGGLILGAAWGAYWRLRKPISLQGSYAHAPTKILIIGG
                     GFGGLSVARELARALFGTEDVGVALVDRLNYTTFWPMVPSIIPSNAEVRHVAHSLRRI
                     LKPLGVEFFQDEVAGVDFGARRVKTAERDYPYDYLVLAPGSRTTYFGTPGAVENAMDV
                     KGLRDALRVRNHVIDRFEEAEHLDDHATDDLLTFVVVGGGPTGVEAAADMHDLIFDVL
                     EDDYPNVDFDRVRVVLVNAGDQILKGLDSSLVNAATRRLASQRIEVINDAKVEEVRPD
                     AVVISDGRTIPTQTTVWAAGIEPPPLVGNLEVQKDHRGRILIDQYLRVKGRPGVYAVG
                     DCTNLQYDGAPVPPLAQAAEQEGKRAASNLVAEIKNQTPVAFRYRSVGQLVDLGEGSA
                     LVDILGVKLGGLIGAFIWKGVYLYELGYDLNRAHVLADWTIDLFGRPDTSKLIEDSKQ
                     PKPGN"
     gene            1802..2191
                     /locus_tag="VNT12_00225"
     CDS             1802..2191
                     /locus_tag="VNT12_00225"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWI44560.1"
                     /translation="MPTSSPARQRLGPDRFPRTGRHQITSAAMIMVSVFLAFVINGDP
                     TIKQFGLGMAIAVAVDATVVRCVLVPAIMSLLGTSGWWLPKWMERVTPKFSIEGNEWF
                     AEHETPAEQANALQQESEERHEPDRAE"
     gene            2324..3253
                     /gene="holA"
                     /locus_tag="VNT12_00230"
     CDS             2324..3253
                     /gene="holA"
                     /locus_tag="VNT12_00230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWI44561.1"
                     /translation="MTVYLLLGDDEERKSRGVEKLRAGRTVEAYDASETSPETLVSAC
                     NSFSLFGEGPFVVLKNLDAWNAAQKAVIVDYLKDPSPGSDLILIGKKLGARERLLSAV
                     KNSGEVHAFDQPTGKALIRWLVGHANEMDLDLPEDVAEDLTNRCSGDKMRLLKETEKL
                     ALYVGDRTTTHDDVAALCPPDVQSNIFAFVDSLAAGERDRALALLEGLISTGEPPLRL
                     TFMIRRQFQLVARARALLDRGTPRKELSSRLKVPPFVARKLEEQGRKLDEEDLERALV
                     LIQHLEGGLKGGSYLSDELQVEMTVLGLSENSA"
     gene            complement(3414..3680)
                     /locus_tag="VNT12_00235"
     CDS             complement(3414..3680)
                     /locus_tag="VNT12_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44562.1"
                     /translation="MGEGDHTAKEEALRRLSEPEGDHELHSVLERMKVHHPHLWEALH
                     RVHFSHEGGPSRLEDWRRAPKGSQEEVWAEHYDEAMGVLAMDLH"
     gene            complement(3750..4007)
                     /locus_tag="VNT12_00240"
     CDS             complement(3750..4007)
                     /locus_tag="VNT12_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44563.1"
                     /translation="MGRSRGTGSQRFRSFMWIATALWLVGAIAAVIDRDTLNAVAWFG
                     FAAFGALTASGSTERSRGLAYLAIALLVVAMAILIGVFLAD"
     gene            4120..4269
                     /locus_tag="VNT12_00245"
     CDS             4120..4269
                     /locus_tag="VNT12_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44564.1"
                     /translation="MSAENEALVRRYVEEVFDQCKLEVIDELFASDFTLHDPEVQESF
                     RGLLG"
     gene            complement(4284..4907)
                     /locus_tag="VNT12_00250"
     CDS             complement(4284..4907)
                     /locus_tag="VNT12_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HWI44565.1"
                     /translation="MSEEAYPTGSVVLVPFPFTDLSGRKRRPALVISPEGFDDEDVVL
                     CAITSRLPERFSEWEASLGAEDIVEERLPKRSVVKVGKLFTMHRSLIARRFGTVKEYK
                     LEEILSKLRTFFARPPWISEPDSEDREVGLKTKEIDEAVLGLLYLNAFKDHGQVRAWK
                     TFDWDAMDRLHDLGLIGDPKSKAKSVPLTAEGVTLAKESFRNRFALQ"
     gene            complement(4913..5203)
                     /locus_tag="VNT12_00255"
     CDS             complement(4913..5203)
                     /locus_tag="VNT12_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWI44566.1"
                     /translation="MYSVGTRELKQHTGEIVERVRNGERVILTLRGEPVAVISPLDRR
                     AIEEAIEIDARRAERESLGWLATSESSFSFWDNEEDEVWDEEAEEPVDRARG"
     gene            complement(5550..5810)
                     /gene="rpsT"
                     /locus_tag="VNT12_00260"
     CDS             complement(5550..5810)
                     /gene="rpsT"
                     /locus_tag="VNT12_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWI44567.1"
                     /translation="MPAPSKRDKQSVKRGAHNKGVRTRLRNLSKNFYRAVESGDEEEA
                     RRIRDESQREYNKAAGKSIIHPNRAARKVSRLDKALATDRSE"
BASE COUNT         1178 a         1839 c         1745 g         1131 t
ORIGIN      
        1 aatcggaagc accaatccca aacggccaaa gacgaagcgg agggcaaggc tgatgagcga
       61 aggcgtcagc ctgagcgtgc tgaaatgcta tcctgcaaga gccatccgaa aggtagaaga
      121 caagatgaga agcaaactgg cggtgggcgg cctgatcctg ggcgcagctt ggggagctta
      181 ctggcggcta cgcaaaccca tctcgctcca aggaagctac gcgcacgccc cgaccaaaat
      241 tctcatcatc ggcggcggat tcggaggcct ctcggtggcg agagagttgg cgcgagcgct
      301 ctttgggacc gaagatgtgg gggtcgccct ggtggatcgc ctgaattaca ccaccttctg
      361 gccgatggtg ccttcgatca tcccgagcaa cgcggaggtg cgccatgtgg cgcactccct
      421 caggcgcata ctcaaacctt tgggagtaga gttctttcag gatgaggtgg caggcgtgga
      481 cttcggggct cgccgggtga agacggctga gcgcgattac ccctacgatt accttgtcct
      541 ggccccgggc agccgcacga cctacttcgg caccccaggc gcagtagaga acgccatgga
      601 tgtcaagggt ctgagagacg cgctgcgggt acgcaaccac gtgatcgacc gcttcgagga
      661 agccgagcac ctcgacgacc acgctactga cgacctgctc accttcgtcg tcgtcggcgg
      721 aggtcccacc ggggtcgagg ccgcggccga catgcacgac ctgatcttcg acgtgctcga
      781 ggatgactat cccaacgtcg acttcgaccg tgtgcgtgtc gtgctggtga acgccggcga
      841 ccagatcctc aaaggccttg attcgtcgct ggtaaacgcg gccacgcgca ggctcgcctc
      901 tcagcgcata gaggtcatca acgacgccaa ggtcgaagag gtcaggcctg atgccgtggt
      961 gatctcagac ggacgcacca taccaaccca gacaaccgtc tgggcagcag ggatcgaacc
     1021 ccctcctcta gtcggaaacc tggaggtgca aaaagaccat cgcggaagga tcctcatcga
     1081 ccagtacctg cgggtgaaag gcagaccagg agtctatgct gttggcgatt gcaccaactt
     1141 acagtacgac ggagcaccgg tcccacccct cgcccaggcc gcagaacagg aaggcaagag
     1201 ggcagcgtcg aaccttgtag cagagatcaa gaaccagaca cccgtggcgt tccgttaccg
     1261 ttccgtaggc cagctggtgg atctcgggga gggtagcgcc ctcgtcgaca tcctcggagt
     1321 aaagctcggt ggtctgatcg gggcgttcat ctggaagggg gtttacctgt acgagctcgg
     1381 ctacgacctc aaccgggcgc acgtactcgc cgactggacc atcgatctct tcggccgccc
     1441 ggacacctcc aaactcatcg aagacagcaa gcagccgaaa cctggtaact gatgcctaat
     1501 acgccagact agcccatttg agggatgcgg cgccccatcg cgggcgtgta tgctgccatt
     1561 gggagacccg atcgagaaac agatgcgtgg caagacgtgt agaccacagc acataaggag
     1621 accgtgttga cgccactgct ctaccgtctg ggttactttt gcgcccgtca ccccgtgcgg
     1681 gtccttgtag tctggctggt ggtcgcactc gcgatcgtgg gtgtggccag gagcgtcggc
     1741 caggagacga acgacaacct ctcgctgccc ggtactgaca gccaggcggc cagcaacctg
     1801 cttgccgaca agttccccgg ccaggcaacg gctcggtccc gatcgctttc cgcgcaccgg
     1861 ccggcaccag atcacctcgg cggcgatgat catggtctcc gtcttcctcg ccttcgtgat
     1921 aaacggtgac cccaccatca aacagtttgg tctcggcatg gccatagcgg tcgcggtcga
     1981 tgccaccgtc gtccgctgtg tgctcgtacc cgcgatcatg tctctgctcg ggacctcggg
     2041 ctggtggctg ccgaagtgga tggaacgtgt gaccccgaag ttctcgatcg aaggcaacga
     2101 atggttcgcc gagcacgaaa ccccggcgga gcaggccaac gcgttacaac aagaatctga
     2161 agagcgccac gagccagacc gcgccgaatg acccacgccc acctcaaatc ggtcgaacca
     2221 tcgaggggaa cgtatcgcct gctcatcccc acgtccgttt gcagagaagg cctgaaacct
     2281 gattctggta ctcgcgggta cctctagcga ttagaatacg tttatgacag tctacctgtt
     2341 actcggagac gatgaggaac ggaagtcccg cggtgtcgag aagctgcgtg ccggaaggac
     2401 ggtcgaggca tatgacgcct cggagaccag tccggaaacg ctcgtctcgg cctgcaactc
     2461 cttctccctc ttcggagaag ggcccttcgt agtcttgaag aacctcgacg cctggaacgc
     2521 ggcccagaag gcggtgatcg tcgactacct caaggaccct tcgccgggat cagacctgat
     2581 cctgatcggc aagaagctcg gcgcccgtga gaggctgctc tcggcggtca agaactcggg
     2641 agaggttcat gccttcgatc agcctacagg caaggccctc atcagatggc tcgtcggaca
     2701 cgcgaacgag atggacctgg atctccccga ggatgtcgca gaagacctca ccaatcgctg
     2761 ctcgggcgac aagatgcgtc tgctgaagga gacggagaag ctggcgctct acgtcggtga
     2821 tcgcacaact acccacgacg acgtggccgc cctgtgtcca cccgacgtcc agtccaatat
     2881 cttcgccttc gttgattcct tggccgccgg ggaacgagat agagcactcg cgttgctcga
     2941 gggcctgatc agcacgggcg agcccccgct acggctcacg ttcatgatcc gtcgccagtt
     3001 ccagctcgtc gcccgcgccc gcgccctgct cgaccgcggc actccccgaa aggagctctc
     3061 cagcaggctc aaggtcccgc cgttcgtggc gcgcaagctc gaggaacagg gccgcaagct
     3121 agatgaggag gacctggaac gtgccctggt cctgatccag cacctcgaag gcggcctcaa
     3181 aggggggagt tatctctccg acgaactcca ggtggagatg accgtgctag gcctctccga
     3241 gaattccgca tgaccccgaa ggatacgtat aacgtcaggc tcgtcgaact ggacgacgcg
     3301 ccataccatg agcacagaaa ggatctggtg cgcgattacg cggcggatta agttcgcgcc
     3361 gacgcctggt cacaggccga gggccgtgcg gaatccactg cgccaggatt gggctaatgc
     3421 agatccatcg ccaggacgcc catggcctcg tcataatgct cagcccagac ctcctcctgg
     3481 ctacctttag gagctcttcg ccaatcctcg agcctcgagg ggccgccctc gtgtgagaag
     3541 tgcacccggt ggagcgcctc ccaaaggtgt gggtggtgca ccttcatacg ctccaaaacg
     3601 gagtgcagtt cgtggtcacc ctcgggctct gagagcctcc gcaacgcctc ctctttggcc
     3661 gtgtggtcac cttcgcccac ggtcttctcc cttctttgac catcgtccgc aagtgctcgt
     3721 taggatacgg gccatctcca tcgcagcgcc tagtctgcga ggaacacacc gatcaggatc
     3781 gccatggcaa cgaccaggag cgcgatggcc aggtaagcca atcccctgga ccgttccgtg
     3841 ctcccgcttg cagtcagggc tccgaacgcg gcgaatccaa accaggccac ggcgttcagc
     3901 gtatcgcgat cgatgaccgc agcgatagcc ccgacgagcc acagcgccgt ggctatccac
     3961 ataaatgacc tgaaacgttg gctgcctgtt cccctacttc tgcccatctt ctaaccccct
     4021 tgtgacgcgc tggaacaggg taagtatttc acattccgtg ctctcgctct atttatcgaa
     4081 tggcccgagt tgctacccag ccgggatcag gagacgagaa tgtcggcaga gaacgaagcc
     4141 ctggttcgcc gctacgtcga ggaggtcttc gaccaatgca agctcgaggt catagacgag
     4201 ctcttcgctt ccgacttcac cctccacgac ccggaggttc aggaatcgtt tcgagggctg
     4261 cttgggtgat tccgggtggc tatctactgt agagcgaatc gatttctgaa ggactctttg
     4321 gccagggtga cgccctcggc ggtgagtggc acggacttgg ctttgctctt ggggtcgccg
     4381 atgaggccga ggtcgtggag acggtccatc gcatcccagt cgaaagtttt ccacgcacga
     4441 acctggccat ggtccttgaa agcgttgagg tacagcagcc ccagcacggc ttcatcaatc
     4501 tcttttgtct tgaggccgac ttctcggtct tccgagtcag gctcggagat ccacggcggt
     4561 cgggcaaaga acgtccgcag cttgctgaga atctcttcta gcttatattc cttgaccgtt
     4621 ccgaaccggc gcgcgatcaa ggagcggtgc atggtgaaga gcttgcccac cttgacaacg
     4681 ctccgcttgg gcagcctctc ctcgactatg tcctccgcgc ccagtgacgc ttcccattca
     4741 gagaacctct ctggaagccg ggatgtgatg gcgcagagga cgacgtcctc gtcgtcgaac
     4801 ccttcgggcg agatgaccag ggccgggcga cgcttgcggc ccgagaggtc ggtgaacggg
     4861 aagggcacca atactaccga gcctgtcggg taagcctcct ctgacaagcg agctagcctc
     4921 ttgcccgatc gacgggctct tcagcctcct cgtcccagac ctcgtcttcc tcattatccc
     4981 agaacgagaa ggagctctcg ctcgtggcca gccagcccaa cgactctcgc tccgctctgc
     5041 gggcgtcgat ctcgatggcc tcctcgatgg cgcgccggtc aagcggagag atcaccgcca
     5101 caggctctcc ccgcagggtc agtattaccc tctctccgtt gcggaccctc tcaacgatct
     5161 cgcccgtgtg ctgcttcagc tccctcgtcc ctacgctgta caagatcggt gctcccttcc
     5221 gagatggcgc ttcgccaacc gtgtcaagtg aacacaagtg tatcacctac cgtaccatgg
     5281 aatcggacgc cgggagaaac gtgggccacg agagcgtcga aggtagcgtc gatgcgtgct
     5341 cgaatctcct ctgcggtaga gctttagagc ggatgaaagg aaatcacatc gtggccttcc
     5401 taggtaagct cggggttgaa gctcgacgga cccgcggtgc agtcgaggat cttgccgagg
     5461 aggttgcccg gcgtcagggc gaacatcctg acgtattcgt ccttcgatcg cccctaggga
     5521 acgacctcgt ccagcttcgg gatggagctt tactcgctgc ggtcggtcgc gagtgcttta
     5581 tcgaggcggc tgaccttacg ggcagccctg ttcggatgga ttatgctttt gcccgcggcc
     5641 ttgttgtatt ccctctggga ctcgtcccgg atacggcggg cctcttcttc gtcccccgac
     5701 tcgacggccc tgtagaagtt ctttgaaagg ttgcgaaggc gggtcctgac gcccttgttg
     5761 tgggcgccac gcttgacgct ctgcttgtcg cgtttggatg gtgcaggcac actggctcct
     5821 cagatctgat acttatccga ccggatgaat gtaacacaag tagagcaaag gaacaatcat
     5881 ggtagaatct ccc
//
LOCUS       DATMJA010000012         7693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_119836, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000012 DATMJA010000000
VERSION     DATMJA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119836"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VNT12_00265"
     CDS             <1..581
                     /locus_tag="VNT12_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI44568.1"
                     /translation="MVEPMLDYVFEHGLKPAYIVDTHTHADHISGARELKSKSTAKIV
                     MHESAPASCVDMRVEDGDRLQVGDISIKVIHTPGHAKDLISVLLPDRILTADALLIGS
                     CGRTDLPNGNAVRQYHTLYYTYRALPEDLLVYPGHDYNGREHSILKEEKESNPKMLFD
                     TEEEFVEFMERENPGKLTPVYHLADALKANMA"
     gene            694..2142
                     /locus_tag="VNT12_00270"
     CDS             694..2142
                     /locus_tag="VNT12_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWI44569.1"
                     /translation="MSKKRTFTRRDFLRAAGAAGASLLGASGCAGFADRVPRLPEEYL
                     PGGGTGPNVILVIIDTLRKDHVGAYGNDWIQTPTLDALAEESLLFTQAHPEAMPTIPV
                     RRAIHTGMRTWPTRPPSFGWKPIPSGHTTVAEILSKEGFGTYLVTDTYVQFPMNFGRG
                     FGTYNMIRGQENDPYKDRSTISEKEMRDRYIILGEGKKALQYLANVQGRKGEEDWFAP
                     RVFLDAMDALEVAAGDTRPFFLVADCYDPHEPWDPPEEYVSLYDEGYKGKEPLDDNYG
                     SDDYLTDRQLLRMRALYAAEVTMMDRWLGKFIERTHELGVMEKTLFVVVSDHGHLLGE
                     HGYTGKLAYALYPELTDIVLMMRRPRGEGAGKSSDFYASTHDVAPTILGFLGVEQAEP
                     MDGQDLAPILEGKEPEQPRDHFTQGYKNYVCCRDEHRVMFCRTDMTDVHLFDAVNDTE
                     QRRDLAEAEPETVKRMYGEYAQKDAAGRLPNF"
     gene            2848..4800
                     /gene="sat"
                     /locus_tag="VNT12_00275"
     CDS             2848..4800
                     /gene="sat"
                     /locus_tag="VNT12_00275"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HWI44570.1"
                     /translation="MTRSKPVALWAVPRSISTAFERVFVEREDFEVLHEPFSASYYYG
                     EDRPSDRFADAEPKVEYSYERVLQDVFRPREQRVFLKDMAYHAKKVLSPEFASRFVNT
                     FIVRDPKYVLVSLYKMWPDFTLEETGYEELYRLFRYATDAGEDVAVVDAMTFSENPAG
                     VLAAYCEHLEVPFRSGSLTWESRDVRRWENWEGWHEDAESSTGIEPAERRDPELPEEL
                     EAAYEHCLPYYYELAAHAIPSVARRVGRTDGGPEDTDKEKILLRTETAIAPHGGELID
                     CVVPEEERRERSMEAADLPKVPLSLRALSDLQMISTGVFSPLEGFMLRDEYESVVEDM
                     RLKDGLAWSLPVTVSVDEEQAGGLAEGSEIALVDGTREPVATMVLRELYGYDKEREAR
                     MVYRTTDADHPGVAAVYRQGDVLLGGEVELLRPPDEGRFPRYYYTPAELRASFAEKGW
                     KRIVGFQTRNPVHRAHEYIQKSALETVDGLLLNPLVGETKSDDIPADVRMRSYETILD
                     RYYPEDRTMLAVFPAAMRYAGPREAIFHAICRKNYGCSHFIVGRDHAGVGSYYGTYDA
                     QHIFEEFEPEELGITPLFFEHAFFCTECEGMGSSKTCPHDPDSHVFLSGTKVREMLSK
                     GEYPPPEFSRPEVIEVLMQGQRVESR"
     gene            complement(4856..5392)
                     /locus_tag="VNT12_00280"
     CDS             complement(4856..5392)
                     /locus_tag="VNT12_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWI44571.1"
                     /translation="MNGIAFRLRAHIGGLPYVILYPVPAIAFLPLLILWFDTSPTSLV
                     VVTVCSVTLPSAINLSAGLKSVNPTIMLVGQNLGLRGWGMFSAVLLPAALPHAISGLR
                     AGWASGWRTIIAAGLVFGVAGGGPTFFTNDAGDFLPTPELLAGLLTIAFAGILVEATF
                     RLLERGTVARWGMKVGRE"
     gene            complement(5495..5806)
                     /locus_tag="VNT12_00285"
     CDS             complement(5495..5806)
                     /locus_tag="VNT12_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44572.1"
                     /translation="MTSLLFRADAKGQAHGMDGAARGRFGPKLGSVVLASLLLCEVAA
                     AMLGAEAVFFSTLVAAWLVLPAVVGPRKVLDAGCFYMLISLGIVVVVDVARYGPIALL
                     A"
     gene            complement(5803..5991)
                     /locus_tag="VNT12_00290"
     CDS             complement(5803..5991)
                     /locus_tag="VNT12_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44573.1"
                     /translation="MMRVEVGKMKWDLDLWYELWERAGSQGLDTWYDLRGEARDRPDT
                     GHAPDAGDDIIEDGREGT"
     gene            6206..7096
                     /locus_tag="VNT12_00295"
     CDS             6206..7096
                     /locus_tag="VNT12_00295"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HWI44574.1"
                     /translation="MGARSGLAGPALSYLICATPRSGSTLLCAALDDTGIAGHPEEHF
                     EVLLETGQPRQPRDYFQRSNDPEVWALLDDPEFRDVLGEYGGRYSEHPARRDPLWRPP
                     DFERLAEGALHKGTTENGVLGTKIMWAYFRDFVRLARRTTAPDVRPCEVPASVLPNLR
                     RFVWMRRRDTTRQAVSLWKALQTKQWRRDSDEDVSGQGLRFSFPAVDHLKLRIDEHNA
                     AWQDFFEGCGVEPMEVVYEELVEDYRGTVLWLLDGIGISVPENFAVAEPKMRRQADEL
                     SEKWVRLYDEHAAAKTVQKG"
     gene            7099..>7693
                     /locus_tag="VNT12_00300"
     CDS             7099..>7693
                     /locus_tag="VNT12_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWI44575.1"
                     /translation="MPDIPNILYIHSHDTGRWVRPYGYSVRTPNIQRLAEEGVLFRKA
                     FCAAPTCSGSRACLLTGQYAHSNGMVGLAHRGFSLKDYRQHIVHTLRDLGYWSALIGE
                     QHISKKPDIIGYDEVIKISTSHTDDVVPVTLDLLERASDRPFFLSVGFFETHREWFRP
                     SSPKKANYVMPPPNLPDAPETRLDMAAFQESARSLDRG"
BASE COUNT         1579 a         2315 c         2420 g         1379 t
ORIGIN      
        1 agatggtcga gccgatgctc gactacgtct tcgagcacgg cctcaagccg gcctatatag
       61 tggacaccca cacccacgcc gaccacatct ccggggccag ggagctcaag tccaagtcga
      121 cggcgaagat cgtgatgcac gagagcgccc cggcgagctg cgtagacatg cgggtcgagg
      181 acggtgaccg cctccaggtc ggggacattt ccatcaaggt catccacacc ccggggcatg
      241 ccaaggacct gatctccgtt ctactccccg accgtatcct gaccgccgac gccctgctca
      301 tagggtcctg cgggcgcacc gacctcccca acggcaacgc ggtccgccag taccacacgc
      361 tctattacac ttaccgcgcc ctccccgaag atctgctcgt ctacccggga cacgactaca
      421 acggccgcga gcactccatc ctgaaggagg agaaggagag caatcccaag atgctcttcg
      481 acacggaaga ggagttcgtc gagtttatgg aaagggagaa cccgggcaaa ctcacgcccg
      541 tttatcatct ggccgacgcc ctcaaggcaa acatggcctg agccagatca gtcaccaggt
      601 ggtctctcca cttcgtccgg tcggtgatga tggacgacgt gttagctgct agagtgcggc
      661 ttttgggggt aacggttcgg gagtcgggct ggagtgagca agaagaggac gtttacgcgc
      721 agggacttct tgagagccgc cggcgcggcc ggggcgtccc tgctcggcgc gtcggggtgc
      781 gcaggcttcg ccgacagagt tccgcggttg ccagaggagt accttcctgg tggtgggacc
      841 gggccgaacg tcatcctggt gatcatagac accctgagga aggatcacgt cggtgcctac
      901 ggcaacgact ggatacagac ccctaccctg gatgccctcg ccgaagagag cctgctcttc
      961 acccaggccc atcccgaggc catgcccacg ataccggtga ggcgtgcgat acatacaggg
     1021 atgaggacct ggccaacccg accgccctcc ttcgggtgga agccgatccc ctccggacat
     1081 accacggtgg cggagatact gtcgaaagag ggcttcggca cctacctcgt gaccgacacc
     1141 tacgtacagt tccccatgaa cttcggccgg ggattcggga cttacaacat gatccggggc
     1201 caggagaatg acccttacaa ggaccgctcc acgatctccg agaaggagat gcgggaccgt
     1261 tacatcatcc tcggggaggg caagaaggcc ctccagtacc tggccaacgt acagggccgc
     1321 aagggcgagg aggactggtt cgcccccagg gtgttcctag acgccatgga tgcgctcgag
     1381 gtggccgcgg gggatacccg gcctttcttc ctggtggcag actgctacga tccccacgag
     1441 ccctgggacc cgccagagga gtacgtgagc ctctacgacg agggctacaa aggcaaggag
     1501 ccccttgacg ataactacgg cagcgacgac tatctcaccg accgccaact cctcaggatg
     1561 agggcactct acgccgctga ggtcaccatg atggaccgct ggctcgggaa gttcatcgag
     1621 aggacgcacg agctcggggt aatggagaag accctgttcg tggtcgtctc cgaccacggc
     1681 catctccttg gagagcacgg ctacacaggg aagctggcct acgccctcta ccctgagctg
     1741 acagacatag tcctcatgat gaggcgcccc cggggcgagg gtgctggcaa gagcagcgac
     1801 ttctacgctt ctacccacga cgtcgcgcca acgatccttg gtttcctagg ggttgagcag
     1861 gccgaaccca tggacggtca ggacctggct cccatcctgg agggtaagga gccagagcaa
     1921 ccgagggacc atttcaccca aggctacaag aactacgtgt gctgcaggga cgagcacagg
     1981 gtaatgttct gccgcaccga catgaccgac gtccacctct tcgacgccgt aaacgacaca
     2041 gagcagaggc gggacctggc cgaagccgag cccgagacgg tcaagaggat gtacggagag
     2101 tacgcgcaga aagatgccgc ggggcggctg ccgaacttct gatagggtcc atcactaggg
     2161 gccggggcat aatgctccgg cctttctctt gctcctcaaa cgtcggcttg ggcatagtct
     2221 aggaaccgta ggatggtcta tttggggtat gcgcttgcta tcacaaggga cgtatagtgg
     2281 gatccttaca cgagcgggac ccggaggagg gcttcgcacc atcgatcgtc aaagtggtgg
     2341 gccttcttct gttggcggtg tgggcgttcg ttatggcggc gttcatgtgg cgcaacggta
     2401 gtcgccgacg catcgcccgt cttggatcga cttccacagg accggcgcag cgccccgccg
     2461 gcacacgctg accctgcgaa cccggcatcg cagccgggat agtcaacctc gagttgggag
     2521 agaacggcag tctgtactac ctggcaagga gttcgaactc agccccagcg tcggtgcgcg
     2581 agatcaggtc atgggaacga tccatcctaa gcgcccaggg tcaaaacggc ccacaaatag
     2641 aacctccaag agaaaaatcg gcggggtcgc ttgacctcga aatgatgctg gtctaaagtt
     2701 ttccgatgct cacaaatcta gtaggcaaaa tggttaattg gcctggtggc acgctctaat
     2761 gtcattacgg catttcccgg tatcatgcgt gggagacctg ggaggacccg ggacgcttac
     2821 cctgttgagc acgagagaga ggcgcatgtg acacggagca aacctgttgc gttgtgggct
     2881 gtaccacgtt cgatatctac ggcgttcgag cgggtctttg tggagaggga ggatttcgag
     2941 gtactacacg agccgttttc ggcctcttac tactatggag aggaccgccc gagcgaccgc
     3001 tttgcggacg ccgagccgaa ggtcgagtac agttacgagc gggtgctcca ggacgtcttc
     3061 aggccacgcg agcagcgcgt tttcctcaag gacatggcct accacgcaaa gaaggtgttg
     3121 agccccgagt tcgcctcgcg gttcgtcaat acgtttatcg tgagggaccc gaagtacgtg
     3181 ttggtgtcgc tgtacaagat gtggcccgac tttacgttgg aggagaccgg ctacgaggag
     3241 ttgtacaggc tcttccgtta tgcgacggac gcgggggagg acgttgccgt cgtcgacgcg
     3301 atgaccttct cggagaaccc tgcgggcgtg ctcgccgcct actgcgagca cctcgaggta
     3361 cctttcaggt ccggctccct cacgtgggag tcgagagatg ttcggagatg ggagaactgg
     3421 gaggggtggc acgaggatgc cgagagcagc actggcatag agccggccga gcgcagggac
     3481 cccgagctgc ccgaggagct cgaggccgcc tacgagcact gtttgcccta ctactacgag
     3541 ctggcggcgc atgcgatccc atccgtagcg cggcgagtgg gacgtaccga cggaggtcct
     3601 gaggacacag acaaggagaa gatcttgctg agaacagaga ctgcgatagc cccgcatggg
     3661 ggagaactca tagactgcgt agtccccgag gaggagcgca gggagcgttc gatggaggct
     3721 gcggatctgc caaaggtgcc cttgagcctg cgggccctat ccgacctgca gatgatctca
     3781 accggggtct tcagccccct cgaagggttc atgctgcgcg acgagtacga gagcgtggta
     3841 gaggatatgc gcctgaaaga cggcctggcc tggagcctgc ccgtcaccgt atccgtcgac
     3901 gaggagcagg ccggagggct cgcggagggc tccgagatag ccctggttga cggtactagg
     3961 gagcccgtgg ccaccatggt gctcagggag ctctacggct acgacaagga gcgcgaggcc
     4021 aggatggtct acaggacgac cgacgcggac catccaggtg tagcggcggt ctacaggcag
     4081 ggcgacgtcc tgcttggcgg cgaggtcgag ctcctgcgtc cgcccgatga ggggcgtttt
     4141 ccccgctact actacacgcc ggcggagctc agggcctcgt tcgccgagaa gggatggaag
     4201 cggatcgtcg gcttccagac caggaacccc gtacaccggg cgcacgagta catccagaag
     4261 agcgccctcg agaccgtgga cgggctcctc ttgaatccgc tcgtcgggga gaccaaatct
     4321 gacgacatac cggccgacgt caggatgcgc tcctacgaga cgatcctgga tcgctactat
     4381 cccgaagacc gcacgatgct cgccgtcttc cccgccgcga tgcgctacgc cggtccgagg
     4441 gaggccatct tccacgccat ttgtcgcaag aactacggtt gctcgcactt catcgtaggc
     4501 cgcgaccacg ccggcgtcgg aagctactac gggacctacg acgcccaaca catcttcgag
     4561 gagttcgagc cggaggagct ggggatcaca cccctcttct tcgagcacgc gttcttctgc
     4621 acggagtgcg agggtatggg ctcatcgaag acttgcccgc acgacccaga ctcccacgtc
     4681 ttcctctcgg ggacgaaggt gcgcgagatg ctctccaaag gcgagtaccc gcccccagag
     4741 ttctcccgcc ccgaagtgat cgaagtgctc atgcaaggtc agcgggtcga gagcagatag
     4801 caatcggctt tcagcctttt gcttgttcac agctgcttac gaatcctgtt gatcactact
     4861 ccctgccaac cttcattccc catcgggcga cggtaccacg ctccagaagg cggaaggtcg
     4921 cttcgacgag gatgccggcg aaggcgatgg taagtagtcc ggcgaggagc tccggtgtcg
     4981 gcaggaagtc gccggcgtcg ttggtgaaga acgtggggcc cccgcctgcc acgccgaaga
     5041 cgagtccggc ggcgatgatg gtgcgccagc cggatgccca gcctgctctg agtccggaga
     5101 tggcgtgcgg caaagctgcg ggtaggagca ccgcgctgaa catcccccag cctcgcagac
     5161 ccaggttctg cccgaccagc atgatcgtgg ggttgacgct cttcaacccg gcgctcaggt
     5221 tgatggcgga gggcaaggtg accgagcaga cggtaaccac gaccagtgaa gtgggactcg
     5281 tgtcgaacca gaggatgagg agcggcaaga aggctatggc gggtacggga tagagaatga
     5341 cgtatgggag gcctccaatg tgtgcccgca gcctgaacgc gatcccgttc aaagggagtc
     5401 gttatgaatc aggtgaagga gcgtgcaact cgcgaagtgg ctgataagga tagcgtggac
     5461 gttggatcgg ctagggggtg gctccgctgt ggtgttacgc taggagagcg atcgggccat
     5521 accgggcgac atcgaccacg accacgatac cgagcgagat gagcatatag aagcatccgg
     5581 cgtcgaggac tttccttggt cccacaacgg ccggtaggac cagccaggct gccacgaggg
     5641 tcgagaagaa aaccgcctcg gctcccagca tcgcggctgc cacctcgcac aagagcagcg
     5701 aagccaacac gaccgacccg agcttgggcc cgaaccgtcc gcgggccgca ccatccatac
     5761 cgtgcgcctg gcccttcgca tcagctctga acaagagact ggtcaagtcc cctctcgccc
     5821 gtcttcgata atgtcgtctc cggcatccgg cgcgtgtccg gtgtcggggc gatcgcgcgc
     5881 ctcaccacgc aggtcgtacc aggtatccaa tccctgactg cccgccctct cccaaagctc
     5941 gtaccagaga tccagatccc acttcatctt ccctacctcc actctcatca tctgttgtct
     6001 actttataag tagacattat ggtatacaac tgttccatcg gagtcaagtg ccggggcctc
     6061 atttggcgca catccgtggg caggagacaa gatcgtctca ggcatccagg agtgttgatc
     6121 cactgacgct ttcatgtatt ctttaccatc gtaatagaca aagtagtttt agtagatgtt
     6181 gatcaggtaa tcgggaggaa ggtttatggg tgctagatcc gggctcgctg ggccggcgct
     6241 gtcgtatttg atctgtgcga cgccgcgcag cgggagtaca ttgctttgcg cggcgctcga
     6301 tgacacgggg atagcgggtc atcccgaaga gcatttcgag gtcttgctcg agacggggca
     6361 gcccaggcaa cctcgcgact acttccagcg ctccaacgat cctgaagtgt gggcgctcct
     6421 cgacgatcca gagttcaggg acgttctggg cgagtatggt ggcaggtact ccgagcatcc
     6481 ggcgcgcagg gacccgcttt ggcgcccgcc cgacttcgag aggctggcag aaggggcgct
     6541 gcacaaaggc actaccgaaa acggcgtgct cgggaccaag atcatgtggg cttatttcag
     6601 ggacttcgtc cgactcgcgc gccggacgac tgcgcctgac gttcgaccct gcgaggtgcc
     6661 cgcctccgtc ttgcccaacc tgcggcggtt cgtctggatg cgccgcagag acaccacccg
     6721 ccaagccgtc tcgctgtgga aggcgctcca gactaagcag tggcggcgag actcggacga
     6781 ggatgtaagt ggacagggcc tgcgtttcag ctttcccgcc gtagaccact tgaagctacg
     6841 tatcgacgag cacaacgccg cctggcagga tttcttcgag gggtgtggcg tggaacctat
     6901 ggaggtggtc tacgaggagc tcgtcgagga ttaccgggga acggtgttat ggcttctcga
     6961 tggcatcggg atctctgttc cggagaattt tgccgtcgca gagcctaaaa tgaggcggca
     7021 ggctgacgag ctctccgaga agtgggtccg cctgtacgac gagcacgctg ccgcgaaaac
     7081 cgtgcagaaa ggatagagtt gccggatatt ccgaacattc tctacataca ctcccacgac
     7141 accgggcggt gggtcaggcc ttacggctac tctgtaagga cgcccaatat ccagcggctc
     7201 gccgaggagg gcgtgctctt ccgcaaggca ttctgcgctg cccctacgtg ttcggggagc
     7261 cgggcatgtt tgctgaccgg gcagtacgcc cactcgaacg ggatggtagg cctcgcccac
     7321 cgcgggttct cgctcaagga ttacaggcag cacatcgttc acacgctgcg cgatctcggg
     7381 tattggtcgg cgctcatcgg cgagcagcac atctccaaga agcccgacat catcggctac
     7441 gacgaggtga tcaagatcag caccagccac accgacgacg tcgtacccgt gacgctagac
     7501 ctgctggaac gcgccagcga cagacccttt ttcctctccg tcgggttctt cgagacccac
     7561 cgcgagtggt ttcgtccgag ttcaccgaag aaagccaact acgtcatgcc accgcccaac
     7621 ttgccggacg cgccagagac gcgcctggac atggccgcct tccaggagag cgcccgctcg
     7681 ctcgacaggg gca
//
LOCUS       DATMJA010000013         5144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_297717, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000013 DATMJA010000000
VERSION     DATMJA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297717"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..2375
                     /gene="hypF"
                     /locus_tag="VNT12_00305"
     CDS             51..2375
                     /gene="hypF"
                     /locus_tag="VNT12_00305"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HWI44576.1"
                     /translation="MRAPIQERWEVSVRGIVQGVGFRPFVYALALRHGLAGLVRNDAE
                     GVHVEVEGDAERLDLFLRGIEEEAPPLAVVEAVSWRPLAALGERGFRIEESREGFRRR
                     ALISPDVATCEECLREVFDPGDRRYRYPFTNCTNCGPRFTITRAVPYDRDTTTMSRFE
                     MCPECRREYDDPTDRRFHAQPNACPTCGPRVRLLDKFGHRLHAKPDDPILRTAKMLRG
                     RAVVAIKGLGGYHLACDPFDEKAVRTLRGRKVRQDKPFALMARDLAQVHEICHVSPGE
                     EALLTSPARPIVLLERRENAGVAEGVAPRQSTLGVMLAYTPLHHLLLRDAGIPLVMTS
                     GNNSDEPIAYRDEEALDQLCEIADYFLVHDREIHMRCDDTVVRQARGGPYPVRRSRGY
                     APAPLRLVANVTRHTLACGGELKNTFCLARDRHAFMSHHIGDMENYETLQSFREGVKH
                     YSRLFDVSPELVAYDLHPEYLSTKYARELEETGLPAVGVQHHHAHVASCLADNERPAE
                     ERVIGVTLDGTGYGTDGAVWGAEFFEGSLNRGFTRRAHLEYAPLPGGSAAIRQPWRMA
                     LAQLISFYGEEDTLKLPLAAVQQAGERSVMLIARLVERKMNTPPTSSAGRLFDAVAAL
                     TGVPGTERTTYEGQAAVELELAADGPASRGYPFRLRSKDQGWIVETGEIIRGVVEDLL
                     AGRATGEISSRFHRTMAEVVAAGCEKIRGAGGTTAVALSGGTFQNMLLLGQVIELLQG
                     KGFQVYRHRRVPANDGGLALGQAILADRTFREEA"
     gene            2387..2665
                     /locus_tag="VNT12_00310"
     CDS             2387..2665
                     /locus_tag="VNT12_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HWI44577.1"
                     /translation="MCLGIPGRIVEIIDEDLLLAKAEVSGVRRTINIGLVHNEDEQIE
                     VGDWVLIHVGFAMSKLNETEAQSQLQALEDIGEAYEQELAELRASQIE"
     gene            2668..2922
                     /locus_tag="VNT12_00315"
     CDS             2668..2922
                     /locus_tag="VNT12_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HWI44578.1"
                     /translation="MERETKLPDSYCTLDHDGCIVCSDAGIPLQVVSIEGDDALCEDA
                     AGNRTEIAVDLVAPVKAGEVLLTHGGVAIAKVQKADKLKC"
     gene            2975..4102
                     /gene="hypD"
                     /locus_tag="VNT12_00320"
     CDS             2975..4102
                     /gene="hypD"
                     /locus_tag="VNT12_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HWI44579.1"
                     /translation="MKYVDEFRNAEIARALSAEIAAITDPTKHYKLMEVCGGHTHTIY
                     RHGIKDLLPDQIELVHGPGCPVCVLPMGRVDDGVAVAERPEVIFTCFGDMMRVPGSKG
                     NLLEVKAEGADIRMVYSPLDALRVARENPDREVVFFAIGFETTAPSTALTLQRAKKEG
                     LENFSVFCNHVTIVPPVRALLDSPDLRLDAFIGPGHVSTVIGCRPYEFMPEDYGKPCV
                     VAGFEPLDLLQSILMLMRQLGEGRCEVENQYSRIVPWEGNLAALKSMAEVFELRPYFE
                     WRGMGFIAQSALKLSDAYADFDAELRYSVPGVRVADPKACQCGEVLKGVLKPWECKVF
                     GTACTPEHPLGTLMVSSEGACAAYYNFGRKSFEKVEIGTRQ"
     gene            4099..>5144
                     /gene="hypE"
                     /locus_tag="VNT12_00325"
     CDS             4099..>5144
                     /gene="hypE"
                     /locus_tag="VNT12_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HWI44580.1"
                     /translation="MTEREQHVLDTLEQNRRRPPRFTSQRIDMSHGAGGKATHKLIEG
                     LLLPALENPALAPLADAATLPFGDAQIALTTDSFVVRPLVFPGGSIGELAVNGTVNDL
                     AVSGATPIGLSAALIIEEGLETEVLAYEIEAMASSAENAGVAVATGDTKVVERGKGDG
                     LYVVTTGVGLADQELNLSTASVRPGDKILCSGTLGDHGAAILIARGDLELEAEVLSDT
                     RPLTPLAEALKNSARQGLRFMRDPTRGGVGTVLNELARDAGHGVALWEEKLPIRDVVH
                     GACEILGIDSLYVANEGKLLAVVAPEQADAALEALRNTEGGEEASIIGEVREEPARMV
                     VMHTGFGGTRMIDML"
BASE COUNT         1028 a         1676 c         1614 g          826 t
ORIGIN      
        1 gtatctgaat ataagggaca tgtagtagtc gtggaacgcg aactggcgcc atgagagcgc
       61 cgatacagga acgatgggag gtatcggtcc ggggcatcgt gcagggcgtg gggttccggc
      121 ccttcgtcta cgcactggcg ctacggcacg ggcttgccgg gctggtccgc aacgacgccg
      181 agggagtcca cgtcgaggtg gagggcgacg ccgaaaggtt ggacctcttc ctgcgcggga
      241 tagaggaaga agccccgccg ctcgcagtcg tggaggccgt ttcctggcgt ccgctcgcag
      301 cgctgggcga gcggggtttc cggatcgagg agagcaggga gggcttccgg cggcgggccc
      361 tcatatctcc ggatgtcgcc acctgcgaag agtgcctgcg ggaggtcttc gacccagggg
      421 atcgccgcta ccgctacccg ttcaccaact gcaccaactg tggtccccgc ttcaccataa
      481 cccgcgcggt tccttacgac cgggacacga ccacgatgtc ccgcttcgag atgtgcccgg
      541 agtgccggcg tgagtacgac gatcccaccg accgccgctt tcacgcccag ccaaacgcct
      601 gccctacctg tggaccacgg gtacggttgc tggataagtt cggccaccgg ttgcacgcaa
      661 aaccggacga ccccatcctg cgaacggcca agatgctgcg cggcagggcc gtcgtcgcca
      721 taaagggcct cggcggctat cacctggcct gcgacccgtt cgacgagaag gccgtgagga
      781 ccctacgagg ccgcaaggtg cgccaggaca agcccttcgc gctcatggct agggatctcg
      841 ctcaagtgca tgagatctgc cacgtctctc ctggagaaga ggcgctgctc acctcgccgg
      901 cccgtccgat agtgctgctg gaacgcaggg agaatgccgg cgtcgccgaa ggagtagccc
      961 cgcgccagag cactctaggc gtaatgctcg cctacacgcc gctgcaccac ctgctcctcc
     1021 gcgacgcggg catcccgctc gtcatgacca gcggcaacaa ctcggacgag cccatcgcat
     1081 atcgggacga ggaagcgttg gaccagctgt gcgagatcgc cgactacttc ctggtccacg
     1141 accgggagat ccacatgcgc tgcgacgaca ccgtggttcg gcaagccaga ggaggacctt
     1201 acccggttcg tcgctcccgg ggttacgccc cggctcccct ccggctggtc gcaaacgtca
     1261 ccaggcacac gttggcctgt ggtggagagc tcaagaatac attctgcctg gccagagacc
     1321 gccatgcctt catgagccac cacatcgggg acatggagaa ctacgagacg ctgcagtcgt
     1381 tcagggaagg agtcaagcac tactcaaggc tcttcgacgt tagtccggaa ctggtcgcct
     1441 acgacctcca tcccgagtac ctgtccacca agtacgcccg tgagctcgag gaaaccggac
     1501 tccctgctgt cggcgtccag caccaccacg cgcacgtcgc aagctgcctc gctgacaacg
     1561 aacgtcctgc agaagagcgc gtcatcgggg tcaccctcga cgggaccggc tacggcacgg
     1621 acggcgccgt ctggggcgca gagttcttcg agggaagcct gaatagaggg ttcactcgcc
     1681 gggcgcatct cgaatacgcg ccgctccctg gaggatcggc ggccatccgc cagccgtggc
     1741 gtatggcact ggcgcaactc atcagcttct atggagaaga agacaccctg aagctcccgc
     1801 tcgccgccgt gcagcaagcc ggcgaacgga gtgtaatgct catcgcccgt ctcgtggagc
     1861 gcaaaatgaa caccccgccc acctccagcg cgggtcggct ctttgacgcc gttgcggccc
     1921 tcaccggggt gcccggcacg gagcgaacta cctacgaggg acaggccgcg gtggaactgg
     1981 aactggccgc tgacggcccg gcaagccggg gctatccctt ccggctgcgg tccaaggacc
     2041 aaggatggat cgtggagacc ggggagatca ttcgcggggt cgtggaggac ctcctggcgg
     2101 gaagagcgac gggagagata tcttccaggt tccaccgcac gatggccgag gtggtcgccg
     2161 caggttgcga aaagatccgg ggagccgggg gaacgaccgc cgtcgcgctc tccggcggga
     2221 cttttcagaa catgctcctt ctgggacagg tgatagagtt actgcaaggg aaaggcttcc
     2281 aagtctaccg gcaccgccgc gtgccggcca acgacggggg actcgcattg ggacaggcga
     2341 tactggcgga caggactttc agagaagaag cgtagggagg cataacatgt gtctggggat
     2401 accgggacgg atcgtggaga tcatagacga agaccttctg ctcgcgaaag ctgaagttag
     2461 cggggtcagg cgcaccatca acatagggct cgtccacaac gaggatgagc agatcgaagt
     2521 cggggactgg gtcttgatcc acgtcggctt cgctatgtcc aaacttaacg agacggaggc
     2581 gcagtcccag ctacaggccc tggaagacat cggcgaggcc tacgagcagg agctggccga
     2641 gctacgcgcc tcccagatag agtagccgtg gagcgagaaa caaagctgcc tgactcctac
     2701 tgcaccctcg accacgacgg ctgcatcgtc tgctccgacg ccggcatccc gttgcaggtc
     2761 gtctccatcg agggcgacga tgccctctgc gaagacgccg caggcaaccg caccgagatc
     2821 gcagtggacc tagtcgcccc cgtaaaggcg ggagaagtcc tcctaacgca cggaggcgtc
     2881 gccatagcca aagtccaaaa agctgacaag ctgaaatgct gacaagcctg cgaagcaggc
     2941 gtgctgaaat gctgccgacc gaagggaggc cctcatgaaa tacgtagacg agttcagaaa
     3001 cgccgagatc gcccgcgcct tatctgccga gatagccgcg atcacagacc ccacaaaaca
     3061 ctacaagctg atggaagtct gcggcggcca cacccacacg atttaccgcc acggcataaa
     3121 ggacctctta cctgaccaga tagagctggt ccacggcccc ggctgcccgg tctgcgtcct
     3181 cccgatgggc agggtggacg acggtgtagc cgtcgccgag cgccccgagg tcatcttcac
     3241 ctgcttcggc gacatgatgc gggtcccagg ctccaaggga aacctgctcg aggtaaaagc
     3301 cgaaggcgcg gacatccgca tggtctactc gccgctcgac gccctgcgcg ttgcgcgcga
     3361 gaaccccgac cgcgaggtgg tcttcttcgc catcggcttc gagaccaccg ccccatcaac
     3421 ggcgctgacc ctgcaaaggg cgaagaagga gggcctggag aacttctccg tgttctgcaa
     3481 ccacgtcaca atcgtcccgc cggtgcgggc gctactcgac tctccggacc tgcgcctcga
     3541 tgcctttatc gggccaggac acgtctctac ggtcatcggc tgcaggccat acgagttcat
     3601 gcccgaggac tacggcaagc cttgcgtggt cgcaggtttc gagccgctcg acctcttgca
     3661 gtccatcctc atgctcatgc gccagctcgg cgaggggcgc tgcgaggtcg agaaccagta
     3721 cagcaggatc gtcccctggg aaggcaacct cgcggcactg aagagcatgg ccgaggtctt
     3781 cgaactccgt ccgtacttcg agtggcgggg gatgggcttt atagcccagt cagccctcaa
     3841 gctctcggac gcctacgcgg acttcgacgc cgagctgcgt tactctgtac ctggcgtgcg
     3901 cgtcgccgac cctaaggcct gccagtgcgg cgaggtactc aagggtgtgc tcaagccctg
     3961 ggagtgcaag gtcttcggca cggcctgcac gcccgagcat ccgctcggca cgctcatggt
     4021 ctcatccgag ggagcttgcg ccgcctacta caacttcggg cgcaagtcgt tcgagaaggt
     4081 cgagatcggg accagacaat gactgagaga gagcagcacg tcctcgacac gctggaacag
     4141 aaccgccgca ggccgccgag gttcacatcc caacgcatag acatgagcca cggcgccggg
     4201 ggcaaggcta cccacaagct catcgagggt ctgctcctgc ccgccctcga aaacccggcg
     4261 ctcgcccctc tcgccgacgc ggcgacgctc cccttcggag atgcccagat agccctcacc
     4321 accgactcct ttgtcgtgcg ccccctggtc ttccccggcg gctcgatagg agagcttgcg
     4381 gtaaacggca ccgtcaacga cctcgccgtc tctggcgcca cccccatagg cctctccgcc
     4441 gccctcatca tcgaagaggg actcgagacc gaggtgctcg cctacgagat tgaagccatg
     4501 gcgagctcag ccgagaacgc aggggtcgct gtcgccacag gagataccaa agtggtggag
     4561 cgtggcaaag gcgacggact ctacgtggtt actacggggg tggggctcgc cgaccaggag
     4621 ctgaacctct ctacagcgtc cgtccgtccg ggagataaga tcctctgttc cggtactctg
     4681 ggcgaccatg gagcagccat cctgatcgca cgcggagacc tcgagctcga agccgaagtc
     4741 ctttccgaca caaggcccct cactccgctc gcggaggctc tcaaaaactc cgctagacag
     4801 ggcctgcgct tcatgcgcga ccccacgcgt ggcggcgtcg ggaccgtgtt gaacgagcta
     4861 gcccgcgatg cgggccatgg cgtcgccctc tgggaggaga agctccccat cagggacgtc
     4921 gtccatggag cgtgtgagat cctgggcata gactccctct acgtcgcaaa cgaaggcaag
     4981 ctcctcgccg tggtagctcc agaacaagcg gacgccgccc tcgaagcact acgtaatacc
     5041 gaaggcgggg aagaagcgtc tatcatcggc gaggtccgcg aggagccggc gcgcatggtc
     5101 gtcatgcaca cagggttcgg cggcacccgc atgatcgaca tgct
//
LOCUS       DATMJA010000014         5474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_831217, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000014 DATMJA010000000
VERSION     DATMJA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831217"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VNT12_00330"
     CDS             complement(<1..678)
                     /locus_tag="VNT12_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HWI44581.1"
                     /translation="MKHSLEIALRYGVAMLAVGAALGIKLLLDPLTVQDTPFLLVFSA
                     IIVSAWYGGLGPGLLATAISALATDYFFLNPRGTLTGFSVEGIDVLAFMLEGVLVSVL
                     TSSLRSARDRAQRSTLEARSHQESLRESEERFRLMVEGVKDYAIFMLDPGGCVTTWNE
                     GAERIEGYGEEEILGRHFSSFYREEDVERGHPEDVLRAALAEGRYVEEGLKVRQDGSR
                     FWASAVIT"
     gene            complement(902..1228)
                     /locus_tag="VNT12_00335"
     CDS             complement(902..1228)
                     /locus_tag="VNT12_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44582.1"
                     /translation="MVPGRQERLAGLFVVVGAAIVFAPAIWLLQRWSEAVARDPADAW
                     GARWLGEHLTTHPYLTAGAVLISIVLSSGMVMLILVSLAKRLAARLTDRGRPPWSGSR
                     EKDSGA"
     gene            1545..4379
                     /locus_tag="VNT12_00340"
     CDS             1545..4379
                     /locus_tag="VNT12_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI44583.1"
                     /translation="MTILVGCLVLVGWTFAFGDFRGVLPGPVAMNPVSAVGLLLAGVS
                     AWLLQDERAGGRVRWISRVCALVVALVGLSRLIQVRFGLEPGIDQILFPERLASGAAS
                     TGFPNRMAFDTALDFVLIGGALLFLDRRTRRGLWVSQYLALTVFVFSVLAFAGYVYGV
                     ANLYANTSYIPIASMTFVVLSAGVLCARPERGLMVVVTSRNVGGVVARRLLPAAILVP
                     LVLGWFRLRGQQVGLYGTELGVSLFTVANMFVVAALVWWSARLLYRIDGERKLAEEEM
                     QAARERAEEANRAKSEFLANMSHEIRTPMNGIIGMTDLLLDSPLDEEQREYAETVHYS
                     GQTLLAILNDILDFSKIEAGSLNLEAINFELRREVEEVAALLAGHAHEKGLELVSFVE
                     PGTPTAVRGDPFRLRQVLTNILGNAIKFTEEGEVTLRADLVEDTPDTATVYFEVSDTG
                     IGMTEEQRSRLFNPFTQADASTTRRYGGTGLGLAISKQLVLMMGGEIGVRSEPGVGST
                     FWFTTRLEKRPEDRRATPVPRAGLRDLRVLIVDDHETNRRILHMQITSWGMRDDMAEN
                     GAEALDLLLAAAESGEAYDLAVLDMQMPDMDGIQLASAISAEPAISSTRLVLLTSIGI
                     NINETARMAGVEVVLNKPVRQSQLHDALATMLGTPTEAQARPSRRGVRPAPHATPAAE
                     EQASRGYVLLAEDYPVNQMVAIRMLERSGYQVDAVNNGREAVEALSNIQYGAVLMDVQ
                     MPEMDGYEATAEIRRREGSLRHTPVIAMTADAMQGDRERALAAGMDDYIAKPVKHEEL
                     EAVLECWIPHPEQEPLAQRGDGAPGANEDTPALDLSVLESRRGPQRDGEPDKLARIVG
                     LFIDDVPLRLDELRKAVERGEAQKVEETAHMLKGGSGYMGAVQMSEICAGIQGLGASG
                     ELWRAPELLDELEAEFKRIRPALEAAVTAN"
     gene            4393..5223
                     /locus_tag="VNT12_00345"
     CDS             4393..5223
                     /locus_tag="VNT12_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44584.1"
                     /translation="MSTGAKSLSTLAVALTLFGLAGAAVLATVPPQFMGPERPGFAVF
                     LVAGLTVIVLTAGFFGYIYRLGMGFGRTALVLAAGYNALIAAVKLGLAPAALYQANRE
                     QSFDASMGDPNDLLFYLGVGSGVLLLYLLVFGVMYSVFRLRFRRRALPSEPPPEQRGR
                     WSNRTIVVGVVVLVAFAASFLWIMPVVYVGLPTLSYLLYIFTTFGSAITLALILAAVL
                     AYKSFDEVEKRAVRLGDATLLASFFWLGLTLIVLYHVMWVIFLLTLVSIWPFRTYTPK
                     "
     gene            5232..>5474
                     /locus_tag="VNT12_00350"
     CDS             5232..>5474
                     /locus_tag="VNT12_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44585.1"
                     /translation="MLALYWSLLRYRVAVMIILFMRLGAAWHGGLTAIDAPVILTILA
                     LASSYVSATSVNDISDREIDEINHPGDKGRPLVTGAA"
BASE COUNT          989 a         1727 c         1710 g         1048 t
ORIGIN      
        1 ggtgatgacg gcgctcgccc agaacctgga gccgtcctgg cgaaccttta gcccctcctc
       61 tacgtaacgg ccctctgcca gcgccgcgcg cagtacgtcc tcggggtgtc cacgctccac
      121 atcttcttcc cggtagaagg acgagaagtg ccggcccagg atctcctctt ccccgtaacc
      181 ttctatacgc tcggcgcctt cgttccaggt cgttacgcat cctccggggt ctagcatgaa
      241 gatggcgtag tccttaaccc cctcgaccat cagcctgaaa cgctcctcgc tctcgcgcag
      301 actctcctga tggctccttg cctccagcgt gctcctctgg gcccgatccc tggccgagcg
      361 caacgatgaa gtcagcacac tgacaagtac cccttcgagc atgaacgcga gcacgtctat
      421 cccctcgacg ctgaatcccg tgagggtgcc cctggggttg aggaagaagt aatccgtcgc
      481 cagggcgctt atggcggtag ctagcagacc gggtcccagc ccaccgtacc aagcactgac
      541 aatgatggcg ctgaagacca gcaggaacgg cgtgtcctgt acggtcaggg ggtccagcag
      601 cagtttgatc cctagcgcgg caccgacggc cagcatcgcc acgccatagc gtagggctat
      661 ttcaagagag tgtttcatag gaacctgtca tcctgccgcg tccccgcccg cgcgggcagg
      721 ctcgcgaaac atgccatggc cggtttcccc tttgcctccc gtgtcgaggt cctgcattcg
      781 gcgacttcag cgctgtaggc tttccaggat atagtggtcc cctcttacac gcttattggt
      841 ggaccgaatt atacgttatg tgttcatcgc agcatattgc ggccggtgcg tagcatgatc
      901 ctcaagctcc ggaatccttc tcgcgacttc ccgaccacgg tggtcgccca cggtcggtga
      961 gccgggccgc cagccgtttt gcgaggctga ccaagatcag cataaccatt ccgctcgaga
     1021 gcactatgct gatgaggaca gcacctgccg taaggtacgg atgagtcgtc aagtgttcgc
     1081 cgagccaccg ggcgccccag gcgtccgccg ggtcacgtgc gaccgcttcg ctccagcgct
     1141 gcaggagcca gatggctggt gcgaacacga tggcggcacc gacgaccacg aagaggccgg
     1201 cgagtcgttc ctgtctgccc ggtaccacgc gcatggataa tccccgccct tattcgtccg
     1261 cgagaagcat ctgggctggg ttaacgttca ttgatgacct gatgcctgac acctgtagcc
     1321 cgacaactgt agcctgacac tagagtacaa tagcacccgg tcgttgtgga tcgtggcttg
     1381 aatgagctct ttccagcaac ccggttgttg aagggaacag ccggtaacgg gagtacgaca
     1441 tagccgggca aggaaatgaa gcggcgggac gacccgggct ggggggtgac tccattcctg
     1501 ggcttgttac ctccctgaaa acattctcgc ggtcttcgag catagtgacc atactcgtcg
     1561 gctgcctggt tctcgtcggt tggacgttcg cttttggaga cttcagaggc gtcctgccag
     1621 gtcctgtggc gatgaacccc gtctccgcgg tggggctctt gttggccggt gtgtcggcct
     1681 ggctgttgca ggatgagcgg gcaggtggga gggtgcgttg gatctcgcgc gtatgcgccc
     1741 tcgtggttgc cctcgttggc ctgtccaggc tgattcaggt acgtttcggc ctagagccgg
     1801 gcatcgacca gatcctgttc cccgagaggc tcgcctcggg ggctgcttcc acgggttttc
     1861 ctaacaggat ggcgtttgac acggcgctcg acttcgtcct gatcgggggc gccctgttgt
     1921 tcctggacag gcggacccgc cggggtctct gggtctcaca gtatctcgcc ctgactgtgt
     1981 tcgtgttctc cgtactggct ttcgccggct atgtttacgg cgtcgcgaat ctgtacgcca
     2041 acacctccta catccccata gcgtcgatga ctttcgtcgt gctctcggcc ggcgtcctgt
     2101 gcgcacgccc cgagcgcggt cttatggtcg tcgtcaccag ccgcaatgtg ggaggtgtgg
     2161 tggcgcgccg tctgttgccc gccgccatcc tggtcccact ggtgctcggc tggttcaggc
     2221 tcagggggca acaggtcggc ctatacggca cggaactcgg ggtatcactc ttcaccgtgg
     2281 cgaacatgtt cgtcgtcgcc gccctggttt ggtggagcgc ccgcctgctc taccgaatcg
     2341 acggcgagcg caaactggcc gaagaagaga tgcaggcggc gagggagagg gcagaggagg
     2401 ccaaccgggc caagagcgag ttcctggcga acatgagcca cgagattcgc acccccatga
     2461 acggcatcat cggtatgaca gaccttcttc tggacagccc cctggacgag gagcaacgcg
     2521 agtacgcgga gacggtgcac tactcgggcc agactctgct cgccatcctc aacgacattc
     2581 tggacttctc caagatagag gcgggcagcc taaacctgga ggccataaac ttcgagttga
     2641 ggagggaggt cgaagaggtc gccgccctgc tcgccggaca cgcccacgag aagggcctgg
     2701 aactggtcag cttcgtcgag cctggcacgc cgaccgctgt gcgtggcgac cccttccgtc
     2761 tgcggcaggt cctcaccaac atcctcggca acgcgatcaa gtttacagag gagggcgagg
     2821 tgaccctgcg ggcagatctc gtcgaggaca ctccggatac ggccacggtt tatttcgagg
     2881 ttagtgatac aggcattggc atgaccgaag aacagcgttc caggctcttc aaccccttta
     2941 cccaggccga cgcatcgacg acccgccgct acgggggcac gggactcggt ctggccatca
     3001 gcaagcaact ggtgttgatg atggggggag agataggcgt gaggagcgag cccggcgtag
     3061 gttccacgtt ctggttcacc acgcggctcg agaagagacc ggaggatagg cgggcgaccc
     3121 ctgttccccg cgcgggcctg cgcgaccttc gcgtgctcat cgtggacgac cacgagacca
     3181 atcgccgcat actgcacatg cagatcacct cctggggcat gagagacgac atggcggaga
     3241 acggggcgga ggcgttggat ctactcctgg cagcggccga aagcggggag gcttacgacc
     3301 tggcggttct ggatatgcag atgccagata tggacgggat acaactcgcc agcgccataa
     3361 gcgcagaacc cgccatctct tctaccaggc tggtgctgct tacctctatt ggcatcaaca
     3421 tcaacgagac tgcacgcatg gccggcgtgg aggtcgtcct caacaagccg gtgcggcagt
     3481 cacagctcca cgacgccctc gcgacgatgc taggcacccc tacagaggca caggccagac
     3541 cgtcgcggag aggcgtgagg cccgctccgc acgcaacacc ggcggccgaa gaacaggcgt
     3601 cccgcggtta cgtcctcctc gccgaggact accccgtgaa ccagatggtg gcgataagga
     3661 tgctcgagag gtccggctac caggtagacg ccgtgaacaa cgggagggag gccgtcgagg
     3721 cactctcgaa tatccagtac ggggcggtgc tcatggacgt tcagatgccc gagatggacg
     3781 gctacgaggc caccgccgag atccgccggc gcgagggatc cctccgtcac acccctgtaa
     3841 tcgccatgac cgccgacgcc atgcaagggg atagggagag agcactggcc gcaggcatgg
     3901 acgactacat agcgaaaccg gtaaagcatg aggagctgga ggccgtgcta gagtgttgga
     3961 tcccccaccc cgagcaggaa cccctcgcgc agaggggcga cggggctccg ggagccaacg
     4021 aagacactcc cgctctcgac ctatccgtgc tggagagccg gcgcgggccc cagcgagatg
     4081 gggaaccgga caaactggcc aggatcgtgg gtcttttcat cgacgacgtg ccgctaaggc
     4141 tggatgagtt gcggaaggct gtcgagaggg gagaggccca gaaggtcgag gagacggccc
     4201 acatgctcaa aggcggctcc ggttacatgg gagcagttca aatgtcggag atctgcgccg
     4261 ggatccaggg actcggcgcc tccggtgaac tgtggcgcgc ccccgagctg ttagacgagc
     4321 tcgaagcgga gttcaaacgc atccgccccg ccctggaagc cgcagtcacg gcgaattgag
     4381 ctagtcgacg acgtgtccac tggcgccaag agcctttcga ccctggcggt ggcgctgacg
     4441 ctcttcggtc tggccggcgc cgccgttctg gcaacggttc ctccgcaatt tatgggacca
     4501 gagcggcccg ggttcgcggt cttcctggtc gccgggttga cggtcatcgt cttgaccgcc
     4561 ggcttcttcg gctacatcta ccggcttggc atgggcttcg gcaggacggc cctggtgctg
     4621 gccgccggct acaacgccct gatagcggcg gtgaagctcg gcctggcgcc ggccgcgctt
     4681 taccaggcca atcgcgagca gtcgttcgac gcctcgatgg gagatccgaa cgacctgttg
     4741 ttctacctcg gggtcggctc gggcgtcctc ctgctctacc tgctcgtctt cggggtgatg
     4801 tacagcgtct tcaggctccg cttcagacga cgcgcactcc cgtccgaacc tccgccggaa
     4861 cagaggggcc gatggtcgaa cagaaccatt gtcgtcggcg tcgtcgtcct cgtcgctttc
     4921 gccgccagct tcctctggat catgcccgtg gtctacgtcg ggttgccgac cctctcctac
     4981 ctcttataca tcttcaccac gttcggctct gcgattacgc tcgccctgat cctggccgct
     5041 gtcctggcct acaagtcctt cgacgaggtc gagaagcgcg ccgtccgtct gggagacgcc
     5101 acccttctgg ccagtttctt ctggttgggt ctcaccctga tcgtgctcta ccacgtcatg
     5161 tgggtcatct ttctactgac gctggtcagc atctggcctt tccgaacata tacgcccaag
     5221 taagcatgaa gatgcttgcc ctctactgga gcctgctgcg ctatcgcgtg gctgtgatga
     5281 tcatcctctt catgcggctc ggggccgcgt ggcacggggg gctgacagct atcgacgcgc
     5341 cggtgatcct gacgatcctc gcccttgcgt cgagctacgt gagcgcgacg agcgtgaacg
     5401 acatctcgga cagggagatc gacgagataa accaccccgg cgacaaagga aggcccctcg
     5461 taacgggcgc ggcc
//
LOCUS       DATMJA010000015         2376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_61273, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000015 DATMJA010000000
VERSION     DATMJA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61273"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VNT12_00355"
     CDS             complement(<1..466)
                     /locus_tag="VNT12_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWI44586.1"
                     /translation="METVRTEERPRAIPAPGVTISSLIEKASSYGPEGTAELIADAYN
                     AAHAAHRGQTRKSGEPFVYHPLATADVLAELRLDPTTIAAALLHDVLEDTGVTKEELS
                     ERFGDDVAEIVDGVTKLKRLPSGNLEEAQAESLRKMIVAMSRDVRVIIIKLAD"
     gene            complement(488..2158)
                     /locus_tag="VNT12_00360"
     CDS             complement(488..2158)
                     /locus_tag="VNT12_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HWI44587.1"
                     /translation="MANGGISRADRSGNLRRFSQISRVLVRHGFGFVFDVRRDRREKK
                     GLQEFLAPNFGVRLRRTLDDLGPTFVKFGQVLSTRSDILPEGVLSELQKLQDTATPMP
                     AGLAQEIIEGELGAPVDELFASFDPVPLGSASIGQVHRAVLRGGETVAIKVQRPKAPR
                     RVAGDLELMRDFATLLDRRFGRRLFVDVRGLVAEFEVVIRRELDYSTEAENARRFAAN
                     FAGTQVVIPQVYLGLSTPRVLTEEYIEGTRFRDIRPLMLSPSGRRRVASMGAEAIFKM
                     AFEDGFFHGDPHPSNLLLTPGGDLALLDFGMVGYMSQGDIEALSRLFIAVIQRDAAAA
                     LRALERLGVSYATEVRGDLIRDLREFLNKYSGLSVGEVTLGQALSELVALARRYRLRM
                     PPVFPLLTKALVTAEGLARSIDPTINVYEVARPYAQRLLSGRYSPEAVIEVAEERALE
                     YARYVEDYPEQIRLLLTEISDGELEVHLKHGGLDELFGSVDVLANRLVFAVVTAALFV
                     GSSMLGAFYVAGPQVPFLGAPLLSFLGFTLSIIQTIILLTIIFRSGRL"
     gene            complement(2174..>2376)
                     /locus_tag="VNT12_00365"
     CDS             complement(2174..>2376)
                     /locus_tag="VNT12_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44588.1"
                     /translation="DASLQQGLRRGGLPTREDYEDLLFRIEQLEHRVRMLEDRPAAPP
                     PPAGEPEPPPERGDASETPPGL"
BASE COUNT          437 a          748 c          769 g          422 t
ORIGIN      
        1 tgtcagcgag cttgatgatg atcacacgca cgtcgcggct catagccacg atcatcttcc
       61 tcaaagactc ggcctgcgcc tcctccaggt tgcctgaagg cagccgcttg agcttggtga
      121 caccgtctac tatctcggcc acgtcgtcac cgaagcgttc cgacaattcc tccttggtta
      181 cgcccgtatc ctcgagcaca tcgtgcagca acgcggcagc gatggtcgtg gggtcgaggc
      241 gcaactcggc gaggacgtcg gccgtcgcga gcgggtgata gacgaaaggc tcgccgctct
      301 tgcgagtctg gcccctgtgc gccgcgtggg ccgcgttgta ggcgtcggcg atcaactccg
      361 ccgtcccctc gggcccgtag gaagacgcct tctcgataag ggaagatatg gttacgccag
      421 gcgccggtat ggccctcggg cgttcctctg tacgaacggt ttccacagag aaattatacc
      481 agtagggcta cagtcgtccc gacctgaaaa tgatcgtgag caggattatg gtctgaatga
      541 tggagagcgt aaacccgagg aagctgagca ggggggcgcc gagaaagggt acctgcggac
      601 ctgcgacgta gaaagcgccg agcatcgagg acccgacgaa cagggcggcc gtgacgacgg
      661 cgaagaccag ccggtttgcg agaacgtcaa cgctgccgaa cagttcgtcg aggccaccgt
      721 gtttgaggtg cacttcgagc tcaccgtcgg agatctcggt cagtagcagt ctgatctgtt
      781 cgggatagtc ttcgacgtag cgggcgtact ctagcgcacg ctcctcggcg acctcgatca
      841 cggcctctgg actgtagcgt ccggagagga gcctctgtgc gtaaggcctg gcgacctcgt
      901 agacgttgat ggttggatct atcgagcggg cgagtccttc tgcggtcacg agcgccttgg
      961 tgagcagggg gaagacgggc ggcatcctga gcctgtagcg gcgcgcgagc gcgacgagct
     1021 ctgagagcgc ctggccaagg gtgacctcgc ccacggagag cccggagtac ttgttgagga
     1081 actcccgcag gtcccgaatg aggtcaccgc gcacctcggt cgcgtagctt acgccgagcc
     1141 gttcgagggc ccgcagggcg gcggcggcgt cacgctgtat cacggctata aagagtctcg
     1201 agagcgcctc gatgtcgccc tggctcatgt agccgaccat gccgaaatct agtagggcga
     1261 ggtccccgcc aggtgtcagg agcaagttgc tcgggtgggg atccccgtgg aagaatccgt
     1321 cctcgaaggc catcttgaag atcgcctctg cccccataga ggccacgcgc cgccgcccgg
     1381 agggagagag cataagcgga cggatgtccc tgaacctggt cccctcgatg tactcttctg
     1441 tcagcaccct cggtgtcgag agcccaaggt agacctgggg gataactacc tgcgtccccg
     1501 cgaagttggc ggcgaagcgg cgcgcattct ctgcctctgt tgagtaatcc agctcgcgcc
     1561 tgataacgac ctcgaactcg gccacgaggc cgcgcacgtc cacaaagagc ctgcgcccga
     1621 acctcctgtc gagcaaagtg gcgaagtcac gcatgagctc gaggtcccct gcgaccctac
     1681 ggggtgcctt gggccgctgt actttgatgg caacggtctc cccgccacgc agcacggccc
     1741 tgtggacctg cccgatgctc gccgagccga gcggcacagg atcgaaagag gcgaacaact
     1801 catcgacggg agccccgagc tcaccctcga ttatctcctg ggcgagccct gccggcatgg
     1861 gcgtagccgt atcctggagt ttctggagct cggagagtac tccctcaggc agtatgtccg
     1921 agcgcgtgga gaggacctgc ccgaacttga cgaaggtcgg ccccagatcg tcgagggtgc
     1981 gccgcaacct gacaccgaag ttgggggcca ggaactcctg caggcctttc ttctcccttc
     2041 tgtcccgcct gacgtcgaag acgaagccaa agccatggcg caccagaacg cggctgatct
     2101 gggagaagcg cctgaggttc cccgaccggt cggcccggga gatccctcca ttcgccacgc
     2161 gcgggtcctt ctgctacagt cctggcggcg tctcggaggc gtcgccgcgc tcgggcgggg
     2221 gctccggttc gcctgccggc ggaggaggcg ctgcgggacg gtcctcgagc atcctcaccc
     2281 tgtgctccag ctgctcgatg cggaagagaa ggtcctcata gtcctccctc gtcggaagtc
     2341 cgcctctcct gagaccctgc tgcaaagagg cgtcaa
//
LOCUS       DATMJA010000016         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_831491, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000016 DATMJA010000000
VERSION     DATMJA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831491"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VNT12_00370"
     CDS             complement(<1..311)
                     /locus_tag="VNT12_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWI44589.1"
                     /translation="MIATGGRGHIILGIDPGTATMGWGVIRQEGNRLRYMQHGAITTP
                     PDWEMPRRLGRLFDGVTELVRGYRPETIAVEELFFNSNVTTAITVGQARGVALLAAYK
                     AG"
     gene            complement(502..1920)
                     /locus_tag="VNT12_00375"
     CDS             complement(502..1920)
                     /locus_tag="VNT12_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI44590.1"
                     /translation="MDVGGQARVERKGAALVLLCVAQFVDVLDVNAVIVALHSIGQDL
                     GFSQSGLQWVISAYVLFFAGFLLLSGRVADLWGRRLTFVAGLAVFTAASLCCGLSVSP
                     EMLVISRAFQGLGAATTAPAALSIITTIFAEGRERDRAVAAWTAVAAGGGAAGLLLGG
                     LITDVLGWEWIFFVNVPVGAAGIALSHVLLPESRDSAASRRLDLLGAGTVTSGLVLLV
                     LGLTRTEEAGLGSPITLATLGFAAALFGSFVFVERRADDPLVPLSLFRLHGLVGAALV
                     AFALTAATAPVGVLVTLYMQNTLRYSASLAGLAGLPFSLCVIAGSMLGGRISERIGGR
                     AAMSSGLAVVAASALVTTGITAEGGVAFVLAGAALSGLGLGCASVASTARGTSAVEKG
                     KRGLASGFMNTSAQVGTALGLASLLTLAATRTAVLSDRAEFAAEALVAGYRLAFLVAA
                     GVASLGVVSALYSFRQDKNLDT"
     gene            1978..>3101
                     /locus_tag="VNT12_00380"
     CDS             1978..>3101
                     /locus_tag="VNT12_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWI44591.1"
                     /translation="MEDVGRKKPKAGMSSGGNRVTLTRIARELGVSAMTVSNAYNHPD
                     RLSEALRERIFETAGRLGFHGPDPVGRSLRRQRTDVVGVLYSNPLSYAFDDPAAVSFL
                     GGLSTVTEEADLGLLLVPAAGGGESGERDPRAAAQAAVDGFVIYSMSDEEPLLVAALG
                     RRLPAVVVDQPRREGVPFVGVDDEGAARTAAEHLLDLGHERFAIVSFGLSPDGHEGIA
                     DSARQGRTAFHVSRMRLRGYRAALEEAGLPWFEVPVYECQGSAKELGYRAVEALFRSE
                     QQPTAILALSDQLALGAMEWMTERRISVPQDISVVGFDNIPAAASADPPLTTIHQDHA
                     EKGRVAGRMLVSQFHEEDAPSAEPLATRLVVRGSTSPIRE"
BASE COUNT          618 a         1014 c          977 g          492 t
ORIGIN      
        1 cctgccttgt aagccgcaag cagcgcgaca cccctcgcct gcccgaccgt gatcgccgta
       61 gtcacgttgg agttgaagaa gagctcctcg acggcgatgg tttcgggcct gtaaccccgc
      121 accagttcgg ttacaccgtc gaagagccgc ccgagcctgc ggggcatctc ccagtcaggt
      181 ggcgtggtga tggcgccgtg ctgcatgtag cgcaaccggt ttccctcctg ccggatcaca
      241 ccccacccca tcgtcgccgt cccagggtct ataccgagta tgatgtgtcc cctgccgccg
      301 gtcgcgatca tagtagctta aggttacccc ctaccgccac cgcttgcacc atcttctaca
      361 tcttgtgcat tggatggccg acactacaag agtcgcttgc acagaggttc gaacctctgt
      421 gcaagcattg cagctcgtca cctcttgatt actgatggct cggtgtgcgg aaggattgaa
      481 cctggctgca agcatcaggc ttcaggtatc aagattcttg tcctggcgga aggagtacag
      541 cgcggagacc acaccgagtg atgcgacgcc cgcggcgacc aggaacgcta acctgtagcc
      601 tgccaccagc gcctcggctg cgaactcagc cctatcggat aggacggcgg tgcgggtagc
      661 cgcgagggtg aggagggagg ccagccccag cgccgttccc acttgcgcgg aggtgttcat
      721 gaaacctgag gccagaccgc gcttcccttt ctccaccgcc gaggttccgc gagcggtcga
      781 agcgaccgaa gcgcagccca agcccaaccc ggagagggcg gcgccagcga gcacgaaggc
      841 cacgccaccc tcggcggtga ttcctgtggt aacgagggcg gacgccgcca ccacagcaag
      901 ccccgacgac atcgccgctc tgccgccgat tcgctcggag atccggccgc ccagcatcga
      961 accggcgatc acacagaggc tgaacggtag gcccgccagt cctgccaagg acgccgagta
     1021 acgcagagtg ttctgcatgt agagggttac cagcacaccg acaggggcgg tggcggcggt
     1081 gagcgcgaaa gcgaccagtg ccgcccccac caacccgtgc aggcggaaga ggcttaaggg
     1141 gaccagggga tcgtctgcgc gccgctccac gaacacgaac gacccgaaca acgctgcggc
     1201 gaagccaagc gtcgccaacg taattggcga gccgaggcct gcctcttcgg tcctcgtgag
     1261 ccccaggacc aatagcacca gccccgaggt gaccgttccg gccccgagga gatccagcct
     1321 gcgggaggcc gcagagtccc ggctctcggg taggagcacg tgggagagcg cgatacctgc
     1381 cgctcccaca gggacgttga cgaagaagat ccactcccag cccaaaacgt cggtgatgag
     1441 gccgcccagg agcaatcccg cagccccacc ccccgcggcc accgccgtcc aggcggccac
     1501 ggcccggtct cgctcccggc cctcggcaaa gatagtggta atgatggaga gcgccgccgg
     1561 tgctgtagtc gccgccccga gtccctggaa ggcgcgcgag atcacaagca tctccgggga
     1621 aaccgagagg ccgcaacaaa gggaagccgc cgtgaaaacg gccagacccg cgacgaaggt
     1681 caacctcctg ccccacaggt ccgccaccct ccccgacagg agcaagaagc ccgcaaagaa
     1741 caacacgtaa gccgagatca cccactgcag acccgactgc gagaacccga gatcctgccc
     1801 gatcgaatgc agggccacta ttaccgcgtt cacgtccaac acgtccacga actgcgccac
     1861 acacaacaga accagcgccg cacctttgcg ctcgacccga gcttggcctc ctacatccac
     1921 aaccctcctc cgtagataga ctttaacgat atacttttaa cgttaaagta gtgtaccatg
     1981 gaggatgtgg gccgcaagaa gccaaaggcg ggcatgagtt ctggcgggaa ccgggtgact
     2041 ctcacgagga tagccaggga gctcggagta tctgcgatga cggtctccaa cgcctacaac
     2101 cacccggatc gactctcgga ggctttgagg gagcggattt tcgagacggc cgggaggctc
     2161 ggctttcacg gtccggatcc cgtggggcgt tcgttgaggc gccagaggac ggacgtggtt
     2221 ggggtgttgt actcgaatcc gctttcttac gccttcgacg atcctgccgc ggtctctttc
     2281 cttggcggtc tgagcacggt cacggaggaa gccgatctgg gccttctgct ggtgccggct
     2341 gccgggggcg gagagtctgg agaacgcgac cctcgggcgg cggcgcaggc cgcggtggac
     2401 gggttcgtga tctactcgat gagcgacgag gaacctttgt tggtggcggc attggggagg
     2461 cgattaccgg ccgtggttgt ggatcagcca cgtagggagg gagtgccctt cgtgggtgtc
     2521 gacgacgagg gagcagcccg aacggcagcg gagcatctac tcgacctcgg gcacgagagg
     2581 ttcgccatcg tgtcgttcgg cctctcaccg gatggccacg aagggatcgc agactccgcc
     2641 cgccaggggc gtaccgcatt ccacgttagc cgaatgaggc taaggggcta ccgggctgcg
     2701 ttggaagagg ccggtctgcc ctggtttgag gtgcccgtct acgagtgtca gggcagcgcc
     2761 aaagaactcg gataccgggc cgtcgaggcg ttgttccgtt cggagcagca gccgacggcc
     2821 atcctcgcct tgagcgacca gctcgcgctg ggcgccatgg aatggatgac cgagcggcgg
     2881 atctccgtgc cccaagacat ttccgtagtc ggcttcgaca acatccccgc ggcggcgagc
     2941 gccgatcctc ccctcaccac catccaccag gaccacgctg agaagggaag agtggccgga
     3001 cggatgctgg tatcacagtt tcacgaagaa gacgccccaa gcgccgaacc actggcaacc
     3061 cgcctggtgg tccggggttc gacatctcct atccgtgagc a
//
LOCUS       DATMJA010000017         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_61442, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000017 DATMJA010000000
VERSION     DATMJA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61442"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VNT12_00385"
     CDS             complement(<1..491)
                     /locus_tag="VNT12_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWI44592.1"
                     /translation="MANDPFGARTTLNTGSEQVEIFSLKTLDERTDGDVFSLPFSIRV
                     VLESLLRNAGGIFVSKEDVEALADWPESVGGELAYLPARVVMQDFTGVPAVVDLAAMR
                     SAMENVGGDPGKINPLVPIPTEVHSSVLPCDGCGIRPGPAVYPFDPYGPFLKHLLDPG
                     DGYL"
     gene            complement(696..2309)
                     /locus_tag="VNT12_00390"
     CDS             complement(696..2309)
                     /locus_tag="VNT12_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HWI44593.1"
                     /translation="MNPSSSGRIRYLEQRGAALLGGAIAIFVGILYIGTLAPTVLPYG
                     VPDTLDSPMLQTEVSVLGVGHPSGYPTYMMLTHLFTYLPIGDPAYRVNLASAVYGAAA
                     VFAVYLAGLRLGGRPVAAAAGALAFGLSWTFWSQAVIAEVYTFEALLVALVILFLFLW
                     RDRRDSRYLLLSAFLVGLSLTHHLTSVLLVPATLAFVFLTDRRVFSRTGLMLKGVGIF
                     LLGLLPLLYLPIRALMHAPLNEADPSTPWRFLLLVTGGSFLAESSEKGRHCSPSSLTL
                     ADTSTKLGLFGEHLLEQFPLILIVVGVLAAFYLLFTDGPTAVLLGALLFGCFVQAAVY
                     LQLGIEDFYVFLIPAFLTFGLCISAGLGALLRSVESLAISSTASGVLLVILSALILAV
                     PFSGVRDSYVAHDRSGDFGGRRAIEAVAGNVEKGATVLHHRSPLWYMVLIEGRRRDLT
                     LVDPFCTSWDRQTDVVWPDQISEAEAARRYGTDDTTGVEVARRAARSGPVYLLANGRA
                     RLGSFREAGFDTVPVEKDGFLYELVPRRR"
     gene            2308..2907
                     /locus_tag="VNT12_00395"
     CDS             2308..2907
                     /locus_tag="VNT12_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44594.1"
                     /translation="MVGQNTTYTALPDGPLYTQPVAKRGYTRDTIRGGASEWDVSRPL
                     SSYARTLAMLLVHPVRFFELLPRIPDMRAPALFLLFSGLPAAILWLLFWGLYPALVAL
                     VLPLPLSFLLAGLYHLGVLSGRHGYVVTWRVLAYPLGFYLSLAAVPMLGPLGAAYSGL
                     VIMPLGLAEVHEVGRLRAVLFCAALGVVFGAAYYFSRVA"
     gene            complement(2926..>3254)
                     /locus_tag="VNT12_00400"
     CDS             complement(2926..>3254)
                     /locus_tag="VNT12_00400"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI44595.1"
                     /translation="RVRVLVAENEAGIVGGASFWIKPDLAHGDTVVEVPMLVVAEDHR
                     RAGVGKLLMEEVRNVASDSGASQIELVATRANVTAREFYRSLGFVEADVVLLEFVGSQ
                     EDPPEE"
BASE COUNT          640 a         1026 c         1024 g          564 t
ORIGIN      
        1 aggtacccgt cacctgggtc cagaaggtgc tttaggaacg gcccgtaggg gtcgaatggg
       61 tagacggcgg gtccgggacg gatcccgcat ccatcgcaag ggagaacaga tgagtgcact
      121 tcagtcggga taggaacgag cgggttgatc ttccctggat ctccgcccac attctccatc
      181 gcgctacgca tcgccgcgag gtcgacgacc gctggcacac cggtgaagtc ctgcatgaca
      241 accctggccg gcaggtacgc aagctctccg ccgaccgact cgggccagtc ggccagcgcc
      301 tcgacgtcct ctttcgagac gaatattcca ccagcgttgc gcaagagaga ctcgagcacc
      361 acccggatgg agaacggcag cgagaacacg tccccgtccg tcctctcgtc cagcgtcttc
      421 aggctgaaga tctctacttg ttcagagccg gtgtttagcg tcgtgcgcgc accgaaagga
      481 tcgttcgcca tgtctgcaac ctcttctctg acactcgtat gcgtggcctg atccgattat
      541 agatgatccg ggacgcttca ccggaaggac acccgggctc cggggcgtta ccctccctcg
      601 tacacccgca ccgaagcccc acgagccgcc tcgacaagcc ggtcatcgaa ggccaggaaa
      661 cacaggtcgg agaaccgctc cgcaagccgc acgctctacc ggcgccgggg cacgagttcg
      721 tagaggaaac cgtccttttc gaccggtacc gtgtcgaagc ccgcctcgcg gaaggagccc
      781 aatcgggcac ggccgttagc caggaggtag acgggtcctg accttgcggc cctgcgcgcg
      841 acctccacac cggtggtgtc gtctgtaccg tagcgcctcg cggcctcggc ctcgctgatc
      901 tggtcaggcc agacgacatc cgtttggcgg tcccaggatg tacagaaagg gtccaccagg
      961 gtgagatccc gccttctccc ctcgataagc accatgtacc atagcgggct cctatggtgc
     1021 aggaccgtcg cgcccttctc tacgttgccg gccacggctt ctatcgccct gcgtcctcca
     1081 aaatccccac tacggtcgtg cgccacgtag gagtcgcgca cgcccgagaa cggtacggct
     1141 agtatcaggg ccgagaggat aacgagaagg acaccgctcg cggtagagct gatggccagg
     1201 ctctccaccg atcgcagcag ggctccaagg cccgccgaga tacacagtcc gaaagtcaga
     1261 aaggcgggga tgaggaacac gtagaagtcc tcgatcccca gctggaggta aacggcagcc
     1321 tggacgaaac atccgaacaa gagcgcgccg agaagaacgg ccgttgggcc atccgtaaag
     1381 agcaggtaga aggcagccag tactccaacc actatcagga taagagggaa ctgttcaagg
     1441 agatgctctc cgaatagccc cagcttggtc gaggtgtccg caagcgtgag gctggaaggg
     1501 ctgcagtgcc tccccttctc tgaagattcg gccagaaagc tgccgcccgt gacgagtagc
     1561 aggaagcgcc acggggtcga agggtcagcc tcgttcagag gcgcatgcat cagggcccgt
     1621 atggggaggt aaagaagcgg aaggaggccg agcaggaaga tcccgacgcc cttcaacatc
     1681 agccccgtcc tcgaaaacac gcgcctgtcg gtgaggaaaa cgaaggccag agtggcggga
     1741 accagcagaa cgctcgtcag gtggtgggtg agggagagac cgaccagaaa ggccgagagc
     1801 agcaggtatc ggctgtcgcg gcggtctctc cagaggaaca agaagaggat gactagcgcg
     1861 acgaggagtg cctcgaaggt gtagacctcg gcgatcacag cctggctcca gaaggtccag
     1921 gagagcccga aagccagtgc cccggccgcc gctgcgacgg gtctcccgcc aagacgcagt
     1981 cccgcgaggt acactgcgaa caccgccgcg gcgccgtaga cggcggaggc gaggttcacc
     2041 ctataggcgg ggtcgcctat cggcaggtaa gtaaagaggt gtgtcagcat catgtaggtt
     2101 gggtagccgc tcggatggcc gacaccgagc acagagacct cggtctggag catcggggag
     2161 tcgagcgtgt caggaacacc gtaaggcaga acagtgggcg ccagggtccc gatgtagagg
     2221 ataccgacga agatggcgat ggcccccccg agcaaagctg cgcccctctg ctcgagatat
     2281 ctgatcctgc cgctggagga gggattcatg gtagggcaga atactacgta caccgcccta
     2341 cccgatggtc ctctgtatac tcagcccgtg gcaaagcgtg gctacacgcg ggacacgatc
     2401 cgaggcggcg ccagcgagtg ggatgtctcg cggcctcttt cgagctacgc gcgaacgttg
     2461 gcgatgctac tcgtgcaccc ggtcaggttc ttcgagctct tgccaaggat tccggacatg
     2521 agggcgcctg ccctctttct cctcttctcc ggcctgcccg ccgcgatctt gtggctgctc
     2581 ttctgggggc tctatcctgc gctggtagcg ctcgtactgc cactgccgct atccttcctc
     2641 ctcgccggcc tctaccatct cggcgtcctc agtggccggc acgggtacgt cgtgacctgg
     2701 agggtcctgg cgtacccgct cggtttctac ctgtccctgg ccgcagtccc tatgctcggg
     2761 cctctgggag ccgcttattc agggctcgtg atcatgccgc tcgggctcgc ggaggtacat
     2821 gaggtcggaa ggctgagggc cgtgctcttc tgcgctgccc tcggtgtcgt gtttggggca
     2881 gcttactact tctcgcgcgt cgcctgaagc agaccctgac gaaggttatt cttcgggcgg
     2941 gtcttcctga gaccctacga actcgagcaa aacaacgtcg gcctcgacga agccaaggga
     3001 gcggtagaat tcgcgggccg ttacgttcgc gcgggtggcg acaagttcga tctgggatgc
     3061 tccgctgtcg gaagcgacgt tgcgcacctc ctccatcagg agcttgccta ccccggcccg
     3121 cctgtgatcc tctgcgacga ctagcatcgg cacttcgacg acggtgtcac cgtgggcgag
     3181 gtcgggtttg atccagaagc tcgcccctcc cacgatccca gcctcgttct cggcgacgag
     3241 gacccgcacc ctgg
//
LOCUS       DATMJA010000018         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_61716, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000018 DATMJA010000000
VERSION     DATMJA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61716"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VNT12_00405"
     CDS             <1..780
                     /locus_tag="VNT12_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44596.1"
                     /translation="DIDEDGEDEVILKGEHLYAMIAPEHGGRLIYLFTLTPEGGALVV
                     GNPTDDWNFQEELNRYMDHPSNHPGALADVGYEHDRYLVSALSDGTHAWVEMTNVEED
                     SHLFGTRKSVLLAPGHPALAVCYRTPEGSGDLATETCLAPDYYRLLREGRRGLSPCNG
                     EGWRGFRNGDVAVWLGLAGDEATGWTEPAQAEAGHGMNVRVLADASHFHLLIACGDTD
                     EDSCQQLLQKHRAALDEIGEIASTVRLRDGVLLCGNGESKC"
     gene            774..2582
                     /locus_tag="VNT12_00410"
     CDS             774..2582
                     /locus_tag="VNT12_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI44597.1"
                     /translation="MLRGIFGRFTSSKGQGERSALVALRTFLPFMRPHWRGFVPAVIG
                     VIAASLVALLKPWPLGFLINHVLQVGQSDTARLASPTIMIIAVAGAIVGIAALQGLFN
                     FLKEFFLSATSERVAFSVRRALFAHMQRLSLTFHDRQRTGDMITRVTSDVTKVQELVT
                     DKLLVDGISSMLQFIGMLTVMFVIDWRIGLIATAWAPLVVIASSYFRRRIREEEQDVR
                     EREGDFTSLTQETISSIRVVKAFARERFAEERFAEQTGEMAEGNVNVARLEARFSWIM
                     TILTAGGLATVVAFGAHQVLAGALSVGTLVVFTQYMKDLQSPLNTLSRLWAKLPRVAV
                     RAERIMEVFDEHPAVEELPGAQTAPRFRGEVRFEGVSFGYDPDKPVLRDIELEIQPGE
                     VAAVVGATGAGKSTLSSLILRLYDPARGSVLIDGRDIRDYKPDSLVEQIGVVLQESLL
                     FQTTIRENIAYGSSKATFEEIQEAARVAYCEDFIRKLPNGFDTVVGERGGTLSGGQRQ
                     RIAIARAVIRDSPILVLDEPTTGLDAESEEIVMRALERLMEGRTTLMIAHKLSTVSRA
                     DKIYVLEEGTIAEEGSHEELMAKRGSYARAYQLQTI"
BASE COUNT          505 a          821 c          898 g          453 t
ORIGIN      
        1 gacatagacg aagacggcga ggacgaggtg atcctgaagg gcgagcacct ctacgccatg
       61 atagcgcccg agcacggcgg gaggctcatc tacctcttca ccctaacgcc ggaaggcggc
      121 gccctagtcg tcggcaaccc caccgacgac tggaacttcc aggaggagct caaccggtac
      181 atggatcatc cgtccaacca tccgggcgcc ctggccgatg tgggttatga gcacgaccgc
      241 tacctggttt ccgccctcag cgacggcact cacgcctggg tggagatgac caacgtcgag
      301 gaggacagcc acctgttcgg gacccgcaag agcgtgctcc tggcgcccgg ccacccggcc
      361 ctcgcggtct gctaccggac tcccgagggg tccggcgacc tagccacgga gacgtgcctc
      421 gcgccggact actaccgcct gctacgggaa ggtcgccgcg gcctgagccc gtgcaacggc
      481 gagggctggc gcggttttcg caacggagat gtcgccgtct ggttggggct ggccggtgac
      541 gaagcgactg gctggacgga accggcccag gcggaggcgg gacacgggat gaacgtccgc
      601 gtgctggccg acgcatctca tttccacctc ctgatcgcct gcggcgacac agacgaagac
      661 tcgtgccagc aactcctcca gaagcacagg gctgcgctcg acgagatagg cgagatagca
      721 agcactgtgc gtctcagaga cggtgtcctg ttgtgtggta acggggagag taagtgctga
      781 gaggtatatt cggacggttc acctcttcga agggtcaggg cgagcggtct gcgctcgtgg
      841 ccctacggac ctttctgcct ttcatgcggc ctcactggcg agggttcgtc ccggcggtga
      901 tcggggtcat cgcggccagc ctggtcgccc tgctaaagcc ctggccgctg ggattcttga
      961 tcaaccacgt tctgcaggtc gggcagtcgg ataccgccag gctggcttcg cccaccatta
     1021 tgatcatcgc cgtcgccggc gcgatagtgg gcatcgccgc gctacagggg ctgttcaact
     1081 ttctcaagga gttcttcctc tcggctacga gcgagcgggt ggcgttcagc gtacgtcgcg
     1141 ctctcttcgc gcacatgcag cgcctatcgc tcacctttca cgacaggcag cgcaccgggg
     1201 acatgatcac gcgcgtcacc agcgacgtca cgaaggtgca ggagctggtt acagacaagc
     1261 tactcgtgga tgggataagc agcatgctgc agttcatcgg catgctcacc gtcatgttcg
     1321 tcatcgactg gagaatcggc ctgatcgcca cggcgtgggc accgctcgtc gtgatcgcct
     1381 cctcgtactt ccggcggcgc atcagggagg aggagcagga cgtgcgggag cgagagggag
     1441 actttacgtc cctgacacag gagacgatct cctcgatacg ggtcgtcaaa gcgttcgccc
     1501 gggaacgctt cgcggaagag agattcgcgg agcagactgg cgagatggcg gagggcaacg
     1561 tgaacgtggc gcgcctggag gctcgtttct cctggattat gaccatcctg acggcgggcg
     1621 ggctggccac ggtcgtcgcc ttcggcgcgc accaggtcct cgcaggggcg ctctccgtag
     1681 gcacgctcgt cgtcttcacc cagtacatga aagacctcca gagcccgctg aacacgcttt
     1741 cgaggctctg ggccaagctc ccgagagtcg cggtgcgggc agagcgcatc atggaggtgt
     1801 tcgacgagca cccggcggtg gaagagctcc ctggcgcgca gaccgcgccc cgcttccgag
     1861 gggaggtacg cttcgagggc gtctccttcg gttacgaccc ggataagccg gtgctgcggg
     1921 acatcgagct ggagatccag ccgggcgagg tggctgcggt cgtgggggcc accggcgcgg
     1981 ggaagagcac tctgtcgagc ctgatcctgc gcctctacga tcccgcgagg ggctccgtgc
     2041 tcatagacgg gcgcgacatc cgcgactaca agccagactc gctggtcgag cagatcgggg
     2101 tggtcctcca ggagtcgctg ctcttccaga cgaccatccg ggagaacatc gcgtacggca
     2161 gttccaaagc gaccttcgag gagatccagg aggccgcccg tgtcgcttac tgcgaggact
     2221 tcatccggaa gctgccgaac gggttcgaca cggtggtggg ggaacgaggc gggacgctat
     2281 cgggaggaca aaggcagcgc atcgccatcg cccgcgccgt gatccgggat tccccgatac
     2341 tcgttctgga tgagccgacg accgggctcg acgccgaatc ggaggagatc gtgatgcgcg
     2401 ctttggaacg gcttatggag ggtcgcacga cgctgatgat cgcccacaag ctgtcaaccg
     2461 tgagccgggc cgacaagata tacgtcctcg aggaaggcac gatcgccgag gaggggtcgc
     2521 acgaggaact catggcgaag agaggaagct atgcgcgcgc ctatcaactg caaacgatat
     2581 agccgacgat ttgtcgtggc gcgatcctgc aactcgagag gaaggtggcc tcatgtctaa
     2641 gtccgcagag aacgctctat gagagccaag caacaag
//
LOCUS       DATMJA010000019         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_2797, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000019 DATMJA010000000
VERSION     DATMJA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2797"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VNT12_00415"
     CDS             <1..699
                     /locus_tag="VNT12_00415"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWI44598.1"
                     /translation="DVLVVQGTVVSAEHVSRSVEPLNLMEFVPSLNVPVVVGGCASYS
                     TALHLMRTGAVGVLVGVGPGRICTTRGVIGVGVPQATAVADAAAARMRHYLETGEYVN
                     VIADGGMRTGGEIAKAITCGADAVMLGSAFARAEEAPGRGYSWGMATFHPTLPRGTRI
                     STRTTGSLEEILVGPARENDGTLNLMGALRTSMATTGYQNIKEFQKAEVMYSPSLATE
                     GKTEQRSQGVGMGR"
     gene            779..2311
                     /gene="guaA"
                     /locus_tag="VNT12_00420"
     CDS             779..2311
                     /gene="guaA"
                     /locus_tag="VNT12_00420"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWI44599.1"
                     /translation="MEDPLILVLDFGGQYAQLIARRVRECRVYSEMIPYDTPLGEIRT
                     KNPEGIILSGGPNSVYEEDAPRVEPDLFDLGVPMLGICYGMQLMALVEGGEVGAVQIR
                     EYGRSDLVVKSHEGLFAGTPDEQKAWTSHGDAVFSVPEGFEITAETPSVPIIAFENPK
                     QGRFGVQFHPEVRHTEYGMEILKNFLYEACGCRPEWTPVNIITDAVERIREQVGDARA
                     ICGLSGGVDSATAAMLVHRAIGDNLTCVFVDHGLLRHNEAEQVVEAFGQNSDVPLVHV
                     DAAGRFLDKLADVTDPEAKRKIIGEEFIRTFEEEADKLEDATFLVQGTLYSDVIESGT
                     RDAARIKSHHNVGGLPEVMDLDLVEPLRNLFKDEVRVVAAELGMPERLVWRQPFPGPG
                     LAIRVIGDVTAERLEILRKADAVLQDEIRNAGLYRELWQSFAVLPAIHSVGVMGDART
                     YAYPIVIRAVTSDDAMTADWARLPYDLLERTSNRIINEVAGVNRVALDITSKPPGTIE
                     WE"
BASE COUNT          514 a          764 c          818 g          415 t
ORIGIN      
        1 gacgtactcg tggtccaggg gaccgtggtc tccgccgagc acgtctccag gtccgtcgaa
       61 ccgttgaacc tgatggagtt cgtgccttcc ctgaacgttc ctgttgtagt cggcggctgc
      121 gcgagctact cgacggcgct ccacctcatg cggactggcg ccgtcggagt cctcgtcggt
      181 gtcggaccag gacgcatctg cacaacgcgg ggtgtgatcg gggtcggggt gccgcaggct
      241 acggccgtgg ccgacgccgc agccgcccgt atgcgccact acctggagac cggtgagtac
      301 gtgaacgtca tcgccgatgg cgggatgcgc actggcggtg agatcgccaa ggccataacc
      361 tgcggggccg acgccgtaat gctgggcagc gccttcgcca gggcggagga ggcccccggc
      421 cgcggctact cgtgggggat ggccaccttc cacccgaccc tccctcgcgg cacacgcatc
      481 agcaccagga ccacggggtc gctggaggaa atactcgtcg ggccggcgcg ggagaacgac
      541 gggacgctca acctcatggg cgccctgagg acgagcatgg cgacgacggg ctaccagaac
      601 ataaaggagt tccagaaggc cgaggtcatg tactcgccct cgctcgcgac agaaggcaag
      661 accgagcagc gctctcaggg cgtcggaatg gggagatgac cgcgcagcgg tcatcgggga
      721 gtagggagat aggggaaact actctctact ccccactccc tgctcccaaa atccacctgt
      781 ggaagatccc ttgatcctgg tactcgactt cggcgggcag tatgcgcaac tgatcgcgcg
      841 ccgcgtcagg gaatgccgcg tctactcgga gatgattcca tacgacacgc ccttaggaga
      901 gataaggacg aaaaaccccg agggcatcat cctctcgggc ggacccaaca gcgtctacga
      961 ggaggacgct ccgcgcgtgg agccggatct cttcgacctc ggcgtgccga tgctcggcat
     1021 ctgttacggg atgcagctca tggcgctcgt tgagggtggg gaggtggggg ccgtccagat
     1081 ccgggagtac ggtcgctccg acctggtcgt caagagccac gagggcctct tcgccggcac
     1141 gcccgacgag caaaaggcct ggacgagtca tggagacgct gtcttctccg tcccggaagg
     1201 cttcgagatt actgccgaga caccctcggt tccaattatc gcgttcgaga accccaaaca
     1261 ggggcgtttc ggggtccagt tccatcccga ggtaaggcac acagagtacg ggatggagat
     1321 cctcaagaac ttcctctacg aggcctgcgg ctgcaggccc gagtggacgc cggtcaacat
     1381 cataaccgac gcggtcgaga ggataagaga acaggtaggc gacgcaaggg ccatctgcgg
     1441 actctccggc ggggtcgatt ctgcgacggc ggcgatgctc gtccacaggg ccatcggcga
     1501 caatctgacg tgcgtctttg tcgaccacgg tctcctgagg cacaatgagg ctgagcaggt
     1561 cgtcgaggcg ttcggccaga actccgacgt gccgctcgtc cacgtagacg cggcgggacg
     1621 tttcctggac aagctcgctg acgtcacgga ccccgaggcg aagcgcaaga tcatcggtga
     1681 ggagttcatc cgcaccttcg aggaggaggc ggacaaactc gaggacgcca ctttccttgt
     1741 gcagggcacg ctctactccg acgtcattga gagcgggacg cgcgacgccg ccaggataaa
     1801 gagtcaccac aacgtgggcg gcctccccga ggttatggac ctcgacctcg tcgagccttt
     1861 gaggaacctc ttcaaggacg aggtacgcgt cgttgcggcg gagctcggga tgcccgaaag
     1921 gctcgtctgg aggcaaccgt tccccgggcc agggctcgcg atcagagtca taggggacgt
     1981 cacggcagag cggctcgaga ttcttcgcaa ggccgacgcc gtgttgcagg acgagataag
     2041 aaacgcaggg ctctaccgcg agctgtggca gagctttgca gtattgccgg ctatacactc
     2101 cgtcggcgta atgggcgatg cgcgtaccta cgcctacccg atagtgattc gggccgtcac
     2161 ctcagacgac gcgatgaccg cagactgggc ccgcctgcca tacgatctgc tcgaacgcac
     2221 gagcaaccgt atcatcaacg aggtcgccgg cgtcaaccgc gtcgccctcg acattacgag
     2281 caaaccacca ggcaccatag agtgggaatg aagtagcact tcagcctgtc agcgtcagct
     2341 agatcgcgga tggcggaggg agggttctct cgagaggcgg ttcacgaaag caaacgattc
     2401 gccttcgagc acatgcagcg accgacacta aaaagctgac tggcagaatt gctgacgagc
     2461 gcccgacagg acgcgtgctg aaaggctatt taacctagat ttaacgagga g
//
LOCUS       DATMJA010000020         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_180893, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000020 DATMJA010000000
VERSION     DATMJA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180893"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2224)
                     /locus_tag="VNT12_00425"
     CDS             complement(<1..>2224)
                     /locus_tag="VNT12_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWI44600.1"
                     /translation="YFEEYVKHYTNAATLVTEEFEDMEVTGLFSGWNEEKQEYDPSSW
                     RYEGQPIDYAAVGGSQGRVVGEEVGGEPGMHEGRPRPNDPTLQHPRCVFQILKRHFSR
                     YTPEMVERVTGCPKEAFFKVAEAICENSGPERTTALCYAVGWTQHSKGVQIIRTAAIL
                     QLLLGNIGRPGGGIMALRGHATIQGSTDIATLYNILPGYLPMPDANKDHETWKDYVED
                     ETAKTGWWANFPKYITSQMKAWYGEKFDPEGGELFDQFPRLNGDHSYYPTIMSMMDRD
                     VKGAFVFGQNFAVGGPHARLARDGARNLDWLVVLDAYEVETADIWKMDGVRPEDCGVE
                     VFFVPAALVAEKEGSFTQTQRMLQWHDKSVEPPGDARSDAWFLFHLGRRIKDLYKRSR
                     KKRDALIRAMTWDYPTEGERDEPDIESILKEVSGYKVETGEPVSGFAELKDDGSTACG
                     GWIYSGVYADGVNQARRRKPRGEQDQVAAEWGWAWPANRRILYNRASADPEGKPWSER
                     KKYIWWDEEQEKWVGHDVPDFQADKAPSYRPDKEASGVDAIPGDGPFIVNGDGLGWLL
                     APVGLKDGPLPVHYEPVESPVPNLLYNRIRHTPAATFLDHPDYPDNPPEDPRFPYVVT
                     TYRLTEHHTSGAMSRWIPWLSELQPELFAEISPELAEEKGIGNTDWVTISTNRGKIHC
                     KVLVSDRIPVYDFEGRRIHTIGLPYHWGPKGVVKGDIVNDLMPPIALEANVAIHEAKA
                     "
BASE COUNT          340 a          725 c          694 g          465 t
ORIGIN      
        1 aaggccttgg cctcgtggat ggcgacgttg gcctccagcg cgatcggcgg catcaggtcg
       61 ttgacgatgt cacccttgac cacccccttc gggccccagt ggtaggggag gccgatggtg
      121 tgaatcctcc gcccctcgaa gtcgtagacc gggatgcggt cggagacgag caccttgcag
      181 tggatcttgc cgcggttcgt cgagatagtc acccagtcgg tgttgcctat ccccttctct
      241 tcggccagct cggggctgat ctcggcgaag agttcgggct gcagttcgga gagccaggga
      301 atccaacgac tcattgcccc gctcgtgtga tgctcggtga gccggtaggt cgtgactacg
      361 tacggaaagc gcggatcttc cggcgggttg tcggggtagt ccggatggtc gagaaacgtg
      421 gctgccgggg tgtgtcggat tctgttgtag agcaggttcg ggaccggtga ctcgaccggc
      481 tcgtagtgca ccggcagcgg gccgtccttc aagcccacgg gggccaacag ccagccgagc
      541 ccgtcgccgt tcacgataaa cgggccgtcg ccggggatgg cgtcgacccc gctggcttcc
      601 ttgtccgggc ggtacgaagg cgccttgtcc gcctggaagt ccggcacgtc gtggccgacc
      661 cacttctcct gctcctcgtc ccaccagatg tactttttcc tctctgacca gggcttgccc
      721 tcaggatcgg cagaggcgcg gttgtagagg atgcgccggt ttgcgggcca ggcccagccc
      781 cactccgcgg ccacctggtc ctgctcgccc cggggttttc tccgccgggc ctggtttacg
      841 ccgtcggcgt agacgcctga gtagatccaa cccccacacg cggtggagcc gtcgtccttt
      901 aactccgcga acccgcttac aggctcgccg gtctcgacct tgtaaccgct tacctccttg
      961 agaatgctct ctatgtcggg ctcgtcgcgc tcaccctcgg tcgggtagtc ccaggtcatc
     1021 gccctgatga gggcgtcgcg cttcttcctg ctcctcttgt agaggtcctt gattctgcgt
     1081 cccaggtgga ataggaacca ggcatcactc ctcgcgtctc ctggcggctc cacggacttg
     1141 tcgtgccact gcaacatgcg ctgggtctgg gtgaaggagc cctccttctc ggccaccagc
     1201 gccgcaggaa cgaaaaagac ctcgacaccg caatcctcgg gcctgacccc atccatcttc
     1261 cagatatcgg cggtctcgac ctcgtaggca tcgagcacca cgagccagtc gaggttcctg
     1321 gccccgtcgc gcgccaggcg ggcatgcggg cctccaacgg cgaagttctg cccgaagaca
     1381 aaggcaccct tcacgtcccg atccatcatg ctcatgatcg tggggtagta cgagtgatct
     1441 ccgttgaggc gcgggaactg gtcgaagagc tctccgccct cgggatcgaa cttctcaccg
     1501 taccacgctt tcatttggct ggttatgtac ttggggaagt tggcccacca gccagtcttg
     1561 gcggtctcgt cctctacgta atctttccac gtctcgtggt ccttgttcgc gtccggcatc
     1621 ggcaggtagc cggggaggat gttgtagagg gtcgcaatgt ccgtcgagcc ctggatcgtg
     1681 gcgtgcccgc gcagagccat gatcccgcct cccggacgcc ctatgtttcc gaggaggagc
     1741 tgcaagatgg cagcggtgcg gatgatctga acccccttgg agtgctgggt ccatcctacc
     1801 gcgtagcaca gcgccgtagt cctctcgggg cccgagttct cgcagatggc ctcggcgacc
     1861 ttaaagaaag cttccttcgg gcagccggtg acacgctcga ccatctccgg ggtgtacctg
     1921 gagaagtggc gcttgaggat ctggaagacg cagcggggat gctggagcgt ggggtcgttc
     1981 gggcgtggcc gaccctcgtg catcccgggc tctcctccca cttcctcgcc cacgacgcgg
     2041 ccctgactcc ccccgacggc ggcgtagtct atgggctgac cttcgtagcg ccagctcgac
     2101 gggtcgtact cctgcttctc ctcgttccag ccggagaaga ggccggtgac ctccatgtcc
     2161 tcgaactcct ccgtaaccag cgtggcggcg ttggtgtagt gctttacgta ctcctcgaag
     2221 tact
//
LOCUS       DATMJA010000021        16808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_832384, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000021 DATMJA010000000
VERSION     DATMJA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832384"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VNT12_00430"
     CDS             complement(<1..193)
                     /locus_tag="VNT12_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWI44601.1"
                     /translation="MTPKLGCVGLGNIGEPVVRHLLLAGYEVLVHDTSAEAVSRLDDT
                     PARPVGDLKALASAADAVLL"
     gene            complement(407..1588)
                     /locus_tag="VNT12_00435"
     CDS             complement(407..1588)
                     /locus_tag="VNT12_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI44602.1"
                     /translation="MVVDDGLPKATDYFSLDELLTEEERDVRDKVRSFCDADVVPVIN
                     SYWQNEAFPFELLPKMAALNVAGTTIEGYGCPGMSPTAAGLVSRELARGDGGLTTFFA
                     VHSGVAMTSISMLGSEEQKDKWLPPMARMEKIGAFGLTEPNHGSDAALLETAARREGD
                     EYVIDGSKRWIGNASFADVTVIWAREEHGKVGAFLVERGTPGFEVTITTGKTSQRTVL
                     NTDVRLEGVRVPAENKLANSKGFGDAVMVLTPTRHNVAWEAVGHAISAYESALAYARE
                     RVQFGRPIASFQLVQSKLANMLADITAMQTLCFRLSQLRAQGRMTIGAASLAKMHNAQ
                     RAYQVVADARDILGGNGILLEYHVARHLADMQAVYTYDGTDTVLSLVVGREITGQQAF
                     S"
     gene            complement(1866..3227)
                     /locus_tag="VNT12_00440"
     CDS             complement(1866..3227)
                     /locus_tag="VNT12_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI44603.1"
                     /translation="MGFFVDMFDIYLPIVVLAPAIIYFVSPQMNEATTAIVNGSIFAA
                     TLLGRPIGAFIFGHLGDTIGRRRTTIIAVSGFGVATLLIAFLPGYAQWGIAAAIIFIL
                     LRFIDGIFLGGEYTAASPLAMEYSPKEKRGFFGALIMTGYPLAYATISLITLILVQLI
                     PAGGLNSPYVQWGWRIPFVIGALMAFALVYYYVTSVAESEVFEAAAESESEGGGRSPI
                     MELFKGPNLSSFLQVFVMMSGFWLTLYTAAAMLPGLLTSELKLSPTNLTITLVIAYVI
                     LAAAYIAAGVIGQRIGRRLFLIAASVIMATVGTFLYYVLVGTAPQNLFAVIVLVTIVT
                     VLITWVWALATVYINERFHTGVRASGFGIGYSLAVIPPAFYAFYQSGLASFMPFKYTP
                     LPLLVIGALLILIGAVWGPETKDVDFAEEPEVEAAREHAASEVSANGGSRTARDADAR
                     SRS"
     gene            complement(3484..4710)
                     /locus_tag="VNT12_00445"
     CDS             complement(3484..4710)
                     /locus_tag="VNT12_00445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI44604.1"
                     /translation="MSAATSKGPLSGVRVVDISTSYAGPTATMYLGDMGADVVKVERP
                     ETGDDARYWGPPFVGGEAAWFLSANRNKRSVCLDISREEGFAALMRLLDGADVFVENL
                     NPGKLKRRDLDPEAICARFPRLIYCALSGFGLDGPDRDRPGYDLIAQARSGLMSVTGE
                     FGGTPQRVSTALSDIVAGMAAAFAVASTLVRRQRTGEGELVDVSLLEADLALMAPRIA
                     SYLAGEPEPRPSGGTDSVLAVYQPLETADRPIVVAVGNDKMWVRFCEALGLEDLAEDP
                     GLATNAGRRERRGEVIEKVQERLLKRPAKYWLERLGEAAVPCQAIQFLSEVIEDPQVE
                     ARSPIVEVEHPTAGTVRTVGSPWQLGSNGKHHRPAPMLGADTRAVLGEAGYTEKEIDA
                     LVEKGVAWTGNATVRP"
     gene            complement(4707..5684)
                     /locus_tag="VNT12_00450"
     CDS             complement(4707..5684)
                     /locus_tag="VNT12_00450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HWI44605.1"
                     /translation="MLVKQFGALEQMSLEDLPTPVPGQGEVLIDARAIGVNFPDLLVI
                     EGTYQILPELPFSPGKEVAGVVRAVGPGVASPNVGERVTAQVEHGAYREQVLAEANNV
                     AVLPESVGYEAGAAFGLGYLTVYFGLLRRARLEPGEWVLVTGASGGVGSAGVQLAKAL
                     GARVIAVGRTEDKRNFALEQGADHTIEFKPEGFKERVMELTDGHGADVILESVGGELF
                     RACMRAIAWEGRLVIIGFAAGEIPTVKAGHVLVKNMDLIGVQSSDYREREPESFREAQ
                     EELLGLLTHGKIGVQVTATYPLEAAAEALEEIRDSRVLGKVVLTTGASA"
     gene            complement(5696..6823)
                     /locus_tag="VNT12_00455"
     CDS             complement(5696..6823)
                     /locus_tag="VNT12_00455"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI44606.1"
                     /translation="MAITPLEGTRVLDFTTLLPGPLATLILAEAGARVIKIERHGRGD
                     EMRSYEPKFGTDSVNFALLNRGKESLEVDLKSPEAWALLRPLIERADVLVEQFRPGVM
                     KRLGLDYNAVAEINPRLVYCSISGYGKEGPRAGVAAHDLNYVSETGLLGLTAGPDGAP
                     VLPQALIADIGGGAYPAVMNILLALLERERTGEGAYLDVSMSENVFTFLYWAIGNGTA
                     VGEWPEPGNDLLTGGSPRYNIYRTADGHYVAAAPLEEKFWRRFCDILGLEETSRDDSR
                     DPGATKTAIAERIASLPADHWRERFEGEDVCCTVVRGVREALEDEHFKARGVFDRSVE
                     DGDGREIPALPLPVVPGFRRPELRLGYPALGEDNASLEEGG"
     gene            complement(7069..7818)
                     /locus_tag="VNT12_00460"
     CDS             complement(7069..7818)
                     /locus_tag="VNT12_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWI44607.1"
                     /translation="MTFKPQPVARAREQVETQIREAILSGTFKRGEKLPSEAELAESF
                     AVSRTTIREALRSLAAAGLIFKVPGASGGSFVQVIDYRSLSGVLGDSIVNTLRLGTVE
                     YDEVAQVRYLLEVPSAKLAAANRTEEDVETLRTIVDRQKEITFDHPDVPRLDIDFHSA
                     IGDASGNRVLASFVTALHQAIRPVLAKHLDAKAGRDTVRQHAAIVKAISESDAEATAA
                     AMEKHLNYLQKLRDVRDEPPTSNGSRKRGSS"
     gene            complement(8002..9699)
                     /locus_tag="VNT12_00465"
     CDS             complement(8002..9699)
                     /locus_tag="VNT12_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI44608.1"
                     /translation="MFTSEELVAIPESRLTPELVTRYGKEGAWREVTLDSYLAAATAA
                     GPDKLAAVSVDGQTGERTASASYGELNELVDRLVGGLSREGVGPGDTVSLMLPNRLEF
                     GALIFAISRLGAVYSGIPVSYGEKDAALMLWRARAKVLVIPSGESRTDHVAMAGRLLS
                     ETPELGHVVVLGDEAPEEPGWLSFEELASAEPLDLPPVVAPGSLAHVGFTSGTTGEPK
                     GVMNTHQMLDFVCRRWVEHQGQEMLNRDPVNLIPSPVGHHTGFLWGVLMTALLGGTAV
                     YLDRWEPKVVAQVIKREGVTAMIGAPTFLQDLVRHPGIDPETCPSLRIISIPGAPIPR
                     SLVPQAREMLGCFICPAWGMTEWGIGISGSPRLPLERVEKTDGVPVEGCEVRVVQDTG
                     EPAPPGQMGDLQIRGPGLFAGYLARPDFTAEAFTEDGWFQTGDLAIMEEDGYVSLVGR
                     SKDIVIRGGENIPVHRLENLLYEHPAIADVAVVGVSDERLGERACAVVVLEPDQHLAL
                     EDVTEYLLGEGLSKHFLPERLELMEKLPKTQSGKIRKFEIRNRLAPSAEQRPDEHAR"
     gene            complement(9936..10712)
                     /locus_tag="VNT12_00470"
     CDS             complement(9936..10712)
                     /locus_tag="VNT12_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWI44609.1"
                     /translation="MSFEDLRVDHDGGIATITINRPDKGNMFRRKTTLELAEALRSLR
                     DDPELRVGILTGAGDRFFCIGGEHEPFEGYDHSVVLPIVDVYELLDTIPKPVVAAVNG
                     FAVGGGNVLHTMCDLSVASENAVFRQVGPMVGSYDAGFGTWYLEEAIGRKRAKEMWYL
                     NHKYSAEQALAIGLVNEVVPQERLMERVWELSGEMISRGPHALAALKTAFAGRHLGVL
                     GQARMAHDLLLTQYLRTEEAKELSAAFSERRPPDSDRFNR"
     gene            complement(10709..11584)
                     /locus_tag="VNT12_00475"
     CDS             complement(10709..11584)
                     /locus_tag="VNT12_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWI44610.1"
                     /translation="MSDNGNVAVLGFGTMGAGIAQVCALAGSEVVVLEESEERLEDGR
                     QRLEAFLGEGVRREKVMEEERNEALARVRGTTEVGDLAGAGVVIEAVVEDLETKRALL
                     PVVADVVGEGAIMATNTSALSVTELAATVPRPERFGGLHFFNPAPLMRLVEVVAAVQT
                     SEETLDFLESFAKEIGKEPVRTKDRPGFLVNRLLMPYLNQVVQAYDDGLATAEDMDVA
                     LEGGLGYPMGGLKLLDLIGLDVHLYATSAAYEQTHDPHLAPPPLLSRMVDAGYLGRKS
                     GRGFHDYSDGNGGRS"
     gene            complement(11622..12521)
                     /locus_tag="VNT12_00480"
     CDS             complement(11622..12521)
                     /locus_tag="VNT12_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HWI44611.1"
                     /translation="METGSREPQPAESARDKDRLRVGILGAGTMGSGIAITVARAGHE
                     VVVRDISQERVEDGLARVKQFLDRSVERGRLDAERRDEALASTSGTTEIEALAGCDVV
                     IEAIFEDEALKAETFGQLDEICGPEALFHTNTSTLSVTGIAAGSTHPERVVGTHYANP
                     APLVDLVEVTRGRLTSDEALRRSVAFVEGLGKRVVFTKDSPGFILNYFLVPFENDCIR
                     ALEAGMGTVESIDKAVTLGLGYPMGTFKLLDIVGLDIHKAVSMSLYQQLRDPRFAPPP
                     LVDRMISANHLGRKTGKGFYEYE"
     gene            complement(12712..13020)
                     /locus_tag="VNT12_00485"
     CDS             complement(12712..13020)
                     /locus_tag="VNT12_00485"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HWI44612.1"
                     /translation="MTALWITIVVVALASAAIRALGPILVGGRELPPSMGAVIALLVP
                     ALLTALVLTQTFGEDGRLVLDEKAMGVAVAAVALSLRAPVLVAVTLAVATTALARAFG
                     "
     gene            complement(13017..13700)
                     /locus_tag="VNT12_00490"
     CDS             complement(13017..13700)
                     /locus_tag="VNT12_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWI44613.1"
                     /translation="MSSEGECGGGVYLAGARAVLPLAMATGVMGISFGVLARSLGWGV
                     VAPMVFSVIAFSGTAQLAVVSVLGGGGGVVPAIVAAVLLNARFLPMGIAAAPFLKGGP
                     LRRALEGQAVVDASWAMASRGGGRFDREFMIGATIPQVALWIVGTVVGVLVGGVVGNV
                     ERLGLDVIFPAFYLTLLVEELRAGGQTIAVVLIAAALSLALVPFTPPGIPVIASCAAA
                     LLGLRGRSS"
     gene            complement(14286..15275)
                     /locus_tag="VNT12_00495"
     CDS             complement(14286..15275)
                     /locus_tag="VNT12_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWI44614.1"
                     /translation="MQIRVDIRVPVGVPMPELVEFVKRCEDAGFYGVGIHDHHHSGRD
                     AYMALALAASSTGRICLYPATSNTVTRHPLVLAALANSLEEVAPGRTLLTLAPGFLSV
                     ESAGKRRAPLEKLRKDTTTVRRLLAGETVYSSGTPLHLRNRPERPPLVLLTASGPRLL
                     ELAGEVADGALMLVGLHPDSVAAARERLAAGANRSGSGSEKLQEIFIVPVGVGAREEV
                     REWPRRWFREGQRWLEYPSRSNLHWLKAAGIELPEELDPKSISDDLADRICDALGLFG
                     EPEYCAERLMRAQEEAGVEHVFLFPAHTFQTGYEMPGAEVETFGRVIGPRLAG"
     gene            complement(15333..15500)
                     /locus_tag="VNT12_00500"
     CDS             complement(15333..15500)
                     /locus_tag="VNT12_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44615.1"
                     /translation="MDATASSSTAQRAVKAGMVAMSKLAVPAQTVSCPWAKSTVKALN
                     ARKPASGGRVP"
     gene            15630..16073
                     /locus_tag="VNT12_00505"
     CDS             15630..16073
                     /locus_tag="VNT12_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44616.1"
                     /translation="MGEIERQSREPEGLDYVKVSLGLVFVFGAFVFGILAPPGTLVVS
                     FGLMIPAVALLASALQEANKRRRALPLASDKERELLSAIRDNGGVITPTEAAMETSLT
                     VKEADRMLSELAGGGHLSVESAGGTLCYSLPGRQATELDEPSSAT"
     gene            16234..16716
                     /locus_tag="VNT12_00510"
     CDS             16234..16716
                     /locus_tag="VNT12_00510"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWI44617.1"
                     /translation="MLEREREITLRFLAEPTDVNFGGKVHGGAVMKWIDQAGYACAVG
                     WSGQYCVTVYVGGIRFHRPVLIGNMVEVSAKLIYTGKTSMHVAVEVNAGDPKEGLYTK
                     TTQCIIVFVAVDDRGTPVEVESWEPKSEQDIALEQYAKKLIELRKDTEKEMGPHGIVL
                     "
BASE COUNT         3018 a         5625 c         5076 g         3089 t
ORIGIN      
        1 agagcagaac ggcgtcggcg gcggaagcaa gagcctttaa gtctccaacc ggcctggcgg
       61 gggtgtcgtc gagcctggag acagcttccg cgcttgtatc gtggaccagc acctcgtacc
      121 ctgccaggag cagatgtctt actactggct cgcctatgtt gccgagcccg acacaaccta
      181 gctttggtgt catagtttcc ctccgatggc atttcgagcg gcagagcgac tccccctgac
      241 cgagggtgca gggggatcga agagaccgcc gagatctctt atgatggcct ctagagccgg
      301 aggggcttgg gtgaaagatg gttggctgtc catcgaaccg ctccaccagc atccaaaaag
      361 cctccgttgc ccatcgccca gcctccctaa tcatatatcc cctccactag gagaaggctt
      421 gttgtccggt gatctcccgc cccacgacga gcgacaggac ggtatcggtg ccgtcgtagg
      481 tatagacggc ctgcatgtcg gccaggtgcc tggcgacgtg gtactcgagt aatatcccgt
      541 tacccccgag tatgtcccgc gcgtccgcca ccacttggta cgctctctgg gcgttgtgca
      601 tcttggccag ggaggccgcc ccgatcgtca tcctgccctg cgcccggagc tgactaaggc
      661 ggaaacagag cgtctgcatg gccgtgatgt ccgcaagcat gttggcgagc ttgctctgga
      721 cgagctgaaa agacgctatg ggcctgccga actgcactcg ctccctagcg tacgcgagtg
      781 ctgactcgta ggccgatatc gcgtgcccca cggcttccca agccacgttg tggcgcgttg
      841 gggtcagcac cattacggca tctccgaagc ccttcgagtt cgccagcttg ttctccgctg
      901 gcacccgcac gccttcgagt ctgacgtctg tgttcagaac ggtgcgttga gaggttttgc
      961 cggtcgtgat cgtgacttcg aacccgggtg tgcctctctc gaccaggaag gcgcccacct
     1021 tgccgtgctc ttcacgcgcc cagatcaccg tgacgtcggc gaaggaggcg ttgccgatcc
     1081 aacgcttgga gccgtctatg acgtactcat cgccctcgcg tcgcgccgcg gtttccaaaa
     1141 gcgccgcgtc ggagccgtgg tttggctcgg tgaggccgaa ggccccgatc ttttccatcc
     1201 tcgccatcgg cgggagccac ttatccttct gttcctcgga ccccaacatc gagatcgagg
     1261 tcatagcgac cccggagtgc acggcgaaga aagtggtcag gccgccgtcc ccgcgggcca
     1321 actccctgct gaccaagccc gccgccgtag ggctcattcc tggacatccg tagccctcga
     1381 tggtagtgcc cgcaacgttg agcgccgcca tcttgggcag cagctcgaat ggaaacgcct
     1441 cattttgcca atagctattg attacaggga cgacatccgc atcacagaaa gatcgaacct
     1501 tatcccggac gtcgcgttcc tcctcggtta gcaactcgtc gagcgagaag tagtcggtcg
     1561 ccttgggcaa cccgtcgtcg accaccatga tcgtcatcct ccctctacaa aagatgtacc
     1621 gctatctctg gtgacgacgg cgaagacccg caccttctcc gtgaggcctc tctgcggagc
     1681 aatcttcctg atgggagacc gctacaccag cgggatcggc gtctaggaac ccggtcgagt
     1741 ctctgacttg ccaaccaagg gacgagagcc ccgaggagca tatccacggc tctggtgacc
     1801 tgcgacatgc gccggtaaat agggtgtctc ctcggggctg attccgtgtt tcgtcgcgtt
     1861 cctccttaag atctgctacg ggcgtccgcg tcgcgcgctg ttcgcgaacc gccattcgcg
     1921 gacacttcac tcgcggcgtg ctcccgagca gcctctacct ccggctcctc cgcgaagtcc
     1981 acgtccttcg tctcgggtcc ccagacagcc ccaataagga tcagcagggc tcctatcacc
     2041 agcaggggga gcggcgtgta cttgaagggc atgaagctcg ccagtcccga ctggtagaaa
     2101 gcgtagaacg ccggcggaat gaccgccagg ctgtagccga tgccgaagcc agaggccctg
     2161 actccggtgt ggaaacgctc gttgatgtag acggtcgcca gggcccacac ccacgttatc
     2221 agtaccgtaa ctatcgttac gagaacgatc actgcaaaga ggttctgggg ggcagtgccc
     2281 acgagcacgt agtagaggaa cgtgccgacg gtcgccatga tgacggatgc cgctatcagg
     2341 aacaggcggc gtccgattct ctggccgata acgcccgccg cgatgtaggc tgctgcgagg
     2401 ataacgtagg cgatgaccag cgtaatcgta aggttggttg gggacagctt gagctcggag
     2461 gtgagtagac ccggtagcat ggccgctgcc gtgtacagcg tcagccagaa gccactcatc
     2521 atcacgaaga cctgcaggaa gctcgacaga ttcggtccct tgaataactc catgatgggg
     2581 gatctgccgc ccccttcgga ctcggactcg gcggccgctt cgaagacctc cgactcggcc
     2641 acggaggtga catagtagta gacgagggcg aaggccatca gggcaccgat gacgaacggt
     2701 atcctccagc cccactgcac gtagggggag ttcagacccc cggccggtat taattgaacc
     2761 aagatcaggg tgatcaggga gatggtggcg tacgccagcg ggtagccggt catgatcaac
     2821 gccccgaaga aaccccgctt ctcctttgga gagtactcca tcgcgagcgg gctcgccgcg
     2881 gtgtactccc caccgaggaa gatgccgtcg atgaagcgca ggaggatgaa aataatggct
     2941 gccgcgattc cccactgcgc ataccccggt aagaaggcga taagcagcgt cgccaccccg
     3001 aagccgctaa cggcgataat cgtcgtgcgc cgacgcccta tcgtgtcccc taagtgtccg
     3061 aagataaaag ccccgatcgg ccgcccgagc agcgtcgccg cgaagatcga accgttcacg
     3121 atggcggtcg tggcctcgtt catctgcggc gagacgaagt agattatggc cggggccagc
     3181 accacgattg gtaggtagat gtcgaacatg tctacgaaga accccatcca ggctccccgt
     3241 acggcgtgtc gtgctcgggg gcttagatct cctaaccgcg cgcttgctgt gcttgacatg
     3301 cactcctcct tccgtcgctt tactgtggct aacgcgcccc ttggttccct ttgttattta
     3361 ttgcgctatc gggcgcttta taaacatggg tagtgcttgg cacgagctgg tcgacgatcc
     3421 tcgcgttgag tacttcgggg cggttgagcg taaggtaccg tacggcccgg cgctgctcga
     3481 cgatcagggc cgaaccgtcg cgttgcccgt ccatgccacc cccttctcga cgagtgcgtc
     3541 tatctctttc tccgtgtagc cggcttctcc caagactgcc cgggtgtcgg ccccgagcat
     3601 gggcgccggg cggtgatgct ttccgttcga cccgagctgc caggggcttc caacggtccg
     3661 caccgtgccg gccgtggggt gctcaacctc gacgatgggg ctgcgggcct ccacctgcgg
     3721 gtcctcgatt acctcagaga ggaactgtat agcctggcac ggcacagccg cctccccgag
     3781 tcgctcgagc cagtacttcg ccggacgctt cagtagccgc tcctggacct tctcgatgac
     3841 ctcgccgcgc cgctcgcgtc ggccagcgtt cgtcgccagc ccaggatctt cggcgaggtc
     3901 ttcgagtccc agagcctcgc agaagcgaac ccacatcttg tcgttgccaa ccgccactac
     3961 tatgggcctg tcggccgtct cgaggggttg gtagacggct agaaccgagt ccgtgccgcc
     4021 gctgggccgc ggctcgggtt ctccggccag gtagctggcg atgcgcggag ccatcagcgc
     4081 gaggtcggcc tcgagcagcg agacgtccac gagctcgccc tcgccggtgc gctgccgccg
     4141 aaccagggtg gaggctacgg cgaaggccgc cgccatcccc gccacgatgt ccgagagtgc
     4201 cgtgcttacc cgctgcggcg tcccgccgaa ctccccggtc acgctcatca gccccgatct
     4261 ggcctgggcg atcaggtcat agccgggacg atcgcgatcc ggcccgtcga gcccgaaacc
     4321 cgacagggcg cagtagataa ggcgcgggaa gcgggcacaa atcgcctcag ggtcgaggtc
     4381 gcggcgcttt agcttgcctg ggttgaggtt ctcgacgaag acgtcggcgc catccagaag
     4441 gcgcatgagc gccgcgaagc cctcttctct tgagatgtcc aggcagacgc tgcgcttgtt
     4501 gcggttggca gagaggaacc aggccgcctc cccgccgacg aacggcggcc cccagtaccg
     4561 ggcgtcgtct cccgtctccg ggcgctctac cttaacgacg tccgctccca tgtcgccgag
     4621 atacatggtg gcggtggggc cggcgtacga cgtcgatatg tccaccaccc gcactccgga
     4681 gagaggccct ttagaagtcg cggcgctcac gcggaggctc ccgtggtcag caccaccttg
     4741 cccaggaccc ggctgtcccg gatctcctca agcgcctcag cggccgcctc cagcgggtag
     4801 gtagccgtga cctgcacccc gatcttcccg tgggtgagaa gaccgagcaa ctcttcctgg
     4861 gcttcccgga atgactcggg ctcgcgctcc cggtagtcgc tgctctgcac cccgatcaga
     4921 tccatattct tgaccagcac gtggcctgcc ttcacggtag gaatctcccc cgccgcgaac
     4981 cctatgatca cgagacggcc ctcccacgcg atcgcccgca tgcaggcgcg gaacagctcc
     5041 ccacctaccg actccagaat cacatccgcc ccgtgcccgt ccgtgagttc catcacccgc
     5101 tctttgaagc cttcaggctt gaactcgatg gtatgatcgg cgccctgctc cagggcgaag
     5161 ttcctcttgt cctccgtgcg tccgaccgcg attaccctcg ccccgagcgc cttggcgagc
     5221 tgcacccctg ccgagcccac cccgccgctg gcgccggtca cgagtaccca ctcccccggc
     5281 tcaagtcggg cgcgccggag taggccgaag tagacggtca ggtagcccaa gccgaaggcg
     5341 gcccccgcct cgtaccctac gctttcgggg agcacggcca cgttgttcgc ctcggccagt
     5401 acctgctcgc ggtaggcgcc gtgttcgacc tgagccgtca cccgctcacc gacattcgga
     5461 cttgcgaccc ccggcccgac cgccctgacg acccccgcga cctccttgcc cgggctgaag
     5521 ggcagctcgg gcagtatctg gtatgtaccc tcgataacca agaggtcggg gaagttgacg
     5581 ccgatggcgc gggcatcgat gaggacttct ccctgacccg gtacaggcgt tggcaggtcc
     5641 tccagcgaca tctgctccag cgcgccgaac tgcttcacca acatcgcacg cacggtcagc
     5701 cgccttcctc caatgaagcg ttgtcttctc ctagggccgg gtagccgagg cggagctccg
     5761 gccggcggaa acccggcacg acgggaagcg gcagcgcagg aatctcccgg ccatccccgt
     5821 cctcgacgga gcggtcgaag acgccccggg ccttaaagtg ctcgtcctcc agcgcctcgc
     5881 ggacgccgcg caccacggtg cagcacacgt cttcaccctc gaagcgctcc cgccaatggt
     5941 ctgcgggaag cgaggcgatg cgctcggcga tggcagtctt tgtcgccccg gggtctctcg
     6001 agtcgtcccg cgaggtttcc tccaacccca ggatgtcgca gaagcgccgc cagaacttct
     6061 cctccaaggg cgcggcggcc acgtagtgcc cgtcggccgt gcggtagatg ttgtagcgcg
     6121 gtgagccgcc ggtcagcaga tcgttgcccg gctccggcca ctccccaacg gccgtaccgt
     6181 tgcctatggc ccagtagaga aaggtgaaga cattctcgct catcgaaacg tcgaggtacg
     6241 cgccctcccc tgtcctctcc cgctcgagca gcgcgagcag gatattcatc accgccggat
     6301 aagccccgcc gcctatgtca gctatcaacg cctgcggcag cacgggcgcg ccgtcggggc
     6361 cggcggtgag cccgagcaga cccgtctccg acacgtagtt gaggtcgtga gccgccacac
     6421 cggcccgcgg gccctccttg ccgtagccgc tgatcgagca gtagaccagg cgcgggttga
     6481 tctcggccac cgcgttgtag tccaagccca gccgtttcat cactcctgga cgaaactgct
     6541 ccaccaagac gtcggcacgc tcgatcaacg gtcgcaataa cgcccaggcc tccggcgact
     6601 tgaggtcgac ctccaggctc tccttaccgc ggttcaagag agcaaagttg acgctgtccg
     6661 taccgaactt gggctcgtaa gacctcatct cgtcgcctcg accgtgacgt tcgatcttga
     6721 tcacccgcgc accagcctcc gcaaggatca gagtcgccaa cggccccggt aacagcgtgg
     6781 tgaagtctag aacccttgtc ccctccagcg gagtaatcgc cacgcctcct ctctttccac
     6841 tttacaccta tagtgtacta gcagattttt ctctgctagt caacagttgg acaattgcgt
     6901 gcgcaggcca actgtagaac gcaaggggtt ggcgttttgg gttccggctg cgggatgccc
     6961 aaatcttcag gctttgagcc gagaattcgg ctcttccctt ttacggcgcc gcttcgtaaa
     7021 ggcggttggg ctcatgcggc gggcaagggg ttcgtgtgcc tttcgcttct aggaggaccc
     7081 tcgcttgcgg gagccgttcg aggtcggcgg ttcgtcgcgc acgtcgcgca gtttttgcag
     7141 gtaattgagg tgtttctcca tcgcagccgc ggtggcctcg gcatcacttt cgcttatcgc
     7201 tttcactatg gcggcgtgct gtcgcacggt gtccctgccc gctttggcgt cgaggtgctt
     7261 ggcgaggacc ggacggatcg cctggtggag agccgtcaca aaggaggcca agacgcggtt
     7321 gcccgaagca tcgccgatag cagagtggaa atctatgtcg agtcggggga cgtcgggatg
     7381 atcgaaggta atctccttct gccggtccac gatggtccgt agcgtctcga cgtcctcctc
     7441 cgtgcggttc gcggcggcta acttcgcgga gggcacctcc aacaggtagc ggacctgcgc
     7501 gacctcgtcg tactctaccg tgccaagccg gagggtgttc actatcgagt ccccgagcac
     7561 gccgctcaag gagcggtagt ctattacctg cacgaagcta ccacccgagg ccccgggcac
     7621 cttgaagatc aggccggcgg ccgcgagcga gcgtaaggcc tctcgaatcg tggtgcggct
     7681 gacggcgaag ctctcggcaa gctcggcctc gctcggtagc ttctccccgc gcttaaaggt
     7741 gccggagaga atggcctctc ggatctgcgt ctccacctgc tcgcgggccc gggcgacggg
     7801 ttggggcttg aaagtcatac tctccgtttc ctagctcgtt aatgtcattt gaatcatatt
     7861 tatgtttgta gtcgcttgca aggtcgaggg ttacgaagcc gcgcaaggag ggccgctctc
     7921 tggtcgtgtc tgccccgttg cggagttcgg ccccgagcgg gcacgatccg gaggcaccac
     7981 tctggcctct cggtttccat gctaccgcgc atgctcgtcg ggtcgctgtt ccgcgctcgg
     8041 cgcgagccgg ttgcggatct cgaacttgcg gatcttaccg ctctgggtct tgggtagctt
     8101 ttccatcagc tcgaggcgct ccggcaagaa gtgcttggag aggccctcgc caagcaagta
     8161 ttccgtgacg tcctcgaggg cgagatgctg gtccggctcc agcaccacca cagcgcaggc
     8221 gcgttcgccc aaacgttcgt cggagacgcc cacgacggcg acgtctgcga tcgccgggtg
     8281 ctcgtacagg aggttctcca gccggtggac gggtatgttc tcgccgccgc ggatgacgat
     8341 atccttgctg cggccgacca acgacacata gccgtcctct tccatgatcg ccaggtcacc
     8401 ggtttggaac cagccatcct cggtaaacgc ctccgcagtg aagtccggcc gggcgaggta
     8461 cccggcgaag agcccgggac cccttatctg aagatcaccc atctggcctg gtggcgccgg
     8521 ctctcccgtg tcttgcacca ccctgacctc gcaaccttcg acgggtaccc cgtcggtctt
     8581 ctccaccctc tccagcggca atctgggcga tccggagatg ccgatccccc actccgtcat
     8641 accccacgcc gggcagatga agcagcccag catctcgcgg gcctgcggga ccagagagcg
     8701 agggatgggc gcgccaggga tagagatgat gcgcagcgag gggcaggtct cgggatcgat
     8761 cccgggatgg cgcacgagat cctggaggaa ggtgggggct ccgatcatcg cggtgacgcc
     8821 ctcacgcttt atcacctggg caaccacctt cggctcccaa cgatcaagat aaaccgccgt
     8881 gccgccgagg agagcggtca tgagcacgcc ccagaggaaa ccggtgtggt gtcctacggg
     8941 ggaggggatc aggttcaccg ggtcccggtt gagcatctct tggccctggt gctccaccca
     9001 gcggcggcac acgaagtcca gcatctggtg tgtattcatt acgcccttgg gctcaccggt
     9061 ggttccggag gtgaagccga catgggccag cgaacccggg gctaccaccg gcgggaggtc
     9121 cagcggctcc gcggaggcga gttcctcgaa ggacagccat cccggctcct ccggcgcttc
     9181 gtcgcccaac accacgacgt ggcctagctc cggtgtctcc gataacagcc tcccggccat
     9241 cgcaacgtgg tccgtccggc tttcgccgga cgggatgacc aagaccttcg ctcgcgcgcg
     9301 ccacagcata agggccgcgt ccttctcccc atagcttacc gggatgccgg aatagaccgc
     9361 gccgaggcgc gagatcgcga agatgagcgc accgaactcc agtcgattcg gcaacatgag
     9421 cgacaccgtg tccccgggcc cgacaccctc gcgggaaagt ccacccacca ggcggtccac
     9481 cagctcgttc agctcaccgt agcttgccga tgccgttcgc tcccccgtct gtccgtcgac
     9541 cgacacggct gccagtttgt ccggccccgc cgcggtagcc gcggcaaggt agctgtcgag
     9601 ggtcacctcg cgccacgccc cctccttccc gtagcgtgtg accaactccg gcgtgagcct
     9661 gctctccggt atcgcgacaa gctcttcgct cgtgaacatg ctcaacccac ctttcccctc
     9721 cggcaacctc accggaatcg ttcatcgttg gatcaggcac tagccccatc gacagcacaa
     9781 tgtacgacaa aagtaaatat atctctatta gaccttatag tcaaatatca tacaaaaacc
     9841 ttgcctggta attcgaaacg cacgcgccga gttagacctc tccttcgcca ccaggaagaa
     9901 ggataagtcc gggcacccgg gcgtggagcg ccggcctaac ggttgaaacg gtcactgtcg
     9961 ggcggtcggc gctctgagaa ggccgcggag agctccttcg cctcctcggt cctcaggtac
    10021 tgcgtgagca gcaggtcgtg cgccatgcgt gcctgtccca agacccccag atgccggcct
    10081 gcgaaggccg tcttgagcgc ggcgagggcg tgcgggccgc gggagatcat ctccccggac
    10141 agctcccaga cgcgctccat tagcctctcc tgcggcacca cctcattgac gaggccaatc
    10201 gccagcgcct gctcggccga gtacttgtgg ttcaagtacc acatctcctt ggccctcttg
    10261 cgcccgatgg cctcttcgag gtaccaggtg ccgaagcccg cgtcgtagct gccgaccatc
    10321 gggcccacct gccggaagac ggcgttctcg gaggcgacgg agagatcgca catggtgtgc
    10381 agcacgttgc cgccgccgac ggcgaacccg ttgacggcgg cgacgaccgg cttggggatg
    10441 gtgtcgagca actcgtagac gtccactatc ggcagcacga ccgagtggtc atatccctcg
    10501 aacggttcgt gctcgccgcc gatacagaag aacctgtcgc cggcgccggt gaggatgccg
    10561 acccgcagct cggggtcgtc gcgcagggag cgcaacgcct cggcgagctc tagcgtggtc
    10621 ttacggcgga acatgttgcc tttatccggg cggttgatgg ttatggtggc tatgccacca
    10681 tcgtggtcta ccctgagatc ttcgaagctc acgagcgtcc tccattaccg tcgctgtagt
    10741 catgaaaacc gcgtccggac ttgcgcccta ggtagcctgc gtccaccatc ctgctcagga
    10801 gtggtggcgg ggcgaggtgc ggatcgtggg tctgctcgta ggctgcagag gtcgcgtaca
    10861 gatggacatc caacccgata aggtccagaa gcttgaggcc gcccatgggg taacccaagc
    10921 ctccctcgag cgccacgtcc atgtcctcag cggtggcaag gccgtcgtcg tacgcctgca
    10981 caacctggtt taggtacggc atgagcaggc ggtttaccag gaagccgggc cggtccttgg
    11041 tacgcacggg ttccttgccg atctccttcg caaacgactc cagaaagtcc agggtctcct
    11101 cggaggtctg caccgcggcg acgacctcga cgagcctcat gagcggggcg gggttgaaga
    11161 agtgcaggcc cccaaaacgc tcgggacgag gtaccgtagc ggcgagttcg gtcaccgaaa
    11221 gcgccgacgt gttggtcgcc atgatcgcgc cctcccccac cacatcggcc accacgggga
    11281 gcagtgcccg cttggtttcg aggtcctcga ccacggcctc gatcaccacg ccagcgccgg
    11341 cgagatcgcc gacctctgtc gtccctcgca cccgcgctag cgcctcgttc cgctcctcct
    11401 ccatcacttt ctctcggcgc acgccttcgc caagaaacgc ctctagccgt tggcgcccat
    11461 cctcgaggcg ctcctcggac tcttcgagca ccacgacctc gctcccggcc agggcgcaga
    11521 cctgcgctat ccccgcgccc atcgtcccga agcccaaaac ggcgacgttc ccgttgtcgc
    11581 tcatctctcc tcccagcagt gcatcggtgg tgtcattccc cctattcgta ctcgtaaaat
    11641 cccttgcccg tcttgcgccc aagatggttc gcagagatca tgcggtccac gagcggcggc
    11701 ggggcgaagc gtgggtccct aagctgctgg taaagcgaca tagagacggc cttgtggatg
    11761 tcgagcccca cgatgtcgag gagcttgaag gtccccattg gatagccgag ccccagggtc
    11821 acagccttgt ctatcgactc gaccgtgccc atgccagcct ccaaggcgcg gatgcagtcg
    11881 ttctcgaagg gaacgaggaa gtagttgagg atgaagccgg gagagtcctt cgtgaacacc
    11941 acccgcttgc ccaagccctc gacgaacgcg accgacctcc gtagcgcctc gtcgctcgtg
    12001 agacggccgc gcgtcacctc cacgaggtca acgagcggcg cggggttggc gtagtgtgtg
    12061 cccacgaccc gctccgggtg cgtggatccc gccgcgattc cagtcacgga taaggtcgag
    12121 gtgttcgtgt ggaagagcgc ctcggggccg cagatctcgt cgagttggcc aaaggtctcg
    12181 gccttcaggg cctcgtcttc gaagatcgcc tcgatcacca cgtcgcagcc ggcgagcgcc
    12241 tctatctccg ttgtccccga ggtgctcgcg agggcctcgt cgcgcctttc ggcgtctagc
    12301 ctgcccctct cgacgctgcg atcgaggaac tgcttgaccc gggcaagtcc atcttccacc
    12361 cgctcttgag agatgtcccg tacgaccacc tcatgccccg cacgggcgac ggtgatggcg
    12421 atcccactcc ccatcgtccc cgccccgaga ataccgactc ttagccggtc cttatcccgc
    12481 gcactctcag caggctgggg ctctcgactt cccgtctcca ctctaaccct ccgtttagcc
    12541 taggcctaca gtaataatgc tcgacatttt ccttgatgtc aagcattgga ccatatgctg
    12601 tttctatacg caccaccgtt cacgccgttg tctcccctcc tcgcaagcgc tacaacggcg
    12661 ttttgaggcg ctggccctcc ttgcggcgtc ccggtatttc gggacctttc gctagccgaa
    12721 ggctctggct agggcggtgg tcgccaccgc gagggtgact gcaaccagca ccggcgcccg
    12781 aagcgagaga gcgaccgccg caacggctac ccccatggcc ttctcgtcga gtaccagccg
    12841 cccatcctca ccaaaggtct gggtgagtac cagtgccgtg aggagggcgg gcaccagcag
    12901 ggcgatgacc gcgcccatgc tcggaggcag ctcgcgacca cccaccagga tggggccgag
    12961 ggccctgata gctgcgctgg ccaaagccac gactacgata gtaatccaaa gcgccgtcac
    13021 gacgatcgcc ccctcaagcc gaggagcgct gcggcgcagg aggcgatcac cggtatgccc
    13081 gggggcgtga acgggaccag cgcgagcgac agcgccgccg cgatcagcac cacggcgatc
    13141 gtctgcccac cagcgcgcag ttcctccacc agcagggtga ggtagaaggc cgggaagatc
    13201 acgtccaacc ccaaccgctc gacgttcccc acgacacctc caacgagtac acccacaacc
    13261 gtcccaacga tccacaaagc gacctgcggg atggtggcgc ctatcatgaa ttctctatcg
    13321 aagcgcccgc ctccccggct ggccatagcc caggaggcgt ctactaccgc ctggccctcc
    13381 aaggcccggc gcaagggccc gcctttaagg aacggggctg ccgcgatccc catgggcaaa
    13441 aagcgagcgt tcaacagcac agcggcgaca atcgccggca ccactccacc gcccccgccc
    13501 aacaccgaca ccaccgctaa ctgggcggtc ccggagaagg caatcaccga aaagaccatc
    13561 ggagcgacca cgccccaacc caacgagcgg gccaaaaccc cgaaagagat ccccatcact
    13621 cccgtcgcca tcgcgagcgg cagcaccgcc cgcgcaccgg caagatagac tccgccgccg
    13681 cattctcctt cgctactcat gctcgtactc tcacctccca cggttgttaa tggtcaaagt
    13741 acgttatgag tattaagact gtacccgttc tcgttaatgg tcaaacactg ttatactcat
    13801 tagtaatcgt agagatcgtc tgaagccggg aggcgaacgt gagtagcgtc agacgagaga
    13861 ggagattcaa gtttagaggc ggcgttccat gcctggattt cgtcaacact ctggcttggc
    13921 gccttaccga ctgccctgtg gagtacctac tctcctacga ggatctgcta gcgtggggaa
    13981 ggcaggcgag tctgctcaca ctggatgaga aggaggtact ctctagatgt gcggcgatgg
    14041 atcccgagga ggcacggggg acgctatcgc gagcgttcgc cctgcgggag gcggtccatg
    14101 gggtgctctc cgctgcgatc gctggcgagc cccaggacga ggacgccctg gcggcgctca
    14161 accacgaact ctcgggggcg ctctcgcggc tgcgggtggc gccggtggca ggcgacacct
    14221 acgtctgggc ctgggagcgg ggcgggtatt gccaggtttg tgtccacaaa tccttgatgt
    14281 gggactcagc cagccaagcg gggaccgatc acccgtccga aggtctcgac ttcagcaccg
    14341 ggcatctcgt agccagtctg gaaggtgtgc gcgggaaaga ggaagacgtg ctcgacccca
    14401 gcttcctctt gagccctcat cagccgttcg gcgcagtact ccggctcccc aaagaggccg
    14461 agggcgtcgc agattcggtc cgcaaggtca tcagagatgc tcttcgggtc tagttcttcg
    14521 ggcagctcta ttcccgccgc tttgagccag tggaggttcg agcggctcgg atactcgagc
    14581 cacctctgtc cctccctgaa ccaccggcgc ggccactccc tgacttcctc tcgcgcaccc
    14641 acgcccaccg gcacgatgaa gatctcctgc aacttctccg aaccgctgcc gcttcggttc
    14701 gctccagccg caaggcgctc acgggctgca gcgacagagt ccgggtggag acccaccagc
    14761 atcaaggccc cgtcagcgac ctcccccgcc aattccagca aacgcggacc cgaggctgtc
    14821 agtaacacta gcggcgggcg ctctggtcgg ttacgcagat gcaggggtgt cccgctggag
    14881 tacacggttt cgccagccag cagccggcga acggtcgtcg tgtccttccg cagcttctca
    14941 aggggcgccc tgcgtttgcc ggcagattcg accgagagaa accccggtgc aagagttagc
    15001 aacgtccttc ctggggcgac ctcttccagg gagttagcca aggccgcgag caccagcggg
    15061 tggcgcgtaa cggtgttgga tgtcgccggg tagaggcaaa tccgacccgt actcgaggcg
    15121 gccagggcga gagccatata agcgtccctg ccgctgtggt gatggtcgtg gatccccacg
    15181 ccgtagaaac ccgcatcctc gcaccgcttg acgaactcga ccaactctgg catcggcacg
    15241 ccgaccggta cccgtatgtc taccctgatc tgcatgctcc catcctgcat cgggtcgaac
    15301 cctctttccg cgctgtgctt gccctgatcg cctcagggca ctctaccacc gctggcgggc
    15361 ttcctggcgt tcagcgcctt caccgtgctc ttggcccacg gacagcttac cgtctgggcc
    15421 ggtacggcga gtttgctcat agccaccatc ccggccttca ccgctctctg ggcggtgctg
    15481 ctgctggccg tagcgtccat gccgctcgta cgtgaccttg gtccccgcga accacgccgg
    15541 tcgcgctaga gccccgccac cttcgaactg ttgagctaga atgttcggca acgtagaaga
    15601 gaacgagacc atggttgtga aggagagagt tgggtgagat cgagcgccag agccgcgagc
    15661 cggagggcct cgactacgtc aaggtgagcc tgggcctcgt cttcgtcttc ggcgcgttcg
    15721 ttttcggcat ccttgccccg ccgggcacgc tggtggtctc cttcggcctg atgatccccg
    15781 ctgtcgcgct cctcgccagc gccttgcaag aagccaacaa acggcgacgt gcgctcccgc
    15841 tagcctccga taaagagcgg gagctactgt cggccatccg ggacaacggc ggcgtcatca
    15901 cccctacgga ggcggcgatg gagacctcgc ttaccgtgaa ggaggccgac cggatgctct
    15961 cggagttggc cggaggtggg cacctctccg tagagagcgc gggcgggacg ctctgctatt
    16021 cgctccccgg cagacaggcg accgagctcg acgaacccag ctctgctacc taacacaatc
    16081 cgcccccgcc agcgcctccc ccgcgcagca gaataggacc cgacggttgg gcgctagggc
    16141 gagccgaagt ccttaggcta ggggtctccg acttttctcg gcgagcttac ggaaggtgcg
    16201 cggcctagct ttgcccagta gaatgccccc gctatgctgg aaagggagcg agaaatcacg
    16261 ctgcggtttt tggctgagcc gaccgacgtg aacttcggcg gcaaggtgca cggtggcgcg
    16321 gttatgaagt ggatcgacca agccggatac gcctgcgcgg tcgggtggag cgggcaatat
    16381 tgcgttacgg tttatgtcgg tggtatccgt ttccacaggc ccgtactaat cggcaacatg
    16441 gtcgaagtga gcgccaagct tatctacacg ggcaaaacga gcatgcacgt agcagtggaa
    16501 gtgaacgcgg gcgacccgaa ggaaggtctg tacaccaaga ccacgcagtg catcattgtc
    16561 ttcgtggcgg tcgacgatcg aggaacgcct gtcgaggtgg aaagctggga gccgaaatcg
    16621 gagcaagaca ttgccctaga gcagtacgct aagaagctca ttgagttgcg gaaggacacc
    16681 gaaaaggaga tgggccccca cggcatcgtg ctttaaggtt gtcgcgccta cctagccttc
    16741 gcctcctcgc gggcctcgct cctatgtgat aagccgaacg atcggcgcgt cgctggggtc
    16801 gaggcaga
//
LOCUS       DATMJA010000022        13574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_595743, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000022 DATMJA010000000
VERSION     DATMJA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595743"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..210)
                     /locus_tag="VNT12_00515"
     CDS             complement(49..210)
                     /locus_tag="VNT12_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44618.1"
                     /translation="METKNGQTILSGEVKDQPHLFGILERLNSLGLELLSVQALKEDA
                     HPIAEVIES"
     gene            complement(297..968)
                     /locus_tag="VNT12_00520"
     CDS             complement(297..968)
                     /locus_tag="VNT12_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWI44619.1"
                     /translation="MNNLDLRGEIVQVSRRMSTSGLVVGTSGNVSARTPDGNVLVTPS
                     GLDYAILEPEDVVLVDLDGKILEGSFEPSVETPMHTGIYRARPEAGGIVHTHARYSTT
                     LACLNWEILPIHYMLAVLSDEGRVPIAEYATYGTEELARNASEVLGSSHRSCLLRNHG
                     TIAIGASVREAYARTELLEEMAEIYYRARVAGEPVLLTPEQMAEVSAKIHDYGQSKPS
                     PAEIE"
     gene            complement(988..3084)
                     /locus_tag="VNT12_00525"
     CDS             complement(988..3084)
                     /locus_tag="VNT12_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HWI44620.1"
                     /translation="METRIPGATENLWDPQRAQGLSELETLTYRSNLLGTDRAVANFG
                     GGNTSTKARERDHAGREVDVLWVKGSGSDLATIKAERFTGLKLDEVLPLEEREEMSDE
                     EMVAYLASCQLRPDMPRGSIETLLHAFVPYAHVDHTHPDAVNMICCAKGGEELAAECF
                     GEDAVWIDYIRPGFTLSKQVGKAVRSNTNARFVLLAKHGLVTWGKTHQESYGRTIEAI
                     NRAAEFVAQRGGEPFGGRSVKPLSPERREALLAEVLPALRGALSSGAAEVSHKILRAD
                     YTSEDVLEFVCGRDSKELSQVGAACPDHLVRTKVKPLWVDFDPESEGAEELKAMLREG
                     VEGYRREYEEYYARNRERNPQGDAPMFDPNARVVLIPSVGLIGAAHNVKEADLSRDFA
                     YRAINVMRGAYALGGYVSLTEEESYAVEYWPLELYKLTQAPPPRELAGRVAFITGGAG
                     GIGSAVAKSLAGEGACVVVCDLDEEGASEVAEGLPQPAITARSDVTDEGEVALAYRRA
                     VLGYGGLDVVVSNAGLASSAPIEETSVEMWDKNHAVLAKGYFLVAREAFRIMKEQGVG
                     GSLVFVASKNSMAAGKNASAYSSAKAAELHLARCLAEEGGPESIRVNTVNPDAVLQGS
                     RIWDSSWRTERAQAYGIEPEELEEFYQQRNTLKVNVTPENVAEAVLFFASEARSSRTT
                     GNVLNVDGGVKDAYPR"
     gene            complement(3164..4375)
                     /gene="rhaI"
                     /locus_tag="VNT12_00530"
     CDS             complement(3164..4375)
                     /gene="rhaI"
                     /locus_tag="VNT12_00530"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HWI44621.1"
                     /translation="MERTTSQAYDALAQYVSTKGVDLNNVEDRLRSQRIETPSWGYGN
                     SGTRFKVFPHPAVPRDPFEKFEDAAQVHRLTGVCPSVAIHIPWDAVEDYGELKDHAES
                     LGLRIGAVNPNLFQEEEYSLGSVCNPNPAVRRTATNHLIECVEIGREVGSDVLSLWFA
                     DGTNYAGQDAFVARRHRMQECLSEAYEAMPENMRMLLEYKPFEPAFYHTDLSDWGAAL
                     TMCQRLGERAEVLVDLGHHLQGTNIEHIVALLLDEGRLGGFHFNNRKYADDDLIVGSV
                     NPFELFLIYVELVEAEESTRIDYMIDQAHNIEPKIEAMILSVTNLQEAYAKALLVDRR
                     ALTESQLSRDVLAAHRTLLDAYSTDVRPLCAKVREDLGGSADPVADFRQSGHLEKATE
                     ERKEGAQAGWG"
     gene            complement(4447..5946)
                     /locus_tag="VNT12_00535"
     CDS             complement(4447..5946)
                     /locus_tag="VNT12_00535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HWI44622.1"
                     /translation="MSELLLGIDIGTSSSKGVLSRPDGEVVATAERPHELSLPHPGWA
                     EHDAEGVWWADFVSICRELLEEADDRIAAVCTSGIGACLLPTDEEGRPLRPAILYGID
                     TRATREIEELNERYGTETLLGRCGSVLTTQAIGPKLLWLRRNEPEVWEKARKFFMANS
                     FIAWRLTGEYVLDHHSASQCDPLYDVHSYYWIDEWAEEIAPGLPLPRLLWPAEVVGEV
                     TQQASEATGIPTGTPVAAGTIDAWSEGASVGVQKPGDLMLMYGTTMFMIEVLSEPLNH
                     PGLWGTTGIVPNTHNLAAGMATSGALTGWLKKISDDLPYERLTEEAANVRPGSDALVV
                     LPYFAGERTPLFDPRARGLISGLTLSHGRAHLYRAMLEATAYGVRHIFEFMRDAGGGG
                     ERLVAVGGGTKGGLWTQIVSDVTGSAQDLPEQTIGASYGDALLAARSIGLADPDTDWS
                     KISETVEPDEQNRGIYDELYHIYRDLYPATREQVHKLAVLQKGGRDTVA"
     gene            complement(6068..6958)
                     /locus_tag="VNT12_00540"
     CDS             complement(6068..6958)
                     /locus_tag="VNT12_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWI44623.1"
                     /translation="MMPKLVGIPYFNASEQGANEAAKELDVDLVYDGPTEAKAALQSQ
                     MIEQFIQQQCDAITVAANDPNALAPAMKKADNAGIATGAWDADVAPDARDVFVNQATF
                     QAIGYKLVDVMAEQTGGKGEFLVVTGSLTAPNQVAWLKEMRERMKEKYPEMSIASVEP
                     GEEDLQLGIDITKNYLRANPNTAGVFGITSVALPGAAEAVQQIGRAGEVPVTGLSTPN
                     EMKPYIESGAVKEFVLWNPVDLGYLAVYVANAQVEGNLPKSGTFKAGRLGEVKLLAED
                     EVLLGPPIVFSKENIDQYDF"
     gene            complement(7165..8121)
                     /locus_tag="VNT12_00545"
     CDS             complement(7165..8121)
                     /locus_tag="VNT12_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI44624.1"
                     /translation="MSALVGTMRRLKFSRELVLLGVLIALVVVMTLLDPLFLTPTTML
                     NTSRFFVEVGLMALGMTLIIITGGIDLSVGSNLALASVAVGFSFAAGLPLPLAILFGL
                     VVGVAAGLFNGLFITLLNLHPLVVTLGTLALFQGLAYGISKAQAVSDFPGWFAYFGQA
                     YLGPIPGQLFIFVMAVAVVWVILSRTSFGRYVYAIGNNEEAARFSGVPIRKVKLVLYS
                     GIGLLVGMAAVIYTSRVYTARGDSGVGLELDVISAVVLGGASIYGGSGTIGGTVLGVL
                     IIATLRNGLILAGVPSTWQLFVLGVLLLAAVFINEFFRKGDE"
     gene            complement(8118..9119)
                     /locus_tag="VNT12_00550"
     CDS             complement(8118..9119)
                     /locus_tag="VNT12_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI44625.1"
                     /translation="MSETRAPTPTERGGAGGESVRRFLARPAAPALAFLAVLLVAFTL
                     ANDRFLTAYNLESIVDSVAVLGVIALAVNQVILCAEIDISTGSMLGLCAIAAGAVSLS
                     TGGILLPLLAGVAVGALAGAINGALVTLGRIPAIIVTLGMLYALRGVILLVTGGTWIT
                     GVPEPTRFLGTGKVLGISVPVLILFGLFLVMELVNRHSTWGRNVFAVGGNRVAARFAG
                     LPVNRVRFVAFTLVGVFVGIASMIYLGRAGSVQTNTGVGLELQAVAAVVIGGTSISGG
                     RGSSLAALTGAVLIGVILNGLILMGVPGIWQDAVLGGLILLAVATDVLRRRLVGDKA"
     gene            complement(9112..10719)
                     /locus_tag="VNT12_00555"
     CDS             complement(9112..10719)
                     /locus_tag="VNT12_00555"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWI44626.1"
                     /translation="MSEEGKEIGRGMEQPTNIGSGTATGDGSPPLFQLAGVTKRFGGV
                     VAVRDVDFDLRPGEVHALVGENGAGKSTLMKIVDGLYAPDEGRLEVGGQPAEFSSPRD
                     AEAAGIAMIPQELDLFPELSVAENLFVGRKRPRTRWGGLDRNAMRAEARRWLGSLGVD
                     LDVTARVKRLSAANQQIVAIARALIGDARAVVMDEPTSSLTDREARQLFRIIEELTSG
                     DVGVVYISHRLEEIFEIADRITVLRDGRLITTAPASELDAEELVRLMVGRPLHELFSR
                     HPHDPGEVALEVRGLTREGEFAEVDLTLRRGEILGLAGLVGAGRTELAQAIFGIRPPE
                     FGEILVDGEEARIRSPQAAMDRGIFYVPEERRSQGLILPFSIKNNITLSILDRISRFG
                     FVPWSERQTADRFAKELSIRGAEMSDPVARLSGGNQQKVVVAKSLAREPSVLLLDEPT
                     RGVDVGAKSEIYRLIDDLAREGKAILLISSELEEVLSMSDRILVMREGRITGEFEREG
                     ATQERVMTAATGGGDGSNDKGEESGDE"
     gene            complement(10720..12213)
                     /locus_tag="VNT12_00560"
     CDS             complement(10720..12213)
                     /locus_tag="VNT12_00560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HWI44627.1"
                     /translation="MSARADFLAVDLGAESGRVFLGSFDGERVHLQEIHRFPNVPVRV
                     ADGLHWDVLRIVHEVKEGLARAARESGNPASVGVDTWGVDFALLDRDGALVSNPYHYR
                     DPRTEGMMERAAERVPEEEIYRTTGIQFMPLNTLYQLLAFEDSLLLEAAYTLLMMPDL
                     IGYWLTGEKACEYTVATTTQLYDLEAGDWAWELMRRMNVPERIFPEIIQPGTQLGTLL
                     PEVREGTGLGEVPVTAAATHDTASAVVAVPAEGPFAYISSGTWSLVGVEMPHPVTTEG
                     ALHHNFTNEGGFGGTVRVLRNVMGLWLLQECRRTWVRNGRDYSYEELVRLAETAPQFG
                     ALVDPDHSGFLAPGDMPARVRDFCTKTGQNLGEEPGAVARCVFESLALKYRWVLERAE
                     EVSGQPAETIHIVGGGAQNTMLCRLTADATRRPVVAGPVEATALGNVMAQAYARGYVG
                     SLQEIRDIVQSSAEVQTYAPEDKADEWDEAFGRFSELMDAPLEGAVT"
     gene            complement(12210..12572)
                     /locus_tag="VNT12_00565"
     CDS             complement(12210..12572)
                     /locus_tag="VNT12_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HWI44628.1"
                     /translation="MSEGAYVWLIPDGFFPEVSSGSQESHEAICVLNTSAEKARLSIS
                     FYFEDREPLKDVMATVPPERTRHIRTDQLGRAEIPRGVPYAIRVESSIPVVIQCSRMD
                     TTQAALSLMTTIAHPVRP"
     gene            complement(12582..13349)
                     /locus_tag="VNT12_00570"
     CDS             complement(12582..13349)
                     /locus_tag="VNT12_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HWI44629.1"
                     /translation="MIPEKRRRRIAQLVRETGGVTIALLEAEFGISPMTARRDLIVLE
                     EEGRVRRTHGGAVLPEYAGHEDSFWYRLDKAVEAKERLAEAAVGLLEPGDSVFLDCST
                     TTYYLARRILREGPQVTLLTNSVPVMELFMKNEAPKTGVVGVGGFMRKLTLSFVGPHA
                     VATISAHSTDRAFVSVKGVTREGFLTDPDVLEAEVKHAMIEHSEEPILLVDRSKFERR
                     GLSVIAHASKLSRALVVDAPEERLESLTIAEVDVRRV"
BASE COUNT         2340 a         4470 c         4185 g         2579 t
ORIGIN      
        1 ggtgaataag cctcgccggt gaggtggtca gggtcccggt taatggcttt agctctcgat
       61 cacctcggcg attgggtgag cgtcttcttt caaggcctgc acgcttagga gctccaaccc
      121 caagctgttc aggcgctcta ggatgccgaa taggtgcggc tggtctttga cctctccgct
      181 caggatcgtc tgcccattct ttgtctccat ttccatacct tcgaaggcag aggcgtaata
      241 aaaggcgatg ctcgcgccta ccactcttga cccgaccacg attactccag gcgtgtttat
      301 tcgatctcgg cgggtgaggg tttggactgc ccgtagtcgt gtatcttcgc cgagacctcg
      361 gccatctgct ccggggttag gagcacaggt tccccggcca ctcttgcgcg gtagtaaatc
      421 tcggccatct cctccagaag ctcggttctg gcgtatgcct ccctcacgga cgccccgatg
      481 gcgatggtgc cgtgatttcg gagcaggcac gatcggtgtg agcttccgag gacctcgctg
      541 gcgttgcggg cgagctcctc tgtcccgtac gtggcgtact cggcgatggg caccctccct
      601 tcgtccgaga gtacagcgag catgtagtgg atcgggagta tctcccaatt caaacaagcc
      661 agggtcgtcg aatagcgggc gtgggtatga actatgccgc ccgcttcggg ccttgcccga
      721 tagatcccgg tgtgcatagg ggtctcgacc gagggttcga aggagccttc gagtatcttc
      781 ccgtcgaggt ccacgagcac gacatcttca ggctccaaga ttgcgtagtc gaggccgcta
      841 ggggttacga gtacgttgcc gtcgggggtg cgggcgctga cgttcccgga ggtgccgacg
      901 accagtcccg aggtgctcat acgtcgagag acctgtacta tctctcctcg cagatccaga
      961 ttattcaagc gtgctctcct tctcgcgcta acgaggatag gcgtccttga ccccgccgtc
     1021 cacgttgagt acgttgccgg tggtcctgga cgaacgcgcc tcggaggcga agaagagtac
     1081 ggcctccgcg acgttctccg gcgtcacgtt caccttcagc gtgttgcgct gctggtagaa
     1141 ttcctccagt tcctccggct ctatcccgta ggcctgagcc cgctccgtcc gccaggagga
     1201 gtcccagatc ctcgagccct gcaagaccgc gtcggggttg acggtgttga cccggatgct
     1261 ctcgggtcct ccctcctcgg ccaggcaccg ggccaggtgc agctcggcgg ccttcgccga
     1321 ggagtaggct gatgcgttct tccccgccgc catggagttc ttggaggcca cgaagacgag
     1381 gctgccgcca acgccttgct ccttcatgat cctgaacgcc tcgcgagcta ccaggaagta
     1441 gccttttgcg agtacggcgt ggtttttgtc ccacatctcg acactcgtct cctcgatcgg
     1501 ggcactggag gcgaggccgg cgttcgaaac gacgacgtcc agcccaccat accccaagac
     1561 cgcccggcgg taggcgagag ctacctctcc ttcgtcagtc acgtcagacc ttgcagtgat
     1621 cgcgggctgc gggaggcctt ctgctacctc actggcgccc tcctcgtcga ggtcgcacac
     1681 cacgacgcac gctccctcgc cggccagcga cttcgctaca gcgctgccta tgccgcctgc
     1741 ccctccggtg ataaaggcga ccctacccgc aagctccctg ggtggaggag cctgggtgag
     1801 tttgtagagc tcgagcggcc agtactcgac tgcataggac tcctcctccg tgagggagac
     1861 atagcctcca agagcgtagg cgccgcgcat gacgttgatg gccctgtagg cgaagtccct
     1921 ggagaggtcg gcctccttga cgttgtgcgc tgctccgatg agcccgacgc tcgggatcag
     1981 gacgaccctc gcgttggggt cgaacatcgg cgcatcgccc tgcgggtttc tctctctgtt
     2041 gcgcgcgtag tactcctcat attcccggcg gtatccctct acgccctcgc gcagcatcgc
     2101 ctttagctcc tcggcgcctt cactctccgg gtcgaagtct acccacagag gcttgacctt
     2161 cgtcctcacc aggtggtcgg ggcaagccgc tcctacctgg gagagctcct tagagtcgcg
     2221 cccgcagacg aactcgagga catcttcaga ggtgtaatcg gcgcgcagga ttttgtggga
     2281 cacctccgcg gcacccgacg agagcgcccc ccgaagggcg ggcaagactt cggcgagtag
     2341 agcctccctc cgctcgggag agagcggctt gaccgatcgg ccgccgaagg gctcgccgcc
     2401 gcgttgcgct acgaactcgg cagcccggtt gatggcctcg atggtgcggc cgtagctctc
     2461 ctggtgggtc ttcccccacg tgacgagccc gtgcttggcg agcaggacga acctggcgtt
     2521 cgtgttgctc ctcacggctt tgccgacctg cttggagagg gtgaagccgg gacggatgta
     2581 gtctatccag acagcatcct cgccgaagca ctcggccgcg agttcctcgc cgcctttggc
     2641 gcagcagatc atgttgaccg cgtccggatg cgtgtgatcc acgtgcgcat aaggcacgaa
     2701 ggcgtgcaga agcgtctcta tggagccgcg aggcatgtct ggccttagct ggcagctagc
     2761 gaggtaagcg accatctcct cgtcgctcat ttcctcacgc tcctcaagcg gcaggacctc
     2821 gtcgagctta agacctgtaa accgctccgc ctttatggtc gcgaggtcgc tccccgagcc
     2881 cttgacccag aggacatcca cctcgcgccc ggcgtggtcg cgctctctgg ctttggtcga
     2941 agtgttaccc ccgccaaagt tggccaccgc gcggtcagtg cccagcaagt tggagcggta
     3001 ggtgagcgtc tccagctctg agagaccctg ggctctctgc gggtcccaca ggttctctgt
     3061 ggctcctggt atcctcgtct ccatgttttc tgaaaccttc ctcttgtcgt tcgcatcctt
     3121 attctgggcg acaatcgaat gccgcccact atactgtgtg gggttaaccc caccctgcct
     3181 gcgcgccttc tttgcgttcc tctgttgcct tctccagatg tccgctctgg cgaaagtcgg
     3241 ctacgggatc agccgagcct ccgaggtcct cgcgcacctt ggcgcaaagc ggccgtacgt
     3301 ccgtgctgta ggcgtccagg agggtgcggt gagcggcgag gacatccctg ctgagctggg
     3361 attcggtgag agctcgacga tccacaagga gggccttcgc gtaagcctcc tggaggttgg
     3421 tgacgctcag gatcatggcc tctatcttgg gctcgatgtt gtgggcctgg tcgatcatgt
     3481 agtcgatgcg ggtgctctcc tcggcctcga cgagttctac gtagatcaag aagagctcga
     3541 acgggttgac ggaacctacg atcaggtcgt cgtcggcgta cttgcggttg ttgaagtgga
     3601 agcccccgag cctcccctca tccagcaaga gcgccacgat gtgctctatg ttcgtgccct
     3661 gcagatgatg ccctagatcc actaggacct cagccctctc gccgagcctc tggcacatgg
     3721 tgagtgccgc accccagtcg gagaggtcgg tgtggtagaa ggccggctcg aagggcttgt
     3781 actcgagcag catgcgcatg ttctcgggca tcgcctcgta ggcctccgag aggcactcct
     3841 gcatgcggtg gcgccgcgcc acaaaggcgt cctggccggc gtagtttgtg ccgtcagcga
     3901 accacaagga gaggacgtcg gagcccacct cgcgcccgat ctcgacgcac tcgataaggt
     3961 ggttggtggc tgtgcggcgc actgcggggt tggggttgca gacgctcccc aaactgtact
     4021 cttcctcctg aaacaggtta gggtttacgg cgcctatccg cagtcccagg gactcagcgt
     4081 gatccttcag ctccccgtag tcctcgaccg catcccaggg aatgtggatc gccaccgaag
     4141 ggcacacgcc cgtgaggcgg tgcacctgcg ccgcgtcctc gaacttctcg aaggggtcgc
     4201 gggggacagc cggatgtggg aacaccttga agcgcgtgcc ggagttgccg tagccccacg
     4261 atggtgtctc tatcctctgc gagcgcagac ggtcttctac gttgtttagg tctaccccct
     4321 tggtcgagac gtattgggct agtgcatcat aagcttggct ggtagttctt tccaaagctc
     4381 tttccttctc cgcgtcggcc ttccaagttc acttcaggtc actttgcgta gaacggtctg
     4441 ctgtacttag gcgactgtat cccgtccccc tttctgcaag acggctagct tgtgcacctg
     4501 ctcgcgggtg gccggataga gatcccggta gatgtggtag agctcgtcgt agatcccgcg
     4561 attttgctcg tcgggctcga cggtttcgga gatcttgctc cagtcagtgt cgggatcggc
     4621 caggccgatg gaacgcgctg cgagcaaagc atccccatag ctagctccaa tggtctgctc
     4681 gggcaggtcc tgagcgctgc cggttacgtc ggagacgatc tgggtccaga gtccgccctt
     4741 cgttcctcca ccgaccgcta caagccgctc tccccctccc ccggcgtcgc gcatgaactc
     4801 gaagatgtgg cggacaccgt aggcggtagc ctccagcatc gccctgtata agtgggcgcg
     4861 gccgtggctc aaagtgaggc cggagatcag tccgcgcgcc cgcgggtcga agaggggggt
     4921 ccgctctccg gcgaagtacg gcagcacgac gagggcgtcc gatcctggcc ttacgttagc
     4981 cgcctcctcg gtgagcctct cgtagggcag gtcgtccgag atcttcttga gccagccggt
     5041 cagcgccccg gaggtcgcca tccccgccgc caggttgtgg gtgttcggca cgatgcctgt
     5101 agttccccag aggccgggat gattgagcgg ctcgcttagc acctcgatca tgaacatcgt
     5161 cgtgccgtac atgagcatga ggtcccccgg cttctgcacc ccgacgctcg cgccctcact
     5221 ccaggcgtct atggtcccgg ccgcaaccgg cgtcccggtc ggtatcccgg ttgcctcgga
     5281 cgcctgctgc gttacctccc cgaccacctc ggccggccac aggaggcgcg gcagcggcaa
     5341 gccgggcgcg atttcctcgg cccactcgtc gatccagtag taggagtgca cgtcgtagag
     5401 cgggtcgcac tgactcgccg agtggtggtc caggacgtac tcgccggtga gccgccacgc
     5461 gatgaacgag ttcgccatga agaacttacg ggctttctcc cagacttccg gttcgttgcg
     5521 ccggagccag aggagcttcg ggccgatggc ttgggtggtg agcacggagc cgcagcgccc
     5581 gaggagggtc tccgtaccgt agcgctcgtt gagctcctcg atctcccgcg tcgcccgggt
     5641 atctatgccg tagaggatcg caggccttaa gggtcgtccc tcctcgtcgg tcggcaaaag
     5701 acacgctcca atgccgctcg tgcagaccgc agctatacga tcgtcggcct cctccaagag
     5761 ctcccggcag atggagacga agtctgccca ccacacccct tcggcgtcgt gctcggccca
     5821 accggggtgg ggtagcgaga gctcatgcgg cctctcggca gtggccacga cctcaccgtc
     5881 gggcctagac agtacaccct tagagctgga agttcctatg tcgatcccta gtagaagttc
     5941 gctcaaaata tcattccagt actacctcgg ctgcgtagtg gtcgttgctt gagcccaggt
     6001 tgagcggcca cctggcattg ccgccggtgc accttccgga aaatgcaccg gcgacggatg
     6061 gttagggtca aaagtcgtac tggtcgatgt tctccttcga gaatacgatt ggcgggccga
     6121 gcaggacctc gtcttcggcg agcaacttga cctcgccgag cctaccggcc ttgaaggtgc
     6181 ccgacttggg caggttgccc tcgacctgag cgttagccac gtacaccgcc aggtacccga
     6241 gatctacggg gttccagagg acgaactcct tgacggcgcc ggactcgatg tacggcttca
     6301 tctcgttcgg ggtggagagg cccgtcaccg gcacttctcc cgcgcggccg atctgctgga
     6361 cggcctccgc cgcccctggc agggccacgg aggttatgcc aaagacgccg gctgtattgg
     6421 ggttggcccg gaggtagttc ttggttatgt cgatgccgag ctggaggtcc tcctcgccag
     6481 gctcgacgct agcgatagac atctcggggt acttttcctt catgcgttcg cgcatctctt
     6541 tgagccaagc gacctggttg ggtgcggtga gcgagcccgt aacgaccagg aactcgccct
     6601 tgccaccggt ttgctcggcc atgacatcta ccagtttgta acctatggct tggaaggtgg
     6661 cctggttgac gaagacgtcg cgggcgtcgg gtgccacgtc ggcgtcccag gccccggtgg
     6721 cgatgccagc gttgtcggcc ttcttcatcg cgggggcgag ggcgttcggg tcgttggcag
     6781 ccaccgtgat ggcgtcgcac tgctgctgga tgaactgctc gatcatctgc gactggagcg
     6841 ccgctttggc ctcggtgggc ccgtcgtaca cgagatcgac atccaattcc ttggccgcct
     6901 catttgcccc ctgctcggag gcgttgaagt aagggatgcc gaccagcttg ggcatcatgc
     6961 agatcttgtt gggtcctccc ccacctcccc ctccttgaga cggaccctgc tggcccaccc
     7021 cgcaagcggc gacggccaga accaggaccg ccaacaaggt ggcccgcaac actttaggta
     7081 accacgcacg acgtaacata ctgtcgcctc cttcccagag tttcgctttt cttttacgaa
     7141 cgtctctcat cacccaggcc tatcctactc atcccctttc cggaagaact cattgataaa
     7201 cacggccgcc aagagcagga cgcccagcac gaagagctgc caggtgctgg gcaccccggc
     7261 cagaatcagg ccgttgcgca gcgtagcgat gatcaggacg ccgagcacgg tgccgccgat
     7321 cgtccctgac ccgccgtaga tgctcgcgcc tcccaagacc acggcggaga tcacgtcgag
     7381 ctccagcccg acaccggagt cgcccctggc ggtgtagacg cgggaggtgt agatcaccgc
     7441 cgccatgccc accagaaggc cgatacccga gtacagcact agtttgacct ttcggatggg
     7501 cacgcccgag aagcgggccg cctcctcgtt gttgccgatg gcgtagacgt agcggccgaa
     7561 gctggtgcgg gaaaggatca cccacaccac cgccacggcc atgacgaaga taaagagctg
     7621 acccgggatc ggtcccaggt aggcctggcc gaagtaggcg aaccagccgg ggaagtcgga
     7681 gaccgcctgg gccttcgaga tgccgtaggc caggccctga aagagggcca gggtgcctag
     7741 ggtgacgacc agcgggtgca ggttgaggag ggtgatgaag aggccgttga agagcccggc
     7801 ggccacgccc accacgaggc cgaacaggat ggccagaggc aggggcaaac ccgcggcgaa
     7861 ggagaacccg acggccacgg aggcgagggc gaggttggac cccaccgaga gatctatgcc
     7921 gccggtgatg atgatgaggg tcatcccgag ggccatgagc ccgacctcga cgaagaagcg
     7981 cgaggtgttg agcatggtgg tcggggtcaa gaacaaaggg tccaacaggg tcatcacgac
     8041 taccagcgcg atcagcaccc cgagcaggac aagctcccgg gagaacttca gacggcgcat
     8101 ggttccgacg agcgcgctca tgccttatcc cccacgagtc gccggcgcag cacgtcggtg
     8161 gcgacggcga gcaggatgag cccccccagc acagcgtcct gccatatgcc gggcaccccc
     8221 atcaggatga ggccgttgag gatcacgcct atgagcacag cccccgtgag cgcggccaga
     8281 gacgaacccc gcccccccga gatgctggtc ccgcctatca ccacggcggc tactgcctgc
     8341 agctccagcc cgacccccgt gttggtctgc acggacccgg ccctccccag gtagatcatg
     8401 gacgcgatgc cgacgaacac cccgaccagc gtgaaggcca cgaagcgaac gcggtttacg
     8461 ggcaacccag cgaagcgggc agccacacgg ttgccgccga cggcgaaaac gttgcggccc
     8521 caggtcgagt gacgattgac cagctccata accagaaaca agccgaagag gatcaatacc
     8581 gggacgctga tacccagcac cttccccgta cccagaaagc gggtcggttc ggggacaccg
     8641 gtgatccagg taccccctgt gacgagcagg atgacgcccc gcagcgcgta gagcatgccc
     8701 agcgtcacga tgattgccgg gatgcgtccc agcgtgacca gggcgccgtt gatcgcacct
     8761 gccagagcac ccacggcgac gccggcaagg agcggcagaa ggatcccccc ggtggagagc
     8821 gagactgcgc ccgctgctat cgcgcagaga ccgagcatag aacccgtcga gatgtctatc
     8881 tcggcgcaca gtatgacctg gttgacggcg agggcgatga cacccagcac ggccaccgag
     8941 tccacgatcg actcgaggtt gtaggccgtc agaaacctgt cgttagcgag cgtgaaagct
     9001 accagaagaa cggccaggaa cgccagggcg ggcgccgccg ggcgtgcgag gaaccttcga
     9061 accgactcgc cgcccgcgcc gccacgttct gtgggcgtcg gcgcccgtgt ctcactcatc
     9121 gccggactcc tctcccttgt cgttggatcc gtcgcctccc ccggtggcgg cggtcatgac
     9181 cctctcctgc gtggctcctt ctcgctcgaa ctctccggtg atgcggcctt cgcgcatgac
     9241 caggatccgg tcggacatgg agaggacctc ctccaactcg gaagatatga gcagtatggc
     9301 cttgccctcc ctggccaggt cgtctatgag gcggtagatc tccgatttgg cacccacatc
     9361 tacaccgcgg gtcggctcgt cgagtagcag gacagacggc tcgcgggcca gcgacttggc
     9421 cacgaccacc ttctgctggt tgccgcccga gagccgggcg acggggtcgg acatctcggc
     9481 gccccggatg gagagctcct tggcgaaccg gtcggctgtc tgtctctccg accagggtac
     9541 gaacccgaac cgcgagatgc ggtccaggat gctcagggtt atgttgttct tgatggagaa
     9601 gggcaggatc aagccctgag agcgcctctc ctccggcacg tagaatatgc cccgatccat
     9661 ggccgcctgg ggcgagcgga tcctggcctc ttccccatcc acgagtattt cgccgaactc
     9721 cggcggacgt atgccgaaga tcgcctgggc aagctcggtc cgtcctgctc cgacgagccc
     9781 ggcgaggccc agaatctcac cccggcgcaa cgtcaagtcc acttccgcga actcaccctc
     9841 ccgggtcagc ccccgcacct cgagggcaac ctcgccgggg tcgtgaggat gccggctgaa
     9901 cagctcgtgc aggggacggc ccaccatgag gcggaccagc tcctcggcgt ccagttcgga
     9961 ggcgggcgcg gtggtgatga gtcggccgtc ccgcagcacg gtgatgcggt cggctatctc
    10021 gaagatctcc tccagcctat gcgagatgta gacgacgccc acgtcgccgg aagtgagctc
    10081 ctctatgatg cggaagagtt gccgcgcttc ccggtcggtc agagacgagg tcggctcgtc
    10141 catcaccacc gcccgagcgt cgcctatcag ggcgcgggcg atagccacga tctgctggtt
    10201 ggccgccgaa aggcgcttga cccgggcggt aacgtcgagg tctacaccca gtgagcccaa
    10261 ccagcggcga gcttcggccc gcatggcgtt ccggtccaaa ccgccccagc gggtgcgagg
    10321 tctcttgcgg cccacgaaca ggttctcggc cacggagagc tcggggaaca ggtcgagctc
    10381 ctgggggatc atggcgatgc cggccgcctc ggcgtcgcgc ggcgaagaga actcggccgg
    10441 ctggccgcct acctccaatc ttccctcatc gggtgcgtag agtccatcca cgatcttcat
    10501 gagcgtgctc ttgccggcgc cgttctcgcc cactagggcg tggacttctc cgggccgcag
    10561 gtcgaagtcc acatcgcgca cggcgaccac gccgccgaac cgcttcgtga cacccgcgag
    10621 ctggaagagg ggtggagatc catcacccgt cgcggtgcca ctccctatgt tcgtaggctg
    10681 ctccataccg cgaccgatct cctttccttc ctcgctcact taagtcaccg cgccttcgag
    10741 cggggcgtcc atcaactccg agaaccttcc gaacgcttca tcccactcgt ccgctttatc
    10801 ctcgggggcg taggtctgga cctcggcgga agattgcacg atgtcccgga tctcctgcag
    10861 tgaaccgacg tagccgcggg cgtacgcctg ggccatgacg ttgcccaggg ccgtggcctc
    10921 gaccggcccg gccacgaccg gacgacgggt ggcatcggcg gtgaggcggc acagcatggt
    10981 attctgcgcc ccgccgccaa cgatgtggat cgtctcagca ggctgtccgc tcacctcctc
    11041 ggctcgctcc aggacccacc ggtacttgag cgcgaggctc tcgaacacgc accgcgcgac
    11101 cgcccccggc tcctccccga ggttctgtcc cgtcttcgtg cagaagtcgc ggacgcgcgc
    11161 gggcatgtcc ccgggagcga ggaagccgga atgatccgga tcgaccaagg caccgaactg
    11221 gggcgcggtc tcggccaagc gaaccaattc ctcataggag taatcacggc cgttccggac
    11281 ccaggtcctc cggcactcct gcagcaacca gagccccatc acgttcctca gcacgcgcac
    11341 cgtcccgccg aatccgccct cgttggtgaa gttgtggtgc agcgcaccct ccgtggtgac
    11401 agggtggggc atctcgaccc ccaccagcga ccacgtgccg ctggagatgt acgcgaaggg
    11461 cccctcggcg ggcactgcga cgacggcaga ggcggtgtcg tgcgtcgccg ccgccgttac
    11521 tgggacttct cccaaccccg tcccttcgcg tacctccggc aacaaggttc cgagctgcgt
    11581 gccgggctgg atgatctccg ggaagatgcg ctcgggtacg ttcatccttc gcatgagctc
    11641 ccaagcccag tctccagcct cgaggtcata gagctgggtt gtggtggcaa cggtgtactc
    11701 gcaggccttc tctccggtga gccagtagcc gatgaggtcg ggcatcatca ggagcgtgta
    11761 ggcggcttct agcaacagcg agtcctcgaa agccaggagc tggtagaggg tgttgagcgg
    11821 catgaactgg atcccggtcg tgcggtagat ctcctcctca gggacacgct ccgcagccct
    11881 ctccatcatt ccctcggttc gggggtcgcg gtagtggtac gggttcgaga cgagggctcc
    11941 gtcccgatcc aggagtgcga agtccacacc ccaggtgtcc acccctacgc tcgccgggtt
    12001 cccgctctcc cgggcggctc tggccaggcc ctccttcacc tcgtgaacga tgcgcaacac
    12061 gtcccaatgc agaccgtccg ccacccgcac gggcacgttc gggaaccggt ggatctcctg
    12121 caaatggacc cgttctccgt cgaaactccc taggaagacg cgaccgctct cggctccgag
    12181 atccacagcc aggaagtcag ccctcgcgct cacggtctca ccgggtgcgc tatggtggtc
    12241 atgagcgaga gggcggcttg agtagtgtcc atccgggagc actggatcac gacggggatg
    12301 ctcgattcca ctcggatcgc gtagggtacg ccgcgcggaa tctcggccct gcctaactgg
    12361 tcggttctta tgtgccgggt gcgctccggc gggacggtcg ccatcacatc cttgagcggc
    12421 tcccggtcct cgaagtagaa ggagatcgag agccgcgcct tctccgccga agtattcagt
    12481 acacagatgg cctcatggct ctcctgcgat cccgaactca cttccgggaa gaacccgtcg
    12541 gggatgagcc agacgtaagc accctcgctc attgccttct cctaaacacg ccgtacgtcc
    12601 acctctgcga tggtgagaga ctccaagcgc tcctcgggag cgtcgaccac gagcgcccgg
    12661 gagagcttcg aggcgtgcgc tatgacgctt aagcccctcc tctcaaactt gctgcggtcc
    12721 acgagaagga tgggctcctc ggagtgctcg atcatggcgt gcttgacctc ggcctctaga
    12781 acgtcgggat ctgtcaggaa gccctcgcgg gtaacgccct tgacgctcac gaaggccctg
    12841 tccgtggagt gcgccgagat agttgccacc gcgtgcgggc ccacgaagga gagcgtgagc
    12901 ttcctcatga acccgcctac cccgacgacg ccggtcttgg gcgcctcgtt cttcatgaac
    12961 agctccatca ctggcacgga attggtcagg agcgtgacct gcggtccctc cctcagtatt
    13021 ctgcgcgcca ggtagtacgt cgtggtggaa caatccagga agacagaatc gcccggctcc
    13081 agaagcccga cggccgcctc ggccaggcgc tccttggcct ccaccgcttt gtccaaccgg
    13141 taccagaacg agtcctcgtg tcccgcatat tctggaagta ccgctccccc atgcgtcctc
    13201 cgcacccggc cctcctcctc gaggacgatc aggtctcgcc gggccgtcat aggcgagatc
    13261 ccgaattcgg cctccaacag agcgatcgtg acgcctccgg tctcccgaac gagctgcgct
    13321 atccgccgac gtcgcttctc cggtatcaat gcctgtctct ctggtatttt gtggtcgttt
    13381 tgttctattt tcccacctaa tcacccagtt cttatcatta gtagcagaag caacgctctc
    13441 gcgcaagaga gcgccaactt gctaattacg tgtgacaaat agggtgttgc tgggacttgc
    13501 ccaggtttgc gaatccccca tatttaagcc gggttctgtt gcctgcctta ccctgtgttg
    13561 caccgtattg cgct
//
LOCUS       DATMJA010000023         4314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_596019, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000023 DATMJA010000000
VERSION     DATMJA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596019"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VNT12_00575"
     CDS             complement(<1..429)
                     /locus_tag="VNT12_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWI44630.1"
                     /translation="MTEIASRGARDFDEEYREDPLWYKDAIIYQLHVKAFFDSDNDGV
                     GDFRGLTQRLDYIKDLGVTMIWLMPFYPSPMRDDGYDISEFKNVHHDYGTRRDFRAFV
                     RKAHELGLKVITELVINHTSDEHAWFQRARKAPKDSAKRNF"
     gene            complement(426..2372)
                     /locus_tag="VNT12_00580"
     CDS             complement(426..2372)
                     /locus_tag="VNT12_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HWI44631.1"
                     /translation="MRVAFEPVIIENVYPELDCGRYPVKREVGDSFEVWADLFKEGHD
                     VLAAALKYREKGASGWSETPMRFHENDRWRGGFTLTKNTRYEYTIEAWMDDFETWRAE
                     IGEKVDAGQNVELELIEGCSIVEGAYSRSQDKRLKEMLSSIDAAGYEGRFALLRSNEL
                     RGLMREHPDRSTSTVYGKTLEIVADRERARYGAWYEMFPRSQGTEPGRSATFGEAERR
                     LPEIGGLGFDVVYLTPIHPIGLSHRKGRNNVLNAGPDDPGSPYAIGSEEGGHKAVHTG
                     LGTLEDFRHFVEACHEHGMEVALDLAIQCSPDHPYIKEHPEWFNFRPDGTIKYAENPP
                     KKYQDIVNVDFYSEDRENLWQEWLGVILFWVEQGVRIFRVDNPHTKPFPFWEWAIGEV
                     QEEHPGVIFLSEAFTRPKVMKALAKLGFTQSYTYFTWCNHKQEIMEYLTELTQDWPKE
                     YMRPNFFPNTHDILPHILQFGGRPAFQMRFVLAATLSSTYGIYSGFELCENTPRGEPG
                     TTLYYQDSEVYEYKVWDWDRPGNIKEYIKRINEIRHQNPALQYLKNLSFYASPDDNIL
                     FYGKRTGDNAVFVAVNLDPHEAHDTHVQFPLWELGISEDEGYVVEELMTEREIPTTGS
                     WFWVRLDPRSNPAEIFRLRKVVER"
     gene            2645..2914
                     /locus_tag="VNT12_00585"
     CDS             2645..2914
                     /locus_tag="VNT12_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI44632.1"
                     /translation="MAAQRAGERTVFGRRIGENQGIQFPKARAYANVEAASLMVEKAA
                     SLFDRGEPCGAEANLAKLLAADASWEAANAALQRLAATASTPSTT"
     gene            complement(3256..3786)
                     /locus_tag="VNT12_00590"
     CDS             complement(3256..3786)
                     /locus_tag="VNT12_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HWI44633.1"
                     /translation="MRRIFGAVTVECVMGDITSQDDVEAVVNAANARLEPGGGVAGAI
                     HRASGPGLAEEAVPLGPIQPGEAVITGAHGLPNRYVVHTLGPVYGQDRPEAELLGGCY
                     RNSLALAEEYSVESIAFPAISTGVFGYPVEEAAGVALGTIKEEADGLEHVRLVRFVLH
                     SASDLQAHERVLSELE"
     gene            complement(3783..>4314)
                     /locus_tag="VNT12_00595"
     CDS             complement(3783..>4314)
                     /locus_tag="VNT12_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI44634.1"
                     /translation="VAGVTHLFNGMSAMHHREPGLPGAAFAHPRAVCGLIADGLHVHP
                     EMVGLAFRMLGPDRLCLVTDAIAATGMEDGEYRLATRTVYTDGGVPRLGSGSLAGSMF
                     TMREAFMNILAFTGCTVPEAARMTSTSSAKLVGEGRRKGRLVPGYDADVTVLTPDLSV
                     ETVWRGGKLAYSWEGG"
BASE COUNT          752 a         1374 c         1313 g          875 t
ORIGIN      
        1 gaagttgcgc ttcgcagagt ccttcggtgc cttccgggcg cgctggaacc acgcgtgctc
       61 atcggaggtg tggtttatga ccagctcggt tatgaccttc agtccaagct cgtgcgcctt
      121 gcgcacgaag gccctgaagt cccgcctggt gccatagtcg tggtggacgt tcttgaactc
      181 ggagatgtcg tagccgtcgt cgcgcatcgg cgagggatag aacggcatca gccagatcat
      241 cgtgacgcct aagtccttta tgtagtcgag gcgctgcgtg aggcccctga agtcccccac
      301 gccgtcgttg tccgagtcga agaacgcctt gacgtgcagc tggtagatga tggcgtcctt
      361 gtaccagagc ggatcctcgc ggtactcctc atcgaaatcc ctcgctcccc tagatgcgat
      421 ctccgtcacc tctcaaccac cttcctgagg cggaagatct ccgccgggtt gctcctcggg
      481 tcgagcctga cccagaacca gctccccgta gtcggaattt ccctttccgt catgagctcc
      541 tccacgacgt aaccctcgtc ctccgagatc ccaagctccc acagcgggaa ctgcacgtgc
      601 gtgtcgtggg cctcgtgggg gtctaggttc accgcgacga agacggcgtt gtccccggtc
      661 cgtttgccgt agaagagtat gttgtcgtcc ggggacgcat agaacgagag gttcttcaga
      721 tactggagcg cggggttctg gtgccggatc tcgtttatcc gctttatgta ctccttgatg
      781 tttcccggac ggtcccagtc ccagaccttg tactcgtaca cctcggaatc ctggtaatac
      841 aaggtggtac ccggctcgcc ccgcggggtg ttctcgcaca gctcgaagcc gctgtatata
      901 ccgtaggtgc tcgacaatgt ggccgcgagc acgaaccgca tctggaatgc gggacggcca
      961 ccgaactgga ggatatgcgg caggatgtcg tgggtgttgg ggaagaagtt gggtcgcatg
     1021 tactccttcg gccagtcctg cgtcagttcc gtcaggtact ccatgatctc ctgcttgtgg
     1081 ttgcaccacg taaagtacgt gtaggactgg gtgaagccaa gtttggccag ggccttcatc
     1141 accttcggtc gggtgaaggc ttcagagagg aagatcaccc cagggtgctc ctcctgtacc
     1201 tccccgatcg cccactccca gaaggggaaa ggtttggtat ggggattgtc cacccggaag
     1261 atcctgactc cctgctcgac ccagaacagg atcacaccga gccactcctg ccacagattc
     1321 tccctgtcct ccgagtagaa gtccacgttg acgatgtcct ggtacttctt aggcgggttc
     1381 tcggcgtact ttatggtccc gtccggcctg aagttgaacc actccggatg ctccttgata
     1441 tacgggtgat caggggagca ctggatggcg aggtcaagcg cgacctccat cccgtgctcg
     1501 tggcatgcct ccacgaagtg cctgaagtcc tcaagcgtcc cgagtccggt atggacggcc
     1561 ttgtgcccgc cctcctcgct accgatggcg tagggactgc cagggtcgtc agggccggca
     1621 ttcaggacgt tgtttcggcc cttgcggtga ctcagaccga tagggtggat gggagtcagg
     1681 tacaccacgt cgaagccaag cccccctatc tcaggcaggc gccgctcggc ctccccgaag
     1741 gttgcgctcc ggcccggttc cgtcccctgc gagcggggaa acatctcgta ccacgccccg
     1801 tagcgcgccc gctcgcggtc cgccacgatc tccaacgtct tcccgtaaac ggtggacgtc
     1861 gagcggtcgg ggtgttccct catcaggccg cggagttcgt tggaacgcag caacgcgaac
     1921 ctgccttcgt agcctgctgc gtcgatgctc gagagcatct ctttcaggcg cttgtcttgc
     1981 gagcgtgagt aggcgccctc gacgatactg cagccctcga tcagctccag ttccacgttt
     2041 tgtccggcat cgaccttctc gccgatctcg gcccgccacg tctcgaaatc gtccatccag
     2101 gcctcgatgg tgtactcgta gcgggtgttt ttcgtgagcg taaaaccgcc tctccagcgg
     2161 tcgttctcgt gaaagcgcat gggcgtctca ctccacccgg acgcgccctt ctccctgtac
     2221 ttcaaggccg cggcgagcac gtcgtgccct tccttgaaaa ggtcggccca gacttcgaag
     2281 gagtcgccca cctcccgctt gaccggatag cgaccgcaat ccaactcggg ataaacgttc
     2341 tcgataatta ccggttcgaa cgcgactcgc accccgtctc ctttcccact aatcctcact
     2401 gacctatcct ataggcgccc cactttggat gggagaagat gatctcatat gacggtgcta
     2461 cggacacgcg ggagagttat ggatggatga cggtagagtg cgcgcccggt ccccaagtat
     2521 tacccgggac ggggtacttc gggcgcgtga ccccgcctgt acaccatgac ggttcgcaag
     2581 aaagtgatga ccgtcttgcc ttcctggttg tagcctgtgg ttttcaccgt cacgatgccg
     2641 acgtttggcc gcgcagcgcg ccggcgagcg cacggtgttc gggcgcagga tcggcgagaa
     2701 ccagggcatc cagtttccca aagcgagggc ctacgcgaac gtcgaggccg cctcgctgat
     2761 ggtggagaag gcagcctccc tcttcgaccg tggggagccg tgcggcgccg aggcgaacct
     2821 ggcgaagctc ctggccgccg acgcctcgtg ggaggcggcc aacgccgccc ttcagagatt
     2881 ggcggctacg gcttcgacgc cgagtacgac gtagagcgca agttccgcga gacgcgtctc
     2941 taccaggtcg ccccgatctc gattaacctc atcctctctt acatgtcagg cgcatgaggg
     3001 tatggtctat tgccaagccc ggtagcccac agagtgagat atctttgagt attcccctcc
     3061 taagccgccg ggcgaccgat tgtgtgtaga gcaattcagc acgctccagc cttcggcttc
     3121 cgctcgtcag cacttcagct tgttgttttg gttctctgct gctcgttcgt ggatgagagt
     3181 gcgattcgca gtaggtcaat gcctctgcaa atcgctttgg aagttctatg aagggtaggg
     3241 cgtgcgggaa gaagtctatt cgagttcgga gagcaccctc tcgtgggctt ggaggtcgga
     3301 ggcgctgtgg agaacgaacc tgaccaggcg cacgtgctcc aaaccgtcag cctcctcctt
     3361 tatcgtcccg agcgccaccc ccgccgcctc ctcgaccggg tagccgaaga ccccggtcga
     3421 gatggcgggg aaggcgatgg actccacgct gtactcctcg gcgagcgcga gcgagttgcg
     3481 gtagcagccc cccaggagct cagcctcggg gcggtcctgg ccgtagaccg gccccaaagt
     3541 gtggacgacg taacggttcg gaagcccgtg cgcgccagtt atgacggcct cgccgggctg
     3601 tatggggccg agcggcacag cttcctcggc tagccccggt cctgaggcgc ggtggatggc
     3661 gcctgctacc cctccgccgg gttcgagccg cgcgttcgcc gcgttgacca cggcctccac
     3721 gtcgtcctgg gaggttatgt ctcccatgac gcactcgaca gtcacggccc cgaatatacg
     3781 tctcatccgc cctcccagga gtaggccagc ttgccacctc gccataccgt ctcaacggag
     3841 aggtcgggcg tcaagaccgt cacgtcggcg tcgtagccgg ggacgaggcg gcccttgcgc
     3901 cggccctcgc cgacgagttt cgccgaggag gtagaggtca tacgcgcagc ctcggggacg
     3961 gtgcagccgg tgaatgccag gatgttcata aacgcctctc tcattgtaaa catgctccct
     4021 gcgagggacc cgctccctag cctcgggacg ccgccgtcgg tgtacacggt gcgggtagcc
     4081 agacggtact caccgtcctc catacccgtc gcggctatgg cgtcggtgac gaggcagagg
     4141 cgatcaggac cgagcatgcg gaaagcgagc ccaaccatct cggggtggac gtgcagaccg
     4201 tcagcgatca gaccgcagac ggccctcgga tgggcgaagg cggccccagg cagcccgggc
     4261 tcgcggtggt gcatcgcgct catcccgttg aagaggtgcg taacgccggc aacc
//
LOCUS       DATMJA010000024         3680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_63184, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000024 DATMJA010000000
VERSION     DATMJA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63184"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VNT12_00600"
     CDS             complement(<1..603)
                     /locus_tag="VNT12_00600"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWI44635.1"
                     /translation="MGADLHAAHSNALVSGAATDSRKVREGDLFFALRGRTDGADFAP
                     EAHLRGAVATVATRRLDEPTLVVDDPLEALQRLARWTLLRQDTPAVVGITGTVGKTTT
                     KDALATILRHAGKRVSATAGNLNNEIGLPLTILASDPRTEVLVLEMGATHAGDIQHLC
                     AIAPPDIGVLTAISPVHLDSFGTLEGLAAAKGELALSLPES"
     gene            complement(636..2237)
                     /locus_tag="VNT12_00605"
     CDS             complement(636..2237)
                     /locus_tag="VNT12_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWI44636.1"
                     /translation="MVAAVFAVTGLLLGGRAVHISLTEDENYRAFADEQAGGTTPVAA
                     PTRGSIMSADGRELATSLEVASVIATPYQIEDARATARKLHAVLSKETDTSEKEIRVS
                     LLRRGADGQLSGYSVVAAGVEPETAAKVQALGIEGITTAPDTMRVYPDGSLASQLLGH
                     QGDYGEPFGGVEASYDDTLKRGSDVDLTVDSAVQQELQKALAKAVEKSKAKSAVGVVM
                     RVDDGSILALANTPAYDNNEFGDVPAEDQRDRVLTDPYEPGSTFKAFTVASALEDGAV
                     TPEDKFVVPDHMTVADHVINDSQPHETEILTPAGVLEHSSNVGATKIAQELGGKKLYG
                     YMRDFGFGKPTGVDLWGEDLGIVPAYKEWSGISIGNIPFGQGFTVTPLQLVSGYATLV
                     NGGRRVTPHVAEQETSPKQGPRVISKKTSAIVRGMLQGVVDDGSGHFAQISGYTVAGK
                     TGTSQIVDPKTGTYGDQYVASFIGFAPATDPEYVMLVAVDDPQTSYWGELVAVPAFRH
                     VMSFTLGYFNVPPDRRGFEAEEPLW"
     gene            complement(2449..2886)
                     /locus_tag="VNT12_00610"
     CDS             complement(2449..2886)
                     /locus_tag="VNT12_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44637.1"
                     /translation="MAASQRRYKATLPTEPAWPAQKRAQERPQDRTLRRKAAGRRRRF
                     AMLVVVPVLLMLGSVYLNTLAAGLDARAAELGVRLDRAREVGERLEVKVAELSGAERI
                     RALAAEKLGMHEPKSTTLKVYGARAEDGKNDGASQEGGRPRQR"
     gene            complement(2886..>3680)
                     /gene="rsmH"
                     /locus_tag="VNT12_00615"
     CDS             complement(2886..>3680)
                     /gene="rsmH"
                     /locus_tag="VNT12_00615"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWI44638.1"
                     /translation="SLIGIDRDPEAAGRASDLLQDPRFEFVSGAYDLALWAMVEERRS
                     VDAILFDLGLSSFQVDDTGRGFSYMGEGPLDMRMDPRSGTTAADFLNTVEVGELSRVL
                     SEYGDVPRGEARRVAREIVRRRPLAVTTDLYEAVRAAVGWKERGGNPAKRIFQAVRVR
                     VNDELGSLSRALEASERLLTPGGRLVVITFHSGEDRLVKRFISGREGRCVCPPELPVC
                     VCGAKPVFRRGEVLTPTRREIEENPRSASARLRAAIRTAEPPVDAS"
BASE COUNT          582 a         1245 c         1125 g          728 t
ORIGIN      
        1 agactcaggt agggagagag ctagttcgcc tttcgcggct gcgaggcctt cgagcgttcc
       61 gaaggagtcc agatggaccg gcgagatggc tgtcagaaca ccgatgtcgg gcggggcgat
      121 ggcgcacaga tgctgtatgt cccctgcgtg ggtggctccc atctcgagga cgaggacttc
      181 tgtgcgaggg tcggaggcca ggatggtgag gggaaggccg atctcgttgt tcaggtttcc
      241 tgccgtcgct gagacacgct tcccggcgtg cctgagaatg gtcgcgaggg cgtccttggt
      301 cgttgtcttg cccacagtgc ccgtgatgcc gacaacggca ggcgtgtcct ggcgcagaag
      361 cgtccaacgc gcaagcctct gcagcgcctc cagagggtcg tccacgacga gggtcggttc
      421 gtccagccgg cgggttgcca ccgtcgccac cgcgccgcgt aggtgcgcct caggggcgaa
      481 gtccgcgccg tcggtcctac cgcgcagggc gaagaacagg tccccttcgc gcaccttacg
      541 agaatcggtc gcagccccgc tgacgagggc gtttgaatgg gcggcatgga gatccgcgcc
      601 cattgcctgc gcagcctcta aaagagtagc cggcctcacc agaggggctc ctcggcctcg
      661 aaacccctac ggtcaggcgg gacgttgaag tagcccagcg taaagctcat tacgtggcgg
      721 aaggccggta ccgcgaccag ctctccccag tatgaggtct gcggatcgtc tacggcaacc
      781 agcatcacgt actcaggatc ggtggcaggg gcgaacccaa tgaaagaggc cacgtactgg
      841 tcgccgtagg ttcctgtttt cgggtccact atttgagagg tccctgtctt accggccacg
      901 gtgtaaccgg agatctgggc gaagtggccc gacccgtcgt cgactacgcc ctgcaacatt
      961 ccgcgtacta tagccgaggt cttcttgctt atcaccctgg gaccctgttt cggggaggtc
     1021 tcctgttctg ctacgtgtgg tgttaccctt cgcccaccgt tgacgagggt ggcgtagccg
     1081 gagactagct gcagaggcgt aaccgtaaag ccctgtccga agggtatatt tcctatcgag
     1141 atcccgctcc attccttgta ggcagggacg atccccaagt cctcacccca caggtctacg
     1201 cctgttggct tgccgaagcc gaaatcgcgc atgtagccgt aaagtttctt cccgccaagc
     1261 tcttgggcga tcttcgtcgc ccctacgttg ctggagtgct cgagcacgcc agctggggtc
     1321 aagatctcgg tctcatgcgg ctgggaatcg ttgatgacgt ggtccgctac tgtcatgtga
     1381 tccggaacca cgaacttatc ctcaggcgtg accgcgccgt cctcgagggc ggaagcgacg
     1441 gtgaaagcct tgaaggtcga gccgggctcg taaggatcgg taaggacccg atctcgctgg
     1501 tcttctgcgg gaacgtctcc gaactcgttg ttgtcgtagg ccggggtgtt ggcgagcgcc
     1561 aagatcgacc cgtcgtcgac gcgcataacc acgccgacgg cgctcttggc cttgctcttc
     1621 tcgacagctt tcgccagggc tttttgcaac tcctgctgca ccgcggagtc aaccgtcaaa
     1681 tctacatcgc tgccacgctt caaggtgtcg tcgtaactgg cctcgacccc gccgaagggc
     1741 tcgccgtagt ccccctggtg tccgaggagc tggctggcga gggacccgtc cgggtagacc
     1801 ctcatggtat cgggggcggt tgtgatgccc tcgataccca gggcctgcac cttagccgct
     1861 gtctccggct cgacacccgc ggcgacgacg ctgtatccac tgagctggcc gtccgcacca
     1921 cgcctaagga gagagaccct tatctccttc tcgctcgtgt ccgtctcttt gctgagcacg
     1981 gcgtgcagct ttctggccgt cgcccttgcg tcttcgatct ggtaaggggt tgctatcaca
     2041 ctggcgacct cgaggctggt cgcgagctcc cttccgtcgg cactcattat gctgccgcgg
     2101 gtcggcgcgg cgaccggggt cgttccaccg gcctgctcgt cggcgaaggc cctgtaattc
     2161 tcgtcctccg tgaggcttat gtgtacggcc ctcccgccga ggagaagtcc cgtcaccgcg
     2221 aacaccgccg ccaccatcca cagcctcctg tggcccacgg gagtcctggc ttcggggccg
     2281 atcgatgacg tgcgacgtgc cctggtggcg ccacgcttcg cgccgccggc ccgcctctgg
     2341 ccgcggagtg gtatgacttt gccaccagag gacttgcgcc ctttgtttct ggtgcgaggg
     2401 ggggttttct tgccacggcg aatccttgcg ttgcgaggcc ctggccggtc aacgctgccg
     2461 cggtcttccc ccttcctgtg atgccccatc gttcttccca tcctccgctc ttgctccata
     2521 gactttcaag gtagtgctct tcggctcgtg cataccaagc ttctccgccg cgagggcccg
     2581 gatcctctct gcgcccgaca gttcggcgac tttgacctcg agtctctccc ctacttctct
     2641 ggccctatca agcctgacgc cgagttcggc ggccctggcg tcgagccccg cggcgagcgt
     2701 gttcagatag acgctcccca gcatcagcag cactggcacc acgaccagca tcgcaaaccg
     2761 gcgacgccgg cccgcggcct tccgcctcag ggtgcgatcc tgcgggcgct cctgggcgcg
     2821 cttctgtgcg ggccacgccg gttctgtagg gagcgtagcc ttgtacctgc gctgggaggc
     2881 ggccatcagc tggcatccac cggaggctcg gccgtcctta tcgccgcccg cagcctggcc
     2941 gaggcgctgc gcgggttctc ctctatctca cgccgggtag gggtcagaac ctcgcccctc
     3001 cggaatacgg gctttgcgcc acaaacgcac acaggcagct ctggcggaca gacacaccgc
     3061 ccctcccgcc ctgagatgaa tcgctttact aggcgatcct cccccgagtg gaaagtgatg
     3121 accacaagcc tcccacctgg tgtgagtagc cgttctgagg cctccagagc ccggctgagg
     3181 ctgcccaact cgtcgtttac tctgacccgc acggcctgga agatcctctt cgccgggttg
     3241 ccgcccctct ccttccagcc cacggccgcc cgtacggcct cgtacaggtc cgtcgtcacc
     3301 gcgagcggtc gcctccgaac gatctcccgc gccacacgcc ttgcctcacc gcgcggcacg
     3361 tcgccgtact ccgagagcac cctcgacaac tccccgacct cgacggtgtt caggaagtcc
     3421 gcggccgtcg tacccgaccg tgggtccata cgcatgtcca gtggtccctc gcccatgtat
     3481 gagaacccac gtcctgtgtc gtcgacctgg aaactggaga ggccgaggtc aaacaggatc
     3541 gcatcgacac ttcggcgttc ctcgaccatc gcccacaacg ccaggtcgta ggcgcccgag
     3601 acgaactcga agcgcgggtc ctgcagcaga tcagaggctc gccctgccgc ctcggggtcg
     3661 cggtcgatgc caatgaggct
//
LOCUS       DATMJA010000025         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_715070, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000025 DATMJA010000000
VERSION     DATMJA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715070"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /gene="rapZ"
                     /locus_tag="VNT12_00620"
     CDS             complement(<1..857)
                     /gene="rapZ"
                     /locus_tag="VNT12_00620"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWI44639.1"
                     /translation="MHPRRDGRRVVDEQAAPDTDSRVSGKRRIVIITGLSGAGKSNAL
                     QAFEDAGYFCIDNLPPRMIPAVMETTAQGNGGPDGVVVVVDMRGRRYFGEELERNLEA
                     VEKEKGWEHRILFIDSDDPTLVRRYKESRRPHPAARDGDVLEAIRRERRDLANLREMA
                     DVVVDTSGYSALDTRLRFKKLAESFASRMTVSLISFGFKHGTPLDVDTLLDVRFLPNP
                     HYDPDLRPLTGQDAPVREAVLGSEDCREFLEKTRALLSFLIPRYAAEGKTYFTVGVGC
                     TGGRHRSVA"
     gene            complement(938..2776)
                     /gene="uvrC"
                     /locus_tag="VNT12_00625"
     CDS             complement(938..2776)
                     /gene="uvrC"
                     /locus_tag="VNT12_00625"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWI44640.1"
                     /translation="MTENGFPDRSHLPTSPGVYIFKDAGDKVIYVGKAKNVRSRVASY
                     FNRSGDGRPKIAELRERVRQIDFIVTGSEIEALVLEANLIKRHRPRFNASLKDDKSYP
                     YIVITTGDDYPRVFATRSSHDPRHRYFGPFPSAGSVHATLDVLNKTFPFRKCRGPEPG
                     RRSGVPCLNYHIGRSAAPCIGAVSKGDYDGIIADVISFLEGRVDGLIREREAAMREAA
                     RDMDFERAAKLRDEISALSHVRDRQQATIASEDSFDAFGAYTEGETACVQVFAVREGQ
                     IVNRDSFLLDNYGESRAEDVALQFVPQYYGTAAVPREVLIQSDDREDELHTLADHLSG
                     LRGTSVEVRRPRRGDKRRILEMALRNAELGLEHERMLDEARRNRVASTLDGLREELAL
                     PRLPVRIECYDISNTMGTNSVASMVVFQGGRPAKSEYRRFRVRTVEGADDFASMAEVI
                     RRRLERLQADDEKFTPTPDLILLDGGKGQLSAVVPVLEEMKVGGELPDIPLRSLAKRE
                     EEIFEPGRPEPVILERNSPELHLLQRVRDEAHRFANAYHTKLRGKAMTASVLDDLPGV
                     GPERKKKILAHFGTPEAFLNATLEELESVPGLPGRVAREVHAKMHR"
BASE COUNT          478 a          851 c          930 g          548 t
ORIGIN      
        1 atggctacgg atcggtgcct gccaccggtg cacccgacgc cgacggtgaa gtaggtcttg
       61 ccttctgcgg cgtagcgggg tatgaggaag gagagcagtg ccctagtctt ctcgaggaat
      121 tcccggcagt cttctgagcc gagaaccgcc tcgcgcaccg gcgcgtcttg gcctgtgagc
      181 ggtctgagat caggatcgta gtgggggttg ggaaggaagc ggacgtcgag gagcgtgtct
      241 acgtcgaggg gggtaccatg cttgaagccg aaggagatga ggcttacggt catgcggctc
      301 gcgaaagact ccgcgagctt cttgaaccgc aggcgggtgt cgagcgcgga gtagcccgag
      361 gtgtcgacga ccacatcggc catctccctg aggttcgcga gatcccgcct ctcacggcgt
      421 atggcctcga ggacgtcgcc atcgcgggct gcagggtgcg gcctacggct ctccttgtag
      481 cgcctgacaa gcgtggggtc gtcggagtcg atgaacagga tgcgatgctc ccagcccttc
      541 tccttctcaa cagcctccag gttgcgctcg agttcctcac cgaagtatcg gcgtccgcgc
      601 atgtccacca cgacgaccac cccgtcagga ccgccgttgc cctgcgccgt agtctccatg
      661 acagccggga tcatacgcgg tgggaggttg tctatgcaga agtatccggc gtcctcgaaa
      721 gcctggaggg cgttgctctt gcctgcaccc gaaagccctg taatgatgac gatcctgcgc
      781 ttgcccgaga cccgagagtc ggtgtccggc gccgcctgct catccacgac gcgtcgtccg
      841 tccctcctgg gatgcacaag ccatctcggg aatcgagatt agcaccgcga gtccaagctt
      901 gcaacaggca tcaggattca ggactgatac ctgaaaccta cctgtgcatt tttgcgtgga
      961 cttcgcgggc tactcttcct gggagtcctg ggacggactc gagctcttcg agggtggcgt
     1021 tcaggaatgc ttctggggtg ccgaagtggg ctagtatctt cttcttacgc tcggggccga
     1081 cgccggggag gtcgtcgagg accgaggcgg tcatggcttt gccgcggagc ttggtgtggt
     1141 aggcgttggc gaagcggtgg gcctcgtcac ggacgcgctg gaggaggtgg agttcggggg
     1201 agttgcgctc caggatcacc ggctccggac ggcccggctc gaagatctcc tcctcgcgtt
     1261 tggcgagaga gcgaagcggg atgtcaggta attcgccgcc gactttcatc tcttcgagga
     1321 cggggaccac tgcggagagc tgtcctttgc cgccgtctag caggatcagg tccggagtag
     1381 gcgtgaactt ctcgtcgtcg gcctggaggc gttcgagcct gcggcggatt acctcggcca
     1441 tgctggcgaa gtcgtcggca ccctcgaccg tccttaccct gaaccgcctg tactcgctct
     1501 ttgccgggcg gcctccctgg aacacgacca tggaggccac ggagttggtc cccatggtgt
     1561 tcgagatgtc gtagcactcg atccgaaccg gcagccgtgg gagggcaagc tcctcgcgca
     1621 acccgtcgag cgtggaggcg accctgttgc gcctggcttc atcgagcata cgctcgtgct
     1681 ccagcccgag ctccgcgttg cgcaaggcca tctccagaat tcggcgtttg tccccccgcc
     1741 tgggacggcg tacctcgacg ctagtccccc tcaaacccga gaggtgatcg gcgagcgtat
     1801 gcagctcgtc ctcgcggtcg tcggactgga tgaggacctc gcgcggcacg gcggccgtgc
     1861 cgtagtattg ggggacgaac tggagcgcga cgtcctcggc ccgcgactca ccgtagttat
     1921 cgaggagaaa agagtcgcgg ttcacgatct ggccttcgcg cacggcgaag acctgcacgc
     1981 aggccgtctc gccctcagtg taagcgccga aggcgtcgaa tgagtcctca gaagcgatgg
     2041 tcgcctgctg gcggtcgcgt acgtgggaga gggcagagat ctcgtcgcgc agcttcgccg
     2101 cccgctcgaa gtccatgtcc ctggcggcct cacgcatggc ggcctcgcgc tcgcgaatca
     2161 aaccgtccac ccgcccctcc agaaaggaga tgacatcagc gatgatccca tcgtagtccc
     2221 ccttggagac ggccccgatg cacggcgccg ccgaacgccc gatgtggtag ttgagacaag
     2281 gcaccccgct gcggcgtccg ggctcaggac cgcggcactt gcggaagggg aaagttttgt
     2341 tcagtacgtc gagcgtcgcg tggacgctgc cggcgctagg gaaaggtccg aagtagcggt
     2401 ggcgcgggtc atgtgaggag cgggtggcga agaccctggg gtaatcgtct ccggtcgtga
     2461 tcactatgta agggtagctc ttgtcgtcct tgagcgaggc gttgaagcgc ggcctatggc
     2521 gctttatcag gttcgcctcg aggacgagcg cctcgatctc gctgcccgta acgatgaaat
     2581 ctatctgcct gacgcgttcc ctgagctccg caatcttcgg gcgcccgtct cccgagcggt
     2641 tgaagtagct ggcaaccctg ctgcgcacgt tcttggcctt gcccacgtat atgaccttgt
     2701 ctccagcgtc tttgaagatg tagacaccgg gagaagtagg aaggtgtgac ctatcgggga
     2761 atccgttctc agtcatcgag agggattata ccgcgaggac ccacgct
//
LOCUS       DATMJA010000026         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_122783, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000026 DATMJA010000000
VERSION     DATMJA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122783"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..155
                     /locus_tag="VNT12_00630"
     CDS             15..155
                     /locus_tag="VNT12_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44641.1"
                     /translation="MELSGRGHDRVLRVARTVADLSGEQEIAAEHVAEALNYRRSHLV
                     GR"
     gene            145..1251
                     /gene="dprA"
                     /locus_tag="VNT12_00635"
     CDS             145..1251
                     /gene="dprA"
                     /locus_tag="VNT12_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWI44642.1"
                     /translation="MEDDDRAKYLFLSLLQARVGSSLVAHLGNLAPEAVLRTPVVELA
                     GRAKISEGASRAFEELQRAFDPGRMHNRLAAEGIEALTPVDEAYPGPLKSIPDPPPAV
                     FVNGAVPNTVSVAVVGSRKASATGIETARALGFALSERGVCVVSGLALGVDAAAHEGA
                     VEAGGPTVGVLGCGIDVVYPRRNRGLFQSVRRQGALISEYYLGEAPLAWRFPARNRII
                     AGLAGTVVVVEAPEQSGALITARHALDAGRDVWAVPGPPGVHECRGSNGLLADGAGVL
                     WDLPEFVDLVAREQRVPVADRASGDVQPLPSGLEDTEAAVLSGVGFEPSGVDAVAKRS
                     GVAMPEVLTALALLELKGFVSRNGVGAYVRRAVP"
     gene            1248..2090
                     /locus_tag="VNT12_00640"
     CDS             1248..2090
                     /locus_tag="VNT12_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HWI44643.1"
                     /translation="MSRRTLRSVWSHYLRVRKRLAEAEISGVERRMAEREARGLRDRL
                     VVNYSPLVKYVAGRISGRMSGPLDQEDVVASGLFGLLNAVETYDPERSTKFESYAISR
                     IRWSILDELRKVDPLSRRTRMRAHQIERTRDELVQKLGRAPTESELVGRLGISVTEHR
                     AFIDQYARAQVRSLEARLEYQGGPVAGLHDLIGDHSATDPASAAEMAEVRARLVRAIE
                     GLGEQERVVTTFYYYEGLTLREIGRVLDLTEGRISQILHSALVRLRQNLAEESRLSGR
                     MQGR"
     gene            2280..>2886
                     /gene="rpsB"
                     /locus_tag="VNT12_00645"
     CDS             2280..>2886
                     /gene="rpsB"
                     /locus_tag="VNT12_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWI44644.1"
                     /translation="MQSTTERVGIRELLEAGVHFGHQAKRWNPKMKRYIFAQRAGVHI
                     IDLDQTLTLLERALSFVRGVAEANREVLFVGTKKQAQIPLAEEAIRSGQPFVAERYIG
                     GMLTNFQTILPRIQYFKDLAVRVDETPEEERTGKDWFALNREYQKLRRNFAGITEMER
                     LPGGVFVIDPKREELLVKEANRLKIPVLALTDTNCDPEVIDY"
BASE COUNT          540 a          868 c         1001 g          477 t
ORIGIN      
        1 gggcggtgga ccgcatggag ctctcgggaa ggggtcatga cagggtactc agggtggcta
       61 ggaccgtcgc cgacctctcg ggcgagcagg aaattgccgc cgagcacgtc gccgaggcac
      121 tcaactaccg gaggtcgcac ctagttggaa gatgacgacc gcgcaaagta cctgttcctg
      181 tcgctgttgc aggctagggt agggagcagc ctcgtagcgc acctcggtaa cttggccccc
      241 gaggccgttc tccggacgcc ggtcgtcgag ttggcaggtc gcgcgaagat ctccgaaggg
      301 gcttcgcgcg ctttcgagga gttgcaaagg gccttcgacc ctggccggat gcataaccgc
      361 ctggcggcgg aaggaatcga ggccctcacg cccgtcgacg aggcttaccc ggggccgctg
      421 aagagcatac cagacccgcc gcccgcggtc tttgtgaacg gcgctgtccc gaatacggtc
      481 tccgtcgcgg ttgtcgggtc gcgaaaagcc tcagcgacgg gcatcgagac ggccagggct
      541 ctggggttcg cgctctccga gcggggggtg tgcgtggtga gcgggctcgc gctaggagtg
      601 gatgcggcgg cgcacgaggg agctgtcgag gccggtggcc ccacggttgg cgtgctcggc
      661 tgcggcatcg acgtcgtcta cccaagacgc aacaggggcc tcttccaatc ggtcagacgc
      721 cagggggccc tgatctccga gtactacctg ggagaggcgc ccctggcgtg gcgattcccg
      781 gccagaaacc gcatcatagc cgggcttgcc gggactgtgg tggtcgtcga ggcgcccgag
      841 caaagcgggg ccctcataac agcccgccac gccctcgacg caggacgcga tgtatgggcc
      901 gtgcccggtc ctccaggcgt gcacgagtgt aggggatcga acgggctgct cgccgacggt
      961 gcgggcgttc tgtgggacct acccgagttc gtcgatctcg tggcgcggga gcagagagtc
     1021 ccagtggcgg accgggcatc cggagacgtc cagcccctgc cctccggtct cgaggatacg
     1081 gaggctgccg tgctcagcgg ggttggcttc gaaccgagcg gggtcgacgc ggtcgcgaag
     1141 cgcagcggag tcgcgatgcc cgaggtgctc accgcgttag cgttgctcga actgaagggg
     1201 ttcgttagcc gtaacggtgt tggagcttac gtgaggaggg ccgtcccgtg agcaggcgca
     1261 cgctccgctc tgtatggtcg cactatttac gagtccgaaa gcgccttgcc gaagcggaga
     1321 tctcgggtgt ggagcggcgg atggccgaac gtgaggccag gggattgagg gaccggctcg
     1381 tggtcaacta ctccccgctc gtgaaatacg tcgccggcag gatctccggt cgtatgtccg
     1441 gaccgctcga tcaggaggac gtcgtcgcgt cggggctctt cgggctcctg aatgccgtcg
     1501 agacctacga tccagagcgc agcaccaagt tcgagtctta tgcgatctcc aggatccggt
     1561 ggtccatact cgatgagctg aggaaggtcg atccactgtc tcggcggact cgcatgagag
     1621 cgcaccagat cgagcgtacc agggacgaac tcgtgcagaa gctgggccgc gcgccgacgg
     1681 agtcagaatt ggtcggacgc ctggggatca gcgtaaccga acatcgggcg ttcatcgacc
     1741 aatacgccag ggcgcaggtg aggtcgctcg aagcccgcct ggagtaccag ggaggccctg
     1801 tcgccgggct gcacgacctc atcggggatc actcggcgac cgaccctgcg tccgccgcgg
     1861 agatggccga ggttcgcgcg cggctggttc gggccataga aggcctcggc gagcaagagc
     1921 gggtagttac caccttctac tattacgagg gccttacgct gagggagatc ggaagggtcc
     1981 tcgacctgac cgagggccgc atctcgcaga tactgcacag cgcgctcgtg cggctcaggc
     2041 aaaacctcgc tgaggagagc cggctctccg gaaggatgca ggggcgctag aagcatccac
     2101 ccccttgtgc taacatctgc gggatcggtc gaggctatgc tttcaggcct cgtccgcaac
     2161 tacgcacgct cgccgcaggg tccaccgggc ccgggtggtg gtccgccggg agatgcaagg
     2221 gcggattgcg aggcgcgggc ggaggatacc aaccacctta agagagaggt agagagatct
     2281 tgcagagcac aaccgagcgc gtaggaattc gagagctgct cgaggccggc gtccatttcg
     2341 gacaccaggc caaacggtgg aaccccaaga tgaagcgcta catcttcgcc cagcgggccg
     2401 gggtccatat tatcgacctg gatcagacgc tgacgttgtt ggagcgggcg cttagctttg
     2461 ttcgcggtgt cgctgaggct aaccgggaag tccttttcgt agggacgaag aagcaggccc
     2521 agatcccgct cgccgaggag gccatcagga gcgggcaacc ctttgttgcg gagcgctaca
     2581 taggcggcat gctcaccaac ttccagacga tccttccgcg catccagtac ttcaaagacc
     2641 tggccgtcag ggtcgacgag acccccgaag aggaaaggac aggcaaggac tggttcgcac
     2701 tgaacaggga gtaccagaag ctaaggcgca acttcgccgg cataaccgag atggagcgac
     2761 tccctggcgg tgtgttcgtc atagacccca aacgcgagga gctcctggtc aaagaggcca
     2821 acaggctcaa gatccctgtg ctggccctca ccgataccaa ctgcgacccc gaggtcatcg
     2881 actacg
//
LOCUS       DATMJA010000027         3635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_63497, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000027 DATMJA010000000
VERSION     DATMJA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63497"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1115)
                     /gene="gap"
                     /locus_tag="VNT12_00650"
     CDS             complement(114..1115)
                     /gene="gap"
                     /locus_tag="VNT12_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWI44645.1"
                     /translation="MAVRVGVNGFGRIGMLFTKAAMDRGDIEVVAANDLMPFDSLANL
                     FKHDSTHGIWHEDVSYDGNVLHVGDHEIKTFAERDPAQIPWGDVGADIVVESTGVFTS
                     RDGAAAHIEGGAKKVIISAPGKGVDATFVYKVNHEDYDPEAHEVVSNASCTTNCIIPM
                     VKVIQDNFGIDSGYMTTVHAYTNDQSLLDAAHKDPRRARSAPNNIVPTSTGAARTAGV
                     IYPELQGKVDGMSMRVPVADGSITDFVARISSEASADDVNAAFQSAADGELSDILEYA
                     EAPLVSSDIVGNKASCVFDSQLTMSNGGIVKVLGWYDNEWGYSNRTCDLAAYMGDKL"
     gene            complement(1197..2027)
                     /gene="whiA"
                     /locus_tag="VNT12_00655"
     CDS             complement(1197..2027)
                     /gene="whiA"
                     /locus_tag="VNT12_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HWI44646.1"
                     /translation="MRRQLAASAAANTRGVGRFELAGLVDASGVWDEEGVTLRTPSAA
                     VARSVVRLWRSEFGTESKLSPIDPDRFGRTRYAVRTAGNGAIQANEELRFARTARTAA
                     ERAGYLRGAFQGAGSVNRPGGRGGHHLEFVHREGEFIRRCVDFSRAPLKIVRRRGRWV
                     AYTKSSDGVTTVLAQMGLNEAVLEYEARAVLGEAKANANRVTNFDAANAGRTATAAAN
                     QQKALEALDPEHLSPALREMLELRIEYPDASLAELAHLGHLSKSAANHRLRRLVEIGR
                     "
     gene            complement(2029..2991)
                     /locus_tag="VNT12_00660"
     CDS             complement(2029..2991)
                     /locus_tag="VNT12_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HWI44647.1"
                     /translation="MNRIENIRVVAFGGGTGLPVLLRGLRDRVGDLTAVVTVADDGGS
                     SGRLRQELGVAPPGDVRNCLVALAGRRRLAEVFEYRFEAPGDLNDHSVGNIIIAALSD
                     MAGGFSEGVEQAARFLRIKGRVFPAATESLTLVVRYADGSITRGESVVHEVGKQVSEV
                     DVEPAGVPAPRGVIEAIQAADVLVLAPGSLFTSTIPALLGGGVRETLADFAGPVIYVA
                     NVMTQQGETSDFTVSDHVRAITEHVGPVVTDVLVHSGNLPPGTLARYEAEEASQVAVD
                     TEALHEMDLRVRKTYLLSKESRAGVRHDPSLLAREVAEAVLVRL"
     gene            complement(2988..>3635)
                     /locus_tag="VNT12_00665"
     CDS             complement(2988..>3635)
                     /locus_tag="VNT12_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWI44648.1"
                     /translation="DYTGRVTTPVLWDKQMGTIVNNESREIVRMLDTEFGEFATTDAH
                     FFPEDLRDETDATIDAIYEPVNNGVYRSGFATTQEAYEEAVTELFDALDHWEEVLSSR
                     RYLCGDRITEADWCLFPTLVRFDTVYHGHFKCNLRRIVDYPNLWGYLKDLYQRPGVAE
                     TVNMDHIQKHYYGSHESINPTRIVPKGPILDFTEAHDRGRFSDQTPQGNGYTRSV"
BASE COUNT          636 a         1149 c         1116 g          734 t
ORIGIN      
        1 tgtcagcatt tcagcttgtc agctttcgca cgggcaggtc tttgctgaca aggcggcgga
       61 gccgccgtgc tgaagtgctg acaagtcgcc gaaggcgacg tgctgaaatg ctcctagagc
      121 ttgtccccca tgtacgctgc cagatcacac gtcctgttcg agtatcccca ctcgttgtcg
      181 taccagccga gcaccttgac gataccgccg tttgacatgg tgagctgcga gtcgaagacg
      241 cacgaggcct tgttgcctac gatgtcgctg gagactagcg gggcttccgc gtactccagg
      301 atgtccgaga gttccccatc agcggctgac tggaaggctg cgttcacgtc atcggccgac
      361 gcttcgctgg agatgcgggc cacgaagtcc gttatggagc cgtcggcgac gggtacgcgc
      421 atagacatac cgtcgacctt gccctgtagc tctgggtaga tcacacccgc cgtcctggcc
      481 gcgcctgtcg acgtggggac gatgttgttg ggggccgagc gggcgcgcct cgggtccttg
      541 tgggcagcgt cgaggagact ctggtcgttg gtgtaggcgt gcaccgtggt catgtaacct
      601 gaatcaatgc cgaagttgtc ctggatcacc tttaccatcg ggataatgca gttggtcgtg
      661 cacgaagcat tggagacgac ctcatgcgcc tctgggtcgt aatcttcgtg gttcacctta
      721 tagacgaagg tggcgtcgac acccttgcca ggggccgaga ttatgacctt ctttgccccg
      781 ccttcgatgt gggctgcggc cccgtcacgg cttgtaaaga cgcccgttga ctcgaccacg
      841 atgtcggcac cgacgtcgcc ccagggtatc tgggccgggt cccgctcggc gaaggtcttg
      901 atctcatggt cgcccacgtg caggacgttg ccgtcataag agacgtcctc gtgccagatt
      961 ccgtgggtcg agtcgtgctt gaaaaggtta gccaggctat cgaaaggcat caggtcgttc
     1021 gccgcgacga cctcgatgtc gccacgatcc atcgcggctt tggtgaacag catcccgata
     1081 cggccgaagc catttacgcc tactcttacg gccactcttc tctccattct ctagcttgcc
     1141 ataaacttgt attttgcccg aaaatacgga agactaacct ccctttgcag ggctccctac
     1201 ctgcctatct ccaccaatct tctcagcctg tgattggccg cgctcttgga gagatgcccg
     1261 agatgggcga gttcggcgag ggaggcgtcg gggtactcga tccgtaattc gagcatctct
     1321 cttagcgcgg gggacagatg ctcgggatcg agggcttcca gggccttctg ctggttggct
     1381 gcggccgtcg cggtgcgacc ggcgttggcc gcgtcgaagt tggtgacgcg gtttgcgttg
     1441 gccttggctt cgccaagcac ggcccttgcc tcgtactcga ggacagcttc gttgaggccc
     1501 atctgggcga ggacggtcgt cacgccgtcg gagctcttgg tgtaggcgac ccaccgtccg
     1561 cgccggcgca cgatcttcag aggcgcacgg gagaaatcca cgcagcggcg gatgaactct
     1621 ccttcgcgat gtacgaactc caggtggtga cccccacgac cgcctggccg gttcacggag
     1681 ccggcgccct ggaaagcacc ccgcaggtag ccggcccgct ccgccgctgt gcgtgcggtg
     1741 cgggcgaacc tcagctcttc gttcgcctgt atcgccccgt tgcccgctgt gcgcaccgcg
     1801 tagcgggttc ggccgaagcg gtcggggtcg atcggtgaga gtttggattc tgtcccgaac
     1861 tccgagcgcc acaggcgcac cacggagcgg gcgacggcag cgcttggggt gcgtagagta
     1921 acgccttcct cgtcccagac gccagatgcg tcgaccagac cggcgagctc gaagcggcca
     1981 acacctcggg tgttcgccgc cgcgctggcg gcgagctggc gacgcatctc agaggcgaac
     2041 gaggacagcc tcggccacct ccctcgccaa cagtgagggg tcgtgcctga cacccgcgcg
     2101 gctctccttc gaaagcaagt acgtcttcct caccctcaaa tccatctcgt gcagggcttc
     2161 cgtgtcgaca gcaacctgcg aagcctcttc ggcctcatag cgggcgaggg taccaggagg
     2221 caggtttcca gaatggacga gcacgtcggt aaccacgggc ccgacgtgct ccgttatcgc
     2281 ccgcacgtgg tccgagacag taaagtcgct tgtctccccc tgctgcgtca tcacgtttgc
     2341 gacgtagatg acggggccgg cgaaatccgc cagggtctcc ctcaccccgc cgccgagcag
     2401 agcagggatc gtgctggtaa acaggctgcc cggagcgagc acgagcacgt cggctgcctg
     2461 gatagcctcg atcacccctc gaggcgcagg aacacctgct ggctcgacat cgacctcgga
     2521 gacctgtttt ccgacctcat ggaccacaga ttccccgcgc gttatgctcc catcggcgta
     2581 gcgcacgacg agcgtcaggc tctccgtcgc cgcagggaag actcgaccct tgattctcaa
     2641 gaatcgggcc gcctgctcaa caccctcaga gaacccgccg gccatatcag agagcgccgc
     2701 gataatgatg ttgcctacgc tgtggtcgtt caggtccccc ggcgcctcga aacggtattc
     2761 gaagacctcg gccagcctgc ggcgtcccgc tagcgccact aggcagttcc tcacgtcacc
     2821 agggggcgcg acccctagtt cctggcgcaa tcgtccgctg gagcccccat cgtcggcgac
     2881 cgtgacgacg gccgtaaggt ccccaaccct gtccctcaag cccctcagca acacgggcaa
     2941 ccccgtgccg ccgccgaacg ctacaaccct tatattctcg atcctattca cacagatcga
     3001 gtatacccat ttccttgtgg cgtctggtcg gagaagcgcc cgcggtcgtg cgcttcggta
     3061 aagtcgagga taggaccctt cggcactatg cgagtcgggt tgatgctctc gtggctcccg
     3121 tagtagtgct tctggatgtg gtccatgttg accgtctctg cgacgcctgg tctctggtag
     3181 aggtccttca gatagcccca caggttcggg tagtctacga tacgccgcag attgcacttg
     3241 aagtggccgt ggtagacggt gtcgaagcga acgagcgtgg gaaagaggca ccagtcggcc
     3301 tccgttatgc ggtcgccaca caggtagcgt cgggaggaga gaacctcttc ccagtggtcc
     3361 agagcatcga atagctccgt aacggcctcc tcgtaggcct cctgtgtggt ggcgaagcct
     3421 gagcgataga cgccgttgtt cacgggctcg tagatggcgt caatggtcgc gtcggtctca
     3481 tctcgcaaat cctcagggaa gaaatgtgcg tccgtggtgg caaactcgcc gaactcggta
     3541 tcgagcatgc ggacgatctc gcgcgactcg ttgttgacga tggtacccat ctgtttgtcc
     3601 cagagaacgg gcgtcgtgac gcgtcccgta tagtc
//
LOCUS       DATMJA010000028         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_300516, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000028 DATMJA010000000
VERSION     DATMJA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300516"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VNT12_00670"
     CDS             complement(<1..278)
                     /locus_tag="VNT12_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HWI44649.1"
                     /translation="MFSSVRRVVSQVRFWLSALRRLGFRIFLALTALQALIVGVLVAM
                     AEFRKRRRGPREGFPWEEQPEIELETGEDRLKIFAYGVDLYGAMLEEI"
     gene            complement(321..986)
                     /locus_tag="VNT12_00675"
     CDS             complement(321..986)
                     /locus_tag="VNT12_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HWI44650.1"
                     /translation="MAFPPFEERVISPGSSTAFPEIVAPVSETGTTIAGKAVEDPGEI
                     GLPSRYGKDLVDDLTVRIVRAMDEDYLVALAKGYPPFRRAVCEEITRNNARQNAVIGA
                     LPIPGADMPVMTANQARMVLNIAAAYGEELSMDRAKELLAVLAGGFGLRALSRQAVKL
                     VPVGGWAASAVIAYAGTVAMGRSSILYFERGGQRIGEKEMAEIRTRALKEGKSFVSRL
                     RRK"
     gene            985..1491
                     /locus_tag="VNT12_00680"
     CDS             985..1491
                     /locus_tag="VNT12_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HWI44651.1"
                     /translation="MLEELAPRIVLALDEDYLVALAKGYPPLRRAVCEEIVRNNARQN
                     AVIGALPIPGADMPVMTANQARMVLNIAAAYGEELSMDRARELLGVLAAGFGLRALSR
                     QAVKLVPVGGWAASAVIAYAGTVAMGRTSILYFERGKQEIGAAEMARIRSRAIEEAKA
                     FVSRLRRK"
     gene            1565..2845
                     /locus_tag="VNT12_00685"
     CDS             1565..2845
                     /locus_tag="VNT12_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HWI44652.1"
                     /translation="MFSLLRRVLSEMRSWLSTVRRLGLRIFLALTALQVVTVGVLVAM
                     AEFRKRRQGPREGFPWEDQPEIRMESGKDRLKIYAYGVSLYEAMLDEIEGAQDEIFVG
                     TFIWKGDEVGRRFVDALARKAREGVDIYVVFDGLANMFVPPSFKRFPERIHRLHFRPL
                     SGPAKILNPRNVFRFHRHVMSVDGRVAFLGGYNIGSLYAAGWRDTHVRVRGDAAREVD
                     NAFIDFWNAHRTEELPSIAPNKDRDWNPAVRVRRNDPYMRIFPIREMYIEAIDRANSH
                     VYLTHAYFVPDRAMKAHLIDATERGVDVQLLVPQQSNHVTADWLARRHFYDLLKNGVR
                     IFLYRHIMIHSKTATADGLWSTIGSANIDRLSMLGNYELNIEVYSRRLAAQMEEMFEM
                     DKTNAEEVTLEEWERRPLPAKLVERTLASLSPLV"
BASE COUNT          562 a          897 c          926 g          542 t
ORIGIN      
        1 tcgatctcct cgagcatcgc cccgtagagg tctaccccgt aggcaaagat cttgagcctg
       61 tcctcgcccg tctccagttc gatctcgggc tgctcctccc acgggaaccc ctcccgcggc
      121 ccccgacgcc gcttgcgaaa ctcggccatc gcgacgagca cgcccacgat cagcgcctgc
      181 aaagccgtca aggcaaggaa gatcctgaaa ccgagcctgc gcaacgcgga gagccagaag
      241 cgcacctgtg atacgaccct ccgcacggaa gagaacatga gatcgattat attagaaggt
      301 atcaggcagc agcccgaaac ctacttgcgg cgtagccgag agacgaagct cttgccctcc
      361 ttcagggctc gggttcggat ctctgccatc tccttctcgc ctattctctg gccgccgcgc
      421 tcgaagtaga ggatgctgga gcggcccatg gcaacggttc cggcgtaggc tattaccgcc
      481 gaggccgccc atcctcccac cggcacgagc ttgacggcct ggcggctcag ggccctgagg
      541 ccgaagccgc cggcgagcac ggcgaggagc tccttggcgc ggtccatcga gagctcctcg
      601 ccgtaggcgg cggcgatgtt aaggaccatc cgagcctggt ttgccgtcat cacaggcata
      661 tcggcgccgg ggatgggcag cgccccgatc acggcgttct ggcgggcgtt gttgcgcgtg
      721 atctcctcgc atacggcccg cctgaacggc gggtagccct tggcgagcgc gacgagatag
      781 tcctcgtcca tcgcacgcac gatgcgcacc gtcaggtcgt cgaccagatc tttcccgtac
      841 ctggagggca gtcctatctc gccagggtcc tccactgcct taccggcgat ggtggttccc
      901 gtctccgaga caggggctac tatctcgggg aaggccgtcg aggaccctgg tgagataact
      961 ctctcttcga aaggtgggaa ggccatgctc gaagagctgg ccccacgcat cgtgctggcg
     1021 ctcgacgaag attatctggt cgcgctagcg aaggggtatc ctccgctcag gcgggccgtt
     1081 tgtgaggaaa tagtacgcaa caatgcccgc cagaacgccg tgatcggggc gctccccatc
     1141 cccggggcgg acatgccggt gatgacggca aaccaggcga ggatggtgct caacatagcg
     1201 gccgcttacg gggaggagct gtcgatggat agggcgcggg agctcctcgg ggtgctcgcc
     1261 gccggtttcg gcctgagggc cctgagccgc caggccgtga agctcgtgcc tgtgggggga
     1321 tgggccgcct cggcggttat cgcctacgca ggtaccgtgg cgatgggccg taccagcatc
     1381 ctctacttcg aacgcggcaa gcaggagata ggtgcagcgg agatggccag gatcaggagc
     1441 cgagccatcg aggaggcgaa ggccttcgtc tcgcgcctgc gccgaaagta ggtatcaggc
     1501 tgaacttcga cggctcctgc gggagggtcg gccttttcgc tgagcgctct atataatcga
     1561 atctatgttc tcactcttgc ggcgggttct ttcggagatg cgttcttggc tctccacggt
     1621 gcgcagactg ggattgagga tcttcctcgc tttgacggcg ttgcaggtgg tgaccgttgg
     1681 tgtgctcgtc gcgatggccg aattccgcaa gcgccgccag ggtccgcggg agggattccc
     1741 gtgggaggac cagcccgaga taagaatgga gtcaggcaag gaccggctga agatctacgc
     1801 ttacggggtc agcctctacg aggcgatgct cgacgagatc gagggcgcgc aggacgagat
     1861 cttcgtcggg acgtttatct ggaagggtga cgaggtcggg cgccgttttg tggatgccct
     1921 ggcccgtaaa gctcgcgagg gtgtggatat ctacgtcgtc ttcgacgggc tggccaacat
     1981 gttcgtgccg ccttctttca agaggtttcc agaaaggata caccgcctcc acttccgtcc
     2041 tctgtcgggg ccggcgaaga tactgaaccc gaggaacgtc ttcagatttc accgccacgt
     2101 gatgagcgtt gacggcaggg tggccttcct cggcggctac aacatcgggt ccctgtacgc
     2161 agcggggtgg cgcgacacac acgtcagggt gcgtggcgat gctgccaggg aggtggacaa
     2221 cgctttcatt gacttctgga acgcccaccg cacggaggag ttgcccagta tagcccccaa
     2281 caaggatcgt gactggaacc ctgccgtaag ggtgcgccgc aacgaccctt acatgaggat
     2341 ctttcccata agggagatgt acatcgaggc gatagaccgc gccaactcac acgtctatct
     2401 gacccacgcc tatttcgtcc ccgacagggc gatgaaagcg cacctgatag acgcgacgga
     2461 gcgcggcgtc gacgtacagt tgctcgtccc ccagcagtcc aaccacgtca ccgcggactg
     2521 gctcgcgcga cgccacttct acgatctcct gaagaacggt gtcaggatct tcctttacag
     2581 gcacatcatg atccactcca agaccgccac cgctgacggc ctgtggtcga cgataggcag
     2641 cgcgaacatc gaccgtctca gcatgctcgg caactacgag ctcaatatcg aggtctacag
     2701 ccgcaggctc gcagcccaga tggaggaaat gttcgagatg gacaagacca acgccgaaga
     2761 ggtgaccctg gaggagtggg aacgccgtcc actccccgcc aagcttgtcg agcgtaccct
     2821 cgcgagcctt agcccgctgg tatagaaagc atcaggtttt gagatcgggt ccccgaccgc
     2881 gccggcagag gattaagtgt gcttagtgct tagtataatg acgatac
//
LOCUS       DATMJA010000029         4427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_655907, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000029 DATMJA010000000
VERSION     DATMJA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655907"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..2784
                     /locus_tag="VNT12_00690"
     CDS             82..2784
                     /locus_tag="VNT12_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWI44653.1"
                     /translation="MPSVKHTSPFLRYGVTVLAVGVAFAIKLLLDPLIEQDVPFLLVF
                     GAIMVSAWYGGLGPGLLATVAAGLTTDYFFLDPKGTVSGFSLETVPLLVFFLEGTLVC
                     LLVEALRAARSRAEGSTLEAERQREHLRRNEEHFRSLVEGMRNYAIFTLDPKGRVASW
                     NAGAERLEGYSSEEVVGEHFSMFFTEEDVISGRPERLLEAAGEGRTEEENWFVRKDGS
                     RFWAGTILTALHDDEGELRGFSEVTQNITEKKEAERLRKEAENRLRTLVEHVPVITYT
                     AQIGGDHALTYVSPQIKRVLGYSPGEMVADPDLWTKMLHPHDRRWVLAENRRTGETGD
                     PFALEYRMFARNGSVVWLRDEATLVRDEEGNPLRWQGFMLDITKRKKAEERLREREEL
                     YRNVVEQAAENIFLFDPYTGQILEANASFHQSLGYEAEELRRLTLYDIVAHDRESIDR
                     NIRLVLEEGRHAIGERRYRRKDGSLIDVEVNAGAIAYGGRPALCVVAHDVTQRKEAEE
                     ALRRSLDALLALYETGQILSSSLEREEIGSRLLEVVRRVSNLTAAVISVPDEKGELSI
                     WRSSGLDGLWRQARFDPEALSARRKVLETGEHQLLRLRRPEDPQEERNLVSMNLPLLV
                     RDRVIGVLEAYGTDALGEAGAENLYSLANQAASALENARLYAELAERENQLRRLVGKL
                     IMAQEEERRRVAYDVHDGLAQTAAAAHQHLQLFARHNPPGSASGQEELDEALELVREV
                     VGEARQVIHDLRPTVLDDFGLAAAVRLQVQTLRSEGLEISLEEALGDERLPPEVETTL
                     FRVIQEALTNVRKHARASTVRVVLDHPGRAVRLQVTDDGRGFEPDEATRSNGPGERVG
                     LSGMRERLSLLGGRFEAHSELGSGTTVTAEVELPTNREDSDHAV"
     gene            2774..3466
                     /locus_tag="VNT12_00695"
     CDS             2774..3466
                     /locus_tag="VNT12_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI44654.1"
                     /translation="MQYETKRLVLADDHHLLRRGFRSLLSSEPGLEVVGEASTGLEAI
                     ELCRRLAPDLVLMDVRMPEMDGITATRRIKHEQPGVSVLMVTMHENPDYLLEALDAGA
                     AGYVLKDAPADRLINAVRRTLEGESPLNQELATRLLRQLAEEKQHNLYRAPSKRPEPL
                     EDALTPRETEVLGLLTTGQTNQQIAQTLTISKGTAKVHVERIIRKLGVSDRTQAAVRA
                     IELGFTSLETRR"
     gene            3591..3953
                     /locus_tag="VNT12_00700"
     CDS             3591..3953
                     /locus_tag="VNT12_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44655.1"
                     /translation="MLRPARGPATIAILGGNPVIGKALESLLASADYVARFFSEYPEG
                     DAEPLRGARVALLLPALSGGRQEALKAQIKNTPSTMNLPVIELTTTPIDNSQSILVPW
                     PCSLEDLRRTIEETLQNS"
BASE COUNT          915 a         1346 c         1438 g          728 t
ORIGIN      
        1 caacgagagg taaacatggg aaccgctaca cgcacgccga acggtaaacg acactctgct
       61 ttcggcgggt acacggtcta gatgccctcc gtaaaacata cgtcgccgtt cttgcgatac
      121 ggcgttaccg tgctggccgt cggggtggcg tttgcgatca agctgctact cgacccgctg
      181 atcgagcagg atgtgccttt cctgctggtc ttcggcgcca ttatggtcag cgcgtggtac
      241 ggggggcttg gacccggcct gctcgctacg gtcgcggcgg gcctgaccac cgactacttc
      301 ttcctcgacc cgaagggcac cgtctcgggg ttcagcctgg agaccgtgcc gcttctcgtg
      361 ttcttcctgg aagggacgct cgtctgcctg ctagtcgagg ccctgcgcgc cgcgaggtcg
      421 cgagcggagg gtagtacgct ggaggccgag cgccaaaggg agcacctgcg caggaacgag
      481 gaacacttcc gctcgctggt ggaaggcatg cgaaactacg cgatcttcac gctcgacccg
      541 aagggacgcg tcgccagctg gaatgctggc gccgaacgcc tcgagggcta cagctccgag
      601 gaggtcgtcg gagagcactt ctccatgttc ttcaccgaag aggacgtaat tagtggcagg
      661 cctgagagac tcctcgaggc agcgggcgaa ggccggaccg aagaagagaa ctggtttgtg
      721 cgcaaggatg gatctcggtt ctgggcaggc acgatcctga cggcgttgca cgatgatgag
      781 ggagagctgc ggggtttctc ggaagtcacg cagaacatca cggagaagaa ggaggcggag
      841 aggcttcgca aggaggccga gaaccgccta cgcacactcg tcgaacacgt gcctgtgatc
      901 acctacacgg cgcagatagg cggcgaccac gctctgactt acgtcagtcc gcagatcaaa
      961 agggtcctcg gctactcacc aggcgagatg gtggcggacc ccgacctctg gacgaagatg
     1021 ttgcatcccc atgacaggag gtgggtgctg gccgagaaca ggcgtaccgg tgagaccggt
     1081 gatccgttcg cgctggagta ccggatgttc gccaggaatg gaagcgtggt ctggctgcgc
     1141 gacgaggcga ctctcgtgcg cgacgaagag ggtaatcccc tccgctggca gggcttcatg
     1201 ctcgacatca ccaagcggaa gaaggccgaa gagaggctgc gcgagcgcga ggagctgtac
     1261 cgcaacgtcg tcgagcaggc cgccgagaac atcttcctgt tcgatccgta tactggacag
     1321 atcctcgagg cgaatgcctc cttccaccag tcgctcggct acgaggccga ggagcttcgg
     1381 cgcctgacgc tctacgacat cgtcgcccac gatcgagaga gtatagatcg caacatcagg
     1441 ctcgttctgg aggaggggcg tcacgctatc ggggagaggc gctacaggcg caaggacggc
     1501 tctctaatcg acgtggaggt aaacgccggc gcgatcgcct acggcggccg ccccgcgctc
     1561 tgcgtcgtcg cccacgacgt gacgcaacgc aaagaagccg aagaagcgct gagacgcagc
     1621 ctggacgcct tgctcgccct ctacgagaca ggccagatcc tgagctccag cctggaacgg
     1681 gaggagatcg gctcgcgtct gctcgaggtc gttcgccggg tctcgaacct caccgccgcg
     1741 gtcatcagtg tcccggacga gaagggggag ctgagcatct ggcgttcctc gggactggat
     1801 gggttgtggc gccaggctcg cttcgaccct gaggccctct ccgcccgccg caaggtgttg
     1861 gagaccgggg agcaccaact cctcaggctg cggcggccgg aagatccgca agaagaacgg
     1921 aacctggtca gcatgaacct cccactgctg gtaagggaca gggtcatcgg tgttctcgag
     1981 gcctacggta ccgacgccct gggcgaagca ggagcggaga acctgtacag cctcgcaaac
     2041 caggccgcga gcgccctgga gaacgcacgg ctctacgcgg agctggccga gcgtgagaac
     2101 caactccggc gcctggtcgg gaaactgata atggcccagg aagaagagcg ccgccgcgtc
     2161 gcctacgacg tacacgacgg cctcgcccag acggcggcgg ccgcccacca gcaccttcag
     2221 ctcttcgccc gccacaatcc tcccggttcg gccagtggcc aggaggagct cgatgaggcg
     2281 ctcgagctcg tcagggaggt ggtcggggag gctcggcagg tgatccacga cctgcgtccc
     2341 acggtgctcg acgacttcgg cctggccgcc gccgtccgcc ttcaggtcca gaccctccgg
     2401 tctgaaggtc ttgagatcag cctggaagaa gcgcttggcg atgagcgtct gccacccgag
     2461 gtggagacaa ctctcttccg ggtcatccaa gaggccctga cgaacgtacg caaacatgcg
     2521 cgggcctcca cggtgcgcgt ggtgctcgac cacccgggac gtgcggttcg cctgcaggtc
     2581 acggacgacg gacgcggctt cgaaccggat gaggccacga gaagtaacgg tcccggcgag
     2641 agggtgggcc tctcgggcat gcgcgagagg ttatccttac tgggcgggag gttcgaagcc
     2701 cacagcgaac taggatccgg cacgacagta acggcggagg tagaactgcc gacgaataga
     2761 gaggactccg accatgcagt atgaaacaaa gcggctcgtc ctcgccgacg atcaccacct
     2821 tctcagaaga ggattccgga gcttgctgag cagcgaacca ggactggagg tggtcggcga
     2881 agcgtcgact gggctggagg ccatcgaact ctgccggcgt ctggcgccag atctcgtact
     2941 tatggacgtc cgcatgcccg agatggacgg catcacggcg acccgccgga tcaaacacga
     3001 gcagcccggc gtaagcgtac tcatggtgac catgcacgag aatcccgact acctgctcga
     3061 agcgctggat gccggcgccg ccggctacgt gctcaaagac gcccccgcag accgcctgat
     3121 caacgcggtc cgcagaactc tggagggcga gtctccactc aaccaggaac tggccacacg
     3181 actgctcagg caattggccg aagagaaaca gcacaacctg tatcgtgctc cgtctaaacg
     3241 ccccgagcct ctggaagacg cgctgacccc ccgcgagacg gaagtcctcg ggctcctgac
     3301 taccgggcaa acaaaccagc agatcgcaca gacactgacc ataagcaagg gaacagccaa
     3361 ggtgcatgtg gagcggataa tccgcaaact aggggtctcg gatcgcaccc aggcggccgt
     3421 gcgcgccatc gaattaggat tcacttctct cgagacaagg cggtaacctt atcccttaag
     3481 ataaggttcg tatgccactc ggcatacgta agataggcca tacggcgtac gcgccgacaa
     3541 ccccttccac cgtataattg gcagatggac gtgggcacag ggatctgagc atgctgagac
     3601 ctgcccgagg gcccgcaacc atagccatcc tgggcggcaa ccccgtgatc ggtaaagctc
     3661 tcgagtcgct gctcgcctcc gccgactacg tggcacgatt cttttccgag taccctgaag
     3721 gagacgcaga gcccctcaga ggggcgaggg tcgcgctcct cttgccggcc ttgagtggcg
     3781 ggcgtcagga agcgctcaaa gcccagatca agaacacccc atcgacgatg aacctgcccg
     3841 tgatcgaatt gaccaccacc ccgatcgata acagccagag tatcctggtg ccgtggccct
     3901 gcagcttaga agacctgagg cgcactatcg aggaaacgct gcaaaacagc taatcagcac
     3961 gtcgcccggc ttcgccaggc gacttgtcag cacgcgccct gtcgggcgct tgtcagcact
     4021 tcagccagta aacactccag cattcgcaag cccttgctga cttgctgaaa gccgccgact
     4081 gaagggaggc ggcgtgctga caagcgaagg cgcagcctga gcgtgctgaa atgctgacac
     4141 gctggtatat gccgttaaca catacgtttc gtttgcttgt ataattcgtg tgaccggcgc
     4201 tcgctccgat gagagtcagg tgggtgtcgg tgattggcat tgtttggtgg caggatgagg
     4261 aggtttcggt tgacggagga gagaggaacg gtgtccggca tggaccctgc cgagctctgg
     4321 cgccagtgga tggagtcggg gcgcaaggta tggtcggaga tggccggcgc ccaggggcgg
     4381 caggtcgacc cttacggcgc ataccgccgg tggttcgaag gtgtgcg
//
LOCUS       DATMJA010000030         3305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_4619, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000030 DATMJA010000000
VERSION     DATMJA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4619"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..624
                     /locus_tag="VNT12_00705"
     CDS             187..624
                     /locus_tag="VNT12_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWI44656.1"
                     /translation="MDGRERVAEFGRELREGPEPSDRSIKEIIDALRPQLQELVNKQV
                     ELAKTEMAPVAKRAGLASGLLAAAGIFMFVFLIILSLAGVYILDVFLSLWVSALIVSG
                     ILLLVGGILAASGAGILRRLDPTPHRTIRTLQQNINWIKGQLR"
     gene            624..971
                     /locus_tag="VNT12_00710"
     CDS             624..971
                     /locus_tag="VNT12_00710"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HWI44657.1"
                     /translation="MSEIPERIEREMFEIRSRMAPDVRDLKKHAEPQVIVKRTSTKVK
                     ERVRDAVIRFGKSLKESASRQAKMIGEAGRKRNPAPLTDAVKSDPRPLILLAISLAVT
                     LMAVRKISNGREE"
     gene            1044..2222
                     /gene="larC"
                     /locus_tag="VNT12_00715"
     CDS             1044..2222
                     /gene="larC"
                     /locus_tag="VNT12_00715"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HWI44658.1"
                     /translation="MIHVHFQPVGGVAGDMTLAALVAAGASLPEITAPLRGLGVTFDL
                     AVERVEVNGIGTLKAAVEYPDEHPHRTFRDIRASIEAAELPERVSSRALEAFRLLAVA
                     EGAVHDEDPEEVTFHEVGAVDSIVDVVGSCVALELLDAASVSCGPLPMGTGVVDTAHG
                     LLPVPGPATLEILEGSSVRWTDEPIEMTTPTGAALMRSFTGGKFTDAAPPMTLHATGY
                     GAGRARLRSAPNLLRAVVGELEGPEGAVELLEANVDDASGELLGAAVERLLAAGALDA
                     WLEPIVMKRGRGAYKACALALSSDRERLGRLLMRETGTLGVRHHTVGRTVAGRRHVVV
                     DLPYGKCRLKVGSIDGEDFIAAPEYTDAVRLAGETGFPLPRVYEDAKAAFGGCGQHFS
                     "
     gene            complement(2606..3247)
                     /locus_tag="VNT12_00720"
     CDS             complement(2606..3247)
                     /locus_tag="VNT12_00720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWI44659.1"
                     /translation="MVRVVVLYVDGTLMDTNYLHTEAWARAFEKVGHRVPRVRLHKEV
                     GKGAGLLIREFIDDEETVEKIQDLHSEYYAELQEYGHPLPGAKELISSLKGRGYEVWL
                     VTSAEDEELEHHMQELEAEDSVDGIVNSSAVENPKPAPDIFEEALRRAGATADETVAV
                     GDAVWDLEAAREAGIRTVAVLTGGAYHKEALEAAGAVAVYEDCAALLESDFPE"
BASE COUNT          625 a         1032 c          998 g          650 t
ORIGIN      
        1 ttcatgttac atcatcgatt tacttcgcgt gctttatgac atgttggata ctaagatgtg
       61 gtgtaggata gggatggttg ttcccgggag acctaccgga ttacataggg catggcaagg
      121 gtaactaaga gcgggtgaaa ggcaagaaaa ctcattgttt caggatcaga agaaaggagc
      181 gtggggatgg atgggcgaga gcgggtagcg gaattcggca gggagcttag ggagggtccc
      241 gagccttctg accggagcat caaggagatc atcgacgctc tgaggcccca gcttcaggag
      301 ctcgtcaaca agcaggtcga gttggcgaag acagagatgg cgcccgtagc caagagggcc
      361 gggttggctt ctggtcttct cgcggctgcc ggaatcttca tgttcgtctt cctcataatc
      421 ctctcccttg ccggggttta catactggat gtcttcctct cactgtgggt ctcggctctg
      481 atcgtcagtg gtatactgct gctggtcggt gggatactcg ccgcgtccgg cgctggcata
      541 ctgaggaggc tggatcccac gccccaccgg acgatccgta cccttcagca gaacattaac
      601 tggataaagg ggcagttgag gtaatgagcg agatacccga gcgcatagaa agggaaatgt
      661 tcgagatccg gagccgcatg gcgcctgacg tgagggacct caaaaagcac gccgagcccc
      721 aggttatcgt gaagcggacc tctacaaagg tcaaagaacg cgtcagggac gccgtaatcc
      781 gctttggcaa gagcctcaag gaatcggcca gtcgccaggc gaagatgatc ggggaggcgg
      841 gcaggaagag gaaccctgcc cccttgacgg acgccgtgaa gagcgacccg aggcccctga
      901 tcctgcttgc catcagcctg gccgtcacct tgatggccgt gcgcaagatc tcaaacggac
      961 gagaggaata gaggggcggt tcccctagcc ccgaatactc ccacctgctt gagaccggtc
     1021 accacaaggt caagggaccg gttttgatcc acgttcattt ccaacccgtg ggtggcgtgg
     1081 ctggcgacat gaccctcgcc gcgctcgtcg ctgcgggtgc gtcgctgcct gagatcacag
     1141 cccccctgcg cgggctcggc gtaaccttcg acctcgctgt ggagcgtgtc gaggtgaacg
     1201 gtatcggcac actcaaggca gccgtcgaat accctgacga gcacccccac agaaccttca
     1261 gggatattcg cgcctctata gaggccgccg aactgccgga gagagtctcc tcacgcgccc
     1321 tggaggcttt ccgccttctg gctgtcgcgg aaggcgccgt ccacgacgag gaccccgagg
     1381 aggtcacttt ccacgaggtc ggggcagtgg actcgatagt cgacgtggtc ggtagctgtg
     1441 tggcgctgga gctgctcgat gctgcctccg taagctgcgg tcctcttcct atgggcacgg
     1501 gtgtcgtcga taccgcccat ggtctcctcc cggtgcctgg accggcgacg ctggagatcc
     1561 tcgagggctc gtctgtccgc tggaccgatg agcctatcga gatgaccacc ccaacggggg
     1621 ccgcgctcat gagatccttc accggcggca agtttacgga cgcggctccg cccatgaccc
     1681 ttcacgccac gggttacggg gccggaaggg cacgcttgag gagcgcgcct aacctgctgc
     1741 gcgccgtcgt cggggagctc gaagggcctg agggtgccgt cgagttactc gaggccaacg
     1801 tcgacgacgc ctcaggcgag cttctcggag ctgcggtgga gcgtctcctc gcggccggcg
     1861 ccctcgacgc ctggctcgaa cccatcgtga tgaagcgtgg ccgaggcgct tacaaggctt
     1921 gcgccctggc cctctccagc gaccgcgagc gtctcggcag gctcctcatg cgggagaccg
     1981 gaaccctcgg cgtgcgtcac cacacggtcg gccgcaccgt cgcaggacgc cgccacgtcg
     2041 tggtcgacct tccctatggt aagtgcaggt taaaggtggg aagcatcgac ggtgaagact
     2101 tcatcgccgc ccccgagtac actgacgccg tcaggctcgc cggggagacc gggttcccac
     2161 tgcccagggt gtacgaggat gcaaaggccg ccttcggcgg ctgcggtcag catttcagct
     2221 agtcagcatt tcagcacggc ggctccgcct ccttgtcagc atctcagcaa gcacctgcta
     2281 gagcgaggct tccacacgca gccaggcttc tcggcaaacg atgcggcgct aattcaccgc
     2341 agagagagca cggccgactc aagcagcttc ctaacaaagc tgaaattctg agctgcgcgt
     2401 gtttggcaag atgaagctgt agcccttctc gtcgctgaac cattttaccg ttccctgggc
     2461 catacccttt gcgccgacct ttcacttcct gagcccgatc tttggatgac agacctgcgg
     2521 gtatgccccc caacaaacag cctctcttcc agcttgcgtg tggcactctt gtacaaaact
     2581 cccaggcttt cacgcgaacc cggcgctact cagggaagtc ggactcaagg agtgcggcgc
     2641 aatcttcgta gacggccaca gctccggccg cttcgagggc ctccttgtgg taggcgccgc
     2701 cagtgaggac ggcgacggtg cgtatgccgg cttccctagc agcctcgagg tcccataccg
     2761 cgtcaccgac ggcgacggtc tcgtcggcgg tcgccccggc tctccgcaag gcctcctcga
     2821 agatgtcggg ggccggtttc gggttctcga cggcactcga gttgacgatg ccgtccacgc
     2881 tgtcttccgc ttcgagctct tgcatgtgat gttccagctc ttcgtcttcg gcgctggtaa
     2941 ccaaccagac ttcatagccg cgtcctttga gcgaggagat caactccttc gctcccggaa
     3001 gcgggtggcc gtactcttgc agctccgcgt agtactcgct gtggaggtcc tggatcttct
     3061 ctaccgtctc ctcgtcgtcg atgaactccc ggatcaaaag ccctgcgccc ttaccgacct
     3121 ccttgtggag cctgaccctc ggcacccgat ggcccacctt ctcgaacgcc cgcgcccacg
     3181 cctctgtgtg caggtaattc gtgtccatca acgtgccgtc aacgtaaagt acgaccactc
     3241 tcaccatcca gactcctctc gtgccttaga atagactcat gaagtattcg aaagatcccc
     3301 aaaac
//
LOCUS       DATMJA010000031         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_4853, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000031 DATMJA010000000
VERSION     DATMJA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4853"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1559)
                     /locus_tag="VNT12_00725"
     CDS             complement(183..1559)
                     /locus_tag="VNT12_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWI44660.1"
                     /translation="MEEGAARGRSVSRLNILIAITAAIIGLIYGYDLGSIASALLFLV
                     PAFDLSTTMTSVVTSAVVLGQLFGALFAGRISNTIGRKRSLVLVALGYAAFAGLQGLA
                     PNEWFLTVVRFLLGLAIGISIVVAPAYIAESAPVRVRGSMLVTFQIATTSGIAIAYFV
                     GAALAGTESWRLILSLSAIPALIVLALIVRLPDTARWLLMNGQREEAADLLRRVEPDM
                     DPDEEVRRIEHDLSYEEKGSFAELFQGRFRKAGIFVVGLGFLVQITGINAIVYYSPTI
                     IQEVGVTSPTGAIIATGFVQIAGVIAEVVAFLVVDRWGRRPTLLTGIGMMGAANAILV
                     LAYALNSPATFTLLGILLFTVGFNFGYGSLVWVYASESFPVRLRTQGGSAMLTADLFA
                     NFIVGVVFLGALGALGGSVTFGIFFALALVSFAFVYVLAPETKGRPLEAIRSYWYNGG
                     RWPEKTST"
     gene            1689..>2209
                     /locus_tag="VNT12_00730"
     CDS             1689..>2209
                     /locus_tag="VNT12_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44661.1"
                     /translation="MHVYSDQASERHVNDLAGVESPKRFAAALAGVERAEQSGVFVER
                     RPCSPAEREALTAVHDQAYLDNLREMSVTGGGYLSLDTAVGVGSWEAASLASGAACSA
                     VESAIAGETAFAVARPPGHHAGRDYGMGFCLTNHAAVAAGHVLSRGLERVAILDWDVH
                     HGNGTQDIFYGDGR"
BASE COUNT          458 a          657 c          755 g          339 t
ORIGIN      
        1 atgggctaga ccagcaggcg atgtgcatag taacttgaag gcccgaccga tccgagatgc
       61 gatctcacag cgatctcaca gcgctcttca taagagccga gccataagac ggcagagcag
      121 ctctccttag agaggtgact ctcccgtgca agacctgatc tgcgcggcga acctatctcc
      181 gcttacgtgc tggtcttctc gggccagcgt ccgccgttgt accagtagga gcggatcgct
      241 tccagcggcc gtcccttggt ctcgggggca agtacgtaga cgaacgcgaa cgagaccagt
      301 gccagggcaa agaagatccc gaaagtaacc gagccgccta aagcgcccag ggcgccgagg
      361 aacacgactc cgacgatgaa gttggcgaac agatctgctg tcagcatggc tgagccgccc
      421 tgggtgcgca ggcgcacggg gaagctctcg gaggcgtaga cccagaccag cgaaccgtaa
      481 ccgaagttga agcctacggt gaagagcaag atgccgagca aggtgaacgt cgcaggggag
      541 ttcagcgcgt aggccagcac gaggatggcg ttggcggccc ccatcatgcc gatcccggtc
      601 agaagcgtgg gccgccttcc ccacctgtct acgacaagga aggcgacgac ctcggcgatt
      661 acgcccgcaa tctggacgaa gccggtcgct atgatcgcgc ccgtcggact cgtgacgccg
      721 acctcctgga tgatcgtcgg actgtagtag acgatggcgt tgatacctgt gatctggacc
      781 aggaaaccaa ggcccacgac gaagatgccg gccttacgga aacgcccctg gaagagctcg
      841 gcgaagctcc ccttctcctc atagctaagg tcgtgctcga tcctccggac ttcctcatcg
      901 gggtccatgt cgggttcgac cctgcgcagg aggtcggccg cctcctcgcg ttggccgttc
      961 atcaggagcc agcgggctgt gtccggaagg cggacgatga gggccaacac gatgagggcc
     1021 gggatggcgg agagggaaag tatgagacgc cagctctcgg tgcccgcaag ggccgctccg
     1081 acgaagtagg cgatggcgat tcccgaggtg gttgctatct ggaaggtgac gagcatcgag
     1141 cccctgacgc ggacgggcgc ggactcggcg atgtacgccg gcgccacgac tatggagatg
     1201 ccgatggcga gaccgaggag gaacctaacg acggtcagga accactcgtt gggggccagt
     1261 ccctgcaggc cggcgaacgc cgcgtagccg agcgcgacca gcaccaggga gcgcttgcgc
     1321 ccgatggtgt tggagatgcg cccagcaaag agcgccccga acaactgccc gagcacgacg
     1381 gctgaggtaa cgacggaggt catggtagtg gagaggtcga aggcggggac gaggaagagg
     1441 agcgcgctcg cgatagagcc caggtcgtag ccgtagatca ggccgatgat cgcggccgtg
     1501 atggcgatga ggatgttgag cctcgatacg ctccgcccgc gcgctgcccc ttcttccacc
     1561 gcaaaccttc tttccccagg ggtcccatcc gcgatgctgg catagtagca tagtggagga
     1621 ggatcgagga gacaggagag gatctataca aagcggagac ggaagcgtga cctgggagcg
     1681 tgaaacgttt gcacgtatac agtgaccagg cctcggagcg ccacgtcaac gatctcgccg
     1741 gcgtggaatc cccgaagagg ttcgctgcgg cccttgcagg ggtcgaaagg gccgagcaaa
     1801 gcggcgtatt cgtggagcgg cgcccatgta gtcccgcgga gagagaagcc ctcactgctg
     1861 tccacgacca ggcttacctg gacaacttgc gggagatgtc cgtgacggga ggaggctacc
     1921 tcagccttga cacggccgtc ggcgtgggtt cctgggaggc ggcctcgcta gcgagcgggg
     1981 ccgcctgctc ggcggtcgag agcgccatcg ccggcgagac ggctttcgcc gtcgcccggc
     2041 ctccaggaca ccacgcaggg agggactacg ggatggggtt ctgcctcacg aaccacgcag
     2101 ccgtcgcagc cgggcacgtc ctttcgagag ggctggagag ggtggccatc ctggactggg
     2161 acgtgcacca cggcaacggc acacaggaca tcttctacgg tgatggacg
//
LOCUS       DATMJA010000032         3152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_537930, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000032 DATMJA010000000
VERSION     DATMJA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537930"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VNT12_00735"
     CDS             complement(<1..1467)
                     /locus_tag="VNT12_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44662.1"
                     /translation="MSETKNYEVGSLRFAFTPQGDPFEGRLRAMAPELAPYIAEVKLA
                     SSASRNRYAKGAAEDCGMDQKELKRALNALCSLREEEVQAAREAERQRTAERSEEKPE
                     AGDEEIEAFVGRTGVLGRLVDEAARIHGVVGETAPLKLLALNALGAQLEPLPNGKPMG
                     ANVVLIAHWGRGKNHLCDAVAALLPEDFFLAFESASAMSLFYLAERNPRILSNRWIYL
                     NEAEAMDQLVELLRPLISGGKAVHLTVNKDSGGRNTAQELRIEGPVTTTIPTIRNKLD
                     AQLQSRMLLAELEDYKGRVASHTRKFNESLRPDFATENFSARIGAWQAALGSLTGIRR
                     VVFTLDREEFCFDRDDVPHGARLWANLIGMMFAHAWLEQRNREIIELPNGEKAIVATP
                     TDYAVAYGIFKAACERSVMNISDTHRRILDAIYQLQEEDKHIDHFRDKRWSQREIGRR
                     TGVPQSTISGQKSYLVRSLKLLDEDYNGKLRLAEDANPS"
     gene            complement(1464..2399)
                     /locus_tag="VNT12_00740"
     CDS             complement(1464..2399)
                     /locus_tag="VNT12_00740"
                     /inference="COORDINATES: protein motif:HMM:NF020291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HWI44663.1"
                     /translation="MVLDITTVNPRERIAGMFIEQLLYPLGANDRIEIRHKLPGDNKL
                     MRRRFFYDAVGAARYIIELDGEDVYVGAAPRYGSDGTKAGVLRTNALWTDLDFKDGHT
                     RESRLEQLRKLSCPPALGVATGGGLQHYWLLDRPAENPEGLERAELVMRRLAAAMGGD
                     SVHDRSRVMRVPETFNHKYGEPRPIRLEHYVPSWRYGLEELEAMTEAFAPETGSHAEQ
                     DGKVPREVLGDPIREGGRNVALTSVAGSLRNRGLDAETICVVLMEVNRLRCERPLADS
                     EVIGIIQSISRYPAGSARYLGSPARRVRREEGASR"
     gene            complement(2878..>3152)
                     /locus_tag="VNT12_00745"
     CDS             complement(2878..>3152)
                     /locus_tag="VNT12_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44664.1"
                     /translation="VAIDPLTTYPGQWSDPWEIMSADYFRRFAEEICPGLDRVFAEVV
                     WELGHSEDWDAPQNLRKVKRLSALRVEEVVKDAHARIQEWETTEGS"
BASE COUNT          505 a          997 c          961 g          689 t
ORIGIN      
        1 ggaggggttg gcgtcttcag cgagtcggag cttgccatta taatcctcgt cgaggagctt
       61 gaggctcctg accaggtagc ttttttggcc agagatcgtg ctctggggca ccccggtgcg
      121 cctccctatt tctcgctggg accacctttt atctcggaag tgatctatgt gtttatcctc
      181 ttcttgaagc tggtagatgg cgtcgagtat ccggcggtgg gtatcactaa tgttcatcac
      241 ggagcgctcg caggcggcct tgaagattcc gtaggctacc gcgtagtcgg tcggggttgc
      301 cacgatagct ttctcaccgt tgggtagctc gataatctcg cggttgcgct gctcgagcca
      361 ggcgtgggcg aacatcatcc cgatgaggtt tgcccagagc ctcgccccgt gcggcacgtc
      421 gtcgcggtca aagcagaact cttcacgatc gagggtgaag accacacgcc tgatgcccgt
      481 gaggctcccg agcgcggcct gccacgcccc tatgcgggcc gagaagttct cggtggcgaa
      541 gtctggcctc aaggattcgt tgaacttgcg cgtatggctg gcgacccggc ccttgtagtc
      601 ttcgagctcg gcaaggagca tgcggctctg aagctgggcg tcgagtttgt tacggatggt
      661 ggggatggtg gtcgtcactg gaccctcgat cctgagctcc tgggcggtgt tgcgcccgcc
      721 gctgtccttg ttgacggtga ggtgtacagc cttgccgcct gagatgagag gccggagcaa
      781 ctcaacgagc tggtccatag cctctgcctc gttcaggtag atccaacggt tcgacaggat
      841 cctggggttc ctctcggcca agtagaacaa ggacatggcg ctcgcgctct cgaaggccag
      901 gaagaagtcc tccggcagta gcgcggccac cgcatcgcac agatgattct ttccgcgtcc
      961 ccagtgggcg atgaggacga cgttggcgcc catgggcttg ccgttgggta gtggctcgag
     1021 ctgcgctccg agtgcattga gggcgaggag cttgagtggg gccgtctcac ctacgacccc
     1081 gtggatcctt gcggcctcgt cgacgaggcg acccaagaca ccggttctcc ctacgaaggc
     1141 ctctatctct tcgtcgcctg cctcgggttt ctcctcggat ctctcggcag tgcgctgacg
     1201 ctctgcctcc ctggccgcct ggacctcttc ctctctgagg gagcacaagg catttagcgc
     1261 cctcttcaac tccttctggt ccatcccgca gtcttcggcg gcccccttcg cgtagcggtt
     1321 gcgggaggcg ctgctggcca gcttcacttc agcgatgtaa ggcgcaagct caggagccat
     1381 tgcgcgcagg cggccttcga atgggtcgcc ctgcggcgtg aacgcgaacc ggagagatcc
     1441 gacctcgtag ttcttcgtct cgctcatctg ctggctccct cctcgcggcg aacgcgtcgg
     1501 gcgggcgagc ctaagtacct cgcggagccg gccggatagc ggctgatgga ctggatgatg
     1561 ccgatgacct ccgagtccgc aagcggccgc tcgcatctga gacggttgac ctccatcagg
     1621 acgacgcaga tcgtctccgc atcgagccct cggttccgca aggagcccgc aacgctcgtc
     1681 agggcgacgt tgcgcccgcc ctcccgtatc gggtcgccca ggacctcccg cggcaccttt
     1741 ccgtcttgtt ccgcatggct acctgtctcc ggggcgaagg cttcagtcat cgcctccagt
     1801 tcctcgaggc cgtaacgcca gctagggacg tagtgctcca ggcggatggg acggggctcg
     1861 ccgtacttgt ggttgaaggt ttccggtacc cgcatgaccc tagagcggtc gtggacggag
     1921 tcgccgccca tcgcagccgc caagcgtcgc atgacgagtt cggcccgctc cagcccctct
     1981 gggttctcgg cgggccggtc taaaagccag tagtgttgca gcccgccacc tgtcgcaacc
     2041 cctagggccg gcggacaaga gagcttccgg agctgctcca agcggctctc gcgggtgtgg
     2101 ccgtccttga agtccagatc cgtccacagc gcgttggtgc ggagcacgcc cgctttcgtg
     2161 ccgtcactgc cgtaacgagg cgccgcgcca acgtagacat cttcgccgtc gagctcgatg
     2221 atgtaccgcg cggctcccac ggcgtcataa aagaagcgcc gacgcataag cttgttgtcg
     2281 ccgggcaatt tgtgccggat ctctatgcga tcgttagcgc ctagagggta aaggagctgc
     2341 tcgataaaca tccccgctat ccgctcccgc gggttaacgg tggttatgtc tagtaccatt
     2401 gggtacgtcc tttccgagtc ccgttgtcat tgtgagaggt gctccgggaa ctctgggact
     2461 tagggccccg ccccgttcgc gcgggcgggg cctatcgttc attctcctac caagtctgct
     2521 acctcagttt ctaaagcctt tgctaacctc cttactgttc gtggatacgc tccccgccgc
     2581 ccggtctcga gctcccagat cgtgccctgg ccgatacccg cgagctcgct aagctcgcgt
     2641 tgcgtcaggc cggaccattg ccgggcctga cgcaagccgt gcaggaggac gcttctgttg
     2701 tggctactgg tcgctttcgc attcggggtc gccttcatag gttgtgctcc tagcctcagt
     2761 gatgccttct ctagactagg tgcgtaccag ggtcgaaagc gtcctcgaac actattatgc
     2821 ctggtatgca taacatcgat cggcggccta tcgcttctct tcgcgggggg atagctgtca
     2881 ggagccttcc gtcgtctccc actcctggat tcgtgcgtgc gcatctttga cgacttcttc
     2941 gacccgcagg gcgcttagcc tctttacctt gcggaggttt tggggagcgt cccagtcctc
     3001 cgagtgccct aactcccaga caacctccgc aaacaccctg tccaagccag ggcatatctc
     3061 ttcggcgaag cgacgaaagt aatcagcgct catgatctcc cacggatcag accactgccc
     3121 tgggtaggtg gtcaacgggt ctatggcgac ag
//
LOCUS       DATMJA010000033         5110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_479147, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000033 DATMJA010000000
VERSION     DATMJA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479147"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VNT12_00750"
     CDS             <1..274
                     /locus_tag="VNT12_00750"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWI44665.1"
                     /translation="EGSIGGLLLTVLIVAPVALQLLDFTIAKALVMGTGVSVSSQCGD
                     LFESSLKRILDVKDLGNFLPGHGGILDRVDSLLFTAPAVYYISLFV"
     gene            294..1433
                     /gene="dxr"
                     /locus_tag="VNT12_00755"
     CDS             294..1433
                     /gene="dxr"
                     /locus_tag="VNT12_00755"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWI44666.1"
                     /translation="MRRRLTILGSTGSIGSQALDVVRRYPERFEIVGLSAGNDARALA
                     LQAAEFGPEYLALEAGEAGPLEDLAAEVLAGPGSAEQLSRAPSDVVLNGIVGFAGLAA
                     TVGALQAGNRVALANKESLVAGGAWVMDLAAGNRIIPVDSEHSAIFQCLEGRRGEEIR
                     EILLTASGGPFFSLEKARLLEVGTEDALKHPTWRMGPKITIDSATMMNKGLEVIEAHH
                     LFGVALEDVRVVVHRQSVVHGGAFFEDGSVVLHAALPDMRLPIAVGLLYPERVDVGVE
                     PVPFDGVSWSFEEPRDAVFRCLPLAVEAGRAGGAHPVALNAANEVAVQAFLDGRIRFL
                     QIADVIEEVLEAVPDFGMMQSMEAIVAVDGWARGEAERRVGGDPR"
     gene            1430..2473
                     /gene="rseP"
                     /locus_tag="VNT12_00760"
     CDS             1430..2473
                     /gene="rseP"
                     /locus_tag="VNT12_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWI44667.1"
                     /translation="MTVIVALLGLIFLIVIHELGHMLTAKALGVRVPEFGVGFGPALV
                     KKKIGKTVYSFRIILLGGFAKMAGMGDGAEGRDTYYAKALWRRALIILAGPVANLLAA
                     VAILAGVYMFQGVVTDVKPVVSAVEQGSMAQEVGIQAGDRIVSLDGETVKTWDGFERQ
                     MSGRDVGEKVSVTVVRDGSQKTFEGELGADPEDKDRALVGVRPELETTTYGPVKSVWL
                     AIQRCGEITVRLGVFVGQLVTGEKDLYKNVTGPVGIASVGSTAVNQGFFLPLLALISL
                     NLALFNLFPVLPLDGGHLFFIAAEKVLGRPVSEQTMGKIAAFGLVLVLALFLFATYAD
                     LSKIFTGKPFIPD"
     gene            2505..3578
                     /gene="ispG"
                     /locus_tag="VNT12_00765"
                     /gene_synonym="gcpE"
     CDS             2505..3578
                     /gene="ispG"
                     /locus_tag="VNT12_00765"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWI44668.1"
                     /translation="MTEAALRERPVTSRQIRVGDVPIGGGAQVAVQSMTNTMTYDVEA
                     TVQQVYDLNAAGADLVRVSVNGSKALKGFREVVWRSPVPLIADIHFDYRMALGAADGG
                     AACVRINPGNVGSDDRYRQVIEKCQETGTAMRIGVNSGSVEKRFWNLPKAEALVASAL
                     HKVEIAEEMDFFDFKVSLKASHVPEMVEANKKFRVHSDAPLHLGVTEAGTKLPGAIKS
                     AAALGQLLPYGIGDTIRISLAEDPVEEIPVAYQILSALDLRHRGPNVIACPSCARTLG
                     FDVIGMAAKVEEGLKHFEDHFTVAVMGCVVNGPGEARDADYGVAGGKDDGVIFSKGQP
                     LRKVGRDEIYDALFEEIAKDDRK"
     gene            complement(3656..4111)
                     /locus_tag="VNT12_00770"
     CDS             complement(3656..4111)
                     /locus_tag="VNT12_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44669.1"
                     /translation="MTEASNTEFTTEELFSQAKGNATAFILTTIAYLKERGLDVNDFV
                     AFFGRQFAPGWDELRARQVAAVAQSVARNAISVGCALGSLSGDKARAEVLFTGWPDAQ
                     EISNVLGLEPYDRDAMWDSFYPIMERLGIRYAWQREDGTVRLIFERENE"
     gene            4542..4994
                     /locus_tag="VNT12_00775"
     CDS             4542..4994
                     /locus_tag="VNT12_00775"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44670.1"
                     /translation="MRETAELERLREVVEGVLPEGTEVVEARFSRGPLLTVLLDREDG
                     PVDHEFCSRVASVVAPAVEAEGYDGPIEVSSPGIERPLTRPEHFQRFVGHEAKVRIGE
                     PLDGRRNFTGVIDRAGEDAFVLRLSEGAEVELPFGSVTRANLKEDIYK"
BASE COUNT          961 a         1454 c         1680 g         1015 t
ORIGIN      
        1 ggagggcagc attggcggcc tcctcttgac ggtcctgatc gtcgcccccg tcgctctcca
       61 gctcctcgac tttaccatcg ccaaggccct cgtcatgggc accggtgtct ctgtctcgag
      121 ccagtgcggg gacctcttcg aatcgtcgtt gaagcgtatc ctcgatgtga aggacctcgg
      181 taacttcctc ccaggccacg gaggcatcct cgaccgggtg gacagcctgc tcttcacggc
      241 cccggcggta tactatatct ccctcttcgt atgaaaatgg acccaacacc gtcatgagac
      301 gccgcctcac gatcctcggc tcgacaggca gtatcggaag ccaagccctc gacgtcgtcc
      361 gcagatatcc tgaacgattt gaaatcgtcg gcctctcagc gggtaacgat gctcgggcgc
      421 tcgccctcca ggcggcggag ttcggacctg agtatctggc gctggaagcg ggcgaagcag
      481 gtcccctgga agatctcgcc gccgaagtcc tcgccggtcc cggctcggcc gagcaactct
      541 cccgcgcgcc gtcagacgtc gtgctgaacg ggatagtcgg cttcgcaggc ctcgctgcca
      601 ccgtcggcgc cctgcaggcg ggcaaccgcg tggccctcgc caacaaagaa tccctcgtcg
      661 caggaggcgc gtgggttatg gatctcgcgg caggtaaccg cataataccg gtagactccg
      721 agcactcggc gatcttccag tgtctggagg gcagacgggg tgaggagatc agggaaattc
      781 tgttgacggc gtcgggagga cctttcttct ccttggagaa ggcaaggctc ctcgaggttg
      841 ggactgagga tgcgctcaag caccccacgt ggcggatggg gccgaagatc actatagact
      901 cggccaccat gatgaacaag ggtctggagg tgattgaggc gcaccacctc ttcggcgtag
      961 cgttggagga cgtgcgcgtc gtcgtgcacc gccagtccgt ggtccatggg ggtgctttct
     1021 tcgaggatgg ctcggttgtg ttgcacgctg cgttgcctga catgaggctg cccatagccg
     1081 tcggcctgct ctatcccgag agagtcgacg tcggggtcga gcctgtaccc ttcgacggag
     1141 tcagctggag cttcgaggag ccgcgtgatg ctgtattcag gtgtctgccg ctcgctgtgg
     1201 aggccggacg tgccgggggt gcccatcccg tcgcgctcaa cgcggccaac gaggtcgctg
     1261 tgcaggcttt ccttgacggc aggataagat ttctacagat agcggatgtg atcgaggagg
     1321 tcctcgaggc ggtgccagac ttcgggatga tgcagagcat ggaagccata gtggccgtgg
     1381 acggttgggc gcgcggcgag gccgaaagac gcgtgggcgg agacccgcga tgaccgtcat
     1441 cgtcgcgctg ctgggtctga tcttcctcat agttatccac gagctcgggc acatgctcac
     1501 ggccaaggcc ctcggcgtaa gggttcccga gttcggcgtt ggcttcggac cagctctcgt
     1561 caagaagaag atcggcaaga ccgtctactc tttccgcata atactcctgg gtggatttgc
     1621 caagatggca gggatggggg atggggcgga ggggcgtgac acctactatg ccaaagcgtt
     1681 gtggcggagg gccctcatta ttcttgctgg cccagtggcc aacctcctcg cggctgtcgc
     1741 catactggcc ggtgtctaca tgtttcaggg tgttgtcacc gatgtgaagc cggtcgtgag
     1801 tgctgtcgag cagggttcga tggcgcagga ggttgggatc caggccggcg acaggattgt
     1861 gagcctggat ggcgaaacgg taaagacctg ggacggtttc gaacgccaga tgtcgggccg
     1921 ggacgttggt gagaaggtca gtgtgaccgt ggtgcgggac ggttcgcaga agaccttcga
     1981 gggggagttg ggggcagatc ccgaggataa agatcgggcg ctcgttggag tgcgtcccga
     2041 gttggagacc accacttacg ggccggtaaa gtccgtgtgg ctggcgattc agaggtgcgg
     2101 agagatcacg gtgcggctcg gggttttcgt cggccagctc gtaactggcg agaaggacct
     2161 gtacaagaac gtgaccggtc ctgtgggcat cgcctctgtc gggagcaccg ccgtcaacca
     2221 ggggttcttc ctgccgcttc tggccctcat cagccttaac ctggcattat tcaacctgtt
     2281 cccggtccta cctctggacg gcggtcacct gttctttata gcggcggaga aggtcttggg
     2341 caggcctgtc agcgagcaga cgatgggcaa gatcgccgct ttcggcctcg tcctcgtcct
     2401 ggccctattc cttttcgcga cctatgccga cctgagtaaa atcttcactg gaaagccgtt
     2461 cataccggat taagataggt gcgtaaagaa cgtatgggag tgagatgaca gaggcagcgc
     2521 tgcgggaacg cccggttacg agccggcaga taagggtagg cgacgtgcca atcggcggcg
     2581 gcgcccaggt ggccgtgcag tcgatgacga ataccatgac ctacgacgtc gaggcgaccg
     2641 tgcagcaggt ctacgatctc aacgcagcag gggctgacct ggtgcgcgtc tctgtcaacg
     2701 gctccaaggc gctgaaaggc ttcagggagg tcgtctggcg ttcaccggtg ccccttatcg
     2761 ccgacatcca cttcgattac aggatggcac tgggggctgc cgacggtggc gctgcgtgcg
     2821 tcaggatcaa cccgggcaac gtagggagcg acgaccgcta ccggcaggtg atcgagaaat
     2881 gccaggagac gggcacggcc atgcgcatcg gggtcaactc agggtccgta gagaagcggt
     2941 tctggaacct ccccaaggcc gaggcgctcg tggctagcgc cttgcacaag gtcgagatcg
     3001 ccgaagagat ggacttcttc gatttcaagg tctcgctcaa agcgagtcat gttccagaga
     3061 tggtggaggc caacaagaag ttccgcgtgc actctgatgc gccgctgcac ctcggtgtta
     3121 cagaggcggg gaccaagcta cccggtgcca taaagagtgc tgcggcgctt ggccagcttc
     3181 taccctacgg gatcggggac accatccgca tctcgctcgc cgaggacccc gttgaggaga
     3241 tacctgtggc ttaccagatc ctctcggccc tcgacctcag gcacaggggt ccgaacgtca
     3301 tcgcgtgtcc gagttgcgcc cgcacgctcg gattcgacgt gatcgggatg gcggcgaagg
     3361 tcgaggaggg gctcaagcac ttcgaggatc acttcaccgt cgccgtcatg ggatgcgtgg
     3421 tcaacggccc tggtgaggcc cgcgacgccg attacggcgt ggccggaggc aaagacgacg
     3481 gtgtgatctt ctccaagggt cagcccttgc ggaaagttgg ccgcgacgag atctacgacg
     3541 ccctcttcga agagatagcg aaggacgaca ggaagtaaat acggctttaa gtttcaggtt
     3601 ttgtcttgat ggttgggccg ttgacgagca attcacgacg cgaggacgcc cgttgctatt
     3661 cgttctcccg ttcgaagatc aacctcactg tcccgtcctc acgctgccag gcgtagcgga
     3721 ttcccaggcg ctccatgatg gggtagaagc tgtcccacat ggcgtcccta tcgtagggct
     3781 ccagtccgag gacgttcgaa atctcttgtg catccggcca ccccgtgaag agtacttcgg
     3841 cgcgtgcctt atctcccgag agcgagccca acgcgcagcc caccgagatc gcgttccggg
     3901 ccacgctctg cgcgacggcc gcgacctgcc gggcgcgtag ctcgtcccag ccgggtgcga
     3961 actggcgtcc gaagaaggcg acgaaatcat tcacgtccag gccgcgttcc ttcaagtagg
     4021 cgatcgtggt gagaatgaag gccgtcgcgt tgcccttggc ctgggaaaaa agctcctcgg
     4081 tcgtgaactc cgtgttcgag gcttcggtca tcgggactgc tcctctctgc cgcctgggga
     4141 atccggcaag tattgacgct tcccacatca accataatgc cagtgtgcgg gattctacgc
     4201 atccatggag agacttttcg ctcctgtacc tctggactca catacaagta cagcggatca
     4261 tgcccggcgt cgatgtagtc cagactaccg gccttggggt cgagcagggc gtagaagcag
     4321 gttagaaaat tgttggtaaa cgaaggcgcg ctgtgatctg aggcgatccc cacgttggta
     4381 gactccattg aaaggcttcg cggtcgacgc gagcggtgtc ctagacaatc ccctggcggc
     4441 cctgcgagcc ttgtaaccga catactctga gatataatct aatgtgctgc aagtgagcaa
     4501 ggggtcttcg ggcccgttgc tttttttatc gttcggagta ggtgagagag acggcggaat
     4561 tagagaggct gagagaggtg gtcgagggcg tgcttcccga gggaaccgag gtggtcgagg
     4621 ccaggttctc gaggggacct ttgctcaccg tgctcctgga ccgggaggac ggtcccgtgg
     4681 accacgagtt ctgttcgcgg gtcgcctccg tcgttgcccc ggcggtcgag gctgaaggct
     4741 acgatggtcc aatcgaggtc tcatcacctg gcatcgagcg tcccttgacg agacccgagc
     4801 actttcagcg tttcgtcggt catgaggcga aggtcagaat cggcgagccc ctggatgggc
     4861 gcaggaactt cacgggcgtc atagacaggg cgggcgaaga cgccttcgtg ctgagactct
     4921 ccgagggagc ggaggtggag ctgccgttcg gttccgtgac ccgcgcgaac ctcaaggagg
     4981 acatatacaa atgaatacgg cattgttatc cgcgctacac gagatagaga cggacaaagg
     5041 cattcccttc gagacggtga agggtgtgct cgaggagtcg ttgctcgcgg cttaccaggg
     5101 tcgcgagggg
//
LOCUS       DATMJA010000034         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_5216, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000034 DATMJA010000000
VERSION     DATMJA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5216"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..377
                     /locus_tag="VNT12_00780"
     CDS             180..377
                     /locus_tag="VNT12_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44671.1"
                     /translation="MEHRKSEAEVALELMRLVLEHAGSRRLSPTRTLALYHLCKEAVA
                     YSPEDRVTADYLEENLKKLLQ"
     gene            543..1469
                     /locus_tag="VNT12_00785"
     CDS             543..1469
                     /locus_tag="VNT12_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44672.1"
                     /translation="MSVLEDGSVETGLEGRGARCLAPDPKDPDTLYVGTSDEGIFKSE
                     DGGGTWEKLSGIEHPRITAVAVSPTDGAVYAGTEPSALFVSRDGGESWRELEGMRNLP
                     SAPTWSFPPRPWTSHVRAIALSHADPDLVVAGIELGGVVRSADGGETWQDQRPGAQAD
                     CHSLFAHPGAPELLYEAGGGGFAQSTDFGESWEAADGGMDLHYVWGLAADAEDPSLVY
                     TSAASGPGRAHGAGSSNATIYRRSGNEQWQPVLEGLFAFPYALCQDPETPGTLYAGFG
                     DGTILSTADAGARWEEMAHVASGTQDLASVSA"
     gene            complement(1552..1827)
                     /locus_tag="VNT12_00790"
     CDS             complement(1552..1827)
                     /locus_tag="VNT12_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI44673.1"
                     /translation="MDLPRLRRIRQGAVMSQEELAERSGVARDTISKLETGRRGAYPS
                     TIRKLAAGLEVRPQMLMGGVEYLDEPPQEEPSEKPSEKTEREKRIGF"
     gene            1930..2556
                     /locus_tag="VNT12_00795"
     CDS             1930..2556
                     /locus_tag="VNT12_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWI44674.1"
                     /translation="MSFDPRLVVPYVTEQSPRGERTMDIYSRLLKDRIVFLGTPVDDQ
                     VANVIMAQLLHLDAEDPEQDIHLYINSPGGSSSAMLAMYDTMQFVNADVATTALGMAA
                     SAGAFLLAAGAKGKRSALPNTRILLHQPSIQGLGGQASDVEIHAREIMDQKRRVNMIL
                     SERTGQDYEKIERDTDRDYILSSEAAVEYGVIDRVISRPDERVATVGK"
     gene            2586..>3276
                     /locus_tag="VNT12_00800"
     CDS             2586..>3276
                     /locus_tag="VNT12_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI44675.1"
                     /translation="MDLLILGGTGFLGRYLVEAALGDGHRPTLFNRGLHEPGLFPEVE
                     KIEGERDGDLSALRGQRWDAVIDTCGYVPRVVGTSAWLLADAVEHYTFVSSISVYSDA
                     IAPGGDEEAPVRELPDPTVEEVTEETYGGLKALCERAVEEMLPDRVLNVRPGLISGPH
                     DPTDRFTYWPRRVAAGGEILAPDHEERGVQYIDVRDLASWMVRMSEERRTGTYNATGP
                     DYELRMGRLLEE"
BASE COUNT          633 a         1013 c         1045 g          585 t
ORIGIN      
        1 gacgtaaagt ctgctcgact cacccacggg catccctaac ctctcgcggg ccgccgggcc
       61 gcgtttctga gtccctcttg gggcctggat ctgggatcaa agcgtccccg attctaccat
      121 agccgctcgc ttcgccggta taattccctg cacagaccgg cccggaggga ggcacccgta
      181 tggagcatcg caagagcgaa gccgaggtcg ccctggagct gatgcggctc gtcctggaac
      241 acgcaggaag taggcgactc tccccgaccc gtactctggc cctctaccac ctctgcaagg
      301 aggccgtcgc ctattccccg gaagaccgcg taaccgcaga ctacctagaa gaaaacctga
      361 agaagttgct gcaatagcat caacccgaaa ttatagccgt cagcctacca attcggccct
      421 tgcgtcaggt gttgcggagt tcatggctac ttgtgcggcg aggagtgtca tagcatactc
      481 tcaccggtac ggaaaaagga ggtctcgccg tggcacgatt gtatgtggca ggaggaagtg
      541 ccgtgtccgt gttagaggac ggtagcgtcg agaccgggct ggaagggcgc ggcgccaggt
      601 gcctggcgcc ggatccgaaa gatccggaca ctctctacgt cggcacctcg gatgagggta
      661 tcttcaagag cgaggacggc ggagggacgt gggagaagct ctccgggatc gaacacccca
      721 ggatcaccgc cgtcgccgtc tcgcccaccg atggggccgt ctacgcgggc accgaacctt
      781 cggccctgtt cgtaagccgt gatggcggtg agtcctggcg cgagctggag ggcatgagga
      841 atcttccctc cgcacctacc tggagcttcc cacccaggcc ctggacctcc cacgtccgcg
      901 cgatcgcgct ctcccatgca gacccggacc tcgtcgtggc cggcatagag cttggcgggg
      961 tggtgaggag cgccgacggc ggcgagacct ggcaggacca gaggcctgga gcccaggccg
     1021 actgccattc cctgttcgcc caccccggag cccccgaact cctgtacgag gccggcggcg
     1081 gcggattcgc ccagagcacg gacttcggcg agagctggga ggccgcagac ggggggatgg
     1141 atcttcacta cgtgtggggc ctggccgcgg acgcggaaga cccctcgctc gtctacacct
     1201 cagcagcctc ggggcccggc cgggcgcacg gcgcaggttc ctcgaatgcg accatctacc
     1261 gcaggtcagg taatgaacag tggcagccgg tcctcgaagg cctgttcgca ttcccctatg
     1321 ctctgtgcca ggacccggag acaccaggca cgttgtacgc cggcttcgga gacgggacga
     1381 tactcagcac cgcagatgcc ggagcgcgat gggaggagat ggcccatgtt gcctccggga
     1441 cccaggatct cgcgtcagtg tcagcctgaa gcagcatcct gttttggctg aaacctgtag
     1501 ccgcggttcg tatatgggcg gttcgctaac ggtccctaca agcacaccgc tctagaatcc
     1561 gatcctcttt tcgcgttcgg tcttctccga aggcttctcg gacggttcct cctggggcgg
     1621 ctcgtctaga tactctacgc cgcccatcag catctgtggc cttacttcca gaccggccgc
     1681 gagcttgcgg atcgtggatg ggtacgctcc cctccgcccc gtctcgagct tcgagatcgt
     1741 atcccgagcc accccactcc tctcggccaa ctcctcctga ctcatcacag ccccctgccg
     1801 aatgcgccgc aaacgcggaa ggtccatgct ccatacctcc aaaacactgc aaaaagcttg
     1861 cacaaaacct acaacaatgt tacgctagct ggagtatatc gtgttagcgg aagcagaggg
     1921 agtttggtga tgagttttga tcctaggctg gtcgttccct atgtgacgga gcagagtccg
     1981 cgtggggagc ggacgatgga tatctactcg cggctgctca aggacagaat agtcttcctt
     2041 ggcacgccgg tggacgatca ggtggcgaac gtaattatgg cgcagctctt gcatctggat
     2101 gctgaagacc cggagcagga catccatctg tacataaact cacctggtgg tagcagctcg
     2161 gcgatgctgg cgatgtacga caccatgcag ttcgtgaacg ccgacgtcgc tactaccgct
     2221 ttagggatgg cggcttcggc gggggcgttc ctgctagcgg caggggcgaa gggcaagagg
     2281 agcgcgctgc ccaacacgcg catcctgttg caccagccat ccatacaggg tctcggcggg
     2341 caggcctcgg acgtggagat acatgcccgg gagatcatgg accagaagcg acgcgtgaac
     2401 atgatactct ccgaacgcac cggccaggac tacgagaaga tagagcgcga caccgacagg
     2461 gactacatac tgagttccga ggctgctgta gagtacggcg tcatcgacag ggtcatcagc
     2521 cgtccggacg agcgcgtcgc cacggtcggc aagtaaggcc ccggaacctt cgaaagaagc
     2581 cgctgatgga cctacttata ctgggcggaa cgggattcct cgggcgctac ctcgtcgagg
     2641 cggcgctcgg ggacggccat cggcctacgc tcttcaaccg ggggctccac gagccagggc
     2701 tgtttcctga ggtcgaaaag atcgagggcg aaagggacgg ggacctctca gcccttcggg
     2761 ggcagcgctg ggacgcggtg atcgacacct gcggctacgt gccccgcgtc gtggggacgt
     2821 ctgcctggct acttgctgat gcggtcgaac attacacctt cgtctccagc atctccgttt
     2881 actctgacgc catcgcgccg gggggcgacg aagaggcgcc ggtgcgggag ctcccagacc
     2941 cgacggtcga ggaggttacg gaggagactt atggagggct caaggcactg tgcgagcggg
     3001 ccgtcgagga gatgctgccg gacagggtgt tgaacgtccg tccaggtttg atctctggtc
     3061 cgcacgaccc cacggaccgc tttacctact ggccccgccg ggtcgctgcc ggcggcgaaa
     3121 tacttgcccc tgaccacgaa gagcgtgggg tgcagtacat cgacgtgcga gacctcgcgt
     3181 cgtggatggt aaggatgtcg gaggaacgcc ggaccggaac gtataacgcc acgggccctg
     3241 actacgagtt acggatgggc cgattgctgg aagaat
//
LOCUS       DATMJA010000035         5342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_656608, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000035 DATMJA010000000
VERSION     DATMJA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656608"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VNT12_00805"
     CDS             complement(<1..237)
                     /locus_tag="VNT12_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI44676.1"
                     /translation="MERIGGENTGMPTSIRKVVVASFIGTTIEWYDYFIFATAAALFF
                     PDVFFPGFGDTTGTILAYTTFAVGFGARPLGGLIF"
     gene            549..686
                     /locus_tag="VNT12_00810"
     CDS             549..686
                     /locus_tag="VNT12_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44677.1"
                     /translation="MRYFADAIGRKRSTVIAVSGFGVATVQGGTVRLELILSEKVSAP
                     V"
     gene            683..1486
                     /locus_tag="VNT12_00815"
     CDS             683..1486
                     /locus_tag="VNT12_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWI44678.1"
                     /translation="MTDGARVFDLEQPRTEDMPIHPAHRQAGYSYHLHRRHEDEYRPE
                     EAGPRTGAAGIIVCGEHTGTHIDALCHQSDALTLFGDVPVDGSVQSPRGFHRLGVEEI
                     PPILAPGVLLDVAASKGADELEPGYAVTEQDLKYCCEEQGVNIEAGDVALVRTGNGCR
                     WEDEERYLAGAGMAASASYWLADRQVLAVGADNMAWDVPGLRDPELGCMMPGHLILLA
                     RRGIYIVENLVLEDLAASGHRRFDFICTPLKFVGATGSPVRPVAVIDGQ"
     gene            complement(1527..2711)
                     /locus_tag="VNT12_00820"
     CDS             complement(1527..2711)
                     /locus_tag="VNT12_00820"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI44679.1"
                     /translation="MLAATEGPGYTWRMENGRKLPLDGVRVLDLSRVLAGPYATMVLG
                     DLGADVLKVEHPERGDDTRHWGPPFAGGESAYFLSINRNKRSIGVDLKDPDGLERVEK
                     LAVGADVVIENWRRGALEKLGLGYEKVKRANPALIYCSITGFGPGPDEDRPGYDFLVQ
                     ARGGVMGITGQPGGEPTKVGVAIADIVCGLFASNAILAALHRRSVTGEGARIEVPLFE
                     STLGWLANRGQEYLTSGEETGLTGNAHPSIVPYQTFEASDKPLVVAVGNNTQFANLCE
                     IIGRTELAEDERFATNPDRVANREALISELQSAFGERPADEWADELRIAGVPSGPVNT
                     LADVLADEHLLGSGMLQDLDHPSAGHLKMLASPILIDGERLSIRRPPPTLGQHTEEGW
                     RR"
     gene            2799..4016
                     /locus_tag="VNT12_00825"
     CDS             2799..4016
                     /locus_tag="VNT12_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI44680.1"
                     /translation="MAVTKAGRTKDTGSSARPDPIDLLNIDAHLSDEERGGRERVRSF
                     VQERIKPNIEGWYDEAIFPREIVPEFAELGLLGMHLSGYGCAGKSAVEYGLACMELEA
                     GDSGLRTFVSVQGSLAMSAIHKFGSEDQKQRWLPPMARGETIGCFGLTEPTAGSDPAS
                     MKTFARRDGSDWVLNGEKRWIGLATIADVAVVWARTDEEKHPVRGFLVPTHTRGFSAT
                     DITPKLSMRASIQCDIRLQDVRLPEGAMLPEARGLSGPFACLGEARYGIIWGAMGAAR
                     DCYEAALAYAIEREQFGRPIASFQLVQQKLANMLIEVGKGTLLALHIGRMKDAGTLRP
                     EHISFGKLNNVREAIEIAREARTVFGGNGVTLDYPPMRHMSNLESVRTYEGTDEVHTL
                     ILGSAITGIPAFR"
     gene            complement(4057..4824)
                     /gene="fabG"
                     /locus_tag="VNT12_00830"
     CDS             complement(4057..4824)
                     /gene="fabG"
                     /locus_tag="VNT12_00830"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWI44681.1"
                     /translation="MRLDGRTAVVTGAARGIGAAEAIRLAQEGANLAVLDLSAEACQE
                     TVEAVEAAGSEAIAIACDVSSAQQVEKAFEEIAHRFGRIDILVNNAGLLRDNLSFKMS
                     EGDWDKVLDVHLKGSFLCSKAAQKYMVEQEYGKIVMTSSIVALGNKGQANYSAAKAGL
                     QALARTLALELGRFNINVNAVAPGWIETEMTKEAAERVGMTMEEMKDRFAKNIPLKRF
                     GRVDDIANVVAFLVSDDASYISGETIYVAGGPSSSVI"
     gene            complement(5068..>5342)
                     /locus_tag="VNT12_00835"
     CDS             complement(5068..>5342)
                     /locus_tag="VNT12_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI44682.1"
                     /translation="KAGLLGFTKTAAQELARFGITVNAIAPGFIETDMVANIPEEAQQ
                     KLRKQVPLGRFGKPEEVARAVRYLIEDGDYITGQSLDINGGIYIRT"
BASE COUNT         1027 a         1673 c         1667 g          975 t
ORIGIN      
        1 gaagataaga cctcccagag gacgcgcccc gaaccctacc gcgaaagtcg tgtaggcgag
       61 tatggtccct gtggtgtcac cgaagccggg aaagaagaca tcagggaaga agagagcagc
      121 tgccgttgcg aagataaagt agtcgtacca ctcgatggtc gtcccgataa aactcgctac
      181 aaccaccttg cggatcgaag taggcatccc cgtgttctcc ccgccgattc tttccatctt
      241 cgcctcctcg aaagagagaa tccctccgtc ccggagggat tataccggcc cgtcgtactc
      301 gccgctgcgg atgcgcgacc ttatgtcttg gacctcgtat ctacgggaac gcaggttctg
      361 ttccacagta ctcttctccc cttcgaccta ccttcatgct aaggatagct tgtgtcatgg
      421 ctggccggca agctcccgtc agcaggggat gatattcgaa cgacactggc gtatcatgtt
      481 ccatcgaatc tcggcctggg gaagcgcgag gaggaattac atggctacag cggcaggcgg
      541 aaggcgacat gaggtacttc gccgatgcca tagggaggaa acgctcgaca gtcatagccg
      601 tgagcggctt cggggttgcg acggttcaag gcgggacggt ccgccttgaa ctcatcttgt
      661 cagagaaagt gagcgcgccg gtatgacgga cggtgcgcgc gtcttcgacc tggagcaacc
      721 ccgcacggaa gacatgccca tccaccctgc ccatcggcag gccggctact cctaccactt
      781 gcaccgccgc cacgaggacg agtacaggcc tgaagaagca gggccgcgca ccggggctgc
      841 cggcatcatc gtctgcggag agcacacggg gacccacatc gacgcgcttt gccaccaatc
      901 ggatgctctg acgctcttcg gcgacgtccc cgtggacggg agcgtccaga gcccgcgcgg
      961 cttccacaga ctcggcgtcg aggagatacc cccgatactg gcccccgggg tcctcctcga
     1021 cgtggccgca agcaagggcg ccgacgagct cgagccaggc tacgccgtca cggagcaaga
     1081 tctgaagtac tgctgcgagg agcagggcgt aaacatcgag gccggcgacg tggcgctcgt
     1141 ccggaccggc aacggctgtc gctgggagga tgaagaacgc tacctcgcag gagcgggcat
     1201 ggctgcgagc gcctcgtact ggctcgccga caggcaggta ttggcggtcg gggcggacaa
     1261 catggcgtgg gacgtgcccg ggctcagaga tcccgagctg gggtgcatga tgccggggca
     1321 tctgatcctc ctggcccgcc gcggcatcta catcgtcgag aatctggtac tggaggatct
     1381 cgccgcctcg ggacaccgtc gcttcgactt catctgcacg cccttgaagt tcgtcggggc
     1441 gacaggttct cctgtcaggc ccgtggcggt aatcgacggc caataagaaa ggggtgcagg
     1501 attcgccctt tcagcgcccg gtcagactat cgccgccaac cttcttcggt gtgctgcccg
     1561 agggtcggtg gggggcggcg gatggacagg cgttcgccgt cgatgaggat cggcgaggcg
     1621 agcattttga ggtgtccggc cgagggatga tccaggtcct gtagcatgcc cgagccgagg
     1681 aggtgctcgt cggccaacac gtcagccagt gtgttgactg gaccactggg gacaccggcg
     1741 atccgaagct catcggccca ctcgtcagcg ggccgctcgc cgaatgcact ctgaagctct
     1801 gagatcaaag cctccctgtt ggccacgcgg tcagggttgg tcgcgaaccg ttcatcctcg
     1861 gccagctcgg tacggccgat gatctcgcac aggttggcga actgggtgtt gttacctacg
     1921 gctacgacaa gaggtttgtc tgaggcttcg aaggtctggt atggaacgat ggaggggtgc
     1981 gcgttacctg taagtccggt ttcctcgccg ctcgtcaggt actcctggcc gcggttggcg
     2041 agccagccga gtgtagactc gaaaagaggc acttcgatgc gtgcgccctc tcctgtcacg
     2101 ctgcgccggt gcagggcggc caggatcgcg ttcgacgcga aaaggccgca cactatatca
     2161 gcgatcgcaa ccccgacctt cgtcggttcg ccgccaggct gacccgtgat gcccatgaca
     2221 ccgcccctgg cctgcaccag aaagtcgtag ccaggacggt cctcgtcagg cccgggaccg
     2281 aagcccgtga tggagcagta gatcagggct ggattcgctc ttttgacctt ctcgtaaccc
     2341 aggccgagct tctccagggc tccgcgcctc cagttttcga tcacgacgtc agccccgacc
     2401 gccagcttct cgacccgctc gagcccatca gggtctttca gatcgacccc gatcgaacgc
     2461 ttgttgcggt tgatggagag aaaataggcc gactcgccgc cggcgaaggg cgggccccag
     2521 tggcgggtgt cgtccccgcg ttcggggtgc tcgaccttca acacgtcggc gcccaggtca
     2581 ccgagcacca tcgtcgcgta aggcccggcc aggacccgcg agagatcgag gaccctgacg
     2641 ccgtctagcg gcagcttccg accattctcc atgcgccaag tgtagcccgg tccctccgta
     2701 gcggccagca cgcagcatca gagaaggtgt tcgcaacgcg gagatgtgcg ctgtggaata
     2761 atccgggttg atggtgaggc tccgagagag gtgagaagat ggctgtgacc aaggccggaa
     2821 gaaccaagga caccggctcc tcggcccgcc ccgatccgat agatctcctg aacatcgacg
     2881 cccacctctc cgatgaggag agaggcggaa gggagagggt cagatctttt gtgcaagaga
     2941 ggatcaaacc gaacatcgaa gggtggtacg acgaggccat cttccccagg gagatagtac
     3001 ccgagttcgc ggagctcggc ctgctcggga tgcacctctc cggctacggg tgcgcgggca
     3061 agagcgcggt cgagtacggc ctggcgtgca tggagctcga ggccggcgac tccggcctga
     3121 gaaccttcgt cagcgtacag ggctcactcg ccatgagcgc catccacaag ttcgggtcag
     3181 aggaccagaa acagaggtgg ctgccaccga tggcgagagg agagactatc ggctgtttcg
     3241 gcctcaccga gccgaccgcc ggctcagacc cggcgagcat gaagaccttc gccaggaggg
     3301 acggctccga ctgggtcttg aacggggaga agcgttggat cgggcttgcc accatcgccg
     3361 acgtcgccgt cgtctgggcg cgtaccgatg aagagaagca ccccgtccga ggcttcctcg
     3421 tcccaacaca tacgcgaggg ttctcggcca cagacataac ccccaaactc tcgatgcggg
     3481 cttcgatcca gtgcgacata aggctccaag acgtccgcct ccccgagggc gcgatgctac
     3541 ccgaggccag gggcctcagc ggaccattcg cctgcctggg cgaagccagg tacggcatca
     3601 tctggggcgc gatgggggcg gcgcgagact gctacgaggc cgcactggct tacgctatag
     3661 agcgggagca gttcggacgg cccattgcat ctttccagct cgtccagcag aagctcgcga
     3721 acatgctgat cgaggtcggt aagggtacgc tcctcgcgct tcacataggc aggatgaagg
     3781 acgcaggcac gctcaggccc gagcacataa gcttcggcaa gctaaacaac gtcagggagg
     3841 ccatagagat agcccgcgaa gcccgcaccg tcttcggcgg caacggcgtc acgctcgact
     3901 atcctccaat gcgccacatg agcaacctgg agagcgtgag gacctacgag ggcacggacg
     3961 aggtacacac cctgatcctc ggcagcgcca tcacaggtat ccccgcattc cgttagagca
     4021 tttcagcaag tcagcacggc gccgtgctga aatgctctaa atcactgaac tcgagggacc
     4081 gccggcaacg taaatcgtct caccactgat gtacgaggcg tcgtcggaga cgaggaaggc
     4141 gacgacgttg gcaatgtcgt ccaccctgcc gaagcgcttg agggggatgt tcttggcgaa
     4201 gcggtccttc atctcctcca tagtcatccc gacccgctcg gccgcctcct tggtcatctc
     4261 ggtctcgatc caacccggag ccaccgcgtt gacgttgatg ttgaaacgcc cgagctcgag
     4321 cgccagcgtc cgcgcgagcg cctggagacc agccttggcc gccgagtagt tggcctgacc
     4381 cttgttcccc agagccacga tggagctggt catcacgatc ttgccgtact cctgctcgac
     4441 catgtacttc tgcgcagcct tggagcagag gaagctgccc ttgagatgca cgtcgagcac
     4501 cttgtcccag tcgccctcgc tcatcttgaa cgagaggttg tcgcgcaaca gcccggcgtt
     4561 gttgacgaga atgtcgatcc gcccaaagcg gtgagctatc tcctcgaagg ccttctcgac
     4621 ctgctgcgca ctcgatacgt cgcaggcgat cgcaatcgcc tctgaccccg cagcctcgac
     4681 ggcctccacc gtttcctgac aggcctccgc cgatagatcc aggaccgcta ggttcgcccc
     4741 ctcctgcgcc aaccgaatgg cctcagccgc cccaatgccg cgcgcagccc ccgttacaac
     4801 cgccgtacgt ccgtcaagcc tcaagatcca cccctcggtt cgggaacacc gccgtctaca
     4861 cccaaggagg gtgtcgtctt tgttacatga cagttaattg tatacgaggc gaacttatgt
     4921 gtccagaaca gatatataca tctttggatg caaaatctgg ctctctggcg ggatcgtatg
     4981 cgtttggggg tgggctgcct ttctgcagga tcacccggcc gggtgtggga gggagcaccg
     5041 cccgcgggcg ggcggtgctc tccctcgtta cgtcctgatg tagataccgc cgtttatatc
     5101 gagtgactgg cctgtgatgt agtcaccgtc ttcgataagg tagcggacgg ccctggctac
     5161 ctcttccggt tttccgaagc ggcccagggg cacttgtttg cggagcttct gttgcgcttc
     5221 ctcggggatg ttggcgacca tatcggtctc tatgaatcct ggggctatgg cgttgacggt
     5281 gatcccgaag cgggccagtt cctgggctgc agtcttggtg aacccgagta gcccggcctt
     5341 gg
//
LOCUS       DATMJA010000036         7573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_716492, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000036 DATMJA010000000
VERSION     DATMJA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716492"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VNT12_00840"
     CDS             <1..339
                     /locus_tag="VNT12_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44683.1"
                     /translation="CDRVTIIRDGKAGESGTLSDLRHLSRTSISAELARTPVGLAGLP
                     GVHDLRVEGNRVRCQVDASGIDGLLTHLTPFGVRSLISQPPTLEDLFLRHYETEERDA
                     QPIDRAGVAR"
     gene            336..1934
                     /locus_tag="VNT12_00845"
     CDS             336..1934
                     /locus_tag="VNT12_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44684.1"
                     /translation="MKALAGTWSLVRLILRRDRIILPLWVAVLALLPVAIANTLADLY
                     PTAAERQSYLDIVTANPALSALYGHAYGSGLGALTAWRLGGTVLLVGLATLLTVIRHT
                     RAEEVAGRRELLGATVVGRQAPLFAALVVAFGASLALGALVSVGLVAYGLPTTGSVAF
                     GLSWAMFGWAFAAIAAVAAQATESARVARGISITVLGLCFVLRAAGDAGNEDGPSWLS
                     WLSPFGWAQRIRPFVDERWWVFLLFLGLIIVLTFAALALSARRDLGSGLLPTRLGPAV
                     ASPWLRSPLALAWRLQRGTLLAWTAGFVVFGAILGNVGDSAVEVIEDNPQLKAVAEQL
                     GGSSGLSDAYFAVVMGLLGLVASGYAVGAALRLRSEEANGRAEPVLGAAVSRARWAAS
                     HLTFALLGPAVVLGAAGLAAGLAYGLSAGDVGQEMARVLGAALVQLPAVWVLVGVAVA
                     LFGVLPRFMAFGGWAALAACALIEEFGRPLQLGKRVLALSPFAHVPRLPGGDVSAAPL
                     LWLVLIVVALAAVGILGFRRRDVL"
     gene            complement(1954..2907)
                     /locus_tag="VNT12_00850"
     CDS             complement(1954..2907)
                     /locus_tag="VNT12_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HWI44685.1"
                     /translation="MSRTARLLELLIRVQTKPRFTAAELAEEFGVSRRTMLRDLSALS
                     AMGVPLRSTPGPGGGYSLPRGGRRLSPSLTVDEALALIASYEALLRSYPVHPFSTQSL
                     SAVTKLRAALPKDVVAQLDRLRRHVFVGGPVREYEAPLLGELLSAALDRAHLKVTYDS
                     IESGVTERVIFPYGLYASQGYWYCACFDRRRETNVPMRADRFLSAERVEGFEPPPELS
                     IQGWMGAARGVGERVRVMARVTERGRKSFELTSLFGSIEAQKGGGGALEAEIPRSEID
                     YYASRLLSVGTEIFVDSPPELVEAIQNKAREVIRLYGSRSS"
     gene            3013..3375
                     /locus_tag="VNT12_00855"
     CDS             3013..3375
                     /locus_tag="VNT12_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI44686.1"
                     /translation="MSNPIVHVEVVGEDAEALQGFYRDAFDWQMRPSGPDYAMAHPGV
                     EGGIDGGVGASPEGGQGHVTFYVEVGDLEATLGKIEGLGGSRVTGPMDVPDGPRIAMF
                     ADPEDHVVGLIEAGSRRS"
     gene            3870..4730
                     /locus_tag="VNT12_00860"
     CDS             3870..4730
                     /locus_tag="VNT12_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWI44687.1"
                     /translation="MQLVSGDGRQHLNRVVDSTHQHLLICSPFITTVEMSRVIEKLRA
                     NVNFNAMSVEVMTDLRPDSVLAGSLHVQALLDLLDAGCQAKITALARIHAKVYLADTS
                     VAWITSANLTTAGLEHNLEYGVLINDPSVAANILRDMREYAQLGTVATRLQVQNYLTA
                     TEALRKDYETFRRTSERMFRQQFRERLEEASIEAIRLQIGNRSAHAVFADAIVFLLHS
                     GPMTTTEMHPKIQVLLPELCDDRRDRVIDGRHFGKLWKHHVRTAQQHLKRTGVIDYQN
                     GIWKLQGLTR"
     gene            4851..5993
                     /locus_tag="VNT12_00865"
     CDS             4851..5993
                     /locus_tag="VNT12_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44688.1"
                     /translation="MIDPQSQTKMRQEINSRIENDYELLKNLREEIRPLKSGVRRIQP
                     RTTTSISLVGTDGGNNRIQFDPFLLQLVRVVDTSNNEYCLEAVTPTTDVSCLSESQFD
                     EHGAAASPLGRMMEYLGTEYLWELSPMIRRNDDERPVSPSWVQVYRELVEWAILLSLV
                     RDKDFGTDTLIIFDGLLRSKVFTGEMFADYRRGLAEAVDAQWRENRRRVYLAGVAKHS
                     KVLSRYKLAMSLESVLTTDYPAYVEVPPDIEEKAYVWSEYARGDDQAAEGGEANKFVG
                     GKMFFVKFGPGPRDPIWPVDIFLPQARDAQQIVGYMLADAVNGFPVPFYPQCLQKAHE
                     NAALVDFDFDILQDQVFDGLRAVLGEDAQALDTFRLQNGDVAQRRY"
     gene            6013..6645
                     /locus_tag="VNT12_00870"
     CDS             6013..6645
                     /locus_tag="VNT12_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44689.1"
                     /translation="MELFPSEQIVGIFRGFREGGMEFHADLVLPYRNDFQSIPMHGQF
                     LLVQLETPEEAVLGRIASFSSDGKLSFGSGEEFNIRAVREGRAVPEDLREQYLKYRVN
                     IRVLGVLRNGADGKPRFVASHRRLPHVGSAVALPSSDVLREIAGHNEEGAELGHFALG
                     EYVYASGSDKLALEDWMQTKEPEILVKFPVGSLATLPSLVTPSGTGKSTT"
BASE COUNT         1475 a         2337 c         2314 g         1447 t
ORIGIN      
        1 tgcgaccggg tcaccataat cagggacgga aaggcgggag agagtggcac gctctccgat
       61 ctcaggcatc tgagcaggac ctcgatctcg gccgagttgg ccaggacgcc cgtggggctt
      121 gccgggctac cgggcgtcca cgatcttcgc gtcgagggta accgggtgcg ctgccaggtg
      181 gacgcctccg ggatcgatgg cctgctcacg cacctgacgc ctttcggggt gcggagcctg
      241 atcagccagc ctcccaccct cgaagacctg ttcctcagac actacgagac cgaagagcgg
      301 gacgcacagc cgatcgaccg ggctggagtg gcgcgatgaa ggcgcttgcg ggaacctggt
      361 cgctcgtgcg gctcatactg cgccgtgacc ggatcatcct gccgctctgg gtcgcggtgc
      421 tggccctcct cccggtcgcg atcgcaaaca ccctcgccga cctctaccct accgcggcgg
      481 agcgccagtc gtacctggac atcgtcaccg caaaccctgc cctgagcgcg ctgtacggcc
      541 acgcgtacgg ttccggcctc ggcgctttga cggcgtggcg gctgggcggc acggtcctgc
      601 tggtcgggct ggcgaccctg ctcaccgtca tccgtcacac ccgagccgaa gaagtagcag
      661 gaaggcgcga actcctgggc gcgaccgtcg taggccggca ggcgcccctc ttcgcggccc
      721 tcgtcgtggc tttcggcgcg agtctggctc tgggcgccct cgtctcggtc gggttggtgg
      781 cctacgggct ccccaccaca gggtccgtgg cgttcgggct ctcgtgggcc atgttcgggt
      841 gggcattcgc cgccatcgcg gccgtggccg cgcaggcgac cgaaagcgcg cgggtcgcaa
      901 ggggcatctc catcaccgtc ctcggcctgt gcttcgttct gcgagccgcg ggcgacgcgg
      961 gcaacgaaga cggtccatcg tggctgtcct ggctctcgcc gttcggctgg gcccagcgca
     1021 tcaggccctt cgtcgacgag cgctggtggg tctttctgct cttcctcggg ctcattatcg
     1081 tgctgacctt cgccgccctc gcactctcgg cgcggcggga tctcgggagc ggactcctgc
     1141 cgacacggct cgggcccgcc gtcgcgtcac cctggttgcg cagcccgctc gccctggcct
     1201 ggcgattgca gcggggcacg ctccttgcct ggaccgccgg gttcgtcgtg ttcggtgcca
     1261 tactcggcaa cgtaggcgac agtgccgtgg aggtgatcga agacaacccg cagctcaagg
     1321 ccgtggccga gcagttgggc ggctcctccg gactctctga tgcctatttc gccgtggtca
     1381 tgggcctcct cggtctcgtg gcctcggggt acgccgtagg tgccgcgctc aggctgagaa
     1441 gcgaggaggc aaacgggcgt gccgagcccg tgctcggggc cgcggtctcc cgggcgcggt
     1501 gggccgcgag ccacctgacc ttcgcgcttc tgggaccggc ggtcgttctg ggagcggccg
     1561 gcctcgccgc agggcttgcc tacgggctga gcgccggcga cgtgggccag gagatggcgc
     1621 gcgtgctggg ggccgctctc gtacaacttc ccgccgtctg ggtgctggtc ggggtggcgg
     1681 ttgccctgtt cggggtgctg cctcggttca tggcgttcgg gggctgggct gccctggcgg
     1741 cgtgcgcgct gatcgaggag ttcggccggc cgctgcagct aggcaagcgg gtgctcgccc
     1801 tctcgccctt cgcccacgtg ccgaggctac cgggcggaga cgtctcagct gcgccgctcc
     1861 tgtggctggt gctgatcgtc gttgccctcg ccgcggtcgg aatcctgggc ttccggagac
     1921 gcgatgtcct ctgatccaac ctcgaggaag gcttcaggaa gaccgggacc catagagacg
     1981 aatgacctct cgggccttgt tttggatggc ctcgaccaac tccggcgggg aatccacgaa
     2041 gatttccgtg ccgacgctca gcaggcgcga ggcgtagtag tcgatctccg agcgtggaat
     2101 ctccgcctcg agagcgccgc caccaccctt ttgggcttcg atgctgccga agagggatgt
     2161 aagttcgaag ctcttcctcc cccgctcggt aacccgggcc atcacccgga ccctctcgcc
     2221 gaccccccgc gctgcgccca tccagccttg gatagagagt tcgggcggtg gctcgaagcc
     2281 ctctaccctc tcggccgaga gaaagcggtc cgccctcatc gggacgttcg tctcccttct
     2341 tcggtcgaag caggcgcagt accagtaccc ctgcgaggcg tacaggccgt aggggaagat
     2401 cacccgttcg gtgacccccg actcgatgga atcgtaggta accttcaggt gtgctctgtc
     2461 gagcgccgcc gacagtagct cgcccaagag cggggcctcg tactcgcgca ccggcccacc
     2521 cacgaagacg tgcctcctca gcctgtcgag ctgtgccacg acgtccttgg gcaaggcggc
     2581 cctgagcttg gtcaccgctg agaggctctg cgtagagaag gggtgtaccg ggtagctcct
     2641 gagcagggct tcgtaggagg cgatcaaggc gagcgcctca tccacggtca aggacggtga
     2701 gagcctcctt ccgccccgcg gcagggagta cccgccccca ggacccggcg tagagcgtaa
     2761 aggcacgccc atagccgaaa gcgcgctcag gtccctgagc atcgtgcgcc tgctgactcc
     2821 gaactcctcg gccagctcgg cggccgtgaa gcggggcttg gtctgaaccc ttatgagtaa
     2881 ctccaacaac ctcgcggtgc gcgacaaatc tatcctccga aacttcgagg taggtgacgc
     2941 tcgttgtcac cattatcgat tagatttcat ccaaccgcac cggtagagcg gcagaacaga
     3001 gaggagaagt tgatgagtaa cccgatagtg cacgtcgagg tcgtcggcga ggacgccgag
     3061 gccctccagg gcttctacag ggacgccttt gactggcaga tgcggccttc gggcccggac
     3121 tacgcgatgg cgcaccccgg cgtagagggc ggcatcgacg gcggcgtcgg cgcctccccc
     3181 gaaggaggcc agggccacgt caccttctac gtggaggtcg gggacctcga ggcgacgctg
     3241 ggaaagatcg agggcctcgg tgggagcagg gtgacggggc ccatggacgt gccggacggc
     3301 ccgcgcatcg cgatgttcgc cgaccccgag gaccatgttg tgggcctcat cgaggccgga
     3361 tcccgacgca gctgaagcaa ggtacctaac ggaaggaggt tggttccgcc cgggagaaga
     3421 gcgaaggtaa gcacgaaagt attggtaagg gacgcctcgg cttctacgcc aaccagtatc
     3481 cgcaatcagc aaccgggaac cctcacgccg acttcttacc tgccctcgcc agatccagca
     3541 cgccctcatc aacaacggac gctgcgacta cctctcatgg tcctcggccc aggagtacct
     3601 gagggcgctc ctgggacgcc cagctgctct acctggacgg ctccgggcac aacgcttacc
     3661 aggacgagcc gaagcgttac atggcggagg tgcgttcttg cgagatcgag cccttccgta
     3721 ccagggcacc ggtccacctg atgattacga agggccaccc tgaacccgtt tctccgtata
     3781 cgacggctca tggcggatct tcttgggagt ccgtttgtac tcgacgcctt acggagaact
     3841 gttgacatta gggatagaat tttggtccta tgcaacttgt ttctggggat ggtcggcagc
     3901 atctaaatag agtcgtagac tcaacgcatc aacacttact gatttgctcg cctttcatta
     3961 ccactgtaga gatgagtcgc gtaatcgaaa agctacgagc aaatgtaaac ttcaatgcga
     4021 tgtccgtaga agtcatgacc gacttgagac ctgacagtgt gctggcagga tcgcttcacg
     4081 tgcaagctct actcgatcta ctcgacgcag gctgtcaggc caagataacg gctctggcac
     4141 gaatccacgc taaggtttac ttggctgaca cgagtgtagc ctggataacc tcagccaatt
     4201 taactactgc cggccttgag cataatctcg aatatggtgt tctcatcaac gatccttcgg
     4261 tggcagcaaa tattttgcgg gacatgaggg agtacgctca actaggtacg gtagcgacac
     4321 gtctccaagt tcagaattat ctgactgcaa cagaagctct acgtaaggat tatgagacgt
     4381 ttcggaggac ttccgagaga atgtttcggc agcagtttcg agaacgatta gaagaagcat
     4441 ctatagaagc tattcgccta caaataggca accgttctgc gcatgccgtg ttcgctgacg
     4501 caattgtctt tttgctgcat tctggtccta tgactacgac agagatgcac cccaaaatac
     4561 aggttctatt gcctgagttg tgtgatgatc gacgtgatcg agtgatcgac ggtaggcatt
     4621 tcggcaagct ctggaaacat catgtgcgta cagcgcaaca acacctcaag cgaacaggag
     4681 taattgatta ccaaaacggt atttggaaac tccagggact cacaaggtaa ctcagaaccc
     4741 tagcccacaa taatctcgac ctgagccacc cgtatcaaca taagtgtatc gattattgcc
     4801 ttcagaaggt atcattagcc ctcgaggatt cgatgcaagg agcaatttag gtgatagatc
     4861 cgcaaagtca gacgaagatg cggcaagaga taaactctcg cattgagaat gactacgaac
     4921 tcttgaagaa ccttagggaa gagatcagac ctctaaaaag cggcgtccgg cgcatccagc
     4981 cgagaaccac cacctctata tctttggtcg gaacagacgg tggcaacaac cggatccagt
     5041 tcgatccctt ccttctacag ctcgtcaggg tagtggacac cagcaacaac gaatattgtc
     5101 tcgaagccgt caccccgacc accgacgttt cctgcttgag tgaaagccag ttcgacgagc
     5161 atggcgctgc ggcatccccg ctgggtcgga tgatggaata cctgggtacg gaatatcttt
     5221 gggagctcag tcccatgata cggcgcaatg acgacgagcg tccggtgagc ccgagctggg
     5281 tacaggtcta cagggagtta gtcgagtggg ccatcctgct ctccctcgtc agagacaaag
     5341 acttcggcac tgataccttg atcatcttcg acggcctgct caggagcaaa gtcttcacgg
     5401 gcgaaatgtt cgcagattac cgtagaggcc tcgctgaggc cgtggacgcc cagtggcgcg
     5461 agaaccggcg ccgggtgtac ctcgctggcg tcgccaagca cagcaaagta ctctctcgtt
     5521 acaaacttgc aatgtcgctt gagagcgtac tcaccaccga ctacccggca tacgtcgagg
     5581 tacccccgga catagaagag aaggcctacg tgtggtcgga atacgcgcgt ggggacgacc
     5641 aggctgccga aggcggggag gccaacaagt ttgtcggggg caagatgttt ttcgtcaagt
     5701 tcggccccgg accgcgggat ccaatctggc cggtggacat cttcctcccg caggcccgcg
     5761 acgcacagca aattgtgggc tacatgctcg ccgacgccgt caacggattc ccggtgccgt
     5821 tctaccctca gtgtctccag aaggcgcacg agaatgcggc tctcgtggac ttcgacttcg
     5881 acatcctcca ggaccaggtg ttcgacgggc tgcgcgcggt cctgggcgag gacgcgcaag
     5941 ccctggacac cttccgcctg caaaacggcg acgtggccca gcggagatac taggagaaag
     6001 gatatacagg acgtggagct tttcccgagc gagcagatag taggcatctt ccgcggtttc
     6061 cgcgaaggcg ggatggagtt ccacgccgac ctcgttctac cctaccgaaa tgacttccag
     6121 agcatcccca tgcacgggca gttcctactg gtgcaactcg agactcccga ggaggctgtg
     6181 ctgggcagaa tcgcttcatt ctcatccgac ggcaagctct cattcggctc cggggaagag
     6241 ttcaacatac gcgccgtccg ggagggcaga gcggtcccgg aggacctgcg tgagcagtac
     6301 ctcaagtacc gggtgaacat cagagtcctc ggcgtcctta gaaacggcgc cgacggcaag
     6361 ccgcggttcg tcgcctccca tcgacgcttg ccgcacgtcg ggagcgcggt ggccctgcct
     6421 tcgagcgacg tcctgcgcga gatagccggc cacaacgagg aaggcgctga gctaggccac
     6481 ttcgccctcg gcgagtacgt gtacgcgagc ggctcagaca agcttgccct cgaggactgg
     6541 atgcagacga aggagcccga gatcctggtc aaattccccg tcggcagcct cgctacgctc
     6601 ccaagcctcg ttacgccgtc gggtactgga aaaagcacaa cctgatcccc gagccggttg
     6661 acgtactgga gatagtttgc gagcgtgccg aacgctacta ctccaacgag gcgactgctt
     6721 ccggcaagat catccgtggc gatagtcgca agaacgtctt cgcgtgggct actagagggc
     6781 agaaggccga ctggatcatc acctcgccac cctactacgg gctacgcacc tacctgcccg
     6841 accaatggct gagacattgg ttcttaggcg gtcctgcgaa agtcgagtac tccgccgagg
     6901 gccagatact ccattcgagc ccgaaggcct tcgcctcgga tctaagacga gtgtggcgta
     6961 acgtcggcaa gatcagtgcg ccagatgcgc agctggtggt caggttcgga ggcatcaacg
     7021 acagaaaggc cgacccgatc tccataatca atgtgtcctt agaaggctcc ggcttcgagc
     7081 tccagacgat aacgcctgct ggatcggcct ccagcggcca gcgtcaagcc ctgcacttct
     7141 cgcgaccgag tgatgatgca aggcaggagc atgacatttg ggctaccaag atcacgtaaa
     7201 ccacgaactc ctaggagctg cctaggcttt ctcgtcctat caaggccgct gctcacccat
     7261 aatgttgaga tttcctacgg ccaaatcgtg gccgagaaca tggccgtccg tgtagttcct
     7321 taccataaca cctttatgct gactaaagct tacggagtcg ccttggcata gggtccacac
     7381 tcgtagccac agccgaccaa tacatttacc ctgactgccg ccctcaggtt gtcgaggcgt
     7441 tcgacgccat ggagcgcagg gctgtcgaga gattcggaga tccggaatta ggtctgcaca
     7501 ctccctggac actagcgtcg cgatcgagtg gagcctcatt cctttttgac cttgcttagt
     7561 atgacattac cgt
//
LOCUS       DATMJA010000037         3037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_420243, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000037 DATMJA010000000
VERSION     DATMJA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420243"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /gene="serA"
                     /locus_tag="VNT12_00875"
     CDS             complement(<1..1281)
                     /gene="serA"
                     /locus_tag="VNT12_00875"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWI44690.1"
                     /translation="MRVLVPERLADPGIELLEQDFEVDVLLDLRPEELLQKIGDYDGL
                     IIRSATKVTAEVIERAGNLKAIGRAGIGVDNIDIEAATKRGIIVANAPESNTVAAAEH
                     TLGLMLAVARRIPAADSSLRGGEWKRSALKGVEVSGKTIGLVGLGHVGAIVARGALGM
                     GMQVLAYDPYVSEDRMHSMNVERAETADEVFERADFVSLHVPRTPQTMGLVSEDALAK
                     MKPTAYLINVARGGIVDETALYNALKEGAIAGAALDVFAEEPATDSPIFTLSNVVVTP
                     HLGASTAEAQDRAGITAAEQVAAALSGAVPVHAINAPVPMGEGAEFVAQFSGLCESLG
                     SLLYQLTDRTDDTLRIEYRGEVAGFDTRLLDVSAQKGLLAPMVFEPLNFVNTPALAKE
                     RGLKVETSRSSESADYTNLVELRMGENAVSGTLFG"
     gene            complement(1278..2435)
                     /locus_tag="VNT12_00880"
     CDS             complement(1278..2435)
                     /locus_tag="VNT12_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWI44691.1"
                     /translation="MMNKYRLMSPGPTPIPPEVSAAGALPIIHHRTPEFGEVFTRVNE
                     NLKRVFLTENDIFTYAASGTGAFEGAIQNLFSPGDKVLVVNNGNFGGRWVKMSRAFGL
                     EVTELEYEWGQKADNDEVAEALANDPGIKAAICVLSETSTGTVNDIEGFAKAAADVVT
                     IVDAVSGLGACTLRTDEWDVDVVVAGSQKALMTPPGLGFVSVSPRAMRMHEESLMPRY
                     YFDWTAARKAYQKDPAQTAWTPAVSLIIQLDVALQQLIDEGIENVFGRHVLLGRASRA
                     GIKGMGLELFGPDEDPNSAVTAAWVPEGIDGKQLVRMVFREHGIQVAGGQGEMAGRIF
                     RIGHCGYFDAFDIISTIAATELALESLDYPVELGKGVGAAQRVFSKCRVRA"
BASE COUNT          523 a         1013 c          911 g          590 t
ORIGIN      
        1 cccgaagagc gtcccgctca ccgcgttctc acccatgcgc aactccacga ggttcgtgta
       61 gtccgcgctc tccgaggacc gcgaggtctc gaccttgagc ccccgctcct tcgcgagcgc
      121 gggcgtgttg acgaagttca agggctcgaa gaccatcggc gcgaggagtc ctttctgcgc
      181 cgagacgtcg aggaggcgtg tatcgaatcc tgcgacctcg cctcggtact cgatcctgag
      241 cgtatcatcg gtgcggtccg tgagctggta gagcagactc cccagagact cgcacaaccc
      301 cgagaactgg gccacgaact cggcgccctc ccccatcggc acgggggcgt ttatggcgtg
      361 tacaggcacg gcgccactga gggcggccgc cacctgctcg gcagccgtga tgcctgccct
      421 gtcctgggcc tccgcggtgc tcgcccctag gtggggcgtc acaaccacgt tggagagcgt
      481 gaagatcggg gagtccgtcg ccggctcctc ggcgaatacg tcgagcgcgg caccggcgat
      541 ggcgccttcc tttagggcgt tgtagagggc ggtctcgtca acgattccgc cacgggccac
      601 gtttatgagg tacgcggtag gcttcatctt cgccaaagcg tcctcgctca cgagccccat
      661 cgtctgggga gtgcgcggaa cgtgtagcga gacgaagtcg gcccgctcga agacctcgtc
      721 agcggtctcg gcccgctcga cgttcatcga gtgcatcctg tcctctgaga cgtagggatc
      781 gtaggcgagt acctgcatcc ccatacccag tgcgcccctc gccacgatgg cgccgacgtg
      841 accgagcccg acaagcccga tcgtcttccc cgacacctcg acacctttga gcgcgctccg
      901 cttccactcc ccaccccgca aggatgagtc cgcggcaggg atgcgccgcg ctaccgcaag
      961 catgagtccc aatgtgtgct cggccgcggc gaccgtattg ctttcagggg cgttggcgac
     1021 gatgatgccg cgcttggtag cggcctcgat gtctatgttg tccacgccga tgccggcgcg
     1081 tccgatagct ttgaggttcc cggcccgttc tatcacctcg gcggtgactt tcgtagcgct
     1141 gcggatgatg aggccgtcgt aatccccgat cttctggagc agttcttcag gcctgaggtc
     1201 gaggaggacg tccacctcga agtcttgctc tagtagctct atgccgggat cggcgagcct
     1261 ttcggggacg agaaccctca agccctcacc ctgcacttcg agaagaccct ctgggctgcg
     1321 ccgacgccct taccaagctc cacggggtag tcgagggact ccagcgccag ctccgtggcc
     1381 gctatcgtgg agatgatgtc gaaggcgtcg aagtagccgc agtgtccgat gcggaagatc
     1441 ctgcccgcca tctcaccctg cccgccggcg acctgtatcc cgtgctcgcg gaacaccatg
     1501 cgcacgagct gcttaccgtc tatgccctcg ggcacccagg ccgcggtgac ggccgagttg
     1561 gggtcctcgt caggcccgaa gagctcgagc cccatgccct tgatccccgc cctcgacgcc
     1621 cgaccgagca atacgtgccg cccgaaaaca ttctcgatgc cctcgtctat gagctgctgt
     1681 aacgcgacgt cgagctggat gatgaggctt accgcagggg tccacgccgt ctgagccggg
     1741 tccttctggt aggccttcct ggccgccgtc cagtcgaagt agtagcgcgg catcagcgac
     1801 tcttcgtgca tccgcatggc cctcggacta acgctcacaa accccaggcc aggcggcgtc
     1861 atgagcgcct tctgcgagcc ggcgacgacg acgtcgacgt cccactcgtc ggtcctcaac
     1921 gtacaggccc cgagtcccga gaccgcatcg acgatggtga cgacgtccgc ggcggccttc
     1981 gcgaagccct cgatgtcgtt gacggtgccc gtcgaggtct ccgagagcac gcagattgcg
     2041 gctttgatcc cagggtcatt cgccagcgct tcggcgacct cgtcgttgtc cgccttctgg
     2101 ccccattcgt actccagctc ggtgacctcc agtccgaagg cccggctcat cttgacccac
     2161 cgtccgccga agttaccgtt gttgacgaca agaaccttgt ctcccggcga aaacaggttc
     2221 tggatcgctc cctcgaaagc cccggtcccg ctcgccgcgt aggtaaagat gtcgttctct
     2281 gtcagaaaga cccgcttcaa gttctcgttc accctggtga acacctcgcc gaactcaggc
     2341 gtacggtggt gaataatggg tagcgccccc gccgcggaga cctctggcgg tataggagtg
     2401 gggcctggag acataagacg atacttgttc atcatgggtg ctacctctct taccgcgaac
     2461 aacagaacgt ctattttaga catgatcgtt gccgatggct acatgatggg acatctcctt
     2521 tacaaagtgt tgctttcgct atcagcgatt cagctcgtcg cctgttccag gcgacttgtc
     2581 agcacttcaa ctggcgcatg cgaaagccct agctgactgg ctgacaaggc ggcgtagccg
     2641 ccgatgagcc tgtaagcgcc cttacaggcg acgcccgagc gcggcttggg aagcaggctc
     2701 aggtcttcgt tagatgccat gccttcgatt ggtctcccct ctctgagcgt ccgttatccc
     2761 accaagtgca tgatgtcacg tggtaaaggt cggcgcatcc tgcgcaacgc agcctggggt
     2821 acctttgcgc agtatgagcg agaaaaccgg ccgggatgac aggctaaccg aagccagcac
     2881 cctccaacgc aggtagcgcg ttccgcgcgt aacgggcggt gtaagcttgg cctgcgctgt
     2941 tggggaactc gccgggctcc ctgctagtga actccctggc cccggtgcgg atgcggccct
     3001 cggcgagatc tatcctcagg gtatcaccat cacggag
//
LOCUS       DATMJA010000038         3208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_302021, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000038 DATMJA010000000
VERSION     DATMJA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302021"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..892)
                     /locus_tag="VNT12_00885"
     CDS             complement(176..892)
                     /locus_tag="VNT12_00885"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HWI44692.1"
                     /translation="MDAARGVAIIMMIVYHSTYDLDTLGGYGIRSTTGYWALFADVTA
                     GLFLFLVGVSLAISRARTSLTGWRLFGKYLARGLRILAYGVVLTVVFLALGMGVVAFG
                     ILHLIGISIILAYPFLGLRFTNLVLGVIIFAAGQYILAQDLYSQGFWLLPFGVVPEGV
                     IMPDYRPLLPWFGVVLIGLFFGNVVYGDGRQPAVREDKAPVLAKPLLPLGRNSLFVYL
                     IHQPIIIALLALTGVVDLNF"
     gene            1004..1483
                     /locus_tag="VNT12_00890"
     CDS             1004..1483
                     /locus_tag="VNT12_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI44693.1"
                     /translation="MSEDFTIRPGRREDAAEAARLWMQSAKEHTAHDRVYETASGAEK
                     TMRRFLADVANSGYSFLFVAAAGDRTVGFISGELRQGSPTFLPKTWASVDDVFVEPEY
                     RNRGMGRALLKSVQSWAQERGADGISLQVAAANSRGRKFYEDLGFRDVSVYKVLEFE"
     gene            complement(1489..1953)
                     /locus_tag="VNT12_00895"
     CDS             complement(1489..1953)
                     /locus_tag="VNT12_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI44694.1"
                     /translation="MRPVEAALPTGLAEPLALLEEALRHGDPVPEYFADRLRKAVEAG
                     DVEVLAARAEDQILGVALLAYRLNVSAGGLFASIEDLYVRPEARRRGVGRAMLKAVDE
                     LCAERGISYVEAQVEEKEAELFYAALGYEPESGVRVLSRSLSIIDDQDLPET"
     gene            complement(1950..2969)
                     /gene="ltaE"
                     /locus_tag="VNT12_00900"
     CDS             complement(1950..2969)
                     /gene="ltaE"
                     /locus_tag="VNT12_00900"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HWI44695.1"
                     /translation="MIDLRSDTVTRPTEGMRRAMLEAPVGDDVFGEDPTVNRLEEYVA
                     DLLGKQAALYAPSGTMTNQVGVQVNTNRGEEVLLHEGSHIFLYEAGAPAMLSSVQLRI
                     LPGENGLIDPETVRAAVRPEDVHFPRSRLLCLENTHNTSGGKVFPLEGFAAVATTAKE
                     LGLKVHLDGARLFNAQAATGVPASEWCAHADTVSVCSSKGLGAPVGSLLAGDEETIIE
                     ARRVRKAFGGGMRQAGIIAAAALYAFENHVDRLGEDHEHASNLASGLREAGYEAQEPE
                     TNLVLVAVDDPPEFLQALAREGVLATPGKPGYVRLCTHLDVGEREIEAAIQAAARITT
                     SAIER"
     gene            complement(2966..>3208)
                     /locus_tag="VNT12_00905"
     CDS             complement(2966..>3208)
                     /locus_tag="VNT12_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44696.1"
                     /translation="DDAAVAAASIVARARQLEEIERLSERVGFRLPLGATHVVEAGRR
                     VVGELGENGLAVVAKIHFATTRQVLGTAEQSDGGDI"
BASE COUNT          589 a         1028 c         1006 g          585 t
ORIGIN      
        1 gtcctggcgc ttgtatttac tctctgtccc gcaagctcca acgatcccat cgcgctcgct
       61 ggtcatctac tgcgaatatg atctcctact agctacctcc ccatcctcta cggaacgacg
      121 ccgctaactg taacggttac gccgcgaaaa agaaaccaac cgccctgaag ctctactaga
      181 agttcagatc cacgacgcct gttaaggcga gcagggcgat aatgatgggc tggtgtatca
      241 ggtagacaaa cagcgagttt ctgcctagcg gaagaagcgg cttcgccaat accggagcct
      301 tgtcctcacg gaccgcgggc tgtctgccat caccgtacac cacgttcccg aagaacagcc
      361 cgatcagaac gacgccgaac cagggcaata agggcctata atccggcatt atcaccccct
      421 ctggtacgac gccgaagggc aagagccaga agccctgtga gtagaggtcc tgggccaata
      481 tgtactggcc ggcggcgaat atgatgacgc cgagcactag gttggtgaac cggagcccca
      541 gaaaagggta ggcgaggatt atggagatcc caatcaggtg cagtatgccg aacgccacaa
      601 cgcccatccc gagagccagg aagacgacgg tcaacaccac tccgtaagcc agaatcctca
      661 agcccctcgc caggtacttg ccgaagagac gccatcccgt caggcttgtc ctcgcccggc
      721 taatggcgag cgagacgccg actagaaaca gaaagaggcc tgctgtaacg tcggcgaaga
      781 gtgcccagta ccccgtggtg gaccggatgc cgtagccacc gagggtgtcc aggtcgtagg
      841 tggaatgata gacgatcatc atgatgatgg cgacgccgcg ggccgcatcc acctcccaga
      901 acctgtcgac tttcctctcc gtacccggct cctctacggt ttttccggat cataccccac
      961 ccccggcccc cgcgacctgc ggcgcatggg ttaggattgc tttatgagtg aggacttcac
     1021 catcaggcca gggcgcaggg aggacgcggc cgaggcggcg cggctgtgga tgcagagcgc
     1081 caaggagcat acggcccacg accgggtcta cgagacagcc tccggcgccg agaagacgat
     1141 gcggcgcttt ctggccgacg tggcgaacag tggctactct ttcctcttcg tggccgcggc
     1201 gggggatcgg acggtgggct ttatctcggg cgagctgcgc cagggctccc cgaccttcct
     1261 cccgaagacg tgggcctccg tagatgatgt tttcgtcgaa cccgagtacc ggaaccgcgg
     1321 catgggccgc gccctcctca agagcgtcca gtcctgggcg caggagaggg gcgcagacgg
     1381 tatctcactc caggtcgccg ccgccaactc gcggggccgg aagttctacg aagacctggg
     1441 gttccgtgat gtctccgtct acaaagtgct ggagttcgaa tagaaaaatc aggtttcagg
     1501 tagatcctgg tcgtcgatta tggataggga gcgggagaga actcgtacgc ctgactccgg
     1561 ctcgtagccc agggcggcgt agaacagttc ggcttctttt tcttcgacct gtgcttcaac
     1621 gtaagagatg ccgcgctccg cgcaaagttc gtctacggcc tttagcatcg cgcggccgac
     1681 gccccggcgt ctggcttcgg ggcgcacgta gagatcttcg atgctggcga agagcccgcc
     1741 ggcggagacg ttgagacggt aggcgagtag ggctacgccc agtatctggt cttccgccct
     1801 ggcggccagt acctccacgt ctcctgcctc gacggccttt cgcaggcggt cggcgaaata
     1861 ttcggggaca ggatcaccat gcctgagagc ttcttcgagg agcgccaggg gttcggccag
     1921 gcctgttggg agggcggcct cgaccggcct caacgttcga ttgcgctggt agtgatcctg
     1981 gccgctgcct ggatggcagc ctctatctcc ctttccccaa catcgaggtg cgtgcacaaa
     2041 cggacgtagc cgggtttgcc aggggtggcg agcacgcctt cacgggcgag ggcctgcagg
     2101 aattcgggag ggtcgtcgac ggcgacgagg acgaggttgg tctcgggttc ctgggcctcg
     2161 tagccggcct ctctcagtcc ggaggccaga ttgctggcgt gttcgtgatc ctcgccgagc
     2221 ctgtctacgt ggttctcgaa ggcgtagagg gctgcggcgg cgatgatgcc ggcctgcctc
     2281 ataccaccgc cgaaggcctt gcggacccgg cgggcttcta tgatggtctc ctcgtcaccg
     2341 gccagcaggg acccgaccgg cgctccgaga cccttggagg agcaaacgga gaccgtgtcg
     2401 gcgtgcgcgc accactcgct ggcggggacg ccggtcgcag cctgcgcgtt gaagagcctg
     2461 gccccgtcga ggtggacctt caggccgagc tctttcgccg ttgtggcaac cgcggcgaag
     2521 ccctcgagcg ggaagacctt gccgcctgag gtgttgtggg tgttctccag gcagagcagc
     2581 ctggagcgcg ggaagtggac gtcctcgggc ctgacggccg cgcgcacggt ctcagggtcg
     2641 atgaggccgt tctccccggg gaggatgcgg agctggacac tggagagcat cgccggagcg
     2701 ccggcctcgt agaggaagat gtgggaacct tcgtgcagga gcacctcttc tccgcggttc
     2761 gtgttgactt ggaccccgac ctggttggtc atcgtccctg aaggcgcgta cagcgctgcc
     2821 tgcttgccca gcaggtccgc cacgtactcc tcgagccggt tcaccgtcgg gtcctcaccg
     2881 aatacgtcgt ccccaacagg cgcctcgagc atcgcccgcc gcataccctc cgtaggacgg
     2941 gtcaccgtgt cgctgcgaag atctatcata tgtcgcctcc atcactctgt tcggccgtcc
     3001 ccagcacctg acgcgtggtg gcgaagtgga tcttcgccac cactgccagg ccgttttccc
     3061 ctagttcccc gaccacccgc cgaccggcct ccacaacgtg cgtcgctccg agcggcaatc
     3121 tgaaccccac ccgctccgag agacgctcga tctcctcgag ttgccgcgcc cgcgccacta
     3181 tcgacgcagc agccacggct gcatcgtc
//
LOCUS       DATMJA010000039         6775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_835222, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000039 DATMJA010000000
VERSION     DATMJA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835222"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..380
                     /locus_tag="VNT12_00910"
     CDS             168..380
                     /locus_tag="VNT12_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44697.1"
                     /translation="MTVRVRERHRIAERRGMVGRIAAHYGGDGYMAVDVRFSGGHRRL
                     FWPEDLEEISSPRPWWRSLLGRASPE"
     gene            complement(435..1109)
                     /locus_tag="VNT12_00915"
     CDS             complement(435..1109)
                     /locus_tag="VNT12_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI44698.1"
                     /translation="MAKRRITVLLADDHAMFRQGIREMLSTDEEIEVVGEAENGREAV
                     ALAEKLTPDVVLLDVEMPVMGAKQAMERMMENSPPPRVVIVTMYDDPRLVRELIGLGA
                     VAYLVKSATMEELHAAVHTAANSPVGPEKDVIIVAPPNAFRNLAEADGLSERELEVLL
                     MVARGMSNHQIAVSLHLSEATIKRHLANIYPRIGVSSRGEAVRRALSNRWITARDITG
                     GEAREV"
     gene            complement(1111..1815)
                     /locus_tag="VNT12_00920"
     CDS             complement(1111..1815)
                     /locus_tag="VNT12_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWI44699.1"
                     /translation="MLGVCIDVTEQKQAEQERERLHSLEVGAYAEAAERERISRELHD
                     RVAHSMGVAYQSLQLYEALAEKDPVRDRGKLHTAKEMTKTALEQTRNLSMELRRTETA
                     NGLVPALQDLLEVAVPDDVNAVLSTSGAESLLSDHQRGQLYLILREAVRNSVRHSGCR
                     RLTVVGLDITSEEVSGYVEDDGRGFEGNGGSRDHFGLRSIRERTALLEGTAEVYSSPE
                     GGVGVQVRIPLRNGGG"
     gene            2039..2284
                     /locus_tag="VNT12_00925"
     CDS             2039..2284
                     /locus_tag="VNT12_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44700.1"
                     /translation="MDEESHKTYQELWEQYIEVERRELEQRRNGQLGRAIGRALPGES
                     HEELERIAEEDRPARIESENARAAWIQERLRRQPRPQ"
     gene            2486..2965
                     /locus_tag="VNT12_00930"
     CDS             2486..2965
                     /locus_tag="VNT12_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44701.1"
                     /translation="MDGFRRLQDETGIHPGEIVGLPEGGDGTGTVFVLTSSGLRHMGL
                     RPGSARSNATGGGFLGYLQEELLWKDAFELVHEEDLPLLCFLVSLVMEEPGASESAEV
                     RFRNAWGDWVLMDVCVLNVLEAPTGNDTGLLVVNVRQTRESSSWRAEMPNGNQQAPG"
     gene            2958..3254
                     /locus_tag="VNT12_00935"
     CDS             2958..3254
                     /locus_tag="VNT12_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44702.1"
                     /translation="MVRFQHAADFVKSYYTEDSRQVTDNCPVPVLIAGGPRMETAQET
                     LRVVQDATEAGAAGVVFGRNIWQSGDTRGMIRALNSIIHEGRPVDEALAESDLA"
     gene            3318..4847
                     /locus_tag="VNT12_00940"
     CDS             3318..4847
                     /locus_tag="VNT12_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWI44703.1"
                     /translation="MNTEPILIGADVGTTNIKVVAFDRSGRTLQGASSPTPTHFPKPG
                     RAHYDPKELWNSFTATLRQVTEKLEDPGRVASIAVASMGEAAVPLDSSGKPTHHIIAW
                     FDGRAGPQAERLGRVVGQDRLFGLTGLSLQPIFGLCKLLWLKENEPDAYSRTATWLNV
                     ADYMAFRLCGVAATDYSLASRTLALDLDGLRWAGELLEEVGVSPDLFAPLRGGGSFLG
                     PVTSDAASATGLPESAGVAAGGHDHVCGALAVGVTQKGTMLNSLGTAEAIFLPLERPL
                     KDPQVGHQGYTQGAHVAGQYYVFGGQYTSGASVEWLREALGGVDYDTLIAEAEEVPPG
                     SLGAFFLPHLRLANPPYDDPAARGAFVGLSTDVKRGALFRAVLEGLAYDSRNSLEPLL
                     AHSGIDELRAIYAIGGGTQNRLLMRIKATVSNQAITVVGVEEATSLGAAILGGLGAGV
                     YADVPSALQELRYAESRVEPIQEEVSFYDDAFRRVYGRIYPSLRGVHHEIQALQSQHF
                     G"
     gene            complement(4990..5262)
                     /locus_tag="VNT12_00945"
     CDS             complement(4990..5262)
                     /locus_tag="VNT12_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44704.1"
                     /translation="MTFGKNRPPLVPDPGQRVLLLSEDGSRIEGFRAISGPLTAETGE
                     IIIRVATEEEYKDSTKEGRRAVSMAWPAAMVEVSVPWYKWQRWFTR"
     gene            complement(5284..>6775)
                     /locus_tag="VNT12_00950"
     CDS             complement(5284..>6775)
                     /locus_tag="VNT12_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWI44705.1"
                     /translation="KVYKDAAISGTVPLQERPAGRELLEDAKAGIFDVMLVYKLDRVG
                     RTLLVVVDAHDRLMEAQVALRSATEPIDTSTPAGRLIFQMLASFAEFEKETINERTQA
                     GKNRAYRNGVQPGLIPYGFDVGEDGSFVVIEEEAVVVRDIIRNIAGGATLFAEAKRLN
                     LEGIPSPGKKYRGRPRQHGTTWTPMAISRIAGRRAYSGTHVVHSSTGEIEREVPVVVP
                     RELQEIAIARLGDNKRYSGGRPVRKYLLRGLIRCAKCDWNYSGLSRKQGGRYHFKYTC
                     PAATTRRFDPHVRRGGCPWLDALWLEDLVWHDIRGFVENPGEVLERVKEQMEERRTQT
                     GELEERIASLTKRLATVQGEKDKYVKLYAAGHLDEEELEVHLLDVKNRISNLKMLIES
                     AEAGLAREEQDAAAAKNTATWLTSLRGGLQALEEHTDEAWHDRRELVEQLVEKITVSR
                     TEDGRAKIHIAYKFAPPVQSASGITDHKTFEILAGEIPIPEALDRL"
BASE COUNT         1212 a         2162 c         2083 g         1318 t
ORIGIN      
        1 agagacgcct attgtatctg atgatactga catctcgacg gctatcggtg aagattttgg
       61 ggcattggga cttcgccgct ctgtatgcgg atgagtgaat gcttgggaga ggaagattct
      121 gtcgcctggc ggggggcgcc ataaagccgg aaaaagtgct tattggtatg acggtaaggg
      181 tgcgcgagcg tcataggatc gccgagcgac gcggcatggt gggtaggatc gcggcccatt
      241 acgggggaga tgggtacatg gccgtcgacg tccgcttctc gggcggacac aggaggctgt
      301 tctggcctga ggacctggag gagatctcct caccccgacc gtggtggcgt tcgctactcg
      361 gtagagctag cccagagtga tctgtagcct aacgccaggc atgaggaagg ctccggagcc
      421 ttcctcatgc cgctctagac ttctcgcgcc tctccgccgg ttatatcgcg ggctgtgatc
      481 caacggttgg agagggccct tctcacagcc tcaccgcggg aagagactcc gatcctggga
      541 tatatgttgg ccaggtgacg ctttatggtc gcttctgaga ggtgcaatga gacggcgatc
      601 tgatggttgc tcatcccgcg ggcgaccatg agcaatacct ccagctcccg ctcggagaga
      661 ccgtcggcct cagccaggtt cctgaacgca ttgggagggg cgactatgat gacgtccttt
      721 tcgggcccga cggggctgtt tgcggcagtg tgaaccgccg cgtgcagttc ctccatggtg
      781 gcgctcttta ccaggtaggc tacggcaccg agtccgatca actcgcgcac cagcctgggg
      841 tcgtcgtaca tggtgacgat aaccaccctg ggaggcggcg agttctccat catgcgctcc
      901 atcgcctgct tcgcacccat gaccggcatc tccacgtcaa ggagcaccac atcgggcgtg
      961 agcttctcgg cgagggcgac agcctcccgt ccgttctccg cctcaccgac gacctctatc
     1021 tcctcgtcgg tggagagcat ctcccttatg ccctgacgga acatggcgtg atcgtcggcc
     1081 agcagcaccg ttatccttct ttttgccaac ctaccctccc ccgttccgaa gcgggatacg
     1141 gacctgtact cccacgccgc cttcagggga agagtacacc tcggctgtac cttccagaag
     1201 tgctgtgcgt tcccttatgg aacggagccc aaagtggtct cgagacccgc cgttgccctc
     1261 gaagccgcgg ccgtcgtcct cgacgtatcc agagacctcc tccgaggtta tgtccagccc
     1321 gacgaccgtc agtcggcggc aacctgagtg cctgacagag ttgcgaacgg cctcacgcag
     1381 gatcaggtag agttgtccgc gctggtggtc cgagagcagc gactccgctc ctgaggtgga
     1441 gagcaccgcg ttcacgtcgt caggcacggc gacttccagg agatcctgca aggcaggtac
     1501 caagccgttc gctgtctccg tgcgccgcag ctccatggag aggttcctgg tctgctccag
     1561 cgcggtcttc gtcatctcct tcgccgtgtg tagctttccg cggtcccgca cggggtcttt
     1621 ctccgccagc gcctcgtaga gctggaggct ctggtaggcg acgcccatcg agtgggccac
     1681 cctgtcatgg agctcccggc tgatcctctc ccgctcggcg gcctcagcgt aggcgccaac
     1741 ctccaaagag tggagccgtt cgcgctcctg ctcggcctgc ttctgttctg tgacgtcgat
     1801 gcagacccct agcacccgcg cgggtgttcc tctctcgtcc ccctgaaaaa cgacccctgc
     1861 aagcacaggt catcaaccat acattctaca ttgctacgaa cgcgggtgcg accgcatgac
     1921 cggtgttgtt ggatgtacaa tgcggtagct gaagacgtca agtcgttgcg ctggctgcgc
     1981 ctggtttcgt acagcacctc aggtcgaggg ctgatcgaag accgacaaga ggaagcaaat
     2041 ggacgaggaa tcacacaaga cgtaccagga actgtgggaa cagtacatag aggtcgaacg
     2101 cagggagctg gagcaacgta gaaacggaca actgggccgg gcgataggcc gcgcgctccc
     2161 cggcgagtcg catgaggagc tagaacggat agccgaggag gaccggccag ccaggataga
     2221 atcggagaac gccagggcgg cctggatcca ggaacgcctg cgccgccagc cccgcccaca
     2281 gtaaggtaaa tggggggaga cggttccgta tccccggttc gaaggctcta tatttcctgc
     2341 atactttggg ccatttttct cgcccgtcgc taccgaggac tcgttgtgcg atggcggacg
     2401 tgctattagt actgaaatag tcggcagtgg gtaatcccgc gccgcttggg gcgtcatcga
     2461 cgggttggct gcaaggggaa tatgaatgga cggctttcgc aggctgcaag acgaaacagg
     2521 gattcatccg ggagagattg tgggcttacc tgagggaggg gatgggactg gcacggtgtt
     2581 cgtcctgacc tcctccggcc tgcgccacat gggcctccgg cccgggtcgg cccgctcgaa
     2641 cgcaacaggt ggagggtttc tcggctacct tcaggaggag ctcctctgga aagacgcctt
     2701 cgagctggtc cacgaagaag atctgcccct actctgcttt cttgtctcat tggtcatgga
     2761 ggagccggga gcctcggaga gcgcggaggt ccgtttccgc aatgcatggg gagattgggt
     2821 tctcatggac gtctgcgtgc tgaacgtgct cgaggccccc acaggtaacg acacgggact
     2881 actagtagta aacgtgcgcc agacgagaga aagttcgtct tggcgcgccg agatgcccaa
     2941 cgggaaccag caggcgcctg gttaggtttc agcacgccgc tgacttcgtc aagtcctatt
     3001 acacggagga ctcccgccag gtgacggaca actgccccgt gccagtcctg atcgccggcg
     3061 gtccgaggat ggagaccgca caggagacgc tacgggtcgt ccaagacgcc acagaagccg
     3121 gcgccgcagg cgtcgtcttc ggccgcaaca tctggcagag cggcgacacg cgaggcatga
     3181 tccgcgccct gaacagcatc atccacgaag gccgccccgt cgacgaggcg ctcgccgaat
     3241 cagatctggc atagtgtata gagaggctgg agcaagttga catcgggctg cctgacctcg
     3301 aaaggctcta ccacccgatg aacacggaac cgatcctcat cggggccgac gtcggcacga
     3361 cgaacatcaa ggtcgtggcc ttcgacagga gcggcaggac actacagggg gcaagctcgc
     3421 ctaccccgac ccacttcccg aaaccgggca gggcccacta cgaccccaag gagctgtgga
     3481 actcctttac ggcgaccctg cgccaggtca cggagaagct cgaagacccc ggccgcgtgg
     3541 cgagcatagc cgtcgccagc atgggtgagg cggccgtgcc gctcgactcc agcggcaagc
     3601 ccacccacca catcatcgcc tggttcgacg ggcgggcggg gccgcaggcc gagcggttgg
     3661 gccgcgtcgt cggacaggac cgtctcttcg gcctgacggg cctctcgttg cagcccatct
     3721 tcggcctgtg caagctactg tggctcaagg agaacgagcc cgacgcatac tcgcgcaccg
     3781 ccacctggct gaacgtggcc gactacatgg cgttccgcct gtgcggcgtc gccgccacgg
     3841 attactccct ggcctcacgc accctggccc tcgacctaga cgggctgcgg tgggccgggg
     3901 agttgctcga ggaggtgggt gtctccccgg atctcttcgc acccctccga ggtggcggct
     3961 cgttcctcgg tcccgtaacc tcggacgccg cgtccgctac agggctgcca gagagcgccg
     4021 gagtcgcggc cgggggccac gaccacgtct gcggcgccct tgccgtcggc gtcacgcaga
     4081 agggcacgat gctcaactcg ctcggtactg cagaggcgat cttcctcccg ctcgagcgcc
     4141 cgctcaaaga cccccaggtc gggcatcagg gttacactca gggcgcccac gtggcaggcc
     4201 agtactacgt cttcggcggc cagtacacct cgggcgccag cgtcgaatgg ttgcgagagg
     4261 cccttggcgg ggtcgactac gacaccttga tcgccgaggc ggaagaggtc ccgccgggtt
     4321 ccctcggcgc cttcttcctg ccgcacctgc gcctcgcgaa cccgccgtac gacgaccccg
     4381 cagcgagggg ggcgttcgtc ggcctcagca cggacgtcaa gcgcggagcc ctcttccggg
     4441 ccgtcctcga gggactggcc tacgactccc gcaactcgct cgaaccactc ctcgcccact
     4501 cagggataga cgagctcagg gccatctacg ccatcggcgg cggcacccag aaccgtctgc
     4561 tgatgcgcat caaagccacc gtctcgaacc aggcgatcac cgtagtcggg gtagaggagg
     4621 cgaccagcct gggggccgcc atcctaggtg ggttaggcgc cggagtctac gccgacgttc
     4681 cgtcagccct ccaagagttg cgctatgccg agagtcgcgt cgagccgatc caggaggagg
     4741 tctcgttcta cgacgacgcc ttccggaggg tgtatgggag aatctacccc agcctccgtg
     4801 gcgtgcacca cgagatccag gccctgcaga gtcagcattt cggctagtca gcacgcttcg
     4861 gccttcgggc ttcgcttgtc agcacggcgg ctgcgccgcc ttgtcagcta gtcagccaag
     4921 acttgcgcat ccggaagctg aagtgctgag aagtcgcctg gaacaggcga cgtgctgaaa
     4981 tgctgacacc tacctcgtaa accaccgctg ccatttgtac cagggcacag acacctccac
     5041 catcgcggct ggccaggcca tgcttacggc gcggcgtccc tccttcgtcg aatccttgta
     5101 ttcctcttcg gtcgccaccc tgatgattat ctcacccgtc tccgcggtga gtggtccgga
     5161 gatggcacgg aatccttcga tgcgcgagcc gtcttcggac aacaacagca cccgctgtcc
     5221 aggatcgggc acgaggggag gcctgttctt gccgaaagtc atctcttcac caattgtatc
     5281 cgtctagagc cggtcgagag cctcggggat cgggatctct ccggccagga tctcgaacgt
     5341 cttgtggtcc gttataccac tcgcagactg tacgggcggc gcgaacttgt aggcgatgtg
     5401 tattttggct cggccgtctt cggtgcgact tactgtgatc ttctcaacga gctgttcgac
     5461 caattcgcgc cgatcatgcc acgcctcgtc tgtgtgctct tcgagtgctt gcaggccgcc
     5521 gcgcaaggac gtgagccacg tggccgtgtt ctttgccgcc gcggcatcct gctcttctct
     5581 ggcgaggccg gcctcggccg actctatcag catcttcaaa ttcgagatac ggttcttgac
     5641 gtccaaaagg tgaacctcca gctcttcctc gtctaggtgc cccgcggcgt agagtttgac
     5701 gtacttgtcc ttctcgccct ggaccgtcgc caggcgtttt gtgagcgatg cgatcctctc
     5761 ctcaagctcg ccggtctgtg tgcgcctttc ttccatctgc tccttgaccc gctccaggac
     5821 ttcgccgggg ttctccacga atccccggat atcgtgccat acgagatcct ccagccacag
     5881 agcgtcgagc cacggacatc ccccgcgccg cacgtgcggg tcaaatcgtc tcgtcgttgc
     5941 ggcagggcat gtgtatttga agtgatagcg gccaccctgt ttgcgtgaca ggccgctgta
     6001 gttccagtcg catttagcgc acctgataag acctcgaagg aggtatttgc gaacggggcg
     6061 ccctccggag tagcgtttgt tatcccctag gcgggctatg gcgatctcct gcaactccct
     6121 ggggactacg accggcactt cacgctctat ctcgccggta gaggagtgta ctacgtgggt
     6181 gccggaatat gccctgcgcc ccgcgatgcg gctgatcgcc atgggggtcc aggtcgtgcc
     6241 gtgctggcgc gggcggccgc ggtacttctt gcccggcgaa gggatgcctt ccaggttcaa
     6301 acgcttcgcc tccgcaaaaa gtgtagcgcc ccccgcgatg ttacggatga tgtcccggac
     6361 gacgacagct tcttcttcaa tcacgacgaa agacccatct tccccgacgt cgaagccata
     6421 agggattaat ccgggctgta cgccgttgcg gtaggcccga ttcttgccgg cctgggttct
     6481 ctcgttgatg gtttcctttt cgaactccgc aaagctggcg agcatctgga aaatgagcct
     6541 ccccgccggc gtggaggtgt ctatgggctc tgtggcgctg cgcaaggcta cctgcgcctc
     6601 catgagcctg tcgtgggcgt ccaccaccac aagcagcgtc cgaccgaccc tatccagctt
     6661 gtagaccaac atcacatcga atatcccggc cttagcgtct tccagcagtt cacgccccgc
     6721 tggcctctct tgcagtggca cggtgccaga gatcgccgcg tctttgtaga ccttg
//
LOCUS       DATMJA010000040         3078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_242902, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000040 DATMJA010000000
VERSION     DATMJA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242902"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="ribA"
                     /locus_tag="VNT12_00955"
     CDS             <1..693
                     /gene="ribA"
                     /locus_tag="VNT12_00955"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HWI44706.1"
                     /translation="PCGAEGGSAGRGAGVIMSAARRVAQASLPTRYGTFEMFVYDTPE
                     HKEHVALTIGAIDDGRPVLVRVHSECLTGDVLGSSRCDCGEQLNDSLRFLQEQGRGIL
                     LYLRQEGRGIGLTNKISAYALQEQGLDTVEANLALGLPEDMRNYRAAAEMLLDLGVWR
                     ARLLTNNPAKVEGLESYGVEVVERMPLSMSPNPSNLGYLRTKREKMGHLFPEPVALEG
                     RIEYPDDTANED"
     gene            712..1545
                     /locus_tag="VNT12_00960"
     CDS             712..1545
                     /locus_tag="VNT12_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWI44707.1"
                     /translation="MRYGIVVSNLDDYADPRLAIRLARAAEEAGWEAFFVWDHLGFVW
                     GSASSDPWISLSAVAVSTTHLKLGTAITPLARRRPHIVANALASLDLLSGGRMIFGAG
                     LGGVSEEFTAVGEPGAVKQRAAMLDEGLTVLDRLWSGEMVMHHGQYYDVENVSLAPLP
                     FQRPRIPIWIGGESPPALRRAARWDGWLAPTTSPDGTPIMDKDPGRIAEMVAEIRRHR
                     TTDTPFEVAVDGYSGPGDPTLPHAYEAAGATWWLESIHGIRGSLDEMNARVQAGPPEP
                     R"
     gene            1685..2629
                     /locus_tag="VNT12_00965"
     CDS             1685..2629
                     /locus_tag="VNT12_00965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWI44708.1"
                     /translation="MRAIAEETFGGPIALMDLPIPEIGAGEVLIRVRAAGVNPFDWKV
                     ADGALEDQMQHRFPLIPGFDAAGVIERVGADVTELAEGDEVYGYLFKPVIGEGTYAEY
                     VGAPATIVAKKPVTVGFAEAAALPTPGLTAMDLVDAVDTREGETILIAGATGGVGSYA
                     TQLAARRGAHVIATARQTNEALVRRLGAAGTIDHTTEDLVDAVRLAQPGGIDAVIDVV
                     SDSEALGRISTLVHEGGRLASSVYAADVEGLARRGIGATNVSMQPDARRLGELSRMVD
                     SGELSVRLDRTFPLEKAPEALEERKIGHVLGKIVLLID"
     gene            2694..>3078
                     /locus_tag="VNT12_00970"
     CDS             2694..>3078
                     /locus_tag="VNT12_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWI44709.1"
                     /translation="MKHVVTARADIVGSLLRPPELLEARGSYAAGGLSAAEFKRIEDR
                     AVDWAIGLQERAGLPIVTDGEMRRESFQSQLVEAVEGFGEHDMDAWLWGEWHGDEAAG
                     DKTTARPQTLGVVGKLRRRRHLSVEE"
BASE COUNT          587 a          877 c         1052 g          562 t
ORIGIN      
        1 ccgtgcggcg cggagggtgg ttcggcagga agaggggcag gtgtgatcat gtcagccgct
       61 cgacgggttg cccaggcgag ccttccgact cgttatggaa ccttcgagat gtttgtctac
      121 gacacacccg agcacaaaga acacgtggcg ctcaccattg gagctatcga cgacggtagg
      181 ccggttctgg tccgtgtgca ctcagaatgt ctaactggcg atgtcctcgg gtcgtctcgt
      241 tgcgactgcg gagaacagct aaacgatagt ctgcggtttt tgcaggagca gggccgtggc
      301 atcttgctct accttcggca ggaaggccgc ggcatcggtc ttaccaataa gatctctgcc
      361 tacgcgctcc aggagcaagg gctcgatacg gtcgaagcga accttgcgct tggattgccg
      421 gaggacatgc gcaactaccg cgccgccgcc gagatgttgc tcgacctcgg tgtatggcgc
      481 gctcgcctgc tcaccaacaa cccggcaaag gtcgaaggct tggagagtta cggcgtcgag
      541 gtagtggagc gcatgccatt aagcatgtcg ccgaacccct cgaatctagg gtacctgcgg
      601 acaaagcgag agaagatggg ccaccttttc ccggagcccg tggcgctcga aggccggatc
      661 gagtacccgg acgacactgc caatgaggat tgaaagcagg gagcgtatag gatgcgttac
      721 ggcatcgtag tgtctaatct ggatgattac gccgacccgc gcctggcgat ccgcctggct
      781 cgagcagccg aggaggccgg gtgggaggcc ttcttcgtgt gggatcacct cggcttcgta
      841 tggggttcgg cctcatccga tccttggatc agcttgtccg ccgtggcggt ttccaccacc
      901 catctcaagc tggggacggc cattaccccg ctggcgcgcc gtcgtccgca catcgtggcc
      961 aatgccctgg cgagcctgga tctccttagc ggtgggcgaa tgattttcgg ggctgggctc
     1021 gggggggtga gtgaagagtt taccgctgtc ggcgagccgg gcgctgtgaa gcagcgtgcc
     1081 gcaatgctcg atgaggggct cacggtcctg gacagactgt ggtcgggtga gatggtgatg
     1141 caccacgggc aatactacga cgtggagaac gtgtccctgg cgccgctccc gtttcagcgt
     1201 ccgcgcatcc cgatctggat aggtggcgag agcccacccg ctctgcgtcg tgcagcccgc
     1261 tgggatggtt ggctcgcgcc taccacaagc cctgatggaa ctcctatcat ggacaaggac
     1321 cctggacgta tcgccgagat ggtggccgag atccggcgac accggacgac ggacacgccc
     1381 ttcgaggttg ccgtcgacgg atactctggc cctggtgatc cgacgttacc gcacgcctat
     1441 gaggcggccg gagcgacttg gtggttggag agcatccacg gcatacgtgg ctcgctcgac
     1501 gagatgaatg ctcgtgtgca agcgggcccc ccggagccga gatgagtgtt cgatgcgggc
     1561 caggtacgta ctgacgtaag gctcactgca ggtgcaagag tgacgcaaca agtgattacc
     1621 gaacaggcta aaggtacggg ccacaagtag atcttggtgt cctaccggta ttgaaaggag
     1681 attgatgcga gcgatagccg aagagacgtt tggtgggcca atagccctga tggacttgcc
     1741 gatcccggag atcggggccg gtgaggtctt gatccgtgtt cgggccgctg gggtcaaccc
     1801 cttcgactgg aaagtcgcag acggagcgct cgaggatcag atgcagcacc gcttcccctt
     1861 gatcccgggc tttgacgctg ccggagtgat cgaacgagtt ggtgccgatg tcactgaact
     1921 cgctgaaggc gacgaggtat acgggtactt gttcaagccg gtgatcgggg agggcaccta
     1981 cgcggagtac gtcggtgctc cagctaccat tgtcgccaag aaaccggtga cggtcggctt
     2041 cgccgaagcg gcggcgctac ccacgccggg gctcaccgcc atggacctcg tggatgcggt
     2101 ggacaccagg gaaggcgaga ccattttgat cgcgggggca accggtggcg tcggctccta
     2161 cgcgacccag ctggcggcta gacgcggcgc ccacgtcatc gccaccgcgc gccaaaccaa
     2221 cgaggcgctc gtgcggaggc tgggagcagc ggggacgatc gatcacacca cggaggacct
     2281 cgtcgacgcc gtacgactgg ctcaaccagg cggcatagat gcggtcatcg atgtggtaag
     2341 cgacagcgaa gcgctcggcc gcatatccac actcgtacat gagggtggga ggctcgcatc
     2401 gtcggtttac gctgccgacg tggaggggct tgcaaggcgt ggtatcggag cgacgaacgt
     2461 tagcatgcag cccgacgccc ggcggttggg ggagctctcg cggatggtcg actccgggga
     2521 gctctccgtg aggctagatc gcaccttccc gctcgagaaa gcacctgagg cgctcgaaga
     2581 aaggaagatc gggcacgtac ttggcaagat cgtacttctc atcgattgag gagcaattaa
     2641 gctcgtgcaa gcctaggctt ctcgagagac cgcctggtgc tagactgggt tgtgtgaagc
     2701 acgtcgttac cgctcgcgca gacatcgtcg gcagcctcct tagacctccg gaactgctcg
     2761 aggcgcgcgg aagctacgct gcaggcgggc tctctgctgc cgagttcaag cgtatcgagg
     2821 atcgtgccgt ggactgggct atcgggctgc aggagcgggc aggccttcca atcgtcacgg
     2881 acggtgagat gcgccgggag tctttccaga gccagctggt agaggccgtc gaaggcttcg
     2941 gcgagcatga catggacgcc tggctgtggg gagagtggca cggcgatgag gcggcgggag
     3001 acaagactac cgcacgccca cagacactcg gggtggtcgg gaagctacgc cgcaggcgcc
     3061 acctctcggt cgaggagt
//
LOCUS       DATMJA010000041         4256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_65278, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000041 DATMJA010000000
VERSION     DATMJA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65278"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VNT12_00975"
     CDS             complement(<1..1161)
                     /locus_tag="VNT12_00975"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HWI44710.1"
                     /translation="MTRPYEPDAPVSAEEYLASQPPVLEVTDALRKIRERVVASGRRV
                     IVLDDDPTGTQTVHDVPVLTAWSYSELRWALEQPNPVVYVLTNSRSLDEPDAAALNER
                     IGRRLRRAASDSDTRFVVVSRSDSTLRGHYPVETNTLERELGVNVDGVIICPCFFEAG
                     RVTLDDVQWVRHGEELVPAALTEFATDTTFGYSSSNLAGWIEEKTGGRTPAESVLCVG
                     IADIREGGPERITALLQGAKDAQPIVVNAAGYADLEVFVLGLLEAEATGKSFLYRTGP
                     SFVRVRGGIPERPPLEARDLYHGYVGRRHGLVLVGSHVRQTTRQLRTAKKLDGLRTVE
                     LSVTRILDPQRRTEELDRVVRLVNETLSKADVVVYTSREVVRASGDLSGLEVG"
     gene            complement(1281..1421)
                     /locus_tag="VNT12_00980"
     CDS             complement(1281..1421)
                     /locus_tag="VNT12_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44711.1"
                     /translation="MSVLDAAPERFALGAALAVIEDCGYLSSMEQPQAVTALLLYWLQ
                     DG"
     gene            complement(1541..2575)
                     /locus_tag="VNT12_00985"
     CDS             complement(1541..2575)
                     /locus_tag="VNT12_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HWI44712.1"
                     /translation="MTTIVIASYFEEEYVRRIREVDERLRVLYGEDLVPPPRWPGDHA
                     GPEEWERTPEAEGEFLAMLAEAEVLYDFPSGHVEDLVEVAPRLRWVQGSMAGAGEVAQ
                     KAGLGETDVVVTTASGIYSGPLAEFVLMAMLQHAKELDNLRADKAGKIWRQGAVGTLE
                     NKTLCVVGMGSIGRAIAGRVRPFGMRVVGVKRTVREDDEAWDYTDELYPTAELSTALS
                     KADYVAVTLPGTPETRRLLDAEAIAAIKQGAYFANVGRGSVVDEAALAEALQNGRLSG
                     AALDVFEVEPLPEESPLWELDNVIVSPHTTDVVPTLINTAQTDLFCENLRRYLAGEEL
                     LNVLDKRLLY"
     gene            2776..3402
                     /locus_tag="VNT12_00990"
     CDS             2776..3402
                     /locus_tag="VNT12_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWI44713.1"
                     /translation="MEELLDILDESGRPTGEVVPKSETHRLGLWHRCFHCWICGSEPG
                     GSYLLLQRRAATKDTWPGYLDVTAAGHLAAGERTMDGLREVEEELGLRVEPERLVPLG
                     TRRVEQEIPGGCDREFHDVFLLFDATSPGDLRLQKEEVEAVFRLDLDDVEALYEKGSA
                     PAREYAEGRAFATRIHLAEFVPKEEGYLRRVAGAARRHLSGAPSVPIF"
     gene            3639..>4256
                     /locus_tag="VNT12_00995"
     CDS             3639..>4256
                     /locus_tag="VNT12_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWI44714.1"
                     /translation="MADTLERATDSGAELVAYPELTIPGYPPEDLLMRQSFIEENMRA
                     LHEFAGRVPENVVAAAGFVDLGADLYNACALVSGGEVLYRYHKRYLPNYGVFDENRYF
                     REGTGASILDLGGALVGVSVCEDIWYPGGPAREQALGGASVLLNISASPYHRRKGAAR
                     ERMLGVRAADYGCYVLFCNLTGGQDELVFDGHSVVFDPEGRLVARA"
BASE COUNT          726 a         1402 c         1330 g          798 t
ORIGIN      
        1 cccgacctcg agcccagaga ggtctccgga ggcgcgtacg acttcgcggc tcgtatacac
       61 gaccacgtcg gccttggaga gcgtctcgtt gaccagtctg accacccggt ccagctcctc
      121 tgtcctcctc tgggggtcga ggatccgggt cacggacaac tccacggtac gcagcccgtc
      181 cagcttcttt gccgttcgta gctgccgggt cgtctgccgc acgtgcgagc caacgaggac
      241 gagaccgtgc cttctaccga cgtatccgtg gtagaggtcc cgcgcctcta gcggcggtct
      301 ctcggggatg ccgcccctca ctcgcacgaa cgaaggcccg gtacggtaca ggaaagactt
      361 gccggtcgct tccgcttcga gcagccccag tacgaagacc tcgaggtcgg cgtatccggc
      421 ggcgttgacc acgatgggtt gcgcgtcctt ggcgccctgc agcagggcgg taatcctttc
      481 cgggccaccc tccctgatat ccgcgatgcc gacgcacagc acgctctctg cgggggtcct
      541 gccgcccgtc ttctcctcga tccagccggc gaggttcgag gacgagtagc cgaaggtagt
      601 atcggttgcg aattccgtga gggctgcggg gaccagctct tcgccatgcc ttacccactg
      661 cacgtcgtcg agggtcactc ggccggcctc gaagaagcac ggacagatga tgacgccgtc
      721 gacgtttact cccagctccc tttccagcgt gttggtctcg accgggtagt gcccgcgcag
      781 ggtcgaatcg ctccggctca cgacgacgaa ccgggtgtcc gaatcggagg cggcgcgtct
      841 caggcgacgt ccgatccgtt cgttcaatgc ggcggcatcg ggctcgtcga ggctacgcga
      901 gttcgtcagg acgtacacga cggggttcgg ttgctccagc gcccagcgaa gctccgagta
      961 cgaccatgcg gtgaggacgg gaacgtcgtg cacggtctgg gtgccagtgg gatcgtcgtc
     1021 gagcacgatc acgcgcctgc cgctggcgac tacccgctcc cttatcttcc tcagggcgtc
     1081 ggtaacctcc agtacgggtg gctgggaggc cagatattcc tccgcgctta cgggggcgtc
     1141 gggctcgtag ggccgcgtca atgcacgctc cttagcgccc gcctttgacg gctgccacta
     1201 ccctggcctc cgccgagccc acgagtcgct ctgtacggtt ctctcaaagc cgttgcctga
     1261 tcccttccct ctcgaattac ctaaccgtcc tgcaaccagt agagtaacag cgctgtcacg
     1321 gcctgcggct gctccatgct gctcaggtag ccgcaatcct cgataacggc cagcgcggcc
     1381 ccaagggcaa agcgctccgg ggcggcatcc agcaccgaca tggtcccctg gtccgatcct
     1441 tctagtagtc tagcgtaaga ggcttcggaa cctgacaagg cttcgaggcg tttcgcagaa
     1501 cgatgtggct tcggcgagaa caagctgatg cctgaccgct ctagtagagc agacgtttgt
     1561 ccagtacgtt cagcaactcc tcgccggcga ggtaacggcg cagattctcg cagaacagat
     1621 cggtctgtgc ggtgttgatc aaggtcggca cgacgtccgt tgtgtggggg ctgacgatca
     1681 cgttgtcgag ctcccaaagg gggctctcct caggcagagg ctcgacctcg aagacgtcga
     1741 gcgccgcccc tgagagacgg ccgttctgaa gcgcctccgc gagcgccgcc tcatcgacaa
     1801 cggaccccct tcctacgttg gcgaagtacg ccccctgctt gatcgcggcg atcgcctcag
     1861 cgtcgagcaa gcgccgcgtc tcgggggtgc ctggaagcgt aacggcgacg tagtcagcct
     1921 tgctgagagc ggtgctcaat tccgccgtcg ggtacagttc atcggtgtag tcccaggctt
     1981 cgtcgtcctc gcgcaccgtg cgcttgaccc cgacgacgcg cataccgaat ggtcgaaccc
     2041 gccctgctat ggcgcgaccg atactcccca tcccgaccac gcacagcgtc ttgttctcga
     2101 gcgtccccac cgccccctga cgccagatct ttcctgcctt gtcggcgcgg aggttgtcga
     2161 gttccttggc gtgctggagc atcgccatga gaacgaactc ggctaatggc cccgagtaga
     2221 tgccgctggc cgtcgtcacc acgacatccg tctccccaag gccagccttc tgtgccactt
     2281 ctcccgctcc tgccatactt ccctgcaccc acctgagtct gggggcgacc tcaacgagat
     2341 cctctacgtg accgctgggg aagtcgtaga gcacctcggc ctcagcaagc atcgccagaa
     2401 actccccttc ggcttcaggc gttcgctccc actcttccgg tcccgcatgg tccccgggcc
     2461 accgaggtgg cgggaccaga tcctccccgt agagtaccct caaccgttcg tccacctccc
     2521 ttatgcgacg gacgtactct tcctcgaagt agctggctat cacgatcgtg gtcacggtgc
     2581 gtctcctctt ggtaagcatt tcagcacgct tcggcctttc aggcctccgc tagtcagcat
     2641 ttcagcattt cagccttcgc atgcgcaagt cttggcagac atgctgacaa ggcggcacag
     2701 ccgccgtgct gacaagcgga ggcggagccg gagcgtgctg aaatgctgac tagctgaaat
     2761 gctactctta tccatgtgga ggaactgctg gacatcctgg acgagtcagg acgaccgacg
     2821 ggggaggtcg ttccgaagag cgagacgcac cggcttggtt tgtggcaccg ttgttttcac
     2881 tgttggatct gcggttccga acccggagga tcctacctgc tcctccagcg gcgcgcagcc
     2941 acgaaagata cctggcccgg ctacctcgac gtcaccgccg cagggcacct ggcggcgggg
     3001 gaaaggacga tggacggcct gcgggaggtc gaggaggagc taggcttgcg ggtggagcct
     3061 gagaggctcg tcccgctcgg aacccgcagg gtggagcagg agatacccgg cggctgcgac
     3121 agggagtttc acgacgtctt cctcctgttc gacgccacat ctccagggga cctgcgtctc
     3181 cagaaggagg aagtcgaggc tgtgtttcgg cttgatctcg acgacgtgga agcactctac
     3241 gagaagggtt cggcgcccgc ccgcgagtat gctgaaggca gagcctttgc tacgcggata
     3301 cacctcgcgg agttcgttcc gaaagaagaa ggctacctgc gcagggtggc cggagcagcc
     3361 cgtcgacacc tgtcaggagc accgtccgtc cctatcttct agtctgagat ctgcctcacg
     3421 gccagatcgg cccgccagcc gctgcgagag gcctgtcccc gtcggggaga gtgggaaggt
     3481 tgcaggcggc aagggtggcc gggtggcccc aggctatgcc ccacaggcat gaacatcggc
     3541 cggcacgaac gcaccaggcg cgatatattt agtgcatgag ggttgcgctg gcccagatca
     3601 acaccaccgt gggagatatc taggggaatg tcgagaagat ggccgacacc ctggagcggg
     3661 cgaccgattc aggcgctgag ctagtcgcct accctgagct gaccatccct ggctatccgc
     3721 ccgaggacct cctcatgcgc cagagcttta tcgaggagaa catgagggcg ctccatgagt
     3781 ttgccggacg ggtgcctgag aacgtggtgg ccgcagctgg cttcgtcgac cttggcgccg
     3841 acctgtacaa cgcctgcgcc ttggtgtcag gcggcgaggt actgtaccgc taccacaagc
     3901 gctacctgcc gaactacggc gtcttcgacg agaaccgcta cttcagggag ggtactggcg
     3961 cctcgatcct cgatctgggc ggcgccctcg tcggggttag cgtgtgcgag gacatctggt
     4021 accccggtgg cccggcccgc gagcaggccc tcggcggggc gagcgtcctc ctcaacatct
     4081 cggcctcccc ttaccacagg cggaagggcg ccgccaggga acggatgttg ggcgtgcggg
     4141 ctgccgacta cggttgctac gtactcttct gcaacctcac gggcggccag gacgagctcg
     4201 tcttcgacgg ccactcggtc gtcttcgacc ccgagggcag gctcgtggcg cgggcc
//
LOCUS       DATMJA010000042         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_361740, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000042 DATMJA010000000
VERSION     DATMJA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361740"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VNT12_01000"
     CDS             complement(<1..215)
                     /locus_tag="VNT12_01000"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWI44715.1"
                     /translation="MQLPPGTKAGRLASGSVPERGRFVERSYTNSAGTRSYKLYIPSG
                     YTGQAVPLIVMLHGCTQTPDDFATGTR"
     gene            412..1374
                     /locus_tag="VNT12_01005"
     CDS             412..1374
                     /locus_tag="VNT12_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HWI44716.1"
                     /translation="MNVLVSGATGFIGSALIPELEGLGHRVVRLSRSKPSAEDTIRWD
                     PASGVLDPSRLRGLDAVVHLAAENVGDGRWTPAKKRRIMDSRREGTHLLAETIAGLPE
                     PPGVMVSVSGINYYGSRGNELLREESGPGNSFLARVCQEWERAADPARSAGVRVVHPR
                     FGIVFPQGFSRDLMGIKAGIEHPKFEITFGTRGSTLARLPVYKLTGGGWIGSGRQFWS
                     LLVAEDVVGVILHALTTDSLQGPVNAVSSSVTAREYIVTLNRILNRPTVFPLPAPVAR
                     LALGDFAEEQLLASIRVDSTKLTDSGYEFRYPELAGALRHLVEE"
     gene            1437..1937
                     /locus_tag="VNT12_01010"
     CDS             1437..1937
                     /locus_tag="VNT12_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44717.1"
                     /translation="MDDRQAAYRRSWLRAPLGEPAHQPGGKELASDLNLACELYLRGD
                     EALRREIRDSFEEWTAVRGRVLAKAWSFADRLADTCDDRWLRLGLGAVSIDDNGTDFR
                     DTYVALGDLYICAVRCGMDPRPYLEEVAEISSGVPTIERISTRDFLLRFEGSAYFGEA
                     VAPKLK"
     gene            complement(1971..>2709)
                     /locus_tag="VNT12_01015"
     CDS             complement(1971..>2709)
                     /locus_tag="VNT12_01015"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HWI44718.1"
                     /translation="RFVGGHPMAGSQLSGVANASSNLFEGARYFLTPTSRTDPEDYRE
                     VAGFVRELGAVPTAVDPEKHDLLMAALSHLPHLMAAALLKVASDISPEALSFAGPSFR
                     DLTRVGASNPELWSDILAENAPALGEALGAFAGAMAQLGSEIKDRKSMEGRFRQAREA
                     YDALGGILVEKGGANVDVAIPVENRPGVFAEVTTLMGSNNINILDLYVRHSNTARAAL
                     VLTLDAEAAQQARELLRTAGFTAELQG"
BASE COUNT          466 a          788 c          940 g          515 t
ORIGIN      
        1 atgcgggtgc ccgtagcgaa gtcgtccggg gtctgggtgc aaccgtggag catgacgatc
       61 aggggaacgg cttgcccggt gtagccgctc gggatgtaca gtttgtaaga gcgagtcccg
      121 gcgctgttgg tgtacgaacg ctcgacgaag cgtcctctct cggggacgct gccggaggcg
      181 agtcttccag ccttcgtacc cggagggagt tgcacgccgc gggtgagcag ggtcggccca
      241 gcgcgtacat cgcacctcca ccgtcccgaa ggtgatgctt tgagaggaat ccgcgtttct
      301 gagttgttcg atcggcgagg cataagagtg tgtgggagag ttcatacggt tttgtggttg
      361 tgcggtggtt accatatagg tacaggagac gctggcgtat ggaggaggag catgaacgtt
      421 ctcgtgagcg gggctacggg gttcatcggg tcggccctaa ttccggagct cgaggggctc
      481 ggacacaggg tggtccggct gagccgttcg aagccttccg cggaggatac aattcgctgg
      541 gaccccgcct caggtgttct cgatccctcg cgcctgagag ggttggacgc cgtcgtacac
      601 ttggccgcgg agaacgttgg tgatggccgt tggacgccgg ccaagaagag gcgcatcatg
      661 gacagtcgga gagaaggcac ccacctactc gccgagacca tcgccggcct ccctgagccg
      721 cccggagtga tggtctctgt ctcgggcatc aactactacg ggagccgggg aaacgagctc
      781 ttgcgggagg agagtggccc cggaaacagc ttcctcgcca gggtgtgcca ggagtgggag
      841 cgggcggcgg acccggcgcg gagtgccggt gtcagggtcg tgcatcccag gtttggaata
      901 gtgttccccc agggattttc ccgggatctc atgggcatca aggcgggtat cgagcatccc
      961 aagttcgaga ttacgttcgg gacaagaggc tcgacgctcg ccaggttacc tgtctacaag
     1021 cttacggggg gagggtggat cgggtcgggc aggcagttct ggagcttgct cgtcgccgag
     1081 gatgtggtcg gcgtgatcct ccacgccctt accaccgact ccttgcaagg tcctgtcaac
     1141 gcagtgtcga gttccgtcac cgcccgcgaa tacattgtga ccctaaatcg catcttgaat
     1201 cgcccgaccg tcttcccgct tcctgctccc gtagcccgac tggccttggg ggatttcgct
     1261 gaagagcagc tcctggccag catccgtgtc gactccacca agctcacaga ctcaggctac
     1321 gagttccgct acccggagct cgccggcgca ctgcgacatc tagtggagga atagtggctg
     1381 accgaccaag cgaaggtgtt tccgctggag aactctggca agggttgaag atccggatgg
     1441 acgaccgtca ggcggcgtac cgtcgaagct ggctgcgggc gccgctaggg gagcccgctc
     1501 accagccggg agggaaggag ctcgcgtccg accttaacct ggcgtgcgag ttgtacctgc
     1561 ggggcgacga agccctgcgc cgcgagatac gagactcttt cgaggagtgg acggccgtta
     1621 ggggacgcgt gctcgccaaa gcgtggtcgt tcgccgatag gctcgccgac acttgcgacg
     1681 accgctggct gcgtctcggg ctgggtgcgg tctccataga cgacaacggc accgacttca
     1741 gggacaccta cgtggcgcta ggggaccttt atatctgcgc tgtccggtgc ggcatggacc
     1801 cgaggcctta cttggaggag gttgcggaga tctcctccgg cgttccgacc atcgaacgaa
     1861 tttcgacgcg ggatttcctg ctccgtttcg aagggtcggc atacttcggt gaggccgtcg
     1921 cacccaagct aaagtgagga cactcaggtg aggatacggc gctgcccttt tcagccctga
     1981 agctcagcag taaaacccgc ggtccggagt agctctctgg cttgttgagc tgcttcggcg
     2041 tccagggtca gaaccagtgc ggctcttgcg gtgttcgagt gcctcacgta gaggtccagg
     2101 atgttgatgt tgttcgagcc catcagggtg gtcacctcgg cgaagacgcc agggcggttc
     2161 tcgacgggga tggccacgtc cacgttcgcg ccgcccttct cgacgaggat gccgccgagg
     2221 gcgtcgtagg cctcgcgggc ctgcctgaaa cggccctcca tgctcttgcg gtctttgatc
     2281 tcactcccca gctgcgccat cgccccggcg aatgctccca gcgcttcacc gagcgcaggg
     2341 gcgttctctg ccaggatgtc cgaccacagc tccgggttgg aagcgcccac cctggtcagg
     2401 tccctgaaag aggggccggc gaaggagagc gcctcgggag agatgtccga ggcaacctta
     2461 aggagcgcgg ccgccatgag gtggggcagg tgggagagtg ccgccatcag gaggtcgtgc
     2521 ttctccgggt cgacggcggt aggaacggcg ccgagttccc tgacgaagcc ggcgacctcg
     2581 cggtagtcct ccggatcggt gcggctcgtt ggggtgagaa agtagcgggc gccctcgaag
     2641 aggttcgagc ttgcgttggc gacgccagag agctggctgc ccgccatggg gtgtccgccg
     2701 acgaagcgt
//
LOCUS       DATMJA010000043         3290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_480213, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000043 DATMJA010000000
VERSION     DATMJA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480213"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VNT12_01020"
     CDS             <1..307
                     /locus_tag="VNT12_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWI44719.1"
                     /translation="MGVWRVPDGRIDEVGNAFAHYQAVSHCYQRPTYEDWPYSVFSMV
                     HGRSVEECENVLSAMAEETGLTDYTSLYSTREYKKTRVRYFTPEMEAWERLYAGILR"
     gene            363..1103
                     /locus_tag="VNT12_01025"
     CDS             363..1103
                     /locus_tag="VNT12_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI44720.1"
                     /translation="MLSAALLVALFAALLAGLVTGLTGFGLALISTPILLFVYEPRTV
                     IVLTTIFSIVINSAVVWDSWQEARKRLALALLIPALFGIVAGVEVLSVINPDYVRLGV
                     GVIVIFSALLLVRDIRLPGADTRWGTLVAGSASGALSTSTGLAGPPIVLLLASRDLPK
                     HEFRGTSALYFLPMSVVGLAVLAFRGLVEASEIPLGLLLIPAAIAGKAVGTALLRHVS
                     ERAFRVVTLGLVILTGTLGVMTAALALF"
     gene            1166..1960
                     /locus_tag="VNT12_01030"
     CDS             1166..1960
                     /locus_tag="VNT12_01030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWI44721.1"
                     /translation="MTVRVGVQLHPQHASYAQMRDAWLRAEEMGADTIFTWDHFYPLY
                     GDPDGKHFECWTFLGAMAEVTERVEFGALVTCNSYRNPNLLADMARTVDHVSGGRLIL
                     GIGSGWFGKDYDEYGYAFGTAGSRLRDLDAAMPVIEERLGKLNPPPTREIPILIGGGG
                     EKVTLRIVAEHANIWHGFGNPEQAARKSGILDDWCKKVGRDKGEIERSISVRPQLVKD
                     ADRYVEKGITHLIMGFGGPDYDLSPLEDLIAWRDEYRERNGETLAG"
     gene            complement(1999..3159)
                     /locus_tag="VNT12_01035"
     CDS             complement(1999..3159)
                     /locus_tag="VNT12_01035"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HWI44722.1"
                     /translation="MKFERSGFSTRAIHAGQDPDPATGATIVPIYQTSTYTQEAPGQH
                     KGYEYSRTGNPTRTALEECVAALEGARYGLAFASGLAATTAVMSLLSPGDHVVAGDDL
                     YGGTYRLFDKILPRSGGLEFTYVDTTDAASVEVALRSETRLLWIETPTNPMLTLSDIA
                     KLSEMAHGQGAFVAVDNTFASPYFQNPLALGADIVVHSTTKYMGGHSDVIGGAVATSN
                     EEFQEGIKFYQNAAGAVPGVFDSWIVLRGLKTLAVRMRQHEENALAVAEFLQDHPEVE
                     TVHYPGLPDHPQHELAKKQMSGFSGMVSFTLRGGAEAAYAAVQKTRVFHFAESLGGVE
                     SLVTHPATMTHAAIPREQRQARGVTDGLMRLSVGIEDKEDLIADLDGAITAG"
BASE COUNT          548 a         1029 c         1094 g          619 t
ORIGIN      
        1 gatgggggtc tggagagtgc ctgacgggcg catcgacgag gtaggcaacg ccttcgccca
       61 ctaccaggcc gtctcccact gctaccagcg acctacctac gaagactggc cttactccgt
      121 cttctccatg gtccacggcc gcagcgtcga ggagtgcgag aacgtccttt cggcgatggc
      181 cgaggagacg ggacttacag actacacctc cctctattcc acccgcgagt acaagaagac
      241 acgggtgcgc tacttcacgc ccgagatgga ggcctgggag cgcctctacg ccggcattct
      301 gcgctgattc aggtctcagg tatcaggcta caggcgtcag gcttcggggg aggaaccctt
      361 ccttgttgag cgcggcgtta ctcgtggctc tcttcgcggc gttgctcgcc ggactcgtca
      421 ccgggctgac gggcttcgga ctggccctga tcagcacgcc catactcctc ttcgtgtacg
      481 agccccgtac ggtcatcgtg ttgacgacca tcttctcgat cgtcataaac agcgccgtag
      541 tatgggattc ctggcaagag gcgcgcaagc ggctcgcgct ggcgctcctg atccctgccc
      601 tcttcggcat cgtggccggc gtcgaggtgc tgagcgtgat caaccctgac tacgtgcgcc
      661 tcggggttgg cgtgatcgtc atcttctcgg ccctgctcct ggtcagggac atcaggctgc
      721 ctggggccga cactcggtgg gggacactcg tcgccggttc ggccagcggt gcgctctcca
      781 cctcgaccgg gctcgccggc cccccgatag tgttgctcct ggcctcgcgc gacctcccga
      841 agcacgagtt cagggggacg agcgcgctgt acttcttgcc catgagcgtc gtcgggctcg
      901 cggtccttgc cttccgcggc ctggtggaag cctcggagat ccctctgggg ctcctgctca
      961 tcccggccgc catcgcgggt aaggccgtcg gcacggcgct cctcaggcac gtctccgaga
     1021 gggcgttccg ggtggttact ctggggctcg ttatcctgac cggcaccctg ggcgtcatga
     1081 ccgcggcctt agcgctgttt taacctgcct ggtcgcgtcc tccacgtggc ataatcttcc
     1141 ggttggccag aggaggaggc gaaggatgac ggtaagggtc ggggtgcagc tccatccgca
     1201 gcacgccagt tacgcgcaga tgcgcgacgc gtggctcagg gcagaggaga tgggggctga
     1261 cacgattttc acctgggacc acttctaccc gctatacggc gatcccgacg gcaagcactt
     1321 cgagtgctgg acgttcctcg gggcgatggc ggaggtcacg gagagggtag agttcggggc
     1381 gctcgtcacg tgcaactcct acaggaaccc gaacctgctc gcagatatgg cgcgcacggt
     1441 cgaccatgta tcgggcggcc gcctcatact cggtatcggc tcgggctggt tcgggaagga
     1501 ttacgacgag tacggttacg cgttcggaac ggcggggagc cggctgcggg acctcgacgc
     1561 cgctatgcct gttatagagg agcggctagg gaagctgaac cctccgccca cgcgggagat
     1621 ccctatcctc atcggcggtg gaggcgagaa ggttaccctg cgcatcgtcg ccgaacacgc
     1681 caatatctgg cacggtttcg ggaacccgga acaggccgcc cgcaagagcg gcatcctgga
     1741 cgattggtgc aagaaggtcg ggcgcgacaa aggggagata gagcgctcca tatcggtcag
     1801 gccgcaactg gtcaaggacg ccgaccgcta tgtagagaaa ggcatcaccc atctcatcat
     1861 gggcttcggc ggccccgact acgacctgag ccccctggag gacctcatcg cctggcgtga
     1921 cgagtacagg gagcgcaacg gagagaccct agcaggctga tcgccccgag atgggcggcc
     1981 gaaccctccg gaccccgact aaccggctgt tatcgcaccg tccaggtcgg cgatcaggtc
     2041 ctccttgtcc tcgatgccga cggagaggcg catgaggccg tcggtgacac ccctcgcctg
     2101 gcgttgctcg cgcgggattg cggcgtgggt catggtggca ggatgggtta cgagcgactc
     2161 gactccgcct agactttcgg cgaaatggaa gacccgcgtc ttctgcaccg ccgcgtaggc
     2221 agcctccgcg ccgcccctga gggtgaacga gaccatcccc gagaacccgc tcatctgctt
     2281 tttcgccagc tcgtgctggg gatggtcagg caggccgggg tagtgcacgg tctcgacctc
     2341 gggatggtct tgcaagaact cggcgacggc gagggcgttc tcctcgtgct ggcgcatgcg
     2401 gacggcgaga gtcttgaggc cgcggagcac aatccaggag tcgaagacgc ctggaacggc
     2461 gccggcggcg ttctggtaga acttgatccc ctcctggaac tcctcgttcg aggtcgcgac
     2521 agccccgccg atgacgtcag aatggccgcc catgtacttg gtcgtcgagt ggaccacgat
     2581 gtcagcgcca agagcgagcg ggttctggaa gtaaggcgat gcgaaggtgt tgtccaccgc
     2641 gacgaaggcg ccctgcccgt gcgccatttc cgagagcttc gctatgtcgg agagggtgag
     2701 catcgggttg gtcggggtct cgatccatag cagcctcgtc tcagacctta gcgctacttc
     2761 gacggaggca gcatccgtgg tatccacgta ggtgaactca agcccgccgg agcgcgggag
     2821 gatcttatcg aagaggcgat aggtgccgcc gtagaggtcg tctccggcga cgacgtggtc
     2881 ccccggcgag aggagactca tcaccgcggt cgtcgcggcg agccctgagg cgaaggcgag
     2941 gccgtacctg gcgccttcga gggccgccac gcactcttct agggctgtgc gggtcgggtt
     3001 gcccgtcctg ctgtattcat agcccttgtg ctggcccggc gcctcctggg tgtaggtaga
     3061 ggtctggtag atcgggacta tcgtcgcccc tgtcgcggga tctgggtcct gccccgcgtg
     3121 tatggcgcgc gtggagaatc cgctgcgctc gaacttcatg atccttcctc gaggatacgg
     3181 tagaaccttt cgaggtcttc ctgtgtagca tccgagttgg cgagcaccca gtcgtcatcg
     3241 aaggccttgg agacgtagtt gcgcccggtg tctggcagca gtacgacgac
//
LOCUS       DATMJA010000044         3894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_539227, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000044 DATMJA010000000
VERSION     DATMJA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539227"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="hisS"
                     /locus_tag="VNT12_01040"
     CDS             complement(<1..786)
                     /gene="hisS"
                     /locus_tag="VNT12_01040"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWI44723.1"
                     /translation="MTAYRGPRGTYDVFPGGGEPHARPELWTFVEDRARELFDRYNYS
                     EVRTPIFEEARLFARTAGEGSDIVVQKEMFTFTDKGDREMALRPEGTPGVVRAYIEHN
                     LYKLAQPVKLFYIGPMFRQERQQKGRYRQHTQIGVEALGTSDPIVDVEVISLLYAIHQ
                     AVGVKEEVVYVNNLGDMETRQAYVPELRAYLQRHSSQLDPDSVSRMDTKPLRTFDSKH
                     EGTQAVLAEAPRITSYLSSDASAHLAAVRNGLEALGIPYEIDDR"
     gene            complement(783..1358)
                     /locus_tag="VNT12_01045"
     CDS             complement(783..1358)
                     /locus_tag="VNT12_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI44724.1"
                     /translation="MSEIEQVTLSMNGFEVNSYVVHAPECDIVVDAGAEPEKILGATR
                     APVAAILVTHGHADHVGALEPVRRETGAPVYMHPADAEGKVRDYEPLEDGQALDVAGL
                     GIRILHTPGHSPGSVTFVVGHDQIVGDLILPGSVGRTDIQGASWEEIEVSIRRVIPLW
                     EEGTRLYTGHGPVLTAVEELRANPYLPPGLS"
     gene            complement(1370..3595)
                     /locus_tag="VNT12_01050"
     CDS             complement(1370..3595)
                     /locus_tag="VNT12_01050"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWI44725.1"
                     /translation="MEPVRTEERPKAIPAPGVTISSLIEKVSSYGPEGTEELIADAYN
                     VAHAAHRGQTRKSGEPFVYHPLATADVLAELRLDPTTIAAALLHDVLEDTGVTKEELS
                     ERFGEDVAEIVDGVTKLKRLPSGNLEEAQAESLRKMIVAMSRDVRVIIIKLADRLHNM
                     RTLAYLKRETQLKKATETLEIYAPLAHRLGIYSIKWELEDLSFATLHPRRYEEIKSLV
                     AARRGDREAFINGTAAELLRHLRESGIEAEVHGRVKHFYSIYNKMVRRNKEFNEIYDL
                     AGLRVVVDGVRDCYGTLGVIHSIWKPIPGRFKDYIAMPKFNMYQSLHTTVMSNEGKLL
                     EIQIRTSEMDTTAEYGIAAHWMYKHGLTDRQVDRLTWLKSMMEWQKETTDASDFMESL
                     KGELVAEEVFVFTPKGDVVSLPSGATPIDFAYHVHTEVGHRTIGARVNDRIVPLDSEL
                     VSGDRVEVITGKNSGPSRDWLAVVQSGRARNKIRQFFNRADREDNLSLGREKILDLIK
                     KRRIGKIPSGILEDVARATNYTSPEDMFAAVGTGVLSAENVAGRIVEQVQPKEEEKPQ
                     REQSPALAPLPLPDGGTDEQTGVRVVGSSGILTRLARCCTPMPGDEIVGYVSLGRGVV
                     VHSAGCVNASALQARDPERFVEVEWDKGSDKLFTVELLVEALDRMHLLKDITATISDA
                     GVNIVSARVDTIEDRTALSRFAFKSASVQHVEEVIRKIRSIPDVYDVYRVSRDGTPLE
                     H"
     gene            complement(3706..>3894)
                     /locus_tag="VNT12_01055"
     CDS             complement(3706..>3894)
                     /locus_tag="VNT12_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44726.1"
                     /translation="NRLVFAVVTAALFVGSSMLGSFYVAGPQVPVLGAPLVTFLGFTL
                     SIIQTCILLMVIFRSGRL"
BASE COUNT          683 a         1219 c         1192 g          800 t
ORIGIN      
        1 cctgtcgtcg atctcgtaag ggatgccgag cgcctcgagt ccattacgta cggccgcgag
       61 gtgcgccgac gcgtcggaac tcaggtacga ggtgatcctc ggtgcctcag ccagaacggc
      121 ctgggtgccc tcatgcttgg agtcgaaggt ccgcagcggt ttcgtgtcca tgcgggacac
      181 cgagtcgggg tcgagctggc tgctgtgcct ctggaggtac gcgcgtagct cggggacgta
      241 agcctgcctg gtctccatat ccccgaggtt gttgacgtag acgacctcct ccttcacgcc
      301 gactgcctgg tggatggcgt agaggagtga gatcacttcg acgtcgacga tcgggtccga
      361 cgtaccgagg gcctctaccc cgatctgggt gtgctgcctg tagcggccct tctgctggcg
      421 ctcctggcgg aacatggggc cgatgtagaa cagcttgact ggctgtgcca gcttgtacaa
      481 attgtgctcg atgtaggcgc gcaccacacc cggcgtcccc tctggcctta gcgccatctc
      541 ccggtcgccc ttgtccgtga aggtgaacat ctctttctgg acgacgatat ccgagccctc
      601 ccctgctgtc ctggcaaata gcctcgcctc ctcgaagatg ggcgtgcgga cctcggagta
      661 gttgtagcgg tcgaaaagct cccttgccct gtcctcgacg aaggtccaca actcgggtcg
      721 cgcgtgcggt tccccgccac cggggaagac atcgtatgtg cctctcggtc ccctgtaggc
      781 cgtcacgaga ggccgggggg cagataagga ttggctctca gttcctcgac cgccgtgagg
      841 acagggccgt ggcccgtgta gaggcgcgtc ccctcttccc acagcgggat caccctgcgg
      901 atggagacct cgatctcctc ccaagaagcg ccctgaatgt ccgtccggcc gacgctccct
      961 gggaggatga ggtcaccgac gatctgatcg tgtcctacga cgaacgtcac ggagcctggg
     1021 gaatgccccg gcgtatgaag gatcctgatg ccgagccccg cgacatccag tgcctggccg
     1081 tcttcgaggg gctcgtagtc cctgaccttc ccttcggcat ctgcggggtg catgtagaca
     1141 ggggcgcctg tctctctgcg caccggctcc agggcaccca cgtggtcggc gtgaccgtgc
     1201 gtcaccagga tcgccgccac gggagcgcgg gttgcgccga ggatcttctc cggctcggca
     1261 ccggcgtcga cgacgatgtc gcactcaggt gcgtggacca cgtaggagtt gacctcgaag
     1321 ccgttcatag atagtgtgac ctgctcgatc tcgctcaccg gcgagcctcc tagtgctcca
     1381 ggggtgtgcc gtcgcgggag accctgtaga cgtcgtagac gtcggggatg ctccggatct
     1441 tacgtattac ctcctcgacg tgctgcacgc tcgcagactt gaacgcgaac ctgcttagag
     1501 ccgtcctgtc ctcgatggtg tcgaccctgg cagagacgat gttgacgccg gcgtccgaga
     1561 tcgtggccgt tatgtccttc agcagatgca tcctgtcgag ggcttcgacg agcaactcga
     1621 cggtgaagag cttgtcagac cctttgtccc attcgacctc gacaaagcgc tcggggtccc
     1681 tggcctgaag cgcactcgcg ttcacgcagc cggcagaatg aacgacgacg ccgcgtccca
     1741 gtgaaacgta tccgacgatc tcgtcaccag gcatcggggt acaacagcgc gccaggcgcg
     1801 tgaggatccc gctcgacccg acgacgcgca ccccggtctg ctcgtcggtt ccaccatcgg
     1861 ggagcgggag cggggcgagc gcagggctct gctctctctg tggtttctct tcctcctttg
     1921 gctgcacctg ctcgactata cggcctgcca cgttctcggc cgagagcacg cccgtgccga
     1981 cggcggcgaa catgtcctca ggcgatgtgt agttggtcgc gcgggccaca tcctcgagga
     2041 tgccggaggg gatcttaccg atccgccgct tctttataag gtcaaggatc ttctcccgtc
     2101 ccaggctcag gttgtcctca cgatcggccc tgttgaagaa ctgccttatc ttgttccttg
     2161 cgcgcccgct ctggaccacg gccagccagt cgcggctagg ccccgagttc ttgcccgtga
     2221 ttacctcgac cctgtcaccg gagacgagct cggagtcgag ggggacgata cggtcattga
     2281 ccctggcccc tatcgtacgg tggccaacct cggtgtggac gtggtaggcg aagtctatgg
     2341 gtgtcgcccc gctaggaagg ctgaccacat cgcccttcgg cgtgaagacg aagacctcct
     2401 cggcgacgag ctcacccttc aaagactcca taaagtcact ggcgtcggtc gtctctttct
     2461 gccactccat catgctcttg agccaggtca ggcggtccac ctgacgatcc gtaaggccat
     2521 gcttgtacat ccagtgggcc gcgatgccat actccgccgt cgtgtccatc tcggaagtcc
     2581 ggatctgtat ctccagcagc ttcccttcgt ttgacatgac ggtcgtgtga agcgactgat
     2641 acatgttgaa tttgggcatc gctatgtagt ccttgaagcg gccagggata ggcttccaga
     2701 tcgagtgtat gacgccaagg gtgccgtagc agtcgcgcac tccgtcgaca acgaccctga
     2761 ggccggcgag gtcgtagatc tcattgaact ccttgttgcg acggaccatc ttgttgtaga
     2821 tggagtagaa gtgcttgacc ctcccatgga cttcggcctc tatccccgac tcccgcaggt
     2881 gccgcaggag ctcggccgcc gtcccgttga taaacgcctc cctgtccccc cgcctggcgg
     2941 cgaccaggct ctttatctcc tcgtagcgcc tcgggtgtaa ggtggcgaaa gataggtctt
     3001 cgagctccca cttgatggag tagatcccaa gcctgtgggc gaggggcgcg tagatctcca
     3061 gcgtctccgt cgccttcttc agttgggttt cgcgcttgag gtaggcgagg gtcctcatat
     3121 tgtgcagcct gtcagcgagc ttgatgatga tcacacgcac gtcgcggctc atagccacga
     3181 tcatcttcct caaagactcg gcctgcgcct cctccaggtt gcctgaaggc agccgcttga
     3241 gcttggtgac accgtcgact atctcggcca cgtcctcacc gaagcgttcc gacaactcct
     3301 ctttggttac cccggtatcc tcgagcacat cgtgcagcaa cgcagcggcg atggtcgtag
     3361 ggtcgaggcg caactcagcg agaacgtcgg ccgtcgcgag aggatggtag acgaaaggct
     3421 cgccgctctt gcgagtctgg cccctgtgcg ccgcgtgggc cacgttgtag gcgtcggcga
     3481 tcaactcctc cgtcccctcg ggtccgtagg acgatacctt ctcgataaga gaagatatgg
     3541 ttacgccagg cgccggtata gccttcgggc gttcctctgt acgaacgggt tccacagtga
     3601 aattatacca gtaaggcttc aagcatcagg tatcaggtgt tgtatggaac accctgcccg
     3661 cgacgtagcc catcttcgcg caacctgaca cgtgaagcct tacatctata gccttcctga
     3721 cctgaaaatg accatgagga ggatgcaggt ctgaatgatg gagagcgtaa atccgaggaa
     3781 ggtgacgagg ggggcgccga gaacgggcac ctgcgggcct gcgacgtaga aagagccgag
     3841 catggaggac ccgacgaaca gggcggcagt aacgacggcg aagaccagcc ggtt
//
LOCUS       DATMJA010000045         7239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_717106, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000045 DATMJA010000000
VERSION     DATMJA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717106"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VNT12_01060"
     CDS             complement(<1..725)
                     /locus_tag="VNT12_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWI44727.1"
                     /translation="MQKSKARVICNPTSGGGTYDPDEIRDELDGLDLDWIETKRPEDA
                     IQAAQDWREGLLIVAGGDGTVNDVVNGLGRAGFPDGVTLGILPTGTGNDLAATLAVPD
                     DLELAEDVIRQKRVRRLDVARVRSEGVGERCFINVATGGLGAEISNANDEALKKRWGK
                     LSYLRASLEVARNFDVRELSLYLDGEEHRVKAVNIAVGNCRYTGGGWTAAPKANPEDG
                     LLDVVVIETLGAGELLDLAPAALA"
     gene            complement(797..1327)
                     /locus_tag="VNT12_01065"
     CDS             complement(797..1327)
                     /locus_tag="VNT12_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44728.1"
                     /translation="MTETRNNIRQDWLSIPRRREEERLDPHVYKRGINGLTVEEGLRR
                     AFPRVIDILRRSGEDYSEFEQQLVRQTVRACLISQVSRANLYRIAGILVADRRDEGAH
                     IDTSLALAYVAALREAFLTELSVGERVQTPLGPGEVRYVSGDGANAAVKLDTDYEVWL
                     PAERVTRPNPLVRRAS"
     gene            1549..2283
                     /locus_tag="VNT12_01070"
     CDS             1549..2283
                     /locus_tag="VNT12_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWI44729.1"
                     /translation="MNPEEFLQQLKREVIGHHALTHPFLERFGEGDADAEGIRTFAIQ
                     YYRHVRVSRLYLAALISNCRDDEKLQLALAEVLFDEYGHLDPEETHPALYRRFLEALG
                     IGEEEWEEPPTLPEIEMYITTHYALSGHPDFRLGLGALGPASEWPVPPIYARLSDGLR
                     KAAGLPDEALEIFTSHVTMDVTHARIMMEAIAPYAGDEEGQRRVHEGAMRSLDARSVM
                     LGGLYRAVYREPVPLFDAPSVRLTGN"
     gene            2186..3040
                     /locus_tag="VNT12_01075"
     CDS             2186..3040
                     /locus_tag="VNT12_01075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI44730.1"
                     /translation="MPAQLCSAVSTGPSTGNPSLCSTLPPYGLQGTEITSGAPDTPRW
                     YGEDLAYIHDVGHAEFALGAAPGILEILDRNGMHNGRVVDLGCGSGLWARELVDAGYR
                     VLGIDISEAMIELSRNRVPEAEFRVGSLFEVEIPRCQAITAVSEVLNYLFDAENEERG
                     LGRLFRRVHDALVPGGVFVFDVLGPGQVPPGARARGFRVGEDWAVLAEREEDVERGTM
                     ERRIVSFRKAGEHYRRDDEVHRVRLYDPSRLSAELEQAGFRVRTMRSYGAHPLSEGHA
                     AFVARKPA"
     gene            3084..3944
                     /gene="mutM"
                     /locus_tag="VNT12_01080"
     CDS             3084..3944
                     /gene="mutM"
                     /locus_tag="VNT12_01080"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWI44731.1"
                     /translation="MPELPEVETIKEDLRELVAGSEIERALVLDASLVEQPSVEEFAS
                     RLEGVSITGARRRAKHLIMELGSGDSLVFQLKIGGQLLLVPPVEEPETALMLVLDLDG
                     DRRLFLRDETGFTRARLLDADELEERLSSLGPEPLQEEFDAGYLQRAIGSRRAQIKPL
                     LLDQKVVSGIGNIYADEILYDARLHPRRKANTLSEDEWEALHAAIRSNLAAGVEHRGT
                     TVRLYKDVLDRPGEHQNYLRVFEKHGRPCPGCEGEVVRERVSGRPTHFCPACQREDSP
                     GGGGRLESLW"
     gene            3966..5459
                     /locus_tag="VNT12_01085"
     CDS             3966..5459
                     /locus_tag="VNT12_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWI44732.1"
                     /translation="MADQFDLVVIGGGNGGYIPAIRASQLGMSVALIEKRDGGHLGGT
                     CLNLGCIPTKALLQTAHLLHDARNGEDYGVKVGEVEFDYPQAAKRREQVVNQLRRGVQ
                     GLMKKNKVTVYNGVGSFAEPKKIKVETEDGEEELEAENVLISTGSAVNTLPGLEFDHE
                     KIISSDDIVTNDESYPESVIILGSGAVGVEFASMYNDFGTEVTIVEILDRLVPLEDPE
                     ISEALKKEFEGRGITVLTSTKADPESLEKTESGIKIKVAGAESGEQEETEGEGGSYGG
                     EDPPTGEASEASGDTLEAEALLVAVGRKTVIEDLNLDATGVELDERGIIQVDGYGRTA
                     EDGVYAAGDVVGGFWLAHEAGHEGIIAVEHMAGEDPMPKDRNLVPRVTFCRPEVASFG
                     LTKAQAEEEGYEVRETKFPFMAIGKALIEGEPNGFFKVVADEETDLILGMHAIGPKVT
                     DLITEGVFAKLVEGTPEEIGMTMHPHPSLSEVVGEAAMASEGHPIHI"
     gene            5781..6701
                     /gene="lipA"
                     /locus_tag="VNT12_01090"
     CDS             5781..6701
                     /gene="lipA"
                     /locus_tag="VNT12_01090"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWI44733.1"
                     /translation="MPFRQQARGVPGAHGRPEWLKARIPDGEVYRDIKETMRGLNLHT
                     VCEEARCPNIGECWNNRTATFMILGNICTRSCGFCAVLTGKPTELDIDEPYRVADAAK
                     KMGLEHAVITSVNRDELSDGGASIFAATIRAIREEVPGCAVEVLTPDFKGDRDAVKTV
                     IDARPDTFNHNIETVPRLYYAVRPQAKYERSLEVLRYAKELNPDGLTKSGFMVGLGEV
                     EEEIHCTMRDLRDHEVDILTIGQYLRPSENHLPMSRYYTPKEFADLRKYGFSLGFKHV
                     ESGPLVRSSYHAHEQTSDARRSPAAGVASV"
     gene            6813..>7239
                     /gene="lipB"
                     /locus_tag="VNT12_01095"
     CDS             6813..>7239
                     /gene="lipB"
                     /locus_tag="VNT12_01095"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWI44734.1"
                     /translation="MRLRRIEGIPDTFVLCEHPPVYTIGRAAKDAGNLGAGEVYLRAQ
                     GAEVFWSDRGGDATFHGPGQIVGYPILRLAVRDTHAYLRELEGLLIRVLADYGLEGWH
                     HPEYTGVWVGENKIAAIGVKFSSGWITSHGFALNVKTDLS"
BASE COUNT         1429 a         2214 c         2358 g         1238 t
ORIGIN      
        1 gccagggcgg ctggagcgag gtcgagcagt tcccctgccc cgagagtctc tatgacgacc
       61 acgtcgagaa gaccgtcttc gggattggcc ttcggggcgg ctgtccatcc acccccggtg
      121 tagcggcagt tacccacggc aatgttgacg gctttcaccc gatgctcctc accgtcgagg
      181 tagagtgaga gctcgcgcac gtcgaagttc cttgcgacct ccagcgaagc cctgaggtag
      241 gagagcttgc cccaccgctt tttcaacgct tcgtcgttgg cgttggagat ctcagcccca
      301 aggccccccg tcgccacatt gatgaagcaa cgctcgccaa cgccttccga acgcaccctc
      361 gccacgtcta gccgccgcac ccgcttctgc cggatgacgt cctccgcgag ctccagatca
      421 tccgggacgg caagcgtcgc ggcgaggtca ttgcccgtac cggtgggcag gatacccagg
      481 gtgacaccgt cgggaaaacc ggccctgcca agcccgttca ccacgtcgtt tacagtgccg
      541 tctcccccgg cgacgatgag caggccctca cgccaatcct gcgcagcttg tattgcgtcc
      601 tcgggacgtt tagtctcgat ccagtcgaga tccaacccgt cgagctcgtc gcggatctca
      661 tctgggtcgt aggtgccgcc accggaggtc gggttgcaga ttacccgtgc tttcgacttc
      721 tgcatgacgc gccctctgta gcgtttacga ccctcctagt ctacccggaa agcccctggt
      781 tcatgacagg ctatccctag gaagcgcgcc gaaccagcgg attgggtcgc gtcaccctct
      841 cagccgggag ccagacctcg tagtcggtgt ctagcttgac ggcggcgttt gcgccatcac
      901 ccgagacgta gcggacctcg ccgggaccga gcggtgtctg tacgcgctcg ccgacggaga
      961 gctccgtgag gaacgcctcc ctgagcgccg caacgtaggc gagcgccagc gaagtatcga
     1021 tgtgcgcccc ctcgtcgcgc ctgtcggcga ccaggatgcc ggcgatgcgg tagaggttcg
     1081 cccgggagac ctgggagatc agacaagccc gaacagtctg ccttaccaac tgctgctcga
     1141 actcggaata gtcctcgcca ctcctcctca gaatgtctat caccctcgga aacgcccgac
     1201 gtaacccttc ctcgaccgtc agaccgttga tgccgcgctt gtacacgtgc gggtccaacc
     1261 gctcctcctc ccgccgccgc gggatactga gccaatcctg cctgatgttg ttcctcgtct
     1321 ctgtcatgca cccattatac ctcactgaga ctagtgagac aaggacgaat tacctggaaa
     1381 gtgagcattt cagcacgcta cgctctttgg gcttcgcttg tcagcaagct ctggcttcgc
     1441 ctccgcttgt cagcacggcg gctgcgccgc cttctcagcc agtcagccag agcttgggca
     1501 cgcgtacctt gcgctaaact atagggagtt ctctggaagg agcgacgggt gaacccggag
     1561 gagtttcttc agcagctaaa gcgcgaggtc atcgggcatc atgcgctcac gcacccattt
     1621 ctggagcggt ttggggaggg ggatgctgat gcggagggga taaggacgtt tgcgatccag
     1681 tactacaggc acgtcagggt gagccggttg tacctggccg cccttatctc gaactgccgc
     1741 gacgatgaga agctccagct cgcactcgcc gaggttctct tcgatgagta cggtcacctc
     1801 gaccccgaag agacgcatcc agccctgtac aggcgtttcc tggaggcgct tggcatcggt
     1861 gaagaggagt gggaggaacc tccgacgctg cccgagatag agatgtacat caccacccac
     1921 tacgcgctct cgggccatcc agacttcagg ttgggtctcg gtgctctggg gccggccagc
     1981 gagtggccgg tgcccccgat ctacgcccgg ctctccgacg gcctcagaaa ggctgccggc
     2041 ctgcctgatg aggcgctgga gatcttcacc agccacgtca cgatggacgt gacccacgcc
     2101 cgcatcatga tggaggccat cgcgccctac gcaggggacg aggaaggcca acgaagagtc
     2161 catgagggcg ccatgcgctc gctcgatgcc cgctcagtta tgctcggcgg tctctacagg
     2221 gccgtctaca gggaacccgt ccctctgttc gacgctccct ccgtacggct tacagggaac
     2281 tgagataacc tcgggagccc ctgacacgcc gcggtggtat ggggaggatc tcgcgtacat
     2341 ccacgacgtc ggccacgccg agttcgccct cggagcggcg ccgggcatac tggagatcct
     2401 ggaccgtaac gggatgcaca atggccgcgt tgtcgacctc ggttgcggta gcggtttgtg
     2461 ggcgcgcgag ctcgtagacg ccggctacag ggtgttgggg atcgacatct ctgaggctat
     2521 gatcgaactc tcgcgcaata gagtgcccga agccgagttc agggtagggt cgctgttcga
     2581 ggtcgagatc ccacgttgtc aggccatcac ggccgtcagc gaggtcctca actacctttt
     2641 cgatgcggag aacgaggaac gggggcttgg tcgcctattc cggcgcgtgc acgacgcgct
     2701 cgtccccggc ggcgtcttcg tcttcgacgt gcttggtcct gggcaggtgc cgcctggtgc
     2761 cagggcgaga ggtttccgcg tgggggagga ctgggcggta ctcgcagagc gggaggagga
     2821 cgtcgagcgg ggtacgatgg agcgccggat cgtgagtttc cgcaaggcgg gggagcatta
     2881 cagacgggat gacgaggtgc accgcgtgcg gctctacgac ccgtcgaggt tgtccgcaga
     2941 actcgaacag gcgggcttcc gggttcggac gatgcgatcc tacggtgctc acccgctatc
     3001 ggagggccac gccgccttcg tcgcgcgcaa accggcatga ggcagcaaga ataggtcagc
     3061 gccgagcggg tataaatacg cgtatgccgg agttgccaga ggtcgagacc ataaaggaag
     3121 acctgcgcga gctggtcgcc ggctcggaga tagagcgggc actagtcctc gatgcttcgc
     3181 tcgtggagca gccttcggtg gaagagttcg caagccggct cgaaggtgtc agcatcacag
     3241 gggcgcggcg gcgggccaag cacctgatca tggagcttgg ctcaggagac tcgctggtgt
     3301 ttcaactgaa gatcgggggc cagctcctgc tcgtgccacc ggtcgaggaa ccagagaccg
     3361 cgctcatgct cgtcctcgat ctcgacggtg acaggaggct cttcctgcgc gacgagacgg
     3421 gcttcacgcg ggcgcgcctg ctcgacgcgg acgaactcga ggagcggctc tcctcgctcg
     3481 gaccagagcc cctacaggaa gagttcgatg cgggatattt gcaacgggcc atcggttcgc
     3541 ggagggccca gataaagccc ctgctcctgg atcagaaagt cgtctcaggc atagggaaca
     3601 tctacgccga tgagatcctg tatgatgcga ggctgcatcc gaggcgcaag gcgaacacgc
     3661 tctcggagga cgaatgggag gcgctgcacg cggcgatacg gtccaacctc gctgccggcg
     3721 tggagcatcg gggcaccact gtgcggctct acaaggatgt attggaccgg ccaggcgagc
     3781 atcagaacta cctgcgggtc ttcgagaagc acgggcggcc gtgtccgggg tgtgaaggag
     3841 aggtggttcg ggaaagggta agcggtaggc cgacgcactt ctgtccagcg tgccagcggg
     3901 aggatagccc cggaggaggg ggaaggttag aatcgttgtg gtagtagacg cgaaaggagc
     3961 caagggtggc tgatcagttc gacctcgtgg taatcggcgg cgggaacggt ggatacatac
     4021 cggcgatccg ggccagccag ctggggatga gcgtagccct catcgagaag cgggatggcg
     4081 ggcatctcgg agggacttgc ctgaacttgg gctgtatccc gaccaaagct ttgctccaga
     4141 cggcccatct attgcacgac gccagaaacg gcgaagacta cggggtcaag gtcggcgagg
     4201 tggagttcga ctacccgcag gcggccaagc gccgcgaaca ggtcgtcaac cagctcaggc
     4261 gcggtgtgca gggcctgatg aagaagaaca aggtcaccgt ctacaacggt gtaggcagct
     4321 tcgccgaacc caagaagata aaggtagaga ccgaagacgg cgaggaggag ctcgaagcgg
     4381 agaacgtcct gatctccacc gggtcggccg tcaacaccct gcccggcctg gagttcgacc
     4441 atgagaagat catctcctca gacgacatcg tcaccaacga cgagagctac ccggagtccg
     4501 tgatcatcct cggctcgggt gccgtcggcg tcgagttcgc gagcatgtac aacgatttcg
     4561 gcaccgaggt gaccatcgtc gagatcctgg accgtctcgt gccgctggag gacccggaga
     4621 tctccgaggc gctgaagaag gagttcgagg gccgcggcat cacggtgctc acgagcacca
     4681 aagccgaccc tgagagcctg gagaagaccg aaagtggtat caagatcaag gtggcaggcg
     4741 cggaatcagg cgagcaggag gagacggaag gcgaaggcgg ctcctacggc ggcgaagacc
     4801 cgcccaccgg cgaggccagc gaagctagtg gcgacaccct ggaggccgag gcgttgctcg
     4861 tggcagtcgg aaggaagacc gttatcgagg acctcaacct cgacgcgacc ggcgtcgagc
     4921 tcgatgagcg cggcatcatc caggtggacg ggtacgggcg gacggccgag gacggcgtgt
     4981 acgcggcagg ggatgtagtc gggggtttct ggctggccca cgaggccgga cacgagggca
     5041 tcatcgccgt cgagcacatg gccggcgagg acccgatgcc caaggacagg aacctcgtcc
     5101 cgcgcgtcac cttctgtagg cccgaggtgg cctctttcgg gctcaccaag gcacaggcag
     5161 aggaggaagg ttacgaggtc agggagacga agtttccgtt catggctata ggcaaggcgc
     5221 ttatcgaggg cgagcccaac gggttcttca aagtcgtcgc cgacgaggag acagatctga
     5281 tcctcgggat gcacgccata gggccgaagg tcacggatct catcaccgaa ggcgttttcg
     5341 ccaagctcgt cgagggcacc ccagaggaga tcggcatgac catgcatccc cacccgagcc
     5401 tctcggaggt cgtcggggaa gcggcgatgg cctcggaagg acacccgatt catatatagg
     5461 tttcaggtat cagccgtcag gtgggttttg gggtcacgta gcaacgtggc gccaacgtcg
     5521 ctctcgtgca cttcctgaga tctgtagccc gcaatgggaa ggcccgaagc cttcccattg
     5581 cgcactgaga taggtgacac ggcacggcac ggcctccgtg ctataccata ggcgcagaat
     5641 gcgaagaaga taggagcgct gtggcccgca gacagataga gctgaaggta ttggacgacg
     5701 gtacgaagac cttcgaggtc cgacaccgct gggaagacag gcccgtgccc ccgccgcccg
     5761 acgggacgcc gacggagtac ctgcccttcc ggcagcaggc caggggtgta ccgggcgcgc
     5821 acggtcgtcc cgaatggcta aaggcgcgca tcccagacgg cgaggtctac cgcgacatca
     5881 aggagaccat gcgcggcctg aacctacata cggtctgcga ggaggcccgc tgcccgaaca
     5941 tcggtgagtg ttggaacaac cgcaccgcca ccttcatgat cctcggcaac atctgtaccc
     6001 gttcctgcgg cttctgcgcc gtgctaacgg gcaagcccac cgagcttgac atcgacgagc
     6061 cctaccgcgt cgccgatgcc gcaaagaaga tgggcctgga gcacgctgtg atcacgagcg
     6121 tgaatcgcga cgaactctca gacggcgggg cctccatctt cgccgcaacg atacgcgcga
     6181 tcagggaaga ggtccccggc tgcgccgtcg aagtcctgac tcccgacttc aagggagatc
     6241 gcgacgcggt caagaccgtc attgatgccc gtcctgacac cttcaaccac aacatcgaga
     6301 ctgtcccccg cctctactat gcggtccgtc cgcaggcgaa gtacgagcgc tccctggagg
     6361 tgctgcgcta cgccaaggag ctgaaccctg acggcctgac caagagcggc tttatggtcg
     6421 gtctcggcga agtcgaagag gagatccact gtacgatgcg cgacctgcgc gatcacgagg
     6481 tggacatcct gaccatcggg cagtatctca ggccctctga gaaccacctg ccgatgagcc
     6541 gctactacac gcccaaagag ttcgccgacc tccggaagta cggcttctca ctgggtttca
     6601 aacatgtcga aagcggcccg ctcgtgcgca gctcctacca cgcccacgaa cagacctcag
     6661 acgccaggcg aagccctgcg gcaggcgtcg cgagcgtcta gcgtcagggc atgaccctgg
     6721 agaagcagac taggtccgaa acgaccgtcg atgtgcgtcg gctgcctgga ttgacgcctt
     6781 acgctgaggc ctgggatctc cagaaggatc tcgtgcggct caggaggatc gaagggatcc
     6841 ccgacacgtt cgtcctgtgc gagcacccgc cggtctacac aataggaagg gcggccaagg
     6901 acgcggggaa cctgggcgcc ggagaagtgt acctcagggc tcagggggcc gaggttttct
     6961 ggagcgatag gggaggggat gcaaccttcc acggtcctgg tcagatcgtg gggtacccga
     7021 tcctgcgcct cgcggtgcgt gacacccacg cctacctccg cgaactcgaa ggactcctca
     7081 tccgggtgct cgcagactac gggctcgagg gctggcacca tccagagtac acgggtgtgt
     7141 gggtagggga gaacaagatt gccgccatcg gcgtgaagtt ctcgtctggt tggatcacct
     7201 cccacggctt cgccctgaac gtgaagacag acctgagct
//
LOCUS       DATMJA010000046         3152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_362183, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000046 DATMJA010000000
VERSION     DATMJA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362183"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VNT12_01100"
     CDS             complement(<1..1122)
                     /locus_tag="VNT12_01100"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWI44735.1"
                     /translation="MRRVEIFDTTLRDGEQSPGISLSVEEKVEIARQLARLKVDVIEA
                     GFPITSEGDFESVSRIASEVKGPRITGLARIHEQDIVRAWEAVQWSHRPRIHTFVGTS
                     DLHIEHQMRSNREDILRRAGEAVAFAKSLCENVEFSPMDATRTDIGYLSEIVAAAVEA
                     GADVVNIADTVGYTTPVEFADFLRELQERVPALQERVLSVHCHDDLGMAVANSLAGVE
                     VGAGQVEVAVNGIGERAGNASLEEVVMALATRSDFYGATVGVVTRQLANTSRMVSNIT
                     GYEVPPNKAIVGRNAFLHESGIHQDGVLKDRRTFEIMTKEDIGLEGTNIFLGKHSGRH
                     ALKDALEELGYYVEGDVLKRAFTRFKEIADHKKTVTAADL"
     gene            complement(1306..2262)
                     /locus_tag="VNT12_01105"
     CDS             complement(1306..2262)
                     /locus_tag="VNT12_01105"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWI44736.1"
                     /translation="MSRKAFAASDAEWAYHGGELVRVGDIRLSPATHALNYGTGVFEG
                     IRAYWSERRGTLQVLKMREHYERFERSCRLLRIELPNTVDELCDVTLEILSRNAPRED
                     TYVRPLAYKAAESVGVNLKGETELSIFTVPMGNYVELTGLKVCVSSWRRTPDNAIPAR
                     AKCTGSYVNTALAVDEAHMAGYDDAIFLTQDGQVSEASAANIFLVRKGQLITPPVTAD
                     ILEGITRDAVMELAGKELGMPVIEREVGRTELYAADEVFLSGTGFQIAPVVEVDDRPV
                     GTGSIGPVAERLQELYFKAARGEWEEYAGWTVAVEVAERAAG"
     gene            complement(2259..>3152)
                     /gene="ilvC"
                     /locus_tag="VNT12_01110"
     CDS             complement(2259..>3152)
                     /gene="ilvC"
                     /locus_tag="VNT12_01110"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWI44737.1"
                     /translation="VGLYEGSKSWSKAEGDGLQVKTIADAVRESDVAMMLLPDEKQPA
                     VFETEVGPNLSEGDLMLFAHGFNIHFNQIVVPPEVDLGLVAPKGPGHVLRRLYIEGKG
                     MPSLFAVEHDASGQARDVILSYARAIGSGRAGIIETTFAEETETDLFGEQAVLCGGLT
                     ALLTAGFETLVEAGYQPELAYYECVNELKLIVDLIYEGGLAGMRYSISNTAEYGDYTA
                     GPKIIDEAARERMRSILADIQSGKWAKDWVLENQAGGSSFLAMRRREAESQVEKVGQE
                     LRALAAEGIETPAGSAGGENL"
BASE COUNT          512 a         1043 c          974 g          623 t
ORIGIN      
        1 caggtctgct gctgttacgg tcttcttgtg gtccgctatc tccttgaaac gtgtgaaggc
       61 ccgtttcagc acatcgccct ccacgtagta gccgagctct tcgagggcgt ccttgagtgc
      121 gtggcgcccc gagtgcttac cgaggaagat gttggttcct tccaggccga tgtcttcttt
      181 cgtcatgatc tcgaacgtcc gcctgtcctt gagcacgccg tcctggtgga tgcccgactc
      241 gtgcagaaag gcgttgcgcc ccacgatggc cttgttcggg ggaacctcgt agcccgttat
      301 gttggagacc atgcggctcg tgttggcgag ctggcgggtc acgacgccga ccgtggcgcc
      361 gtagaagtca gagcgcgtgg cgagcgccat tacgacctcc tccagcgagg cattccctgc
      421 ccgctccccg atgccgttga cggccacctc gacctggccg gccccaacct cgacccccgc
      481 gagcgagttg gcgacggcca tcccgaggtc gtcgtggcag tggaccgaga ggacacgctc
      541 ctggagggca ggcacccgct cttgtagctc tctcagaaag tccgcgaact cgaccggcgt
      601 ggtgtagcct accgtgtcgg cgatgttgac cacgtcggcc cctgcttcga cggccgcggc
      661 gacgatctcg gagagatagc ctatgtccgt gcgcgtcgcg tccatcggtg agaactcgac
      721 gttctcgcag aggctcttcg cgaaggccac ggcctcgccg gccctccgca ggatgtcttc
      781 ccggttggag cgcatctggt gctctatgtg caggtcgctg gtgccgacga aggtgtggat
      841 gcgtggcctg tgtgaccact gcacagcctc ccaggcgcgc acgatgtcct gctcgtggat
      901 gcgggcgagg cctgtgatcc tgggcccctt tacctccgag gcgatgcggc tcaccgactc
      961 gaagtctccc tcggaggtga tggggaaccc ggcctctatt acgtccacct tcaggcgagc
     1021 gagctggcgg gctatctcta ccttctcctc tacggacagg gagatccctg gcgactgctc
     1081 gccgtcgcgg agtgtggtgt cgaagatctc tactcgacgc atgtgtctcg tcctcctacg
     1141 tttggaaacc ttttgaagaa atgcgacttg aggctggtgg ccgcggacag ccggctagaa
     1201 accggggccg gtaattcgcc cggcggccac cctggttagt cgtaggctgt ggcgagaaac
     1261 acgcaagatg tctagaggtt ctccttcatg aagggcggca gtatctcatc ccgccgcccg
     1321 ctccgcaacc tcgacggcca cggtccagcc ggcgtactct tcccactcgc cgcgcgcggc
     1381 cttgaagtac agctcctgca ggcgctcggc gacgggtcct atgctgccgg tcccgacggg
     1441 acggtcgtcc acctcgacga cgggtgcgat ctggaacccc gtcccagaga ggaacacctc
     1501 gtctgcggcg tagagctccg tacgtcccac ctcgcgctct atgacgggca tcccgagctc
     1561 cttgccggcc agctccatga cggcgtcgcg cgttatgccc tccaggatgt cagccgtaac
     1621 gggcggtgtg atgagctggc ctttcctgac caggaagatg ttcgccgcgc tagcctcgga
     1681 gacctggccg tcctgggtga ggaagatggc gtcgtcatag cctgccatgt gcgcctcatc
     1741 gacggcgagc gcagtgttga cgtaggagcc cgtgcacttg gcgcgggccg ggatagcgtt
     1801 atccggcgtg cgccgccagg aagagacaca aaccttcagt cccgtaagct ccacatagtt
     1861 gcccatgggg accgtgaaga tagatagttc ggtctcaccc ttcagattca cccccacgga
     1921 ctcggcggcc ttgtaagcca ggggccgaac gtaggtgtcc tcgcgcgggg cgttgcggct
     1981 caggatctcc aatgtgacgt cacagagttc gtcgacggta ttgggcaact ctatacgtag
     2041 cagccggcag gatctctcga agcgctcgta gtgctcgcgc atcttcagga cctgcaacgt
     2101 cccgcgcctc tcactccagt aggcccttat gccctcgaaa acaccggtgc cgtagttcag
     2161 ggcgtgcgtc gccggtgaga ggcgaatgtc gccgaccctc accagctcgc cgccgtggta
     2221 ggcccactcg gcgtctgacg cagcgaacgc ttttcgactc ataggttctc acctccagcg
     2281 gagccagcgg gcgtctcgat accctcggcc gcgagcgccc gcagctcctg tccgaccttc
     2341 tccacctgcg actctgcctc cctgcggcgc atcgcgagga agctcgatcc cccggcctgg
     2401 ttctcgagca cccagtcttt ggcccacttg ccgctctgga tatccgcgag gatagaacgc
     2461 atcctctctc ttgcggcctc gtcgatgatc ttcggccccg ccgtgtagtc gccgtactcg
     2521 gcggtgttcg agatcgagta gcgcatcccg gcaagcccgc cctcgtagat caggtcgacg
     2581 atgagcttga gctcgtttac gcactcgtaa taggcgagct ctggctggta gccagcctcg
     2641 acgagcgtct cgaacccggc cgtaagaagg gccgtcagcc ctccacacaa caccgcctgc
     2701 tccccaaaga ggtcagtctc ggtctcctcc gcgaacgtgg tctcgataat gccggcgcgg
     2761 ccgctaccta tggcgcgggc gtaggagagg atcacatccc gagcctgacc cgaagcgtcg
     2821 tgctcgaccg caaagagcga aggcatccct ttgccctcga tgtacaggcg cctgagcacg
     2881 tgaccgggtc ccttgggggc gacgagaccc aagtcgacct cgggggggac gacgatctgg
     2941 ttgaagtgga tgttgaagcc gtgggcgaag agcatgaggt cgccctctga gaggttcggc
     3001 ccgacctcgg tctcgaagac ggcgggctgc ttctcatcgg gcaggagcat catcgccacg
     3061 tcggattctc tcaccgcgtc agcgatggtc ttcacctgca ggccgtcccc ttctgctttg
     3121 gaccagctct tcgagccctc gtagagcccg ac
//
LOCUS       DATMJA010000047         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_480578, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000047 DATMJA010000000
VERSION     DATMJA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480578"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VNT12_01115"
     CDS             <1..242
                     /locus_tag="VNT12_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44738.1"
                     /translation="TDGREGRAAMVVCIGSPDEEDLVRGRRCLRKAGIPVEHFGIAAA
                     GLVFLVSADVGLEALRALHAGLIPPAGDFAGEEVA"
     gene            239..1303
                     /locus_tag="VNT12_01120"
     CDS             239..1303
                     /locus_tag="VNT12_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44739.1"
                     /translation="MRRLEIVQLGLGHVGRAVAQIVLEERKRWLERHGIEVRYRAVSD
                     TSGALVGEELLPQALRLKEDGGRLAELGIEPLEGVLASEPEPGFTRVVVDVAVHGGTF
                     DLDVLGIKGGAYLVLSNKGPLSGSTAQYRQLMRMIPDRLWHEATVGAGMPIISTIGGL
                     LETGDEILEIRASPSGTLGFVMSAVEGGRSFSEAVKEAVDLHYAEPDPRDDLSGLDVA
                     RKAIILARMMGREIEPHEIPYESLVPEGLEDVPLTNFMERLPEHDEAFAARLLGVEKH
                     HMLRYLVCIPKHGPVTVGLQDTPVESPFGPISGPENVFDFRTRRYSDVTLTVRGPGAG
                     PQRTASGVVWDLLDIAHKAL"
     gene            1340..2302
                     /locus_tag="VNT12_01125"
     CDS             1340..2302
                     /locus_tag="VNT12_01125"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWI44740.1"
                     /translation="MSDRYTVAIVGAGLVGDRLVAELRRRDFPLRELRILARSARTAV
                     LAGETFEVRVAEPEAFEGVDIAFFAGTEGEKGAAVQLAGEAISRGAVVIDNGSDFRHD
                     PNVPLVVPEVNVGALEDHEGLIANANCSTTQMVVTLAPLAREFGLKKVIVSTYQAVSG
                     AGRSGVEALENGREDAFPRPIVGNAIPQIGGVGDDGYTTEETKMREETRKILSLPDLP
                     VFATAVRIPVHTGHAESVFVETEREADLPGVLEALGAAPGVEVSDGFPTPLEVAGEDG
                     TYVGRVRVDGRVIQYWCVADNLLKGAATNAVQIAESLLGVHARA"
     gene            2406..2678
                     /locus_tag="VNT12_01130"
     CDS             2406..2678
                     /locus_tag="VNT12_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44741.1"
                     /translation="MAQGEKRSFWDRLFGRNQPSALEERVVEYIIHRLGEGAHLGDVV
                     KEEYVRRNASPLEIEEICADPRLVEAARQHLRQDFDSGELDPDKRP"
BASE COUNT          503 a          823 c          945 g          444 t
ORIGIN      
        1 gcacggatgg ccgcgaggga agagccgcga tggtcgtctg catcggctcg ccggatgaag
       61 aggatcttgt ccgcggccgg cgttgcctgc gcaaggcggg tatccccgta gagcacttcg
      121 gcatcgcggc cgctgggctc gtgttcctgg tgtccgccga cgttggcctc gaggcgctgc
      181 gagcgctgca cgcgggcctg atcccaccag caggagactt cgccggcgaa gaggtagcgt
      241 gaggcggctg gagatagtcc agcttggcct gggacacgtc ggccgggcgg tcgcccagat
      301 agtcctggaa gagcgcaaac gctggctcga acgtcacggt atcgaggtga ggtaccgcgc
      361 cgtctcggat acctcgggcg cgctggtcgg tgaggagttg ctaccgcagg cgctacgcct
      421 caaggaggat ggaggcaggc tcgccgagct tggcatcgag ccactggaag gcgtgctcgc
      481 ctcggagccc gagcccggct tcacacgggt agtcgtagac gtggcagtgc acggcggtac
      541 cttcgacctc gatgtgctcg gtataaaggg cggggcttat ctcgtgctct ccaacaaagg
      601 accactctcg ggcagcacgg cccagtaccg ccagctcatg cgaatgatcc cagaccgtct
      661 ctggcacgag gccacggtgg gtgctggcat gcccatcatc tcgaccatag gcggcctcct
      721 ggagacgggg gacgagatac tggagatacg ggcgagcccg agcggcaccc tcggcttcgt
      781 catgagcgcg gtcgagggcg gccggtcctt ctccgaagcg gtaaaggagg cggtcgatct
      841 ccactacgcc gaacccgacc ccagggacga cctctcgggg ctcgatgtgg cccgcaaagc
      901 catcatcctg gcgcgaatga tgggaaggga gatcgaacca catgagatcc cctacgagtc
      961 actggtcccg gaaggcctgg aagacgtccc gttgacgaac ttcatggagc gtctccccga
     1021 acacgacgag gctttcgccg cgcgccttct tggtgtcgaa aaacaccaca tgctccgcta
     1081 cctggtctgt atcccgaagc atgggcccgt cacggtcggg ctgcaggata cgcccgtgga
     1141 gagccccttc ggacccataa gcggccccga gaacgtcttc gacttccgca cgcgccgcta
     1201 ctcggatgtt actctcaccg tgcgcgggcc aggcgcggga ccacaacgca cggcgagtgg
     1261 cgtggtctgg gacctgctgg acatcgcgca caaggctttg taggtaacag ggttcaggta
     1321 gtctctggtg gagggtctta tgagtgatag gtatacggta gcgatagtag gggccgggct
     1381 cgttggggac aggctggtcg ccgagctgag gcgcagagac ttcccgctta gagagttgcg
     1441 catcctggcc aggagcgcga gaaccgcggt cctcgcgggg gagacgttcg aggtcagggt
     1501 ggccgagcca gaggctttcg aaggcgtgga catcgccttc ttcgccggga cggaagggga
     1561 gaagggggcc gccgtccagc tcgccggtga ggctatctcg cgcggcgccg tcgtcatcga
     1621 caacggcagc gacttcaggc acgacccgaa tgtgcccctc gtcgtccccg aggtaaacgt
     1681 gggtgcgctc gaggaccacg aaggcctcat cgccaacgcc aactgctcca ccacccagat
     1741 ggtcgtcaca ctcgcgccgc tcgcgcgcga gttcgggctg aagaaggtga tcgtctcgac
     1801 ctaccaggcg gtctcgggcg cgggacgcag cggcgtcgag gcgttggaga acggcaggga
     1861 ggacgcgttc ccaaggccca tagtggggaa cgccataccc cagatcgggg gtgtcggcga
     1921 cgacggatac acgaccgagg agaccaagat gagggaggag acacgcaaga ttctctctct
     1981 acccgacctt cccgttttcg ctaccgccgt caggattccg gtccacacgg gccacgccga
     2041 gagcgtcttc gtcgagaccg agcgggaggc agatctcccc ggggtgctcg aagcgctagg
     2101 ggcggcgcct ggcgtcgagg tctcggacgg cttcccgacg ccgctcgagg tcgccggaga
     2161 ggatgggacc tacgtcgggc gcgtacgagt ggatggacga gtgatccaat actggtgcgt
     2221 cgcggacaac ctcctcaagg gcgcggcgac gaacgccgtc cagatcgccg aatcgctcct
     2281 cggcgttcac gcgcgggcgt agaggcccgt tctggaggcg cggttcggcc cttgccaccc
     2341 tgtacggaga acccgcaact cgttaaactt cggcgtggga agatacccgc ggtgaggagt
     2401 gaggcatggc acaaggcgaa aagagatcct tctgggacag attgtttggc cgaaatcagc
     2461 cttctgcgct ggaggagagg gtggtcgagt acattatcca ccgcctgggc gaaggggcgc
     2521 acctgggtga tgtggtaaag gaggagtacg tgcggcgcaa cgcttcaccc ctcgagatag
     2581 aggagatctg cgccgatccc aggctcgtcg aagccgcccg ccagcacttg aggcaggact
     2641 tcgactcggg ggaactcgac cctgacaagc gtccatgata gccgcctggt gcggagggga
     2701 ccggaagtct atcta
//
LOCUS       DATMJA010000048         6405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_481004, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000048 DATMJA010000000
VERSION     DATMJA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481004"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VNT12_01135"
     CDS             <1..432
                     /locus_tag="VNT12_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HWI44742.1"
                     /translation="ALAKTATSALEAEKMGFLTENDRTVMNADHLISAAKREALDLAD
                     GYTPPEHANNVYAAGRTARAALEIGIKTMQWGHYASEYDGVITGHVARILTGGNLSLP
                     QWVPEEYLLKLEKKAFLDLLKQEKTHERIEALLKTGKPVRN"
     gene            529..1707
                     /locus_tag="VNT12_01140"
     CDS             529..1707
                     /locus_tag="VNT12_01140"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWI44743.1"
                     /translation="MKEAVIVSGARTAVGRAPRGTLRASRPDDMAAAAIGEAVRRAEG
                     LEREDVEDVIIGCAMPEGTQGYNVARLSSLLAGMPDTVPAQTVNRFCSSGLQTIAMAS
                     ERIIIGNATTIVAGGTEHMSSTVEMPNFSPNPRLMEINPAAYMGMGLTAEQVAREFDV
                     SREDQDEFALRSHTLAKEAVEGGVFDEEIVPLAVEFDWVDESGEAQHMETSFGRDEGP
                     RDTSAEKLANLKPAFQQDGTVTAGNSSQRSDGAAAVVVMDREEAERRGLAPLVRFVGF
                     AVGGVRPEVMGIGPTVAIPKVLEQTGLTMDDIDLIEFNEAFAAQALAVIREVGMDLEK
                     TNVNGGAIALGHPMGATGTKLTVQLIHEMKRRGSKYGLVTMCIGGGMGAAGIFENLQN
                     "
     gene            complement(1930..3273)
                     /locus_tag="VNT12_01145"
     CDS             complement(1930..3273)
                     /locus_tag="VNT12_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44744.1"
                     /translation="MRAHLDALRPVLGIVFLAGYAWQTFAPDSFGTDLFLGVGAVLFV
                     ISVPWSSAFHRAFALAALVALGVVIFTGRFDLGDFLQGMPTYFGIVAVLLVLSMAGYP
                     IQAARYEVQIRALMAALTRRGVSIKATAGVLGHILGAVLDVGAFVLIDVISGRSAPKE
                     RLEALKWAGRTFSFAPLWTNLNVLTATTIVLTGVSYPMLLAVTLPFVLIGLGGTLLFA
                     QRAGGEVEEIPQAPLNRGAAAVLLYPVVLVTAVALVNLSFPALPLTAAIAVTIATIVL
                     SIAGLAAVLTLGASPLVRLGQEARGSLTASHSEFALFGAAGILVLSLKALGALAPLGA
                     LLSAVPTVLVAPALALIMGVGFVAGIHVIPMALLLNTAFPLDGGPAPALWAAALLLGA
                     QSTLLLTPFSSAVTMLSRLTGLHPLEIGPRRNLGFCLIMAVAAMLYLAILTFVLL"
     gene            3511..4692
                     /locus_tag="VNT12_01150"
     CDS             3511..4692
                     /locus_tag="VNT12_01150"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWI44745.1"
                     /translation="MQGSARMGGSFGKTVLLVVLLVLMCAACGGSAEERGKSDNDIEP
                     ITTGQGEKEEDTSNQGGASVEQVEVSTLATDLDVPWSFAFLPGGDALVTERDSGRLLR
                     VSRSGDVQEIQTLPEGGSGEGGLLGVAVSPDYEDDRYIYAYYTTGVDNRVVRFRKGES
                     LEPILTGIPSNTYHDGGRIKFGPDGMLYVSTGDAGESGNSQNRNSLGGKILRIEPDGS
                     IPPDNPFPGNPVYSYGHRNVEGLAWDSEGQLYASEFGASTWDEVNRVEAGENYGWPAV
                     EGRGGEDQGYVDPITVWPTSEASPSGAEIMVEGAVPQWEGDLFVAALRGERLWRLELN
                     DSGEVVDREKLLDGEVGRVRDVIQAPDGSLWVSTSNHDSYASPVSEQDDRILRLAPAR
                     E"
     gene            complement(4830..5153)
                     /locus_tag="VNT12_01155"
     CDS             complement(4830..5153)
                     /locus_tag="VNT12_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HWI44746.1"
                     /translation="MTDTIARPVEKEEQKTEGLPPYNVVLLDDNDHSYEYVIFMLKKV
                     FGHPVEKGYEMAREVDATGRVVVATTNLEQAELKRDQIQAFGPDPLIPRCKGSMSATV
                     EPASG"
     gene            complement(5208..5999)
                     /locus_tag="VNT12_01160"
     CDS             complement(5208..5999)
                     /locus_tag="VNT12_01160"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWI44747.1"
                     /translation="MIEVEKTYEHILFEQDGAVAYVTMNRPKKRNALSLEHMRELISL
                     FEEIGERKDASVVILRGEGPAFCAGHDLSEMIGRDPGFYRHIFDVCCELMETIQEIPQ
                     PVIAQVHAAATAAGCQLAATCDLVVASEKARFATPGVRIGLFCSTPMVALSRAVGQKK
                     SMEMLLTGEFISAEEALSEGLVNRVVSAEDLETETRSLAEKIAEASPLVVGIGKQAFY
                     RQLGLPTEQAYAYTKEVMSFNASFADAQEGMGAFLEKRKPEWKGR"
     gene            complement(6008..>6405)
                     /locus_tag="VNT12_01165"
     CDS             complement(6008..>6405)
                     /locus_tag="VNT12_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWI44748.1"
                     /translation="GWYHSGDVAVWHPDGYVEIRDRDKDIIISGGENISTVEVEQAVS
                     RHPAVMEAAVVAIPDEKWGERPKAFVVLKQGQEATEEEIIDFCKEHIARFKAPAAVEF
                     TELPKTSTGKVQKFVLRDKEWAGSEKQVN"
BASE COUNT         1316 a         1967 c         2065 g         1057 t
ORIGIN      
        1 gccctcgcca agaccgcgac cagcgccctc gaagcggaga agatgggctt cctcaccgaa
       61 aacgaccgca cagtaatgaa cgcagaccac ctcatctccg cagccaaacg cgaggccctg
      121 gaccttgccg acggctacac accgcccgag cacgccaaca acgtctacgc cgccggtcgc
      181 accgcgcgtg ccgcactgga gataggcata aagaccatgc agtggggcca ctacgccagc
      241 gagtacgacg gcgtcatcac aggccacgtc gcccgaatcc tcaccggtgg caacctctcg
      301 ctcccccaat gggtccccga agaatacttg ctaaagctgg agaagaaagc attcctggac
      361 ctcctgaaac aggagaaaac ccacgaacgc atcgaagcac tactgaaaac aggaaagccg
      421 gtacggaatt aggtatcagg tacaggcttc aggtatcagg taagactcgc gcaggagaaa
      481 cctgatgcct gtagcctgag acctgaaacc tgatcgaaag gagcgaagtt gaaagaagcg
      541 gtaattgttt caggagccag gaccgctgtg ggacgtgcgc cgagggggac tttgcgggcc
      601 agccgtcccg acgacatggc cgccgccgca atcggtgagg ccgtaaggcg tgcggagggg
      661 ctcgaaagag aagacgtgga agacgtcatc atcgggtgtg cgatgcctga ggggacgcag
      721 gggtacaacg tcgccaggct gtcttcactg ctcgccggga tgccggacac ggtgcctgcg
      781 cagacggtca accggttctg ttcttcggga ctccagacta tcgcgatggc ttccgagcgg
      841 atcataatcg gcaacgcgac gactatcgtg gcggggggga cggagcacat gtcttcgacc
      901 gtcgagatgc ccaacttctc gcccaatccg cggctcatgg agatcaaccc cgcagcctac
      961 atgggcatgg gcctgaccgc ggagcaggtc gcccgcgagt tcgacgttag ccgcgaagat
     1021 caggatgagt tcgccctgcg cagccacact ctagcgaaag aagccgtcga gggcggtgta
     1081 ttcgacgagg agatcgtgcc cctcgccgtc gagttcgact gggtcgatga gagcggcgaa
     1141 gcacagcaca tggagacgag cttcgggcgt gacgaaggcc cgcgcgacac ctcggccgag
     1201 aagcttgcca acctgaagcc tgccttccag caggatggca ccgttacggc gggcaactct
     1261 tcgcagagga gcgacggcgc ggcagcggtc gtggtgatgg acagagagga ggccgagaga
     1321 cgcgggctcg cgccactcgt ccgcttcgtc ggtttcgccg taggtggggt caggcccgag
     1381 gtcatgggca tagggccgac agtggcgata ccgaaggtcc tcgagcagac cgggctaacg
     1441 atggacgaca tcgacctcat cgagttcaac gaggcgttcg cggcccaggc tctcgccgtg
     1501 atccgtgagg tcgggatgga cctcgagaag accaacgtca acggcggagc catcgcccta
     1561 ggccacccta tgggcgcgac cggcacgaag cttactgtcc agctgatcca cgagatgaag
     1621 cgccgcggct cgaagtacgg tctcgtcacc atgtgcatcg gcggcggtat gggcgccgcg
     1681 ggtatcttcg agaacctgca gaactgagct ggacggcctg tcggcaaaga gtaggcacga
     1741 tgaacggacc cgtgtggaac catcggcgtc tgaaggcgaa cgggatcggc attcactacg
     1801 tcggccacgg gagcggtagc cgatggtgcg ctcgccacct ccgaggttga tggtagcccg
     1861 ggcgcctcgg cgctctgcga gatctaggcg agcaaaggcg ggaatgacct ggggtcgact
     1921 gtcgatctgt tagagcagca cgaatgtgag tatggccaga tagagcatag cggcgacggc
     1981 catgatgaga cagaatccca ggttgcgtct gggtcctatc tccagcgggt gcagcccagt
     2041 cagccgcgag agcatggtta cggcgctgga aaacggggtg agcaagaggg tggactgggc
     2101 gccgagcagg agtgccgccg cccacagcgc aggagccggt ccgccgtcta gagggaaggc
     2161 ggtgttgagc aacagcgcca tcggtatgac gtggatgccg gcgacgaaac ccacgcccat
     2221 gatgagtgcg agcgctgggg caaccaggac cgtcggaacg gcagagagca atgcgcctag
     2281 cggcgcgagg gcgccaagag ctttcagaga gagtacgagg atgccggcag ctccgaacag
     2341 agcgaactcg gagtgcgagg ccgtgagcga gccgcgcgcc tcttgcccaa gccgtaccag
     2401 gggcgacgcc ccgagggtga gcaccgccgc gagcccggcg atagagagta cgatcgtcgc
     2461 gatcgtcaca gcgatggccg ccgtaagcgg caacgcggga aagctcaggt taaccagggc
     2521 gacggcagtg accagcacga cggggtagag gagaacagcg gcagcccctc ggttcagggg
     2581 cgcctgaggg atctcttcaa cctcgccccc cgccctctgg gcgaagagta acgtcccgcc
     2641 gaggccgatg agcacaaaag ggagcgtaac cgcgaggagc atcgggtagg agaccccggt
     2701 cagcacgatg gtcgtcgctg tcaggacgtt caggttggtc cacaggggtg cgaaggagaa
     2761 ggtgcgccca gcccacttca aggcctccag gcgctccttg ggtgcggagc ggcccgagat
     2821 cacgtctatg agcacgaacg ctcccacatc cagaacagcc ccgaggatgt gaccgagtac
     2881 cccagccgtc gcctttatac tcacgccccg gcgggtcagc gccgccatga gggcccggat
     2941 ctggacctca tagcgggcag cctggatcgg atagcccgcc atgctgagga cgagcaacac
     3001 agccacgatc ccgaagtacg taggcatgcc ttgtagaaag tccccgaggt cgaaccgtcc
     3061 ggtgaagatc accacgccta aagccaccaa ggcggccagg gcgaacgccc tgtggaaggc
     3121 gctcgaccac ggtaccgaga tcacgaacaa cacagcccct actcccagaa agaggtcagt
     3181 cccgaaagag tccggcgcga acgtctgcca agcgtacccg gccaggaaga ctataccgag
     3241 cacgggacgc aacgcatcca gatgcgccct cacgctcccc gcccctgctg ttttccagtg
     3301 ttctctctta cctctaaccc gcttcggagc cgccagaata tcgcccgcac tcacgattct
     3361 agagcatggg tgaagccagc agggagcgcc gcgttaacct ccagactgca ggccgcgaaa
     3421 ggatcgggtg tgcgccatcg agccagagga cgatcgccgg actgtgcgca atataatctc
     3481 cgtcgacgaa gcagtagaga gggcgtggac atgcaaggat ctgccaggat gggcggctcg
     3541 tttggcaaga ctgtgctgct ggtcgtcctg ctcgttctca tgtgcgcggc gtgcggcggg
     3601 tccgcggaag agagaggaaa gagtgacaac gacatagagc cgattaccac agggcaagga
     3661 gagaaagagg aggacacctc gaatcagggt ggggcttccg tggagcaggt cgaggtgagc
     3721 accctcgcca cggacttgga cgtaccctgg tcgttcgcgt ttctacctgg tggcgacgcc
     3781 ctcgttaccg agcgcgactc ggggaggctt ctgcgggtga gtcgctcagg cgacgttcag
     3841 gagatccaga cgctgcctga gggtggctcg ggcgagggcg ggcttctcgg cgtcgccgtc
     3901 tcccctgact acgaggatga ccgctatatc tacgcctact acacgaccgg ggtagacaac
     3961 cgggtcgtaa ggttccgcaa gggcgagagc cttgagccca tactgacggg catcccctcc
     4021 aacacatatc acgatggagg taggatcaag ttcggtcccg acggcatgct ctacgtgagc
     4081 acgggcgacg caggagagtc cgggaactcc cagaaccgga actctctggg aggtaagatt
     4141 ctgaggatag agcccgatgg ttccattcca ccggacaacc cgttccccgg caaccccgtc
     4201 tattcgtacg gccaccgcaa cgtggagggg cttgcatggg actcggaggg acaactctac
     4261 gcgagcgaat tcggcgcgag cacctgggat gaggtcaaca gggtcgaagc cggggagaac
     4321 tacggctggc ccgcggtcga gggcaggggt ggagaggatc aaggctacgt cgaccccatc
     4381 accgtctggc cgacctcgga agcctccccg agcggggctg agatcatggt cgaaggggca
     4441 gtaccgcaat gggaaggcga cctcttcgtg gccgccctcc gcggagagcg gctctggcgc
     4501 ctggagttga acgacagcgg cgaggtcgtg gatagggaga agctcctcga cggagaagtc
     4561 ggccgcgtgc gggatgtcat acaggccccc gacgggtccc tgtgggtttc gacctccaac
     4621 cacgactcct acgctagccc ggtcagcgag caggatgacc gcatactacg cctcgcgcct
     4681 gccagagaat agaatgagcc cggtggaaga cacgctgaga agtacacagg atgcctaccc
     4741 ggcagacctc gcccgcttcg tccgcgagcg gtgggacgaa gatagagcca gtacgctgcc
     4801 cgaccaggaa ccaggcgtag gccggcgcct cagccggatg cgggctctac ggtcgcggac
     4861 atagagcctt tgcagcgcgg gatcagcgga tcgggaccga aggcctggat ctgatcccgc
     4921 ttcaactccg cctgctccag gttggtggtc gccacgacga cccgccctgt agcgtcgacc
     4981 tcccgcgcca tctcataacc cttctccaca ggatgcccga agactttctt gagcatgaag
     5041 atcacatact cgtagctgtg atcgttatcg tcgagcaaca ccacgttgta gggcggcagc
     5101 ccctcggtct tctgctcttc cttctctacc ggccgggcaa tcgtatctgt catggtgaac
     5161 tcctgatacc agtcgtgcgg aagccccggg gcttccgcac gcctttgtca gcgtcccttc
     5221 cactccggct tgcgcttctc caggaaggcg cccatgccct cctgggcgtc tgcgaacgag
     5281 gcgttgaagg acatgacctc tttggtgtag gcgtatgctt gctccgtggg caacccgagc
     5341 tgtcggtaga acgcctgctt gccgatcccc acgaccagag ggctggcctc cgctatcttc
     5401 tcggccagcg accgtgtctc ggtctcgagg tcctcagccg aaactaccct gttgacgagt
     5461 ccttcagaga gggcttcctc ggctgagatg aactcgcccg taaggagcat ctccatagat
     5521 tttttctgac caactgcgcg ggagagtgcg accatcgggg tagagcagaa cagacctatg
     5581 cgtacaccag gtgtagcgaa ccgtgctttc tcggacgcga ccacaagatc gcaggtggcg
     5641 gcgagctggc agcctgccgc ggtggcggcg gcgtggacct gtgcgatcac aggctgtggg
     5701 atctcctgga tggtctccat caactcgcag cagacgtcga agatgtggcg gtagaatcca
     5761 gggtctcgcc cgatcatctc cgaaaggtcg tggcccgcgc agaaggcggg gccctcgccg
     5821 cgcaggatga ccaccgaagc gtccttgcgt tctcctatct cctcgaagag agagatcaac
     5881 tcccgcatgt gctcgagtga gagtgcgttc ctcttcttcg gccgattcat ggtgacgtat
     5941 gcgacagcgc cgtcctgctc gaacagaatg tgctcgtagg ttttctcgac ttcgatcatg
     6001 gcgtctccta attcacctgc ttttcgctgc cagcccattc cttgtcgcgc aggacgaact
     6061 tctggacctt gcccgtcgag gtcttgggca gctcggtaaa ttcgacggcc gctggggcct
     6121 tgaagcgggc gatgtgctcc ttgcagaagt ctatgatctc ctcctcggtc gcctcctgcc
     6181 cctgcttgag cacgacgaac gccttgggac gctcgcccca cttctcgtct gggatggcga
     6241 cgaccgcggc ctccattacg gccgggtggc gcgacacggc ctgctccacc tcgacggtcg
     6301 agatgttctc gccgccggag atgatgatgt ccttgtctct gtccctgatc tccacgtacc
     6361 cgtcagggtg ccacaccgcc acgtcaccgg aatggtacca gccac
//
LOCUS       DATMJA010000049         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_481080, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000049 DATMJA010000000
VERSION     DATMJA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481080"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="moeB"
                     /locus_tag="VNT12_01170"
     CDS             complement(<1..888)
                     /gene="moeB"
                     /locus_tag="VNT12_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWI44749.1"
                     /translation="MQTRQPLVESPNGALELSNEEIARYSRHLIMPEVALEGQKQLKA
                     AKVLTVGTGGLGSPLALYLAAAGVGTIGIVDFDVVDESNLQRQIIHGTSDVGRPKVQS
                     AYEKIKDINPNVEVRVHGEALTSENALEIFEDYDVIVDGTDNFPTRYLVNDACVLLNK
                     PNVYGSIFRFEGQASVFYAEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGAIGTIQ
                     ATETVKLILGIGEPLIGRLLLYDALNMRFREMKLRKDPDCPVCGENPTVTELIDYQEF
                     CGISQASAAEEENGVPEITV"
     gene            complement(935..1216)
                     /locus_tag="VNT12_01175"
     CDS             complement(935..1216)
                     /locus_tag="VNT12_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWI44750.1"
                     /translation="MPKVKIPTPLRQYTDGNTEVEVGGATAGEALGNLASEHPDLKQH
                     LYTGDGKLRSFVNVYKGDEDIRYLEGQDTEVTDGDEISIIPSIAGGRCR"
     gene            complement(1229..1648)
                     /locus_tag="VNT12_01180"
     CDS             complement(1229..1648)
                     /locus_tag="VNT12_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWI44751.1"
                     /translation="MPLKIGAGDLGQIHDHAREAYPEECAGALVGIDSGGIKVVVDVW
                     RAENTHEEERSRRFLIEPLKIKRFEEQAEEQDMGLLGFYHSHPDHPAEPSEYDREHAW
                     PGYSYVIASLGAENVEDMRSWTLRDDRSGYEEEPIVG"
     gene            complement(1649..>2138)
                     /locus_tag="VNT12_01185"
     CDS             complement(1649..>2138)
                     /locus_tag="VNT12_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWI44752.1"
                     /translation="RYFYPDQYSNPANPRAHYESTAPEIWEQTNGEVTHFVAGLGTSG
                     TFVGTATRLKEYNPAIEAISFEPDSAFHGLEGMKHMESAIVPAIYDPTIADQNRAART
                     ENAYEMVKRVAREEGILIGLSAGAAVATALEVAREIESGTVVTILCDGADKYLSEKFW
                     EE"
BASE COUNT          346 a          648 c          669 g          475 t
ORIGIN      
        1 gaccgtgatc tccggcacgc cgttctcctc ttcggcggcg ctcgcctgag agatgccgca
       61 gaactcctgg taatcgatga gctccgtaac cgtagggttc tcgccgcaga cggggcagtc
      121 cggatccttg cggagcttca tctcgcggaa acgcatgttc agcgcgtcgt agaggaggag
      181 ccgtccgatg agcggctcgc cgatcccgag tatgagcttg acggtctcgg tggcctggat
      241 ggtaccgatg gcgccaggca ggatgccaag cactccgccc tcggcgcagc tagggacgag
      301 gccgggagga ggtggctcgg ggtagaggca gcggtagcag ggaccttcct cggcgtagaa
      361 cacgctcgcc tggccctcga accggaagat cgaaccgtag acgttgggct tgttcagaag
      421 gacgcaggcg tcgttcacca ggtaccgggt cgggaagttg tccgtgccgt cgacgatcac
      481 gtcgtagtcc tcgaagatct ccaacgcgtt ctcgctggtg agcgcctccc cgtggaccct
      541 gacctcgacg ttggggttta tgtccttgat cttctcgtag gcgctctgga ccttgggacg
      601 acccacgtcc gaggtgccat gaatgatctg gcgctgaagg ttcgactcgt cgacaacgtc
      661 gaagtccacg atcccgatag tcccgacccc ggccgcggcc agataaagtg cgagggggct
      721 cccgagtccg cccgtgccta ccgtgagtac cttcgcggcc ttgagttgtt tctgtccttc
      781 gagggcgacc tcgggcatga tcaggtgcct gctgtaacgt gcgatctctt cgttgcttag
      841 ctcgagtgcc ccgttgggcg actccaccaa tggctgtctt gtttgcatct tgagataaac
      901 ctcttgtttg gaaaaaaagc gaccttctga aaaactacct acatcgtcct ccggcgatgg
      961 aggggattat ggagatctca tcgccatcgg tgacctcggt gtcttggcct tccaggtaac
     1021 ggatgtcctc gtcgcccttg tagacgttga cgaacgagcg cagcttgccg tcacctgtat
     1081 acaggtgctg cttgaggtcg gggtgctctg aggcgaggtt ccctagcgct tcgccagccg
     1141 tggcgccgcc aacctcgacc tcggtgttac cgtcggtgta ctgtctgaga ggggttggga
     1201 tcttgacctt aggcatatct gagtctcctt atcctacgat tggctcttct tcgtaacccg
     1261 agcggtcgtc tctgagggtc catgagcgca tgtcttctac gttctcggcg cccagcgagg
     1321 ctatcacata ggagtagcca ggccaggcgt gctcgcggtc gtactcggaa ggttcggctg
     1381 ggtggtcagg gtgtgaatgg tagaagccga gcagccccat gtcctgctct tcggcctgct
     1441 cctcgaatct ctttatcttg agcggctcga tgagaaagcg gcggctgcgc tcctcctcgt
     1501 gcgtgttctc ggcccgccac acgtcgacga cgaccttgat gccgccgctg tcgataccga
     1561 caagcgcacc ggcacactcc tcagggtacg cttctctggc gtggtcgtgg atctgcccca
     1621 agtcccctgc gcctatcttg agcggcactt actcctccca gaacttctcg gagaggtact
     1681 tgtcggcccc gtcgcacagg atggtgacga ccgtacccga ctctatctcc ctcgcgacct
     1741 cgagcgccgt tgcgaccgcc gcgccggccg agaggccgat gaggattcct tcctcgcgcg
     1801 ccacccgctt gaccatctcg taggcgtttt cggttctcgc cgcccggttc tggtcggcga
     1861 tggtcgggtc gtagatggct ggaacgatgg cgctctccat gtgcttcatg ccttcgaggc
     1921 cgtggaaggc cgagtcgggt tcgaaagaga tagcctctat ggccgggttg tactccttga
     1981 gacgcgtcgc cgtaccgacg aaggtgccgc tcgtgccaag ccccgcaacg aagtgtgtga
     2041 cctcgccgtt agtctgctcc cagatctcag gcgccgtcga ttcgtagtgc gcgcgcgggt
     2101 tggcggggtt ggagtattgg tccggataga agtagcgt
//
LOCUS       DATMJA010000050         3445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_244449, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000050 DATMJA010000000
VERSION     DATMJA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244449"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VNT12_01190"
     CDS             complement(<1..231)
                     /locus_tag="VNT12_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI44753.1"
                     /translation="MEERRITERPPDEVEDPSQDVERTVAVELSAVTKRFGEFIAVDE
                     LTLDIYEGEFFSLLGPSGCGKTTTLRMIAGFEE"
     gene            complement(476..1912)
                     /locus_tag="VNT12_01195"
     CDS             complement(476..1912)
                     /locus_tag="VNT12_01195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI44754.1"
                     /translation="MTRAPSARKSVSPEDKDYRSYSFWLETSGEDLLPRPSLPGRAEA
                     DVAILGAGFTGLWTAYYLLAREPSLKVVVLEAEIAGFGASGRNGAWCSSGFPVSPGEL
                     QRRFGREAARELLLEMRDAVEEVGRVARAEEIDAQYFRGGQLRVARGPSQLPGIQEAY
                     ESLCGLGLEDGVRLLDADEMAGRVRITGARGALFNPHCATIHPARLARGLAGAVERLG
                     GEIFERTPVTDYEEGGENGSGPRLVTDSGEVRAHAIVLAGEAYLARLRKLRRQLIPIY
                     SLIVLTEPLTEERWAGIGWEGRECVASNRYTVDYLSRTEDGRILFGGRGAPYHYGSRL
                     RDEYDRHEPTHQMLRSTARDWFPALEGARFTHAWGGPLAVPRDWMPTMSYNPSSGVAT
                     ARGYTGQGVATANLSGRTLADLILKRQTAIIGLPTVDHKQRPWEPEPLRWLGARYVQR
                     GLMKVDDNAERTGEPPTGKTLAERLGRH"
     gene            complement(1924..3288)
                     /locus_tag="VNT12_01200"
     CDS             complement(1924..3288)
                     /locus_tag="VNT12_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWI44755.1"
                     /translation="MDVMRGLEESGSGAPLPGVENLMLHFTPYSEDWSKLPVIVSGEG
                     CYVTDDKGNSYIDGLAGLFTTQVGHGRSELAEVAAKQMKELGFFPNWGMHHPRSLELA
                     ARISEIAPGDLSSTFFVSSGSEAVETVIKLARQYHKANGEAERYKVVSRKIAYHGTTM
                     GALSVTGLPAFKAPFEPLLQGFYHVPNTQQDPEGAADAIEEAIKFGPPETVAAVILEP
                     VQNAGGCLVPPPDYWRRVREICDRHGVLLVSDAVICAFGRLGEWFGIERFDVVPDMTS
                     FAKGVTSGYLPMGGVVVNEKMSATLRDNAPMFLHGSTFGGHPVSSAVAIENIDIIERE
                     GLLKNVHDLEGHFEGELNRMAADHPIVKEVRGMGYFWAVEVKPERADGTPLVGDEYQK
                     YFKGVVSKKLLEGGLICRFDDKDEPVIQFSPALVADKEIISKIAGITDDALTELEREL
                     GYRG"
BASE COUNT          599 a         1176 c         1006 g          664 t
ORIGIN      
        1 ttcttcgaag ccggcgatca tgcgcagcgt cgttgtcttg ccgcacccgc tcgggcccag
       61 cagcgagaag aactcgccct cgtagatgtc gagcgtgagt tcgtcgaccg cgatgaactc
      121 cccaaaacgc ttggtaaccg ccgacaactc cacggcgacg gtacgttcca catcctggct
      181 ggggtcttcc acctcgtctg gcggacgctc tgttatacgt cgctcttcca ctctcagcct
      241 cctgtagcgt ccataacggc cttcaccgtc ttagataacc ctccccctcc agattctatt
      301 ccagccagcg gccatgtgtg aagtcgaagc actgcctggc tcgaacgggc ccaggggaga
      361 cgacccaccc ataccaggga ggcgtgcgtc accgtcagtc tccacgcctt gtgatcgtcc
      421 gatgacttct ctccttcaac ccttcttctc ccgccgcgcg agtccaacta gatcttcagt
      481 gtctgccgag ccgttctgcg agggtcttgc ctgttggcgg ctctccggtc cgctcggcgt
      541 tatcatctac cttcatcagg ccgcgctgca cgtacctggc gcccaaccag cgcaggggtt
      601 ctggctccca gggacgctgc ttgtggtcga cggtgggcaa accgattatg gcggtctgac
      661 gtttgaggat caggtcggcc aaagtccggc ccgagaggtt ggctgtcgcg accccctgcc
      721 ccgtgtagcc acgcgcggtg gctacgccac tggatgggtt gtaggacatg gtcggcatcc
      781 agtcgcgggg tacggccagc ggccctcccc acgcgtgggt gaaccgcgcg ccttcgagcg
      841 cagggaacca gtccctcgcc gtgctgcgca gcatctggtg cgttggctcg tgcctgtcgt
      901 actcgtcgcg taagcgcgat ccgtagtggt atggcgcccc cctgcccccg aacagtatgc
      961 gcccgtcttc ggtgcgcgag aggtagtcga ccgtgtagcg gttggacgcc acgcactcac
     1021 gcccctccca gccgatgcct gcccaccgct cctcggtcag aggctcggta aggacgatga
     1081 gggagtagat ggggattaac tggcggcgca gcttgcggag tcgcgctagg taggcctcac
     1141 cagcgagcac gatcgcgtgc gcgcgcacct cgccagagtc cgtcaccagc ctcgggcctg
     1201 agccgttctc gccaccctcc tcgtagtccg tcacgggggt ccgctcgaag atctcaccgc
     1261 cgagccgctc gacggccccg gcgagcccgc gcgcgagccg cgcagggtgg atggtcgcgc
     1321 aatgcggatt gaacaacgcg cctctggcgc cggtgatcct gacccgccct gccatctcat
     1381 ccgcgtcgag caacctgacg ccgtcctcca ggccaaggcc gcacagcgac tcgtacgctt
     1441 cctgtatacc tggtagctgg ctcgggccac gggcgacgcg gagctgtccg ccacggaagt
     1501 actgtgcgtc gatctcctcc gccctggcta ccctgcctac ttcctcgacc gcgtcccgca
     1561 tctctagcaa gagctcccgc gctgcctctc tcccgaaacg ccgctggagc tccccaggac
     1621 tgacgggaaa gcctgaagag caccaggccc cgttgcgccc ggaggccccg aagccggcga
     1681 tctccgcctc gagcaccacc acctttaacg aaggctccct agcgagcaga tagtaggctg
     1741 tccacaggcc cgtaaagccg gcgccgagga tggccacgtc ggcctcggcc cgaccgggta
     1801 gcgaaggcct agggaggaga tcctccccag acgtctccaa ccagaagctg taagagcggt
     1861 aatccttgtc ttcggggctg acgctcttcc gtgcactagg ggcgcgggtc aaactctacc
     1921 cctctaaccc ctgtagccga gttctcgttc gagctcagtg agggcatcgt cggttattcc
     1981 tgctatctta gagatgatct ccttgtctgc tacgagcgcc ggggagaatt ggatgacagg
     2041 ctcgtccttg tcgtcgaagc ggcagatgag accgccctcc aatagcttct tcgagactac
     2101 gcccttgaag tacttctggt attcgtcgcc caccagcggc gtaccatcgg cccgctcggg
     2161 cttgacctcg acggcccaga agtagcccat ccccctgact tccttgacga taggatggtc
     2221 ggcggccatc ctgttcagct cgccttcgaa gtgtccctcg aggtcgtgga cgttctttag
     2281 cagcccttcg cgctcgatga tatctatgtt ctcgatggcg accgcgctcg agacggggtg
     2341 ccccccgaag gtcgagccat gcaagaacat cggggcgttg tccctgagcg tcgcgctcat
     2401 cttctcgttc accacaaccc cacccatcgg caggtagccc gacgttacgc ccttggcgaa
     2461 gctcgtcatg tcagggacca cgtcgaagcg ctcgatgccg aaccactcgc ctagccgccc
     2521 gaaggcacag atcaccgcgt ccgagacaag cagcacacca tgcctatcgc agatctcacg
     2581 cacccgtctc caataatccg ggggcggcac caggcaccca ccggcgttct gcacgggctc
     2641 caggatcacg gcagccaccg tctctggcgg cccgaacttt atggcctcct cgatagcgtc
     2701 ggcagccccc tcagggtcct gctgggtgtt cggtacgtga tagaagccct ggagcaacgg
     2761 ctcgaagggg gccttgaacg caggcaaccc cgtcacggag agcgcgccca tcgtggtgcc
     2821 gtggtaggcg atcttgcgcg atacgacctt gtagcgctcg gcctcgccgt tcgccttgtg
     2881 gtactggcgg gccagcttga tgaccgtctc gacggcctcc gagccggagg agacgaagaa
     2941 ggtggaagag aggtccccag gcgctatctc ggagatcctg gccgcaagct ccagcgacct
     3001 gggatggtgc atgccccagt tggggaagaa cccgagctcc ttcatctgct ttgcggcgac
     3061 ctcggccagc tcgctcctcc catgcccgac ctgcgtcgtg aagagcccgg caagcccgtc
     3121 tatgtaagag ttgcccttgt cgtcggtgac gtagcacccc tcaccagaga cgattacagg
     3181 cagcttggac cagtcctctg agtaaggtgt gaagtggagc atcaggttct ccacaccagg
     3241 aagtggcgcg ccgctcccgc tctcttcgag acctctcatc acatccattt aatacctcct
     3301 gcaggcatgg atacccggct cctagctttg cacgaatact acacagttac tgtaatacgg
     3361 tcaagatcca acgagcgacg tgagatcggg gtctgaccac tgtaacgcta ttgtaactgg
     3421 tgtgtcgagg aatggagcct cttca
//
LOCUS       DATMJA010000051         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_659042, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000051 DATMJA010000000
VERSION     DATMJA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659042"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VNT12_01205"
     CDS             complement(<1..658)
                     /locus_tag="VNT12_01205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI44756.1"
                     /translation="MRRVEPLINLALLLPRRPREFRDRVAGYADLSLERLWRSSPAYE
                     TAMWQGVLADIEVHLGRVTEILDEPALKEVEDDTRRLLEDIRARDPFSPRWAADSRFA
                     RLGYVLCRLISPSVVLETGVAYGVSSVFILKALEVNGRGTLHSVDLPPLRSEYKRFWG
                     IAVPEALKDRWRLHRGSSARVLPPLLREIRTIDLFVHDSLHTRGNMRREFEAVWPHLR
                     N"
     gene            751..1368
                     /locus_tag="VNT12_01210"
     CDS             751..1368
                     /locus_tag="VNT12_01210"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HWI44757.1"
                     /translation="MKWLKVYGLPKLPLFLARWAMKLGLPGPTWYKWKAASAGTGVIL
                     DEMIRAADDLGYDGQKVGKLAMTRIGEKQGSDLREQLGVKTMKDAVDVVMLANRFFDI
                     EITLTEQGDGTYVINADRCPWFGGMGQEGVPGWDVKPCAAFSTYEKAMVQAINPNVKL
                     SYTEKRTTGGHTCRGVYQYRDEELGDGPADGVQPEMVSIGKKPKA"
     gene            complement(1400..2347)
                     /locus_tag="VNT12_01215"
     CDS             complement(1400..2347)
                     /locus_tag="VNT12_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI44758.1"
                     /translation="MKVGGAARPVKVLYVAGLGRSGSTILANTLGQVEGFFSGGELNF
                     IWKHALIENRLCGCGKPSQECEFWGPVFDTEFGGQSEELAREMMRLQYSGARTRHIPL
                     MLTEGGRQKIRERLGKFLDNTGRLYRAIQSVSDSCVVVDTSKEPAYGYALGMVPGVDL
                     RVLHLIRDPRAAAYSWAKKKRQPDSTEREFMHRKTPMQSAVLWDAWNAAIEALWRQMP
                     ARYLRLRYEDFVTDPRRSFEEILELAGEEDAQLPLVGERDVKLGISHTVSGNPNRFDT
                     GTVELRQDRAWQKQMRPRDRALVTALTLPLLTRYHYSVR"
     gene            complement(2344..>3333)
                     /locus_tag="VNT12_01220"
     CDS             complement(2344..>3333)
                     /locus_tag="VNT12_01220"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWI44759.1"
                     /translation="LAMFLAGLVGLYYLRKLFPDLFDRRAQTTFETKALFGVSIPMSI
                     TQGAQYLNNFSAILILGVFAAGAPFGIFNAAARTATFLTAVRFAFSGIFSPIISGLYA
                     RQNMGEMGRLYKDVSRWIFTGAFALFLVIVVFGPEVMGIFGEAFGAGVTALVIVAVAQ
                     LYSSSVGPAPRMLAMTENQNFAMIATSVAAITGVVVNFILIPRFEVLGAALGMATAII
                     VENTGTMSAVKWRLGYWPFNIMWLKPLTAGAISATATYLLKITLPLEAMFGFLPVPVS
                     VPTIMLLGGFLGVLFFGLLWLFGFSETDKEFLGTFWNVAQRALPKRFKKGNQE"
BASE COUNT          668 a         1014 c         1071 g          580 t
ORIGIN      
        1 cgttccgaag atggggccat acggcctcga actcgcggcg catgttcccg cgggtatgca
       61 ggctgtcgtg tacgaaaagg tctattgtgc ggatctctcg gaggagcggg ggcagaaccc
      121 tggcgctgga cccccgatgt agtctccagc ggtctttgag cgcttctggt acggctatgc
      181 cccagaacct cttgtattcg ctcctcaagg gaggcaggtc tacgctgtgg agtgtgccgc
      241 ggccgttcac ctcgagggcc ttcaggataa agaccgagct cacgccgtag gccacgccgg
      301 tctccagcac tacggaaggt gagatcagac ggcacaaaac gtagccaagg cgggcgaacc
      361 tggagtccgc ggcccaacgc gggctgaacg gatccctagc gcggatgtcc tccagaagcc
      421 ggcgtgtgtc atcttcgacc tccttgaggg ccggctcgtc gaggatctcg gttacgcgac
      481 ccaggtgcac ctcgatatcc gcgagcacac cctgccacat tgcggtctca taggccggtg
      541 agcttctcca gagccgttcc aggctgaggt cagcataacc tgccaccctg tcgcgaaact
      601 cccggggacg ccgcgggaga aggagagcaa ggtttataag cggttcgacc ctacgcatgg
      661 ctcacggacg ctccttcggc actccacgct cggaggtcag agtataatgg gacttgcgcc
      721 agcgtaagcg aacgaagggt ttgccccatt atgaagtggc tcaaggtcta tggtcttcca
      781 aagctcccgc tgtttctggc gaggtgggcg atgaaactcg ggttgcctgg gccgacgtgg
      841 tacaagtgga aggccgcctc ggccgggacc ggcgtcatcc tggacgagat gatccgggcc
      901 gccgacgacc tgggctacga cggtcagaag gtgggcaagc tcgccatgac ccgtatcggc
      961 gagaagcagg gtagcgatct gcgcgagcag ctcggtgtga agacgatgaa ggacgccgtc
     1021 gacgtcgtaa tgctagccaa tcgctttttc gacatcgaga tcacactgac cgaacagggc
     1081 gacggaacct acgtcataaa cgcggaccgc tgcccttggt tcggcggcat gggacaagaa
     1141 ggcgtccccg gttgggatgt gaagccctgc gccgctttct ccacctacga gaaggcgatg
     1201 gtccaggcca taaaccccaa cgtaaagctc tcatacaccg agaagcggac tacaggcggc
     1261 cacacctgcc gcggcgtata ccagtaccgc gacgaggagc tcggcgacgg acccgcggac
     1321 ggcgtgcaac ccgagatggt cagcatcggc aagaagccga aggcgtaaag agcttcgagg
     1381 cgccggtggg ttgcgccggc taccttaccg agtagtggta gcgtgtcagc agcggcaatg
     1441 tcagcgctgt aacgagagcc ctgtcacggg gtctcatctg tttctgccac gcacggtcct
     1501 ggcgtagctc gacggtgccc gtgtcgaagc ggttggggtt gccggagacg gtgtggctta
     1561 taccgagctt gacgtcgcgc tcccccacca gcggcaactg cgcgtcttcc tcacccgcga
     1621 gctccaggat ctcctcgaag ctacgacgag ggtcggtgac aaaatcctcg tagcgcaagc
     1681 gaaggtagcg ggcgggcatc tggcgccaga gggcctcgat ggcggcgttc caggcatccc
     1741 agaggacggc gctctgcatg ggggtcttcc ggtgcatgaa ctcgcgctcg gtgctgtcgg
     1801 gctggcgctt cttcttcgcc caagagtagg cagcggccct ggggtctcgg atcaggtgca
     1861 aaacccgaag gtccacgccg ggcaccatgc cgagtgcgta accgtaggct ggctccttgg
     1921 aggtgtcgac gaccacgcag ctgtccgaga ccgactggat cgcccggtac aacctgcccg
     1981 tgttgtccag gaacttgcct agacgttccc ttatcttctg ccttccaccc tctgtaagca
     2041 tcagggggat atggcgggta cgggcgccgg agtattgcag ccgcatcatc tcgcgggcca
     2101 gctcttcgct ctggccgccg aactctgtgt cgaagaccgg tccccagaac tcgcattcct
     2161 gggacggctt gccacaaccg cagagcctgt tttcgatgag ggcgtgcttc cagatgaagt
     2221 tcaactcgcc cccggagaaa aagccctcca cctggcccag ggtgttggcc aggatcgtgc
     2281 tgccgctccg ccccagtccc gcgacgtaga gcaccttgac cggccttgcc gcgccgccga
     2341 ccttcactct tgattcccct ttttgaaccg ctttggcagg gctcgctgcg ccacgttcca
     2401 gaaagtgccg aggaactcct tgtccgtctc ggagaatccg aacaaccata ggagtccgaa
     2461 gaacagcacg ccgaggaagc cgccgagcag cattatcgtg ggaacggata ccggcaccgg
     2521 caggaacccg aacatcgcct ccagcggcaa ggtgatcttc aacaggtacg tcgcggtggc
     2581 agagatggcg cccgctgtca ggggcttcag ccacatgatg ttgaaaggcc agtatcccag
     2641 ccgccacttc acggcggaca tcgttcccgt gttctccacg ataatcgcgg tagccatacc
     2701 gagggcggct cccagaacct cgaagcgcgg gatgaggatg aagttcacga caacgcccgt
     2761 gatggcggcg acggaggttg cgatcatggc gaagttctgg ttctcggtca tggcgagcat
     2821 tcgcggcgca ggtccgacgg aagagctgta gagctgagcg acggcgacga tgacgagggc
     2881 cgtaacgcca gccccgaacg cctccccgaa aatccccatc acctcgggac cgaagaccac
     2941 gatcacgagg aagagcgcga acgcgcccgt gaagatccag cgcgacacgt ctttgtagag
     3001 cctgcccatc tcccccatgt tctgcctcgc gtaaaggccg gagatgatgg gggagaagat
     3061 gccactaaag gcgaagcgca ccgcagtcag gaaagtggcc gttcgcgcgg cggcgttgaa
     3121 gatgccgaac ggagccccgg cggcgaagac gcccaggatc aggatggcgg agaagttgtt
     3181 gaggtactgg gcgccctgcg taatgctcat cgggatagag acgccgaaga gagccttcgt
     3241 ctcgaaggtg gtctgcgccc ggcggtcgaa gagatcggga aagagcttcc tgaggtagta
     3301 gagtccaacg agcccggcga ggaacatcgc gag
//
LOCUS       DATMJA010000052         4487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_126103, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000052 DATMJA010000000
VERSION     DATMJA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126103"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VNT12_01225"
     CDS             complement(<1..535)
                     /locus_tag="VNT12_01225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWI44760.1"
                     /translation="MSMWKNLASVMEGEIVRLEPLARRHEERLFEAARDERVWRWMHY
                     DAGGSRERFHAWFEDALVASSAGKEAAFATVDAATGEPVGSTRYLALRPDHRGLEIGW
                     TWLAPARWQTGANVEAKLLMLEHAFERLGCLRVEFKTDSRNERSRAALAALPAKFEGI
                     FRKHMLVRGGERRDSAYY"
     gene            766..1557
                     /locus_tag="VNT12_01230"
     CDS             766..1557
                     /locus_tag="VNT12_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI44761.1"
                     /translation="MDHRTSGVAMSYRDLGEGHPLVLLHAFPLNGRMFESQMRAFSGS
                     HRVIAPDYPGFGRSPRTPAQPDIHYYAEGVRSLLDRLHLERVILGGVSMGGYVAFECM
                     RLFPEQVTALVLANTRAEPDSEEMRETRIETALSVAEEGVGVLVELQMERLLTPYTLQ
                     NDEELVENVRAMIFENSPDGVVAALGAMRERPDSTPLLGEIGVPTLVVGGEEDGIISP
                     EVVGAMAAKIPDARHVIIPRTGHLSNLESPEMFNATLADFLQSIR"
     gene            1658..2524
                     /locus_tag="VNT12_01235"
     CDS             1658..2524
                     /locus_tag="VNT12_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWI44762.1"
                     /translation="MAILAHISDLHFGRPAVAERLDALKEYITKIRPNAVAVSGDLTQ
                     RCTNREFSNARAYLDALGEIAPYTVVPGNHDIRWLGAVARNLGLAGFFREQAHKFKYS
                     RYTRHISEDLSPSLEVPEVVIAGVNTAHGITRGSITRRLRDLGVIGQVKHADVIRVRE
                     IFEHAAPDSARIVMMHHNPIRGELSCRHGLANTEQALHAFADLGTELILCGHDHQDAI
                     HTIERGAHGLIISTAGTISNRIRPGRASSFNVVEIKDHEIHITAHAWRQGRGFIPSED
                     RTFPRRTASRQA"
     gene            2586..2798
                     /locus_tag="VNT12_01240"
     CDS             2586..2798
                     /locus_tag="VNT12_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44763.1"
                     /translation="MWSRLTRGGDGLPREKVDAIVRLIDRYLAEEASLRNLQSDKQTI
                     HPRDLPPDKREALIEEVFEILKDARR"
     gene            complement(2842..3678)
                     /locus_tag="VNT12_01245"
     CDS             complement(2842..3678)
                     /locus_tag="VNT12_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWI44764.1"
                     /translation="MRYGFLIPTGDPRTVAELAREVEEAGWDGAFYWDGICVGGVDTY
                     DPWVVMAAMAMRTERVRMGAVLTPPARRRPWKLARETVALDHLSDGRLVLPVGLGALD
                     DGGFSKVGEPTDRKVRAQLLDESLEILTGLWSGEPFSYAGEHYRLDEMTFLPRPVQKP
                     RIPVWVVGAWPSRKSMGRALRYDGVLAATVGGNAESPSVTPETISEIKDYVGENREED
                     GTFDIVWEGQTPGEDPRQAASIVRPFAEAGATWWIESPWTPPNEPDDLRRRIKQGPPS
                     GD"
     gene            3854..4309
                     /locus_tag="VNT12_01250"
     CDS             3854..4309
                     /locus_tag="VNT12_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HWI44765.1"
                     /translation="MATWNSSLNRTAFSATLHCLTGCAIGEVLGVVVGTALGWSTVAT
                     IALAIVLAFFFGYGLTMLPPLRSGMALAAALPLAFASDTLSITVMEIVDNLIIVVIPG
                     AMVAGLGSPLFWGSLALALAVAFVAAFPVNRYLTSRGKGHAVVHEHHEH"
BASE COUNT          870 a         1496 c         1314 g          807 t
ORIGIN      
        1 tgtagtaggc agagtcgcga cgttcaccgc cacgcacgag catgtgcttg cggaagatgc
       61 cctcgaactt tgccggtaga gccgcaaggg cggcacgcga acgctcgttg cgggagtcgg
      121 tcttgaactc gacgcgcaag caccctaagc gctcgaaggc atgttccagc atcaggagct
      181 tggcctcgac gttggcgcct gtctgccagc gtgcgggcgc gagccaggtc caccctatct
      241 ccagcccccg gtgatcggga cgaagggcga gataacgggt gctgccaaca ggctcccctg
      301 tggctgcatc cacggtggcg aaagcggcct ccttccctgc cgaagaagca accagggcat
      361 cctcgaacca ggcgtgaaac ctctcgcggg acccaccggc atcgtagtgc atccagcgcc
      421 agaccctctc atcccgcgcg gcctcgaaga gccgctcctc gtggcggcgc gccagcggct
      481 ccagacgcac gatctccccc tccattacgc tcgccaggtt cttccacatg ctcattaccc
      541 ctcactcagg acccgtcaga agatatggta gcggaagggc cataggtttc aggttacagg
      601 catcaggcat cagggaaaga gtggagtcgg aaatcaccgg cccgaaacgc taccatcggt
      661 agcgaccatg gcagcgcaag gggaacgtta ctgcgagacc aaagctgaag tgctgacgag
      721 ccgcagtagg cggcgtgctg accagctaat caaaaggaga gcagagtgga tcacaggaca
      781 tccggcgtag cgatgtcgta cagggacctc ggggagggtc atcccctcgt tctgctccac
      841 gccttcccgc tcaacggcag gatgttcgag tcccagatga gggcattctc gggaagccac
      901 agggtcatcg cgccggatta ccctggattc gggcgttctc cccgtactcc ggcccagccc
      961 gacatccatt actatgcaga aggagtgcgc agcctcctcg acaggctgca tctggaacgc
     1021 gtgatcctcg gcggcgtctc catgggcggc tacgtcgcct tcgagtgcat gaggctcttc
     1081 cccgagcagg tgaccgccct cgtcctcgcc aacacccgcg ccgaacccga ctccgaggag
     1141 atgagggaga cccgcatcga aacggccctc agcgtcgccg aggagggagt tggggtcctc
     1201 gtggagctcc agatggaacg cctcctcacg ccctacaccc tgcagaatga cgaggagctt
     1261 gtggagaacg tgcgggccat gatcttcgag aactcacccg acggcgtcgt cgccgccctc
     1321 ggcgccatgc gggagcgccc ggactctacc ccgctcctcg gggagatcgg cgtcccaacc
     1381 ctcgtcgtcg gcggggagga ggatggcatt atctcccccg aggtcgtggg cgccatggcc
     1441 gcgaagatac cagacgcccg ccacgtgatc attccccgga ccggccacct gtcgaacctc
     1501 gaatcccccg agatgttcaa cgccaccctc gcggatttcc tgcagagcat ccgataggca
     1561 gggatcctac cccgaccagt tcccctactc ggttatcttc ggagggtaga cagggcgggg
     1621 tttggagcct ggggacgttc gggtagaatc gctgttcgtg gccatccttg cgcatatttc
     1681 ggatctgcac ttcgggcgtc ctgctgtcgc cgaacgcctc gacgccctga aggagtatat
     1741 tacgaagatc cggccaaacg ctgtggccgt ctccggggac ctgacgcaac gctgcacgaa
     1801 tagggagttt tcgaacgccc gcgcctacct ggatgccctc ggggagatag caccttacac
     1861 cgttgtcccc ggcaaccacg acatccgctg gctcggggcc gtggcccgca acctcggcct
     1921 cgccggtttc ttcagggagc aggcccacaa gttcaagtac tccaggtaca caaggcacat
     1981 ctcggaagac ctgagtcctt ctctggaggt acccgaggtg gttattgcgg gtgtaaacac
     2041 ggcccacggc atcacgcggg gttcgatcac ccgccgcttg cgggacctcg gcgttatagg
     2101 ccaggtcaaa cacgctgacg tgatacgtgt gagggagatc ttcgagcacg ccgcccccga
     2161 ctcagcccgc atcgtgatga tgcatcacaa ccccataaga ggcgagctat catgccgcca
     2221 cggcctcgcc aatacggagc aagccctcca cgccttcgcc gacctcggta ccgagttgat
     2281 cctctgcgga cacgaccatc aggacgccat ccacactatc gagaggggcg cacacggtct
     2341 gatcatctct accgcgggga ccatctcgaa tcgtatccgt cccggacgcg catcgagctt
     2401 caacgtcgtc gagataaaag accacgaaat tcacataaca gcccacgcct ggcgccaggg
     2461 aaggggcttc ataccctcgg aagaccgcac ttttccacgc cgcacggcat ccaggcaggc
     2521 ttgaaagtgc cccgacgata taatctgccg cgacaccgcc caccttcacg gaagggacgt
     2581 tcgacatgtg gagtagactg acccgcggcg gcgacggact gccccgggag aaagttgacg
     2641 ccatagtccg ccttatagac cggtatctgg ccgaagaggc gagcctgcgc aacctccagt
     2701 cagataaaca aactattcat ccccgcgacc tcccccccga caaacgcgag gccctcatcg
     2761 aagaggtctt cgagatcctc aaagatgccc gtaggtaggc gaagttcgcg gtaggccgtc
     2821 ggcggtcagc tttttgggtc gttagtcgcc gcttggagga ccctgtttta tgcggcggcg
     2881 gaggtcatct ggctcgttgg gaggcgtcca gggggattcg atccaccagg tggcccctgc
     2941 ctccgcgaag ggacgcacga tggaggccgc ctgcctcggg tcttcaccgg gcgtctggcc
     3001 ctcccatacg atgtcgaagg tgccgtcctc ttctctgttc tccccgacgt agtccttgat
     3061 ctcggagatg gtctcgggcg taacgctcgg cgattcggca ttcccgccga ccgtggccgc
     3121 aagcaccccg tcgtagcgta gcgcccgtcc catcgacttc ctgctcggcc acgctccgac
     3181 cacccataca gggatgcgtg gcttctgcac aggacgtggc aagaaggtca tctcatcgag
     3241 ccggtagtgc tcgcctgcat aggagaaagg ctccccgctc cacaacccgg tgagtatctc
     3301 cagactctcg tcgagaagct gtgcccgaac cttcctgtca gtcggttccc ccaccttcga
     3361 gaaaccaccg tcgtccaggg ctccaagtcc taccggcaag acgaggcgcc cgtcggacaa
     3421 atggtcgagg gcaaccgttt cacgggccag cttccacggt ctccgccggg ccggcggcgt
     3481 caacacggcc cccatcctca ccctctctgt gcgcatcgcc atcgcagcca tcacgaccca
     3541 cgggtcgtac gtgtccacgc caccgacaca gatgccgtcc cagtaaaaag cgccgtccca
     3601 acccgcctct tccacctcgc gggcgagctc cgcgacggtg cggggatcac ccgtagggat
     3661 caggaacccg taacgcaacc gtacctcccc gatgcctgga tctcacacca caaacgagta
     3721 tacagacccc aagacgcact ccaaccatcg aacgcagcgc ccggcagaga taagatgcct
     3781 ctcccgtctc tgtttacagg taccccctag gggtatataa tgatatcgat acaggcgcac
     3841 aggaaggatg tttatggcca cctggaacag ctcactcaac cggacggctt tctcggcgac
     3901 gctgcactgt ctgacgggat gcgccatcgg tgaggtgctt ggggtagtgg tcgggaccgc
     3961 tctggggtgg agcactgtag ccaccatcgc gctcgcgata gtgttggctt tcttcttcgg
     4021 ttacgggctg acgatgttgc cgccgttgcg gtctggcatg gcgctcgctg cggcattacc
     4081 gctcgccttc gcctccgaca cgctctccat cacggtcatg gagatcgtgg acaacctcat
     4141 catcgtcgtt atccctggcg cgatggtggc ggggttgggc agccccctat tctggggaag
     4201 cctcgccttg gctctcgcgg tcgcgttcgt ggcggcgttc ccggttaaca ggtacctgac
     4261 ctcacgcggc aaaggccacg ccgtcgtcca cgagcaccac gaacattagc ggtgtggggc
     4321 agcgagctgc cccacaccgc gctggtcagc atgtcagcac gcttcggcct gcagcctccg
     4381 cttgtcagca cttcagccag ttaggaaaca atgtgctgaa atgctgacta gctgaaatgc
     4441 gcggattgca aagaagttct ttgcaatccg ctctagcgac ggcggcg
//
LOCUS       DATMJA010000053         7192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_244527, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000053 DATMJA010000000
VERSION     DATMJA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244527"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VNT12_01255"
     CDS             complement(<1..374)
                     /locus_tag="VNT12_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI44766.1"
                     /translation="MERLGGEHEGPVSSIRQVAIASSIGTALEWYDYFLYGTAAALIF
                     NQLFFPEAEPLVGTLLAFATYGVGFVTRPIGGLVFGHFGDKIGRKAMLVITLIIMGVA
                     TSIIGLMPTYESIGIWAAILLVV"
     gene            complement(689..1480)
                     /locus_tag="VNT12_01260"
     CDS             complement(689..1480)
                     /locus_tag="VNT12_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWI44767.1"
                     /translation="MAAFSRFERPGVERDRAFFPRVPQRGVALYLQVADYLRGRIETG
                     KLSPGDQLPPETELAQSFHVSRHTIREALRYLKARGYIESRQGKGSVVRDRRRSLRVN
                     PVIGSINDLLQFASETVLKPVSVASEPASGEVAELLNLEPGDDVLRISALRCDENDVV
                     FGYTDVYIPTIFAEDLNPETIHNVPIYAQIERNTGIEVVGVEQRITAVGASEALVKHL
                     GLKRGGPVLRITRLYYEESGRPVEFAESFHHSSAYEYLIHLRKEI"
     gene            complement(1480..2679)
                     /locus_tag="VNT12_01265"
     CDS             complement(1480..2679)
                     /locus_tag="VNT12_01265"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI44768.1"
                     /translation="MACTPRLPLEGVRIIAVEQYGALPFGTMHLADLGAEVIKIEDPA
                     RGDIGRFVGPYAEDGDSLFYQSLNRNKRSVSLDLRSEFGRRVLHDLVRVSDAVANNLR
                     GDLPANLGLTYEALGKVNPRIVTASLTGFGLTGPRAAQPGYDYLAQALAGFMSVTGEP
                     DDPPIRCGLSIVDFMSGIAAALGIVSAVLSARESGRGMDVDVSLLDVAVSNLNYLAAW
                     YLNEGYLPSRAPNGAHPSLVPSQRFRTRNGYMYIMCNKEKFWANLCDVLRRPEWKQEP
                     RYRDFSARYENREWLLPELQEALLERTTEEWLEAFGDSVPCARVNTIDEALAEPQVLA
                     RDMIIEVPHDRRGTVKEISCPIKVDGAPVPRNAAPTLGGDTECVLRDVLGYLESEISK
                     IAESEAR"
     gene            complement(2682..3848)
                     /locus_tag="VNT12_01270"
     CDS             complement(2682..3848)
                     /locus_tag="VNT12_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI44769.1"
                     /translation="MDEKAQHALLERVHAFVEEEIKPVVREFEVEDKYPQGIVERMKE
                     LGLFGAIIPEEYGGLGLDVSAYARIIEDISRGWMSIAGIINSHLILAYDIYLSGTPEQ
                     RDRYLPDLCSGERRGGLSITESHAGSDVQAIRTEARRDGDHYVINGHKKWISNGIFGN
                     TFLVAVKTDPLAEPAYKGISAFIVEKGGSGFEAFRRPEKLGYRGIDSAEFVFENFRVP
                     ADNLLGGEEGKGFRHIMKGLELGRINVAARGLGVARAAFEDAIAYAQQREAFGKPISQ
                     HQAIQLKLADMAVRIEAARLLVRNAAEKFDRGERCDLEAGMAKLFASEAGVDNSLEAM
                     RIHGGNGYHKDYDVERYFRDAPLMAIGEGTNEIQRLVIARGLLKRHALNEGGQG"
     gene            complement(3984..5417)
                     /locus_tag="VNT12_01275"
     CDS             complement(3984..5417)
                     /locus_tag="VNT12_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWI44770.1"
                     /translation="MSDTVKRAVATSRRGSALSGQIAEVLRDTYHEDVPTEVVETAKA
                     LALDALGIGFASRSESFSERAWSGISRVAGEGGATVIGSLRTVDTGSAALMNGLLIHG
                     LDFDDTHLASVIHTSASALPAALAAGELEDRSGSEVLKAFVLGSEVTARIGMVAAGEF
                     HKWGFHPTGVVGAFGAAVAAGLLFGLDKEELATAQGIVGSMASGIFEFLEDGTWTKRL
                     HPGWAAHAGLTAASLAGAGFTGPGAVYEGRFGLYATHLGTRDLPLEMATEDLGERWEI
                     LKVAVKPYPACHFNHAFIDAALELRQEERFAVEDVERIICKIAAGEVETVCEPIKTKR
                     RPQTTYDAQFSLPYAVASAIVRGRRLGIKDFDEEGIGDERVLGVAEKVGYEVDPDSGF
                     PRHFSGEVVIHLRDGRRVSRREQINRGAPERPLGRAEIREKFRLNTSIGLSEEQAELV
                     ARKVENLDGLDSISDLTSALRVDPKHG"
     gene            5673..6674
                     /locus_tag="VNT12_01280"
     CDS             5673..6674
                     /locus_tag="VNT12_01280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWI44771.1"
                     /translation="MSQAEMRYGLFVSAQYPENADLGRILHEHLEQVRFAADNGFSSV
                     FAGQHFLSTPFQMLQPVPLLARLAAESGDLRVGCGILLAALLNPVELAEQAATLDVIC
                     GGRFVLGVGLGYRAEENLAFGVTSRRARVLESKLDVVRRLLEGERVTAEGPGYQLEDA
                     AIGVRPIAEPRPPIWMAANSDAAVKRAARVADTWFANPHVTVEELQRQLDFFRQERGS
                     APTELPAMREVCVRRTDDEAIDVARPFLDNKYKSYVSWGQSEALPPTDTLRRNWDELR
                     MNRFIIGSPETAIDQVRHHRNDLGIDHLVFRLQWPGLPHMEAMETLRWLVGEVIPAV"
     gene            complement(6749..>7192)
                     /locus_tag="VNT12_01285"
     CDS             complement(6749..>7192)
                     /locus_tag="VNT12_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI44772.1"
                     /translation="RVVLGAVADSAGPLRGYRVSFFLIGASFALWWFGDGFWFLVAFA
                     AVLGVGYGGFVALSPVVLAAFFGVERLGGLLGVLLTANGLGSALGPPAVGFVVDATGS
                     YDAPIAALMLLGLAAYAALLPLGRHVTARNSNHYDHPHLASRRRE"
BASE COUNT         1340 a         2308 c         2164 g         1380 t
ORIGIN      
        1 acgactagca gaatggccgc ccagatgccg atggattcgt aggtcggcat aagacctatg
       61 atgctggtcg cgacccccat gattatcaac gtgatgacca gcatcgcctt gcgcccgatc
      121 ttgtcaccga agtggccgaa gacgaggccc ccgatcggcc gggtgacgaa gccgaccccg
      181 tatgtggcga aggcaaggag tgtccccacc agaggctcgg cctccgggaa gaacagctgg
      241 ttgaagatca aagcggctgc cgtcccgtac aggaagtagt cgtaccactc cagggcggtg
      301 ccaatggagc tggctatcgc tacctgcctt atagaggaca ccggtccctc gtgctctccg
      361 ccgagcctct ccatgtctct cctttcccta gaattaggat ccatttcccc gaagttgggg
      421 attattctat atgacacaaa gtgcttccgc actgctcgcc gagttcgagc acgcccatca
      481 tccttcctcg gtgtaccatc cgtccagagc acggatgagg cccgcttctc aaggctacgg
      541 ctttgtccaa gcggtccgga cggtcaccta gcacgaagaa gagagacctg gcgatcatgg
      601 cctactcccc ggtccgggac ccagctgtct caaggttcga ccgtgttccg tcacgagatg
      661 atcgagcccg ttcttcatag aacgcccttc aaatctcctt gcgaagatgg atcaggtact
      721 cataggcgga ggagtggtga aaagactctg cgaactcgac gggcctgccg ctctcctcat
      781 agtagaggcg ggtgatccgc aggacgggcc ccccgcgctt caggcccaaa tgtttcacga
      841 gtgcttcgct ggcgccgacg gcggttatgc gctgttccac gcccaccacc tcgatcccgg
      901 tgttgcgctc tatctgagcg tagatcggca cgttgtgaat tgtttcaggg ttcagatcct
      961 ccgcaaaaat ggtgggtatg tagacgtctg tgtagccgaa gaccacgtcg ttctcgtcgc
     1021 aacgtagcgc gcttatgcgc agcacgtcgt ctcccggttc gagattcagg agctcggcga
     1081 cctcgcccga agcgggctcg ctggctacgg agaccggctt cagcacggtc tcgctggcga
     1141 actgcagcag gtcgtttatc gaccctatga cgggattcac cctcaggctc cgtctacggt
     1201 ctcggacgac gctgcctttc ccctgccgag actcgatgta gccccgcgct ttcaggtagc
     1261 gcagcgcctc gcggatggtg tggcggctga cgtggaagct ctgcgcaagc tccgtctcag
     1321 gcggaagctg gtcgccgggc gagagtttac ccgtctctat cctgccgcgg agatagtcgg
     1381 ctacctggag gtacaaagcc acgcccctct gaggaacgcg tgggaagaag gcccgatccc
     1441 tttctacgcc tggcctttcg aaacgagaga aagccgccac tacctcgcct cactttccgc
     1501 aatcttgctt atctccgact cgaggtatcc gaggacatct ctcagcacgc attcagtgtc
     1561 cccgccgagg gtaggggcgg cattcctggg aactggcgcg ccgtctacct tgatcggaca
     1621 ggagatctcc ttcaccgtgc cgcgccggtc gtgcggcact tctattatca tgtcgcgtgc
     1681 caggacctgc ggctcggcca gggcctcgtc gatcgtgttc acgcgtgcgc agggcacact
     1741 gtcgccgaag gcctctagcc actcttccgt cgtacgctcg aggagtgcct cctgcagctc
     1801 cgggagtaac cactcacggt tctcgtatct ggcggagaag tcgcggtaac ggggttcctg
     1861 cttccactcc ggtcgtctca gcacatcgca gaggttcgcc cagaacttct ctttgttgca
     1921 catgatgtac atgtagccgt tccgggtacg gaaccgctgc gacggtacga gcgacgggtg
     1981 ggcgccgttg ggcgctcgcg acggaagata accctcgttc aggtaccagg ctgcgaggta
     2041 gttgaggttc gagacggcga cgtccaggag gctcacgtcc acgtccatgc cgcgaccgct
     2101 ctcgcgggcg ctgaggaccg ccgacacgat tccgagagcg gcggcgatgc cgctcatgaa
     2161 gtctacgatc gagagcccgc agcggatcgg cgggtcgtcc ggctcgccgg tcacgctcat
     2221 gaacccggcc aacgcctggg ccaggtagtc gtagccgggc tgcgcggcgc gcgggccagt
     2281 gagcccgaag ccggtcagcg aggcggtgac tatcctgggg ttgaccttcc ccagcgcctc
     2341 ataggtgaga cccaaattcg ccggcaggtc tccccggagg ttattcgcta cggcgtcaga
     2401 gaccctcacc aggtcgtgca gcacccggcg tccgaattcg ctacgcaagt ccaggctcac
     2461 acttcgcttg ttgcggttga gcgactggta gaagaggctg tctccatcct cggcgtaggg
     2521 tcccacgaag cggccaatgt cgccgcgcgc cggatcctcg atctttatta cctcggcacc
     2581 caggtctgcg aggtgcatgg tcccgaaggg cagggccccg tactgctcaa cagctataat
     2641 ccggaccccc tctagcggta gcctgggcgt gcaagccatc gtcacccctg tcctccttcg
     2701 ttcagagcgt gccgcttgag cagacccctc gcgatcacca ggcgctgtat ttcgttggtt
     2761 ccctcgccta tggccatcag cggggcatcg cggaagtaac gctccacgtc ataatccttg
     2821 tggtaaccgt taccaccatg aatgcgcatg gcctcgaggc tgttgtcgac gccggcctcg
     2881 ctggcgaaga gcttcgccat acctgcctcg aggtcgcaac gctcgccgcg atcgaacttc
     2941 tcggcggcat tcctgacgag caaacgggca gcctcgatgc ggaccgccat gtcggccagc
     3001 ttcagctgta tggcctgatg ctgggagatg ggctttccga aggcctcgcg ttgctgggcg
     3061 taggcgatcg cgtcctcgaa ggcggcgcga gcgacgccca atccgcgggc agcgacgttg
     3121 atgcgcccca gctccaggcc cttcatgatg tgccggaaac cctttccttc ctcaccgccg
     3181 agcagattat ccgcaggaac ccggaagttc tcgaacacga actcagcgct gtcgatgccc
     3241 ctgtagccta gcttctccgg gcggcgaaac gcctcgaagc cggacccgcc cttctcgacg
     3301 atgaacgcgc tgatgccctt gtatgccggc tccgccagcg gatcggtctt cacggcgacc
     3361 aggaaagtgt ttccgaagat cccgttggag atccacttct tatgtccatt tatgacgtag
     3421 tgatcaccgt ctcgccgcgc ctcggtgcgt atggcctgga cgtcgctgcc cgcgtgagac
     3481 tcggtgatcg acagcccgcc ccgcctctcg ccggaacata gatcgggcag gtatcgatcg
     3541 cgttgttcgg gcgttcccga gaggtagatg tcgtacgcca ggatgagatg gctgttgatg
     3601 ataccggcga tgctcatcca tcccctggag atgtcttcta tgatccgggc gtaggccgag
     3661 acgtccaggc ccagaccgcc atactcctcg gggatgatcg cgccgaacaa cccgagctcc
     3721 ttcatccgct ccacgatccc ctgcgggtat ttgtcctcga cctcgaactc gcggacgaca
     3781 ggctttatct cttcctcgac gaatgcatgc accctttcca acagtgcatg ctgcgccttc
     3841 tcatccatag cctgctcgct cctcacgtgc tgattccaag ccagccccgc agataagccc
     3901 aatgcgacgc gcagtcgaaa gcccggccac gaggcggacc tcgagcctca cgccggacgc
     3961 ggaaggcggc gggcccattt ctctcagccg tgtttcgggt ccactcgaag ggcggacgtc
     4021 aggtcgctga tggaatccag gccatcgagg ttctccacct tacgtgcgac gagttcggcc
     4081 tgctcctccg acaacccgat cgaggtgttc agtcgaaact tctcgcggat ctccgccctg
     4141 ccgagtggtc gttcgggggc accgcgattg atctgctctc gacgggagac cctccgcccg
     4201 tcgcgcaagt ggatgacgac ctcgccggag aagtgcctgg ggaaaccaga atcgggatcg
     4261 acttcgtagc caaccttctc ggcgacaccg agtacccgct cgtctccgat gccctcctcg
     4321 tcgaaatctt tgatgccgag gcggcgtcct cgcacgatgg cactcgcaac cgcgtatggt
     4381 aggctaaatt gcgcgtcgta cgtggtctgc gggcggcgct tggtctttat cggttcgcag
     4441 accgtctcta cctcgccggc ggcgatcttg cagatgatcc gctctacgtc ttccacagcg
     4501 aatctttcct cttgccgcag ctcgagcgcg gcgtctatga aggcgtggtt gaagtgacaa
     4561 gcggggtacg gcttgacggc taccttcagt atctcccaac gctcgcccag atcctccgtt
     4621 gccatctcca gcgggagatc cctggtgccg aggtgggttg cgtacagtcc gaagcgccct
     4681 tcgtagaccg cgcccggtcc ggtgaagccc gctccggcca aactggcggc ggtgaggccc
     4741 gcgtgggcag cccatccagg gtgcaggcgc ttggtccagg taccgtcctc gaggaactcg
     4801 aagatcccgg aggccatgct accgacaatg ccctgagcgg tagcgagttc ctccttgtca
     4861 agtccgaaga gcaaaccggc ggcgacagct gccccaaagg ccccaacgac tccggtcgga
     4921 tggaaacccc acttgtggaa ctcgcctgcg gctaccatcc cgatcctggc ggtcacttcg
     4981 gaacctaata cgaaagcttt gagcacctca ctgccggacc ggtcttcgag ctcacctgcg
     5041 gcgagggcag caggaagggc gctggcgctc gtatggatca cgctagccag atgggtgtca
     5101 tcgaagtcga gcccgtggat caagagcccg ttcatgaggg cggcgctgcc cgtatccacc
     5161 gtccgcaagg aacctatgac ggtggctccc ccctctcccg cgacccgact tatcccggac
     5221 cacgccctct ccgagaacga ctcgctcctg gaggcgaagc cgatccccaa ggcatccagc
     5281 gccagagcct tcgcggtttc gaccacctct gtaggcacgt cctcgtggta cgtgtcgcga
     5341 agaacctcgg cgatttgccc ggatagcgcc gagccacgcc gggaagtggc aaccgcccgc
     5401 ttcaccgtat cgctcatcct atcttccctc ccccataagg gtgtaagtac ggttgtacgt
     5461 acaagtgtcg agatggtaaa cacggctgct gtctgtgtca aggttcgctc tcgaattttg
     5521 agaagctttg aggtactgga gatggagccc tttgactggc cagctacccg cgtatattct
     5581 cggaagcgat cagctggaaa aatcaatgaa cgctggggag actgactgcg aatgactgac
     5641 agaccggatg gtcatgatga atgtgggttg cattgagcca ggcggaaatg cgctacggac
     5701 ttttcgtatc tgcacagtat ccagagaacg ctgatctggg tagaatcctg cacgagcacc
     5761 tcgaacaggt acgtttcgca gctgacaacg gcttcagctc cgtattcgcc ggccaacact
     5821 tcctgtccac tcccttccag atgctacagc cggtgccgct gctggcgcgt ctcgcggcag
     5881 agagcggtga tctgagggtg ggctgcggca tcctccttgc cgccttgctg aacccggttg
     5941 agttggcgga gcaagcggcc acgctcgacg tgatctgtgg cggccgattc gtcctcggtg
     6001 tgggcctggg ctacagggca gaggagaatc tggctttcgg cgtcacctca cgaagagcgc
     6061 gcgttctcga atcgaagctc gacgtagttc gcaggctgct ggagggcgag cgggtaaccg
     6121 ccgagggacc tggctaccag ctcgaggatg cggcaatcgg cgtacgtccc atcgcggaac
     6181 cgcgcccgcc tatctggatg gcggcgaaca gcgatgccgc cgtcaaaagg gcggcgagag
     6241 tcgccgatac atggttcgcc aacccgcacg taactgtaga ggagttgcag agacagctag
     6301 acttcttcag gcaggagcga gggtctgctc cgaccgaact tcccgccatg cgcgaggtgt
     6361 gcgtgaggag gacggatgat gaagccatcg acgttgcccg ccccttcctg gacaacaagt
     6421 acaaatccta tgtctcatgg gggcagagtg aggcactacc gccgaccgat acgttgcgac
     6481 gcaactggga cgaactccgg atgaaccgat tcatcatagg gtctcccgag acggcgatag
     6541 atcaggtccg acaccacagg aacgacctgg gcattgacca tctcgttttt agactgcagt
     6601 ggccgggctt gccgcacatg gaagcgatgg aaacgttgcg gtggctggtt ggagaggtca
     6661 tccctgccgt ctaacccaga gtcgcggccc gacaccttct tcacacctca tcttgcaagg
     6721 ctccaaggat gcagttggag atacttcatc actcgcgtcg gcgacttgcg aggtgaggat
     6781 ggtcgtagtg gtttgaattg cgcgccgtta cgtggcggcc gagcggcaag agcgccgcgt
     6841 aggccgcgag gccgagcagc atcagggcgg cgatcggcgc gtcgtagctg ccggtggcgt
     6901 cgacgacgaa cccgacggcc ggcgggccga gcgccgaacc gaggccgttc gcggtgagga
     6961 gcacgcccag gaggccaccg agccgttcga cgccgaagaa cgcggcgagc accaccgggc
     7021 tcaacgccac gaacccaccg taaccgaccc caagcaccgc ggcaaaggcc acgaggaacc
     7081 agaaaccgtc gccgaaccac cacaacgcga agctcgctcc gatcaggaaa aagctcaccc
     7141 ggtaaccgcg caacggccct gcgctgtcgg cgacggcgcc gagcacgacc cg
//
LOCUS       DATMJA010000054         4349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_303937, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000054 DATMJA010000000
VERSION     DATMJA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303937"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VNT12_01290"
     CDS             complement(<1..426)
                     /locus_tag="VNT12_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWI44773.1"
                     /translation="MAIRETNARHEVLGLDEDDLRRMHYHMLLARRVDERSWILNRQG
                     KAAFVISCQGQEGCQVGAAYNLRPGYDYVYPYYRDHGIVMTLGMTARDELLSLLGKKE
                     DPNSGGRQMPGHFSSRELNIVTASAPISVQYPQATGSALA"
     gene            complement(550..864)
                     /locus_tag="VNT12_01295"
     CDS             complement(550..864)
                     /locus_tag="VNT12_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44774.1"
                     /translation="MEARVVDLRILPTEAREVVRIYRDSVLPVARRQQGFKGALLLTD
                     DETGVGISISLWETEEDREEGEANGFYKQQIRKFSDLLTETPVRKHYNVGVLHYDASV
                     LA"
     gene            complement(1160..2803)
                     /locus_tag="VNT12_01300"
     CDS             complement(1160..2803)
                     /locus_tag="VNT12_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWI44775.1"
                     /translation="MDTRKSAKDRWQEAYSKGPERDAEFSTMSGVPVKPLYTPEDVEG
                     DYDEKLGYPGEYPYTRGVYPNMYRGRLWTVRQFAGYGTAAETNARFRYLLEHGQNGLS
                     VAFDMPTLMGLDSDSPLSLGEVGTEGVAVDSLEDMERLFEGIPMGEVSTSMTINAPAM
                     VLLAMYVVAAEKQGVAPEELEGTNQNDILKEYIAQKEWLFPPEPSMRVFKDMLVYASE
                     HMPKWNTVSISGYHIREAGSTAAQELAFTLADGFAYIEAGIEAGLDVDDFAPRLSFFF
                     NSHIDFFEEICKFRAARRIWATVLKEKYGAKDERSLRMRFHTQTAGVSLTAQEPYNNV
                     ARTAFEALAAILGGTQSLHTNSLDEALALPTEEAVRIAVRTQQIAALETGVANTIDPL
                     GGSYFVEALTDELERQAYDYFRQIDEMGGMIQAIEQGFPMREISDASARYQRELEENK
                     RYMVNVNVYEPQDEQEMELHRIDPEVSEGQVERLKALKERRDNDRVEQCLEELKETAR
                     GEGNTMYPILEAVRAYATVQEISDALKEVFGEYRETPVI"
     gene            complement(2861..4048)
                     /locus_tag="VNT12_01305"
     CDS             complement(2861..4048)
                     /locus_tag="VNT12_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI44776.1"
                     /translation="MNFDLSTDQQEIKDRAAEFADEEVAPRATELDRDDLVPFETLEK
                     LADAGFMGLCVPESYGGVGTDFLSYCLLIEEISRADAGVGVTLAVHTSAGTLPIVMFG
                     TGEQKTRWVSPLARGERIGCFALTEPETGSDASAIQARAEKVEGGYCISGHKQWVTNG
                     RIAGTMILFARAEGGAREGVTAFVVPMDAEGISFGKHAEKMGVISATTDDVHFDNVFV
                     PEEDRLGEEGRGLRVALGTLDTGRIGIAAQAVGIAEAAFRYATQYAAGRTTFGKPIAE
                     HQAIAFKLADMQTKIRAARLLVYEAAWMKGQEMPHGEAGARAKLYASQVANEVAYEAV
                     QVLGGQGYMMDHPVERYYRDARVTEIYEGTSEIQRLVISRAILKGQAEAPGGVESSRP
                     VVG"
BASE COUNT          753 a         1404 c         1302 g          890 t
ORIGIN      
        1 ggccagagcg ctcccggtcg cctgagggta ctggaccgag atcggcgccg aggcggtgac
       61 tatgttgagc tctcggctcg agaagtgccc cggcatctgg cgcccgccgc tgttcgggtc
      121 ctccttcttc ccgaggagcg agagcaactc gtccctggcg gtcatcccca gagtcatcac
      181 gatgccgtgg tcgcggtagt aggggtagac gtagtcgtaa ccgggacgca ggttgtacgc
      241 ggcgccgacc tggcaaccct cctgaccctg gcaggagatg acgaacgccg ctttgccctg
      301 gcggttcagg atccaggacc gctcgtccac cctacgggcg agaagcatgt ggtagtgcat
      361 cctgcgtagg tcgtcctcgt ccaaccccag cacctcgtgg cgggcgttgg tctctcggat
      421 cgccatagac ctcctcctct gctcaaaggt ccccacgcga ggagcggaaa ttactcctcc
      481 gtcactcaaa ttctagcaga ccgaagacgt ccaccgggac gtctgtgcac gttggcctat
      541 acccgtggtc tacgctaaaa cactggcgtc gtagtggaga acgcctacgt tgtagtgctt
      601 gcggaccggt gtctcggtca ggagatccga gaacttcctg atctgctgct tgtagaaacc
      661 gtttgcctct ccttcctccc tgtcctcctc ggtctcccag agcgagatag agatccctac
      721 ccctgtctcg tcatccgtca gtaggagggc tcccttgaac ccctgctgac gtcttgcgac
      781 cggcagtacg gagtccctgt agatgcgaac tacctccctc gcctccgtgg gaagaatccg
      841 aagatccacc accctcgctt ccatgatgcg actccttccg gaaaacccga acatcctgac
      901 acctgagtat caaatggatt atatatcagc atttcagcac gctacggcct gtggcctccg
      961 catgtcagca tttcagcacg ccgccggctt cgccaggcgg cttgtcagca tctcagcaag
     1021 tcgtcccgga gcctccccac cagcgtcgaa cccggacgcc tcagcagcgg tccctcgcgc
     1081 gcggtagagc gaggccggaa ccaagagctg atctgccgac aagcgagggc ctgccaggtc
     1141 cgaagcgtgc tgagaagctc taaatcaccg gcgtctcgcg atattcgccg aagacttcct
     1201 tgagggcgtc ggagatctcc tgcactgtcg cgtaggccct gaccgcctcg aggatagggt
     1261 acatggtgtt gccttcgcca cgggccgttt ccttcagctc ttcgaggcac tgttcgaccc
     1321 tgtcgttatc gcggcgctct ttgagggcct tgaggcgttc gacctgtcct tctgagacct
     1381 cggggtctat gcggtggagc tccatctcct gctcgtcctg gggttcgtag acgttgacgt
     1441 tgaccatgta gcgcttgttc tcttcgagtt cgcgctggta gcgggctgag gcatcagaga
     1501 tctcccgcat cgggaagccc tgctctatcg cctggatcat gccccccatc tcatctattt
     1561 gcctgaagta gtcgtacgcc tgccgctcca actcgtcggt caacgcctcg acgaagtacg
     1621 agccgccaag cgggtctatg gtattcgcca ccccggtctc cagggcggct atctgctgcg
     1681 tccgaacggc gatgcgcacc gcctcctctg tggggagggc gagggcttcg tccagggagt
     1741 tggtgtgcag gctctgcgtc ccgcccagga tggcggcgag tgcctcaaag gcggtgcggg
     1801 cgacgttgtt gtaaggctcc tgggccgtca gagagacgcc cgcggtctgg gtgtggaagc
     1861 gcatccgtag agagcgttca tctttagcgc cgtacttctc tttgagtacg gtcgcccaga
     1921 tacgacgggc ggccctgaat ttgcagatct cctcgaagaa atctatgtgc gagttgaaga
     1981 agaagctgag gcgcggggcg aagtcgtcca cgtctagccc tgcttctata ccggcctcga
     2041 tgtacgcgaa gccgtcggcc agggtgaagg caagttcctg ggccgccgtg ctccccgctt
     2101 cgcgaatgtg gtagccactt atggagacgg tgttccactt gggcatgtgc tccgaggcgt
     2161 agacgagcat gtccttgaag acacgcatcg agggttccgg cgggaagagc cactctttct
     2221 gagcgatgta ctccttgagg atgtcgttct ggttcgtgcc ctccagttcc tccggcgcaa
     2281 cgccctgctt ctcggcggcc acgacgtaca tggcgagcag caccatcgca ggggcgttga
     2341 tggtcatgga ggtcgagacc tcgcccatag ggatgccctc gaagagacgc tccatgtctt
     2401 ccaaggagtc cacggccaca ccctccgtgc caacctcccc gagggagagc ggcgagtccg
     2461 agtcgaggcc catcagggtt ggcatatcaa aggcgacaga gaggccgttc tggccgtgct
     2521 cgaggagata cctgaagcgt gcgttggtct ctgcagccgt cccgtaaccg gcgaactggc
     2581 gtaccgtcca gagccttcca cgatacatgt tggggtacac accgcgggtg tacggatact
     2641 cgcccggata accgagtttc tcgtcgtagt cgccctcgac gtcctcaggc gtgtagaggg
     2701 gtttgaccgg cacgcccgac atggtcgaga actcggcgtc gcgctcgggg cccttcgagt
     2761 aggcttcctg ccagcggtcc ttagcgctct tcctggtatc caaggcgtcc tccttctgcg
     2821 tctctagtcc ctgcgccaag actgtagcat aagcaggtgc ttaccctacg acgggccgtg
     2881 agctctcgac gcccccgggg gcttcggcct gtcccttgag gatggcgcgg gagatgacga
     2941 ggcgttggat ctcactggtc ccctcgtaga tctccgtcac cctggcgtcc ctgtagtaac
     3001 gctcgacggg atggtccatc atgtatccct ggccgccgag aacctggacg gcctcatacg
     3061 ctacctcgtt cgcgacctgt gaggcgtaga gcttggcgcg ggcgcctgct tcgccgtgag
     3121 gcatctcctg gcctttcatc caggcagcct cgtagacgag gagacgggca gccctgatct
     3181 tggtctgcat gtcggccagc ttgaaggcta tcgcctggtg ctcggcgatc ggcttcccga
     3241 acgtggtgcg ccccgcggcg tattgcgtgg cgtagcggaa ggccgcctcg gcaataccga
     3301 cggcctgcgc ggctattccg atcctgcccg tatccaacgt cccaagcgcg acgcgaagcc
     3361 ctctcccctc ctccccgagc cggtcctctt cggggacgaa gacgttatcg aaatggacgt
     3421 cgtccgtggt tgcggagata acgcccatct tctcagcgtg cttgccgaag gagatcccct
     3481 cagcgtccat gggaacgacg aaggccgtaa cgccttctct ggctccgccc tctgcgcggg
     3541 cgaagaggat catggtccct gcgatgcgcc cgtttgtgac ccactgcttg tgccccgaga
     3601 tgcagtagcc gccctcgacc ttctcggccc gcgcttggat cgcagacgcg tccgagcccg
     3661 tctccggttc cgtcagggcg aagcagccga tcctttctcc gcgtgccagc ggcgagaccc
     3721 accgtgtctt ctgctccccg gtgccgaaca tcacgatggg cagcgtgccg gcgctggtgt
     3781 ggactgcgag cgttacgcca accccggcgt cagcgcggct gatctcctcg atcaggagac
     3841 agtaagacag gaagtccgtc cccaccccac cgtacgactc gggcacacat agccccatga
     3901 acccggcgtc ggccagcttc tccaacgtct cgaaagggac caggtcgtcc cggtccaact
     3961 ccgtcgcgcg aggcgctact tcctcatcag cgaactccgc cgcgcgatct ttgatctctt
     4021 gctggtctgt actgagatcg aagttcacga gatcctccta ctccccaacg atcgtcttgt
     4081 accttgtcct ggaccctcgg gtaaccaggc ctacctgagt ctaataggac cttagcacgc
     4141 ttgggaggcc gtgggcgtca gctagagaca cccttccaga gcaggcgaaa gcgagagact
     4201 ctccgattcg gggagcctct tattacgacc ctgaccggaa aaccacgccg gactataatg
     4261 aactttccgg gtagaaggga accgccgagg gaggcgtgcc gatgctggtg aaagacgagc
     4321 ttctggacaa ggtcgtagcc cgaatcagg
//
LOCUS       DATMJA010000055         3757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_303992, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000055 DATMJA010000000
VERSION     DATMJA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303992"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VNT12_01310"
     CDS             <1..459
                     /locus_tag="VNT12_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI44777.1"
                     /translation="AHLATGVSEEDIEVLAQTGAAAAHCPRSNAFLRCGLAPVPAMIA
                     SGIRVGMGTDGLWSSPSMNLFEETLFAVELHGMSGVAGLALATLGGARALGIEAGTGT
                     LEAGKWADLVVVEASQEDGDPERGVLEAAAGGGVAATVVGGNMIYNRLGT"
     gene            482..1126
                     /locus_tag="VNT12_01315"
     CDS             482..1126
                     /locus_tag="VNT12_01315"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI44778.1"
                     /translation="MMMNRDRLSFWVRLVSIVLAAFFLISFIFLGLGTNISYNIFELI
                     GNQDQQQRQADQAPDPQEQIAEAEKALEKDPKDPEKIKDLAALYYNAGRYDDAIQVLQ
                     RGREVAPQDEEIPALIGQIYSQQAQAEKGEKQKELLKKAGDAFAASAEAEPDNADAYL
                     LAGQAYDQAGQPADAIEYYNGYLDLEPKGENAKQVKDRISALLEGGNSSGSAGP"
     gene            1193..1474
                     /locus_tag="VNT12_01320"
     CDS             1193..1474
                     /locus_tag="VNT12_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWI44779.1"
                     /translation="MFGSVGMPELIIGLVVILLLFGAKRVPELARGLGSGVREFKKGA
                     QEGEVEDKKKEEAKKDEEQLEAGKSEPDDETKLEEDASVEADKAEQKHS"
     gene            1558..2388
                     /gene="tatC"
                     /locus_tag="VNT12_01325"
     CDS             1558..2388
                     /gene="tatC"
                     /locus_tag="VNT12_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWI44780.1"
                     /translation="MTLIEHLDELRSRIIKVGIAFFGVSVVAWFFRERIFDWLLAPAG
                     GALQGKLNVTGVTEQLFTDMKLALYVGFLLTIPVLLYQVWAFVAPAVGDMGRAFTYTI
                     ISMASALFIAGVAFGYFIVLPVGTQFLLSWDTERFDPIITAQSYLPFVTRFLLAFGIV
                     FELPAATYVAAKLELVDAPLLKRYRKHAIILNTVLAAALTPGQDPYSMILMAVPMILM
                     YEISILIARYVNPTTEVTAHELVRDDEDEPEDHEADEPEISDFEATDDHPENGHERDL
                     "
     gene            complement(2385..2861)
                     /locus_tag="VNT12_01330"
     CDS             complement(2385..2861)
                     /locus_tag="VNT12_01330"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWI44781.1"
                     /translation="MPEDHRPFRTSYIVSGVTAERIFASLLDVQRFPEWAAGLRRCRA
                     LDPASKAETKDLRPGVRLEFTLSAAGLTHEVVSIVTVVEPPRRLEWNYVEGAIGSGGW
                     LVEQEAPGAVRITVSTDYLIRPTWLDRLAHKPFFRKLTEDLLKRSMRRFDAHLRYD"
     gene            complement(2854..>3757)
                     /locus_tag="VNT12_01335"
     CDS             complement(2854..>3757)
                     /locus_tag="VNT12_01335"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HWI44782.1"
                     /translation="TMNPTRRQVFEEWFRLARPFSLTAAAVPVFFGTALAFKDGSFAW
                     GPFFAMLFGSLLIQAATNMFNEFYDAQRGLDVAQTVGIAGSIVSGRVSARRVLAGALL
                     CYTLALFLGIYLIAVGGWPILALGCLSALGGYLYSAGPRPIAYTPASELAVFVFMGVL
                     IVVIAYAVQARGFPPYVPLAALPIGGPVAAILLANNIRDLVSDRRGGRRTLPVVFGRE
                     VAILVYRALLLEAYVAVAALMAFCVVPPECALVFPSVFAAIRLWRDVASYSTPRRLDP
                     VVKRTAGLHLVFGLLYTAGVLIGA"
BASE COUNT          781 a         1132 c         1233 g          611 t
ORIGIN      
        1 gcgcacctgg cgacgggagt ctcggaggag gacatagagg ttctggcgca gaccggtgcg
       61 gcggcggccc actgcccccg ttccaacgcc ttcctcagat gcggcctcgc gcccgtgcct
      121 gccatgatcg ccagtggcat cagggtcggt atggggacag acgggctctg gagcagcccg
      181 agcatgaacc tcttcgagga gacgctgttc gcggtggagt tgcacggtat gagcggtgtg
      241 gcaggcctcg cgctggcgac gctgggaggg gcgcgggcgc tcggtatcga ggccgggacg
      301 gggacgctcg aagcggggaa gtgggccgac cttgtcgtgg tcgaggcttc gcaggaggac
      361 ggagatcccg aacggggtgt tctcgaagct gccgccggcg ggggcgtcgc ggctaccgtc
      421 gttggcggca acatgatata caatcgtctc ggcacctgaa gcccccggag gatagaccgc
      481 catgatgatg aaccgcgaca gactcagctt ctgggtgagg ttggtgtcca tcgtgctcgc
      541 ggcgttcttt ctcatctcct ttatctttct cggccttggc accaacatca gctacaacat
      601 cttcgagctg atcggcaacc aggaccaaca gcaacggcag gccgaccagg cccccgatcc
      661 gcaggagcag atcgcggaag ccgagaaggc cctggagaag gatcccaaag accccgagaa
      721 gataaaggac ctcgccgccc tctattacaa cgcgggccgc tatgacgatg cgatccaggt
      781 attgcagaga gggcgcgagg tcgccccaca ggacgaagag atcccagccc tcataggtca
      841 gatctacagc cagcaggcgc aggccgagaa gggggagaag cagaaggagc tgctgaagaa
      901 ggcgggggat gccttcgcgg cgtcagcaga ggcggagccg gataacgcgg acgcctacct
      961 tctggccggc caggcctacg accaggcagg ccagcccgcc gacgccatcg agtactacaa
     1021 cggctacctc gacctcgagc cgaagggcga gaacgccaag caggtgaagg acaggatctc
     1081 ggcgctcctc gaaggcggaa acagcagcgg tagcgcaggg ccctgatcca caccaggacg
     1141 gaaggcgtat aatgttgcgt gagatccggc agatagctcg ttaggaggaa aggtgtttgg
     1201 atcggtcgga atgccagagt tgatcatagg tcttgtcgtc atactcctgc tcttcggggc
     1261 caagcgcgtt cccgagcttg cccgcggcct cggcagcggc gtgcgcgagt tcaagaaggg
     1321 cgcgcaggag ggtgaggtcg aagacaagaa gaaagaggaa gcgaagaagg acgaagagca
     1381 gctcgaagca ggcaagagcg agcccgacga cgagaccaag ctggaggaag atgcttccgt
     1441 cgaggccgat aaggccgagc agaagcatag ctagacccct tacgggtacc tgagttccac
     1501 atggccggtc gcctgagact cccttcccgt ccgcgtggtc cgcgcgacga gatgcgcatg
     1561 accttgatcg agcatctcga cgagctgcga tccaggatca tcaaggtcgg tatagcgttc
     1621 ttcggcgtct ccgtcgtcgc ctggtttttc cgggagcgga tcttcgactg gctactcgcc
     1681 cccgcgggcg gggccctaca aggcaagttg aacgttaccg gggttacaga gcagctgttc
     1741 accgacatga agcttgcgct ctacgtaggg ttcctgctca cgatcccggt actcctatac
     1801 caagtgtggg cattcgtggc ccccgcggtc ggcgacatgg gccgggcctt cacttacacc
     1861 ataatcagca tggcctcagc gctgttcatc gccggggtgg ccttcgggta ctttatcgtg
     1921 ctgcctgtcg gcacccagtt cctgctcagc tgggacacgg agcgcttcga tcccatcatc
     1981 acggcccaga gctatctgcc tttcgtgacg aggtttctcc tggccttcgg gatcgtattc
     2041 gagttacccg cggcgacgta tgtagccgca aaactggaac tagtcgacgc tccgctcctc
     2101 aagaggtaca ggaagcacgc catcatcctc aacaccgtcc tcgccgccgc ccttacgcct
     2161 ggacaggacc cctacagcat gatccttatg gccgtcccga tgatattgat gtacgagatc
     2221 tccatcctta tcgcccgcta cgtgaacccg acgacagagg tcacggcgca cgaactggtg
     2281 agagacgatg aggacgagcc cgaagatcac gaggccgatg agcctgagat aagtgacttc
     2341 gaagccacag acgatcaccc cgagaacggg catgagcggg atctctaatc gtacctcagg
     2401 tgcgcgtcga agcggcgcat cgaacgcttg agcaggtcct cggtaagctt gcggaagaag
     2461 ggcttgtggg ccaacctgtc gagccacgtt ggcctgatca ggtaatctgt cgagacggtt
     2521 atcctgactg ccccaggggc ctcctgctcg acgagccagc cgccgcttcc aatggcacct
     2581 tcgacgtagt tccattccag ccggcgtggc ggctcgacca cggtgacgat gctgactacc
     2641 tcgtgcgtga gcccggcggc gctcagggta aactccagcc tgacgccggg acgtaggtct
     2701 ttcgtctccg ctttgctggc agggtcaagc gcgcggcagc gtcggaggcc ggccgcccac
     2761 tcggggaagc gttgcacgtc gaggagggag gcgaagatcc tctccgctgt aacgccggat
     2821 acgatatagc tggtcctgaa aggacggtgg tcttcaggca ccgatgagga cccccgccgt
     2881 gtagagcagt ccgaagacga ggtggagtcc ggccgtccgc ttgaccacgg gatcgaggcg
     2941 cctcggcgtc gagtaactgg ctacgtcgcg ccagaggcgg atggcggcga acacgctcgg
     3001 gaagacgagg gcgcactcag gggggacgac gcagaaggcc atcagcgcgg cgaccgcgac
     3061 gtaagcttcg agcaagagtg cccggtatac gaggatcgcc acctccctgc cgaagactac
     3121 gggcagcgtc cgccgcccgc ctctacggtc ggagacgaga tccctgatgt tgttggcgag
     3181 caggatagcg gccacgggcc caccaattgg cagcgccgcc agcggcacat acggagggaa
     3241 accacgagcc tgcacggcgt aggcgatgac gacgatcaaa acacccataa agacgaacac
     3301 cgcaagctcg ctggcggggg tgtatgcgat agggcgcggt ccggccgagt agaggtagcc
     3361 gccgagcgcc gagaggcacc cgagtgcgag tatgggccag ccgcccacgg ctatcaggta
     3421 gatgccgaga aacaacgcca gggtgtagca aagcagcgca cccgcaagga cgcggcgcgc
     3481 actcacgcgg ccacttacta tagagccggc gataccgacc gtctgggcta cgtcgaggcc
     3541 gcgctgcgcg tcgtagaact cattgaacat gttggtcgcc gcctgtatga ggaggcttcc
     3601 gaagagcatg gcgaagaaag gtccccaggc gaacgagccg tccttgaacg ccagcgcggt
     3661 cccgaagaat acaggcacag ccgccgcagt taggctgaaa ggcctggcaa ggcggaacca
     3721 ctcctcgaaa acctgccgtc tcgtagggtt catcgtt
//
LOCUS       DATMJA010000056         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_67398, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000056 DATMJA010000000
VERSION     DATMJA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67398"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1177
                     /locus_tag="VNT12_01340"
     CDS             83..1177
                     /locus_tag="VNT12_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI44783.1"
                     /translation="MQPQRDLRDIEEIQELFEAGQQTGTLESSEVLDLLQEVDLSTDE
                     IQQVYGLLREHGVEVVDAGFLAETMGEDEDTQLVKEVLEGDLKSRYTGDAVQMYLDEI
                     GKTPLLTKAQETYLARRIERGDRRAKAHLTKANLRLVVSIAKKYAGRGVSLLDLIQEG
                     NIGLMRAVEKFDYRKGFKFSTYATWWIRQAVTRAIADKGRTIRVPVHMVEKINKYYRV
                     QRGLGAQLNRDPTDEEVASIMEVDAEEIVRIRRVSRRSISLETPVGEDHSSELGDFIA
                     DEESESPHDLAKSSLLKARMREALGSLPERERMVLEYRFGLTGGQPKTLEEVGERFDV
                     TRERIRQIQLTALAKIKSSPHAAGLRDLLE"
     gene            complement(1202..1354)
                     /locus_tag="VNT12_01345"
     CDS             complement(1202..1354)
                     /locus_tag="VNT12_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44784.1"
                     /translation="MSIAIVSLLVLVIVVIVAIPILIRVFGEAARRNPNEEENESPEE
                     DSNRDS"
     gene            complement(1611..2078)
                     /locus_tag="VNT12_01350"
     CDS             complement(1611..2078)
                     /locus_tag="VNT12_01350"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWI44785.1"
                     /translation="MAQRVNEGIEVDAPVDDVFRYWSNFENFPTFMSNVEVVRMAAQD
                     TSHWRVKGPLGKSVEFDARTTEMAPNRGIGWNTVDGEVMTSGEVRFEETMPGRTQVDV
                     TMNYADPPGGMVGEAVTNILSNPQRNLKEDLQNFARIVERGELGGPEAQTPSR"
     gene            complement(2150..>2922)
                     /locus_tag="VNT12_01355"
     CDS             complement(2150..>2922)
                     /locus_tag="VNT12_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWI44786.1"
                     /translation="RGDEVSEVVEKVKGPEGTSVELTVRHDGERRTYDLQRAEIDSPV
                     ASWTLIPGTDVGLVLLSSFSDASAQELQNAFEEAKAAGARRFILDLRNNPGGRLDQAV
                     QMAGYFLEPESVVYIRKDASGEREEITVEGDPESTEAPLAVLVNGGSASSAEILAGAL
                     RDNDRAPVVGETTFGTGTVLSEFVLSDGSSILLGIAEWLTPDGDFIRNTGITPDVRVP
                     LSEGTEPLTPDDVRDLSRNEIFEKDAQLRTAYEKLQSQ"
BASE COUNT          570 a          896 c          874 g          582 t
ORIGIN      
        1 agcaaattga ttattgtctc ttttaagata tattacgtga aggctacagg agggtttcaa
       61 cctgaagttc ccggagtaga ttttgcagcc tcaacgtgac ctgcgggaca tagaggagat
      121 acaggagctg ttcgaggccg gtcagcagac cggcacgctg gagtcgagtg aagtactcga
      181 cctcctacaa gaggtcgacc tctcgaccga cgagatccaa caggtctacg ggcttctccg
      241 cgagcacggc gtcgaggtgg tcgacgccgg attcctcgcg gagacgatgg gcgaagatga
      301 ggacacccag ctcgtcaaag aggtgctcga aggcgacctg aagagccgct acacggggga
      361 cgcggtccag atgtacctgg acgagatcgg caagacccca cttcttacca aagcccagga
      421 gacttacctc gcgcggcgca tagagcgcgg cgacaggcgc gccaaggccc atctcaccaa
      481 agccaacctc aggctcgtcg tctccatagc caagaagtat gctggaaggg gcgtctcgct
      541 gctcgatctg atccaggagg gcaacatcgg gctgatgcgg gccgtcgaga agttcgacta
      601 ccgcaagggc ttcaagttct cgacctatgc cacgtggtgg attcgccagg ccgtcaccag
      661 ggccatagcc gacaagggac gtacgatccg ggtccccgtc cacatggtcg agaagatcaa
      721 taagtactac agggtccagc gcggcctcgg cgcccaacta aaccgcgacc ccacggacga
      781 agaggttgca agtatcatgg aggtcgacgc tgaggagatc gtgcgcatca ggcgcgtatc
      841 gaggcgttcg atctccctgg agaccccagt aggggaggac cactcctcgg agctgggtga
      901 ctttatagcg gatgaggagt cggaatctcc ccacgacctc gccaagtcct cccttttgaa
      961 ggcccgcatg cgcgaagcgc tcggtagcct gcctgaacgt gagcggatgg tcctcgagta
     1021 ccgcttcggc cttaccggcg gccagcccaa gaccctcgaa gaagtcggag aacgtttcga
     1081 cgtcacccgc gagcgtatcc gtcagataca gcttacagcc ctggccaaga taaagagcag
     1141 cccccacgcc gccggactgc gcgatctgtt ggaatagagc tagtcagccg gagcgtggtg
     1201 actagctgtc tcggttggaa tcttcctctg ggctctcgtt ttcttcttcg ttcgggttgc
     1261 ggcgggcggc ctcaccaaag acgcggatca gtatgggtat ggcgacgatc acgacgatca
     1321 cgagaacgag cagagagact atggcgatgg acacgagatt gttccttccc gtgagtggtg
     1381 tatagaggcc cccgggtttt acccatccgg cgacctctat actatcctac aacagtgtcc
     1441 tcggtaacca cagcacgcta cggcctttgg ccctcgcttg tcagcttgtc agccgaagct
     1501 tgcgcacgtg agggctgact agctgaagtg ctgaaatgct gacaagcgcc cgacagggcg
     1561 tgtgctgaaa tgctcgtcgt gcataaggct caatagcctt atgcacgacg ctagcggctt
     1621 ggcgtctggg cctccggccc gccgagctct ccgcgctcga cgatcctggc gaagttctgc
     1681 aggtcctcct tcaggttccg ctgtggattg gagaggatgt tcgtgacggc ctcgccgacc
     1741 atcccgccgg gcgggtcagc gtagttcatc gtcacgtcta cctgcgtacg ccccggcatg
     1801 gtctcttcga agcggacctc gccgctggtc atgacctcgc cgtccaccgt gttccagcct
     1861 atgccgcggt tgggagccat ctcagtcgtc ctcgcgtcga actctacgct cttgccgaga
     1921 ggacctttga cccgccaatg actggtgtcc tgggcagcca tcctgaccac ttcgacgttg
     1981 gacatgaagg tcgggaagtt ctcgaagttg gaccagtaac ggaagacgtc atcgacgggg
     2041 gcatctacct cgatgccctc gtttactcgc tgcgccatgg gcatcctttc tctcggctac
     2101 gaggcacatc ttgcccaaga agatgcccct tccaaacccg ctcacctcgc tactgactct
     2161 gtagcttttc gtaggctgtg cgcagttggg cgtccttctc gaagatctca ttccgggaga
     2221 ggtcgcggac gtcgtcgggc gtaagcggtt ctgttccttc ggagaggggc actcttacgt
     2281 ctggtgtgat ccctgtattg cggataaagt cgccgtccgg ggtaagccac tcggcgatac
     2341 ctagcaggat agatgagccg tcactcagga cgaactcaga gagcacggtg cccgtgccga
     2401 aggtggtttc cccgacgacg ggcgcgcggt cgttgtcccg caaggctcct gccagtatct
     2461 cagcgctcga ggcagaaccc ccgttgacca gtacagcgag cggcgcttcc gtcgattccg
     2521 ggtcgccttc gactgtgatc tcctcgcgct cgccggaagc gtccttgcgg atatagacga
     2581 cgctctccgg ctcgaggaaa tatcccgcca tctgcaccgc ctggtcgagc ctgccgccgg
     2641 gattgttcct gagatcgagg ataaagcgcc tggcccctgc cgccttcgcc tcctcgaaag
     2701 cgttctgcag ttcctgggcg ctggcatcgg agaacgacga gaggagcacg aggccaacat
     2761 cggtgccggg gataagggtc cacgaagcta cgggcgagtc tatctccgca cgctggaggt
     2821 cgtacgtacg cctctcgccg tcgtgccgga cggtcaactc gacgctggtt ccctccggtc
     2881 ccttgacctt ctccacgacc tccgagactt cgtcacccct ga
//
LOCUS       DATMJA010000057         3957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_304627, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000057 DATMJA010000000
VERSION     DATMJA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304627"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..495)
                     /gene="tsaE"
                     /locus_tag="VNT12_01360"
     CDS             complement(73..495)
                     /gene="tsaE"
                     /locus_tag="VNT12_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWI44787.1"
                     /translation="MLEELASRVAQVLGPGDVVVLSGEVGTGKTTFVRAAARSLGVTG
                     RVTSPTYQLARGYEGLKDGRRVTVNHMDLYRLEGIEDRDVLELEDYLEPDAVTFIEWA
                     DSALGVIENPSIVSIYHETPTTRRISLRGPAAERLATC"
     gene            complement(539..1171)
                     /locus_tag="VNT12_01365"
     CDS             complement(539..1171)
                     /locus_tag="VNT12_01365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWI44788.1"
                     /translation="MDFAEAQKEALGCTKCGLCHSRTQVIFGEGPLNAGLFIVGEAPG
                     FNEDKEGKPFSGASGILLDRMLESLDLGREKVYLTTLVKCRPPGSPPRPPKPSEVSSC
                     RPYLVSQLAAVDPKVVVALGDLPSRVLTGRKESVSRIRGRAIPLDGRYVFPTLSLQRA
                     LYTPADRALLVSDFSRLPELLEAERPSTYETLNVTRSPDAQREPVQPGLW"
     gene            complement(1176..1376)
                     /locus_tag="VNT12_01370"
     CDS             complement(1176..1376)
                     /locus_tag="VNT12_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWI44789.1"
                     /translation="MNIKRAKRDRWIRGVCGGVAHTYGWSSNVVRLVTVVIAVIIPGP
                     STLLAFVIYLLLGFILPESEEF"
     gene            complement(1552..1899)
                     /locus_tag="VNT12_01375"
     CDS             complement(1552..1899)
                     /locus_tag="VNT12_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44790.1"
                     /translation="MFEDLNWKSAARRSAVVIAIYLGLLYLLSLVFPDSNFNMDTRAQ
                     VISLFVNAIIFFFIFTFVYALVERSRNRRIAESRKQKKPGKQATGDQEADAPSLKGRP
                     NPNTSRKKTRRRR"
     gene            complement(1883..2998)
                     /gene="alr"
                     /locus_tag="VNT12_01380"
     CDS             complement(1883..2998)
                     /gene="alr"
                     /locus_tag="VNT12_01380"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWI44791.1"
                     /translation="MSSTAAGVRASSRTWAEVDLGAVRHNVGTLKRRAPNSRVMAVVK
                     ADAYGHGSVEVARACVEAGADSLAVVTVEEGAELRRAGMQALILVFTDLPPDRLRLAG
                     ELRLAVTAHSIPSARRIAAYPGLEAHLKVNTGMNRWGVEPWEVGEARKILGPRLAGVY
                     THFASADSDQEATRGQIERFESVLASQPFGGVLVHAANSAATLWHPGSHYDCVRPGVA
                     LYGLHPAGDEGDPAAEGLRPAMLLKSYVAGVRHLARGDGVSYGLTYRAGAPMFAATVP
                     VGYAEGYSRALSGRAEALIRGQRRPLLGRVTMDACVFGVDEDVEVGNEVVLLGEQGEE
                     RITAEELGSRAGTINYEITTGIDPRRVERSYEDVRGS"
     gene            complement(3008..3487)
                     /locus_tag="VNT12_01385"
     CDS             complement(3008..3487)
                     /locus_tag="VNT12_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWI44792.1"
                     /translation="MEQQDIRDLRVADISHAEWPTLGPDETVEGAIKLFAESDISGAP
                     VVDNDRLVGIVTEGDLIFRDADIKAPGFLDILGGLIPLGSWDEYRKEALKSAGVTVDE
                     VMTRELITISPDASLAEASTMMADKRVKILPVAEEDILHSVITRMDILTLHVMNPHR"
     gene            complement(3499..>3957)
                     /locus_tag="VNT12_01390"
     CDS             complement(3499..>3957)
                     /locus_tag="VNT12_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HWI44793.1"
                     /translation="VSPFEAADLAVEELEARGAELVLVDSHAEATSEKQALGYYLDGR
                     AHAVLGTHTHVPTADYSILPGGTAYVTDVGMTGCTESIIGFERDDFLALFLGDWRGIS
                     VATRGPVVLNGALVEFDLDARRATGIEAVSREWKPWPEARRAVYNSGNDD"
BASE COUNT          711 a         1293 c         1235 g          718 t
ORIGIN      
        1 cgagcacctc gcggccgttt tcgcgggcga gggcgacggt ggttaccggg gtcgatgcgt
       61 cgagggctag gatcagcaag tcgctagacg ctccgcggcc ggtcctctga ggctgatgcg
      121 ccgcgtggtc ggggtctcgt ggtagatgct gacgatggag gggttctcga taacgccgag
      181 tgcagaatct gcccactcga taaacgtcac ggcatcaggt tccaggtagt cctccagttc
      241 gagtacatcc cggtcttcga tcccctccag gcggtagagg tccatgtgat tgacggtcac
      301 ccgtcgcccg tccttgagcc cctcgtagcc gcgggcgagc tggtaggtcg ggctcgtcac
      361 cctgcccgtg acgccaagag atctggcagc agcccgcacg aaggtagtct tgccggtgcc
      421 tacctcgccg gagagcacca cgacgtcacc aggacctaga acctgcgcta ccctggatgc
      481 cagctcctcg agcatagctt cctttaagcc ttcgagctcc acggggaacc ctgggactct
      541 accagagccc cggctggact ggttcccgct gcgcgtcggg ggagcgggtg acgttgaggg
      601 tctcgtaggt ggagggccgc tcggcctcga ggagctcggg caggcgcgag aaatcagaga
      661 cgaggagcgc ccggtccgcc ggagtgtaga gggcgcgttg gagagagagc gtcgggaaga
      721 cgtagcggcc atcgagcggt atcgcacgcc ccctgatgcg cgagacggac tctttcctcc
      781 ccgtgagtac ccggctgggc aaatccccga gcgcaacaac gaccttgggg tcgacggccg
      841 cgagctgaga gacgaggtaa ggccggcagg agctcacctc agagggctta ggcggccgtg
      901 gtggagatcc aggcgggcgg cacttgacaa gcgtagtcag gtagaccttc tcgcgcccga
      961 ggtcgagaga ctcgagcatg cgatctaaga gaatccccga ggcccccgaa aagggtttgc
     1021 cctccttgtc ctcgttgaag cctggtgcct caccgactat gaataatccg gcgttcagcg
     1081 gcccctcgcc gaagatgacc tgtgtcctgg aatggcacag cccgcacttc gtgcagccca
     1141 gcgcctcctt ctgcgcctcc gcaaagtcca agcgcctaga actcctcgct ctcagggagg
     1201 atgaagccca acaacaaata gatcacgaaa gccagcagcg tgctcggacc ggggatgatc
     1261 acggcgatca ccaccgtaac aagccgcact acattggagc tccaaccgta agtgtgggcc
     1321 acgccgccgc agacgccccg gatccaacgg tccctcttcg ccctctttat gttcaagatc
     1381 tcaaacctcc ccggccccag cgaagccgct ccgctctcgg cgcgattcta cacgaccacc
     1441 gccgggatca tctacaggaa ggtggcgagc agctagatag gtaccgggga tgacgcagag
     1501 gagcccgacg gtatccagcg ggggttccag ggccgcgcgg aggtccaacg gttagcgtcg
     1561 gcggcgggtc ttcttgcgac tcgtattggg gttgggacgc cctttgagcg aaggggcgtc
     1621 ggcctcctgg tcgcctgtgg cctgctttcc cggcttcttc tgtttacggg actcggctat
     1681 gcgcctattt ctactgcgtt ccacgagcgc gtagacgaac gtgaagataa agaagaatat
     1741 gatggcgttc acgaacaggc tgatgacctg agcccgagtg tccatgttga agttgctgtc
     1801 gggaaacacc aggctaagga ggtagagcag accgaggtag atcgctatca ctaccgccga
     1861 cctcctggcg gctgacttcc agttaagatc ctcgaacatc ttcgtagctc ctctcgacgc
     1921 ggcgggggtc tatccccgtg gtgatctcgt agtttatcgt gcctgcgcgg gagccgagtt
     1981 cttcggctgt aatgcgctct tctccctgtt cgccgagcag cacgacctcg ttcccgacct
     2041 cgacatcttc gtccaccccg aagacgcagg catccatcgt gacccgcccc agaagcgggc
     2101 gcctctggcc ccggatcagg gcctcagccc ttcccgaaag cgcccgacta tacccctccg
     2161 cgtaccctac aggcacggtc gccgcgaaca tcggcgcccc ggcccggtag gtcagcccgt
     2221 aggatacccc gtcgcccctg gcgaggtgcc gcacgccggc aacgtagctc ttgaggagca
     2281 tcgcaggccg tagaccctcc gccgccggat ctccctcgtc gcccgcaggg tgcaaaccgt
     2341 agagcgccac gccgggacgg acgcaatcgt agtgcgaccc tggatgccag agggtggccg
     2401 cagagtttgc agcgtgcacg agaacgccgc cgaaaggttg cgaggcgagc acggactcga
     2461 agcgttcgat ctgacctctc gttgcctcct ggtctgagtc ggccgaggcg aaatgcgtgt
     2521 agacgccggc gagcctcggg ccgagtatct tgcgcgcctc tcccacttcc cagggctcta
     2581 ctccccagcg gttcatcccc gtgttcactt tcaggtgcgc ttcgagacct gggtaggccg
     2641 cgatgcgcct cgcgctcggg atggagtggg ccgtcacggc aaggcgaagt tccccggcta
     2701 gccgtagcct gtccggcggg agatcggtga agaccaggat cagggcttgc atacctgcgc
     2761 gccgcaactc cgccccttcc tcgacggtga cgacggcgag agagtccgcc ccggcctcga
     2821 cacaggcgcg ggccacctcg acggagccat gtccgtaagc gtccgccttg acgacggcca
     2881 tcacgcgcga attcggggcc ctgcgtttga gggtgcccac attgtgacgc acggcgccaa
     2941 ggtccacctc cgcccaggta cggctcgagg ccctgactcc ggcggccgtg ctactcaccg
     3001 tccgttctta ccggtggggg ttcattacat ggagggtcag gatatccatg cgggtgatca
     3061 cactgtgaag gatgtcctct tcggcgacgg gtaggatctt gacccttttg tcggccatca
     3121 tggttgaggc ctcggcgaga gaggcgtcgg gggagatcgt gatcagctcc ctggtcatca
     3181 cctcgtcgac ggtaacgccg gcgcttttga gcgcttcttt gcgatactcg tcccaactcc
     3241 ccagtgggat gaggccgccg agtatgtcga ggaagcccgg cgcctttata tccgcgtcgc
     3301 ggaagatcag atccccctcc gtgactatgc cgacaagtct gtcgttatcg acgacagggg
     3361 cgcccgagat atcagactcg gcaaagagct tgatcgcacc ttccaccgtc tcgtcgggcc
     3421 cgagcgtggg ccactccgcg tgcgagatgt cggccaccct caaatcccta atatcctgct
     3481 gttccatgtt ccaccccact aatcgtcgtt cccgctattg taaacggccc ggcgagcctc
     3541 cggccaaggc ttccactccc tgcttacagc ttcgatcccc gtggcccgcc gagcgtcgag
     3601 gtcgaactcc acaagcgcgc cgttgagaac gacaggaccg cgggtggcga ccgagatccc
     3661 acgccaatca cccaggaaca aggcgaggaa gtcatcccgt tcgaagccta taatgctctc
     3721 cgtgcagccg gtcatgccga cgtcagtcac gtacgcagtt ccgccgggca ggattgaata
     3781 gtccgccgtg ggcacgtgcg tatgggtgcc caatacagcg tgcgccctgc cgtcgagata
     3841 atagccgagc gcttgcttct cgctcgtggc ctcggcgtgg gagtcgacga gcacgagctc
     3901 ggcgccgcgc gcctctaact cctcgacggc caggtcggca gcctcgaagg gcgatac
//
LOCUS       DATMJA010000058         3079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_245394, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000058 DATMJA010000000
VERSION     DATMJA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245394"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VNT12_01395"
     CDS             complement(<1..418)
                     /locus_tag="VNT12_01395"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWI44794.1"
                     /translation="MTQPLIAVTMGDPAGIGPEIIARAFDEEDFRKHNRAIVVGDAGI
                     LERAARLLDLALRANVVAEPEDARFEPGTVDVISETELPEDLPFGELDARAGDAAFRY
                     VERAAGLASERRVGAMATAPLNKEAMHLAGHKYPGHT"
     gene            complement(415..1608)
                     /locus_tag="VNT12_01400"
     CDS             complement(415..1608)
                     /locus_tag="VNT12_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HWI44795.1"
                     /translation="MLIAVIADDLTGAADTGVQFVHAGYRAAVFFRATEMLADDLDAV
                     VFDTDSRAMPAGFAAKRVVDAAHVARGARIVYKKLDSTLRGNVAAELAAALGGAHRGR
                     AVVAPAFPAAGRTTVGGIQRVHGVPVDETEMANDPRTPVSEAHIPSLLADAFSSVGAL
                     GVEDLADPDLVLRALEDYECVVTDAERDADLEAVVRAVPDPARVLWAGSAGLALALGS
                     VYPGPCAGDAGVHRAPVRPVLVVVGSLSDVAREQVRRLVEAYGEVDVPVHGKKPNTVQ
                     KAVGTAREALAGGTCAVVRSPDERVASSESVLGDLAEVAALLSEAGLFEGLVLTGGAT
                     AVGVARRLGASGIRLEGEVETGVPMGVLVGPRPYPVVTKAGGFGGPDTLEGAVEALSA
                     KERNA"
     gene            complement(1791..2564)
                     /locus_tag="VNT12_01405"
     CDS             complement(1791..2564)
                     /locus_tag="VNT12_01405"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI44796.1"
                     /translation="MERAARGLGRLRGKVAVVTGAAKGIGRATADLFSREGARLVITD
                     VDGAGLSRLRERLAGSGFEVEAVVGDVSVVEDARRMISTAVETLGRIDVLVANAGIIP
                     LNTIVEATPEDWDRVMAVDGRGMFLTCKFAVEAMLGQEEPGGSIVCLSSISGLAGQAQ
                     QSTYGPAKFVASGITKHLAVEWAARGIRVNAVAPGTIATEALVELPEEYIAPIREAHP
                     VGRLGEPAEVANAILFLASDEASFVTGAILPVDGGYLAR"
BASE COUNT          581 a         1067 c          886 g          545 t
ORIGIN      
        1 cggtgtgccc gggatacttg tggcctgcca ggtgcatggc ctccttgttc agcggcgcgg
       61 tcgccatcgc cccgacgcgc cgctcgctcg ccagccccgc cgcccgctcg acgtagcgga
      121 aggccgcgtc gccggccctt gcgtccagct cgccgaaggg gagatcctca ggcagttcgg
      181 tctcggagat cacatctacg gtgccaggct cgaacctcgc gtcctcgggt tcagcgacga
      241 cgttcgcgcg cagcgccagg tccagcaggc gcgcagcccg ctccagaatc ccagcgtcgc
      301 ctaccacgat ggccctgttg tgttttcgga agtcttcttc gtcaaaggct cgggctatga
      361 tctcaggccc gatcccggcc gggtccccca tcgtcacggc tatgaggggc tgtgtcatgc
      421 gttcctctcc ttcgcgctga gcgcctctac tgctccttca agtgtatccg gtccgccgaa
      481 gcctcctgcc ttggtgacaa cggggtaagg cctgggtccg accaagacgc ccatcggaac
      541 ccccgtctcc acctctcctt cgagcctgat cccagaagcc ccgagccgcc gcgcaacccc
      601 aacggccgtt gctcccccgg tcaagaccag cccctcgaat agccccgctt ccgagagaag
      661 cgcggccacc tcggcaaggt ccccgaggac cgattcgctg gaagcgactc tctcatcagg
      721 cgagcgaacg accgcgcacg ttccaccggc gagcgcctcc ctggcagtcc ctacagcctt
      781 ttgcacagtg ttgggctttt taccatggac agggacatct acctccccgt acgcttcgac
      841 cagccgcctt acctgttccc gcgccacgtc gctcaggcta ccgacaacca cgaggacagg
      901 acggacaggg gctctatgta cgcccgcatc tcccgcgcaa ggcccagggt agacgcttcc
      961 cagcgcgagc gcgagcccgg cagagccggc ccagagtacc ctggcggggt cgggcacggc
     1021 ccgcacaaca gcctccaggt cagcatcgcg ctcagcgtcg gtgacgacgc actcgtaatc
     1081 ttccagagct ctgaggacga gatcagggtc agcgagatcc tcaacgccca atgcgccgac
     1141 cgatgagaac gcgtcagcga gcagactcgg gatatgggcc tcactaacgg gagtacgggg
     1201 atcattcgcc atctcggtct cgtcgacggg gacgccgtgg acgcgctgga tcccaccgac
     1261 ggtggtacgc ccagcggccg gaaacgcggg agcgacgacg gcacgcccgc gatgcgcacc
     1321 acccaacgct gcggcaagct ctgcggcgac attcccccga agggtagaat cgagcttctt
     1381 gtacacgatg cgtgctccgc gagcgacgtg tgcggcatcc acaacgcgct tcgcggcgaa
     1441 gcctgctggc atggcgcggg agtcggtatc gaagactacg gcatccaggt catctgccaa
     1501 catctctgtt gcacggaaaa acacagcggc ccggtaaccg gcgtggacga actggacccc
     1561 ggtgtcagcg gcgccggtaa gatcgtcggc gattacagcg atcagcatgt cagcatttca
     1621 gcttctcagc acgctccggc ctttcaggcc tccgcttgtc agcatgtcag catttcaggt
     1681 ttcgtggtcg gagaagactc tcaatgagta catgctcctc tgacattatc tcaagcgcaa
     1741 gggagcctca ccacactaca catagcttac tggctgacat gctgaattgc tcaccgcgcc
     1801 aaatacccac cgtccacggg gaggatcgcg cccgttacga acgaggcctc gtcggaagcg
     1861 aggaagagta tggcgttcgc cacctctgca ggttcgccga gcctccctac cgggtgagcc
     1921 tccctaatcg gggctatgta ctcctcgggc agttccacga gggcctctgt cgctatcgtg
     1981 cctggcgcta cggcgttgac gcggatgccc cttgcggccc actcgacggc gaggtgcttg
     2041 gtgatccctg aggcaacgaa cttggctggc ccgtaggtag actgctgggc ctgcccggcg
     2101 agacccgaga tcgaggacag gcagactatg gaacctcccg gctcctcctg cccgagcatg
     2161 gcctcgacgg cgaacttgca ggtgaggaac atgccccgcc cgtccacggc catcacacgg
     2221 tcccagtcct caggtgtcgc ctccacgatg gtattcagcg gaatgatgcc ggcgttggcc
     2281 acgagcacgt ctatgcgccc caaggtctcg acggccgtac ttatcatgcg tcgcgcgtcc
     2341 tccacgacag agacgtctcc gacgacagcc tcgacctcga atccagaccc ggccagccgc
     2401 tccctcagcc gcgagagacc ggccccgtcc acatcggtga tgacgaggcg cgctccttcc
     2461 ctggagaaga gatcggcggt cgcccttccg ataccctttg cggcccctgt taccacagcc
     2521 accttcccac gcagcctgcc gagtccccgc gccgctcgct ccatcacgct acctcctccg
     2581 tcataccagc atcaggcacg gctaccctgg aattcttggc actgattaca ctttccgaac
     2641 ccaacctatc accgtcttaa gcgggccgtc aaactcgcgc ggcgcgcctc cttccttcgt
     2701 taaagtcgtg ccaattccag cctgtcggcg ctaccctcgg tgagcaagat gcgcagtgca
     2761 aggcggattg gtttgcatac tattggcgat gtagtgtctt tgcctgagcg aagcaactcg
     2821 acgaagcgca accattgggg tatagagtcg gacgagataa cgcccgggcg actccgtctc
     2881 tacgagagca gcccgagcag gtaggctatc gctagcagaa cgcagaagat caccagacca
     2941 agaaggatgg taagccggag ccgtttgaac actccggcta ctccatctcc acagacaggg
     3001 cccgatcctt cttacgtcag attatgcaaa tgcagacggt ccctgggtga caaacaggta
     3061 gagtatgtag accacgaag
//
LOCUS       DATMJA010000059         5347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_186257, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000059 DATMJA010000000
VERSION     DATMJA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186257"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1695)
                     /gene="ptsP"
                     /locus_tag="VNT12_01410"
     CDS             complement(76..1695)
                     /gene="ptsP"
                     /locus_tag="VNT12_01410"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWI44797.1"
                     /translation="MKLTGIGASEGVAVGPAFVPATGKHEPERDRITEEEVEAELQRF
                     KAAAETVAANLSETGDRLRTAGSEEEAGIFDFHVEMAEDPELASAVEERVVNLESPEA
                     AVLAVGEEYAAEYAAMDDEYMAARADDVRDVTGQISAQLMGRGLSAFEALKTPSVVLS
                     HSLTPSDTARIQKGMALGFVTAEGSRTSHVSIMARSMGIPAVVGVGPALERILGAETV
                     AVDGGEGYAIADPDAETVAEFERMSGALAEERAALDVYRHIEASTSEGRRIEVAANLG
                     SASEAEGALQWGAEGVGLFRTEFLFMERDDLPSEDEQYEAYSAVARAFGEKPVIVRTL
                     DVGGDKDLPGVDQAAEENPFLGWRGIRMSLDTPELFEPQLRAILRAAANGNLKVMFPM
                     VADVGELEAAKETLQECRRELEWEGVEIGEVETGVMIETPAAAVCAAKLAEESAFFSI
                     GTNDLVQYTLAADRGNERLRRLQSADHPAVLALIRQTCVAAGAAGIPVGVCGEAAGEP
                     EMIPKLVELGVTELSMAAPQIPRAKKVVSEL"
     gene            complement(1702..1959)
                     /locus_tag="VNT12_01415"
     CDS             complement(1702..1959)
                     /locus_tag="VNT12_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWI44798.1"
                     /translation="MAQREATVGPEEGIHGRPAARFVKAAKGFSSQVVIVKGDREVNA
                     KSVLKITGLARKGEKVTIRAEGEDAEQAVETLAELIAADEH"
     gene            complement(2016..2777)
                     /locus_tag="VNT12_01420"
     CDS             complement(2016..2777)
                     /locus_tag="VNT12_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWI44799.1"
                     /translation="MRENRLKKIWREGETAVNGWLQVPSSFSAEVMAHSGWDSLTIDM
                     QHGPVDYGSLVPMLQAISTTDTVPVVRVPWNDPGLIMRVLDAGCYAVICPMINTREEA
                     EAFVGACRYPPEGYRSYGPYRATLYGGQDYTDYANETMVTMAMIETHEALENLEQILS
                     VPGLDAVFVGPSDLGQSLGHGPGTDRSEPEVVEAIDRVLAAAREHGLAAGVFTGSPEY
                     ASRMAGKGFQFVTISSDVRLMASAAAGAIAAFESE"
     gene            complement(2743..3060)
                     /locus_tag="VNT12_01425"
     CDS             complement(2743..3060)
                     /locus_tag="VNT12_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44800.1"
                     /translation="MRIAAVAEVLAACWSWEDSGWVWDEALTFAELLAGIKGWAGEAA
                     DENTADALVSSQTRQGVGQRTGITCDVEGLAATSTGLQDASVWRWRRRSKDEGESSEE
                     DLA"
     gene            complement(3135..4019)
                     /locus_tag="VNT12_01430"
     CDS             complement(3135..4019)
                     /locus_tag="VNT12_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI44801.1"
                     /translation="MAGRKDRAATLVEASLLLAVLFLGTNPVAVKVAVAEFPPLPFVT
                     MRFTLAGLLLLTLVALLEPADGRPGRRDLLSLAGVGLVGVGANNVAFTLGVSMTTASE
                     TALIYAAVPIWGILLGLALGLERPTPWGILGVCLAFLGVAVVVYGGLTGSTSLLGNLL
                     VVVATVCWGSYAVLSLPLLRRYSPLVVASYTMLFGGLGALPLALPGFLDAGWAGVSGR
                     AWEALAYSMLLVAAFGFWAWQRGVSQIGANRVLIYQYLITLVGVAAGVLLLGESLTAN
                     KVLGGAVILLGVYLARRR"
     gene            complement(4138..>5347)
                     /locus_tag="VNT12_01435"
     CDS             complement(4138..>5347)
                     /locus_tag="VNT12_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI44802.1"
                     /translation="VLFTDLDEFKIINDSLGHEAGDALLTLVAQRLKRCLRPEDSLAR
                     FGGDEFVVLIEDIENPEVAVRVAERITEELKRPFFLEGRELFAPSSIGIGLGDARTKT
                     PEALLMDADTAMYRAKEEGGTFRVFDPDMYERAKGRLELEADLRRTLDAPYERLPVFY
                     QPMVSILNGAIVGMEALLRWAHPEQGLLVPPGFIPMAEETGLIVPMGRWVLNEACRHA
                     KDWQERYPKNAQLTMAVNISARQLRYPELVGEVEDALRKASLDPGSLTLEITESVLVE
                     DEGSSAGMLQRLKELGVRLAIDDFGVGYSSLSYLRYLPVDQLKLDRVLVGNLDTDDKN
                     LAIVRAAVDLGHALGIEVVAEGVETQEEFEKLSKLGCDVGQGDYWWGPHLPRETEELL
                     ASNYTSSPDQ"
BASE COUNT          939 a         1853 c         1569 g          986 t
ORIGIN      
        1 cttcttccaa cctcccggcg acgacgttag ccacggccgc gctaatggcc tcatagtcag
       61 ggaagacgcg cgccctcaca gctctgagac gaccttcttg gcccttggta tctggggggc
      121 ggccatgctc aactccgtaa cgccaagctc gacgagcttg gggatcatct ccggctcgcc
      181 ggccgcctcg ccgcatacac ctactggaat gccagccgcg cctgcggcca cgcaggtctg
      241 ccggatcagg gccagaaccg cgggatggtc agcgctctgg agccgtcgca gccgctcgtt
      301 gcccctgtcc gcggccaggg tgtactggac gagatcattc gtccctatag agaagaaggc
      361 tgactcctcg gcgagtttcg cagcgcagac cgcggccgct ggcgtctcga tcatcactcc
      421 cgtctcgacc tcgccgatct ccactccttc ccattcgagc tcccttcggc actcctgtag
      481 tgtctccttc gcggcctcta actctccaac gtcagcgacc atcgggaaca tgaccttcag
      541 gttgccgttg gctgcggcgc gcaagatggc tcgcagctgc ggctcgaaga gctcgggtgt
      601 atccaggctc atccttattc cgcgccagcc gaggaaaggg ttctcttcgg cggcctggtc
      661 cacgccgggc aggtctttgt cgcccccgac gtcgagcgtg cgaacgatga cgggcttctc
      721 gccgaatgcg cgggcaacgg cgctgtacgc ctcgtactgt tcatcctcgg aaggcaggtc
      781 gtcccgctcc atgaacagga actcggtacg gaagagcccg acgccttcgg cgccccactg
      841 gagcgcgccc tcagcctcag aggccgagcc gaggtttgcc gcgacctcga tgcgcctccc
      901 ttcgctggtg ctcgcctcga tgtgacggta cacgtcgagg gccgcccgct cctcggccag
      961 cgccccagac atcctctcga actcagcgac cgtctccgcg tcggggtccg cgatcgcgta
     1021 gccctctccc ccgtcgaccg cgacggtctc cgcgcccaag attctctcca acgcgggtcc
     1081 gacgccgacg acggcgggga tgcccatgga gcgggccatg atcgagacgt gcgaggtcct
     1141 cgacccctcg gcggtgacaa acccgagcgc catccctttc tggatgcgcg ctgtgtccga
     1201 tggtgtgagg ctatgggaga gcacgacgga gggcgtcttt agtgcctcga aggccgagag
     1261 gccacggccc attagctggg cagagatctg gcccgtaacg tcgcgcacgt cgtcggccct
     1321 tgcggccatg tactcgtcat ccatcgcagc gtactccgca gcgtactcct caccgacggc
     1381 aagcaccgcc gcctcggggc tctccaggtt cactacacgc tcctcgaccg ctgaggcaag
     1441 ctcggggtcc tcggccatct cgacgtggaa gtcgaagata ccggcttcct cttcgctccc
     1501 cgcagtacgg agcctgtcac cggtctccga gaggttggcg gcgacagttt cggcagccgc
     1561 tttgaacctt tgcagctcgg cttctacctc ctcctctgtt atccggtccc gctcgggctc
     1621 gtgcttgcct gtggcgggca cgaacgccgg tccaacggcc accccttctg acgctcctat
     1681 gccggtcagc ttcaccagga tctagtgttc gtccgccgct atgagctcgg ccaaggtctc
     1741 cacggcctgt tcggcgtcct cgccctcagc acggatggtg accttctcgc cctttctggc
     1801 gagcccggtt atcttcagaa cgctcttcgc atttacttcc cgatcccctt tcacgataac
     1861 gacctgcgag gaaaaccctt tcgccgcctt tacgaaccgc gccgcaggcc ggccgtggat
     1921 accttcctca ggccctaccg tcgcctctct ctgcgccatg aaaaactcct ctcacgcact
     1981 ctctacaagg cccgtatctc cgcccaatga tactctcact cgctctcgaa agcggctatg
     2041 gcgccggccg ccgctgaagc catgagccgc acgtccgaag agatggtgac gaactggaat
     2101 cccttccctg ccatgcgcga ggcgtactcg ggagaacctg tgaagacacc ggcggcgaga
     2161 ccgtgctcgc gtgctgcggc cagcaccctg tcgatagcct cgaccacctc aggctcactg
     2221 cggtccgtgc caggtccatg cccgaggctc tggcccagat ccgagggtcc cacgaagacg
     2281 gcgtccaggc ctgggacgga aagaatctgt tccaggtttt cgagcgcttc gtgggtttcg
     2341 atcatggcca tcgttaccat ggtctcgttc gcgtagtcgg tgtaatcctg gccgccgtag
     2401 agggttgccc tgtaaggacc gtagctgcgg tagccctcag gagggtagcg gcaggccccg
     2461 acgaaggctt cggcttcctc gcgggtgttg atcatcgggc agatcactgc gtagcaccca
     2521 gcgtcgagca cacgcatgat gaggcctgga tcgttccacg gcacccgaac gacgggcacg
     2581 gtgtccgtcg tcgagatggc ctggagcata gggacgaggc tcccataatc cacgggcccg
     2641 tgctgcatat ctatggtcag gctgtcccat cccgagtgcg ccatgacctc ggctgagaaa
     2701 gagctaggta cctgcaacca cccgttcaca gcggtctctc cctcacgcca gatcttcttc
     2761 agacgattct ccctcatcct tggatctcct cctccagcgc cacaccgatg catcctgcaa
     2821 cccggtggac gtggccgcca gtccttccac gtcgcaggtg atgccggtcc gttgtccgac
     2881 accctgtcga gtctgagagg ataccagcgc atcggcagtg ttttcatcgg cggcttcccc
     2941 ggcccagcct ttgataccgg cgagcagttc agcgaaggtg agcgcctcgt cccagaccca
     3001 cccgctgtcc tcccagctcc agcaggcggc aaggacctcc gcgacagccg ctatccgcac
     3061 acctacgcgc agtccaactc aagctccgct gcgagctggt ctatcgtaga ggagggtatg
     3121 gcccgccagg atgcctaccg acgccgggcg aggtagacgc ccaggaggat gaccgcgccg
     3181 cccaggacct tgttagccgt gaggctctcc ccgagcagca acacgcccgc tgctacgccg
     3241 acgagcgtga tcaggtactg gtagatgagc actcggttcg ctccgatctg tgagacgccc
     3301 ctctgccagg cccagaaccc gaaggccgcc accaggagca tggagtaggc cagggcctcc
     3361 caggcccttc cgctgacccc ggcccagccg gcgtccaaga agcctggcag ggcgagaggc
     3421 agggctccca ggcccccgaa cagcatcgtg taagacgcca ccaccagcgg cgagtacctc
     3481 cgcaagagcg gcagagacag gacggcgtag gacccccagc aaaccgtcgc caccacgacc
     3541 aggaggttcc cgaggaggct cgtgctgcca gttagtccac cgtagaccac gactgcaaca
     3601 cccaagaagg cgagacagac gcccaggata ccccagggtg tcggcctctc cagcccgagc
     3661 gcaaggccga ggaggatgcc ccagatcggc accgccgcgt agatcaaggc ggtctcggac
     3721 gctgtggtca tgctcactcc aagcgtaaag gcgacgttgt tcgcccccac tccgacgagg
     3781 cctactcccg ccaaagagag gaggtctcgc cgaccaggtc ttccgtccgc cggctcgagg
     3841 agcgctacga gcgtgaggag caggagcccg gccagggtga acctcatcgt tacgaaggga
     3901 aggggaggga actcggcgac ggcgactttg acggccacgg ggttggtgcc gaggaagaga
     3961 accgcgagga gcagcgaggc ctccacaagc gtcgccgccc tgtccttgcg cccggccatg
     4021 accccaagtc taccgtcttg gcacagcggt caccggctcg ttgggctccc cttcttgcgc
     4081 tctaggctta ccgtctggag caagatatct ctcccgaggc gggacgcgtt atcggggtta
     4141 ttggtcgggc gaggaggtgt agttggatgc aagaagctct tctgtctctc ttgggaggtg
     4201 cggaccccac cagtagtcgc cctgtccgac gtcgcatccc aacttgctaa gcttctcgaa
     4261 ctcctcttgg gtctctacgc cctcggcgac cacctcgatg cccaacgcgt gcccgagatc
     4321 gaccgccgcc cgcacgatag ccaggttctt gtcgtctgtg tccagatttc ccacaagcac
     4381 ccggtccagc ttcagctggt ccacaggcaa gtacctcagg tacgatagcg aagagtaccc
     4441 caccccgaag tcgtctatgg ccagccgcac cccaagttcc ttgaggcgct ggagcatgcc
     4501 tgcgctagat ccttcatcct cgaccaggac gctctctgtg atctccagcg taaggctccc
     4561 ggggtcgagg gaagccttcc tcagggcgtc ctccacttcg ccaacgagtt ctgggtaacg
     4621 cagctgacga gcggagatat taacggccat ggtcagctgg gcgttcttgg gatagcgttc
     4681 ctgccagtcc ttggcgtgcc ggcaagcctc gttgagcacc caccggccca tcgggactat
     4741 caggcccgtc tcttcagcca tgggaatgaa tcccggaggg acgagcaaac cctgctccgg
     4801 atgggcccac ctaagcagcg cctccatccc tacgatcgcg ccgttgagta tagagaccat
     4861 cggctggtag aagacgggta agcgctcata aggagcatca agagtccgtc tgaggtcagc
     4921 ttccagttcc aggcgaccct tggcccgctc atacatatcc gggtcgaaca ccctgaaagt
     4981 cccgccctcc tccttggccc tgtacatcgc cgtgtcggcg tccatgagaa gggcctcggg
     5041 cgtcttcgtc ctggcgtcgc ccaggccgat gcctatgctg gaaggggcga acagctcacg
     5101 gccctccaaa aagaacggtc tctttagttc ctcagtgatc ctctctgcta cacgcactgc
     5161 tacctcaggg ttctcgatgt cctcgatgag caccacgaac tcgtcgcctc cgaagcgggc
     5221 caggctatcc tccgggcgca ggcaacgctt gaggcgctgg gcgacgagcg tgagtagcgc
     5281 atccccggcc tcgtggccga gtgagtcgtt tattatcttg aactcgtcca ggtccgtgaa
     5341 gagtacc
//
LOCUS       DATMJA010000060         2562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_67897, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000060 DATMJA010000000
VERSION     DATMJA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67897"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VNT12_01440"
     CDS             <1..211
                     /locus_tag="VNT12_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44803.1"
                     /translation="LCRYRADYLMVYADSPLDERLKTLPGFSPVDDAPREKYSLYNVD
                     LEKLGNPARGSNRPRETAPPAPEPS"
     gene            294..1706
                     /locus_tag="VNT12_01445"
     CDS             294..1706
                     /locus_tag="VNT12_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HWI44804.1"
                     /translation="MSPLPDPRLSAAILAARSARFASRTLKTGGGSTVPGVVARRVDP
                     KVLTKLSQRLRLGSAAITGTNGKTTTTRMVSKILQTAGVRAVNNSTGANLVTGVTAAL
                     VSDSGLAGTPRSEMGLFEVDEASVPKVAAEAEIEILAILNLFRDQLDRYGELAYTGKV
                     IASSFGDLPQGGAVVLNADDPLVASLGRSARRQVFYGVDEPSLDTGRLQHVADSKDCP
                     VCGTALVYEIVYMGHVGLYDCRRCDFSRPGPAYRASRVRLEGAKGSEFQLSTPKGEAD
                     VRIGLPGLYNVYNALAAAAVAGEAGVGLAEISLGLGEFGGAFGRVERVKAGDRVAFLL
                     LIKNPVGFNEILRTFVTGAEARNVMIAINDNDADGRDVSWLWDVDFEMLADAREEGKT
                     DAAPFTVSGIRAEDMAVRLKYAELPVGPVIPDREEAIRAALQATPPGETLYVLPTYTA
                     MLEIRQTLSELGYTHPFWED"
     gene            1941..>2562
                     /locus_tag="VNT12_01450"
     CDS             1941..>2562
                     /locus_tag="VNT12_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HWI44805.1"
                     /translation="MLTIHHLYADMMNLYGDRGNVIAIKKRCEWRGIPVEVVDVGLGE
                     RIRPTGCDLFLFGGGQDREQALLAEDLSGSKGADLRTVVDEGGVVLGVCGGYQLMGHS
                     YETPEGEKLPGVGIFDLHTEPRKADEGRLIGNVLVRVRTPETGETREVVGFENHGGRT
                     YLGAGLKPLGEVVVGYGNNGRDGTEGARRLNAYGTYLHGSLLPKNPW"
BASE COUNT          499 a          782 c          850 g          431 t
ORIGIN      
        1 attgtgccgc taccgtgcag attacctgat ggtctacgct gacagcccgc tcgacgagcg
       61 gctgaagacc ctgcccggct tctccccagt agacgatgcc cccagagaga aatacagcct
      121 ctacaatgta gacctcgaga agctaggcaa cccggcccgg ggttccaacc ggcctcgcga
      181 gaccgctccg cctgcgcccg agccatcctg aggagccagg acgccggtag gagtggagag
      241 gtgactgaga gatccttccg catgacccca cccagaacca tataatccac gccatgagcc
      301 ccttaccaga cccacgtctc tccgccgcga tactggccgc caggtcggcc cggttcgcga
      361 gcaggacgct gaagaccggc ggaggttcga cggtgccagg ggtagtggcg cgcagggtgg
      421 acccgaaggt gttgaccaag ctctcgcagc ggctgcggct cggttcagcg gcgattacgg
      481 gcaccaacgg caaaacgact acgaccagga tggtctccaa aatactccag accgccggtg
      541 tccgtgccgt gaacaactcg acgggggcga acctggttac cggcgtgacc gcggccctcg
      601 tctcggattc ggggctggcc gggacgcctc gctcggagat ggggctcttc gaggtggatg
      661 aggccagcgt gccaaaggtg gctgcggagg cggagatcga gatcctcgca atcctgaacc
      721 tgttcaggga tcagctcgac cgctacgggg agcttgccta cacgggcaag gtcatagcct
      781 cgtccttcgg ggacctgccg cagggcggcg cggtggtcct gaacgcggac gaccctctcg
      841 tcgcgagcct cgggcgttct gccaggagac aagttttcta cggtgtggac gaaccttctc
      901 tggacaccgg gcgtctgcag cacgtcgctg actccaaaga ctgccccgtg tgcggaacgg
      961 ccctcgtcta cgagatcgtg tacatggggc acgtcggcct ctacgactgc cggcgctgtg
     1021 acttctcgcg gccggggcca gcataccgcg cgagcagggt gcggctcgaa ggagctaaag
     1081 gctcagagtt ccagctctcg accccgaagg gcgaggctga tgtgagaata gggctccccg
     1141 gtctctacaa cgtctacaac gccctcgctg ctgccgcggt ggccggggag gcaggggtcg
     1201 ggctcgcgga gatctccctt ggtctcgggg agttcggggg agccttcggg agggtcgagc
     1261 gggtaaaggc gggggacaga gtggcgtttc tgttgctcat aaagaaccct gtcgggttca
     1321 atgagatcct gcgcaccttc gtcaccggcg cagaagcccg caacgtcatg atcgccataa
     1381 acgacaacga cgccgacggc cgcgatgtct cgtggctctg ggacgtggac ttcgagatgc
     1441 tcgccgacgc gagggaggaa ggcaaaacgg atgccgcccc tttcaccgtc agcggcataa
     1501 gggccgaaga catggccgtg cggctcaagt acgcggagct tcccgttggc cccgtgatcc
     1561 ccgaccgcga ggaggccatt agggccgcac tccaggcgac cccgccaggc gagacgctct
     1621 acgtgctgcc tacgtacacg gctatgttgg agatccggca gaccctgagc gaactgggct
     1681 acactcatcc gttctgggaa gattaggatt tcagcatttc agcacgccgc caggcttcgc
     1741 caggcggttt gtcagccagt cagttatgtg tcgtgtggcg agacgctcgt gcgcttgaga
     1801 gaatgccaga ggaagacatg cggcttcgaa gggagggttc aaaccaggac cacgcagctg
     1861 aaatgctgaa gtgctgacaa gctgccttca tgcagcgtgc tgaaagccgc cgcaggcggc
     1921 gtgcttacaa tgggaggcac atgctaacca tccaccacct ttacgccgac atgatgaacc
     1981 tctacggcga caggggaaat gttatagcca tcaagaagcg ctgcgagtgg cgcggcattc
     2041 ccgtggaggt cgtcgacgtt gggctcggag agaggataag gccgactggc tgcgatctct
     2101 tcctcttcgg cggcggccag gacagggaac aggcgctgct tgcagaagac ctctctggct
     2161 ccaagggcgc ggacctgagg actgtcgtgg acgaaggtgg cgtggtcctc ggggtgtgcg
     2221 gcggctacca gctgatgggc cactcttatg aaacgcccga gggtgagaaa ctccccggag
     2281 tcggcatctt cgacctgcac acggagccca ggaaggctga cgaggggcgc ctgatcggga
     2341 acgtgttggt ccgggtacgg acacccgaaa ccggcgagac gcgcgaggtc gtcggcttcg
     2401 agaaccacgg cggtcgcacc tacctcggcg cgggtctgaa acctctcggc gaagtcgttg
     2461 tgggttacgg caacaacggc agagacggca ccgaaggtgc gcgcaggttg aacgcctacg
     2521 gtacctacct gcacggctcc ttactcccca agaacccctg gc
//
LOCUS       DATMJA010000061         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_423368, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000061 DATMJA010000000
VERSION     DATMJA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423368"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNT12_01455"
     CDS             complement(<1..469)
                     /locus_tag="VNT12_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44806.1"
                     /translation="MTHATRTFDKMGETTQTVVRTSQESARIFTDYTVKAQELNTQFA
                     RRAVETWIDGLRKQTELSQDVAQELFGRAEEQADAYRRFLGQWGNFPMGGFPFPGKAN
                     SVMFPFQRQGMHLVDTATRSAEETLAPATFPIAGYDEKNVDEISTRLDGLSAEQ"
     gene            complement(586..2424)
                     /locus_tag="VNT12_01460"
     CDS             complement(586..2424)
                     /locus_tag="VNT12_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI44807.1"
                     /translation="MSLDLRLDALLTIPALFGPRVSPDGKWVAWSWSRLGPAADVFAA
                     PTDGSQAPLRLTETIEGDTLVVSWTSDSAAILVSQDNDGDERVRLFRVRVADPGVMDP
                     LTAADPNHYLRGGQLHKDGRWLIYAANLDAASGEEIETDRLYKHDLQTDERLVLARPE
                     KGSILWPELNLQGIHILYSRNELHPAGQQVWLVDIEGLEDREILNFGPRIKISASWFP
                     DGRRVLFVAEADSYRRLGMWSMDEESVRWLVEDPTRNIEYSFVPPNGDPVVVIEIEQA
                     GVRASLLDMQIATETSLQHTRGNLVPLAQTENGGWICMYYDALHPVDLVRYDAEDGGS
                     ASFTGLPGRTSIGAERLVAAEDFRWQSVDGIEIQGWLYRTPGEKHGTIVLVHGGPTSH
                     AEDRFNAEIQYLASRGFDVLAPNYRGSTGFGLPFQNSIKEDGWGGREQEDIRCGIEAL
                     VDANVAEPGRVGVTGTSYGGYSAWWAITHFDPEIVAAAAPICGMTDLALDYYATRPDL
                     RPYSEEMMGGSPEDVPDRYRERSPINSVKNIKGELLIVQGLKDPNVTQDNVEAVTKAL
                     QRENIPYELITFEDEGHGIARPKNLRVLYSRLADFFYKAFKVLPDR"
     gene            complement(2431..>2653)
                     /locus_tag="VNT12_01465"
     CDS             complement(2431..>2653)
                     /locus_tag="VNT12_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI44808.1"
                     /translation="RVPSGAGSEGFYSHNGEEFIYVISGRLFVELKDHGTFQVAPGDT
                     LYFPSTTPHRWWADEEPVEAVYVNTPPTF"
BASE COUNT          454 a          806 c          828 g          565 t
ORIGIN      
        1 tctgctcggc gctcaagcca tcgagccgcg tggagatctc atccacgttc ttctcgtcgt
       61 agccggcgat ggggaaagtt gccggagcca gggtctcctc cgcgctcctt gtagccgtat
      121 caacgaggtg catcccctgc ctctggaagg ggaacattac gctgttggcc ttgccaggga
      181 acgggaagcc tcccatgggg aagttgcccc actgccctaa gaaacgcctg taagcgtctg
      241 cctgctcctc agccctcccg aagagctcct gcgccacgtc ctgggaaagc tcggtctgct
      301 tgcgcagccc gtctatccag gtctccacgg cgcgccgggc gaactgcgtg ttcaactcct
      361 gcgccttcac agtgtagtcc gtgaagatcc tggccgactc ctgagaagtc ctgaccacgg
      421 tctgcgtggt ctcgcccatc ttgtcgaacg ttctcgtagc gtgcgtcaca acgatacctc
      481 catgtcgtaa tcaccgtaca tccttccgca cttatgtgta ccacgcacat atggagttta
      541 aacatccgtg tgttttggct tccgatcgct ccgctaggtt tgggattatc tgtcaggtaa
      601 gactttgaat gccttataga agaagtcggc gaggcgcgag tagaggacgc gcaggttctt
      661 cgggcgggcg atgccgtgac cctcgtcttc gaaggtgatg agttcgtagg ggatgttttc
      721 gcgttgcaag gctttcgtaa cggcttcgac gttgtcctgg gttacgttcg ggtctttgag
      781 gccctggacg atcaggagtt cccccttgat gttctttacg gagttgatcg gggacctttc
      841 gcggtaacgg tcgggaacat cctcggggga accgcccatc atctcctcgc tgtagggcct
      901 gaggtcggga cgcgtggcgt agtaatcgag tgcgaggtcg gtcataccgc agattggggc
      961 tgcggcggcg acgatctcgg ggtcgaagtg cgtgatcgcc caccacgccg agtatccgcc
     1021 gtaggaggtg ccggtaacgc caacgcgtcc aggctctgcc acgttggcat cgaccagcgc
     1081 ttcgatcccg caccttatgt cttcctgctc ccgaccgccc cagccgtcct cctttatgga
     1141 gttctggaaa gggaggccga aacccgtgct gcccctgtag ttcggcgcca gcacgtcgaa
     1201 gccccgcgag gccaggtact ggatctcggc gttgaaccgg tcttcggcat ggcttgtcgg
     1261 gccgccgtgg accaggacga tggttccatg cttctcgccg ggtgtacggt agagccaacc
     1321 ctgtatctct atgccgtcga cagattgcca gcggaagtct tcggcggcga cgagccgctc
     1381 cgcacctatg ctagttcttc ccggcagacc cgtaaaactg gctgaaccgc cgtcctccgc
     1441 gtcgtaacgt acgaggtcca cggggtgaag ggcatcgtag tacatgcaga tccagccgcc
     1501 attttcggtt tgggccagcg gcacgaggtt gcctctggtg tgctgtagtg aggtttcggt
     1561 tgcgatctgc atgtcgagca gcgaggccct caccccggct tgctcgatct ctattacgac
     1621 taccgggtcc ccgtttggcg gtacgaagga gtactcgatg ttgcgggttg gatcttctac
     1681 gagccagcgc acagactcct catccatgct ccacatgccc agcctgcggt aggagtcggc
     1741 ctcggcgacg aagaggactc tgcgcccgtc ggggaaccag gatgccgaga tcttgatccg
     1801 cgggccgaag ttcaggatct cgcggtcttc gaggccctca atgtccacca gccagacttg
     1861 ctgcccggcc gggtgcaatt cgttacgcga gtagagtatg tgaataccct gaaggttcag
     1921 ctcgggccac aggatactac ccttctcggg tctggctagc accagccgtt cgtccgtttg
     1981 caaatcgtgc ttatagagcc ggtcggtctc gatctcctct ccgctcgcgg cgtcgaggtt
     2041 ggcagcgtag atgagccagc ggccgtcttt atgcaactgg ccgcctctga gatagtggtt
     2101 cgggtctgcc gcagtgagtg gatccatcac tcctgggtca gcgaccctga cccggaagag
     2161 ccggacgcgc tcgtccccgt cgttgtcttg cgatacgagg atcgcagcgc tgtccgaggt
     2221 ccaggagact accaatgtgt ccccctctat ggtctcagtg aggcggagag gagcctgcga
     2281 gccgtctgtg ggtgcggcga acacgtcggc ggcaggcccg aggcgcgacc agctccacgc
     2341 cacccacttc ccgtcgggtg agacgcgcgg cccgaagagc gcagggatgg tgagcagggc
     2401 gtcgagcctg aggtctaggg acacgactgg ctagaacgtc ggcggggtgt tgacgtagac
     2461 ggcctcgacg ggctcctcgt cggcccacca gcgatgcggg gtggtcgagg ggaagtagag
     2521 cgtgtcgcca ggcgcgacct ggaacgtccc atgatccttt agttccacga aaagccggcc
     2581 ggagatcacg tagatgaact cctccccgtt gtgggagtag aaaccctcgc tccccgcgcc
     2641 ggaaggtacc ctt
//
LOCUS       DATMJA010000062         2728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_601313, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000062 DATMJA010000000
VERSION     DATMJA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601313"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /gene="fdhA"
                     /locus_tag="VNT12_01470"
     CDS             <1..1163
                     /gene="fdhA"
                     /locus_tag="VNT12_01470"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR02819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HWI44809.1"
                     /translation="PKLEVPEEVAEAMGMTKEAPHAVILKIVSTNICGSDQHMVRGRT
                     TAPVGQTLGHEITGEVIEKGNDVQFIDIGDLCSVPFNIACGRCRNCKERKTGVCLNVN
                     PARAGSAYGYVDMGGWVGGQAMYVMVPFADFNLLKFPDKEQAMEKILDLTMLSDIFPT
                     GYHGCYTAGVTQGSTVYVAGAGPVGLAAAHSAQILGASVVIVGDLKDERLAQARHFGC
                     ETVNVSEDAELHDQIAEIVGEPEVDCAVDAVGFEASAHGPDAIEAPATVLNAVMSITR
                     AGGAVGVPGLYVTGDPGAADPKAQEGTLEIRIGLGWAKSHALYTGQCPVMKYNRELMM
                     AILHDRAQIARAVNATVISLDQAPQGYKDFDKGTAKKFVLDPHGELQGAERA"
     gene            1280..1921
                     /locus_tag="VNT12_01475"
     CDS             1280..1921
                     /locus_tag="VNT12_01475"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWI44810.1"
                     /translation="METIKSPAEHRVILHNTSWETYERLMKERGESRVPRFAYDRGEL
                     EIMSPSTEHESIAYYVGLLVAVFAEEAGVDLYGAGSTTFDREDLERGFEPDACFYVRN
                     AERVRGKPRMDLRLDPPPDLVIEVDITSPSLDKFSIYARASIPEIWRHDGARLAIFEL
                     RGKEYVEVAESRTLPPLTSEALSRFIEESSSLDIVTWMRRVREWSRDNTGSSS"
     gene            complement(1946..2176)
                     /locus_tag="VNT12_01480"
     CDS             complement(1946..2176)
                     /locus_tag="VNT12_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44811.1"
                     /translation="MRLMGLPNVARYPEATVVRDETSILILFCGPHGEQRMNVPLEYV
                     GGDAEAAELRLLAQLQQIGYTVRRGEREPSDL"
     gene            2267..>2728
                     /locus_tag="VNT12_01485"
     CDS             2267..>2728
                     /locus_tag="VNT12_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWI44812.1"
                     /translation="MLEKTSGRVRRGSKTKTRVRVVEDGRVRVRPDALATEEPMEIRL
                     LAGQTKQTVAVTMRTPGADFELAAGFLYGEGIVDSPDDVQKISYCVDSDLDAEQRYNI
                     VNVELRGGHEFDLRPLERHFYTTSACGVCGKASLEQLELRGCPVMTAGPEVS"
BASE COUNT          540 a          836 c          894 g          458 t
ORIGIN      
        1 acccgaagct agaggtaccc gaagaggtcg ccgaggccat ggggatgacc aaagaggccc
       61 cgcacgccgt catactcaag atcgtctcta ccaacatttg tggctcggac cagcacatgg
      121 tgcgcggaag gacgacggcg cctgtcgggc agacgctggg gcacgagatc acgggcgagg
      181 tcatcgagaa gggtaacgac gtccagttca tagacatcgg cgacctctgc tcggtaccct
      241 tcaacatcgc ctgtggccgc tgtaggaact gcaaggaacg caagaccggc gtctgcctca
      301 acgtcaaccc cgcgcgagcc ggctcggcct acggctacgt ggacatgggc ggctgggtcg
      361 gcgggcaagc catgtacgtg atggttccct tcgcggactt caacctcctg aaattcccgg
      421 acaaagagca ggcgatggag aagatcctgg acctcaccat gctctcggat atcttcccca
      481 ccggctacca cggctgctac acggctggcg tgacccaggg atcgacggtc tatgtcgcgg
      541 gtgcaggccc ggtcgggctc gcggcggccc actcggccca gatcctcggg gcgtcggtcg
      601 tgatcgtggg cgacctgaag gacgagcgtc tggcgcaggc gcgtcacttc ggctgcgaga
      661 ccgtaaacgt ctcagaggac gcggagctgc acgaccagat cgccgagatc gtgggcgaac
      721 ccgaggtcga ctgcgctgtt gacgccgtag gcttcgaggc gagcgcgcac ggacctgacg
      781 caatcgaggc tccggcgacg gtcctcaacg ccgtcatgag catcacgcgc gctggcggcg
      841 ctgtgggcgt ccctggcctg tatgtcaccg gcgaccctgg cgccgcggac cccaaggccc
      901 aggaggggac gctcgagatc cgcatcggac tcggctgggc caagagccac gcgctctaca
      961 ccggccagtg cccggtcatg aagtacaacc gggagctgat gatggcgatc ctccacgaca
     1021 gggcccagat cgccagggcc gtcaacgcca cggtcatctc gctcgatcag gctccgcaag
     1081 gctacaagga tttcgacaag ggtacggcca agaagtttgt gctcgaccca cacggcgagc
     1141 ttcagggcgc cgagagagcc tgagagcgat cgcacgagag gggcggacct aagggggccg
     1201 ggattacccg gtccccttta tatctgcgtt ctgtggtgcg acgctataat tcgtcaagag
     1261 cgattcagag aggtcgtaga tggagacgat caagagtccg gccgaacaca gggtcatcct
     1321 gcacaacacg agttgggaga cctacgaacg cctgatgaag gagcgcgggg agagccgtgt
     1381 gccgcggttc gcctacgacc ggggggaact ggagatcatg agcccttcca cggagcacga
     1441 gagcatcgcc tactatgtcg ggctgctcgt ggcagtgttc gcggaggagg cgggagtaga
     1501 tctttacggt gcggggtcca cgaccttcga ccgcgaggat cttgaacgcg gcttcgagcc
     1561 cgatgcctgt ttctacgttc gaaacgcaga gcgtgttcgt ggcaagccca ggatggatct
     1621 gaggctggat ccaccacctg acctggtcat cgaggtcgac atcacgagcc cctctctcga
     1681 caaattctcc atctacgccc gagcaagcat ccccgagatc tggcgccacg acggcgcaag
     1741 gctggcgatc ttcgagctgc gcggcaaaga gtacgtcgag gtcgcagaga gcaggaccct
     1801 cccccctctc acgagcgaag ccctctccag gttcatcgag gagagctctt cactagatat
     1861 cgtgacctgg atgcgccggg tgcgcgagtg gtcgcgggac aataccgggt cgtctagctg
     1921 aacacgcccc gactttatcc tgcggtcaga gatctgaggg ttctcgctct ccacgcctga
     1981 cggtgtagcc tatctgctgt agctgcgcga gcagccgcag ctccgccgct tccgcgtccc
     2041 cgccgacgta ctcgagcggg acgttcatcc tctgttcccc gtggggacca cagaagagga
     2101 tgagtatgct cgtctcgtcc ctgactacgg tcgcctcggg gtagcgggca acgttaggga
     2161 ggcccatcag tctcaagcta ttcgcccgat ctcatcgtac aacattcatg catgctaaca
     2221 cgctgccgct gatacactct ggggaggacc ggaccaggag aggccgatgc tggagaagac
     2281 ctcgggcaga gtcaggcgcg ggagcaagac gaagacgcgg gtacgagtcg tcgaggacgg
     2341 tcgggtacgc gtgcggcccg acgcgctcgc gaccgaggag ccaatggaga taaggctcct
     2401 cgccgggcag acgaagcaga ccgtcgccgt cacgatgcgc acccctgggg ctgacttcga
     2461 gctcgcggcg gggttcctgt acggcgaagg tatcgttgac tctcccgacg atgttcagaa
     2521 gatcagctac tgcgtagact cggacctcga cgccgagcaa aggtacaaca tcgtcaacgt
     2581 tgagttgcgc ggagggcatg agtttgacct gcggccgctg gagagacact tctacacaac
     2641 gagcgcctgc ggcgtgtgcg gcaaagccag cctggagcag ttggaactgc gtggctgccc
     2701 ggtgatgact gccgggcccg aggtttcg
//
LOCUS       DATMJA010000063         5151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_423545, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000063 DATMJA010000000
VERSION     DATMJA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423545"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VNT12_01490"
     CDS             <1..202
                     /locus_tag="VNT12_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWI44813.1"
                     /translation="RVPYVRVNELVNGKRRVTPSTALRLAKAFGTSPEFWLNGQLALD
                     LYQAMNDEQEINEVEKIEPVLR"
     gene            complement(311..1279)
                     /gene="corA"
                     /locus_tag="VNT12_01495"
     CDS             complement(311..1279)
                     /gene="corA"
                     /locus_tag="VNT12_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWI44814.1"
                     /translation="MIVDNAIYVDGHRTAEPATLEETYEAVRNRRGVAWIGLYKPTKE
                     EFSSVAGEFGLHGLAVEDATDAHQRPKLERYGDTLFVVLKPARYLDEEERVEFGEVHL
                     FVGEDFVVSVRHGEAPDLGGVRKQLEADPEHLRRGPEAILHAIMDRVVDDYGPVVDGL
                     ENDIEEIETEVFGGNPSVSRRIYELSREVIEFQRATEPLAGVLGDMMRDEAIDVDPEV
                     RRHLRDVRDHVLQVTERLAGFRELLQSILSVNLTLASLAQNEQVKKISAWAAILFAPT
                     LIGTIYGMNFDYMPELAWPLGYPFAVMLMAFISLTLYLIFKRRGWM"
     gene            complement(1355..1714)
                     /locus_tag="VNT12_01500"
     CDS             complement(1355..1714)
                     /locus_tag="VNT12_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI44815.1"
                     /translation="MGWNELQTRDSEAAGDFYSGLFGWETEPIEDDGRIVYTTIKNAG
                     NQNGGFMPMAEQHGDAPSFWLPYFTVPSRDAAMEKARGLGGTVLGGPLDLPSGKIAVL
                     GDPQGAAFAIFEGETDE"
     gene            complement(1715..1975)
                     /locus_tag="VNT12_01505"
     CDS             complement(1715..1975)
                     /locus_tag="VNT12_01505"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI44816.1"
                     /translation="MPTGHSEGPPADQQEEQPAHWNNYVSVTSAEETVARAKRLGARI
                     LEEPFDVAGFGRLAVLADPAGTVLCVWEPRSHIGAGRVNDVG"
     gene            complement(1978..2349)
                     /locus_tag="VNT12_01510"
     CDS             complement(1978..2349)
                     /locus_tag="VNT12_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HWI44817.1"
                     /translation="MDRTTHSPRITRLSWGRLETESAVFKDVRLYPGGADEWDWNETG
                     TSHNPGIQPADVERLLERGSTVVILSRGFHERLGVAPETLRMLEEREIPAHVLQTEEA
                     ARLYEELRETERVGALIHSTC"
     gene            complement(2381..3184)
                     /locus_tag="VNT12_01515"
     CDS             complement(2381..3184)
                     /locus_tag="VNT12_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HWI44818.1"
                     /translation="MSESGQQRNESPLLSDRGATTISDTVVSTIAGVAAREVEGVHMG
                     GGASRTAGGVLGSLTGSESKASGISVEVGRTETAIDLKMGIEYNKNILQTVEEVRRRI
                     TDRVQSMTGLRIKELNATITDITFPEKEQRRRIGSRSATMERDASADETTTAPAQATM
                     PVSEPDPDASERETTQVGTRSQAETETPPEEEASVEGRQPEEAETADLPSGDTRTEEQ
                     PAGEAPETSEEAETRAETETSEPTETAAEARRRRRAEERARRRRRTEDS"
     gene            3404..4228
                     /locus_tag="VNT12_01520"
     CDS             3404..4228
                     /locus_tag="VNT12_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI44819.1"
                     /translation="MRRRVILIGLHFVVLLLVGCGGPSGESGPTDKCELDNPPLFEKG
                     VLTVATDKPAYAPWFKGSSKDYSGYEGDIASEIAERMDLPIKWTVEPFNKSYAPGAKD
                     FDFDINEITITPEREKAVDFSDGYFNNAQGVLVMKDSPAADAKSISDLKDLTIGAQVG
                     TTALDFVNETVRPTTNPKVYDTTNDAKSALESGQIDVFVTDLVTTVYLRDVVIEGSTV
                     IGKYPRNEQFGMLFEQGNPLVGCVNEILGEMKNDGTLQKLKQQHLQQYLDVPTLEK"
     gene            4308..5102
                     /locus_tag="VNT12_01525"
     CDS             4308..5102
                     /locus_tag="VNT12_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWI44820.1"
                     /translation="MSSASTVLFFAVIAVVVVLAPGSNVVAERFFSPSHFWQSLVGSE
                     GNPSVLGAFLLNVKIFTVSEIFILILALVIAVVRRIPGPVFFPFRLVAVAYTDLFRGV
                     PLILVLYMIGFGVPGLGLGFVSYLPDVTYGVISLVLVYSAYVAEVYRAGIESVHESQN
                     AAARSLGLTRWQSLRFVVLPQAIRRVIPPLLNDFIGLQKDTALVSVLGSVEAARAAQI
                     YSASEFNYASYVVAALLFVLITIPLARFTDRLIARDKRRREAGALA"
BASE COUNT          866 a         1679 c         1553 g         1053 t
ORIGIN      
        1 cagggtaccc tacgttcggg tcaacgagct agtgaacggc aagcggcgtg tcactccctc
       61 aacggcgttg cggctggcga aggcatttgg cactagtccg gagttctggc tgaacggaca
      121 gctcgcgctc gatctctacc aggcgatgaa cgacgagcaa gagatcaatg aggtagagaa
      181 gatagagcca gttctgcgct agatcctggt cctcccctgc gaccaccgcg gcggcatccc
      241 cctcgatagt aaccgggaga ctgcccacga ggacgttgcg tggttcgtta gtcttcagac
      301 tatcgttggc ctacatccag ccgcggcgct tgaaaatgag gtagagcgtg agggagatga
      361 aagccatgag catcacggcg aaggggtagc cgagcggcca ggccaactcg ggcatgtagt
      421 cgaagttcat cccgtagatc gtccctatga gcgtgggcgc gaacagtatg gcggcccagg
      481 cggagatctt ctttacctgc tcgttctggg ccaggctagc cagggtcagg ttcacgctga
      541 ggatgctctg cagcagctca cggaaaccgg cgagccgttc cgtaacctgc aggacatggt
      601 cacggacgtc gcggagatgc ctccgtacct caggatcgac gtctatggcc tcgtcccgca
      661 tcatatctcc aagcacaccg gcgagcggct ctgtcgccct ctggaactcg atcacttcgc
      721 gcgagagctc gtagatcctg cgcgacacac tgggatttcc cccgaagacc tcggtctcta
      781 tctcctcgat atcgttctcg agcccgtcga ctacgggtcc gtaatcgtct acgacacggt
      841 ccattatggc gtgcaggatc gcctcgggcc cacggcgcag gtgctcgggg tcggcctcca
      901 gttgcttgcg tactcccccc aggtccggcg cctctccgtg acgcacgctc accacaaagt
      961 cttctcctac aaagaggtgg acttcgccga actcgaccct ctcctcctcg tccaggtacc
     1021 gggccggttt gagcacgacg aagagcgtat ccccgtagcg ctccagcttg ggcctctggt
     1081 gggcgtccgt cgcatcctcg accgcaagcc cgtgcaggcc gaactccccg gcgacggacg
     1141 agaactcctc tttcgtcggc ttgtagaggc cgatccacgc caccccgcgc cggtttcgga
     1201 cggcctcata ggtctcttcg agggtggccg gctcggcggt acggtggccg tccacgtaga
     1261 tggcgttgtc cacgatcaca gcagcattat acgcctgtgc ctgttgctcc tggcgcacct
     1321 cagcgcggcg acgcgcctgt actactgcgc gcccttactc gtcggtctcg ccctcgaaga
     1381 tggcgaacgc ggcgccctgg gggtcgccaa ggacagcgat cttgccagag gggagatcca
     1441 aaggcccgcc cagcacggta cctccaagcc cgcgcgcctt ctccatggcg gcgtcgcgcg
     1501 aggggaccgt gaagtaaggg agccagaagg aaggcgcgtc gccgtgctgc tccgccatcg
     1561 gcatgaaccc gccgttctgg ttgccagcgt tcttgatcgt agtgtagacg attcttccat
     1621 cgtcctcgat cggttcggtc tcccaaccga agagcccgct gtagaaatcg cccgccgcct
     1681 cggagtcgcg ggtctggagc tcgttccagc ccattcagcc aacatcgttg acccgtcccg
     1741 cgccgatgtg cgaccgcggc tcccatacgc agagcaccgt gccggcgggg tcggcgagca
     1801 cggccaggcg gccgaacccg gccacgtcga agggctcctc gagtatcctg gcgcccagtc
     1861 gcttcgccct tgcgacggtc tcttcggcgc tcgtgacgga gacatagtta ttccagtgcg
     1921 cgggctgctc ctcctgctgg tcggccggtg gaccttcaga gtggccggtc ggcatggtca
     1981 gcaggtcgag tggataagcg ctcctactct ctcagtctcc cgcaactctt cgtagaggcg
     2041 ggcagcctct tcggtctgta ggacgtgcgc ggggatctcc ctttcttcca gcatcctgag
     2101 ggtctcgggc gcgacgccga gacgttcatg aaagcctcta gagaggatca cgaccgtcga
     2161 gccgcgttcc agcagtctct ccacgtcggc cggctgtatg ccgggattgt ggcttgtacc
     2221 cgtctcgttc cagtcccact cgtccgcgcc tccggggtag agccttacgt ccttgaagac
     2281 ggcgctttcg gtctccaggc ggccccagga gagccgggtt atccgcggag agtgtgttgt
     2341 ccgatccatc ttgctcgccc tccaagagat ccgcggggtt ctagctgtct tcggtacgcc
     2401 tgcgccgcct ggcgcgctcc tccgcgcggc gcctgcgtcg cgcctctgcg gccgtttccg
     2461 tcggctcgga ggtctcggtc tctgcgcgtg tctccgcctc ctcagacgtt tccggcgctt
     2521 cgcccgccgg ctgctcctct gtcctggtgt cgccagaagg caggtctgcg gtctcggctt
     2581 cctcgggttg gcggccttcc acgctggcct cttcctcagg aggagtctcg gtctcggcct
     2641 ggctgcgcgt ccctacttgc gtggtctcgc gctcgctagc gtcgggatcg ggttcgctca
     2701 caggcatcgt tgcctgcgcc ggggcggtag tcgtttcgtc cgcggaggcg tcgcgctcca
     2761 tcgttgcgct ccgcgacccg atgcggcgtc tctgttcctt ctcggggaag gtgatgtccg
     2821 ttatcgtggc gttaagctct ttgatcctga gccccgtcat gctctggacg cggtccgtga
     2881 tcctgcgccg cacctcctcg acggtctgga ggatgttctt gttgtattcg atccccatct
     2941 tgagatctat ggctgtctct gtcctcccga cctcgacgga gataccgctg gccttgctct
     3001 ccgaacccgt gaggctcccc agtacgccgc cggcggtcct cgaggcgccc ccgcccatgt
     3061 ggacgccctc aacctcccgc gccgccacgc cggctatagt ggagactaca gtgtcgctta
     3121 tagtcgtggc cccgcggtca ctcaggagcg ggctttcgtt gcgctgctgc cctgactcgc
     3181 tcatctctca ctctcctcct tcgaccaagt tctctagagt gttccccgaa cggcccctgc
     3241 gcaaccagaa taccagtatg cgcgctgcga agacccctga cggatggcaa ggggtcgatt
     3301 gcaacgttcg gcgggcgatc ctatacttgc tacagtatac gatggtccct gactttcgaa
     3361 ccggcccatc aatgtccgtc cggagagtag atcggagagg cgcgtgagaa ggcgagttat
     3421 cctgatcggg ctccactttg tggttctatt gctcgtcggt tgcggcggac cttcaggaga
     3481 gtcgggtcct acggataagt gcgaattgga caacccgccg cttttcgaga agggtgtgct
     3541 gacggtcgcc acggacaagc ctgcctacgc gccgtggttc aagggaagtt ccaaggatta
     3601 ctcggggtac gagggcgata tagcgagtga gatcgcagag cgcatggacc tcccgataaa
     3661 gtggaccgtc gagcctttca acaagtccta cgcccctggc gccaaagatt tcgacttcga
     3721 tatcaacgag attaccataa cgccagaacg cgaaaaggcg gtcgatttct cggatggcta
     3781 cttcaacaac gcccagggcg tgcttgtgat gaaagattct cccgccgccg atgcgaagag
     3841 catctccgac ctcaaggacc tgaccatagg ggcccaggtc ggcacgaccg ccctcgattt
     3901 cgtcaacgaa acggtaaggc cgaccactaa ccccaaggtc tacgacacca ccaacgacgc
     3961 gaagtccgcg ctggagagcg gccagatcga cgtcttcgtc accgacctgg tgacaacggt
     4021 ctacctgcgc gacgtcgtga tagagggctc gaccgttatc ggaaagtacc cgcgcaacga
     4081 gcagttcggg atgctcttcg agcagggcaa cccgctcgtc ggctgcgtaa acgagatcct
     4141 cggcgagatg aagaacgatg ggaccctaca gaagctaaag caacagcacc tgcaacagta
     4201 cctggacgtc cccaccctcg agaagtagcg tgacaaacgg aggtcccgcc gccccaggac
     4261 caaccgcggg ccggggctcg cgggccctgg ggaacagagg ggtgctcgtg tcgagcgcga
     4321 gcaccgtact tttcttcgcc gtcatcgcgg tagtagtagt gcttgcgccg ggtagcaacg
     4381 tcgtggccga gcggtttttc agcccttcgc acttctggca atcgcttgtc ggctctgaag
     4441 ggaacccctc ggtcctcggg gcattcttgc tcaacgtaaa gatctttacc gtctccgaaa
     4501 tctttatttt gatcctggcc ctcgttatag cagtggtcag gaggatacca gggcctgtgt
     4561 tcttcccttt caggctggtc gcggtggcgt acacggacct cttcaggggg gtaccattga
     4621 tcctggtcct ttacatgatc gggttcggcg tgcccggtct cggcctgggc ttcgtctcct
     4681 acctccccga cgtgacctac ggtgtgatct cgctggtcct cgtctactcg gcctacgtcg
     4741 ccgaggtcta ccgggccggc atcgagtccg tgcacgaaag ccagaacgca gcggcccgtt
     4801 cactgggact cacgcgctgg caatccctga ggttcgtggt cctgccgcag gccataaggc
     4861 gggtgattcc gcctctcctg aacgacttta tagggttgca gaaggacacg gcgctcgtct
     4921 cggtgctcgg ctcggtggag gcggcgcggg cggcccagat ctacagcgcc tccgagttca
     4981 actacgcgag ctacgtggtc gcggccttgc tcttcgtcct gatcactatc cccctcgccc
     5041 gctttacgga ccgccttata gcccgcgata aacgccgccg cgaggcagga gccctggcat
     5101 gaacccggca ctcctcctcg aaaacgtaca caagtcgttc ggggaaaacg a
//
LOCUS       DATMJA010000064         4196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_660562, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000064 DATMJA010000000
VERSION     DATMJA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660562"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /locus_tag="VNT12_01530"
     CDS             complement(<1..2207)
                     /locus_tag="VNT12_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWI44821.1"
                     /translation="MREAGAKGAPVTGSLRVLLVEDSQNDAMLLLRELRRGGYQPHAQ
                     GVCTPEDMQEALRAADAKGEPFQVVISDYYMPRFRAPDSLKLLRELGYDVPFIVVSGK
                     IGEDAAVEIMKAGANDYLTKENMSRLCPAIEREVREAEVRRERERAEKALDLSEDRFR
                     RLVEQIPAVTYVQEPIDSDTPKAITYMSPQYEAMLGYPAETEILDEEHWLRMLHPDDR
                     ERVLAEELRTDETGEPYRIEYRQIARDGRVVWVRDEATLIRDEEGNALYWLGVQYDIT
                     EQKRTEEELRQSEERFKVLSQEVVEGIILSENGRIIDANRSVTEMFGYRLEELIGMDA
                     IELTPPEYREMVRQRISDEDTRHYESRGLKKDGTIFPVEIRPRHLPYSGRRIRVTSVI
                     DLTEREHQEEALRQSEALYRTVVEQAEENIFLVDAKSKRVLDANDALLRSLGYTDDEL
                     KEMTLYDIVAHDQESVDLNIGRIMVEGHRSLGERQYRRKDGSLADVEVNVSAVPYNGE
                     KIMCVVAHDVTERKRAERMLKEIREAERNRLARELHDSTLQDIVYALQEVQVMQVVSG
                     DDLNPALEDTAEALKRSVEGLRGAIFELRLKETLDRSVVSSLENLVDLNRRMARGRYE
                     VVLVADDDFPARLPDKAGQELTRLVQEALTNVRRHAEASHVRVELGLEGGLAYVEVSD
                     DGGGFDPERATTGIGRQSMRQRALELGGEFSVQSAPGKGTKVRFSIPVTRLVKA"
     gene            complement(2200..>4196)
                     /locus_tag="VNT12_01535"
     CDS             complement(2200..>4196)
                     /locus_tag="VNT12_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HWI44822.1"
                     /translation="WQYTGRPVRIVRWKGSWSGERNVIGGGCVRGRVLHVARGLESLI
                     GRYLLILRSGLGRLLRHLRLAAAAYGVLLISLLLAGFAWYYVRHTVEAQDQVRFDETI
                     RATQAAVDRRTDAYLDALFGARGLFLASKAVERGEWESYVEGIETKSRLEGLQALGFA
                     RYVRPDERAAFAREARREGMPEMRPDLDPGGERSAYFPLALVAPSDEANLSMINQDAY
                     TEQAHRVAMDEARDTGSPQATRMVYVLTEPTANLSADLVLSPGFAVYLPVYAKGEPVG
                     SVAARRRALEGFVVGYFRRDGLLDDVFGSGFDPAIDFEVYDGADVASSSLLYDEDGVQ
                     RAGEEGYDPLFSEKSRFGVAGREWSLYFATLPGFKNGAESNLPAFVLASGIGASVLLF
                     GITWMLVRSRILAERASKDLEDANRELEGTNSELEAFSYSVSHDLRAPLRAIDGFSQI
                     LQEDYGHALDEEGMNYLGRVRAASGHMATLIDDLLDLSRVGRRPLRRELVDLARLATG
                     IVEELRASQPEREVEFVAGENIMAWGDASLLKVALENLLGNAWKFTAREEEARIEFGA
                     DRKPGPGLPSPVYYVRDNGAGFDQTYADKLFGAFQRLHGQDEFEGTGIGLATVARIVH
                     RHGGRVWAEGTVGEGATFYFTLGGQDIGDRTVPAKKAEVA"
BASE COUNT          685 a         1453 c         1199 g          859 t
ORIGIN      
        1 gctttgacca ggcgggtgac tggtatggag aagcgcacct tcgtcccctt gcccggcgcg
       61 ctctgtacgc taaactcccc gccgagttcg agagcccgtt gcctcatgga ttgcctgcct
      121 ataccggtgg ttgccctctc tgggtcgaac ccacctccgt cgtcggacac ctcgacgtag
      181 gcgagcccgc cttcgagacc cagctctacc ctgacgtggc tggcctcggc gtgtcgtcgg
      241 acgttcgtga gcgcctcctg gacgaggcgc gtcagctcct ggcctgcctt atccgggagc
      301 ctggctggaa aatcgtcatc tgctaccagt actacttcgt accttccacg ggccatccgc
      361 cggttcagat ccacgaggtt ctcgagcgag gagaccacag agcggtccag cgtctctttg
      421 aggcgcagtt cgaagatggc gccgcgtagc ccctcgacgg agcgcttgag ggcctcggcc
      481 gtgtcctcta gtgccggatt cagatcgtcc cccgagacga cctgcatgac ctgtacctcc
      541 tgcagcgcgt agacgatgtc ctgaagggta ctgtcgtgaa gctccctggc cagcctgttg
      601 cgttcggctt cccggatctc cttgagcatg cgctctgccc gcttgcgctc tgtcacatcg
      661 tgggcgacga cgcacatgat cttttcgccg ttgtagggta cggcactcac gttgacctcg
      721 acatcagcca gcgagccgtc cttgcgacgg tactgcctct caccgaggga acggtgaccc
      781 tccaccataa ttcgtccgat gttgaggtcc acactctcct gatcgtgggc gacgatatcg
      841 tagagcgtca tctctttcag ctcatcgtct gtatagccca acgaacggag gagggcgtcg
      901 ttggcgtcga gcacacgctt actcttcgca tctacgagga agatgttctc ctccgcctgc
      961 tcgaccaccg tgcggtagag cgcctcgctc tgcctcaggg cctcctcctg gtgctcgcgc
     1021 tctgtcaggt ctatcacgga ggtcacccgg atccgacgcc cggaataggg taggtgcctg
     1081 ggccttatct ctacaggaaa aatggtgccg tctttcttga ggcccctcga ctcgtagtgt
     1141 ctggtgtcct cgtcggagat acgctgcctc accatctccc tgtactcagg cggtgtaagc
     1201 tcgatggcgt ccattccgat cagttcctcg agcctgtaac cgaacatctc ggtaacgcta
     1261 cggttggcgt cgatgatcct gccgttctcg ctgaggatta ttccctcgac gacctcctgg
     1321 gagagcacct tgaagcgttc ctcgctctgc ctgagctcct cctcggtccg cttctgctcg
     1381 gtgatgtcgt actggacgcc gagccagtac agagcgttgc cttcctcgtc cctgatgagc
     1441 gtcgcctcgt cgcgtaccca caccacccgc ccgtcgcggg cgatctgacg gtactctatc
     1501 ctgtaaggct caccggtctc gtcggtcctg agctcttcgg caagcaccct ttctctgtcg
     1561 tcgggatgca gcatcctgag ccagtgctcc tcgtcgagta tctctgtctc cgcaggataa
     1621 cccagcatag cctcgtactg cgggctcata tacgttatgg ccttcggagt gtcggagtcg
     1681 ataggttcct gcacgtacgt aacggccggt atctgctcta cgaggcgcct gaagcggtct
     1741 tcgctcaggt ccagcgcctt ctccgccctc tcgcgctccc tcctgacctc cgcctccctg
     1801 acctcgcgct cgatggcggg acacagcctg ctcatgttct ccttggtcag gtagtcgttg
     1861 gcccccgcct tcatgatctc caccgccgcg tcctccccta tcttgcccga gacgacgatg
     1921 aaagggacgt cgtaaccaag ctcccgtagt agcttcaggg agtcgggcgc cctgaagcgt
     1981 ggcatgtaat agtcggagat cacgacctgg aaaggctcgc ctttggcgtc cgccgcacga
     2041 agcgcctcct gcatgtcttc cggggtacag accccctgcg cgtggggctg gtatccgccg
     2101 cgtcttagct cccgcaggag aagcatggcg tcgttctggg agtcctcgac gagcagcacg
     2161 cgcaggctcc ctgtcacagg cgcccctttc gcgccagcct cacgcaactt cggccttctt
     2221 ggcggggacc gtacggtctc cgatatcctg gcctcctagc gtgaagtaga acgtggcccc
     2281 ctcacccacc gttccctccg cccagaccct gccgccgtgc ctgtggacga tgcgagctac
     2341 cgtcgccagg cctatccccg tcccctcgaa ctcatcctgc ccgtgcagcc tctggaaggc
     2401 gccgaacagc ttgtctgcat acgtctggtc gaagccggcc ccgttgtccc gcacgtagta
     2461 aactggggat ggcaaacctg gaccgggttt cctgtcggcg ccgaactcta tgcgcgcctc
     2521 ttcttccctg gcggtgaact tccacgcgtt gccgagcagg ttctcgagcg ccaccttgag
     2581 caggctcgcg tctccccagg ccattatgtt ctctcctgcc acgaactcga cttctcgctc
     2641 tggctgcgac gccctaagtt cctcgactat ccccgtggcg aggcgggcca ggtccacgag
     2701 ttccctgcgc aggggcctgc gtccaaccct cgagagatcg agcaggtcgt ctattagggt
     2761 ggccatgtgc ccgctcgccg cccttactcg accgaggtag ttcataccct cctcgtcgag
     2821 cgcgtgcccg tagtcctcct gcaatatctg tgagaagccg tctatggccc tcaagggagc
     2881 cctcaggtcg tgggagacgg aataagagaa cgcctcgagc tcactgttgg tcccttccag
     2941 ttccctgttg gcgtcctcca ggtccttgct cgcgcgctcg gccaggatgc ggctgcgcac
     3001 gagcatccag gtgatgccga agagcaagac gctggctcct atgccgctgg cgagcacgaa
     3061 ggccggcagg ttgctctcgg caccattctt gaatcctggc aacgtggcga agtacaggct
     3121 ccactcgcgc ccagccaccc cgaagcggct cttctcggag aagagcggat cgtacccctc
     3181 ctctccggcg cgctgcaccc cgtcttcgtc gtagaggagg gagctcgagg caacgtcggc
     3241 accgtcgtac acctcgaagt ctatggccgg gtcgaagccg ctcccgaaca cgtcgtcgag
     3301 cagcccgtcc cgcctgaaat atccgacgac gaacccttcg agggcgcgcc ttcgtgcagc
     3361 gacactcccc acaggctctc ccttggcgta gaccggaagg tacacggcga agccggggct
     3421 gaggacgagg tcggcgctca agttcgcggt tggctccgtg agcacgtaga ccatcctggt
     3481 agcctgcgga gagcctgtgt ctcgcgcctc gtccatggca accctgtggg cctgctcagt
     3541 gtaggcatcc tggtttatca tgctcaggtt ggcctcgtcc gaaggggcga ccagcgcgag
     3601 gggaaagtag gcagaacgct ccccgccggg atccaggtcg ggcctcatct caggcatccc
     3661 ctctctcctg gcctccctgg cgaaagccgc cctctcgtcg ggccttacat atctggcgaa
     3721 gccaagcgcc tgcaagccct ccagccggct cttggtctct atgccctcga cgtagctctc
     3781 ccactctccg cgctcgaccg ccttgctggc caggaacaag ccgcgggcgc cgaacagcgc
     3841 gtcgaggtaa gcgtccgtcc gccggtctac ggccgcctga gtagcccgga tggtctcatc
     3901 gaaacggacc tgatcctgcg cctcgaccgt atggcgcacg tagtaccagg caaacccggc
     3961 gagaaggagc gagatcagga gcaccccgta agccgccgca gcgaggcgca ggtggcgcaa
     4021 gagccggcct agccccgacc gcaaaatgag caggtaccgg cctatgagag actccaaccc
     4081 acgagcaacg tgcagtacac ggccacggac gcatccgcca ccgataacgt tgcgctcacc
     4141 gctccacgaa cctttccatc ggactatccg cacgggacgc cccgtatatt gccatg
//
LOCUS       DATMJA010000065         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_779483, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000065 DATMJA010000000
VERSION     DATMJA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779483"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VNT12_01540"
     CDS             complement(<1..423)
                     /locus_tag="VNT12_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI44823.1"
                     /translation="MEEGAARGRSVSRLNVLIAITAAIIGLIYGYDLGSIASALLFLV
                     PAFDLSTFMTSVVTSAVVLGQLCGALFAGRISNTIGRKRSLVLVALGYAAFAGLQGLA
                     PNEWFLTVVRFLLGLAIGISIVVAPAYIAESAPVSVRGS"
     gene            520..1554
                     /locus_tag="VNT12_01545"
     CDS             520..1554
                     /locus_tag="VNT12_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWI44824.1"
                     /translation="MTWGSVKRLHVYCDPASKGHVNGLAGVESPERFPAALAGVERAE
                     QSGVRVEWRPCSPAERESLVAVHDQAYLDDLREMSAAGEGYLSLDTAVSAGSWEAASF
                     ASGAACSAVESALTGETAFAVARPPGHHAGSDYGMGFCLTNHAAVAAGHALSRGLESV
                     AILDWDVHHGNGTQDIFYGDGRVLYLSMHQSPFYPGTGKTSEVGEEEGRGFTVNVPLP
                     AGSGEDIYAAVFDGVFVPVLREFGPEVILISAGFDAHVDDLLGGMALRSDSFGRFAAL
                     ISDLSREVGAAPPAFVLEGGYNLDALTESVAATMQGVGKRAPDWEDADGVRYVQKSRE
                     TLAPFWRSLR"
     gene            1559..2236
                     /locus_tag="VNT12_01550"
     CDS             1559..2236
                     /locus_tag="VNT12_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HWI44825.1"
                     /translation="MRVFGFPPFEDRRDAGRRLAGKLSRFEDEQTVVLALPRGGVPVG
                     YEISRALEAPLDVFVSRKLGAPGQPEFGIGAVAAGGVRVLNQDVVRRLGIPDDYVQEI
                     TAQELAEVGRRLRYFRGERPETEVVGRTAILVDDGLATGVTARAAVQALRLREPRRLV
                     LAAPVCAAQTADLFRTVVDELVCLESPSDLGAIGFWYRNFEQTSDEEVVELLERARSE
                     RGEEGSP"
BASE COUNT          434 a          707 c          823 g          392 t
ORIGIN      
        1 cgagcccctg acgctgacgg gcgcggactc ggcgatgtat gccggcgcca cgacgatgga
       61 gatcccgatg gcgagaccga ggaggaacct gacgacggtc aggaaccatt cgttgggggc
      121 cagcccctgc agtccggcga acgccgcgta gccgagcgcg accagcacca gagagcgctt
      181 acgcccgata gtgtttgaga tgcgtcccgc aaagagcgca ccgcacaact gcccgagcac
      241 aacggctgag gtaacaacgg aggtcatgaa tgtactgagg tcgaaagcgg ggacgaggaa
      301 gaggagtgcg ctcgcgatag agcccaggtc gtagccgtag atcaggccga tgatcgcggc
      361 cgtgatggca ataaggacgt tgagcctcga tacgcttcgc ccgcgcgctg caccttcttc
      421 cacggcaaac cttctttccc caggggtccc atccgagatg ctggcatagt agcatagtgg
      481 gtgaggacct agggcccgac aaaacgtgga gatggaatcg tgacctgggg gagcgtgaaa
      541 cgtttgcacg tctactgtga cccggcctcg aagggccacg tgaacggcct cgccggtgtg
      601 gaatccccgg agaggttccc tgctgccctc gcaggggtcg aaagggctga gcaaagcggc
      661 gtacgtgtgg agtggcgccc atgtagtccc gcggagagag aatccctcgt cgccgtccac
      721 gaccaggctt acctggacga cctgcgagag atgtccgcgg cgggagaggg ctacctcagc
      781 ctggatactg cggtcagcgc gggttcctgg gaagcggcct ctttcgcgag cggggccgcc
      841 tgctcggcgg tcgagagtgc cctcacaggc gagacggcct ttgccgtcgc ccgtcctcca
      901 ggccatcacg cggggagtga ttacgggatg gggttctgcc tcacgaacca cgcagccgtc
      961 gcagccgggc acgccctttc cagagggctg gaaagcgtcg ccatcctgga ctgggacgtg
     1021 caccacggca acggcacaca ggacatcttc tacggtgatg gacgcgttct ttacctctcg
     1081 atgcatcaga gccccttcta ccctgggacg ggcaagacaa gtgaggtggg ggaagaagaa
     1141 gggcgtggct tcacggtcaa cgtacctctg cctgctggct cgggggagga catctatgcg
     1201 gctgttttcg acggggtttt cgtgcccgtg ctgcgggagt tcgggcccga ggtgatcctg
     1261 atctccgccg gtttcgacgc ccacgtagac gatctactag ggggcatggc gctccggagc
     1321 gactctttcg gacgcttcgc ggccctgatc tcagacctct cccgcgaggt cggggccgca
     1381 ccccctgcgt tcgtcctcga gggaggctac aacctcgacg ccctaaccga gagcgtagcc
     1441 gccaccatgc agggggtcgg taagagagcg ccggactggg aggatgcgga cggggtgagg
     1501 tacgttcaga agagccgcga gaccctggcg ccgttctggc ggagccttcg ctgaggctat
     1561 gagagtcttc ggctttccgc ccttcgagga tcgcagggac gcaggccgcc ggctcgccgg
     1621 gaagctgtcc cgcttcgagg acgagcagac cgtggtcctc gccctaccaa gaggaggggt
     1681 gcctgtgggc tatgagattt cacgcgccct cgaggcgccg ctagacgtgt tcgtctcgcg
     1741 caagctaggg gcgcctgggc agccagagtt cggcatcggg gccgtcgcgg ccggcggcgt
     1801 gcgcgtcctc aaccaggacg tagtcaggcg tctcggcatc cccgacgact acgtgcaaga
     1861 gatcacggcg caagagctcg cggaggtagg gcggaggttg cgctacttca ggggcgagag
     1921 gcccgagacg gaagtggtgg ggcggaccgc gatcctggtc gacgacgggc tcgctaccgg
     1981 ggtgacggcc cgcgccgccg tacaagccct gcgattgcgc gagccgagac gcctggtcct
     2041 ggcagcgcca gtatgcgccg cccagaccgc ggatctcttc aggaccgtgg tggacgagtt
     2101 ggtctgcctg gagtctcctt cggacctcgg ggccatcggc ttctggtaca ggaacttcga
     2161 gcagacctcg gacgaggagg tcgtggaact tctcgaaaga gccaggagcg agcgaggcga
     2221 agaagggagt ccgtagaacc atcctgagcc ccgtcaaccg gccggtggga gacaggcctc
     2281 agaccggtac aattacatta ggtttccgag ttcttgagag gtgagagata tgtgcggcag
     2341 gtatacgctt tccacc
//
LOCUS       DATMJA010000066         3719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_128029, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000066 DATMJA010000000
VERSION     DATMJA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128029"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1187
                     /gene="murG"
                     /locus_tag="VNT12_01555"
     CDS             99..1187
                     /gene="murG"
                     /locus_tag="VNT12_01555"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWI44826.1"
                     /translation="MNEPAKSNHARMPRILIAGGGTGGHAIPALCVAADLKERGAIVE
                     FVGSSSGIEMSLVPQQGYTLHALPLTGLAGSPLAQARAGLLSLRAVIRCTRIIQRLRP
                     LSVLGVGGYASAPAVIAARTLGVPTFLHEQNSVPGRVNRLASRFTTQTLATFPDATRR
                     LGNAAWVGMPTRTEIFEASREEALRDLGLQPPVVLIFGGSGGALRINLAAAEAFGGTT
                     PYTVVQISGRRDFSRLHADNPRHTILEFAGDIWRYLAAADVVVSRAGAGSLFDIAAAG
                     KAAILIPFPYATGDHQLVNARYFTDRGAAELMLDSEVGARELRDRVEDLLDDDARREE
                     LARRMGALATPGATGEVARRLLCAGEKE"
     gene            1193..2557
                     /gene="murC"
                     /locus_tag="VNT12_01560"
     CDS             1193..2557
                     /gene="murC"
                     /locus_tag="VNT12_01560"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWI44827.1"
                     /translation="MNIHMIGIGGAGMSGIAEVLGRRGHSVTGSDLKESPYTRRLRDA
                     GIMVYIGHAPDQVGDADRVVISTAIPKTNPELLQARRRSIPVIPRAAALAWILQGSRS
                     VAVAGTHGKTTTTSMTAHVLEALGENPTALIGGELNDIGSNVAFGRSDLVVAEADESD
                     RSFLYLKPQAAVVTNIEFDHPDFYSSLDDVVETFEQFVRSLPEDGHLVVCADDPSCPK
                     VAAEAPCPVTTYGIRSGDLRADIVKPSSYVLFEDGESRGEVELGVYGRHNVLNSLAAA
                     GVARWLGHQAYEAASTLQDFGGVRRRFQLKGEQAEVRVVDDYAHHPTELSATLAAARS
                     TMPGDGRVIAVFQPHRYSRTRALYREFGASFSSADAVLVTEVYGAGEMPQPGVNGKLI
                     VDAICETRGHPEVYYIPQQDAIPAVLQNVSRKGDLVLTLGAGDISKAGEDLLGILEEQ
                     GSKP"
     gene            2626..3225
                     /locus_tag="VNT12_01565"
     CDS             2626..3225
                     /locus_tag="VNT12_01565"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWI44828.1"
                     /translation="MTYLAFPVTGVRVEGARMYPESEVTDAIPDHASLLTLNEDLLKR
                     NLESHPWVQGTEVNEDWNSGIVTVQVEERRPVLDAEVDGRRFVLSADGEELPGPGGAG
                     LERVELDRDQVGEILAFVSTLRRSGLTLESIDGAGAGGIEATVEGREVIFSGNVGERR
                     VMALKHIMPEHPDDRVFDLRSPERVVVGAPHETRSEPEG"
     gene            3232..>3719
                     /locus_tag="VNT12_01570"
     CDS             3232..>3719
                     /locus_tag="VNT12_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWI44829.1"
                     /translation="MAQTLAYGIDVGTTKVVAIAGRVDPRRGWVEVLSMGEAPTYGLK
                     RGVVVDRKAAADSVAEAMEACGVRGGRVTVGIAGSHISSFNTEVTLLNRSRNLTITDR
                     FVRRLEQEARQLDLHEDERVIHIVPRGYVLDGSEGVKNPAGLAARKVTMRAHVVSGAV
                     SS"
BASE COUNT          690 a         1172 c         1219 g          638 t
ORIGIN      
        1 gctcgcaggt atgacgctcc ctttcgttag ttacggtgga tcgagtctgc tggtatgctt
       61 cgccgccgtg ggtatcctct acaggatatc ggaggacagt gaacgagcca gcgaaatcaa
      121 accacgcaag aatgccgcgg atcctgatag caggaggcgg cacgggggga cacgctatcc
      181 ccgcgctgtg cgtggcggct gatttgaaag agaggggagc gatagtcgag ttcgtgggat
      241 ctagctccgg catagaaatg tccctcgtcc cacagcaagg ctacaccctg cacgccctgc
      301 ccctgactgg cctcgccgga agcccgctgg cccaggcccg cgcgggcctc ctctccctga
      361 gagccgtcat ccgttgtaca cgcataatac aacgtctgag gccgctgtcc gtcctcggcg
      421 tcggaggcta cgcgagcgcg ccggcggtga ttgcggccag gacgctcggc gtgcccacgt
      481 tcctgcacga gcaaaactct gtccccgggc gggtcaaccg cctggccagc cgcttcacga
      541 cacagacatt agccacgttt cctgatgcca cgcggcggct tgggaacgcc gcgtgggtcg
      601 gtatgcctac caggaccgag atcttcgaag catcgcggga ggaagccctg cgggatctcg
      661 gcctgcagcc gccggtcgtt ctaatcttcg gcgggagcgg tggtgcgctc aggatcaacc
      721 tcgcggcggc ggaggcgttc gggggcacga cgccgtatac ggtcgtccag atctccggcc
      781 gcagggattt ctcacggctc catgcggaca acccgaggca cacgatcctc gagttcgcag
      841 gggatatctg gcgttacctg gccgccgctg acgtggtcgt tagcagggcc ggcgcgggct
      901 ctctgttcga cattgcggcc gccgggaagg cggcgatcct catccccttc ccctacgcga
      961 ccggcgacca ccagctcgtg aacgccaggt actttaccga caggggggcc gcggagttga
     1021 tgctagactc cgaggtcggc gcgcgtgaac ttcgcgaccg cgtcgaagac ttgttggacg
     1081 acgatgcacg gcgggaggag cttgccaggc gcatgggcgc gctcgctacg cctggcgcga
     1141 caggcgaagt agcccggcga ttgctgtgcg ctggagagaa ggagtagggc gcatgaacat
     1201 acacatgatc ggcatcggtg gggcaggaat gagcggcata gccgaggtcc tgggcagaag
     1261 gggccacagc gtcacgggct cggatctcaa ggagtctcct tacacccgca ggctgcgcga
     1321 tgcgggcatc atggtctaca tcgggcacgc cccggaccag gtaggggacg cagaccgtgt
     1381 ggtgatctcg acggccatac caaagactaa cccggagctt ctacaagcca gacgtagatc
     1441 catccccgtt atcccgcgcg ccgcagccct cgcctggatc ctccagggca gccgcagcgt
     1501 cgccgtggca ggaactcacg gtaagacgac gacgacgagc atgacagcgc acgtcctcga
     1561 ggctctaggt gagaacccga cggccctgat tggcggcgaa ctgaacgaca tcggcagcaa
     1621 tgtcgccttc ggaaggtctg acctcgtggt ggccgaggcg gacgagagcg accgttcctt
     1681 cctctacctc aagccacagg ccgctgtggt cacgaacatc gagttcgacc accctgactt
     1741 ctactcgtcc ctcgacgatg tcgtggagac cttcgagcaa ttcgtccgct ctctgcccga
     1801 ggacggccac ctggtcgtct gcgcggatga tccctcctgc cccaaggtgg ccgccgaggc
     1861 tccatgcccc gtaactactt acggcatccg ctcaggagac ctcagggcgg acattgtgaa
     1921 gccgagcagt tacgtgctgt tcgaggacgg tgagagccgc ggcgaggtcg aactgggcgt
     1981 ctacgggcgc cacaacgtgc tcaactccct ggcggcggcc ggagttgccc gctggctcgg
     2041 ccaccaggct tacgaggcgg ccagtaccct ccaggacttc gggggcgtgc gccgccgttt
     2101 ccagctcaaa ggagagcagg cggaggtacg cgtcgtcgac gactacgccc accatccgac
     2161 cgaactctcg gccacactcg ctgccgcccg ttccaccatg ccgggggatg gaagggtgat
     2221 cgccgtcttc cagccccacc gttactcacg tacccgcgcc ctctacaggg agttcggcgc
     2281 ctccttctcc tcggccgacg ccgtgctcgt caccgaggtc tacggcgccg gcgagatgcc
     2341 ccaaccaggc gtcaacggca agctgatagt cgacgcaatc tgcgagacgc ggggacaccc
     2401 cgaggtctac tacatacccc agcaagacgc tataccagcg gtgctgcaaa acgtctccag
     2461 aaagggagac ctagtgctca ccctcggcgc aggtgacatc tcgaaggccg gtgaagacct
     2521 gctcggtatt ctggaggagc aaggatccaa accctgacaa gatctctgcg tgccatcctg
     2581 atctcggttg ccacagccgc cggtactacc cttatcctca tcctcatcac ctaccttgct
     2641 ttccccgtta ccggcgtcag ggtggaaggc gcccgtatgt accccgaatc tgaagtcacc
     2701 gacgctatcc cagaccacgc cagcctgctc accctcaacg aagatttgtt gaaacgcaac
     2761 ctggagtctc atccttgggt tcagggtaca gaggtgaacg aagattggaa ctccggtata
     2821 gttacggttc aggttgagga gcgtaggcct gtgctggatg ccgaggttga cgggcggagg
     2881 ttcgttctct cggctgacgg cgaggagctt cctgggccag ggggggcggg tcttgagagg
     2941 gtggagttgg atcgcgacca ggtgggggag atcttggcgt tcgtgagcac gttgcgcagg
     3001 agcgggttga cactggagtc tatcgacggg gctggtgccg ggggcatcga ggccacggtg
     3061 gaaggccgcg aggttatctt ctccgggaat gttggtgaac gtcgggtcat ggccttgaaa
     3121 cacatcatgc cagagcatcc cgacgacagg gtcttcgacc tgcgttcgcc ggagagggtg
     3181 gttgtcggcg ccccgcacga aacgcgcagc gagccagagg gctagtcggc cgtggcgcag
     3241 acgctagcct acgggataga cgtgggcacc accaaggtag tggcgatcgc cggccgggtg
     3301 gacccgcgga ggggctgggt cgaagtccta tccatggggg aggcgccgac ctacggcctc
     3361 aagcgggggg tcgtggtaga ccgcaaagcc gccgccgatt cggtcgctga agccatggaa
     3421 gcctgcggcg ttcgcggcgg tagggtgacc gtcggcatcg cgggtagcca catctcttcc
     3481 ttcaacacag aagtcacgct cctgaaccgc agcaggaacc tgacgatcac agacaggttc
     3541 gtccgcaggc tcgaacagga ggcccgccag ctcgacctgc acgaggacga gcgggtgata
     3601 cacatcgtcc ctcggggcta cgtcctcgat gggtcagagg gcgtcaagaa tcctgcaggc
     3661 ctcgccgccc gcaaggtgac catgcgcgcg cacgttgtga gcggggcggt ctccagcat
//
LOCUS       DATMJA010000067         4100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_424369, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000067 DATMJA010000000
VERSION     DATMJA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424369"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VNT12_01575"
     CDS             complement(<1..633)
                     /locus_tag="VNT12_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44830.1"
                     /translation="MDHRVENTAIGREPLMVTKTQPPVLHEDFVPRYDLVQFLRKGVH
                     RKLTLLSAPTGSGKTMLLAEWSAADKEEHKFAWLSLDRQDDDPVRFWANLIGSLRVDT
                     PDLGARSLAALEAGADLTKFVLAPLVNELAALPTKIVLVLDDYHHIRERGCHEGIYFL
                     IAHAPHTLHVAISTRSEPPLPLGILRAQGQLTEIRGEGLRFTSEEASALLN"
     gene            complement(1003..1389)
                     /locus_tag="VNT12_01580"
     CDS             complement(1003..1389)
                     /locus_tag="VNT12_01580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HWI44831.1"
                     /translation="MAHKDFRILGLAGSLRRASFHRGILRAAHEVGPERMSCESFDMG
                     RIPYFNQDIEDEGNPEPVKELREKIRTHDAVLIATPEYDYAIPGVLTAALDWALRSRH
                     ERTPLRHKPVGIVGASPGGGGCNPSA"
     gene            complement(1426..1662)
                     /locus_tag="VNT12_01585"
     CDS             complement(1426..1662)
                     /locus_tag="VNT12_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWI44832.1"
                     /translation="MTERMVWRIAVIPGDGIGTEVVHEGQRVLDALAEDSGGAFAFEC
                     ESFLWGSQYYLENGRMMAEDALETRSKASEGALR"
     gene            2034..2963
                     /gene="ppk2"
                     /locus_tag="VNT12_01590"
     CDS             2034..2963
                     /gene="ppk2"
                     /locus_tag="VNT12_01590"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HWI44833.1"
                     /translation="MTKTKSKSDDKTKNKAKGKANRTAHIRMAQGTASADETVGKAGG
                     ELKRDKTGRIKRAAYEAELTRLQLELVKLQEYIKEAGLRVVVVFEGRDAAGKGGAIKR
                     IQEAMNPRVCRIAALAAPSDRERTQWYFQRYVAELPSAGEIVLFDRSWYNRAGVERVM
                     GFCTEEEYQEFLRATPLFEEMLIRSNIKLLKYWFSVSDEEQERRFQDRLSDPSKHWKL
                     SPMDLESRRRWVDYSRAKDVMFARTDTKLSPWWVVEADVKRHARLNAISHLLSSIPYE
                     YEPKPPLELPKRQDDPSYVRPPRETMRYVPEVY"
     gene            complement(3221..>4100)
                     /locus_tag="VNT12_01595"
     CDS             complement(3221..>4100)
                     /locus_tag="VNT12_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWI44834.1"
                     /translation="HDAPELRLASEMVAPLIPGGVVVPHEQEFATVGHLYRGIEEGFR
                     RLVEKYGEHRVFVGPPEAQATWRYFWWPELVPVTDLASAQAAIETIVEQGEGARSDRD
                     DAHFGMFLRVLVEYLEFKRRDPDFEPARPVTAAWVRPPVDAKLPNPTLITDPTTAGVA
                     DLFNATYEVMLQMLVRFFAHTEETEEELQTLSRTAVDAMSLLIKPLGQLLTTLPVGAE
                     YPGKSAGPNFEFYRTSYLLPHRHAAWVLMHERLLELAVYCARLGARPSAPPELTTIEE
                     TFRKLARAMEQHIGGR"
BASE COUNT          770 a         1312 c         1240 g          778 t
ORIGIN      
        1 gttcaacaga gcggaggcct cctcactcgt gaacctcagt ccttcgccgc ggatttcggt
       61 caactgaccc tgcgccctca gaatacctag aggcaaaggc ggctcagagc gggttgagat
      121 tgcgacgtgc agcgtgtgcg gcgcgtgggc tatcaagaag tagatgccct catggcatcc
      181 tcgttcccta atatggtggt agtcgtcgag taccagcaca atctttgtgg gcaacgccgc
      241 aagctcattg acgaggggtg ctagcacgaa ttttgtgaga tccgctcccg cctcgagcgc
      301 cgccaatgac ctcgccccga gatccggagt gtcgacgcgg agcgatccga tcaagttcgc
      361 ccagaagcga acaggatcgt cgtcctgccg atcaagcgag agccaggcga atttgtgttc
      421 ctctttgtcg gcggcgctcc attcggccaa gagcatcgtc ttcccgctac cggtcggagc
      481 actaagcagg gtaagctttc ggtgaacccc cttacgcaag aactgcacga gatcgtagcg
      541 aggcacgaaa tcttcgtgca gaaccggcgg ctgggtcttc gtaaccatga ggggctcgcg
      601 accgatagct gtgttctcta cgcggtggtc catttcgggg cctccactcc cgagctgtga
      661 gcatgcaatc atctgcacag ctcttgataa gaattataca tagaaaggtg tagagccata
      721 cttagtttgg ctgcccggga ccctagcgga gactgataac taggtcttat acctgagctt
      781 gagtttgctt tggcttcacc ttgcaggtat gcgacatcgt ctgcgccgac ttcgccgttc
      841 ctacccgatc gtggtgataa ggaatgccaa cgcgcacccc agggttgtca ctaggccgac
      901 ctgcttgccc ccgtacctga aggcctccgg catcatcgtg tcggccagca tggtcaagaa
      961 cgcgccgccc ggattgagat cgccaatgtg atggacaaga ctttaagctg acgggttgca
     1021 cccaccacct ccaggactcg cccccacgat ccctaccggc ttgtgcctaa ggggcgtgcg
     1081 ctcatggcgc gaacgcagcg cccagtccag cgcagcggtc aagacgccag ggatggcgta
     1141 gtcgtactct ggggtggcta tgagcacggc gtcgtgggtg cgtatcttct ctcttagctc
     1201 tttgacgggc tcggggttgc cctcgtcctc tatgtcctgg ttgaagtaag gtatccgtcc
     1261 catatcgaag ctctcgcagc tcatccgctc tggtccgacc tcgtgggctg ccctgaggat
     1321 gccgcggtgg aaggaggctc gcctgaggct gccggcgagg ccgagtatcc ggaaatcctt
     1381 atgggccatc gtttgcctcc cctgttggcc ttctcgatac cgactctacc tcaaggcgcc
     1441 ttccgaagcc tttgagcgcg tctccaaggc gtcctcggcc atcatgcgcc cgttttcgag
     1501 gtagtactgg gaaccccaga ggaacgactc gcactcgaag gcgaacgccc cgcccgagtc
     1561 ctctgccagg gcgtcgagca cgcgctgtcc ctcgtgcacc acctccgtgc caatcccgtc
     1621 accggggatc accgctatcc tccacaccat tctctccgtc atgaacccta acctccttta
     1681 ccggtcccta tggcatgacc agacaggtct gtcgcaggta cgatggcatc tccttacaca
     1741 tggcgccggt ggcacgtctt gaggacaggg acctggttca gcaccttctc gggcacgcct
     1801 cgataaccat gaccctcgac ccggcccata gaaaggccta cggacgcagg ccttcagcct
     1861 tctgccgaca cttgtggatc cggttcgtcg ccctatcagt tgcgtgttcg aaactcgtgc
     1921 gcgagcctcc tgcgctctgg tagccggaac gtcggtggtt acagaggttg tatgatatga
     1981 ctcgatatgg gaccatgctc gccaatcgct gacgggtgtc ggggagtaaa gctatgacga
     2041 aaacgaagag caaatccgac gacaagacca aaaacaaggc taaaggcaag gccaacagga
     2101 cggcccacat ccggatggcg cagggcactg cgtcggctga cgagaccgtc ggcaaggctg
     2161 gcggcgagct caagcgcgac aagacgggca ggatcaaacg cgcggcgtac gaggccgaac
     2221 tcacgaggct ccagctcgag ttggtcaagc tccaggagta catcaaggaa gcggggctgc
     2281 gggtcgtggt cgtgttcgag ggccgcgacg cagcgggcaa gggcggggct atcaagcgca
     2341 tccaggaggc gatgaacccg cgcgtgtgcc gcatcgccgc gctggcagcg ccgagcgacc
     2401 gcgagcgtac tcagtggtac ttccagcgct acgtggccga gttgccgtct gcgggcgaga
     2461 ttgtgctctt cgaccgctcc tggtacaacc gcgccggagt cgagcgcgtg atgggcttct
     2521 gcacggagga ggagtaccag gagttcctgc gggcgacccc actcttcgag gagatgctga
     2581 tccgctcgaa catcaagctc ctcaagtact ggttctccgt ctcagacgag gagcaggagc
     2641 gccgcttcca ggaccgcctc tccgacccga gcaaacactg gaagctcagc ccgatggacc
     2701 tggagtcccg ccggcggtgg gtggactact ccagggccaa ggacgttatg ttcgcgcgca
     2761 ccgacacaaa gctctccccc tggtgggtag tggaagctga cgtgaagagg cacgcgcggc
     2821 tgaacgcgat cagccacctg ctctccagta tcccctacga gtatgagccg aagccgccgc
     2881 tcgagctgcc caagcgccag gacgacccca gctacgtccg gccgccgcgg gagaccatgc
     2941 gctacgtgcc ggaggtctac tagagagatc accgtaagaa ccactctccg ggagcagcgc
     3001 ggcccatccc cgcccgaaag cccgagcgga gtccggtgcc ggcgccctcc tcgtactcct
     3061 ccctccaaac cgcacgaact gccacgcggc ccccggttca atacttgcgt atcgatctca
     3121 acgcatatca gccggctaat tgtgctccgg tggttctggc agctattgcg cagctcactt
     3181 tctttcagga cagtggtaag cgctcgtgga tacacctgcg ctagcgcccg ccgatgtgtt
     3241 gctccatcgc acgggcgagc ttccgaaagg tctcttcgat cgtcgtcagc tctggtggag
     3301 cggacggccg ggcgcccagc ctagcgcagt aaaccgccag ctcgaggagc cgctcatgca
     3361 taagcaccca ggcggcgtgg cggtgcggca gcaggtagct cgtgcggtag aactcgaaat
     3421 tcggccccgc actcttgcct ggatactccg ctcccaccgg cagggtagtg agaagttgtc
     3481 ccaaaggctt gatgaggagg gacatcgcgt ctacggcggt cctgctcaac gtctgtagtt
     3541 cctcttcggt ctcctcggtg tgggcgaaga agcggaccag catctgcagc atcacctcgt
     3601 aggtggcgtt gaacaggtca gccaccccgg ccgtggtggg gtcggttatc agcgtggggt
     3661 tgggcagctt cgcgtcgacc ggcggcctca cccatgccgc tgtcaccggt ctggccggct
     3721 cgaagtccgg atcccgcctc ttgaactcga ggtactccac caggacccgg agaaacatgc
     3781 cgaagtgggc gtcgtctctg tcgctccgag ccccctcgcc ctgctcgacg atcgtctcga
     3841 tcgccgcctg tgccgacgcg agatcggtga ccgggaccaa ctcgggccac cagaagtacc
     3901 tccaggtggc ctgggcttcg ggcggtccga cgaagactct gtgctctccg tacttctcga
     3961 ccaggcgcct gaagccttcc tcgatccccc ggtagagatg acccacggtg gcgaactcct
     4021 gctcgtgagg cacgacgacg ccgccgggga tgaggggcgc taccatctcg ctcgcgagcc
     4081 gcagctccgg cgcgtcgtgc
//
LOCUS       DATMJA010000068         4195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_720819, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000068 DATMJA010000000
VERSION     DATMJA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720819"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VNT12_01600"
     CDS             complement(<1..1376)
                     /locus_tag="VNT12_01600"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HWI44835.1"
                     /translation="MGLIPYKNEVYLDWSDEKNVEAMRAALKDVGARLDRSYPAIIGG
                     ERVETDGEIVSVNPADPSQVVGRTASATEREADMALDTATRAFETWSRTAPEARARIL
                     LRASAIMKRRKFELLAWEAYEGGKPWFEADAQVAEAIDFLEYYAREMLRLKDGVEIYS
                     VPGEESRYTYQPMGVGVIIAPWNFPTAILTGMSSAAIVAGNTIIMKPSEFTSVIGAVV
                     AEIFEEAGLPEGVLNFLPGYGSEVGDYLVEDARTRFISFTGSMKTGLRINELAARQIE
                     HQRWVKRVIAEMGGKDAMVIDDSADLDSAASDIVKSAYGYSGQKCSAASRAILHADVY
                     EEVLDKVVEKAKDLKIGGPESADVNVGPVISEPQFEKVAGYVDVGKQEGERVLGEDPG
                     DPQSGYFISPTIFEVEREARVAREEIFGPVLSVIKASDFDEALRVANDSPYGLTGGVY
                     SKNRDHLER"
     gene            complement(1386..2303)
                     /locus_tag="VNT12_01605"
     CDS             complement(1386..2303)
                     /locus_tag="VNT12_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWI44836.1"
                     /translation="MGIVDKAIASSVPAIPRPIVKRISSRYIAGDTLDEAISTVRDLN
                     EGGCVATIDLLGESTESKANATATLRDYEKVVDALDAHGLDSGISVKLTGLGLTLNEQ
                     LCRANLEEIVEYAKERGRFVRVDMEDSPYTEATLRIVEDTHARHGNTGAVLQAYMRRS
                     IEDVQRIIDAGFSVRLCKGIYDEPRSIAYKDFDTIRQNYIFLLDELLKGGVYVGIATH
                     DEFLIWHALRLIHQLEVPEDRYEFQMLLGVDEELRSILVGAGHKLRVYVPFGEDWYEY
                     STRRLKENPKIAGYVARDVIGGITAKVKR"
     gene            2482..4041
                     /locus_tag="VNT12_01610"
     CDS             2482..4041
                     /locus_tag="VNT12_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWI44837.1"
                     /translation="MASIIGGRAPDGAPGGRMTSTNPALTSETVAEVSLGDAGTFVEA
                     CRAAREAQREWAAVPAPIRSQVVKKIGRVVENNKEALARLVTREIGKPYAESLGEVQE
                     IIDTCDFFTGEGRRLYGQTVPSEMPDKQLFTFRVPIGVAAIITAGNFPVAVPSWYLIP
                     ALTCGNAVVWKPAEYSPAIGDALAKLFMAGGLPDGVLNVVQAEGPAVFEGLEKALDEG
                     LVDKVGFTGSSEVGKRIGELCGRHMQSPCLELGGKNPLVVMPDADLELAVEGALFSGF
                     GTAGQRCTSLGTVIVQRSVHDEFVSRFTRAVEESPIGDPTQDVLYGPMLSERFHERFL
                     GWLELIEPHHSIHGSSGMGRISAENPREGFVGDPGAGIYDHPTIVDGVTMEDEIYSTE
                     TFGPIVGVASFSDFDEAIALSNGHGYGLSAAIYTTDATNALRFRERNGAGMLSVNNST
                     SGAEAHLPFGGNGKSGNGSRLSGIWVLDQFTRWQSMNWDYAGKLQKAQMDIADLPADL
                     DYTLPQEDKGV"
     gene            4041..>4195
                     /locus_tag="VNT12_01615"
     CDS             4041..>4195
                     /locus_tag="VNT12_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI44838.1"
                     /translation="MYDVIVAGLGGMGSSAAYHLAGRGERVLGLERHTPAHDKGSSHG
                     QSRIIRLA"
BASE COUNT          728 a         1371 c         1283 g          813 t
ORIGIN      
        1 cgctcgaggt ggtcgcggtt cttggagtag acgccgcctg tgaggccgta ggggctgtcg
       61 ttggcgacgc ggagggcttc gtcgaagtcg ctggccttta tgacggagag gacggggccg
      121 aagatctcct cgcgcgcaac cctggcctcg cgctcgacct cgaagatggt cgggctgatg
      181 aagtagccgc tctgcgggtc tcctgggtct tcgccaagga cgcgttcgcc ctcctgcttg
      241 ccgacgtcta cgtagccggc gaccttctcg aactgaggct cgcttatgac gggtccgacg
      301 ttcacgtcag cgctctccgg gccaccgatc ttgagatcct tcgccttctc gacgacctta
      361 tccagaacct cctcgtagac gtcggcgtgc aggatagccc ggctcgcggc gctgcacttc
      421 tggcccgagt agccgtaggc gctcttgacg atgtccgagg cggcggagtc gaggtccgcc
      481 gagtcgtcta tcaccatcgc atccttgcca cccatctcgg ctatgaccct cttgacccag
      541 cgctggtgct ctatctgtct ggccgccagc tcgttgatgc ggagacccgt cttcatcgag
      601 ccggtaaagg agataaagcg ggtccttgca tcctccacca gataatcccc tacctccgag
      661 ccgtagccgg gcaggaagtt caggacgcct tctggcaggc cagcctcctc gaaaatctcg
      721 gccaccacgg cgccgatgac gctcgtgaac tccgagggct tcatgatgat ggtgttcccc
      781 gcgacgatgg cagcgctcga catccccgtg aggatggcgg tagggaagtt ccagggagcg
      841 atgatgaccc cgacgcccat cggctggtag gtgtagcggc tctcctcgcc ggggacggag
      901 tagatctcga ccccgtcctt gagcctcagc atctcgcgag cgtaatactc gaggaaatct
      961 atggcctcag ccacctgcgc gtcggcctcg aaccagggct tgccgccctc gtaggcctcc
     1021 caggcgagca gttcgaactt gcgccgcttc atgatcgccg atgccctaag cagtatgcgc
     1081 gccctcgcct ccggcgccgt acggctccag gtctcgaagg ctctggtagc cgtatcgagc
     1141 gccatgtccg cctcacgctc ggttgcactg gcggtgcggc cgacgacttg cgaagggtct
     1201 gcagggttga cagagacgat ctcgccatcc gtctccacac gctcgccgcc tatgatcgcg
     1261 ggatagctcc ggtccagtcg ggcgccgaca tctttcaggg cggcccgcat agcttcgacg
     1321 ttcttctcgt ccgaccagtc aagatacact tcgttcttgt acggtatcag tcccacgtaa
     1381 ggctcctatc tcttcacctt ggcggttatg cctccgatca cgtccctcgc aacgtagccg
     1441 gcgatcttgg ggttctcctt gagacgcctg gtcgagtact cgtaccagtc ctccccgaaa
     1501 ggcacgtaga ccctgagctt gtgcccggcc ccgaccagta tgctcctcag ttcctcgtct
     1561 accccgagga gcatctggaa ctcgtagcgg tcctcgggga cttcgagctg gtggatgagc
     1621 ctgagggcgt gccagatcag gaactcgtcg tgcgtcgcta tgccgacgta gacgccgccc
     1681 tttaagagct cgtcgagcag gaaaatgtag ttctggcgga tggtatcgaa atccttgtag
     1741 gctattgatc tcggctcgtc gtagatgccc ttgcacagcc gcacggagaa acccgcgtcg
     1801 atgatgcgct gcacgtcctc gatgctgcgg cgcatgtaag cctgcagaac cgctcccgtg
     1861 ttcccgtgcc tggcgtgcgt atcctcgacg atccgcagcg tcgcctccgt gtaaggcgag
     1921 tcctccatat cgacccgcac aaaacggccc cgttctttgg cgtactcgac gatctcctcc
     1981 aggttcgccc tgcagagctg ctcgttcaag gtcagcccga gcccggtaag cttgaccgag
     2041 atgccgctgt cgaggccatg cgcgtcgagc gcgtcgacga ctttttcgta gtccctgagc
     2101 gtcgccgtcg cgttggcctt gctctcggtg ctctcgccga ggaggtcgat ggtggcgacg
     2161 cagccccctt cgttcaggtc tctgaccgtg gagatcgcct catccagcgt atcgccggct
     2221 atatagcggc tggagatcct cttcactatg ggccgcggga tggctggcac cgagctcgct
     2281 atggctttgt ccactattcc cacgtctcac atccccacaa aacgacctgt atggcgccct
     2341 atataccctg attttactgt aaggagcgtt ccgaggcttt ggtgtaggat agtaccaaga
     2401 taggcgaacg agataggcct ggaggcagag agaatgcgta ccaccgatgc acgtgaagga
     2461 acagcttcag agacccgtac cgtggcctcg ataataggag gccgcgctcc cgacggcgcg
     2521 cccggcgggc gtatgacttc gacgaacccg gcgcttacgt ccgagacggt ggctgaggtg
     2581 tcgctcgggg acgcaggaac gttcgtggag gcgtgtaggg ctgctcgtga ggcgcagcgg
     2641 gagtgggccg ctgtgcctgc ccccatccgc tctcaggtag ttaagaaaat cgggcgcgtc
     2701 gtcgaaaaca acaaggaggc gctcgcgcgg ctggtgaccc gtgagatcgg caagccttac
     2761 gcggagtctc tcggcgaggt gcaggagata atcgacacct gcgacttctt taccggtgag
     2821 ggccgtagac tctacggcca gaccgtcccc tcggagatgc cagacaaaca gctcttcacc
     2881 ttccgcgtcc ctatcggcgt cgcggccatc atcacggccg gcaacttccc ggtggcggta
     2941 ccttcgtggt acctgatccc cgccctcacc tgcggcaacg cggtggtctg gaagcccgcc
     3001 gaatactccc ccgccatcgg tgacgccctg gcaaagctct tcatggctgg cggcctcccc
     3061 gacggcgtcc tcaacgtcgt gcaggccgaa ggaccggcgg tcttcgaggg gttggagaag
     3121 gccctcgacg aggggctcgt cgacaaggtc ggcttcacag gctcttccga ggtcgggaag
     3181 aggataggcg agctctgcgg caggcacatg cagtctccct gcctggaact cggaggcaag
     3241 aacccgctcg tcgtcatgcc cgacgcagac ctcgagctcg ccgtcgaagg cgccctgttc
     3301 tcgggcttcg gcacagcggg tcagcgctgc acctctttgg gcaccgtgat cgtccaacgg
     3361 tccgtccacg acgagtttgt gtccaggttc actcgtgccg tcgaggagtc acccatagga
     3421 gacccgacgc aggacgtgct gtacggacct atgctcagcg agcgcttcca cgagcggttt
     3481 ttgggttggc tcgagctgat cgagccccat cacagcatcc acggttcgag cgggatgggc
     3541 aggatctccg cggagaatcc ccgcgagggc ttcgtcggag atccaggcgc cggtatctac
     3601 gaccacccga ccatcgtcga cggcgttacg atggaggatg agatctacag cacagagacc
     3661 tttggtccca tcgtcggcgt cgcctccttc tcggacttcg acgaggccat agccctctcc
     3721 aacgggcacg gctacgggct ctcggccgcc atctacacca ccgacgcgac aaacgcgctg
     3781 cgcttcaggg agcgcaacgg cgcggggatg ctctccgtca acaactccac cagcggcgct
     3841 gaggcccacc tgccctttgg gggcaatggc aagagcggaa acgggagccg tctctcgggc
     3901 atctgggtgc tcgaccagtt cacccgctgg cagtccatga actgggacta cgccggcaaa
     3961 ctccagaagg cccagatgga catagccgac ctccccgccg acctcgacta caccctgcct
     4021 caggaagaca aaggcgtctg atgtacgacg tgatcgtcgc tggcctcggc gggatgggga
     4081 gctctgctgc ttaccacctc gccggacgcg gagagcgcgt gctcgggctg gagcggcaca
     4141 cccctgcgca cgataagggg tccagccacg gccaatccag gatcatccgc ctggc
//
LOCUS       DATMJA010000069         2667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_128516, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000069 DATMJA010000000
VERSION     DATMJA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128516"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VNT12_01620"
     CDS             <1..1095
                     /locus_tag="VNT12_01620"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWI44839.1"
                     /translation="VTRLEGAMPPSVDADKRVMLYRVPRGVVGVISPWNWPYTMPAEL
                     IAPALACGNAVVWAPASSTSVCAVKLAECIVDAGLPSGLFNMITGPGPVVGDEVAANP
                     GTQAVGFIGSTETGQKVAGRAAGKTLLLEMGGNGPMVILDDADLDAAAEASLTSAFLG
                     AGQSCTAGERFLVHERVYDAYLEGLGGAIERGIILGDPFDPITTLGPLNNEPTADKMD
                     LHISDALEGGAELISGGARAEGFPTDLYYRPTVLGKVTAEMRVSREETFGPIVPATVI
                     SDEEEAIKAVNASPYGLLSAVFTRDLARGLRFAEAVRAGWVNINASSNHWESHLPFGG
                     RAGSRSGVGRVGGRYAMETFTELKTVIVSL"
     gene            complement(1135..1914)
                     /locus_tag="VNT12_01625"
     CDS             complement(1135..1914)
                     /locus_tag="VNT12_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44840.1"
                     /translation="MTSKEQGTEDIRADMVEEAVRVAEEAERNDVALRLLGGVAIKVR
                     AHDGLEPAFEREYADLDWITQKGKSTQAQRFFESLGYVPQTRFNAMYGRERLLYFDEK
                     HDRQVDVLVGNFRMCHEIPFGDRMTMEPLTVPLAELLLTKLQIIELNEKDVGDALALL
                     HDHPVGEQDGDTINAAHIAKLCASDWGLWRTFTANLEALDGYLNRFDLKDESKERITE
                     RVRALLARIEEEPKTFGWKMRSKIGDRKRWYDLPEEVEGGP"
     gene            complement(1914..>2667)
                     /locus_tag="VNT12_01630"
     CDS             complement(1914..>2667)
                     /locus_tag="VNT12_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI44841.1"
                     /translation="KVLLEEELGYDNVELKLADDVGPVYKELIESETDAFLDAWMPNH
                     EQFVEGGRGRIEVSKEPWYVDPTRYGIAVPDYMQDVRSISELDSSGTDMITGIEPGAV
                     LMKKIETDVVPRYDLQSSLVGATTPAMLAELEQAYSMEEPFVFLAWSPHWMNQEYEFR
                     YLSDPKNAMGSVDAPQTLHSVTREGLAEDEPAAYALINAMRLDEDQIGSLELAINKAE
                     DPEKGVTRWLEEKENRKLVRPWIEAAKNAQEE"
BASE COUNT          443 a          874 c          816 g          534 t
ORIGIN      
        1 gtgacgcggc tggagggggc catgccgccc tcggtagacg ccgacaagcg cgtcatgctc
       61 tacagggttc cgcgcggggt agtcggcgtg atctcaccgt ggaactggcc ctacaccatg
      121 ccggcggagc tgatcgcgcc ggcgctagct tgcggcaacg cggtcgtctg ggcgccggcc
      181 tcctcgacgt ctgtctgtgc cgtgaagctc gccgagtgca tcgtcgacgc gggactgcca
      241 tccgggctct tcaacatgat caccggcccc ggtcccgtcg tcggagacga ggtggcggcc
      301 aacccaggca cccaagcggt cggttttatc ggctccaccg agaccggaca gaaggtggcc
      361 gggcgtgccg ccggcaagac gctactcctc gagatgggag gcaacgggcc gatggtgatc
      421 ctggacgatg ccgacctcga cgccgccgcg gaggcgagcc tcacctccgc ctttctgggc
      481 gccggtcaga gctgcaccgc cggggaacgg ttcctggtgc acgagagggt ctacgacgcc
      541 tacctcgaag ggctcggcgg ggcgatcgag cgtgggatca tactcggcga ccccttcgac
      601 ccgatcacca cgctcggccc cctcaataac gagccgacgg ccgacaagat ggatctgcac
      661 atctccgacg cgctcgaggg tggcgcggag ttgatctccg gcggggcccg cgccgagggt
      721 ttccctacgg atctctacta ccgcccgacc gtgttgggca aggtcaccgc tgagatgcgc
      781 gtctcgaggg aggagacttt cggacctata gtcccagcga ccgtcatcag cgacgaggag
      841 gaagccataa aggccgtcaa cgcctccccc tacggcctgc tctccgcggt ctttacccgg
      901 gatctggcga ggggcttgcg cttcgccgag gcggtgaggg ccggctgggt caacataaac
      961 gcctcatcca accactggga gagccacctt cccttcggcg ggcgagctgg ctcccgctcg
     1021 ggtgtcggac gtgtcggagg acgctacgcg atggagacct ttaccgaact caagaccgtc
     1081 atcgtgagct tgtaatagtg gagatcggcg gggtgcacgc gttcgatcca gagatcacgg
     1141 ccctccctcc acctcctcgg gcaggtcgta ccagcgtttg cgatcaccta tcttcgaacg
     1201 catcttccac ccaaaggtct tcggctcttc ctcgatgcgt gcgagcaacg ccctgacacg
     1261 ctccgtgatg cgctccttgc tctcgtcttt caggtcgaag cgattcaggt aaccgtccag
     1321 ggcttcgagg ttggccgtga acgtgcgcca caggccccag tcggaggcgc acagtttggc
     1381 gatatgggca gcgtttatcg tgtctccgtc ctgctcgccc acgggatggt cgtgcaagag
     1441 ggcgagggcg tcgccgacat ccttctcgtt gagctcgatg atctggagct tcgtaagcag
     1501 aagctcagcg agtgggacgg taagcggctc catggtcata cggtccccga aggggatctc
     1561 gtggcacatc ctgaagttgc cgacaaggac gtcgacttgc ctgtcgtgct tctcgtcgaa
     1621 gtaaagtagc cgctcccggc cgtacatcgc gttgaaacgt gtctgcggga cgtatcccag
     1681 ggactcgaag aaccgctgcg cctgggtaga tttacccttc tgagtgatcc aatccaggtc
     1741 agcgtactcc cgctcgaagg cgggctccag gccgtcgtgt gcacgtactt ttatggccac
     1801 ccctccaagc aggcgaagcg ccacgtcgtt gcgctcggcc tcctcagcga cgcgcacggc
     1861 ctcctcgacc atatcggccc tgatatcttc cgtgccctgc tctttgctcg tcactactcc
     1921 tcctgcgcgt tctttgcggc ttcgatccag ggccgcacca gtttcctgtt ctccttctct
     1981 tcgagccacc gggtgacgcc cttctccgga tcttcagcct tgtttatagc cagctcgaga
     2041 ctgcctatct ggtcctcgtc gagcctcatg gcgttgatga gggcgtaggc tgccggttca
     2101 tcctcggcga gcccttctcg cgtgaccgaa tgcagcgtct ggggggcgtc caccgacccc
     2161 atcgcgttct tcgggtcgga gaggtaacgg aactcgtact cctggttcat ccaatgcggc
     2221 gaccaggcga ggaacacgaa cggctcttcc atgctgtacg cctgctctag ctcggccagc
     2281 atcgcaggcg tggtggcccc gacgaggctg gattgcaaat cataccgcgg gaccacgtcg
     2341 gtctcgatct tcttcatcag gacggcaccg ggttctatgc ccgtgatcat gtcggtcccc
     2401 gaggagtcga gttcggagat actacgcacg tcctgcatgt aatcggggac ggctatgccg
     2461 tacctggtcg gatcgacgta ccacggctcc ttggagacct cgatccggcc cctacccccc
     2521 tctacgaact gctcgtggtt cggcatccag gcatccagaa aagcatcggt ttcgctttcg
     2581 atcagctcct tgtagacagg cccgacatcg tcggcgagct tgagctcgac gttatcgtag
     2641 ccgagttcct cttcgagcag caccttc
//
LOCUS       DATMJA010000070         4174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_365588, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000070 DATMJA010000000
VERSION     DATMJA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365588"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VNT12_01635"
     CDS             <1..355
                     /locus_tag="VNT12_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWI44842.1"
                     /translation="ANGKVLLAFTEDSAEEVSLQTPLEAFTRQTITKVRDLRSELGRV
                     RAEGWASTLGELEDGFNGVAVPVSDALGRCRASLSVSGPVYRMPPARLPEIAVLCKKA
                     AGEISASLGKRRSSD"
     gene            634..1608
                     /locus_tag="VNT12_01640"
     CDS             634..1608
                     /locus_tag="VNT12_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44843.1"
                     /translation="MISKLFSREPDTKVFFATDIHGSETCWRKFLNSGKHYEANIMIL
                     GGDMTGKALVPIVEEGPNRWHATLLENRREFESEDEVKEFEDSVKRRGYYPFRTDPDE
                     MSELESDEDLRNELFYKEMLGTIERWMQMADEKFEGTDIECFVSPGNDDQFEVDDIIS
                     SAKRVRLAEGKVVEFGDYQMVSTGWSNRTPWDTYREEDEEDLAARLKKMTDQVTAPPE
                     KTIYNFHCPPYKSGLDDAPEIDENMRPKHGGRSIVPVGSKAVREAIEEGQPALALHGH
                     IHEAKGNTRIGRTLCINPGSSYEQGQLLGAVVNLDGGKKVKRFVLTSG"
     gene            1622..3232
                     /locus_tag="VNT12_01645"
     CDS             1622..3232
                     /locus_tag="VNT12_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWI44844.1"
                     /translation="MASNSTADSGAGDLFQRKATGLLKGWSVLDGFIYASFAINLITL
                     GLYIFSFAPFIPEGSLVYAIILSGLYLVFQAVTYASLIAVMPRAGGDYVWMSRVLGGG
                     IGFMLAVTGWWFILWHWVPIYANILNIEVLVPLATIAGFDGVANFFGVPGGMFTASMV
                     VAILASILISLGMRTYARIQKFCFYGGVIGLVIMLALLLFNSNADFVGAFNSQAQDVY
                     GAGGDAYQRTLDAGSVKGTNLGALAFGPTLLLIPMIVFFNLWSNWGATLYGEVRGASD
                     FRKNIYAMGGALIVTSAVAVIMLLLFAKTFGWDFYYAASNAYWGGNGPLSVWPYPGLF
                     AAFLFDSPILQFILVALLALWFFGWVGSVFLSSTRVVFATAFDRVLPEWVAKTSRNGV
                     PYYALMLMLLPSIPISYLYAYGENFATYTLDATLVIATTFLGSAIAAAVLPWRKPEIY
                     NASPIARYNVLGLPLITVAASMFSIFLVFCIYKWLTDDTYGINNGQSLIYMMILYVVA
                     LVLYVGFRLLRRAQGMDLKMVYDEIPEE"
     gene            complement(3261..>4174)
                     /locus_tag="VNT12_01650"
     CDS             complement(3261..>4174)
                     /locus_tag="VNT12_01650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HWI44845.1"
                     /translation="VKTPENLAYLRPETAQGIFVNFKNVLQTSRVKVPFGIAQIGKSF
                     RNEITPGNFIFRTREFEQMEMEFFVEPGTDEAWHEYWIEERWNWYTNLGLKEENLRRY
                     EHPKEKLSHYSKRTVDLEYNYASVGWSELEGIANRTDFDLKQHAAYSGETLEYFDQAT
                     GERYVPYVIEPAVGPDRIMLAFLVDAYTEEEVRGEGRTLLKLHPRIAPTKAAVFPLSK
                     KEPVSTIARGLYDDLKGDYRLFYDDSGSIGRRYRRQDEAGTPFCVTVDFDTIEDKQVT
                     IRDRDTLEQERVPLAAVRDRLKALISG"
BASE COUNT          775 a         1245 c         1288 g          866 t
ORIGIN      
        1 cgccaacggc aaggtgctgc tggctttcac ggaggattcg gccgaggaag tatccctcca
       61 gacccccctg gaggcgttca cccggcagac gatcacgaag gtcagggatc tccgctccga
      121 gctcggacgc gtcagggcgg agggttgggc gtcgaccctg ggagagcttg aggacgggtt
      181 caacggcgtt gcggttcctg tctccgacgc gctcgggcgc tgccgggctt cgttgagcgt
      241 gtcggggccg gtctatcgaa tgcccccggc gcgtttgccc gagatcgcgg tactctgcaa
      301 gaaggccgcc ggggagataa gcgcttctct aggaaagagg cgtagctcgg actgaaacgc
      361 gcggcacagg cgtcttgcgt ttactactgg tcgtcgggtt gtcggcagtc ggattacctc
      421 agcgttgcgg agcttctatg caacaggtgg ccaacagtat agaatagggc gataggctgt
      481 acgttgacgg ggcagcggag tctggggaaa ggcgaagatc gcagcgtgag ttcggacgaa
      541 gaacgagccg aagtcctacg cctagaagat tgttcgcgcg atacacgatc gcaaacgctg
      601 gtacgagctg cctgaggagg tggacggagg accatgatct ccaaactttt ttcacgggaa
      661 cccgatacca aggtgttctt cgctacggac atccacggct cggagacctg ctggcgcaag
      721 ttcttgaact cgggcaagca ctacgaggcg aacatcatga tcctcggtgg cgatatgaca
      781 ggcaaggcgc tcgtcccgat agttgaggaa ggacctaacc gctggcacgc gacgctactc
      841 gagaaccggc gggaattcga gagcgaagac gaggtaaagg agttcgagga ctcggtcaag
      901 aggcgcggct actacccttt ccgcaccgac ccggacgaga tgagcgagct cgagtcggat
      961 gaggatctgc gcaatgagct cttctacaag gagatgctgg gaaccataga gcgctggatg
     1021 cagatggccg atgagaagtt cgaaggcacc gacatcgagt gcttcgtcag cccaggcaac
     1081 gatgaccagt tcgaggtcga tgatatcatc tcctccgcca aacgcgtacg gctcgccgag
     1141 ggcaaggtcg tcgagttcgg cgactaccag atggtctcta ccgggtggtc gaaccgtacg
     1201 ccctgggata cctatcgcga agaggacgag gaggacctcg cagcacgcct gaagaagatg
     1261 acggaccagg tcaccgcccc tcccgagaaa acgatctaca acttccactg cccgccctac
     1321 aagtccgggc tcgacgacgc ccccgagata gacgagaata tgcgccccaa gcacggtgga
     1381 cgctcgatcg tacctgtcgg ctcgaaggcg gtgcgggagg cgatagagga gggacaaccg
     1441 gccctggctt tgcacggtca catccacgag gccaagggca acacccgcat cgggcgcacg
     1501 ctgtgcatca atcccggcag ttcatacgag cagggacagt tgctcggggc cgtcgtcaac
     1561 ctcgacggcg gtaagaaagt caagcggttc gtactcacca gcggatagaa ggaggagatt
     1621 aatggccagc aactccacgg cggactctgg cgcaggagac ctgttccaga ggaaggcgac
     1681 cggccttctc aaaggatggt ctgtgttgga cggctttatt tacgcgtcgt tcgcgataaa
     1741 cctcattaca ctcggcctct acatcttctc cttcgcgccc tttatccccg aggggtctct
     1801 agtctacgcg atcatactct ccggactcta cctcgtcttc caggctgtga cttacgcttc
     1861 cctgatcgcc gtgatgcccc gggccggcgg cgattacgtg tggatgagcc gtgtcctggg
     1921 cggtgggata ggctttatgc tcgccgtaac gggctggtgg ttcatactgt ggcactgggt
     1981 cccgatctac gcgaacatcc tgaatatcga ggtgctcgtc ccactcgcga cgatcgcagg
     2041 gttcgacgga gtcgcaaact tcttcggggt gcctggtggg atgttcacag cctcgatggt
     2101 cgtcgcgatc ctggcttcca tcctgatctc gctcggcatg agaacctacg cccgcatcca
     2161 gaagttctgc ttttacggcg gggtcatcgg gctcgtgatc atgctggccc tcctgctctt
     2221 caactcgaac gcggatttcg tcggcgcctt taactcccag gcccaggacg tctacggcgc
     2281 ggggggcgac gcctaccagc ggaccctcga cgccggaagc gtcaagggaa ccaatctcgg
     2341 ggctctcgcc ttcggcccga cgttgctcct gatcccgatg atcgtcttct tcaacctgtg
     2401 gtctaactgg ggagccacgc tctacggcga ggtccgcggc gcctcggact tccgcaagaa
     2461 catctacgcg atgggcggcg cccttatagt cacctcggcc gtcgccgtca tcatgctgct
     2521 tctcttcgcc aagacgttcg gatgggactt ctactacgcg gcgagcaatg cttactgggg
     2581 cgggaacgga ccgctttcgg tctggcccta ccctggactc ttcgcggcgt tcctcttcga
     2641 cagtcccatc ctacagttca ttctcgtggc gctgctcgcg ctgtggttct tcgggtgggt
     2701 cgggtctgta ttcctctcct ctacgcgcgt cgtcttcgcg acggccttcg accgggtgtt
     2761 gcccgagtgg gtcgccaaga cctcgcgcaa tggcgtcccc tactacgcgc tgatgctcat
     2821 gctcttgccg tcgatcccga tcagctactt atacgcttac ggggagaact tcgcgactta
     2881 tacgctcgac gcgacgttgg tcatagcaac aaccttcctc ggctcggcca tcgcggctgc
     2941 ggttctgccg tggcgtaagc ccgagatcta caacgcctct ccgatagccc gctacaacgt
     3001 gctcggcctc cccctgataa ccgtagcggc gtccatgttc tcgatcttcc tcgtgttctg
     3061 catctacaag tggctcaccg acgacaccta cggcatcaac aacggccaat cgctcatcta
     3121 catgatgatc ctctacgtcg tggcactggt actgtacgtc ggcttccgcc tgctccgccg
     3181 cgcccagggg atggacctga agatggtcta cgacgagata ccggaggagt agccacctcg
     3241 gtatccgacg gctgaaggtc ctagcccgag ataagggcct tcagcctgtc ccttaccgca
     3301 gcgaggggta cgcgctcctg ttccagggtg tcgcggtcgc ggatggtcac ttgtttgtct
     3361 tcgatagtgt cgaagtcgac cgtgacgcag aagggggtgc ccgcctcatc ttggcgcctg
     3421 tagcggcggc ctatggagcc tgagtcgtcg tagaagaggc gatagtcgcc tttgaggtcg
     3481 tcgtagagcc cacgggctat ggtcgagacg ggctctttct tggagagcgg gaagacggcg
     3541 gccttggtcg gggctatgcg tgggtgtagc ttgagaaggg tacgcccctc gcctctcacc
     3601 tcttcctcag tgtacgcgtc gacgaggaag gcgagcatga tcctgtccgg accaacagca
     3661 ggttctatga cgtaggggac gtagcgctcg ccagtggcct ggtcgaagta ctccagggtc
     3721 tcgccggagt atgcggcgtg ctgcttcaag tcgaagtccg tgcggttggc tatcccttcg
     3781 agctcggacc accctacgga ggcgtagttg tactcgaggt ccacggtacg cttggagtag
     3841 tgggagagct tctccttggg gtgctcgtag cggcggaggt tctcctcttt caggcccaag
     3901 ttcgtgtacc agttccagcg ctcctcgatc cagtactcgt gccaggcctc gtccgtgcct
     3961 ggttcgacga agaactccat ctccatctgc tcgaactcgc gggtacggaa gatgaagttg
     4021 ccaggtgtga tctcattccg gaaagacttg ccgatctggg ctatcccgaa gggcaccttt
     4081 accctgctgg tctggagcac gttcttgaag ttcacgaaga tcccctgcgc cgtctcgggc
     4141 cgcagatagg ccaggttctc aggggtcttg acgg
//
LOCUS       DATMJA010000071         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_484124, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000071 DATMJA010000000
VERSION     DATMJA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484124"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VNT12_01655"
     CDS             complement(<1..502)
                     /locus_tag="VNT12_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWI44846.1"
                     /translation="MKAVVWHGKEDVRVDNVPDPIIQESTDAIVRITSTAICGSDLHL
                     FAKLSPLMREGDIIGHEPIGIVEEVGSDVEHISPGDRVVIPFNISCGHCYFCDQELYS
                     QCENTRDEGKLAKTVNLLGRGRGASLFGYTHVYGAVPGGQAEYLRVPQAHFGPIKVPE
                     GPPDERF"
     gene            complement(499..1554)
                     /locus_tag="VNT12_01660"
     CDS             complement(499..1554)
                     /locus_tag="VNT12_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI44847.1"
                     /translation="MRIVVVGATGNIGTSVLRSLEREEGVESVLGLARRLPGLSMPKV
                     EWASADVVDDDLVPHFTGADAVVLLAWLIQPSRDLTKQWMVNVEGSTRVARAAKEAGV
                     PSLVYASSVGAYSAGPKDRRVDESWPTGGVPSSYYGRHKAEVERRLDRFEAETPDVRL
                     VRMRPGLVFKREAAVGIRRLFAGPFFPSVLANPALINVVPDIENLRSQVVHSYDVGEA
                     FRLAIVNEDARGPFNLAAEPVLDANEIGRILNARPVPISAQLVRAGAGLSWALRLQPV
                     PPGWLDLSRNVPIMDTTRARTELGWTPQHTADEALLDLLEGLRTGSGIDTPPLSPKTG
                     GRFRIREMLTGVGGREP"
     gene            1641..1781
                     /locus_tag="VNT12_01665"
     CDS             1641..1781
                     /locus_tag="VNT12_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI44848.1"
                     /translation="MRVLVTGARGKVGRATVAAFQDAGHDVRATDLTPPVFERPEEGE
                     AD"
     gene            complement(1803..2093)
                     /locus_tag="VNT12_01670"
     CDS             complement(1803..2093)
                     /locus_tag="VNT12_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44849.1"
                     /translation="MLEYDDERSGDFGPLSHVPGDKVAVLGLVTTKTPRRETKQELEA
                     RLREASRFIPLERLALSPQCGFATSIIGNAISVEDEEYKLRTIVETAEQIWG"
BASE COUNT          403 a          725 c          748 g          411 t
ORIGIN      
        1 ggaatcgctc gtccgggggt ccttcgggca ctttgatcgg tccgaagtgg gcctggggca
       61 ctcgtaggta ctcggcctgc ccgccaggga cggcgccgta gacgtgggtg tatccgaaga
      121 ggctcgcccc cctgccgcgt cccaggaggt tcacggtttt ggcaagcttg ccctcatcgc
      181 gcgtgttctc gcactgggag tagagctcct ggtcgcagaa gtagcagtgc ccgcaagaga
      241 tgttgaaggg gatcacgacg cggtcaccgg gcgagatgtg ttcgacgtca gagccgacct
      301 cctcgactat acctatcggc tcgtgcccga tgatgtctcc ctcgcgcatc aacggcgaga
      361 gcttggcgaa gaggtgcaga tccgagccgc agattgctgt cgaggtgatc cttactatgg
      421 cgtcggtgga ctcctggatg atcggatcgg ggacgttatc caccctgacg tcttccttgc
      481 catgccagac tacggccttc acggctccct cccccctacc ccggtgagca tctcgcgtat
      541 tcggaagcgt ccgcccgtct tcggcgagag cggtggggtg tcgatgcccg agccggtccg
      601 caatccttcg aggaggtcga gcaaggcctc atcggcagtg tgctgcgggg tccagccgag
      661 ctcggtacgg gccctcgtgg tatccatgat ggggacgttc cgcgagaggt ccagccaccc
      721 tggcgggact ggctgcaacc gcagtgccca ggagagccca gcaccggcgc ggacgagttg
      781 ggcggatatc ggcacggggc gggcgtttag tatccgtccg atctcattgg cgtcgagtac
      841 gggctcagca gccaggttga acgggccgcg ggcatcctcg ttcactatgg cgagccggaa
      901 agcctcgccc acatcgtagg agtggaccac ctgggagcgc aggttctcta tgtcggggac
      961 gacgttgatc agggccgggt tggcgagcac gctggggaag aagggccctg cgaagagacg
     1021 ccttatcccc actgcagcct ccctcttgaa gacgagcccg gggcgcatcc tcacaagcct
     1081 cacgtcgggc gtctcagcct cgaaacggtc gagcctgcgt tcgacctcgg ccttatggcg
     1141 gccgtagtaa gagctcggga cgccgccggt cggccagctc tcgtccacgc ggcggtcctt
     1201 gggcccggcc gagtacgcgc cgaccgacga ggcgtagacg agcgagggaa caccagcctc
     1261 ctttgcggcc ctggcgaccc gcgtactccc ctcgacgttc accatccact gcttcgtgag
     1321 atcccgggag ggctggatga gccaggcgag gagtaccaca gcgtccgcgc ctgtaaagtg
     1381 agggacaaga tcgtcatcga caacatccgc cgaggcccac tctacctttg gcattgaaag
     1441 accaggcagc cggcgcgcca atcccaggac ggactctacg ccctcttctc tttcgagcga
     1501 ccggagcacg ctagtcccga tgttgccggt cgcccctacc accacgattc tcatgcaggg
     1561 cctttaccct tccacccgaa gggtgaaacg ccaaaagcgg gatgttgtac catcggccgg
     1621 gagcgagacc gggaggttag atgcgagtac tggtaacggg ggcgcggggc aaagtcggaa
     1681 gggctacggt ggccgcattc caggacgctg ggcacgacgt gcgggcgacc gacctgacgc
     1741 cgccggtctt cgagcggccc gaggaaggcg aggcggactg actcacttca gagagcaccc
     1801 gtctaccccc agatctgctc cgcggtctcg actatggtac ggagcttgta ctcctcgtct
     1861 tcgacgctta tggcgttgcc tataatggag gtggcgaagc cacactgcgg gctcaaggcc
     1921 agccgctcca aagggatgaa cctcgatgcc tcacggagac gggcctcgag ctcttgtttc
     1981 gtctcgcgcc ggggcgtctt ggtggtgacg agaccaagga cggcgacttt gtcccctggt
     2041 acgtgtgaga ggggtccgaa gtccccagag cgttcgtcgt cgtactcgag catcaacctc
     2101 tgcgcgttta tgccgccgaa gatgcggagt gccagcggct cgtacgagcc tgagaccagc
     2161 cagcggcttc actggttccc ccggcagagg tggaaggaga acgtgacgtc tgggtggtcc
     2221 ccgataacat agttgtcgag ctcgatcccc cgactcagcc agcggtccag atcccagccc
     2281 aacccct
//
LOCUS       DATMJA010000072         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_365875, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000072 DATMJA010000000
VERSION     DATMJA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365875"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="VNT12_01675"
     CDS             complement(<1..1624)
                     /locus_tag="VNT12_01675"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWI44850.1"
                     /translation="MSSAQIPKTYDHRAVEGRWYTEWERRGVFEADPNPHKVPYCIVV
                     PPSNVTGALHMGHALNGAIQDTLIRRARMKGYETLWLPGVDHASIAVQNVVERKVIRE
                     EGKSRWDLGREAFVERCREFALITREQILGQYKRLGASLDWRRLRYTMDDDYVDAVLT
                     AFIRLYNEGLIYRGNRITNWCPHDRSAISDLEVNYEEVEGNLYGIRYPFVDDKGPGPD
                     GEDYAQVFSTRPETMLGDVALVVNPEDARYSDLIGRRVMVPFVEREIEVFADEHVDPE
                     FGTGVLKVTPAHDPNDFEIGSRLGLDPVNVMNPDGTINAAGGPFEGMSREEAREAVVK
                     ELDGLGLLGGVRPYAFRVGHCDRCGAVIEPWLSEQWWCSMKPLAEPAIEALKNREISV
                     YPDSWRRETIRWLENIRDWNVSRQLWWGQRIPVWYGPDGEVAASKESPGPGFEQDPDV
                     FDTWFSSSLWPIATLGWPDEDAEDLRYFYPTDLLSTAREIMYLWVARMIMMGLHFEAE
                     IPFRKVNVHSIVLAEDGTKMSRSKGNTIDPLEL"
     gene            complement(1632..2891)
                     /gene="clpX"
                     /locus_tag="VNT12_01680"
     CDS             complement(1632..2891)
                     /gene="clpX"
                     /locus_tag="VNT12_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWI44851.1"
                     /translation="MRDPSDQLQCSFCGKSQRQVRKLIAGPGVYICDECIELCNEIID
                     EEFSGPEVLKDDDLPKPREINRILNEYVIGQEDAKRVLAVAVYNHYKRIQMGAEATDG
                     TELQKSNILLVGPTGSGKTLLAQTLAKILNVPFAIADATALTEAGYVGEDVENILLKL
                     IQAADFDVKKAETGIIYIDEIDKVARKADNPSITRDVSGEGVQQALLKILEGTVASVP
                     PQGGRKHPHQDFLQIDTKNVLFICGGAFGGLEEIIRQRIGKKSIGFGSDIDSRLKQED
                     DSILQFALPEDLLKYGLIPEFVGRLPVISTLRSLEEADLVRILTEPKNALVRQYRQFF
                     RYDKVDLTFTDDALGAIAELALERGTGARGLRSILESALLPTMYELPSRSDVNKCVVD
                     VDTVKEGVQPSLVTGSQKYSYDEEETA"
     gene            complement(2939..>3046)
                     /locus_tag="VNT12_01685"
     CDS             complement(2939..>3046)
                     /locus_tag="VNT12_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWI44852.1"
                     /translation="GQDYERIERDTDRDYIMGPEEGIEYGVIDNIVRNH"
BASE COUNT          522 a          927 c          935 g          662 t
ORIGIN      
        1 atagctccag tggatctatg gtgtttccct tggagcggct catcttcgtc ccgtcctcag
       61 cgagcacgat agagtggacg ttcaccttcc ggaaggggat ctcggcctcg aaatgcaggc
      121 ccatcatgat catacgcgcc acccagaggt acatgatctc acgcgccgtc gagaggaggt
      181 ctgtcgggta gaaataccgc aggtcttccg cgtcttcgtc aggccaaccc agcgtcgcga
      241 tgggccacag gctgctagag aaccaggtgt cgaacacgtc ggggtcctgt tcgaaaccgg
      301 gacccggcga ctctttggag gccgctacct caccgtcagg gccgtaccac accggtatgc
      361 gctgccccca ccagagttgc ctggagacgt tccagtcgcg gatgttctcc agccaacgga
      421 tagtctcccg ccgccaggag tcggggtaga ccgagatctc acggtttttc agagcctcga
      481 tggctggttc ggcgagcggc ttcatggagc accaccactg ttctgagagc cacggctcga
      541 tgaccgctcc gcacctgtcg caatgcccga ccctgaaggc ataagggcgg acgccaccga
      601 ggagccccaa tccgtccagc tccttcacga ccgcctcgcg ggcctcctcg cggctcatgc
      661 cctcgaaggg gccaccagcg gcgttgatcg tcccgtcagg gttcatgacg ttgacagggt
      721 cgaggccgag tctgctccct atctcgaagt cgttggggtc atgcgccggg gtgaccttga
      781 ggacacctgt accgaattcg ggatctacgt gctcgtcggc gaagacctct atctcccgct
      841 cgacaaacgg caccatgacc cggcgcccga taaggtcact gtacctcgca tcctcggggt
      901 tgacgacgag cgcaacgtcg ccgagcatgg tttccggacg cgtggagaag acctgggcgt
      961 agtcttctcc gtcgggacct gggcccttgt cgtccacgaa ggggtagcgg atgccgtaga
     1021 ggttgccctc cacttcctcg tagttgactt cgaggtccga gatagccgag cggtcgtgcg
     1081 ggcaccagtt ggtgatacgg ttgccgcggt agatcaaacc ttcgttgtac aagcggatga
     1141 atgctgttag caccgcgtcg acgtaatcat cgtccatggt gtagcggagg cgacgccagt
     1201 cgagggaggc gccgagccgc ttgtattgtc cgagtatctg ctccctcgta ataagggcga
     1261 actcgcggca gcgttcgacg aatgcctcgc gcccgaggtc ccaccgcgac ttgccctctt
     1321 cgcgtatgac tttgcgctcg acgacgttct ggacggctat cgaggcgtgg tcgacgcctg
     1381 gaagccacag tgtctcgtag cctttcatcc tggcacgcct gatgagggtg tcctgtatgg
     1441 caccgtttag cgcgtggccc atatgcaaag cgcccgtaac gttcgaaggc ggtacgacta
     1501 tgcaataggg cactttgtgg gggttcgggt ctgcttcgaa caccccgcgg cgctcccact
     1561 ctgtgtacca gcggccctcg acggcgcggt ggtcgtaggt ttttggtatc tgtgcgctac
     1621 tcaaggagtt ttcaggcggt ctcttcttcg tcgtaggagt acttctggga gccggttacc
     1681 agtgacggct gaacaccctc tttcaccgta tcgacgtcga cgacgcactt gttcacatcc
     1741 gagcggctcg gaagctcgta catcgtgggc agaagggccg actccaggat gctcctcagg
     1801 ccgcgggccc ccgtcccccg ctcgagcgcg agctccgcta tggcgccgag ggcatcgtcc
     1861 gtgaaggtaa ggtccacctt gtcgtagcgg aagaactgcc tgtactgcct caccagggcg
     1921 ttcttaggct cggtgaggat gcgtacgagg tcggcctcct ccaggctgcg cagggtggag
     1981 atcacgggca gacgtcccac gaactcgggg atcagcccgt atttcaaaag gtcctcaggc
     2041 aaggcgaact gcaggatgga gtcgtcttct tgcttgaggc gcgagtctat gtcgctgccg
     2101 aagcctatgc tcttcttgcc gatgcgctgg cggatgatct cctcaagacc gccgaatgcg
     2161 ccgccgcaga tgaaaaggac gttcttggtg tctatctgga ggaaatcctg gtgggggtgc
     2221 ttgcgccccc cctgcggcgg aaccgaggcg accgtgccct cgaggatttt gaggagtgcc
     2281 tgttgcacgc cttcgcctga gacgtcgcgc gtgatcgaag ggttatccgc tttgcgggct
     2341 accttgtcga tctcgtcgat gtagatgatg ccggtctcgg ccttcttcac gtcgaagtcg
     2401 gctgcctgga tgagcttgag gaggatgttc tctacgtcct cgcctacgta gcctgcttcg
     2461 gtcagggccg ttgcgtcggc tatggcgaag gggacgttga ggattttggc gagcgtctgg
     2521 gcgaggagag tcttgccgga gccggtcgga cccacaagca agatattgga cttctggagt
     2581 tccgtgccgt ctgtggcttc ggcgcccatc tggatgcgct tgtagtggtt gtagacggcg
     2641 acggccagaa ccctctttgc gtcctcctgg ccgatcacgt attcgttgag gatgcggttg
     2701 atctcccgcg gcttcgggag gtcgtcgtct ttgaggacct cgggccccga gaattcttcg
     2761 tcgatgatct cgttgcacag ctcgatgcac tcgtcacaga tgtagacgcc agggcccgcg
     2821 atgagcttcc tgacctggcg ctggctcttt ccgcagaagg agcactgcaa ctgatccgac
     2881 ggatccctca tgcctctaca tcctccccga catctctacc caatggcccc aggaacctct
     2941 aatggttcct aacgatattg tcgataacgc cgtactcgat cccctcttcg ggacccatta
     3001 tgtagtcgcg gtcggtgtcg cgctcgatcc tctcgtaatc ctgccc
//
LOCUS       DATMJA010000073         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_69482, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000073 DATMJA010000000
VERSION     DATMJA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69482"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..590)
                     /locus_tag="VNT12_01690"
     CDS             complement(231..590)
                     /locus_tag="VNT12_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HWI44853.1"
                     /translation="MTDLCALAGEDFCYLTTTGRVSGRPHEIEIWFALVPERRALYML
                     SGGGDRSDWVKNLSRDPDVEVRIAGEHYSGRAREAKDAEEDKLARRLLVEKYESSPGR
                     LESWRRRALPVVVDLAQ"
     gene            complement(694..2286)
                     /locus_tag="VNT12_01695"
     CDS             complement(694..2286)
                     /locus_tag="VNT12_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI44854.1"
                     /translation="MANIGDYEKTYADFDWETPERFNFGRDVVDRWAAQDRAAMVWLG
                     TSGEERRLDFAQFSVLSNRFANAARALGVGRGDRVMVLLGKVPEWHAILTGLLKLGAI
                     AIPCAPQLRAGDLKFRAEHSGSVAMISGPEGIEEVEKMREDVPGLEHFISLGGERAGW
                     ESYETLMEGASEDFTAEDTTADEGAFILYTSGTTKHPKGALHTHGYTHAKWMEARYWL
                     DLREGDRLWCTSGTGWAKSIWNVFLGPWSQGTEIFFHEGGFDPAERLELMELYDISVL
                     CQAPTEYRLLAKAPELERANLASIRHAVSAGEPLNPPVIERWKELHGLTIYDGYGQTE
                     NTLLVGNYPGLDLRPGSMGKPSPGCDVKIVDPEGNECQPEEPGDIALFGRIPAMFEEY
                     WEQPDATEAVFRNGYYLTGDRAYRDADGYLWFVGRSDDVILSAGYRIGPFEVESILIE
                     HPAVVESAVVPLPDEDRGSVVKAFVVVGEDYQPSEDLAKEIQDYCKENTAPYKYPRSI
                     EFIDDLPKTTSGKIRRVELREE"
     gene            complement(2377..>3096)
                     /locus_tag="VNT12_01700"
     CDS             complement(2377..>3096)
                     /locus_tag="VNT12_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWI44855.1"
                     /translation="ALASAGLDEFYGRNLPAPPASFPPQRFVEVSQLYGRHAVAVNAR
                     GERYADEGADWSETALTQATAHQPALYAWYVLDAGGLEGRVRERTANEMVETAKSTGG
                     TVIEAASLKELADRLAERGVPRETFLRTLEEYNAAVAVGGDPSPPRSGQAWPLRVPPF
                     VAVKVAPSITHTVGGLAVDAGCRVLRRTDGRPIPGLYAAGVEVGGVSVGGYTSGLASA
                     LVFGGTAAESAIADIQELDDP"
BASE COUNT          510 a         1034 c          926 g          626 t
ORIGIN      
        1 gtgagtcccg tcgaaccagg cctgtatctc ccgtgccgct cgcgggctgg tctcgaacgt
       61 cgcgatacgc tcctcgatgc cctcgttgta gatgcgggct atggctttcg tgtcttccga
      121 actggctgct ctggcccaca cgaccgtatc ctcacctcga ttcttccaga taacgccaac
      181 gcgccctcct gccgcagtcc tcactctttg gcgccacccc cgcttagccc ctactgcgcg
      241 aggtccacca ccacgggcag tgctctgcgg cgccagctct ccaggcgacc cggactgctc
      301 tcgtacttct cgaccagcaa tcgccgcgcc agcttgtcct cttccgcgtc tttcgcctcg
      361 cgggcgcgac cgctataatg ctctcccgct atgcggacct ccacatcagg atcgcggctg
      421 aggttcttca cccagtccga gcggtcgcct cctcccgaga gcatgtagag agcgcgtctt
      481 tccggcacga gggcgaacca gatctcgatc tcgtgcggcc tgccggatac cctccccgtc
      541 gtcgtcaagt agcagaagtc ctcgcccgcg agcgcacaca ggtcagtcac aggcatcagg
      601 tttcaagcta gaagcttcag gtttgcatgg aaggcagcgt agcaagaaac ctttccctgg
      661 cacctgatac ctgtagcctt cattttatcg gtttcattcc tctcgcagtt cgacgcgacg
      721 aatcttgccg ctcgtcgtct tgggtagatc gtcgatgaac tctatactgc gcgggtactt
      781 gtacggggct gtgttctcct tgcagtagtc ttggatctcc ttcgccagat cctctgaggg
      841 ctggtagtct tcgccaacga ctacaaacgc cttgacgacg cttcccctgt cttcatcggg
      901 cagaggtacg acggcgctct cgacaactgc gggatgctcg atgaggatgc tctcgacctc
      961 gaagggcccg atgcggtaac cggcagagag gatcacgtcg tccgaacggc ccacgaacca
     1021 gaggtagccg tccgcatccc tgtaagccct gtcgcctgtc aggtagtagc cgttacgaaa
     1081 aaccgcctcg gtcgcgtcag gctgctccca gtactcttcg aacatcgcgg gtatacgccc
     1141 gaagagggcg atgtcgccag gctcctcagg ctggcactcg ttaccttcgg ggtcgacgat
     1201 cttcacgtcg cagccaggag atggtttgcc catcgagccc ggtcgtaagt cgaggccggg
     1261 atagttgccg acgagcaggg tgttctcggt ctgcccgtaa ccgtcgtaga tggtcaggcc
     1321 gtgaagctct ttccaccgct cgatgacggg cgggttcagc ggttctccgg cggagacggc
     1381 gtggcggatg gaggcgaggt tcgcgcgttc cagttccgga gccttggcga ggagccggta
     1441 ctcggtcggc gcctggcaga ggaccgaaat gtcatagagt tccatcagct ccaggcgttc
     1501 ggcagggtcg aaaccgccct cgtggaagaa gatctccgtc ccctggctcc acggtcccag
     1561 aaacacgttc cagatgctct tggcccagcc tgttcccgag gtgcaccaca gcctgtcgcc
     1621 ctccctgagg tcgagccagt acctggcctc catccacttg gcatgcgtgt agccgtgggt
     1681 gtgcagggcg cctttgggat gcttcgtcgt ccccgaggtg tagaggatga atgcgccttc
     1741 gtctgcagtc gtgtcctcag ccgtaaagtc ctcagaggcc ccttccatca acgtctcgta
     1801 ggattcccat ccggcgcgct ctccgcccag agagatgaag tgttctagac ctgggacgtc
     1861 ttcgcgcatc ttctcgacct cttcgatgcc ttcagggccc gagatcatgg ccacagagcc
     1921 agagtgctcg gccctgaact tcaggtcgcc ggccctgagc tgcggggcgc atgggatggc
     1981 aatagccccc agcttcaaga ggcctgtcag gatcgcgtgc cactcaggca ccttgccgag
     2041 taggaccatc accctgtcgc cgcgtccaac gcccaacgct ctcgccgcgt tggcgaacct
     2101 gttcgagagg acggagaact gggcgaagtc gaggcggcgt tcctcgccgc tcgttccgag
     2161 ccagaccatg gccgcccggt cctgcgcggc ccatctgtca accacgtcac gcccgaagtt
     2221 gaacctctcc ggcgtctccc agtcgaagtc tgcgtaggtt ttctcgtaat cccctatatt
     2281 cgccaaggtt cctcaccttt ccccaaaggt cgctttccgg aatcgcgttc tactagaacg
     2341 agtatctctg gcacgtgcag aggagcactc gacgggctag ggatcgtcta gctcctgaat
     2401 atctgcgatc gctgactccg cggcggtgcc cccgaagacg agggccgagg cgaggccgct
     2461 ggtgtacccg ccgacggaga ctcctcctac ctcgaccccc gcggcgtaga ggcccgggat
     2521 tggccgtccg tccgtcctgc gcagcaccct acagcccgcg tccacggcga gcccgcctac
     2581 cgtgtgcgtt atggatggcg cgacctttac agccacaaag ggaggaacac gcaagggcca
     2641 tgcctgcccg ctcctcgggg gggagggatc tccacccacg gcgacggcag cgttgtactc
     2701 ctccagggta cggaggaagg tttcgcgggg cacgccacgt tcagcgagcc tgtccgcgag
     2761 ctccttgagg ctcgcagcct cgattactgt tccgcccgtg ctctttgccg tctcgaccat
     2821 ctcatttgcg gtccgctccc gcacgcggcc ttcgagccct ccggcgtcga ggacatacca
     2881 ggcatagagt gctggctggt gtgccgtggc ctgtgtcagg gccgtctcgg accagtccgc
     2941 gccctcgtcg gcgtagcgtt cgccgcgggc gttgaccgct acggcgtggc ggccgtagag
     3001 ctgggagacc tcgacgaacc gttggggggg aaaactggca ggaggagccg gcaggttgcg
     3061 gccgtagaac tcgtcgaggc ctgcgctcgc gagggc
//
LOCUS       DATMJA010000074         4648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_129024, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000074 DATMJA010000000
VERSION     DATMJA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129024"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VNT12_01705"
     CDS             complement(<1..620)
                     /locus_tag="VNT12_01705"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI44856.1"
                     /translation="MRESTAPDPGDTRRAGRLSPDFWKFLVGQTVSSLGSSFTVFALP
                     LLVFKLTGSALSLAYVTAAEYLPYLLFGLIIGAGVDRVDRRRLMIFVDTVQALVIFSI
                     PLLAMLDLLSLWWVYAVGFVSSTLWVCFNTAEFAALPSLVSKDDLVAANGRLQASYSA
                     ATVAGPLLAGLLMALVPVYTVLVLDAFSFLVSVLCLTLIRSSFNAD"
     gene            complement(760..2625)
                     /locus_tag="VNT12_01710"
     CDS             complement(760..2625)
                     /locus_tag="VNT12_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI44857.1"
                     /translation="MLKINTRDSEVRRKGRILAVFILGILGSGIGLAGFNLVQGSHKY
                     YISNGVFLSLIVGLFMLNRLGYVNIAAILTTTTMTVGPVVFLSQQDLAETYIVMCVPV
                     FVASFLIVPWAGLAVGAIVVLGTLVLGNASITYLSLFVFATVSVIVYFLANTVDSAYR
                     ENRYRALHDSLTDLPNRSLFTNRLVEAVERSSKQRVSCGVLFIDLDNFKVINDSLGHT
                     AGDELLVVIARRLLSCLRPADTPARLGGDEFAVVLDGIADIDDVIKISDRMHKVVQAP
                     VQLGVRQVYISTSIGIALGKANVADPTTLLRNADVALYEAKKEGKGRTKVFDWSMYSR
                     ARKRLELESELRLAIEQEKLSLYYQPMVCLSTGKVACMEALVRWEHPERGLIYPEEFI
                     PLAEETDLILPLGYWVMQSACHQAREWTRLGSDGPPLVLSVNVSVKQFQHPDFVGAVE
                     KILRDTGLEPSRLQLEITETVVTENLEYAAGLLKDLKRLGVQLAIDDFGQGNSSLGAL
                     RTFPLDNLKIDKSFVAGLGKNAQDTSIVQLVMDLAHTLDMEATAEGVESAEQLILLEQ
                     MGCDLAQGYYFAKPLAVEAAADLFADSPQWLVRRRDPLDRWQPGSFLDARHLPES"
     gene            complement(2802..4337)
                     /locus_tag="VNT12_01715"
     CDS             complement(2802..4337)
                     /locus_tag="VNT12_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HWI44858.1"
                     /translation="MVIKGYAHAVRLSRYLPFAVSELLRTDLSLLTQPTDYDSSWAAY
                     LVEENGSPSYPDVLPTLLERQHPDGGWGSRVSHSHDRLLTTLAVVLLLARFGNRRRDK
                     ERQAVGEHYIWRVARELDHDGQATVGFELLLPALLERGKRMGSNLPYAHLRRYETARA
                     KKLNLLPSERIFQSTTTALFSLEAFVDDMDLNDATSLLQQDGSMASSPSATAALLGQT
                     ADWRVRFPRSTGYLEDLLGRYDGGLPAMAPCGIFARAWVLHYLQHGRLLSGYESLLRP
                     HYRYLREHLAAGGVGFSPHVFPDADDTAVTLFVLHNAGYDVDGSCLLQYESDDHFAVY
                     PGELDPSISANLHILEALETLPAVVRQRTREKVIGYVLGARQPGGYWEDKWHASVYYP
                     TSQAVMALLPHAPGRMEVTLDWLLSTQHTDGSWGQYGPTVEETALSLLALLSYYRANR
                     CVAQERLRLAARYLIANEMPFKDDYPELWIAKALYAPTFVIRSIALAALGLYLDTFGD
                     KGL"
BASE COUNT          943 a         1443 c         1419 g          843 t
ORIGIN      
        1 ctgtcggcgt tgaaactcga cctgatcagc gtcaagcaca ggacagagac caggaatgag
       61 aaggcgtcga ggaccagcac cgtatacacc ggcaccagag ccatcagcag gcccgccagc
      121 aacgggcccg ccaccgtcgc cgcagagtag cttgcctgga ggcgcccgtt ggccgccacg
      181 aggtcatcct tgctgaccag gcttggcaag gccgcgaact cggcggtgtt gaaacagacc
      241 cacagcgtcg agctgacgaa ccccaccgcg tacacccacc acaaagacag cagatccagc
      301 atggccagca atgggataga gaagatgacg agggcctgaa ccgtgtcgac gaagatcata
      361 agccgtctgc gatccacccg gtcgacgccc gcgcctatga tgagaccaaa taacagatat
      421 ggcaagtatt cagcggcggt gacgtacgcc aggctcaagg ccgagccagt aagcttgaag
      481 accagcaacg gtagggcgaa cacggtgaag gagctaccga ggctcgaaac cgtctgcccc
      541 acgaggaact tccagaaatc cggggagagt ctgcccgcgc ggcgagtatc tcccggatca
      601 ggtgccgtgg actcccgcat agccgacctt taggctgtta caggatagat gctgtgctga
      661 ccggcggcga gcaggaagca ccagccaagc agtctgcgtg cgggttcacc tctgctcacg
      721 cgtgatcctc cttagagggc cgaaagtgta tggccaggat cacgattcgg gtaagtgcct
      781 ggcgtcgaga aacgagccgg gctgccaacg gtccagcgga tcacgccgac gaaccaacca
      841 ttgtggagag tcggcgaaca ggtccgctgc cgcctcgacc gcgagaggtt tcgcgaagta
      901 gtatccctgt gccaggtcgc atcccatctg ctcgagcagg atgagttgct cagcgctctc
      961 tactccctcc gcggtagcct ccatgtccag ggtgtgagcc aggtccataa ccagttgcac
     1021 gatagaggtg tcctgggcgt tcttccccaa tcccgctacg aacgacttgt cgatcttgag
     1081 gttatcgagc gggaacgtcc tgagtgcgcc aagcgaggaa ttgccctggc cgaagtcgtc
     1141 gatagccagc tgcacaccga gtcgcttcag atccttgagc agccccgccg catactccag
     1201 attctcggta accacggtct ccgtgatctc cagctggagg cgggacggct ccagacccgt
     1261 gtcccgcaag atcttctcga ccgcacccac gaagtctgga tgctggaact gcttcacgga
     1321 cacgttgacg ctgagaacca ggggagggcc gtccgagccg agccgcgtcc attcgcgtgc
     1381 ctggtgacag gcactctgca taacccaata tcccagaggt aagatcaggt ccgtctcctc
     1441 ggcgagtggt atgaactctt ccggatagat caagccgcgt tcgggatgct cccagcgcac
     1501 cagggcttcc atgcacgcta ccttgcccgt gctcagacac accatgggct ggtagtagag
     1561 actcaacttt tcctgttcta tggccagtcg aagctcgctc tccagttcca gccgcttcct
     1621 cgctcgggaa tacatgctcc agtcgaacac cttggtgcgc cctttgcctt ccttcttggc
     1681 ttcgtacagc gctacatccg cgttgcgcag gagggtggtc gggtcggcta cattggcttt
     1741 gccgagagcg ataccaatgc tcgtgctgat atagacctga cgaactccca actgcacggg
     1801 agcctgcacc actttgtgca tccggtcgga gattttgatc acgtcgtcga tatctgcgat
     1861 gccgtccaga acgacggcga actcgtcccc gccgagccgg gccggggtat cggcgggacg
     1921 cagacaggat agcaagcgcc gggcgatcac aaccagcaac tcgtcgccag ccgtatggcc
     1981 gagactgtcg tttatgacct tgaagttgtc gaggtctatg aacaacactc cacagctgac
     2041 gcgttgcttg gatgagcgct ctacggcttc cacgagccgg ttcgtgaaca gcgagcggtt
     2101 gggcaggtcg gtcaaggaat cgtggagtgc ccggtaacga ttctcccggt aggcgctgtc
     2161 gacggtgttg gccaggaagt atacgatcac gcttacggtc gcgaagacga acaacgagag
     2221 ataggtgatg gaagcgttgc ccaagaccag ggtgcccagc accactatgg cgcccaccgc
     2281 cagcccggcc cagggcacga tcaggaaact cgccacgaag acgggcacac acatgacgat
     2341 gtaggtctcg gccaggtcct gctgcgagag gaagacgacc ggaccaaccg tcatcgtcgt
     2401 cgtggtgagg atggcggcga tgtttacata cccgaggcgg tttagcatga acaggcctac
     2461 gatgagggac aggaatacac cgttggagat atagtacttg tgcgatccct gtaccaggtt
     2521 gaacccggct agaccgattc cgctcccaag gatcccgagg ataaagacgg cgaggatacg
     2581 ccccttgcgc cggacctcgg aatctcgggt gttgatcttg agcaagacta ccggaccatc
     2641 agatcagtaa agcctcgacc ttcgatcggc gagcctgtca ctctcgcaac cgcgcagaca
     2701 ctctgtcggc ccgcgcctgc tgctctcgag ccgggggttt gctccgacca tgcctggaca
     2761 tcgggtgccc ggcacgtcac ctttcttcgc ttcggtccca cctatagccc tttgtcgccg
     2821 aacgtgtcca gatacaggcc cagggcggcc aacgcgatgc tacggatcac gaaggtaggc
     2881 gcgtacaacg ctttcgcgat ccacaactcc gggtagtcat ccttgaacgg catctcgttc
     2941 gctatgaggt aacgggcggc gaggcgcaaa cgttcttgcg cgacgcagcg gttggcgcga
     3001 tagtagctca ggagggcgag caaggacagc gcggtttcct ctaccgtggg tccgtactga
     3061 ccccacgagc cgtccgtgtg ctgggtcgag aggagccaat ccagggtgac ctccatccgg
     3121 cccggcgcat gggggagcaa agccatcacg gcctggctcg tcggatagta caccgaagca
     3181 tgccacttgt cctcccaata accgccgggc tgacgcgcgc cgagcacgta gcctataacc
     3241 ttctcgcgag tccgctgccg cactacggct ggcagcgtct ccagggcctc gagaatatgg
     3301 aggttcgcgc ttattgacgg gtcgagctcg cccgggtaaa cagcgaagtg gtcgtcgctc
     3361 tcgtactgga gcaggcacga cccgtcgacg tcgtagccgg cgttatgcag cacgaagaga
     3421 gtcacggcgg tatcgtccgc gtcggggaac acgtgcgggg agaagccgac gcctccggcg
     3481 gccagatgct cgcgaaggta ccgataatgc gggcgcaaca ggctttcgta gccggagagc
     3541 aggcggccat gctgcaggta gtgaagcacc caagcccggg cgaatatccc gcatggagcc
     3601 atggccggca aacccccgtc gtaccgtccc aggaggtcct ccaggtaccc ggtgctgcgc
     3661 ggaaaacgaa cccgccagtc tgcggtttgg ccgagaagcg cggccgtcgc cgacggtgag
     3721 ctggccatcg acccatcctg ctgaagtagg cttgtcgcgt cgttcaggtc catgtcgtcc
     3781 acgaaggctt ccagcgagaa cagcgcggtg gtggtgctct gaaagattcg ctcgctcggt
     3841 agcaggttca gcttctttgc gcgcgcggtc tcgtagcgcc tcaggtgggc gtatgggagg
     3901 ttcgaaccca tcctcttgcc ccgctcaagg agggccggga gaagaagctc gaaccctacg
     3961 gtcgcctgcc catcgtggtc cagctcacgc gctactctcc agatgtagtg ttcgcccacc
     4021 gcctgccgct ccttgtcgcg cctccggttt ccaaagcggg ccagcaacaa cacaacggcc
     4081 agcgtggtca gcagacggtc atggctgtgg gatactcggc tgccccaccc gccgtcgggg
     4141 tgctggcgct ctagcagagt cggcagcacg tcagggtagg acgggctccc gttttcctcg
     4201 acaagatagg cggcccagct ggaatcgtaa tcggtaggct gcgtcagcag gctcaggtcc
     4261 gttcgcagta gctcgctcac ggcaaatggc aggtaacgag acaggcgcac tgcgtgggcg
     4321 tatcctttaa tgaccatgct cgacttcgac catcgtgacg cctcacctct tacatgggga
     4381 cgaggcataa gagacggtag acgccatttt gccccgtctg ccacggctcg accgcaaata
     4441 ctatagtgtt tccttcgcta gttctcaaca gtatccgcta ggaggtaagc agcgaatccg
     4501 gacgtacaac ccgcgccacc tccgttggcc gagtggctgg ggtggtcccg cgagcgggtg
     4561 gcgcgctgga tcttgtccag accgcacccg gtcgtgatgg gctggccctt caacggaacc
     4621 cgtcgctggt acctcctcca ccggggca
//
LOCUS       DATMJA010000075         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_69705, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000075 DATMJA010000000
VERSION     DATMJA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69705"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VNT12_01720"
     CDS             complement(<1..183)
                     /locus_tag="VNT12_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylate synthase"
                     /protein_id="HWI44859.1"
                     /translation="MNRDTPRANRLWAYLIVEELIRCGVGLFCVAPGSRSTPLVAALA
                     ANEKARSLIHFDERGTA"
     gene            complement(158..1585)
                     /locus_tag="VNT12_01725"
     CDS             complement(158..1585)
                     /locus_tag="VNT12_01725"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HWI44860.1"
                     /translation="MLSREYAGPRGENGAAPGARWLLADRVSHALSEARLESDADPVE
                     RIVRLAVPAGRIDPFRWLSEQRMFPKVYWSGREDRSGVAAVGIADLREVGVSEGPGSL
                     SKLLASLPDSGVSGARYYGGARFDPLGKPDEEWAAFCAYRFVLPRFELHAGKSETTLV
                     CNLVLPRDTDDASKIVQEIEDLSLPEGASGALLPAPVLREDSPDLQGWRENVERALAA
                     FSEGHLGKVVLARRAEFGFCRDLDPTLLLESLKAATPGCFHFYAEPESGTAFLGASPE
                     RLFRRDGCSVESEAVAGTRSRGISLAEDEGLRDDLLHSAKDLSEHTYVSKGIREALEP
                     LCEELEIEDCVSEMKLARRRHLRSKVRGTLRDGVTDAELLDAMHPTPAVGGHPRSEAE
                     EQIHALEPFDRGWYAGPVGWIGQEASEFAVGIRSGLVRGRTLALFSGAGIVAGSVPEK
                     EWAEIEQKIGDFTGMFGLEQGHAAS"
     gene            complement(1681..>2035)
                     /locus_tag="VNT12_01730"
     CDS             complement(1681..>2035)
                     /locus_tag="VNT12_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HWI44861.1"
                     /translation="GVAFALREALGALEDLGTIAPELRLAGGGTGGGSGEPWRQLLAD
                     VLGRPLWLLPDEVSSVASARGAAFLAGLASGIYPAAEDTLPLTPEPESHVRPRETAYE
                     AAYERYKELYPRLYR"
BASE COUNT          368 a          709 c          612 g          346 t
ORIGIN      
        1 ggcggtcccg cgctcgtcga agtggattag gctacgggcc ttctcgttcg cggcgagcgc
       61 cgcaaccagg ggcgtcgagc gcgagccagg ggcgacgcag aacaagccta cgccgcagcg
      121 gataagctct tcgacgatca ggtacgccca gaggcgatta gctcgcggcg tgtccctgtt
      181 caagcccgaa catccctgtg aagtcgccga tcttctgctc gatctcggcc cactccttct
      241 caggcacgga gccggcgacg atcccggccc ccgaaaagag ggcgagcgta cgtccccgca
      301 ccagccccga gcggatacca acggcgaact cggaggcttc ttgcccgatc caacctacag
      361 ggccagcgta ccacccacgg tcgaagggtt cgagggcgtg tatctgctcc tccgcctcgc
      421 ttcgcggatg cccgccgacg gctggcgtag ggtgcattgc gtcgagcagc tcggcgtccg
      481 tcacgccatc tctgagcgtc ccacggactt tcgacctaag gtgccttctc cgcgccagct
      541 tcatctcaga gacacaatcc tcgatctcca gctcttcaca gagcggctcc agcgcctccc
      601 ttataccttt gctcacgtaa gtgtgctcgg agaggtcttt agcgctgtgc aacaggtcgt
      661 cgcgcaaccc ttcgtcctcc gccaaagata ttccacggga ccttgtccct gccacagcct
      721 cgctctccac cgagcatccg tcacggcgga agaggcgttc gggagaggcc ccgagaaacg
      781 ccgtcccact ctcaggctcg gcgtagaaat ggaagcagcc aggcgtagcc gccttcaggc
      841 tctcgagtag cagcgtagga tccaggtccc tacagaaacc aaattcagcc ctgcgggcca
      901 gcacgacctt acccaggtgc ccttccgaaa acgccgcgag agcccgctcg acgttctccc
      961 tccaaccctg caggtcgggg ctatcctcac ggagtaccgg tgccggcaac agagcgccgg
     1021 acgcgccttc aggtaatgag aggtcttcga tctcctgcac tatcttcgag gcgtcgtccg
     1081 tatccctggg gagcaccagg ttgcagacca gcgtcgtctc cgacttcccc gcgtgcagct
     1141 cgaagcgtgg caggacgaac cggtaagcgc agaacgcagc ccattcttcg tcgggcttgc
     1201 cgagcggatc gaagcgcgct ccgccgtagt agcgtgcccc cgaaacccct gaatcgggca
     1261 gcgacgcgag cagcttggac agagatcccg gaccctcaga gacgcccacc tcacgcaggt
     1321 ccgcgatccc cacggccgcg accccggatc ggtcttcacg ccccgaccag tagaccttcg
     1381 ggaacatccg ctgctctgag agccacctga aagggtctat ccggcccgcg ggtacggcga
     1441 gacgcacgat ccgctcgacc gggtcagcgt cgctttccag ccttgcctct gagagcgcat
     1501 ggctcacccg gtcggccagc aaccagcggg ccccgggtgc cgctccgttc tccccacgag
     1561 ggccggcgta ttccctgctc agcaagatct atgccacctc atcatctcag ggtgtagtgt
     1621 agcaaggagt atctagcggt ttagcggctt ctctcacagt cgcaggccag tgtcaggcac
     1681 tcaacggtag agccttgggt agagttcctt gtaccgttcg taggcggcct cgtaggccgt
     1741 ctcacggggc cgtacgtggc tctcgggttc gggggtgagg gggagcgtat cctcggccgc
     1801 agggtagata ccggaagcga ggccggccag gaaagcagcg ccacgggctg atgccacgga
     1861 ggatacctca tcgggcaaca gccacaacgg gcggccaagc acatcggcca gtagttgccg
     1921 ccagggctcg cctgagcctc ctcctgttcc acctccggcg aggcgcagtt cgggggcgat
     1981 ggtccccaga tcctccagtg ctccgagtgc ctcacgcagg gcgaacgcga cgccc
//
LOCUS       DATMJA010000076         4782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_366319, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000076 DATMJA010000000
VERSION     DATMJA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366319"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VNT12_01735"
     CDS             <1..398
                     /locus_tag="VNT12_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI44862.1"
                     /translation="LDIIEEVRDEGRTVFFSSHNLAEVERLCDRVGIIRGGRLAAVET
                     TETLIDKSFRHVVLTFAEDVDPKPFEALSGLRNLKSDGKRIYFTLYDDLDEMVKLAAR
                     HRLVNMEYERPSLEEVFLTYYERENGEVR"
     gene            395..1240
                     /locus_tag="VNT12_01740"
     CDS             395..1240
                     /locus_tag="VNT12_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWI44863.1"
                     /translation="MSVVLNRGEKGRHTARRGWTLFALVSHTVRMQLRGVIIWGAALG
                     LYSAAMVASFTAIEGSADQLNQLIEAYPKGMLEAFGVTDLGDPANYLHSQVFGLAPLA
                     LSFFPILALANALAGAEERGTIDVLLGNPLPRWQLVAGTFVAVAVSLLGICAIVGLLT
                     WGTAVLTGVELSLREVAEAVLNLWPMCIMFGALALLCSAFFHRRSLAITIPSFLLFGM
                     YLIDTIGRASEDLEDWRPLSVFYYYGSAIENGIDWRHFGSITLVALALLLLATLVFRR
                     RDIYT"
     gene            1309..2601
                     /locus_tag="VNT12_01745"
     CDS             1309..2601
                     /locus_tag="VNT12_01745"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI44864.1"
                     /translation="MRGLLIRARGIATLRGVLDSAVAQDLLALLQLLGEERPDAGAVA
                     KVFGRLWEGLALDEERLLPDAWQSHLVGRILDDENPFSLGAEKGEVNSSVLEQAGRDL
                     RTLREMFALDAAVLLGRVESAVPALAGIWVPWTSREPVDDSPRRTIARKLSAAEDWGA
                     CAELLAGHYARHGAGPYGRHRAFLWREGRLLAVSRPDPVSLAELVAYEREREPLIRNT
                     ERFLAGLPAHHALLYGLPGTGKSSTVKAIMNEYADLGLRLVEVKKEDLAELQVVLEVL
                     GGRGRRFVLFVDDLSFEEHEVEYKALKALLEGSVEEPPENVRLYATSNRRNLIRETFS
                     ERGDGSAGDDVHARDTMQEKLSLAGRFGLRLTFPSPDQARYLEIVAGLARERGLAIIA
                     EDLRERALLWDRWHAGRSGRTARQFVDELEAELDEEQS"
     gene            complement(2637..3080)
                     /locus_tag="VNT12_01750"
     CDS             complement(2637..3080)
                     /locus_tag="VNT12_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI44865.1"
                     /translation="MSTKVILLVEDNPDDELLTLRALKKSGVQNEVVVAHDGVEALDY
                     VFASGPYSGRDPTVMPQLILLDLKLPKVDGLEVLKRLRSDERTRRLPVVILTSSREQQ
                     DMLDGYGLGANSYIRKPVDFEQFVRAVEQLRLYWLVLNEAPPGIM"
     gene            complement(3193..3642)
                     /locus_tag="VNT12_01755"
     CDS             complement(3193..3642)
                     /locus_tag="VNT12_01755"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWI44866.1"
                     /translation="MQQDPNLGSERERASGDQVGASTGTMSAQDERTWSIIAHLSVLL
                     LIIGLMPFGALLVWLLYRDRSRKVRFHALQALWYQIAWIVILVTYALLSTALSLVIIG
                     IFMFFLLPILALIPLIHGCYAAYKVSQGVEYRYPYIADRIEGPRGVV"
     gene            3922..>4782
                     /locus_tag="VNT12_01760"
     CDS             3922..>4782
                     /locus_tag="VNT12_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWI44867.1"
                     /translation="MLLRVLLVEDSEDDALLLVRMLRKGGYDPAWERVDTPAAMEAAL
                     DGGSWDLIISDHSMPAFSSLAALGLLRRKGFVDVPFIIVSGRIGEDAAVAAMKAGAHD
                     YLMKDNLARLNSAIERELRDADVRRERRQAEKKYRSIFENAVEGIFQTTADGRFLTAN
                     PAMARMLGYESPEELLGAISNIRDQLYVEPGRREEFYQRALRDGFVSGFEVQMHRKDG
                     NLVWVSANARAIYDAGGGISGYEGTAENITERKRAEEALRQIREAERRRIARELHDVV
                     LQDLTYALQSM"
BASE COUNT          891 a         1460 c         1527 g          904 t
ORIGIN      
        1 ttctcgacat catcgaggag gtcagggacg aggggcgaac cgtcttcttc tcctcgcaca
       61 accttgccga ggtcgagcgg ttgtgcgaca gggtggggat catccgcggc ggcaggctcg
      121 ctgctgtcga gacgacggag acgctcatag acaagtcttt cagacacgta gtccttacgt
      181 ttgcagaaga cgtcgatccg aagccattcg aggcgctctc aggcctcagg aacctgaaga
      241 gtgatgggaa gaggatctac ttcaccctct acgacgacct cgacgagatg gtcaagctgg
      301 cggccaggca caggctcgtg aacatggagt acgagcgtcc cagcctcgag gaggtcttcc
      361 tcacctacta cgagcgggag aacggagagg tgcgatgagc gtggttctga atcgaggcga
      421 gaagggaaga catacagcaa ggcgcgggtg gacgctgttc gcgctcgttt cgcacacggt
      481 caggatgcag ttgcggggtg tgatcatctg gggagcagcg ctcgggctct acagcgccgc
      541 aatggtcgcc tcgttcacgg ccatcgaggg gagcgcggac cagctgaacc aactgataga
      601 ggcctacccg aaggggatgc tcgaggcttt cggcgtaacc gacctgggcg accctgccaa
      661 ctacctccat tctcaggtct tcgggctcgc gccgctggcg ctgtctttct tcccgatact
      721 cgcgctcgcg aacgccctgg caggggccga ggagcgcggc accatagacg tccttctcgg
      781 caaccccctg ccccgctggc agctcgtcgc gggcaccttc gtcgccgtgg ccgtctcgct
      841 actcgggatc tgcgccatcg tcggcctgct gacgtggggc acggccgtcc tcacaggcgt
      901 tgaactctcc ctgcgagagg tggctgaggc cgtcctcaac ctgtggccca tgtgcatcat
      961 gttcggcgcc ctcgcgctgc tgtgctcggc attcttccac cgcagaagtc tcgccataac
     1021 catcccctcc ttcctgctct tcggcatgta ccttatagac accataggaa gggcttcaga
     1081 agacctggaa gactggcggc ctctctctgt cttctactac tacggttcag ccatcgagaa
     1141 cggcatagac tggagacact ttggcagcat aaccctcgtc gcactcgcac tcctgctctt
     1201 ggccacgcta gtcttcaggc gacgagatat ttatacatag cacgccgccg tactgacaag
     1261 ctgaggcgaa gccggagcgt gctaacatgc gttatggggt gcggggacat gcggggactt
     1321 ctgatcaggg cgcgagggat cgcgacgttg cggggcgtct tggactcggc cgtcgcgcaa
     1381 gatctcctgg cgcttctcca gctgctgggg gaggagcgtc ctgacgctgg cgctgttgcg
     1441 aaggtcttcg gacggttatg ggaagggctt gccctcgacg aggagcgtct gctccctgac
     1501 gcctggcagt cacatctcgt gggcaggatc ctcgacgatg agaacccgtt cagcctcggg
     1561 gctgagaagg gggaagtcaa ttcgtccgtg ctggagcagg cggggcggga tctgcgcacg
     1621 ctcagggaga tgttcgcgct tgacgcggct gtcttgctcg gcagggtcga gtcagccgtg
     1681 cctgccctgg ccggcatctg ggtcccctgg acgagtcgcg agcccgtgga cgactctccg
     1741 cggcgcacta tcgcgcgcaa gctctcggcg gcggaggact ggggagcgtg cgcagagctt
     1801 ctggccggcc actacgccag gcacggagcc ggaccctacg gccgccacag agccttcctc
     1861 tggagagaag gaagactttt ggccgtctct cgtcctgacc ctgtgagcct cgccgaactc
     1921 gtcgcctacg agcgcgagcg ggagcctttg atcaggaaca cagagcgttt tctcgccggg
     1981 ctgccggccc accacgcgct cctgtacggc ctgccgggta cgggcaagtc ctccaccgtc
     2041 aaggctatca tgaacgagta cgcggacctg gggctgcggc tggtcgaggt gaagaaggaa
     2101 gacctcgcgg agctgcaggt ggtgctcgag gtccttggcg ggcgtggccg gcgtttcgtg
     2161 ctcttcgtcg acgacctctc cttcgaggag cacgaggtcg agtacaaggc tctcaaggcg
     2221 ctcctcgaag gcagcgtcga agagccgccc gagaacgtca ggctctacgc gacctccaac
     2281 cgcaggaacc tgatccggga gaccttctcc gaacgtggcg acggatccgc aggcgacgac
     2341 gtgcacgccc gcgacaccat gcaggagaag ctctcccttg cgggccgctt cggactgcgc
     2401 ctgaccttcc cctctcccga ccaggcacgc tacctggaga tcgtagccgg actcgccaga
     2461 gaacgcggcc tggccatcat tgcagaagac ctgcgggagc gcgccctcct gtgggaccgc
     2521 tggcacgccg gacgctcggg ccgcaccgcc cgtcagttcg tggacgagct cgaagccgaa
     2581 ctggacgagg aacagagcta gtcagcaagg cacccgcagg gcgccttgct gactagctac
     2641 attatccctg gcggggcctc gttcagtacc agccagtaga gcctgagttg ctcgacggcc
     2701 ctgacgaact gctcgaaatc taccggcttg cggatgtagc tgttggcgcc gaggccgtag
     2761 ccgtcgagca tgtcctgctg ctctctggac gaagtcagga tgaccacggg caagcgccgc
     2821 gtacgctcgt ctgaccgcag gcgcttgagt acctcgagcc cgtccacttt aggcagcttg
     2881 agatctagca ggatcaactg cggcatgacg gtgggatccc tccccgaata ggggccgctt
     2941 gcgaaaacgt aatcgagggc ctcgactcca tcgtgggcta ccacgacctc gttctggaca
     3001 ccggacttct tcaacgcccg cagggtgagc agctcgtcgt ctgggttgtc ctcgaccagg
     3061 aggatgacct tggtactcat ctcgccccga gcccatcccc gaagggcctg cgcgtcctgg
     3121 ttctctcgcg tactctggtg tatctgcacg gcatctcgct ctgccctcag gccttgctag
     3181 gcggggaacg cctcagacta cgccccgcgg gccttctatc ctgtccgcga tgtacgggta
     3241 tcggtactct acgccctggc tcaccttgta ggctgcgtag caaccgtgaa tcagaggtat
     3301 cagcgccagg atgggcaaca agaagaacat gaagatgccg ataataacga gactcaatgc
     3361 ggtgctgaga agggcgtaag tcacgaggat cacgatccag gctatctggt accagagcgc
     3421 ctgcagcgca tggaagcgca ccttccggga acggtccctg tatagcagcc agacgagcaa
     3481 ggccccgaaa ggcatgaggc caataatcaa caagagtacg ctcaggtggg cgattatgga
     3541 ccacgttcgc tcatcctgcg cgctcatcgt acccgtcgag gctccgacct ggtctccgga
     3601 agctcgttcc cgctcactgc cgaggttggg gtcttgttgc atcttctctc cttccgtacc
     3661 agtcttggag tttgcatgta tccacgcccc gccatgatag ctctgcaagg gacaccgacg
     3721 catcccccaa atgagttatt tttggatgtt ttgcccactg gcggcgagtt gcccgaacgc
     3781 tcgggtaaga atagaataga gcttaatgtg atggggatta cacagattat cgtggcggag
     3841 gaagagcggt cggacttttg cctgctatac atgcgtacaa acatgggctg tggcctgcgc
     3901 ggagcttgtg cctgaatggg tatgctgctc agagtcttgc tcgtcgagga ttccgaggac
     3961 gacgccctgc tcctggtgag gatgttgcgc aagggtgggt acgaccccgc ctgggagcgg
     4021 gtcgacaccc cggcggctat ggaggccgcg ctcgatggag gcagttggga tctcatcatc
     4081 tccgaccatt ccatgccggc cttcagctcg ttggcagcgc tcgggttgct caggcgtaag
     4141 gggtttgtgg acgtgccttt catcatcgtg agcggccgga tcggggagga cgcagccgtc
     4201 gcggccatga aagccggtgc ccacgactac ctcatgaagg ataacctggc caggctcaac
     4261 agcgctatcg agcgggagct cagggacgct gacgtgcgtc gcgagcgccg gcaggccgag
     4321 aagaagtacc gttcgatctt cgagaacgcc gtcgaaggaa tcttccagac caccgccgac
     4381 ggacggtttc tgacagcgaa ccccgcgatg gccaggatgt tgggctacga gtctcccgag
     4441 gagctgctcg gggccatctc caatatcagg gaccagctct acgttgagcc gggacgcagg
     4501 gaggagttct accagcgcgc gctgcgcgat ggcttcgtga gcggcttcga ggtacagatg
     4561 caccgtaaag acggaaacct cgtgtgggtc tcggccaacg cccgcgccat ctatgacgcc
     4621 ggtggaggta tctcgggtta tgagggtact gcagagaaca ttacagagcg caagcgggcc
     4681 gaagaggccc tgcgccagat cagggaggcc gaacgtaggc ggatagcccg cgagttgcac
     4741 gacgtggtct tgcaggacct cacgtacgcc cttcagtcaa tg
//
LOCUS       DATMJA010000077         4513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_10811, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000077 DATMJA010000000
VERSION     DATMJA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10811"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VNT12_01765"
     CDS             complement(<1..1038)
                     /locus_tag="VNT12_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HWI44868.1"
                     /translation="MPTGREAGDSIPGDQDTIEGRTSTEVRDEAKSVEFTYRGIFQKT
                     LAANLCKHVVMAARKRDWLGSTIQRYSDSPERNGIPAKQFAVIAPDTETLEGHMAKYE
                     PNELDVITVLDDTMCDGIESWAWYGRQPINVRLRRGGTLLVVSNRTPEELLKVIPQKP
                     WDWTLAILPGTPSLGGLWVYRDDGTDMRVLGAIARTRSDIFEYGEIEAVVSESGLGEK
                     YLAALKNGHDEVETHEVKAGEGAEDDYEPVKLPGWREMREAIIVEAVKPGERNPHYKT
                     SSDRTMRPVVNFDTCIKCRQCWIDCPDECFDISDEELHPVNYSYCTGCGICANVCPVE
                     DCIVMVNDLEFV"
     gene            complement(1234..1659)
                     /locus_tag="VNT12_01770"
     CDS             complement(1234..1659)
                     /locus_tag="VNT12_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44869.1"
                     /translation="MDPSANPPPVPRFVFVAFYPEGPHAGPISIGLFGVQDGVLLPVF
                     SSREKAVDFAVRVLGAPPQKNENWDGVELPAEDLIQFLHTDASISYVAANPLPSGEVE
                     LIPTPEFVERLEQEAGDTDGPREDFRRSLVRSFVYGDVS"
     gene            complement(1682..1888)
                     /locus_tag="VNT12_01775"
     CDS             complement(1682..1888)
                     /locus_tag="VNT12_01775"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HWI44870.1"
                     /translation="MARTIRCTEAQRTLDFEVDRNCDFVARAESEDEALAELTDHMQT
                     VHNWNEIFEPDLEKVRVQNVHNDV"
     gene            2058..3245
                     /locus_tag="VNT12_01780"
     CDS             2058..3245
                     /locus_tag="VNT12_01780"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWI44871.1"
                     /translation="MKVNKWTRRHSNAEDGNAPPEAVPDSGDGGEIQQPPDGLSEAAI
                     REALRDVRDPEIGRDLISLNMVRGVKIEGGNVAVGIALTTAGCPLKHRITTDIRDRLK
                     MIEGIEEVEVDFGVMTDEDRHNLLTSLHGSPSELAPAFRDESKTRIIAVVSGKGGVGK
                     STVALNLAAALDRAGHSVEILDADVHGSSIPVMLGSLGKPNVVEGVIFPIESDQGLKF
                     ISMGNFVNEGQAIIWRAPIVNKALTQLMRDVYWDEPEFIIVDMPPGTGDVALTVAQMI
                     PKAEALVVTTPQADAARVAVKAGKMAVQAHLKVVGVVENMGYLECPDCGKELRIFGGD
                     GGDRVAGELGSEVLGRIPMVPDATGEPGRSLFEVDSTPARVFDEIAAALAASKVRRRI
                     KVL"
     gene            complement(3341..4270)
                     /gene="mdh"
                     /locus_tag="VNT12_01785"
     CDS             complement(3341..4270)
                     /gene="mdh"
                     /locus_tag="VNT12_01785"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWI44872.1"
                     /translation="MAKRKVTVVGAGNVGGTTAQRLAERNYADVVLVDIVEGLPQGKA
                     LDILESGPILGYDSNVTGANDYEETAGSDVVVITSGSPRKPGMSRDDLLKTNQNIVAS
                     VTEEVVRHSPETILIVVANPLDAMCHVAMETSDFPRERVVGMAGILDTARYRTFIAEE
                     LQVSVRDVFALVLGGHGDTMVPLPSMATVGGIAITELLPEDRVEAIVDRTRQGGAEIV
                     GLLQSGSAFYAPSAAAAEMVDAILLDQKRILPCAAYLQGEYGINDLFVGVPVKLGASG
                     VEDIIELGLTEEELGDLNDSANSVRDLVQVLHA"
BASE COUNT          842 a         1399 c         1366 g          906 t
ORIGIN      
        1 gacaaactcg aggtcgttga ccatcacgat gcaatcctcg accgggcaca cgttggcaca
       61 gatgccacac cccgtacagt agctgtagtt gaccggatgc agctcttcat cggagatatc
      121 gaagcactcg tcggggcagt cgatccagca ctgtcggcac ttgatgcacg tgtcgaagtt
      181 gacaaccggc cgcatggtcc tgtcagagga ggtcttatag tgcgggttgc gctcacccgg
      241 cttgacagcc tcgacgatga tggcctcgcg catctcgcgc caccccggca gcttgaccgg
      301 ttcgtagtcg tcctctgcgc cctcgcccgc cttgacctcg tgggtttcga cctcgtcgtg
      361 gccgttcttg agagcggcga gatacttctc gccgagtccg ctctcgctca ccacggcctc
      421 tatctcgccg tactcgaaga tgtcggagcg cgtgcgcgct atggcgccga gcacccgcat
      481 gtccgtgccg tcgtcgcggt agacccagag cccgcccagg ctcggcgtcc ccggcaggat
      541 ggccagcgtc caatcccagg gcttctgcgg gatgaccttc agaagctcct cgggcgtcct
      601 gttcgagact accagcagcg tcccgccgcg cctgagacgc acgttgatcg gctgccgccc
      661 gtaccaggcc caggactcga tcccgtcgca catcgtgtcg tctaagaccg tgatgacgtc
      721 cagctcgttg ggctcgtatt tggccatgtg gccttccaac gtctccgtat ccggcgcgat
      781 gaccgcgaac tgcttggccg ggatgccatt gcgctccggc gaatcactgt agcgctggat
      841 cgtcgatcct aaccagtccc gcttgcgggc cgccatcact acatgcttgc acaggttcgc
      901 cgctaacgtc ttctggaaga ttccccgata cgtgaactcg acgctcttgg cctcgtcgcg
      961 tacctccgta gaggtgcgcc cctcaatggt gtcttgatcc ccaggaatcg agtcgccagc
     1021 ttctctgcca gtcggcatcc tttctccttc cctagtaatc ggccctcaga gccaattcct
     1081 acacccctca gcttcagctg tcgaagctct ggcctgacca ctttaccaat ttgccaagca
     1141 tcctatcagc ggcaggtaac agggtcaatg ccatgttcca cagattgtat cagtatttcc
     1201 atgcgtcccg ttccgttatc gaggaacggg tagtcagctc acgtctccgt agacgaagga
     1261 ccggaccagg gaccggcgga agtcttctct gggaccatcg gtgtcccctg cctcctgctc
     1321 gaggcgctcc acgaactctg gggtggggat cagctccacc tccccggatg gcagcgggtt
     1381 tgcagcgacg taggagatgc tcgcgtcggt gtggaggaac tgtatgaggt cctcggcggg
     1441 gagctcgacc ccgtcccaat tctcgttctt ttgaggaggg gcgccaagta cgcggacggc
     1501 gaaatctacg gccttctctc gcgaggaaaa gacgggtagc aggacgccgt cctgaacgcc
     1561 gaagaggccg atgctaatcg ggcccgcgtg cggcccttcg gggtagaagg cgacgaacac
     1621 gaatctgggt acagggggcg ggtttgcgga aggatccatc gagccgcttc ggcgggagtt
     1681 gctagacgtc gttgtggacg ttttggacgc ggaccttctc gaggtccggc tcgaagatct
     1741 cgttccagtt gtgtaccgtt tgcatgtggt ccgtgagctc cgccaatgcc tcgtcctccg
     1801 actctgcgcg ggcaacgaag tcgcagttcc gatcgacctc gaaatccagt gtcctttggg
     1861 cttcggtgca ccgaatcgtc ctggccatct gcctctcctt tccctcttct tgatcatagc
     1921 agggtggtca aaaccgctca acggctccgc gactagatag aaattctcgt gataacatcc
     1981 ctcaaaaccg atgcctgaca cggcgttacg ccaacgccgc ttggcgacga acgcaaagca
     2041 gcgacggagg tcaacggatg aaagtaaaca agtggacccg gcggcactcc aacgccgaag
     2101 acggcaatgc gccgccggag gccgtccccg actcaggaga tggaggagag atccagcagc
     2161 ctccggatgg tctgtcggag gctgccattc gtgaggcttt gagggacgta agggaccctg
     2221 agatcgggcg cgacctcatt tccttgaaca tggtgcgcgg cgtaaagatc gagggtggaa
     2281 acgtcgccgt ggggatcgcg ctcacaaccg caggctgccc tttgaagcac aggatcacca
     2341 ctgacataag ggaccgcctc aagatgatcg aaggcataga agaagtcgag gtcgacttcg
     2401 gcgttatgac agacgaagac cgccacaacc tgctcacctc ccttcatggc agcccctcgg
     2461 agctggcccc cgccttccgc gacgagtcaa agacccgcat catcgccgtg gtgagcggca
     2521 agggcggggt aggcaagagc accgtggccc tcaacctggc agcggccctc gaccgggcag
     2581 gacattccgt tgagatactc gacgctgacg tgcatggctc ttccatccca gtaatgctcg
     2641 gctccttggg caagcccaac gtggtggagg gtgttatctt ccccatagag tctgatcaag
     2701 ggttgaagtt catctccatg ggcaacttcg tgaacgaggg gcaggccata atctggcggg
     2761 ccccgatagt gaataaggca ctaactcaac tgatgcgcga cgtctactgg gacgagccag
     2821 agttcatcat cgtggacatg ccgccaggca caggcgacgt agccctgacc gtggcgcaga
     2881 tgatccccaa ggctgaggcg ctggtggtca caacccctca ggctgacgcg gcccgcgttg
     2941 cggtcaaggc cggcaagatg gcggtgcagg cgcacctcaa ggtggtaggg gttgtagaga
     3001 acatgggcta cttagagtgt cctgattgtg gcaaggagct aaggatcttc gggggcgacg
     3061 gaggagatag ggttgcagga gagctaggct ctgaggtgct gggacgcatc ccgatggtgc
     3121 ctgatgccac gggtgagcca ggacgctccc tcttcgaggt agattcgacc cctgcgcggg
     3181 tgttcgatga gatcgctgct gcgctagctg caagcaaggt ccgcaggcgc ataaaggtgt
     3241 tgtagagccg ctcagcacgt cagcctgcgg agaccttacg gcctccgctt tcatccccac
     3301 aagatgtgga gacgcgaact attgtccggc ttgttgctgt ctatgcgtgc aacacctgca
     3361 cgagatctct caccgagttg gcgctatcgt tcagatcccc cagttcttct tcggtgagac
     3421 caagctcgat gatgtcttcg acgcccgagg cgccgagctt gacggggacg ccgacgaaga
     3481 ggtcgtttat tccgtactcg ccttgcaagt aggcggcgca ggggaggata cgcttctggt
     3541 cgagcaggat ggcgtcgacc atctcggctg cggctgcgga cggcgcgtag aaggcgctcc
     3601 cgctctggag gaggccgacg atctcggccc cgccctggcg ggtgcgatcg actatcgcct
     3661 cgaccctgtc ttcggggaga agctccgtga tcgcgatccc gcctactgtc gccatgctgg
     3721 gcagcggtac catcgtgtcc ccgtgcccgc cgaggacgag cgcgaagacg tcgcgcacgg
     3781 agacctgaag ctcttcggct atgaacgtgc ggtagcgggc cgtatcgagg atgccggcca
     3841 tgccgaccac ccgctcacga gggaagtcgc tcgtctccat cgccacgtgg cacatcgcgt
     3901 cgagcgggtt ggcgacgacg atgaggatcg tctccggcga atgccttacg acctcctccg
     3961 taacgctcgc cacgatgttc tggttcgtct tgagcagatc gtcacggctc attcccggct
     4021 tgcgggggct gccgctggtt atgacaacca cgtccgagcc tgcggtttcc tcgtaatcgt
     4081 tggcgcctgt tacgttggag tcgtatccga ggatagggcc actctccagg atgtctagcg
     4141 ctttgccctg gggaagtcct tccacgatgt ccaccagcac aacgtcggca taattgcgct
     4201 ctgccagcct ctgggccgta gttcctccga cgttacccgc gcctacgacg gtgaccttcc
     4261 tcttcgccat ctcgttccct cctgtgttcg actagccggg tgaggctatg atatgtgaat
     4321 acctcagagc ttgcctcgcc aaacccggat agaatcagag ccgtgagtgc gactttccag
     4381 gagcttcgtt ggatgtgatc catgccatac agacgcgcca gagcgtcggc cgcgtgaggc
     4441 aggaccccgt gcaaagagat ctcgtcgaac gcatcctgga gagcgccgtg cacgccccca
     4501 accacaagat cac
//
LOCUS       DATMJA010000078         4580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_543829, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000078 DATMJA010000000
VERSION     DATMJA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543829"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VNT12_01790"
     CDS             complement(<1..645)
                     /locus_tag="VNT12_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWI44873.1"
                     /translation="MKAMALAAGKGTRLFPLTGEIPKPMAPVVNTPIIEHIFDLMAGH
                     GIEEVYVNVHYLADALLQAYGEESRVNGMAVHLEREDELLGTAGGVKRLAGRFDDTFV
                     VVSGDALTDVNIEELVEYHREKGALATIALRRVYDTSEFGVVEVDEEGNIQGCQEKPS
                     PEEAISTLANTGIYVFEPQALDYIPEGVFFDFAEDVFPKLLEHGERFVGYQEDFY"
     gene            702..1595
                     /gene="murB"
                     /locus_tag="VNT12_01795"
     CDS             702..1595
                     /gene="murB"
                     /locus_tag="VNT12_01795"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWI44874.1"
                     /translation="MHANTLHRLFPSAKFDEPLKRYTAWKIGGPADVLLEPGSVHELM
                     EATEKAAEHGVPVTILGGGTNVLIRDGGIRGLTIRLAKSLTDVEVEGTRVTADAGVLY
                     PVLANTTAGRGLAGLEFATGIPGTVGGAVYMNAGAYGSETTEVLDWADVFRDHRVIRM
                     ENTELDLSYRHSALHENPHWVVLRAGYTLAPGDPLVLKARIKEFRAQRMNGSPNRPSC
                     GSTFKRPKGDFPGRVIEAAGLKGTRVGRIEVSPVHANYFVNLGDGTAQDALELIELVR
                     QRVIEKLGIELEPEVRVIGEP"
     gene            complement(1751..2332)
                     /locus_tag="VNT12_01800"
     CDS             complement(1751..2332)
                     /locus_tag="VNT12_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWI44875.1"
                     /translation="MEGRSTERVAVFVDGANLYHSIKNYYKGILDYARLLSAAVGDRK
                     LLRATFYIVEKQEADEGQGTTSARSFVYNLNKFGYKVRSKPLMVHETLTPDGERTVSH
                     KGDWDIGVVVDMMRLAGHADTYVLVSGDGDYVEAVEYLQSEKGIRVEVISAAQCTSQA
                     LLDVCDAHTDLADIPELFRRPGPPKAISDQQVS"
     gene            2473..2796
                     /locus_tag="VNT12_01805"
     CDS             2473..2796
                     /locus_tag="VNT12_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWI44876.1"
                     /translation="MADHAEGYSVVSVRGEMDLHTAPRFQYAIERAVEIEGVGAVVVD
                     LGDVAFMDSTALSALMRSKDTLGQRGISLRLAAPSEAVERIFSVTGFQDYFDIFPSRD
                     AAIPV"
     gene            2831..3052
                     /locus_tag="VNT12_01810"
     CDS             2831..3052
                     /locus_tag="VNT12_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HWI44877.1"
                     /translation="MKKTTVYLPEDLKSALGRLATERERSEAELIREAIRALVMDAEP
                     PRPRLPLFSSGDPTLAERVDEELAGFGEW"
     gene            3046..3471
                     /locus_tag="VNT12_01815"
     CDS             3046..3471
                     /locus_tag="VNT12_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWI44878.1"
                     /translation="MVIVLDTSGLLAAIDSSQRFHDAAKETLGGAVEPLILSPFVLAE
                     LDYLLTTRVGREAGLALLEEVARGTYRLAQFSIVDVAEARRIIVQYADFRIGLADTSN
                     VVLANRHDTLDVLTLDERHFRALRGPGGRPFRILPADAG"
     gene            3525..>4580
                     /locus_tag="VNT12_01820"
     CDS             3525..>4580
                     /locus_tag="VNT12_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44879.1"
                     /translation="MMADIKKLEVPVSLSRLGEVFRDAGHELYLVGGVVRGALLLGSD
                     TETATEAQINRQDADAATDAHPARIKALLRPHAENLWTVGERFGTIAATIGEYEVQIT
                     TYRTDQYTRGSRHPEVRFGDNLQGDLARRDFTINAIAADALTGEIVDPFEGREDLEKG
                     VIRAVGDPLERMRDDPLRMLRAVRFETTLSKPEKPFAMTQDLEEAIRNNAGWLESISA
                     ERKREEFEKILVSENVESGLRTLVRLGLMTYVVPEFMETVDVEQEAEFHHKDVFEHTL
                     IVVSNVEADPILRKAAFFHDIGKPRTLVYEQLCTYCGAKSTSKSAEEGECEHCGGRTI
                     PKRIHFYGHENVGAAIAR"
BASE COUNT          901 a         1392 c         1402 g          885 t
ORIGIN      
        1 gtaaaagtcc tcctgatacc ccacgaaacg ctctccgtgc tccagtaact tcgggaacac
       61 gtcttcggcg aagtcgaaga agacgccttc ggggatatag tcgagggcct gcggttcgaa
      121 gacgtagatg cccgtgttcg ccagggtgct tatggcctcc tcagggcttg gcttttcctg
      181 gcaaccctgg atgtttccct cctcatcgac ctcgaccacc ccgaactccg aggtgtcata
      241 gacccgccgc agggctatcg tggccagggc acctttctcc ctgtgatact ccaccagctc
      301 ctctatgttt acgtcggtca gcgcgtcgcc cgagacgacg acgaaggtgt cgtcgaacct
      361 gccggccaga cgcttcacgc cacccgccgt gccgagcagc tcgtcttccc tttccagatg
      421 tacggccatg ccgttcaccc tggactcttc gccgtaggct tgcaggagcg cgtcagccag
      481 gtagtggacg tttacgtaga cctcctcgat gccgtggccg gccatgaggt cgaagatatg
      541 ctctatgatc ggggtgttca ccaccggcgc cataggtttg gggatctcac cggtaagcgg
      601 gaagagcctg gttcccttgc ccgctgctag tgccatcgct ttcatagatc tctccctcac
      661 ccgttctgca aagcaattat cggtattcta tattccctct cgtgcatgcg aacactctac
      721 accggctctt cccttcggcc aagttcgacg aacccttgaa acgctacacg gcatggaaga
      781 tcgggggccc tgcagacgtc ctgctcgagc ccgggagcgt acacgagctg atggaagcca
      841 cggagaaggc cgccgaacac ggcgtacctg tcacgatcct cggcggtggt acgaacgtgc
      901 tcattagaga cggcggcatc aggggactca ccatcaggct cgccaaatcc ctgacagacg
      961 tcgaggtcga agggacgcgc gttaccgccg acgccggggt actctacccc gtgctcgcca
     1021 acaccacggc aggccgcggc ctcgccggcc tggagttcgc aacaggcatc ccaggcacgg
     1081 tcggcggcgc agtctacatg aacgccggcg cctacggtag cgagacgacc gaggttctcg
     1141 actgggccga cgtgttcagg gaccacaggg taatcaggat ggagaacaca gaactggacc
     1201 tgtcataccg ccacagcgcc ctgcacgaga acccacattg ggtcgtcctg cgggccggct
     1261 atacgctcgc cccgggcgac ccgctagtac tgaaggcccg cataaaagag ttcagggcgc
     1321 agcgtatgaa cggctcaccc aacaggccga gctgcggctc cacgttcaag cgccctaagg
     1381 gagacttccc gggccgcgtg atagaggcgg caggcctgaa aggtacgcgt gtgggccgga
     1441 tcgaggtctc cccggtgcac gccaactact tcgtaaacct cggggacggt actgcccaag
     1501 atgccctgga gctgatcgaa ctcgtccgcc aaagggtcat tgagaaactg ggcatcgagc
     1561 tggagccgga agtaagggta ataggggagc cgtagcaagt cagcatttca gcacgccgcc
     1621 tgacggcggc ttgtcagcac gctcaggcct ttggccctcg ctttcagcgt gtcagcaagc
     1681 acctgcccat gcgaaagctg acaagctgaa atgctgacaa gtcgcctggc gaagccgggc
     1741 gacttgctga ctagctgact tgctgatcgc tgatcgcttt gggtggccct ggccgcctaa
     1801 acaactccgg tatgtccgcc aggtctgtgt gggcgtcgca gacgtcgagg agggcctggg
     1861 aggtgcactg ggccgcgctt atgacctcga cgcggatgcc tttttcgctc tggaggtact
     1921 cgacggcctc gacgtagtct ccgtcgcccg agacgagaac gtaggtatca gcgtgccctg
     1981 cgaggcgcat catgtccacc acgacaccta tgtcccagtc gcccttgtgc gagaccgtcc
     2041 tctccccgtc cggggtcagg gtctcgtgta ccatgagcgg cttggagcgt accttgtagc
     2101 cgaacttgtt gaggttgtag acgaaagagc gcgccgaggt cgtcccctgt ccctcatcgg
     2161 cctcttgctt ctcgacgatg tagaaagtgg cccgaagtaa cttcctgtct ccgacggccg
     2221 ccgagaggag cctggcgtag tcgaggatgc ctttatagta gttcttgatc gagtggtaga
     2281 ggttagcccc gtcgacgaag acggccaccc gttctgtact tctcccttcc atcacgcccg
     2341 aatattaaca gaaaacattg catgccccta caagacggcc ggtttaccgg gcacacgaac
     2401 tacgctaata tccattggcc gctctgcatc gactctagag aggagtgagc ggacacggac
     2461 ttcgaggcca gcatagccga tcatgccgag ggatactccg tcgtctcggt gcggggtgag
     2521 atggacctcc ataccgcccc gaggttccag tacgccatag agcgggccgt cgagatcgag
     2581 ggcgtcggcg ctgtcgtcgt ggacctgggc gacgtcgcgt tcatggactc tacagccctc
     2641 tcggcgctca tgcgttccaa agacaccctc gggcaaaggg ggatctccct tcgcctcgcc
     2701 gcaccttctg aagccgtcga acgtatcttc tccgtgacag gcttccagga ctacttcgat
     2761 atctttccat cccgcgacgc cgccatcccc gtctgactct cgagcttaca actcacctct
     2821 gacgtatggt atgaagaaga ccactgttta tctaccggag gacctcaagt ctgcgctcgg
     2881 gcgtctcgcg acggagaggg agcgcagcga ggccgaactc atacgcgagg cgatcagggc
     2941 tctggttatg gatgccgagc cgccgcgtcc gcgattgccc ctattttcga gtggggatcc
     3001 gacactggct gagagggtag acgaggagct tgccggtttc ggggagtggt aatcgtcctc
     3061 gacaccagcg ggctgctcgc cgccatcgat tccagccagc gttttcacga cgccgctaaa
     3121 gagacgctgg gcggggcggt ggaacctttg atcctgtctc ccttcgtgct ggcagagctc
     3181 gactatctgc tgactacgcg ggtgggcaga gaggccggac tcgccttgct cgaagaagtc
     3241 gcccggggaa cataccgcct cgcgcagttc tcgatcgtgg acgtagcaga ggcaaggcgg
     3301 ataatagttc agtatgccga ctttcgcatc ggcctcgccg atacatccaa cgtggtgttg
     3361 gcgaaccgcc acgacacgct cgacgtgctt acgctcgatg aacgccactt cagggctttg
     3421 agggggccgg gtgggcgtcc gtttcgtatt ctgccggccg acgccggttg atcgtcgaga
     3481 gcttctgtga cctgcggtct gtcgcgtact agaatctggt cattatgatg gctgacataa
     3541 agaagctgga ggttccagta tctctctcgc gtctcggtga ggtgttcagg gatgccgggc
     3601 acgagctcta cctggttggt ggggtcgtgc ggggggcgtt gctcctgggc tctgatacag
     3661 aaaccgctac agaagcgcag atcaatcggc aggacgcgga cgcggcgacc gatgcgcatc
     3721 ctgcgaggat aaaggcgttg cttaggcctc atgccgagaa cctttggacg gtgggggagc
     3781 gcttcgggac catagcggcc accataggag agtacgaggt tcagatcacg acctacagga
     3841 ccgaccagta caccaggggg agcaggcatc ccgaggtacg cttcggagat aacctgcaag
     3901 gagatctggc acgcagggac ttcacgatca atgccatcgc tgccgatgcg ctcacgggtg
     3961 agatcgtcga ccctttcgaa ggacgcgaag atctggagaa gggtgtgatc cgggccgtcg
     4021 gagatcctct cgaaagaatg cgcgacgacc ccttgaggat gctgcgcgcc gtccgcttcg
     4081 agacgactct ttcgaagccg gagaagcctt tcgccatgac gcaggacctc gaagaggcga
     4141 tccgcaacaa cgccggttgg ctagagagca tctccgccga acgcaagaga gaggagttcg
     4201 agaagatcct ggtctccgag aacgtggagt ctggtctgag gacgctggtg cgtctcgggc
     4261 tcatgaccta cgttgtgccc gagttcatgg agacggtcga cgtcgagcag gaggccgagt
     4321 tccaccacaa ggatgtcttc gagcacaccc tgatcgtggt ctccaacgtc gaggccgacc
     4381 ccatcctgcg caaggcggct ttcttccacg acatcgggaa gcctaggacg ctcgtctacg
     4441 aacaactctg cacctactgc ggggcgaaga gcacaagcaa gagtgccgag gagggggagt
     4501 gcgagcattg cggagggcgc actatcccca agaggatcca cttctacggc cacgagaacg
     4561 ttggggccgc catagcgcgc
//
LOCUS       DATMJA010000079         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_721786, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000079 DATMJA010000000
VERSION     DATMJA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721786"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..477
                     /locus_tag="VNT12_01825"
     CDS             67..477
                     /locus_tag="VNT12_01825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWI44880.1"
                     /translation="MSTLEELNERGMGTLPGLIGLTILEAEEGRISSRLDLRNELMAP
                     NGYLHAATVVALADTSCGYGTFVNLPEGAESFTTIELKSNFVGTKRGGAIRCEAKLVH
                     GGRTTQVWDATVSDEEGGKPIALFRCTQMILYPC"
     gene            complement(505..1389)
                     /locus_tag="VNT12_01830"
     CDS             complement(505..1389)
                     /locus_tag="VNT12_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI44881.1"
                     /translation="MRPRDFAGLIMLGALWGGSFLFIRVAVPALGPFLLVELRVGLAA
                     TALFLFALAVGSMPKIRSRWRSFLVLGFLNAAVPFSLISAAEIHLTASLAAILNSTTV
                     MFTAIVAAVWMGDPLTARKTIGIVLGIVGVSVLVGWDPLPLNGIVLLAVVAMLLASLS
                     YALGATYAKRSFSGIPPLGMAIGQLGGAVVLLLPMAVVSVPERAPSTVVTLSMLGLAF
                     LSTAVAYLIYFRLIENVGPTSTVTVTLLVPVFGLLFGVLLLEEPFGFGTLAGLGVILT
                     SVVLITGIAPRKKRPEGN"
     gene            complement(1448..1966)
                     /locus_tag="VNT12_01835"
     CDS             complement(1448..1966)
                     /locus_tag="VNT12_01835"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWI44882.1"
                     /translation="MNVYVDEIESPAGPLAFAVNEDGALMRMHFVESDYGPVMEEDLE
                     DEGYAVEADETRTAGVRKELSEYVSGRRRTFDVPLAFAGSVWQKAVWMELTRIPFGET
                     RSYGEIADNLGRPGAARAVGRANASNVLPVVVPCHRVIAADGTLGGFNGGLHLKERLL
                     EHERNVLAAADS"
     gene            complement(1963..>2546)
                     /locus_tag="VNT12_01840"
     CDS             complement(1963..>2546)
                     /locus_tag="VNT12_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWI44883.1"
                     /translation="GHRVPGSVDGAELAFRAVLGQQVSVAGARTLAGRLVARFGEPLP
                     ETLAAKDGEPTHLFPGPHAVAGAALDGLGVPSARRESLRDLARALAGRGIVLDPGSER
                     EEVERRLLELRGIGPWTASYVAMRALRDPDAFLPSDLGIRKAICRLGHAGDRGSVASL
                     AERWRPWRAYATQHLWASLGDEKDKKEKGEVVA"
BASE COUNT          530 a          823 c          773 g          420 t
ORIGIN      
        1 caagcaacga gatacgcctc tgggataatc tttttagcca tgacaaagat attggaggcg
       61 ccgtagatgt ctactctcga ggagctgaac gaacgtggta tgggaaccct gcccggtctc
      121 atcgggctga cgatactgga agcagaggag ggtaggatct cgagccgcct ggatctccgt
      181 aacgagctca tggcccctaa cggctacctg catgctgcga cggtggtcgc ccttgcggat
      241 acctcgtgcg gctacggaac gttcgtgaac ctgcccgaag gcgcagagag cttcacgacc
      301 atagaactga agagcaactt cgtcgggacc aaacgtgggg gtgcgatcag gtgcgaagcg
      361 aagctcgtcc acggtggccg cacgacgcag gtgtgggacg cgacagtctc cgacgaggag
      421 ggtggcaagc ccatagcact cttccgatgc acgcagatga tcctctaccc gtgctgaacg
      481 cctggcgatg cgagcctgtg ctctctaatt accttcaggc ctcttcttcc ggggagcgat
      541 gcccgtgatg aggacgacgc tcgtgaggat gacaccgagc ccggctagcg taccaaaccc
      601 gaaaggctcc tcgagtagca acacaccgaa caacaacccg aagacaggca cgagtagcgt
      661 gacggtgacc gtgctcgttg gtccgacgtt ctcgatgagg cggaagtaga tcaggtaagc
      721 caccgcggtc gaaaggaacg ccagcccgag catggagaga gttacgacag tcgaaggcgc
      781 tctttcgggt acgctgacga cggccatggg caagaggagc actaccgccc cgcccagctg
      841 gcctatggcc attccgagtg gtgggatgcc cgaaaacgac cgcttggcgt aggtggctcc
      901 cagcgcgtaa gagagcgaag caagtagcat cgccaccaca gccaacagga cgatcccgtt
      961 caagggtagc gggtcccagc ccacaagtac actgaccccg actatcccaa gaactattcc
     1021 gatggtcttc ctggccgtca ggggatcgcc catccacacc gcggccacta tcgcggtgaa
     1081 cattacggtg gtcgagttca ggatggcggc tagggaggcc gtaaggtgta tctcggcggc
     1141 ggaaatcagg gagaaaggca cggccgcgtt caagaatccc aggacaagga acgacctcca
     1201 ccgactccgg atcttcggca tgctgccgac ggccagcgcg aagagaaaca gggccgtggc
     1261 agcgagaccg acccgcaact ctacgagcaa gaaaggaccc aacgcgggca cggcgacacg
     1321 gataaacagg aacgatccac cccatagcgc gcctagcatg atgagacccg cgaaatctct
     1381 cggtctcata agccgcaaat ttcagatatg acgaacgttg tgttgccctc tcgccacatc
     1441 cgacaaacta gctgtccgcc gcggcgagta cgttgcgctc gtgctcgagc aaacgctcct
     1501 tgaggtgtag gccaccgttg aacccgccga gcgtgccgtc cgcagcgata actcggtggc
     1561 agggtacgac cacgggcagg acgttggagg cgttggccct gccgacggcc ctggccgcgc
     1621 ccggccggcc gaggttgtcc gcgatctccc cgtaggacct ggtctcccca aaggggatgc
     1681 gcgtcaactc catccaaacg gccttctgcc acaccgagcc ggcgaacgcg agcggcacat
     1741 cgaacgtcct gcgccgtcca ctgacatact cggacaattc ttttcggacg ccggcggtcc
     1801 tggtctcgtc cgcttctacg gcgtaccctt cgtcctccaa atcctcttcc atgaccggcc
     1861 cgtagtcgct ctctacgaag tgcatccgca tcagggctcc atcttcgttc accgcgaacg
     1921 cgagcgggcc tgctggagac tctatctcgt cgacgtaaac gttcacgcta ccacctctcc
     1981 tttctctttc ttatccttct catccccaag gctggcccac agatgctgcg tggcgtaggc
     2041 gcgccagggt ctccaccgtt ctgcgagaga tgcgacgctc ccccggtcgc ccgcgtggcc
     2101 gagacggcag atggcctttc tgatcccgag gtccgaggga agaaacgcgt cggggtcgcg
     2161 gagcgcccgc atcgcaacgt acgaagcggt ccacggacct atcccccgca actccagcaa
     2221 ccgccgttcc acctcctcac gctcagagcc agggtcgagc acaatcccac gtcctgcaag
     2281 ggcgcgcgca agatcgcgca agctttcacg acgcgcactg ggaacgccga gcccatcgag
     2341 tgccgccccg gctacggcgt gcggaccggg gaacagatgg gtaggttcgc cgtctttagc
     2401 tgcgagcgtc tcggggagcg gttctccgaa gcgggcgaca aggcggccgg cgagggtgcg
     2461 ggcgccggcc acggagacct gctggccaag caccgcccgg aaggcgagtt cggcaccgtc
     2521 cacacttccc ggtacgcgat gccccg
//
LOCUS       DATMJA010000080         2735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_781190, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000080 DATMJA010000000
VERSION     DATMJA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781190"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VNT12_01845"
     CDS             <1..531
                     /locus_tag="VNT12_01845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWI44884.1"
                     /translation="EGHLQLSARREISALSGFLGRTSLLTRLFPKSSIVKSQFPAVTD
                     QSHPTSVDWVSGACMVVRRETLRDVGPLDERFFMYFEDADLCRRARAAGWLVYYLPHV
                     EIVHQTGASSRSKPKAIWLLHKSAFLYHRKHGVHGRFGAYSAMVLAGLVLRALAKLGI
                     SLLQRVAEGLAFRNPR"
     gene            complement(579..1139)
                     /gene="rfbC"
                     /locus_tag="VNT12_01850"
     CDS             complement(579..1139)
                     /gene="rfbC"
                     /locus_tag="VNT12_01850"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWI44885.1"
                     /translation="MNVVETVLSGVRIIEPDVFGDARGFFMESWNGARYEEHGIPDRF
                     VQDNLSFSAHGVLRGLHFQNPRAQGKLVSVLRGEVFDVAVDIRVGSPTFGGWSGSTLS
                     EENKRQLWVPPGFAHGFVVTSESALFSYKCTDYYAPEYDRSILWDDPEIGIQWPVENP
                     SLSDKDRAAPPLNRMPADTLPHYTGD"
     gene            complement(1136..2017)
                     /gene="rfbA"
                     /locus_tag="VNT12_01855"
     CDS             complement(1136..2017)
                     /gene="rfbA"
                     /locus_tag="VNT12_01855"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HWI44886.1"
                     /translation="MKGIVLAGGSGTRLYPLTRAVSKQLLPVYDKPMIYYPLTTLMLA
                     GIRDILVISTPEDLSRFEDLLGDGSQWGVDFSYAVQPRPEGLAQAFVIGREFVGSESV
                     SLVLGDNIFFGHGFSELLERSAGRHRGATVFGYYVRDPGRYGVVEFDGDGTVLSIEEK
                     PERPRSHYAVTGLYFYDNQVLDIAANLEPSARGEYEITDVNLEYLDRGQLRVEPLGRG
                     AAWLDAGTHESLQQASNFIETIEQRQGLKIGCPEEISYRKGYIDAERLERLAHPLRKN
                     DYGRYLLELLKSEEYVT"
     gene            complement(2014..>2735)
                     /locus_tag="VNT12_01860"
     CDS             complement(2014..>2735)
                     /locus_tag="VNT12_01860"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWI44887.1"
                     /translation="WGNLERDDHDAFRFLHISTDEVYGTLGPTGYFTEESPFRPNSPY
                     AASKAASDHLVRAYHHTYGLPTLTTNCSNNYGPYHFPEKLIPLMILSALRGEPLPVYG
                     DGQQIRDWLYVEDHCRAILAVLQRGRPGETYNVGGHNERTNLEVVKTICALLDGLVPG
                     SPHESLITFVADRPGHDRRYAIDAGKIERELGWRPEETLETGMEKTVRWYLENRGWCE
                     AALSRGYRGERLGLGSRGGPA"
BASE COUNT          471 a          885 c          855 g          524 t
ORIGIN      
        1 gaaggtcatc tccagctctc ggcgcggcgg gagatcagcg ccctctcagg gttcctcggg
       61 cgcacctccc ttctgacccg cctcttcccc aagagctcta tcgtaaagag ccagttcccc
      121 gccgttacgg accagtctca cccgacctcg gtcgactggg tctccggcgc ctgcatggtc
      181 gtccgcaggg agacattgcg agacgtaggc cctctggacg aacgcttctt catgtacttt
      241 gaggacgccg acctctgcag gcgcgcccgc gcggcaggct ggctcgtcta ttacctgccg
      301 cacgtcgaaa tagtccacca gaccggggca agcagccgca gtaaacccaa ggcgatctgg
      361 ctcctccaca agagcgcttt cctctaccac cgcaaacacg gcgtccacgg ccgcttcggc
      421 gcctacagcg cgatggttct cgccggactc gtcctccgcg ccctggcgaa gctgggaatc
      481 tcgctgctac agcgggttgc ggaaggcctg gccttccgca acccgcgcta gtcagcctgt
      541 cagcacttca gcttgtaagc ttggtgatct cgccagcctc agtcgcccgt atagtgcggc
      601 agggtgtctg ctggcatcct gttcaggggt ggggcggcgc ggtctttgtc cgagaggctc
      661 gggttctcga cgggccactg tatcccgatt tcgggatcgt cccacaggat ggaccggtcg
      721 tactcgggag cgtagtagtc tgtgcatttg taggagaaga gcgcgctctc gctcgtgacg
      781 acgaaaccgt gggcgaagcc gggagggacc cagagctggc gcttgttctc ttctgagagg
      841 gtcgagcccg accacccgcc gaaagttgga gagccgaccc tgatatccac ggcgacgtcg
      901 aagacctcgc cccgcaagac ggagacgagc ttgccctggg cccttgggtt ctggaagtgg
      961 agaccgcgaa gcacaccgtg ggccgagaac gagaggttgt cctgaacgaa gcggtccggt
     1021 atgccgtgtt cttcgtagcg ggcaccgttc cagctctcca tgaagaaccc tcgggcatct
     1081 ccgaagacat ccggctcgat aatgcgtact cctgacagta ccgtctcgac gacgttcaag
     1141 tgacgtactc ctcgctcttc aggagctcga gcaggtaccg gccataatcg ttcttcctca
     1201 gagggtgggc gaggcgttcg aggcgttcgg cgtcgatgta acctttgcgg taggagatct
     1261 cctcgggaca ccctatcttg aggccctgcc gctgctcgat ggtctcgatg aagttggacg
     1321 cctgctgtag cgactcatgg gtccccgcgt ccaaccacgc cgctccgcgg cccaggggct
     1381 cgacgcggag ctgcccgcga tctaggtact ctaggttgac gtccgtgatc tcgtactccc
     1441 cccgcgctga aggctcgagg ttcgcggcta tgtccaggac ctggttgtcg tagaagtaga
     1501 ggcctgtcac ggcgtagtgc gaacgaggcc tttccggctt ctcctcgata ctgagaaccg
     1561 tcccatcgcc gtcgaactcg acaacaccgt agcgccccgg gtccctgacg tagtacccga
     1621 agacggtggc ccctctatgc ctccccgccg accgctccag gagctcggag aaaccgtgcc
     1681 cgaagaagat gttgtccccg aggacgaggg agacgctctc ggacccgacg aactctcgcc
     1741 cgatgacgaa ggcctgggcc aggccttctg gcctgggctg cacggcgtag ctgaaatcta
     1801 caccccactg cgaaccgtcg ccgagcagat cctcgaagcg agaaaggtct tcgggggtgg
     1861 agatcacgag tatatccctg atgcccgcga gcatcaacgt ggtcaatgga tagtagatca
     1921 tcggcttgtc gtagacggga aggagctgct tgcttaccgc cctcgtgagc ggatacagcc
     1981 gcgtacccga gccccctgcg agcacgatcc ccttcacgcc ggccctccgc ggctgcccaa
     2041 ccctagccgc tctcccctgt agccccgcga gagcgcggcc tcgcaccacc cgcggttctc
     2101 caggtaccag cgcacggtct tctccatgcc cgtctccagc gtctcctcgg gcctccagcc
     2161 gagttcgcgt tctatcttcc ccgcatctat ggcatagcgt ctgtcgtggc ccggcctgtc
     2221 agcgacgaag gtgataagcg actcgtgcgg cgatccagga accagcccgt cgagcagggc
     2281 gcagatggtc ttcacaactt cgaggttcgt ccgctcgttg tggccgccga cgttgtacgt
     2341 ctcgccagga cgaccgcgct ggaggacagc gaggatagcc ctgcagtggt cctccacata
     2401 gagccagtcc ctgatctgct gcccgtcgcc gtagacgggc aggggttctc ctcgcagggc
     2461 gctcaggatc atgagcggta tgagcttctc ggggaagtgg tagggaccgt aattattgga
     2521 gcagttggtc gtcaacgtgg ggagcccgta cgtatggtgg taggcccgca cgaggtggtc
     2581 ggaggcggcc ttggaggcgg cgtaggggga gttgggtcgg aagggactct cctcggtgaa
     2641 gtagcccgtc ggccccagcg tgccgtacac ctcgtccgtc gagatgtgca gaaagcggaa
     2701 ggcgtcgtgg tcgtcccgtt cgaggttgcc ccagt
//
LOCUS       DATMJA010000081         5254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_425695, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000081 DATMJA010000000
VERSION     DATMJA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425695"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VNT12_01865"
     CDS             <1..870
                     /locus_tag="VNT12_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWI44888.1"
                     /translation="GIINDPVLLRLDENHFWLSLADSDVLLWAQGLAYNMGLDVEIKE
                     PDVGPMQVQGPKSREVMTDLFGDEILEVPYYYLVHRELDGMELVISRTGYSGELGYEL
                     YLKNASHDGLKLWDRMLQAGEPHGISPIGPCHIRRIEGGILALGCDMWYDTNPYEVGY
                     GYKWMVELEQEQDFVGREALKRISEEGVSRRLVGVELGGSNLGSYNDGSMIDYFPVHK
                     DGERIGKVTSACYSPRLQKNIGFAMVPIEYSELGTGLEVERPEETVAATVADRVFFKP
                     EKAEQNLGAASAS"
     gene            890..1780
                     /locus_tag="VNT12_01870"
     CDS             890..1780
                     /locus_tag="VNT12_01870"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWI44889.1"
                     /translation="MGRKNVSTEQNTELRQRANRRLAEFLRHPGYEVIPLQGTEEAVI
                     EHVPKDIALTVTASPGKGTWATVELAERLAGHGFGVAPHLSARLIRDGSELGEILQSL
                     REAGIRDAFVVAGDVDEPAGEFEGAAELLQAMSEIGHGLDEVGITGYPESHPKISDEV
                     TIRAMYDKAPYATYIISQVCFDPEVISDWARRVWSRGVKLPIRVGMPGYVNRQKLVRI
                     SASIGVGESARFLRRQRNWLLKMFLPGGYSPDRLIEGLKPGFADPASNIQGLHIYTFN
                     EVETTEMWRREMLERAETST"
     gene            1796..2404
                     /locus_tag="VNT12_01875"
     CDS             1796..2404
                     /locus_tag="VNT12_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HWI44890.1"
                     /translation="MDFSPVDTTSEYLDLPLRGFLDLVASGRPAPGGGSVAAIAVALA
                     AGLSGMAARLSAGQLVDAEGLADRADAARRRVAPLARIDAESYGRVLDAYREPDSEIR
                     RERVSDALSGATDVPLAVAEIGHEVAGIAARLVEEGNPNLEGDAIAAVLLAEAGVRAA
                     TVLVEINLSSANVEDDRLGRANKLVDKTASTARRVTGGDRRG"
     gene            2463..3764
                     /gene="gcvT"
                     /locus_tag="VNT12_01880"
     CDS             2463..3764
                     /gene="gcvT"
                     /locus_tag="VNT12_01880"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWI44891.1"
                     /translation="MPERRTPFYDFHRRSARNLVKGGGDFMFPTSYASPLEEHLNVRR
                     NVGMQDLSTMGEVDIKGPGAERLVRRLLVNEVQDMEPGQLRYSTMCNEEGGIVDDVTV
                     YKFDDEHFMIVTSSGPRKKAFRWISEHAVGSSAYVTDMTAAVALPVVQGPRSRAFLKT
                     MAEGVDLDELRFFRFAPCRIGDIELFISRSGYTGELGYELYTPADQAAALWEHILQTS
                     QKFNLKPYGLEAMQSLRIEKGLPLYGPDISEQDTPFHVGLGRWVRFDKRDFIGREALL
                     RVQERGLERRWVGLTLESEVPAAAGAGVYGIADVATFREVVETGAEAGEHEDEFMPGE
                     RQVGRVTSSAMGHTVGRMLAMAYVDTAHSWPGNNLIVEFGGRPVPARVAETPFFDPEN
                     ARVRAKPTEDEQLSELVPEPARLTNARPRTPVRQSPDGYTR"
     gene            3764..5011
                     /gene="solA"
                     /locus_tag="VNT12_01885"
     CDS             3764..5011
                     /gene="solA"
                     /locus_tag="VNT12_01885"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWI44892.1"
                     /translation="MNVQYSGRYDAIIVGVGGMGSATAYYLARRGRRVLGLEQFGIPH
                     AMGSSHGHTRIIRLAYYEDPSYVLLLRRAYELWREIQSKAGEKLLHITGSIDAGPEES
                     WVFKGSWESCKLHDLPHEVVTGAELVRRYPGYHLPPEHLALIQPEGGFLTPERCIVSY
                     VMAAQSLGAEIHGHERVLEWVPLEGGVRVRTDRGTYEADRLVMTAGAWNGDLLDVLDG
                     LAVPERQVLAWLQPDRPDLFRPDTFPVFNLLVDEGRFYGFPVFSVPGFKFGKYFHLGE
                     TGEADEIDRQPHDYDEQLLRSFAERYFPDGCGPTMDLQTCMFTNTPDHHFVIDLHPDY
                     PQVSIASPCSGHGFKFASVVGEIMADLAEKEFTRHDISLFRLDRLRRQATGYQSVSGG
                     QPAEQSDGRPRSFTRMGDIRTFW"
     gene            complement(5019..>5254)
                     /locus_tag="VNT12_01890"
     CDS             complement(5019..>5254)
                     /locus_tag="VNT12_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWI44893.1"
                     /translation="LLFWGAVAWFVVFSTFGGYGFYWLNLKLSSVARVSSLIYLTPPT
                     TMVWAYLMFGERVGLLAVVGLVICFGGVLLARG"
BASE COUNT         1035 a         1608 c         1714 g          897 t
ORIGIN      
        1 ggcatcatca atgaccccgt gctgttgcgt ctcgacgaga atcatttctg gctctccctg
       61 gccgacagcg acgtgttgct ctgggcgcag ggtctcgcct acaacatggg gctggacgtc
      121 gagatcaagg agcccgacgt cggcccgatg caggttcagg ggccgaagtc cagggaggtg
      181 atgaccgacc tcttcggcga cgagatactc gaggtgccct actactattt ggtgcacagg
      241 gaactcgacg ggatggagct cgtgatctcc cgcaccgggt actcagggga gcttggatac
      301 gaactctacc tgaagaacgc gagccacgac gggctcaagc tctgggaccg gatgttgcag
      361 gctggcgagc cgcacgggat ctccccgatc ggaccgtgcc acatccgcag gatcgagggc
      421 ggcatcctgg ccctgggctg cgacatgtgg tacgacacca acccctacga ggttggatac
      481 ggctacaagt ggatggtcga gttggagcag gagcaggact tcgtcggaag ggaggcgctg
      541 aagaggatca gtgaggaggg cgtcagccgc aggctggtcg gcgtcgagct agggggtagc
      601 aacctcggtt cgtacaacga cggctccatg atcgactact tcccggtcca caaagacggc
      661 gagaggatcg gcaaagtcac ctcggcctgc tattcgccgc gcctgcagaa gaacatcggg
      721 ttcgccatgg tgcccatcga gtacagcgag ctgggaaccg ggctcgaggt cgagcggccc
      781 gaggagacgg tcgcagccac cgtggccgac cgtgtcttct tcaaaccgga gaaggccgag
      841 cagaacctgg gcgccgccag cgcgtcttag ggggccagag agaagcgacg tgggccggaa
      901 gaacgtgtcg acggagcaaa acaccgaact cagacagcgg gcgaaccgga ggttggcaga
      961 gtttctgcgg catcccggct acgaggtgat acccctccag gggacggagg aagcggtcat
     1021 cgagcatgtc ccaaaggaca tagcgcttac ggttacagcc tcgcctggta agggaacctg
     1081 ggctacggta gagctcgccg agcggttggc gggacacggc ttcggcgttg cgccccacct
     1141 ctcggcgcgg ctcattcggg acggatcgga actcggtgag atcctacaga gtctgcgcga
     1201 agccggcata agggacgcct tcgtcgtcgc cggggacgtg gatgagcccg caggggagtt
     1261 cgagggcgcg gccgaattgc tccaggcgat gtcggagatc gggcacggcc tcgacgaggt
     1321 tggcatcaca ggctacccgg agagccaccc caagatcagc gacgaggtga cgatccgggc
     1381 catgtacgat aaggcgccct acgccaccta catcatcagc caggtctgct tcgatccgga
     1441 ggtgatctca gactgggcca ggcgggtgtg gtcgcggggt gtgaaactcc cgatacgcgt
     1501 aggcatgccg ggttacgtga atcgccagaa gctcgtacgc atctctgcga gcataggtgt
     1561 cggtgaatcg gcgcgtttct tgaggagaca acgtaactgg ctgctcaaga tgttcctacc
     1621 cggtggttac agcccggacc gcctgatcga aggcctgaaa cccggctttg cagaccctgc
     1681 cagcaacatc caaggcctcc atatctatac attcaacgag gtggagacaa ccgagatgtg
     1741 gcggcgtgag atgctggagc gcgccgaaac gtccacttga tgggcttgta gtagtatgga
     1801 cttctcacca gttgacacga catcggagta tctggacctt ccactaagag gcttccttga
     1861 tctcgtggcc tcgggcaggc cggctccagg cggaggctcc gtggccgcca tagccgtggc
     1921 cctcgccgcc gggttgtcag gcatggccgc ccgcctgtcc gcaggccaac tagtcgacgc
     1981 cgaagggctg gccgaccgtg cggatgccgc gaggcggagg gtagcacctc tcgccaggat
     2041 cgacgcggag tcatacgggc gcgtcctcga cgcttaccga gagcctgact ctgagatccg
     2101 cagggagcgc gtcagtgatg ccctctcggg ggctaccgac gtgccgctgg ccgtcgcgga
     2161 gatcgggcat gaggtcgcgg gcatcgcggc ccggctggtg gaggagggca accccaacct
     2221 ggagggagac gcgatcgccg cagtcttgct cgcagaagca ggagtacgcg ccgctacggt
     2281 attggtcgag atcaatctgt cttcggcaaa tgtggaggat gaccgtctcg gacgggcgaa
     2341 caaactcgtc gacaagactg cgtcgactgc ccggagggtc accggaggcg ataggcgagg
     2401 gtgaccgtgg ccagtgcttg cagtatagtt tacaaacaat cttcctgaga gaggagaagg
     2461 tcatgccgga gagaagaacg ccattctacg atttccaccg ccgttcggcc aggaacctgg
     2521 tcaagggagg cggggacttc atgttcccca cctcctacgc ctcgccacta gaagagcacc
     2581 tgaacgtgcg caggaatgta gggatgcagg acctatcgac tatgggggag gtggacatca
     2641 aggggcctgg cgccgagcgg cttgtgaggc gtctgctggt gaacgaggtc caggacatgg
     2701 agcctgggca gctcaggtac tcgacgatgt gcaacgaaga gggcggcatc gtagacgacg
     2761 tcacagtcta caagttcgac gacgagcact tcatgatcgt caccagttcc gggccccgca
     2821 agaaggcatt tcgttggatc tccgagcacg ccgttggctc cagcgcctac gtgaccgaca
     2881 tgacggcggc cgtcgccctg cctgtggtcc aggggccgcg ttcgcgcgcc ttcctcaaga
     2941 ccatggccga aggcgtggat ctcgacgagc taaggttctt ccggttcgcg ccgtgtcgga
     3001 tcggggacat agagctgttc atctcacgct caggctatac cggcgaactg ggctatgaac
     3061 tgtacacacc agcggatcag gcggccgcgc tgtgggagca tatcctgcag acatctcaaa
     3121 agttcaacct gaagccctat ggcctcgagg ccatgcagtc actgcgcatc gagaagggtc
     3181 ttcctctcta cggccctgac ataagcgagc aagacacccc tttccatgtg ggtctcggtc
     3241 gctgggtgcg gttcgacaag cgcgacttca taggtcgcga ggccctgctg cgggtgcagg
     3301 aacgtggcct ggagagacgc tgggtcggcc tgaccctgga gagcgaggtc cccgccgccg
     3361 cgggcgccgg ggtctacggc atcgccgatg ttgccacctt cagggaagta gtggagaccg
     3421 gggccgaggc gggcgagcac gaggacgagt tcatgccggg cgaacgtcag gtcgggcgcg
     3481 tcacttcgag cgccatgggg cataccgtcg gcaggatgct ggccatggca tatgtagata
     3541 ccgcccactc ctggcccggc aacaatctga tcgtcgagtt tggcggcagg cccgtccccg
     3601 ccagggtcgc cgagaccccc ttcttcgacc cggagaacgc ccgcgtcagg gccaaaccta
     3661 ctgaagacga gcagctctcc gaactggtcc ctgagccggc gcggctgacg aacgccaggc
     3721 caaggacgcc tgtgagacaa tctcctgacg ggtacaccag ataatgaacg tccagtactc
     3781 cggtcgctac gacgctatca tcgtcggcgt gggggggatg ggtagcgcca ccgcctacta
     3841 tctcgccagg aggggcaggc gggtgcttgg tctggagcag ttcggtatcc cgcacgcgat
     3901 gggttcctcg catgggcata cgcgcatcat ccgcctggcc tactacgaag acccctccta
     3961 cgtgttgctc ctcaggcgcg cctacgagct gtggcgtgag atccagagca aggccggcga
     4021 gaagctcctg catatcacgg gctccattga tgccggaccg gaggaatcct gggtcttcaa
     4081 gggctcgtgg gagtcctgca agctccacga ccttccgcac gaggtagtga caggcgcgga
     4141 gctcgtgcgg cgctaccccg gctatcatct tccccctgaa cacctcgcgc tcatccagcc
     4201 cgagggaggt ttcctcacgc cggagcggtg catcgtctcc tacgtgatgg cggcgcagtc
     4261 cctgggcgcc gagatccacg gccatgagcg agtcctcgag tgggttccgc tggagggagg
     4321 ggtacgcgtc cgtaccgacc gcggcacata cgaggccgac aggctcgtga tgaccgccgg
     4381 cgcctggaat ggggatctac tcgacgtcct cgacggactc gccgtgccag aaaggcaggt
     4441 cctcgcctgg ctgcagccgg acaggccgga tcttttccgg ccggacacct tccctgtctt
     4501 caacctgctc gtggacgagg gccgcttcta cggcttccct gtcttcagcg tgccaggctt
     4561 caagttcggc aaatacttcc acctgggcga gaccggcgag gccgacgaga tcgaccgcca
     4621 gccccacgat tacgacgagc agctcctcag gagcttcgcc gaacgatact tccctgacgg
     4681 ctgcggcccc acgatggact tgcagacctg tatgttcacc aacacccccg accatcactt
     4741 cgtcatagac ctgcaccctg attacccgca ggtctctatc gcctcgccct gctccgggca
     4801 cggcttcaag ttcgccagcg tcgtcggcga gatcatggcc gacctggccg agaaggagtt
     4861 tacccgtcac gatatctccc tcttccgtct cgaccggcta aggcgacaag ccaccggata
     4921 tcagtcggtc agcggcgggc aaccggcgga gcagtccgac ggtcgtcctc gctccttcac
     4981 caggatggga gacataagaa cgttctggta gccgcgggct agcctctcgc caacaggacg
     5041 ccgccaaagc agatcacgag cccgacaacg gcgagcagac cgacacgctc tccgaacatc
     5101 aggtaggccc agaccatggt ggttgggggt gtgaggtaga tgaggctgct caccctggcc
     5161 acgctgctca acttcaggtt cagccagtag aagccgtaac ccccgaacgt ggagaaaacg
     5221 acgaaccagg cgacagcacc ccagaagagc aacc
//
LOCUS       DATMJA010000082         2989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_247949, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000082 DATMJA010000000
VERSION     DATMJA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247949"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2643
                     /locus_tag="VNT12_01895"
     CDS             <1..2643
                     /locus_tag="VNT12_01895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWI44894.1"
                     /translation="LDAQVVIMGRGDEHYERALEEAVSRHPGVVAYHATSEEALARQV
                     YAGSDFFLAPSTFEPCGLGPLIAMRYGSIPIARRTGGLAETIRDNTRHPESGLGFTFA
                     RRSPEALVKAVSRALETYSRKSEWEALQGRAMGANFSWEDAGRAYERLYARALAGRRT
                     TALAKTKGAPLTHVGFRGRTRSKQAVPLALVHHANQYLITDGYDNREGITEIVEGYAA
                     ALRLHEEYGIPASLHLSGTLIETLAWHYPWFLELVRDLREKGLISLIGGTYSENVMPL
                     FEPSFNSRQLDEFLWLYRRHLGRPPEEVEICWVPERVWDTEKLAATLTSSELANGGYR
                     FVLLDDRLLYPTNGSYEGSPRARFDSIGPFDSNASRSHSYDKPAIAETYRTYRIAEAR
                     GLVMVPISANLRYWVPPSFPEHWRRLEKTVKSLSRNGGDDTLLVYADDLEITAGVGGW
                     DASALGRYDAFLRWVASREDVAPVLMSEWLAGHPPQEERRLEPGTFFEVAQQWEAGED
                     YSGWWENDAWSPYRERLVHAQKALRQAQQEGVDRRLLDLAWKHLLASAYETAWYDPVE
                     DGTVAAPFAKAVASHARACLVMVDAARWFARTERSPSAEVMDIDEDGEDEVILKGEHL
                     YAVIAPEHGGRLIYLFTLTPEGGALVVGNPTDDWNFKEELNRYMDNPPNHPGALADAG
                     FEHDRYLVSALSYGTHAWVEMTNVQEGSRLFGTRKSVLLAPGHPALAVCYRMREESGN
                     LATETCLAPDYYRLLREGRRGLSPYNGESWRGYRNGGVAVWLGLAGDEETGWTEPARA
                     EAGHGMNVRVQADTSHFHLLISCGDTDEDSCQQLLKENRATLNEIATTAHLTHYGFPA
                     ARRHPIRVPSNDFLMCSGRRGPRG"
BASE COUNT          556 a          964 c          976 g          493 t
ORIGIN      
        1 ctggacgctc aggtcgtgat aatgggccgg ggagatgagc actacgagcg ggcgctggag
       61 gaagcggtgt cccgtcaccc cggcgtcgtc gcctaccacg ccaccagcga ggaagcgctg
      121 gcgaggcagg tgtacgctgg gtcggacttc ttcctggccc cttccacctt cgagccttgt
      181 ggccttggac ctctgatcgc gatgcgctat ggctccatcc ctatcgcccg tcgcaccggc
      241 ggcctggccg agaccatcag ggacaacacg cgacacccgg aatccggcct gggcttcacc
      301 ttcgcccgcc gctctccgga ggccctggtg aaagcggtga gcagagccct ggagacatat
      361 agccgcaaga gcgagtggga ggctctgcaa gggcgggcca tgggcgccaa cttctcgtgg
      421 gaagacgctg gccgtgcgta cgagcggctc tacgcgcggg ccctcgccgg acgccggacg
      481 accgccctcg ccaaaaccaa aggtgcgcct ctcacccacg tgggattccg ggggaggacg
      541 cggtccaagc aggccgtgcc cctggctcta gtgcaccacg ccaaccagta cctcatcacc
      601 gacggctacg acaaccggga gggaatcaca gagatagtgg agggctacgc ggcagcgctc
      661 aggctgcacg aggagtacgg tatccccgcg agcctccatt tgagcggaac cctgatcgag
      721 acgctcgcct ggcactaccc gtggttcctg gagctggtga gggacttgcg ggagaaggga
      781 ctcatctccc tgataggcgg aacgtactcc gagaacgtga tgcctttgtt cgagccttcg
      841 ttcaactcgc ggcagctcga cgagttcctc tggctctatc gccgccacct gggacgcccg
      901 ccggaagagg tcgagatctg ctgggtgccg gaacgcgtct gggacaccga gaagctggcc
      961 gccaccctga ccagcagcga actggcaaac ggtggctacc gcttcgtcct gctggatgac
     1021 cggctgctct accccaccaa cggctcatat gagggtagcc cgcgcgcacg attcgactcg
     1081 atcggaccgt ttgactcgaa cgccagccgg tcccattcgt acgataagcc cgctatagcc
     1141 gaaacctaca ggacctaccg catcgccgag gcgaggggcc tggtgatggt gcccatctcg
     1201 gccaacctgc gctactgggt accgcccagc ttccccgagc attggcggcg cctggagaag
     1261 acggtcaaat cgctcagccg gaatggcggg gacgacaccc tcctcgtata cgccgacgac
     1321 ctggagataa cggcgggcgt cggcggttgg gacgccagcg ccctcggacg ctacgacgct
     1381 tttctgcgct gggtagcgtc ccgagaggac gtggctcctg tcttgatgtc cgagtggctg
     1441 gccggacacc caccgcaaga agagcggagg ctggagcccg gcaccttctt cgaggtggcg
     1501 cagcaatggg aggcgggcga agactacagc ggatggtggg agaacgacgc atggtcgccc
     1561 tacagggagc gattggtcca cgcccagaag gcgctgcgcc aggcgcagca ggagggcgta
     1621 gaccggcggc tgttggatct cgcctggaaa cacctcctcg cttccgctta cgaaaccgcc
     1681 tggtacgacc ccgtcgagga cggcactgtc gccgctccct ttgccaaggc ggtggccagc
     1741 cacgcccgcg cctgcctggt aatggtagac gccgcccggt ggttcgccag aacggagcgg
     1801 tctccgtccg cggaagtcat ggacatagac gaagacggcg aggacgaagt gatcctgaag
     1861 ggcgagcacc tctacgccgt aatagcgccc gagcacggcg ggaggctcat ctacctcttc
     1921 acgctaacgc ctgaaggcgg cgccctggtc gtgggcaacc ccaccgacga ctggaacttc
     1981 aaggaggagc tcaaccggta tatggacaat ccgccgaacc atccgggcgc gctagccgat
     2041 gcgggtttcg agcacgaccg ctacctggtt tccgccctca gctacggcac tcatgcctgg
     2101 gtggagatga ccaacgtcca ggagggcagc cgcctgttcg ggactcgcaa gagcgtgctc
     2161 ctggcgcccg ggcaccctgc cctcgcggtc tgctaccgga tgcgcgagga gtccggcaac
     2221 ctcgccacgg agacgtgcct cgccccagac tactaccgcc tgctacgcga aggtcgccgc
     2281 ggcctgagcc cgtacaacgg cgagagctgg cgcggttatc gcaacggagg tgtcgccgtc
     2341 tggctggggc tagccggcga cgaagaaacc ggctggacgg aaccggccag ggcggaggcg
     2401 ggacacggga tgaacgtccg cgtgcaggcc gacacctctc atttccacct cctgatctcc
     2461 tgcggggaca cagacgaaga ctcgtgccaa caactcctca aggagaacag ggctacgctc
     2521 aacgagatag cgaccaccgc gcatctcaca cactacgggt tcccggctgc caggcgacac
     2581 ccaatccgcg ttccatcgaa cgacttcctg atgtgctcgg gaagaagggg tccgcgtggt
     2641 tagcgcacgc aaagaatatt accggaacac gaacagaatg atagctttgt tactcaatcc
     2701 tttcgggacg gcgctagtcg gttgtgctat ctcctctgtt gcgaagttgg gcgggcccgt
     2761 gcaggttagt ctcgtggacg gtgtcctgtc atgtggtaca ggggagagga gatgctgaga
     2821 ggtatattcg gacggttcac ctcttcgaag ggtcagggcg agcggtctgc gctcgtagcc
     2881 ctacggacct ttctgccttt catggggcct cactggcgag ggttcgtccc ggcggtgatc
     2941 ggggtcatcg cggccagcct ggtcgccctg ctaaagccct ggccgctgg
//
LOCUS       DATMJA010000083         9019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_11320, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000083 DATMJA010000000
VERSION     DATMJA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11320"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2164
                     /locus_tag="VNT12_01900"
     CDS             <1..2164
                     /locus_tag="VNT12_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWI44895.1"
                     /translation="TVDRIDSILSANDLVADPVKKDAIDQADSRGETFSTLFVLFGQF
                     SVAAGMLLIFLIFVMLAAERKHELGIARAVGMQRGALVRAFAFEGALYALVASTIGSV
                     AGVGVGWVMVRFLGRGFTGGREDFQIVFATSPQNVILAFCMGMVLTFIVVLISSWRVS
                     RLNVVRAIRDIPEPDKRGRTVMGVIVAVLLPLAGAIVFWQGIETRTTAFYLGGLSLLI
                     IGAALLARVLGVTDRVAFSVSGLALLALWLTPASITTPADMARGPEMFFVSGIALVVA
                     GVWLVVFNADVVLRVVVALFGRMRGLPPVLKTAVKYPTQSLFRTGMTLAMFILVVFTL
                     TAMNFIQAAMGAAFGATQELSGGHEVRAEAGYADPISDMNAALKEAQGIKKGDIEAVG
                     QVSNLPAEVKQRGTDREPGSLYLQGVDEAYSQSIGYGFQTTEQGYGSAREVWQTLQTE
                     KDTAVISSNLAPARNASTFGPSVESPVKLTGFYASDPNLPDDLYLHVKDPDSGRTKEL
                     RVIAVLESSASFAGQIVTSQKTLEGLAGSPVPPQTYYFDLADGTNAAATADALEKDFA
                     ENGLQTQVTAKVIRDNDATRRIVFLLLRGFMSLGLVVGICALGVIAARSVVERRQQIG
                     MMRALGFQKGQVRLVFLIESSFIALLGLGVGVALGLGFSGTLIDNIREGFPGMEYRVP
                     WGALVLVVVVGYAASVLTTFLPARRASKVYPAEALRYE"
     gene            2287..2541
                     /locus_tag="VNT12_01905"
     CDS             2287..2541
                     /locus_tag="VNT12_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44896.1"
                     /translation="MATRLVELSFGKPSGLVSDIAGPRVYEMKELLRSYLRASHRLIL
                     PPLLPGKAARAIRAGANLALEQAAGHRTWEEFLADRVSQQ"
     gene            2681..3019
                     /locus_tag="VNT12_01910"
     CDS             2681..3019
                     /locus_tag="VNT12_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HWI44897.1"
                     /translation="MRSVRLLTALDRNRRVTAVPFQKSGVPASVGLTLEECKAAAWAI
                     ASDGGRYRGAEAVNAVVAAALGSTLPLLLYSLPGIRQLQDCIYYLVASNRSRLPGDRS
                     YCAQHPAECR"
     gene            3238..5130
                     /locus_tag="VNT12_01915"
     CDS             3238..5130
                     /locus_tag="VNT12_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWI44898.1"
                     /translation="MSSDRTSESASYPPIDDYALIGDCRSAGLVSRDGSLDWLCLPRY
                     DSPSIFAALLDAKNGGRFLVRPLGEYRTERRYLANTNVLETVFRTPTGACVLRDLMSI
                     SSEVDKRTHLTPEHEVLRELEGLEGEVEVEVLYDPRPNYGRVRPLLEERGALGLRCEV
                     DGTSLTLRSEIPLELTDGDRNASGFARIRGGERKYLSLTYSMEAPAVVPLLGEVARGR
                     IERTVHWWQEWANRCTYEGPYRDAVVRSALALKLMTYAPSGALVAAPTTSLPEAIGGV
                     RNWDYRYCWLRDASFTLRALFTLGYREEAEAYVGWLLHATRLTWPELHVLYDVFGEAK
                     LPERELPHLEGYAGSRPVRIGNDAQGQFQLDVYGEVINGAARFLDRGGRVDRDTSRML
                     DGLGRTVCKRWREPDEGIWEGRSGRFHNTHSKVLCWVALDRLIEMHEAGYLDVSVDLF
                     RATRDEIQKEIEAHGYNERIGSYTRTFDGDEMDASLLTLPLYGYIEGTHPQMRSTCSR
                     IHEKLARDGLVYRYETGTDDGLPPGEGAFGICSFWAVECVARGGNVGAADRTFEQLLA
                     HANDVGLFAEEIDPDTGAALGNFPQAFTHIGLINAALTLGEYREGNAPETAERPSNDI
                     RTEDLI"
     gene            5127..5603
                     /locus_tag="VNT12_01920"
     CDS             5127..5603
                     /locus_tag="VNT12_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44899.1"
                     /translation="MNKRGVSLWGFVGTVVLTTLMAGSQGLGLSRINFPFMLGTIFTP
                     NRDRAKLIGFGVHFLNGWIFASVYAAAFESWRRATWWLGGAIGLVHALYVLVAAMPAL
                     PAMHPRMASEQDGPTPTKQLEPPGFMALNYGRRTPITVILAHLLYGTILGAFYRMK"
     gene            complement(5667..6323)
                     /locus_tag="VNT12_01925"
     CDS             complement(5667..6323)
                     /locus_tag="VNT12_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI44900.1"
                     /translation="MATNWDERFFESTRGQIVTLLRRSGRTVEDLARALDLTNNGVRA
                     HLAVLERDGIVRQRGSVRRSGGGGKPAYVYELTEEAEDLFPKAYEPALRRLLDVLSER
                     LGSGELEALLRSVGRRLAEEHSTRADGAHARLESAVEVLNELGGLVELEEHDGGFVIR
                     GYSCPLAGVTPEHPEVCRMAETLIAELAGVPVHERCDRVERPRCCFEVAAAGDTAQKK
                     "
     gene            6452..7840
                     /locus_tag="VNT12_01930"
     CDS             6452..7840
                     /locus_tag="VNT12_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HWI44901.1"
                     /translation="MKAKTLARSVLAGRERLAARAQEVLRQNDMGGWTRAASDLYPHQ
                     WSWDSGFIAIGLAHLDTRRAARELLTLFQHQWKTGKVPHIVFNPEAPPESYFPGPDHW
                     ACAAASADAPPAPPYTSCLCQPPTHAIAAQRIWEAASNGKTRTEATAFLREIYPKLLL
                     WHRYLLTYRDPEESGLVTIYHPWESGTDNSPRWDAALEAVEVGVAPPHPRYDLEHVDD
                     PSQRPTDADYDRYLWLVQLVKRATCEEATIYRSHPFLVKDVLFSAILVAANEALLEIS
                     RLVGASLEDAELILSWISRGRRGLEGRWDPELRLYLDYDLRAAKPLRSRTVAGFSPLV
                     AGGLRRRRLATLLETLRSPSFIDYPGLRRPLLPSTSPEDPGFNPRSYWRGPLWPVIDW
                     LLWWAFVRAGEHQLAERVREASLEQIAEGGFAEYFEPFTGAPLGSKEQSWTAAVVLDW
                     LASGPQRELRAA"
     gene            8293..9015
                     /locus_tag="VNT12_01935"
     CDS             8293..9015
                     /locus_tag="VNT12_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44902.1"
                     /translation="MLARCLCAVSLTLTVLGLFLLVVSRSPVGVPVYAGAPVYDYWLE
                     NTLIAISFSTVGAIITPRLPPQNPIGWIFCSIGVIAGLRLFVSEYAIVTLLAEPRSVP
                     AMLPGGEVLAWISSWLWVSHIGLFVFLALLFPDGRLPSSRWRPFGWLVGVVVVTGTVA
                     VALWPETAAGFDLVNHPLGIEVATDTVNPVETILYALGLVAAASLLVRLRHSVGVKRQ
                     QVKWFAYAVAVLATSAILAYVV"
BASE COUNT         1632 a         2853 c         2803 g         1731 t
ORIGIN      
        1 gacggtggac aggatagact ccatcctctc ggcgaacgat ctcgtggcag atccggtgaa
       61 gaaggacgcc atagatcagg ccgacagccg gggggagacc ttctcgaccc tcttcgtcct
      121 cttcgggcag ttctcggtgg ccgccgggat gctcctgatt ttcctgatct tcgtgatgct
      181 cgccgccgag cgcaagcatg aactgggcat agcccgcgct gtcgggatgc aacgcggggc
      241 cctcgtccga gccttcgcct tcgagggtgc gctctacgct ctcgtggcca gcaccatcgg
      301 cagcgtcgcc ggcgtcgggg tgggctgggt gatggtacgc ttcctgggta gaggtttcac
      361 cggaggcagg gaggacttcc aaatagtctt cgccaccagc ccccagaacg tcatcctcgc
      421 cttctgtatg gggatggtgc tcacgttcat cgtcgtcctg atctcctcct ggcgggtgag
      481 ccgcctgaac gtggtgcgcg cgatacgcga tatcccggaa cctgacaaac gaggccgcac
      541 cgtgatgggt gtgatcgtgg ccgtcctctt gccgcttgcg ggcgcgatag tgttctggca
      601 ggggatcgag acccggacga cggccttcta cctgggcggt ctctcgctcc tgatcatcgg
      661 cgcggcgctc ttggcccgtg tcctgggagt gaccgaccgc gtcgccttca gcgtgtcagg
      721 actcgccctg ctcgcgctgt ggctgacccc tgcctcaatc accacaccgg cggacatggc
      781 gcgcgggccc gagatgtttt tcgtctcagg catcgccctc gtggtggcgg gcgtatggct
      841 cgtggtgttc aacgccgacg tagtcctgcg ggtagtggtc gccctcttcg ggcgaatgag
      901 gggacttcct ccggtcctca agacggcggt gaagtacccg acccagagcc ttttcaggac
      961 cggcatgacg ctggcgatgt tcattctcgt cgtcttcact ctaacggcca tgaacttcat
     1021 ccaggccgcc atgggagcag ccttcggggc tacgcaagag ctctccggtg gccacgaggt
     1081 acgcgccgag gctggctacg ccgaccccat ctccgacatg aacgctgccc taaaggaggc
     1141 acagggcata aagaaaggcg acatcgaggc cgtgggtcag gtatccaacc tgccagcgga
     1201 ggtcaagcag agaggcaccg accgggagcc tggatcgctt tacctgcagg gcgtggatga
     1261 agcatactcg cagagcatcg gatacggctt tcagacgacg gagcagggct acggctctgc
     1321 gcgcgaggtg tggcagacgc tccagaccga gaaggacacg gcggtgatct cctccaacct
     1381 cgctcccgcg cgaaacgctt cgacattcgg cccttcggtc gaatcgcccg tgaagctgac
     1441 cggcttctac gccagtgatc cgaacctgcc cgacgacctc tacctccatg tgaaagaccc
     1501 cgattccgga aggacgaagg aactgcgagt gatcgcggtg ctggaaagct cggcctcctt
     1561 cgccgggcag atcgtgacct cgcagaagac gctcgaaggt ctcgccggaa gtcccgtccc
     1621 gccccagacc tactacttcg acctcgccga tggcacgaac gccgcagcta cggccgatgc
     1681 tctggagaaa gacttcgccg aaaacggcct gcagacgcag gtgacggcca aagtcatccg
     1741 ggacaacgat gcgacaagga ggatcgtctt cctgctcttg cggggcttca tgagcctcgg
     1801 gctcgtggtc ggcatctgcg ccctgggtgt catcgctgcc cgctcggtgg tggagaggcg
     1861 gcagcagatc gggatgatga gagctctcgg attccagaag ggtcaggtac gcttggtctt
     1921 cctgatcgag tcctccttca tcgcgttgct cgggctgggc gtcggggttg ctctagggct
     1981 cgggttctcc ggcacgctca tagacaacat ccgcgagggc ttcccgggca tggagtacag
     2041 ggtgccttgg ggcgcgttgg tgctcgtcgt cgtggtcggc tacgccgcct ctgtgcttac
     2101 caccttcctg cccgcccgcc gggcctcaaa ggtctacccg gctgaggcgt tgcgctacga
     2161 gtagcacgcc gaggacggtc cgtctcacgg accgtttctt acggtccgtg tgtggctagt
     2221 cagaggcggc catccccgcc acgctcgcct cctccatctc ttcgtttgag tagcgacggc
     2281 ggtgaagtgg cgacccggct cgtcgaactc tccttcggca agccgtccgg cctcgtgtcc
     2341 gacatagccg ggccacgcgt gtacgagatg aaagagcttc tccgcagcta cctgcgggcc
     2401 agtcaccgcc tcatcttgcc gcccctgttg ccaggcaagg cggcccgcgc gatccgggcc
     2461 ggtgctaacc tggctctcga gcaggccgcg ggccacagga cctgggagga gttcctcgcg
     2521 gaccgggtga gtcagcaata ggtttgaggg ctccacagga ccggatggct gagatgaggc
     2581 caaggtccgg cgagtgtgcc ggtgtacaat caggctgata gctgtggctg agcggccccc
     2641 acaactcact cttgtattcg acggtacctg agggttctgc atgcggtcgg tgcgccttct
     2701 tacggcgctc gatcgcaacc gtcgcgtgac ggcggtgccc ttccagaaga gtggcgttcc
     2761 cgcctcggta gggctcactc tcgaagagtg caaggcggcc gcgtgggcga tagcatccga
     2821 cggcggccgc taccgaggcg ccgaggcagt aaacgcggtg gtggctgccg ccctcggaag
     2881 cacactcccg ctactgctgt actccttgcc aggtataagg caactgcagg actgcatcta
     2941 ttaccttgtc gcctccaacc gcagtcgttt gcccggcgac cgatcctact gcgcccaaca
     3001 tcccgccgag tgcagatagt actctgagga gatcgtcagg aactgcaagc tcaatataat
     3061 aaatctaata aatacttgat ttattataga accggtgtta ccgttccggc catgcggcac
     3121 gtcaagcggc acaagcgaaa cagaggagac atctggttta aggtatggag gccgcgcggc
     3181 gttgtgcgga cgatgaaggc aggcaactga caaaaggtgc tagaacttag ttgacacatg
     3241 tcgtctgatc gtacatcaga aagcgcaagc tacccaccca tagatgacta tgccctcatc
     3301 ggcgattgcc ggagcgctgg gctagtatca cgcgacggct cgcttgactg gctttgtcta
     3361 ccccgttacg atagtccgtc catcttcgct gccttactgg acgcgaagaa cggtgggcgg
     3421 ttccttgtgc ggccgctcgg cgagtaccgt acggagcgcc gctacctggc gaacaccaac
     3481 gtgctcgaaa ccgtcttccg cactcctact ggagcgtgcg tactgcgtga tctcatgtcg
     3541 atctcgtccg aagttgacaa gcgaacgcac ctgactcccg agcacgaggt attgcgcgag
     3601 ttagaagggt tagagggaga ggtcgaggtc gaggtcctct acgacccccg ccctaattat
     3661 ggtcgggtcc gcccgttgct cgaagagcgc ggagcgcttg gtctgcggtg tgaggtcgat
     3721 ggaacgtccc tgacactccg cagcgagatt cctctggagt tgaccgacgg tgatcgtaac
     3781 gccagtgggt ttgcccgaat ccggggcgga gagcgcaagt atctttccct cacctacagt
     3841 atggaagccc ctgctgtggt tccccttctc ggggaggtcg cgcgaggaag aatcgagcga
     3901 acggttcact ggtggcagga gtgggcgaac cgttgtacat acgagggacc ttaccgcgac
     3961 gcggtcgtgc gcagcgcctt ggcattgaag ctgatgacct acgcgccctc cggcgctctc
     4021 gtcgcggcgc cgaccacctc cctccccgag gccataggag gcgtacggaa ctgggactac
     4081 cgctactgct ggctgcgcga cgcgtctttc acgttgcgcg ccctgttcac ccttggctac
     4141 cgggaggagg cggaggccta tgtcggctgg ttgttacacg ccacgcggct aacctggccc
     4201 gagctccacg tactctacga cgtcttcgga gaggcgaagt tgcccgaacg ggaactaccg
     4261 catctggaag gctacgcggg gtctcgcccg gtgcggatcg gcaacgacgc ccagggccag
     4321 ttccagctcg acgtctacgg agaggtcatc aacggcgccg cccggttcct ggaccgcggc
     4381 gggcgcgtcg accgcgacac gagccggatg ctcgacggtc tcggtcgcac ggtgtgcaag
     4441 cgatggcgcg agcctgacga ggggatctgg gaggggcgct caggccgatt ccataacacc
     4501 cactcgaagg tcttgtgctg ggtcgcgctc gatcgactca tcgagatgca cgaagccggg
     4561 tacctggacg tctcagtcga cctcttccgc gccactcggg acgagatcca gaaggagatc
     4621 gaggcgcacg ggtacaacga gcggataggc agctacacgc gcaccttcga cggcgacgag
     4681 atggatgcaa gtctcctgac cctaccacta tacggataca tcgaaggtac acacccgcag
     4741 atgcgctcca cttgctcccg catacatgag aagctggccc gcgacgggct agtctatcgc
     4801 tacgagacgg gtaccgatga cgggctcccg cccggagaag gcgctttcgg catctgcagc
     4861 ttctgggcgg tggaatgtgt ggcccgtggc ggcaacgtcg gagcagccga ccggaccttt
     4921 gagcaactac tcgctcacgc gaacgacgtg ggcctcttcg cagaagagat agacccagat
     4981 accggcgcgg cactcgggaa cttcccgcag gcgttcacac acatcggtct catcaacgcg
     5041 gcgctcacgc tcggcgaata ccgtgaaggg aacgcacctg agactgctga gcgtccatcg
     5101 aacgatatac gaacggagga tctgatatga acaagcgtgg cgtctcgctt tgggggtttg
     5161 taggtaccgt cgtcctgacg accctgatgg ccggtagcca ggggctcggt ctgagcagga
     5221 taaatttccc ctttatgctg gggaccattt tcacaccaaa ccgggaccgg gcgaagctga
     5281 taggcttcgg agtacacttc ttgaacggct ggatcttcgc cagcgtttac gccgcggcct
     5341 tcgagagctg gcgtcgggca acctggtggc taggaggagc catcggcctg gtgcatgcgc
     5401 tctacgtgct ggtcgctgcc atgccggccc tgcctgcaat gcacccgcgg atggcgagcg
     5461 agcaggacgg tcctacgccg accaagcagc tcgaaccacc tggtttcatg gccctcaact
     5521 acggccggcg cacgccgatt acggtgatcc tggctcacct cctctacggc accatcctag
     5581 gcgccttcta ccgtatgaag taagtccaca cggccggcgc cgcagggatc tctctgccgt
     5641 accgctactc gttgtgatgg cctgtgttac ttcttctgcg cggtgtcgcc agcagccgct
     5701 acctcgaagc agcagcgagg cctctcgaca cggtcgcaac gctcatggac ggggacgcct
     5761 gccagctcgg cgatcagcgt ctccgccatg cggcaaacct ccgggtgctc cggcgtcacg
     5821 ccggccagtg ggcagctata accgcggatg acgaagccgc catcgtgctc ctcgagttcg
     5881 acgaggccac ccagctcgtt gagtacctca accgccgact cgagccgcgc gtgcgcgcca
     5941 tctgcccgcg tggaatgctc ttcggctagg cggcgaccca ccgaccttag tagcgcctcc
     6001 aattccccag atccgagccg ctcggatagg acatccagga gccggcgaag cgcgggttcg
     6061 taagccttcg ggaagaggtc ttcggcttcc tccgtcagct cgtagacgta agcaggcttt
     6121 ccgccgccac cgctgcgacg caccgaacct cgctgtcgca cgatgccgtc gcgctcaaga
     6181 acggcgagat gtgcgcggac cccattgttc gtcagatcga gcgcccgggc gaggtcctcg
     6241 acggtgcgcc ccgagcgcct caggagcgtg acgatctgac cccgcgtgct ctcgaagaac
     6301 ctctcgtccc agttcgtagc caatgtactc cctacaccgc ttcactttca gtatagcaag
     6361 taaaccaaat tcttgtaata aatcaagttt atacttgaca tattgaagaa acggtgtata
     6421 gactgcatcg agactcaaag gagatgaggc aatgaaggca aagaccttag caagaagcgt
     6481 actcgcgggg cgcgaaagat tggccgcgcg agcacaagaa gtccttcgcc agaacgacat
     6541 gggggggtgg acgagggcgg cctcggacct ctacccccac cagtggagct gggatagcgg
     6601 cttcatcgcc atagggctgg cccacctcga cacacgccgc gccgcccggg agctgctcac
     6661 gcttttccaa catcagtgga agaccggcaa ggtgccccac atcgtcttca accccgaggc
     6721 cccgccggag agctacttcc caggccccga tcactgggcc tgcgccgcgg cttctgccga
     6781 cgctccgcct gcgccgccct acacaagctg cctgtgccaa ccaccaaccc atgccatagc
     6841 cgctcagagg atctgggaag ccgccagcaa cgggaagacg cgcacggaag ctaccgcctt
     6901 cttgcgcgag atctacccta agctcctcct ctggcaccgc tacctcctta cctaccggga
     6961 tccggaggag tcggggctgg taacgatcta ccacccgtgg gagagcggca ctgacaactc
     7021 cccgaggtgg gatgcggccc tcgaggcggt ggaggtaggc gtggcgccgc cccaccctcg
     7081 ctacgatctt gagcacgtcg acgatccttc acagaggccc accgacgcgg attacgatcg
     7141 ctatctctgg ctcgtgcagc tggtaaagag ggccacctgc gaggaggcca ccatctaccg
     7201 ctcacaccca ttcctagtga aggacgtact cttttccgcc atcctcgttg ctgccaacga
     7261 ggcgctgctg gagatctccc ggctggtagg ggcctcgctc gaggacgcgg aactcatctt
     7321 gagctggatc tcccgcgggc gccgcgggct cgaaggccgc tgggatcccg agctccggct
     7381 ctacctcgac tacgacctgc gcgccgccaa acctctgagg tcgcgcacgg tggccggctt
     7441 ctccccgctg gtagccggag gcctgagacg ccggcgcctc gcgaccctcc tggagaccct
     7501 cagatcgcct tcttttattg actacccggg gttacgacgg cctctgctac cgagcacgag
     7561 cccggaggat cccgggttca accctcgcag ctactggcgg gggccactct ggccggtgat
     7621 cgactggctt ttgtggtggg cgtttgtgcg ggccggagaa caccagctcg ccgaacgcgt
     7681 gcgcgaggca tccctcgagc agatcgctga aggaggcttt gccgagtact tcgagccctt
     7741 caccggcgcg ccgctcgggt ccaaagagca gtcctggacc gctgccgtgg tcctggattg
     7801 gctggcgagt gggccgcagc gggagctgcg agcggcgtag acggtggcct aaactcggat
     7861 ggaggagttg ggcagcaact atcgcccgtc ttcggcagaa cccgccgggc tggttggctt
     7921 gctcaagagc atcccgagac cggcgcgatc gtctacgagg ctctgttccc ggccctcgcc
     7981 gaagacgcga atacacctgt ggtcagtgcc gacggtaagg tcatatccga gtgcctagaa
     8041 aaggagtgtt ctctgaagat gcgatgagaa ggccgacccc ctaggcagtg acaaaatgtg
     8101 ccttctcata gaagttgctc tgcacttcag tgcaaaacca cccaatttgg ggatgcgacc
     8161 gatcgtccga aatgtcatca ttgtttccta gaacgatcgg tggggcagta ccatgggctc
     8221 actatgacaa cgagcgctgg agacgagagc ttcgagcgag gagctgccag gctgtaccct
     8281 cgtgcgacga ccttgctggc caggtgcctg tgcgccgtct ctttgacgct gacggtactc
     8341 ggcctcttcc tcctggtcgt gagccggtcc cctgtgggag ttcccgtcta cgcgggcgcc
     8401 cccgtatacg actactggct cgaaaacacc ttaatagcaa ttagcttttc gacggtaggg
     8461 gccataatca ctccccgact tccccctcag aaccctatag gctggatctt ctgctccata
     8521 ggcgtgattg cagggttgcg cctctttgtc tccgagtatg ccatcgtgac gttgctggcg
     8581 gagcccagat cggtgccggc catgctgccg ggcggagagg ttttggcttg gatctcctca
     8641 tggctgtggg tttcgcacat tggtctcttt gtgttcctgg ctttgctgtt ccctgatggc
     8701 agacttcctt catcgcgttg gcgccccttt gggtggcttg ttggtgtggt ggtcgtgaca
     8761 ggtacggttg ctgtcgcgct ctggcccgag acggccgcgg gattcgatct cgtcaaccac
     8821 cccctcggca tagaagtcgc aacagacaca gtgaacccgg tggaaactat cctgtacgcc
     8881 ctcgggctcg tagcagcggc ctcgctgttg gtacggctgc gccactctgt gggggtgaag
     8941 cgccagcagg taaagtggtt cgcctacgcc gtggcggtgc tggctacaag cgccatcctc
     9001 gcctatgtag tctagatcg
//
LOCUS       DATMJA010000084         3004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_189225, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000084 DATMJA010000000
VERSION     DATMJA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189225"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1617
                     /locus_tag="VNT12_01940"
     CDS             190..1617
                     /locus_tag="VNT12_01940"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HWI44903.1"
                     /translation="MLSREYTGPLGEKGASPGARWLLADRVSRALSDARLERIADPVE
                     RIARLALPAGRVDPFRWLSEQRMFPKVYWSGREDRAGVAAVGIADLREVGVSEGEGSL
                     SKVLASLPDSGISGARYYGGARFDPLGQPDEEWAAFSAYRFVLPRFELHAGETETMLV
                     CNLVLPRDMDDASKIVQEIEDLSLPEGTSGALLPAPMFREDSPNFEGWRENVKRALSA
                     LSEGRLGKVVLARRAEFGFCRDLDPTLLLESLKAATPGCFHFYVEPEDGIAFLGASPE
                     RLFRREGRSVESEAVAGTRPRGVSSAEDEGLRDDLLHSAKDRSEHTYVSKGIREALEP
                     LCEVLEIEDHVSEMMLARGRHLRSKVRGTLEDDVTDAALLDAMHPTPAVGGYPRSEAL
                     EQIHALEPFDRGWYAGPVGWIGQEASEFAVGIRSGLARGRTLALFSGAGIVAGSFPEE
                     EWAEIEQKIGDFTGIFGIERGHAAS"
     gene            1592..>3004
                     /gene="menD"
                     /locus_tag="VNT12_01945"
     CDS             1592..>3004
                     /gene="menD"
                     /locus_tag="VNT12_01945"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HWI44904.1"
                     /translation="MNGDTPRANRLWAYLIVEELLRCGIDFFCVAPGSRSTPLVAALA
                     ANEKARSLIHFDERGTAFAALGYARATGRPAAWITTSGTAVANGLPAVVEAATDGVPM
                     VLLTADRPPELRQTGANQTIDQPDIFGDYVRWRFDLPAPDPGIDPAMVLTTVDQAAYR
                     ASRTPQGPVHLNLMFREPLLPDPGDEPVLSGPSSWVQSDEPYTRYAATKPGVDEMEIH
                     RLWEQLRPVKQGLVVAGRLASRKQGDAVLRLADTLGWPLLPDTGSQVRLGTEGLAAYY
                     DALLAGDSFAGAHAPEAVVHVGGRALSKRLEQFLAHSRPDPHVVVRENPFRLDPTHRV
                     THSVEANILDFCSALVRAATEDPPAMDSSWTAGWREASEEVDRYLDGISSGEPNEPLV
                     ARVLSQNVPPDHSLVVASSMPVRDLDTYAAAGGAPVPVAANRGASGIDGTVATAAGFA
                     RGVGRPVTLLIGDLALLHDLN"
BASE COUNT          525 a          949 c          986 g          544 t
ORIGIN      
        1 ctcacccccg aacccgagag ccgcatactg cccggcgaga cgacttacga ggccgcctac
       61 gaacggtaca ccgaactcta ccccaggctc tacggttgag gtgcctggcc tgcgactgtg
      121 agagaagccg ctaaaacgct agatactcct tgctacacta caccctgaga tgatgaggtg
      181 gcatagatct tgttgagcag ggaatacact ggcccccttg gggagaaggg cgcgtcacca
      241 ggtgcccgct ggctgctggc cgaccgggtg agccgtgcgc tctcagatgc aagactggaa
      301 aggatcgcgg acccggtcga gcgaatcgca cgtcttgcct tacccgcggg ccgggtagac
      361 cctttcaggt ggctctcaga gcagcggatg ttcccgaagg tctactggtc gggacgtgaa
      421 gaccgagccg gggtcgcggc cgtggggatt gcggacctgc gggaggtggg cgtctctgag
      481 ggtgagggct ctctgtccaa ggtgctcgcg tcgctacccg actcagggat ttcaggggcg
      541 cgctattacg gcggagcgcg cttcgatccg cttggccaac ccgatgagga atgggctgcg
      601 ttcagcgctt accggttcgt cctgccgcgc ttcgagctgc acgcggggga gacggagacg
      661 atgctggtct gcaacctggt gctccccagg gatatggatg acgcctcgaa gatagtgcag
      721 gagatcgaag acctctcgtt gcctgagggt acgtccggag ctctgttgcc ggcaccgatg
      781 ttccgtgagg atagccccaa ctttgagggt tggagagaga atgtcaagcg ggctctttcg
      841 gcgttgtcgg aaggtcgtct ggggaaggtc gtgttagccc gcagggctga gttcggtttt
      901 tgtagggacc tggatcccac gctgctattg gagagcctga aggcggctac gcctggctgc
      961 ttccatttct acgtcgagcc cgaggatggg atagcatttc tcggggcctc tcccgagcgt
     1021 ctcttccgcc gtgaaggacg ctcggtggag agcgaggctg tggcggggac caggccccgt
     1081 ggagtatctt cggcggagga cgaagggctg cgcgacgacc tgttgcatag cgctaaagac
     1141 cgctccgagc acacttacgt gagcaaagga ataagggagg cgctggagcc gctctgtgaa
     1201 gtactggaga tcgaggatca cgtctctgag atgatgctgg cgcggggaag gcacctcagg
     1261 tcgaaggtcc gtgggacgct cgaggatgat gtgaccgacg ccgcactgct cgacgcaatg
     1321 caccctacgc ccgccgttgg cgggtatccg cgaagtgaag cgctggagca gatacacgcc
     1381 ctcgaaccct tcgaccgggg ctggtacgct ggccctgtcg gatggatcgg gcaagaagcc
     1441 tccgagttcg ccgttggtat ccgctcgggg ctggcgcggg gaaggacgct ggccctcttt
     1501 tcgggcgccg ggatcgtcgc gggctccttt cctgaggagg agtgggccga gatcgagcag
     1561 aagatcggcg acttcacagg gatattcggg attgaacggg gacacgccgc gagctaatcg
     1621 cctctgggcg tacctgatcg tcgaagagct tctccgctgc ggcatagact ttttctgcgt
     1681 cgcccctggc tcgcgctcga cgcccctggt cgcggcgctg gccgcgaacg agaaggcccg
     1741 tagcctcatc cacttcgacg agcgtgggac cgccttcgca gccctcggct acgctcgcgc
     1801 tacaggacgt cctgcggcct ggatcacgac ctcgggcacc gcggtcgcta acggtcttcc
     1861 ggccgtcgtg gaggccgcga ctgacggcgt gcccatggtc ctgctcaccg ctgaccgacc
     1921 tcccgaactc cgccagaccg gcgccaacca gaccatagac cagcctgaca tcttcggcga
     1981 ttacgtccgc tggcgcttcg atctgccggc ccccgatccc ggcatcgatc cggccatggt
     2041 cctgacgact gtggatcagg ccgcctaccg cgcgagccga acgccacagg gccccgtcca
     2101 cctgaacctg atgttccgcg agcctttact ccccgatccg ggggacgagc ccgtactatc
     2161 cggaccatcc tcttgggtgc agagcgacga accgtacacc cgctacgccg cgacgaaacc
     2221 cggcgtagac gagatggaga tccaccgcct ctgggaacag cttcgccccg tgaaacaggg
     2281 tctcgtcgtc gcaggacgac tggcctcccg caagcagggc gatgcggttt tgcggctcgc
     2341 cgacactcta ggctggccgt tactaccgga cacgggttcc caggtccgtc tcggtacaga
     2401 aggcctcgcc gcctactatg atgcgctact ggctggggac tcattcgcgg gggcacacgc
     2461 acccgaggct gtagtccacg tcggaggaag agctctttcc aagcgcctgg agcagtttct
     2521 tgcccacagc cgcccagacc ctcacgtcgt cgtacgcgag aaccctttca ggctcgatcc
     2581 aacccaccgc gttacccaca gcgtcgaggc taacatcctt gacttctgct ccgcgctggt
     2641 acgggccgct accgaagatc caccggcgat ggactcttca tggacggcgg ggtggcggga
     2701 agcatcggag gaggtggatc gctatctcga tggaatctcc tccggagaac cgaacgagcc
     2761 gcttgtggcc cgggtcctat cgcaaaacgt acccccggac catagtctcg tcgtcgcgag
     2821 cagcatgccc gtgcgggacc tggataccta cgctgcggcc ggcggcgcgc ccgtccctgt
     2881 cgccgccaac aggggcgcga gcggcatcga tggcactgtc gccacggcgg caggcttcgc
     2941 gaggggcgtc ggacggcctg ttacgctcct catcggagat ctcgcgctgt tgcacgatct
     3001 caac
//
LOCUS       DATMJA010000085         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_603780, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000085 DATMJA010000000
VERSION     DATMJA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603780"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="gatB"
                     /locus_tag="VNT12_01950"
     CDS             <1..1176
                     /gene="gatB"
                     /locus_tag="VNT12_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWI44905.1"
                     /translation="GYQISQFDEPICTGGHIDVPTPEGKIRVGITRLHLEEDAAKNVH
                     VGTTGRMHGSVGSLVDFNRGGTPLMEIVTEPDIPSPEAARATANQLKDILRAVGVSEA
                     DMEKGQLRCDANVSIRNPDGTFGTKTELKNMNSFRFVERGLARELERQREILENGGRV
                     EQTTLHYDPETDEVHELRSKEYAHDYRYFPEPDLLPLEFSPAYVREIEDRLPELPDHM
                     LARFIEQYDLSEYDAGVLTSDRNLASFYEAVAAEVDPKQASNWISGDLRALLNETGTD
                     ISDSRVTPEHIKELIELVAAGKVSRSAARTALDTVFETGDSPSAIVEREGLASVGSDE
                     LSGLVDDVISANPEEAERVRGGDKKVLGFLIGQIMKATRGNADGGRVRELLMKKLGS"
     gene            1425..>2474
                     /locus_tag="VNT12_01955"
     CDS             1425..>2474
                     /locus_tag="VNT12_01955"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWI44906.1"
                     /translation="MAREWLADLVEISCSGMDLDGLLLGASSAEELAGMLVASLRLDL
                     PTVYTPPASTPLCAALVALGLAPRAGGPATTVVSLTERFGPRPRGLVDNFSLANALRA
                     GVAAGGGPVILIHLAAIAREAGVAGFGQMMRVLAAETPEADPEWLREYGIPGLLSSLG
                     DTLHDVPTVAGNLKENLPSVPPAPEERSRFVFIRARASGAEAVCQVRKGVTEPVGECR
                     VFGSEEVAVSSVLRGEVGEGALLVVGGCGPRGGPGLLRLDALGQSLKESGLEVPVLTD
                     GLAPEDAEGAWISLFTPEAADGGVLSLLRDGDTLRIDLAEGRIRTGAREFTSREPGEF
                     PNSAGQAYTARYARNA"
BASE COUNT          457 a          817 c          781 g          419 t
ORIGIN      
        1 gggtaccaga tctcccagtt cgacgagcct atctgtacag gaggccacat cgacgtccct
       61 acccctgaag gcaagatccg ggtggggatc acgcggcttc acctcgagga ggacgcggcg
      121 aagaacgtac acgtcggtac cacgggccgc atgcacgggt ccgtcggctc cctggtcgac
      181 ttcaaccgcg ggggcacgcc cctgatggag atcgtcaccg aacccgacat cccatcgccc
      241 gaagcggcga gggctacagc caaccagctc aaggacatcc tgagggcggt cggcgtttcg
      301 gaagccgaca tggagaaggg gcaactccgc tgcgacgcca acgtctccat acgcaacccc
      361 gacggaactt tcggcaccaa gaccgagctc aagaacatga actcctttcg ctttgtcgag
      421 cggggcctcg cgcgcgagct ggagcgacag cgtgagatcc tggagaacgg gggccgtgtc
      481 gagcagacca cgctccacta cgacccggag accgacgagg tgcatgagct ccgcagcaag
      541 gagtacgccc acgattatcg ctacttcccc gagcctgatc tcctgccgct tgagttctcg
      601 ccggcctacg tcagagagat agaggaccgg ctccccgagc tgcccgacca catgctcgcc
      661 cgcttcatcg agcagtacga cctctctgag tacgacgccg gggtacttac gtcggataga
      721 aacctcgcat cgttctacga agcggtcgca gcggaggtgg atcccaaaca ggcttctaac
      781 tggatctccg gtgatctgcg cgcgctcctg aacgagaccg gcacagatat ctctgactcc
      841 agggttacgc cggagcacat aaaagagctg atcgaactcg tcgcagcagg caaggtgtcg
      901 cgctccgcag caagaacggc cctcgacaca gtcttcgaga ccggcgactc cccatcagcc
      961 atcgtcgagc gcgagggcct cgcttctgta ggctccgatg agctctccgg cctcgtagac
     1021 gacgtcatct ctgccaaccc ggaagaggcc gagcgtgtcc gaggcggcga caagaaggtc
     1081 ttaggttttc tcatcggcca gatcatgaaa gccacccgcg gcaacgccga cggcgggagg
     1141 gtccgcgagc tcctgatgaa gaagctgggc tcctagagtt cgaggcttca ggttacaggt
     1201 atcaggaatt ctctacaaac aaagtggagg accagaacgg gatatgtgcc accctggtgg
     1261 ttcaaccagt ccgcaaaatg ctatagagga ctgcggtcct gaacgccccg cgtatcggcg
     1321 tccctttcct cgaaccctcg ggcctccctg acgccgtcta cgatgcagga ggagagccag
     1381 taccccttcg cctgccgccc tcagtacccg aagggggcgt cgcgttggcg cgggagtggc
     1441 tcgctgacct ggtagagatc tcctgctccg gcatggacct cgacggtcta ctgctaggtg
     1501 cctcaagcgc cgaagagctc gccggaatgc tcgtcgcctc tctgcgcctg gatctcccta
     1561 cggtctacac accgccggcc agcacgcctc tctgcgccgc cctcgtggcg cttggccttg
     1621 cgccacgggc cggaggccct gcaactacgg ttgtctcact caccgaacgt ttcgggccac
     1681 gccctcgcgg gctggtcgat aatttctcgc tcgccaacgc gctgcgggcc ggcgttgcgg
     1741 cgggcggagg gcccgtaatc ctcatccacc tcgcggcgat cgcccgtgag gccggtgtcg
     1801 ccggatttgg ccagatgatg cgggttctcg ctgcggagac ccccgaagcc gaccccgagt
     1861 ggttgcggga gtacgggata ccgggcctcc tctcttccct gggcgacaca ctccacgacg
     1921 ttcctaccgt cgctggcaac ctgaaagaga atctgccgtc cgtgcctcct gcgccggagg
     1981 agcgctctcg cttcgtcttc atcagggcaa gggcgtcggg cgccgaggcc gtctgccagg
     2041 tccgcaaggg cgtgaccgag ccggtgggag agtgccgcgt cttcggctcc gaagaagtgg
     2101 ccgtctcgag cgtgcttcgt ggcgaggttg gagaaggagc gttgctcgtg gtgggaggtt
     2161 gcggaccgcg cggcggcccg ggactcctcc ggctagacgc gctcgggcag tcactaaagg
     2221 agtccggact cgaggtgcct gtgctgaccg acgggctcgc gccagaagac gctgagggag
     2281 cctggatctc cctgttcacc cccgaagcgg cggatggcgg tgtcctcagc ctactccgtg
     2341 atggtgatac cctgaggata gatctcgccg agggccgcat ccgcaccggg gccagggagt
     2401 tcactagcag ggagcccggc gagttcccca acagcgcagg ccaagcttac accgcccgtt
     2461 acgcgcggaa cgcg
//
LOCUS       DATMJA010000086         3773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_130223, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000086 DATMJA010000000
VERSION     DATMJA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130223"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VNT12_01960"
     CDS             <1..773
                     /locus_tag="VNT12_01960"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWI44907.1"
                     /translation="TAEVLNTQYPNHATELASFAGDRLVVAAGGDGTVNEVLNGLSLE
                     ATLGVLPLGTANVLARELGLPLDPVEACRRILKGERSRVDFGVATDDDGAERRFACMA
                     GIGFDAKVISAVTPRLKRYLRRLAFQLTAFRVLFGNRFPTLEILLGEEIHTARFAIIA
                     NGQYYGGDYRVSGPGLLTSGEFETILVERVSDLLRPDVFAGIMARRPLNRAMTSFTST
                     EVHARAPGAEVPVQLDGELWGGLPMSFRIEPGALNVIH"
     gene            complement(774..1763)
                     /locus_tag="VNT12_01965"
     CDS             complement(774..1763)
                     /locus_tag="VNT12_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWI44908.1"
                     /translation="MILDVPGEPLRVAEVPVPEPGEGQLLLRVHCCAVCRTDLHVVDG
                     ELPHPRLPLIPGHQIVGTVEKIGGQERSFAVGDRVGVPWLGWTDGECRYCLSGRENLC
                     ENARFTGYQIDGGYAEYAVADGRFCFPIPEGFPDLQAAPLLCAGLIGHRSLRFAGDAE
                     KLGLYGFGASGHIVAQVAAHEGRRIFAFTSPHDEEAQEFARELGAEWAGSSEEAPPEE
                     LDAAIIFAPVGALVPAALRAIARGGTVVCAGIHMSDIPSFPYEILWGERSVRSVANLT
                     RQDGLDFLALAPEVPVRTEVVPFPLEEANQALDALRGGRVRGAAVLVAAVAGG"
     gene            complement(1823..3658)
                     /gene="glmS"
                     /locus_tag="VNT12_01970"
     CDS             complement(1823..3658)
                     /gene="glmS"
                     /locus_tag="VNT12_01970"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWI44909.1"
                     /translation="MCGIVGYAGKERCVEVLMEGLGHLEYRGYDSAGLALQKEGQIER
                     ITEIGQLENLTRALAGRNGDLSQVRTGVGHTRWATHGRSSEENAHPHLGGDGSVAIVH
                     NGIIENFSELKEELEGRGVIFRSETDTEVIAHLIAERVEVGETLRIALAGVFQRLQGS
                     FAVAAVSVDEPGKVVAARHQSPLVVGLGEGENFLASAVQALLGETHEFLVVENGEIVE
                     VAASSVEVFTSSGEPVEREAFEVDWDAEAVELGGYEDYMLKEIHEQPAALGATLLGRI
                     DADGVVDLSEVEIDLSEIDRVVIVACGTAYHAGLLGKHAIEKLARTPVEVVIASEYRY
                     ADPIGDDHTLVVAISQSGETTDTLAAVQAARSFGAKVLAVTNTRGSLITREADAVLIT
                     KAGPEICVASTKTFVAQVAVLDLLALALAGIRGAIPERELREIGRGLRLAPEKVEETL
                     GLLENRVEEAIEIFEDARCSLFLGRGISYPAALEGALKLKEISYLPSEGYPAGEMKHG
                     PIALVDEHCPVIAILGEGLIREKTISNVEETLARGASVIAVARQGDKGAERMARLLLP
                     VPEAPEILAPFICSVPLQLLAYHVAKDRGLDVDKPRNLAKSVTVE"
BASE COUNT          677 a         1285 c         1159 g          652 t
ORIGIN      
        1 tgacggcgga agtactcaac acccagtacc ccaaccacgc caccgagctt gcatcctttg
       61 cgggggaccg tctggtcgtg gccgctgggg gcgacggcac ggtcaacgaa gtgttgaacg
      121 ggctctcgct agaggccacg cttggcgtgc tgccgctagg cacggcgaac gtgctggcgc
      181 gggagctcgg gctgccactc gatccggtag aggcctgccg gcgcatactg aaaggcgaac
      241 gctccagggt ggacttcggc gtcgccacag atgatgatgg agccgagcgc cgcttcgcct
      301 gtatggccgg cataggcttc gacgcgaaag tgataagcgc cgtgacgccg cgcctgaagc
      361 gctacctgcg taggttggct ttccagctaa cggcattcag ggtgctcttc gggaacaggt
      421 ttcccacact ggagatactc ctcggcgagg agatccacac tgcgaggttc gccataatcg
      481 ccaacggcca gtactacggc ggcgactaca gggtgtcagg gccaggcttg ctcacgagcg
      541 gcgagttcga gaccatcctg gtcgagcgcg tgagcgacct cctgcgacct gacgtcttcg
      601 ccgggatcat ggcgcgaagg cccctcaacc gcgcgatgac gtcttttacc tcgacggagg
      661 ttcacgccag ggccccaggc gcagaggtgc ccgtacaact cgacggtgag ctctggggcg
      721 gactgcccat gtccttccgc atcgaaccag gcgccctgaa cgtaatccat tagctacccc
      781 ccggccacag ccgcgaccag cactgcagcc ccccgcaccc taccgccgcg cagggcatcg
      841 agggcctgat tggcctcctc caaggggaaa ggcacaacct ccgttcgaac aggcacctca
      901 ggggcgagag cgaggaagtc caggccgtcc tggcgcgtga ggttggccac cgagcgcacc
      961 gagcgctctc cccagaggat ctcatagggg aatgacggga tgtcgctcat gtgtatgccc
     1021 gcgcagacca cggtcccgcc cctggcgatg gccctgaggg cagcagggac gagggcgccg
     1081 accggcgcga agatgatcgc cgcgtccagt tcctcgggtg gcgcctcctc agagctcccg
     1141 gcccattcgg cgccaagctc cctggcaaac tcctgcgcct cctcgtcatg cgggctggtg
     1201 aaggcgaaga tacgccggcc ctcgtgcgct gcgacctggg ccacgatgtg gcccgaggcc
     1261 ccgaagccgt agagaccgag cttctcggca tcgccagcga agcgcaacga gcggtgcccg
     1321 atcaggcctg cgcacaacag cggcgcagcc tgcaggtcgg ggaatccctc ggggatagga
     1381 aagcagaaac gaccgtcggc tacggcgtac tccgcatagc cgccgtcgat ctggtacccg
     1441 gtgaatcggg cgttctcgca caggttctcg cgcccggaga ggcagtagcg gcactcaccg
     1501 tcggtccacc caagccaagg caccccgacc ctatccccca ccgcgaagct gcgttcttgc
     1561 ccgccgatct tctccacggt accgactatc tggtggcctg ggatcagggg caatctggga
     1621 tggggcaact ccccgtcaac gacgtgcaga tcggtcctgc atacggcgca gcagtgtacg
     1681 cgcaggagga gctgcccctc tcctggctcg ggcacaggca cctccgcgac cctcaacggc
     1741 tcgccgggta cgtccaggat catcgctcgc acaggcgtat cctcctaaat taggagcagg
     1801 aacggcctcg ggaacacgct cgctactcta ccgtgacgct cttggcgagg ttgcgtggct
     1861 tgtccacgtc gaggccccgg tccttggcaa cgtggtatgc gaggagctgc aacggcacgc
     1921 tacagatgaa gggagccagg atctccggcg cctcgggaac agggagcagt aggcgcgcca
     1981 tcctctctgc gcctttgtcc ccctggcggg cgacggcgat cacactggcg cccctggcga
     2041 gggtctcctc gacgttggag atggtcttct cgcgaatgag gccttctccg agaatggcta
     2101 tgacgggaca atgttcgtcc accagtgcaa taggaccgtg cttcatctcg cccgccggat
     2161 agccctcaga gggtaggtag gagatctcct tgagcttcaa tgccccttcg agcgccgcgg
     2221 gatacgagat accacgcccc aggaagagcg agcaccgcgc atcctcgaag atttcgatgg
     2281 cctcctcgac ccgattctcg agcaaaccga gcgtctcctc gaccttctcc ggcgccagcc
     2341 tgaggccgcg accgatctcc cgcaattctc tctcggggat ggctccgcga atgccggcga
     2401 gcgccagtgc cagcaggtcg agtacggcga cctgggcgac gaaggtcttg gtagaggcaa
     2461 cacagatctc aggtcccgcc ttggtgatca agaccgcgtc ggcctcacgt gtgatgagcg
     2521 accctctggt attggtaacc gcaagcacct tggccccgaa gctccgcgcc gcctgaacgg
     2581 ccgccagagt atccgtggtc tccccgctct ggcttatggc aacgaccagc gtgtggtcgt
     2641 ctccaatagg gtccgcgtag cgatactcgc tggcgatcac gacctcgacg ggtgtcctgg
     2701 cgagcttctc tatggcgtgt ttgccgagca ggccggcgtg gtaggccgtg ccacaggcta
     2761 cgatcacgac ccggtctatc tccgagaggt cgatctctac ctcggagagg tcgacgacgc
     2821 cgtcggcatc gatgcggccg aggagcgtcg cgccgagagc agctggctgc tcgtggatct
     2881 cctttagcat gtagtcctcg tagccgccga gctcgacggc ctcggcatcc cagtccacct
     2941 cgaaggcctc acgctcgacg ggctcgcccg aagaggtgaa cacctctacc gaagaggccg
     3001 cgacctcgac gatctcaccg ttctcgacga cgaggaactc gtgcgtctcc ccgagcagcg
     3061 cctgcaccgc gctcgccagg aagttctcac cttcccccag gccgacgacg agcggactct
     3121 gatgtcgggc cgcgacgacc ttacctggct catccacgct gaccgcagcg acggcgaagg
     3181 agccctggag ccgctgaaac accccggcga gcgctatccg caacgtctct ccaacctcga
     3241 ccctctcggc gatcaggtgc gctatcacct ctgtgtccgt ctcggaacga aagatgacgc
     3301 cccgcccctc caactcctcc ttgagctccg agaagttctc tatgatgccg ttgtgtacaa
     3361 tggccacact tccgtctcca ccgagatggg gatgcgcgtt ctcctcgctg gaacgcccgt
     3421 gcgtcgccca gcgggtgtga cccaccccgg tccgcacctg ggagaggtca ccattgcgcc
     3481 cggcaagggc gcgggtgagg ttctcgagtt gcccgatctc tgttatccgc tctatctggc
     3541 cctccttttg aagggccagt cctgccgaat cgtaaccacg gtactcgaga tgcccgaggc
     3601 cctccatgag cacctcgaca caacgctctt taccggcgta acctactatg ccgcacaccg
     3661 acttacctcc ctgtttatcc ggagatggga tcagggaggg cagagaaagg atacagagcc
     3721 tctctcgctc tctgtatccg ggcccgaacg gtgctttgtc cccggcgtca ccg
//
LOCUS       DATMJA010000087         5498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_307895, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000087 DATMJA010000000
VERSION     DATMJA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307895"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VNT12_01975"
     CDS             complement(<1..387)
                     /locus_tag="VNT12_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44910.1"
                     /translation="MRLEPLYRVRFSYAGGWSADLAGPDSAEGRFFFLAEGRISGLFR
                     GANHPHRRSDGTFLPDFQGVIETDDGAEVLFDYRGYGRAYPVGRRQIVLSATHLSEDE
                     RYRWINDSLSVGVGEVRSQEDGPTELV"
     gene            733..921
                     /locus_tag="VNT12_01980"
     CDS             733..921
                     /locus_tag="VNT12_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44911.1"
                     /translation="MRAKPLGLAQSVCEGLVDEVRIVDGALLLTADPAWAWAINIVLV
                     TKGVMVSELRRAGDLRTA"
     gene            complement(1258..2454)
                     /locus_tag="VNT12_01985"
     CDS             complement(1258..2454)
                     /locus_tag="VNT12_01985"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWI44912.1"
                     /translation="MNGANNRTGSGAGHASGGIEPFRIEVPQADLDDLADRLARTRWT
                     NELPPESRGDAVQTGPVPPGWEYGVPLDYVKNLVEYWQTGYDWREWEAKLNSYPQFTT
                     TIDGQDIHFLHVRSPEPDATPLILTHGWPNTVVEYLGLIGALTDPHAHGGDSADAFDV
                     VIPSLPGFAFSGPTTEAGWDAQRTAKAWAELMRRLGYDRYGAHGNDAGAIVAPIQGRL
                     DPEHVIGVHVTQLFSFPSGDPAEFEGLSQEDMEKLRFLQAFNDEMSAYARLQESKPQN
                     LAHALADSPAGQLAWSGQLFGDALSPDEILTNVSIYWLTNTAASAGRFYYENKHAEQP
                     SWPTTAPIGLASFAYDFRPIRRFAERDHTNIVSWSEYDRGSHWAAHDAPDLLIGDIRH
                     FFRRFR"
     gene            complement(2507..3682)
                     /locus_tag="VNT12_01990"
     CDS             complement(2507..3682)
                     /locus_tag="VNT12_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWI44913.1"
                     /translation="MTSDHLDAEIRPFRIDIPQADLDDLADRLARTRWTDEIEGAGWD
                     YGTSLGYLKELTDYWRDGFDWRKQEAELNRSAQFWARIGGFRVHFVHERGKGPHPMPI
                     LLLHGWPDSFYRFHKIIPMLTDPARFGGDPADSFDVVVPSLPGFGFSDRPRERGWGQE
                     RDAALFHTLMTKVLGYERYGAHGGDGGSPISQALARSHPVSVVGIHITDIGYDKAAQL
                     DPSTLSEAEKRYMEALEGWSFEEGGYVMIQGSRPQSLGYGLNDSPAGLAAWIVEKFRA
                     WSDCGGDVERSYSKDELLTNITIYWATQTINPSIRTYYEGMHSWSETPPRVETPVGMA
                     LFAKDPPPDVSLPRETAERFFGDIRSWTEIPRGGHFAAMEEPALLAEDIRAFYRGLR"
     gene            complement(3846..4376)
                     /locus_tag="VNT12_01995"
     CDS             complement(3846..4376)
                     /locus_tag="VNT12_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI44914.1"
                     /translation="MSEGSKPGESGAEIRPAPEGYHTVTPWIISRDAAQLLDFVREAF
                     GAEEIARIHNEDGTIGHAEFRIGDSVVMAFDAKEEWPDTPSFIRLYVEDGDAVYRRAL
                     EAGATSVTEMTDLFFGDRVGRVRDPQGNLWWIQARLEDLDPQEMEKRAAQKEYLDAMQ
                     RVQESLDRELSGRGHG"
     gene            4487..5185
                     /locus_tag="VNT12_02000"
     CDS             4487..5185
                     /locus_tag="VNT12_02000"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI44915.1"
                     /translation="MNAEDSTPLSGRRAEAARNDRKILEAAREVFVADPEAPISKVAE
                     RAGVGISALYRRYASKEELLRRLCSEGLRRYIAEAEAALADEDDPWVAFAAFMCRIVD
                     ADTHSLTLRLAGTFTPTEELYQDSRKAQELNLRLFERTKTADAIRHDVEVDDLSLLFE
                     QVAAVRIGDEERTRQLRRRYLALLLDALRVPLGSPLPGPPPSWEEISRRWENQPSPRS
                     HEARGKSSPLSRRQ"
BASE COUNT          989 a         1749 c         1745 g         1015 t
ORIGIN      
        1 gactagctct gtcggcccgt cctcttgtga gcggacctct ccaaccccca cgctaaggga
       61 gtcattgatc cagcggtaac gttcgtcctc gctaaggtgg gtcgcgctga gtactatctg
      121 cctccgacca accgggtagg ctctaccgta gcctcgatag tcgaagagga cctccgctcc
      181 gtcgtcggtc tctatgacgc cctggaagtc gggtaggaaa gttccatcac tgcgacggtg
      241 tgggtggttg gcccctcgga agagtccgga gatgcgtccc tcggccaaga agaagaaccg
      301 accttcggcg ctatccggac ctgccaggtc cgcgctccag cctccggcgt aggagaaccg
      361 gacacgatac agaggctcca accgcatagc gcatcacccc gcctagacga ccctattgat
      421 ggagctcgca ttgtaccgga ctctattagc agcctacctc ccccttcggc tcctcccgaa
      481 cctggcacct gtgcgatgag tcgcacctcg ccgcgcccta tgcccaaaat tagcgagaac
      541 aacacaaacc cactcccgac acgatacctt tggctacccc gcaaacatta gtcgcaaaaa
      601 ttctcaagaa aaatcgcccg gagacccttg acaacgcgag gcatggaacg ctagtcttac
      661 acagtaaact tacatagtaa gttgcgggta aggaagcgtt gagcgatgac cggaagcgaa
      721 gaaggcttgc tagtgagagc caaaccccta gggctggccc agtcggtctg cgagggattg
      781 gtggacgagg tgcggattgt agacggtgcg ttgctgctca ctgcggaccc cgcatgggca
      841 tgggcgatca acatcgtgct agtcacgaag ggtgtgatgg tcagcgaact gcgtcgtgcc
      901 ggagatctcc gaacagcctg aggactttgc cgcgtacggc actggcggaa ggacaaactc
      961 tatttgcaga ggatagtagc cgcccaggat acgcgtgcgg gcctcacgga tgctaccctg
     1021 cccacgtggc tagagcgcta cgtccagctc cacctcgtcg ccctgtatca aggctcggaa
     1081 ggatataccc agcggcgttg ttccagcttg cgtgagtcca ctagaaggct gtagaacggc
     1141 cttgcccaag ggcctgggaa gacaccttct cagtagtgct aggcggcccc tcctatgacc
     1201 tgtttgtata tctcctccgc gccgaacgcc ccaaggtcga cgccacggtc ctggtatcta
     1261 ccggaacctg cggaagaagt ggcggatgtc gccgatgagg aggtcggggg cgtcgtgggc
     1321 ggcccagtgg ctgccgcggt cgtactcgct ccaggagacg atgttggtgt ggtcgcgttc
     1381 ggcgaacctg cggatcggcc tgaagtcgta ggcgaaactc gccaggccga tgggcgcggt
     1441 ggtcggccag ctcggctgtt cggcatgctt gttctcgtag tagaagcggc ccgctgatgc
     1501 tgcggtgttg gtcagccagt agatcgagac gttggtcagg atctcgtcag ggctgagcgc
     1561 gtcaccgaac agctggccgc tccaggcgag ctgaccggcg ggcgagtcgg cgagagcgtg
     1621 cgccaggttc tgcggcttcg actcctgtag cctggcgtag gccgacattt cgtcgttgaa
     1681 ggcctgcagg aaccgcaatt tctccatgtc ttcctgagac agcccttcaa actccgccgg
     1741 gtcaccggag gggaaggaga agagctgggt aacgtggacg ccgattacgt gctcggggtc
     1801 gaggcggccc tgtatggggg caacgatcgc gccggcgtcg ttgccgtgcg cgccgtagcg
     1861 atcgtagccg agccgacgca tcagctcagc ccaagccttc gcggtgcgct gcgcatccca
     1921 gcccgcttcg gtggtcggcc ccgagaaggc gaagccgggc agggacggaa tgaccacgtc
     1981 gaacgcgtcg gccgagtccc cgccgtgagc gtgcgggtcg gtgagcgcac cgatgaggcc
     2041 aaggtactca accaccgtgt taggccagcc gtgggtgagg atcagcggcg tggcgtcggg
     2101 ctcgggggag cggacgtgca ggaagtggat gtcctggccg tcgatggtag tggtgaactg
     2161 cgggtaggag ttcagcttcg cttcccattc gcgccagtcg tagccggtct gccagtactc
     2221 aaccaagttc ttgacgtagt cgagcggtac gccgtactcc caaccgggcg ggacaggacc
     2281 cgtctggacc gcgtccccgc gcgactcggg cggcagctcg ttggtccagc gggtacgcgc
     2341 cagcctgtcg gccaggtcgt cgagatcggc ctggggcacc tcgatccgga acggctcgat
     2401 cccaccactt gcgtggcctg cgccgcttcc ggtacggtta ttcgcgccgt tcatgacgtt
     2461 cctttctcgc gcaactcttt gggcatccgg agggcgcccg cccgcttcat ctcagcccgc
     2521 ggtagaacgc ccggatgtcc tcggcgagca gtgcgggctc ttccatggcg gcgaagtgcc
     2581 cgccgcgcgg gatctccgtc cagctccgga tgtcgccgaa gaaccgctcc gccgtctccc
     2641 ggggaagaga gacgtctggg gggggatcct tggcgaagag ggccatccca accggcgtct
     2701 ccacgcgcgg cggcgtctcc gaccacgagt gcatcccttc gtagtaggtg cggatggagg
     2761 ggttgatcgt ctgggtcgcc cagtagatgg ttatgttggt gagcagctcg tctttgctgt
     2821 agctccgttc cacgtcgccc ccgcagtcgc tccaggcgcg gaacttctcg actatccagg
     2881 cggccaggcc ggcgggggaa tcgtttagcc cgtaaccgag gctctggggc ctggacccct
     2941 ggatcatgac gtacccgccc tcctcgaacg accagccctc cagggcctcc atataccgtt
     3001 tttcggcttc ggagagggtg gaagggtcga gctgtgccgc cttgtcgtag ccgatgtcgg
     3061 ttatgtggat gccgacgacg gacacggggt gcgagcgcgc cagggcctgg gatatggggc
     3121 ttccgccgtc cccgccgtgc gcgccgtagc gctcgtagcc gaggaccttc gtcattagcg
     3181 tatggaacag cgccgcgtcc cgctcctgac cccagccgcg ctcccgcggg cggtccgaaa
     3241 agccgaaccc cggcagcgaa ggcacgacca cgtcgaagga gtcggccggg tccccgccga
     3301 aacgcgccgg gtcggtgagc atcgggatta tcttgtgaaa gcggtagaac gagtcgggcc
     3361 agccgtgcag gagcaggatc ggcatgggat ggggaccctt gccgcgctcg tggacgaagt
     3421 ggaccctgaa accacctatc cgcgcccaaa actgcgccga ccggttgagc tcggcctcct
     3481 gcttccgcca gtcgaacccg tcccgccagt agtccgttag ctccttgagg tagcccaggc
     3541 tggtgccgta gtcccagccc gccccttcga tctcgtccgt ccagcgggtt cgcgccagcc
     3601 ggtcggccag gtcgtcgagg tcggcttggg ggatgtcgat ccggaacggc cggatctccg
     3661 catccaggtg gtcactagtc atggtcgcaa tctcctctcg gtgtggttga cagcggcgaa
     3721 caggatgtcg tgggttaagg tgccgtgcga cggggcgggc agcgtgtgca gcaggtcggg
     3781 gcccctggaa cggacctcgg atcaagacgt cccaccgtac ggctgaccat cacccaaatg
     3841 caactttatc cgtggcctcg accgctaagc tcgcggtcga gggactcctg gactctttgc
     3901 atggcgtcga ggtactcctt ctgtgctgcg cgtttctcca tctcctgggg atcgaggtcc
     3961 tccaggcgcg cctgaatcca ccagaggttg ccttgagggt ccctcacgcg cccgacccga
     4021 tcaccgaaga acaggtccgt catctcggtc acggaggtgg ccccggcctc caacgctcgc
     4081 cgatatacgg catcgccatc ttcgacgtat aggcggatga agctcggcgt gtccggccat
     4141 tcctctttcg catcgaacgc catcaccacc gagtcgccga tccggaactc ggcgtgcccg
     4201 atggtgccgt cctcgttgtg gattcgggct atctcctccg caccaaacgc ctctctaacg
     4261 aaatctagta gttgggccgc gtccctcgag atgatccacg gcgtgaccgt gtgatagccc
     4321 tcaggcgccg gccgaatctc ggcgccggac tcgcccggtt tgctcccttc cgacacagca
     4381 aactcctcct tcctctacgg attccataaa tagatctatg gaacgaatca ttctgttccg
     4441 tagtataaca gaacggaacg gtttgttcca gggtataatc gctaacatga acgcagaaga
     4501 ctcgacgccg ttgagcggtc ggcgcgccga ggcggcccgc aacgatcgga agatcctcga
     4561 agccgcgcgg gaggtgttcg tcgcagatcc cgaagcaccc atctcgaagg tggccgagcg
     4621 cgccggcgtg ggaatcagtg ctctttaccg ccgctacgcg agcaaggagg agctccttag
     4681 gcggctctgc tccgagggcc tgcgccgcta catcgccgag gccgaggctg cgctggccga
     4741 cgaagacgac ccttgggtgg ccttcgcggc cttcatgtgc cgaatcgtgg acgctgacac
     4801 acactcgctg acgctacgct tggcgggaac cttcacccca acggaggagc tgtaccagga
     4861 tagtcggaag gcgcaggaac tcaacttgcg gctgttcgag cgaaccaaga cggcggacgc
     4921 catccgccac gacgtcgagg tggacgacct ttcgctgctt ttcgagcaag tggccgccgt
     4981 ccgtatcggg gatgaggagc ggacccgcca gcttcgtcgc cggtacttgg cgctgctgtt
     5041 ggacgccctt cgcgtcccgt tgggatcacc gctgcccggc cctccaccca gctgggagga
     5101 gatcagtcgg cgctgggaga accagccttc tccgagatct cacgaggcac gtggaaagag
     5161 ctcaccgtta agcagaagac agtaactcag tagctatctt ggcaagacgg gatcggggta
     5221 tggcggtagg gcatctgctt acggctacgg aacgggagcc accgcgacct ctgtcacaga
     5281 ctcgccgtcc gctccgatcg ccgctcgaaa tgccagacgc ggcgttgtcc caacttgcct
     5341 gggcccacgc ccacgagacg ctcctttggg cgagcaaagg aggcggatct cgccacacct
     5401 tcaactacga cctcataaac agcacagacc ctactgccca gctctcctct gtctggcaca
     5461 tcccttccgt gccaaggagg gaaaaacgtc acggatac
//
LOCUS       DATMJA010000088         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_367485, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000088 DATMJA010000000
VERSION     DATMJA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367485"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VNT12_02005"
     CDS             complement(<1..611)
                     /locus_tag="VNT12_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI44916.1"
                     /translation="MAAQHYDAVVIGAGQAGVPLSQALARSGRRTALIEREHVGGTCI
                     NEGCTPTKTMVASAKTAYVDRRSADYGVHNGHISVDMPEVRGRKRAMVESFRNSGQRR
                     IEATDGLDLIFGEASFTGPRTLAVRTNDGEAPELVADTVFINAGARPANPPIEGLDDV
                     PTLNSTSIMELDELPEHLLVLGGSYVGLEFAQMFRRFGSEVTVV"
     gene            complement(637..>2177)
                     /locus_tag="VNT12_02010"
     CDS             complement(637..>2177)
                     /locus_tag="VNT12_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWI44917.1"
                     /translation="AALESGHKRHQIVRRVEVVRGTMTFRMECSPAFDYARKEHETKI
                     VPGGATFHSPELSLGLASFFPLQQQDDGAFAEFTLQEEQSTVFVLREIEAGEDCGVRL
                     SRMDEEEIFMQTVEYWRRWLSKCTYTGRWREMVRRSALTLKLLTFEPTGAIVAAPTMG
                     LPEGVGGERNWDYRYTWLRDAAFTLYGLLRIGFTEEAEEFITWLGSRCQRSKPDGSLQ
                     LVYGIDGRQNLREETLDHLDGYRGSRPVRLGNGAYDQLQLDIYGELMDAVYLYNKHGS
                     PISYDLWTHLRALINWVCDNWQNEDEGIWETRGGRRNFVYSRLMCWVSIDRGLRLADK
                     RSFPADRDKWLKVRDEIYEEIMEKGWSPEREAFVQSYGDDTLDASSLIMPLVFFLSPS
                     DPRMLKTLDAINRPPKDGGLVSNSLVYRYDVQKSADGLTGEEGTFSLCTFWLVEALTR
                     AGRLEESRLIFEHMLGYANHLGLYAEEIGHHGEALGNFPQAFTHLTLISAAYNLDRAL
                     GSRG"
BASE COUNT          377 a          663 c          692 g          445 t
ORIGIN      
        1 acgaccgtga cctcgctgcc gaagcgcctg aacatctggg cgaactccag gcctacgtag
       61 ctgccgccga gcacgagcag gtgctccggt agctcgtcga gctccatgat tgaggtcgag
      121 ttcagggtcg gcacgtcgtc cagaccttcg atcgggggat tggctggcct ggcgccggcg
      181 ttgataaaga cggtatccgc aacgagctcg ggggcttcgc catcattcgt gcgcaccgcg
      241 agcgttctcg gtcccgtaaa gctggcttcg ccaaatatca gatcaagccc atctgttgct
      301 tcgatgcgcc tctggccgct gttcctgaag ctttccacca tcgcccgctt gcggcctcgc
      361 acctcgggca tgtcgacgga gatatggccg ttgtggacgc catagtcggc cgaacgccga
      421 tctacatagg ccgtcttcgc gctggcgacc atcgtcttgg tgggggtaca gccctcgttg
      481 atgcaggtgc cgccgacgtg ctcgcgttcg atcagggctg ttctgcgtcc cgacctggcg
      541 agcgcctggg agagcggcac cccagcttgg cctgccccga tcaccacggc gtcgtaatgc
      601 tgtgctgcca tagagggaga ctccttccta acggtgctaa cccctgctgc cgagcgccct
      661 atcgaggtta taggccgcgc tgatgagggt gaggtgcgtg aaggcctgcg ggaaattgcc
      721 cagcgcctcg ccatggtgtc cgatctcctc ggcgtagagt ccgaggtggt tggcgtaacc
      781 gagcatatgc tcgaagataa ggcgagattc ctcgagccgg ccagcccgcg tgagcgcctc
      841 gacgagccag aaggtacaga ggctgaaggt tccttcctcg cccgtcaggc cgtccgcgga
      901 cttctgcacg tcgtagcggt acacgaggct gttggagacg aggcctccgt cctttggggg
      961 gcggttgata gcatcgagcg tcttgagcat cctgggatcg ctgggcgaaa ggaagaagac
     1021 gaggggcatg atcaggctcg aggcgtccag ggtgtcgtcg ccgtaggact gcacgaaagc
     1081 ctcacgctcc ggactccagc ccttctccat gatctcctcg tagatctcgt cacggacctt
     1141 cagccacttg tcgcggtcgg cagggaacga gcgcttgtcg gcgagcctca aacctctgtc
     1201 aatggagacc cagcacatga gtctggagta cacgaagttc ctgcggccgc ctctggtctc
     1261 ccagatccct tcgtcctcgt tctgccagtt gtcgcagacc cagttgatca gggccctaag
     1321 gtgggtccac aggtcgtagg agatggggga gccgtgcttg ttgtagaggt agacggcgtc
     1381 catgagctcg ccgtagatat cgagctgcag ctggtcgtag gcgccgtttc cgagcctgac
     1441 gggacgtgag ccgcggtagc cgtcgaggtg gtccagggtc tcctctctca ggttctggcg
     1501 tccgtctatc ccgtacacga gttgtagaga cccgtcgggt ttggatctct ggcagcgcga
     1561 tcccagccag gttatgaact cttctgcctc ttctgtgaac ccgatgcgta gaagcccgta
     1621 gagggtgaat gcggcgtccc tgagccaggt gtaacgatag tcccagttgc gctctccacc
     1681 aacaccttcg ggcaaaccca tggtgggggc tgccactatc gcccctgtcg gctcgaaggt
     1741 gagcaacttc agggtcaggg ccgagcggcg gaccatctcg cgccagcggc ccgtgtaggt
     1801 gcatttggag agccagcggc gccagtactc gacggtctgc atgaagatct cttcttcgtc
     1861 catcctggag agacggaccc cgcagtcttc tccggcttct atctctctga gcacgaaaac
     1921 agtagactgt tcctcttgca gtgtgaactc ggcaaaggcc ccgtcgtcct gttgctgtaa
     1981 ggggaagaac gacgccagtc ccaggctgag ttcaggggag tggaaggtcg ccccgccggg
     2041 gacgatcttg gtctcatgct ctttgcgggc gtaatcgaag gccggggaac actccatccg
     2101 gaaggtcatc gtgccgcgca ccacctcgac gcgacggacg atctggtggc gcttgtggcc
     2161 actctccaga gcggcta
//
LOCUS       DATMJA010000089         2645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_11824, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000089 DATMJA010000000
VERSION     DATMJA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11824"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VNT12_02015"
     CDS             complement(<1..286)
                     /locus_tag="VNT12_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44918.1"
                     /translation="MRFDLPDAYWKKDVALFFGRRFALGLKFLYPLALIVPVAAGGIP
                     GQYAAAVIGIVAIMAGTFGAGETLTSDLNDGILARVALTPLSPRKLVFQIL"
     gene            complement(276..1238)
                     /locus_tag="VNT12_02020"
     CDS             complement(276..1238)
                     /locus_tag="VNT12_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI44919.1"
                     /translation="MRSNTQNAPPALEAKNISRLYKSSGRGVSDVSMRVERGEVFGLM
                     GPNGSGKSTLLRVLSTAIAPNSGGFRVGGVDGLREKREVRARMGLMVDRPTHYDDLTG
                     RANAYFFARAYGMEEAKAEDALAELFDYFDLAEYADDRVKTYSYGMGKKLALIEALAH
                     GPEVLLLDEPSLGLDYTSQLAYQSRIRDLAGSGAAVLLSSNQVDEVEALCDRAAFLHR
                     GKVVAEGTPEDLISRVEGVRRIVATLRNPVEYGSIAEVGGVERIVPEGRALILHCEER
                     PGIVADVVAGIVRSGGDIVNLSVQRPNLEDVFVELTGEVLRDEV"
     gene            1381..1569
                     /locus_tag="VNT12_02025"
     CDS             1381..1569
                     /locus_tag="VNT12_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HWI44920.1"
                     /translation="MAETREFTWDPEADRRIRRSPFLMRRFIRKRIEDRVASRGRTHV
                     TEADVDESASMYRQYRFK"
     gene            complement(1818..2570)
                     /locus_tag="VNT12_02030"
     CDS             complement(1818..2570)
                     /locus_tag="VNT12_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWI44921.1"
                     /translation="MNEHPALLLEDVYKSFGENEVLKGIDLTVEPHEVVCLIGASGSG
                     KSTLLKCVNLVEPIDSGRVVVEGEEITAAGVDTNRIRRRIGIVYQSFNLFPHMTVLRN
                     ITLDPREALGTNRAEAEKNARSLLERFGLLDKQNEYPDRLSGGQQQRVAIVRALAMRP
                     HIMLLDEVTSALDPELVVDVLAVIKELAREGMTMLIATHEMGFARDIADRVCFLEAGR
                     ILEQGKPERIFTAPEHPRTRQFLGRIIEAGRL"
BASE COUNT          495 a          837 c          813 g          500 t
ORIGIN      
        1 ccagtatctg gaagaccagt tttcgcggcg ataggggggt tagggctacg cgcgccagga
       61 tgccgtcgtt caggtcgctt gtgagggtct ctccggcacc gaacgtgccg gccattatcg
      121 ccacgatacc tatgacagcc gcggcgtact gccccggaat tccgcccgct gcaacgggga
      181 cgatcagagc gagcgggtat aggaacttca ggccaagagc gaacctcctc ccgaagaaca
      241 gcgcgacgtc cttcttccaa taggcgtccg ggagatcaaa cctcatccct gagcacctcc
      301 ccggtcaact ctacgaacac gtcctccagg ttgggcctct gcacagacag gttcactatg
      361 tccccaccag agcggacgat gccggccacg acgtcggcga ctatgccagg acgttcctcg
      421 cagtgtaaga tgagagccct tccctccgga acgatgcgct ctacaccgcc gacctcagct
      481 atagagccgt actccacggg attgcgtaac gtcgccacaa tgcgcctgac accctccaca
      541 cgggagatga gatcctccgg cgttccctcg gcgacgacct tcccccgatg cagaaacgct
      601 gctctgtcgc acagagcctc gacctcgtcg acctggttgc tcgagagcag gaccgcggca
      661 ccggagccgg cgaggtccct gatcctgctc tggtaggcga gctgcgaagt atagtcgagc
      721 ccgagcgagg gctcgtccag cagcagcacc tccggcccat gcgccagcgc ctcgatcagc
      781 gcgagtttct tgcccatacc gtaagaatac gtcttgaccc tgtcatccgc atactcggcc
      841 aggtcgaaat agtcgaagag ctctgcgagt gcgtcctcgg ccttggcttc ctccatcccg
      901 taggcgcggg cgaagaagta cgcgttcgcc ctgcccgtga ggtcgtcgta gtgagtcgga
      961 cggtcgacca taagacccat gcgggcgcga acctcccgct tctctctcag accgtccacc
     1021 ccgcccacac ggaaaccgcc ggagttggga gcgatggctg tggagagtac gcgcaggagc
     1081 gtgctcttac ccgagccgtt cggtcccatt agaccgaaga cctcgccgcg ctccacgcgc
     1141 atacttacat cagagacgcc gcgcccgctg ctcttgtaga ggcggcttat gttcttcgcc
     1201 tccagggccg gcggggcgtt ctgtgtgttg gatctcatgg cacgagaatt gtagcgccct
     1261 ccccgctgga cggttgtcag acgcatcacc ccgttcttca aggccgcaag gccagaccgc
     1321 gactgacatg gccggaagcg cgggaatata atccctctgt aaactcagag gagaatatgc
     1381 atggctgaga ctagagagtt cacctgggac cccgaggccg accgcaggat aaggcgcagc
     1441 ccgttcctga tgcgccgctt tattcgcaag cgcatcgagg acagggtggc atcgaggggc
     1501 cgtacccacg tcaccgaggc cgacgtagac gagagcgcca gcatgtaccg ccagtaccga
     1561 ttcaagtaag ggcagttcgc gaaccgcccc aaaaatatcc gggccggaga gccgctcttt
     1621 tggcgaacga ctaccgaccc ggtccgcgga cctgtaaggg cccgcggatg gttgttggct
     1681 agctagtcca ctggaccctt tcctcctagt cgaggtgcaa caaccagggt catgttacca
     1741 tcactcacga gccccgcgct acaactttcg gaccatttcg tgcgcctgta cgaacgtgcc
     1801 gccacatacg gtggggccta caaccgaccg gcctcgatta tgcgtccgag gaactggcgg
     1861 gtgcgggggt gttccggggc ggtgaagatc ctctctggct taccctgttc gaggattcga
     1921 ccagcttcga gaaagcagac gcggtctgcg atgtcgcggg cgaagcccat ctcgtgggtc
     1981 gctatgagca tggtcatccc ttcacgggcg agttccttga tgacggcgag gacatcgacg
     2041 accagttcgg ggtcgagggc gctggtgacc tcgtcgagga gcatgatatg ggggcgcatc
     2101 gcgagcgccc tcactatggc gacccgctgt tgctggccgc cggagagtct gtcggggtac
     2161 tcgttctgct tgtccaggag cccgaaacgt tccaggagtg agcgggcatt cttctcggcc
     2221 tcggcgcggt tcgtccccaa ggcctcgcgc gggtcgagag tgatgttgcg caggaccgtc
     2281 atgtgtggaa agaggttgaa ggactggtag acgatgccta tgcgacgccg gatgcggttc
     2341 gtgtcgacgc ctgcggcggt gatctcttct ccctccacaa cgacccggcc agaatctatc
     2401 ggctcgacga ggttgacgca cttgagcagc gtggacttgc ccgagcctga ggccccgatc
     2461 aggcatacca cctcgtgtgg ctcgaccgtc aggtctatcc ctttcagaac ctcgttctct
     2521 ccgaaggact tgtagacgtc ttccaggagg agggcgggat gttcgttcat gccagggctc
     2581 ctgcctctcg gcggcgtttg tcgcgggcga tcagacggtc cgtaaatcgg gcgaggggga
     2641 tggtg
//
LOCUS       DATMJA010000090         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_248672, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000090 DATMJA010000000
VERSION     DATMJA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248672"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VNT12_02035"
     CDS             <1..1095
                     /locus_tag="VNT12_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWI44922.1"
                     /translation="GFLAENAAFAAACREAGLTFVGPSAKAIEKLGNKSAARRLASEA
                     KVPVVPGSDDASSADEAAQTAGGIGYPIMVKAAAGGGGRGIRVAEDEDELRKAVQVAR
                     REAEAAFGDGTLYLEKLLVAPRHVEVQVMGDHEGNVIHLYERECSMQRRRQKVLEEAP
                     SPGISLGVREKMAEAAVRLAREAGYANAGTVEFLVEDEEFYFIEMNTRIQVEHPVTEM
                     LTGVDLVKEQIRIAAGDPLSLAQKDVPMVGHAMEFRINAEDPDQDFMPSPGEISRLEV
                     PGGPGVRVDSAIYQGYQIPPFYDSLVGKLIVWALTREEAISRARRALREYRLEGIKTT
                     IPLHLRLLKGEAFRSGEYHTGYLEELLNGD"
     gene            complement(1226..>2019)
                     /locus_tag="VNT12_02040"
     CDS             complement(1226..>2019)
                     /locus_tag="VNT12_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI44923.1"
                     /translation="PYAYRCPAGRDCSGGCVGDCFASIEKAFVSTVHPSSVAAIIVEP
                     VSGEGGFIPFPDFYMRRLRELCDEHGIVMIVDEVQTGFGRTGKMFAVEHSGVEPDIIT
                     TAKSMGGGLPIAGVTGKAEIMDSVHVGGLGTTYGGNPIACAAALAVLEAFEEEDLLAR
                     ANIVGERVMAAMREIQDRHPDFVGDVRGRGPMAAMEFVKDAEGRTPDKERTAKVIEGA
                     LQEGLLLLSAGQYGNCIRTLMPFVITDEELEEGLAVLGRAVDSAA"
BASE COUNT          379 a          626 c          661 g          353 t
ORIGIN      
        1 gggtttctgg ccgagaacgc cgcgtttgca gcggcgtgcc gggaggccgg gctcactttc
       61 gtcgggcctt cggccaaggc aatcgagaag ctggggaaca agtccgccgc gcgtcggcta
      121 gctagtgagg ccaaggtgcc ggtcgtgccc ggttcggacg atgcctcttc cgctgacgag
      181 gcggcacaga cagctggtgg gataggatac ccgatcatgg tcaaggcagc ggcgggtggc
      241 ggtggccgcg gcatcagggt ggccgaggat gaggacgagc tcagaaaggc cgtacaggtg
      301 gccaggcgcg aggccgaggc cgctttcggg gacgggacgc tctacctgga gaaactgctc
      361 gtcgcgccga ggcacgtcga agtgcaggtc atgggcgacc acgaggggaa cgtgatccac
      421 ctctacgagc gcgagtgctc gatgcagcgc cggcgacaga aggtgctcga ggaggcccct
      481 tcgccaggta taagcctagg ggttcgcgaa aagatggctg aggcggcggt tcggttggca
      541 agggaggctg ggtacgcgaa cgctggtacc gtggagtttt tggtcgagga cgaagagttc
      601 tactttatag agatgaacac gcgtatccaa gtcgagcacc ccgtgaccga gatgctcacc
      661 ggcgtggacc tggtgaagga gcagatccgg atagctgctg gagatccctt gagtctcgca
      721 cagaaagacg taccgatggt cgggcacgcg atggagtttc ggattaacgc cgaggacccc
      781 gaccaggact ttatgccgtc tccaggcgag atctcccggc tcgaagtccc aggcggccca
      841 ggcgtcaggg tagactcggc catctaccaa gggtaccaga tccccccgtt ctacgactcg
      901 ctggtgggca agctcatagt ctgggccctg accagagagg aagccatcag cagggccaga
      961 cgcgctctcc gcgaataccg cctcgagggc ataaagacga cgatcccgct ccacctcagg
     1021 ctcctgaaag gagaagcctt ccgatccggc gagtaccaca caggctacct ggaagagctg
     1081 ttgaacggag actgaacgcc tcaagtcgct cgaggtctga gttctttaca acgaaagctt
     1141 cgctcaccga ctcatcatcc cctcaacgaa atacgggccg cacatgcgac ccgtacaatt
     1201 ctagctgagt ggactagctg cggtgttacg cggctgagtc tacggcgcgt ccgagaacgg
     1261 ccagtccctc ctccagttcc tcatcagtga tgacgaaggg catgagggtg cggatgcagt
     1321 tgccgtactg gccagcgctg aggagcagga gcccttcctg gagggcgcct tcgatcacct
     1381 tcgcggtccg ctccttgtcg ggggtgcggc cctctgcgtc cttgacgaac tccatcgcgg
     1441 ccatcggtcc cctgccacgc acgtcgccga cgaagtccgg gtgccggtcc tggatctcgc
     1501 gcattgcagc catgacgcgc tcgccgacga tgttggcacg cgcgaggagg tcttcctcct
     1561 cgaaggcttc tagcacggcg agggcggcgg cgcaggctat cgggttgccg ccgtaggtgg
     1621 tgccaaggcc cccgacgtgg acggagtcca tgatctccgc tttccccgta acgccggcta
     1681 tcgggagtcc accgcccatg ctcttggccg tcgtgatgat gtccggctcg acgcctgagt
     1741 gctcgacggc gaacatcttg cccgtcctgc cgaaacccgt ctgaacctcg tctactatca
     1801 tcacgatgcc gtgctcgtcg cacagctccc gcagtctcct catgtagaag tcggggaacg
     1861 gtatgaagcc gccctcccca ctcaccggct cgacgatgat cgccgcgacg ctgctcgggt
     1921 gcaccgtgct cacgaacgcc ttctcgatgg atgcgaagca gtcgcccacg cagccgccgg
     1981 aacaatccct gcccgctggg cagcggtacg cgtagggcg
//
LOCUS       DATMJA010000091         3730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_130709, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000091 DATMJA010000000
VERSION     DATMJA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130709"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VNT12_02045"
     CDS             complement(<1..567)
                     /locus_tag="VNT12_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HWI44924.1"
                     /translation="MLRVLLLAAAAAVFGLLYLLSAEFRSETNRALGILGRGDIAGLR
                     DYIVSFGLWAPVASCFLMVLQALVAPVPSFLITFANGLAFGVFWGWMLSLFGHVLAAA
                     VCFGISRALGRVPVEVLVGKTGLESADRWFARWGVYAVFVGRLLPGVAFDAISFSAGL
                     TRMRFRSFMAATALGIFPQTFLYSYLGRQ"
     gene            complement(714..1625)
                     /locus_tag="VNT12_02050"
     CDS             complement(714..1625)
                     /locus_tag="VNT12_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWI44925.1"
                     /translation="MKAVRSLLAVPAARRKMVEKALTSEADAVFLDLEDAVAPDSKAA
                     ARGDVVSALKELDWRGRQGLYRANALDTPYFYRDLIEVVEEAGNSLDAIMVPKVNRPE
                     DLHAVSMLLSQLELAMELEKGKIRLEAQIESAEGLTNVDSIASATSRLEALHFGPGDF
                     AASVSMPQTSIGVMDEWDEVYSGHRFHYAMQRIVVAARAAGLRVLDGPVADYGDEGGL
                     RRSCLIARSLGFDGKWCIHPAQIAVVNETFSPTDREVEWARKVVAAYEEAKATGSGSV
                     SVDGQMVDAASIKLARNTLGKVPEKDS"
     gene            complement(1627..3156)
                     /locus_tag="VNT12_02055"
     CDS             complement(1627..3156)
                     /locus_tag="VNT12_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWI44926.1"
                     /translation="MTERNGQGGAGGAGDDRYDATDATQRRSDEGTAREDAMVGAIGA
                     GSPEDVGQSTFAATTDSLQHTMGEDRPESGSYAFPAFDDHHPPDKALIDECVHCGFCL
                     PTCPTYVLFGEEMDSPRGRIYLMNKGLDEEPMNDEMVRHWDLCLGCMACVTACPSGVQ
                     YDKLIESTRQQVERRYERRADDRAFREMIFQFFPYPNRLRAVAAPMRLYQRFGIGDKL
                     RRAGIMQRIPARMRAMEALLPDLPREEKIPDLTGPVGEKRRRVGVLTGCVQRVFFSPV
                     NAATVRVLAAEGCEVVAPKQGCCGALSTHAGREEESLDFARKTIDTFEDLNLDNVVVN
                     AAGCGSTMTEYGYLLRDDPEYAERAKAFSAKVRDVSEFLQELGPVAERNPLPVTAAYH
                     DACHLAHAQGVRKQPRQTLRQIPGIEIKEIREAEICCGSAGIYNMVEPEPAAELGERK
                     ANNILKTGAQIIVTSNPGCMLQIQSSLKKMGHGGLPMAHPVEVLDASLRAEPVEMLLD
                     K"
     gene            complement(3156..>3730)
                     /locus_tag="VNT12_02060"
     CDS             complement(3156..>3730)
                     /locus_tag="VNT12_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWI44927.1"
                     /translation="HSQVEATAVELHYGENEKQLAVLLESIPGGIDAKAETASFLLKQ
                     FGEVRTLSDEEAEHPGPLTPPEVADEVVLKIGAPPVDLAAVLESVLGAAERKGLAHPR
                     ITGHAGTGVTLVGFSEENEDGAAGFVEEMREIWVRRGGNVTLQRAPLTLKQRVGTWDN
                     GGDYLGLVRRVKEKFDPRGGMNPGRFLGGN"
BASE COUNT          666 a         1250 c         1148 g          666 t
ORIGIN      
        1 ctggcgtccc aggtacgagt agaggaacgt ctgggggaag atgccaaggg ccgtggccgc
       61 cataaagctc cggaaccgca tcctcgtgag ccccgcggag aaggagatgg cgtcgaaagc
      121 cacgccaggc aggagccgtc ctacgaacac ggcatagacc ccccatcgcg cgaaccagcg
      181 gtcggccgat tcgagccccg tcttaccgac gagcacctcc acggggaccc tgccgagggc
      241 gcgtgagatc ccaaagcaca cggccgccgc caacacgtgc ccgaacaggg agagcatcca
      301 gccccagaac accccgaaag cgagcccgtt cgcgaaggtg atgaggaagg acggtacggg
      361 cgcaaccaac gcctgcaaca ccataagaaa acacgaggcc acaggcgccc acagaccgaa
      421 cgagacgatg tagtctctga gcccggcgat gtctccacgt ccaaggatcc ccaacgcccg
      481 attcgtctcg gaccggaact ccgccgaaag gagatacagc agaccgaaga ccgccgccgc
      541 cgccgcgagc aacaagaccc gcaacaagcg taccgtacga gcgctcagtc gtgcgggtgg
      601 cgccaagaga ggccgttgct cgggcccgtt catccgttca agttataccg ccgccaggcc
      661 agaagccctg cggtgcacgg agcctgtgga acggtgacct cgtcgatctc ggctcaagag
      721 tccttctccg ggaccttgcc aagcgtattg cgcgccagct ttatggaggc ggcgtcaacc
      781 atctgcccgt ctacgcttac cgagcccgag cctgtggcct tcgcctcctc gtaggctgcg
      841 acgaccttcc tcgcccactc cacttcccga tcggtaggtg agaaggtctc gttgaccacc
      901 gctatctggg ccggatggat gcaccacttg ccgtcgaagc ctagtgagcg ggcgatcagg
      961 cagctcctgc gcagcccccc ctcgtcgccg tagtcggcga cggggccgtc gaggacgcgc
     1021 aggcccgcgg cgcgggccgc gacgacgatg cgctgcatgg cgtagtggaa gcggtggcca
     1081 gaatacactt cgtcccactc gtccatcaca ccaatactcg tctggggcat gctcacgctg
     1141 gcagcgaaat ctccagggcc gaagtggaga gcttcgaggc ggctcgtggc gctggctatc
     1201 gagtctacgt tggtcaggcc ttcggcgctc tctatctggg cttcgagcct tatcttgccc
     1261 ttttccagct ccatcgccag ctccagctgg gagaggagca tggagacagc gtggaggtct
     1321 tcggggcggt tcactttggg gaccatgatg gcgtctaagg aattaccggc ctcttcgacc
     1381 acctcgatca ggtccctgta gaagtaaggc gtgtccagtg cgttggcccg gtagagtccc
     1441 tgacgaccac gccagtcgag ctccttcaga gcagagacaa cgtccccgcg ggccgcggcc
     1501 ttgctgtcag gggctacggc atcttcgaga tcgaggaaca cggcgtcagc ctccgaagtg
     1561 agcgccttct cgaccatctt ccgtcgcgct gcgggaacgg cgaggaggga acggacggct
     1621 ttcatgttac ttgtcgagta gcatctctac cggctctgcg cgcagagagg cgtccagaac
     1681 ctccacaggg tgggccatcg ggaggccgcc gtggcccatc ttcttcaggg aagattgtat
     1741 ctggagcatg cagcctgggt tggaggtcac gataatctgc gcccctgttt tcaggatgtt
     1801 gttcgccttg cgctcaccga gctcggcggc aggctcaggc tcgaccatgt tatagatgcc
     1861 cgcagatcca cagcagatct cagcctcacg gatctccttt atttctatcc caggtatctg
     1921 cctgagggtc tggcgcggct gcttacggac tccctgcgca tgcgccaggt ggcaggcgtc
     1981 gtggtaggcc gccgtaacgg gcaacggatt tcgctcagcc acagggccaa gttcctgaag
     2041 gaactccgag acatcgcgga ccttcgcgct gaaagccttc gccctctccg cgtactcagg
     2101 gtcgtcgcgc agcaggtaac cgtactccgt catcgtcgag ccacagcccg cggcgttgac
     2161 gacgacgttg tccaggttca ggtcctcgaa ggtgtctatg gtcttgcggg cgaagtccag
     2221 ggactcctcc tcgcggcccg cgtgggtgga gagggcgccg caacaacctt gcttgggggc
     2281 caccacctcg caaccctcgg cggccagtac gcggaccgtg gcggcattga ccggggagaa
     2341 gaagacccgc tgcacgcagc ccgtcagcac accgaccctg cgacgcttct cgcctacagg
     2401 gcctgttagg tcagggatct tctcttctct tggtaggtcc ggcaacagcg cttccatagc
     2461 ccgcatcctg gccgggatgc gttgcatgat gccggccctg cgcagcttgt ctccgatccc
     2521 gaagcgctgg tagaggcgca taggggctgc gacggcccga aggcggttgg gataggggaa
     2581 gaactggaag atcatctccc tgaaagccct gtcgtccgcc cggcgctcgt agcggcgctc
     2641 gacctgctgg cgcgtagact cgatgagctt gtcgtactgc acgccggatg ggcaggccgt
     2701 aacgcaggcc atgcacccga ggcacagatc ccagtgacgc accatctcgt cgttcatcgg
     2761 ctcctcgtcg aggcccttgt tcatcaggta gatgcgcccg cgaggagagt ccatctcttc
     2821 cccgaatagc acgtaggtag ggcacgtcgg caggcagaag ccacagtgca cgcactcgtc
     2881 tatgagagcc ttgtccggcg gatggtggtc gtcgaaggcc gggaatgcgt agctgccgct
     2941 ctcgggcctg tcctcgccca tggtgtgctg cagagagtca gtggttgccg cgaaggtgct
     3001 ttgccccacg tcctccgggc tcccggcgcc gatggcccca accatagcgt cctcgcgcgc
     3061 cgtcccctcg tcgctccggc gctgcgtggc atccgtcgcg tcgtagcggt cgtcacctgc
     3121 tccaccggct ccgccttgac cgtttcgctc cgtcactagt tacctcccag aaacctgccg
     3181 gggttcatgc cgccccgggg atcgaacttc tccttcaccc tccggacgag accgagatag
     3241 tctcccccgt tgtcccaagt gccaaccctc tgcttcaagg ttaatggagc gcgttgcagg
     3301 gtcacgtttc cgccgcggcg gacccagatc tcacgcatct cctccacgaa cccggcggca
     3361 ccatcttcgt tctcctcgga gaacccgacg agggtaacgc cggtccccgc gtgacccgtg
     3421 atgcgcggat gcgcgagtcc cttgcgctcc gccgcgccca ggaccgattc gagcactgcg
     3481 gccagatcca ccggcggagc cccaatcttc aggacaacct cgtcggcgac ctcgggcggg
     3541 gtgaggggac cgggatgctc cgcctcctcg tccgagagcg tccgcacctc cccgaactgc
     3601 ttgaggagga aggaggcggt ctccgccttg gcgtctatgc caccggggat gctctccaga
     3661 agcactgcca gctgtttctc attctcgccg tagtgcaact cgactgccgt agcctctacc
     3721 tgagagtgaa
//
LOCUS       DATMJA010000092         2837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_367869, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000092 DATMJA010000000
VERSION     DATMJA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367869"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VNT12_02065"
     CDS             complement(<1..1112)
                     /locus_tag="VNT12_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWI44928.1"
                     /translation="MLGKVLVANRGEIALRVIRACQELEISVVAVYSDADSEALHVRH
                     ADEAINIGPSPAGKSYLNVEALLEAAKETGAEGIHPGYGFLAENASFAAACRDAGLTF
                     IGPSAEAIEKMGNKSAARKLAQWADVPVVPGSEESASADDAVETAGGIGYPVMVKAAA
                     GGGGRGIRVAGDEEELRKAVQVAKREAESAFGDGTLYLEKLLVGPRHVEVQVMGDHDG
                     NVIHLYERECSMQRRRQKVLEEAPSPGISPDVREKMTEAAVRLARETEYANAGTVEFL
                     VDGDDFYFIEMNTRIQVEHPVTEMLTGVDLVKEQIRIAGGEPLSVEQDDVSMIGHAME
                     FRINAEDPEKDFMPSPGEITFLDVPGGPGVRVDSAI"
     gene            complement(1186..1740)
                     /locus_tag="VNT12_02070"
     CDS             complement(1186..1740)
                     /locus_tag="VNT12_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HWI44929.1"
                     /translation="MHLNVAMMTRTALMAAVTAVAAQIAIPLPFSPVPFTLQVLAVIL
                     SGLLLGSRYGALAQAIYVLVGAVGVPVFAQFSGGLGVVLGPTGGYLLSYPLAAAIAGL
                     AAHTAANATRRKALWASFLWGCTGLAVIYALGATWLAVITRLPFAAALAQGVLPFVPF
                     DLIKVALAALVASAAAPAIGSSRT"
     gene            1876..2781
                     /locus_tag="VNT12_02075"
     CDS             1876..2781
                     /locus_tag="VNT12_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HWI44930.1"
                     /translation="MGRSIETELPQARYSYGGDEYVFVELAEAMSLKVNFRAMAITNR
                     LREENIPGVTDICPSNASYMIRIDPDELHPDDLISRLKEIDEEVGDIRGFELNTRVVD
                     VPVMMEDPWTHETLMRFRDRHQDPDATDLEYSARINGYDSKEDFIEALLGSPWLVTMI
                     GFVPGLPWCYQLVPPEEQIEVPKYVRPRTYTPERTFGFGGGFAVVYSVEGAGGYQLFG
                     RSPVPVLDVQQRLKDFQDSIVFPKPGDVWNYRRIDREEFDRLEVEVEEGTFEYRKKDF
                     VFHPDRALENPHAYNEEILGVLYDD"
BASE COUNT          519 a          909 c          883 g          526 t
ORIGIN      
        1 tagatggccg agtccacacg gacgccgggg ccgccgggga cgtcgagaaa agtgatctca
       61 cccggcgaag gcatgaagtc cttctcgggg tcctcggcgt tgatccgaaa ctccatcgcg
      121 tggccgatca tcgacacgtc gtcttgctca acgcttaaag gctcaccccc ggctatcctg
      181 atctgctcct tcactaggtc cacgcccgtc agcatctctg tgaccgggtg ctctacctgt
      241 atgcgtgtgt tcatctcgat aaagtagaag tcgtctccgt cgaccagaaa ctccacggtc
      301 ccagcgtttg cgtactccgt ctcccgggcc aggcggacgg ctgcctcggt catcttctct
      361 cgaacgtcgg ggcttatgcc tggtgacgga gcctcctcca gcaccttctg cctccgacgc
      421 tgcatggagc actcccgctc gtagaggtgg ataacgttgc cgtcgtggtc acccatcacc
      481 tggacctcca cgtggcgcgg cccgacgagc agcttctcca ggtagagcgt cccgtcaccg
      541 aaggcggact cggcctcgcg cttcgcgacc tgtacagcct tgcggagctc ctcctcgtcc
      601 ccggccaccc ggatgcccct gccgccgcct ccagccgctg ccttgaccat gacggggtag
      661 ccgattcctc cggccgtctc gacggcatcg tcggccgagg cggactcttc cgaaccgggc
      721 acaaccggca cgtcggccca ttgcgccagc ttgcgggccg cagacttgtt acccatcttc
      781 tcgatggctt ccgccgaagg cccgatgaag gtgagcccgg cgtcccggca agccgcggcg
      841 aacgaggcat tctcggcgag gaaaccgtag cctggatgga tcccttcggc cccggtttct
      901 ttggcggctt ctagcagggc ctccacgttc aggtagctct tgccggctgg cgaaggaccg
      961 atgttgatgg cctcgtcggc gtggcggacg tggagagcct cggagtcggc gtcggagtag
     1021 acggcgacca cggatatctc cagctcctgg cacgccctta tgacgcgcag cgcgatctca
     1081 ccgcggttgg caaccagtac cttccccaac atgccgcgct cctccctctg tgcctcacgt
     1141 aatccggcgt catggtaccc gcgcttgcca cctcgcgctc ccccatcatg tccgcgagga
     1201 tcctatggcc ggggcggccg cgctcgccac gagtgcggcg agcgccacct ttatgaggtc
     1261 gaacggcacg aacggcagga cgccctgcgc cagggcagca gcgaagggaa gtcgtgtaat
     1321 cacggcgagc caggtcgccc cgagagcgta gattacggca agacctgtgc atccccagag
     1381 gaagctagcc cacaatgcct tgcggcgggt ggcattggcc gcggtgtggg cggcgaggcc
     1441 agctattgcg gcggctaggg ggtacgagag gaggtagcct cccgtcgggc cgaggacgac
     1501 gccgagcccg ccggagaact gggcgaagac gggaacaccg accgccccaa ccagcacgta
     1561 gatcgcctgc gccagcgccc cgtatcttga cccgagtaag agtccggaga ggatgaccgc
     1621 gagaacttgc agggtgaagg ggactgggga gaagggcagc ggtatggcaa tctgtgccgc
     1681 taccgctgtc accgcggcca ttagcgccgt ccgggtcatc atcgcgacgt tcaagtgcat
     1741 cttactcttt cgcgcagagg tctggctggc cacatctgtt gttgattaat ctgcctttta
     1801 agaatacatt gaccggaatt tccggcgcta gtagtatgcc ggcactgttc agggaagtgg
     1861 ggtgataagg gcatcatggg ccgcagcatc gagaccgagt tgcctcaggc acggtacagc
     1921 tacggtgggg acgagtacgt cttcgtcgag ctcgctgagg cgatgagctt gaaggtgaac
     1981 ttccgggcga tggcgatcac gaataggctt cgggaggaga acatccctgg cgtgacggac
     2041 atctgccctt ccaacgcttc gtacatgatc cgtatagatc cagacgagtt gcatccggac
     2101 gacctgatct cacgcctcaa agagatagac gaggaggtcg gcgacatacg cggcttcgag
     2161 ctgaatacgc gggtcgttga cgtgcccgtc atgatggaag acccctggac ccacgagact
     2221 ctgatgcgct tccgcgaccg tcaccaggac ccggacgcta ccgacctgga atattcagcc
     2281 cgtataaacg gctacgactc gaaggaggac ttcatcgagg cgcttctggg ctctccgtgg
     2341 ctggtgacca tgatcgggtt cgtgcccggg ctcccctggt gctaccagct cgtccctccg
     2401 gaggagcaga tcgaggttcc caagtacgtg agaccccgca cctacacccc cgagcggacc
     2461 ttcggcttcg gcggcggctt cgcggtggtt tactccgtcg aaggggcagg cggctaccag
     2521 ctctttggtc gatcccctgt cccagtgctc gacgtgcagc agcgactgaa ggatttccag
     2581 gactccattg tcttccccaa gccaggcgat gtctggaact accgtcgcat agaccgcgag
     2641 gagttcgacc ggctcgaggt cgaggtcgag gagggaacct tcgagtacag gaagaaagac
     2701 ttcgtcttcc acccggatcg cgcgctcgag aacccacacg cctacaacga ggagatactg
     2761 ggggtgctct atgacgatta gggtgagaca acccggcctc ctgaccaccg tgcaggacac
     2821 ggggcgcttc ggtgagt
//
LOCUS       DATMJA010000093         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_841851, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000093 DATMJA010000000
VERSION     DATMJA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841851"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VNT12_02080"
     CDS             <1..768
                     /locus_tag="VNT12_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI44931.1"
                     /translation="ELYGDPEGAIQAMQDAVDAGAPVPENKAWAYVQLGNLRFTTGQL
                     EEAAKDYDLATKTLPNYPPALAGQARIAAARGDLEDAAALYQEAFDRMPLAEYAILLG
                     DVYAEMGEKQKAAEQYDLVRVIDKLYRANGVNTDLEIALFYADHDMKLQTSLEKARSA
                     YDARPSIHAADALAWSLYKTGNYKEAKKYSSEALKLGTRDPLKLFHAGMIAKALGRRE
                     QAREYLQQAVDLNPHFSLLYSDEAVDSLKALNAKGED"
     gene            768..2417
                     /locus_tag="VNT12_02085"
     CDS             768..2417
                     /locus_tag="VNT12_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI44932.1"
                     /translation="MFRWSWLLACLAFAVFASLIPREAQAHPLGNFTINHYSRLEFAD
                     EKARIDYVLDFAEIPTFQQKGKLDPNEDGKLSDAEARAYLDAKLPSLVENLHLRVGKE
                     DLLLRVLDSSAEYRPGQGGLPILRIEARLLADLPKDWEDHGVGHYADRTYENRLGWRE
                     IVVKGGPGVAIKGSTAPSSGVSDELRKYPNDMLSSPLDVRQATFTLVPGEGSFGDGPV
                     GEAARNYSTSDYSFKSNGYISKLYAWLNSLISFTSPSPTVILISLLAALLWGALHAFT
                     PGHGKTIVAAYLIGDRGTARHAAFLGLTVTLTHTLGVFALGGVAIYLSRYILPEALFP
                     WLSVVSGLLVVVIGLSLLFSRSRGLFGTGSGKARHTKLRHADGPHTHSHDGHDHSYAY
                     ARVAHSHSHGDHEHSHDHVHSAHSHSHGDHEHTHSHGGHTHTHLPPGADGSKAGIRSL
                     VALGVSGGLVPCPAALVLLLSAISLGRLGFGMVLVVVFSMGLAVVLTGIGLLMVYARR
                     SFERYSFEARIPLFLPVASAAVISFVGLVIVLGAISQTGLL"
     gene            2507..2632
                     /locus_tag="VNT12_02090"
     CDS             2507..2632
                     /locus_tag="VNT12_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44933.1"
                     /translation="MKPELVDELGVDFYDRFYHPEVLSETQRRHANRWHDLEAQT"
     gene            2629..3039
                     /locus_tag="VNT12_02095"
     CDS             2629..3039
                     /locus_tag="VNT12_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44934.1"
                     /translation="MNRSNVAWVLRADGLFNLFAGLVLLTFYRPVVALIGWPNTQMPI
                     YANVLGAALIGLSLTVILVSRHPEQSRGIILSGVLTKALAGVTILYWVFIAGIDLPSP
                     WLLPAAVGVQVLFALGEAAYCLPSRTGGPNRRVA"
     gene            3039..3602
                     /locus_tag="VNT12_02100"
     CDS             3039..3602
                     /locus_tag="VNT12_02100"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWI44935.1"
                     /translation="MKAGTGAGIACAIGVAGGIVIGARAVKIYDWRTEWTIPAPMPIV
                     YEAMTSREAVREWWPDMELVDDSGDDDLHVGSTVSFRVHQAPDVARLAPPFRIHCLYT
                     DVEPEKRLRETVTGDLSGVLETLFHEQHDGTSITFNWYVRLTNPALNLLGYAAERVYR
                     HSHDRVMTSGEKGLSEYCKRRAASGRA"
     gene            3924..>4300
                     /locus_tag="VNT12_02105"
     CDS             3924..>4300
                     /locus_tag="VNT12_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI44936.1"
                     /translation="MPKVTIIGAGLAGSEAAWQAAERGCSVELWEMRPTKETPAHRTE
                     HFAELVCSNSLGNLSLTTASGLLKEELRRLGSVILDRADANSVPAGGALGVAREDFPR
                     AVTATVRAHPNIEVVRKEATYIP"
BASE COUNT          795 a         1344 c         1345 g          816 t
ORIGIN      
        1 gaactctacg gcgaccccga gggcgccatt caagcgatgc aggacgccgt cgatgccgga
       61 gctcccgtcc ccgagaacaa ggcctgggcg tacgtccagc tcggcaacct ccgctttact
      121 accggccaac tcgaggaggc agcgaaggac tacgacctgg ctacgaagac cctgcctaat
      181 tacccgccgg cgctggctgg ccaggcccga atcgccgcgg cgaggggtga cctggaggat
      241 gcagcagccc tctaccagga agccttcgac cgcatgcccc tagccgagta cgctattctc
      301 ctgggcgacg tctacgccga gatgggcgaa aagcagaagg cggccgagca gtacgatctc
      361 gtccgggtga ttgacaagct gtatagggcg aacggggtca acacagacct ggagatagcg
      421 ctcttctacg cagaccacga catgaagctg cagacctcgc tcgaaaaagc gcgctcagcc
      481 tacgatgcgc ggccgagcat ccacgccgcc gacgccctgg cctggtcgct ctacaagaca
      541 gggaactaca aggaggcgaa gaagtattct tcagaagccc ttaagctggg aacgcgcgac
      601 cccctgaagc tcttccacgc cggcatgatc gcaaaggcac tcggccggag agagcaggcc
      661 cgcgagtatc tgcaacaggc cgttgacctg aacccgcact tctctttgtt gtacagcgac
      721 gaagctgttg acagcttgaa agcgctgaac gcgaaggggg aagactgatg ttccgctgga
      781 gttggctgtt ggcgtgtctc gcgtttgccg tgttcgcctc gctgatccca cgcgaggcgc
      841 aggcacatcc cctgggcaac tttacgatca accactacag tcggctcgag ttcgccgatg
      901 agaaggcacg tatcgactac gtgttggact tcgccgagat cccgaccttc cagcagaagg
      961 ggaaactcga ccctaatgag gacggaaagc tctccgacgc ggaggccagg gcctacctgg
     1021 atgccaagct accttctctt gtagagaatc tccatttaag ggttggtaag gaggatctcc
     1081 tcttgcgggt gctcgatagt tcggcggagt acaggccggg gcagggtggg ttgcctatcc
     1141 tccgcatcga ggctcgcctc ctggccgacc ttccgaaaga ctgggaggat cacggcgtcg
     1201 gccattacgc agaccgcacc tacgagaatc gtctgggctg gcgtgagata gtcgtcaagg
     1261 gcggacctgg ggtcgccatc aagggttcca cggcgccgtc gtccggtgtg tcagacgaac
     1321 tgcgtaagta tccgaatgat atgctctcca gccccctgga tgttcgccag gcgaccttca
     1381 cgctcgtccc cggtgagggg tcctttgggg atggcccagt aggtgaggcg gcccgcaact
     1441 atagcacgag cgactacagc ttcaagtcga acggttacat tagcaagctc tatgcctggt
     1501 taaactcgct catttcgttc accagcccct caccgacggt gatcctcatc tcgcttctgg
     1561 ccgcgctgtt gtggggtgcc ttacatgcct ttacgcctgg ccacggcaag acgatcgtgg
     1621 cggcctatct gatcggggat cggggaacgg cccggcacgc ggcctttctc gggttgacgg
     1681 tgacgctgac ccataccctg ggagtcttcg cgcttggggg agtggccatt tacctgtccc
     1741 gctacatatt gcctgaggcg ctcttcccct ggctgagcgt tgtctccggg ttgctagtgg
     1801 tcgtaatagg tctctcgctc ctcttcagcc gctcacgcgg cttgttcggt accggcagcg
     1861 ggaaagcgcg gcacacaaaa ttgaggcatg cggatgggcc ccacacgcat tcgcacgacg
     1921 gccacgacca ctcctatgcg tacgcccgcg tcgcacactc acattcccac ggtgatcacg
     1981 aacactccca tgatcacgtt cactccgcac actcgcattc gcatggcgac cacgagcaca
     2041 cccactcgca cggcggccac acgcacactc acctgccacc gggagcggat ggttcgaagg
     2101 cgggtatacg tagcctggtg gccctcggcg tcagcggtgg tctggtaccg tgtccggcgg
     2161 cgcttgtcct cctgctcagt gcgatctcgc taggtcgact ggggtttggc atggtactcg
     2221 tcgtcgtgtt cagcatgggg ttggccgtcg tactgaccgg cattggcctg ctaatggtct
     2281 acgcgaggcg gtcgttcgag aggtactcgt tcgaggcacg cataccgctc ttcttgcctg
     2341 tcgcgagcgc tgctgtgatc tcgttcgtcg ggctcgtgat cgtgctcggc gccatcagcc
     2401 agaccgggct cctttaaccg atgcccagct tgttacactg gttctgaggc gccggattcc
     2461 ggcggggtca ggagcaatta caggacgtct cgggaaggag agtagcgtga agccggaact
     2521 agtggacgaa ctgggggtgg acttctacga ccgcttctac cacccggaag ttctgtccga
     2581 gacacaacgc cggcacgcga accgctggca cgatctggag gcgcagacat gaaccgcagc
     2641 aacgtcgcat gggttctgcg ggccgacggg ctcttcaacc tgttcgccgg actcgtactg
     2701 ctcaccttct acaggcccgt tgtagccttg atcggctggc cgaatacgca gatgcccatc
     2761 tacgccaacg tgctcggtgc ggccttgatc gggctgtcgt tgaccgtgat actcgtctcc
     2821 cgccatcccg agcagtcgcg tggcatcatc ctctcgggcg tcctaaccaa ggcgctggcc
     2881 ggcgtgacga tcctctactg ggtcttcata gcagggatag atctaccctc cccctggctc
     2941 ctgcccgcgg cggtaggagt gcaggttctg ttcgcccttg gcgaagccgc atactgcctg
     3001 ccctccagaa ccggtgggcc gaataggagg gtcgcctgat gaaagcggga acaggtgccg
     3061 gtatcgcctg tgctatcggc gtggcggggg gtatcgtgat cggggctcgc gccgtcaaga
     3121 tctacgactg gcgcaccgaa tggaccattc ccgcgccgat gcccatcgtc tacgaggcca
     3181 tgacctcgcg cgaggcggta cgtgagtggt ggccggacat ggaactcgtc gatgacagcg
     3241 gagatgatga cctccacgtt ggcagcaccg tctctttccg ggtgcaccag gctcccgatg
     3301 tcgcacgcct cgctccgccc ttccgcatcc attgcctata caccgacgta gaaccggaaa
     3361 agaggctgag ggagacggtg accggagacc tgagcggtgt gctagagact ctgttccacg
     3421 agcagcacga cggtacctca ataactttca actggtacgt gcgcctgacc aacccggctc
     3481 tcaacctcct ggggtacgcc gccgaacgcg tgtaccgcca cagccacgac agggtgatga
     3541 cctcgggcga gaaggggctc tcagagtact gcaagcggcg ggcagcctcc gggcgcgcgt
     3601 aggtagtgga tcccaagaca acctcaacca cggaaggggt gtaccgaatg acaacccagc
     3661 cagtcgagaa cgtggccgct ccgctaaggc ctacatcttt cgagaggttc gctgggatgt
     3721 gtggggtcct tgccggtgtc gccggttttc tgtacgcggt ctctttcgtc tcgtagaaag
     3781 gcgagccggt gatgggggcg gtgcttgccc tgccggctgg ggtatagacg gtggcccggc
     3841 tcggaatggc accttcagga gcgcgggttt gacttagcgt agaataacct ccctgcaaag
     3901 ccacgagcgg aggatcttct ttgatgccga aagtgaccat catcggtgcc ggactggccg
     3961 gaagcgaggc ggcctggcag gcggcggagc gcggctgctc ggtggagctg tgggagatgc
     4021 gccccacgaa ggagacgccg gcccaccgca cggagcactt cgccgagctg gtctgctcga
     4081 actccctggg caatctttcg ttgaccaccg catcggggct cctcaaggag gagcttcgcc
     4141 ggctcgggtc cgtgatcctg gaccgggctg atgcgaactc tgtcccagcc ggcggcgccc
     4201 tcggggtggc ccgcgaggac ttcccgcgcg ctgtcaccgc gacggtacgc gcccacccca
     4261 acatcgaggt cgtgcgcaag gaggccacat acatccccga
//
LOCUS       DATMJA010000094         7896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_545647, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000094 DATMJA010000000
VERSION     DATMJA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545647"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1946
                     /locus_tag="VNT12_02110"
     CDS             222..1946
                     /locus_tag="VNT12_02110"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWI44937.1"
                     /translation="MRVALAQINTTVGDIWGNVEKMADTLERATDSGAELVAYPELTI
                     PGYPPEDLLMRQSFIEENMRALHEFAGRVPENVVAAAGFVDLGTDLYNACALVSGGEV
                     LYRYHKRYLPNYGVFDENRYFREGTGASIFDLGDALVGVSVCEDIWYPGGPAREQALG
                     GASVLLNISASPYHRRKGAARERMLGVRASDYGCYVLFCNLTGGQDELVFDGHSVVFD
                     PEGRLVARASQFKEDLLIVDLYPGHSLMQRLHDPRPRKENTEHTPEIVKVPRHDPAAA
                     EPVEPRIEPSLPEEGEVLEALVLGLGDYFRKNGFSRAVLGLSGGIDSSLAAAVAVEAL
                     GPQNITGVLMPSRYTSEGSNTDAAALAKNLGIDTQTIPIGPAFDAYREMMEGAFKGLP
                     EDVTEENLQSRIRGNILMALSNKFGWIVLSTGNKSEMSVGYSTLYGDSAGGFAVIKDV
                     PKNLVYRVARHFNERKDREVIPDSVLSKEPTAELRENQRDTDSLPPYEVLDPILEAYI
                     EEDRGVAEIVASGFEEDDVRRIVQLVDRAEYKRRQAPTGIKVTGRAFGRDRRMPITNR
                     YFERNHKE"
     gene            complement(2068..3057)
                     /locus_tag="VNT12_02115"
     CDS             complement(2068..3057)
                     /locus_tag="VNT12_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44938.1"
                     /translation="MAPQDVAYAISYSLLVGAMLHLVALLLADAAYLGLRSVGPYQSG
                     NWPEMLWALGMILLGLTSTTSPEVPADPNVRRIQPWQIILYWLGPLSPPIHFTILLLW
                     GAMHPPLPAYVLVGCAMLLLYMALRIGLVSSVSKHLGQEREESARVKEQGRLLYELHD
                     TAKQEVHSISLTLRAALDTERRGEREDALEMFRRALAASREAEFRISRPYDELSALRG
                     GSPPGAADFLRLRKFEEYFRVKAHDDIQASLEVLNPAETAALLRVFVEAFWNVAKHSG
                     ASNLYLESRRVGSVLIIRVRDDGRGFDTAKPSPGLGLEYMRRRAGEVGADIWT"
     gene            3509..3709
                     /locus_tag="VNT12_02120"
     CDS             3509..3709
                     /locus_tag="VNT12_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI44939.1"
                     /translation="MEYARLGNTGLMVSELCLGCMTFGQEADEETSTEIVGRFLDAGG
                     NFLDTADVYSKGVSEEITGRAL"
     gene            3785..4138
                     /locus_tag="VNT12_02125"
     CDS             3785..4138
                     /locus_tag="VNT12_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44940.1"
                     /translation="MDSYDFDVCLTPAARELLRTGEALVLDWHRMAICCAGAGEASLY
                     AASEEDLSRRKGLVRLKGEDPIYAARAIFPHLAGREIKLDARKTFGIRRFSSDLPGDF
                     GLRAVMGYLPESTAR"
     gene            complement(4140..4376)
                     /locus_tag="VNT12_02130"
     CDS             complement(4140..4376)
                     /locus_tag="VNT12_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44941.1"
                     /translation="MGQCEVCGNDYDKAFSVTVSGGDSHTFDSFECAIHALAPACEYC
                     NVKVIGHGIEAAGSFYCCAHCARMAGAEGVSNRV"
     gene            4536..5525
                     /locus_tag="VNT12_02135"
     CDS             4536..5525
                     /locus_tag="VNT12_02135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI44942.1"
                     /translation="MDQRHLGSEGLVVSELGLGCMGMSEFYGTGDEEESIATIHRAID
                     LGITFLDTADMYGPFTNEKLVGKAISGRRDEVVLATKFGNVRGEDGSFRGVSGMPDYV
                     RKACDASLLRLGVDHIDLYYQHRVDPETPIEETVGAMKELVEAGKVRYLGLSEAAAET
                     IRKAHAVHPISALQSEYSLFTREVEDEILPTVRELGIGFVPYSPLGRGFLTGRWRSIE
                     DMPENDTRSARFPRFAEENFEKNLVLADKVREVAEGKGITPGQLALAWLLVQGDDIVP
                     IPGTKRRQYLEDNAGAAGVTLTEDDLASIEEAMPRGSAAGERYSEVQMRTIDC"
     gene            5535..6134
                     /locus_tag="VNT12_02140"
     CDS             5535..6134
                     /locus_tag="VNT12_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565683.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HWI44943.1"
                     /translation="MTDVEHISRKGTGVPISWFPIPEESELPDDLQGLFRKAREMVGF
                     VPNVFRAYSYRPERLSAWFAHYRQLHEPTPNLDAAEREMIAVAVSMANGCLYCLVAHG
                     AALRQALGDPILADRITLDHRRAGLDERRRAILDFAVKVTERPLDCDPEDLERLKGFG
                     LTEEEVWDVIEVASMYNFTNRMSLACGMIPNEEYHALAR"
     gene            6157..6411
                     /locus_tag="VNT12_02145"
     CDS             6157..6411
                     /locus_tag="VNT12_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44944.1"
                     /translation="MLQVISVLGALAILGAFAANLFGWIQPSNLWYSVANFLGAAVLT
                     VVAVIDQQLGFLLLEGTWALVSLWGIFSTLRGRQVPPGAH"
     gene            6457..6657
                     /locus_tag="VNT12_02150"
     CDS             6457..6657
                     /locus_tag="VNT12_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44945.1"
                     /translation="MSGVKYVSEVKVEPVEGKIRHAYLPVQDDPVVFGVHSEVAEHYG
                     VPPEDVEPYDTTLDYVVAAAGG"
     gene            6658..6921
                     /locus_tag="VNT12_02155"
     CDS             6658..6921
                     /locus_tag="VNT12_02155"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWI44946.1"
                     /translation="MLGTFSGALAARQVSFENLHAETIGEIEVESKVLVIKRIKQTFH
                     LTADESDRETIERVLEVYADRCPVAASVKGSIEISSELDLSVA"
     gene            complement(6935..>7896)
                     /locus_tag="VNT12_02160"
     CDS             complement(6935..>7896)
                     /locus_tag="VNT12_02160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI44947.1"
                     /translation="ESFELSRCVYRSYGYSYDWDYIYYPDRIRELQEQGLMISCVAVT
                     SEGEFVGHLAITVERPGSPVGEAGQAVVDPRFRGHHLFPKMKTFMAQWAGDAGMYGLY
                     SEATAVHPYSQRGNLHLGARETGFLLGYIPASVSYKQIGDERADRRGSVALFYMRVRD
                     EPEREIYPPVPYLEVVRRVIEHNGLRRVIGEVPDPTLQPSRMSVEVRQDHNLAFLRVE
                     EPGADLEELVRSHLRDLSLHRVDCVYVDLPLSHPATPIAAAELNNLGMFFGGIIPEAH
                     PGGGDVLRLQYLNNVEIEAGDVSTASDFGEELLGLIFSQHTLP"
BASE COUNT         1529 a         2478 c         2525 g         1364 t
ORIGIN      
        1 agcgccaggt cctttaccac ttcgagccgc agcgagtttt cggtgtgcga cccgcaagcg
       61 tctacgagaa gacctgcggc cagggctcct gcatccgcgg gggtgcgctt tcgggctggc
      121 atcgcgtggg attctatccg aaggtcggcc tggccggggt gacgctctac aggcatgagc
      181 atcggccggc acgaaccctc caggcgcgat atatttagtg catgagggtt gcgctggccc
      241 agatcaacac gacggtcgga gatatctggg ggaatgtcga gaagatggcc gacaccctgg
      301 agcgggcgac cgattcgggt gctgagctcg tcgcataccc cgagctgacc atccctggct
      361 atccgcccga ggatctcctc atgcgccaga gctttatcga ggagaacatg agagcgctcc
      421 acgagttcgc cgggcgggta cccgagaacg tggtggccgc cgcgggcttc gttgacctgg
      481 gcaccgacct gtacaatgcc tgcgccctgg tgtccggtgg cgaggtactt taccgttacc
      541 acaagcgtta cctaccgaac tacggcgtct tcgacgagaa ccgctacttc agggagggca
      601 ctggcgcctc gatcttcgat ctgggcgacg ccctcgttgg ggtgagcgtg tgcgaggaca
      661 tctggtaccc cggtggcccg gcccgcgagc aggccctcgg cggggctagc gtcctcctca
      721 acatctcggc ctccccttac cacaggcgga agggcgccgc tagggaacgg atgttgggcg
      781 tacgggcttc ggactacggt tgctacgtgc tcttctgcaa cctcacgggc ggccaggacg
      841 agctcgtctt cgacggccac tcggtcgtct tcgaccccga gggcaggctc gtggcgcggg
      901 ccagccagtt caaggaagat ctgctgatcg tggatttgta cccgggtcac tcgctcatgc
      961 aacgcctcca cgaccccagg ccgcgcaagg agaacaccga gcatacaccc gagattgtaa
     1021 aggttcctcg acacgacccg gctgctgcgg aaccggtcga acccaggatc gaaccttccc
     1081 taccggagga aggcgaggtg ctagaagccc tcgtgctggg cctcggagac tacttccgca
     1141 agaacgggtt ctcgcgggcc gtgctcggtc tctcgggcgg catagattcg tctctggccg
     1201 ccgccgtcgc ggtcgaggcc cttggtcccc agaacataac gggcgtcctc atgccgagcc
     1261 gttacacctc ggaagggagc aataccgacg ccgcggcgct cgccaagaac ctcggtatag
     1321 atacccagac catccccatc ggtccggcct tcgacgccta cagggagatg atggagggcg
     1381 ccttcaaggg gctccccgag gacgtgacgg aggagaatct gcagagccgc atcaggggga
     1441 acatcctgat ggcactctcc aacaagttcg gttggatcgt cctgtctacg ggcaacaaga
     1501 gcgagatgag cgtgggctac tctaccctct acggcgactc cgcgggtggt ttcgcggtca
     1561 taaaggacgt gccgaagaac ctggtctaca gggtggctcg gcacttcaac gagaggaagg
     1621 atcgtgaggt cattcccgac tcggtcctct ccaaggagcc caccgccgag ctgcgcgaga
     1681 accagcgcga cacggactcc ctgccaccct acgaggtcct ggatcccatc ctcgaagcct
     1741 acatcgagga ggataggggt gtcgctgaga tcgtagcctc aggcttcgag gaggacgatg
     1801 tgcgtcgcat cgtgcaactc gtggacaggg ccgagtacaa acgccgccag gccccgacag
     1861 gcataaaggt cacgggccgg gccttcggcc gcgacaggcg tatgcccatc accaaccgtt
     1921 acttcgaacg caaccacaaa gaatagctct acagctacat tctgctcggc ctcgtgaagc
     1981 ctgtctaatc gcccggccac gggttggcgg tgttctggcg cttgcggaac cttacctgca
     2041 cagaagtacc gcgtccaggg gtggagatca cgtccagata tccgcgccga cctctccggc
     2101 acgtcggcgc atgtactcta gaccgaggcc tggcgacggt tttgccgtgt cgaagccgcg
     2161 gccgtcgtcc cgaacacgga tgatgagcac tgagcctacc cgacgagact ccaggtacag
     2221 gttgctcgcg ccggagtgct tggcgacgtt ccagaacgcc tccacgaaca ccctgagaag
     2281 ggcagcggtc tcggccgggt tcaatacctc gagggaggcc tggatgtcgt cgtgggcctt
     2341 caccctgaag tattcctcga acttgcgcag gcggaggaag tctgcggccc ctggcgggct
     2401 cccgccgcgc agggcagaaa gctcgtcgta ggggcgtgag atccggaact cggcctccct
     2461 cgatgccgcc agggctctcc tgaacatctc cagcgcatcc tcacgttctc cgcgacgctc
     2521 ggtgtcgagg gcggccctca gagtcagcga gatgctgtgc acttcctgct ttgccgtgtc
     2581 atgtagctcg tagagaaggc ggccctgctc ctttacacgg gccgattcct cccgctcctg
     2641 gccgaggtgt ttgctaaccg acgagaccag cccgatcctc agggccatgt acaggaggag
     2701 catggcgcat ccaacgagca cgtaagccgg cagcggcgga tgcatcgcgc cccagaggag
     2761 caggatcgtg aagtggatcg gcggggagag cgggccgagc cagtacaaga ttatctgcca
     2821 gggctgtatc cgcctgacgt tcgggtcggc aggaacctca ggagaggtcg tcgacgtgag
     2881 gccgagcagt atcatgccta gagcccacaa catctctggc cagttgccgc tctggtacgg
     2941 cccgacggag cgaagtccca ggtaggcggc atcggcgagg agcagcgcca cgaggtgaag
     3001 catcgcccct accagcaacg agtatgagat ggcgtaggcg acgtcctgcg gggccaccgg
     3061 cgcggcgaag ccaaactaca agtgttgcga cgcgctccag agcgagttcc ctgcgagccg
     3121 gcagaccatg cccagtccca tgagccgcag gaaggcccgc tcgtggccct ccgcgcgcgc
     3181 cgcggcgcgc agcaggatca cgggcggtga aggcggccgt agacagggag aagaccgtag
     3241 cgattacgga ttttgccccc gagccctggg gcaaaaacgc gaagacgagc gcgcccagag
     3301 cccagcaggc cagaacggct gtcggaggga tgcgcgggac gcgaggatcg cgcaacctac
     3361 ccgcatccga accttgcaac tctcaaccct ccagaactcg aaccaaccaa tcctaccacc
     3421 ctcgtgcctt gcgtggccag cgaggtcggt cagacctcgt atgctaaagt acggcctttc
     3481 ggtggcagag atccgagagg agcagcctat ggagtacgca cgtcttggga acacagggct
     3541 gatggtctct gagctatgtc tggggtgcat gaccttcgga caggaggccg acgaggagac
     3601 ctctacggaa atcgtcggcc ggtttctcga tgccggaggc aacttcttgg acactgccga
     3661 cgtctactcg aaaggggtct ccgaggagat cacaggcagg gcgctctgag gcgtacgcga
     3721 cgaggagccg gcgccgttcg tgtaagggcg cttcgcgaag cgcccctact ggtaaaatgt
     3781 tggcatggac tcctatgatt tcgacgtttg cctgacgccg gcggcgcggg aactgctgcg
     3841 tacgggcgag gcgctcgttc tcgattggca caggatggcg atctgctgcg cgggggcggg
     3901 agaggccagc ctctacgccg ccagcgagga ggatctcagc aggcgcaaag gcctcgtccg
     3961 cctgaagggt gaggacccga tctacgccgc ccgcgccatc ttcccgcacc tcgccggacg
     4021 cgagataaag ctcgacgccc gcaagacctt cggtatccgc cgcttctcct cggacctgcc
     4081 gggagatttc ggactgcgcg ccgttatggg ctacctgccg gagagcacgg cgcgctagct
     4141 cagacccggt tcgaaacgcc ttccgcgcct gccatgcgcg cgcagtgggc gcagcagtaa
     4201 aatgagccag ccgcctcgat gccgtgcccg atcaccttga cgttgcagta ctcgcaggct
     4261 ggggcgagcg cgtggatcgc acactcgaag ctgtcgaagg tgtgcgagtc cccaccggac
     4321 accgtgacgg agaacgcctt gtcgtagtcg ttgccgcaga cttcacattg gcccacagct
     4381 ccactccttt ccgacgtttc tctttcgtag atcatgcccg gtgccggagg atcgcaatct
     4441 gcccaggggc gtatccgtcg cgcggcgtgc tcgcgccgat tgtgtaataa actcgatcgg
     4501 tagtatccga gaacgccagg ctagaggaga gacctgtgga tcagcgacat ctgggtagtg
     4561 aagggctcgt cgtgtcggag ctagggctcg ggtgcatggg aatgagcgag ttctacggca
     4621 cgggcgatga ggaggagtcc atagccacca tccaccgcgc catagatctg ggcataacct
     4681 ttctcgacac ggcggacatg tacggtccgt tcaccaacga gaagctcgtc ggcaaagcca
     4741 tatccggccg cagggacgag gtcgtgctcg ccaccaaatt cggcaacgtg cgcggggaag
     4801 atgggagctt ccgcggcgtg agcggtatgc ccgactacgt gaggaaggcc tgcgacgcgt
     4861 cgctcttacg cctcggggtc gaccacatcg acctctacta ccagcaccgc gtggacccgg
     4921 agacgcccat cgaggagacg gtcggcgcca tgaaggagct tgtcgaggcc ggcaaggtca
     4981 gatatctagg actctccgaa gcggcggcag aaaccatcag gaaggcccat gccgtgcacc
     5041 ccatcagcgc cctccagtcg gagtattcgc tctttacccg cgaggtggag gacgagatcc
     5101 taccgaccgt gcgcgaactg ggcatcgggt tcgtccccta cagcccgctc gggcgaggct
     5161 tcctcaccgg acgctggagg agcatagagg acatgcccga gaacgacacc cgcagcgccc
     5221 gcttcccacg cttcgcggag gagaacttcg aaaagaacct ggtactggcg gacaaggtgc
     5281 gagaggtcgc cgaaggcaag gggatcaccc ctggacaact ggccctcgct tggctgctcg
     5341 tccaggggga tgacatcgtc cccatcccag gaaccaagcg ccgccagtac ctcgaagaca
     5401 atgctggcgc cgccggtgta acactcaccg aagacgacct cgccagcatc gaggaggcca
     5461 tgcctcgcgg ctcggccgcc ggcgagcgtt acagcgaagt gcagatgcgg accatagact
     5521 gctaggagga gtcgatgacc gatgtcgagc acatatcgcg caagggaacg ggtgtgccca
     5581 ttagctggtt ccccatcccc gaggagtccg agcttcccga tgacttgcag gggcttttca
     5641 ggaaggcgcg ggagatggtc gggttcgtcc ccaacgtctt cagggcttac agctaccgtc
     5701 ccgagcggct ctccgcctgg ttcgcccact accgccagct ccacgagcca accccgaacc
     5761 tggacgcggc ggagagggag atgatcgccg tggccgtctc gatggcgaac ggctgcctgt
     5821 actgcctggt cgcccacggc gccgcccttc gccaggccct gggcgaccct atcctcgccg
     5881 accgcatcac cctggatcac aggagggccg gcctcgacga gcgccgcaga gccatcctgg
     5941 acttcgccgt caaggtcacc gagcgcccgc tggattgcga tccggaagac ctcgaacgcc
     6001 tcaagggatt cgggctgacc gaggaagaag tctgggatgt aatagaagta gcaagcatgt
     6061 acaacttcac caaccgtatg tcgctggcat gtggcatgat ccccaacgag gagtatcacg
     6121 ccctggcccg ctagagggcc gtccgtggag gtgcttgtgc tgcaggtgat ttcggttctc
     6181 ggcgcgctgg ctatcctcgg cgccttcgcc gccaacctgt ttggttggat acagccctcc
     6241 aacctctggt actcggtagc caacttcctg ggcgcggccg tgctgacggt ggttgctgtc
     6301 atagaccagc aactgggctt cctgcttctg gaaggcacct gggccttggt gagcctctgg
     6361 ggcatcttct ccaccctgcg cgggaggcag gtgccaccag gtgcgcacta gagagggact
     6421 tgtatactgc ggacatacag aggagaggag aatagcgtgt caggagtaaa gtacgtctcg
     6481 gaggtgaagg tagagccggt ggagggtaag atccgccacg cgtatctccc cgtccaggat
     6541 gatccggtag tgttcggcgt ccacagcgag gtcgccgaac actacggcgt ccccccggag
     6601 gacgtcgaac cctacgacac cacgctcgac tacgtggtcg cagccgcggg cggctgactg
     6661 ctcggcacct tttcgggcgc gctggcagcg cgtcaggtgt cattcgagaa cctccacgcc
     6721 gagacgatcg gagagatcga ggtcgagagc aaagtgctcg tcatcaagcg catcaaacag
     6781 accttccacc ttaccgccga cgaaagcgac cgcgagacca tagaaagggt gctcgaggtc
     6841 tacgcagatc gctgcccggt ggcggcgtca gtaaagggga gtatcgagat ctccagcgag
     6901 ttggacctct cggtcgcctg aaccgcctgc gagcttaagg aagtgtgtgc tggctgaaga
     6961 tcaagccaag gagctcttct ccgaagtccg aggctgtgct gacgtcgccg gcttcgatct
     7021 cgacgttgtt caggtactgg aggcggagca catctcctcc tccggggtgg gcttcgggga
     7081 tgatcccacc gaagaacatc ccgaggttat tcagctcggc cgcggcgatc ggtgtcgctg
     7141 gatgggagag aggaagatcc acgtagacgc aatccacgcg gtgcagggag agatcccgta
     7201 gatgactgcg gacgagctcc tccaggtcgg cccctggctc ctcgacccgc aggaaggcga
     7261 ggttgtggtc ctgacgcacc tccacgctca tccgggaggg ctgtagcgtc gggtcaggga
     7321 cctcgccgat cacccgtcgg agcccgttgt gctcgatgac gcgcctgacc acttccagat
     7381 aaggcacagg ggggtagatc tcccgctccg gttcgtctct gacgcgcatg tagaacaggg
     7441 cgacggaccc gcgacgatct gctctctcat cgccgatctg cttgtacgag acagaggcgg
     7501 ggatatagcc gagcaagaac cctgtctccc tcgcgccgag atgtagattg cctctctggc
     7561 tgtacggatg cacggctgtc gcctcgctgt agagcccgta catgccggcg tcaccggccc
     7621 actgtgccat aaaggttttc atcttcggga acagatggtg tccacgaaag cgcgggtcca
     7681 ctaccgcctg cccggcctcc ccaacaggcg agccgggacg ttccacggta attgccagat
     7741 gccccacgaa ctcgccctca gacgttaccg cgacgcaaga gatcatgaga ccctgctcct
     7801 gaagctccct gatcctgtca ggatagtaga tgtagtccca gtcgtagctg tacccatagg
     7861 agcggtagac gcagcgcgaa agctcgaagg attcct
//
LOCUS       DATMJA010000095         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_604859, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000095 DATMJA010000000
VERSION     DATMJA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604859"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VNT12_02165"
     CDS             complement(<1..1106)
                     /locus_tag="VNT12_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HWI44948.1"
                     /translation="MLTTLYAYFGEGVAMGRFDTIVLGGQAVLPGQGLIRADVGIKDG
                     RVSAIGESLSSGDADEVVDAAGKLVLPGAVDAHFHLGIYRDISEDTRSETLSSLAGGV
                     TSIISYFRTGSHYLGKSGPYAEIFPEVLEATSGHSRVDYGYHLAPMVRDQVAEIGRLA
                     SEDGVTSFKYYMFYKGLDLSGASSDASGYTMSENYDLGHLFEIMEQVAKAQSESDSRV
                     SLSIHCEQPELIRIFSERVKQEGVLSGLEAYSASRPTLTEHLAVAEVGVLAGHTRCPI
                     NLLHLSSKEAYEAALELKALDPYLDVRLETTLHHLALTYENYKDQRGKVNPPIRAQTD
                     VDALWRGVARGEIDWICSDHACCSEEHKEGDLWQA"
     gene            1207..>2066
                     /locus_tag="VNT12_02170"
     CDS             1207..>2066
                     /locus_tag="VNT12_02170"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI44949.1"
                     /translation="MNGKQALEGIKIIDAATLFAGPLAATVLGDFGAEVIKVEHPKGD
                     PSRSHGYSKNGVGLWWKMLGRNKRCVTLNLSKPEGQGIFERLASEADVVIESFRPGTL
                     ERWNLGYERLSEINPGLVLARVTGFGQFGPYKNRPGFGTLAESMSGFAHVTGQPDGPP
                     TLPPFGLADGIAALATAVAILTALRARGAGGRGQVIDLAIIEPIFTVLGPQPIAYDQL
                     GVVQERTGNRSNNNAPRNTYQTKDGKWVAISTSAQNIAERVMRLVGRPEFIEEPWFQK
                     GSERAKHADEL"
BASE COUNT          386 a          652 c          666 g          362 t
ORIGIN      
        1 gcctgccaga ggtcaccctc tttgtgctcc tccgaacagc aggcgtggtc ggagcagatc
       61 caatcgatct caccgcgtgc cacaccgcgc cagagcgcat ccacatctgt ctgggccctt
      121 atgggcgggt ttaccttgcc gcgctggtct ttgtagttct cataggtgag cgcgaggtgg
      181 tggagcgtcg tctccaggcg aacatcgagg taaggatcaa gagcctttag ctccagcgcc
      241 gcctcgtagg cctccttgct ggagaggtgg aggaggttta tggggcagcg cgtgtggccg
      301 gcgaggacgc ctacctctgc gacggcgaga tgttcggtca acgtcgggcg cgaggcgctg
      361 taggcttcga ggcctgagag gacgccttct tgcttgaccc tctctgagaa gatccggatc
      421 agctcgggct gctcgcagtg gatagagagt gagacccgcg agtcgctttc gctctgcgct
      481 ttcgccacct gctccatgat ctcgaagagg tggccgaggt cgtagttctc gctcatcgtg
      541 tagccgctgg cgtcgctcga ggcgcccgaa aggtcgagac ccttgtagaa catgtagtac
      601 ttgaaggagg tgacgccgtc ttcggaggcg aggcgaccga tctcggcgac ctgatcccgg
      661 accatcggcg ccaggtgata cccgtaatcc acccgcgagt gcccactcgt cgcctccagg
      721 acctcgggga agatctccgc gtaaggacca ctctttccga gatagtggct gcccgtcctg
      781 aagtagctga tgatgctcgt gacgccaccc gcgagcgaag agagcgtctc gctgcgcgta
      841 tcctccgaga tatcgcggta gatgccaaga tggaagtggg cgtccaccgc accagggagt
      901 acgagtttcc ccgcggcgtc tacgacctcg tccgcgtcgc ctgaagacag gctctcccct
      961 atggccgaga cacgtccgtc cttgatgccc acgtccgccc tgattaggcc ctgtcccggc
     1021 agaacggcct gcccgcccag aacgatggta tcgaatcggc ccatcgccac cccttccccg
     1081 aagtacgcat agagcgtcgt caacatagca cggtgcgcgt ggcccggtca aaaccttgcg
     1141 gtgaaacgat gtatgagcaa agatgagcgc gtagtgttgc gggatttccg ggcaggaggg
     1201 cgtggtttga acggaaagca ggcgctcgag gggatcaaga tcatagacgc ggccacgctc
     1261 ttcgccggac cgctcgccgc gacggtgctc ggcgacttcg gggccgaagt cataaaggtc
     1321 gaacatccta aaggagaccc ctcgcgctcg cacggctact cgaagaacgg cgtggggctc
     1381 tggtggaaga tgctcgggcg caacaagagg tgcgtgaccc tgaacctgag taaaccagag
     1441 ggacagggga tcttcgagcg cctcgcaagt gaggcggacg ttgtgatcga gagtttcagg
     1501 ccaggcaccc tggagcggtg gaacctcggc tacgaacgcc tcagcgagat aaaccctggt
     1561 ctcgtcctcg cccgcgtcac cggcttcggc cagttcggcc cgtacaagaa caggccaggc
     1621 ttcggcaccc tggctgagtc catgagcggc ttcgcccacg tcaccggcca gcctgacggg
     1681 ccgccgacgc tcccgccgtt cgggctggcc gacggcatcg cggcgctggc gaccgcggtc
     1741 gccatcctga ccgccctcag ggcccgcggc gccggcgggc gcgggcaggt catcgacctg
     1801 gcgatcatcg agccgatctt caccgtcctc ggtccgcagc cgatagccta cgaccagctc
     1861 ggggtagtac aggagaggac cggcaaccgc tccaacaaca acgcgccgcg caacacctac
     1921 cagaccaagg acggcaagtg ggtcgctatc tctacgagcg cccagaatat cgccgagcgg
     1981 gtgatgcgtc tcgttgggcg tccggagttt atagaagaac cgtggttcca gaagggctcc
     2041 gagcgggcca agcacgccga cgagct
//
LOCUS       DATMJA010000096         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_723670, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000096 DATMJA010000000
VERSION     DATMJA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723670"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VNT12_02175"
     CDS             <1..462
                     /locus_tag="VNT12_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI44950.1"
                     /translation="WGTREPELEPIDLSDVVHAAAEMAKPLAESAGLDVTLEGQSTWV
                     VADPALLEEALLIPITNAIQHSDPGKTVRLRVSGASVTVEDEAGGIAEEDLPYLFERF
                     YTGKGSFGGTGLGLPICKDLVEKMDGEISVRSEKGVGTSVRIELPEAEPDA"
     gene            455..1147
                     /locus_tag="VNT12_02180"
     CDS             455..1147
                     /locus_tag="VNT12_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI44951.1"
                     /translation="MPRVLIVEDDDSMRDMVGYALADEGMEIEAVADGESALEVFSSS
                     GPFDLVVLDVMLPGVDGITVCKELTSASDVPVIMLTARDDEMSVVVGLEVGADDYITK
                     PFSHRELVSRVRATLRRRRIDARASSKPERLEFPGLAIDLARRQVVAQGTPVDLTAAQ
                     FDILLLLASHPGWVYGRGQLMETVWGTEFSGDSRAADMQIRNIREKIEPDPKNPRYVL
                     TVRGMGYKFAEI"
     gene            1725..2087
                     /locus_tag="VNT12_02185"
     CDS             1725..2087
                     /locus_tag="VNT12_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44952.1"
                     /translation="MLTIDAGGETVLPVFSFREEVELFLHLQAKGADWWPRETTTVEL
                     ASLLLGLCARVEKVALDPLPGFGERAILGFVSTGRRRFMRYSMGEDTAQPERKKPSGR
                     ETWRSAPCRLRRGLLMAA"
     gene            2137..2280
                     /locus_tag="VNT12_02190"
     CDS             2137..2280
                     /locus_tag="VNT12_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44953.1"
                     /translation="MVFGLGAIVLGIRQVRLREHRSERFIGVGLLLGGAFTAVEGLVR
                     LLF"
BASE COUNT          506 a          788 c          886 g          516 t
ORIGIN      
        1 tggggcacgc gagagccgga gctggaaccg atcgacctct ctgatgtcgt ccatgccgcg
       61 gctgagatgg cgaaacccct ggccgagagc gcggggctcg acgtgactct cgaaggccaa
      121 tcgacgtggg tcgtcgccga tcccgcgttg ctagaggagg cgctcttgat ccccatcaca
      181 aacgctatac agcattcgga tcccggcaag acggtacggc tgcgcgtctc cggagcatcg
      241 gtgaccgtcg aggacgaagc gggaggtata gccgaggagg atctccccta cctcttcgaa
      301 cgcttctaca cggggaaagg ttccttcggt ggcactggtc tcggcctgcc aatttgcaag
      361 gatctcgtgg agaagatgga cggcgagatc tcggtccgct ccgagaaggg cgtcggtacc
      421 tccgtgagga tagagctgcc ggaggccgaa cccgatgcct agggtactca tcgtggagga
      481 cgacgactcc atgcgggaca tggtcggcta cgcgctcgcc gatgaaggga tggagatcga
      541 ggcggtcgcc gacggcgaga gtgcgctcga ggtcttctcc agttcgggac ccttcgacct
      601 agtggtcctg gacgtcatgc tcccaggcgt agacggcatc accgtctgca aggagctgac
      661 ctcggcaagc gacgtcccgg tgatcatgct gaccgcccgt gacgacgaga tgagcgtcgt
      721 ggtgggcctc gaggtcggag ccgacgacta catcaccaaa cccttttccc accgggagtt
      781 ggtgagccgc gtccgcgcga ccctgaggcg ccggcgtatc gacgccaggg catcctctaa
      841 gccggagcgc ctggagtttc cggggttggc gatcgacctg gcgcgtcgcc aggtggtcgc
      901 tcagggcacc cccgtggacc tcacggccgc gcagttcgac atcctcctgc tcctcgcctc
      961 ccaccccggg tgggtatacg gccgggggca gctcatggag accgtgtggg gcaccgagtt
     1021 ctcgggagac tcccgcgcgg cggatatgca gatccgcaac atacgggaga agatagagcc
     1081 tgatcccaag aacccccgct acgtcttgac cgtacgcggt atgggctaca agttcgccga
     1141 gatttagcgc tccccgaacg cccgtagcga ggatgtcaac cgcacttatc ggttcgcctc
     1201 gctcggtggg ctcgatcctc atggtcgcta gcgtgcaagc ttgtcaggtc tcctcgttcc
     1261 gcccggctta gcaggaaggc cgcaaggaag tcgaccgtct tcaccgcgcc taccccgtgg
     1321 gtctaatcct cgcggacctc gggcatacag tgttgatgag catcccagga tcgtcccggc
     1381 gttgaacggc tgcgtagcgg ctaccaagtt atcaaagcac ctgtcagggc ggtgaaacca
     1441 tacggagcac tcagcgcaat gaccacggtc acgtaaaact tccttcacag agtccctaac
     1501 aaagccctta ctcaatcctg agcgccgctt acctggccct tacgaaccgg cggtacgctt
     1561 agtcggagcc gaggggacga gacgttagta gaggcctcga cctcatgtgg ggcggttcgt
     1621 gtgcaattac cgttcccgac taggaggtgg caaaagtgtc gaagaataag gcgactcgac
     1681 gatcattctg gttgatcgtc acatgggcgc acagaaggac ggaggtgttg acaatcgacg
     1741 ctggcggcga gacggtgctc cccgtcttca gcttccggga ggaggtcgag ctcttcctcc
     1801 accttcaggc gaagggagcg gactggtggc caagggagac caccaccgta gagctcgctt
     1861 cgttgctact ggggctctgc gcgcgggttg agaaagtggc actcgatccg ttgccgggtt
     1921 ttggggagag ggcgatcctc gggttcgtga gcacgggtcg aaggcgcttc atgcgctact
     1981 cgatgggtga ggatacggcg cagcctgagc gcaagaagcc gtctggacgc gaaacatggc
     2041 gctccgctcc atgtcgtctc aggaggggcc tgcttatggc ggcttgagca tgcatattgg
     2101 gggaacgtct ggtgcgtaag cgcgacaatc ctgagcatgg tgttcggcct cggggcgatc
     2161 gtacttggga ttcggcaggt aaggctaaga gagcatcgca gcgaacgctt catcggggta
     2221 ggactcttgc tgggtggagc gttcaccgcg gtggagggct tggtgcgcct cttgttctga
     2281 gcggacccct tacgctcagg tgccgctcta ctttttcgca accttgtagt ggtcgtcgct
     2341 cagacgatct tgtcctcctc gatcaggtag gcgtgctcct cgatctcgtg gcttcgagaa
     2401 gacgaccacc tccaagttct ccttccaccc ccaccaccat acattcatga ggtggcttct
     2461 agagtgtgca ttctcacgga agtccgcacg ccgcatggtg cataggtggc tgagtaagac
     2521 gtgaccagca cgggtacgat aacctcgtgt gaggtaaggg cagcgaggag ggtgagtatg
     2581 aacgtcacca tcttcggcgc cgggaatatg gggtgtggca tcggacaccg gctggtgtcc
     2641 ggcggacacg acgtgacgat cgtggacagg gacccggagg aagccgggcg gctggc
//
LOCUS       DATMJA010000097         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_72038, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000097 DATMJA010000000
VERSION     DATMJA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72038"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VNT12_02195"
     CDS             <1..1104
                     /locus_tag="VNT12_02195"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWI44954.1"
                     /translation="FRLCRTSALWFGVAEEVLRLTDETRKHFYKGERVYLEGEEVRFP
                     ELADTLEAVREGGVEIFYEGELARRISAYMLEMGGIITEEDLAEYEAVVREPMSVRYG
                     AGAMYTNGPPSAGGPTLAQMLKVVSAYDLATMPEAEYVRVTAGAMKLALSDRETAYLD
                     GAENSRVAERLTGEEYAAEQRRRIFSAFGSPHTTHLSCVDSEGLAVSITASMGYGSGL
                     VVPGTGIPMNNTLGEPELNPRGFHALAPGERLISNMSPTVVSSEEGGVIALGTPGASR
                     IPTAILQTVINVLDFGMVLEDAVLAPRFHAEGDLFAYEAGARTADLDTYERVLTYETP
                     NMFFGGVNAVRRTPDGRFEAAADPRRSGGTAIA"
     gene            1097..2020
                     /gene="rocF"
                     /locus_tag="VNT12_02200"
     CDS             1097..2020
                     /gene="rocF"
                     /locus_tag="VNT12_02200"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HWI44955.1"
                     /translation="MLENVRRPVGIFGVPMDLGQDRRGVDMGPSAIRYARIEPCLEDL
                     GHKVTDLGNAVVPIPETVTAGEEVKHLRPVMSVCEEVSKRAATIVSQGMFPIFLGGDH
                     SIAIGTVSGVARSTPGIRTGVIWVDAHADFNTPATSPSGNIHGMPLATLTGRGHPDLV
                     GIGSPGASVRTEDVVIIGLRSVDVEEKRLLREAGVRVYTMKDIDAYGAARVVRSAIKG
                     LTDVDRVHLSLDLDAVDPAVAPGVGTPVRGGLTFREAHLLMELVNEAGIISSLDVVEV
                     NPILDVKNGTATLAVELVESLMGRRIIDLPR"
BASE COUNT          421 a          682 c          739 g          370 t
ORIGIN      
        1 ttcaggctgt gcaggacctc ggccctctgg tttggagtcg ccgaggaggt gctgaggctc
       61 accgacgaga cccgcaagca cttctacaaa ggggagcggg tatatctgga gggtgaagag
      121 gtgcggttcc ccgagctcgc cgacacgctg gaggcggtcc gagagggtgg ggtggagatc
      181 ttctacgagg gggagctggc gcgcaggatc tccgcctaca tgctcgagat gggcggcatc
      241 ataaccgaag aggatctcgc cgagtacgag gccgtcgtca gggagccgat gagcgtcagg
      301 tacggcgccg gggccatgta taccaacggc ccgccttccg ccggcggccc gacgctggcc
      361 cagatgctca aggtcgtctc cgcctacgac ctggccacca tgccggaagc agagtatgtg
      421 agggtgacgg ccggggcgat gaagctggcg ctcagcgatc gcgagacggc ctacctcgac
      481 ggggctgaga acagccgggt cgcggagcga ttgacggggg aggagtacgc ggcggagcag
      541 cggcgcagga tcttctccgc ctttggctcg ccgcatacca cacatctctc gtgcgttgat
      601 agtgagggcc tggccgtttc gattacggcc agcatgggct acgggtcggg actagtcgtg
      661 ccaggcacgg gcatccctat gaacaacacg ctaggtgagc ccgagctcaa ccccagaggg
      721 tttcacgcgc tcgcgccggg ggagcgtttg atctccaaca tgagcccgac cgtcgtctcc
      781 tcggaggagg gcggtgtcat cgcgctaggg acgccaggcg cttcgcgcat acctacagca
      841 atactacaga cggtcataaa tgttctggat ttcgggatgg tgctggagga tgcagtcctc
      901 gccccgcgct tccacgcgga gggggatctc ttcgcctacg aggccggtgc ccgcacagcg
      961 gacctcgaca cctacgagcg cgtgctgacc tacgagaccc ccaacatgtt cttcggcggg
     1021 gtcaatgcgg tcaggcgaac accggacgga cgcttcgagg cggcggccga tccccgacgt
     1081 tcgggaggaa cagccattgc ttgaaaacgt acgcaggccg gtgggaatct tcggtgtgcc
     1141 tatggatctc ggccaggacc gacgaggcgt ggacatggga ccaagcgcca tccgttacgc
     1201 ccgcatcgag ccatgcctcg aggacctcgg ccacaaggtt accgacctcg gcaacgcagt
     1261 ggtgcctatc cccgagaccg tcacggcggg agaggaggtc aagcacctcc gtcctgtaat
     1321 gagcgtctgt gaagaggtct ctaaacgggc cgcgaccatc gtctcccagg ggatgttccc
     1381 gatcttcctc ggcggggatc actccatcgc catcgggacc gtctccgggg ttgcgcgctc
     1441 cacgccggga atccgaactg gtgtgatctg ggtcgacgcc cacgcggact tcaatacacc
     1501 tgcgacctcg ccttcgggca acatccacgg catgccgctg gcgaccctaa cggggcgcgg
     1561 ccatcccgat ctcgtgggga tcggaagtcc tggggcgagc gtcaggacag aggacgtcgt
     1621 gatcatcggg ttgcgctccg tggatgtgga agagaagcgc ctgctgcgtg aggccggcgt
     1681 gagggtctat acgatgaagg acatcgacgc ctacggcgcg gcgcgcgtcg tgcgaagcgc
     1741 catcaaaggc ctcacggacg tcgacagggt gcacctctcg ctcgacctcg acgccgttga
     1801 ccccgcagtc gccccaggcg tcggcacacc cgtgcgcgga gggctcacct tcagggaggc
     1861 gcacctcctg atggaactcg tcaacgaggc cggaataatc tcttcgctcg atgtggtcga
     1921 agtcaacccc atcctggatg tcaagaacgg cacggccacg ctcgctgtcg agctagtcga
     1981 gagcctgatg ggacgcagaa tcatcgacct gccacgatga gcggaaggcc ttggccttcc
     2041 gctcatcgtg catgtcagca cgctccggct tcgcctgtgc tttcagcact tcagcaagtt
     2101 gacctgtagc cataccagga gcatagatcc cgaccaccgt ggaaacaggt agctcgctcg
     2161 ccagggcgaa gccgaagagg gagaagctga aatgctgaca aggcgcgaag cg
//
LOCUS       DATMJA010000098         2960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_368480, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000098 DATMJA010000000
VERSION     DATMJA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368480"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VNT12_02205"
     CDS             complement(<1..325)
                     /locus_tag="VNT12_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWI44956.1"
                     /translation="MASGASPTHSQIRWEGHRQAFEDYLQGLRFTTEARLGGLEGAMR
                     YSMLAGGKRVRPTLCMEVAWIFGAEPSRVLPSAAAIELIHTYSLIHDDLPAMDDDDYR
                     RGMPTA"
     gene            complement(309..728)
                     /gene="nusB"
                     /locus_tag="VNT12_02210"
     CDS             complement(309..728)
                     /gene="nusB"
                     /locus_tag="VNT12_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWI44957.1"
                     /translation="MSRRTARKQAFLNLYQSDVNDSPLGPTIERWREYRGDIEEYAIR
                     LARGVKEEQQQLDAVLSDVAVGWPVHRMSAVDRSILRLALYEMLYVDDVPPEVAVNEA
                     VELARGFSGDEAPSFVGGVLRGAELRTQGKVGHGERG"
     gene            complement(746..1303)
                     /gene="efp"
                     /locus_tag="VNT12_02215"
     CDS             complement(746..1303)
                     /gene="efp"
                     /locus_tag="VNT12_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWI44958.1"
                     /translation="MISTNQFRNGTAIRVDGKRFTILNFQHVKPGKGGAFVRTRLRNI
                     DSGAVLEKTFRAGEKLENVRTESRPMTYLYRDGDLLYFMDSDTYDQVPVPFDVVGEAS
                     GYIVEGSTVTVLSADAEVVSVEPPAHVELGVSQTDPGLKGDTATGGSKPATLETGIVV
                     QVPLFINAGDRVRVDTRSNEYLTRV"
     gene            complement(1463..1897)
                     /locus_tag="VNT12_02220"
     CDS             complement(1463..1897)
                     /locus_tag="VNT12_02220"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWI44959.1"
                     /translation="MSKIFVLNGVNLGTLGTRRPDIYGTMTLDDIERLIKDEFPALDL
                     EFRQTDSEGEMVGFTREAAGSDGLIINAGAWTHYSYALHDALEVLDVPKVEVHLSNIH
                     TREEWRRVSVISPVVDALIAGMGAHGYVAAVRYVLEGRSDSP"
     gene            complement(1890..>2960)
                     /gene="aroB"
                     /locus_tag="VNT12_02225"
     CDS             complement(1890..>2960)
                     /gene="aroB"
                     /locus_tag="VNT12_02225"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWI44960.1"
                     /translation="VAVPVTQPYRVFIGAGIDLAAIFSSSLEPGLCVIFTDSTVGPLH
                     AERVQGALERAGWRVAGKIEVPSGEASKSIETYADALRRLARLRLTRDGTIFALGGGV
                     VGDLGGFVAGTYLRGVDLVMLPTSLLAMVDSAVGGKVGVDLPEGKNLVGAFVRPRLVA
                     ADLDWVDTLPEREISNGLAETIKMGLLSGGDFFDDLGLIGAARTGVLDGLETLILHSV
                     RFKSQVVAEDELEGGLRAILNYGHTVGHALEAAAGYNLQHGQAVAAGMLAAASLSRTR
                     FGRDLTRVHEDLISAAGLLRRVPDVGLEQALLAMGRDKKRFSADGPAGHRFVLLEDIG
                     RPVRSVPVSEEEVREAIGTIVE"
BASE COUNT          508 a          989 c          891 g          572 t
ORIGIN      
        1 gggcggtcgg cattccgcgt ctgtagtcgt cgtcgtccat ggcaggcagg tcgtcgtgta
       61 tgagcgagta ggtgtggatg agctctatgg ccgcggcgga gggcaacacg cgagacggct
      121 cggcaccgaa gatccaggcc acctccatgc acagagtggg cctgacgcgc ttgccgccag
      181 cgagcatgga gtacctcatg gccccttcga gtcctccgag ccgtgcctcc gttgtgaacc
      241 tgaggccctg caggtagtcc tcgaaggctt gcctgtgccc ttcccacctg atctggctgt
      301 gcgtagggct agccccgctc gccatgccca accttgccct gcgttcttag ctcagcgcct
      361 ctcagaacac ctcccacgaa ggacggcgct tcatcgccgg agaatcccct ggcgagctcg
      421 acggcctcgt ttaccgcgac ctcgggaggg acgtcatcca cgtagagcat ctcgtagagc
      481 gccaggcgga ggatcgaacg gtccaccgcg ctcatgcggt gcaccggcca ccccacggcg
      541 acgtcggaga gcaccgcgtc gagctgttgt tgctcctctt ttacaccgcg ggcgagcctt
      601 atagcatact cctcgatgtc ccccctgtac tcccgccagc gctctatggt cgggccgaga
      661 gggctgtcgt ttacgtcgct ctggtacagg ttcagaaacg cctgcttgcg ggccgtccgg
      721 cggctcaagg gtctctccac tccattcaga ccctcgtcag atactcgttg gagcgcgtgt
      781 ccacccgcac cctatcgccc gcgttgataa agagcggcac ctgtacgacg atccctgtct
      841 ccagggttgc cggtttggac ccccctgtcg ccgtgtcgcc tttgaggcca gggtccgtct
      901 gagaaacccc gagttcgacg tgcgccggag gctctacgct gacgacctca gcgtcggccg
      961 atagtacggt gacggtgctg ccctcgacga tgtaccctga cgcctcccca acgacatcga
     1021 acggcactgg cacctgatcg taggtgtccg aatccatgaa atacagcagg tctccatctc
     1081 tgtagaggta tgtcatcgga cgcgactcgg tcctcacgtt ttcgagcttc tccccggccc
     1141 ggaaggtctt ctcgagcacg gccccactgt caatgtttct gagccgcgtc ctcacgaacg
     1201 ccccgccttt gccaggcttg acgtgctgga aattcaggat cgtgaagcgc ttgccgtcga
     1261 ccctgatcgc ggtgccgttc ctgaactggt tggtggatat catgttagga agctcccctt
     1321 tacggcgcct gatcttcgca gcaatcctgc gcggcgtaaa cccggcgcag acgccgcgct
     1381 attatcgcac agtttctttg cctgtcagcc cggcaccacg cagggattgc aacggttttt
     1441 cctgccggca ggcggcctct gatcagggac tgtcagaccg cccttcgagg acgtagcgga
     1501 cagcggcgac ataaccgtgg gcgcccatac ccgcgatcaa ggcgtccacc accggtgaga
     1561 tgaccgacac ccgacgccac tcctctctcg tgtgaatatt ggagagatga acctccacct
     1621 tcggtacatc caggacttcc agagcatcgt gcagagcgta agagtagtgg gtccacgcac
     1681 cagcgtttat gatgagcccg tcagagcccg ccgcttccct cgtaaacccg accatctcgc
     1741 cttctgaatc cgtctgcctg aactccaggt ccaatgccgg aaactcgtcc ttgatgagcc
     1801 tctctatgtc gtcgagggtc atggttccgt agatgtcggg gcggcgggtg ccgagggtgc
     1861 cgaggttcac gccgttgagg acgaagatct tactcaacga tcgttccgat cgcctcgcgg
     1921 acttcttctt cgctcacggg cacgctcctt acgggtctgc cgatgtcctc gaggagcacg
     1981 aacctgtgcc cggcgggtcc gtctgcagaa aagcgcttct tgtcgcggcc cattgcgaga
     2041 agcgcctgct cgaggccgac atcgggcacg cggcgcagca aaccggccgc cgatataagg
     2101 tcttcgtgta ctcgggtcag gtcccttccg aaccgggtgc gggagaggct tgcggcggcg
     2161 agcattccgg ccgcgacggc ctggccgtgc tgcaggttgt agccagcggc ggcctccagg
     2221 gcgtggccta cagtatggcc gtagttcaat atcgcccgca gcccgccttc cagctcgtcc
     2281 tcggctacaa cctgcgactt aaaccgtact gagtgtagga tgagcgtctc cagcccgtcg
     2341 aggacgcccg tgcgggcggc cccgatcaag ccgaggtcgt cgaagaagtc acctcccgag
     2401 aggaggccca tctttatggt ctcggcgagg ccgttggaga tctcacgctc tggcagcgtg
     2461 tcgacccagt ccaggtccgc cgccaccagg cggggcctga cgaaggcgcc gaccaggttc
     2521 ttgccttcgg ggaggtctac cccgaccttg ccaccaacag cgctgtccac catcgcgaga
     2581 agagatgttg gcagcatgac gaggtctacg ccccgcaagt aggtccccgc gacgaagccg
     2641 cccaggtccc cgaccacccc gccaccgagg gcgaagatcg taccgtcgcg ggtcagcctc
     2701 aggcgggcca ggcgcctgag ggcgtcggcg taggtctcta tgctcttgga ggcttcgccg
     2761 gagggaacct cgatcttccc tgcgacacgc cagcctgctc tctctagggc gccttgcacc
     2821 cgctccgcat gcagcggtcc taccgtcgag tcggtgaaga ttacgcagag accaggttcg
     2881 agcgaactcg agaagatcgc cgcgaggtcg atgccggcgc ctatgaagac cctatacggc
     2941 tgcgtgacgg ggaccgctac
//
LOCUS       DATMJA010000099         2096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_664821, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000099 DATMJA010000000
VERSION     DATMJA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664821"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..756)
                     /locus_tag="VNT12_02230"
     CDS             complement(145..756)
                     /locus_tag="VNT12_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI44961.1"
                     /translation="MEYTSTVTIGGWRERLGSLAVRREELNDKELVARTLSGDLRSYE
                     ELVRRYERLVGRVVYPYARREISVEDLVQETFLRAYDRLETFNPDYRFKTWLLAIANN
                     LGVDTLRRRREIVEFNPEAHAPVSRSPEAEAVEADRSRSVREAIATLPETYGVPLILR
                     YTEDLSYAEIAEVLSITVPAVKSRLFRARNMLAGRLEEAGERE"
     gene            complement(927..1547)
                     /locus_tag="VNT12_02235"
     CDS             complement(927..1547)
                     /locus_tag="VNT12_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44962.1"
                     /translation="MEEISRITKRRRRLRTVGEPHPWEPRHMRLTAMRFAESVGEPAE
                     RRNVTLGVIQHLWRSPVPFIGFYPPEGVKVTENRLYAPAPPRSVDDTDYLAGVEAHKR
                     AFAKDLQNAMIPAGRLPGYFEVPTDEGWRITLELAEGRLGLADAWGLNSERGVAGDLA
                     PFAWAYRNCDFCIFTIYDKDIGALRDDLLFVAKRAGVELRWRRNAA"
     gene            complement(1650..>2096)
                     /locus_tag="VNT12_02240"
     CDS             complement(1650..>2096)
                     /locus_tag="VNT12_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HWI44963.1"
                     /translation="EDNFRGNSIAGVSVAAHEVGHAIQDASGYVPMKLRAGIFPIVNF
                     SSQMWPLAFIMAFFVGGAASPFGSLLINVAILLFLGVLLFHVVTLPVEINASTRAYGL
                     LTRYGILSGTEVGGTRRVLTAAAFTYIAAALTAVLTFLYLLAVSRQ"
BASE COUNT          388 a          683 c          647 g          378 t
ORIGIN      
        1 cattgagcag cagctccttc tcgtacaggg ttcggcagcc actgcaatct ctcaagtgcg
       61 ccctgatctc ccgctcccgc tccggcccca gcgagccgtc ggccagctcg aagacgggct
      121 cggggtcgca gggaatcgga ccgttcactc ccgctccccc gcctcttcca gtcttccggc
      181 gagcatgttg cgggcgcgga aaagcctgct cttcaccgcg gggaccgtga tggagagcac
      241 ctcggctatc tccgcgtagc taaggtcttc ggtgtagcgg aggatcagcg gcacgccgta
      301 ggtctcgggc agggttgcga tcgcctccct gacgctcctg gaacggtcgg cctcgacggc
      361 ctcggcttca gggctccgcg agacaggggc gtgggcctcg gggttgaact cgacgatctc
      421 ccttcggcgc ctcagtgtgt ccacaccaag gttgttggcg atcgcgagta gccacgtctt
      481 gaagcggtag tcagggttga aagtctccag gcggtcgtac gcgcgcaaaa acgtctcctg
      541 aacaaggtcc tcgacggaga tctcacgccg agcgtaggga tatacgacgc gtccgaccaa
      601 ccgctcgtaa cgtcttacca gctcctcgta ggaacgcagg tctccagaga gcgtgcgtgc
      661 aacgagctct ttgtcgttca gctcctcgcg ccggaccgcc aggctcccca gcctctcccg
      721 ccagccacct atagtcaccg tggatgtgta ctccatacta agacgagtat acgctttcgg
      781 cccaggaaag tttccggcgg agcgcccgcc gcggtccacg taagcggtgg agtctctggg
      841 aaaaaggagc tgtctggaaa agcggcgaca gggaggttag atcgcggctt gattggcgag
      901 ggtctggccg cgtcgtcgag aatccctcag gctgcattcc tgcgccacct gagctccacg
      961 cccgcacgct tggcgacaaa gagaaggtcg tcgcgcaggg ccccgatgtc cttgtcatag
     1021 atggtgaaga tacagaaatc gcagttacgg tatgcccagg cgaagggcgc gagatccccg
     1081 gccacccccc tctcggaatt caggccccag gcgtcagcca accccaggcg tccctccgca
     1141 agctcgaggg ttatgcgcca cccctcgtcc gtcggaacct cgaagtagcc tggcaaccta
     1201 cctgcaggaa tcatggcatt ctgcaagtcc ttcgcgaagg ccctcttgtg ggcctcgacc
     1261 ccggcgaggt agtccgtgtc gtcaaccgac cttgggggag ccggcgcgta gaggcgattc
     1321 tccgttacct ttactccctc aggcgggtag aacccgatga aaggaaccgg tgagcgccag
     1381 aggtgctgta tcaccccgag cgtcacgttg cggcgctccg caggctctcc gacggactcc
     1441 gcgaagcgca ttgccgtcag gcgcatgtgc cttggctccc acggatgtgg ctctcccacg
     1501 gtccgtagcc gcctccggcg cttggttatg cgactaatct cttccatgac gcgcgtattc
     1561 tacaactctc gtgcggccga tgcccggcaa ggaaaagttc gagggcgggg ccgaagcccc
     1621 gccctcgtgg tcaagaggaa gagtctgcgc tactgcctgc ttacggccag caggtagagg
     1681 aaggtcagca ccgccgtaag agcagcagcg atgtacgtga aagccgccgc ggtcaggacc
     1741 cgcctggtgc ctcctacctc tgtgccagac agtatcccgt agcgtgtcag cagtccgtag
     1801 gcgcgggtgg aagcgttgat ctcgactggc agcgtcacca cgtggaacag aaggaccccg
     1861 aggaatagca agatggcgac gttgatcagg agcgacccga acgggctcgc cgcgccaccg
     1921 acaaagaagg ccatgatgaa agccagaggc cacatctggc tgctgaagtt cacgatcggg
     1981 aagatcccgg ccctcagctt catcggcacg tagccgctcg cgtcctggat cgcgtgcccc
     2041 acctcgtggg cggccacgct gaccccggct atggagttgc ccctgaagtt gtcctc
//
LOCUS       DATMJA010000100         9712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_191430, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000100 DATMJA010000000
VERSION     DATMJA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191430"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VNT12_02245"
     CDS             <1..462
                     /locus_tag="VNT12_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44964.1"
                     /translation="VQETSGAKGPIDHSSFQKTGTSVPEGGSREFVWTEDAIERLERA
                     PKGFMRNISRNMTEKLACERGVTHIDLALVEDSLTGARTTMEDVITGKVSIADLAKDT
                     GEKIEAEDGEAPHPPMTVTMVCTTCGEELQGVQPPEECPVCGAPGEDFEQR"
     gene            617..781
                     /locus_tag="VNT12_02250"
     CDS             617..781
                     /locus_tag="VNT12_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44965.1"
                     /translation="MDRGEEGCARVADGNRMERSRMEHWSYGARGRTEDDYHRHGLES
                     LGVTPGDASC"
     gene            775..1467
                     /locus_tag="VNT12_02255"
     CDS             775..1467
                     /locus_tag="VNT12_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI44966.1"
                     /translation="MLTRIVAALGVAVWCIFILLVSVVFVIDRGLADVGVVWVAVLGI
                     GVVAPVLLMRQISRAISAHEESRRSVPGTREKEKELLGALEERGRLTPTTAAMRTSLT
                     VDEASKMLDTLARDGHLELRTEEGVMAYALSEPGRQGLGKGSAYSPGAESGETPGRFV
                     AQERLEDPLSERELEVLSILASGKTNAEVAGDLFVSVGTVKSHTGNIYRKLGARNRAE
                     ALNRAREFGLIG"
     gene            1671..2126
                     /locus_tag="VNT12_02260"
     CDS             1671..2126
                     /locus_tag="VNT12_02260"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HWI44967.1"
                     /translation="MTRVVETPGGLARIETIVTPPKEGTKIIGLSVPVRLARIGYTVL
                     SLVFVACVLVQVFFAGMGAFGADWSLHLTFAHFLGLPPLLMAPMAFAGRLSWGLRLLP
                     LGLLVLIGVQYAFANAAVPAAALHPVNALLILCLSLLTARRAWAAVAKR"
     gene            2134..3696
                     /locus_tag="VNT12_02265"
     CDS             2134..3696
                     /locus_tag="VNT12_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HWI44968.1"
                     /translation="MKISRREFLRLGLAGGVTLAFGGSACSGSGEGSTGTVLPSKAKL
                     PEPFKVPLPVPPVLEPVRTDAGADYYEITQKAGRVEILPGLKTEVWGYDGIFPGPTIE
                     SRSGRKIIVRQRNELPVPVSTHLHGGRTPPESDGYPTDLIPPKNMTHDHSTTGHGSMG
                     TRGSRHSKDYVYPLEQRAATLWYHDHRMDFTAPQVWRGLAGFHIIRDDEDGALPLPKG
                     ERDVPLMICDRSFDEDGSFLYPSLDPSLQGKPGVEDDYMDGVLGDTILVNGAPWPVLE
                     VSATKYRFRILNASNARRYELALEPANHAPFAQVGSDGGLLGAPIGHRTIRIAQAERF
                     DVVVDFSKYNVGDEITLKNRTGQGKTASVMRFRVTRKENDDSTVPRRLSDMTEFDGLN
                     KSSAGRTREFRFTRVADGGRTMWGINGKPFDPERMAARPELGSTEVWHLRAAPAAHPI
                     HLHLVRFKVLSHNGRKSLPTDAGWKDTVDLASGEEARVLVRFDGYRGRYVFHCHNLEH
                     EDMMMMANFEVV"
     gene            3759..4082
                     /locus_tag="VNT12_02270"
     CDS             3759..4082
                     /locus_tag="VNT12_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWI44969.1"
                     /translation="MLEQARIRDLLSEYAGADMARSHKVTFQKSGITIDVAEDEYILE
                     EAEDAGLHLPYDCRSGTCTTCIQKCLEGEIDQDMAFAIGDEELEQGLRLICIGSPLSD
                     VVLDA"
     gene            complement(4131..5999)
                     /locus_tag="VNT12_02275"
     CDS             complement(4131..5999)
                     /locus_tag="VNT12_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44970.1"
                     /translation="MANEENLDARYAETLLRRFEPVIRFTKGEWFYPMDAEPYVDACS
                     LWVRHPGEDAVCVVPAGALTLERLAQQPEDSTGAVHYLKIPTSKQDERRGLWARLRPR
                     RSHKDPDWPRETFRAGRGRLARVGYISRLADALFSLVLLARGRVPGGDVEGARVLYGR
                     LMEKEEHFRYHGRVIRQGGWIVLQYWLFYAFNDWRSNFFGANDHESDWEKVMVFLSES
                     ESGEVRPEWVAYAAHEETGDDLRRRWDDPLLEKSGEHPVVYVCAGSHASRYSRGEYLT
                     ELAFPLPRAFAAARRFARRFWYITLRQYTDEASAELPYERFTIPFVDYGRGDGLSVGP
                     GQEREWDPPRLMQDPLPSWVAGYRGLWGLFTRDPFEGEDAPAGPMYDRDKTISREWYD
                     PVGWAGLDKVPTRTEELDVILKRQAEIEQRRNLLRSEILEKSSRLKALGMDAEATRDR
                     SHLTKLYETQMRNVEALSKEVEEMRAELAEEEVLHESLGEHAERLRNGERSPAHAHMA
                     HPVRPVSEEETRAGRVAQLWASISVAVALVTLIVIFAYQRQHLLSALVFAIAMFAFIE
                     AGFRRRLTRFIVSANIGLAIVGALVLLQHYFWQIIVLAVLGVGLYILWENLRELGR"
     gene            complement(5989..6450)
                     /locus_tag="VNT12_02280"
     CDS             complement(5989..6450)
                     /locus_tag="VNT12_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44971.1"
                     /translation="MYRQTRILGGLLILQVVAMVGFGVWLFSTIDWDRILTLANEQAQ
                     NLPKPLERQLEQAILYAIYFLPPAILLLLAGLGFVLLRRRGWMLASVAQAIIMLVCLF
                     FYTDPRPWFAYPIIAYCILMILYLNSQGVRAVVHSRRMAPAGARSETVRGE"
     gene            complement(6560..7699)
                     /locus_tag="VNT12_02285"
     CDS             complement(6560..7699)
                     /locus_tag="VNT12_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI44972.1"
                     /translation="MQTIDLTQVPVVDNHCHGIMQDQTFDDLAIWRHSFTESTDPGMA
                     RDHVASTAFYRRLIRTLADFLGCEPEEEAVFAARSGKNGRELTGALLRAANVETFLLD
                     TGFPPPEEVFPIPELGEIGGCRTEPMLRLEILMESLLAEHYSLEDVKEALAAELDDVR
                     GRGYVALKSIAAYRTGLDIREWPREEAEELFHGYRRVAGAGHARLVHKPLLDTLLHVA
                     FAQSARQEVPVQFHVGYGDADTDLLLGNPLYLRPVFQRSDYHGMPVVLLHECYPYTRQ
                     GGYLAAVYENVYLDLSYGIPLLGYGEMLSFTRQALGVAPSSKLMYSSDGIGVPELHWI
                     SAIDGRRAIGEALGELVSRGELDLSDAEAAGESILRANATRLYRL"
     gene            complement(7801..9381)
                     /locus_tag="VNT12_02290"
     CDS             complement(7801..9381)
                     /locus_tag="VNT12_02290"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWI44973.1"
                     /translation="METVIQGSTLQTLRAGLRGTAHAPGEEGYEEASRAWNLADLQTA
                     MIHTTSSASTATSSPLRRGRTPSVEGRGIQRTLEGEMQAEREVQENVARPLPEDTLWV
                     RFVFVDHAGIPKAKAVHRDGFEGRARAGVGLAKGVLALDPSGALHPESGLSPVGECRL
                     VPDLSTLTALPFARGQAMVSVDMTEPDASTPWEGCPRSALKRVLERLSDRGYRTLASY
                     EAEFYVWDAEGPLDRTPYAGSYALTAAADFVAELAQTLEEMGIRPEQCHAEVGQGNLE
                     LSVGEDEALAAADKRVMVLEAIRGVAYRMGLGTTMAPKPYLEGAGNGHHLHLSLHRDE
                     TPVLFDATGALSGPGSGFVAGILQHMPAVMAFTAPSPNSYQRLAPGMWASAYAAFGLD
                     NREAAVRLASPVAGRESATANVELKPVDVTSNPYLALAAVLAAGLDGIERGLDPGEPT
                     MVDPATLSDEERASRGILPLPASPDEALDALEEDHVLMEALGEPLVRTHVAVARAQAN
                     MARELPPEEVAATAAMLY"
     gene            complement(9511..>9712)
                     /locus_tag="VNT12_02295"
     CDS             complement(9511..>9712)
                     /locus_tag="VNT12_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HWI44974.1"
                     /translation="AQNEQVKKISAWAAILFAPTLIGTIYGMNFDYMPELGWLLGYPF
                     ALVLMAFTSLTLYLIFKRRGWM"
BASE COUNT         1829 a         3090 c         3043 g         1750 t
ORIGIN      
        1 gtgcaggaga cctcgggcgc gaagggcccc atcgaccact cctcattcca gaagaccggc
       61 accagcgtcc cagagggcgg ctccagagag tttgtctgga ccgaggacgc catagagcgc
      121 ctggagcggg cgcccaaggg ctttatgcgg aacatctccc gcaacatgac cgagaagctg
      181 gcgtgcgagc gcggcgtgac gcacatagac ctcgccctcg tcgaggactc gctcacaggg
      241 gcacgcacca cgatggagga cgtcataaca ggcaaggtct ctatcgcaga cctcgcaaag
      301 gacacgggcg agaaaataga agccgaagac ggtgaggctc cgcacccgcc aatgaccgtc
      361 acaatggtct gcactacctg cggcgaggag ttgcagggcg tccagcctcc agaggagtgt
      421 cccgtctgcg gcgctccagg agaggacttc gagcagaggt aggctacagg tgtcaggctt
      481 caggaaacct tagagcctgg aagccaggaa aagccccggt cccacgagcc gggaccgggg
      541 ctttcgttcg cgggtcttga atcttcgggt cgtatctttg cgtatactct ttaggatgtc
      601 gcgggcgtag gatgcagtgg acagaggcga ggaaggctgc gcccgggtcg cggacggaaa
      661 caggatggag agatcccgga tggagcattg gagctacggg gcgcgcggac ggaccgaaga
      721 cgactaccac aggcacggtc tggagagcct cggtgtcact cccggggatg cgtcgtgctg
      781 acccggattg tggcggcgct tggcgttgcg gtgtggtgca tcttcatcct gctcgtttcg
      841 gtagtgttcg tgatagacag gggactggcc gatgtgggcg tagtctgggt agccgtcctt
      901 ggtatcgggg tggttgctcc cgtactactc atgaggcaga tcagccgcgc tatatcggcg
      961 cacgaagagt cccgccgttc cgtcccaggg acgagggaga aggagaaaga gctccttggc
     1021 gccctggagg agcgcggcag gctcacccct accacagccg ccatgcgaac ctcgctcacc
     1081 gttgacgagg cgtcgaagat gctcgatacg ctggcccgcg atggccatct ggaactgcgc
     1141 acggaggaag gcgtcatggc ctacgccctg agcgagccag gcaggcaagg gttgggcaag
     1201 ggctccgcgt actcgccggg agcagagagc ggcgaaacac caggccggtt cgtcgcgcaa
     1261 gagcgactcg aagatccttt gagcgagcgc gagctcgagg tgctgagcat actcgcctcg
     1321 ggcaagacca acgcagaggt ggcaggggat ctctttgtct ccgtgggcac cgtcaagagc
     1381 cacaccggca acatctaccg caagctcggc gcaaggaacc gcgccgaggc cctcaacagg
     1441 gcgcgcgagt tcgggctgat tgggtaggct tctcttcggg cttgtaagcg ctcccgccaa
     1501 catgcgggtg ccctccgatc caccgcagga acaagcctcc ccctcctgca acgggccacc
     1561 aatgaacccc gatgaaccac ccagatctgc ctttcggccg atgcctctgc gaccggttca
     1621 gccgtacttt cagggtgaga cgtaagcaac ctgcaaggag gcaccctgca atgacgcgtg
     1681 ttgtggagac gccgggtggt ctggcgcgaa tcgagacgat cgtgacgcca ccgaaagaag
     1741 gaacgaagat catcgggcta tccgtccccg ttcggctggc ccgaatcggc tacaccgtgc
     1801 tctccttggt cttcgtcgcc tgcgtacttg tacaggtgtt cttcgccgga atgggagcgt
     1861 tcggggcgga ctggtccttg cacctgacct tcgcgcactt ccttgggctg ccgcctctgt
     1921 tgatggcacc catggctttc gccggccggt tgtcgtgggg cctcaggcta ctgcctctcg
     1981 ggctcctggt cttgatcggc gtgcagtacg ccttcgccaa cgccgctgta ccggcggcgg
     2041 ccctgcaccc ggtgaacgcg ttgcttatcc tctgtctgag cctgctcact gccaggcggg
     2101 cgtgggctgc cgtggccaag cggtgatcct ttcgtgaaga tctcacgcag ggagttcttg
     2161 aggctggggc tcgcgggcgg ggtgacgctc gctttcggcg ggtccgcgtg ctctggttct
     2221 ggcgagggct cgacggggac ggtgttgcca agcaaggcga aattgccaga gcctttcaag
     2281 gtaccgctgc cggtgcctcc ggtgctcgag cccgtgcgca ccgacgcggg cgccgactac
     2341 tacgagatta cccagaaggc aggcagggtg gagatcctac caggcctcaa gacggaggtc
     2401 tggggctacg atgggatctt ccccggccct accatagagt ctcgcagcgg caggaagatc
     2461 atagttcgcc agcggaacga gttgcccgtg cctgtctcca cgcacctgca cggcggcagg
     2521 acgcctcctg agagcgacgg ctaccctacc gacttgattc cgccgaagaa catgactcac
     2581 gaccattcga caaccgggca cggctcgatg ggcacgagag gttctcggca ctccaaggac
     2641 tacgtctacc cgctggaaca gcgcgccgcg accctctggt accacgacca ccgaatggac
     2701 ttcaccgctc cgcaggtctg gcggggactg gccggcttcc acatcatccg cgacgacgaa
     2761 gatggtgccc tgcctcttcc caagggcgaa agggatgtcc cgctcatgat ctgcgaccgc
     2821 tccttcgatg aggacggctc gttcctttat ccttcactgg acccctcatt acagggcaaa
     2881 cctggcgtcg aggacgacta catggacggg gtccttggag acaccatcct ggtcaacggc
     2941 gctccctggc cagtactgga ggtgtcggcc acgaagtacc ggtttcgcat cctcaacgcc
     3001 tccaacgccc gccgctacga actcgccctg gagccggcaa accatgcccc tttcgcccag
     3061 gtcggcagcg acggggggct gctcggcgcg ccaatcggac accgcacgat ccgtatcgca
     3121 caagcggagc gcttcgacgt ggtcgtagac ttctcaaagt acaatgttgg cgatgagatc
     3181 acactaaaaa accgcacggg ccaaggcaag accgccagcg tgatgcgctt ccgcgtaacc
     3241 cgcaaagaga acgacgacag cacggtaccg cgacgacttt cggacatgac ggagttcgat
     3301 gggctcaaca agtcaagcgc cgggcgcacg cgcgagtttc gctttaccag ggttgcagac
     3361 ggcggcagga cgatgtgggg tatcaatggc aaaccgttcg acccggagcg catggccgcc
     3421 aggccggagt taggatccac cgaggtctgg cacctgaggg cggcccccgc ggcgcacccg
     3481 atccaccttc acctggtccg cttcaaggtg ctctcccaca acggcaggaa atctttgcca
     3541 acggacgccg gctggaagga caccgtggac ctggcctcgg gcgaagaggc gcgtgtgctc
     3601 gtccgtttcg acggctaccg gggccgctac gtcttccact gccacaacct cgaacacgag
     3661 gacatgatga tgatggccaa cttcgaggtc gtctgagagt tccgccggcc gcaccggatg
     3721 caagcggtca aatcgttcgc taaaaaggac ctgccagtat gctagaacag gccaggatac
     3781 gagacttgct ctcagagtac gcaggagcgg acatggccag atcacacaaa gtaaccttcc
     3841 aaaagagcgg catcaccata gacgtggccg aagacgagta catactcgaa gaggccgagg
     3901 acgcaggcct ccacctcccc tacgactgcc gcagcggcac gtgcacgact tgcattcaga
     3961 agtgcctgga aggtgagatc gaccaggaca tggccttcgc cataggcgac gaggagctgg
     4021 agcaaggtct gcgcctcatc tgcatcggca gtcccttatc tgacgtggtg ctcgacgcat
     4081 gaaagaaatc caaaatacgc gagtccggac actcagtagg agaaaaacac ctaccgtcca
     4141 agctcccgca ggttctccca caggatgtag aggccgacgc ccaggaccgc taggacgatg
     4201 atctgccaga agtagtgttg gaggaggacc agggcgccta ctatcgccag tccgatattg
     4261 gcactaacta tgaacctggt gagtcgcctc ctgaacccgg cctcgatgaa cgcgaacatg
     4321 gcgatagcga agacaagcgc ggagagcagg tgctgtctct gataggcgaa gatgacgatg
     4381 agagttacga gcgctaccgc aacactgatc gaagcccaaa gctgcgccac cctaccggcg
     4441 cgggtctcct cctcggagac gggtcgcacg gggtgcgcca tgtgggcgtg ggcgggggaa
     4501 cgctcaccgt ttctcaggcg ttcggcatgt tcgcccagcg actcgtggag gacttcctct
     4561 tcggcgagct ccgcgcgcat ctcttctact tctttggaga gcgcttcgac gttccgcatc
     4621 tgggtctcgt agagcttggt caggtgggat ctgtcgcgtg tggcttcggc gtccatgccg
     4681 agcgccttta gtcggctgct cttctctaat atctcggagc gaaggagatt gcggcgctgt
     4741 tcgatctccg cctggcgctt gaggatgacg tccagttcct cggtccgggt tggcaccttg
     4801 tcgagcccgg cccagcccac ggggtcatac cactccctgc taatggtctt atcgcggtcg
     4861 tacatcggcc ccgcaggggc atcttcacct tcaaaaggat ctcgggtgaa gagcccccat
     4921 agcccccggt aacccgcgac ccaggaagga agcggatcct gcataagacg cggagggtcc
     4981 cactctcttt cctgacccgg tccgaccgac agtccgtccc cacgcccata gtccacgaag
     5041 ggtatggtga acctttcgta gggcaactcc gctgaggcct cgtccgtata ctggcgcagt
     5101 gttatgtacc agaacctccg cgcaaacctt ctggccgcgg cgaacgcccg cggcagtggg
     5161 aacgcgagtt cggtcagata ctcgccccga gagtaacggc ttgcgtgcga gccggcgcag
     5221 acgtatacaa caggatgctc gccgctcttt tcgagcaacg ggtcgtccca cctgcgtctg
     5281 agatcgtcgc cggtctcctc gtgtgcggcg taggctaccc actcagggcg cacctcaccg
     5341 gactcggatt ccgagaggaa gaccataacc ttctcccagt cggactcatg gtcgttagcg
     5401 ccgaaaaagt tgctcctcca atcgttgaac gcgtagaaga gccagtactg caagacgatc
     5461 cagccgcctt gccggatcac tcgcccgtgg tacctgaagt gttcctcttt ctccatcagg
     5521 cggccgtaca agacgcgcgc cccctctaca tctccgccag gaactctgcc cctggccagc
     5581 aagaccaaag agaagagcgc gtcagccaac cgcgagatgt aacccacgcg cgcgaggcgg
     5641 cccctgcctg cgcggaaagt ctcccgaggc caatctgggt ccttgtgtga gcgcctcggc
     5701 cgcaagcgcg cccagagacc acgcctctcg tcctgcttgg aagtgggaat cttgaggtag
     5761 tgtacggctc cggtactgtc ctccggctgc tgggcgagtc tctccagggt gagagccccg
     5821 gccggcacca cgcagacggc gtcctcacca ggatgtcgga cccacaggct gcaagcgtcg
     5881 acgtaaggct cggcatccat agggtagaac cactcgcctt tggtgaaccg gatcacgggc
     5941 tcgaagcggc ggagtagggt ctccgcgtac ctcgcgtcca ggttctcttc attcgccacg
     6001 cacggtctcc gatctagcgc ccgctggtgc catccgcctg gagtggacca cggcccgcac
     6061 accctgagag ttcaggtaca ggatcatcaa gatgcagtag gcgatgatag ggtaggcgaa
     6121 ccatggtcgc gggtcggtat agaagaacag gcagaccagc atgataatgg cctgcgcgac
     6181 cgaggccagc atccaccccc ggcgccgaag tagtacgaaa ccgaggccgg cgagaagtag
     6241 cagtatcgcg ggcgggagaa agtagatcgc gtacaggatc gcctgttcga gttgcctctc
     6301 cagcggcttt ggaaggtttt gcgcctgctc gttggcgaga gtcagtatcc ggtcccagtc
     6361 gatggtgcta aacaaccaga cgccgaatcc caccatcgcg acgacctgca agatgagcag
     6421 cccgccgaga attcgcgtct gtcgatacaa tgccagcctt ccgcgttcgg ttaccagcct
     6481 ggatcacgca ctggcaggta ggataaccgc ttgcgtccgg tcacgcacct tcctgcccga
     6541 ccctggctct cgaaactacc tagagcctgt acaggcgtgt cgcgttcgca cgaaggatgc
     6601 tctcaccggc ggcttcggcg tctgagaggt ctagctctcc tcgagacacg agctctccga
     6661 gtgcctcacc gatcgcgcgc ctgccgtcta tggcgctgat ccaatgtagc tcgggtacgc
     6721 ctatgccgtc ggaactgtac atgagcttgg aactcggagc gacgccgagc gcctgccgcg
     6781 tgaaggagag catctctccg tagccgagca ggggaatacc gtaggagagg tccaggtaga
     6841 cgttctcgta gacggcggcc aggtagccgc cttgcctggt gtacgggtag cactcgtgca
     6901 gcaacacgac gggcatgccg tgatagtcgg accgctggaa caccgggcgc aggtagagcg
     6961 ggttaccgag caggagatcg gtatctgcgt cgccatagcc gacgtgaaac tggaccggga
     7021 cctcctgccg ggccgactgc gcgaacgcaa cgtggagcaa ggtgtccagg agcggcttgt
     7081 ggacgaggcg cgcatggccc gctccggcga cccgcctgta accgtggaac aattcttcgg
     7141 cctcctcgcg gggccactcc cgaatgtcaa ggcccgtcct gtaggcggcg atgctcttga
     7201 gtgccacgta gcccctgccc cgcacgtcgt ccagttcggc ggcgagcgcc tctttcacgt
     7261 cctctagaga atagtgttcc gcgagcaaac tctccatcag gatctcgagc cgcagcatcg
     7321 gctccgtgcg gcaacctccg atctctccta gctctggtat aggaaacacc tcttcgggcg
     7381 gcgggaagcc cgtgtccagg aggaaggttt cgacgttcgc ggcgcgcaac aacgcgcctg
     7441 ttagctcccg tccgttcttc ccgctcctgg ccgcgaagac ggcttcctct tccggctcgc
     7501 agccgaggaa gtcggccagc gtccgaatga gacgccggta gaaagccgta ctcgcgacgt
     7561 ggtctcgcgc cataccaggg tccgtggact cggtgaagga atgtcgccag atcgcgaggt
     7621 catcgaacgt ctggtcctgc attatgccgt ggcagtggtt gtcgacgacg gggacctggg
     7681 tgaggtctat cgtttgcatg ggccgcttcc gttcaaagct tcccttcccc acctttcttg
     7741 caggtatcag gttacaggta ttttgctgat gcctgtagcc tgatgcctga aacctatctt
     7801 ctagtagagc atcgcggctg tggccgccac ctcctcaggg gggagctctc tggccatatt
     7861 ggcctgcgcc cgagccacgg ccacgtgggt gcgcacgagt ggttcgccca gcgcctccat
     7921 gaggacatga tcttcttcca gcgcgtccag ggcctcgtcc ggggatgcgg ggagtggcag
     7981 gatacccctg gaggcgcgct cctcgtcgct gagcgtggcc gggtccacca tcgtcggctc
     8041 cccggggtcg aggccgcgtt ctatgccgtc cagcccggcg gccaggaccg cggccagggc
     8101 caggtagggg ttcgacgtaa cgtcgaccgg cttgagctcg acgttggcgg tcgcggattc
     8161 cctgccagcc accggggagg cgagccgcac ggcggcttcc ctgttatcca ggccgaaggc
     8221 ggcgtaagcc gaagcccaca tgcctggagc aagccgctgg taggagttgg gcgacggtgc
     8281 ggtgaacgcc atgacggcgg gcatatgctg cagtatcccg gctacgaagc ccgagccagg
     8341 accgctcagg gctcctgtgg cgtcgaagag caccggagtc tcgtctctgt ggaggctgag
     8401 gtgcaggtgg tggccgttgc ctgctccttc taggtatggc ttcggggcca tcgtggtccc
     8461 gagccccatc cggtaggcca cgcccctgat cgcctccagc accataacgc gcttgtccgc
     8521 cgcagccaac gcttcgtcct cccccacgga gagctcgagg tttccctgac cgacctcggc
     8581 gtggcactgt tccggccgga tacccatctc ctccagcgtc tgtgccagct ccgcgacaaa
     8641 gtccgcagcc gcggtcagcg cgtagctccc cgcgtacggc gtcctgtcca gcggaccctc
     8701 ggcatcccag acgtagaact cggcctcgta ggaggcgagc gtcctgtagc ctctatccga
     8761 gagccgttcg agcacccgct tgagggcgct acgcgggcag ccctcccagg gcgtgctcgc
     8821 gtcgggctcc gtcatgtcga cggagaccat cgcctgcccg cgggcgaagg ggagggctgt
     8881 aagtgtcgag aggtcaggga ccagccggca ctctcctacc gggctcaagc ccgactccgg
     8941 gtgcaaagcc ccgctcgggt cgagcgccag aactcctttg gcgagcccaa ccccggccct
     9001 cgccctccct tcgaagccgt ccctgtggac ggccttcgcc ttggggatgc cagcgtggtc
     9061 cacgaacacg aaccggaccc acaacgtatc ctcaggcaac ggccgcgcca cgttctcctg
     9121 tacctcgcgc tcggcttgca tctcaccctc caacgttcgc tgaatacctc tcccttccac
     9181 cgagggggta cgcccccgcc ttagagggga cgatgttgcg gttgaagcgg aagacgttgt
     9241 gtggatcata gcggtctgaa ggtcggccag gttccacgcc cggctcgcct cctcatagcc
     9301 ttcctctccg ggcgcgtggg cggtgccgcg caggccggcc cgcaaggtct gtagcgtcga
     9361 accctggatc accgtctcca taatcttctc ccctctctca gggtttcgag ccggtagggt
     9421 aattcctccc gaccggtcca gacaacggac gcagcctaac ctgcaagtgt agaagatgca
     9481 tcccccaaat gggctatttt ggaagttggc ttacatccag ccgcggcgtt tgaagatgag
     9541 gtagagcgta agagaggtga aagccataag caccagggcg aaggggtagc cgagcagcca
     9601 gcccaactcg ggcatgtagt cgaagttcat cccgtagatc gtccctatta gtgtgggcgc
     9661 gaacagtatg gcggcccagg cggagatctt ctttacctgc tcgttctggg cc
//
LOCUS       DATMJA010000101         3967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_428380, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000101 DATMJA010000000
VERSION     DATMJA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428380"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VNT12_02300"
     CDS             <1..1254
                     /locus_tag="VNT12_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI44975.1"
                     /translation="AGAASLLLTRQVTRPLSETRDAAIRVASGDYAATVPVKSRDELG
                     EVARTFNYMAEEIEHYVGEIQEQKSRLEAVLEASPEAVVATDSGERVTMVNAAAARML
                     GIRASDRGRTLEEIGAPSAVLRCLREAAANGVAVREVELGEKAFWAYAAQMDRGQPGN
                     APSGDAGIILAVRDITEHRSLEKAKTAFVSDLSHELRTPLTTIQSAVGLLERARDRLD
                     PLEHRALELADQELKRIRGMVEELMTLAQMDSWKYQLEVGPANMSTVVQTAIESVETK
                     AQRFGIKIYFDDAGEHRCICDVQKLYQIFLNLMDNAIKYSDSGARVDVEIEEVDSTLT
                     VRIRDTGVGIPKEDLNQLFERFYRVDKDRSRATGGSGLGLAISRQIVEMHGGSISVES
                     EVDVGSVFEVRLPKAFLSRSVSHAL"
     gene            1244..2452
                     /locus_tag="VNT12_02305"
     CDS             1244..2452
                     /locus_tag="VNT12_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44976.1"
                     /translation="MLSRVGSAKRVAVLSVAAVFAAGCAHVDKEVSIAPLVPAAGRVD
                     PGLGLGTRALSSGPGYKGSPSWSPGDDRIAFTVDDYVEDRPTDSEGLRRSTTRDFIAE
                     DTEWISEDALMILGAASPSGTQETPSSLYRARADEDSLELEKVEKEVSAIGPGRGGLI
                     FAFGRGAFESGLALTRGSGDVRRLYSKSIKGRVASLSLSPDGDEAVLAVRPPGDLETS
                     VLHVFDLRKGEGREITRLDGNQEILGTPQWMGQYLYFVAGEKNMPVDRDGSEPHYEIY
                     RVPEDGGAPEPAPGVGEDFVAASIRVSPDSERLAVIGRLNPKSPTNLYVLNPLAKDLT
                     AVTMNEDMEIKTGPDDLAWSPGGESVAVIARGTPSTEPEVRAAPADRLLRDFYNLYEI
                     PVAGPEDAPR"
     gene            2449..2952
                     /locus_tag="VNT12_02310"
     CDS             2449..2952
                     /locus_tag="VNT12_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44977.1"
                     /translation="MRDIVFWLSGAALAFLVTAGAFLFLANLATAPSEKRLNPDQASG
                     ESGQAVDLILDRELLASLRSGPDQSLDLVVENEGTKQLSDVNVTLRVFSENTALSDSR
                     YYRQTVEKIPASEAARVRFEFDLPAREDPVARPPTANPEPSREILEIRATTPDGNSAV
                     RTVILPL"
     gene            complement(2957..3571)
                     /locus_tag="VNT12_02315"
     CDS             complement(2957..3571)
                     /locus_tag="VNT12_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44978.1"
                     /translation="MKPTSFVPMVGEISGEDACEVPGLGAVISYWTGDRYSAEEWFRE
                     LNDFWGTAGFAVRRGGEVQGFVVYGPPERLARAARYPLGPIDDEAVLLAYAGGDVRTR
                     RRLLVRMLRDLRQRGVGRVEAIASDRGVSHHVPTSFLLESGWQPVRRAPYRMSYYTLA
                     HIDLKSAVEVGELARALVGRVKLPGLKSPVPGALARSAGEPRSR"
     gene            complement(3568..>3967)
                     /locus_tag="VNT12_02320"
     CDS             complement(3568..>3967)
                     /locus_tag="VNT12_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWI44979.1"
                     /translation="TFVLGGAVGALVGILLAPRSGKELRGSLANRAGEARERGRETYF
                     DAQEQLRERLAEVREGSRQRPEVDDLGDSSPASGKAEVRRPYLRDVSRDVEPEAVEEI
                     ADAERSEQLRRKVRETRERLRGPKDSGPER"
BASE COUNT          766 a         1284 c         1275 g          642 t
ORIGIN      
        1 gccggggcgg cgagcctgct tctcacgcgt caggtcacgc gtcccctctc cgagaccagg
       61 gatgcagcca taagagtcgc ctccggggat tacgcggcga ccgtgcccgt caagagcagg
      121 gacgagctcg gcgaggtagc aaggactttc aactacatgg ccgaggagat agagcactac
      181 gtcggggaga tccaggagca aaagagccgt ctggaagccg tcctcgaagc ctctcccgag
      241 gctgtcgtgg caacggactc cggcgagcgg gtcacgatgg tcaacgcggc ggcggccagg
      301 atgctgggca taagggcctc agacaggggg cgtacgctgg aggagatcgg tgcccccagc
      361 gcagtcctca ggtgcctgcg cgaagcggcc gccaacgggg ttgcggtaag ggaggtcgag
      421 cttggagaga aggctttctg ggcttacgcc gcccagatgg acagggggca gcccggcaac
      481 gcgcccagcg gcgacgccgg catcatcctg gccgtaagag acataaccga gcaccgctcg
      541 ctggagaagg ccaagactgc cttcgtctcg gatctttccc acgagctcag gaccccactg
      601 accaccatcc agagcgcggt cggcctgctg gagcgggcac gcgaccggct cgacccgttg
      661 gagcaccggg cccttgagct ggccgatcag gaattgaagc gcattagggg gatggtcgag
      721 gagctgatga ccctggccca gatggactcc tggaagtacc agctcgaggt agggccggca
      781 aatatgagta cggtcgtcca gacggcaata gagtcggtag agaccaaggc ccagaggttc
      841 ggcatcaaga tatacttcga tgacgcgggc gagcacaggt gcatctgcga cgtccagaag
      901 ctctaccaga tcttcctcaa cctgatggac aacgcgatca agtactccga ctcaggcgcc
      961 agggtggatg tagagataga ggaggtcgac tcgacgctga ccgtccgcat ccgtgatacg
     1021 ggcgtgggca tccccaaaga ggatctgaac cagctcttcg aacgtttcta cagggtggac
     1081 aaggaccgct cccgcgcgac cgggggaagc ggacttgggc tcgctataag caggcagata
     1141 gtggagatgc acggcggcag catctccgtc gagagcgagg tcgacgtcgg ctccgtcttc
     1201 gaggtcaggc tcccgaaggc atttctctcc cgctcagtaa gtcatgctct ctagagtagg
     1261 gtcggcaaaa cgggtcgcag tcctctcggt agcggctgtc tttgcggcgg ggtgcgccca
     1321 cgtcgataaa gaggtctcta tcgcgcccct cgtcccagca gcgggcaggg ttgaccccgg
     1381 actcggcctg gggacacgag ccctcagttc gggacccggg tacaaaggct cgccgagctg
     1441 gagccctggg gacgacagga tcgcatttac ggtagacgac tacgtggagg acaggccgac
     1501 tgactcggaa ggcctgaggc gctcgaccac cagggatttc atcgcagaag atacagagtg
     1561 gatctcggaa gatgccctga tgatactcgg cgcggcgtcg ccctcaggga cacaggagac
     1621 cccgagctcc ctctataggg cgcgagcaga tgaagattcg ctggaactag agaaggtcga
     1681 gaaggaggtt tcggccatcg gcccaggcag gggaggtttg atcttcgcct tcggtcgcgg
     1741 ggctttcgag agcgggctcg cgttgacgcg aggcagtggg gacgtccgca ggctctactc
     1801 caaatctatc aaaggccgcg tcgcgtccct ctccctctcg ccggacggcg acgaggccgt
     1861 cctggctgtg cgtcctcccg gagacctcga gacctcggtg ttgcacgtat tcgatcttcg
     1921 gaagggggaa gggcgagaga taacgcggct ggacggcaac caggagatcc tcggcacgcc
     1981 ccagtggatg gggcagtacc tctacttcgt ggccggggag aaaaatatgc cagtagacag
     2041 ggacggctca gagccacact acgagatcta tcgtgtgcct gaggatggcg gcgcgccgga
     2101 acccgcgcct ggtgtgggag aggactttgt agcagcgagc atccgagtct ctcccgacag
     2161 cgagcgattg gccgtcatcg gacgcctcaa cccgaagtcg ccgaccaacc tgtacgtact
     2221 gaaccccctc gcgaaggacc tgacagctgt aacaatgaac gaggacatgg agataaagac
     2281 cgggccggac gacctcgcgt ggtcgcccgg cggcgagagc gtcgcggtga tcgcccgcgg
     2341 caccccatct acggaacccg aggtgcgtgc ggcccccgca gataggctcc tgagagactt
     2401 ctacaacctc tacgagatac ctgtcgctgg ccccgaggat gccccgcgat gagggacata
     2461 gtattctggc tctccggcgc ggccctggcc ttcctggtca cggcgggagc tttcctgttc
     2521 ctggcgaacc tcgccaccgc cccttccgag aagcgtctga atccggacca ggcaagtggc
     2581 gagtccggac aagccgtgga cctgatcctg gacagagaac tcctcgcgtc tctcaggtcc
     2641 ggccccgatc agagtctcga cctggtagta gagaacgagg ggactaagca actctcggat
     2701 gtgaacgtga ccctgcgcgt cttctccgag aacacagcac tctcggactc ccgctactac
     2761 aggcagactg tcgagaagat accggccagc gaggcagcca gggtgcgttt cgagttcgac
     2821 cttccggccc gcgaagatcc tgtcgcgaga ccacccacag cgaaccctga accatcgcgt
     2881 gagatcctgg agataagggc caccacgccc gacggcaact cggcggtcag gacagtgatt
     2941 ctgccactat gaggccctac cggcttcttg gctcgcccgc actgcgggcc aacgcgccgg
     3001 gcacaggcga cttgagcccc ggcagtttta cccgcccgac cagcgcccgc gccagctccc
     3061 ccacctcgac ggcgctcttc aggtcgatgt gcgcgagcgt gtagtagctc atacggtaag
     3121 gggcgcggcg cacgggctgc cagccactct caagcaggaa cgaagtaggc acgtggtggg
     3181 acaccccacg gtcgctggct atcgcctcga ccctcccgac gccgcgctgc ctgagatccc
     3241 tgagcatcct caccaacagg cggcggcgcg tgcgtacgtc tcccccggcg taagccagca
     3301 gcacggcctc atcatcgatc ggccccagag gataacgcgc cgcgcgcgcc aggcgttcgg
     3361 gcggcccgta gaccacgaaa ccctgaacct ctccaccgcg ccgcacggcg aaacccgccg
     3421 tcccccagaa gtcattcagc tcgcggaacc actcttcggc ggagtaccta tcgccagtcc
     3481 agtacgagat cacggcccca agaccgggca cctcgcaggc atcctcgcct gagatctcgc
     3541 cgaccatcgg cacgaaagac gtgggtttca tcgctccgga ccactgtctt tcggtcctcg
     3601 cagccgttcc ctggtctccc tcaccttccg ccgtagctgc tcggacctct cggcgtcggc
     3661 aatttcctcg acggcctcgg gctcgacgtc gcgtgatacg tccctgaggt agggtcgccg
     3721 cacctccgcc ttccctgagg cgggggagga gtcgccgagg tcgtcgacct cggggcgttg
     3781 gcgtgaccct tcgcggacct cggccagacg ctccctcaac tgctcttgcg catcgaaata
     3841 ggtctcgcgg cccctctcgc gggcctcacc ggccctgttg gcgagcgaac ccctgagctc
     3901 tttgccgctg cggggggcca ggagtatgcc caccagggcc cctaccgcgc cgccgagtac
     3961 gaaggtt
//
LOCUS       DATMJA010000102         2435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_488098, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000102 DATMJA010000000
VERSION     DATMJA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488098"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VNT12_02325"
     CDS             complement(<1..363)
                     /locus_tag="VNT12_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWI44980.1"
                     /translation="MVKRVCVFDVNETLLDLAALDPLFERVFGDTFVRRLWFNQMLQS
                     AFVATITDAYTTFGEAAGAALRMTEERLGVEVSEDDRQEILGGLRDLPPHPEVPDSLD
                     RLRDAGFRLATLTNSTQEV"
     gene            complement(487..1410)
                     /locus_tag="VNT12_02330"
     CDS             complement(487..1410)
                     /locus_tag="VNT12_02330"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HWI44981.1"
                     /translation="MEKFIISAAITGGMTVPGQSEAIPITPDEIVDSAVGAHEAGATI
                     VHLHVREPETGNPSSDMELFREVVAGIKERCDVIVQPTTGGGKGMTLEERAAVLPELE
                     PEMATFNTGSFNFGLFPIAERGGDFEEWEIDYLEGTRDYIFRNTFADMEKVCTMFREA
                     GAKPELEAYDVGHLYNIKYLLSRDLLDTPVHIQFVLGVMGANAVTIEQFMHMRRTATE
                     LFGEDYSWSAAGVGYPAEYHLAAMCLMLGGHVRVGLEDNLRVSREKRAESNAELVEKA
                     VALGEMFDREPATPDEAREAFGLKGRAKVAF"
     gene            complement(1477..>2435)
                     /locus_tag="VNT12_02335"
     CDS             complement(1477..>2435)
                     /locus_tag="VNT12_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWI44982.1"
                     /translation="ELEHFDGPGEPGARIPDEILEKVRDRLVSTVGRQMMGDVPVGVF
                     LSGGLDSSLVAAIAARWYAERGEKLKTFAVGLADSPDLLAARAVAEYLGTEHHESVYT
                     AEDALGVLPKVVRTIENFDPSLVRSAVPNYILAAFTAKHVKVVLTGEGADEIFAGYEY
                     LRDFRTEEELHAELVRTIEGLHDLNLQRADRVTMAHGLEARVPFLELDMISLGLSLPA
                     GWKLAGEGQEEKRLLRQAFEGWLPEDFLWRKKAQFGDGSGAASVLKARMEESVTEEEF
                     TRERQEVEPPLRTREEVAYYRIFAEDLGEVSPKRTIGRFATT"
BASE COUNT          427 a          822 c          724 g          462 t
ORIGIN      
        1 tacctcctgc gtggagttgg tcagtgtagc gagcctgaac ccagcgtcgc gcaggcggtc
       61 caggctgtcg ggcacttcag gatgcggcgg caggtcgcgc agtccgccca ggatctcctg
      121 cctatcatcc tcagacacct ccacccctaa ccgctcctcg gtcatcctga gggcagcacc
      181 cgcggcctct ccgaacgtcg tatatgcgtc cgttatggtg gccacgaagg cggactgtag
      241 catctggttg aaccacagcc tccgcacaaa ggtgtcgccg aagacccgct cgaagagcgg
      301 atcgagcgcc gcgaggtcaa gcaaggtctc gttcacatca aagacacaaa ctctcttcac
      361 cacgttcctt tctcaacagc gtccgcaacg gtatcaccct cccgcgaaaa gctgaaatgc
      421 tgaaagcgag ggcctgccag gcccgaagcg tgcagagagg cgcctgcaag gcgccgtact
      481 gactggctaa aacgctacct tggctcggcc cttcaggccg aacgcctcgc gtgcctcgtc
      541 aggggtggcc ggctctcggt cgaacatctc acccagagcc acggccttct ccaccagctc
      601 agcgttcgac tccgcgcgct tttcgcggct gacgcggagg ttgtcttcca ggccgactct
      661 cacgtggccg cctagcatca ggcacatcgc tgccaggtgg tactcagccg ggtagcctac
      721 gccggccgcg gaccacgagt agtcctcgcc gaacagctcc gtcgccgtgc gacgcatgtg
      781 catgaactgc tcgatggtca ccgcgttggc gcccatcacg ccgaggacga actggatgtg
      841 gaccggggtg tcgagcaggt ccctgctgag caggtactta atgttgtaga ggtgcccgac
      901 gtcatacgct tctagctccg gcttcgcgcc ggcctcgcgg aacatggtgc acactttctc
      961 catgtccgcg aaggtgttgc ggaagatgta atcgcgggtg ccctccaggt agtctatctc
     1021 ccactcctca aagtctcccc cccgttccgc gatggggaag aggccgaagt tgaagctgcc
     1081 cgtgttgaag gtggccatct cgggctcgag ctcgggcagg acggcggccc gctcctccag
     1141 ggtcatcccc ttcccgccac ccgtagtggg ctgtacgatc acatcgcacc gctctttgat
     1201 ccccgcaacc acctcgcgga aaagctccat gtccgaactc gggttccccg tctccggctc
     1261 acggacgtgg aggtggacga tggtcgcccc ggcctcgtgc gccccgacgg cggagtcgac
     1321 tatctcatcc ggcgttatag ggatagcctc actctgcccc ggcacggtca ttccgcccgt
     1381 aatcgccgca ctgataatga acttctccac gccagctccc ttcggtagag tcccgaccca
     1441 atactagata cccaccacta cagcagctaa tcgcgctcag gtcgtagcga aacggcctat
     1501 ggtccgcttc gggcttacct cgccgaggtc ttcggcgaag atgcgatagt aggcgacctc
     1561 ctcgcgggta cgtaggggag gctcgacctc ctgccgctcg cgcgtgaact cctcctcggt
     1621 gacgctctct tccatacgcg ccttgagaac cgaggctgcc ccggagccgt ccccgaactg
     1681 tgccttcttg cgccacagga aatcctcagg gagccagcct tcgaaggcct ggcggagaag
     1741 cctcttttct tcctggccct cgccggcgag cttccacccg gctgggaggg agagtccgag
     1801 ggagatcatg tcgagctcca ggaacggtac ccgcgcttcg aggccgtggg ccattgtgac
     1861 gcggtcggcc ctctgcagat tcaggtcgtg taacccctcg atagtccgca ccagttcggc
     1921 gtgtagttct tcctccgtgc ggaaatccct gaggtactcg tagccggcga agatctcatc
     1981 tgccccttcg cctgtcagaa cgaccttgac atgcttggcg gtgaacgccg ccaggatgta
     2041 gttgggtacg gcgctacgta ccagcgaggg gtcgaagttc tcgatagtcc tgactacctt
     2101 gggcaatacc ccgagcgcgt cctccgccgt gtagacgctc tcgtggtgct cggtaccgag
     2161 gtactcggcg accgcgcggg cggccagcag gtcgggagaa tctgcgagtc cgacggcgaa
     2221 ggtcttgagc ttctcgccgc gctcggcgta ccagcgggct gcgatggcgg cgacaaggct
     2281 ggagtcgagg ccgccggaga gaaagactcc gacgggcacg tcgcccatca tctggcgccc
     2341 gacggtactt acaagacggt cgcggacctt ctccagtatc tcatccggaa tcctggcgcc
     2401 aggctcgcca ggcccgtcga agtgttccaa ctcgt
//
LOCUS       DATMJA010000103         4121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_488223, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000103 DATMJA010000000
VERSION     DATMJA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488223"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VNT12_02340"
     CDS             <1..742
                     /locus_tag="VNT12_02340"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HWI44983.1"
                     /translation="RLFAFDVLYAEGERVMQRSYTERHAMLEYAIEAGDTILISEAHT
                     TGEPKELLEVFNDSIQHGLEGIVAKRPDSPYQAGARNYNWVKLKRAQAGNLQDTVDCV
                     IVGYIYGRGRRTAFGVGALLVAAYDPERDVFPTVTKIGTGLSDAQWPEVRERCEPYVS
                     QRKPARVESNIEPSVWVEPQVVIEVLADEITRSPVHPAGADEGGQGYALRFPRLVSFR
                     QEDKKPEDVTTVAEIVELYNQQGARTTP"
     gene            complement(759..2573)
                     /locus_tag="VNT12_02345"
     CDS             complement(759..2573)
                     /locus_tag="VNT12_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWI44984.1"
                     /translation="MDRLPEMETPRYEVLSSLKQVREMLKATERAQLVALDVQAEGTG
                     SVGATLIGVSLCFEPGHVYHAPLGPEGTDSDAILRELRPLLGVSSAPKVMHDSKPGLV
                     VLAQRDVEPGAPSFDTLLAAFLLDGKAYALEDLITERLGLTAGESKAREGQDRTRNLC
                     ARVDAVLRLSGLLEAELEERGQLAYLRELEMQLVPVLADMELAGVAVDTAALEDLSLR
                     LAGKLRDLEREIAEVAGHEVDPRSPRRLGELLYEKLGLEGSRRTSSGRYSTDALALAA
                     LAGQHPVVEKIVEHRRLSRLKDAYADSLPGLVNPQTGRVHTNFSQVSASSGRLVSREP
                     NLQNIPVRTQLGREIRNAFVADASGLGIKGPTVLLSVDYSQIELRVLAHMTGEPVLRE
                     AFQKGEDVHALAASCLYGVPPEEVTAEMRRIGKMLNYGVIYGMTGYRLALEAGIDRKE
                     ATRFVKRHREQYPAVRAYVERTLREAEEKGYVETPLGRRRYLPDLGAKNRQRREAARR
                     AAENMPNQGMVAEIIKLAMIRVHARLRKEGIASRMLLQVHDELVLEVPEVELVEAAGL
                     VVREMKHAVELSVPLEVEVKIGRSWGELRKLEDARVNQ"
     gene            2759..3745
                     /locus_tag="VNT12_02350"
     CDS             2759..3745
                     /locus_tag="VNT12_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWI44985.1"
                     /translation="MRGDHDRDVGQKRHSFHAVRREAFNYVWDSSIEPALEVDSGDLV
                     EFTVRDASDEQIQADSTSEDVAKLDFEHVNPVSGPVYVKDARPGDVLAIEILEFQPMD
                     WGWTALIPGFGLLADEFPEPWLRISWVEAERSKVVFAEGIELPYVPFPGTIGVAPAEP
                     GPHSMVPPSRWGGNMDTKHLRVGTTLYLPVGVEGALFSVGDTHSAQGDGEVCGTAIET
                     AMEVSLRLSVRRDFHIEAPQYRVPPSALAGAEGTGYHVSTGVAPDLMEATRQAVRATI
                     EHLGDRHGLSREEAYALCSVAVDLRIHEVVDAPNWVVGAFLPDDIFTGGGGR"
     gene            3742..>4121
                     /locus_tag="VNT12_02355"
     CDS             3742..>4121
                     /locus_tag="VNT12_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44986.1"
                     /translation="MSTEADSAPERGLRRELRFWEAIALSIGIMAPGAAMALNGTLPA
                     SLVGRAVPLAFVFASVGILFVSYAVIRLTGYFNHAGSVYALSGATLGPRAGFFSGWAL
                     LGTYLAFTAASTAEVGLFGQAFLDG"
BASE COUNT          691 a         1359 c         1293 g          778 t
ORIGIN      
        1 gaggcttttc gccttcgacg tcctctacgc cgagggcgag cgtgtcatgc agcgatctta
       61 caccgagagg cacgccatgc tcgaatatgc gatcgaagcg ggggatacca tccttatctc
      121 cgaagcccat acgaccggcg aaccgaagga gctgctcgag gtgttcaatg attccatcca
      181 gcatggcttg gagggcatcg tcgccaaaag gcccgactca ccctaccagg ctggggcgcg
      241 caactacaac tgggtcaagc tcaagcgcgc acaggcgggc aacctccagg ataccgttga
      301 ctgcgtcatc gtcggctaca tctacggccg gggtcggcgg accgcgttcg gcgtgggcgc
      361 actactcgtt gccgcctacg accctgagcg cgacgtcttc ccgaccgtaa ccaagatcgg
      421 gaccggcttg agcgacgccc agtggcccga ggtgagagag cgctgcgagc cgtacgttag
      481 ccagaggaag cctgcgcgcg tcgagagcaa catagagccc agcgtgtggg ttgagccgca
      541 agtggtcatc gaggtgctcg ctgacgagat cacccgctcg cccgtgcacc ctgcgggagc
      601 ggacgaaggg ggtcagggct acgctctgcg gttcccgcgt ctcgttagtt tccgccagga
      661 ggacaagaaa cctgaagacg tcaccacggt cgccgagatc gtggagcttt acaaccagca
      721 gggggcgcgg acgacgccgt aagtgtggcg tcgccccgct actggttcac cctggcatcc
      781 tctagctttc gcagttctcc ccacgatcgc cctatcttga cctcgacctc cagcggcacg
      841 gagagctcca cagcgtgctt catctcgcgt accaccagcc cggccgcctc gaccagttcc
      901 acttcgggga cctccaggac gagctcgtcg tgaacctgca acagcatgcg cgacgctatg
      961 ccctccttgc gcagacgcgc gtgcaccctg atcatcgcca gcttgatgat ctccgcgacc
     1021 atgccctggt tcggcatgtt ctctgcggcg cgccgtgctg cctcccggcg ctgcctgttt
     1081 ttggctccga ggtcgggcag gtaccggcgc ctccccaagg gggtctctac atagcccttc
     1141 tcctctgctt cgcgcagtgt cctctcgacg taggcccgca cggcaggata ctgctccctg
     1201 tggcgcttga cgaaccgcgt ggcctccttc cggtcgatgc ctgcctcgag cgcgagcctg
     1261 tagccagtca tcccgtagat gacgccgtag ttgagcatct tgccgatccg acgcatctcg
     1321 gcggtgactt cttctggcgg cacaccgtag aggcaggagg cggcgagggc atggacgtcc
     1381 tcgcccttct ggaaggcctc acgcagcacc ggctccccgg tcatgtgtgc cagcacccga
     1441 agttctatct gggagtagtc cacgctaagt agcacggtcg gcccttttat tccgaggccc
     1501 gaggcgtcag ccacaaacgc gttgcggatc tcgcgcccga gctgggtgcg cacggggatg
     1561 ttctggaggt taggttccct ggagacgaga cggccactgc tcgccgagac ctggctgaag
     1621 ttcgtatgca cgcgtcccgt ctgcgggttt actagaccgg gaagcgagtc ggcgtaggcg
     1681 tccttgagcc tgctgaggcg ccggtgctcc actatcttct ctacaaccgg gtgctggccg
     1741 gctagcgctg cgagcgcgag cgcgtccgtc gagtagcgcc ccgaagaggt cctcctgcta
     1801 ccttccaggc ccagcttctc gtaaagaagc tccccgagtc gccgcggcga cctcgggtcg
     1861 acctcgtgcc cagccacctc agcaatctcc ctctcgagat cgcgcagctt ccccgccagg
     1921 cggagtgaca gatcctcgag cgcagcagta tcgacggcga cgcccgcgag ttccatgtcg
     1981 gcaagtacag ggacgagctg catctcgagc tcacgcaagt acgcaagttg acctcgctcc
     2041 tcgagttccg cctccaggag cccgctgagg cgcagcaccg catcgacccg agcacagagg
     2101 tttcgcgtcc tatcctgacc ttctcttgcc ttactttctc ccgccgttag gccgagcctc
     2161 tcggtgatca ggtcctctag agcgtatgct tttccgtcga gaaggaaagc cgcgagcaga
     2221 gtgtcgaagc tgggcgcgcc aggctccaca tcacgctggg cgagtaccac gagcccgggt
     2281 ttggagtcgt gcatcacctt aggcgcggat gacacgccta gtagcgggcg aagctcccga
     2341 agaatcgcat cgctgtccgt cccttcgggg cccagaggcg cgtggtagac atggccgggc
     2401 tcgaagcaga gcgaaacacc tatgagggtg gccccaactg accccgtccc ttcagcctgg
     2461 acgtcaaggg ctactagttg tgcgcgctcg gttgccttta gcatctcacg cacctgtttc
     2521 agggacgaaa gcacctcgta gcgcggcgtc tccatttctg gcagccggtc catctgaccc
     2581 gtgcaagcac ctcacttttc ctcagcagta gtcgtccgcc gcaactacca ttgtaccgtc
     2641 ggttgggcca cgcccggaac caattgggcg cttgctcgtc ggacactctg ctatagtggc
     2701 cctcccggca ggagcacagg cggctagggg accaggccgt ggcggaagga gaatggcaat
     2761 gcgaggcgat cacgaccgcg acgtgggaca aaagcggcac tcttttcacg ctgttcgccg
     2821 ggaggctttc aattacgttt gggacagcag catcgagccc gcgctcgagg tggactcggg
     2881 cgacctcgtc gagttcaccg tgcgcgacgc ctcggacgag cagatccaag ccgactccac
     2941 ctcagaggac gtcgccaagc tcgacttcga gcacgtcaac ccagtcagcg ggcctgtcta
     3001 cgtcaaggat gctcgacctg gggacgttct ggccatcgag atccttgagt tccagccgat
     3061 ggactgggga tggacggcgc ttatccctgg ctttgggctt ttggccgatg agttccccga
     3121 gccgtggctc cgaatctcgt gggtggaggc ggagcggagt aaggtggttt ttgcagaagg
     3181 gatcgagctg ccctacgtgc ccttccccgg aacgatcgga gtcgccccgg cggagcccgg
     3241 acctcactct atggtgcctc cctcacgttg gggcggcaac atggacacca agcacttacg
     3301 agtcggcaca accctctacc tgccggtcgg ggtcgagggg gcactcttct cagtcgggga
     3361 cacccactcg gcgcagggag acggagaggt ctgcggaacg gccatagaga ccgcgatgga
     3421 ggtctccttg cgcctctcgg ttcgtcgcga ctttcacata gaggcgcccc agtatcgtgt
     3481 gccgcccagc gcgcttgccg gggcagaggg caccggctac cacgtctcga cgggggtggc
     3541 acccgaccta atggaggcga ccaggcaggc ggtacgggct actatagagc acctgggcga
     3601 tcgtcatggt ctctctcgcg aggaagctta cgcgctgtgc agcgtcgccg tggacctgcg
     3661 catccacgag gtggtagacg ctcccaactg ggttgtcgga gcgttcctgc ccgacgacat
     3721 cttcaccgga ggaggtgggc gatgagcacg gaagccgact cggctccgga gcgtggtttg
     3781 cggcgcgagc tgcgcttctg ggaggcgatc gcgctgtcga tcggcatcat ggctcctggt
     3841 gccgccatgg ccctgaacgg tacgctgccg gctagtttgg tggggcgggc agtcccgctg
     3901 gcgttcgtct tcgcctccgt aggcatcctg ttcgtctcct acgccgtcat ccggctcacg
     3961 ggctacttca accacgcggg gtctgtctac gccctctccg gcgcgaccct cggcccccgc
     4021 gccggtttct tctccggctg ggcgctcctg ggcacttact tggccttcac ggccgcatcc
     4081 accgccgagg tgggtctctt cgggcaggcc ttcttggacg g
//
LOCUS       DATMJA010000104         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_14305, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000104 DATMJA010000000
VERSION     DATMJA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14305"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..669
                     /locus_tag="VNT12_02360"
     CDS             307..669
                     /locus_tag="VNT12_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWI44987.1"
                     /translation="MKSCLKMLLVAGAFAVSLAAFQDDALAETQLASWYGPGLEGNLT
                     ASGDVFEPYTEYTAASPYLPFGTDLLVTYGGYSVVVEVTDRGPYVGGRDLDLSAAAAE
                     EIGLTYAGVDYVDVEVLN"
     gene            874..1395
                     /locus_tag="VNT12_02365"
     CDS             874..1395
                     /locus_tag="VNT12_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWI44988.1"
                     /translation="MEETLRGIRFFEFLTEEEREEFAEAAESVSFGPGENIIEEGADQ
                     ESLFVLVSGAVEVRKGLPGGRSRLLAEIDASRGRTVVGERGLLGESGASATVRTVDEV
                     EAIRIPRDTFRRMVAEGRPAAFKLSYRIARTLARRLTRLDEEVVEAIREIENRGETDM
                     EAFRDRLITDWSV"
     gene            1409..1933
                     /locus_tag="VNT12_02370"
     CDS             1409..1933
                     /locus_tag="VNT12_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWI44989.1"
                     /translation="MRQTPDLSEVPIFKHLDEDERAEFEELSEPGRFQAGQTIFEEGA
                     PEERLYVIISGTVEVYKSVLPGRRQRLATIEAPTVVGEMGLLTEPRAAASVEAMTMVE
                     AHGVGRDQLLEMLDADSPAACKLVYEIGRTLAERMAATNETVAEVIFRLENTAGRSED
                     IDVFRDQLIRDWSF"
     gene            complement(2004..2567)
                     /locus_tag="VNT12_02375"
     CDS             complement(2004..2567)
                     /locus_tag="VNT12_02375"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWI44990.1"
                     /translation="MAVAQRNEAPGELELVRRFVNTRDVEEGTDELDGPESLLGWFSG
                     TGLLDDEAIADEEDLKRSLALREGIRSLLLANNGEGVEPADLRELNRVAESVCLRVRF
                     DENGGPTLGPQSSGVSAALGRILAAVVRATDEGIWGRLKVCTNDACQWAFYDRSKNRS
                     GKWCTMEVCGNRMKARAFRQRQGAGEG"
     gene            complement(2622..2825)
                     /locus_tag="VNT12_02380"
     CDS             complement(2622..2825)
                     /locus_tag="VNT12_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44991.1"
                     /translation="MHDNLTRLEVLRQHREELLQEAENARLAREARRGRRGSGREWRI
                     SLGGWILQLRRIPEYEASQAAKS"
     gene            complement(3009..>3525)
                     /locus_tag="VNT12_02385"
     CDS             complement(3009..>3525)
                     /locus_tag="VNT12_02385"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWI44992.1"
                     /translation="DLLDWGRQAGLLALEETEDLVRQATLDPEAAQEILSRALALREE
                     IHRVISRAIAGESQDVSDLSALNGELSIALSHLRVMPADGAYTWGWDRSGDDGGARLD
                     SPLWPVAQSAAELLTSPKLGRVKLCAGEGCGWVFLDESRNGSRRWCDSRDCGNRERVR
                     RHLARKRASDS"
BASE COUNT          663 a         1098 c         1095 g          669 t
ORIGIN      
        1 ggaaagatgc cccgtggagc ccaacgctcc gccgaccgta agcttcccga gttcgaggct
       61 cgcatccaac gctacaggcg ctcgctcgac aaagagcgat agacccagaa gttcaaacca
      121 cgcctcacct agcaaaaacc cccatatgta ggtaaaaagc gtcgctgtca ccacgtagcg
      181 aaggtccgcc gtctatgtaa ctggttcaca tatagacgca aaaaacgata tgtagcggct
      241 tgtaactggg gtgtaacgta gtagggtctt gggcttgttg tgcaagcgat ctggaggggg
      301 gactctttga agtcttgtct caagatgctg ttggtggccg gtgcgttcgc cgtatcgctg
      361 gccgcctttc aggatgatgc cctggccgag acccagctgg cgagctggta cgggcccggt
      421 ctcgagggca acctgactgc gagtggggac gttttcgagc cctacaccga gtacacggcg
      481 gcgtcaccgt acttgccttt cggcacggac ttgctggtga cctacggagg ctactccgtg
      541 gtcgtagagg tcactgaccg tgggccgtac gttggcggca gggacctgga cctctccgcg
      601 gctgctgccg aggagatcgg gctcacctac gctggtgtcg actacgttga cgtcgaggtc
      661 ctcaactagg ggtcttcgcc agactaacgg cgaggcacgt cagcgggcgc ggctaaaatg
      721 ctgcgcccgt ttccctttgg gctaatcgtc acacccttcg gtatcccatg taacgaagaa
      781 gatctttcga tgggcttttc gcacggagcg ccgggacgga aggcgcgcgt ataatctcgc
      841 caagggcttc gaggttgaag gggtgatggg ataatggagg agacgcttcg ggggatcagg
      901 ttcttcgagt tcctgaccga ggaggagcgg gaggagtttg cggaggcagc ggagtccgtc
      961 tccttcgggc cgggcgagaa catcatcgag gagggcgcag atcaggagag tctgttcgtg
     1021 ctcgtctcag gcgcggtgga ggtcagaaag ggattgccag gaggaaggag caggctcctg
     1081 gccgagatcg acgcctcacg cgggcggacc gtcgtggggg agagggggct tctcggggag
     1141 agcggggcgt ccgctacggt caggacggtc gatgaggtcg aggcgataag gatcccacgc
     1201 gacactttca ggcgcatggt cgcagaaggc aggcctgccg ccttcaagct ctcctaccgc
     1261 atcgcccgca ccctcgcgag gagacttacg cgtctggacg aggaggtggt cgaggcgatc
     1321 cgggagatcg agaacagggg ggagaccgac atggaggcct tccgcgacag gctgataaca
     1381 gactggtccg tataggggag ttctttttat gcgacagacg ccagacctca gcgaggtgcc
     1441 gatcttcaag cacctggacg aggacgagcg ggccgagttc gaggagcttt cagagccggg
     1501 aaggttccag gccgggcaga cgatcttcga ggaaggcgcc ccggaggagc ggctgtatgt
     1561 gatcatctct ggaaccgtcg aggtttacaa gagtgtcctc cccggacgcc ggcaacgcct
     1621 ggcgaccatc gaggcgccca ctgtggtcgg cgagatgggc ctcctcacag aacccagggc
     1681 cgccgcgagc gtcgaagcga tgacgatggt cgaggcgcac ggcgttggta gggaccagct
     1741 tctcgagatg ctcgacgccg actcccccgc agcctgcaag ctcgtctacg agatcggccg
     1801 taccctcgcc gaacgtatgg ccgcgaccaa cgagaccgtc gccgaggtca tcttccgcct
     1861 cgagaacacc gccgggagat ccgaagatat agatgtcttc agggaccaac tcatccggga
     1921 ctggagcttc tagagcattt cagcacgctc cagctggagc gtgcttactt gcctcccccc
     1981 gtgctcgtta ctctaggacc ggattatcct tcgccagcac cctgacgctg ccgaaaggcg
     2041 cgggccttca tgcggttgcc gcagacctcc atcgtgcacc acttgccgga gcggttcttt
     2101 gagcggtcgt aaaaggccca ctggcacgca tcgttggtac agaccttcaa cctgccccat
     2161 atcccctcgt ccgtggcccg caccacggcg gccaggatac gaccaagggc cgcggacacg
     2221 ccggacgatt gaggcccgag cgtagggccg ccattctcat cgaaacggac cctaagacac
     2281 acgctctcag ctacgcggtt gagctccctg aggtcggccg gttcgacccc ttcaccattg
     2341 ttggccaaga gcagggagcg tatcccttct cgcaatgcga gcgagcgctt caggtcttcc
     2401 tcatccgcga tcgcctcatc atcgagcagg cccgtgcctg aaaaccagcc gagtagggac
     2461 tcagggccgt cgagttcgtc tgtgccttct tcgacgtccc gggtgtttac gaaccggcgg
     2521 accagctcca actcgcccgg cgcttcgttt ctttgcgcaa ccgccatatc ttctccacct
     2581 actgcaggta tgcgacttta cgaccgctag ctttgagctt ttcagctctt cgcggcctgg
     2641 ctcgcctcgt actccgggat cctccgcagc tgcaggatcc atccccccag acttatccgc
     2701 cactccctgc cagatcctct ccgcccgcgg cgggcctccc tcgcgaggcg ggcgttctcc
     2761 gcctcctgga gcagctcttc gcggtgctgg cgcaatacct cgagtcgggt caagttgtcg
     2821 tgcatcttcg ccccttttca cgctccgtgg aggccgtagc cccgcctctt ctaactacca
     2881 taaatgatac actggttatg gtaactagtc aatagttttt gctagttatt tttcggagga
     2941 gagggccgga accctgcaaa agcaggagaa tcggcccgag actttcaaat ggagaacgct
     3001 aagaaccgtt acgaatcgga ggctcgcttg cgagcgaggt ggcggcgtac ccgctcccgg
     3061 ttgccgcagt cgcgagagtc acaccacctg cggctgccgt tgcggctctc gtccaggaat
     3121 acccagccgc agccctcccc ggcgcagagt ttgaccctgc ccagcttcgg ggatgtcaag
     3181 agctccgccg cagattgcgc cacgggccac aaggggctgt cgagccgcgc ccctccgtca
     3241 tccccgctcc ggtcccagcc ccaagtgtag gcgccatcag cgggcatcac gcgtaggtgc
     3301 gagagcgcga tcgagagctc gccgttgagc gccgacaggt cgctcacgtc ttgggattca
     3361 ccggcgatgg ccctggagat cacacggtgg atctcttctc gcagggcaag cgctcgcgag
     3421 agtatctcct gtgccgcctc tgggtccagc gtcgcctgcc gaacgagatc ctccgtctcc
     3481 tccagagcaa gcaaaccagc ctgtctgccc cagtcaagca agtcc
//
LOCUS       DATMJA010000105         3420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_251037, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000105 DATMJA010000000
VERSION     DATMJA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251037"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VNT12_02390"
     CDS             <1..193
                     /locus_tag="VNT12_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWI44993.1"
                     /translation="YQQAFEDALNNCSTEHAPWYIVPANKKWYRNLVVARTIADTLVA
                     MDPQYPPAEEGLEKVTIPD"
     gene            complement(228..1184)
                     /locus_tag="VNT12_02395"
     CDS             complement(228..1184)
                     /locus_tag="VNT12_02395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWI44994.1"
                     /translation="MPQKVLVTREIPSAGLRLLRDFDVTVVSESPPDHGELLEAVRGA
                     AGILPTVTEKMDAEVMDAAGDGLKVIANMAVGYDNVDLDAAGERNVVVTNTPEVLNET
                     TADTAFMLLLAAARRLGEAERLLRSGGWEAWGPMQLTGPDVWGKKLGVVGFGRIGQAV
                     ARRARGFDMEVLYHDQYRNESAENETRVRYVELDELLRTCDFISVHTPLTDETYHLIG
                     ATELGRMKPEAVLINTSRGPVVDEAALADALAEERIFAAGLDVYEKEPEVHPKLLELE
                     NVVLAPHIGSASIETRDKMAALAAENLAAVLRGEEPKTPVNE"
     gene            complement(1206..>3420)
                     /gene="acnA"
                     /locus_tag="VNT12_02400"
     CDS             complement(1206..>3420)
                     /gene="acnA"
                     /locus_tag="VNT12_02400"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HWI44995.1"
                     /translation="GFKLTGALQEGVTATDLVLTVTQMLRAHGVVGKFVEFYGDGLSR
                     LSLPDRATIANMSPEFGATCTFFPVDTETLRYLRGTGRDEQLVDLVEAYSKEQGLWRT
                     DETPEPRFSETLNLDLESVEASLAGPRRPQDRVALDDMQPSFRQALAEMVNPDHPLLK
                     NGEDDAYNHADEESFPASDTPNPDIPVARGGTNEAEGKEGGAVSTGESHTSDDTKSFP
                     ASDTPAGAANVTNEGGEDPTSGGEPENGKPEAGPSGAITVTVDGEELYLRHGSAVIAA
                     ITSCTNTSNPSVMIGAGLLARKAVEKGLSVAPHVKTSLAPGSKVVTEYLQTSGLLNPL
                     ERLRFDVVGYGCTTCIGNSGPLAEEISAAVEENDLVVAAVLSGNRNFEGRINPDVRAN
                     YLASPPLVVAYALAGTVDIDLSKDPIGEDEDGNPLYLEDIWPSQEEVAREVDNALDPK
                     IYKEQYADVYTGNEQWNDVEVPSGDLYEWDPDSTYIQEPSFFKDMGPEAEDLKDIEGA
                     RILVKVGDSVTTDHISPAGAIPSKMPAGQYLIQKGVDPRDFNSYGSRRGNHEVMVRGT
                     FGNIRLRNQLVDKEGGYTLHFPDGEETTIYEASLKYDEENVPLVVLAGKEYGSGSSRD
                     WAAKGSFLLGVKAVIAESFERIHRSNLIGMGVLPLQYAEGESAQSLGLSGQESFDISG
                     LAGLEPGKQLTVKVTDDEGEEKEFEVKARVDSPVEVEYLRNGGILQTVLRQMLEE"
BASE COUNT          523 a         1100 c         1112 g          685 t
ORIGIN      
        1 ctaccaacag gccttcgagg acgccctgaa caactgttcg accgagcacg ccccctggta
       61 catcgtgccg gccaacaaga agtggtaccg caacctcgtc gtcgctcgca ccatcgccga
      121 caccttagta gcaatggacc cacaataccc tccggcggaa gaagggttgg agaaggtaac
      181 gataccggac tagccctcct cgaaccggct ccgccaagcg cttacagcta ttcgttgacc
      241 ggtgtcttcg gttcctcgcc gcgcagcacg gcggccaggt tctccgctgc gagggcagcc
      301 atcttgtcgc gggtctcgat ggaggcgctg ccgatgtgcg gcgcgaggac gacgttctcc
      361 agttccagga gcttggggtg tacctcgggc tccttctcgt acacgtcgag gcctgcggca
      421 aagatacgct cctcggcgag ggcgtcggcc agggccgctt cgtccacgac ggggccgcgc
      481 gaggtgttta tgaggacggc ttcgggcttc atccttccca gctccgtcgc gccgatgagg
      541 tggtaggtct cgtcggtcag cggggtgtgg acggagatga agtcgcacgt gcggagtagt
      601 tcgtcgagct cgacgtaccg caccctggtc tcattctctg cgctttcgtt gcgatactgg
      661 tcgtgataca ggacctccat gtcgaagccc cgggcgcggc gggctacggc ttgtccgatc
      721 ctgccgaacc ctacgacccc gagcttcttg ccccagacgt cgggacccgt gagctgcata
      781 ggtccccagg cctcccaccc gccggagcgc aggagacgct ccgcctcgcc gagtctcctg
      841 gccgccgcga gcagcagcat gaaggctgtg tcggcggtcg tctcgttcag gacctcgggg
      901 gtgttggtca cgaccacgtt ccgctctccg gcggcgtcga ggtctacgtt gtcgtagccg
      961 acggccatgt tggcgataac cttcagcccg tctccggcgg cgtccatgac ttccgcgtcc
     1021 atcttctcgg tgacggtcgg taggatgccg gctgcaccac ggacggcttc gaggagctcg
     1081 ccgtgatctg gcggactctc tgaaacgacg gtgacgtcga agtctcgaag gagacggagc
     1141 ccggcagacg ggatctcacg ggtgacgagt accttctggg gcatggcacc tccctacttc
     1201 gggctctact cctcgagcat ctggcgcaat acggtctgca ggatgccgcc gttacggagg
     1261 tactcgacct cgaccgggga gtccacgcgc gctttcacct cgaactcctt ctcttcgcct
     1321 tcgtcgtccg tgaccttcac ggtcaactgc ttgccaggct cgaggccggc aaggcctgag
     1381 atgtcgaagg actcctggcc gctcagcccg agtgattgtg cgctctcgcc ttcggcgtac
     1441 tgcagcggaa ggacgcccat gccgatcagg ttcgagcggt ggatgcgctc gaagctctcc
     1501 gcgataacgg ccttgacgcc gagcaggaac gagcccttgg ccgcccagtc gcgcgatgag
     1561 ccggacccgt actccttgcc ggccaggacg acgaggggca cgttctcctc gtcgtacttc
     1621 aggctcgcct cgtagatcgt ggtctcctcg ccgtcgggga agtggagggt gtaaccgccc
     1681 tctttgtcca cgagctggtt gcggagcctg atgttgccga acgtgccgcg caccattacc
     1741 tcgtggttgc cgcgtctgga accgtaggag ttgaagtcgc gcgggtctac gcctttctgt
     1801 atcaggtact gccccgccgg catcttcgaa gggatggcgc cggcgggtga gatgtggtcg
     1861 gtggttacgg agtccccgac tttcacgagg atgcgcgcgc cttcgatgtc tttcaggtct
     1921 tctgcctcgg gccccatatc cttgaagaac gagggctcct ggatgtaggt cgagtcggga
     1981 tcccactcgt agaggtcgcc tgatggcacc tctacgtcgt tccactgctc attgcccgtg
     2041 tacacgtcgg cgtactgctc tttgtagatc ttagggtcga gggcgttatc tacctccctg
     2101 gccacctcct cctgtgaagg ccagatgtcc tcgaggtaca gggggttgcc gtcctcatcc
     2161 tcgcctatag ggtctttgga caggtcaatg tccaccgtgc ctgccagggc gtaggccacg
     2221 acgagcggcg gggaggccag gtagttcgcg cggacgtcgg ggttgatgcg gccctcgaag
     2281 ttgcggttgc ccgagagtac ggccgcgacc acgaggtcgt tctcctcgac ggccgcggag
     2341 atctcctcgg ccagcggacc ggagttccct atgcatgtcg tgcacccata gccgacgacg
     2401 tcgaacctga gccgctccag gggattcaga agccccgagg tctgcagata ctccgttacg
     2461 accttggacc ctggcgcgag gctggtcttg acgtgcggcg caacggagag ccccttctcc
     2521 acggccttcc ttgccaggag gccggcacct atcatcacgg aaggattgga ggtattggtg
     2581 caactcgtta tggcggcgat cacggcggag ccgtgcctga ggtagagttc ctccccgtcc
     2641 accgttacgg tgatggcgcc agatggtccg gcctcaggct tgccattctc gggctcgccg
     2701 ccggatgtcg ggtcttcccc accctcgttt gtgacgttgg cagcgccagc cggggtgtcg
     2761 ctagccggga aggacttggt gtcgtctgag gtatggctct cacctgtgga gacggcgccg
     2821 ccctccttgc cctcggcctc gttggtcccg ccgcgggcga ccggtatgtc ggggttcggg
     2881 gtatcgctcg cggggaagga ttcctcgtcg gcgtggttgt acgcatcatc ttcgccgttc
     2941 ttgaggagcg gatgatcggg gttcaccatc tcggccagcg cctgcctgaa ggagggctgc
     3001 atgtcgtcga gggcgaccct gtcctgcgga cggcgcggcc cggcaaggct cgcctcgacc
     3061 gactccaggt ccagattcaa cgtctccgag aagcgcggct cgggcgtctc gtccgtgcgc
     3121 cagaggccct gctccttgct gtacgcctcg accaggtcca caagctgctc gtcgcggccc
     3181 gtgcccctaa ggtagcgtag cgtctcggtg tctacgggga agaacgtgca cgtcgccccg
     3241 aactcggggg acatgttcgc gatggtggca cggtcgggga gggagagcct gctcagccca
     3301 tcgccgtaga actccacgaa cttgccgacc acaccgtggg cgcggagcat ctgggtgacg
     3361 gtcaggacga ggtccgttgc cgtgacgccc tcctggaggg ctccggtcag cttgaaaccg
//
LOCUS       DATMJA010000106         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_725381, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000106 DATMJA010000000
VERSION     DATMJA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725381"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1560
                     /locus_tag="VNT12_02405"
     CDS             151..1560
                     /locus_tag="VNT12_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HWI44996.1"
                     /translation="MIDLFGILISAMPFVVAAVGLAGLGWSASTAGGASLAVAVLGAV
                     VWPDLEGTAVPGALFAGLGTSAQVLYVLFGGLLLYNLLSAGGAVDSVSRFLGGLEPDR
                     VALAAGVVVGVAPFFESVTGFGVAVVISAPILLAAGFTPLRAAVLASWGQCAVPWGAL
                     GVGTVIGAHLAGMGFGTLSDVSAFLSIPLFPVYGVAAVALAGGWTGVRRRGAEAIALG
                     LVAGAGTLLTSLYLVPELSGAVGGLAATALFLARRRNRLAGVPVRALLPYAFLLALLA
                     AANDIGALRSVLEELGPVFDGPGLPLLASGAFAALLLGLGGNEIGAATRRTVTQWLPT
                     AAAVLTFVLAGEVVAESGAAGLLAAGAEALGVFFPTVSPVLAALGGAITGSNAASNAL
                     FMPLQVEAARELGISEVLTAAVQNVAGSHASMLAPQRLVLAATATGLLGEEGDILRAS
                     VAPVTVSIGLLAMLGMLLS"
     gene            complement(1647..1820)
                     /locus_tag="VNT12_02410"
     CDS             complement(1647..1820)
                     /locus_tag="VNT12_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI44997.1"
                     /translation="MVMQEICDNEEIGFEGGQYASTEEELYEKAIALLEMCKIESLHS
                     GQKITEPTAETRQ"
     gene            2003..2254
                     /locus_tag="VNT12_02415"
     CDS             2003..2254
                     /locus_tag="VNT12_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HWI44998.1"
                     /translation="MGDKPDRDVEKIYSTSEFVAKLRRLADALEAGGRFEIQVAGERV
                     YVPSRAEFNIEHEREGDEEEIEFQLKWTNSSSARSDPAR"
     gene            2545..>2894
                     /locus_tag="VNT12_02420"
     CDS             2545..>2894
                     /locus_tag="VNT12_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWI44999.1"
                     /translation="MDSRFTRRRFLTAAGAAAAYLALTPTVGCQPPEHSSKVRSSRDP
                     NVEPLPDAPFPQDGVWAFRSRPDLDPPVVEVITEAHDDTAPGYIFVAPEKGGTGKGGS
                     MIIDDRGQVVWFHPL"
BASE COUNT          440 a          884 c          978 g          592 t
ORIGIN      
        1 cagagagctc agccaactcc tccagcgtca gacctcgacc acggcgtaac tcccttactc
       61 taccccccaa ccgcccggaa gagaccgcag cgaccatcgt agaacccccg cttgcagccg
      121 tatcgtccat ttggtagaag gtattctacc atgatagatt tatttgggat cttgatctcg
      181 gcgatgcctt tcgtggttgc cgctgtgggt ctggcgggcc ttgggtggtc tgcgtccacg
      241 gctggtggtg cgtcgctggc agtggctgtg ttgggggcgg ttgtgtggcc ggatctggag
      301 ggcaccgcgg tgcctggtgc tctatttgcg gggctaggga cctcggccca ggtactgtac
      361 gtgctgttcg gggggttact gctctacaac ctactttcgg caggaggggc tgtcgacagt
      421 gtgtcgcgct ttctcggcgg gctggagcct gaccgcgtgg cgctcgcggc cggagttgtg
      481 gtcggggttg cccctttctt cgagtccgtt acgggattcg gggtggctgt ggtcataagc
      541 gccccgatcc tgctggccgc ggggttcaca ccgcttaggg ccgccgtgct tgcgagctgg
      601 ggccagtgtg ccgtgccatg gggcgccctc ggtgtcggga ccgtgatcgg ggcccatctt
      661 gcagggatgg gcttcgggac actctccgac gtgagcgcat tcttgagcat ccccctcttc
      721 cccgtctacg gggtcgccgc cgtcgcgctt gcgggagggt ggacgggcgt ccgccgacgc
      781 ggcgcggagg cgatagcgct ggggctggtc gcaggggcgg ggacgctcct tacgagcctg
      841 tacctggtgc ctgagctctc cggcgccgtc ggaggacttg ctgccacggc cctcttcctg
      901 gcgaggaggc gtaaccggct cgcaggagtt cctgtgcgcg cgctgctccc ctacgctttc
      961 ctgctcgcgc ttctcgcggc ggcgaatgat atcggggcgt tgcggtcggt tctcgaagag
     1021 ctaggtcccg tcttcgacgg tccaggcctc ccgttgctcg cgtcaggtgc gttcgcggcg
     1081 ctgctgctgg gtctcggcgg aaacgagatc ggtgctgcca cgcggagaac cgtgacgcag
     1141 tggcttccga ccgcagcggc cgtcctgacg ttcgtcctgg cgggggaggt ggtggcagag
     1201 agcggtgcag cggggttact cgccgcaggc gcagaagccc ttggggtctt ctttcccacg
     1261 gtctcgcccg tgctcgcggc ccttggcggc gccatcaccg gttccaacgc ggcctcgaac
     1321 gcgttgttca tgccgctcca ggtcgaggct gcgcgggaac tcggcatatc tgaggttctc
     1381 acggctgcgg ttcagaatgt cgccgggagt cacgccagca tgctcgcccc gcagcgcctg
     1441 gtgctcgcgg caacggccac tggcctgcta ggcgaggaag gggacatcct gagggcttcg
     1501 gtcgcccccg taactgtctc catcggcttg ctcgcgatgc tcgggatgct acttagttag
     1561 ggcgttcggc gagacgaccg taccgactcg cctcttccgg ccaatttggg aattctgccg
     1621 gttctgggcc cgtgggcgtt acacggttat tggcgggttt cggcggtggg ctccgttatc
     1681 ttctggcccg aatgcaggct ttcgatcttg cacatctcga gaagagctat ggccttctcg
     1741 tagagctctt cttcagtact ggcgtattga ccgccctcga agcctatttc ctcattgtca
     1801 cagatttcct gcatcaccat gaggctacac gttgcggccc gtcttcgggt tacaggagtg
     1861 caacatcttg gtaaaagcct gataacctgg ccgagagttc gacctgacaa tgactcctgc
     1921 atcaacccgg tccgacttgg cttaggctct ctgacccttt aagctacgga gaggtcgagg
     1981 gttcgcgaac aagggaggat ggatgggcga taagccagac agggacgtcg agaagatata
     2041 ctccacctcg gagttcgtgg cgaagcttag aaggcttgcc gatgcactgg aagcgggcgg
     2101 gcggttcgag atccaggtcg ccggggagag agtctatgtg ccctcccgcg ccgagttcaa
     2161 catagagcac gagcgtgagg gggacgagga ggagatcgag ttccaactca agtggaccaa
     2221 ctcctcctct gcccgttccg acccggcacg ctgacggtca gatcccgtgg gagactcgta
     2281 aggagccagc cgcgcgagga ccccggcagg cgaagcctcc ttgcgattgc agggaggttt
     2341 cgctcttcca tgagagggcg cgtcgggatc tggcatggtg cacactgtac atcggagtga
     2401 gagctaccct aggaggaccg catgacggga ccgcaccaga cgacatcgtt ctgggctatg
     2461 agggcatcat aggcgggcag gagcgccccc tcgcctcacg gggtcacatt cgcggcggag
     2521 tgctcgtggt gaagggcgcc agcgatggac tctaggttca cgcggcgccg gtttctaacg
     2581 gcggcgggcg cagccgccgc ttatctggcg ttgaccccca cggtgggctg ccagccgccc
     2641 gagcactcct cgaaggtgag atcctcgcgc gacccaaatg tcgagccctt gccagacgct
     2701 ccattcccgc aggatggggt gtgggccttc cgctcacgcc ctgacctcga cccacccgtc
     2761 gtcgaggtga tcacagaggc gcacgatgac accgcccccg gctatatctt cgtcgccccg
     2821 gagaaaggcg gcacagggaa gggcggctcg atgatcatcg acgatcgcgg gcaggttgtg
     2881 tggttccatc ccct
//
LOCUS       DATMJA010000107         7357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_74082, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000107 DATMJA010000000
VERSION     DATMJA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74082"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..815)
                     /locus_tag="VNT12_02425"
     CDS             complement(54..815)
                     /locus_tag="VNT12_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45000.1"
                     /translation="MRRINLLPADERGRPADRLRRGVVGILLIVGAFVVIVMVGVYLV
                     LLLRLGDLEDQVAKLDDEIARQNAHLAELSPYRDLKARLEAKKPIADGIYRSRFPWDQ
                     FLQGLAFVVPESTALDTFTAEAAPVDIDAPVKQPLDPPGTITFTGVALPRYENVADFV
                     VRMDNLRYLANADLELATGPSNSRVSPLSFEVASELVTLVGDNGTEVRLEGGSQDELA
                     GRRGSLRTREVANMKRPSYRAAGEQYARHTGHGRR"
     gene            complement(812..1927)
                     /gene="pilM"
                     /locus_tag="VNT12_02430"
     CDS             complement(812..1927)
                     /gene="pilM"
                     /locus_tag="VNT12_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HWI45001.1"
                     /translation="MARLPGFRGPQTVGLDIDRGSIKAVQISGAGGGYALQHVGYHRL
                     PPGAILDGEVADHDLLAAEIREFWDSHSFNGKAVTLGVSNQKVVVRLLDFPHMAQADL
                     QGAISFEAQDHIPMPLDEAVLDYVVLGPREERSDLDRVLVVAAQREMISRYTSAVRTG
                     GLRPAGVDVKALSLTRSTLPDPFFGDEGALLLLDVGAEITNLVVADRGNPVLTRFVPV
                     GLVDFVAAVVELADLPEDEAERLALDPGVRLGSEQDGAQETEAEEGSFDPALVYDVRR
                     GLEGAAQTLADEVQRSIEHHRSQEAAREVSRVLISGEAALISNLDGYLGELLDVPTSR
                     GNPVEKLSANRSNISDDQLRAMEPVLAVAFGLAMEEE"
     gene            complement(2013..2648)
                     /locus_tag="VNT12_02435"
     CDS             complement(2013..2648)
                     /locus_tag="VNT12_02435"
                     /inference="COORDINATES: protein motif:HMM:NF036822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HWI45002.1"
                     /translation="MPEPEVEVQRLAFDEEMRVAIVGAGGYGRVALDVLLAGGLGDRV
                     LGFYDDAHAAISERVRGYPVLGDVEMLKSMLSVEPVHVVVAITDNTARLRVANSLRGL
                     GGQFFTAVHPLSYVSDVATIEGGCVVAAGVAVHPDTAVGSHCFLGSGSVVDRDAEVGA
                     GSWISAGAVIGSGARVGARAFLGPNSSVGRKAYVGADVEVGALERVAREGA"
     gene            complement(2826..3743)
                     /gene="fmt"
                     /locus_tag="VNT12_02440"
     CDS             complement(2826..3743)
                     /gene="fmt"
                     /locus_tag="VNT12_02440"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWI45003.1"
                     /translation="MKIAFAGTPHFAASVLQGLISSPHEVGLVISQPNPRKGRGRRTE
                     RTPVAELAHSQDLLLRQPDSISEISDELSNYDALIIAAYGQILRPETLCAASEGAWNV
                     HASLLPKYRGAAPVERAIMAGETETGVTIIRMDEGLDTGPMALQHPVAIPPEMNAGEL
                     TELLANTGAKAIVEGLNLLQTNTLTLVDQDSRHATYASKISNEDRIIRWGREVGEVHD
                     LIRALAPHIGARTYHRDVEGPIKIWRSTMLDREAVPSEAGHIDAENGRITVYCGKGCL
                     EVLELQVPGGRRLPARDFLLGNSLEGAFS"
     gene            complement(3753..4250)
                     /gene="def"
                     /locus_tag="VNT12_02445"
     CDS             complement(3753..4250)
                     /gene="def"
                     /locus_tag="VNT12_02445"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWI45004.1"
                     /translation="MALDIRKFGDPVLKTRAAPVESFDESLVRLTQDMLATMRDHEGV
                     GLAATQVGRLKRVLVAAVEDDEYVIVNPVLTDRSQTTETATEGCLSIPGIQVDVERPV
                     AVTISGQDVSGRPLQIEASDLLARVLQHEVDHLDGVLILDRTDRQSRKAAMREWREQL
                     LAQQG"
     gene            complement(4251..6497)
                     /locus_tag="VNT12_02450"
     CDS             complement(4251..6497)
                     /locus_tag="VNT12_02450"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45005.1"
                     /translation="MDRAPSRATRKPHAARVSLLIPTHALPPLSYRIPERLGASVRVG
                     TAVVAPLSGRQRLGIVVATEDESDRSRESVVSVVPDLSLQPALVQLCRWIHANAAVPL
                     PAVLRAALPPGIEAGRYRVIEPSPGWPWGVDSSVTRATLKRTLGPDGLRAAQAEGRIV
                     LVPSAPQPATVEWTVIRAAAEPDLTRAPRQRDLFVTLKERGGSSVTSALLSETGASRN
                     SLRELVRRGAARLVRRQEPAPLLPTLCDPGVSERLAPFLRTARRAVKGGGPFLWRTTG
                     RREHAAVAAIVRATLEDGRQALVLAPEVGLVERLVDLLRLVLPEGHTVAPYHSGLGRG
                     RVAVHKAARNGDVDVVVGTRTAALLGLARPGSICVVDEPNGAHRAEQGHEGLPVHVRD
                     VALERARLDRAAVFFLSPFPSLRLYATDVRRREHIRELPTDRSGPWPAVRIVDMRGSG
                     ATFSSNLVNACRRCMEGGGKTGVVLKRLGYATAISCSRCGTVKICPNCDLPLVSQGRE
                     GPLVCTRCGYTEEPGPCVECGSSRMRQTGLGVERVRDELSEVLGVRVGLITAEGQDNA
                     DAPVVAGTAPRILEGEWDAVILPDADAFLAGNGIGAVERAFRLFYSSAEVARKLLLIQ
                     THVPEHYALRAAVRGDYEAFAAAELPRLRALGYPPFAYSASLNFEGSEAALFGAVESR
                     LRPALETGVEISGPVLLGRTGETGAWRLLLRARRRPVVARAATLAARIAARTRGLEVR
                     VDVDPEEV"
     gene            complement(6510..>7357)
                     /gene="metK"
                     /locus_tag="VNT12_02455"
     CDS             complement(6510..>7357)
                     /gene="metK"
                     /locus_tag="VNT12_02455"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWI45006.1"
                     /translation="EARYDEALDEIGAGDQGMMFGYACEETEELMPLPITLAHALTRR
                     LSEVRKGGVLGYLRPDGKSQVTVRYEGDRPVGIEKILISAQHRDGVEAHIRDDLLETV
                     IRPVMPEEFFDPGEAEIMVNPTGRFVTGGPQGDTGLTGRKIIVDTYGGSAPHGGGAFS
                     GKDPTKVDRSASYAARWVAKNLVAAGLAGRCLVQVAYAIGVAHPVSVMVETFGTARTD
                     PATLGSLVQEHFDLRPGAIIRDLKLRNPIFTRTAAYGHFGRSEPGFTWEETSLADDLR
                     RAAGF"
BASE COUNT         1324 a         2387 c         2290 g         1356 t
ORIGIN      
        1 gactatcact attccaagta tttcgaggat gagtatgtta cgatcgttcc gattcatcgg
       61 cgcccatgtc ccgtatgcct agcgtactgt tcgcctgccg ccctgtaaga gggacgcttc
      121 atattggcga cctcgcgggt acgcagcgaa ccccgtctgc ccgcgagctc gtcctgcgat
      181 ccgccttcca gccttacctc cgtgccgtta tccccgacca gcgtgaccag ttccgaggcg
      241 acttcgaagc tcaaagggga gactcgtgag ttcgatggcc cggtggctaa ttcgagatcc
      301 gcgttggcga gataccggag gttgtccatg cgcacgacga agtctgccac attttcgtag
      361 cggggcaacg cgacgcccgt aaacgtgatc gtgcccggtg gatctagagg ctgcttgacc
      421 ggcgcgtcga tgtcgaccgg tgcggcctcg gcggtaaagg tgtcgagggc ggtgctctca
      481 ggcacgacaa aggccaggcc ctgcaagaac tgatcccagg ggaagcggct gcggtagatg
      541 ccgtcggcga tcggcttctt ggcttcgagg cgcgccttga gatccctata cgggctcaac
      601 tcggccaggt gcgcgttctg ccgagcgatc tcatcatcca gctttgcgac ctggtcttcg
      661 agatcaccca acctcagaag cagtaccagg tacacgccga ccatcactat gaccacgaag
      721 gcacccacga tcagaagtat gccgacaacc ccgcggcgta acctatctgc ggggcggccc
      781 cgctcgtcgg caggaaggag gtttattcgc ctcattcttc ctccatggcg agaccaaacg
      841 cgactgcgag tacgggctcc atcgccctca actggtcgtc ggagatgttg gagcggttgg
      901 ctgagagctt ctccacaggg tttccgcggc ttgtcgggac gtccaacagt tccccaagat
      961 acccgtcaag gttagagatc aaagccgctt ctccagagat aaggaccctg gagacctcgc
     1021 gggcggcttc ctgggagcgg tggtgttcga tggaacgctg cacctcgtcg gcgagggtct
     1081 gcgcggcgcc ttctagcccg cgtctcacgt cgtagacgag cgccgggtcg aaactacctt
     1141 cctcagcctc tgtttcctgg gcgccgtcct gctcggaacc caacctaacc cccgggtcga
     1201 gcgccaacct ctcggcttca tcctcaggaa ggtcagcgag ctcgactaca gcggccacga
     1261 agtcgaccag gccgacgggc acaaagcggg taaggacggg attaccccga tccgccacca
     1321 cgaggttggt gatctccgcc ccgacgtcga ggaggagtaa ggctccttca tcgccgaaaa
     1381 agggatcggg gagggtggag cgcgtcaagg agagcgcttt gacatccacg ccggcaggcc
     1441 gcagcccacc ggtccggacc gccgatgtgt aacgtgagat catctccctc tgggcggcga
     1501 ccacaagcac gcggtcgaga tccgagcgct cctccctggg gccgagtacc acgtagtcca
     1561 aaacggcttc atcgagcggc atcggtatgt ggtcctgggc ttcgaagctt atggcgccct
     1621 gcaggtcggc ctgcgccatg tgcggaaagt cgagcagcct gaccactacc ttctggttgg
     1681 agacgccgag ggtgacggcc ttgccgttga acgaatgcga atcccagaac tccctgatct
     1741 cggcggccag gaggtcgtgg tcggcgacct ctccatcgag aatcgctccc ggaggaagcc
     1801 tgtgatagcc gacatgctgc agggcatacc cgccgccggc gccggagatc tggacggcct
     1861 tgatggaccc gcggtctatg tcgaggccga cggtctgcgg ccccctgaag cctggcaaac
     1921 gcgccattac cccgactccc tgcgaaggcg agacgggacg agaacctgtg cagcgaaccg
     1981 gcgactgggc accctacgga tgtccggtga gatcaagcgc cctccctggc gacgcgctcg
     2041 agcgcgccta cctccacgtc agctcctaca taggccttgc gtccgacact ggagttggga
     2101 ccgaggaacg ctcttgcccc gactcgggcc cctgaaccta tgaccgcgcc cgcggagatc
     2161 caactgccgg cccccacctc cgcgtcccta tccaccacgg acccagaacc caagaaacag
     2221 tggctcccca cagccgtatc gggatgcacg gcaacgcccg ccgcgacgac gcaaccaccc
     2281 tcgatcgtgg cgacgtcaga gacgtacgag agcggatgta cagccgtgaa gaactgtcct
     2341 ccaagacccc tcaaggagtt ggcaacccgc aacctggcgg tgttgtccgt gatcgccact
     2401 acaacgtgca caggctcgac cgagagcatg ctcttgagca tctcgacgtc cccaaggacc
     2461 ggataccccc tcaccctctc cgagatcgcc gcgtgggcgt cgtcgtaaaa cccgagcacg
     2521 cgatctccca acccacccgc caacagaacg tccaaagcga cccttccgta gccgccagct
     2581 cctactatgg cgactctcat ctcttcatcg aaggccagcc gctgcacctc gacttcgggt
     2641 tcaggcatgc cggtagtggt ttcgggctct cttatctccc actccctcca acagagatcg
     2701 ctccaagcgc cccaggaagt ttagcatcgt tgttgacagg ttgctcctat acaccacatc
     2761 atcatgcgct cgcccactgc gactatgcta acccactgcg actatgctaa gtataggtgt
     2821 cctggctagc taaacgcacc ttctagtgag ttaccaagca ggaaatcacg cgcagggagt
     2881 cgtcggccac cgggaacctg aagctccagc acctcgaggc aacctttccc acaatacact
     2941 gtgatacgcc cgttttcggc gtctatgtgt cctgcctcgg aaggcacggc ttcacggtcg
     3001 agcattgtgg aacgccagat cttgatcggc ccctcgacgt cgcgatggta cgtcctcgcc
     3061 cctatgtgcg gggccagggc ccttatcagg tcatgcacct caccaacctc ccttccccat
     3121 cttatgatcc tgtcctcatt cgaaatctta gatgcataag tagcatgacg actgtcttgg
     3181 tcgacaaggg tcaaggtatt tgtctggaga aggttcaaac cctctactat agctttagct
     3241 ccggtattgg ccagaagttc ggttagttct cccgcgttca tttcgggtgg tattgccacg
     3301 gggtgctgga gtgccatggg gcctgtatcc aggccttcgt ccatgcggat gatggtcacg
     3361 ccggtctcgg tctccccggc cataatagcg cgttcgacgg gggctgcgcc gcggtatttc
     3421 ggcaggagtg aggcgtggac gttccaggcg ccttcggagg cagcgcagag ggtttcggga
     3481 cgcaggatct gtccgtaggc ggcgatgatt agggcgtcgt aattggagag ttcgtcgctg
     3541 atctcggaga tgctatccgg ttggcgcagg aggaggtctt gggaatgggc gagttcggct
     3601 acgggggttc gctcggtcct acgtcccctg ccttttcgag ggtttggctg ggagatcacg
     3661 agccccacct cgtggggaga ggatatcaga ccctgcagga cgctcgccgc gaagtgtggg
     3721 gtacctgcga aggcaatctt caaggtgtcc tgtcagccct gctgggcgag cagctgctcc
     3781 cgccattcgc gcatggctgc cttgcgtgat tgcctgtccg tgcggtccag tataaggaca
     3841 ccgtcgaggt ggtcgacctc gtgctgcaag accctggcta ggaggtcgga ggcctctatt
     3901 tgcagcggcc tgcctgagac gtcctggcca gagatggtta cggcaacagg acgttcgacg
     3961 tcgacctgga tccctggtat ggagaggcag ccttcggtag cagtctcggt cgtttgcgac
     4021 ctatcagtga gaacggggtt gacgataacg tactcatcgt cttcgacggc ggcgactaac
     4081 acccgcttga ggcgtcctac ctgggtggct gccaggccca ccccttcatg gtcgcgcatg
     4141 gtggcaagca tatcctgggt gaggcggacc agcgactcgt cgaagctctc gaccggcgcg
     4201 gcgcgcgtct tgagcacagg gtctccgaat ttcctgatat ctagtgccaa ctatacctcc
     4261 tcgggatcca cgtcaacgcg aacctcaagc ccacgcgtgc gggctgctat cctggcggcg
     4321 agggtcgccg cgcgggctac gacgggccgc ctcctggccc tgagcagcag ccgccatgcg
     4381 ccagtctctc cggtgcgccc gagtagaaca ggacccgaga tttcgacccc ggtttcgaga
     4441 gccggccgca aacgggattc taccgcaccg aagagcgctg cttcggaacc ctcgaagttc
     4501 agagaagcgg agtacgcgaa cggagggtag ccaagcgcgc ggagccttgg gagctccgcg
     4561 gcggcgaacg cttcatagtc acccctgacc gcagcgcgca gggcgtaatg ctcggggacg
     4621 tgcgtctgga tgaggagcag ctttctggct acctctgccg agctgtagaa taatctgaaa
     4681 gctctctcca cggcaccgat gccgtttccc gcaagaaacg cgtcggcatc gggcaggatc
     4741 accgcatccc actcgccttc cagaatgcga ggtgctgtac ctgccacaac aggagcgtcg
     4801 gcgttgtcct ggccttcagc agtgatgaga ccgacccgca ctccgaggac ctccgagagc
     4861 tcatcacgca cccgctcgac accaaggcct gtctgccgca tgcggctaga accgcactcg
     4921 acacacgggc ccggctcctc tgtgtatccg caacgggtgc agaccagggg accttcccgt
     4981 ccttgcgaga cgagcggcag gtcgcagtta gggcagatct ttaccgttcc gcagcggctg
     5041 catgagattg cggtcgcata accaagccgt ttgaggacga cacccgtttt tccccctcct
     5101 tccatgcagc ggcggcaggc gtttacaagg ttggagctga acgtcgcgcc tgagccgcgc
     5161 atgtctacga tccggacggc aggccacggc ccggatcggt ccgtgggaag ctcgcgtatg
     5221 tgctctcgcc tgcggacgtc ggtggcgtag agcctgagcg atgggaaagg cgagaggaag
     5281 aatacggccg cccggtcgag gcgggcgcgt tccagcgcta cgtcgcgcac gtgcacggga
     5341 aggccctcgt gaccctgctc ggccctgtgt gcgccgttcg gttcatcgac cacgcagatc
     5401 gaaccagggc gtgccagacc gagcagggcc gccgtcctgg tcccgaccac gacgtccacg
     5461 tctccgttgc gggcggcctt gtggacggcg accctgccac ggcccaggcc gctgtggtaa
     5521 ggggctacgg tgtgtccctc cggaagtaca agacgcagga ggtccaccag cctttcgacc
     5581 aaccctacct ccggcgcaag taccagggcc tgtctaccat cctccagcgt cgcccttact
     5641 atggccgcga ccgcggcgtg ctcccgtctc cctgtcgtcc tccacaagaa gggaccgccg
     5701 ccctttacgg ctcgccgcgc ggtccgtaag aacggggcga ggcgttcaga gacgccaggg
     5761 tcacagaggg tgggcagcaa gggggccggc tcctggcggc gcacgagcct tgctgcaccc
     5821 ctgcgcacca gctcccgcag ggagtttcgg ctcgcgccgg tctccgagag cagggcggag
     5881 gtcacgctcg agccgccgcg ctctttcaag gtcacaaaca ggtcgcgctg gcgtggggca
     5941 cgggtcaggt caggttctgc ggcggcacgg atcacggtcc attccaccgt cgccggttga
     6001 ggcgctgacg ggacgagcac gatccgcccc tccgcctggg ccgcgcgcag gccatcaggc
     6061 ccgagggtgc gcttcagggt cgcgcgcgtg acgctactgt caacgcccca tggccagccg
     6121 ggagaaggct ctatcacgcg gtaacgtccc gcttcgatgc caggcggcag ggcggctctc
     6181 aacaccgccg gcaaagggac cgctgcgtta gcgtggatcc agcggcacag ttgtacgagt
     6241 gcgggttgca gcgagagatc aggcacaacg gagactaccg attcgcggga gcggtcactc
     6301 tcgtcttcgg tggcgacgac tatgccgagg cgctgccttc ccgagagcgg cgcgacgact
     6361 gctgttccca cccgaacgct tgcaccaagg cgctcaggta tcctgtagct aaggggaggt
     6421 agggcatgcg tgggtatgag caggcttact cgtgccgcgt gcggctttcg tgtggcgcgg
     6481 gagggcgccc ggtccactct ctttaccaat tagaagccgg cggcgcggcg gagatcatca
     6541 gccagcgagg tctcctccca ggtgaaccct ggctcactgc gtccgaagtg gccgtatgcc
     6601 gcggtccgcg taaagatggg gttgcgcagc ttcaggtcgc ggatgatagc gccaggcctg
     6661 aggtcgaaat gttcctgcac cagcgagccg agggtcgccg ggtcagtcct tgcggtgccg
     6721 aacgtctcga ccatcacgct cacgggatgg gcgacgccga tcgcgtaagc cacctggacc
     6781 aggcaccggc ctgcgagccc tgctgcgacg aggttcttcg ccacccacct ggcggcgtag
     6841 gaggccgacc ggtcgacctt ggtcgggtcc ttgcctgaga aagcgccccc gccgtgcggg
     6901 gccgaacccc cgtaggtatc cacgatgatc ttgcgtcccg tgaggcccgt atcgccttga
     6961 ggtcctcccg taacgaacct gcccgtgggg ttgaccatga tctcggcctc gccggggtcg
     7021 aagaactcct caggcattac agggcgtatg accgtctcga ggagatcgtc cctaatgtgg
     7081 gcctccacac catcgcggtg ctgggccgag atcaggatct tctcgatgcc gacgggtctg
     7141 tccccctcgt agcggacggt aacctggctc ttgccatcgg gcctgaggta gccgaggact
     7201 ccgcctttgc ggacttcgga gaggcggcgg gtgagcgcgt gtgccagtgt gatcgggagc
     7261 ggcatcaact cctccgtctc ctcgcaggca tagccgaaca tcatcccctg gtcgccggcc
     7321 cctatctcgt cgagagcttc gtcgtacctc gcctcga
//
LOCUS       DATMJA010000108         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_74340, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000108 DATMJA010000000
VERSION     DATMJA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74340"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(621..1553)
                     /gene="thiL"
                     /locus_tag="VNT12_02460"
     CDS             complement(621..1553)
                     /gene="thiL"
                     /locus_tag="VNT12_02460"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWI45007.1"
                     /translation="MNEFDVIRRISALLPPAPPEVLVPLGDDCAVLEIGDSTWVAASD
                     MLVSGHQFKDWATPEDVGYKAVAVNVSDVAAMGGTPRFVLASGGAPDPETALRCSQGV
                     IEACESFGVYPLGGDTTSADALTVDVAILGELATQPVLRSGASPGDLLAVTGELGASA
                     AGLLALERGMTGPERLIRRCLRPEPRLGAGRVAARLGATAMIDLSDGLASDVRHLCDR
                     SGVGCRVDLDLLPVKDDVRELARSLERYPEILAATGGEDYELLICAPGPVLDALANCI
                     EVPLTLIGEITRSDVVFERGDEQVEGLSGWNHFT"
     gene            complement(1550..1774)
                     /locus_tag="VNT12_02465"
     CDS             complement(1550..1774)
                     /locus_tag="VNT12_02465"
                     /inference="COORDINATES: protein motif:HMM:NF033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HWI45008.1"
                     /translation="MGIVLFVVAVLFWVAVPAVLLTPLSTEQKAWTSSAFLILGEVAF
                     WVAAVALGREVFRRYRRYLDPRGWLGKKRL"
     gene            complement(1834..>2190)
                     /locus_tag="VNT12_02470"
     CDS             complement(1834..>2190)
                     /locus_tag="VNT12_02470"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI45009.1"
                     /translation="LRRRKPPVGLWENPGGMLEEGEDFEACASRELYEETGVRAQIED
                     AWWARVEPWRNPDDPELYAGVGFLARHQGAEIDPEAAAHDASMWVTETGWRSLRTWYT
                     KRESDMLWKTILEMQR"
BASE COUNT          393 a          702 c          711 g          384 t
ORIGIN      
        1 ccgagaacca cggcggccgg gtcaccgtcc agagcacccc tggtgaaggg tccaccttca
       61 ccgtatatct gccgcgtgcc tagggcgctt tgcttcgcaa aggtttcggc ctgcgaggca
      121 tctatgttgt cgctggcaac gtaccctaga ggcagatgat gggtttgtag cgctggaggt
      181 gctggtggag cctgaggacc tggacgccgt actcgagaga tgggtgcccg cttcttccgc
      241 cgacgttggc acagcgcaca tggaggctcc tctgtcgacc gggaggtact cgcgcgactg
      301 cgcaggctac agggtgaaga agagccggac attgtggccg atctcgccgg catgtttctg
      361 aaggatgcgc gctcgcggct tgtggcggta gaagtggctt tgcggaaggg cgatgcgccc
      421 gccgcggagc gggcggcgca cacgctcaaa ggcggctcgg gcagcatggg cgcaaggagt
      481 atgtccggcc tctgcgcgca gttggaacac gtcggcgcaa caggagatct ctcgcaaggc
      541 tccgaactgc tcggacgaat cagggaggag ttgggtcggg tcgaaagggc gcttgaggca
      601 gaggtttcag gtaacagctt tcaggtaaag tggttccagc cagataagcc ttcgacctgc
      661 tcgtctccac gctcgaagac gacatcggac cgggtgatct ccccgatcaa tgtgaggggg
      721 acttcgatgc aattcgccag tgcgtccagg acgggcccgg gggcacagat caggagctcg
      781 taatcttcgc ctcctgttgc cgcgaggatc tcggggtagc gttcgaggga gcgtgcgagc
      841 tctctgacat cgtctttgac aggcaggaga tccaggtcca ctctgcatcc caccccgctc
      901 cggtcacaca ggtggcgtac atccgaagcc aggccgtcgg ataggtctat catggcggtg
      961 gcgccgagcc tggctgcgac gcgcccggcc ccgagcctcg gttcgggccg caggcacctg
     1021 cgaatgagac gctccgggcc ggtcatgcct ctctcgaggg cgagtagtcc ggccgccgaa
     1081 gcgccgagct cgcccgtgac agccaggagg tcgccggggc tcgctcccga gcgcagtact
     1141 ggctgcgtgg cgagctcccc gaggatggcc acgtccaccg tgagggcgtc cgcgctggta
     1201 gtatcaccac cgagcggata gacgccgaag ctctcgcagg cctcgatgac accctgagag
     1261 cagcgtagcg cggtctcagg gtcgggcgct cctccagagg ccaggacgaa cctcggggtc
     1321 ccccccatcg ccgccacgtc cgagacgttg accgcgacgg ccttgtagcc gacgtcctcg
     1381 ggcgtagccc agtctttgaa ttggtggcca gagacgagca tatcggaggc ggccacccac
     1441 gtactgtccc cgatctctag aacggcgcag tcgtccccga ggggcacgag cacctcgggg
     1501 ggcgcgggag gcaggagggc tgagatcctg cgtataacat cgaactcgtt caaagacgct
     1561 tcttgcccaa ccagccgcgg ggatcgaggt atctcctgta gcgccggaac acctcgcgcc
     1621 caagcgccac ggcagcgacc cagaaggcta cctctcccaa gattagaaag gccgaactcg
     1681 tccaggcctt ctgctcggtg gagagagggg tcagcagtac ggccgggacc gccacccaga
     1741 acagcacagc gaccacgaac aggactattc ccaagagccg cgtcaggcca gccgttttcg
     1801 aatcgcgagg ctcctcaccc gctctttgcc cgtttatcgc tgcatctcca agatagtttt
     1861 ccagagcatg tctgactctc tcttcgtata ccaggtacgc agcgaacgcc acccggtttc
     1921 cgtaacccac atagaggcgt cgtgggccgc cgcctcaggg tctatctctg ctccctgatg
     1981 cctcgccaaa aagcccacgc cggcgtagag ctcagggtca tctgggttgc gccagggctc
     2041 gaccctggcc caccaggcgt cttcgatctg ggcccgcaca cctgtctctt cgtagagctc
     2101 gcggctggcg caggcttcga aatcctcgcc ttcttcgagc atgccgccag ggttctccca
     2161 gagcccaacg ggcggtttgc ggcgcctgag
//
LOCUS       DATMJA010000109         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_370681, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000109 DATMJA010000000
VERSION     DATMJA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370681"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VNT12_02475"
     CDS             complement(<1..313)
                     /locus_tag="VNT12_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWI45010.1"
                     /translation="MSGRADEVIRGVEGVLPGESATARAAHESLKGERRGIRKILPFL
                     GPAFIAAVAYVDPGNFATNISGGAQFGYLLLWVILASNLMAMLIQSMSAKLGIATGKN
                     LP"
     gene            429..1115
                     /locus_tag="VNT12_02480"
     CDS             429..1115
                     /locus_tag="VNT12_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWI45011.1"
                     /translation="MNDLSETRSPSSSVGDYLKAVWELAADAEGVASTKSVATRLSVS
                     PASVTNMFARLQEMGLVRYERYRGATLTERGRKEALRLVRRHRLIETFLLEHLGYDWQ
                     EVHDEAERLEHAVSDGFTGRLAELLGHPDHDPHGDPIPSAEGTLEVDDSFTLNQAVAG
                     QRLRISKVRDEDAAMLDYLGERNLVPGRCLSVREVRALDGVVIVEDEEAEVYALGEPL
                     ARSIFVRNDS"
     gene            complement(1099..1896)
                     /locus_tag="VNT12_02485"
     CDS             complement(1099..1896)
                     /locus_tag="VNT12_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI45012.1"
                     /translation="MKEREEVAVVTGAARGIGRRVALVLAEEGYRIAANDLHKPEVTL
                     EELRSAGAEALSVLGDVSDEAEVREMAGAVMGEFGRLDVLVNNAGISAIVPADEMTLA
                     EWNRTLAVNLTGPFLMCREFGKVMLERGSGSIINVSSVAGLLGVSDRAAYNASKHGLI
                     GLTRTLAAEWGGRGVRVNAVCPGWVKTEMDEEDQASGGYTDADIEGRVPMARFAAPED
                     VARAVAFLADPEWSGFVNGHTLSVDGGWFADASWESLRRRKQATNRS"
     gene            complement(1995..>2399)
                     /locus_tag="VNT12_02490"
     CDS             complement(1995..>2399)
                     /locus_tag="VNT12_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI45013.1"
                     /translation="ATGVRYVELDELLCTCDFISVHTPLTDETHHLIGTTELGRMKPE
                     AVLVNTSRGPVVDEAALADALAEGRIFAAGLDVYENEPEVHPKLLELENVVLAPHIGS
                     ASVETRDRMAVLAAENIVAVLSGEEPKTPVNG"
BASE COUNT          432 a          773 c          735 g          459 t
ORIGIN      
        1 cgggcagatt cttgcccgtg gcgatcccca gcttggccga catagactgg attagcatgg
       61 ccatgagatt ggaggcgagg ataacccaca acagcagata cccgaactgc gccccacccg
      121 agatgttcgt cgcgaagttc ccagggtcca cgtacgcaac cgcagcgata aacgcaggcc
      181 cgagaaaggg caggatcttg cgaatccccc tcctctcacc cttcagcgac tcgtgcgcag
      241 cccgcgcggt cgcactctcc ccaggcagca ctccctccac tcctcttatc acctcgtcag
      301 cccgtccact catgaaaacc tctatcgtct cgaatttagc tttggctaat cttatattga
      361 gttatgatga atagtcaagg cgtcttgctc ttgccaaaag gttcgcatag tctaatatgg
      421 gccatgagat gaacgacctc tcggagactc gttccccttc ttcctcggtc ggggattatc
      481 tgaaggctgt ttgggagctc gctgcagacg cagagggcgt tgcctcgacc aagagtgtgg
      541 cgacgcggct ctcggtctcg ccggcctcgg tgaccaacat gttcgcgagg ctacaggaga
      601 tgggcctcgt caggtacgag cgttacaggg gagcgacgct taccgaacgc ggccgaaaag
      661 aggctttacg gctggtacgg cgacaccgtc tgatcgagac tttcctgttg gagcacctcg
      721 gttacgattg gcaagaggta cacgacgagg cagagcggct tgagcacgct gtttcggacg
      781 ggttcaccgg gcgtctggcg gagttgttgg ggcatccgga tcacgaccct catggagacc
      841 cgatcccctc ggcagaaggc accctggagg tggacgattc ctttacgctg aaccaggccg
      901 tcgcaggcca gcggctgcgc atctccaagg tcagggatga ggatgccgcg atgctcgact
      961 acttgggtga acgcaacctc gtcccaggga gatgcttgag tgtcagggag gtaagggccc
     1021 tggacggtgt tgtaatcgtc gaggacgagg aggcggaggt ctacgcactc ggggagccgc
     1081 tggcgcgttc gatcttcgtc aggaacgatt cgtagcctgc ttgcgccgcc gcaggctctc
     1141 ccaactcgcg tccgcgaacc agccgccgtc cacggaaagg gtatgtccgt tgacgaagcc
     1201 gctccattcc ggatcggcga ggaaggcgac ggccctggca acgtcctccg gagcagcgaa
     1261 tcgggccatc gggacgcggc cctcgatgtc cgcgtcggtg tagccgccac tggcctggtc
     1321 ctcctcgtcc atctccgtct tcacccagcc ggggcataca gcgttgacgc ggacgccgcg
     1381 tcctccccac tcggcggcga gggtgcgggt cagcccgatc agaccgtgct tgctcgcgtt
     1441 gtaagccgcc cggtcggaaa cgccgagcag cccagccacg gagctaacgt tgatgatgct
     1501 ccccgagcct cgctcgagca tcaccttgcc gaactccctg cacatgagga aaggcccggt
     1561 caggttcacg gcgagtgtcc tgttccattc cgcgagcgtc atctcatccg cgggcacgat
     1621 ggcgctgatg ccggcgttgt ttaccaacac gtcgaggcgc ccgaattccc ccatcacagc
     1681 tccggccatc tcacgcacct cagcctcatc cgagacgtcg ccaagtacgg agagcgcctc
     1741 cgctccggcg gagcgcaact cttccaaagt cacctccggt ttgtgtaggt cattggcggc
     1801 gatccggtat ccttcctcgg ccagcacgag ggcgacgcgg cgaccgatac cccgggcggc
     1861 tccggtaaca acagcgacct cttccctttc cttcattcgt cactcctcct atttggcagc
     1921 ccagaggtat gtgagagcgc gactcttgcg ctggccgctc tctaccaggg aacgcaggtg
     1981 cgccacgagg ccagctatcc gtttaccggt gtcttcggct cctcgccgga gagaaccgca
     2041 acgatattct ccgccgcgag gaccgccatc ctgtcgcggg tctcgacgga agcgctgccg
     2101 atatgcgggg cgaggacgac gttctcgagc tccaggagct tggggtgtac ctcgggctca
     2161 ttctcgtaca cgtcgaggcc cgcggcgaag atacgcccct cggcgagggc gtcggcaagt
     2221 gcagcttcgt ccacgaccgg gccgcgcgag gtgtttacga ggacggcttc tggcttcatc
     2281 cttcccagct ccgtcgtgcc gatgaggtgg tgggtctcgt cggtgagcgg ggtgtgcacg
     2341 gagatgaagt cgcacgtgca cagcagctcg tcgagctcga cgtaccgcac cccggtcgc
//
LOCUS       DATMJA010000110         3200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_726218, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000110 DATMJA010000000
VERSION     DATMJA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726218"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /gene="cofE"
                     /locus_tag="VNT12_02495"
     CDS             <1..441
                     /gene="cofE"
                     /locus_tag="VNT12_02495"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HWI45014.1"
                     /translation="HHGFVCANAGVDASNVPGSDTVCLLPVDPDASATRLREALAEDP
                     GVEVAVVISDSFGRAWRHGITDVAIGVAGMDPVADYRGQRDPHGYPMEASVLAVADEL
                     AAAAELVMGKTDGIPLAIVRGYSYSPASGSARELLMPPERDMFR"
     gene            733..1170
                     /locus_tag="VNT12_02500"
     CDS             733..1170
                     /locus_tag="VNT12_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45015.1"
                     /translation="MRLSVIAGCILMGCGFALEDVIPEFIAVLAAQAVWGVGYTFISG
                     ALEASIADEAPDRDLGRVYLRGEQADYIGSFIGLPASVLLGLVALNLPLIIGGALTIA
                     LGLALVLTMRSRPKRVLLAVKEPNSSNSDEIYRIFVDVWLFGA"
     gene            1320..2078
                     /locus_tag="VNT12_02505"
     CDS             1320..2078
                     /locus_tag="VNT12_02505"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWI45016.1"
                     /translation="MHESLRSKHNDRILPGLKALERESELLEGADAREVIAWAVETYG
                     DGLALSASFGGGEGMALLDMISKVTDKVTVLTIDTGFIFKETAEFREEVMRRYKLPVE
                     VLKPELTLEEQVERYGEQMRTCTPNLCCQIRKIEPLQKALNRYDAWMTGIRREQTPQR
                     TGTKVVAWEERYQAAKIAPMPDWTEAQVWDYVRAHDVPVNPLLHQGYKSIGCEPQTRP
                     VHHDEDPRAGRWAGTDKTECGIHVEGGQISRAGA"
     gene            2119..>3200
                     /locus_tag="VNT12_02510"
     CDS             2119..>3200
                     /locus_tag="VNT12_02510"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWI45017.1"
                     /translation="MRFLREDQTLNKVEKIKQERHPLEVYGAIIDTYSKDLSAMDEVP
                     GEVERLKWVGLYPQKQGGDAFMMRIKIPGGVLTPAQTRVVGKIAADFARGPVPNPHFG
                     NNFLDLTTRQDVQMHWIKMENVPEIWRRLEEAGITTVQACGDSARNVLCCPVSGLGHD
                     EAIDAYPVAQAISDYFTGNREYANLPRKFKMSVTGCLEDCAQAEINDVGMLPARLEDG
                     TLGFNLRVGGGLSDGPRMASDIDVFVRPEEAVEITRGIAQVFGELGDRENRWTARMRY
                     LVQELGPEGFREELAKRVSVGLTPAGEDLTKRYRGDHIGVHAQKPGGGSCKGGSGTAL
                     YYVGLNVPVGRMSGEQFEEAGRLAGE"
BASE COUNT          609 a          959 c         1041 g          591 t
ORIGIN      
        1 caccacggtt tcgtctgcgc caacgcgggc gtagacgcct cgaacgttcc tggcagcgac
       61 accgtgtgcc tgcttcccgt agaccccgat gcctccgcga cgaggctccg tgaggcgctc
      121 gccgaagacc caggcgtcga ggtggccgtg gtgatctcgg actcgttcgg gcgggcctgg
      181 cgccacggta taacggacgt cgctatcggt gtcgcaggca tggaccccgt cgctgactac
      241 agggggcaga gagacccgca cggctaccct atggaggcga gcgtgctcgc cgtggccgac
      301 gaactcgccg ccgccgccga gctggtaatg ggcaagaccg acggtatccc tctggccatc
      361 gtgcgggggt attcgtactc gcccgcttcg ggcagcgcca gagagctcct catgccgcct
      421 gagagggaca tgtttcgcta atttagcatt tcagcacgct ccggcctgcg gccttcgctt
      481 gtcagcactt cagcatttca gcttgctgtc ttggctcgtg gccgatcgct cgtagccgga
      541 tgccggggac ggtatctctg cagattcctc ttgactggcg gcgtttcttg acgccgtctt
      601 aggttctacg gtacgatcgg gccgtgctaa cgcccctcta gacggcggag acaggccagc
      661 aggaccgcaa ggatcgcggt tcccactccg ccgtacccct gcagcgttcc catccgaagg
      721 ccgtcgcgtg ccttgcgact gtcggtcatt gcgggatgca tactgatggg gtgtggtttc
      781 gccttagagg atgtcatccc cgaattcata gccgtgctcg ccgcccaggc ggtttggggc
      841 gtcggttaca cattcatcag cggggcgctg gaggcctcga tcgcggacga agcccccgac
      901 cgagacctgg gaagggtata cctccgcgga gagcaggcgg actacatcgg ctccttcata
      961 ggccttcccg caagcgtgct tctcggtctt gtcgcgctga atctaccgct catcatcggc
     1021 ggcgccctca ccatcgcact aggcctcgcg ctcgtcctca cgatgcgtag caggccaaag
     1081 agggtccttc tcgccgtaaa ggagccaaat tcttctaatt ccgatgaaat ttatcggatt
     1141 tttgttgacg tatggttgtt cggcgcttag tatccggcgc atggagttac acggtacgca
     1201 gggtcatgcg aaggggcgaa tgtctctggc ccctcgcgcg ggcacgcggc gatgtagcgt
     1261 ttgcgcctga tctcaaccct tgactttcta ttacacgatt tacactggag gatcgcgcga
     1321 tgcacgaatc cctgcgttcg aagcataacg acagaatatt acccggtctc aaggcgctcg
     1381 agcgggagtc cgagctactg gagggcgccg acgcgcggga ggttatcgcg tgggctgtgg
     1441 agacttacgg tgatggcctg gccctctcgg cttcgttcgg cgggggcgag gggatggcgc
     1501 tgttggacat gatctccaag gtcaccgaca aggtcaccgt actcaccatc gacacgggtt
     1561 ttatcttcaa ggagaccgcc gagtttcgcg aggaggtcat gcgacgctac aagctgcctg
     1621 tcgaggtctt gaagcccgag ctcacgctcg aggagcaggt ggagcgctac ggcgagcaga
     1681 tgcgcacctg caccccgaac ctgtgctgcc agatccgcaa gatcgaaccc cttcaaaagg
     1741 cgctgaaccg ttacgacgca tggatgacag gcattcgcag ggagcagacc ccgcagagga
     1801 caggcacgaa ggtagtcgcc tgggaggagc gctaccaggc cgccaagata gcgcccatgc
     1861 ctgactggac ggaagctcag gtgtgggact acgtgagggc gcacgacgtg cccgtcaacc
     1921 cgctcctgca ccagggctac aagagcatcg gctgcgagcc gcagacgagg cccgtgcacc
     1981 acgacgagga tcccagggcc ggccggtggg ccggaaccga caagacggag tgcggcatcc
     2041 acgtcgaagg cggccagatc agccgcgcgg gcgcctgaat ttcagagatc ctctctccaa
     2101 aagcgtaggt tctacaacat gagattcctc agggaagacc agaccttgaa caaggtcgag
     2161 aagataaagc aggagcgcca tcccctagag gtgtatgggg ccataataga cacatactcg
     2221 aaggacctct ctgccatgga cgaagtccct ggcgaggtcg agcggttgaa gtgggtcggt
     2281 ttatacccgc agaagcaagg cggcgacgcg ttcatgatgc ggatcaaaat ccctggcggc
     2341 gtcctgacgc ccgcgcagac cagggtcgta ggtaagatag cggccgactt cgcgcggggt
     2401 cctgtgccca acccgcactt cggcaacaac ttcctcgatc tgaccacgcg ccaggacgtg
     2461 cagatgcact ggatcaagat ggagaacgtg cctgagatct ggcgccgcct ggaagaggct
     2521 ggcatcacga ccgtgcaggc ctgcggcgac tcggcccgca acgttttgtg ttgtcccgtt
     2581 tcaggcctcg gccacgatga ggccatagac gcctaccccg tggcgcaggc tatctccgac
     2641 tactttaccg gtaaccgcga gtacgcgaac ctgccgagga agttcaagat gagcgtcacg
     2701 ggatgcctgg aggactgcgc gcaggctgag atcaacgacg tcggcatgct ccctgcccgc
     2761 ctggaagacg gtacgctggg cttcaatttg cgtgtgggcg gcggcctctc cgacggccca
     2821 aggatggcct cggatatcga cgtcttcgtc aggcccgagg aggcggtcga gatcactcgc
     2881 ggcatagccc aggtcttcgg cgagcttggg gacagggaga accgctggac cgcgagaatg
     2941 cgctacttgg tccaggagct cggtcccgag ggctttcgcg aagagcttgc gaagcgcgtt
     3001 tccgtcgggc tcacgcccgc aggagaggac ctgacgaaga ggtacagggg ggatcacata
     3061 ggggtccacg cccagaaacc tgggggcggt tcgtgtaagg gcggttctgg aaccgccctc
     3121 tactacgtcg gcctgaacgt gcctgtgggg aggatgagtg gtgagcagtt cgaggaggcg
     3181 ggtaggcttg caggggagta
//
LOCUS       DATMJA010000111         6366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_15411, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000111 DATMJA010000000
VERSION     DATMJA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15411"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VNT12_02515"
     CDS             complement(<1..267)
                     /locus_tag="VNT12_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWI45018.1"
                     /translation="MEPTETDTPKPVIEPIRRVKLYEEVASRTRDLIVSGELKPGQAL
                     PSERKLAEQFKVGRAVIREAIRRLEVSGLVESRHGGGNYVREVTV"
     gene            1049..2554
                     /locus_tag="VNT12_02520"
     CDS             1049..2554
                     /locus_tag="VNT12_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWI45019.1"
                     /translation="MDTSRGGAPGGTTEEVQEEKVWHAGREDPMPIRELPEPPASLHI
                     LGPGMLLAALGVGLGETYLWPRLVILFGPEIRWLFFIGVTLQGVVMLEMARYATATGE
                     SIFFGAARLWRPIMWLFFAIAVMVYIWPGHVALGAQAFESLTGISWYIFGVIGLLLIG
                     LIISFSTIVYNVVEAILSTLIGVLVIGSSIIAALVGNLGFVWATITGLFTFGYFPQEA
                     ISGPQAAALFPLIVGAIAFAGPSGMQQMWYTLWLRDKGAGMGSYIPRIKGLTHMDEEE
                     SMPSRGFMFDTENPENMRRWKGWRRWVHFDAWVLFWGITMLTTIIYTVLAQSALQIDP
                     GIKAAILADDQGATLNGMSAAFAQAGGAIMGGLFFVFIAVIGWKMSFGIFDAFSRGQA
                     DMSYYFIRPVQRFGMNRLYFVFLWFLILFGIAMLFVTGEEDGPAAILDILSVLSTFAM
                     GAYCLLLLGTNNLLLPKKIRPNIIINILLAFAALFYLGMLAYSYFRFGAIL"
     gene            2604..3041
                     /locus_tag="VNT12_02525"
     CDS             2604..3041
                     /locus_tag="VNT12_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45020.1"
                     /translation="MVIGFVAGLLVGGLAAVASSPTGFIIVAALGLGIPLAIFGIIYD
                     ALLDAGKLSFGYAAPTALYGILTFPIARLVQELLLTAIFGQGITLQQEANVLQFLVFQ
                     GIMGFGYGIGFLMIHNQIIEMSAWRQYRKHVKEEQHQAAEKQA"
     gene            3155..3451
                     /locus_tag="VNT12_02530"
     CDS             3155..3451
                     /locus_tag="VNT12_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HWI45021.1"
                     /translation="MELTVTGEGAGVMEEYVAERVKRLKRMFASEPDISVRFDTDGGK
                     VVAEVVVSIRGYRTLKACSKGDDARGALDKAFKRLEWKYKLFWARGSFRFGDCC"
     gene            complement(3531..4715)
                     /locus_tag="VNT12_02535"
     CDS             complement(3531..4715)
                     /locus_tag="VNT12_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWI45022.1"
                     /translation="MEAPGRALKLDPIKSIKKAPAEEYYQSGHRTCQGCLSALPMRLM
                     AKAAGPRTIVTGTTGCMYVANTTYMSTPWVVPWMHTQLGAAGSSAVGTAAGLKALMRK
                     GKIKDEPINVIAFCGDGGGADMGLAAISAALTDVEYNLLIFLYDNESYANTDIQTSGQ
                     TPWGAVTTFSPPGKKHRIMQRRWKKNVPGMLAAGHPDTKYIASGTPAIPVDMMDKVRA
                     ALSAGGPTYMHVLDPCPKGWHYDPEHSNELGKLAIETGIYPLYEIKEGVCKLTGVTKQ
                     IAEGKRKRKPVREYLEKQGRFAQFKNQDYAYFQKKVDEMWEQWVIPTVVAFSYGGPIN
                     ITGDIPKVRNRNVVRENASWAGIPGHAHEEYKEVVADPYEPWRDDNELGEYPWALPST
                     GQ"
     gene            complement(4778..6004)
                     /locus_tag="VNT12_02540"
     CDS             complement(4778..6004)
                     /locus_tag="VNT12_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HWI45023.1"
                     /translation="MTTQDTTGRREARQEEKLMTGTEAVAQAVRLADVEVTAAYPIRP
                     YDGVMQAVAKLIASGELDAEFIVAEGEHSQFEICKHASSVGARVFVGSSGVGWMYAME
                     SIAVTPSLRLPVVAMIGNRALDDPGAFGVEHNDALSVRDLGWQLVWVEDAQECFDATL
                     MAYRIGEDSRVSFPVGLGVDGAFITHSQALLQIPDQESVDAFLPPFDLGDRVLHPDNP
                     ISIAPQANEDWVMEIRKQADEAAKRVKDVIWEAHTDFKELFGRGGDNPFFEEYMTEDA
                     EYVLFGMGSLGLPAKVMVRRLREQGEKVGFIRLKWFRPFPDEELVEVLGRFKAVGVVD
                     RDYAYGSPQQGGVLFTDLRAALYAAERRPRMINFIGGLGGRELTPEMMDEMADITKRA
                     SEGEEVPVVSWIGVRG"
     gene            complement(6021..>6366)
                     /locus_tag="VNT12_02545"
     CDS             complement(6021..>6366)
                     /locus_tag="VNT12_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HWI45024.1"
                     /translation="SSDRTMRPVVNFDTCIKCRQCWIDCPDECFDISAEELHPVNYSY
                     CTGCGICANVCPVEDCIVMVNDLEFVEEGDKDVFPMWKEDKDGYHEWRDEKIARGRID
                     HDNFFNVDTWAK"
BASE COUNT         1077 a         1967 c         1955 g         1367 t
ORIGIN      
        1 gacggtgacc tccctgacgt agttgccacc accgtggcgg ctctccacga ggccggatac
       61 ctcgagccgg cggatcgcct cccgtatcac cgcacgtccc accttgaatt gctccgcaag
      121 cttcctctcc gaaggcaacg cctgcccagg cttgagctcg ccgctgacta tgaggtcgcg
      181 ggtacgggac gccacctcct cgtagagttt gacgcggcgg atcggctcga ttacaggctt
      241 tggcgtgtca gtttccgtcg gttccactcg attcagttta gcatctctgc acccgagcca
      301 gtctcaagcg ttcggccccc taccgcggta gggggccgaa atcgtcagcg ctgtgcaagc
      361 ttacttgtag tgcttccggt cggtgagtct cggtccactg ctcctgccag gcgagccctt
      421 gcccgacgca cgaaagccct gcgcgctcgg tgccaatgcg cttatcaact tcttgatcct
      481 atgcatcaag gtccagtcct ctcttcctct cttgagtcgg ctcgttcctc gtaatcaacg
      541 tgcgccctat gcgcacaacg ttacgagcct ccgtcaattc cgtctttcaa actggtatgt
      601 tcagttcttg atcttgattg taactaccgc gtatcaagat tcaagcaagc ggtgccaatg
      661 gatgagaaag gtatcacaaa attaggttcg ggaccttaca ccgcactgtg gcgaggggac
      721 aaccgatctc agtaacgatc ttacgaagat atgcgggcgt ttcgcgcggg acaatggtct
      781 gcttgacaca aatcgggcct ctgtgtatgc tggtatgacc tgtcgcaaga gggaaagggc
      841 agataggtcg gctaacaaaa tcgggttagg atgctctgca ttgacgctcg ttttccacgg
      901 ctctgggtaa ggcatttgac ggtcgcggac gcttgctgtt cggggccaca cgcggcgcgg
      961 cgtgattctg accattcgat cgaagtctgt gacggtcgat cgcaggcgca ttgtgcacta
     1021 acctgtcggg gcaagaaagg agattgtggt ggacacgtct aggggtggtg cgcccggggg
     1081 aactaccgag gaagtacagg aagagaaggt gtggcacgcg ggcagggaag acccgatgcc
     1141 gatcagggag ttgcctgagc ccccggcctc gttgcacatc ctcgggcctg gcatgctcct
     1201 ggcggctctg ggggtaggct tgggcgagac gtatctatgg ccgcggctcg tcatactctt
     1261 cgggcctgag atccgctggc tcttcttcat tggtgtcacg ctccagggag tcgtcatgct
     1321 ggagatggcg cgctacgcga cggccacggg cgagagcatc ttcttcgggg cggcgcgtct
     1381 gtggcgtccg ataatgtggc tattcttcgc tatagcggtg atggtctaca tctggcctgg
     1441 ccacgtggcg ctcggggcgc aggcgttcga gagcctgacg gggataagct ggtacatatt
     1501 tggcgtaatc ggcctgctcc tcatcggcct cataatcagc ttctcgacga tcgtctacaa
     1561 cgtggtggag gcgatcttat ccactttgat cggcgtactc gtgatcggct cctcgatcat
     1621 cgcggcgctg gtgggaaact tagggttcgt gtgggctacg attacgggcc tgttcacttt
     1681 cgggtatttc ccgcaggaag cgattagtgg tccgcaggct gccgcgctat tcccgctgat
     1741 cgtaggggcc atcgccttcg ccggtccttc tgggatgcag cagatgtggt acacgctctg
     1801 gctgcgggac aaaggtgccg ggatgggatc gtacatcccc aggatcaagg gcctgaccca
     1861 catggacgaa gaggagagca tgccctcccg gggcttcatg ttcgacacgg agaatccgga
     1921 gaacatgcgg cgctggaaag gctggcggcg ctgggtgcac ttcgacgcct gggtgctgtt
     1981 ctggggcata acgatgctta ccaccatcat ctacaccgtg ctcgcgcaga gcgcgctgca
     2041 gatcgatcct ggcatcaaag ccgccatcct ggccgacgat cagggggcca ccctcaacgg
     2101 catgtccgcc gctttcgccc aggcgggcgg tgctatcatg ggcggtctgt tcttcgtctt
     2161 catagcggtt atcggctgga agatgtcctt cggcatcttc gatgccttct cccgcggcca
     2221 ggcggacatg tcgtactatt tcatccggcc cgtgcagcgt ttcgggatga atagactcta
     2281 cttcgtgttc ctgtggttcc tgatcctgtt cgggatcgcc atgctgttcg ttacgggtga
     2341 ggaggacggc cccgccgcca tcctcgacat cctctcggtg ctctcgacct tcgcgatggg
     2401 agcgtactgc ctgctgctgt tggggaccaa caacctgctt ttgccgaaga agatacggcc
     2461 gaacataatc atcaacatct tgctggcctt cgcagcgttg ttctacctcg ggatgctggc
     2521 ctacagctac ttccgcttcg gggccatcct gtaggattga ggcgtagccg gtgggcgttt
     2581 tgcgacgagt aaagataccg gggatggtga taggcttcgt cgccggacta ctggtggggg
     2641 ggctggccgc tgtagccagc tcccccaccg gctttatcat cgtcgcggcc ctcggactcg
     2701 gcatccccct ggcgatcttc ggcataatct acgacgcgct cctggacgcg ggcaagctca
     2761 gcttcggcta cgcagccccg accgcgctct atgggatcct gaccttcccg atagcgcgtc
     2821 tggtgcagga gctcctgctg acggcgatct tcgggcaggg aatcaccctg caacaggaag
     2881 cgaacgtcct gcagttcctg gtattccagg ggatcatggg cttcggctac ggcatcggct
     2941 ttttgatgat ccacaaccag atcatcgaga tgagtgcctg gcgccagtac cggaagcacg
     3001 tcaaagaaga acagcatcag gccgccgaga agcaagcata acctcccaag cataacctcc
     3061 cgctgcgctt ctacagtgcc gtttgctggc ggacattctg gctgtggtac gatgtatcgg
     3121 atgttccaga tccggtaaag gggcgatgga gcgcatggag cttacggtaa ccggtgaggg
     3181 cgcaggtgtc atggaggagt acgtcgccga acgggtgaaa cgtctcaagc ggatgttcgc
     3241 ctccgaaccc gacatcagcg tgcgcttcga caccgacggc gggaaggttg tcgccgaggt
     3301 ggtagtttcg atccggggct accgcactct aaaggcgtgc agcaaagggg acgacgcacg
     3361 cggtgccctg gacaaagcat tcaagaggct ggagtggaag tacaagctgt tctgggcgag
     3421 gggcagcttc cgtttcgggg actgttgcta gttccttaag ttctgaagaa cgaaaagcgg
     3481 gcgggaactt cccgcccgct taccggctct gtgtgctgcc taaaaccttg ctactgtccc
     3541 gttgaaggca gcgcccaggg gtactcaccc agctcgttgt cgtcgcgcca gggctcgtag
     3601 gggtcggcga caacctcctt gtactcctcg tgggcgtgac ccggaatacc ggcccaggat
     3661 gcgttctccc gcaccacgtt gcggttgcgg accttcggta tgtcgcccgt gatgttgatc
     3721 gggccgccgt agctgaaggc gacgacggtg gggatgaccc actgctccca catctcgtcg
     3781 accttcttct ggaagtaggc atagtcctgg ttcttgaatt gggcgaagcg gccctgcttc
     3841 tccaggtact ccctaacggg cttgcgcttg cgcttgccct cggcgatctg cttcgtcacg
     3901 ccggtaagct tgcagacgcc ctccttgatc tcgtagagcg gatagatgcc cgtctcgatg
     3961 gccagtttgc cgagctcgtt ggagtgctcg gggtcgtagt gccagccctt cgggcagggg
     4021 tcgagcacgt gcatgtaggt cggcccacct gcgctgaggg ccgcgcgcac cttgtccatc
     4081 atgtccacgg ggatggctgg ggtgcccgag gcgatgtact tcgtgtcggg gtggccggcg
     4141 gccagcatgc cagggacgtt cttcttccac cggcgctgca tgatgcgatg cttcttgcca
     4201 ggtgggctga acgtcgtcac ggccccccac ggcgtctggc ccgaggtctg gatgtcggta
     4261 ttcgcgtagc tctcgttatc gtagaggaag atcaggaggt tgtactcgac gtcggtgagc
     4321 gccgcgctga tggcagccag gcccatgtcg gcgccgccgc catcaccgca gaaggcgatc
     4381 acgttgatcg gctcgtcctt gatcttgccc ttgcgcatga gggccttgag cccggcggcc
     4441 gtgcctactg cagacgaccc cgcggcgccg agctgggtgt gcatccacgg cacgacccac
     4501 ggggtgctca tgtaggtggt gttggccacg tacatgcagc ccgtcgtgcc ggtcacgatg
     4561 gtacgcgggc cggcggcctt cgccatgagc ctcatgggca gggcggacag acaaccctgg
     4621 caggtgcgat ggccggactg gtagtactcc tccgccggcg ccttttttat cgatttgatc
     4681 ggatcgagct tcagcgcccg tcctggtgcc tccataacca cgtctcttct atctccttcc
     4741 ttcgttcttt tgcttctctg tacttgttta ctcttcctta cccgcgcacg ccgatccagc
     4801 taacgacagg cacttcctcg ccctcgctgg ctcgcttggt gatgtccgcc atctcgtcca
     4861 tcatctcggg ggtaagctcg cgaccgccca atcccccgat gaagttgatc atgcgaggcc
     4921 gccgctcggc ggcgtagagg gccgcccgca gatcggtgaa caggaccccg ccctgctgtg
     4981 gcgagccgta ggcgtagtcg cgatccacga cgccgacggc cttgaagcga cccaggacct
     5041 ccaccagctc ctcgtccggg aagggcctga accacttcag gcggatgaag ccgaccttct
     5101 caccctgctc ccgcagccgg cgcaccatga ctttggccgg caggccgagc gagcccatgc
     5161 cgaagagcac gtactcggcg tcctcggtca tgtactcctc gaagaatggg ttgtctccgc
     5221 cacgtccgaa gagctccttg aagtcggtgt gggcctccca gatcacgtcc ttgacccttt
     5281 tggcggcctc gtcggcctgc ttgcggatct ccatgaccca gtcctcgttg gcctgggggg
     5341 ctatgctgat cgggttgtcc gggtgtagca cccggtcgcc cagatcaaat ggcggcagga
     5401 acgcgtcgac gctctcctgg tccgggatct ggagcagcgc ctgcgagtgc gtgatgaacg
     5461 cgccgtcaac gcctagtcct acggggaagg agaccctgct gtcctcaccg atgcggtagg
     5521 ccatgagcgt cgcgtcgaag cactcctgcg cgtcctcgac ccatacgagt tgccagccca
     5581 gatcgcgcac actcaacgcg tcgttgtgct cgaccccgaa cgctcccgga tcgtcgaggg
     5641 cgcggttgcc gatcatcgct acgaccggca gccgcagcga cggtgtgacc gcgatggact
     5701 ccattgcgta catccagccc acgcccgagg agcccacgaa cacgcgggcg ccgacggacg
     5761 aggcgtgctt gcagatctcg aactgggagt gctcgccctc cgccacgata aactcggcgt
     5821 ccagctcgcc gctggcgatc agcttcgcca cagcctgcat gaccccgtcg taggggcgga
     5881 tcgggtacgc agccgtcacc tcgacgtccg ccaggcggac cgcctgggca accgcctccg
     5941 tgcccgtcat cagcttttct tcctgccgtg cctcccgacg cccggtcgta tcctgggtgg
     6001 tcatcgcgcc tcccctcctc tcacttcgcc caggtgtcga cgttgaagaa gttgtcgtgg
     6061 tctatcctcc cacgcgctat cttctcgtcc cgccactcgt ggtagccatc tttgtcctct
     6121 ttccacatcg ggaagacatc tttgtcgccc tcctcgacaa actcgaggtc gttgaccatc
     6181 actatgcagt cctcgaccgg gcacacgttg gcgcagatgc cgcatccggt acagtagctg
     6241 tagttgaccg ggtgcagctc ttccgcggag atatcgaagc actcgtcggg gcagtcgatc
     6301 cagcactgtc ggcacttgat gcacgtgtcg aagttgacaa ccggccgcat ggtcctgtca
     6361 gaggag
//
LOCUS       DATMJA010000112         7397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_370800, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000112 DATMJA010000000
VERSION     DATMJA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370800"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..429
                     /locus_tag="VNT12_02550"
     CDS             88..429
                     /locus_tag="VNT12_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45025.1"
                     /translation="MEGDVKRLSVPDEPTELKAGIRALLEDSPEEGGRVIADAAFVCD
                     LLWEEWRADLEGAGMGYDRFLEISRSYAGEFRFWVVGERLWDHCAAGLAGRLLRRLPA
                     GQATNLAEVNG"
     gene            426..1619
                     /gene="sat"
                     /locus_tag="VNT12_02555"
     CDS             426..1619
                     /gene="sat"
                     /locus_tag="VNT12_02555"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HWI45026.1"
                     /translation="MNVSTEYTTIAPHGGELVDRRAPEEQREELLSRAQGFARVTLGP
                     RALSDLEMISTGVFSPLPGFMVSEDYESVVETMHLKDGLAWSLPITLPASEEEANGIR
                     VGEEISLSDEAGRVLATMIVEDRYSYDKEHEAGEVYGTTSPEHPGVAAVYRQKDVLLG
                     GEVTLLVDDPNPKPFPTFHYEPRRLREVFAEKGWKRVVGFQTRNPVHRSHEYIQKSAL
                     EIVDGLLLNPLVGETRAADIPADVRMRSYETILERYYPKDRTMLAVFPAAMRYGGPRE
                     AIFHAICRKNYGCTHFIVGRDHAGPGKDSSGRPFYGEYDAQHIFDEFEEGEIGLTPLF
                     FEHAFFCLQCQGMGTTKTCPHDKVNHVFISGTKVREMLSKGEYPPPEFSRPEVIEVLI
                     EGQRT"
     gene            1684..2256
                     /gene="cysC"
                     /locus_tag="VNT12_02560"
     CDS             1684..2256
                     /gene="cysC"
                     /locus_tag="VNT12_02560"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWI45027.1"
                     /translation="MENGTRFGRGFTLWFTGLSGAGKTTISEIVEHELRNRFRKIEVL
                     DGDIVRTNLSKGLGFSREDRNTNVLRIGFVADLLTRNGVGVIVSAISPFKEVRDQVRR
                     NIGGDFIEIFVDAPLEVCAERDVKGLYEKAFSGEIPQFTGVSDPYEAPTAPELHIKTD
                     EEEPRESAARVIGHLEEFGYLRPVEAFDRA"
     gene            2263..3066
                     /locus_tag="VNT12_02565"
     CDS             2263..3066
                     /locus_tag="VNT12_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI45028.1"
                     /translation="MIDPTPALFGLLVGFLVGLTGVGGGSLMTPFLVAVMGVPAPTAV
                     GTDLTFATFTKLTGSFQHFRQRSVNVEIAVFLGMGSIPAGLLGVGTLEWIKGSFDPAR
                     VESIMITIIAATLVLVGVSLIYRDYFMPKRHEGPKPGRWDGKSRMSRRRRAYTVLFGA
                     LGGYLVGLTSIGSGSVMAVILLLLYPIAPAVIVGTDITHAMVLSFVVGIAHMTQGNVD
                     FALAGTLLLGSIPGVLIGSRMAPWIPGKPLKLILAIMLIFVGARLLLAG"
     gene            complement(3146..3853)
                     /locus_tag="VNT12_02570"
     CDS             complement(3146..3853)
                     /locus_tag="VNT12_02570"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWI45029.1"
                     /translation="MQLENKVAAVTGSSLGIGRAIALAFGREGASVAINYRSHPEEGQ
                     EAVQEIEKGGGKAISVQADVSEPEGVKRLIQQTVKEFGRLDVMVNNAGIEQKIPFLET
                     PLEVWEKVISVNLTGVWLGCQEAASQMVSQGDGGRIINVSSVHEDLPMPTNSPYCAAK
                     GGVRMLMRTIAVELAPHGITVNNIAPGAIETPININLDEHPEQRHELLSEIPLGRIGR
                     PEDVASLAVYLASDASS"
     gene            complement(3837..5216)
                     /locus_tag="VNT12_02575"
     CDS             complement(3837..5216)
                     /locus_tag="VNT12_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWI45030.1"
                     /translation="MRCAVAGPDALWLRASGPHEEVGGEILSRFTVSEGERVPLVLTW
                     SPSYAERPLAINGLQAVDDTERFWLDWAGGISYEGRFQDAVYRSLLTLKGLTYEPSGG
                     IVAAATTSLPETLGGSRNWDYRYCWLRDSTFTLGALIGAGLLEQASCWQQWVLRAVAG
                     DPADTQIMYGIDGARRLPEYELPWLPGYEGASPVRIGNDAAGQVQGDVPGEVLAAAHV
                     GRTAGIVSMERGWELQRWLAGRLQVAWKRPDNGIWESRGERQHFVYSRVMCWVAFDSL
                     VKGVERFGLDGPVELWRAVRDEIHSDILENGYDAGRGTFTQAYGSSALDACALLIPRV
                     GFLPYDDERVVSTIETIRNKLTEDGLVIRYRTDQSDDGLTGREGSFLICSFWMVYALW
                     GIGRHREAEELFERLLALRNDVGLLSEEYDTRAQRLVGNFPQAFTHLALVNCALRLSD
                     REVSIAARE"
     gene            complement(5143..5619)
                     /locus_tag="VNT12_02580"
     CDS             complement(5143..5619)
                     /locus_tag="VNT12_02580"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HWI45031.1"
                     /translation="MPGRIEDYAMVGDLQTAALVGSDGSVDWLCFPRFDSPACFAALL
                     GEDTNGRWRIAPASGGRCTRHRYRGHTLVLETEWETPGGAVRVVDCMPPRGVAPDLVR
                     VVEGVRGKVSVTSELRIRFDYGHVAPWVTLQGREVRRSGPGRAVVARLRAPRGGGR"
     gene            5966..6193
                     /locus_tag="VNT12_02585"
     CDS             5966..6193
                     /locus_tag="VNT12_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45032.1"
                     /translation="MVGNGTVPTFGLCYDFRQKLPLGHYARFYAECLEASYYHLCFWG
                     RLPGVTHEQALANMRLFASDVIPRVRDAVKI"
     gene            6190..7254
                     /locus_tag="VNT12_02590"
     CDS             6190..7254
                     /locus_tag="VNT12_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HWI45033.1"
                     /translation="MKVTPTVLSDKQAAFLVRQRVARLATTDRMGKPHAVPVCFALAR
                     ASIFIALDEKPKDVPVTRLKRVRNILENPDVALIADRYTEAWDLLAFVMVRGRAELLD
                     PDTEEHAAAVRLLRGKYHQYEAMRIDENPVIAIRPERAASWGPLDEPEFSGPRILDTI
                     HGRRSVRRYLQKKVPEDLVERVLEAARWAPSPHGRQPWRFAVLTKEATKERLARAMGE
                     EWRANLEMDGQSVEIVEKRLQGSRSRLLDAPVLVLICLYLEDLDAYPDAERQRGETTM
                     AVQSLGAAAQNALLTAYDSGLDAGWMCAPLFCPERVVEALGLDPSLVPHALLTLGYAD
                     GDPPKRRSRRPLDELVVYRD"
BASE COUNT         1407 a         2319 c         2269 g         1402 t
ORIGIN      
        1 ccgctgttta tagactacga ggaacacctg aacgattacg cggggacgga ccgcgccccg
       61 gtggagcacg aagcggtgag gtactagttg gaaggcgatg taaaaaggct ctctgtgccg
      121 gatgagccaa cagagctaaa ggccgggatc cgtgccctac tcgaagattc gcccgaggag
      181 ggtggccgtg ttatcgccga cgctgccttt gtctgtgatc tcctctggga agaatggaga
      241 gccgacctcg aaggggccgg catgggctac gaccggtttc tcgagatatc gcgctcttac
      301 gcgggcgaat tcaggttctg ggtcgtcggc gaacgtctct gggaccactg cgcggccggc
      361 ctcgccggcc gcttgttgcg tagactcccg gccgggcagg ccactaatct cgcggaggta
      421 aacggatgaa cgtcagcacc gaatacacta ccatcgcccc gcacggcggc gaactcgtcg
      481 accgcagggc gcctgaggag caacgcgaag agctactcag cagggcccag ggcttcgcca
      541 gggtaacttt gggtcccagg gctctctccg acctggagat gatctccacc ggcgtcttct
      601 ctccccttcc tggctttatg gtcagcgagg actacgagag cgtcgtcgag acgatgcacc
      661 tgaaggacgg cctggcctgg agcctgccga tcacactccc cgcgagcgag gaagaggcaa
      721 acggcataag ggttggcgag gagatctccc tctccgatga ggcgggacgg gtgctcgcga
      781 ccatgatcgt cgaggaccgc tactcctacg acaaggagca cgaggccggc gaggtctacg
      841 gtactacgag ccctgaacat ccaggcgtcg ccgccgtcta ccgccagaag gatgtgcttc
      901 tcggcggcga ggtaacgctg ctcgtggatg acccgaaccc gaagcctttc ccgaccttcc
      961 actacgagcc gcgccgtctg cgcgaggtct tcgcggagaa aggctggaag cgcgtcgtcg
     1021 gtttccagac ccgcaaccct gtgcaccgct cgcacgagta catccagaag agcgccctgg
     1081 agatagtcga cgggttgctc ttgaacccgc tcgtcggaga gacccgcgcc gcggacatcc
     1141 ccgccgacgt caggatgcgc tcctacgaga cgatcctgga gcgctactac cccaaggacc
     1201 ggacgatgct ggccgtcttc cccgcggcga tgcgctacgg tggaccccgc gaggcgatct
     1261 tccacgcgat ctgccgcaag aactacggct gcacacactt tatcgtgggc cgcgaccacg
     1321 ccggaccggg caaggactcc agcggcaggc ccttctacgg cgagtacgac gcccaacata
     1381 tcttcgatga gttcgaggaa ggggagatcg ggctgacccc actcttcttc gaacatgcct
     1441 tcttctgcct gcaatgccag ggtatgggga ccaccaaaac ctgccctcac gataaagtca
     1501 accacgtgtt tatcagcggt acgaaggtgc gcgagatgct ttccaaaggc gagtacccgc
     1561 ccccagagtt ctcccgcccc gaagtcatcg aagtgctcat agagggacag cggacatagc
     1621 aggattgtta agaatacgtt aaaatcgcaa cagtaggtaa tcaagagtcg aggagcgtta
     1681 acaatggaga atgggacgag gtttggccgc ggcttcacgc tgtggttcac gggtctatcc
     1741 ggagctggca agacgaccat atccgagatc gtcgagcacg agctcaggaa ccgctttcgc
     1801 aagatagagg ttctggatgg tgacatcgtg aggaccaacc tatccaaggg tcttggtttt
     1861 tccagggagg acaggaacac caacgtcttg aggatagggt tcgtcgccga cttgttgacg
     1921 cgcaatgggg taggtgtgat cgtcagcgcc atctcgcctt tcaaggaggt gcgtgaccag
     1981 gtgcggcgca acatcggggg ggacttcatc gagatcttcg tcgatgctcc actagaggtt
     2041 tgcgccgagc gggacgtcaa gggcctctac gagaaagcct tctcggggga gatcccgcag
     2101 ttcaccggtg tttccgaccc ttacgaggcg cctaccgccc ccgagttgca catcaagact
     2161 gatgaagagg agcccaggga gagtgcggcc agggtcatcg gccacctcga agagttcggc
     2221 tatctcaggc ctgtcgaggc gtttgaccgg gcgtagcctg gtgtgataga tcccacgcct
     2281 gctctcttcg ggttgctcgt cggtttcctc gtaggcctca cgggggtcgg gggtggatcg
     2341 ctcatgacgc cttttctggt ggctgtgatg ggggtgcctg ctcctaccgc cgtcgggacc
     2401 gacctgacct tcgccacctt caccaagctg accgggtctt tccagcattt caggcagcgc
     2461 tcggtcaacg tcgagatcgc cgtttttctg gggatgggca gcatccctgc cgggctgctc
     2521 ggggtcggga ccctggagtg gataaagggc tccttcgatc ccgcgagggt cgagtcgatc
     2581 atgatcacga tcatagcagc gacgctcgtt ctggtagggg tgtccctgat ctaccgcgat
     2641 tacttcatgc caaagaggca cgagggcccg aagcccggcc ggtgggatgg caagagccgg
     2701 atgagccgtc ggcgccgggc ttataccgtc cttttcgggg cccttggcgg ctacttggtc
     2761 gggctcacct ctatcggctc ggggagcgtg atggccgtca tactgctcct tctgtatcct
     2821 atagcaccgg ccgttatagt cggcactgac attacacacg cgatggtcct ctcgttcgtc
     2881 gttggaatag cccacatgac gcagggcaac gtggacttcg ccctggccgg gaccctgctg
     2941 ttgggcagca tccccggtgt ccttatcggg agccggatgg cgccctggat ccccggcaag
     3001 ccgctcaagc tgatactggc tatcatgctg atcttcgtag gtgcgcgcct gcttctcgca
     3061 ggctaaagcg atccggagtt cccgtttaca ggcttccggc ctggcgcatt attccgccgt
     3121 ccacgaagaa tgtgctgcct gtggactagg aagaggcgtc cgaggccagg tacacggcta
     3181 aggaagcgac atcctcggga cgcccgatcc tgcctagcgg aatctctgag agcaactcat
     3241 gtctctgctc gggatgttcg tcgaggttta tgttgatcgg cgtctctatg gcgcctgggg
     3301 cgatgttgtt tacggtgatg ccgtgcgggg ccagctcgac ggcgatggtt cgcataagca
     3361 tcctgacgcc tcctttggcc gcgcagtaag gagagttagt cggcataggc aggtcctcgt
     3421 gcaccgaaga gacgttgatg atgcgccccc catcgccctg tgagaccatc tggctggcag
     3481 cctcctggca accgagccac actcctgtaa ggttgaccga gatcaccttc tcccacacct
     3541 cgaggggcgt ctcgagaaag gggatcttct gttctatgcc tgcgttattg accatcacgt
     3601 cgaggcgacc gaactccttg acggtctgct ggatcaagcg cttcacgcct tccggctcgg
     3661 agacgtcggc ctgcacagag atggccttgc ctccgccctt ctctatctct tggaccgctt
     3721 cctgcccttc ttcggggtga cttctgtagt tgatggccac cgaggcacct tcacgcccga
     3781 aagcgagtgc gatggccctg ccgatcccga gggaagaacc cgttactgcg gcgaccttat
     3841 tctctagctg caatgctaac ctccctatcg gatagtcgga gcgcgcagtt gaccagcgcc
     3901 aggtgtgtaa acgcctgtgg gaagttgcca accaacctct gtgcgcgcgt gtcgtactct
     3961 tcgcttagca gcccaacatc gttacgcagc gcgagcagcc gctcgaagag ttcctcagcc
     4021 tcccggtggc ggcctatgcc ccacagcgcg tagaccatcc agaagctgca gatcaagaac
     4081 gagccctcgc gtcctgtgag accgtcgtcg gattggtctg ttcggtagcg gattacgagg
     4141 ccatcttcgg tcagcttgtt gcggatcgtc tcgatggtac tgaccacccg ctcatcgtcg
     4201 tagggaagaa accccacgcg cgggatgagc agcgcgcagg cgtccagcgc gctggaaccg
     4261 taagcctggg tgaaggtccc ccgtccggcg tcgtagccgt tctccaggat gtcggagtga
     4321 atctcgtcac gcacggcgcg ccagagctcc acgggaccat cgagcccgaa acgctcgacg
     4381 cccttgacca aggagtcaaa cgccacccag cacatcaccc tggagtacac gaagtgctgc
     4441 ctctctccgc gcgactccca gatcccgttg tcaggcctct tccaggccac ttgcaggcgc
     4501 cctgcgagcc aacgctgcag ttcccaacct ctctccatag agacgatccc cgcggtccga
     4561 cccacgtgcg cggcggcgag cacctcgcct ggtacgtcgc cctggacctg gcctgcggcg
     4621 tcgttgccta tgcgcaccgg cgaggcgcct tcgtagccgg ggagccaggg caattcatac
     4681 tcgggcaggc ggcgggcgcc gtcgatgccg tacatgatct gagtgtcggc agggtcgcca
     4741 gcgacagccc ttagcaccca ttgctgccag cagctggcct gctcgaggag gccagctcct
     4801 atcagcgcac cgagggtgaa agtgctatcg cgcagccagc agtaacggta gtcccagttc
     4861 ctggagccgc cgagggtctc gggaagcgag gtcgtggctg cggcgacgat gccgccgctc
     4921 ggctcgtagg tcagtccttt gagggttagg agagagcggt acacagcatc ttggaagcga
     4981 ccttcgtagc tgatgccgcc ggcccaatcg agccaaaagc gttccgtgtc gtccaccgcc
     5041 tgcaggccgt tgatggcaag aggcctctcg gcgtaggaag gcgaccaggt gagtacgaga
     5101 gggacgcgct ctccctcgga aaccgtgaac cgggagagga tctcaccgcc cacctcctcg
     5161 tggggccctg aggcgcgcaa ccacagcgcg tccgggcccg ctacggcgca cctcacgtcc
     5221 ttgtaaggtg acccaagggg cgacgtgccc gtagtcgaaa cgaatcctta gctcgcttgt
     5281 gaccgatact ttgcccctta caccctcgac cacccgaacc agatcgggcg ccacaccgcg
     5341 gggcggcata caatcgacta ccctcaccgc gccgcccgga gtttcccact cggtctccag
     5401 gacgagcgtg tgccccctgt agcggtgcct cgtgcagcgt ccgcccgacg cgggggcgat
     5461 ccgccagcgg ccgttggtgt cctcgccaag caatgcggcg aagcaggccg gcgaatcgaa
     5521 gcgggggaag cacagccagt ccacagagcc gtcggagcca accagggccg cggtctgcag
     5581 gtctcccacc atcgcgtagt cttcgatcct cccaggcatc agacccttct ccgtacccca
     5641 gtgtcgcaag atttacggcc agtaaactat gccagaggct gggatatgcg gccacccgca
     5701 gcagccgtgt ggggaagacc tgaggccttc ccaggcctga catcaggcta caggtgtcac
     5761 gatttgcagg gatagccgca ttgctaagga tgctctgcca tatcctggct ctgctccaat
     5821 gacaacatga tctcgcttcg gccaacgaca tacccgatcc ggactttctc tcggtcggac
     5881 agaagatctg ctactgatct tggccctggt gcgcgcgtct cttgtagact aagtgcgtga
     5941 gcgagcacac gctgaggaga cacttttggt agggaacggc accgtcccga cgttcgggtt
     6001 gtgttatgac ttcaggcaga agttgccgct cgggcactac gcgcggttct atgccgagtg
     6061 tctggaggcg tcctattacc acctctgctt ctggggacgt ctccccggtg tcacccatga
     6121 acaggcgctg gccaacatgc gtctgttcgc ctccgatgtc atcccgcgcg tccgcgacgc
     6181 agtaaagatt tgaaggtaac gccgacggta cttagcgaca aacaggccgc cttcctcgta
     6241 cgtcagaggg tggcccgcct cgctaccacc gaccggatgg gcaaaccgca cgcagtacct
     6301 gtgtgcttcg ccttggcgcg tgcttcgatc ttcatagccc tcgacgagaa gccaaaagat
     6361 gtacccgtaa cccgtctcaa gcgcgtcagg aacatcctcg agaaccccga tgtagcgctc
     6421 atcgccgacc gctacaccga agcgtgggac ctcctcgctt tcgtgatggt gcgtggcagg
     6481 gctgagcttc tggaccctga caccgaggag cacgccgcag ccgtgaggct cctgaggggt
     6541 aaataccacc agtacgaggc gatgcgcata gacgagaacc ccgttatcgc catacgtccc
     6601 gagagagccg cctcgtgggg gcccctcgac gagccggagt tctccgggcc caggatcctc
     6661 gacacgattc atggccgccg ctccgtcagg cgttatctcc agaagaaggt gcccgaggat
     6721 ctcgtggagc gggtactcga agccgccaga tgggcccctt ccccccacgg acgacaaccc
     6781 tggcgattcg ccgtgctcac caaagaagcg accaaagagc gtctggcgcg cgcgatgggc
     6841 gaggagtggc gggccaacct ggagatggac gggcagagcg tggagatcgt cgagaagcgc
     6901 ctccaaggct cccgcagtcg cctgcttgac gcgccggtac tagtcttgat ctgcctttat
     6961 ctcgaagatc tcgacgctta ccccgatgcg gagcgccagc gcggtgagac aaccatggcc
     7021 gtacagtctt taggggctgc cgcccagaat gcgctgctga ccgcctacga ctcgggcctc
     7081 gatgcgggct ggatgtgcgc cccgctcttc tgccctgaga gggtagtcga ggcgctgggt
     7141 ctcgatccga gcctcgtacc gcacgcgctg ctcacgttgg gatacgccga tggtgatccc
     7201 ccaaagcgcc gctcgcgtag acctctcgac gaactcgtag tctacaggga ctgagcacag
     7261 ccgatatggt catgcagagg cgcctgaact taaccgaccg ttccctaaca taaactcgac
     7321 aaaccttatg ggagatacgc tagaatgtgc gctcgtgcat aatggagtga ggaggatcta
     7381 tgacggagca ggagata
//
LOCUS       DATMJA010000113         2515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_548560, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000113 DATMJA010000000
VERSION     DATMJA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548560"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1972
                     /locus_tag="VNT12_02595"
     CDS             <1..1972
                     /locus_tag="VNT12_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWI45034.1"
                     /translation="KPVLLSVGYSACHWCHVMERESFEDDETARMMNEHFVNVKVDRE
                     ERPDIDSIYMAAVQALTRHGGWPMTVFLTPDGAPFYGGTYFPPIPSRGMPSFQQILLS
                     VVDAYENRREEVLQNAEAVRDYLQASTAAVMPEAESAGTELLDRAAGALLSQLDRRFG
                     GFGGAPKFPQAMNLEVLLRHYHRTGERSALDGVELTCRQMENGGIYDQLGGGFSRYSV
                     DAYWLVPHFEKMLYDNALLSRLYLETYQATGDAFYRRIAEETLDYVLRDMTSEEGGFY
                     SAEDADSEGEEGKFYVWTPEELEAVLDPDEASLAARYWDVTERGNFEGKNILHVARPP
                     EAVADEFGLSTEELWARIVTIRAKLFAVREERVRPGRDEKILAAWNGLMLRSFALAAR
                     VTGREDYREAAIENATFIVEKLKEDGRIHRSYNDGRARFNGYLEDYAMVADGLVALYE
                     ATFETRWIVEADALLDAVTELFWDEEKRAFYDTPADHEKLVTRPRDVYDNATPSGTSV
                     ATEVLLKLALFLDRGDYRQRAEDVLEKLAGGMEKVPSGFGRLLCALDLSISETREVAI
                     VGDPEAQDTRDLLETVYAGYLPNKVVAGRGPDDDEAGGLIPLLAQRPAREGKATAYVC
                     VHFACQTPTTDPEELSRQLGVSDDGGQISGGL"
     gene            complement(2070..2420)
                     /locus_tag="VNT12_02600"
     CDS             complement(2070..2420)
                     /locus_tag="VNT12_02600"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HWI45035.1"
                     /translation="MIHEPLRTVEREVASWPGVSIGDTGRGGLQLNYGKVELGHLHGS
                     SFADLPFPKKIRDELIAEGRASVHPPLPDSGWVRRRMDGPGDAEAVIELFRMNYERAQ
                     ARAKRRAASESTGR"
BASE COUNT          458 a          868 c          747 g          442 t
ORIGIN      
        1 caaacccgtc cttctctcgg tcggctacag cgcgtgccac tggtgccacg ttatggagcg
       61 tgagtccttc gaggacgatg agaccgcgcg catgatgaac gagcacttcg tgaacgtaaa
      121 ggtagaccgc gaggaaagac ctgacatcga ctctatctac atggccgccg tccaggccct
      181 cacccggcac ggcggctggc cgatgaccgt cttcctcacc cccgacggtg ccccgttcta
      241 cggcggcacc tacttcccac ccatcccttc gcgtggcatg ccttccttcc agcagatcct
      301 cctcagcgtc gtcgatgcct acgagaaccg gcgtgaagag gtcctgcaaa acgccgaggc
      361 cgtccgcgac tacctccagg cttcgacggc ggccgtcatg cccgaggccg agtccgcggg
      421 aaccgagttg cttgaccgcg cggcaggcgc cctcctctcc cagctcgaca ggcgctttgg
      481 gggcttcggc ggcgcgccca agttccccca ggcgatgaac ctggaggtcc tgctccgcca
      541 ctaccaccgc acaggagaga ggtccgcgct cgacggcgtg gagctcacat gccggcagat
      601 ggagaacggt ggcatctacg accagcttgg cggaggcttc tcccgctact ccgtggacgc
      661 ttactggctc gtcccgcact tcgagaagat gctctacgat aacgccctcc tctccaggct
      721 ctacctcgag acgtaccagg ctacaggaga cgctttctac cgccgcatcg ccgaggagac
      781 gctggactac gtcttgcgtg acatgacgag cgaggaaggg ggtttctact ctgccgagga
      841 cgccgactcc gagggcgagg agggcaaatt ttatgtttgg acgcccgaag agctggaggc
      901 cgtcctcgac cctgacgaag cgagcctcgc ggcgcgctac tgggacgtca ccgagcgggg
      961 caacttcgag ggcaagaaca tcctgcacgt cgcccgccca cccgaggccg tcgcagatga
     1021 gttcgggctc tccacggagg aactgtgggc ccgtatagtt acgatccggg cgaagctctt
     1081 cgccgtgcgt gaggagcgtg ttaggccggg acgcgacgag aagatcctgg ccgcctggaa
     1141 cggcctcatg ctccgctcct tcgctctcgc cgcccgcgtt acaggccgtg aggactaccg
     1201 tgaggccgcc atagagaacg cgacctttat cgtggagaag ctcaaggaag atggccgcat
     1261 ccaccgctct tacaatgacg gtcgggcaag gttcaacggc tacctggagg actacgcgat
     1321 ggtcgcggac gggctcgtcg ccctctacga ggccaccttc gagacgcgct ggatcgtcga
     1381 ggctgacgcc ctccttgacg ccgtcaccga gctcttctgg gacgaggaga agcgtgcctt
     1441 ctacgacacc ccggccgacc acgagaagct cgtcacccgc ccacgcgacg tctacgataa
     1501 cgccacaccc tcaggaacct ctgtcgccac cgaggtcctg ctcaagctcg ccctcttcct
     1561 cgaccgtggc gactaccgcc agcgcgccga ggacgtccta gaaaagctcg ctggcggcat
     1621 ggagaaagtc ccaagcgggt tcgggcgcct cctctgcgcc ctcgatctgt ccatctcgga
     1681 aacccgcgag gtagccatag tcggcgaccc tgaagcgcag gatacgagag acctgttaga
     1741 gacggtctac gccggctatc tacccaacaa ggtcgtcgcg ggccgcgggc ccgatgacga
     1801 tgaggctggc ggcctgatcc ccctcctcgc ccaacgcccc gcccgcgaag gcaaagccac
     1861 agcctacgtc tgcgtccact tcgcctgcca gaccccgacc accgaccccg aagaactctc
     1921 gcggcagctc ggcgtttctg acgacggggg acagatctcc ggagggctct gagcgccacg
     1981 cctacatcac gccggggcga gatggccctc atgccttacg atctcgaagc ccaacaccct
     2041 ccggtagtac taattctatc cccaggagat catctcccgg tggactctga cgccgcgcgc
     2101 cgtttggcac gggcctgtgc acgttcgtag ttcatgcgga agagttcgat cacggcttcc
     2161 gcgtcgccgg gaccatccat cctccggcgt acccaacccg agtcgggcag cggcggatgc
     2221 accgaggccc gtccttccgc tatcagctcg tcacgtattt tcttgggaaa gggcaggtca
     2281 gcgaagctcg agccgtgcag atgcccgagc tccaccttgc cgtaattgag ctgaagtccg
     2341 cctctgccgg tgtcgccgat actgacgccc ggccagctcg caacctcgcg ctcgaccgtc
     2401 ctcagcggtt cgtggatcat cagatccctc cttctatgag cttgcctcat tcgtctttat
     2461 aatgcgggaa ggggactgat gcatcgtgca gggggacggt tttcggcctg gacct
//
LOCUS       DATMJA010000114         3113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_74776, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000114 DATMJA010000000
VERSION     DATMJA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74776"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /gene="alaS"
                     /locus_tag="VNT12_02605"
     CDS             complement(<1..1249)
                     /gene="alaS"
                     /locus_tag="VNT12_02605"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWI45036.1"
                     /translation="MQSSEIRRRFLDFFGERGHRLYPSASLVPHNDPTVLLTTGGVQQ
                     FIPYFLGQERPPSTRAMSVQKCFRTPDIEDVGDWHHLTFFEMLGNFSFGDYFKKEAIE
                     WAWAFVTEELGIEPERLWVTIFEGDEDAPEDVEAKEYWMGVGVPEERIFGLPKSENWW
                     GPPGETGPCGPCSEIYYDHGEECAKGDPAEDPKYGPGGDEGDPRFLEIWNLVFNQYEQ
                     LKDKSFVPLSKTGIDTGMGLERTTAAVQDVLYVYDTDLYAPIFEEAKKYTGVSLGDSE
                     TTDRALRIVADHARGMAFLIADGVRPGNQRREYVLRRIIRRAMLQAHTRLGMSPSQLA
                     GLAEVVVDYMGDFYEELRSAREDIGRIVTGEAKRFVEIYESGMSLLASEIERMRGGHF
                     PGDVAFMLHDTYGFPVEVTREVLA"
     gene            complement(1239..2051)
                     /locus_tag="VNT12_02610"
     CDS             complement(1239..2051)
                     /locus_tag="VNT12_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HWI45037.1"
                     /translation="MSRRYRRPDKARTRRLLVRYHKHGDQNARERVIQEQLPLAEFLA
                     RKFAGRGEPLDDLIQVASVGLIKAVDRFDVDRKIEFSTYATPNILGEIKRYFRDKGWA
                     MRVPRGLQELRQSAKEAIRNETVKTGRSPTLQELAEMLDADVEGVAEALTLGRAYNTA
                     SLDAPVSQDEPEGDTIMDFQADDVSPIEGLEDRILLQEAIDGLKDQQQEILKLRFNEG
                     KTQTEIADHIGVSQMHVSRLLRRALEDLRVELEHLEHERETSKLSPDGAHAE"
     gene            complement(2048..2335)
                     /locus_tag="VNT12_02615"
     CDS             complement(2048..2335)
                     /locus_tag="VNT12_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45038.1"
                     /translation="MTEDHPQEYAYLRLPPHEELRSCVGLVLAGMAARARVGVGGLEE
                     AVEVLEGFHSDDAPTRFRFSLAGDGVLAEVEEPASAGSTQTSWRTVVELVS"
     gene            complement(2349..2669)
                     /locus_tag="VNT12_02620"
     CDS             complement(2349..2669)
                     /locus_tag="VNT12_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45039.1"
                     /translation="MASWGRVVAGVVIGVAGTIYATNEDVRKNLPKAARDLPDNVRRR
                     YRSAVAAAREASARRREEILHDLEEHGGGNHAARGVKPDPDAADAPMSTSDEDTSTDK
                     PEQR"
     gene            complement(2672..3052)
                     /locus_tag="VNT12_02625"
     CDS             complement(2672..3052)
                     /locus_tag="VNT12_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HWI45040.1"
                     /translation="MELFVELMKGLLFAGIAVAFFVLAWMLLRLARIFSTTEQTIKDV
                     SGQVVPLLGKTHITVDNINSQLTQLDEAVGDVAGMTGELDQTTAVITRGVRGGVIKVS
                     SATAGISRGVSTFLGGEKRPEKEE"
BASE COUNT          513 a         1051 c          919 g          630 t
ORIGIN      
        1 cggcgagcac ctcccgcgtc acctccacag ggaagccgta cgtgtcgtgc aacatgaagg
       61 ccacgtcacc agggaagtgt ccgccccgca tccgctctat ctcggaagcc agaagcgaca
      121 tcccggactc gtagatctcg acgaacctct tagcctcgcc agtgacgatg cggcctatgt
      181 cctcacgcgc ggaccgtaac tcctcgtaga agtcgcccat gtagtcgacc acgacctcag
      241 ccagcccggc tagctggcta gggctcatgc cgaggcgcgt atgtgcctgc aacatcgctc
      301 tgcgtattat gcggcgaagt acgtactctc tgcgctggtt ccctggcctg acgccgtcgg
      361 cgatgaggaa ggccatgcca cgagcgtggt cggcgacgat gcgcagcgcg cgatctgtgg
      421 tttcggagtc tcccaaggag acgcccgtgt acttctttgc ctcttcgaag atgggtgcgt
      481 agaggtcagt gtcgtagacg tagagaacat cctgtaccgc ggcggtggtc cgttccaaac
      541 ccatgccggt gtcgatacca gtcttgctca agggcacgaa ggacttgtct ttcagctgct
      601 cgtactggtt gaagacgagg ttccagatct cgaggaagcg cggatctccc tcgtcgccgc
      661 cgggaccgta cttcgggtcc tccgccgggt cgcccttggc acattcttca ccgtggtcgt
      721 agtagatctc cgaacaaggg ccacacggcc cggtctctcc tggcggtccc caccagttct
      781 cgctcttggg gaggccgaag attctctctt cgggcacgcc gacacccatc caatattcct
      841 ttgcctccac gtcctcgggg gcgtcttcgt cgccctcgaa gatcgttacc cacaggcgct
      901 ccggctcgat gcccagctcc tccgtcacga acgcccaggc ccactctatg gcttccttct
      961 tgaagtagtc cccgaacgag aagttcccta gcatctcgaa gaaggtgagg tgatgccagt
     1021 ctccgacatc ctcgatgtca ggggtacgga aacacttctg cacgctcatc gcccgcgtgg
     1081 aaggtggcct ctcctggccg agaaagtagg ggatgaattg ctggaccccg cccgtggtga
     1141 ggaggaccgt ggggtcgttg tgggggacca gggacgcgct cgggtagagc ctgtggcccc
     1201 gctccccgaa aaagtccagg aaccgtcgcc ttatctcgct actctgcatg cgccccatcc
     1261 ggggacaatt tgctggtctc gcgctcatgt tccaggtgct ccagctcgac ccgcaggtcc
     1321 tccagtgcgc gtctgaggag gcgggagacg tgcatctggc tgacgcctat gtggtccgcg
     1381 atctccgtct gcgtcttgcc ctcgttgaag cggagcttca ggatctcctg ctgctggtcc
     1441 ttgaggccat cgatggcctc ctggagcagg atcctgtcct ccaacccttc tatcggggac
     1501 acatcgtcgg cctggaagtc catgatcgtg tccccctcag gctcgtcctg gctcactggc
     1561 gcgtcgaggc tggccgtgtt gtaagcccgc ccgagggtca gcgcctccgc cacaccctcg
     1621 acgtcggcat cgagcatctc ggcgagctcc tgcagggtgg gcgaccgacc cgtcttgacg
     1681 gtctcgttgc ggattgcttc cttcgccgac tggcgcagct cctgcagacc gcgggggacg
     1741 cgcatcgccc agcccttgtc acggaagtag cgtttgatct cgccaaggat gtttggggtc
     1801 gcgtaggtgg agaactcgat tttcctgtct acatcaaagc ggtccaccgc ctttatcagg
     1861 ccgacggagg cgacctggat cagatcgtcc agaggctccc cgcggcctgc gaacttgcgg
     1921 gccaaaaact cggccagggg aagttgttcc tggataaccc gctcgcgggc gttctggtcg
     1981 ccgtgcttgt gatagcgcac gagcaggcgc cgcgtgcgcg ctttgtccgg tctgcggtag
     2041 cgacggctca cgagaccagc tcgactacgg tgcgccagct cgtctgtgtg ctccccgcgg
     2101 aggccggctc ctcgacctcg gcgagaaccc cgtccccggc cagggagaag cgaaagcggg
     2161 ttggagcatc atcggagtga aagccctcaa gaacctccac ggcctcctcc aatcctccca
     2221 ctcctaccct agcccgtgcc gccatccctg ccagcaccag gccgacgcag gaccgcagct
     2281 cttcgtgggg cgggagcctc aggtatgcgt actcctgggg atgatcttcg gtcaaggcaa
     2341 tcccttcctt agcgttgctc gggcttgtct gtggatgtat cttcgtccga tgtgctcatc
     2401 ggggcgtccg cggcgtcggg atccggcttt accccgcgcg cggcgtggtt cccgccgccg
     2461 tgttcctcga ggtcgtgcag gatctcctcc cgccgccttg cagaggcctc gcgcgccgcg
     2521 gccaccgcgc tcctgtagcg gcgacggacg ttgtcgggca gatctcgcgc cgctttaggc
     2581 aggttcttgc gtacgtcctc gttcgtagcg tagatcgtgc ctgccacccc tatcacgacg
     2641 ccggccacga ccctacccca gctcgccacc gctactcctc cttctcagga cgcttttcgc
     2701 cgccgaggaa ggtgctgacc ccgcgcgaga tacccgccgt cgcagaggag accttgataa
     2761 caccgcccct tacgccgcgt gtgatcacag ctgtagtctg gtcgagctcg cccgtcatgc
     2821 ctgcgacgtc cccgacggcc tcgtcgagct gcgtgagctg gctgtttatg ttgtccaccg
     2881 tgatgtgcgt cttccccaga agcggaacga cctgccccga gacgtccttg atcgtctgct
     2941 cggtggtcga gaagatcctc gccagcctca acaacatcca cgcgaggacg aagaacgcga
     3001 cggctatccc cgcgaagagc aaccctttca tcagctctac gaataactcc aaaccctaaa
     3061 gcacctccgt ccggggtccc tggaaaccca gattatagct gtgattgcac gca
//
LOCUS       DATMJA010000115         4885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_74831, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000115 DATMJA010000000
VERSION     DATMJA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74831"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VNT12_02630"
     CDS             <1..997
                     /locus_tag="VNT12_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWI45041.1"
                     /translation="AYIAESAPVSIRGSMLVTFQIATTSGIAVAYFVGAALAPTESWR
                     LILSLSAIPAVIVLALIIRLPDTARWLLMNGRREEATDLLRRVEPDMDPDEEVRRIEE
                     DLSYEEKGSFAELFQGRFRKAGIFVVGLGFLVQITGINAIVYYSPTIIQEVGVTSPTG
                     AIVATGFVQIAGVIAEVVAFLVVDRWGRRPTLLTGIGIMGAANAILVLAYALNSPATF
                     TLLGILLFTVGFNFGYGSLVWVYASESFPVRLRTQGGSAMLTADLFANFIVGVVFLGA
                     LGALGGSVTFGIFFALALVSFAFVYVLAPETKGRPLEAIRTYWYNNGRWPEESST"
     gene            1340..1765
                     /locus_tag="VNT12_02635"
     CDS             1340..1765
                     /locus_tag="VNT12_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWI45042.1"
                     /translation="MRQLAAVLLSCALFSVVLLAGSPSEAYAQGYSGQAVVEDAQSYI
                     GAPYGTWGMDCSGFTSAVFADLGVYLPDSPDAQYAYGTPSYGEAGDLVFFDEAGYGIS
                     HVGIATGYGTVIHASTYYGAVVETPIEYIPGYVGSVDAY"
     gene            2012..2986
                     /locus_tag="VNT12_02640"
     CDS             2012..2986
                     /locus_tag="VNT12_02640"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWI45043.1"
                     /translation="MRPELVLRGVIPANLMAFDSELEIDEVNYRRHLRYLVDTPGVTG
                     ITTNAHASEVATLTFEEQQRVLEMTLDEVAEAVPVVCGVYQDGTSKAARIAKSAERQG
                     ADCLLVFPSTVYDFGHQLRPEMAYGHIAQIAEAVSLPIIVFVYPVASGLHIPTDNLVR
                     ICDEIDNVIAVKEWSNDIGVYERNWRELKALDKEISLLSSFSKSLLASLCIGADGILS
                     GHGSVIADLHVEMFEAVQRGDLAGARRVAARIYPVVQATYAEPFLDGHNRMKEALAIL
                     GRIDEAHVRPPLQRISEAEREKIRRVVTEAGLPGARREQPDAALGRRG"
     gene            3122..4303
                     /locus_tag="VNT12_02645"
     CDS             3122..4303
                     /locus_tag="VNT12_02645"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI45044.1"
                     /translation="MLNGVKVLSFTHFLQGPSAVQILADLGADVVKIEPTKGAWERHW
                     SGAEAYLNGVSVFFLLSGRNQRSLGVDLRSERGKEIVWRLIRDSDVIVENYRPGAMER
                     LGFGYKEVSEEKPDLVYCSLSGYGTDGPYRDRAGQDLLSQAMSGLATISGRKSDPPTL
                     VGSAIVDQHAAVLGALGVLAALYERRDTGKGKRVDSNLLNSALDLQIEPFIYHLNGHL
                     YDRSETGVSSRFHQAPYGIFETADGYICISLTPPEKMAEAFGDDTFHEWTEEDQFRRR
                     EEVNAHIAGHVRTRGAEHWYGVFDEVGIWYAPVNDYEDVEEDPQVVHNQAILTFEHPR
                     AGTVRVLSHPVRYDGEAPPLRILPPDVGEHTEEVLSELGYGQAEIENLKAEGVVRAEE
                     H"
     gene            4287..4736
                     /locus_tag="VNT12_02650"
     CDS             4287..4736
                     /locus_tag="VNT12_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWI45045.1"
                     /translation="MQRSTDTNKAVDRTIPLEVGDSVAFSKTIGESDVYLFAGITGDF
                     SPNHVNKEVMSGTPYKERIAHGVLSIGFASTTSTLMIEKSGGKAVSLGYDRIRFVGPI
                     FIGDTITVTYTISEIDEENLRTRANIEVANQRGEVCTVAQHILKFLA"
BASE COUNT          916 a         1509 c         1554 g          906 t
ORIGIN      
        1 ggcatacatc gccgagtccg cgccagtcag cattaggggc tcgatgctcg tcaccttcca
       61 aatagcgaca acctcgggta tcgcggtcgc ctacttcgtc ggcgcggccc tcgcgcccac
      121 cgagagctgg cgtctcatac tctccctctc cgccatcccg gccgtcatcg tgctggccct
      181 catcatccgc ctgccggaca cagcccgctg gctcctgatg aacggccgac gcgaggaggc
      241 gaccgacctc ctgcgcaggg tcgaacccga catggacccc gacgaggaag tccggaggat
      301 cgaggaagac cttagctacg aggagaaggg gagcttcgcc gaactcttcc agggacgttt
      361 ccgtaaggcc ggcatcttcg tcgtgggcct tggctttttg gttcagatca caggtatcaa
      421 cgccatcgtc tactatagcc cgacgattat ccaggaggtc ggcgtcacga gtccgacggg
      481 cgcgatcgtc gcgacaggct tcgtccagat tgcgggtgtg atcgccgagg tcgtcgcctt
      541 cctggtcgtg gacaggtggg ggaggcgacc cacgctcctg accggaatcg gcattatggg
      601 ggccgccaac gccatcctcg tcctggccta cgcgctgaac tccccggcga cgttcacctt
      661 gctcggcatc ctgctcttca ccgtgggctt caacttcggc tacggttcgc tggtctgggt
      721 ctacgcttcc gagagcttcc ccgtgcgcct gcgcacccag ggcggctcag ccatgctgac
      781 agcagatctg ttcgccaact tcatcgtcgg agtcgtgttc ctgggcgccc tgggcgctct
      841 aggcggctcg gttactttcg ggatcttctt tgccctggcc ctggtctcgt tcgccttcgt
      901 ctacgtactc gcccccgaga ccaagggacg accgctggaa gcgatccgca cctactggta
      961 caacaacgga cgctggcccg aggagagcag cacctaagcc ttccgtaaga ccgggatcag
     1021 acccttcatc tccctccgcc tggtgttagt accagggatg gtttttgggc aaatcaggga
     1081 actgctcttc ttatccaagg gctctgtcag cccgaggttt gcaccgggcg tgcggccctc
     1141 gcgggcgcgc ggatcacgaa gaggcgcgcg cgtacgcggg taagatctac gaccgtcgtc
     1201 gggtttagca ccttcgtgat accgggtgtc ttcacgccgc gagcggccca ttactgcgcc
     1261 ggatccgtct tgaaaccgcg cagatccctg gtagcatcca ccgccgttgt gcaagcaaga
     1321 taattggagg gggatataga tgcggcaact tgctgctgtc ctgctttctt gtgccctgtt
     1381 ttcggtcgtc ctactggcag gctcaccttc cgaagcgtac gcccagggct actcggggca
     1441 ggcggtcgtg gaagacgccc agtcctacat cggtgcgccc tatggcacct ggggcatgga
     1501 ctgctcgggc ttcacctcgg cggtgttcgc cgacctcggc gtgtacctac ccgactcccc
     1561 ggacgcccag tacgcttacg gtacgccttc ctacggggaa gccggcgacc tggtgttctt
     1621 cgacgaggca ggctacggta tctcgcacgt cggaatcgca accgggtacg ggacggtgat
     1681 ccatgcttcg acctactacg gggctgtcgt ggagacaccc atagaataca tcccaggcta
     1741 cgtgggctcc gtcgacgcat actagtgcaa agaggtccaa gacctctttg cacgcatttc
     1801 agcacgctca ggcttcgcct tcgcttgtca gcacttcagc ttgtcagctc tttggtctcg
     1861 ctcgagcgac tgggcgattc gcagtaggac atgacctctg cgaatcgctt tgtgatgctc
     1921 gggtttccga gacagaagac tttgtgaaag aggccggttg ggatgctaag gtaactggcg
     1981 tggtaaacgc ggagcgagga gaggttttcc tatgaggccg gagctggttc tcaggggtgt
     2041 catacccgcc aatttgatgg ctttcgattc cgagttggag atcgacgagg tgaactaccg
     2101 caggcatttg cggtacctgg tcgacacgcc cggggtgacc ggcatcacca ccaacgccca
     2161 cgcttcggag gttgcgacgc tgaccttcga agagcagcaa cgtgtcctcg agatgacgct
     2221 ggacgaggtg gcggaggccg tgcccgtcgt ttgcggcgtc tatcaggacg gaacgagcaa
     2281 ggctgccagg atcgcgaaat ccgccgagcg gcagggggct gattgccttc tggtgttccc
     2341 atccacggtc tatgacttcg ggcatcagct aaggcccgag atggcgtacg ggcacatcgc
     2401 ccagatcgcc gaagccgtga gcttgcctat tatcgtgttc gtctacccgg tggcgtcggg
     2461 gctgcacatc ccgaccgaca acctggtgag gatctgcgac gagatagaca atgtcatcgc
     2521 cgtcaaagag tggtccaacg acatcggcgt ctacgagcgg aactggcgcg aactcaaggc
     2581 actggacaag gagatctcac tgctctccag cttcagcaag tcgctcctgg cgtcgctgtg
     2641 catcggggcc gacggcattc tctccgggca cggtagcgtc atcgctgatc tgcacgtgga
     2701 gatgttcgag gccgtgcaac gtggcgacct cgcgggggcc cgcagggtgg ccgccaggat
     2761 ctaccccgtg gtgcaggcga cctacgccga gccgttcctc gacgggcaca acaggatgaa
     2821 ggaggcgctg gcaattctgg ggcggatcga cgaggctcat gtgcgtcccc cactgcaacg
     2881 catctcggag gccgagaggg agaagatccg acgcgtcgtc accgaagccg ggctgccagg
     2941 cgcacgcagg gagcaacccg acgccgctct cgggagacgc ggctgagatg ccgtaaacgg
     3001 cctcctctct tccccagcct ctcgctgtgg ctcgggcccc cgggaccagc gatgaggtcg
     3061 aggtataggg gatttgttcg ccacggtttg ccgaccgctc acggaaaggg ccggatagac
     3121 aatgttgaac ggggtaaagg tccttagctt cacgcacttc ctgcaaggcc cctccgccgt
     3181 ccagatcctg gccgacctgg gggctgatgt cgtgaagatc gaacccacca aaggggcctg
     3241 ggagcgacac tggtcggggg ctgaggcgta tctgaacggg gttagcgtct tcttcctcct
     3301 tagcggacgc aaccagcgca gccttggtgt ggacctgcgc tcagagaggg gcaaggagat
     3361 cgtgtggcgt ctgatccgcg attccgatgt catcgtcgag aactacaggc caggcgctat
     3421 ggagcgcctg ggcttcggat acaaagaagt ctccgaggag aagcccgatc tcgtgtactg
     3481 ctccctctcg gggtacggca cggacggccc gtacagggac cgggcgggcc aggacctcct
     3541 gagccaggcc atgagcggcc tggcgacgat cagcggccgc aagagcgacc cgcccaccct
     3601 ggtcggctcc gccatcgttg accagcacgc ggcggtgctc ggggctctgg gcgtccttgc
     3661 cgccctctac gagcgtcggg acacgggtaa agggaagcga gtcgacagca acctgctgaa
     3721 ctcggcgctg gacctgcaga tcgaaccgtt catctaccac ctcaacgggc atctctacga
     3781 ccgcagcgag acgggcgtct cctcgcggtt ccaccaggcg ccgtacggca tcttcgagac
     3841 ggccgacggc tacatctgca tctcgcttac gccgccggag aagatggccg aggcgttcgg
     3901 cgacgatacc ttccacgagt ggaccgagga ggaccagttc cgcaggaggg aagaggtgaa
     3961 cgcccacata gcgggacatg tcaggacgag aggggcggag cactggtacg gggtgttcga
     4021 tgaggtcggc atctggtacg cgccggtcaa cgactacgag gatgtcgagg aggacccgca
     4081 ggtcgtccac aaccaggcga tattgacctt cgagcacccg agggcgggca cggtgcgggt
     4141 gctctcccac cctgttcgct acgacggcga ggcgccgccg ctgcgcatcc tgccccccga
     4201 cgtcggagag cacacggagg aggtactctc cgagctgggc tacgggcaag cggagatcga
     4261 aaacctgaag gcggaaggag tcgtacgtgc agaggagcac tgacacgaat aaggcggtag
     4321 atcggaccat acccctggag gtcggcgact cggttgcgtt ctccaagacc atcggcgagt
     4381 ccgacgtgta cctgttcgcc ggcatcacgg gcgacttcag cccgaaccac gtcaacaaag
     4441 aggtcatgtc agggacgcca tacaaggagc gcatcgccca cggcgtgctc tccataggct
     4501 tcgcatcgac gacctcgacg ctcatgatcg agaagtcggg gggcaaggcc gtctctttgg
     4561 gctacgaccg cataaggttt gtagggccga tctttatcgg ggacacgatc acggtcactt
     4621 acactatctc cgagatcgac gaggagaacc tgaggacgcg cgcgaatatc gaggtcgcca
     4681 accagcgcgg cgaggtgtgc accgtggccc agcacatcct caagttcctg gcatagagag
     4741 acacaccgtt tgatccgccg acggcatgct aggcaagggg ccgcgccccc tcgtagcact
     4801 tcgtactaag ctgaaatgct gacaggctgc cgcaggcggc gcgttgaatt gctgaaggga
     4861 aggaaaatgc tagaggacaa cgtca
//
LOCUS       DATMJA010000116         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_548648, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000116 DATMJA010000000
VERSION     DATMJA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548648"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VNT12_02655"
     CDS             complement(<1..836)
                     /locus_tag="VNT12_02655"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI45046.1"
                     /translation="MADHTTVAVLGTGIMGAAMARNLLSAGMEVRAWNRSREKAEPLE
                     KEGAEVADNPGDAARGADFILTMLPDADVVEETVGKDVLSALAEDGVWLQMSTVGEGG
                     NERLAGLAGDSGVAYVDAPVLGTKQPAEQGKLVVLASGPEKVRERSEQVFSAVGSKTV
                     WLGEAGEGSRLKLVVNNWIVGLLGVLAETVAFAEAIGVDPAKFLETIEGGPLSLPYAQ
                     MKGTMMVEEDFPTSFSARLARKDAGLVLDAAEAHDLRMRIAEAVVARFDKAIEAGHGE
                     ED"
     gene            947..1909
                     /locus_tag="VNT12_02660"
     CDS             947..1909
                     /locus_tag="VNT12_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI45047.1"
                     /translation="MRSNTQNAQPALEAKNISRLYKSSGRGVSDVTLRLERGEVYGLM
                     GPNGSGKSTLLRVLSTAIAPDSGGFWVNGVGGLKEKREVRARMGLMVDRPTHYGDLTG
                     LANAYFFARAYGMEEAKAEAALAELFEYFDLAEYADDRVKTYSYGMGKKLALIEALAH
                     GPEVLLLDEPSLGLDYTSQLAYQSRIRDLAGSGVAVLLASNQVDEVEALCDRAAFLHR
                     GEVVAEGTPEDLVSRVVGVRRIVVTLRNPVEYGRVAEVVGVERVVPEGRDLVFHCEER
                     PGIVADVVAGIVRSGGDIVNLSVQRPNLEDVFVELTGEALRDEV"
     gene            1899..>2137
                     /locus_tag="VNT12_02665"
     CDS             1899..>2137
                     /locus_tag="VNT12_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45048.1"
                     /translation="MRFDLPDAYWKKDVALFFSRRFALILKMLYPMAIIVPVAASGMP
                     AKYAAAVIGIVAVMAGTFGAGESLTIDLNDGILVRV"
BASE COUNT          370 a          673 c          682 g          412 t
ORIGIN      
        1 atgtcctcct caccgtgccc ggcttctata gctttgtcga agcgggccac aacggcctcg
       61 gcgatccgca ttcgcaggtc gtgggcttct gcggcgtcga ggacgagacc cgcgtccttg
      121 cgggcgagtc tggccgagaa gctggtcggg aagtcttctt cgaccatcat ggtgcctttc
      181 atctgggcgt agggtaggct gagcgggccg ccctcgatgg tctcgaggaa cttcgcaggg
      241 tcaacgccga tcgcctccgc gaaagcgacg gtctcggcca gcacaccgag tagacctacg
      301 atccagttgt tgaccacgag cttgagacgg ctcccctcac cagcctcacc tagccacacg
      361 gtcttgcttc ctacggcgct gaagacctgc tcgctccgct ccctgacctt ctcaggccct
      421 gaggcgagca ctacgagctt gccctgctcc gcgggctgct tcgtcccgag gaccggcgcg
      481 tccacgtagg cgacgccact gtcccccgcc agcccggcga gacgctcgtt gccgccctcc
      541 cccaccgtac tcatctgcag ccacacacca tcttcggcca gcgcagagag cacgtctttc
      601 ccgacggttt cctccacaac gtctgcgtca gggagcatgg tcaggatgaa gtctgcgcct
      661 cgagcagcgt ccccgggatt gtcggcgacc tccgcgcctt ccttctccag cggctctgcc
      721 ttctcgcgcg agcggttcca ggcgcgcacc tccatgccag ccgagaggag gttgcgagcc
      781 atggcggcgc ccatgatgcc ggtccctaag actgctaccg tcgtatggtc agccattggc
      841 cctcctgtct atgaatcgtc cgtacttacc atactccact acagcgcaaa tacgggtgat
      901 gcgtctgaca accgtcctgc ggggagggcg ctacaattct cgtgccatga gatccaacac
      961 acagaacgcc cagccggccc tggaagcgaa gaacattagc cgcctctaca agagcagcgg
     1021 gcgcggcgtc tctgacgtaa ctttgcgcct agagcgcggt gaggtctacg gtctcatggg
     1081 accgaacggc tcgggtaaga gcacactcct gcgcgtcctc tccacggcca ttgctcctga
     1141 ctccgggggt ttctgggtga acggggtggg cggcctgaaa gagaagcggg aggtccgcgc
     1201 ccgcatgggt ctgatggtcg accgtccgac gcactacggc gacctcacgg gcctggcgaa
     1261 cgcgtacttc ttcgcccgcg cctacgggat ggaggaagcc aaagccgagg ccgcgctcgc
     1321 ggagctcttc gaatacttcg acctggctga gtatgcggat gacagggtaa agacctattc
     1381 gtacgggatg ggcaagaagc tcgcgctgat cgaggcgctg gcgcacggac cagaggtgct
     1441 gctgttggat gagccctcgc tagggctcga ctatacctcg cagctcgcct atcagagcag
     1501 gatcagggac ctcgcaggct cgggcgtcgc tgttctgctc gcgagcaacc aggtcgatga
     1561 ggtcgaggcc ctgtgcgaca gggcagcctt tttgcaccgt ggagaggtcg tcgccgaggg
     1621 aacgccggag gatctcgtat cccgtgtcgt aggcgtcagg cgcatcgtgg tgacgttgcg
     1681 caatcccgtg gagtacggtc gtgtagctga ggtcgtcggt gtggagcgtg tcgttccgga
     1741 gggaagggat cttgtcttcc actgcgagga acggccaggc atagtcgccg acgtcgtggc
     1801 cggcatcgtc cgctctggcg gggacatcgt gaacctgtct gtgcagaggc ccaacttgga
     1861 ggacgtgttc gtagagctga ctggggaggc gctcagagat gaggtttgat ctccccgacg
     1921 cctactggaa gaaggacgtc gcgctgttct tctccaggag gttcgctctg atactgaaga
     1981 tgctctaccc catggccata atcgtgcccg ttgcggcgag cggcatgcca gcgaagtacg
     2041 cggcagccgt cataggcatt gtcgcggtga tggcagggac cttcggggcc ggtgagagtc
     2101 tgaccataga cttaaacgat ggcatcctgg tgcgcgt
//
LOCUS       DATMJA010000117         3705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_667342, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000117 DATMJA010000000
VERSION     DATMJA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667342"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VNT12_02670"
     CDS             <1..249
                     /locus_tag="VNT12_02670"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HWI45049.1"
                     /translation="APSPIKPGQIYEYVIDLWATGITFLPGHRMRVEITSSSHPRWER
                     NLNTGESTLESSRTEVASQAIFHDAGRPSRITLTLVEG"
     gene            254..1102
                     /locus_tag="VNT12_02675"
     CDS             254..1102
                     /locus_tag="VNT12_02675"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HWI45050.1"
                     /translation="MNVSKAVLITGCSSGIGWATAERLADVGWKVYATARNVEKIAPL
                     EQHGCELLPLDVTDEDSMRSAVDEVERQEGAVGVLVNNAGYSQSGAVESVPMEKVRRQ
                     FETNVLGLARMCQLVLPGMRRQGYGRIVNISSMGGKLTFPGAGYYHATKHAVEALSDA
                     LRFEVEGFGVKVSVIEPGLIRTGFADTAVDSMDGSEGENPYAGFDEGVARATTENYER
                     GPFSKLGGGPEVVAEAIEHAVSARSPRSRYAVTPSAHLFLRLRRLLPDRAWDAILRTT
                     YPQPGR"
     gene            1106..1846
                     /locus_tag="VNT12_02680"
     CDS             1106..1846
                     /locus_tag="VNT12_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HWI45051.1"
                     /translation="MRIEENTFLVTGGGSGLGEATASMLATSGARVVVADLKGEAPEG
                     GRFVETDVTDEGSVQAAVNAALEVGDLRGAVNCAGVASAEKVLGREGPHSLDSFAKVV
                     QVNLIGTFNVIRLAAEAMVSSDPSASGERGVIVNTASVAAFDGQIGQVAYAASKGGVV
                     SITLPVARELASKGIRVMTIAPGIFDTPMMAGLPEEARDSLGRQVPFPSRLGRPEEYA
                     NLVKHIIENEMLNGEVIRLDGSIRMAPR"
     gene            complement(2041..2451)
                     /locus_tag="VNT12_02685"
     CDS             complement(2041..2451)
                     /locus_tag="VNT12_02685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWI45052.1"
                     /translation="MNDTVQALEPFVAQWAVLLTTYKQDGTPVGTAVNIVVEGDRAYF
                     RTWDTAWKLRRIRNNPEVQFAPSTARGKPTAPAIGARATVLEGEESAHAGRLLARKYP
                     ALHGLLVPLVHRLRGNKTMHVELTPAKAPSQNGD"
     gene            complement(2448..3236)
                     /locus_tag="VNT12_02690"
     CDS             complement(2448..3236)
                     /locus_tag="VNT12_02690"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWI45053.1"
                     /translation="MNPPQVLVTGGTGVLGRRVVERLCSEGIRARVFSRSGKPGTITG
                     DLLTGEGLRPAVQGIDTIIHCASSPFRKAHQVDVEGTGRLLETAAEAGVSHLAYVSIV
                     GIDLATSYPYYRIKLETERVVERSPVPHTILRATQFYDLVLMAMRFLDRLPVMVVPRG
                     FLGQPIDAGEVAGRLVDLALSGPAGRVPDIGGPEVKTLADAARGYLRAKGSRQSIFEI
                     PVPGKTARAFREGALVCPDQAYGKIRWEDFLRPVMATGREVTER"
     gene            complement(3233..>3705)
                     /locus_tag="VNT12_02695"
     CDS             complement(3233..>3705)
                     /locus_tag="VNT12_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HWI45054.1"
                     /translation="RARPTAARSHAAPGSRWRPRFESSPQQEERLMEGFLQASLGGDM
                     VALLELLSDDVTLYSDGGGKPRAALRPIYGADKVARFLTGILSNIPPDFTVRRTRVNG
                     RPGLVGYFGDGSPQSVVTLEVAEGGIRTIRLVVNPEKLGNVPPLEQAKREESSG"
BASE COUNT          673 a         1147 c         1206 g          679 t
ORIGIN      
        1 gcgccttccc ctatcaagcc cggtcagatc tacgagtacg tcattgacct ttgggcaacg
       61 ggcatcacgt tcctcccagg gcacaggatg cgggtggaga tcacatcgag ttcccatccc
      121 cgctgggagc gcaacctcaa cacgggcgag agcacactag agtcatcccg caccgaggta
      181 gccagccagg ccatctttca tgatgcgggg cgaccgagca ggatcacgct gactttagta
      241 gaagggtaga gagatgaacg tctcgaaagc tgttctgatc accggctgtt cttcaggcat
      301 aggatgggct accgccgagc gcctcgctga cgtgggctgg aaagtctacg ccacagcccg
      361 caatgtcgag aagatagctc cgctcgaaca gcatggttgc gaactcctac ccctcgacgt
      421 gaccgacgag gactcgatgc gctcggcagt cgatgaggtc gaacggcagg agggggccgt
      481 aggcgtactc gtcaacaacg ctggttacag ccagtcaggg gccgtcgaat cggtgcctat
      541 ggagaaggtc cggcggcagt tcgagacaaa cgtcctcggg ctggcgcgga tgtgccagct
      601 cgtgttgcca gggatgcggc ggcaggggta cggcaggatc gtcaacatct cctccatggg
      661 cgggaagctg acgttccccg gcgccggcta ctaccacgcg accaagcacg cggtcgaggc
      721 gcttagtgac gccctgcgct tcgaagtcga aggcttcggc gtcaaggtct ccgtcataga
      781 gccaggtctc atcaggaccg gcttcgcaga caccgccgtc gactcgatgg acggctcaga
      841 aggggagaac ccgtacgcag gcttcgatga gggcgtcgcc agagcgacga cggagaacta
      901 cgagcggggt cccttctcga agctgggcgg cggacccgag gtggtagccg aagctataga
      961 gcatgcggtc tcggcccgca gtcccaggag ccgctatgct gtcacacctt cagcgcacct
     1021 gtttctgcgg ctcaggcgtc tcctgccaga cagggcctgg gatgcgatct tgagaacgac
     1081 ctatcctcaa ccggggaggt agaaggtgcg catagaggag aacacgtttc tcgtgaccgg
     1141 cggtggctcc ggtttggggg aagccacagc cagcatgctc gccacgtcgg gagcgcgcgt
     1201 cgtggtcgcc gacctaaagg gcgaggctcc tgaaggcggc cgctttgtcg agaccgacgt
     1261 taccgacgag ggcagcgtgc aggcagccgt gaacgcggcc ctggaggtcg gagatctacg
     1321 cggcgccgta aactgcgccg gggtcgccag cgcggagaag gtcctcggaa gagaagggcc
     1381 gcactcgctc gactcgttcg cgaaggtcgt gcaggtcaac ctcatcggca ccttcaacgt
     1441 catcaggctc gctgccgagg ccatggtcag tagcgaccca tcagcgagcg gagagcgcgg
     1501 cgtcatcgtc aacacggcca gcgtggctgc gttcgacggc cagatcgggc aggtagccta
     1561 cgccgcgtcc aaaggcggcg tggtctcgat aaccctcccc gtcgcgcgcg agctcgccag
     1621 taaaggtatc cgcgttatga ctatcgcccc tggcatcttc gacaccccga tgatggccgg
     1681 tctgccagaa gaagccagag actcactggg aagacaggtc cctttcccct cgcggctcgg
     1741 caggcccgag gagtacgcga acctcgtgaa acacatcatc gagaacgaga tgctcaacgg
     1801 cgaggtcatc aggctcgacg ggtcgatccg gatggcgcct cgctgagagc gctgtgcgga
     1861 aggtttgacc ttccgcacag cacggctaca ggtatcaggc ttcaggattt tcctttctgc
     1921 agagctggac cggaaggcga tgtgtaccac ttgatgttca taggttcgaa aagtgctgtg
     1981 agcgcgtatc ggggtagccc agacggaagg gtgtcctgta gcggcgagga tctgcgcctc
     2041 tcagtctcca ttctgcgatg gggctttcgc gggtgtcagc tcgacgtgca tggtcttgtt
     2101 gccccgcagg cgatggacca gcgggacaag taggccgtgc agggcaggat acttgcgggc
     2161 cagcagcctt ccggcgtggg ccgactcttc gccttcgagc accgtggcgc gagcccctat
     2221 cgctggcgcc gtcggtttgc cacgcgctgt ggaaggggcg aactggactt cggggttgtt
     2281 gcggatgcgc ctcagcttcc acgccgtgtc ccaggtacgg aagtaggcgc gatccccctc
     2341 caccacgatg ttgacagcag tgccgaccgg cgtgccgtct tgcttgtacg tggtcagcaa
     2401 gactgcccac tgcgcgacga agggttctag agcttgcacc gtatcattca tcgctcggtt
     2461 acctcacgac ctgtcgccat caccgggcgc agaaagtctt cccagcggat ctttccgtaa
     2521 gcctgatccg ggcacaccag cgctccttcg cggaacgcgc gcgccgtctt gcccggtacg
     2581 ggaatttcga agatactctg cctgcttccc ttcgcccgta agtagccgcg ggcggcgtcg
     2641 gccagggtct ttacctcggg tccgccgatg tcggggacgc gtcctgcagg cccggagagg
     2701 gcgagatcta ccaggcggcc tgcgacctcc cctgcgtcta taggctgacc gaggaaacct
     2761 ctcgggacaa ccattacggg cagccggtcg aggaaccgca tggccataag aacgaggtcg
     2821 tagaactgcg tagcccgcag gatggtgtgc gggaccggag acctttcgac tacccgctcg
     2881 gtctcgagct ttatccggta gtagggatat gacgtggcca gatctattcc gacgatggag
     2941 acgtaggcga ggtgcgagac acctgcctcc gctgcggttt cgagcaggcg tcccgttccc
     3001 tcgacgtcta cctggtgggc cttgcgaaaa gggctagacg cacagtggat tatggtatcg
     3061 atgccctgta cggccggcct caacccctct cccgtgagaa ggtctcctgt tatggtgcca
     3121 ggcttaccac tccggctaaa gacccgtgcc ctgatgccct cggagcagag acgctctacc
     3181 acacgccggc cgagcacccc cgtccctcca gtgacgagga cttgcggcgg gttcatcccg
     3241 aactttcctc tcttttggcc tgttcgagcg gtggcacgtt cccgagcttc tcggggttga
     3301 cgacaagcct gatggtcctg atgcccccct cggccacctc gagggtcacg acgctctggg
     3361 gactgccgtc cccgaagtag ccgacgaggc caggccttcc gttgacgcgc gttcgcctta
     3421 cggtgaagtc gggcgggatg ttgcttagaa ttccggtgag gaaccgggcg accttgtcgg
     3481 cgccgtagat aggtctcagc gcggcgcggg gcttccctcc gccgtctgag tagagggtta
     3541 cgtcgtcgga gagcagctcg agcagggcaa ccatgtcccc gccgaggctt gcctgcagga
     3601 agccctccat caggcgttcc tcctgctgcg gggagctctc gaagcggggc cgccaccgac
     3661 tgcctggcgc ggcgtgagat ctggcggcag ttggcctcgc tcttt
//
LOCUS       DATMJA010000118         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_667487, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000118 DATMJA010000000
VERSION     DATMJA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667487"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VNT12_02700"
     CDS             <1..401
                     /locus_tag="VNT12_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWI45055.1"
                     /translation="FIPYLGALISIVIPMLLALISDPFSVVWVILAFVIIQQIEGNLL
                     QPIIMSRAVDLHPALVVFAILVMGTLFGIVGVFLAVPLVAALQVLVRELWVQRMDQIG
                     TDPNPPAREPPKRKLPRPISKLLRTLRFSR"
     gene            453..1832
                     /locus_tag="VNT12_02705"
     CDS             453..1832
                     /locus_tag="VNT12_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWI45056.1"
                     /translation="METVRSWTDRDLRRLDEIIVEGERRFKERQPRSGALIGEARGVL
                     AGGATSSWQIARPQMIWVSHGEGSRIYDADGGEYVDLHGGYGVGAVGHGHPKIVEALT
                     RRVPRGTHFAQPTEDAIVVAGELARRFGLPLWRFSNSGTEATMDAVHLMRAITGRDLI
                     VKVEGCYHGHHDSVMVSVSNGKDEIGLAERPYSAPSGTGIPRAITDLTLIVPYNDLDA
                     LERVFEEHRGQIAGMILEPIMMNAGVIPPEEGYLESVREIVDDHGALLAFDEVKCGVT
                     TAYGGASGYLGVSPDMVCLAKAIGGGVPCGAIGGTEEVMGRIADGTYDQVGTFNGNPL
                     TMAASRATLTEILTPEAYEHFDRLGKIMSEGVREVVEQYDLAAYPLAFGGKGSVTFSG
                     RKVRNYRDFLQLDDRFSQAHWLFQHNGGVFLPPWGKAEQWTLSVQHSEVDARRFVDNF
                     ETFARALRK"
     gene            1829..2995
                     /locus_tag="VNT12_02710"
     CDS             1829..2995
                     /locus_tag="VNT12_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI45057.1"
                     /translation="MNAEEGHAGGHVQLVDLAKRFGDFEAVCGIDLDIPPGEFFSLLG
                     PSGCGKTTTLRMIAGFERPSGGHILLDGKDVSGTPPNRRNVNTVFQSYALFSHLTVAE
                     NVAFGLRFAKASREEVRSRVGEALSLVRMQEFRDRKPHQLSGGQQQRVALARALILNP
                     SVLLLDEPLGALDAKLRKALQIELKALQEQVGITFVYVTHDQEEALTMADRLAVMNEG
                     RIEQVGSPREVYEEPASAYVADFLGVSNMLDAQAIGTDSGGRCRVRVGDFELLASQGH
                     TSARGSVKVVVRPERVRVEAPGETGENRLPGKIERVVYAGAISQLVVTLNSGTPIRCM
                     LANDGAVSSFDRGTLVSLYLPCEALRVLRTETTAPNEVKQAISSQLSAVSSKKS"
BASE COUNT          592 a          997 c         1020 g          585 t
ORIGIN      
        1 cgttcatccc ctacctgggc gcgctcatct cgatcgttat ccccatgctt ctggccctga
       61 tctcggaccc tttctcggtc gtctgggtga tcctggcttt tgtaatcatc cagcagatcg
      121 aaggcaacct cctccagccg atcataatga gccgggccgt cgacctgcat cccgccctgg
      181 tagtcttcgc catcctggta atgggcacgc tcttcggcat cgtaggggtc ttcctggccg
      241 ttccactcgt ggccgctctg caagtcctgg tgcgcgagct ctgggtgcag agaatggacc
      301 agatagggac cgaccccaac ccaccagcca gggaaccccc gaagcgtaag cttccacgtc
      361 cgatcagcaa actccttaga accctgcgat tctcgcgttg acataaacaa gccatacccc
      421 tattattgaa tcgtcgttta aaggaggagt gtatggagac tgtgcggagt tggaccgatc
      481 gtgacctccg caggctcgac gagatcatcg tcgagggtga acgtcgattc aaggaacgcc
      541 agcccaggtc gggggctttg atcggggagg cgcggggggt tctggccgga ggggcgacct
      601 cgagctggca gatcgcccgt ccgcagatga tctgggtcag ccacggggag ggctccagga
      661 tctacgacgc cgacgggggg gagtatgtgg atctccatgg cgggtatggc gtcggggccg
      721 tcggccacgg ccatccgaag atcgtcgagg cgttgacacg gagggttccg cggggcacgc
      781 atttcgccca acccacggag gatgctatcg tcgtggctgg cgagctcgcg cggcgtttcg
      841 gtcttccgct gtggcgcttc tccaactccg gcaccgaggc gacgatggat gcggtgcacc
      901 tcatgcgcgc gatcacgggc cgggatctga tcgtcaaggt agaaggctgc taccacgggc
      961 atcacgactc ggttatggtc tccgtgtcga acgggaagga tgagatcggg ctcgccgaac
     1021 gtccctacag cgcgccttct gggacgggca tcccgcgggc tatcaccgac cttaccctta
     1081 tcgtccctta caacgatctc gacgccctgg agcgggtctt cgaggagcat cgtggacaga
     1141 tagcgggcat gatcctcgag ccgataatga tgaacgcggg cgtcatacct cctgaggagg
     1201 gttacctgga aagcgtgcgc gagatagtgg acgaccacgg cgccctgctc gccttcgacg
     1261 aggtcaagtg cggagttact acggcttacg ggggggccag cgggtacctc ggcgtaagcc
     1321 ctgacatggt gtgcctggcc aaggccatag gcggtggcgt gccctgcggg gccatcggcg
     1381 ggaccgagga ggtcatgggc cgcatcgccg acggtaccta cgatcaggtc ggcaccttca
     1441 acggcaaccc gctcaccatg gccgcctccc gcgccacgct gaccgagatc ctcacccccg
     1501 aggcatacga gcacttcgac cggctcggca agatcatgtc cgagggcgtg cgggaggtcg
     1561 tcgagcagta cgacctcgcc gcctacccgc tggcgttcgg gggcaagggg tccgtaacgt
     1621 tctccggccg caaggtccga aactacaggg acttcctgca gctcgacgac cgtttcagcc
     1681 aggcgcactg gcttttccag cacaacggcg gcgtcttttt accgccgtgg ggtaaggcgg
     1741 agcagtggac gctctcggtg cagcactccg aggtcgacgc cagacgtttc gtggataact
     1801 tcgagacctt cgcgcgggcg ttacgcaagt gaacgcggaa gaaggccacg cggggggaca
     1861 tgtacagctc gtagatctcg ccaagcgctt cggcgacttc gaggccgtgt gcggcataga
     1921 cctggatatc ccgccgggag agttcttctc gctgctaggt ccgagcgggt gcggcaagac
     1981 cacgacactg cgcatgatcg ccggtttcga gcgtcctagc gggggacata tcttgcttga
     2041 cggcaaggac gtgtcgggca cccccccgaa ccggcgcaat gtcaacaccg ttttccagag
     2101 ctatgccctc ttctcccatc tgacggtcgc cgagaacgtc gccttcgggt tgcgtttcgc
     2161 gaaggcgtcc agggaggagg tccgtagcag ggtcggagag gcgctctccc tcgtgcgaat
     2221 gcaggagttc cgcgaccgaa agccgcacca gctctcaggc ggccagcagc agcgggtcgc
     2281 gctcgcgcgg gctttgatcc tcaacccgtc cgttctgctg ctcgacgagc cgctcggtgc
     2341 attggatgcc aagctcagga aagctctcca gatcgagctt aaggccttgc aggaacaagt
     2401 agggatcacc ttcgtctacg tcacgcacga tcaagaagag gccctcacca tggcggaccg
     2461 cctggctgta atgaacgagg gcaggatcga gcaggtcggc tcaccccgcg aggtctacga
     2521 agaaccagcg tccgcctacg tcgccgactt ccttggcgtc tcgaacatgc tggacgcgca
     2581 ggccatcggt acggattccg gcggcaggtg ccgggtcagg gtgggagact tcgagctgct
     2641 cgcctcccaa ggtcacacct cggccagggg ttccgtcaaa gtcgtggtgc gtcccgagcg
     2701 cgtgcgggtg gaggccccag gagagaccgg cgagaaccga ttgccgggga agatcgaacg
     2761 cgtcgtctac gccggcgcca tctcccaact cgtggtcacc ctcaacagcg gaaccccgat
     2821 ccgatgcatg ctggccaacg acggggcagt atcgtccttc gaccgtggga cactagtgtc
     2881 gctgtacctg ccatgcgaag ccctgcgcgt cctacgcacc gaaaccaccg cgccgaacga
     2941 ggtgaaacaa gctatcagca gtcagctttc agcggtcagc tcgaagaaat cctgaagcct
     3001 gttgcctgaa gccgcggttc gtatgtgggc ggttcgcgaa ccgcccctac agatccaacc
     3061 tggcaaagtc ttccaccgcg gagccttctg cgccttcctt tttgcgggaa cggcccatca
     3121 ccaggatggc gatcgtgata gctagcatgg aagcgaagag gagtatgctc gccagtgcgt
     3181 tcagcgccgg gatc
//
LOCUS       DATMJA010000119         6667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_667520, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000119 DATMJA010000000
VERSION     DATMJA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667520"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VNT12_02715"
     CDS             complement(<1..1129)
                     /locus_tag="VNT12_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI45058.1"
                     /translation="MRLAPLAPASLLKYRSGDLLARIVKDLEELQNVYLRSFSPLAVA
                     LAVCALASLVLVTFDATMAFVALGFLAATGVGVPSLVRALSKGLGRRQLRLRAELDAR
                     IVDDVQGVQDILAFGREGRERREISAINSKLGRVQRRMALVTGLQNAIGDLMMNLALV
                     AVLIFAIPLVKAGEMDGVFLAFVALVVLGSFEAVAPLGAAFQVLGRSVGAGERLFEIT
                     DSEPWVTDPPEPHSLPQDGTLALDRVCFRYEEDGPPALRDVSFVLRPGSRVAVVGPSG
                     SGKSTLVGLILRFWDPDSGEVRLGGRDVQEYAQEDLRSRIGVVSQNTHVFNDTVRNNL
                     LLADPKADDASLHRAIRKAHLADLVEGLPGGLDGYVGEQGQR"
     gene            complement(1148..3136)
                     /gene="cydD"
                     /locus_tag="VNT12_02720"
     CDS             complement(1148..3136)
                     /gene="cydD"
                     /locus_tag="VNT12_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HWI45059.1"
                     /translation="MDRGLLRQIPPARAYLVASVVVGLLVIVVTVAQMAFLSEIVDRV
                     FLNGGDREGTVTLLLLLLGAAALRAGLLWVREMTAQQGAIRVKNELRERLFAHVLRLG
                     PVYARSESSGELATTLTEGVERLDPYFARYLPQVLLSVVVPLLVVAYILPRDAASAVL
                     LVVTAPVIPVMMVLVGSYAEEHTRRQWLALSRMGAHFLDALQGLPTLKAFGRVAAEEE
                     KVERVSGEFRERTLKVLRYAFLSGLVLEFMTAAAIALVAVVLGVRLINGAISFQDAFL
                     VLLLTPEFYRPLRELGIHRHAGMEGKAAAERIFEILDTPVSPDGSSQENPCGELELSF
                     SGVRFTYPGRDLPALDGLSLNLPAGSRTAVVGRSGSGKSTLVGLLLRFLHPDAGVISA
                     NGVPIGDLPAGAWREHVALVPQRPHLFYGNVLENIRLAKPNATRSEVERAAELAGAAE
                     FIRRMPHGYDTQIEERGQRLSGGEAQRLAIARAFLKDAALLVMDEPTSSLDPESERLV
                     RVALDRLARGRTSPDRRPPPEHRQHRGQGRRPARRPGCGDRDPRRTTRTQRALLALGG
                     RLRGRAGLRVFLRLLGFMRPYWWRVLLGVLLGVATVASNVGLLATAAYVISAAAVVPF
                     LAMLTVPVYLVRHFSVSRAASRYAERLVSHDVTLRLLA"
     gene            complement(3180..3254)
                     /locus_tag="VNT12_02725"
     CDS             complement(3180..3254)
                     /locus_tag="VNT12_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWI45060.1"
                     /translation="MDSVSDSVVHHAHCPVLVVREEGE"
     gene            3583..3918
                     /locus_tag="VNT12_02730"
     CDS             3583..3918
                     /locus_tag="VNT12_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI45061.1"
                     /translation="MDLAGTAMAAGGRTGVIWTLLESDDLNANLVRLEAGGSVGEHIN
                     DEVDVIFVGVSGTGSVLTNGEERRLSAGTLVFVRRGARRSILSRSEGFSYLTIHRRRG
                     PLQIEREAP"
     gene            complement(3973..4173)
                     /locus_tag="VNT12_02735"
     CDS             complement(3973..4173)
                     /locus_tag="VNT12_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45062.1"
                     /translation="MWWADLLWGFWNGLTAWIVLVVHVFGGWEQFPFYNEARSGNWYD
                     FGFLLGAGSPLLGAVGGRGRRG"
     gene            complement(4208..4450)
                     /locus_tag="VNT12_02740"
     CDS             complement(4208..4450)
                     /locus_tag="VNT12_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45063.1"
                     /translation="MARKKWLKGRAGWLGLHVTPLPFTGQWDDQEEHCVVLDIEGAEY
                     NVDLSEKLCHRAVVRLPQEMSRRRSSGSWKRWSSSL"
     gene            4591..5688
                     /locus_tag="VNT12_02745"
     CDS             4591..5688
                     /locus_tag="VNT12_02745"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HWI45064.1"
                     /translation="MAVRDYEAIFAGGGLAAMLLLRELGAALSGRVAVVDPCPPPERP
                     TVHWSYWSHGPTLYDRFSVASWQNARVAQMPAESVAPYTLHLVRSTDVFAHIAEELRT
                     APIEWLHAPAHSVERREDGTYEVGTDAGAVRTGWVFDSVPDIEPAFPSPLRPRAVLSG
                     TGVRVTADRPVFDAAIATLFDPLDERSFAYLLPLSPAEALLESATFDSAIQEEDQTPL
                     LRYLRDRYPGATFEVTHVEHGHIPLGFAPSRTAGPRHVLLGAKRGLVKPSAGYGVVRI
                     ALESKRLAGLWKQGLPLPPSQRSSLRWLLLDLGFLQLAANDPRRPLALVRNVMHAVPL
                     VQSLRFIDEELPPRQLATVFGSALPAVLGKP"
     gene            6042..>6667
                     /locus_tag="VNT12_02750"
     CDS             6042..>6667
                     /locus_tag="VNT12_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI45065.1"
                     /translation="MSRKDFLRLGGAGLIGAMLLDAAGCNSSHGKVVRFLTGTRETAA
                     LERAVTELHVDRFEEQHPNIELQRETLQPDAVRRQIQSRLRSKEPPDVFAYDTGPGFG
                     GVLADAGLLRPLENAYKKHAWDIYDWAKQQATYDGTVYGVPDQVEEIIVYYNKDLIPE
                     VPHTVEELRQISGELKGRGKIPLAFGNRAQYPASHLFSIATSNVLGREG"
BASE COUNT         1268 a         2204 c         2085 g         1110 t
ORIGIN      
        1 gccgctggcc ctgctcaccg acgtacccgt ccagcccgcc tggcagccct tccacgagat
       61 ccgccagatg cgcctttctt attgcccgat gcaaagaagc gtcgtctgcc ttcgggtcgg
      121 ccagaagcag attgttgcgc accgtgtcgt tgaagacgtg ggtattctgg gagacgaccc
      181 cgatcctgga tctcagatcc tcctgggcgt actcctggac gtcccgtccg ccaagacgga
      241 cctcgccgct atcagggtcc cagaaacgca ggatcaagcc taccagcgtg ctcttgcccg
      301 agccgctggg tcccactacc gccacacggc tgccgggcct caatacgaac gagacgtccc
      361 gcagcgctgg cggaccgtct tcttcgtagc ggaaacagac gcggtccaac gccagtgtgc
      421 cgtcctgtgg cagggaatgg ggctcgggcg ggtccgtgac ccagggttct gagtcggtga
      481 tctcgaagag gcgttcgccg gctccgacgg aacggccgag gacctggaag gcagcgccga
      541 gcggcgctac ggcctcgaag ctgccgagca ctacgagcgc gacgaacgcc agaaagacac
      601 cgtccatctc ccccgccttg acgaggggta tcgcgaagat aagcaccgcc accagcgcca
      661 ggttcatcat caagtcgccg atggcgttct gcagaccggt caccagagcc atccggcgct
      721 gcacccggcc gagcttacta tttatggcag agatctcgcg ccgttccctg ccttcccgtc
      781 cgaaagcgag gatgtcctgc accccctgga cgtcgtccac aatacgggcg tcgagctccg
      841 cacgcagccg cagctgccgc cgtcccaacc ctttcgagag cgcccgcacc agagacggta
      901 ccccgacgcc ggtggccgcc aggaagccaa gggccacgaa agccatcgtg gcgtcgaagg
      961 tgacgaggac gagagaggcg agagcgcaga cggctagcgc cacggccaag ggggagaagg
     1021 agcgcaggta gacgttttgc agctcctcga gatccttcac gatgcgggcg aggaggtccc
     1081 cgctgcggta cttcagcagg ctggccggcg cgaggggcgc gaggcggatg aagaagcggg
     1141 ttctgagtta cgccagcagc ctcaaggtaa cgtcgtgcga gacgagcctt tcggcgtaac
     1201 gcgaggcggc ccgcgagacg ctgaagtgcc gcacgaggta gaccgggacg gtgagcatgg
     1261 ccaggaaagg cactacggcg gcggcggaga tcacgtacgc cgccgtagcc aggagaccca
     1321 cgttgctcgc taccgtcgct accccgagca ggacgccaag caggacccgc caccagtagg
     1381 gcctcatgaa accaagaaga cgcaggaaca ccctcaagcc ggcacgtcct cgtaggcgtc
     1441 cgccaagagc gagtaaagcc ctttgcgttc gagtagtcct gcgtgggtcc cggtctccgc
     1501 aacctggcct tcgtgcagga cgacgaccct gtccgcggtg ctgacggtgt tcaggcggtg
     1561 ggcgacgatc aggggaggtc cggccccgcg ccaggcggtc gagggcaacc ctgacgagac
     1621 gctcgctctc cgggtccagg ctggaggtag gctcgtccat gacgagcagg gcggcgtcct
     1681 tgaggaaggc gcgggctatc gctagcctct gcgcctcgcc gccagagagg cgctggcccc
     1741 gctcctcgat ctgggtatcg tagccgtgcg gcattcgccg gatgaactcc gcagcccccg
     1801 ccaactcggc cgcccgctcg acctcggacc gggtggcatt cggtttcgcc agcctgatgt
     1861 tctcgagcac gttgccgtag aagaggtgcg ggcgctgcgg caccagcgcc acgtgctctc
     1921 gccaggcccc cgccggtaga tcccctatgg gaaccccgtt cgccgagatg actccggcgt
     1981 ccgggtgcag gaacctcagg agcagcccga ccagggtgct cttgccggat ccgctgcgcc
     2041 cgaccacggc cgtgcgggag ccggccggca ggtttaggct gagtccgtcg agcgctggga
     2101 ggtctcgccc cggataggta aaacggacgc cggagaagga gagctccagc tcgccacacg
     2161 ggttctcctg cgaacttccg tccggggaca ccggggtatc gagtatctcg aagatccgtt
     2221 cggcggcggc cttgccctcc atcccggcgt gacggtggat gccgagctcc cgcaggggcc
     2281 ggtagaactc cggtgtcagg aggagcacca ggaaggcgtc ctggaaggag atggcgccgt
     2341 tgatcaggcg cacccccaga accaccgcga cgagcgctat ggcggcggcc gtcatgaact
     2401 cgagcaccaa cccggagagg aacgcgtagc gcagcacctt cagggtacgc tccctgaact
     2461 cgccgctaac ccgctctacc ttctcttcct cggccgcgac gcgtccgaac gctttcaacg
     2521 tcggcagacc ctgcagggcg tcgaggaagt gcgcgcccat gcgcgagagc gcgagccact
     2581 ggcgccgcgt gtgctcctcg gcgtagctcc cgaccaggac catcatcacc ggtatgacag
     2641 gcgcggtaac gaccagcaac accgcgctgg cagcgtcgcg gggaagaatg tacgccacaa
     2701 ccagcagtgg aacaacgacg ctcaggagaa cctgcggcag gtagcgggcg aagtacggat
     2761 cgagccgctc gaccccctcc gtcagggtcg tcgcaagctc gccggaactc tcgctacggg
     2821 cgtagaccgg tcccagccgc agaacgtggg cgaacagcct ctcccgcaac tcattcttta
     2881 cccggatggc accttgctgc gccgtcatct ccctgaccca gagcaggccg gcccgcaggg
     2941 ccgcagcccc caggagcagc aacagcaggg taacggtccc ctcgcggtct ccgccgttca
     3001 gaaagacccg gtccactatc tcgctaagaa aggccatctg ggcgaccgta acgacgatca
     3061 ccaggagccc gactacgacc gatgccacca ggtacgcccg ggcgggcggg atttgccgga
     3121 gcagacctct atccattcag ttacccgtcc gctccgggga ggtacaccgc agcgtcacgc
     3181 tactcacctt cctcgcgtac gacgagcacc gggcaatgcg cgtggtgcac gacggagtcg
     3241 gagacgctgt ccatgagggc ccgttcgagg ccgccttgcc ccagcttccc accacgacca
     3301 tacccccgcc ggtctcctcc gccaggctag gtagagcaaa gctcgcagag catggcgtgc
     3361 ctcgctgctc aactccaggc gcaaccttcc gtcctttcgc tggtttcggt ctcttcattc
     3421 tcgctcatcg tggccccaat ctccaaaata catgctgttt ctgtaataga taatgaatgg
     3481 tgtcgcgcag gcctctagga ggttcactgc accgtccgga ctgcacgcct gcgcaaggag
     3541 gagatcatct atgacgcaca gcccggatag aggggatagc tgatggatct ggccgggacg
     3601 gcaatggctg ccggcgggcg gacgggagtt atctggacct tgctggagag cgacgacctg
     3661 aacgccaacc tggttcgctt ggaggccgga ggaagtgtgg gcgagcacat caacgacgag
     3721 gtggacgtga tcttcgtcgg cgtctcgggc accgggtcgg ttctcacgaa cggtgaggaa
     3781 cggcgcctgt cagccgggac gctggtgttc gtacgcagag gcgcgaggag gtcgatcctc
     3841 tcccgttccg agggcttctc atacctcacg atccaccggc ggcggggtcc gctgcagata
     3901 gagcgggagg caccatgaag caggagaacg tctcgaagtg aagacccact tcgaagacag
     3961 ttacacggtc tgctaacctc gccggcctct cccacccacg gcgcccagga ggggggagcc
     4021 ggctccgagc aggaacccga agtcgtacca gttcccgctc ctcgcctcgt tgtagaatgg
     4081 gaactgctcc caaccgccaa acacgtgcac gactagaacg atccaggctg taaggccgtt
     4141 ccagaagccc cacaataggt ctgcccacca cacgatgttt acctcctctt tgacctacgc
     4201 agcttgatta cagcgatgat gaccaccgct tccacgagcc cgaggacctc ctacggctca
     4261 tctcctgcgg tagccgcacc accgcgcgat ggcacagctt ctcagagaga tcgacattgt
     4321 actccgcgcc ttcaatgtcc aggactacgc aatgctcttc ctggtcatcc cactggccgg
     4381 tgaagggaag aggcgtgacg tgcagcccta gccagccggc tcttcccttc agccacttct
     4441 tcctcgccat gcgcttacct ccttgaagcc ttgtgcgctt tcaattgtat ggtgaagcgg
     4501 acaaggtttt cctgagcagc ctggggcaat tggctgatca agattactca gagttccccc
     4561 tccgcggcgc gcaacgtatc attgtggctt atggccgttc gagactatga agctatcttc
     4621 gctggcggcg gccttgcggc catgttgctc ctgcgcgagc tcggggcggc tctatccgga
     4681 cgggtcgcgg tcgtcgaccc gtgcccgccg ccggagcggc cgaccgtcca ctggagctac
     4741 tggagccacg gtccgaccct ctacgaccgg ttctccgtcg caagctggca gaacgccagg
     4801 gtagcgcaga tgccggcgga gtccgtagcg ccatacaccc tgcacctggt gcgctccacc
     4861 gacgtgttcg cccacatagc cgaagaactg agaaccgcgc ccatcgagtg gctccatgcc
     4921 ccggcccatt cagtcgagcg ccgggaggac ggaacgtacg aggttggcac ggacgccgga
     4981 gcggtgcgta ccggctgggt attcgacagc gtccctgaca tcgagcccgc gtttccgtcc
     5041 cccctccggc ccagggcggt gctgagcggc actggcgtcc gcgttacggc tgaccgtccg
     5101 gtgttcgacg cggcgattgc caccctgttc gacccgctcg acgaacggtc cttcgcctac
     5161 ctgctgccct tgagccctgc tgaagccctg ctcgagtcgg caaccttcga ctcggccatc
     5221 caggaagaag atcaaacgcc gctgctaagg tacctgcggg accggtatcc gggggccacc
     5281 ttcgaggtca ctcacgtgga acacgggcac ataccgctcg ggttcgcgcc ttcgcgaacc
     5341 gccggcccta ggcacgtgtt gctaggcgcc aagcgcggcc tggtcaagcc aagcgccggc
     5401 tacggcgtgg tccgcatagc actggagagc aaacgactgg ccgggctctg gaagcagggc
     5461 ctcccgctgc cgccgagcca gcgctcctcc ctgcgatggc tgctcctcga cctagggttc
     5521 ttgcaactcg ccgcgaacga cccccggcgg ccgctggctt tggtgcgcaa cgtcatgcac
     5581 gccgttcccc tggtccaatc gttgcgcttc atcgacgagg agcttccacc gcgacagctg
     5641 gcgacggtct tcggttccgc gctccctgcc gtgctcggaa aaccgtagca cgcgcggcac
     5701 cctgggttcc gaccctaccc cggtgcggca ataagggcta ctgactgcat cggcgggcct
     5761 tagctctacg gtaacgaaag tatcaccggg ccttgatcaa gatggaccga ctgtctgcat
     5821 tgatcaccga tgtttgtccc ggtaactgtc gagtctccct cgaagatatc ctaaactccc
     5881 ttgctccatg tcgttggctt atcttcgacc gtcggcaaac atgtaagatt gcaacgatat
     5941 agaggcgact catgtgatgg ctgctcacca aacccgagaa ggtttggcga gagccagggt
     6001 agcagggaaa ggaagggcgg acgggaaaat tgatgagggc agtgagccgc aaagatttcc
     6061 tgaggctcgg gggcgctggg ctcatcggtg cgatgcttct ggacgctgca ggctgcaaca
     6121 gcagtcacgg taaggttgtc aggttcttaa ccggcaccag ggaaacggct gccctcgaga
     6181 gggcggtaac ggaattacac gttgacaggt tcgaggagca acacccgaac atcgaattgc
     6241 agcgggagac gctacagccg gatgcggttc gcaggcagat acagagcagg ctgagatcca
     6301 aggagcctcc agacgtcttc gcctacgaca ccggtcctgg cttcggcggt gtgctggccg
     6361 acgcgggact actgcgtccc ctggagaacg cttacaaaaa gcacgcctgg gatatctacg
     6421 actgggcgaa acagcaggcc acctacgacg gtaccgtcta cggcgtccct gaccaggtcg
     6481 aagagataat cgtctactac aacaaggacc tcatacccga ggtgccacac acagtggagg
     6541 agctacgcca aatctctggc gagctcaagg gccggggaaa gatcccgctc gcttttggga
     6601 acagggcaca gtaccccgca agtcatttgt tcagcatcgc gaccagcaac gtgctgggcc
     6661 gggaggg
//
LOCUS       DATMJA010000120         6363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_845591, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000120 DATMJA010000000
VERSION     DATMJA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845591"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VNT12_02755"
     CDS             <1..799
                     /locus_tag="VNT12_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI45066.1"
                     /translation="EVTVVQRGGQLMGREDADVAEAVAEILREDGIEVLLGTQTRRAA
                     QDDTGKIILTVDTSERERTLEGTHLLVAAGRPPNTEILNLDAAGIETDKRGFIKANEC
                     LETSAPGVYALGDVKGGPAFTHISYDDFRIIRTNLLEGGGATVAGRPVPYTMFIDPQL
                     GRIGLTEQEARDRGCDVRVATIPMTYVTRAVEMGETRGFMKAVVDSETDQILGCAVLG
                     IEGGEIMAMIQIAMMGNLPYRVLRDAVFAHPTLAESLNTLFSSVEHR"
     gene            928..2130
                     /locus_tag="VNT12_02760"
     CDS             928..2130
                     /locus_tag="VNT12_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWI45067.1"
                     /translation="MMPEISLVGVLIVAAVAFVVPLTLGLVPALRVPSVVIEIVAGII
                     IGPAVLGLVEVDEPLQFMALVGLAFLLFLAGMEIDLGGLLRGGALRSAGLGFALSLVI
                     AFGTASLLGVAGLIQAPLLVAILLSATSLGIVVPVLADAGQVRSTLGQLIISASSIAD
                     FGAVILLSLFFSGDSSSVGSTLLLIGAFVVLVVSIGITVAEVEHLGRLSMVLRNLQDS
                     SAQIRVRGAFLFLIGFAVLAQLLGLEIILGAFIAGTVLRLVDKDGMMTHPLFRQKLEA
                     IGFGVFIPFFFVTSGIQLDVGALLAGGGAALAPVPIFLFALLLARGLPAILYKPMIST
                     RGVLAAALLQATSLPFIVATTGIGMELGILSPATGAALVVAGLLSVVLFPLGALTLLR
                     GGKRASAE"
     gene            complement(2247..3101)
                     /gene="folD"
                     /locus_tag="VNT12_02765"
     CDS             complement(2247..3101)
                     /gene="folD"
                     /locus_tag="VNT12_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HWI45068.1"
                     /translation="MAKILDGRTAAAEVRSEVASQVAGLKEKGVPVRLDVILAGDDPA
                     SVTYVASKERDSEEVGIESRVHRFSADVTQEELSDLVGRLNEDSAVSGFFIQLPLPEG
                     LDSMPLISSIIPTKDVDGLSPESVGRLVVDLPSLLPCTPHGVIQLLKRNGIELSGCEA
                     VVIGRSNLVGKPLAQLLLRENATVTLCHSRTRDLPEVTRRADILVVAAGKREMVGAEH
                     VGEGAVVVDVGIHRKQEGGLVGDVRFDEVEPRAAWISPVPGGVGPMTRAMLLHNTVMA
                     ARMGSREQ"
     gene            complement(3109..4734)
                     /locus_tag="VNT12_02770"
     CDS             complement(3109..4734)
                     /locus_tag="VNT12_02770"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011447.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HWI45069.1"
                     /translation="MPHVYVTGHKNPDTDTIASAIGYAEYKNLVDPENHYVAARLGEV
                     NAQTGWALKKSGAEKPGLLEHIMLRVKDVMAGDVAVARKNEPLRNVGLAMARRNISQL
                     PIVEDDGSLTGIITERNLARMYVRESRGASTFAESPVSVGAMAEVLEGELLVGEIDRE
                     SSGQLWVISMSVASMGQSMKPGDIVVIGDRPRGQRRAIELGAGVLVVSNGVRPEDEVL
                     KVARERGTAVLLSPLDSYVTSRLIQLSVPCWEVMSENPLTVRPDDLVTEITEQVMEVH
                     YRAAIAVDEKRVPIGVVTRTDLLNPKPRRVLLVDHAEIGQSVAGVEKAEVVEILDHHH
                     IGDIETTSPIPATFDPVGSTATLIMERFKASGLRPEEPTAKMLLAALLSDTVILNSPT
                     TTDRDREVARYLEEMLDLDANEFGTEMFEASSDVSSLSAQEIVGLDAKEYGTSSGNKV
                     SVSQVEIVGTGLLERKDELMDALEDLQGENGYLFSALMVTDIMEGGTQLLCVGDCSYV
                     HQAFDSQAQNGVIDLPGVMSRKKQVAPLLLSVL"
     gene            complement(4782..>6363)
                     /locus_tag="VNT12_02775"
     CDS             complement(4782..>6363)
                     /locus_tag="VNT12_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWI45070.1"
                     /translation="LIMQTVALLELFPEVRERYQTRFKYIHVDEYQDTNRAQYRLVNI
                     LAAGHRNLCVVGDDDQSVYSWRGADIRNILDFERDYPEAKVVKLEQNYRSTQTILDAA
                     NAVVANNASRKAKALWTAGGEGERIRVFAASDEYAEARFVVSEVEGLIEGGASPREIS
                     VFYRTNAQSRALEDVLVREGVPYQVVGGVRFYERAEVKDAMAYLSVISNPDDSVSLER
                     IINVPKRGLGDTSVAKLQDYARRNGISLYGALAEADAVGLTGAAKKACRGVRDLFEGL
                     RVAAKEVPPAELIGVVLDDSGYRKELEAEDTVEAESRLENLEELINAAREYERVELEP
                     TLEGFLQEQALYSDQDSLTSEGGRVTLMTLHNAKGLEFGHVFVVGMEEGTFPHARSLD
                     EHNLEEERRLAYVGITRAKETLTLTHAKLRSSWGEREYRMPSRFLSEIPDQYKSGMVS
                     GFANASGHGGWGVALSGRGGPERSASGRTAYKTGEKVRHTRFGVGEVVEAGVGKVVIR
                     FGTEERTFVPEMAPLSKV"
BASE COUNT         1087 a         2058 c         1928 g         1290 t
ORIGIN      
        1 cgaggtcacg gtcgtgcagc ggggtgggca gttgatgggc cgcgaggacg ccgatgtggc
       61 cgaggcggtg gccgagatcc tgcgcgagga cggtatcgag gtcctgctgg gaacccagac
      121 ccgacgcgct gctcaggacg ataccgggaa gatcattctg acggtagaca catctgaacg
      181 ggagcgaacg ctggaaggca cacacctcct cgtagcagcc ggcaggccgc ccaacaccga
      241 gatcctcaac ctcgatgcgg ccggcataga gacagacaaa cgcggcttta tcaaggcgaa
      301 cgagtgtttg gaaaccagcg cacccggcgt ctacgcactt ggagacgtga aagggggacc
      361 ggccttcacc cacatctcct acgatgactt ccgcatcatc cgaaccaacc tgctggaagg
      421 tggcggcgcc accgtcgcag gccgccctgt cccctacacc atgttcatag atccacaact
      481 gggccggatc gggttgactg agcaggaagc cagggaccgg ggctgtgacg tgcgcgtagc
      541 cacgattccg atgacctacg ttacgcgggc tgtggagatg ggcgagacgc gtggcttcat
      601 gaaggccgtc gttgactccg agaccgacca gatcctgggc tgcgcggtgc tcgggatcga
      661 ggggggcgaa atcatggcga tgatccagat agccatgatg ggcaacttgc catacagggt
      721 cctccgcgat gccgtcttcg cccaccctac actcgctgag tcgctgaaca ctctcttctc
      781 gagcgtggag caccgttgag gagccacggc gtagagtctg gcgttcctcc gtccgcggcg
      841 gcaccgcaca tctgcgaaag cgaggagatg atacagttcc tcgccgagga gaatggcacg
      901 tgtgatacct aagccaggag aactctgatg atgcccgaaa tctccctcgt cggcgtgctg
      961 atcgtcgcgg cggtcgcttt cgtcgtgccg ctcacccttg gtctggtacc ggccctgcgc
     1021 gttccctccg tcgtcatcga gatcgtggcc ggcatcatta tcggtcccgc agtgctcgga
     1081 ctcgtggagg tggacgagcc cctgcagttt atggctctgg tcggcctggc gttcttgttg
     1141 ttcttggcag ggatggagat tgatctcggc ggcctgttgc gcggcggggc gctacgcagc
     1201 gccggtctcg ggttcgcgct atcgctcgtg atcgcgttcg gtaccgcctc gttgctcggt
     1261 gtcgcgggac tcatacaggc tccgctgctc gttgcgatct tactctcagc cacctcgctt
     1321 ggcatcgtcg tccccgtgct cgccgacgcc gggcaggtcc gttctactct gggacagttg
     1381 atcatctccg cctcatcgat cgccgatttc ggcgccgtga tcctgctctc gctcttcttc
     1441 tctggggact cgagtagcgt cgggtcgacg ctcttgctga tcggggcatt cgtcgtgctc
     1501 gtcgtctcca tagggattac ggtcgccgag gtcgaacacc tcggaaggct gagcatggtc
     1561 cttcgaaact tgcaggactc tagcgcccag atccgggtgc gcggcgcctt ccttttcctg
     1621 atcggcttcg ccgtcctggc acagcttctg gggctcgaaa tcatcctcgg ggcgtttatc
     1681 gcaggcacgg tcctgaggct ggtggacaag gacgggatga tgacgcatcc cctcttccga
     1741 cagaaactcg aggcgatcgg cttcggcgtc tttatacctt tcttcttcgt caccagcggt
     1801 atacagctcg acgtgggagc gcttctcgcc ggtggcgggg cggcactcgc cccggtaccg
     1861 atcttcctct ttgctctgct gctcgcccgc ggactgccgg cgatcctcta taagcctatg
     1921 atcagcacca ggggtgtgct ggcggcggcg ctcctgcagg cgacctccct tcctttcatc
     1981 gtcgcgacga caggtatcgg gatggagctc ggcattttga gccctgcgac tggcgcagcc
     2041 cttgtggtgg ccggcctgct ctcggtcgta ctgttcccgc tcggcgccct tactctcctg
     2101 cgaggcggca agagagcatc cgcggagtaa tacgtaatcc agtagatcgg ttgtgatcca
     2161 ccgtgctgaa cgcctgatgc agtggattcc cgcttacgcg ggaatgacga gagaagaggt
     2221 cgtcgaggac gttgtgggct gagtctctac tgttcccttg agcccattcg cgcggccatg
     2281 accgtgttgt ggagcagcat cgcacgggtc atcgggccga cgcctcctgg gacgggggag
     2341 atccaggccg cccttggctc gacctcatcg aagcgtacgt ccccgacgag ccctccttct
     2401 tgtttgcgat ggataccgac atcgacgacg accgcgccct ccccgacgtg ctcggcccct
     2461 accatctcac gcttgccggc ggcgacgacg aggatgtcag ccctccgggt gacctcagga
     2521 agatcccgcg tacgcgagtg gcagagtgtg acggtcgcgt tctcgcgcag gagtagttgt
     2581 gcgagcggct tgccaacgag gttagagcgc ccgatgacga cggcctcgca tccggagagc
     2641 tctatgccgt tacgcttgag gagttggatc acgccgtgcg gggtgcatgg cagaaggctc
     2701 ggcaagtcga cgacgagcct cccaacgctc tcggggctga gaccgtccac gtctttggtg
     2761 gggattatgc tcgagatcag gggcatggag tcgagtcctt cgggcaaggg gagctggata
     2821 aagaaacctg agacagcgga atcttcgttc aaccgcccga cgaggtccga aagctcttcc
     2881 tgggtgacgt cagcggagaa gcggtggact ctcgactcta tcccgacttc ctcgctgtca
     2941 cgctccttgg aggcgacgta ggtgacggag gcagggtcgt cgccggccag gatcacatcg
     3001 agccgcacgg gcacaccctt ttctttaagc ccggcgacct gtgaggcaac ctccgagcgg
     3061 acctcggccg ccgccgtcct gccgtccagt attttcgcca cgagacttct agaggaccga
     3121 gagcagtaag ggggccacct gctttttgcg gctcatcacg cccggcaggt ctataacgcc
     3181 gttctgggcc tgggagtcga aggcctggtg cacgtaggag cagtccccca cgcacaagag
     3241 ctgggtgccg ccctccatga tgtccgtgac catgagcgcc gagaacaggt agccgttctc
     3301 cccttgcagg tcctccaggg cgtccatcag ctcgtctttg cgctcgagga gaccggtacc
     3361 gacgatctcc acctgggaga cgctcacctt gttgccagag ctcgtcccgt actccttcgc
     3421 gtcgaggcca acgatctcct gtgcggagag cgaagatacg tccgaggagg cctcgaacat
     3481 ctccgttccg aactcgttcg cgtcgaggtc gagcatctcc tcgaggtatc gagcgacctc
     3541 gcggtcgcgg tccgtggtgg tcggcgagtt caggatcacc gtgtccgaga gaagggcagc
     3601 aagcaacatc tttgctgttg gctcctcagg cctgaggccg ctcgctttaa agcgttccat
     3661 gataagcgtg gccgtcgagc caacgggatc gaacgtggca gggatgggag aggtcgtctc
     3721 gatgtctccg atatggtggt ggtccaggat ctcgacgacc tcggcctttt cgactcctgc
     3781 cacgctctgc ccgatctcgg cgtggtcgac gaggagcacg cgcctcggtt tggggttgag
     3841 gaggtcggtc ctcgtaacca ccccgatggg caccctcttc tcgtccacgg cgatcgccgc
     3901 cctgtagtgg acctccatca cctgctcggt tatctccgtt acgaggtcgt ctggtctgac
     3961 ggtcaggggg ttctcgctca tgacctccca gcagggaacg gagagctgta tcaggcggct
     4021 cgttacgtaa gaatcgagtg gagagagcag tacggccgtc cccctctccc tggccacctt
     4081 caggacctca tcctcaggac gcaccccgtt ggagacgacg agtacgccgg cgccaagctc
     4141 tatggccctc cgctggcccc tcgggcggtc cccgatcact acgatgtcgc caggcttcat
     4201 ggactgcccc atggaggcca cgctcatcga gatgacccac aactggcccg aggactcacg
     4261 gtctatctcc ccgaccagaa gctcgccttc gagcacctcg gccatcgccc cgaccgagac
     4321 agggctctcg gcgaaagtgg aagccccgcg cgactcccgc acgtacatgc gcgccaggtt
     4381 ccgttccgtg atgatcccgg tgagagagcc gtcatcttcc actattggca gctggctaat
     4441 gttccgtctg gccatagcga ggccgacgtt gcgcagcggt tcgttcttgc gggcgacggc
     4501 cacatccccg gccatgacgt ccttcacgcg cagcatgatg tgttccagca gacccggttt
     4561 ttcggcccca ctcttcttca aagcccaccc tgtctgggcg tttacctcac cgagccgcgc
     4621 cgccacatag tggttctcag ggtcgacgag gttcttgtac tcggcgtacc cgatagccga
     4681 ggcgatggta tctgtatcag ggttcttgtg acccgttacg tagacgtgtg gcatgtccct
     4741 ctcctctaat gcgattcttt gaatccgact cctcgataag gtcaaacctt gcttaacgga
     4801 gccatctcgg gaacgaaggt cctctcctca gtcccgaacc tgatgacgac cttcccgact
     4861 ccggcctcga cgacctcccc gaccccgaat cttgtgtgcc tgaccttctc cccggtcttg
     4921 taggccgtcc ttcctgaagc tgacctctct ggtcctccac gcccggacag agcaacaccc
     4981 catcccccgt ggcccgaggc gttcgcgaaa ccggagacca tgcccgactt gtactggtcc
     5041 gggatctcgg agaggaagcg cgatggcatc ctgtactcac gctcgcccca actggagcgc
     5101 agcttggcgt gcgtgagcgt aagtgtctcc ttggcgcgcg tgatgccgac gtaggcgagt
     5161 ctccgctctt cctcgaggtt gtgttcgtcg agcgaacggg cgtgcgggaa ggtcccctcc
     5221 tccataccga cgacgaagac atgcccgaac tcgagcccct tcgcgttgtg gagcgtcatc
     5281 aacgtaacgc ggccaccctc tgaggtcaga gagtcctgat ccgaatacag ggcctgctcc
     5341 tgcaaaaagc cttcgagcgt cggctcgagc tcgacgcgct cgtactccct tgcggcgttt
     5401 atgagctcct cgagattctc caggcgcgac tcggcctcga cggtgtcctc agcctccagt
     5461 tccttcctgt agccagaatc gtcgaggacg accccgatca actccgccgg cggcacctcc
     5521 tttgcggcca ccctcagccc ctcgaagagg tcgcggacac cccggcacgc cttcttcgcc
     5581 gcacccgtca agccgacagc gtcggcctcc gcgagcgcgc catacaggga gataccgttc
     5641 ctgcgcgcgt aatcctggag ctttgcgacc gaggtgtcac cgaggcccct cttgggcaca
     5701 ttgataatcc gctccaggga gaccgagtcg tcgggattgg agatcaccga gagataggcc
     5761 atcgcatctt tgacctctgc ccgctcgtag aacctaaccc cgccgacgac ctggtaagga
     5821 acgccctcgc gcacgagcac gtcctccagg gccctgctct gggcgttcgt cctgtagaag
     5881 accgagatct cccgcggaga agccccacct tcgatcagcc cctcgacctc ggagacgacg
     5941 aacctggcct ccgcgtactc gtccgaagcg gcgaacaccc tgatccgctc tccctcacct
     6001 ccggcggtcc acagcgcctt cgccttgcgc gaggcattat tggccaccac cgcgttagcc
     6061 gcatcgagaa tagtctgcgt cgaacggtag ttctgctcga gcttgacgac cttggcctcg
     6121 gggtagtccc gctcgaagtc cagaatgttc cgaatgtcgg ctcctcgcca gctatagact
     6181 gactgatcgt cgtcgccgac gacacataag ttacgatgtc ctgctgccag gatattgacc
     6241 aggcggtact gggcgcggtt ggtatcctga tactcgtcca cgtggatgta tttgaagcgg
     6301 gtctggtagc gttcgcggac ctcggggaag agctcgagta gggcgacggt ctgcatgatc
     6361 agg
//
LOCUS       DATMJA010000121         3657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_490568, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000121 DATMJA010000000
VERSION     DATMJA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490568"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1167
                     /locus_tag="VNT12_02780"
     CDS             169..1167
                     /locus_tag="VNT12_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HWI45071.1"
                     /translation="MTIGKLRAKGRLCSVVSRAAKEDPAGCGTPFVSYLGMEVAPPWM
                     DDVSESPRFPDGLRKAIDKAQHAGVIGKFTALFPDPAYSRQGYVRTLYLQKPPGPFAT
                     YEKSEYVVPKSEIVPLVEALADGSDRLSRFERYEEDTSCGRDLLVCTHGSHDACCGKF
                     GYPVYETLRYGNARASEKQLRVWRTSHIGGHRFAPTLIDFPEGRYWGHLEPDALEKLV
                     LRNGPVTELGRFYRGWAGLSGKFEQIAEREIFVREGWEWTRYLKSGRTLVPYENEDRA
                     DVRIEYATPGGDTGAYEATVETSGTVMTLFDSGSCPLQEVKQYRVSRLERVRLGRR"
     gene            1314..3278
                     /locus_tag="VNT12_02785"
     CDS             1314..3278
                     /locus_tag="VNT12_02785"
                     /inference="COORDINATES: protein motif:HMM:NF016099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HWI45072.1"
                     /translation="MADGFDEEEVKILTYVRAEHPDLEGPFLANVHRGRGEILHRLLQ
                     ALVRENVAGISSRVSWRSGVEKGLEIQFSRGRTLLVCVRRTLSFGRFDLCGSVTLRDG
                     SRCEIVEHPARLLDLLSDLPGYHSVVRDLAGEARLARFRLEVRNSAANYALALAGASL
                     RRRELAARTRTSPEWVRGRTAGDEMFSPLAFYEQLVVGGHPLHPGAKLKLGLGVGDVI
                     RHSPELGAHPEVALVAVAKGCYRDVSLDERGLTGVLYGEYQGLRQQVANALWEKGLNA
                     DDYGLIVVHPWQFEHVLTRLYAKAMESGEVVPLGGLRIPTRALMSFRTLAPVQRQGER
                     KHHIKTAVNLQLTGAVRTISPNSAENAASISRMLREIGDREGHFGGRFVVLEERAAAY
                     FDPANGAYPPQERRELAKNLAAILREDPEAHVRQGEVPVTASALLAESPVSGEPVLME
                     VLELFAAGRGIAEPELAAVAFVRRYCEICLPGFLTLMGRYGVSLEGHLQNSIPVFRGG
                     ETVRVLVRDFGGVRILRERLLCQGIRVDLYPGSAVVIEDVEDLRNKIYYPVFQNHLGE
                     LVACLVRRLDLEEQCLWQPIAEVCRGVFRELKGDPAIHEQAACDEAALFRPTLGLKAM
                     TTMRLLGDVTHYTFSEAANALAEAEGSARR"
     gene            3458..>3657
                     /locus_tag="VNT12_02790"
     CDS             3458..>3657
                     /locus_tag="VNT12_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45073.1"
                     /translation="MAGLDLEIHKRSPPVQGVEKLSFDLAEKSSKVLSLAGEGSLIIP
                     VAGSYAFGRLEVEGSILYVSAG"
BASE COUNT          679 a         1064 c         1243 g          671 t
ORIGIN      
        1 tccgagcatc tctcggagcg acgatttctc ggctgggttt acggtgaggc gccagtccga
       61 cattatccat acccaacgac gtacgtactc gctatggtca cgagggtgga cactgaagga
      121 gacttggaag aacatgaacg cccctgtcct atgaaggagg cctagataat gacaatcggc
      181 aagctacggg ccaaaggccg cctgtgctct gtcgtttcga gggccgccaa ggaggaccca
      241 gcgggctgcg gtacaccctt cgtcagctac cttgggatgg aggtcgcgcc gccctggatg
      301 gacgacgtta gcgagtcccc ccgcttcccg gacggcctcc ggaaagccat agacaaggct
      361 cagcacgcgg gcgtaatcgg caagttcacg gccctcttcc cggaccccgc gtactcccgc
      421 caggggtacg tcaggacgtt gtacctgcaa aagccaccgg gtcccttcgc cacctacgaa
      481 aagagcgaat atgtggtacc caagagcgag attgtgccgc tagtcgaggc gcttgcggat
      541 gggtcggaca ggctctcacg cttcgaacgt tatgaagagg acacctcgtg cgggcgagac
      601 ctccttgttt gcacccacgg cagccacgat gcctgctgcg gcaagttcgg ttaccccgtc
      661 tacgagacct tgcgctatgg gaacgcgaga gcctcggaga agcagctcag ggtgtggcgc
      721 acgagccaca tcggcggtca ccgcttcgcg ccgacgctaa tcgacttccc cgagggccgc
      781 tactggggac accttgagcc agatgcactc gagaaactcg ttctgagaaa tggccccgtc
      841 acggagctcg ggcggttcta ccgaggctgg gccggcttga gcggaaagtt cgagcagatc
      901 gctgaacgcg agatcttcgt gcgtgagggg tgggagtgga ctcgctacct aaagagcggt
      961 aggacactcg tgccttacga aaacgaagat cgcgccgatg tacgcattga gtacgcgact
     1021 ccgggcggcg atacgggtgc ctacgaggcc accgtggaga caagcggtac cgtcatgacg
     1081 ctcttcgact cgggttcctg tcccctgcag gaagtgaagc aataccgcgt cagccgcttg
     1141 gagagggtcc gtttgggtcg aagatgaagg gtgccggcga ccaggaggag catcctagcg
     1201 gcgaaaagga gattaccggt cggtgcgagg ggtcaagtaa gatggcggtt ctgagaggtg
     1261 gcggtgatct gtggcagggc gtagaggaaa tgattggcga gacgaggacg atcatggcag
     1321 acggattcga tgaagaggag gtgaaaatcc tcacgtatgt gcgtgccgag cacccggacc
     1381 tcgaaggacc gtttcttgcc aacgtccacc ggggccgtgg ggagatactc caccggctac
     1441 tgcaagcgct ggtgcgggag aatgtggcgg gtatctcgag ccgagtctca tggcgctccg
     1501 gggtagagaa gggcttggag atccagttct ctagagggag aacgctcctg gtgtgcgtgc
     1561 ggcggactct ctcgttcggc aggtttgacc tgtgcgggag cgtgaccttg cgggatggat
     1621 ctcgctgcga gatcgtcgag catccggcta ggctcctaga tctcctctcc gacctgccgg
     1681 ggtaccacag cgtcgtccgg gacctggcgg gagaggcccg gctcgcccgc ttccgcttgg
     1741 aggtgcgaaa cagcgcagcg aactacgccc ttgcgctggc gggggcctcg ctcaggcggc
     1801 gcgagctggc ggcccgaacg cgcacctcgc cagagtgggt acgcgggcgg accgcggggg
     1861 acgagatgtt cagcccgctg gccttctacg agcagttggt agtgggcggg cacccgctcc
     1921 acccgggcgc gaagctcaag ctgggccttg gtgtggggga cgtcatccgg cactccccgg
     1981 agttgggagc gcatccggag gtggcgctcg tagcggtcgc gaaaggatgc tacagggatg
     2041 tttccctgga cgagcgaggc ctgaccggcg tcttgtacgg ggagtatcag ggcctccgcc
     2101 agcaagtcgc gaacgcgctg tgggagaagg ggttaaacgc ggacgactac ggcttgatcg
     2161 tcgtccatcc ctggcagttt gagcatgtgc ttacgaggct gtacgccaag gctatggaga
     2221 gtggtgaggt cgtgccactc gggggcttac gcatccctac gcgagcactc atgtcgttcc
     2281 ggacgctggc gccagtccaa aggcagggtg aacggaaaca ccacatcaag acggccgtga
     2341 atttgcaact taccggcgca gtgcgcacca tttcgccaaa ctccgccgag aacgctgcct
     2401 caatctcgcg gatgctgaga gagataggag atagggaggg gcacttcggg ggccggtttg
     2461 tggtcttgga ggagagggcg gcagcatact tcgacccggc gaacggagcg taccctcctc
     2521 aggagcgcag agagctcgcg aagaacttgg ccgcgatcct gcgcgaagac ccggaggccc
     2581 acgtccgtca gggtgaggtc cccgtgaccg cctccgcgct tctggccgag tctcccgtga
     2641 gcggagaacc ggtattgatg gaggtgcttg agctgttcgc cgccggacgc ggcatcgccg
     2701 agccagagct tgccgccgtc gcctttgtgc ggcgttactg tgagatatgt ttacccgggt
     2761 ttctgacgct catgggtcgg tacggggtaa gcctcgaggg ccatctccag aacagtatcc
     2821 cggtgtttcg gggcggcgag actgtgcgcg tgctggtgcg tgacttcggt ggcgtacgca
     2881 tcctgcgtga gcggctcctc tgccagggga tacgggtgga tctctacccc ggctccgctg
     2941 tcgtgatcga ggatgtggag gacctgcgga acaagatcta ctatcccgtc ttccagaatc
     3001 acctcgggga gctcgtcgcc tgcctagtcc gccgcctcga cctcgaagaa cagtgcctct
     3061 ggcagccgat cgccgaggtt tgccgggggg tctttcgtga gctcaaggga gaccccgcca
     3121 tccacgagca ggcggcctgc gacgaggcgg ccctttttag gcctaccctc ggcctcaagg
     3181 cgatgacgac gatgcgtctg cttggggatg tcacgcacta caccttcagt gaggccgcga
     3241 acgcgttggc cgaggcggaa ggcagcgcac gccggtgagc acgtcgacta gcagttggca
     3301 gcgaaccgcc gaaggttgtc ttgacgctga cgagctccgt gtgctgcgat tcctacggag
     3361 catccacccg gagttggtcg cgccatttct ctacgccatc ccccgggcgc ggcgggccac
     3421 tctgaaccgc ctgacgtcct ccgtgctccg tgagggcgtg gcaggactcg acttggagat
     3481 ccacaaacgg tcgccacccg tccaaggggt tgagaaactc agcttcgacc ttgctgagaa
     3541 atccagcaag gtgctgtctc tcgccgggga agggtcgctg atcattcctg tcgccgggtc
     3601 ttatgccttc ggtcgccttg aggtcgaggg gtcgatacta tacgtaagcg cgggtag
//
LOCUS       DATMJA010000122         5287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_430893, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000122 DATMJA010000000
VERSION     DATMJA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430893"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..388
                     /locus_tag="VNT12_02795"
     CDS             56..388
                     /locus_tag="VNT12_02795"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWI45074.1"
                     /translation="MTLTEERLDAAFSALADPTRRAIFTRLSEGEASVKELAEPFTIS
                     LQAVSKHIRVLQRAGLISQGVDAQRRPCRIETDTADELINWITEQKRRWEYRLDALEA
                     HLEQVQTK"
     gene            431..592
                     /locus_tag="VNT12_02800"
     CDS             431..592
                     /locus_tag="VNT12_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWI45075.1"
                     /translation="MTATITFAVVGDKTEVTIHQVGVPEGFLGPESEAGFAATFDKLE
                     ALLERWTDG"
     gene            649..780
                     /locus_tag="VNT12_02805"
     CDS             649..780
                     /locus_tag="VNT12_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45076.1"
                     /translation="MMVVEARTFDGAIVLLRYVASYGREHFWVVKEKLDRMHREEET"
     gene            777..1088
                     /locus_tag="VNT12_02810"
     CDS             777..1088
                     /locus_tag="VNT12_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45077.1"
                     /translation="MIARTWTGTVRRSDAEEYAEYIRETGFAEYGQTPGNRGAWMLRR
                     DEGERTEFITLSLWESEDAIRSFAGDDIEAAVLYPEDERYLIGGESAVAHYEVVGEVV
                     Q"
     gene            complement(1215..1814)
                     /locus_tag="VNT12_02815"
     CDS             complement(1215..1814)
                     /locus_tag="VNT12_02815"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HWI45078.1"
                     /translation="MIITAAIAGMMGSALFGIVLLALSALEYDFMLGIGWRPVADPAG
                     AWPSGLALGPYGLAQVANFVVSGLLLAFFSLGLHLGATNGRGSPLGPAFLFVAGTAIS
                     LMGFETDPIRRIGPRSLHGLIHDAAYVIFVLAFMAALFSLWRRFEEHSRWRAHARYTL
                     ATGILAVLLLLLPGVAYYAFIITLLAWIEATAIRLWRLS"
     gene            complement(2088..2336)
                     /locus_tag="VNT12_02820"
     CDS             complement(2088..2336)
                     /locus_tag="VNT12_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45079.1"
                     /translation="MAEEQESLRNDLRPPVTSTVGPSESPADNGWMRGWWHAVATRVA
                     VWRLEFRRVGLAEEVRRLELLEGDIEFALRLKRRRSRS"
     gene            2775..3752
                     /locus_tag="VNT12_02825"
     CDS             2775..3752
                     /locus_tag="VNT12_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI45080.1"
                     /translation="MHTVALVVFDRISPFEMAVPCEVFGADRSDMGLPNYRFLVCAAE
                     EGLLRTDAGFGIVAAHGLEGLREAQSVVVPAWRDVEEAPPAKLVEALRRSYGCGARIV
                     SLCSGAFVLAAAGLLDGRRATTHWMYADALADRFPAVEVDPNVLYVDQGQILTSAGTA
                     AGIDLCLHLVRLDHGAEVANAFARRMVVPPHRDGGQAQYVRMPVAESSDEGPLSETLA
                     WASANLGEPLTVADMAHHARMSVRTFARRFREVTGTTPLRWVLNQRILAAQRRLESSE
                     DPVERIAQDCGFGTGATFRLHFGREVGVPPTRYRGTFKQDTARRSAASR"
     gene            complement(3812..>5287)
                     /locus_tag="VNT12_02830"
     CDS             complement(3812..>5287)
                     /locus_tag="VNT12_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWI45081.1"
                     /translation="GRIREAVIVADARTQRIVLWNPAAKNIFGYSISEALKLNIEKLV
                     PEPLKSQHRTGITRYAQTGHGPYIDSRRFVELPALTKSGEEICVELSLSPIGLVDDTN
                     GGRRFVLAIVRDVTERKWAEEALRQNEERFRLLVDGVKDYAIFMLDPDGKVASWNEGA
                     HRINGYRQQEILGRHFSVFYPEEDLERSKPERELEIAQEKGTYEEEGWRVRKDGSRFW
                     ASVLITALWDEAGGLRGFAKVTRDITERKRAEEEIQRLNETLEELKDLVGKLVLGQEE
                     EQRRVAYEVHEGLAQVASAAHLRLEAFSLKDSLDTERSQADLEQALTLIQQTISDARR
                     ITATLRPTVLDDLGLPAAISLEVECLRKEGYRVEYEEGLGDERLPAMAENALFRIVQE
                     ALTNIQKHGQTRKMRIELRRREDEVYLEVRDYGRGFDLETTSPGRELGKTIGIARMRE
                     WALLLGGQLKIHTKPGRGTSVVAKVPLPAHEGAFRSRSEIP"
BASE COUNT          972 a         1651 c         1617 g         1047 t
ORIGIN      
        1 cctacgagcc cgccggtgag ctcactttcg gttccgttga cgaccctcgt agctcgtgac
       61 gcttaccgag gaacggctcg acgccgcctt ctcggctttg gccgatccga cccggcgggc
      121 gatcttcact cgcctctctg agggcgaggc gagtgtaaaa gagctggccg agccgttcac
      181 tatcagcctg caagcggtct ccaaacacat cagggtcctc caaagggcag ggctcatcag
      241 ccaaggggtg gacgcccaac gccggccctg tcggattgag acggacacgg cggacgagct
      301 catcaactgg attacggagc agaaacggcg ctgggaatac cgtctcgacg ctctcgaagc
      361 gcacctcgaa caggtccaaa caaagtaggc gaacggatga gcgcacccga acacaaggac
      421 gatcgacgag atcaccgcga cgataacctt cgccgtggtg ggggacaaga cggaggtgac
      481 gatccaccaa gtcggcgtcc ccgaagggtt cctgggaccc gaatccgaag ccggattcgc
      541 cgccacgttc gacaagctcg aagccctcct cgaacggtgg accgatgggt aaatcgttgg
      601 cgtagagaat tcccaagtcg gtaccgtgac ccttataagg gctctacgat gatggtcgtc
      661 gaagcgcgga cgttcgatgg cgccatcgtc ttgctgcgtt acgtcgcctc ctacgggcgg
      721 gagcacttct gggttgtgaa ggagaagctc gaccggatgc atcgcgagga ggaaacatga
      781 tcgcacgcac ctggacgggc acggtgcgcc gatccgacgc cgaagagtat gccgaataca
      841 tccgagagac gggcttcgcc gagtacggcc agactcctgg gaaccggggt gcgtggatgc
      901 tccggcgcga cgagggtgag cggacagagt tcatcacgct ctcgctgtgg gagtccgagg
      961 atgcgatccg ctcctttgcc ggggacgaca ttgaggcggc ggttctttac cccgaagacg
     1021 agcgttatct aattggcggc gagtcggccg tggctcacta cgaggtcgtc ggcgaggtgg
     1081 tccaatgagc acgcgccggc ctccctaact tcgtgaccgt catgtggtcg tcatcggcgg
     1141 tgcgagcgag atcggacggg gcatcgccga ggccgcactc gctccgcggt gcatggcgaa
     1201 ccatccgtcg aaggctagga gaggcgccaa agacgtatcg cggtggcctc gatccaggcg
     1261 agcagcgtga tgatgaatgc gtagtatgct acgcccggca agaggagcaa taacactgct
     1321 agtatccccg tggccagggt gtagcgggcg tgggctcgcc agcgggaatg ctcctcgaac
     1381 ctccgccaca gcgagaatag ggcggccatg aaggccagga cgaagatcac ataggccgcg
     1441 tcgtggatga ggccgtgcag gctacggggt ccgatcctcc gtatcgggtc ggtctcgaag
     1501 cccattagtg atatcgccgt ccccgccacg aagaggaatg ctggaccgag tggcgagccg
     1561 cgcccgtttg tcgctccgag atgaaggccg agcgagaaga aggctagcag taaacccgag
     1621 acgacgaagt tggctacctg cgcgagcccg tagggaccga gggcgagccc gctgggccaa
     1681 gcgcccgccg ggtccgcgac gggacgccat ccgatcccaa gcatgaagtc gtactcgagt
     1741 gcgctcagcg cgagtaggac gattccgaac agagcggacc ccatcattcc cgctatcgcc
     1801 gccgtgatga tcattcgccg cttcgtttcg atccttgccg aggccggtta tatcttgcgt
     1861 cttcttgccc gggtcgaggc gcgtccacgc acgattttac aggcagcacc tcaggttcat
     1921 accgccggga gcggcagaag gggaatcagc cgggaggcaa gcccagctgg accccggtct
     1981 atgcaagccg tttctagagc tcgcatcact tcattagggc cctcggggcg tccctacaca
     2041 aattgtgcaa tgtcgtcact ccagccatcc ctattgcgct tctccgatta actgcgggat
     2101 cgtctcctct tgagacgcag ggcgaactcg atatccccct ccaggagctc caaccggcgc
     2161 acctcctcgg cgaggcccac ccgccgaaac tccagccgcc agacggcgac tctcgtcgcc
     2221 accgcgtgcc accaccctct catccatccg ttgtccgcag gggactctga tggcccgaca
     2281 gtggaagtga caggtggcct gaggtcgttc ctcagggatt cttgctcctc tgccaagcgc
     2341 atctcctctt gctctaatga cgcccttcgg gccctgaggc gcatcagatc gaactcttgt
     2401 tccagttcga aaaggaatcg gttggcctcc gcgttaacag cttcttggct ttgcctagtt
     2461 gcgaacatca caacctccct gatcactcgg taagcggctc ggtcgaggcc cttggatact
     2521 ggccatcagc gcgagtggta cggatctctt gcccctcttg ggcgaggatg tgctcccgct
     2581 cgataagatt tcgcaagcgc gcctcctccg caccaaagga tcctcgaaga acatactcgc
     2641 gatcatcaca ctagctccgc ctggctctgt cgcttgttgc ctgcatcgga attctgcaat
     2701 cgtcagcaca atccgacaag tggcaggaat gccgatcttc gcgtatttac tgccaaatct
     2761 gctatgatgc actcatgcac accgtggcgc tggtggtctt cgatcggatc tctcccttcg
     2821 agatggctgt gccctgcgag gtatttgggg cagaccgttc ggatatggga ttgcccaact
     2881 accgctttct ggtgtgtgcg gcagaggaag ggttgctgcg cacggacgcg gggttcggaa
     2941 tcgtggcggc acacgggcta gaagggctgc gtgaagctca aagcgtggtg gtgccggcgt
     3001 ggcgcgacgt ggaggaggct cctcctgcga agctggtcga ggccctgcga cgaagctacg
     3061 ggtgcggggc gaggatcgtt tccctgtgct cgggggcgtt tgtcctggct gcggcgggct
     3121 tgctcgacgg caggcgtgcg acgacccact ggatgtacgc cgacgccttg gcagaccggt
     3181 ttccggcggt cgaggtcgat ccgaacgtgc tctacgtcga ccaaggacag atccttacgt
     3241 ctgcgggcac ggcggctggg atcgatctct gcctccacct ggtacgcctg gatcacgggg
     3301 cggaagtggc caacgcgttc gctaggcgca tggttgtgcc accccaccgg gacggggggc
     3361 aggcccagta cgtcaggatg ccggtggcgg agagctccga cgaagggccc ttgagcgaga
     3421 cgctagcgtg ggcgagcgcg aacctgggcg agcccctcac ggtagcggac atggcacacc
     3481 acgcgcgcat gtccgttcgc accttcgcgc gtcgcttccg ggaggttaca ggcaccacgc
     3541 cactaaggtg ggtgctaaat cagaggatcc tggccgccca acgtaggttg gagtcctctg
     3601 aggacccggt ggagcgcata gcgcaagatt gcggtttcgg cacgggtgcg acctttcggt
     3661 tacatttcgg gcgagaggtg ggcgtgcctc cgacccgcta ccgaggcacc ttcaagcagg
     3721 acacagcacg ccgctcggcc gcaagccgat gaggctgctc ataccgtctt gccgtggtcg
     3781 gcgtcgattg gtacggttcg tcctcgtgct gttaaggtat ctctgaacgg cttctaaaag
     3841 cgccctcgtg cgcaggtagg ggcaccttcg ccaccacgga ggtgccccta cctggcttgg
     3901 tgtggatctt caactggccg ccgagcagca gggcccactc tcgcattctg gcaataccga
     3961 tcgtcttacc aagttcccta ccgggggatg tcgtctccaa atcgaacccg cgcccgtagt
     4021 cccggacctc caggtaaacc tcgtcctccc ggcgccgcag ttcgattcgc atcttgcggg
     4081 tttgcccgtg cttttgtatg ttggtcagcg cctcctgcac aatgcgaaag agggcgttct
     4141 ccgccatggc cggcaggcgc tcgtctccga gtccttcttc gtattcgacc cggtaccctt
     4201 ctttacggag gcattcgact tccaaagaga tggcggccgg gagcccgaga tcatcgagca
     4261 ccgtcggcct caaggtcgct gttatcctcc gagcgtctga gatcgtctgc tggatcagcg
     4321 tcagagcctg ttctagatcc gcttggctcc tctcggtgtc cagggagtcc ttaagggaga
     4381 aggcctccaa acgtaggtga gcggcactcg caacctgggc tagcccctcg tgcacttcgt
     4441 aggccactcg acgctgttct tcttcctggc ccaacacgag cttgccgacc agatccttaa
     4501 gctcctcgag ggtttcgttg agccgttgaa tctcttcctc cgcccgcttg cgctcggtga
     4561 tgtcgcgcgt caccttcgcg aagccgcgca agccaccagc ctcgtcccac aaggccgtga
     4621 tcaagacact tgcccagaac ctcgaaccat ccttgcgtac ccgccagccc tcttcctcat
     4681 aggtcccctt ctcctgcgct atctcgagtt ctcgctctgg cttggagcgc tccaaatcct
     4741 cttccggata gaagactgag aagtggcgtc cgagaatctc ttgttgacga tagccattga
     4801 tgcggtgcgc cccttcgttc cagctggcca ccttaccatc aggatcgagc atgaagatcg
     4861 cgtagtcttt cacgccatcc accaagagac gaaaccgttc ctcgttctgc ctcagcgcct
     4921 cctcagccca cttgcgctcg gtaacatccc ggacgatagc gagcacaaat cgcctgcctc
     4981 cattcgtgtc gtcgaccaac cctatggggc tcaaggacag ctctacacag atctcttcgc
     5041 cgcttttcgt cagtgcgggc agttccacga acctgcggga gtcgatgtag ggtccgtgtc
     5101 ccgtctgagc gtagcgagtt atgcccgtcc gatgttggga ctttaggggt tcggggacca
     5161 gcttttcgat gttaagcttg agcgcctcgg agatggagta gccgaagatg tttttggccg
     5221 ccgggttcca gaggactatt cgctgtgttc tggcgtccgc gacgatgacc gcctcccgga
     5281 tcctccc
//
LOCUS       DATMJA010000123         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_667932, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000123 DATMJA010000000
VERSION     DATMJA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667932"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VNT12_02835"
     CDS             complement(<1..351)
                     /locus_tag="VNT12_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWI45082.1"
                     /translation="MPKLEVEGVGTFEVPENKRLVLAIEEDAGVDILHRCGSYARCTT
                     CRIEYLEGEPEKMTRAELEVLEKRNLLGQVRLSCQTLSDHDKKVRVLMTVSSTGLDGP
                     GPHPESHITPEPEWV"
     gene            505..867
                     /locus_tag="VNT12_02840"
     CDS             505..867
                     /locus_tag="VNT12_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HWI45083.1"
                     /translation="MDHRPPSIKVDKPWGRFEQYTHNLPSTVKIITVAPGGTLSRQYH
                     HRRDELWVILDKGAQVQLGEEVLEPETGEKIFIPRGTVHRLSTAGESEVRILEISFGE
                     FDEEDIVRLDDVYGRMVQ"
     gene            889..1821
                     /locus_tag="VNT12_02845"
     CDS             889..1821
                     /locus_tag="VNT12_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HWI45084.1"
                     /translation="MSFRGEEESRRDEHAQASAGHAQTGAERERGRPSRMSLLLLLFA
                     VLLLALLAVAAILLWKSLGESISGTSVARDSIDSAGLEPRVRLANAAGQVRVEGVKGF
                     RSVDYEATRYAMGADPAAAKQRASDVPVDISREDSKIVIETDGGKDTGADYTLRVPTG
                     GSVEVESEAGDVEVSGISGNVIVGAEAGDVMVRDVGGDVKVEAPRGDVTVGDVNTDTG
                     SANLETGVGDVSLEDLILGTLETSVEAGDVTLSGRFSGGGRVSVETGDIIARLPPEDT
                     RDLTLQTRVGSILREPTDGGSNGQGTEIQKQQGS"
     gene            1821..2414
                     /locus_tag="VNT12_02850"
     CDS             1821..2414
                     /locus_tag="VNT12_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HWI45085.1"
                     /translation="MFRRGDVGYGGESPEDFWGALRGVWFDPVRFFRGLDPRGGAIRP
                     AIFASVVLYLDLLLEALLQMLWLREFNYALIYAPFLGLVVAIVLAPLLVAGLAVLVLV
                     ILGGGSLARGGFGPLFRSLGYASGIGFALWIPFAPLLAVPYGAFVATVAVKETMNVSS
                     ARAAAATLIPLGAVILTVLALLGPDEAVGFLINPPGS"
     gene            complement(2465..>2731)
                     /locus_tag="VNT12_02855"
     CDS             complement(2465..>2731)
                     /locus_tag="VNT12_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWI45086.1"
                     /translation="EEGAADRVLGVAENLFGAESVLELPNPSMGAEDFAFFLEKVPGA
                     FIWLGVGEDVSGLHTPQFAFDEEVLPRGSALLAALALEAGQHFS"
BASE COUNT          500 a          875 c          854 g          502 t
ORIGIN      
        1 cacccactcg ggttcgggtg tgatatggga ctcgggatgc ggaccaggac catcgagacc
       61 tgtggagctc acggtcatca atacccgcac cttcttgtcg tggtcactta gagtctggca
      121 cgagagcctg acctgcccga gaaggttgcg cttctcgagc acttcgagtt cggccctggt
      181 catcttctcg ggctcgccct ccagatactc gatgcggcag gtggtgcacc tagcgtaaga
      241 accacagcgg tgcaggatat ctaccccggc atcctcttcg atcgccagaa ccaatctctt
      301 gttctcggga acttcgaagg tccccacgcc ctcgacctct agcttaggca ttcattcctc
      361 cttccagaga cggagcttac attctcgtat ctaaccacgc cggcagacca ggcgcgaatg
      421 ccaggtcgcg ggctttcgag gttacacgtc tccaaaaccg taagtggatc ggctaaactg
      481 gccattcacg aaagggggtc acgcatggat caccgcccgc cttctataaa ggtagacaag
      541 ccctggggca ggttcgagca gtacacgcac aacctgccca gtacggtcaa gatcatcacc
      601 gtcgctcctg gcggcacgct ctcacgccag taccaccacc gccgcgacga gctctgggtc
      661 atactggaca agggagccca ggtacagctc ggcgaagagg tcctcgagcc cgagaccggg
      721 gagaagatat ttatcccccg aggaacggtt cacaggctct ccacggcagg cgaaagcgag
      781 gtgcggatac tggagatctc cttcggcgag ttcgatgagg aggacatcgt caggctcgat
      841 gacgtctacg gtcgtatggt gcaataacgg ttctggttta gatcccgggt gagcttcagg
      901 ggcgaagagg agtcccggcg cgacgagcac gcacaggcgt ccgccgggca tgcacagacc
      961 ggggcggaga gggagcgcgg gcggccttcc cgcatgtccc tgctgctcct gttgttcgcc
     1021 gtgctgctcc ttgcgctgct cgcggtggcc gcgatcctcc tctggaagtc cctcggcgag
     1081 agcatctcag gcaccagcgt cgccagggat tccatagatt cagccggtct cgaaccgcgc
     1141 gtacgactcg ccaacgccgc aggccaggtg cgcgtcgagg gcgtaaaggg attcaggtcc
     1201 gtagattacg aggccacgcg atacgcgatg ggcgccgacc ccgccgccgc caagcagcgg
     1261 gcctccgatg ttcccgtgga catctcccgc gaagactcga agatcgtcat cgagacggac
     1321 ggggggaagg atacgggggc agactatacg ctcagggtgc cgacaggcgg ttccgtcgag
     1381 gtcgagtccg aggctggaga cgtcgaggtc tcgggtatct cgggcaacgt gatcgtcggc
     1441 gccgaagccg gggatgtgat ggtcagggac gttggtggcg acgtcaaggt cgaagcccct
     1501 agaggagatg tcaccgtcgg cgacgtcaac acggataccg gaagtgcgaa tctcgagacc
     1561 ggcgtggggg acgtctcgct cgaagacctg atcctgggca ccctagaaac cagcgtcgag
     1621 gccggggacg ttaccctctc cgggcgtttc tccggcggcg ggcgagtctc cgtggagacc
     1681 ggcgacatta tcgccaggct tcctccggag gatacgaggg accttaccct ccagacgcgc
     1741 gtcggcagca tcttgcgcga gcctacggac ggaggttcga acgggcaggg taccgaaata
     1801 cagaagcagc aagggtcgta gtgttccgca ggggtgacgt gggatacggt ggtgaatcgc
     1861 ctgaagactt ctggggcgcg ctgcgaggag tctggttcga tccggtccgc ttcttcaggg
     1921 ggctcgaccc gcggggagga gcgatccggc cggccatctt cgcctccgtg gtcctctacc
     1981 tggacctgct cctcgaggcc ctactccaga tgctctggct ccgcgagttc aactacgccc
     2041 tgatctacgc tcccttcctc gggctcgtgg tcgcgatcgt gctcgcgccg ctgctcgtcg
     2101 ccggcctggc ggtcctagtt ctggtcatcc tcggaggggg ctctctcgcg cgcggaggct
     2161 tcggccctct cttccgctct ctaggctacg cgagcgggat agggttcgcg ctctggatcc
     2221 ccttcgctcc cctcctcgct gtcccatacg gcgccttcgt ggccaccgtc gccgtaaagg
     2281 agactatgaa cgttagctcg gcccgagccg ccgccgcgac cctcatcccg ctcggggccg
     2341 tcatcctaac ggtgcttgcc ctgctcggtc ccgatgaagc cgtaggcttc cttataaacc
     2401 cacctggtag ctaacgctct agcaggtcag ccagtccttg cgcacacgaa tgctgacata
     2461 ctgactagct gaaatgctga cctgcttcca acgcgagcgc cgcgagcagc gccgatcctc
     2521 gcggcaagac ctcctcgtcg aaggcgaact ggggcgtgtg caaccccgag acatcttccc
     2581 cgacgccgag ccagataaag gcccctggca ccttctccag gaagaacgcg aagtcctcgg
     2641 cgcccataga cgggttgggt agctccagga cggactccgc gccgaagagg ttctcagcta
     2701 cgccaaggac gcggtcagca gcaccttcct c
//
LOCUS       DATMJA010000124         6418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_75809, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000124 DATMJA010000000
VERSION     DATMJA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75809"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VNT12_02860"
     CDS             <1..1049
                     /locus_tag="VNT12_02860"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45087.1"
                     /translation="EANAVATVVESLALSRPEVGFFLRSDGRRSLAFPAAADLRERIS
                     QVHGLSLARSLVALEDPVVWGFVSPLAVSFPTRRYLHVAINGRVVEADSFAPAVAKAY
                     ADLLPKGRHPAAFLRLELGPGEVDVNVHPAKSAVRLRGGRSAYPLVVGTIRSALAQEG
                     EMGGGAPPEEGSDGLTLIGQFAGRCIIAQEGEELIVLDQHGAHERILYERLSENPRAE
                     AIPLPSPVVARLPEDLAPEVSNFEVEMQALGFRFEPFGEGAVRISAVPETVTDPETAL
                     LAALHALAGGEDLAKALACKGSTRFGEDLSTEEMEALLREWSETTFREICPHGRPIVK
                     RLTLVDLLREFGRI"
     gene            complement(1107..1265)
                     /locus_tag="VNT12_02865"
     CDS             complement(1107..1265)
                     /locus_tag="VNT12_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45088.1"
                     /translation="MDGGKGIYVGGTLISILISLALYFSGKRESAIFVGLWAPTILNL
                     GQSLLDED"
     gene            1364..2968
                     /gene="ggt"
                     /locus_tag="VNT12_02870"
     CDS             1364..2968
                     /gene="ggt"
                     /locus_tag="VNT12_02870"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWI45089.1"
                     /translation="MSQKVEAATRRPDPGGRSTVFGTKGAVACEHPSAAVIGLRVLDS
                     GGTAADACVAMAAAMAVLSPMQTGMGGDAFLLFYEAESGRVTGVNGSGRAPATVDVEK
                     HVDLDEMPERGGLTVTVPGAVRLWEDAANRYGNQALARLLEPAWELAENGYPVAEVVA
                     RYWEEHEDVLRTNEAATRTFLPEGRAPVAGEIFSQVDLAATISAVAERGADAFYKGEI
                     AASIARSTQQAGGYLSEEDLAAHESTFFEPISTNYRGIQVYEIPPPGQGVAALEMLNI
                     LEGFELATMEPSSADRIHLEVEAKKLAFEDLHEKIGDPAHSVIPTEELLSKDYAARLR
                     GSISRDAAAVVSPPNPGGDTTYLCAVDAEGNGCSFINSLYRGFGSSIVAQGTGVCLHN
                     RGNSFRLEPGHPNAIAPGKRPMHTLIPGLATSDGSLWAVFGVMGAAMQPQGHPQVLVD
                     LLDYGMNPQEAVEHPRHRHENGVLLIEGRVPEEEIARLRGRGHRIEVREDFMIPAGGA
                     QLIRILDNGVRACGSDPRKDGCALAQ"
     gene            2977..3450
                     /locus_tag="VNT12_02875"
     CDS             2977..3450
                     /locus_tag="VNT12_02875"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HWI45090.1"
                     /translation="MTPGQGPARTVAVAGVVGALYVVLSLLVAPLAYGPIQFRLGEVL
                     KPLVITYPATIPAFAVGVGIVNLFSPQTGGLELILMPIVNLVGGTLCWFVARRIGGVA
                     GTYIASLVFALVITAGVATVLHFTVGLPYLLAFGSVVVSEVVLLLLGNAVLVRRI"
     gene            3594..4001
                     /gene="queF"
                     /locus_tag="VNT12_02880"
     CDS             3594..4001
                     /gene="queF"
                     /locus_tag="VNT12_02880"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWI45091.1"
                     /translation="MGQSREQVLGRDVRGPIGAEGLDAVPWSHGDTDAVVEFTTNELT
                     ALCPITGQPDFYELALTYRPNGSLIESKSLKLYLWSFRDSGIFAEDLAATLLKDLVSA
                     CDPVEMTVDLTQQVRGGLRIRTVVHHPPEIPDS"
     gene            4409..>6418
                     /locus_tag="VNT12_02885"
     CDS             4409..>6418
                     /locus_tag="VNT12_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HWI45092.1"
                     /translation="MSARMKDAADLCLHELFEEQARRTPENPALEDANISLTYSELDR
                     LADRLAAYLRTLGIGQDEPVGVYIERRAEYVVACLAAMKAGGAYLILELAYPPPLLAD
                     VVADASPRIVLTQERYADRLPEGTETFFLDEGWEERLDDVSAVDSRPEIDQDNLAFVS
                     YSSGTTGKPKGIANPHRAPVLSYLWRFGISDYAPGDRVGCNVFFIWEMMRPLLRGATT
                     VVIPDDVIYDPKALIRFLEEYGITETLVTPSLLEAILNSSGPDVGEKLSNLRTLWLNG
                     EVVTRALARRAMELLPDARLLNVYSCSETHEVAAGDLRELVENPESTYCAIGVPMDPE
                     RLYLLDADGERVPEDEAGELFVGGECLARGYVDLPEKTEESFPEDPFSSRSGARMYRT
                     GDRARILSDGSLEILGRVDFMVKIRGYSVELGAVEAAIEEGLAVRSCVVVSEGEEGED
                     KRLVAYIVPDPGDDERYSGWSLDPKTGRSKEIRAILQDGLPHYAVPSVFVELESLPIQ
                     ATSGKVDRSELPPPPPRPTREDAGSFARLPEDAPRSEKEALLARAWEDVLRLDEGEVR
                     PEDDFFDLGGHSLAAAQLSSRVEQGFGVHVSMPLFMEDPTLAGLCDRIGTLQRDGKDI
                     QSQPSEDLRAEAVLDPEIAPQPAPNGTRTLRDADNVFLTGATGFLG"
BASE COUNT         1179 a         2056 c         2036 g         1147 t
ORIGIN      
        1 ccgaggcgaa cgcggtagcg acggtggtcg aatcgctcgc cctgagccgc cccgaggtag
       61 ggttcttcct ccgctctgac gggcggcgct ccctggcctt ccccgcggct gccgacctca
      121 gggagcgcat ctcacaggtc cacggcctct cgctcgcgcg gagcctggtg gcgctcgagg
      181 accccgtggt ttggggtttc gtcagcccgc tcgccgtgag cttcccgacc cgccgctacc
      241 tgcacgtcgc cattaacggc agggtcgtgg aggccgactc cttcgcgcca gcggtcgcca
      301 aggcttacgc tgacctgctg cccaaggggc ggcatcctgc ggcgttcctg cggctcgagc
      361 tcgggcccgg ggaggtggac gtcaacgtgc atcctgccaa gagcgccgtg cgcttgcgcg
      421 gtggacgctc ggcttacccg ctcgtagtgg ggaccatcag gtcggcgctg gcacaggagg
      481 gggagatggg tggcggtgcg ccacctgagg agggcagcga cgggctaacg ctcatcgggc
      541 agttcgcagg aaggtgcatc atcgcccagg aaggcgaaga gctgatcgtc ctggaccagc
      601 acggggcgca cgagcgcatc ctgtacgagc ggctctccga gaacccaagg gcggaggcca
      661 tcccgcttcc gagccccgtc gtcgccaggt tgcccgagga cctcgcgccc gaggtgtcga
      721 acttcgaggt agagatgcaa gcccttgggt tccggttcga gcctttcgga gagggtgctg
      781 tgcggatctc ggccgtgccg gagaccgtca ctgatcccga gaccgcgctc ctcgctgcgc
      841 tccacgcgct cgccggagga gaagacctcg ccaaagcgct ggcctgcaaa ggctccacca
      901 ggttcggcga ggacctctct acagaggaga tggaggcact cttgagagag tggtcggaaa
      961 cgacctttag agagatctgc ccccatggga ggccgatcgt gaaacgtctg acgctagtcg
     1021 atctgctccg cgagttcggg agaatctaag gcggtcttgc ggaatcgacg atccgcaaaa
     1081 ccgccccaaa gcgaagtttg aaagccttag tcctcgtcta gcaggctctg gcccaggttc
     1141 aatatcgtcg gggcccagag tccgacgaag atcgccgact cgcgcttgcc ggagaagtac
     1201 agggctagtg agatgaggat ggagattagg gttccaccca cgtaaattcc tttgccaccg
     1261 tccataggtt acctcctctt ggtttacaag cgccaatata cccgccgccc atttctataa
     1321 actgtaagag cgatcatagg tccctacagg aggtccgcag cacgtgtccc aaaaagtgga
     1381 agccgccaca aggaggcctg atccgggagg ccgctcgacg gtcttcggaa cgaagggggc
     1441 ggtcgcctgc gagcacccga gcgccgccgt tatcggcctc cgggtcctcg atagtggagg
     1501 aacagctgcc gatgcgtgcg tcgccatggc tgccgcgatg gccgttctct caccgatgca
     1561 gactggtatg ggcggcgacg ccttcctgct gttctacgag gccgagagcg ggcgggtaac
     1621 cggggtgaat ggctcaggcc gggccccggc gaccgttgac gtcgagaaac acgtggatct
     1681 agacgagatg ccggagcgtg gcggactcac ggttacggtc ccaggcgctg tcaggctgtg
     1741 ggaggacgcc gcgaaccgct atggcaacca ggccctggcg cgtctgctcg agcccgcctg
     1801 ggagctcgcg gagaatggct atcccgtcgc cgaggtcgta gcccgctatt gggaagagca
     1861 cgaagacgta ctccgcacaa acgaggccgc cacacggacc tttctaccgg aaggcagggc
     1921 acctgttgcg ggagagatct tctcccaggt agaccttgcg gccacgatct cggctgttgc
     1981 cgaacggggc gccgacgctt tctacaaggg cgagatagcg gcgagcatcg cacgctccac
     2041 gcagcaggcc ggcggttacc tctcggaaga ggatctcgca gcccacgagt cgacgttctt
     2101 cgagcctatc tcgaccaact acagaggcat ccaggtctac gagatcccac ctcctggcca
     2161 gggcgtcgcc gccctcgaaa tgcttaacat cctcgaaggg ttcgagctgg ccactatgga
     2221 accttcgagc gccgaccgca tccaccttga ggtcgaggcc aagaagctag ccttcgagga
     2281 cctgcacgaa aagatcggag atcctgcaca ttccgtcata ccgactgagg agttgctctc
     2341 caaagactac gctgcgaggc tgcgcgggtc gatctctcgc gacgccgcag cggtggtctc
     2401 tcctcccaac cccgggggcg ataccaccta tctctgcgcc gtagacgcgg agggcaacgg
     2461 ctgttcgttc ataaacagcc tctacagggg gttcggcagc agtatcgtcg cccaaggaac
     2521 cggcgtctgt ctccacaacc gcggcaactc gttccgcctg gaacctggtc acccgaacgc
     2581 catcgctcca ggtaagcgcc cgatgcacac tctcatcccc ggtctcgcta ccagcgacgg
     2641 atctctatgg gccgtcttcg gcgtgatggg cgctgcgatg cagccccagg gccacccgca
     2701 ggtacttgtc gacctcctgg actacggtat gaacccccag gaggcggtcg agcaccctcg
     2761 ccaccgccac gagaacggcg tcctcctcat cgagggtcgc gtccccgagg aagagatagc
     2821 ccgcctgcga ggtcgaggtc atcgcatcga ggttagggaa gactttatga tccccgcagg
     2881 aggcgcacag ctaatccgca tcctggataa cggtgtcagg gcctgcggca gcgacccgcg
     2941 caaagacggc tgcgcgctcg cacagtaggg ggagtgatga cgccgggaca gggtccagcc
     3001 agaacggttg ctgtcgcggg ggttgtggga gcgctctacg tggtccttag ccttctcgtg
     3061 gcgccgctcg cctacgggcc gattcagttc cggctcggcg aagtattgaa gccgctggtc
     3121 atcacgtatc ctgctaccat ccccgccttc gcggtcgggg tagggatcgt gaacctcttc
     3181 tccccgcaga ccggtggcct ggagttgatt ctgatgccga tagtgaacct ggtgggcggg
     3241 acgctctgct ggttcgtggc acggcggata ggcggcgtgg ccggcaccta catcgcaagc
     3301 ctggtcttcg ccctcgtaat caccgccggt gtcgccacgg tgctccactt taccgtcgga
     3361 ctcccatacc tcctcgcgtt cgggtccgtc gtcgtctccg aagtcgtgtt gctgttgctt
     3421 ggcaacgccg tgctggtacg ccggatctaa ggactcggtg tctccttgcc gctgttcgtc
     3481 tacggcggct aggggctaaa cgcgacgctg gatcatacct cgtcgaccgg ctctccgtca
     3541 tcctgcggta atatagcctc gttgctagaa atcccagacg gcgaggagag cttgtggggc
     3601 agagtagaga gcaggtactc ggccgtgacg tgcggggtcc aatcggggcc gaaggactcg
     3661 atgcggtgcc gtggagccac ggggacaccg acgcggtggt cgagttcacg acgaacgagt
     3721 tgacggcact atgcccgatc acgggacagc ccgacttcta cgagcttgcg ctcacctacc
     3781 gtcctaacgg gtccctgatc gaatccaaat ccttgaaact ctacctgtgg agcttccgcg
     3841 atagcggaat cttcgccgag gacctcgccg ccacactcct gaaagacctc gtctcggcgt
     3901 gcgacccggt agagatgacc gtggacctga cccagcaggt gcgcggagga ctccggattc
     3961 ggaccgtggt ccaccaccct cccgaaatac cagacagcta gccacgagta cctgttcgtc
     4021 gtggcgtcta ccacgaatga gagtattgcc ttgcttcggt gaaccgcctc tggccgaatt
     4081 agccccgcta gcggttcctg ttcttaacaa aacttaatac aactttaaac ctatcttcgg
     4141 actagccgct ggaattaaca tccaggcaag ttagtatgtc gtgcaatgtt tccggaccgc
     4201 ttcgctccgg gtcttcgacc ggagcatggt tcgcatcatc tctactgcgg cgttctgtcg
     4261 caccccgtgt ggttcatatc aacgcgttgt tagtacagga aacgcaagga gaggttgatg
     4321 tttatgcgaa ggtcgtcagg gtcgacggta aacgtggacg catctggtat ctgtgcaggc
     4381 atctcggtag ttttcggatg ccatcctggt gagcgctagg atgaaggacg ccgctgatct
     4441 ctgcctgcac gagctcttcg aggaacaggc gcgccgcacg cccgagaacc ccgccctcga
     4501 agacgccaac atctccctga cctacagcga gctggacagg ctcgctgaca ggctcgcggc
     4561 gtatctcagg accttgggca tcggccagga cgaacccgtc ggtgtttaca tagagcggcg
     4621 ggccgagtac gtggtcgctt gcctcgccgc catgaaagct ggcggcgctt acctgatcct
     4681 cgagctggcc tatccgccgc ccctgctcgc cgacgtcgtg gccgacgcga gcccgcgcat
     4741 cgtcctcacc caggagcggt acgcggatcg tctgccggaa ggtacggaga cattcttcct
     4801 cgatgaaggc tgggaggaga ggctggacga cgtttcggcc gtcgacagta ggcccgagat
     4861 agaccaggac aacctggcct tcgtctcgta ctcgtcaggg acgacgggca agccgaaggg
     4921 gatagccaat ccgcacaggg cgccggtcct ctcttacctg tggcgcttcg gtatcagcga
     4981 ctacgcccct ggcgacaggg tcgggtgcaa cgtgtttttt atctgggaga tgatgcggcc
     5041 gctgttacgc ggggctacca cggtcgtcat ccccgacgac gtcatctacg accccaaagc
     5101 cctgatccgc tttctcgaag agtacgggat cacggagacg ctggttaccc catccctcct
     5161 cgaagccatc ctgaactcga gcgggccaga tgtgggcgaa aagctctcga acctgaggac
     5221 attgtggctg aacggggagg tcgtcacccg cgcgctagca cggcgcgcca tggagctgtt
     5281 gccggacgcg cgcctgctca acgtctacag ttgtagcgag acccacgagg tggcagccgg
     5341 agacctgcgc gaactggtcg agaaccctga gtctacatac tgcgccattg gcgtccccat
     5401 ggacccggag cgcctctatc tactcgacgc tgacggcgaa cgcgtgcccg aggacgaggc
     5461 cggggagctc ttcgtcggtg gtgaatgcct cgctcgcggt tacgtagatc tgcccgagaa
     5521 gaccgaagag agcttcccag aagacccttt ctcctcgagg agcggcgcaa ggatgtaccg
     5581 cacgggcgac agggcccgca tcctctccga tgggagcctc gagatcctcg gccgcgtgga
     5641 ctttatggtc aagatccggg gctacagtgt cgagctcggc gccgtcgagg ccgccatcga
     5701 agagggcctc gccgtaaggt cttgcgtcgt tgtctcggag ggcgaggagg gcgaggacaa
     5761 gcgcctcgtc gcctacatcg tgcctgatcc cggggacgac gagcgctact cgggatggag
     5821 cctcgacccg aagacgggac ggagcaagga gataagggcc atcctgcaag acggcctgcc
     5881 acactacgcg gtcccttccg tcttcgtcga attggagtcg ctcccgatcc aggccacctc
     5941 gggcaaggtc gaccgctccg aactcccgcc tccgccaccc cgccccacca gggaagacgc
     6001 aggtagtttc gccaggctcc ccgaagacgc cccacgctcg gagaaggagg cgctgctagc
     6061 acgggcctgg gaggatgtgc tgcgccttga cgagggcgag gtgcggcccg aggacgactt
     6121 cttcgacctc ggcgggcact cgctcgccgc cgcgcaactc tccagccgcg tggagcaggg
     6181 cttcggcgtc cacgtctcga tgccgctgtt catggaggac ccgaccctcg caggactgtg
     6241 cgacaggata gggacgctac aacgcgacgg gaaagacata cagtcgcagc cctccgaaga
     6301 cttgcgcgcc gaggccgtcc tcgaccccga gatagccccg cagccagccc cgaacggcac
     6361 gcggaccctg cgggacgccg acaacgtctt cctgaccggg gccaccggct tcctcgga
//
LOCUS       DATMJA010000125         2945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_608932, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000125 DATMJA010000000
VERSION     DATMJA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608932"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VNT12_02890"
     CDS             complement(<1..241)
                     /locus_tag="VNT12_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWI45093.1"
                     /translation="MREHHIEDHQVETGVGAGNAAANQLQLDAYGTLLELTWRWHQRG
                     NSPDDDYWRFLMELVENAAKLWNKPDHGIWETRGKP"
     gene            212..766
                     /locus_tag="VNT12_02895"
     CDS             212..766
                     /locus_tag="VNT12_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI45094.1"
                     /translation="MVILDVMLPDSDGVSLCRELRSADSALRDVPVIMVTARDDETSV
                     VVGLEVGADDYVTKPFSPRQLVSRVRAHLRRRRLDSRHPERRLEFPGLTIDLLRREVL
                     AGGRPVELTSREFEVLALLASHPGRVYHREHIMAHLWRGDFYGEARAADVHVQHIHRK
                     IESDPKNPHFIETVRGAGYRFAQL"
     gene            complement(784..1209)
                     /locus_tag="VNT12_02900"
     CDS             complement(784..1209)
                     /locus_tag="VNT12_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWI45095.1"
                     /translation="MPIYLYRLNLGWMLGHRFLLFVHLGRKSGLLRETVLEVILYDPA
                     TPESVVLSAWGEKADWYRNIQAVPALEVRTGGQRYVPEQRFLAPEVNHAVISDYCQRH
                     PLAFRVFARVFGYPLDGTEAARREAACSLRLVAFRPRNR"
     gene            complement(1503..2699)
                     /locus_tag="VNT12_02905"
     CDS             complement(1503..2699)
                     /locus_tag="VNT12_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI45096.1"
                     /translation="MGETGGDMERAPKGHRGGLRLLQLAAFFSTFDRFAIGPMLLTIS
                     ASLGTSLARVAATASLYYLLYGGMQPVWGMLSDRLGRVRVMRLTLFGAVFAGVISALA
                     PNLAILAAARALAGGLFAAVIPAALVYVGDTVGMDSRQKALADLMAASAVGTALATVL
                     GGLAAYLDAWRLAFAAPALAGGVLALLLARLPEPEGFTTEGREGPMVQIGRVFGRFWA
                     VTVVGIAAVEGAVILGFLTYLAPSLESMGFSPATAGLAVGLYGLAVLGWTRAVKLVSN
                     RLGAATLILIGGAMLALGYASGAVEHSLVSVCVAAVLVGGGFAFMHSTLQTWATEVVP
                     EARATVISLFAAALFAGSGLATAAAAPLADDGRFGLLFATAALVAVPLGLFAALARRR
                     YDGHKA"
BASE COUNT          569 a          973 c          932 g          471 t
ORIGIN      
        1 ggggtttgcc acgcgtctcc catattccgt ggtcgggttt attccagagc ttcgcggcat
       61 tctctaccag ctccataaga aagcgccagt agtcatcgtc aggagagttt ccccgctggt
      121 gccagcgcca ggttagctcg agtagcgttc cataagcgtc cagctgcaac tggttggccg
      181 cggcgttgcc cgctccgacg cccgtttcga cctggtgatc ctcgatgtga tgctcccgga
      241 tagcgacggg gtgtccctgt gtcgggagct ccgctcagcg gacagcgccc tgcgcgacgt
      301 gcccgtgatc atggtcaccg cgcgcgacga cgagactagc gtcgtggtgg gcctggaggt
      361 cggggccgac gactacgtga cgaaaccatt ctccccccgt caactcgtca gccgggtgcg
      421 ggcgcatctg agacggcgga ggttggactc ccggcatccg gaacgcaggc tggagttccc
      481 cgggctcacc atagacctcc tacgccgcga ggtgctggcc ggtggtaggc ccgtcgagct
      541 gacctccaga gagttcgagg tcctggcgtt gctggcctcc cacccgggac gggtgtacca
      601 tcgggagcac atcatggcgc acctctggcg cggcgacttc tacggcgagg cccgggctgc
      661 ggatgtacac gtccagcaca tccacaggaa gatagagtcc gaccccaaga acccgcattt
      721 catcgagacc gtgcgcgggg caggttacag gtttgcgcag ttgtgaccct gcagcgaaca
      781 ggactatcgg tttctggggc ggaaggccac caggcgcaag gaacaggcgg cctctcggcg
      841 cgcggcttct gttccgtcca atgggtagcc gaacactcta gcgaaaactc ggaaggccaa
      901 cgggtggcgc tgacagtagt cggagatgac cgcatggttc acttccgggg ccaagaaccg
      961 ctgctccggc acgtaccgtt ggcctccagt tctaacctcc agcgccggaa cagcctgtat
     1021 gttgcggtac cagtcggcct tctcccccca ggcggagagt accacgctct ccggggtagc
     1081 cgggtcataa aggatcacct ccagcaccgt ctcgcggagc aggccgctct tacgccccag
     1141 gtggacgaac aagaggaagc ggtggccgag catccaaccc aggttgaggc ggtagaggta
     1201 gatcggcaac cgaaacgcga gcctcaaagc accggctggc ttcgactcca gccaacggaa
     1261 gtttgggcct agcattccgc aggagggact ttcatccctt ggctagttcg tcgtggctca
     1321 ccataacctt ccacgtctct ctaggtacga gcgtagcatg ccgctgccga tggcctgcaa
     1381 aggaatcgta aaggtccagt taaggatctg tggtaggcac cggcaggcca ggatgtagga
     1441 gcgctggcgg tgccgcacta cgcaagcgtc tctttcagcc gccttccgct gttcgtatgt
     1501 ccttacgctt tatgtccgtc gtagcgtcga cgtgccaagg ctgcgaaaag ccccaagggc
     1561 acggccacca gggccgccgt cgcaaagagg agcccgaaac gaccatcgtc agccagaggg
     1621 gccgctgctg cggtggccag accgctgcca gcgaacaggg ccgcggcgaa tagtgagatc
     1681 accgtcgccc gagcctccgg cacgacctcg gtggcccatg tctgcagcgt cgaatgcata
     1741 aaggcgaacc ccccgcccac cagcaccgcc gcgacgcaca cactcacaag cgagtgctcc
     1801 acggcaccgg aggcgtaacc gagggcgagc atggcgcccc cgattaggat gagcgtggcg
     1861 gctccgagac ggtttgagac aagcttgacg gctcgggtcc accccagcac cgccagcccg
     1921 tagagtccga cggcgagccc ggccgtggcc gggctgaaac ccatgctctc gagcgaggga
     1981 gcgagatagg tcaggaagcc caagatcacc gcgccttcta cggccgcgat cccgactacc
     2041 gtgacggccc agaacctacc gaagacccga ccgatctgga ccatcggtcc ttccctaccc
     2101 tccgtcgtga agccttccgg ctcaggcagg cgagcaagca ggagggcgag cacgccgcca
     2161 gccagggcag gggccgcgaa agcgaggcgc caggcgtcca ggtaggcggc tagtcccccg
     2221 agcacggtcg ccaatgccgt gccgactgcg gaggcggcca tcaggtcagc gagggccttc
     2281 tgccgggaat ccatacctac cgtatctccg acgtagacca gggcggctgg tatcaccgcg
     2341 gcgaacagcc ctcctgccaa cgcccgcgct gcagctagga tagctaggtt cggggccagc
     2401 gccgagatca ctcctgcgaa cacagccccg aagagcgtga ggcgcatgac cctgacgcgc
     2461 ccgagcctgt cggagagcat cccccacaca ggctgcatcc ccccgtagag caggtagtag
     2521 aggctggccg tcgccgcgac cctcgcgagc gacgtcccga gcgaggcgga gatggtgagg
     2581 agcatcgggc ctatcgcgaa gcggtcgaag gtactgaaga aagctgcgag ttgcaggagc
     2641 cgcaacccgc cacgatgccc cttgggcgcc cgctccatat cgcctccggt ttcgcccacg
     2701 cgacgactcc tccgtgcgcg tctcgctcaa taccggatcc cgccccatcc catggcgcag
     2761 ttcctccatg cgctcggtaa tcggaagcag ccgtgtgcta cgcgcccgca ccaggcagtc
     2821 ccttggcgga atgagcatcg agtgtaccag tagacaggcc ggcgaccttg ccaacagtaa
     2881 gacccgaaaa cggccacaag cggtcggagt ggtgtcaatg agaattggtt acaccacagg
     2941 gattt
//
LOCUS       DATMJA010000126         4747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_608951, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000126 DATMJA010000000
VERSION     DATMJA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608951"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VNT12_02910"
     CDS             <1..781
                     /locus_tag="VNT12_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HWI45097.1"
                     /translation="VLAYLVKIERHEDIRQVWYGVGAALLVSLAVGGTLFATAGGLEG
                     RAEQIFEGTAMWVAVGFLTYMILWMRRESRNVARSIRQGIDSAVERGGSLALVSLVFV
                     MVLREGVETALFVFGITRTSEPLQVAFGAALGIVGAVALGYAVYAGGKRINLGTFFKV
                     TGAFLILVAAGLLAHGVAEFEEAGLLPAIMPLWDVSSSPVLGAGTLVSEFLTAFFGWD
                     PEANLLELIAWFTYVVVVGYAFLRPQPLPESAAASPATRNN"
     gene            1008..1769
                     /locus_tag="VNT12_02915"
     CDS             1008..1769
                     /locus_tag="VNT12_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWI45098.1"
                     /translation="MNVFGIDVGGSGIKGAPVDTQSGELLEERVRIKTPKPATPEAIV
                     QTAVGVIREAGWDGPVGCGFPAVVKGGVVQTAANIDKAAIGFDLREALQREIDASVSV
                     VNDADAAGLAEIRWGAGRDEDGVVLMLTLGTGIGTSLFVGGRLVPNIEFGHIEVRGKD
                     AEHRAADSARKRDDLSWKGYAERLDEYLHRVEFLLWPDLIVIGGGISKKSEKFFPHLT
                     ARTKVVAAEMHNNAGIAGAALAGIPERAAAKTAEK"
     gene            1884..2285
                     /locus_tag="VNT12_02920"
     CDS             1884..2285
                     /locus_tag="VNT12_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWI45099.1"
                     /translation="MPAVIEQLCEAINQHDPDAMVECFDPDYRSEQPAHPNRGFGGKE
                     QVHKNWSGMFESFPDLGVEVLRQSTEGDVVWSEWHWSATHLQMAGVIVLGIREDRIVW
                     ARLYMEPVEQAGQDIDEAMRTIRGNDQENDE"
     gene            complement(2371..2958)
                     /gene="folE"
                     /locus_tag="VNT12_02925"
     CDS             complement(2371..2958)
                     /gene="folE"
                     /locus_tag="VNT12_02925"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWI45100.1"
                     /translation="MCEERSIDGPRIERAVREVLLAIGEDPGREGLIGTPERVAEAYA
                     HLFSGLDKDPASYLEVSFAADSHDAVLLRDLPLASMCEHHLLPFVGRAHVAYVPEERV
                     VGLSEIVHLVDGFARRPQLQERLTAQVADALYETLGSRGSVVVIEAEQLCMTMRGAQK
                     PGTVTVTSAVRGVFDEEPGRRAEIMALISRGGTRS"
     gene            complement(3165..3386)
                     /locus_tag="VNT12_02930"
     CDS             complement(3165..3386)
                     /locus_tag="VNT12_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45101.1"
                     /translation="MLRVGNVRDEAAMESVRDALDWLGVDYEHVRSEPDEDLFPQTAY
                     FYVPDDSAEDVESTLADLSGEHGFDAEVL"
     gene            complement(3391..3801)
                     /locus_tag="VNT12_02935"
     CDS             complement(3391..3801)
                     /locus_tag="VNT12_02935"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWI45102.1"
                     /translation="MVDERVSKLARLLVDYFIQAGEGDLVLVSGEVGARPLIRALYVR
                     LLQVGATPVTHISLPGMQEIYFEHAQELQLGGTVHLAIGRSYAQTAGKNDASVHTDLV
                     CGLREGGELYADGELSQENGGFLEFDLAGVNDAR"
     gene            complement(3919..>4747)
                     /locus_tag="VNT12_02940"
     CDS             complement(3919..>4747)
                     /locus_tag="VNT12_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWI45103.1"
                     /translation="PLDARPVSGDPARRALMAGPVIFSTGSLYPFGLDRVYGWISEAR
                     YDGVEIMMDERWDTHQEEFLGDLVERHGVPILALHPPLRRGAWGLGPEETLVRVARLA
                     SRMEVPVVVSHPPPPGRLLERWKAGPLSEAREQGVAVAVENMPQSREGGLFRVRRKSC
                     YLPEHLADVGEVTLDTSHVGASKVDLLRAHSALAVQLRHVHLSDSNLEVGRDEHRLPG
                     KGRLPLKPFLAALGASGYTGAVSLELKPWPLGAPDPEAILERMRAALRFTREGLGTI"
BASE COUNT          893 a         1477 c         1500 g          877 t
ORIGIN      
        1 agtgctcgct tatctcgtca agatcgaacg ccacgaggac atcaggcagg tgtggtacgg
       61 ggtcggtgcg gccctactgg tgtcgctggc ggtgggaggg actttattcg cgacggctgg
      121 cgggttggag gggagggcgg agcagatctt cgagggcacg gcgatgtggg tcgccgtggg
      181 gttcttgacg tacatgatcc tctggatgcg gcgagagtcg cgcaacgtgg cgcggagtat
      241 tcgccagggt atcgacagcg ccgtggagcg gggtggcagt ctcgcgctgg tctcgctggt
      301 cttcgtgatg gtcttgcgcg agggggtcga gaccgcgctc ttcgttttcg ggatcacgcg
      361 tacttcggag ccattgcagg tggctttcgg cgcggcgctc ggtatcgtgg gggccgtggc
      421 gttgggttac gccgtctacg ccggagggaa gcgcataaac ctcggcacgt tcttcaaagt
      481 gaccggggcc ttccttatcc tggtcgccgc ggggctcctg gcccacggtg tggccgagtt
      541 cgaggaggcc ggtctactcc ccgccatcat gccgctgtgg gacgtcagca gttcacccgt
      601 gctgggcgcg gggaccctcg taagcgagtt tctcaccgca ttcttcggct gggaccccga
      661 ggcgaacctg ctggagttga tcgcctggtt cacgtacgtc gtggtggtcg gatacgcatt
      721 cctgcggccg cagccgctcc ccgagagtgc cgccgccagc cccgctacgc gtaataacta
      781 gccttcctgg tcgaacctac aagattccta ctgtctacac aggtcccgta cgaggctaac
      841 gcttctctat cgaagcgtta gcctcgtaac agtattctcc gagggacgta cttggccatc
      901 ctacgattct aggggactcg ccgccaattg atgtcggcca tgccggcccc tcgggcggga
      961 gggtgggatc gtatagaatg acggcggtat ccgtttgggg aggaagcatg aacgtgttcg
     1021 ggatagatgt tggagggtcg gggatcaagg gcgcgcccgt ggacacgcag tcgggtgagc
     1081 tgctcgagga gagggtgagg atcaagactc ccaagccggc cacacccgag gcgatcgtcc
     1141 agacggcggt cggggtgatc cgcgaggcag ggtgggacgg ccctgtcggc tgcggcttcc
     1201 ctgccgtcgt caagggcggc gtcgtgcaga cggccgcgaa catagacaag gctgcgatcg
     1261 gcttcgacct gcgtgaggcg cttcagagag agatcgatgc ctccgtgagc gttgtaaacg
     1321 acgctgatgc ggcgggccta gctgagatcc gctggggcgc tggaagagat gaggacggcg
     1381 ttgtcctgat gctcacgctg ggtaccggca taggcacctc ccttttcgtc ggcggtcggc
     1441 tggtgccgaa catcgagttc gggcacatcg aggtcagggg caaggacgcc gaacaccgcg
     1501 ctgcggacag cgcccgcaag cgcgatgatc tgagctggaa agggtacgcc gagcgcctcg
     1561 acgagtacct ccacagggtc gaattcctgc tctggcctga cctcatcgtc atcggcggcg
     1621 gcataagcaa gaagtctgag aagttcttcc cccacctcac ggcgcgcacg aaagtggtgg
     1681 cggccgagat gcacaacaac gccggtatcg ccggggccgc cctggccgga atccccgaaa
     1741 gagcagccgc caagaccgcc gaaaagtaat aatacgagac aacatagagg tctatgcgac
     1801 gggcctgtgg cgaaaagctg tgccgcgtca gcctggatgg catcatgtgg tgggaatgct
     1861 ggggccggga aggaggcggt gtaatgccgg cggtgatcga acagctctgt gaggccataa
     1921 accagcatga ccccgatgcg atggtggagt gcttcgaccc agattacagg agcgagcaac
     1981 cggcccaccc gaaccggggc ttcgggggca aggaacaggt gcacaagaac tggtcgggca
     2041 tgttcgagag cttccccgac ctcggggtcg aggtgctgag gcaaagcacc gaaggagacg
     2101 tcgtgtggag cgagtggcac tggagcgcca cgcacctgca gatggcaggg gtaatcgtac
     2161 tggggataag agaagatcgg atcgtctggg cgcgcctgta catggagccc gtcgaacagg
     2221 cgggccagga catagacgaa gccatgagaa ccataagagg aaacgaccag gagaacgacg
     2281 agtaatccgt cccctgcccc acaccaggtc aggacgccct gacaccgtat ttggttattg
     2341 cgattcgcag cggtacagat ccacaatcct ctaactacga gtcccgccgc gtgagataag
     2401 tgccataatc tcagccctac gtcctggttc ctcatcgaag acgccccgga ctgctgaggt
     2461 caccgtaacg gtgcctggct tctgggcgcc tctcatggtc atgcacagtt gctcggcctc
     2521 gatgacaaca acggaacccc gagatcccaa ggtctcatat agcgcgtcgg cgacctgtgc
     2581 ggtaagacgt tcctgaagtt gcggcctacg ggcgaagcca tcgaccaggt gcactatctc
     2641 cgacagcccg acgacgcgct cctcgggcac gtatgcgacg tgcgccctgc ccacaaaggg
     2701 gagcaggtga tgctcgcaca tgctcgcgag cggcaggtcc cgcaacaaga ccgcgtcgtg
     2761 gctgtctgct gcgaaggaga cctccaggta gctggccggg tctttgtcta ggcccgaaaa
     2821 gaggtgggcg tatgcttcgg cgacgcgttc aggagtcccg atcaagccct cccgtcccgg
     2881 gtcctcgccg attgcgagca gaacctcgcg cacggcccgc tcgatgcgcg gtccatctat
     2941 gcttcgctct tcgcacatcc ttctagcccc agatccatcc acagaagcgt accgtcgacg
     3001 taaatgaaaa cggcgcgtag ctcatcccac cttcttctcg gcccttatac actgttcgga
     3061 ctaccaaaac ggtacacatt gccttctgat ccacatacct gaagcctgga tacctgttgc
     3121 ctgacaccgc gctgtgcgga aggctagcgt tccgcacagc gctttcagag gacctcggcg
     3181 tcgaagccat gctctccgga gagatcggcg agcgtcgact ctacatcctc tgccgagtcg
     3241 tcgggtacgt agaagtaggc ggtctgggga aagaggtcct cgtcgggctc ggagcgtacg
     3301 tgctcgtagt ctacaccgag ccagtcgagt gcgtccctga cgctctccat cgcggcctcg
     3361 tctctgacgt tccccacgcg caacatcgcc tcacctggca tcattcaccc ccgcgaggtc
     3421 gaactccaga aagccaccgt tctcctggct caactcccca tcagcgtaca gttctccgcc
     3481 ttcgcgcagg ccgcagacga gatccgtgtg cacgctggcg tcgttcttgc cggcggtctg
     3541 cgcgtaggag cggcctatag cgaggtgaac agtcccgcca agctgaagct cttgcgcatg
     3601 ctcgaagtag atctcctgca tccctggcaa gctaatatgt gttactggag tggcgcctac
     3661 ctgcaatagc cgtacataga gggcccttat caggggcctg gcgcccactt ccccggagac
     3721 gaggaccagg tccccctccc cggcctgaat aaagtagtcc accagtagcc gtgcaagctt
     3781 gctgaccctc tcgtctacca ttgctagctc ctttgctgaa gaaattcccg gagagcttat
     3841 catccgcgcc tccccgacag ctttgcacac agaggagcgc gacccaaatg gtaaagcctc
     3901 gggtggggcc gtctctgcct agatcgtgcc gagtccctca cgcgtaaacc tcagcgccgc
     3961 gcgcatcctc tccaggatag cctcgggatc tggagccccc agcggccagg gcttgagctc
     4021 caggcttacg gcaccggtat aaccgctcgc ccccagcgcg gccaggaaag gcttcagggg
     4081 gagccggccc ttgccaggca ggcggtgttc gtctcggccc acctccaggt tcgagtcgga
     4141 cagatggacg tgccggagtt ggaccgctag ggccgagtgg gccctgagga gatcgacttt
     4201 gctggccccg acgtgactgg tgtccagtgt tacctcaccc acgtccgcca ggtgctcggg
     4261 caggtaacag ctcttcctcc taaccctgaa aaggcctcct tcccggctct ggggcatgtt
     4321 ctccacggcc acagctacgc cctgctcccg cgcctcgctc aggggccccg ccttccacct
     4381 ctccagaagc ctgcctggcg gtggcggatg cgacactaca acaggcacct ccatcctact
     4441 cgccagtctc gcgacccgca ctagcgtttc ttcaggacca agcccccacg cgccgcgtcg
     4501 aagaggaggg tggagggcca ggatgggcac gccgtgcctc tcgacaagat ctcctagaaa
     4561 ttcctcctgg tgggtatccc agcgttcatc catcatgatc tccaccccgt cgtaccgggc
     4621 ttccgagatc caaccgtata ccctgtcaag gccgaaaggg tagagcgagc ctgtactgaa
     4681 gatgacgggg cccgccatca gcgctctccg cgccggatct ccagaaaccg gtcgagcgtc
     4741 gaggggg
//
LOCUS       DATMJA010000127         5050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_608998, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000127 DATMJA010000000
VERSION     DATMJA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608998"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1236)
                     /locus_tag="VNT12_02945"
     CDS             complement(97..1236)
                     /locus_tag="VNT12_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWI45104.1"
                     /translation="MDRPVTSGRTAYTVIGLVFALLLGGYFAYRTSGVILAFLLTILL
                     SIILSAPVNYLARRGWPRTWGVIAVIAAIGGVFWLLGLALVPAVETQSREFAQAFPTL
                     LDEALALANRLQSFFGLGTQIGLNPESFSQLGQEVLTGSTVSTAAGVGLTAATVVSLG
                     VVVFISTIYMVIRPEPWVNGFVSLFPAGWRQRTREVLQILYQTVQRWFLGQLAAMTFI
                     AVFWAISLALIGVPFALLLGIFSGLISFIPYLGALISVVLPVLLALISDPFSAVWVIL
                     AFIIIQQIEGNLLQPIVMSRAVDLHPALVIFAILVMGTLFGLIGVFLAVPLVAVLQVL
                     VRELWVKKMDQMGTDPNPPAREPPKRKLPRPIDKALRALRPSRRA"
     gene            complement(1305..1580)
                     /locus_tag="VNT12_02950"
     CDS             complement(1305..1580)
                     /locus_tag="VNT12_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45105.1"
                     /translation="MLLLDEPLAAMGAKEGALILDLIQRLKDERCIPMILIVHNYAQV
                     FDVCDRVDLLTNGRIEYDKPVAESSVEELTELVVSEYRKAGVTGNGG"
     gene            complement(1567..2352)
                     /locus_tag="VNT12_02955"
     CDS             complement(1567..2352)
                     /locus_tag="VNT12_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI45106.1"
                     /translation="MIDLAGRKALVTGAGMGIGQGIAVELACRGAEVAVHFAHTEPEE
                     TVSQIEKLGARAATVQGDLSKVADCERTVDTAAEALGGLDTLVNNAGVTRMLDFLDTD
                     EETYDSVFDLNMKGYFFLARRALPLMMERGGGSIVNITSVHGFAGFPNHTAYAATKGA
                     IVAFTRQLAIELTDRKIRVNAVGPGIIEVPRYFDIPNYTTEFGDTLVPWGRVGYPEDV
                     APAVAFLVSDAADFVTGQVLYVDGGTTARMGLWWELGREDAAP"
     gene            complement(2349..3515)
                     /locus_tag="VNT12_02960"
     CDS             complement(2349..3515)
                     /locus_tag="VNT12_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWI45107.1"
                     /translation="MKITHVDTFVVGTEWRNLTIVRAHTDEGLTGLGEARMVNHTDAL
                     VGYLAEVAPRHLVGHDPFRIEELVKKMGRDDFSRAGEVMSSGIAMFETACWDIVGKAL
                     DQPVYNLLGGPVRDRVKAYANGWYRVERTPEEFHEAAKKVVERGYRALKLDPFGPGHY
                     ELEYEDKIQAVSLVEAVRDAIGPEVEILLEMHGRFSPATAIEMARMLQPFGLGWVEEP
                     VPPVNLKALKKASDGIEATVATGERIHQRLEYRELFELQAADVIQPDISHIGGLLETK
                     KLAAWADAYYVTIAPHNVGGQINTAAALHLAACTTNFRIQEYFNDFADPWVRETAPGL
                     SEVTDGYFELPKGPGLGVELDEEVIEAHPKQHIHFNLFAEGWERREGARTGADQ"
     gene            complement(3512..4471)
                     /locus_tag="VNT12_02965"
     CDS             complement(3512..4471)
                     /locus_tag="VNT12_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HWI45108.1"
                     /translation="MKAHELLSGLGLPGRDLYDLPDSEKRFPDGAQYRVEIPSVEGPR
                     VLEAVIEEAERREVQIHRVSQGSGIMLLTDAEILEMCHMAREAALELSLFVGPRASWE
                     TGATAVSGAGRVLGAQHRGQDQLAYALEDVLRGVSLGLRGVLVADAGLLWVLRDLKTK
                     GELPEDLVLKVSVQLAAANAAAVKAMEELGADTYNVPTDLSLAQLAAIRAAADLPLDV
                     YVEVPDDFGGFVRHYEIPDLVRVASPVFVKFGLRNAPNIYPSGTHLEATAVALGRERV
                     RRARIGQEMLARYYPEAETTPPGITFPGLPVEAEANAEVKEKA"
     gene            complement(4468..>5050)
                     /locus_tag="VNT12_02970"
     CDS             complement(4468..>5050)
                     /locus_tag="VNT12_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWI45109.1"
                     /translation="ALNLLEARGLISIEHGRGAVVSGGNPQAVRDTLGLLLRVQPKTL
                     WELLEIRKILEVEVAGLAAERAGPDDVATMRLQLDRMRESIDAPEGYVDADVEFHALL
                     ARGTRNEVLLTMLEPVVDLLRASRRVSAARPGNALRALGEHERILRRVEARDAGGARE
                     EMRVHLAKTAEDIEAAIGEGVLDAGELLEKEGV"
BASE COUNT          857 a         1614 c         1627 g          952 t
ORIGIN      
        1 gcctgcacaa gtcctggctg actggctgac aagtcgcctg gaacaggcga cgtgctgaca
       61 agctgacttg cccgtcgtgc ataaggcttg agagccctat gcacggcgtg agggacgtag
      121 tgctctgagg gctttgtcga tcgggcgtgg gagtttgcgt ttggggggtt ccctggcggg
      181 tgggttgggg tcggtcccca tctggtccat ctttttcacc cagagctcgc gcaccagaac
      241 ctgcagcacg gcgacgagtg ggacggccag gaagactcct ataaggccga agagcgtgcc
      301 cattaccagt atggcgaaaa tgaccagtgc gggatgcagg tccacggccc ggctcattac
      361 gatcggctgg agtaggttgc cttcgatctg ctggatgatg ataaaagcca ggatcaccca
      421 gacggccgag aaagggtctg agatcagagc cagaagcact ggcaggacga ccgagatgag
      481 cgcgcccagg taggggatga acgagatcag gccgctgaaa ataccgagga gtagcgcgaa
      541 aggcaccccg atcagggcga gggagatggc ccagaaaaca gcgatgaagg tcatggccgc
      601 gagttgaccg aggaaccacc gctgtactgt ttggtacagt atctggagca cctctcgcgt
      661 cctctgccgc catcccgcgg gaaagagcga gacgaacccg ttgacccagg gctcgggccg
      721 gatgaccatg tagatggtgg agataaacac gaccaccccg agcgagacca cggtcgccgc
      781 ggtcagcccc acgccggcgg ccgtcgatac ggtgcttccg gtgaggacct cctgcccgag
      841 ctgtgagaaa ctttcggggt tcaggcctat ctgggtacca agcccgaaga agctctggag
      901 acggttggcc agggcgaggg cttcgtccag gagcgtcgga aaggcctggg cgaactcgcg
      961 agattgggtc tcgacggcag ggacgagtgc cagaccgagg agccagaaca cacccccgat
     1021 cgccgcgatc accgcgatta ccccccacgt gcgtggccat ccccggcggg cgaggtagtt
     1081 gaccggagcg ctgagtatga tagacaggag gatcgtcagc aggaacgcga ggatcacacc
     1141 gctggtccgg taggcgaaat acccgcccag gagcagcgca aagaccagcc cgatgaccgt
     1201 gtacgccgtt cttcccgagg ttacgggtct atccactccg actccttcgg ctgtccctat
     1261 ggccggagtt tacttgctga tagtgggttg gggaagggtc gaccctaccc gccgttgccg
     1321 gtcactccgg ctttgcggta ctcggagacg acgagttcgg tgagttcttc gacggagctc
     1381 tcggcgaccg gcttgtcgta ttctatgcgg ccgtttgtga ggaggtcgac ccggtcgcag
     1441 acgtcgaaga cctgggcgta gttgtggacg atcaggatca tgggtatgca ccgttcgtct
     1501 ttgaggcgct ggatgaggtc gaggatcagg gctccctcct tggcacccat cgccgcgagc
     1561 ggctcgtcaa ggagcagcat cttcgcgtcc gagttcccac cagaggccca tcctggccgt
     1621 ggtgccgccg tccacgtaga ggacctgccc tgtgacgaag tcagcagcgt cggagacgag
     1681 gaaggctact gcgggggcga cgtcttcagg gtatccgaca cggccccagg ggaccagggt
     1741 atcgccgaac tcggtagtgt agtttgggat gtcgaagtag cgggggacct ctatgatgcc
     1801 agggccgacg gcgttgacgc ggatcttgcg gtctgtgagc tctatggcga gctggcgggt
     1861 gaaggcgacg atcgcgccct tggtagccgc gtaggccgtg tgattgggga agcccgcgaa
     1921 cccgtggacc gaagttatat tgactatgct cccgcctccc ctctccatca taaggggcaa
     1981 ggccctgcgg gccaggaaga agtagccctt catgttcagg tcgaagaccg agtcgtaagt
     2041 ctcctcgtcg gtatcgagga agtctagcat cctggtgacg ccggcattgt tgacgagcgt
     2101 gtcgagtccg ccgagggcct cggctgcggt gtccacggtc ctctcgcagt cggcgacctt
     2161 gctcaggtcg ccctggaccg ttgcggccct ggcgccgagc ttctctatct gcgagacggt
     2221 ctcctccggc tccgtgtggg cgaagtggac ggcgacctcg gccccgcggc aggcgagctc
     2281 gacggcgatg ccctggccta tccccatccc cgcgcctgtg accagcgcct tcctacctgc
     2341 gaggtcgatc actggtcagc ccccgtgcgg gcgccctcgc gcctctccca gccctcggcg
     2401 aagagattga agtggatgtg ctgcttgggg tgggcttcga tgacctcttc gtccaactcg
     2461 acgccgaggc ctggtccctt tgggagctcg aaatagccgt ctgttacttc ggagaggccc
     2521 ggggcggtct cgcggaccca ggggtcggcg aagtcgttga agtactcctg gatgcggaag
     2581 ttcgtggtgc aggcggccag gtggagtgcg gcggccgtgt tgatctggcc tccgacgttg
     2641 tgcggggcga tggtcacgta gtaggcgtcg gcccacgctg cgagcttctt cgtctccagg
     2701 agcccgccga tgtggcttat gtctggttgg atcacatctg ccgcctgaag ctcgaagagc
     2761 tccctgtact ccagccgctg atgtatgcgc tcgccagtgg caacggtcgc ctctataccg
     2821 tcagaagcct ttttcagcgc cttcaggttc acgggaggca ccggctcctc gacccagccc
     2881 agaccgaagg gttggagcat cctggccatc tctatggcgg tagcgggaga gaagcggccg
     2941 tgcatctcca gcaggatctc gacttcgggt cctatggcat cgcgaacggc ctcgacgagc
     3001 gagacggcct gtatcttatc ctcgtattcc agctcgtagt gtcctggacc gaaaggatcc
     3061 agcttcagcg ccctgtaccc acgttctacg accttcttcg cagcctcgtg gaactcctca
     3121 ggcgtgcgct cgaccctata ccagccgtta gcgtaggcct tgaccctatc cctcacaggc
     3181 ccgccgagca ggttgtagac gggctggtcg agcgccttgc cgacgatatc ccagcaagcc
     3241 gtctcgaaca tcgcgatgcc gctgctcatc acctcgccgg cgcgcgagaa gtcgtcgcga
     3301 cccatcttct tgacaagctc ctctatcctg aacgggtcgt gcccgacgag atgcctcggg
     3361 gcaacctcgg ccaggtaccc gaccagggcg tccgtgtggt tgaccatccg cgcctcgccg
     3421 aggcctgtca atccctcgtc tgtgtgggcg cggacgatgg tcaggttgcg ccactcggtg
     3481 ccgacgacga acgtgtctac gtgggttatc ttcatgcctt ctccttcacc tcggcgttcg
     3541 cctcggcttc gaccggcaag ccggggaagg ttatgcccgg cggcgtagtc tcggcctcgg
     3601 gatagtagcg cgcgagcatc tcctgtccga tcctggctcg cctgactctc tccctcccga
     3661 gcgcgaccgc cgtcgcctcc aggtgcgttc cgctggggta gatgttcggt gcgttcctca
     3721 ggccgaactt gacgaagacg ggcgaggcga cccttacgag gtctggtatc tcgtagtgcc
     3781 tcacgaaccc gccgaagtcg tcgggcacct cgacgtacac gtcgagcggg aggtcggcag
     3841 cggcccgtat cgcagcgagt tgggccagcg acaggtctgt gggcacgttg tacgtgtccg
     3901 cccctagctc ttccatcgcc tttacggccg ccgcgttggc agcagcgagc tgcacggata
     3961 ccttgagaac gaggtcctcg ggcaactctc ctttagtctt gaggtcccta aggacccaga
     4021 ggagtccggc gtcggcgacc aggaccccgc gcagtcctaa agagacgccg cgtagcacgt
     4081 cttctagggc gtaggcgagc tggtcctggc cgcggtgctg ggccccgaga accctgcctg
     4141 cgccggagac ggcggtcgcg ccggtctccc acgaggcccg cggtccgacg aagagcgaga
     4201 gctccagggc ggcctcgcgc gccatgtggc acatctccag gatctcggca tccgtgagga
     4261 gcatgatccc tgacccctga gagacgcggt ggatctggac ttcccttctc tcggcctcct
     4321 cgataaccgc ctcgagcacc cgcggtcctt cgacgctcgg gatctcgacc ctgtactgcg
     4381 ccccgtcagg gaaacgcttc tccgagtctg gcaagtcgta gaggtcgcgt ccaggcaacc
     4441 ccagcccgga caatagctcg tgggccttca taccccctcc ttctctaaga gctctccggc
     4501 gtcgagtacc ccttcaccga tggcagcctc gatgtcctca gccgtcttcg cgaggtggac
     4561 gcgcatctcc tcccgcgcgc ccccggcatc tcttgcctcc acccgtcgca gtatccgctc
     4621 gtgctcccct aacgcccgta gcgcgttacc aggcctggcg gcgctcaccc ggcgactcgc
     4681 ccgcagtagg tcgaccacgg gctcgagcat ggtcagcaag acctcattgc gggtcccccg
     4741 cgccagcagc gcgtggaact cgacatcggc gtcgacgtag ccctcgggtg catcgatgga
     4801 ttcccgcatc ctgtcgagct ggagccgcat tgtcgccacg tcatcgggcc cggcacgctc
     4861 cgccgcgagg cctgcgacct cgacctccag tatcttgcgt atctccagaa gctcccatag
     4921 cgttttcggt tgtacgcgca ggagcagacc gagcgtgtcg cgcacggcct gcggattgcc
     4981 accgcttacc acggcgccgc ggccgtgctc gatgctgatg aggcctcggg cctcgagcag
     5041 gttcagagct
//
LOCUS       DATMJA010000128         2872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_194755, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000128 DATMJA010000000
VERSION     DATMJA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194755"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VNT12_02975"
     CDS             <1..613
                     /locus_tag="VNT12_02975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWI45110.1"
                     /translation="AHAGAGMDLAEARKPMVFDLGGTKIGVLSYLRVPSYEWAWATQT
                     APGTAPLVQTVMEEDIKRLRPNVDLILVMPHWGKEYIATPEPKQVDLAHAAIDAGADL
                     FIGDHAHWPKGIEMYKGKPIFYGVGNFLFDQSWSEETSTAIFAETTLYGDDVVQVRPV
                     PFVILDYAQPNFLTPGGGGDRALRKIFAASLGPEFEAYGRRPR"
     gene            1062..1544
                     /locus_tag="VNT12_02980"
     CDS             1062..1544
                     /locus_tag="VNT12_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWI45111.1"
                     /translation="MDHAPPDNGGGHAFLRCSILRRTGGRRNGAGLFRKEKGEGGRRL
                     ASLATQPLVVDFLDVVTRGEKGIEFRLEEFFVPEPSTVAEHTIGELKIGERTGAIVLA
                     IRTTEGNFDTTPSADDRLRAGDTLIVLGSRGQIERLEQLMRGMCRGASRSTPTHTVSP
                     "
     gene            complement(1559..>2872)
                     /locus_tag="VNT12_02985"
     CDS             complement(1559..>2872)
                     /locus_tag="VNT12_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45112.1"
                     /translation="GPLLDQSVRLAQFQNIYRTDLTVSPYVVSNYPPLFMLAQVPLVW
                     VFGPALWYGRLISLLSVAAVALFVGLTLHALTKDRIAAIAGGLIFPAMPYVVRWSSLS
                     RVDLLGLALSWAGLFVVARWPEKRWSVFVSALLLIAAVYTRQTYVLAAPLAAFVWLAA
                     QGQRRRALETAAIAGGAGLIVFALLNIFTGGGFFLNTVTANLNDFRWERVSLNALGAL
                     LACPLLLLGSLAFLLFTPRKGNRSWWLVAPYLLASIPSAVLVGKVGSDVNYLLELSAA
                     LGLATGAFIAWQRRRPRLRIALIALLAVQVLALAQSSRVPSGLQTYVIEQRGEVAQLS
                     RMVANANGPVLTDDYMGLLPQSGRRIYFQPFEMTQLTLDGDWDQRPILKDIDQERFPV
                     IMIWSPPFAREIKRDRWTPRMLEEIHDNYERTDRVADMVIYRPRE"
BASE COUNT          589 a          879 c          901 g          503 t
ORIGIN      
        1 agcccacgct ggggcaggta tggatcttgc ggaggcccgc aagcccatgg tgttcgacct
       61 cggtggaacg aagatcggtg tcctctctta cttgagggtg ccttcctacg agtgggcctg
      121 ggctacccag acggcacccg gcacggcgcc gctcgttcag accgtcatgg aagaggacat
      181 aaagcgcttg cgccctaacg tcgacctgat cctcgtgatg ccgcattggg gcaaggagta
      241 catcgcgacc ccggagccta aacaggtgga tctcgcgcac gccgcgatag atgccggggc
      301 cgacctattc atcggtgacc acgcccactg gcccaagggg atcgaaatgt acaagggcaa
      361 gccgatcttc tacggcgtcg gtaacttcct cttcgaccag tcctggtcgg aagagacctc
      421 gacggcgatc ttcgcggaga ccacattgta cggggacgac gtggtccagg tccgccccgt
      481 cccattcgtc atcctcgact acgcccagcc caacttcctc acgccagggg gaggcgggga
      541 tagggcgttg cgcaagatct tcgccgcatc cctcggtccc gagttcgagg cgtacgggcg
      601 ccgcccccgc tgacgttcct tcgatgaacc cggacgcgct gaacaaccaa ctggtcggaa
      661 tcgtcaggca gacgatacga ccggcgcacg tttcgctgtg gctgcgtcac gatacaccca
      721 tgaaggcaaa gtaggaagac cagccgactc ttgctttgtg agccggacgg cgtcctaccg
      781 agggaggcaa gccgctcgac tataattccg cctgtggcgg aggacgagcg caggagatcc
      841 agcagcagtg acttcctcga gaggaggtct tacgaaccac ggcttgcgct gcggcgggtg
      901 caaagcgccg ttctggcgct cgtaatcatt accgctgtag gcatttttgg gtacgtggtt
      961 ttcgagggat ggaacttcac cgatgcgctt tacatgaccg tgatcacgct taccacggtg
     1021 ggatacagag aggttcgcgt gcttgatgta tcagggcagc tgtggaccat gctcctcctg
     1081 ataacggggg tgggcacgct tttctacgct gcagtatcct ccgtcgaact ggtggtcgaa
     1141 ggaacggtgc ggggctattt cggaaggaga agggtgaagg cggcaggcgg ctcgcctcac
     1201 tcgccacgca gcccctcgtc gtcgactttc tggacgtcgt cacgcgcggc gagaaaggca
     1261 tcgagttccg cctggaggag ttcttcgtgc ccgagccttc gaccgtcgcc gagcacacca
     1321 tcggggagct caagataggg gagaggaccg gcgcaatagt cctcgctatc cgcaccacag
     1381 aaggcaactt cgacaccacg ccatcggccg atgatcgtct gcgcgccggc gacacgctca
     1441 tcgtcctggg gagccgcggc cagatagaac gcctggagca gctcatgcgc ggtatgtgta
     1501 ggggcgcttc gcgaagcacc cctacacata ccgtctcgcc atagacacga accgccccct
     1561 attccctcgg acggtagatc accatatcgg cgacacgatc cgttctctca tagttgtcgt
     1621 gtatctcctc gagcatcctc ggagtccacc tgtcacgctt tatctctctt gcgaagggtg
     1681 ggctccagat catgatcacg ggaaacctct cctggtcgat atctttcagg attggtctct
     1741 gatcccaatc gccgtcaagg gtgagctggg tcatctcgaa aggctggaag tagatcctcc
     1801 gtccactctg gggtaggagt cccatgtagt cgtctgtgag cacgggcccg ttcgcattcg
     1861 ccaccatacg tgatagttgg gcgacctcgc cccgctgctc gataacgtag gtctgcaaac
     1921 ccgaagggac ccgagacgat tgtgcgagcg ccagtacctg gacagcgaga agggctataa
     1981 gggcgatccg caaccgcggc cgcctgcgct gccaggcgat aaatgcccct gttgcaaggc
     2041 cgagcgccgc cgacaactcc aacagatagt tcacgtcaga acccaccttg ccgacaagca
     2101 ccgcggaggg gatgctcgcg agcagatagg gtgcgacgag ccaccatgaa cggttcccct
     2161 tgcgcggcgt aaacagtaag aacgcgagcg agccgagcag tagcagcggg caggcaagga
     2221 gggctcctag ggcgttgagg ctaacccgct cccaacggaa atcgtttagg ttggcggtta
     2281 cggtgttgag gaagaacccg cctccggtga agatgttcag tagcgcgaac acgatcaggc
     2341 ctgctccgcc ggctatagct gccgtctcca gcgcccggcg gcgctggccc tgggctgcga
     2401 gccacacgaa ggccgcgagc ggcgcggcca gtacgtaggt ctggcgggta tagacggcag
     2461 ctatgagaag cagggccgag acgaagacgc tccagcgctt ctcgggccag cgcgcgacga
     2521 cgaacagccc ggcccaggag agcgccagcc ccagcaagtc gaccctggag agcgatgacc
     2581 agcgcaccac gtagggcatg gcagggaaga tcagaccccc tgctatcgcc gcgatcctgt
     2641 ctttggtaag cgcatgcagg gtcagcccca caaagagcgc cacagccgcg acgctgagca
     2701 gcgagatcag acgcccgtac cagagcgcag gcccgaacac ccacactaga ggcacctggg
     2761 cgagcatgaa cagcggagga taattggaga cgacgtaggg tgagacggtt aggtcggtcc
     2821 tgtagatgtt ctggaactgc gcgagcctta cggactgatc gagcagcggc cc
//
LOCUS       DATMJA010000129         3243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_787462, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000129 DATMJA010000000
VERSION     DATMJA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787462"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VNT12_02990"
     CDS             <1..486
                     /locus_tag="VNT12_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45113.1"
                     /translation="PGLGGFIDFLFDRDPTGGFLAGILIGAAFPGLVFLFFYLWRYFR
                     AWHRLRRAPLEGPTVWRREVVVVVDPACRNDSLLEVCKKALQVMGGHVTEIDLKQNRL
                     AGKLGRRASGYDEISVHIKRGANGDCSLTIASDGVRPTVTDDKSRHFSNLRRFTELII
                     G"
     gene            complement(716..1237)
                     /locus_tag="VNT12_02995"
     CDS             complement(716..1237)
                     /locus_tag="VNT12_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWI45114.1"
                     /translation="MRLEGKRIAVLVAEGFEDLEYWVTVMRLQEEGAEVVSVGPDTEP
                     VSGKNALKARADTTADGVDATGLDGVVVPGGWAPDKLRRYPEITNLVSTVHDRNKTVG
                     IICHGGLVAISAGIVDGARATGSLGIKDDLENAGATWVDKAAFREGNLVWGRVVPDIP
                     DFCRELVAALAEE"
     gene            complement(1260..>3243)
                     /locus_tag="VNT12_03000"
     CDS             complement(1260..>3243)
                     /locus_tag="VNT12_03000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HWI45115.1"
                     /translation="VSPLISLMKDQVDSLLQSSVSDAATLLSGLSPEERWEVERRVRT
                     GEVKMLYVAPERLRSLEFVLALRRAGVGLFVVDEAHCISEWGHNFRPDYLFLPRAVRD
                     LGNPPVLALTATATPRVRQDILLSLKMRSPEVVVTSFNRPNLTYRVIPAKEKKHKLPR
                     ILDVIRASPPPGIVYATTRKECEELAAGLRRSGVDAAAYHAGMGSADRANIQERFMTD
                     ELAVVVATVAFGMGVDKPNVRFVIHSSVPGSLPAYIQESGRAGRDGESSECVVLYRGA
                     DLGRRKRLVTLGTAGEREVSSFFSALSGVESGGRVNVPPASLSALGGVDPESAGIVMG
                     SLEESGLISRGYDLWAEVEVRAVEEEPKGLREEVAAVHAALPGSGSVGLPELARKAGM
                     RPAVVQGAIYRMMVDGVLEIIPRGSLVDVRLKTFSLDNDSRRNIARRLKNRANTAYAQ
                     IRDVESFANLRTCRREHLLRHFGDMEEVAPCAGCDVCLGESQEHVSIAGSGAVVPEGK
                     PLLPAFAGLSPVDEDLPAVDAALFERLRSWRGEQARKQRVPAYVVLHNSHLEEISARK
                     PGTIHELGAIKGVGLRRAARYGDEILALIRGEEVPDSSAPAAENGFREHLEAAGRLLR
                     SGRGADAVPALARALELGGEEARREVDKLLGSMTGE"
BASE COUNT          588 a         1068 c          954 g          633 t
ORIGIN      
        1 cctggcttgg gtggcttcat agattttctt tttgaccgtg atccaacagg cggtttctta
       61 gccggtatcc tcatcggtgc ggcatttcct gggctagtat ttttgttctt ttacttgtgg
      121 cgctatttta gagcgtggca ccgtctccgt agagccccat tagaaggacc gactgtttgg
      181 cgtagagaag tcgtggtggt ggttgatccc gcttgtcgta acgactcgct gctcgaggtt
      241 tgcaagaagg cacttcaagt gatggggggt cacgttacag agatcgacct aaaacaaaat
      301 cgcttagcag gtaaactggg ccgccgagca agcggatatg atgagatttc tgtccacatt
      361 aagcggggag cgaacggtga ctgctcacta acaatagcaa gtgatggtgt cagacccact
      421 gttacggatg acaagagtag gcacttctca aacctgagac gctttaccga actcatcata
      481 ggctgattta agagtggtct agaaatcacg ttcgtagatg ttgcgttgct cacatagcgt
      541 gggcagtctt ctataatctc caaacttctc ttgtccaatg aggaagacca gcattatcag
      601 cgtcggtggc gtggaagcta agctacgtcg ccccaatgca cccgcgtccg cctacatttc
      661 tcccccagcg tccccatcac ttcatgggtg tgtccactgg tgtgccgtat cctctctact
      721 cctcggccag ggcggccacg agttcgcggc agaagtcggg gatatcgggg accacccggc
      781 cccagaccag gttaccctcg cggaacgccg ccttgtccac ccaggtcgcg ccggcgttct
      841 ccaggtcgtc tttgatccct agcgagcccg tagccctcgc cccatcgact atgccggcag
      901 agatggcaac gagcccgccg tggcagatga tgccgacggt cttgttcctg tcatgcacgg
      961 tactgacgag gttcgtaatc tcgggatagc gcctgagctt gtccggtgcc cagccgccag
     1021 gcaccacgac gccgtccaac cccgtggcgt caacaccgtc cgctgtcgta tcagcccgcg
     1081 ccttgagggc gttcttgccg ctgaccggtt cggtatcagg tccgacggag acgacctcgg
     1141 ctccctcctc ctgcaggcgc atcaccgtga cccagtactc gaggtcctcg aaaccctcgg
     1201 ccacgagaac cgcgatcctc ttcccctcca acctcataga gactcctctc gtatagactt
     1261 cactcgcccg tcatcgatcc tagaagcttg tcgacctcgc gcctcgcctc ctcgccgccc
     1321 agctcgagcg cacgggccag cgcaggcacc gcatcagcgc cgcgccctga ccggagcagc
     1381 ctgccagcag cctcgagatg ctccctgaag ccgttctccg cggcgggcgc actactatcc
     1441 ggtacctcct ccccgcgtat cagggcgagt atctcgtccc cgtagcgggc cgcgcggcgc
     1501 agacctactc cctttatggc gcccagctcg tggatggtgc cgggtttgcg ggcggagatc
     1561 tcctcgagat gcgagttgtg gagcacgacg taagcgggca cccgctgctt tcttgcctgc
     1621 tcacccctcc agcttctcag ccgctcgaaa agcgccgcgt ccacggctgg aagatcctcg
     1681 tccactggtg acaacccggc gaacgcaggc aggagcggct tcccttcagg aacaacagct
     1741 cctgacccgg cgatgctcac gtgctcttgc gactcgccaa ggcagacgtc acagcctgcg
     1801 cagggtgcga cctcctccat gtccccaaaa tgccttagga ggtgctcgcg gcggcaggtc
     1861 ctcaggttgg cgaacgactc tacgtcccgg atctgggcgt aggctgtgtt cgcccggttc
     1921 ttgagcctgc gtgcgatgtt ccttcggctg tcgttgtcca gcgagaaggt cttgagcctc
     1981 acgtccacca gagagccgcg aggaatgatc tccagcacac cgtcgaccat cattctgtag
     2041 atggcaccct ggactacagc aggacgcatc cccgccttgc gcgccagctc gggcagcccg
     2101 acggagcccg agcctggcag cgcggcgtgc accgcggcca cctcctcgcg tagacccttt
     2161 ggctcctctt cgacggcgcg cacctcgacc tcggcccaga ggtcgtagcc gcgggagata
     2221 agtccggact cttcgaggct gcccataacg atgccggccg actcggggtc gacgccccct
     2281 agcgccgaga gcgaagcggg cggcacgttc accctcccac cgctctccac cccggagagc
     2341 gccgaaaaga aagatgagac ctcgcgctct cctgccgtgc caagtgtaac cagccgcttg
     2401 cgccgtccga ggtcggcgcc gcgatacagt acgacgcatt cggaactctc cccgtccctg
     2461 cccgcgcgcc ccgactcctg gatatatgcg gggagactcc ccggaacgct cgaatggatg
     2521 acgaacctga cgttcggctt gtccaccccc atcccgaatg cgacggtcgc gacgaccacg
     2581 gcgagttcgt cggtcatgaa tcgctcctga atgttggccc tgtcggccga gcccatcccg
     2641 gcgtggtacg cggcagcgtc gaccccggaa cggcgcaagc cggcggcgag ttcctcacac
     2701 tccttgcgcg tcgtggcgta gacgatccct ggcgggggtg aggcgcgtat gacgtcgagt
     2761 atgcgtggca gcttgtgttt cttctccttc gcggggatga ccctgtaggt caggttcggg
     2821 cgattgaagc tagtcacgac cacctcgggt gagcgcatct tcagcgagag caagatatcc
     2881 tgccgcacgc gcggagtcgc cgttgccgta agggcgagga cgggagggtt acccaggtcc
     2941 ctcacggcgc gaggcaagaa caggtaatcc gggcggaaat tgtggcccca ctccgagata
     3001 cagtgggctt cgtccacaac gaagaggccg acgccggcgc gccggagcgc gaggacgaac
     3061 tccagggatc tgagtcgctc gggggccaca tagagcatct tcacctcccc cgttcgcacc
     3121 ctccgctcga cctcccagcg ctcctcgggc gagagtccag agaggagcgt ggctgcatcg
     3181 gagaccgagc tctgaagcag ggagtcgacc tggtctttca taagtgagat caggggcgag
     3241 act
//
LOCUS       DATMJA010000130         4569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_76021, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000130 DATMJA010000000
VERSION     DATMJA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76021"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..360)
                     /locus_tag="VNT12_03005"
     CDS             complement(76..360)
                     /locus_tag="VNT12_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45116.1"
                     /translation="MSEVVAIAQIVTAIATVFYAGVFAIGYYMLIKGNRQTLSEMREQ
                     RISGGRPQVIVQGRYDRLPMVDLVVRNLGGPPPRGVIMAPSATDASPRSA"
     gene            complement(501..1349)
                     /locus_tag="VNT12_03010"
     CDS             complement(501..1349)
                     /locus_tag="VNT12_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI45117.1"
                     /translation="MKVHDRRTESSAKKTVKRLPGPGHVLMGQVAYQLRLLARTPRAA
                     FSAFLLPVLLLVAFHLLRSYSGSSDPALVGGLTAFGVIMTGYATHAGAVVAARERGVL
                     KRLRGSPLPPWAYFAGSILATTALCLLSAAVTLLVARLLGMQVRLAEGPALLAVVLFG
                     AISWAALGTAVAAFIPDAGSAGPLLSATYLPVVFVSGVFVPIAAEPGWLATMARWLPA
                     EPFIHALSTTLQAGGSGVAAPPAGDLLNCTLWAVVGLYVALRHFRWEPRGGEHRGHAT
                     REVGAQ"
     gene            complement(1336..2043)
                     /locus_tag="VNT12_03015"
                     /pseudo
     CDS             complement(1336..2043)
                     /locus_tag="VNT12_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2359..>4569)
                     /locus_tag="VNT12_03020"
     CDS             complement(2359..>4569)
                     /locus_tag="VNT12_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45118.1"
                     /translation="ARAVEELGGSNGAEPKLESLGEWLAAETGGQPFYLVETLKALLE
                     EGELVMQMGAHGEPVVEVGPALGAGSDLRGLLPSNVREVIRARLSHLSPAASELLRAG
                     TVLGRSFSFESLVGVAGLGEVEGLRGLDELVERHLLQEEAGGEAEEPLLYPSSVYSFS
                     HEKIRQVAYTESGHARRQVLHRRALEVLEESGAPPAELAPHALAGGLARPAFAYSVAA
                     GDQAMEVFATHDAIEHYERAQNLLAEALTGGRQLAEPSIPDLEHLYIQLGRAYEMIDE
                     WSRSRAAYEALLALGRELGEARLEVVSLNHLAVLDYHQGDIRLVRTLLEEARTVAEEA
                     GLVEALAETECNLADVMTFWVGESEHSRPLAQQALASARALERPDLVARALVALARVE
                     VFRGRLKESAAYAEEGAALSQQLAERPPSRTILPTATSAITGLAASWKAGHKAIEIQC
                     LTYLAYARIFQGRLQEGLAIGQEAHAISRELPERIEAMSAWALSLGLVEIGEYEEALM
                     LARRGVGLAREAQDTYLLAVNLDRLGRAHEALLNLEEARAAYAEPLGQQYEAWSSARL
                     CVVAALSENWEEAYAHARRAIEADVFANNQPLGIHFHHEVEALLRGGDELLAREEVRR
                     FAERAQTNERDRIAYLCSLAILGEWEGDPERAAVPLRQAEALAEKLGLPGELWRIRAG
                     LGGLYERRRETSEARAAFSRAAQTLRKLAERIGDEELREGFLSVPQARRVLERG"
BASE COUNT          759 a         1629 c         1364 g          817 t
ORIGIN      
        1 attcatcata gtcatctgtc cttccagagc ctagcaatgg ggcgtggtaa gggcgtggta
       61 aggcgtggcg aggtgttacg cagagcgtgg tgaggcgtcc gtggcggagg gcgccattat
      121 tacgcccctg ggcggcggcc caccgaggtt tctcacgacg aggtccacca tcggcaggcg
      181 gtcgtagcgt ccctggacta tgacctgcgg ccgcccgccg gagatccgct gctcgcgcat
      241 ctcgctgagc gtctggcggt taccctttat gagcatgtag tagccgatcg cgaagactcc
      301 tgcgtagaag acggtagcta tggcggtgac gatctgggct atggctacca cttctgacat
      361 gtccccgtct ttcggtcgtc gggctggcgg agatactgct cgtggaccct ccggggacag
      421 caacctgctc aacttcaatg tcagtcggcg aaccgggaca ttcttaccgc tcctcgtgga
      481 cagacgctcg ccagcgatcg tcactgcgcg ccaacctccc tcgtcgcgtg ccccctatgt
      541 tcgccaccac gcggctccca ccggaagtgc cgtagcgcga cgtaaagacc aaccacggcc
      601 caaagcgtgc agttcagcag gtcgcccgcc ggtggcgccg cgacgccgga accgcccgct
      661 tggagggtcg tgctcagggc gtggatgaac ggctcggctg gcagccacct cgccatggtg
      721 gcgagccagc cgggttcggc ggcgatgggg acgaagacgc cggagacgaa caccaccggg
      781 aggtaggtcg ctgacagcag tggcccggcc gagccggcat ccgggatgaa cgccgcgact
      841 gcagtgccga gcgccgccca ggagatcgca ccaaagagta ctaccgcgag cagggcggga
      901 ccttcagcaa gccgcacctg catgcccagc agtcgggcca ctagcagggt gacggcggcg
      961 ctcagcaggc acagggcggt agtggcgagg atgctcccgg cgaagtaggc ccagggcggc
     1021 agcgggctgc cgcgcagccg cttaagcacg ccccgctcgc gggctgcgac cacggcgccg
     1081 gcgtgcgttg cgtaaccggt catgatgacc ccgaaggcgg tgagcccacc aacgagagca
     1141 gggtctgagc tccctgagta cgaacgcaac aggtggaatg ccacgagcag cagcacgggc
     1201 agaaggaaag ctgagaaggc agcgcgcggc gtgcgggcga gtagcctcaa ctggtaggct
     1261 acttgcccca taagcacatg ccccggtccg ggaagccgct tcaccgtctt ctttgccgag
     1321 ctctcagtcc gccgatcatg caccttcatg atgtctccct tcgttctccc cgtcggcggt
     1381 gagtgccatg taggcgtctt ctagggtgga ctggctcacc gtcagctggt ccaggtttac
     1441 cttccacacc cgcgcccaat cgagcaactc ctcgagtgtc ggcgcaagat cgtcggtcct
     1501 gagggtcgcc caaccgtttt ccagcgctac gtcgcccgct gggagcgtcg gtagcccggc
     1561 cccctccgga accgggaaat ggacgacgct tccgcgtccg gctatctcac tgacccgctc
     1621 cggcggagcg tcggccacta cccggcctcc tgccaccact actactcggt cagcgagcct
     1681 ctcggcctcc tccaggtagt gggtggtcaa cacgacggtc atgccgccgt cgcactgcag
     1741 ttcgatggtc tcccatgccg cccggcgtgc tgcggggtcg aaacccgtgg tcggctcgtc
     1801 cagaaagagc atctccggat tacctacgat cgcgagcccg aggtcaactc gcctcttctg
     1861 ccctcccgaa agtgtaccga tgcgtcgatc cgcctcctcg gcgaggccga ccagctccat
     1921 tacctcctcc accgacctcg ggttcgggta catccccccg taaaggccaa gctcctctct
     1981 gacggtgagc tgctcgtcga gactggtcgc ctggaggacc aaccctaacc gcgctcgcca
     2041 cgagcgcgca gccttctcgg ggaccctccc cgagcacgct cacctgccct ccgtcacgcg
     2101 acctgaatcc ctccaggatc tctaccgtgg tcgtcttgcc cgcgccattt ggcccgagga
     2161 tcgccaccgt ctctcctgcg ccaacttcga agttcacacc cgccagggcc acgttgtcgc
     2221 cataggactt cgtcaacccc gacaccaaga tggcacccat gcttctcctc tctcagcaca
     2281 acttgtgttt tgcggacgat tacgcttggc cgttgcccgt ggagatcttc tattccaaga
     2341 atattggtgc agcgcatcct agccgcgctc aagcacgcgg cgtgcctgag gcaccgaaag
     2401 gaatccctcc ctgagctctt cgtccccgat cctctccgca agctttctca aggtctgcgc
     2461 cgcccgcgag aacgctgctc gcgcctcgct ggtctccctg cgccgctcgt agagcccgcc
     2521 aagacctgcc cggatccgcc acagctcgcc cggcagcccc agcttctcgg cgagcgcctc
     2581 agcctgccgt aagggcaccg ccgccctttc cgggtcgcct tcccactcgc ccaggatcgc
     2641 cagggagcag aggtaggcta ttcgatcgcg ttcgttcgtc tgcgcgcgtt cagcgaaacg
     2701 gcgcacctct tctcgggcga gaagttcgtc ccctccccgc agcagcgcct cgacctcgtg
     2761 gtggaagtgg atgccgagcg gctggttgtt cgcgaagacg tcggcctcga tcgccctcct
     2821 ggcgtgggcg taggcctcct cccagttctc cgagagggcg gccaccacgc acagtctggc
     2881 ggaggaccag gcctcgtact gctggcccag cggctccgcg taggccgccc gcgcctcctc
     2941 caggttgagc aaggcctcgt gagcccgtcc gaggcggtcg agattgaccg ccagcaagta
     3001 cgtgtcttgt gcctcgcggg ccagcccgac accccttctg gcgagcataa gcgcctcctc
     3061 atactcgccg atctcgacaa gtcccaggct aagcgcccat gcgctcatcg cttcgatgcg
     3121 ttccggcaac tctcgggaga tggcatgtgc ctcctgtccg atcgcgagcc cttcttgcag
     3181 cctgccctgg aagatccgtg cgtacgcgag ataggtgaga cactgtatct caatggcctt
     3241 gtggcccgcc ttccaggagg ccgcgagccc tgtgatcgcc gacgtcgccg taggaagtat
     3301 cgtccgtgac ggggggcgtt cggccagctg ctggctcagc gcggcaccct cctcggcgta
     3361 cgccgctgac tccttgagcc ttcccctaaa cacctccact cgtgccagcg cgaccaatgc
     3421 ccgcgctacc aggtccggac gctccagggc ccgggcggag gctagagcct gctgggcgag
     3481 tggcctggaa tgctcggatt cccccaccca gaaagtcatg acatccgcca ggttgcactc
     3541 ggtctcggcg agcgcctcta cgaggcctgc ctcctcggcc accgttcggg cttcctccaa
     3601 gagcgtcctg acgagccgga tgtcgccttg gtggtagtcg aggaccgcca agtgattgag
     3661 ggaaaccacc tcgagcctcg cttcccctag ctcccgccca agagcgagta gcgcctcgta
     3721 ggccgctcgg gatcgactcc actcatcgat catctcgtat gctcgcccaa gctggatgta
     3781 cagatgctcg aggtccggga tcgacggttc ggccagctgc ctaccgccgg ttagtgcttc
     3841 cgcgagcagg ttctgtgccc tctcgtagtg ttcgatggcg tcatgtgtgg caaacacttc
     3901 catagcctga tcacccgccg ccacagaata cgcgaaggcc ggcctcgcaa gaccacccgc
     3961 cagcgcgtga ggcgccagct ccgcgggagg ggcaccactc tcctccagca cctcaagagc
     4021 cctgcggtgc aaaacctgtc gccgcgcgtg gccgctctcg gtgtaggcca cttgccggat
     4081 cttctcgtgc gagaaggagt agacggagct tgggtataag agtggctctt ctgcttcacc
     4141 accggcctcc tcctgtagaa ggtggcgctc gaccagctcg tctagccccc ttaagccctc
     4201 cacctctcct aggcccgcca cgcccacaag cgactcgaag ctgaagctcc tccccaaaac
     4261 ggttccagct ctcaacagct cggaggcggc gggtgagaga tgagacaggc gcgcgcggat
     4321 cacctcgcgc acgttcgatg gtagcaaccc tctcaagtcg ctcccggccc ccaaggccgg
     4381 tccgacctcc acaacgggct ccccgtgcgc acccatctgc attacgagct ctccctcttc
     4441 gagcaaggcc ttcagcgtct ccacgaggta gaagggctgc ccgccggtct ctgcggcgag
     4501 ccattccccg aggctctcca gtttaggttc cgctccgtta gagcccccta gctcctccac
     4561 cgccctcgc
//
LOCUS       DATMJA010000131         4654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_668543, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000131 DATMJA010000000
VERSION     DATMJA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668543"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VNT12_03025"
     CDS             complement(<1..555)
                     /locus_tag="VNT12_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI45119.1"
                     /translation="MGRQRGIEVENLVREFRKGPRAVDGIDLRVEPGEVYGFLGPNGA
                     GKSTTVLMLTTLLPPTSGTATVAGYDIVHEGPQVRASIGAALQEAALDPFLTGREHLR
                     LQAALHGLSRKERKRRGQELLERVGLEDAADRKVRGYSGGMKRRLDLALALVHGPQIL
                     FLDEPTTGLDPQSRSALWAEVSRLA"
     gene            complement(555..1424)
                     /locus_tag="VNT12_03030"
     CDS             complement(555..1424)
                     /locus_tag="VNT12_03030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45120.1"
                     /translation="MDKSNVGMGGKTVLITGGTSGIGKAAAVALAAMGANVVIVGRNQ
                     ERGEATVEEIRARSHNESVELMLADLSVQAEVRGLAEEFQERHDRLDVLANNAGLVQS
                     KRTETPDGIETTLAINHLAPFLLTNLLLGRLEKSAPSRVITVSSEAQRWGTMDFEDMQ
                     FRRKYRGFPVYGMTKLANIMFTYELAERLNATGVSANYLHPGSVGTNFGQNNRGPMAL
                     FFRTFKPFMRSPEQGADTLVWLASSPEVEGGSRKYFSDRKEIEAKSVAYDRAARRRLW
                     EISEELTGLKVAA"
     gene            1595..1795
                     /locus_tag="VNT12_03035"
     CDS             1595..1795
                     /locus_tag="VNT12_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45121.1"
                     /translation="MAPETDPLDVLTIGEMVVDFISVEKIDTLSNHEAASLKRRVEGH
                     VECMIDRQNHYAVLKSAGQQAV"
     gene            1799..2902
                     /locus_tag="VNT12_03040"
     CDS             1799..2902
                     /locus_tag="VNT12_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45122.1"
                     /translation="MAADRKITDLGALISDLSPEDRALANRIFDVSTTTGRLDPPEAM
                     HAWIEKSFGSVDRVTAQRIVKVTNRITLEGTLFNELRASRPLDTGVNLDLDREIAATE
                     GDPFCHPKEGTPADVFGRVEGKHATTASNVAKYDGFHGVIVFDDHNPLHLTPEKVAGY
                     VSVGLEWGRRALEADPEARYPFLMWNCLWRAGGSIIHGHAQVTATRGSHYPKVERLRR
                     NASAYKREHGSDYFEDLHSIHATLGLDIPLQGVHAFASLSPIKEKELVVIGSESGDES
                     LSRAVGALLQGYVEDLGVRAFNVAFYMPPLGPTQEDWADFPTVVYIVDRGDPSNRTSD
                     MGAMELYAASVVASDPFRVAEALLPYSHGGLPG"
     gene            complement(2891..3415)
                     /locus_tag="VNT12_03045"
     CDS             complement(2891..3415)
                     /locus_tag="VNT12_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWI45123.1"
                     /translation="MKFWKIVVVIALLLTSCSGPQADSDTQSQRTPHSSDTTAAGKEE
                     TTSGPRGLRTLVIDASGGEKVEVRVEVADNVFERARGLMYRKALGVNRGMLFVYPEEQ
                     RLSFWMKNTLIPLSIAYIDSKRRIVDIQDMKPLDDEPPSYVSADPAQYALEVNRGFFE
                     ERGVKVGDRVELPG"
     gene            complement(3412..4122)
                     /locus_tag="VNT12_03050"
     CDS             complement(3412..4122)
                     /locus_tag="VNT12_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HWI45124.1"
                     /translation="MTKELFLEDSYTKEFEARVVKLDGREAILDRTAFYPGGGGQPPD
                     KGSLGIGPIEARVVDVRREGGEIIHVMDRPIPDTVWDLKGALQWERRFAHMRYHTALH
                     VLSGVIWRNFDARVTGGQMRADRARMDFSFPGEWTADIVAEIERLTNDALAEERPVRV
                     YELEREKALANPDLIRTQVNLVPERVRRIRIVEIQGLDTQADGGTHVANTREVGRIDI
                     TGHKSKGHQNKRIEFVLQ"
     gene            complement(4119..>4654)
                     /locus_tag="VNT12_03055"
     CDS             complement(4119..>4654)
                     /locus_tag="VNT12_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWI45125.1"
                     /translation="GSEERMENDATALPYDYVIGSVHRVDNQEVDRTTDQSIYDRYET
                     YDLYAAYYANVRKAALSGRFEILGHPDLIKIFRHFPVRDITPILEETADAVAESGIVV
                     DVNAAGLRKPIGEVYPAREFLQMFHKRGVPIVLSSDAHAPDQVALGYDTSLALVHEVG
                     YREVATFRNHERGTLLL"
BASE COUNT          818 a         1555 c         1379 g          902 t
ORIGIN      
        1 cgcgagcctg ctgacctccg cccagagcgc gctgcggctc tgcgggtcga gaccggtcgt
       61 cggctcgtcg aggaagagta tctgtggacc gtgcaccagc gcgagagcga ggtcgaggcg
      121 gcgcttcatc ccgcccgagt aaccgcgtac tttcctgtcc gcagcgtctt cgagcccgac
      181 gcgctccagc agctcctgcc cacgccgctt gcgctccttc cgcgagagtc cgtgcagtgc
      241 ggcctggagg cgaaggtgct cgcggccagt caggaaggga tctagcgccg cctcctgcag
      301 cgctgccccg atagaggccc gcacctgcgg cccctcgtgc acgatgtcgt agccggccac
      361 ggtggccgtg ccgctcgtgg gtggaaggag cgtcgtcaac atcagaacgg tcgtcgactt
      421 cccagcaccg ttcggaccga ggaagccgta gacctcacct ggctccacac gcagatctat
      481 cccatccacg gcccgcggtc ccttcctgaa ctccctgacg aggttctcga cctctatgcc
      541 cctctgccgg cccactaggc ggccaccttc aggcccgtca gttcctcgct gatctcccag
      601 agcctgcgac gggcggcgcg gtcgtaagcc acactcttgg cttcgatctc cttgcggtcg
      661 gagaagtact tcctgctacc accctcgacc tcgggcgagg aagcgagcca gaccagggtg
      721 tcagccccct gctcgggcga gcgcataaac ggcttgaaag tcctgaaaaa gagcgccatg
      781 gggcccctgt tgttctggcc gaaattcgtg cctacggagc caggatgaag gtagttcgca
      841 ctaacgccgg tagcgttcag gcgctccgcg agctcgtagg tgaacatgat attggcgagc
      901 ttggtcatcc cgtagacggg gaagcccctg tacttccgcc tgaactgcat gtcttcgaag
      961 tccatagtcc cccaacgctg cgcctctgag gagaccgtga tcacgcgact cggcgcactc
     1021 ttctcgagcc gcccgagcaa gaggttcgtg agcaggaacg gcgcaagatg gtttatcgcc
     1081 agcgtcgtct cgatgccgtc cggcgtctcg gtccgcttgc tttgaaccag cccggcgttg
     1141 ttggcgagca cgtcgagcct gtcgtggcgc tcctggaact cttcggccaa tcccctgacc
     1201 tcggcctgca cggacaggtc ggccagcatc aactccacag actcgttgtg gctccgggct
     1261 cttatctcct cgactgtcgc ctcgccgcgc tcctggttgc gaccgacaat gacgacgttc
     1321 gcgcccatgg ctgccagcgc caccgccgcc gccttcccga taccggaggt gccacccgtg
     1381 atgaggaccg ttttaccgcc cataccgacg ttcgacttat ccaaaacctc tcctgcacca
     1441 gaaacctcgc ctcaccctga gtctataccg ctttcgcgta cgcgcaccgt gagcggagcc
     1501 acgcgctgcc tgcccgtccc agttgctctg tagggttccc gatcccttca cgagggtgga
     1561 cgcggggccc gaaggttaaa tagactggat gcctatggcg ccagagacag accccctcga
     1621 cgttctcacc atcggggaga tggtcgtcga tttcatctca gtcgagaaga tcgatactct
     1681 gagtaaccac gaggcagcga gcctgaaacg tagggtcgaa ggtcacgtgg agtgtatgat
     1741 cgaccgccag aaccactacg cagtcttgaa gtccgccgga cagcaggccg tgtagcagat
     1801 ggcggccgat cgcaagataa ctgaccttgg ggccctgatc tccgacctct ccccggaaga
     1861 ccgcgccctc gcaaaccgca tcttcgacgt ctccacgacg acgggacgcc tcgatccgcc
     1921 tgaggcgatg cacgcctgga tcgagaagtc cttcggctcg gtggaccgcg tcaccgccca
     1981 gcgtatcgtg aaggtcacca accgcatcac cctggaaggc acgctcttca acgagttgcg
     2041 tgcctcccgc ccactcgaca cgggcgtcaa cctggacctc gaccgggaga tagcagcgac
     2101 ggaaggcgac ccattctgcc acccaaagga aggcacgccg gccgacgtct tcggccgtgt
     2161 cgaaggcaag cacgcgacta ccgcctcgaa cgtcgccaag tacgatggct tccacggcgt
     2221 catcgtcttc gatgaccaca acccccttca cctcacgccc gagaaggtcg cgggctacgt
     2281 ctcagtcggc ctggaatggg gtcgcagggc cctcgaagcc gacccggagg cgcgctaccc
     2341 tttcttgatg tggaactgtc tctggcgggc tggcggatcc atcatccacg gccacgcaca
     2401 ggtcacggca acccgaggct cccactaccc gaaggtcgaa cgcctccgcc gcaacgcttc
     2461 ggcctacaag cgggagcacg gctccgacta cttcgaggac ctccacagca tccacgcaac
     2521 tctcggcctg gacatcccgc tgcaaggcgt ccacgcgttc gcgagcctct cccccatcaa
     2581 ggagaaggag ctggtcgtga tcgggagcga gtccggcgac gaatctctga gccgcgcggt
     2641 cggcgccctg ttgcaaggct acgtcgagga cctcggcgtc cgcgccttca acgtcgcctt
     2701 ctacatgccc ccgctcggtc ctacacagga ggactgggca gatttcccga cggtcgtcta
     2761 catcgtggat cggggcgacc cgtccaaccg tacctcggat atgggggcga tggagctcta
     2821 cgcagcctcc gtcgtcgctt ctgacccgtt cagggtggcc gaagccctgc tcccctactc
     2881 ccacgggggc ctacccgggt aactcgacgc ggtcccctac cttcacgccc ctttcctcga
     2941 agaagccccg gtttacttcg agagcatact gggcggggtc ggcggagacg tagctcggcg
     3001 gttcatcgtc aaggggtttc atgtcctgga tgtctacgat acgcctcttc gagtcgatgt
     3061 aggcgatgga gagcggtatc agggtgttct tcatccagaa agagagcctc tgctcttccg
     3121 ggtagacgaa gagcatgccc ctgttcaccc ctagcgcctt cctgtacatg agacctcgcg
     3181 cccgctcgaa aacattgtcg gccacctcga ctcgcacctc gaccttctcc cctcccgaag
     3241 cgtcgataac cagcgtgcgg agcccccggg gccccgaggt cgtctcttcc ttccccgcgg
     3301 cggtagtgtc agaagaatgc ggggtcctct gtgattgtgt gtcagagtcg gcctggggac
     3361 cactacagct agtcaggagc agggcgatga cgacaacgat cttccagaat ttcactggag
     3421 cacgaactcg atccgtttgt tctggtggcc cttgctcttg tggcctgtga tgtctatcct
     3481 cccgacctcg cgtgtgttgg cgacgtgagt cccgccgtcg gcctgcgtgt cgaggccctg
     3541 tatctccacg atccgaatac gccgcacccg ctcgggcacc aggttgacct gggtacggat
     3601 gaggtcgggg ttggcgagcg ctttttcgcg ctccagctcg tagaccctta caggacgttc
     3661 ttcggcgaga gcgtcgttgg tcaggcgttc gatctcagcg acgatgtcgg cggtccactc
     3721 gccgggaaaa gagaaatcca tcctggcgcg gtccgcgcgc atctggccgc ctgttaccct
     3781 ggcgtcgaag ttgcgccaga tcacacctga gaggacgtgc agcgcagtgt ggtatcgcat
     3841 gtgggcgaat cgccgctccc attgcagcgc acccttcaga tcccacacag tatcagggat
     3901 gggtctgtcc atgacatgga tgatctctcc accctctcgc ctcacgtcca cgacccttgc
     3961 ctcgatggga ccgatgccaa gggacccctt gtcaggcggc tggcctccgc cgccaggata
     4021 gaaggccgtg cggtcaagta tcgcttcacg gccatctagc tttacgacgc gcgcttcgaa
     4081 ctcctttgta taggagtctt ccaggaagag ctccttggtc atagcagtag ggttccccgc
     4141 tcgtggttcc tgaaggtggc gacctcgcgg tagcccacct cgtgtacgag cgcgaggctc
     4201 gtgtcgtagc ccaatgcgac ctgatccggc gcgtgggcat ccgaggagag aacgatagga
     4261 acgcctctct tgtggaacat ctggaggaat tctctggcag gatagacctc gcctatgggc
     4321 ttcctgagcc cagcggcgtt cacgtcgacg acgataccag attcagcgac ggcgtcagcg
     4381 gtctcctcca ggatcggcgt gatgtcccta acgggaaagt ggcggaagat ctttatgagg
     4441 tcagggtgac cgaggatctc gaaccgtcct gagagcgcgg ccttgcggac gttcgcgtag
     4501 tacgccgcgt agaggtcgta ggtctcgtat ctgtcgtaga tactctggtc cgtggtcctg
     4561 tccacctcct ggttgtccac cctgtgtacg ctgccgatga cgtaatcata agggagggcc
     4621 gtggcgtcgt tttccatgcg ttcctcggag cccg
//
LOCUS       DATMJA010000132         2589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_431678, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000132 DATMJA010000000
VERSION     DATMJA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431678"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1031)
                     /locus_tag="VNT12_03060"
     CDS             complement(201..1031)
                     /locus_tag="VNT12_03060"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWI45126.1"
                     /translation="MNGLSPKTIQGLRSPLARIVEVHEALGSTQERARELAHAGKPHG
                     TLVVAEIQTGGRGRLRRHWGSPKGGLWMSLVLRPWFDASLAPRITQTAAVGVAKALWE
                     VGVEASIKWPNDVLAGGKKICGILAEASAGYATGPARERYLDYVILGVGMNANLDPAE
                     LGVPDREVTTIRSELGRDADLLELLRVVLSNLDAELGRIEDFGGVLEDWRNLNCTLGE
                     RVRVLRLGETIEGKAVDLTAEGALLLVTRRGTVELFEGEVEHLRQGDEMGGLLDSDGS
                     "
     gene            complement(1028..2089)
                     /gene="cobT"
                     /locus_tag="VNT12_03065"
     CDS             complement(1028..2089)
                     /gene="cobT"
                     /locus_tag="VNT12_03065"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWI45127.1"
                     /translation="MGLEDRLLELAAEVPPLDDAAVEEARRRQLWLTKPPGSLGRLEE
                     VGARLAGMAGRCPPPVPESPAVVVCAGDHGVLERGVSPWPQAVTAAMVSSFCTGGAAV
                     NAIAKTVGARVSVLDVGVAGELERHPLLRAAKVRRGTGDLSRGPAMSREEAARAVMAG
                     AGVAEELVESGGVDLLVTGDMGIGNTTPAAALISAFTGHPPEETTGRGTGIDDQTLKL
                     KVNVVKEALELHVPDPEDPLGVLAKIGGLEHAAIVGVILAGAVYGLPVILDGVVTDSA
                     AFVARAFAPHSAQYVIAGHRSTEPGARISLQSLEVRPLLDLDMRLGEGTGGLLAVPLV
                     QAAARTLKEMAMLEEIGFG"
     gene            complement(2092..>2589)
                     /locus_tag="VNT12_03070"
     CDS             complement(2092..>2589)
                     /locus_tag="VNT12_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HWI45128.1"
                     /translation="EEKEETAVLIVGRGSSDPDANSDLCKIARLYYEGRHYPVVESAY
                     VSMTPPDVANGLDRCLKLGARHVVVFSYFLFTGVLEERIRGQSRAFAEANPGVEVRYA
                     GYIGPDPRLADLVVERYTEALRGDIRMNCDVCVHRVALPGFEEKVGAPVTPHYHPDEP
                     GHHHH"
BASE COUNT          452 a          887 c          787 g          463 t
ORIGIN      
        1 cttatagggg accagatagc cgtaaccggg gacgtggaga tcgtagattt ccccgagagg
       61 ggtaagatcc tggtcttcaa ggccacctcg gtggcagtcc tcgaagaaga cgaagagatg
      121 tccacctagt ttgcacgggt ccagaccagc cagtccggga acttgctaac tggctgagat
      181 cctgaggcct gtagccccat tcaagaccca tcggagtcga gcaggccgcc catttcgtct
      241 ccttgcctga gatgttcgac ctcgccctcg aacaactcca cggtgccccg tcgggtaacg
      301 agcaatagag cgccctctgc agtcaggtcc acagccttgc cttcaatggt ctcgccgaga
      361 cggaggacac gcaccctctc cccaagggta cagttcaagt tgcgccagtc ctcgagtacg
      421 cctccgaagt cctcgatcct acccagctca gcgtcgaggt tcgagagcac aacgcgcagc
      481 agttccagca ggtcagcgtc acggccgagc tcggaacgga tcgtggtgac ctcgcggtca
      541 gggaccccga gctcggccgg gtcgaggttg gcattcatcc ccaccccgag gatgacgtaa
      601 tcgaggtacc tctctcttgc cggtcctgtg gcgtaccccg cgctagcttc ggcgaggatg
      661 ccgcagatct tcttcccacc agcgagcaca tcgttgggcc acttgatgct ggcctcgacc
      721 ccgacttccc agagcgcctt cgcaaccccg acagccgcag tctgggtgat gcggggggct
      781 aggcttgcgt cgaaccaggg acgcagcaca agtgacatcc agagcccgcc cttcggagat
      841 ccccaatggc gtcggagcct cccccgtccc ccggtctgga tctccgcgac gacgagcgtt
      901 ccgtgaggtt ttccggcatg ggctagctcg cgggcgcgct cctgggtcga gcccagcgct
      961 tcgtgtacct cgacgatacg ggccaacggg ctacgcaatc cctgaatcgt tttcggagag
     1021 agaccgttca cccgaatcct atctcctcga gcatcgccat ctccttaagc gtccgggccg
     1081 ctgcctgcac gagcggcaca gcgagcaggc ctcctgttcc ttcgcccaga cgcatatcca
     1141 ggtctagtag tggacgcact tcgagcgatt ggagggagat acgcgcccct ggctcggtgg
     1201 agcgatggcc tgcgatgaca tattgcgccg agtgcggagc gaaggcgcgg gctacgaacg
     1261 cggcagagtc cgttactacg ccgtcgagga tgactgggag gccgtacacg gcgccagcca
     1321 ggatcacacc gacgattgca gcgtgctcta gaccaccgat cttcgcaagg accccgagag
     1381 ggtcttctgg gtctggcacg tgcagctcca gcgcctcctt cactacgtta accttgagct
     1441 tgagggtctg gtcgtcgatg cccgtgccgc ggcccgtggt ctcctcaggc gggtgtcctg
     1501 tgaaggcgga gatcagggcc gctgccggcg tggtgttccc gatgcccatg tcgcccgtta
     1561 ccaggagatc caccccaccg ctctcgacga gttcttccgc gactcctgcc cccgccatca
     1621 cagcccgtgc tgcctcctca cggctcatcg caggcccgcg actcaggtcg ccggtcccgc
     1681 gccgcacctt cgcggcccgc agcagagggt gcctctccaa ctccccggca accccgacgt
     1741 cgagaacact caccctcgcc cccaccgtct tcgcgatggc gttcaccgcc gccccacctg
     1801 tgcagaagga actcaccatc gccgccgtaa ccgcctgagg ccacggcgag acgccgcgtt
     1861 cgaggactcc gtggtccccg gcgcagacaa ccacggcagg actctcaggg acgggcggcg
     1921 ggcaccggcc ggccatccca gcgagccgcg caccgacctc ctccaaccgt cccaggctac
     1981 ctggcggttt ggtgagccag agttgtcgcc ttcgcgcttc ttcgactgcg gcgtcgtcga
     2041 gtggcggtac ctccgctgcg agctccagta agcggtcttc aaggcccacg gctagtgatg
     2101 gtggtgcccc ggctcgtcgg ggtggtagtg gggcgttacc ggggcgccga ccttctcctc
     2161 gaagccaggc agggcgacgc ggtggacgca aacgtcgcag ttcatgcgaa tgtcccctct
     2221 cagggcctct gtataacgct cgaccacaag gtcagccaac ctcgggtcag gcccgatgta
     2281 ccccgcgtag cgcacctcga cgccgggatt cgcctcagca aatgctctgc tctgcccgcg
     2341 gatacgctcc tcgaggacgc cggtaaagag gaagtaggag aagaccacga cgtgcctggc
     2401 gccgagcttg aggcagcgat ccagcccgtt cgcgacatcg ggcggggtca tgctgacgta
     2461 agcactttcg actacggggt aatggcgtcc ttcatagtac agacgcgcta tcttgcagag
     2521 gtctgagttg gcgtcggggt cggaggagcc gcgaccgacg atgaggactg ctgtctcctc
     2581 cttctcctc
//
LOCUS       DATMJA010000133         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_550199, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000133 DATMJA010000000
VERSION     DATMJA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550199"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VNT12_03075"
     CDS             <1..1549
                     /locus_tag="VNT12_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWI45129.1"
                     /translation="GGMGEVYLARDRVLGRDVALKVLRKQYAGDDEFAERFKREAMSA
                     ASLSHSNIIQVYDRGKTEDGAPYIAMEYVPGGTLKERISREGPLEAADAAALGAQVAE
                     ALGAAHDRGMVHRDIKPQNVLLAARGEAKVADFGIARAGSSATISRTGSVMGTAGYMS
                     PEQALGKPATPKSDLYSLGVVLYEALTGELPFNADNPIAVSMKHVNEPLRPPIELNPR
                     IPKGMNALVTKLLAKDPEDRYADADELADDLWRLNRGLEPVLAGVGRKQGPGTQRTAL
                     TSPNVTSEMQRSRPAPVSFRRRRRLPWLLIAILFTLGVLGSVQAILGPDAMKGWFDVF
                     QGEVQSSGQPAGPEKVKVPEVDGLTRSMAEKRLTDAGLEVGKVTSFPSNSVAAGRVIE
                     PGIVAGTPVESGTDVNLTISSGPQPSSSASSSASAQSSLEPGASASASSSASAQPAQR
                     QDRQAVQTPAAEANGPKANVPRAKVQKSPPAQKQNAAAPAKEPVDDSGGQGRGRGRGH
                     GGGPGKD"
     gene            1779..2750
                     /locus_tag="VNT12_03080"
     CDS             1779..2750
                     /locus_tag="VNT12_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWI45130.1"
                     /translation="MKAIRIHEFGGPEVLSYEDVEIPEPGRGQARVKLAASGVNFIDV
                     YQRTGRYPGDLPRTLGLEGAGEVDAVGESVDDVSPGDYVAFASTPGSYAEYVVAPAEN
                     LAPFNATLVEARVAAAAMLQGMTAHYLTHSTFPLQEGQTALVHAAAGGVGLLLVQIAK
                     MRGATVIGTAGTEEKVKLAREAGADEVILYTEQDFVEETNRITEGEGVHVVYDSVGKD
                     TFDGSLDTLRPRGYMVLFGASSGPVPPFDPQVLNQKGGLFLTRPALAHYTATREEFLW
                     RAESILSWIGQNNLDVRIGGTYSLEDAEQAHRDLEGRRTTGKLLLIP"
     gene            2760..>3315
                     /locus_tag="VNT12_03085"
     CDS             2760..>3315
                     /locus_tag="VNT12_03085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWI45131.1"
                     /translation="MKLDAGLGTEGDYLKGMDRTARAAEDLGFSGLWTSETKHDAFLP
                     LAIAANETHEIELGTSVAIAFSRSPMEMAQTAWDLQDLSGGRFVLGLGTQVKAHITRR
                     ISMPWGRPAARLREYILALRAIWESFQSEGPLNFEGDFYRHTLMTPFFNPGPIEHPEI
                     PIYIAGVNTRLANLAGEICDGFHVH"
BASE COUNT          690 a         1064 c         1052 g          509 t
ORIGIN      
        1 cggaggcatg ggcgaggtct acctggcccg cgacagggtg ctcgggcgcg acgtggcgtt
       61 gaaggtgctt aggaaacagt acgccggcga cgatgagttc gccgagcgct tcaagcgcga
      121 ggccatgagc gcggcgtccc tctcccactc gaacatcatc caggtctacg acaggggcaa
      181 gacggaagat ggggcccctt acatagccat ggaatatgtg cctggcggta cgctcaaaga
      241 gaggatctcc agggagggcc cgctcgaggc ggcggacgcc gccgcgctcg gagcgcaggt
      301 cgccgaggcg ctcggagcgg cccacgacag aggcatggtc caccgcgaca taaagcccca
      361 gaacgtacta ctcgcagcca ggggcgaggc gaaggtggcg gacttcggca tcgccagggc
      421 cggctcctcc gcgaccatct ccaggacggg gtccgtgatg ggcaccgccg gctacatgtc
      481 tcccgaacag gcccttggta agcccgcgac tcccaagagc gacctctact ccctcggagt
      541 ggtgctctac gaagcgctca caggcgaatt gccgttcaat gccgacaacc cgatcgccgt
      601 ctccatgaag cacgttaacg agccgttacg gccccccata gaactaaatc cccggatacc
      661 caagggcatg aacgcgctcg tgacgaagct cctggcgaaa gacccggaag accgctacgc
      721 ggacgccgac gagcttgcgg acgacctctg gaggctgaac cgtggactcg agccagtctt
      781 ggcgggtgtc ggaaggaagc agggaccggg cacgcaacgt actgccctca catcgccgaa
      841 cgtcacgagc gagatgcaga gatcccggcc agctccggta tcgtttcgac gcaggcgacg
      901 cctgccctgg ttgctgatcg cgatcttgtt taccctcggg gtgctcggca gcgtccaggc
      961 tattctgggt ccggatgcca tgaaaggctg gttcgacgtc ttccagggag aagtgcaatc
     1021 ttctggccag cccgctggac cggaaaaggt gaaggtaccc gaagtggacg ggctaaccag
     1081 gagcatggca gagaagaggc tcaccgacgc ggggctcgag gtcgggaagg tcacttcgtt
     1141 tccgagcaat agtgtcgccg caggcagggt aatcgagcca ggcatcgttg caggtacccc
     1201 agtcgagtca ggcacagacg tcaacctgac catcagctca ggaccacaac cttcctcatc
     1261 cgcgagctcg tccgcgtctg cccagtcgag tctcgaaccc ggggcttccg cctccgcaag
     1321 ctcttccgcg tcggcgcagc ccgcccaacg ccaggatcgc caggcggtcc agacgccggc
     1381 agccgaggcg aatggtccca aggccaacgt acccagagcc aaggtgcaaa agagtccacc
     1441 tgcacaaaag cagaatgcgg ctgcaccggc aaaggaacct gttgatgaca gcggggggca
     1501 aggtcgcggt cgaggacgcg ggcacggtgg aggacccggg aaggattaga gcgattggca
     1561 ggggcatcga cgtgctgcga atcacgcgtc cgcctacgag cgataagcta caggcgaaaa
     1621 caacatactg accagccgaa atgctggcaa gcggaggcct gaaaggccga agcgtgctga
     1681 aatgctgact agcgcccgac aaggcgcatg ctgaattgct gaaatgctgc cacaatgacg
     1741 ggcgtgtaag tcgagttcat cgcgagaggg agagaagcat gaaggcgatc cggatacacg
     1801 agttcggtgg acccgaggtc ctcagctacg aggacgtcga gattcccgaa ccgggccgtg
     1861 gccaggcgcg tgtcaagctt gcggcctcgg gcgtcaactt tatagacgtc taccagcgta
     1921 cgggccgtta cccgggcgac ctgccccgta cgctcgggct ggaaggcgcc ggcgaggttg
     1981 acgctgtggg tgaaagcgtc gatgacgtgt cgccgggaga ctacgttgcg ttcgcgagca
     2041 ccccgggatc ctacgctgag tacgtagtcg cccccgccga gaacctggcg cctttcaacg
     2101 ccacgctcgt cgaagcgcgg gtcgccgcgg ccgccatgct ccagggcatg acagcgcact
     2161 acctgacgca cagcaccttc ccgctacagg aaggccagac ggcgctcgtg cacgccgcag
     2221 caggtggcgt tgggcttctg ctcgtccaga tcgcgaagat gcggggggcg accgtgatcg
     2281 ggaccgcagg caccgaggaa aaggtgaagc tcgccaggga ggcaggggcc gacgaggtca
     2341 tcctatacac cgagcaggac ttcgtggagg agacgaaccg tatcacggaa ggagaaggcg
     2401 tccacgtcgt ctacgactcg gtaggaaagg acaccttcga cggtagcctc gacaccttgc
     2461 gcccgcgcgg ctacatggtg ctcttcgggg catccagcgg tcccgtgccc ccgttcgacc
     2521 cccaggtcct caaccagaaa ggtggcctct tcctcacccg tcccgccctt gcccattaca
     2581 ccgcgacccg cgaggagttc ctgtggcgcg ccgagagcat cctatcctgg atcggacaga
     2641 acaacctcga cgtccgcatc ggcggcacct actctctcga agacgcagag caggcccacc
     2701 gcgacctcga aggccgcagg accacgggca agttattact tatcccgtag gacgataccg
     2761 tgaagctcga cgcaggcctc gggacagagg gagattacct gaaaggtatg gacaggaccg
     2821 cccgagcggc cgaagacctt ggcttctccg gcctgtggac cagcgagacc aaacacgacg
     2881 ccttcctgcc ccttgccata gccgcgaacg aaacccatga gatagagctg ggtacctctg
     2941 tagccatagc cttctcgcgc tcaccgatgg agatggccca gacggcctgg gacctccagg
     3001 atctctccgg tggacgcttc gtgctaggcc taggcacgca ggtaaaggca cacataaccc
     3061 gccgcatctc gatgccctgg ggcaggccag ccgcgcggct gcgcgagtac atcctggccc
     3121 tgcgcgcgat ctgggagtct ttccagtcag aggggccttt gaactttgag ggcgacttct
     3181 accgccacac cctcatgacg ccgttcttca accctggccc tattgagcac ccagagatcc
     3241 cgatctacat cgcaggcgtc aacacccgcc tcgccaacct cgccggggag atctgcgacg
     3301 gcttccacgt gcacc
//
LOCUS       DATMJA010000134         4307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_136305, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000134 DATMJA010000000
VERSION     DATMJA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136305"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VNT12_03090"
     CDS             <1..453
                     /locus_tag="VNT12_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45132.1"
                     /translation="RDTHVMKHWHPMFVEPALGYFGLSFFGHDHTRHVHKVEDGAFVR
                     APITSCWNYDFLERDCDGPDHYKAFLTDGSTRDSMIGAYKGDLGSNSPPRAKACAVNT
                     NPYREFCVSDYRDDGKVVWRETHHNLDSHTLAVGCPGPRLELPCRSRD"
     gene            525..788
                     /locus_tag="VNT12_03095"
     CDS             525..788
                     /locus_tag="VNT12_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45133.1"
                     /translation="MRCAALLRQTAGDDPRNKILVLDVPGWEGHKVGQHVDVKLTAED
                     GYQAQRSYSIASAPEDERLTVDRLDEGEGSSALGRKIGAAMTG"
     gene            957..1082
                     /locus_tag="VNT12_03100"
     CDS             957..1082
                     /locus_tag="VNT12_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45134.1"
                     /translation="MDDAKRGMDKRRLEAVTSFLDHRGIETGHLAARREVVCPAS"
     gene            1067..1528
                     /locus_tag="VNT12_03105"
     CDS             1067..1528
                     /locus_tag="VNT12_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWI45135.1"
                     /translation="MPGFVEGIYVARGAGAPVERVEEVAAIEGCGLAGDRYCIGTGHW
                     SRFGRACQVTLIAAEDLDAMEKETGIRVRDGEHRRNVVLRGVSLGDLRRRRFRIGGAT
                     LEYVGPRSVCRYIERLTEPGMTQALKGRGGVCVRVVESGPVRVHDQVDVLP"
     gene            1584..2078
                     /locus_tag="VNT12_03110"
     CDS             1584..2078
                     /locus_tag="VNT12_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45136.1"
                     /translation="MFASNLGGRVRSYALRSIAYIDGRVIVASLIVSCDNLTIGVAPD
                     EADPLIERLQALKYLGRHRPSDYVSADHDEVGIDALEIYQDSLKRWQVGVYIVDGCNT
                     RALLNHTCGMILLFAKPRESGALGSCTVPRASAVLLVNSDVCTGALFIAAPSAPRTGL
                     WRFL"
     gene            complement(2122..2736)
                     /locus_tag="VNT12_03115"
     CDS             complement(2122..2736)
                     /locus_tag="VNT12_03115"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI45137.1"
                     /translation="MSGRRAEAARNDGLILEAAREVFVADPKAPISAVSERAGVGIGA
                     LYRRYGSKEDLLRRLCADGLGIYIAAVEEALSDEGDPWKAFTGFMRRVVDADTHSLTL
                     RLAGTFTPTEELYRDSQRAQELNVRLFERTRAAGAIRPEVEVDDIALLLEQLAAVRVA
                     DEERTKQLRHRYLALLLDAIHTPAESQLPGPPPSWEEIEQRWTT"
     gene            2844..3032
                     /locus_tag="VNT12_03120"
     CDS             2844..3032
                     /locus_tag="VNT12_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HWI45138.1"
                     /translation="MSKMSDSGAEIRAFRIDIPEQDLDDLRDRLGRTRWTNELPLDTS
                     GPQTGPVPPGWEYGVPVS"
     gene            complement(3398..>4307)
                     /locus_tag="VNT12_03125"
     CDS             complement(3398..>4307)
                     /locus_tag="VNT12_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45139.1"
                     /translation="LDGRVQALQESYIRKVVDTVQDLPNVLYEVANESSGGGSVEPEF
                     LEAIGLSEPPDWGDSTQWQYWVIDFVKQYEAQMGYARHPVGMTMQYPVRDQSKVNDVL
                     FDSPADWISPGFDDVDSPMQDSRWSTNPPANNGAKVIISDTDHYSPMKSDALWAWKSF
                     LRGHNPILYDLGIVTGVNPPDPSSGSPSFASLEPARYAMGDTLRFAREMDLVAMEPRG
                     DLSSTGYTVANPGEEYLVLQPDEKADPFTARLEANTYSVRWHSVGSRETKDADKVTVE
                     KHGNTTFTAPFAKAGPAVLYLKGGGR"
BASE COUNT          805 a         1315 c         1375 g          812 t
ORIGIN      
        1 agggacaccc acgttatgaa gcactggcat cctatgtttg tggagcctgc cctgggctac
       61 ttcgggctgt cgttctttgg gcacgaccat acccgccatg tgcacaaagt agaggacggc
      121 gctttcgtgc gtgcgccgat aacgagctgc tggaactacg attttctcga acgcgactgc
      181 gacggcccgg accactacaa ggcgttcctg acagacggca gtacccgcga ctccatgata
      241 ggcgcctaca aaggcgatct aggctccaac tctccaccac gggccaaggc gtgcgccgtc
      301 aacaccaacc cctaccgcga attctgcgta tcggactatc gggacgatgg taaggtagta
      361 tggcgcgaga cgcaccataa cctagattct cacactctcg ctgtagggtg tccgggtccc
      421 cggcttgagc taccgtgtag gagcagggat tgagtaaaaa ctcccgacat ggctagtcgt
      481 cataggctac acgcttttgg cagttgagtt gctgatggtg ttgtatgcgc tgtgcggcgt
      541 tactccgcca gacggcgggg gatgacccga ggaacaagat tttggtgctc gacgttcccg
      601 gatgggaggg gcacaaggtc ggccagcacg tcgatgtcaa gctcaccgcc gaggacggct
      661 accaggccca gaggagctac tccatagcct ctgccccgga ggacgagagg cttacggtcg
      721 accgtctgga tgaaggcgaa ggttcgtctg ctctaggccg caagattgga gcggctatga
      781 caggctaatt gatgccgaga tgctagcgga ggtgacgtgg ttcccagatg agagtcctct
      841 cgccttcgtg tgcggaccga ccccgctcgt ggaggcggtg gggtccgcgc tggtcagcct
      901 tgggcacgac cccgcacaag tcaggaccga acgcttcaga gcgacggtag gttagaatgg
      961 acgatgccaa acgcggcatg gacaagcggc ggctcgaggc cgtcacgagc ttcctcgatc
     1021 accggggcat agagaccgga cacctcgccg cgaggaggga ggtagtatgc ccggcttcgt
     1081 agaggggatc tacgtcgcgc gcggagctgg cgcgccggtg gaacgcgtgg aggaggtcgc
     1141 ggcgatcgaa ggctgcgggc tagcaggcga ccgctattgc ataggcaccg gacactggtc
     1201 ccgcttcggc agggcatgtc aggtcaccct gatagcggca gaggacctcg acgccatgga
     1261 gaaggagacg ggcatccgcg tccgcgacgg cgagcaccgg cgcaacgtcg tcctccgcgg
     1321 cgtaagcctc ggggacctgc gccggaggcg gttccggatc ggcggggcga ccctcgagta
     1381 cgtggggccg cgctcggtct gccgttacat tgagaggttg accgagcctg gaatgacaca
     1441 ggcgctcaaa ggacgcggcg gcgtctgtgt cagagttgta gagagcggtc ctgttcgcgt
     1501 ccatgaccag gtcgacgtgc tcccctgatc cgagcttcac accgcaagac gaaagactcg
     1561 gagaaggagg ccagcaagtc tctgtgttcg cgtctaatct tggaggcagg gtaagaagct
     1621 acgcgctccg cagcatcgcc tacatcgatg gcagagtcat cgtagcgtct ctcatcgtaa
     1681 gctgtgacaa cctcacgatc ggcgttgccc cggacgaagc agatccgctc atcgagcgcc
     1741 tccaggcgct caagtacctc ggcaggcatc ggcccagcga ctacgtctcc gccgaccacg
     1801 atgaggtcgg gatcgacgct ctcgagatct accaggatag cctcaagcgc tggcaggttg
     1861 gcgtgtatat cgtagatggc tgcaacacgc gagctcttct caaccatacc tgcggcatga
     1921 tactcctgtt cgcaaaacct cgggaaagtg gtgctcttgg aagctgcact gtgccaaggg
     1981 cttcggcagt gctcctggtc aatagcgacg tttgtacggg tgccttgttc atcgcggcgc
     2041 cttcggcgcc gcggactgga ctttggcgct ttctatgatg gtgctcccct tccgtacgtc
     2101 ccatgggatc acgagagagg gctacgtagt ccaacgctgt tcgatctcct cccagctcgg
     2161 tggcgggccg ggtagttgag actctgccgg agtgtggatg gcgtccagca gcagcgcaag
     2221 gtatcggtgg cgaagctgtt tggtgcgctc ttcatccgct acccgtacag ccgctagttg
     2281 ctccagcagc aaggctatgt cgtccacttc gacttcgggg cggatggcac ccgcggccct
     2341 ggtgcgctcg aacaaacgca cgttgagctc ctgtgccctc tggctgtccc ggtacagttc
     2401 ctccgtgggc gtgaaggttc cggccaggcg cagcgtcagc gagtgggtgt cagcatccac
     2461 gacgcggcgc atgaagcccg tgaaggcttt ccagggatcg ccttcgtcag acaaggcttc
     2521 ctccacggcg gcgatgtaga tcccgagacc gtcggcacag agccgcctca agaggtcctc
     2581 cttgctccca tagcggcggt acagggcacc gatgcccacc ccagcccgct cggaaacggc
     2641 agagatcggg gctttcgggt cggcgacgaa aacctcgcgg gcggcctcca ggatcaagcc
     2701 gtcgttgcgc gccgcctcag cccgccgacc actcattgac ccggaagtat tttctctcct
     2761 catacgccaa ttatatatgg aacaaaacat tccgttcaag tataatggag cggaacggtt
     2821 tgttccgaag gaaggagttg tctatgtcga agatgagcga ctcgggcgcc gagatccggg
     2881 cgttccggat cgacatccca gagcaagacc tcgacgatct acgcgaccgt ctgggccgca
     2941 cacgctggac caacgagttg ccgctcgaca cgagcgggcc ccagaccggt ccggtcccgc
     3001 cgggttggga gtatggtgta ccggtctcct aggtcaagga gctggtcgag tactggagag
     3061 acggctatga gtggcgcgcg tgggaggcga agctgaacgc ctacccgcag ttcaccacca
     3121 ccatcgacgg gcaggacgtc cacttcctcc acgcccgctc acctgagccc ggtgcgctcc
     3181 cgctaatcct cacccacggc tggcccaaca cggttgtgga gtacctggag ctggactttg
     3241 gccaggtgcg caccctaccg accacaatga tacaagcgct ggcccgatcg aaggccatga
     3301 aggccaccat cttcacgact agggccaggg tgggcggccg tcccagtacg gtaccgcgct
     3361 cgccagagac cccggacctc cgcacattag caatctgtta gcgtccgcct cctttcaggt
     3421 agaggaccgc cgggccggcc ttcgcgaacg gggccgtgaa ggtggtattg ccgtgctttt
     3481 cgaccgtcac cttgtcggcg tctttcgtct cgcggctgcc gacgctatgc caccggaccg
     3541 agtaggtgtt ggcttccagc ctcgcggtga acgggtcagc cttttcgtcg ggctggagga
     3601 cgaggtactc ttcgccgggg ttcgcgaccg tgtatccggt cgagctgaga tccccccggg
     3661 gctccatcgc gacgagatcc atctcccgcg cgaagcgcag cgtatcgccc atcgcgtagc
     3721 gggccggttc cagggacgcg aaggaggggc tgcccgagga ggggtcgggc gggttcacgc
     3781 cggtgacgat cccaaggtcg tacaggatcg ggttgtggcc ccggaggaat gacttccagg
     3841 cccacagcgc gtcgcttttc atgggcgagt agtggtcggt gtcgctgatg atgaccttcg
     3901 caccgttgtt tgcgggcgga ttcgtggacc accggctgtc ttgcatcggc gaatccacat
     3961 cgtcgaagcc cggggaaatc cagtccgccg ggctgtcaaa cagcacgtcg ttgactttgc
     4021 tctgatcccg gacggggtac tgcattgtca tccccacagg gtgcctagcg taccccatct
     4081 gtgcctcgta ctgcttgaca aaatcgatga cccaatactg ccactgcgtg gagtcgcccc
     4141 aatcgggtgg ctcagacagt cctatcgcct cgaggaattc cggttcgacg gaaccgccgc
     4201 ccgaggactc gttggcgacc tcgtagagga cgttgggcag gtcctggaca gtgtcgacaa
     4261 ctttgcggat gtagctctct tgcaaagcct gcacgcgccc atcgagc
//
LOCUS       DATMJA010000135         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_432026, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000135 DATMJA010000000
VERSION     DATMJA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432026"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VNT12_03130"
     CDS             complement(<1..263)
                     /locus_tag="VNT12_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45140.1"
                     /translation="MYSVLLILILLGALISLVTAFRALGAYLRFRRTRAAFQDKVTDE
                     IARLSARTDELEISLSALDARATELPIQISELQQSLTTLQVLT"
     gene            336..409
                     /locus_tag="VNT12_03135"
     tRNA            336..409
                     /locus_tag="VNT12_03135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:370..372,aa:Met,seq:cat)
     gene            600..1067
                     /locus_tag="VNT12_03140"
     CDS             600..1067
                     /locus_tag="VNT12_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HWI45141.1"
                     /translation="MSEGSGGSPLKGEMGNTTISSTVVAQIAGIAAQEVEKVQMGGGT
                     SAAVGGFLQSVAGGVTGGSTSGGDYSRGVSVEVGEEEAAVDLAMTIEYGQSIPQITEA
                     ARRNVINRVESLTGLRVTEVNIAVTDVQIPEERPMLQEQEQTEEMARRQEQRT"
     gene            1212..2498
                     /gene="tilS"
                     /locus_tag="VNT12_03145"
     CDS             1212..2498
                     /gene="tilS"
                     /locus_tag="VNT12_03145"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWI45142.1"
                     /translation="MDLRSPLALVSGGPDSVALLRVVVALGGGPVVLHVDHGLRGEES
                     REDAEFVRELCRRLNVRCEVRRLGLDGGSNLQERARDERYRLAEEAAVRLGLRVVATG
                     HTADDVAETVLMNLARGTGLRGLAGIPPVRGNVQRPLIGCTRREILDYLKELDQPYRT
                     DPTNLTPKYARNRVRLDVLPILEELYPAAAGNIARAASLVREDLEILEELATGTVEMR
                     GEEVVLPLDGLVQLRPPLRRHAVRLAYTTVVPDTAPLPSNLVEAVLGLLEGGEGTRTL
                     DLPGGVVASGRSGEELALYRKPRSMVSDREKIRAGEAMVFGGWRISAREVSGYDPVDA
                     ARPEVAYLDAGKEPYEVRMAREGDIIRPLGLGGTKRVLRAMMDRKVPSDLRRSTPVVV
                     DGRDEVAWIMLGELDERYKVDERTEKILRLEVARIS"
     gene            2501..3052
                     /gene="hpt"
                     /locus_tag="VNT12_03150"
     CDS             2501..3052
                     /gene="hpt"
                     /locus_tag="VNT12_03150"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWI45143.1"
                     /translation="MSSMMPDVKEVLITSQEIEEKVREIGARITEDYRGQNPLLIGVL
                     RGAVVVMSDLMRNIDLQCELDFMDISSYGSGTSSSGVVRILKDLEEDITDRHVLIVED
                     IIDTGLTLSYLRRSLLARKPASLEICALLSKPSRRRTDLDVRYLGFEVPDEFVVGYGL
                     DYAGTYRNLPDIYILKPEVFATD"
BASE COUNT          618 a          864 c         1060 g          548 t
ORIGIN      
        1 ctcgtcagaa cctgtagcgt cgtcaggctt tgctggagtt cagagatctg gatgggaagc
       61 tccgtggcgc gggcgtctag ggcggagagg ctgatctcca gctcatccgt tcttgcggag
      121 aggcgggcga tctcgtctgt aaccttgtcc tggaaggccg cgcgggtacg ccgaaacctc
      181 aggtaagccc cgagtgcacg gaaggcggtg accagcgaga tgagggcccc caggagtatc
      241 aggatcagta gaaccgaata catgtcgatg gcaacggtac cacggcgctc cctgccttta
      301 caaactccgg gccggtgtta gaatctcgcc tacgacgcgg ggtggagcag tctggtagct
      361 cgtcgggctc ataacccgaa ggtcgcaggt tcaaatcctg cccccgctac ttctccgagg
      421 ccccgtaccc gcggggtttt gcctttcctg gacgtattcc cgagagtttg tacccgtcgt
      481 acgcgtggga aggtgcggag tttaggattt tgaacagcgg ggtaggcagc ctagataacg
      541 gtgcgtagtg gcgcgcagat ctcacccctg gtggaacgaa cgaatcggaa aggacgagca
      601 tgagcgaagg aagcggcgga agcccactaa agggcgagat gggcaacact acgatcagca
      661 gcacggtggt cgcccagata gcgggcatcg cggcgcaaga ggtagagaag gtgcagatgg
      721 gcggcggcac ctccgcggcc gtcggcgggt tcctgcagag cgtggccggc ggtgtcacag
      781 gtggcagcac gagcggcggt gactactcca ggggcgtgag cgtcgaggtc ggcgaggaag
      841 aggccgccgt ggacctggcg atgaccatag agtatggtca gtcgataccc cagatcacag
      901 aggccgcgcg gcgcaatgtg atcaaccgcg tggagagcct cactggcctg cgtgtgaccg
      961 aggtaaacat agcggtcacc gacgtccaga tccctgaaga gcggcccatg cttcaagagc
     1021 aagagcagac agaagagatg gccaggcgcc aggagcagcg aacctgagct agggcactac
     1081 gccttaggat ggaagaccgg ggcaccacgc cccggtcttt tcttttgcga gcccctgcac
     1141 ttcactaaac tgtctgcatg ggatccgcga gtttaccgga taaggtgcat cggactgcgg
     1201 agcgttacgg catggacctc cgaagcccgc ttgcgctggt ctccggcggt ccagactcgg
     1261 tggcgctgct gcgggtggtc gtcgcactcg gtggcggacc ggttgtgctg cacgtggacc
     1321 acggactgcg cggcgaggaa tcgcgcgaag acgcggagtt cgttcgcgaa ctatgtcgta
     1381 ggttgaacgt acgctgcgaa gtacggcgac tcgggctcga cggtggttcc aacctccagg
     1441 aacgggcgcg tgatgagagg tacaggctcg ccgaggaggc ggccgtccgg ctgggactga
     1501 gggtcgtagc gacgggccac actgccgacg atgtggccga gaccgtgctc atgaacttgg
     1561 cacggggcac aggcttgcgc gggctcgccg gcatcccgcc tgtacggggt aacgtccagc
     1621 gtcccctcat cggatgcacg cgaagggaga ttctggatta cctgaaagag ctggaccaac
     1681 cctatcgtac ggaccctaca aacctgaccc ccaagtacgc ccgcaaccgc gtccgtctcg
     1741 atgttctgcc tatccttgag gaactgtatc cggcggcggc aggcaacatc gcgcgcgccg
     1801 cctccctcgt ccgtgaggac ctcgaaatcc tcgaagagct cgcgaccgga acagtggaga
     1861 tgaggggaga ggaggtggtc ctgccgctcg acggactcgt gcaattgcgg cctccgctcc
     1921 ggcgccacgc cgtacgcctg gcatacacga ccgtggtccc ggataccgcg ccgctccctt
     1981 ccaatctggt cgaagcggtg ctgggacttc tggaaggtgg tgagggtaca cggacgttgg
     2041 acctgccggg tggggtagtc gcctcgggcc gctccgggga ggagctcgcg ctctacagaa
     2101 agccgcggtc gatggtttcg gaccgagaga agatcagggc cggggaggcg atggtgttcg
     2161 gggggtggag aatctcggcg cgggaggtct ctggttacga tcctgtagat gcggcacgtc
     2221 ctgaggtggc ctacctcgac gctggaaagg agccgtacga ggtgcggatg gcgcgggagg
     2281 gggatattat ccggccgttg ggcctcggtg ggaccaagag ggtcttgcgg gcaatgatgg
     2341 acaggaaggt gccgagcgat ctaaggcgca gtacgcccgt cgtggtggac gggcgggatg
     2401 aggtcgcctg gataatgctc ggtgagctcg acgagcggta caaggtggat gagaggacgg
     2461 agaagattct caggttggag gtggcaagaa tctcgtgagt atgtccagta tgatgcccga
     2521 cgtgaaagag gtcctcataa cctcacagga gatagaggag aaggtgcgtg agataggggc
     2581 gcgcatcacc gaggattaca ggggccagaa tccgctccta atcggtgtct tgcgcggcgc
     2641 agtcgtggtg atgagcgacc tcatgcgcaa catagacttg cagtgcgagc tggatttcat
     2701 ggacattagc tcctatggca gtggcacctc atcgagcggc gtcgtccgca tcctcaagga
     2761 tctcgaagag gacatcacag accgtcacgt ccttatcgtg gaggacatca tcgacacggg
     2821 tctgacgctc tcgtacctga ggcgttccct gctcgcccgc aagccagcct cgctcgagat
     2881 ttgcgcccta ctgtccaagc cctccaggcg gcgaacagac ctcgacgtca gatatctcgg
     2941 cttcgaggtg cccgacgagt tcgtcgtcgg atacgggctc gactacgcgg ggacctaccg
     3001 gaatctcccc gacatctaca tactcaagcc cgaggtcttc gcgaccgact aagagaaaga
     3061 gcgtcctatt cgatacgcag gtagtataat
//
LOCUS       DATMJA010000136         7298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_728538, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000136 DATMJA010000000
VERSION     DATMJA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728538"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VNT12_03155"
     CDS             complement(<1..206)
                     /locus_tag="VNT12_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HWI45144.1"
                     /translation="MQTEGVDARRAVDELKELAELTGGPDGARRVAWTAEWEKAREWF
                     RGKLEEIEGITEIETDEAGNVWATA"
     gene            complement(206..1519)
                     /locus_tag="VNT12_03160"
     CDS             complement(206..1519)
                     /locus_tag="VNT12_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWI45145.1"
                     /translation="MTNPDELLRRHKAALPSWTPLYYEKPLQLARGEGFRVWDSEGNE
                     YLDFFGGIVTTISGHAVPEITEAIKSQVDKILHTSTLYLIENQVRLAEKLIDLAPISG
                     EKKAFFVGSGSEANEAALLFATRYRKSSEIIALRSSYHGSTFMTMGITGQSTWSPTSR
                     SALDVAYARNPYRLRCALCRDEGRCNLLCADDVRAVVEEETTGNVAAFIAEPIQGVGG
                     FVEAPPGYFERVKEILDETGILLISDEVQTGFGRTGEHFWGIDAFDVEPDMIVMAKGL
                     GNGLAIGAVVGRAEVVESVAPNLHLSTFGGNPISTAGALANIDYILDNDLQTNAYEVG
                     THLKDRLLRIAEDHAIVAEVRGRGLMLALELAGEDLAPDPAAAIRFLEACRSRGLLVG
                     KGGLKANTIRISPPLVITREAADEAADIMEEALAEVGAATPEKVG"
     gene            complement(1516..2922)
                     /gene="hydA"
                     /locus_tag="VNT12_03165"
     CDS             complement(1516..2922)
                     /gene="hydA"
                     /locus_tag="VNT12_03165"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWI45146.1"
                     /translation="MSGLLFSGGTVVSAEGSYRADVLVEDEKIFAVGTNLDTEDADTV
                     DASGKLVMPGFIDAHTHMDMPFGGTVTADDWATGTEAAAAGGTTMIIDFALQEAGGTL
                     AGAFDTWTEKAQGKAVIDYGFHVAITDLRDDIKTELPDLAEKGVASVKIFMAYKGTPL
                     HTEDDDLFEVLQLSRDAGVIVLVHAENGHVIARLQEQALSRGDTAPRFHALSRPEPVE
                     AEATNRAIRLAEVAGAPILVVHVSCAPALEVIHLAHERGQTVYAETCPQYFAFEYEDL
                     AREGFEGAKYVCSPPLRDPSSRPALWNGLKFGDLQIFGSDHCSFNYREQKELGRDDFT
                     LIPNGLPGAEERAMTLWTLGVREGKLSENQFVAVLSTNQARIYGAYPRKGTLAPGADA
                     DIVLWDPELSTTATVENRHGKVDYTPYEGMEFHGGPAAVYVRGGLVYKDGEVVGERGS
                     GQFIQRSFTATEGLEVRV"
     gene            complement(3005..4168)
                     /locus_tag="VNT12_03170"
     CDS             complement(3005..4168)
                     /locus_tag="VNT12_03170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI45147.1"
                     /translation="MEPGSEGTLNGGDDADRRGGALKVAVIGTGIVGSSVAYRLSGGG
                     AEVVMIDGAEPGSGTTSTSFAWVNANNKLPRNYFELNVAGMREHERLRDEIGGDWLYS
                     TGNLIWAVDEEQENLEKRVERLRSWSYAAEMLPASTVNEQLEPGAAFSDPAVRIAHFP
                     EESWTDAPALTRTLVEAATRNGAHEHFGNAVRGIEVDGAGVMLQLQEGDAVHADVVVN
                     ATGVKAASVAEMVGRKLPLDVFPGLLIRVAVSDEPLRHLMHTPRINVRPDGPGYVLLH
                     HDSVDERLTDDFAGTEDPLCTELLERARLILPALEEAMVVEARFGMRPVPADGHSCVG
                     ALSGVPGYYEAVTHSGVTLGPLIGRLLAREILAGEVDPLIAPFRPDRFPATQT"
     gene            complement(4428..5372)
                     /locus_tag="VNT12_03175"
     CDS             complement(4428..5372)
                     /locus_tag="VNT12_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI45148.1"
                     /translation="MGWRNGGYASVLLVAVGAAMWGTDGILRVPLLEVASPSQIVLLE
                     HLVLLLYSVPAVVLGWRFFRGLGAAQWVALLVIGWGGSALATLLFTTAFAVGNPTVVI
                     LLQKSQPLFAIALAAILLRERLGWAYWPCFAVAMVGAYMISFGNLGPFTALGSAELLP
                     AVLALGAALLWGSSTVLGRLVLKDVPFYALTGARLFLAVPLLAGIVVAQSSVGGLAAG
                     FASEPGRVILLALIPGLLALLLYYRGLTGTRASYATLAELAFPATAVVLNWTFLGIGV
                     SANQLLGFVLLWGAVFMLGYLNARAPAPDPSAPVVGTG"
     gene            complement(5376..6380)
                     /locus_tag="VNT12_03180"
     CDS             complement(5376..6380)
                     /locus_tag="VNT12_03180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWI45149.1"
                     /translation="MKPTGTVGYAAMFEQFHPTDLLNWSKTAEESGFGSVMASDHFHL
                     WTPEQGQSAFVWSWMGALGATTNLRFGTGVTPPGFRYHPAIIAQGAATLEAMYPGRFW
                     LGLGAGEALNEHIVGDYWPEAPTRLRILMEAIEVIRRLFTGKVVKYSGEHTKLESARL
                     YTMPDTPPPLYVATAGPIQSRRTGEFCDGIITVGAPDEKIKNLMEKFEEGARRADKDP
                     SEMPRMIQLHVSWAEDQDEAEQQAVREWPNGGMPFPKADIRNPEDFAAMAKQVSVENF
                     KNRVLMSADLDEHLEHIQHYVDLGFDEVYVHNVGRNQEEFIKAYGERVIPKVDWPEKG
                     "
     gene            complement(6591..>7298)
                     /locus_tag="VNT12_03185"
     CDS             complement(6591..>7298)
                     /locus_tag="VNT12_03185"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HWI45150.1"
                     /translation="EEDFSSLMDLYEAEAKGHTLSVRRSEGHWKSSLSKKDADVAVYE
                     RDGDMEGYILYKISGWQDRDPRRTLSVEEIVAATPRAREALLSFMAGLDPLVYGIKLY
                     TPRGEPLHPYLRSSHVNAKIEPDQMLRLVDVEGALGYLERAPEMPLVLDISDDVIAEN
                     AGEYTVAEGNIIRGAEAAESVSLDVRQLAQLYAGYLPAGDLARHGLIRASSPEALDLL
                     GSLFPLRDPWLFGPDHF"
BASE COUNT         1275 a         2372 c         2290 g         1361 t
ORIGIN      
        1 gccgtagccc agacgttgcc ggcctcgtcg gtctctatct cggttatgcc ttcaatctcc
       61 tccagcttgc ccctgaacca ctctcgggcc ttctcccact ccgcggtcca cgcgacgcgg
      121 cgcgccccat ccgggccgcc ggtaagctcc gcgagctcct tgagttcgtc tacggcgcgg
      181 cgggcgtcca ctccttcggt ctgcatcagc cgaccttctc gggggtggcg gccccgacct
      241 cagccagggc ctcctccatg atgtctgcgg cctcgtcggc cgcttcgcgg gtgatgacga
      301 gcggcgggga gatgcggatg gtgttcgcct tgaggccgcc cttgccgact agcaggccgc
      361 gggagcgaca ggcttcgagg aagcggatgg ctgcagccgg gtccggtgcg aggtcctccc
      421 cggcgagctc cagggctagc atcaggcccc ttccgcggac ctcggcgacg atcgcgtggt
      481 cttcggcaat cctcaggagg cggtccttta ggtgggtacc gacctcgtaa gcgttcgtct
      541 ggaggtcgtt gtccaggatg tagtcgatgt tcgccagggc gcctgcggtc gagatcgggt
      601 tcccgccgaa ggtggagagg tggaggttcg gggcgacgga ctcgactacc tcggccctgc
      661 caacgacggc acctatggcg aggccgttgc cgagaccctt ggccatcacg atcatgtctg
      721 gctccacgtc gaaggcgtcg atgccccaga agtgctcgcc cgtgcgcccg aagcccgtct
      781 ggacctcgtc ggagatcaag agtatccccg tctcgtccag gatctccttt accctttcga
      841 agtagccggg cggcgcctcg acgaaacctc cgacgccctg gataggctcg gcgataaacg
      901 ccgctacgtt gcccgtggtc tcctcttcta cgacggcgcg cacgtcgtcc gcgcaaagga
      961 ggttgcagcg cccctcgtct cggcacaggg cgcagcggag cctgtaaggg ttgcgggcgt
     1021 aggccacatc tagggccgag cgcgaggtcg ggctccaggt actctggccc gtaatgccca
     1081 tcgtcatgaa tgtagagccg tggtaggaag aacggagggc gatgatctcg ctggacttgc
     1141 ggtagcgcgt cgcgaaaagg agcgcggcct cgttcgcctc cgagccgctg cccacgaaga
     1201 aagccttctt ctcgccggag atgggggcga gatctataag cttctcggcg agccttacct
     1261 ggttttcgat caggtacagc gtcgaggtgt gaaggatttt gtccacctgg ctctttatgg
     1321 cctcggtgat ctcaggcacg gcgtgcccgc ttatagtggt gacgatccct ccgaagaagt
     1381 ccaggtactc gttcccctcg gaatcccaca ccctgaaacc ctcaccgcga gcgagctgga
     1441 gcggcttctc gtagtacagc ggcgtccacg aaggcagcgc cgccttgtgc cgccgcaata
     1501 gctcatccgg gttcgtcata cccgcacctc cagtccctct gtcgccgtaa agctccgctg
     1561 gataaactga cccgagcccc gctcgccgac gacctcgccg tccttgtaca ccagcccgcc
     1621 acgcacgtac accgccgccg ggccgccatg aaactccatc ccctcgtaag gcgtgtagtc
     1681 cactttgccg tgacggttct cgaccgtcgc cgtcgtcgag agctcggggt cccacagcac
     1741 gatgtccgcg tcggcgccgg gggccagggt tccctttctc gggtacgcgc cgtagatgcg
     1801 cgcctggttg gtcgagagga cggcgacaaa ctggttctcg gagagtttcc cctcgcggac
     1861 gccgagcgtc cagagggtca tggcgcgttc ttcggcgcct ggtagtccgt tgggtatcag
     1921 ggtgaagtca tccctgccaa gctctttctg ctcgcgatag ttgaaagagc agtgatccga
     1981 cccgaagatc tggaggtccc cgaacttgag gccgttccac aacgcgggcc tgctgctcgg
     2041 gtcgcgcaac ggtggggagc agacgtactt ggcgccctcg aaaccctcac gggcgagatc
     2101 ctcgtactcg aaggcgaagt actggggaca ggtctcggca tagaccgtct ggccgcgctc
     2161 atgcgccaaa tgaatgacct cgagggcggg cgcgcacgag acgtggacga ccagtatcgg
     2221 tgctccggcg acctcggcga gccgtatcgc cctgttcgtc gcctcggcct cgacgggctc
     2281 gggacggctc agggcgtgga agcggggggc cgtatcgccc cgcgagagcg cctgctcctg
     2341 gagcctggct ataacgtgcc cgttctccgc gtggaccagt acgatgacac cggcatcccg
     2401 ggagagttgc agtacctcga agaggtcgtc gtcctccgtg tgtaggggcg ttcccttgta
     2461 tgccatgaag atcttgaccg aagcaacgcc cttctcggca agatcaggca actcggtctt
     2521 tatgtcgtcg cgcagatccg tgatggcaac gtggaaaccg taatcaatga ccgccttgcc
     2581 ctgcgccttc tcggtccacg tgtcgaaggc tccggcgagg gtgcctccgg cctcctgcag
     2641 ggcgaagtcg atgatcatgg tcgtaccgcc cgccgcagcc gcctctgtgc ccgtggccca
     2701 gtcgtcggcc gtaacggtac ccccgaacgg catatccatg tgggtatggg catcgatgaa
     2761 gcccggcatt accagcttgc cagaggcgtc gacggtatcg gcgtcttcag tgtcgaggtt
     2821 cgtgccgacc gcgaatatct tctcgtcctc gacgagcacg tcggcccggt aactgccctc
     2881 ggccgagacg acggtcccgc cagagaaaag aagcccgctc atagtgttct ccttccactt
     2941 agcagcggtg tgttggctaa ctctcgcaca ccccgcacct tcacgtcaat cgtaaagtag
     3001 agggctacgt ttgcgttgcc gggaagcgat ccggacggaa cggtgcgatg agcgggtcca
     3061 cctcgccggc caggatctca cgcgccagca gacgcccgat gagcgggccg agcgtgacgc
     3121 cgctgtgcgt cacagcctcg tagtagcctg ggactccgga gagcgcgccg acgcaagaat
     3181 gtccgtccgc cggaaccgga cgcataccga agcgcgcctc tacgaccatc gcctcttcta
     3241 gcgccggaag tatgagccgc gcccgttcca gaagctccgt gcaaagcgga tcctccgttc
     3301 cggcgaagtc gtctgtgagc ctttcgtcaa cggagtcgtg atgcagcagg acgtatccgg
     3361 gaccgtcggg gcgaacattg atgcgcggtg tgtgcatgag atgtctcaga ggttcgtcag
     3421 agacggcgac ccggatgagt agtcctggga acacgtcgag cggcagcttt ctcccgacca
     3481 tctccgccac ggatgcggct ttcacgcccg tggcgttgac cacgacatcg gcgtgaacag
     3541 cgtcgccttc ctgcagttgc agcatcactc ctgcgccatc cacctcgata ccgcgcaccg
     3601 cattcccgaa atgttcgtgt gcaccgttgc gggtggcggc ttccacgagc gttctcgtca
     3661 gggccggagc atcggtccag gattcttcgg ggaagtgggc gatccggacc gcggggtcag
     3721 agaaggcggc tcccggttcg agctgctcgt ttactgtgga ggcggggagc atctcggcgg
     3781 cgtaggacca ggagcgcaga cgttcgacgc gtttttcgag gttttcctgt tcttcgtcca
     3841 cggcccagat caggtttccc gtagagtaga gccagtctcc gccgatctca tcgcggagcc
     3901 gttcgtgttc gcgcatcccg gccacgttca gctcgaagta gttgcgggga agcttgttgt
     3961 tggcgttgac ccaggcgaag ctggtcgagg tcgtgcctga acccggttcc gccccgtcga
     4021 tcatgacaac ctccgccccg cccccagaga gccggtaggc gacagaagag ccgacgatcc
     4081 cggttccgat gacggcgacc ttcaacgcgc ctccacgccg atcagcatcg tctcctccat
     4141 tcagagttcc ttcagatcca ggttccaaag tcactctcct ccgtcttagc taccatcgcc
     4201 acacggccgc gtattcggga aacaccacaa atcctgacgg agcggggcgt attctgccat
     4261 aggtcgggcg tacaatacga catcagagca ggcccgttgg atcggggagc agcccatgtt
     4321 cttcgtcgcc tccgccgtcc cggacgcgca cgtcaacgtc tcactccagg gctgggtcca
     4381 aaacacccgt agagacagga gaggcgcgca gtcctggtac gaagcggtta cccggtgccc
     4441 accaccgggg cggacgggtc aggcgccggg gctcgggcgt tcaggtagcc aagcatgaat
     4501 acggctcccc agaggaggac gaagccaagg agctggtttg cactcacgcc gatcccgagg
     4561 aacgtccagt tgaggacgac cgccgttgcc gggaaggcca actccgcgag cgtcgcgtag
     4621 gaggccctgg tgccggtgag gccccggtag tacaggagca gggccaggag gccagggatc
     4681 agggcgagga ggatcacccg gcccggctcg gaggcgaacc cggcggcgag cccgcccacg
     4741 gagctctggg cgactacgat gccagcgagc aggggcacgg ccaggaagag ccgcgcccct
     4801 gtcagggcgt aaaacggcac atccttgagg acgagcctcc cgagcacggt cgaggagccc
     4861 cacaggagcg ccgctcccag ggcgaggacg gcgggtagga gttcggcgga gccaagggcg
     4921 gtgaaaggac cgaggttgcc gaaggagatc atgtacgccc ccaccatcgc taccgcgaag
     4981 cacggccagt acgcccatcc cagcctctcg cgcaacaaga tggccgccag cgcaatggcg
     5041 aagagcggct ggctcttctg caacaggatc accacggtcg gattgcccac ggcgaacgcc
     5101 gtcgtgaaga gcagcgtcgc cagggccgat ccgccccacc caatgacgag cagcgcgacc
     5161 cactgcgccg ccccgaggcc gcggaagaat ctccagccca acaccacggc cggcaccgaa
     5221 tacagaagca aaaccaggtg ctccaggagc actatctgag acggcgacgc cacctccaac
     5281 aagggcaccc gcaagatgcc gtccgtcccc cacatcgccg ccccgacggc gacgagcagg
     5341 acgctggcgt agcctccgtt cctccagccc atgcgctatc ccttctccgg ccagtcgacc
     5401 ttggggatga cgcgttcgcc gtaggctttt atgaactcct cctggttgcg gcctacgttg
     5461 tggacgtaga cttcatcgaa gccaaggtct acgtagtgct ggatgtgctc gaggtgctcg
     5521 tcgaggtcgg cgctcatcag gacccgattc ttgaagttct caacgctgac ctgctttgcc
     5581 atcgctgcga agtcctcggg gttccttatg tccgcctttg ggaagggcat cccgccgttg
     5641 ggccactccc tgacggcctg ctgctcggct tcatcttgat cctcggccca ggagacgtgg
     5701 agctgtatca tgcgcggcat ctcggagggg tccttgtcgg ccctgcgcgc cccctcctcg
     5761 aacttctcca tcaggttctt tatcttctca tccggggcgc cgacggtgat gatgccgtcg
     5821 cagaactcgc ccgtcctgcg gctctgtata gggcccgctg tcgcgacgta gagcgggggc
     5881 ggagtatctg gcatggtgta gagcctcgcg ctctcgagct ttgtatgctc gccgctgtac
     5941 ttgacgacct tccccgtgaa gagcctgcgt atgacctcga tggcctccat gaggatgcga
     6001 agcctggtcg gcgcctcggg ccagtaatcg ccgacgatgt gctcgttcaa cgcctcgccc
     6061 gcgccgaggc cgagccagaa gcgtccaggg tacatagctt ccagcgtcgc cgctccctgg
     6121 gcgattatgg ccggatggta ccgaaagccc ggcggggtca cgcccgtgcc gaaacggagg
     6181 ttcgtcgtcg cgcccaaggc gcccatccag gaccatacga aggcgctctg accctgctct
     6241 ggggtccaca ggtggaagtg gtccgaggcc ataacggagc cgaaccccga ttcttcagcc
     6301 gtcttactcc aattcagaag atccgtggga tggaactgct cgaacatcgc cgcgtagcca
     6361 acggtgcccg tgggtttcat ggttgtgcct ccttcgtcgg cagcatttca gccagtcagc
     6421 atttcggctg gtcagccact cttcggacgg agcattcgcc actccgccta aggctagttt
     6481 acagtgggac aaacatttgc cgctctgaac gtgccgctgt ccaccaccaa gctgaagtgt
     6541 tgacaagcga aggccgcagg ccgtagcgtg ctgaccggct gaaatgctaa tcaaaaatga
     6601 tccggcccaa agagccatgg atctcgcagg gggaaaaggc tccccaggag atcgagagct
     6661 tcgggagaac tcgcccggat gagcccatgc ctggcaaggt ccccggccgg aaggtacccg
     6721 gcgtagagct gggcgagctg acgtacatcg agggagacgc tctccgccgc ttccgccccg
     6781 cggataatgt taccttctgc gacggtgtac tcgccggcgt tctctgctat tacgtcgtct
     6841 gaaatgtcaa ggactagcgg catctctggc gcgcgctcaa ggtaccccaa ggcgccttcg
     6901 acgtcgacga ggcgcagcat ctggtctggc tcgatcttcg cgtttacgtg cgagcttctc
     6961 aggtaggggt gaagcggctc tccgcgcggg gtgtatagct ttataccgta gaccagggga
     7021 tcgaggcccg ccataaagga cagaagtgcc tctctcgccc gcggtgttgc ggcgacgatc
     7081 tcctctaccg agagggttcg tcgtgggtcg cggtcttgcc acccggagat cttgtagagg
     7141 atgtagcctt ccatatcacc gtcccgctcg tagacggcga cgtccgcgtc tttcttggac
     7201 agactcgatt tccagtgtcc ttcgctcctg cgtacgctga gggtatgtcc tttcgcctcc
     7261 gcttcgtaga gatccatcaa agaggagaag tcttcttc
//
LOCUS       DATMJA010000137         4696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_76532, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000137 DATMJA010000000
VERSION     DATMJA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76532"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VNT12_03190"
     CDS             <1..644
                     /locus_tag="VNT12_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HWI45151.1"
                     /translation="EAHYGLPEVFGATAGRLVANPGCYPTAAILALAPVVKRMGAGIR
                     SVTINALSGVSGAGAKPSAKTHFVSVNESAGAYGAPRHRHTPEIETILRRVGEAPEVT
                     FVPHLIPITRGELETIVVDVGDVPQVEEVLGWYAEDYEGWAFIEAREEYPHVAHVANT
                     NRCRLSAAVDGRAGKLLLFSAVDNLLKGASGAAVQNMNLALGYEEYLGLEHLT"
     gene            655..1134
                     /locus_tag="VNT12_03195"
     CDS             655..1134
                     /locus_tag="VNT12_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45152.1"
                     /translation="MGATVSPVDKTTRQDLIVRLISERDLGTQQDVVGALADEGVEVA
                     QATVSRDLAELGVLKVGNRYLALPHDPGSAGIEVLPSFVLGLVPAQNQVVIHTRDGTA
                     GAVSSVLDRVKGLKILGTIAGQDTVLAIAPDVAAATQVANLIADVCRAKTRPAGNVE"
     gene            1223..2449
                     /locus_tag="VNT12_03200"
     CDS             1223..2449
                     /locus_tag="VNT12_03200"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWI45153.1"
                     /translation="MLEGKKVVLAYSGGLDTSVCVKWLEQQGAKPYALYLDLGQGEPS
                     EDVKAKALEIGATDAFVRDARAEFAREYVAPAIMANALYGGKYPLFTALGRPLIAKKL
                     VEAAREVGATLIAHGSTGKGNDQVRFDVTTASIAPDLTVVAPVRDWNMSRPEEMAYAE
                     EHGIPVPTTKESPYSVDENLWGRSIEAGPLEDPDHEPTEAVFALTTSPETAPDEPQYV
                     ELGFESGLPKTLDGQELPLVDLISELGAVAGANGVGRIDMLEDRLVGIKSREIYEAPA
                     ALAIIAAHRELETLTLTRDVLRFKTTVEQRYAELTYEGLWFTPLKSALDAFVAETQKT
                     VTGTVRLKLYKGNSTIVGRRAPNALYSEELATYDPNSTFDEAAAAGFIALWGLSARQW
                     ASVNGGAEYSQTSSQH"
     gene            2742..3590
                     /gene="arsM"
                     /locus_tag="VNT12_03205"
     CDS             2742..3590
                     /gene="arsM"
                     /locus_tag="VNT12_03205"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HWI45154.1"
                     /translation="MAEDLKQRVRDRYAGAARSVLGEAEDGGSCCGGGASCCGVVESG
                     ERAAFEVDMACGSYSEAERSELPQLAADASLGCGNPVALATLSPGEVVLDLGSGGGID
                     VLLSARRVAPGGRAYGLDMTDEMLELARRNQAEAGVENAEFFKGEIEDVPLPDGHVDV
                     VISNCVINLSTEKQRVIGESFRVLKPGGRFAVSDVVFFGEKNKLPPGIAQSVELWSGC
                     VSGALEKDEYEALLSEAGFEEVVVEVTHTYPPEQIAGLSGEDAEALREVPAASAFIRA
                     RKPEAL"
     gene            3593..4009
                     /locus_tag="VNT12_03210"
     CDS             3593..4009
                     /locus_tag="VNT12_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWI45155.1"
                     /translation="MTQRQLPVLQESSVASGTIDIGGEERAGRLVALGRALSDPIRVQ
                     MLGMLAEGRGCCELPDCGVPARDQDAGICVCEFERYFDAGQSKVSYHLGKLKEAGLVH
                     EERRGKWSFYSLDRTAAGQLLAETAAHLDGRPGNDG"
     gene            4002..4442
                     /locus_tag="VNT12_03215"
     CDS             4002..4442
                     /locus_tag="VNT12_03215"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWI45156.1"
                     /translation="MADRRAKDSTRVLFVCTHNSARSQMAEGLLRGMAGDRFEVFSAG
                     TEATRVRPEAVSVMAEIGVDISAQESKTLDRYLGETLDLVITVCDDANEACPVFPGAR
                     ERLHWSFPDPSKAAGGHEERLRAFRYVRDEIRARIEGELAKPGV"
     gene            4506..>4696
                     /locus_tag="VNT12_03220"
     CDS             4506..>4696
                     /locus_tag="VNT12_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HWI45157.1"
                     /translation="MTAPLTHDPSETDLAMMRRALLAAEESAEKGEVPVGAVVARGDE
                     ILAVAANEREATGDPTAHA"
BASE COUNT          914 a         1395 c         1560 g          827 t
ORIGIN      
        1 ctgaggcgca ctacgggttg cctgaggtct tcggggctac ggcggggagg ctagtcgcca
       61 accccggttg ctatccgacg gccgctattc tggctcttgc tcctgtggtg aagcgtatgg
      121 gggctgggat acgctccgtt accatcaacg ctctttccgg ggttagcggg gcaggggcga
      181 agccgagcgc aaagacgcac ttcgtcagcg tgaacgagag tgcaggcgct tatggggcgc
      241 cgcggcacag gcacacgcct gagatcgaga cgattctgcg gcgtgtgggg gaggcgcccg
      301 aggtgacgtt cgtaccgcac ctcatcccca taacgcgcgg ggagctagag accatcgtgg
      361 tcgacgtcgg ggacgtgcct caggtggagg aggtcctcgg ctggtacgcg gaggactacg
      421 aggggtgggc tttcatcgag gcgcgggagg agtatccgca cgtcgcgcac gtagcgaaca
      481 ccaacaggtg caggctctcg gctgcggtag acggccgggc agggaagcta ttgctcttct
      541 cggcggtcga caacctgctc aagggcgcct cgggtgcggc ggtacagaac atgaacctgg
      601 cgctcggcta cgaggagtac ctcgggctcg agcatcttac gtaggaggac tgttttggga
      661 gctacggtgt cgccggtgga caagacgacg aggcaggatc tgatagtgcg tttgatctcg
      721 gagcgcgacc tcggcaccca gcaggatgtc gtcggcgcgc tcgcggacga gggggtcgag
      781 gtcgcgcagg cgaccgtgag ccgcgacctc gccgagctgg gggtccttaa ggtcgggaac
      841 cgctacctgg cgctgccgca cgaccccggc tccgcaggta tcgaggtcct ccccagcttc
      901 gtcctcggcc tcgtgccagc gcagaaccag gtcgtcatcc atacccgcga cgggaccgca
      961 ggggcggtct ccagcgtcct cgacagggtg aaaggcctga aaatacttgg caccatcgcg
     1021 ggccaggaca ccgtgctcgc catagcccca gacgtagccg cagccacaca ggtggcgaac
     1081 ctcatcgccg acgtctgccg ggcgaagacg cgtccagccg gcaacgtcga gtagtcgaag
     1141 gccggacctt cggggtgtgc ggataccact gcgtctggat ccgagctcac cctgacccca
     1201 tagatacgtt tcaggaggaa tcatgttgga aggaaagaag gtcgtactcg cctattcggg
     1261 cggccttgac acctcggtct gcgtcaagtg gctagagcag cagggggcaa agccttacgc
     1321 cctgtatctg gatctcggcc agggagagcc ttcggaggat gtaaaggcga aagcgctgga
     1381 gataggcgcc acagacgcct tcgtacggga cgcgagggca gagttcgcca gggagtacgt
     1441 tgcccctgcg ataatggcga acgcgctgta cggcgggaag tacccgctct tcacggcgct
     1501 cgggcgccca ctcatagcga agaagctcgt ggaggctgcg cgggaggttg gggcgacgct
     1561 catcgcgcac ggctcgacgg ggaagggtaa cgatcaggtc cgcttcgacg tgacgacagc
     1621 ctccatcgcc ccagacctga ccgtcgtcgc ccccgttcgc gactggaaca tgagccgtcc
     1681 cgaggagatg gcctacgccg aggagcacgg catccccgtc cccacaacga aagaatcccc
     1741 ctacagcgtg gacgagaatc tctggggccg ctctatcgaa gcagggcccc tggaagaccc
     1801 cgaccacgaa cccaccgagg ccgtcttcgc gctcaccact tctcccgaga ccgcccccga
     1861 cgagccgcag tacgtcgagc tcggcttcga aagtggcctg ccaaagacgc tcgacggaca
     1921 agagctgccg cttgtagatc tcatctccga gcttggcgcc gtcgccggcg caaacggcgt
     1981 cggtcgcatc gacatgctag aggatcgcct ggtcgggata aagagccgcg agatctacga
     2041 agcccccgct gccctagcca tcatagccgc ccaccgcgaa ctcgaaaccc tgaccctcac
     2101 cagagacgtc cttcgcttca agacgaccgt cgaacagcgt tacgccgagc tcacctacga
     2161 gggcctgtgg ttcacccccc tcaaatccgc cctcgacgcc ttcgtcgcag agacccagaa
     2221 gaccgtcact ggcaccgtcc gcctcaagct ctacaaaggc aacagcacca tcgtgggccg
     2281 cagagcccct aacgccctct acagcgaaga gcttgccacc tacgatccca acagcacctt
     2341 cgacgaggcc gcagcagcag gcttcattgc cctctggggc ctctcagccc gccagtgggc
     2401 aagcgtcaac ggcggtgcgg aatacagcca gacatcctca caacattaaa cctgtggtcc
     2461 atactacagg ataataatag gccggtttgc agcacattta gtacagaagt acgtgaactc
     2521 gagcagcctg cagtataaac atatactcaa gttgagtatg aatctgtgct acagacttgc
     2581 gcttacgtgt tcggaatctc tgctacggtg ctcgggtttc gagttctgtg ggattcggcg
     2641 tctggagcgt cactactgct gtgaaagctc ctgctgtcct tgacacatcg actttctttg
     2701 atctatactt ggatcaagat ttcttgatgt aaggagttat tgtggcagaa gatctcaagc
     2761 agcgagtacg ggacaggtac gccggggcgg cgaggagcgt acttggggaa gctgaggatg
     2821 gcggttcgtg ttgcgggggt ggcgcgtctt gttgcggggt tgtggaatct ggggagcggg
     2881 cggcgttcga ggtagatatg gcctgtggga gctattccga ggctgagagg agtgagctgc
     2941 cacagctcgc cgccgatgct tcgctggggt gtgggaatcc cgtggcgctg gctacgctct
     3001 cgcccggtga ggtcgtgctc gacctcggca gtggcggcgg catagacgta ttgctctcgg
     3061 cgaggcgggt ggctcctgga ggcagagcct atggcctcga tatgaccgat gagatgctgg
     3121 agctggcacg gaggaaccag gccgaggctg gtgtcgagaa cgcggagttt tttaaaggtg
     3181 agatagagga cgtgcccctt cctgacgggc acgtcgacgt cgttatcagc aactgtgtga
     3241 tcaacctctc cacagagaag cagcgggtta ttggtgaatc cttcagggtt ctcaagccag
     3301 ggggccggtt cgccgtctcc gacgtggtgt tcttcggtga gaagaacaag ttgccccctg
     3361 ggatcgcgca gagcgtcgaa ctgtggtcag ggtgcgtctc gggagcgctg gagaaagacg
     3421 agtacgaagc gctgctctcg gaggccggct tcgaggaggt agtggtggag gtgacccaca
     3481 cgtatcctcc cgagcagata gcagggctct ccggcgaaga cgccgaggcc ctgcgtgagg
     3541 tccctgcggc cagtgccttt atcagggccc gcaaaccgga ggccttgtaa ccgtgacgca
     3601 gcggcaacta ccagtcctcc aggagagtag tgtcgcctcg ggaaccatag atatcggtgg
     3661 tgaggagcgc gccggacggc tcgtggccct tggccgggcg ctctcagacc cgatccgggt
     3721 acagatgtta gggatgctcg ccgaaggacg cgggtgctgt gagctacccg actgcggcgt
     3781 tccggcaagg gatcaggacg ccggcatctg cgtctgcgag ttcgagagat acttcgatgc
     3841 gggtcagtcg aaggtctcct accatcttgg caagctcaaa gaggcagggt tggtgcatga
     3901 ggagcggcgt ggcaaatgga gcttctactc cctcgaccgg acggccgcag gacaactcct
     3961 cgccgagacc gcggcccatc tggacggcag accaggcaat gatggctgac cggcgcgcaa
     4021 aagacagcac gcgggtcctg tttgtgtgca cccataactc ggcccgctcc cagatggccg
     4081 aagggttgtt gaggggcatg gccggggacc gtttcgaggt gttcagcgcc gggactgagg
     4141 cgacgcgtgt caggccggag gcggtctcgg tcatggctga gatcggggtg gatatctccg
     4201 cccaggagtc gaagacgctg gatcgctacc tcggagaaac cctggatctc gtcattaccg
     4261 tctgcgacga cgcgaacgag gcctgtccgg tcttcccggg agcaagagag cgcctgcact
     4321 ggtccttccc cgacccttcg aaagccgccg gtggccacga agagcgcctg cgggcgtttc
     4381 gatacgtgcg ggatgagatc cgagcccgta tcgaaggcga actggcgaaa ccaggggtgt
     4441 gatcccaacc tggctccgtc tgaagcgaca tcatcagcga aggacagact gctatcatcg
     4501 gcttcatgac ggcgccactg acacacgatc cttcggagac cgacctggcc atgatgcgcc
     4561 gcgccctgct cgccgctgag gagagcgccg aaaagggcga ggttcctgtc ggagccgtcg
     4621 tcgccagggg agatgagatt ctggccgtcg ccgcaaacga gcgtgaggct acaggggatc
     4681 ccaccgccca cgccga
//
LOCUS       DATMJA010000138         4678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_432116, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000138 DATMJA010000000
VERSION     DATMJA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432116"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VNT12_03225"
     CDS             <1..598
                     /locus_tag="VNT12_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HWI45158.1"
                     /translation="PRAEIEGEMGDSHVGLQARLMSQALRKLSGSLSRSGTTAIFINQ
                     LREKIGVMFGSPETTPGGRALKFYSSVRLDIRRIGALKVGSDAVGNQTRVKVVKNKVA
                     PPFKTVEFDVMYGEGISREGSLLDVGIEHDVVQKSGAWFAYGDERLGQGRENAKQFLK
                     ENHEVRERILDEIYEKLGLLEPVETADEVAEEPAGQVL"
     gene            694..1314
                     /locus_tag="VNT12_03230"
     CDS             694..1314
                     /locus_tag="VNT12_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HWI45159.1"
                     /translation="MPEITGVRERRNRTRVFVDGEFWAEIDAAVAIESGLREGAEFSQ
                     EELDRVRVAGERPIAMRRALNLLGYRARSEAEIRDRLQRYGFVGETVDAVVLRLEELG
                     YLDDAEFARLKVREKARRYGPRRVSLELKKSGVGETLAREVVAEEFAGRSEVGEARSA
                     AARRYNGRGSDAEARRVYGFLVRRGYSAEVCAQVAREYREPPEAEG"
     gene            1374..2918
                     /gene="rny"
                     /locus_tag="VNT12_03235"
     CDS             1374..2918
                     /gene="rny"
                     /locus_tag="VNT12_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWI45160.1"
                     /translation="MNVLLLVLGLVIGLAVGAALGYFVSKSRVDTRQAQARADARSIL
                     EDTEREVETKRREADLAAKESSLRIKDDAEAEVRARRAEIARVEERLDNRDTALDRRE
                     GELDERRRRLTETEEAMRRKEEQLREREADHLRMLEEISSLTREEAERRLFSELESEL
                     EGRLGRMVREKTLEAEERADAEARRLISTTMERLASDLTSESTVKAVSLPSDDMKGRV
                     IGREGRNIRAFEAATGVDVIIDDTPETVVISSFDPVRREVARVSMGRLVQDGRIHPGR
                     IEQIVAKARKDVEKEMKAAGRQALYDAKISGGMHNDLLRLLGALKYRTSYGQNVLAHS
                     IEVANLCGMMAQELGANVKISRRAALLHDVGKAIDHEVEGTHALIGGRFAKKSGESDD
                     VVRAISAHHHEIEMETVEDVLVATADAVSAARPGARRETTETYIERLRSLEDIALGHR
                     GVDKAYAIQAGREIRVMVQPSEVDDRIAAKLAYDISKQIETDLEYPGQIKVTVIRESR
                     VSEVAR"
     gene            2926..3849
                     /locus_tag="VNT12_03240"
     CDS             2926..3849
                     /locus_tag="VNT12_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI45161.1"
                     /translation="MEGPYRDLGAGFARLTGVEGARRGPSRITHVEDALLELARNARD
                     AGAANIYVASTLRDRRYRTLTVIDDGHGIPKSHKDLILEPGVTTRHLTPVTEPGDPVV
                     HGAGLSLYQIKGHSLETRVLSTSSPTSIQATFDTNVLPERALQSTTRPSKSNLRSTLK
                     RFTVITNAHSPRLHSYYGPPARILATLLHHRIIPKQRDSSGLREAAFGLGLDVSVRSV
                     QRILRGEVRPVAVVSGGEHDEGVGDRRAVGRRDGGPVLELGEEESARIADILRRAARA
                     GYMEVEELRFESRPGEISLRARVYEPEEQYE"
     gene            3842..>4678
                     /locus_tag="VNT12_03245"
     CDS             3842..>4678
                     /locus_tag="VNT12_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWI45162.1"
                     /translation="MSSTTKTTAPIPLSSKPQGAADPGAKTACIRTFGCQMNVHDSDR
                     IRRMIQDAGYAEVQRYEDADLVILNTCYVRENAVDRVKGHLGELNRLRREGRVKRVAL
                     TGCIGAAEESAALRQTYEIDLVLGTHNTYELAEFVGLPTMQETYTPELPGTEGGHNAF
                     VTIMTGCNYQCSYCIVPTVRGRMVCRPMESVIGEVENLVAGGTGYITLLGQTVDAWRH
                     EGKRFCDLLEAVSESAPRVWFTTSHPSNMEDRTLKAIGERDTIVKRLHLPVQSGSDRM
                     LKV"
BASE COUNT          974 a         1359 c         1604 g          741 t
ORIGIN      
        1 acccagggct gagatcgagg gagagatggg agactctcac gtgggacttc aggctaggct
       61 catgagccag gctttgcgca agctctcggg gtccctcagc cgctccggga ctacggccat
      121 tttcatcaac cagctgaggg agaagatagg ggtcatgttc ggctctccgg agacgacgcc
      181 gggagggagg gcgctgaagt tctattccag cgtgcgtctg gacatccggc gcatcggcgc
      241 gctgaaggtc ggcagcgacg ctgtgggcaa ccagaccagg gtcaaggtcg taaaaaacaa
      301 ggtggcaccg cctttcaaga cggtggagtt cgacgtgatg tacggagagg ggatctcccg
      361 tgagggaagc ctgctcgacg tgggcatcga gcacgatgtg gtccagaaga gcggggcctg
      421 gttcgcatac ggcgacgaac gcctggggca gggacgggag aacgccaagc agttcctcaa
      481 ggagaaccat gaggtccgcg agcgtatcct cgacgagatc tacgagaagc tcggtctact
      541 cgagcctgtg gagacggccg atgaggtagc cgaggagccc gctgggcagg tgctttaaag
      601 ctgcggtcga ggaggctcaa actcctcgac cgccttcagc atgtcagcac ttcagcctcg
      661 acacgggtcg acttctctac cctgggaggg tctatgcccg agataacggg tgttcgcgaa
      721 cgccgtaaca ggaccagagt ctttgtcgac ggggagttct gggcggagat cgacgccgcc
      781 gtggccatcg agagcggact tcgcgagggg gccgaattct cgcaggagga actcgaccgg
      841 gtgcgcgtcg cgggggagag gcccatcgcg atgaggcggg cgctcaatct gcttggatac
      901 agggccaggt cggaggccga gatccgcgac cggctgcagc gttacggatt cgtaggggag
      961 actgtagatg ccgtcgttct tcgcctcgag gagctcggct atctcgacga cgccgagttc
     1021 gcgcgtctaa aggtccgcga gaaggcgcgc aggtacgggc cgcggcgggt ctcgctcgag
     1081 ctcaagaaga gtggtgtggg ggagactctg gcacgggagg tagtcgcaga ggagttcgca
     1141 ggccgctccg aggtggggga ggctcgatcc gccgctgcgc ggcggtataa tgggcgtggg
     1201 tccgacgcag aagcgcgtag ggtctacggc tttctggttc gccggggtta ctcggcggag
     1261 gtatgtgccc aggttgcccg ggagtaccgg gagcctccag aggctgaggg atagtcgagc
     1321 tagcttgatg ccgggctgcc gcgtgcgcgg caggcccgct ggaaggacaa cctgtgaatg
     1381 ttttgttact ggtcctggga ctggtcatcg gcctcgccgt cggtgccgcc ctgggctact
     1441 tcgtctccaa gtcacgtgta gatacccgcc aggcccaggc cagggccgac gcaaggtcca
     1501 tactcgagga taccgaacgc gaggtcgaga ccaaacgccg cgaggctgac ctggcggcca
     1561 aagaatcgtc gctcagaatc aaagacgacg ccgaagccga agtacgtgcc aggcgggctg
     1621 agatcgcacg cgtcgaggag cgcctggata acagggatac agcccttgac aggcgtgagg
     1681 gcgagctcga cgaacgcagg cgccggctca cagagactga ggaggcgatg cggcgcaaag
     1741 aagagcaatt gagggagagg gaggctgacc acctcaggat gctcgaagag atctccagcc
     1801 tgacccgtga ggaggccgag aggcggctgt tctccgagct agagtcggaa ctcgagggcc
     1861 ggctcgggcg catggtgcgc gagaagaccc tggaggccga ggagcgggct gacgccgagg
     1921 cgcggcgtct gatctccact accatggaac gcctcgcctc ggacctgacc tcagagtcga
     1981 cggtcaaggc cgtgtctctg ccctctgacg acatgaaggg cagggtcata ggccgcgagg
     2041 gcaggaacat aagggccttc gaggccgcga cgggggtaga cgttatcatc gacgatacgc
     2101 cagagacggt ggtgatctcc agcttcgacc ctgtcaggcg cgaggtggcg cgggtctcaa
     2161 tgggacggct cgtgcaggac gggcgcatcc atccggggcg catcgagcag atcgtggcga
     2221 aggcccgcaa ggacgtggag aaggagatga aggccgcagg ccgccaggcg ctctacgacg
     2281 ccaagatctc aggcggcatg cacaacgacc tcctgcggct gctcggcgcg ctgaagtacc
     2341 ggacgtccta cggacagaac gtgctggccc actctatcga ggtcgccaac ctctgcggca
     2401 tgatggccca agagctcggt gccaacgtca agatctcgcg cagggcagcc cttctgcacg
     2461 atgtcggtaa ggccatagac cacgaggtcg agggtaccca cgccctgatc gggggccggt
     2521 tcgccaagaa gagcggggag tcagacgatg tggtgcgcgc cataagcgcc catcaccacg
     2581 agatcgagat ggagactgtc gaggacgtgc ttgtcgccac tgcagacgcg gtctcggcgg
     2641 cccgccccgg cgccaggcgc gagacgacgg agacctacat agagcgcctg cgctccctgg
     2701 aggacatcgc cctcggccac agaggggtag acaaagcgta cgcgatccag gccggacgtg
     2761 agatccgagt catggtccag ccctccgaag tggacgaccg gatcgccgcc aagctagcct
     2821 acgatatctc caagcagata gagaccgacc tcgagtatcc cggccagata aaggtgacgg
     2881 ttatccgcga gagccgggtc agcgaagtgg ccaggtaggc gagggatgga gggcccctac
     2941 agggacctcg gcgcaggctt cgcccgcctg accggcgtgg agggggcccg ccgcggtcct
     3001 tcccggatca ctcacgtcga agacgccctt ctggagctgg cacgcaatgc ccgcgatgca
     3061 ggggcagcca atatctacgt ggcctcgacc ttgagagacc ggcgctacag aaccctgacc
     3121 gtcatcgacg acggccacgg catcccaaag tcgcacaagg atttgatcct cgaacccggc
     3181 gttaccaccc gccatctgac ccccgtcacg gaaccaggcg acccggtcgt ccacggcgca
     3241 ggcctctccc tgtaccagat caaaggccac tccctagaga ccagggtact ctccacatcc
     3301 tcgcctacct cgatacaggc aaccttcgat accaatgtcc tcccggaacg tgccctccag
     3361 tcaaccaccc gaccctccaa aagcaacctc cgctccaccc tcaaacgctt taccgtaata
     3421 accaacgccc atagccccag acttcactcc tactacggac caccagcccg catcctcgcc
     3481 acgctcttac atcaccgcat aataccaaaa caacgagaca gttccggttt gcgggaggcg
     3541 gctttcgggc ttggacttga cgtttcggtg aggagcgtac agaggatatt gcgaggtgag
     3601 gtgcggcctg tggcggttgt ttcgggaggg gagcatgatg aaggggtcgg ggatcggagg
     3661 gctgttggga ggcgagatgg ggggcctgtg ctggagcttg gggaggagga aagtgcgcgc
     3721 attgcggata tactcaggcg ggcggcgcgg gccgggtaca tggaggtcga agagttgagg
     3781 tttgagagcc gtccggggga gatttccctc agagcccgag tctacgagcc ggaggaacag
     3841 tatgagtagc acgacaaaga cgacggcgcc cataccgctc tccagcaagc ctcaaggggc
     3901 ggccgaccct ggagccaaga ccgcctgcat caggaccttt gggtgccaga tgaacgtgca
     3961 cgactcagat cgtataaggc ggatgatcca ggacgcaggc tacgcggaag tccagcgcta
     4021 cgaggatgcg gatctcgtaa tactgaatac ctgctacgtc cgcgaaaacg ccgtggaccg
     4081 cgttaaaggg cacctcggag agctaaaccg cctccgccgc gagggacggg tgaagagggt
     4141 ggccctcacg ggctgcatcg gcgcggccga ggagtcggca gccctaaggc agacctacga
     4201 aatagacctc gtactcggca cccataatac ttacgagctg gcagagttcg tggggctacc
     4261 gacgatgcag gagacgtaca cgcccgaact gccgggcacc gagggtgggc acaacgcctt
     4321 cgtcacgata atgaccggat gcaactacca gtgcagttac tgcatagtcc ccaccgtgag
     4381 ggggcgtatg gtctgccgcc cgatggagag tgtgatcggg gaggtagaga acctcgtcgc
     4441 tggcggcaca ggctacatta cgcttctggg ccagacggtg gacgcgtgga gacacgaggg
     4501 aaagaggttc tgcgatctcc tggaggctgt ctcggagagt gcgccgcggg tatggtttac
     4561 gacgagccac ccctcaaaca tggaggaccg cacgctaaag gctatcgggg agcgggatac
     4621 cattgtgaag cggcttcatt tgcccgttca gtcggggtcg gacaggatgc tgaaggtc
//
LOCUS       DATMJA010000139         2421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_373093, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000139 DATMJA010000000
VERSION     DATMJA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373093"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /gene="pyrH"
                     /locus_tag="VNT12_03250"
     CDS             <1..207
                     /gene="pyrH"
                     /locus_tag="VNT12_03250"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564379.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWI45163.1"
                     /translation="RHSDAVRLPRLNYMDLLSNDLSVMDHTAATLCGSENLPIVVFDI
                     LKPGNLRRILQGEQVGSLVWGAAK"
     gene            218..778
                     /gene="frr"
                     /locus_tag="VNT12_03255"
     CDS             218..778
                     /gene="frr"
                     /locus_tag="VNT12_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWI45164.1"
                     /translation="MSDIIDLSAAQRRMKGAVESVRSNFATIRTGRANPSLLDRIEVE
                     VYGSRMPLRSVANIGAPEARLLTVTPFDPNSLKDIERAIRDADIGLNPQNDGKILRLP
                     IPELTEERRRDLIRMARSMAEEGRVSVRNVRRDEMRDLHELRKEGEISQDDEHRAEDE
                     LQKLTDGYVKRVEAALADKEAELSEV"
     gene            860..1528
                     /gene="uppS"
                     /locus_tag="VNT12_03260"
     CDS             860..1528
                     /gene="uppS"
                     /locus_tag="VNT12_03260"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWI45165.1"
                     /translation="MAIIMDGNGRWAKSRWLPRAAGHRAGVSVLTPLLETAGRAGVKT
                     LTLYAFSTENWARPEREIKTLMKLFMDTAREKVPELNERGARLRFLGRREHLPDSVLE
                     AMEEAEDLTSGNDSLDVYIALDYGGRAEIVDATRRIVADGLSPEDVDEMTFAGYLYAP
                     DVPEVDLVIRTSGELRISNFLLWQIAYAEFYVTDTLWPDFTPKEFLRALESFGSRSRR
                     RGGV"
     gene            1536..2306
                     /locus_tag="VNT12_03265"
     CDS             1536..2306
                     /locus_tag="VNT12_03265"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWI45166.1"
                     /translation="MLWWRAATALVLAPIALASIAIGKWAMIALLLVVIAGAAYELSR
                     ALEPLPFVAALGAGALPLLLSIPYHETGVLAGAVVGLPWALIWLAARPETRTLRAVLA
                     VLLMSLWVGVPLAHLGLFPHSGYGVVLILIAVVGPWISDSGAYFAGRFFGRNLLFPTL
                     SPNKTVEGSVGGLLLTVLIVAPVALQFLDFTIAKALVLGAGVSVSSQCGDLFESSLKR
                     ILDVKDLGNFLPGHGGILDRVDSLIFTAPTVYYISLLV"
     gene            2323..>2421
                     /locus_tag="VNT12_03270"
     CDS             2323..>2421
                     /locus_tag="VNT12_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45167.1"
                     /translation="MRRRLTILGSTGSIGRQALDVVRRYPERFEIVG"
BASE COUNT          448 a          780 c          746 g          447 t
ORIGIN      
        1 cggcattccg acgccgtgcg cctgccgcgg ctcaactaca tggacttgct ctccaacgac
       61 ctatccgtta tggaccatac ggcggccacg ctgtgcggca gcgagaacct gcccatagtc
      121 gttttcgata tacttaaacc aggtaacctg cgccgtatct tgcagggaga acaggttggg
      181 tcgctggtgt ggggcgccgc gaaatgagga ggatgagatg tctgacatca tagatctctc
      241 ggcggcccag cgcaggatga aaggggcagt cgagtccgtt cggagcaatt tcgcaactat
      301 acgcacgggc agggccaacc cttcactgtt ggacaggatc gaggtcgagg tctacgggtc
      361 taggatgccc ctgagaagcg tcgccaacat cggcgcgcca gaggcacggc tcctcaccgt
      421 gacccctttc gacccgaact ccctgaagga catcgaacgt gccataaggg acgcagatat
      481 cggcctcaac ccgcagaacg acggcaagat actgaggctt cccatccctg aactgacgga
      541 ggagcgccgc cgggacctaa tcaggatggc gaggagcatg gccgaggagg gaagggtctc
      601 cgtcagaaac gtgcgccgcg acgagatgag ggacctgcac gagttgcgca aggaaggtga
      661 gatctcccag gacgacgagc acagggcaga ggacgaacta cagaagctca ccgacggcta
      721 cgtgaagagg gtcgaggcag cccttgccga caaggaagcc gagctctcgg aggtctagct
      781 gctggaaccc gtcgagaaac gaccccccac agactccccc gagcagcgcg tcctcgaccc
      841 cgaacggagc ccgcgtcacg tggccattat catggatggc aacgggcgct gggcgaagag
      901 tcgctggctt ccgcgtgcag ccggccacag ggcaggtgtc tcggtgctca cgccactgct
      961 ggagaccgcc gggcgcgccg gggtgaagac gctgaccctc tatgccttct cgaccgagaa
     1021 ctgggcgcgt ccggagcgag agataaagac gctcatgaag ctcttcatgg acacggcccg
     1081 cgagaaggtg cccgagttga acgagcgtgg ggccaggctc cgttttctgg gcaggaggga
     1141 acacctgccc gactctgtgc tggaagcaat ggaggaggct gaggacctta cctcgggcaa
     1201 cgacagcctc gacgtctaca tcgcgctcga ctacggagga cgcgccgaga tcgtggacgc
     1261 cacccgccgg atagtcgcag atggtctctc tcctgaggac gtcgacgaga tgaccttcgc
     1321 cggttacctc tacgcccccg acgtacctga ggtcgacctg gtgatccgga ccagcggcga
     1381 gctgcgcatc tctaacttct tgctctggca gatagcctac gcagagttct acgtgacgga
     1441 tacgctgtgg cccgacttca ctcccaagga gttcttgcgg gccctcgagt cattcgggtc
     1501 ccggtccagg cgccggggcg gcgtctaggg tccctgtgct ctggtggagg gcagcaaccg
     1561 cccttgtact cgcgccgatc gcgctggcca gcatagccat cggcaaatgg gccatgatcg
     1621 ctttactact cgtagtcata gcgggggccg cctacgagct ctcgcgggcg ctcgagccgc
     1681 tgcctttcgt tgcggccctt ggcgccggcg cgctcccact gctactctcc atcccttacc
     1741 acgaaaccgg tgtactggca ggtgccgtag ttggcctgcc ctgggccctg atctggctcg
     1801 ccgccaggcc cgagacgagg acgctgcgtg ctgtgctcgc cgtactcctc atgtcgctct
     1861 gggtcggcgt cccgctggcg cacctgggac tgtttcccca ttccggctac ggcgtcgtcc
     1921 tcatcctcat agccgtggtg gggccctgga tctccgactc cggcgcgtac ttcgccgggc
     1981 gctttttcgg ccgtaacctg ctgttcccga ccctgagccc taacaagact gtggagggca
     2041 gcgtcggcgg cctgctattg acggtcctga tcgtcgcccc cgtcgctctc cagttcctcg
     2101 actttaccat cgccaaggcc ctcgttttgg gcgccggggt ctctgtctcg agccagtgcg
     2161 gagacctctt cgaatcttcc ctaaagcgta tcctcgacgt gaaggatctc ggtaacttcc
     2221 tccctggcca cggaggcatc ctcgaccggg tggacagcct gatcttcacg gccccgacgg
     2281 tatactatat ctccctactc gtatgagatt ggacccacca acatgagacg ccgcctcacg
     2341 atcctcggct cgacgggcag tatcggcaga caagccctcg atgtcgtccg caggtatcct
     2401 gaacgcttcg aaatcgtcgg t
//
LOCUS       DATMJA010000140         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_847129, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000140 DATMJA010000000
VERSION     DATMJA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847129"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VNT12_03275"
     CDS             complement(<1..516)
                     /locus_tag="VNT12_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWI45168.1"
                     /translation="MKPRITRRRFLQAAAGGAAWVALVGALGCDPVRRARATASFAPA
                     GQAWSFRSRPDLHPPVIEVTRRARDTAPGRIFVAPKNGPDEAGPGQDGCMILDNDGQP
                     VWLRLLQIEDVDVMNFKVQTYKGETVLTWWEGLHTGYGQGEYVIFDHSYREIKRFGAG
                     NGYEGDHHEFLI"
     gene            complement(589..>2447)
                     /locus_tag="VNT12_03280"
     CDS             complement(589..>2447)
                     /locus_tag="VNT12_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWI45169.1"
                     /translation="PQWLFKITDYAEELLDFSKIDWPERVETLQRNWIGRSEGAGIRF
                     EIEGYGPVEVFTTRPDTLFGATFFVVSPEHPAVEKITTPEQAVEVRAYVERAARMSEI
                     DRTDVTREKSGVFTGACAINPANGEEIPVYIADYVLMGYGTGAIMAVPAHDERDFEFA
                     RKYGIEIRTVIAPPDWDGGDLAEAYAGEGTMVNSGRFDGTPSTEGKEKVTAWLEERGA
                     GEATVTYRLHDWLISRQRYWGTPIPIIFCDECGIVPVPEEDLPVLLPEEAEFMPTGES
                     PLKLDPDFYNTECPRCGGPATRETDTMDTFVDSSWYWYRYLSPHYDEGPFDPERGEKW
                     LPVDQYTGGIEHAILHLLYARFFTKVMRDLGLVDFDEPMTSLRNQGNILGPDGQKMSK
                     SRGNVVNPQEYVDRFGSDALRGFLMFIGPWNEGGPWDGKGIEGVSRWLRRALSLVTRD
                     DSSGGEADPKELERRTNRLIKKITDDLEAFRFNTSIAALMEHTNYLLAVRGRVGDGEW
                     HEALRTFVTVLSPFAPHHAEEMWAAMGEEYSVHEQAWPGYEETLIQAKEITLIVQVNG
                     KLRDRIEAPADISEVAARELALSSARVRPHLEGKELRKSIYVPGRLVNLVVG"
BASE COUNT          386 a          804 c          764 g          493 t
ORIGIN      
        1 gatgaggaac tcgtgatggt ccccctcgta gccgttgccg gccccgaacc tctttatctc
       61 cctgtaggag tggtcgaaga tcacgtactc gccctggccg tatcctgtat ggagtccctc
      121 ccaccaggtg agcaccgtct cccctttgta cgtctggacc ttgaagttca tcacatccac
      181 atcctcgatc tgcaagaggc gcagccacac gggctgtccg tcgttgtcga ggatcatgca
      241 gccgtcctgc cccggaccgg cctcatcggg tccgtttttc ggggcgacga agatgcggcc
      301 gggagcagtg tcacgcgccc gcctggtcac ctcgatgacc ggcgggtgga gatccggacg
      361 cgagcggaag ctccacgcct gtcctgccgg ggcgaatgag gcggtcgccc gggcgcggcg
      421 gaccggatcg cagcccaggg cgccgacgag cgcgacccac gccgcaccgc cggccgctgc
      481 ttgcaggaac ctccttcgcg taatcctggg tttcaagctg ccactgtctg gctcttgccc
      541 tggctacggc tccgcctggc gtatctcgta acgtggcgta tgatcacgct atcctacgac
      601 gaggttgacg agcctgccag gcacgtagat gctcttgcgg agctctttgc cttcgaggtg
      661 gggacggacc ctggcgctcg acagtgccag ctctctggct gccacttcgg agatatctgc
      721 tggggcttcg atgcggtcgc gcagcttgcc gttgacctgg actatgagcg tgatctcctt
      781 agcctggatc agggtttcct catagcctgg ccacgcctgc tcgtgcaccg agtactcctc
      841 ccccatcgcg gcccacatct cctcggcgtg gtgcggcgcg aacgggctga ggaccgtgac
      901 gaaggtccgc aaggcttcat gccactcgcc gtccccgacg cggcctctga ccgcgagcag
      961 gtagttggtg tgctccatga gcgcggcgat ggacgtgttg aagcggaagg cttcgaggtc
     1021 gtctgtgatc ttctttatca ggcggttcgt ccggcgctcc agctctttcg gatctgcctc
     1081 gccgcccgag gagtcatccc ttgtcacgag cgagagggcg cgtctgagcc agcgcgagac
     1141 accctctatg cccttgccat cccagggacc gccttcgttc cacggaccga tgaacatcag
     1201 gaagccccgg agtgcgtcgg agccgaagcg gtctacgtac tcctgcgggt tgaccacgtt
     1261 gccccggctc ttgctcatct tctgaccgtc gggcccgagt atgttcccct ggttgcgaag
     1321 gctggtcata ggctcgtcga agtcaaccag cccaagatcg cgcatgacct tggtgaagaa
     1381 gcgggcgtag agcaggtgca aaatggcgtg ctctatgccg ccggtgtact ggtcgacggg
     1441 gagccacttc tctccccgtt cgggatcgaa cggcccctca tcgtagtgcg gcgagaggta
     1501 acggtaccag taccacgagg agtccacgaa agtgtccatc gtgtccgtct cgcgtgtggc
     1561 agggccgccg caacggggac actcggtatt gtagaagtca gggtcgagct tgaggggcga
     1621 ttctcccgtc ggcatgaact cggcttcctc ggggagcagc acagggaggt cctcttcagg
     1681 gacggggacg atgccgcact cgtcgcagaa gattatgggg atcggggttc cccagtagcg
     1741 ctggcggctg atcaaccagt cgtgaagccg gtaggtgacc gtagcctctc cggcgccgcg
     1801 ctcctcgagc caggccgtca ccttctcctt gccctcggtg ctcggggtgc cgtcgaacct
     1861 acccgagttg accatcgtgc cctcgccggc gtaggcctct gcgagatctc cgccgtccca
     1921 atccggcggc gctatcacgg ttctgatctc gatgccgtac ttcctcgcga actcgaagtc
     1981 tcgctcgtcg tgggccggga cggccatgat cgcgccagta ccgtagccca tcaggacgta
     2041 gtcggcgatg tagacgggga tctcttcccc gttggccgga tttatcgcgc aagcccccgt
     2101 aaagacaccc gacttctccc tcgtcacgtc ggtgcggtcg atctcgctca tcctggcggc
     2161 cctctcgacg taggcgcgca cttctacggc ctgctccggg gtcgttatct tctcgaccgc
     2221 cggatgctcc gggctcacaa cgaagaacgt ggcgccgaag agcgtgtcgg ggcgggtggt
     2281 gaagacctcg actgggccgt agccttcgat ctcgaacctg atcccggcac cctcggagcg
     2341 cccgatccag ttccgctgca gcgtctcgac ccgttcgggc cagtctatct tcgagaagtc
     2401 cagcaactcc tcggcgtagt cggtgatctt gaagagccac tgcggca
//
LOCUS       DATMJA010000141         5510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_491907, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000141 DATMJA010000000
VERSION     DATMJA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491907"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /gene="acpP"
                     /locus_tag="VNT12_03285"
     CDS             <1..199
                     /gene="acpP"
                     /locus_tag="VNT12_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWI45170.1"
                     /translation="RLGVDEGDVTSDASFREDLEADSLDLVELIMELEEQFGMEIPDE
                     EAEKITTVEEAVDYVMEHQAA"
     gene            196..1452
                     /gene="fabF"
                     /locus_tag="VNT12_03290"
     CDS             196..1452
                     /gene="fabF"
                     /locus_tag="VNT12_03290"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWI45171.1"
                     /translation="MTDPDDNGRGRALVTGTGAVTPLGTGVENFWDAALHGKSGIGEI
                     TLFDPSPYPSRIAGECWDFDPTDFIKKKQARRMDRFAQLGIAAGIQAAESAGIVDLIR
                     EGPERVAIVMGSGIGGLSTWEREYRVMNERGPGRVSPFLITMMVPNMAAGQLAIALGA
                     TGPSQCPVLACATGGEAIAEGLELIRRGDADVVIAGSSEAPITPLSMAGFCAIRAVSR
                     RNDDPARASRPFDEGRDGFVMSEGAGALVLESEEHAERRGAEPISAVAGYGRTTDAYH
                     VTAPDEDGRGVERAMVHAFQDSGLDAEEVGHVNAHATATPTGDGPETKAIARQVPHAL
                     VSGTKSMTGHSFGAVGATEGIMCALAIKHKVVPPTINLENLAEDCEELNYVGSEPVEA
                     PDLKAAISNSMGFGGHNVVIAFTSVE"
     gene            1461..2120
                     /locus_tag="VNT12_03295"
     CDS             1461..2120
                     /locus_tag="VNT12_03295"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HWI45172.1"
                     /translation="MSIRDLIAILPEPLRQRSLTHPSVADPYESNGRLEFLGDSVLNS
                     RVAELVFHGYPELLEGDLTRIRAHLVRKSFLAMVGRSEGIEEIIESSVINDSVIADTV
                     EAIIGGAYLIDRDLVLRTLDEIFNPAEVDTDELRDWKTLLQETLQADGLRPTYRVISK
                     QGPAHRPSFTSRVSVDGREVATGEGSSIKESEQTAARNALEILGIRPVPRSTVEVHVG
                     A"
     gene            2476..>5510
                     /locus_tag="VNT12_03300"
     CDS             2476..>5510
                     /locus_tag="VNT12_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI45173.1"
                     /translation="MLSAIYIKGFKTFARPVRMPLEGGITSIVGPNGSGKSNITDAVL
                     FAIGEQSPGVLRAGAMSELIFAGSETLSGANAAEVTLVLDNEDGRIPLPYGEVSITRR
                     ISRSGGTEYRINGSRARLADVRAVAGEAGLGRHSILRQGAVDAIVAGGAAACRLALEE
                     AAGLGVYRRRRVSASRRLERADVQLERSRQLEAELAGQLRRIEREAAAARAYREIESR
                     YRELTLAHLYRVATKGLDGLRGRLEEARKQAAELASRERDVLKEESGIESRLQQLEDG
                     LGKTEQRLEALEDGAEELRVEALRSDRTLLRLEAGRGRGGETRLAVSRLEDELRRVLQ
                     TLASLEERARDLEAESLERRAESDRLQKVVARSSEKSAYAESERDRLARELEGLRTRL
                     AGLGSHSERRVMSEESLSHLAGVISDLGEAVGHATKENLRGLRDQVDGMLLGVDEHAE
                     EVSRRRGALAAAVGRAESRIRALGETAPDGASTRLYEVIRARPGYEVAVEAALGEYGA
                     GVLAENLDEGMKLVSDAEPVAVRLDARAMESNGGLPGKPLIECVEVLDARYDEAVRRL
                     LTGIFVVERPDDGANANGYVAVTRDGFRLTRTGVSLRPGPGNFARETRLAAWLEHLEA
                     LKDGPGGTLYDLRDTVSGVSDRLENLAGTVERMTILADRTARAQALVSRQARRRKTAG
                     ENSRRSFLERERTLSILADDASRTERALAEAETDADRVREELAAATAAAEATGAAAAD
                     SDRRLSELRATLATGESRREAISRRLERSANALEVGTGNVEAFASRAARAATKLVTTV
                     RRRRSALRRLRGEAADAHRRVSAERTALSRKTVDLAGKLATAHAQAERLAEEVSRAET
                     AAAEAGEEIQAEWGATLDAARQEAEKHEESTDGERQRLARKLKRFGDVNLLALSQEEG
                     LRERHQFISAQRADAEAAANELDRIIHSVDREIESRFAETFGRVRDAFGEMVPRMLEG
                     SSGVLDLSEEGVEIGLRLGRKGWRPLRVLSGGERALLALS"
BASE COUNT         1093 a         1638 c         1912 g          867 t
ORIGIN      
        1 caggctggga gtagatgaag gcgacgtcac atctgacgct tccttccgcg aggacctgga
       61 ggctgactcc ctggatctgg tggagctcat catggagctc gaggagcagt tcgggatgga
      121 gatccccgat gaggaggccg agaagatcac cactgtcgag gaggccgtcg actacgtcat
      181 ggagcatcaa gccgcgtgac cgaccctgat gataatggga gggggcgtgc gctcgtaaca
      241 ggaaccgggg ctgtgacgcc ccttggaaca ggggtcgaga acttctggga tgcggccctg
      301 cacggtaaga gcggaatagg ggagataacg ctcttcgacc cctcccccta tccttcgcgg
      361 atcgccggtg aatgctggga tttcgatcct acggacttca taaagaagaa gcaggcccgc
      421 aggatggacc gcttcgccca gcttggtata gccgcgggca tacaggccgc cgagagcgca
      481 gggatagtgg acctgatccg cgaggggccc gaacgcgtgg cgatcgtgat gggcagtggt
      541 atcggggggc tctcgacctg ggagcgcgag tacagggtca tgaacgagcg tgggcctgga
      601 agggtgagcc cgttcctgat cacgatgatg gtcccgaaca tggcggccgg gcaacttgct
      661 atcgcgctcg gcgccacggg gccgagccag tgtcctgttc tcgcctgcgc cacgggaggg
      721 gaggccatcg cggagggtct ggagctgatc cggcgcgggg acgctgacgt ggtgatagcc
      781 ggctcctcgg aagcccccat aacgcccctc tctatggctg gattttgcgc gataagggcc
      841 gtgagccgcc gtaacgacga ccccgcccgg gccagccgtc ccttcgacga gggccgcgat
      901 ggcttcgtga tgagcgaggg cgccggagca ctggtcctgg agagtgagga gcacgcggag
      961 cgccgcggcg cagaaccgat ctccgccgtc gccggctacg gccgcaccac cgacgcctac
     1021 cacgtaactg cgcccgacga ggacggccgc ggcgtcgaac gggcgatggt ccacgcgttc
     1081 caggattccg gcctcgacgc cgaggaggtc ggacacgtca acgcccacgc cactgccacc
     1141 ccgaccggag acgggccaga gacgaaggcg atagcaaggc aagtgccgca cgccctggtc
     1201 tctggcacga agtcgatgac ggggcactcc ttcggggcgg tcggcgccac agaaggcatc
     1261 atgtgcgccc tcgccataaa gcacaaagtc gtcccaccga cgatcaacct cgagaacctg
     1321 gcggaagact gcgaagagct gaactacgtg ggaagcgagc cggtcgaggc ccccgacctc
     1381 aaagccgcca tctcgaattc gatgggattc ggcgggcata atgtcgtgat cgcgtttaca
     1441 agtgtagaat agagaacgag atgtctataa gagacctcat agcgatcctt ccagaacctt
     1501 tgaggcagcg ctcgttgacc catccgagcg tcgcggaccc ctacgagtcg aacgggaggc
     1561 tggagtttct cggggactcc gttttgaaca gccgcgtggc cgagcttgtc ttccacggct
     1621 accccgagct actggagggg gatcttacgc gcatcagggc tcatctggtg cggaagtcct
     1681 ttctggcaat ggtaggccgg agcgaaggca tagaggagat catcgagagc tccgttataa
     1741 acgactcggt catcgctgat actgtggagg ccattatcgg gggggcctac cttatagaca
     1801 gggaccttgt gcttcggacg ctcgatgaga tattcaaccc ggcagaggtc gacaccgacg
     1861 agctacgtga ctggaagacg ctcctgcagg agactttgca ggccgacggg ctcaggccga
     1921 cgtaccgggt catctccaag caggggccgg cccacagacc aagtttcacc tccagggtct
     1981 ccgtggacgg acgcgaagtc gccaccggtg aaggcagctc cataaaagag agcgagcaga
     2041 ccgcagccag aaacgcgctg gagatcctcg gcatccgccc cgtgccacgc tcgaccgtcg
     2101 aggtacacgt aggcgcctga accaggatct cgtcgtgacg gttgagggcc gagcgcatcg
     2161 ctgtagcgtc acgctcccac agtccggttg agccctcgat gtcagtgaac agtaaagtta
     2221 ttgtgccgct gggcagggcg accagtcgct actacgcatc ctccttctcc acaaacggtc
     2281 accatagcat cggcactgct cacgcggaag ccgaaagccg ccgaacgctg ccttagcctc
     2341 ggccgcgggc cgccctgggt caaagaagct accctgcctc cagggcactc ctggcgtccc
     2401 tgcgcctgct gacggctcat ttgctgcact tttccgcgtg ctttcaggac aaaagttgcg
     2461 taaaatacgt gttcagtgct ctcggcgatc tatatcaagg gcttcaagac cttcgcccgg
     2521 ccggtcagga tgccgctcga agggggcatc acttcgatcg tcggtcccaa cggttcgggg
     2581 aagagcaaca tcaccgacgc cgtgctcttc gcgatcgggg agcagagccc tggggtattg
     2641 cgggctgggg ctatgagcga gttgatcttc gccggctcgg agacgctctc gggtgcgaac
     2701 gcggccgaag taaccctcgt actcgacaac gaagacggcc ggatcccgct cccttacggg
     2761 gaggtatcga tcacgcgccg gatctcccgc agtgggggga cggagtacag gatcaacggc
     2821 tcccgtgccc gtcttgcgga tgtacgcgcc gtcgctggcg aggcaggact gggacgccac
     2881 agcatactgc gccagggcgc cgtagacgca atagtcgctg gtggggcggc cgcgtgccga
     2941 ctcgcgctgg aagaggctgc gggacttggc gtctatagga ggcgccgtgt ctcggcgagc
     3001 cgccgtctgg agagggccga cgtccaactg gaacgtagcc gccagctgga ggctgagctt
     3061 gcaggccagt tgcgcaggat agaaagggag gccgctgccg cccgcgcata cagggagatc
     3121 gaatcccgct accgcgagct cactctggcg cacctctacc gggtcgcaac caaggggctg
     3181 gacggactca ggggaaggct cgaggaggcc aggaagcagg ctgcagagct ggcctcacgc
     3241 gagcgggacg ttcttaaaga agagtcaggg atcgaaagcc gcctgcagca actcgaagat
     3301 gggctaggaa agaccgaaca gcgactcgaa gccctggagg acggcgcaga ggaactgcgg
     3361 gtggaggccc tgcgctcgga caggactctc ttgcgtctgg aggcgggccg gggacgcggg
     3421 ggggagacta gactcgccgt ctcccgcctg gaagatgagt tacgccgggt gttgcagacg
     3481 ctggcttctc tcgaagagcg agccagagat ctagaggctg agtctttaga gaggcgggca
     3541 gagtcagacc ggttacagaa ggtcgttgca agatccagcg agaagagcgc gtacgctgaa
     3601 agtgagaggg acaggctggc cagggagctg gagggactac gcacgcgcct cgccgggctt
     3661 ggatcccaca gtgagcgacg cgtgatgtcc gaagagagcc tctcgcacct tgcgggtgtg
     3721 atctcggacc ttggagaagc ggtaggccac gcgacgaagg agaatcttcg ggggctgcgc
     3781 gaccaggtag acggcatgct tctaggggtg gatgagcacg ccgaggaggt cagtcgcagg
     3841 cgcggggccc tcgccgctgc tgttgggagg gctgaatcca ggatacgcgc tttaggggag
     3901 acggccccgg acggcgcgag cacgcggctc tacgaggtga tccgggcccg ccctggctac
     3961 gaggtcgccg tcgaggcggc ccttggagag tacggtgccg gggtgcttgc ggagaacctc
     4021 gacgagggga tgaagcttgt ttcggacgcc gagcctgtgg ccgtccgcct cgacgcgagg
     4081 gccatggaat ccaacggggg cttacctgga aaacccctga tcgaatgcgt agaagttctg
     4141 gacgcgcgct acgatgaagc ggtacggcgt ctcttgaccg gcatctttgt cgtcgagcgt
     4201 cctgatgacg gggcgaacgc caacgggtac gtggctgtta cgagggacgg ttttcgcctc
     4261 acgaggaccg gcgtgagcct gcgtcccggc cctggcaact tcgcacgcga aacgcggctc
     4321 gccgcctggt tggaacacct cgaagctctg aaggacgggc ccggcggaac cctctacgac
     4381 ctgagggata ccgtctcggg cgtctcggat cggctggaga acctcgctgg aaccgtagag
     4441 aggatgacga ttctcgccga ccggacggcg cgcgcccaag ccctggtctc acgccaagca
     4501 agacgccgca agaccgcagg ggagaactcc aggagatcct ttctggagcg ggagaggact
     4561 ctctctatcc tggccgacga cgcttcccgg acggagagag cgcttgcgga agccgaaacg
     4621 gatgctgacc gcgtgaggga ggaactcgcc gcagctacgg ccgccgccga ggcgaccggc
     4681 gcggccgctg ccgactcgga cagaagactg tcggagttgc gcgctaccct ggccactggc
     4741 gaaagtcgca gggaggctat ctcgcgcagg ctagagagat cagcgaacgc tctagaggta
     4801 ggaacaggca acgtagaagc gttcgcctcc agggcggcga gggcggccac aaagctcgtc
     4861 acaaccgtcc gccggcgtag aagtgctttg cggcgtctcc gcggagaggc ggcagatgct
     4921 caccgccggg tctccgccga gcgcacggcg ctctcgcgca agaccgtgga tctcgcaggc
     4981 aagctcgcca ccgcccacgc gcaagccgaa cgcctcgccg aagaggtgtc cagagccgaa
     5041 accgcggcgg ccgaggccgg cgaggagata caggcagagt ggggcgccac cctggacgct
     5101 gcccgccagg aggccgagaa gcacgaggag agcaccgacg gagaacgcca aaggctcgcc
     5161 cgcaagctca agcgctttgg ggacgtaaac ctcctcgctc tctcccagga agagggactg
     5221 cgcgaacgcc accagtttat ctcggcccag agggccgacg ctgaggccgc agctaacgaa
     5281 ctcgaccgca taatacattc cgtggacagg gagatagagt cgcgtttcgc ggagaccttc
     5341 gggagggtaa gggatgcatt cggggagatg gtgccacgga tgctcgaggg atcttctggg
     5401 gtcctggatc tctctgagga gggggtggag atcgggttgc ggctggggag gaagggttgg
     5461 agacccttgc gcgtcctatc agggggcgaa cgggccctac tcgcgctgtc
//
LOCUS       DATMJA010000142         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_551070, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000142 DATMJA010000000
VERSION     DATMJA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551070"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VNT12_03305"
     CDS             complement(<1..494)
                     /locus_tag="VNT12_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI45174.1"
                     /translation="MNSIEVSGLTKKYGRVEALGGADLSVGRGEVFGLVGPNGAGKTT
                     LIKGLVGALRPSGGTARVMGLDPLKDRAELRQRIGYMPQGPALYEDLTARGNVGFFGA
                     AHRTPDLKKKVDEVMHFTDLLGRADDPVHTFSGGMKRRVSLACALVHRPEVIFLDEPT
                     AAVDP"
     gene            complement(491..1105)
                     /locus_tag="VNT12_03310"
     CDS             complement(491..1105)
                     /locus_tag="VNT12_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI45175.1"
                     /translation="MAEETDRRAQILEAAFEEFSAKGFKGATIKSIARTASLQSPALI
                     YWYFPDKEALFREVLESRIPVLRAVRDPARLLNLPPEEVLPNIARRYLSTFDNRATQR
                     MARLLVGEAMRRPEVSEVFGNAVIKRVLGFLKSYMARQVELGRLTPHDVRASARAFIG
                     MLLPQAGGKMFFAAIREDGLTDEEHIEAVVGIFLRGLEGKEDKR"
     gene            1237..1467
                     /locus_tag="VNT12_03315"
     CDS             1237..1467
                     /locus_tag="VNT12_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45176.1"
                     /translation="MRNPILAAILSLIVAGLGQIYNGQIGKGVIFIVIQLINGALTAV
                     LIGWILLPIVGLWAMIDAYMTAKRNNERYSFR"
     gene            1478..1669
                     /locus_tag="VNT12_03320"
     CDS             1478..1669
                     /locus_tag="VNT12_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45177.1"
                     /translation="MDRRDLLTLESAVTAIEEAASAVAREVERDRLRETSLARLSTVE
                     AELHRSQLALEKIIQEETA"
     gene            complement(1780..>2449)
                     /locus_tag="VNT12_03325"
     CDS             complement(1780..>2449)
                     /locus_tag="VNT12_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWI45178.1"
                     /translation="LPQIRGPEPEVSAPEGSIVGDPDVQAASASTLRVRGIACGYGVE
                     GSGWVAGRNLVVTNAHVVAGETKTHVQVVATERRLPAKVVVFDEKNDIAILRVNGLGL
                     TPLHLAAPKPGKDAAVIGFPENGPLDIEPARTGTTQRVISSDAYDSGPVERVVTSFRV
                     YVRPGNSGGPAVNEYGRVVSTIFASRTGSDNSGYGIPSRIVHRHLRVAADRTTPVSTE
                     GCAN"
BASE COUNT          453 a          819 c          704 g          473 t
ORIGIN      
        1 gggtccacgg ccgccgtcgg ctcgtcgagg aagatcacct cgggcctgtg gacgagggcg
       61 caggccagcg agacccgccg cttcatccct ccggagaagg tgtgcacagg gtcgtcagcc
      121 ctccctaaga ggtctgtaaa atgcatgacc tcatctacct tcttcttgag atcaggcgtg
      181 cggtgtgcag cgccgaagaa ccccacgttg cccctggcag tgaggtcttc gtacagtgcc
      241 ggaccctggg gcatgtaccc tatcctctgc ctcaactcgg ccctgtcctt gagcgggtcc
      301 aaccccatca cccgcgccgt gccgccggag ggccgcagcg ctccgacgag acccttgatc
      361 agggtggtct tccctgctcc gttcggccct accaaaccga acacctcgcc cctgccgacc
      421 gagaggtccg cgccccccaa cgcttcgacc cggccgtact tcttggtcaa tcccgagacc
      481 tctatggaat tcaccgtttg tcctctttcc cttcgagacc ccgcaagaag atgccgacca
      541 ccgcttctat gtgctcctcg tcggtgaggc cgtcttccct tatggccgcg aagaacatct
      601 tgccccccgc ctgtgggaga agcatcccta tgaacgccct ggcgctggcc cgcacgtcgt
      661 gcggcgtgag cctgcctaac tcgacctggc gcgccatgta actcttcaaa aacccgagca
      721 cccttttgat caccgcgttg ccgaacacct ccgagacctc aggccgcctc atggcctcac
      781 caacgagcag ccgcgccatc ctctgcgtgg cgcgattgtc gaacgtcgag aggtacctcc
      841 tggcgatgtt cggtaacacc tcttccggtg gaaggttgag gagcctggca gggtcccgca
      901 ccgcacgcaa tacaggaatc ctggactcca gaacctccct gaaaagagcc tccttgtcag
      961 ggaagtacca gtagatgagc gccggagatt gcaaactcgc cgtccgcgca atactcttga
     1021 tcgtcgcccc cttgaagccc ttcgcagaga actcctcgaa agccgcctcc aggatctgcg
     1081 cccgccggtc cgtctcctca gccaagatac tcctttattg atcgtttaat caaccactat
     1141 actcttgata gaacgttcag tcaagagccg gtggaaacga gtagtagttt cggacgtata
     1201 cctcatcgaa gaggtagtcg acagcgagga gtactcatgc gcaatcctat actggctgcc
     1261 atcctcagcc tcattgtcgc ggggctcggc cagatctaca acggccagat cggcaaaggt
     1321 gtgatcttca tcgtcatcca gcttataaac ggcgcgctga cagccgttct tatcggctgg
     1381 atcctactcc cgatcgtggg cctgtgggca atgatagacg cgtacatgac cgcgaagcgc
     1441 aacaacgagc gctacagctt caggtaatat ctgtcccgtg gacagacgag acctgctaac
     1501 actagagagc gccgtcacgg ccattgaaga ggcggcctcg gccgtcgccc gcgaggtcga
     1561 gcgcgaccgg ctgcgcgaga cctccctggc ccgcctctcc accgtcgaag ccgaactcca
     1621 cagaagccag ctcgcgctag agaagatcat ccaggaagag acggcgtaga gcatgtcagc
     1681 aattcagcac gctacgccct tcgggcttcg cttgtcagca cggcggcttc gccgcctttc
     1741 agcaactcag ccaggctcgc gcacgcgaat gctgaccagc taattcgcac atccctcggt
     1801 gctgacgggg gttgtacggt cggcggccac ccggaggtga cggtggacta ttctggaggg
     1861 aattccgtag cctgagttgt ccgagccggt gcggctggcg aagatggtcg agacgacccg
     1921 cccgtactcg ttgacggcgg ggccgcccga gttgccgggc ctcacgtata cgcggaagct
     1981 ggtgacgacg cgctccaccg ggccactgtc gtaggcgtcg ctggagatga cgcgttgcgt
     2041 cgtacccgta cgggcaggct cgatgtcgag cggaccgttt tcgggaaacc cgatcacggc
     2101 ggcgtctttg ccgggcttgg gtgctgcgag gtgcagcggc gtcaggccaa gaccgtttac
     2161 gcgcaggatg gctatgtcgt tcttctcgtc gaagactacg acctttgcag gcaggcgccg
     2221 ttccgtcgcc acgacctgta catgggtctt cgtctccccg gcgacaacgt gggcgttagt
     2281 gacgaccagg ttccggcccg cgacccagcc cgacccctct acgccgtagc cgcaggctat
     2341 gcctctcaca cgcagagtac tcgcgctcgc cgcctgcacg tcgggatctc cgacgatgct
     2401 tccctctggg gctgatacct ctggttcggg gcctcggatt tgaggcagc
//
LOCUS       DATMJA010000143         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_729165, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000143 DATMJA010000000
VERSION     DATMJA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729165"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VNT12_03330"
     CDS             complement(<1..849)
                     /locus_tag="VNT12_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWI45179.1"
                     /translation="MAKPVTMPQLGESVTEGTITRWLKSEGDEVEIDEPLAEVDTDKV
                     NAELPSPVAGKIEKFLVSEGSTVDVGTEIALVAGAGEDDSPAADAPPQENTAAEAPTE
                     EFPASGTEAQPVAQGTERQRATARADSDGNGRGEIEDAETLRLTRSSPVVRRLAEEHG
                     VEIAEIPGTGTGGRVTRKDIEGYIEEQEEREEYAPPGLQEEREAFAEPESQVERVPVY
                     EGDRVEDLTGIRRAIANRMSASKREAPHAWTMVEVDVSGLAALREREKAAFAEREGMK
                     LTYLPFI"
     gene            complement(861..1847)
                     /locus_tag="VNT12_03335"
     CDS             complement(861..1847)
                     /locus_tag="VNT12_03335"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWI45180.1"
                     /translation="MATKNLLQAIHEGLAEEMRSDESVMMLGEDVGRAGGVFRVTQGL
                     QEEFGAARVMDTPLAESLIVGSAIGLSVNGMRPVAEIQFADFIPPAFDQIVNEAAKFH
                     YRSNGDWNCPITIRAPYGVVPGGALYHSQSVEAWFCNTPGLKVVAPTFPADAKAMIKS
                     AIRDPNPVLFLEHKRTYRLMKEEVPDEDHEVPLGEARVHREGTDITVVAYGLMLYMAL
                     EVAQKLSEEHGIETEVVEPRTLYPLDKEKILDSAKKTGKVLVISEANLTGSVSGEIAA
                     IVAREAFEWLDGPVMRLGAPDVPAAAFAKPMMDRFLPSAEKIEEAMLELARY"
BASE COUNT          311 a          679 c          593 g          436 t
ORIGIN      
        1 tatgaacggc aggtaggtta gcttcatgcc ttctcgctcg gcgaacgcgg ccttctcccg
       61 ctcgcgcaag gcggcgagcc cactcacgtc cacctcgacc atggtccagg cgtgcggggc
      121 ctcgcgcttg ctcgctgaca tgcggttggc aatggccctc cggatgccgg tcaggtcctc
      181 gacgcggtcg ccctcgtaga caggcacgcg ttcgacctgc gactcgggtt ccgcgaaggc
      241 ttcccgctct tcttgtagtc caggcggcgc gtactcttcg cgctcctcct gttcctctat
      301 gtagccttcg atgtccttcc tggttacgcg ccctcccgtg cccgtacccg gaatctccgc
      361 tatctccacg ccgtgctcct cggcgagacg tcggacgaca ggtgaggagc gggtgagccg
      421 caaggtctcg gcgtcctcga tctcacctcg cccgttgcca tcgctgtccg cgcgggcggt
      481 agctcgctgt ctctcagtac cctgtgcgac gggctgagcc tcggtgcctg aggctgggaa
      541 ctcctccgtg ggagcctcgg cggctgtgtt ctcctgtgga ggggcgtcgg cggcgggcga
      601 atcgtcctcg ccggcgccgg cgacgagcgc gatctcggtc cccacgtcga ccgtcgagcc
      661 ttcggagacg aggaacttct cgatcttgcc agccacaggc gagggcaact cggcgttgac
      721 cttgtccgta tctacctcgg cgaggggctc atctatctcc acctcgtcgc cttctgactt
      781 gagccagcgg gttatagtgc cctctgtcac gctctcaccg agttgcggca tcgttaccgg
      841 tttagccacg actccctcct ctagtagcgc gccagctcga gcatggcttc ttctatcttc
      901 tccgccgaag gtaagaagcg atccatcatc ggcttcgcga aagccgccgc gggcacgtcg
      961 ggcgcgccga ggcgcatcac gggcccatcg agccactcga acgcctcgcg cgccacgatg
     1021 gccgctatct ctcccgaaac cgatcccgtc aggttggcct cggagatcac cagtactttg
     1081 cccgtcttct tcgccgagtc caggatcttc tccttgtcca gcggatacag ggttcttggc
     1141 tctacgacct cggtctctat accatgctcc tcggagagtt tctgggccac ctctaaggcc
     1201 atgtagagca tgaggccgta ggcgacgacc gtgatatccg tgccttcgcg gtggacgcgg
     1261 gcctcgccga gggggacttc atgatcttcg tcgggaacct cctctttcat gaggcggtag
     1321 gtacgtttgt gttcgaggaa aagtacgggg ttgggatctc gaatggcgct cttgatcata
     1381 gccttggcgt cggcaggaaa cgtgggcgcg accaccttga ggcctggtgt attgcagaac
     1441 caggcttcga cgctctggga atggtagagg gcgcctcctg gcacgacacc gtagggagcc
     1501 ctgatggtta ttgggcaatt ccagtcgccg ttcgagcggt agtggaactt ggctgcttcg
     1561 ttgacgatct ggtcgaacgc cggcgggatg aagtcagcga actgtatctc ggccacgggg
     1621 cgcatcccat tcaccgagag gcctatggcc gagcccacga tcaggctctc agccagcggc
     1681 gtgtccatca cccttgcagc accgaattcc tcctgcaacc cttgcgtaac cctgaaaaca
     1741 ccgccggccc tgcctacgtc ctccccgagc atcattaccg actcatccga gcgcatctcc
     1801 tcggcgagcc cctcgtggat cgcctgtagc agattcttcg tcgccaccgg aacccctaag
     1861 cctccgcgta gacgccggag agtacgtctt cagggtcggc gaacggggcg tcctcagcgt
     1921 atttgctcgc ctctgcgact tcagtcttta tgcgctccga gatctcatcc ctctccgact
     1981 cgtccaggac gtcgttctcc atcaggtact tctcgaagc
//
LOCUS       DATMJA010000144         3750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_314456, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000144 DATMJA010000000
VERSION     DATMJA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314456"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="thiL"
                     /locus_tag="VNT12_03340"
     CDS             complement(<1..827)
                     /gene="thiL"
                     /locus_tag="VNT12_03340"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWI45181.1"
                     /translation="MNEFDVIRRISALLPPAPPEVLVPLGDDCAVLEIGDSTWVAASD
                     MLVSGHHFKDWATPEDVGYKAVAVNASDVAAMGGTPRFVLASGGAPDPETALRCSEGV
                     LEACERFGVYPLGGDTTRADALTVDVAILGELATQPVLRSGASPGDLLAVTGELGASA
                     AGLLALERGMTGPERLMRRCLRPEPRLGAGRLVARLGATAMIDLSDGLASDVRHLCDR
                     SGVGCTVDLDLLPVKDDTRELTRSLERDPEILAATGGEDYELLICAPGPVLDSLTNSV
                     "
     gene            complement(824..1048)
                     /locus_tag="VNT12_03345"
     CDS             complement(824..1048)
                     /locus_tag="VNT12_03345"
                     /inference="COORDINATES: protein motif:HMM:NF033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HWI45182.1"
                     /translation="MGIVLLVAAVLFWVAAPTVLLTPLSTAQKAWTSSAFLVLGEVAF
                     WVAAVALGREVFWRYRRYLDPRDWLGKERH"
     gene            complement(1120..1992)
                     /locus_tag="VNT12_03350"
     CDS             complement(1120..1992)
                     /locus_tag="VNT12_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI45183.1"
                     /translation="MKADLVSGLLPVREDGRMLLLQRPTGTWEPPAGRLQPGEGFEEG
                     AVRELYEETGILVSPQRIIASWVGDAASGRRLASVTYAGKTDGAEVRLSEEHLDHRWV
                     TLGEWMSLPSWWSHENIQRIAGPVGAVRIMQAVREPPPPPLRGDTGVVSANLGAGAVL
                     VDLGGIEPRALLLRRRKPPVGLWENPGGMLEEGEDFEACASRELYEETGVLAGIEDPW
                     WARVEPWRNPDDQELYAGVGFLARHQGGEIAPEAAAHDASMWVTETGWRSLRTWYTRR
                     ESDVLWKAIREMQR"
     gene            complement(2095..2862)
                     /locus_tag="VNT12_03355"
     CDS             complement(2095..2862)
                     /locus_tag="VNT12_03355"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HWI45184.1"
                     /translation="MFAAFRPETVLSRTEHRPYPVSSGPWVLGMGWRDLLFMHWPVEV
                     DALRSLVPPALSIDTFEGSAWLGVVPFDMTGVRPRFLPAVPGVSNFPEINLRTYVTAE
                     GKPGIWFFSLDAHSRVAVRLARATFHLPYFDAEMSCHASDDEVDYRSLRTHRGAPRAE
                     FAARYSPVGEPFESERGSIENFLTERYCLYSADRRGNVRRGEVHHHLWPLQPAEAEVS
                     TLAMTQQIGLKLLEAAPILHFSKRLDVLAWLPRKIGS"
     gene            complement(2912..3556)
                     /locus_tag="VNT12_03360"
     CDS             complement(2912..3556)
                     /locus_tag="VNT12_03360"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HWI45185.1"
                     /translation="MRAAIFLNGSPDSAGLLRRLARRANLVVAADGGARYALGAGVVP
                     ELVVGDMDSLGEELAREVERRGASLERHPARKDKMDGQLAVLAARERGATAADLLCAV
                     GGRLGAIFAVPQILLAAERIGLRSTVVADRGRIFVIEAGSRTIQGDSQDSVSIFPLSG
                     PATGVTLVSMEYPLENASLEPGDTLGFHNELIGSKASVSVEEGALLVVQETGEP"
     gene            complement(3558..>3750)
                     /locus_tag="VNT12_03365"
     CDS             complement(3558..>3750)
                     /locus_tag="VNT12_03365"
                     /inference="COORDINATES: protein motif:HMM:NF021062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HWI45186.1"
                     /translation="GVIFFASYAPEGWNPYLYSAAYNAAYLAPSLGIALVVTVVLLKA
                     LEVAQPSPRQLRYRSPKTS"
BASE COUNT          668 a         1243 c         1192 g          647 t
ORIGIN      
        1 acgctgttcg tcagcgagtc caggacaggc ccgggggcac agatcaggag ctcgtaatct
       61 tcgcctcctg ttgccgctag gatctcgggg tcgcgttcga gggaacgtgt gagctctctg
      121 gtatcgtctt tgacgggcag gagatccagg tccactgtgc atcccacccc gctccggtca
      181 cacaggtggc gtacgtccga agccaggccg tcggagaggt ctatcatggc ggtggcgccg
      241 agcctagcga cgaggcgccc ggccccgagc ctcggttcgg gccgcaagca cctgcgcatg
      301 agacgctccg ggccggtcat gcctctctcg agggcgagta gtccggccgc cgaggcgccg
      361 agctcgcccg tgaccgccag gaggtcgccg gggctcgctc ccgagcgcag taccggctgg
      421 gtggcgagct caccgaggat agccacgtcc accgtgaggg catccgcgcg ggtcgtatcc
      481 cccccgagcg gatagacgcc gaacctctcg caggcctcca agacaccctc agagcagcgc
      541 agcgcggtct cagggtcggg cgctcctcca gaggccagaa cgaacctcgg ggttcccccc
      601 atcgccgcca cgtccgaggc gttgaccgcg acggccttgt agccgacgtc ctcgggcgta
      661 gcccagtctt tgaaatggtg gccagagacg agcatatcgg aggcggccac ccacgtactg
      721 tccccgatct ctagaactgc gcagtcgtcc ccgaggggca cgagcacctc ggggggcgcg
      781 ggaggcagga gggctgagat cctgcgtata acatcgaact cgttcaatga cgctctttgc
      841 ccaaccagtc gcgaggatcg aggtatctcc tgtagcgcca gaatacctca cggccgagcg
      901 ccacggcagc gacccagaac gctacctctc ccaagaccag aaaggccgaa ctcgtccagg
      961 ccttctgcgc cgtggagagc ggagtcagca gtacggtcgg ggccgccacc cagaacagca
     1021 cagcggccac gagcaatact attcccacga gccgcgtcag gccagccctt ttcgaaacgc
     1081 gaggttcctg gcccgttatt tgcctgttct cctgttcgtc tatcgctgca tctccctgat
     1141 ggctttccag agcacgtctg actctcgtct cgtataccag gtacgcagcg agcgccaccc
     1201 ggtttccgta acccacatag aggcgtcgtg ggccgcggct tcaggagcaa tctctcctcc
     1261 ctgatgcctc gccagaaagc ccacgccggc atagagctcc tggtcatcag ggttgcgcca
     1321 gggctcgacc ctggcccacc aggggtcttc gataccggcc agcacgccgg tctcttcgta
     1381 gagctcgcgg ctggcgcagg cttcgaaatc ctcgccttct tcgagcatgc cgccagggtt
     1441 ctcccagagc ccaacgggcg gtttgcggcg cctgagcagg agcgcccgcg gctcgatgcc
     1501 tccaagatcc acgagtacag ccccggcccc gaggttggca gagactactc cggtgtcccc
     1561 gcgtaagggc ggtgggggcg gttcgcgaac cgcctgcatt atgcgaaccg ctcctacagg
     1621 tccggctatg cgctggatgt tctcgtggct ccaccagctc ggtaacgaca tccactcccc
     1681 gagggtcacc cacctgtggt cgagatgctc ctcggagaga cggacctctg ctccatcagt
     1741 ctttccggcg taggtgacgg aggccagacg tctaccgctc gccgcatcgc cgacccagga
     1801 ggcgatgatg cgttggggag agaccaggat gccggtctct tcgtagagct cgcggacggc
     1861 cccttcctcg aagccctcgc ccggctgaag acgtccggcg ggcggttccc aggtaccagt
     1921 gggacgctgg agcaggagca tgcggccgtc ctccctgacg ggtaaaagtc cagagacgag
     1981 atccgccttc aaagcgcgcc cgtagggaag atttcgcgtc tgctctccat tcggcaagta
     2041 tatggtccgc gagggcggtt cgcgaaccgc ccttaccacg cgaaccgtcc cggcttaaga
     2101 gcctatcttt cgcggtagcc acgcgaggac gtccaggcgt ttggagaagt gaaggatcgg
     2161 ggcagcttcg agcagcttga ggcctatctg ctgggtcatc gcgagcgtgc tgacctcggc
     2221 ctcggcgggc tggaggggcc atagatggtg gtggacttct ccgcggcgga cgttgcccct
     2281 cctgtccgcg ctgtacaggc agtagcgctc ggtcagaaag ttctcgatgg accccctctc
     2341 gctctcgaag ggttccccca cagggctgta gcgggccgcg aactccgccc gcggcgcgcc
     2401 cctatgcgtc ctcaggcttc tgtagtcgac ctcgtcgtcc gaggcgtggc aggacatctc
     2461 ggcgtcgaag taaggaaggt ggaatgtggc gcgggcgagg cggacggcta cgcggctgtg
     2521 ggcgtccagg gagaagaacc agatgcccgg cttgccttcc gccgtcacgt aggtgcggag
     2581 gtttatctcg gggaaattag aaacccccgg cacagcgggt agaaaacggg gcctcacgcc
     2641 ggtcatatcg aagggcacga cgccgagcca tgcgctgcct tcgaaggtat cgatgctcaa
     2701 ggccggcggc accagagacc tgagcgcgtc cacttcgaca ggccagtgca tgaagagcag
     2761 gtcccgccag cccatcccga gtacccaggg accagacgaa acggggtagg ggcggtgctc
     2821 tgtcctgctc agaacagtct cgggtcgaaa agccgcaaac ataagcaacg gttatcttaa
     2881 gatgagcaag cgcgaaggtc cgtataccct gtcacggttc tccggtctct tgtaccacga
     2941 ggagtgcgcc ttcctctacg ctcacgctgg ctttgctgcc gatcaactcg ttgtggaatc
     3001 ccagggtgtc tccaggctcc aggctggcgt tctccagcgg atactccata cttaccaggg
     3061 tgacgccggt ggcaggacct gagaggggga agatcgaaac gctatcctgc gaatctcctt
     3121 gtatcgtcct ggagccggcc tcgatcacga aaatccgccc tcggtcggcc acgaccgtgg
     3181 aacgcaggcc gatgcgctcg gcggcgagaa ggatctgtgg gaccgcaaag atggccccta
     3241 acctcccgcc cacggcgcag aggaggtcgg ctgcggtcgc tccccgctcc ctggcagcca
     3301 gtacagcgag ctggccgtcc atcttgtctt tcctcgccgg atggcgctcc agggaggccc
     3361 cacgcctctc tacttctcgc gccagctctt ctcccaacga gtccatatcc ccgacgacga
     3421 gttcaggcac aaccccggcc ccgagcgcgt agcgggcccc accgtcagcg gcgactacca
     3481 ggttcgcgcg tcgggcgagg cgcctgagca ggcccgccga gtcgggagag ccgttcagga
     3541 agattgcggc ccgcacgcta ggaggttttc ggactccggt agcgcagttg tcttggcgaa
     3601 ggctgcgcta cttccagcgc cttcagcaac actaccgtga ccacgagtgc gatgcccagg
     3661 ctcggggcca ggtaagcagc gttgtacgcc gctgaataga ggtatgggtt ccagccctcc
     3721 ggcgcgtagg aggcgaagaa gatcacaccc
//
LOCUS       DATMJA010000145         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_847709, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000145 DATMJA010000000
VERSION     DATMJA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847709"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VNT12_03370"
     CDS             complement(<1..929)
                     /locus_tag="VNT12_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HWI45187.1"
                     /translation="MMPQTTGIHHVTAISGRPQRNVDFYAGTLGLRLVKKTVNFDDPE
                     TYHLYYGDEAGSPGTIMTFFPWAHAPGGRIGAGQLVVTSFSIPTTSLGYWTERLVEKG
                     VRFERPRDRFGDTVLTFEDPDRLRIELVAAEDGRPERSRGPVQAEHSVRGFHHVALAV
                     EPPERTARLMTDTLGFRRVDEAEGRIRLASGKGGPGDLVDVVNAAGFPHGSMGVGTVH
                     HVAFRVPDEETQLALREEVAALGYSVTPVLDRNYFRSIYFREPGGVLFEIATDPPGFA
                     VDEDPEHLGEDLKLPPWLETRRDRLEEVLPPLRL"
     gene            complement(1093..1959)
                     /locus_tag="VNT12_03375"
     CDS             complement(1093..1959)
                     /locus_tag="VNT12_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWI45188.1"
                     /translation="MLPERILLPTDGSEDAIRATEAASDLAKRCGAELHVVHVWHDVR
                     GFAHDFVKRELRRQGQEILDEQVEKIRASGGEVTKAYLRRGRTSNEVIALCKEIDADL
                     LVVGSRGLGTVRRILMGSQSEEIVHHAQVPVLVLTGAEDFWHPARIVIGEDFSDDARK
                     AGELAAAIGKLYSAEGLLVYSHPDLPEVPPGEARSTVQNFTEMRERDESMLEERASEL
                     EEILGSRPATRISGGYPANVLLEASQEVQPSLVALGSRGLAGLIRTRLGSVSTKVVTA
                     ARGPVLVYPHVD"
     gene            complement(2018..>2548)
                     /locus_tag="VNT12_03380"
     CDS             complement(2018..>2548)
                     /locus_tag="VNT12_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI45189.1"
                     /translation="VLQEPFLFSGTIAENIGYGRSGDPSEATREEVEDAARIVNANDF
                     ITALPEGYDTVLGEGGGALSHGQRQLLSFARAVLTDPRILILDEATSNIDTRTEAIIQ
                     KALATLLEGRTSIVIAHRLSTIRNADLILVIEDGNIAERGSHAALIEENGLYADLYRR
                     QFREPVGRKILTRKDG"
BASE COUNT          427 a          822 c          799 g          500 t
ORIGIN      
        1 aggcgcagtg gtgggaggac ctcttccagc ctgtcgcggc gggtctcgag ccatggcggt
       61 agcttgaggt cttcgcccag gtgttcgggg tcttcgtcta ccgcgaaacc tgggggatcg
      121 gtggcgatct cgaacagcac cccgccaggc tcgcggaagt agatcgaacg gaagtaattg
      181 cggtcaagca caggcgtaac gctgtagccg agcgcggcta cctcctcacg cagcgcgagc
      241 tgagtctcct cgtcggggac ccggaacgcg acgtggtgca cggtgcctac gcccatgctt
      301 ccatggggga agccggccgc gttcaccacg tcaaccagat ctcctgggcc acctttgcct
      361 gaggcgagcc tgatcctacc ctccgcctcg tcgacccgcc ggaagcccag agtatctgtc
      421 atcaacctgg cggtacgttc tggcggctcg acggcgaggg cgacgtggtg gaagcctctg
      481 acagagtgct cggcctggac cggaccgcgg gaccgttctg ggcgtccgtc ttccgcggcc
      541 accagctcta tgcgcagacg gtccgggtcc tcgaaagtga ggactgtatc cccgaagcgg
      601 tcgcggggtc tctcgaagcg gacacccttc tccaccaggc gctccgtcca gtagcctagt
      661 gaggtggtcg ggatggagaa cgaggtaacg acgagctggc cggcccctat ccgtccccct
      721 ggcgcgtgcg cccacgggaa gaacgtcatg atggtgccag ggctacctgc ttcgtcgccg
      781 tagtagaggt ggtaagtctc gggatcgtcg aagttgacgg tcttcttgac cagcctgagg
      841 cccagagttc cggcgtagaa gtccacgttg cgctgcggcc tgcccgagat ggcggttacg
      901 tggtggattc ctgtagtctg cggcatcatg gtgctcctct tcgaggtttc atcggacgcg
      961 ggatcatctt agcaccggcg aacctggcgg cgattcggcc gcgaagaggt gagatgtacc
     1021 ccagcccata aggccggaag gcaggtgttt ctgtcgagtg gcctaccctg caaagccctg
     1081 aaacctataa tctcaatcaa cgtgcggata gaccaggacg ggtcctcttg cggcggtcac
     1141 gaccttggtc gagacgctcc ctagcctggt ccgaatcagt ccggccaggc ccctgcttcc
     1201 gagggctacg agcgagggtt gcacctcctg ggaggcttcg aggagcacgt tggccgggta
     1261 gccgccggag atcctggtcg caggcctgct ccccagtatt tcctccagct cactggcacg
     1321 ctcttcgagc atgctttcat cccgctcgcg catctccgtg aagttctgga ccgtggacct
     1381 cgcctcgccg ggaggaacct cgggcagatc gggatgagag tagacaagca gaccctcggc
     1441 actgtagagc ttgccgatgg cggcggcgag ctctcctgcc ttgcgcgcgt cgtcggagaa
     1501 gtcctcgccg atgacgatcc gggccggatg ccagaaatct tctgcgcccg taagtacgag
     1561 gacggggacc tgggcgtggt gcacgatctc ctcggactgg ctgcccatca ggatgcgccg
     1621 caccgttccc agtccgcggc tgccgacgac caggagatca gcgtcgatct ccttgcagag
     1681 ggcgatgact tcgttggagg tgcgccccct cctgagatac gccttcgtga cttccccacc
     1741 ggaggccctg atcttctcta cctgttcgtc cagtatctcc tggccctggc gcctcaactc
     1801 cctcttgacg aagtcgtggg cgaacccccg cacatcgtgc cagacgtgaa cgacatgaag
     1861 ctcggccccg catctcttgg ccagatccga ggccgcctct gtcgccctga tagcgtcctc
     1921 cgagccgtcg gtaggcagca gtattctctc cggaagcacg ctcaactgaa actcctctca
     1981 tcctccatgt tatctaacgc cagtccgaat aataccctca gccgtccttt cgggtcagga
     2041 tcttccgccc gacgggctcg cgaaactggc ggcggtagag gtccgcatac aggccgttct
     2101 cctctatcag cgcggcatgg ctgccgcgct ccgcgatgtt gccatcctct atgaccagga
     2161 tgaggtcggc gttcctgata gtgctcaggc ggtgggcgat gacgatgctc gttctgccct
     2221 ccagcagcgt ggccagggcc ttctggatga tcgcttccgt gcgcgtatct atattgctcg
     2281 tggcctcgtc cagaatgagg atgcgcgggt ctgtcaggac cgccctggca aaggagagta
     2341 actggcgctg cccgtggctc aatgccccgc ctccctcacc caagactgtg tcgtagcctt
     2401 cgggaagagc cgtgatgaag tcgttggcgt tgacgatacg ggccgcgtcc tccacctcct
     2461 cgcgcgtcgc ctccgagggg tcgccggaac ggccgtagcc gatgttctcc gcaatggtgc
     2521 ccgagaacag gaaaggctcc tggagcac
//
LOCUS       DATMJA010000146         3048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_196512, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000146 DATMJA010000000
VERSION     DATMJA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196512"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VNT12_03385"
     CDS             <1..480
                     /locus_tag="VNT12_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HWI45190.1"
                     /translation="GRSGVEALENGREDAFPRPIVGNAIPQIGGVGDDGYTTEESKMR
                     EETRKILSLPDLPVFATAVRIPVHTGHAECIYVETERDADLPALLEALGAASGVEVSD
                     DFPTPLEVAGEDRTYVGRVRVEGRVIQYWCVADNLLKGAATNAVQIAESLVDVHARA"
     gene            584..856
                     /locus_tag="VNT12_03390"
     CDS             584..856
                     /locus_tag="VNT12_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45191.1"
                     /translation="MAQGEKRSFWDRLFGRNQPSAREERVVEYIIHRLGEGAHLGDVV
                     KEEYVRRNASPQEIEEICADPRLVEAARQHMRQDFDSGELDPDKRP"
     gene            937..1242
                     /locus_tag="VNT12_03395"
     CDS             937..1242
                     /locus_tag="VNT12_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWI45192.1"
                     /translation="MPIYEYKCENGHVFDVMQKLSEDPLSSCVECGAPVRKVLHPVSI
                     SFKGSGFYSTDYSKKGPKEVAQKDTSKSTNGDNGSRSDEKKSKEKPAEPSSSSGKKD"
     gene            1247..1495
                     /locus_tag="VNT12_03400"
     CDS             1247..1495
                     /locus_tag="VNT12_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45193.1"
                     /translation="MARGAGLPQSRDRQEGILRRALRAVVLGITLLFVVSALAVSCAL
                     VVYTAWRHEFSHGLFALLFAVLSAQIALNWFLWKDDEE"
     gene            1844..>3048
                     /gene="dnaE"
                     /locus_tag="VNT12_03405"
     CDS             1844..>3048
                     /gene="dnaE"
                     /locus_tag="VNT12_03405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWI45194.1"
                     /translation="MAVTSGGAFVHLHCHSEYSMLDGASRIGDLISFAKDQGMPGLAL
                     TDHGVMYGAVKLYKEAKDAGIKPLMGCEVYVTADRNDRSRAPYYHLTLIARTAEGYRN
                     LMKLSTCGFLEGFYYKPRVDMEMMRKYGRGIICLSGCLSAEVPTRILEGRPEEARRLV
                     LAYAEIFDAVYLEMQDHLEIPDQKRVNEGLVRLHAETGLDLVATNDSHYTTRNDARMH
                     DVLLCIGTGRFYNDQNRMKFSGQEFYVKPAEEMAALFPNHPEALENTIKVLKSVEDVG
                     IELGKTRLPNFPKPEGYTTDQYLREQCERGLARRYGGRAKSREVLGRLEFELETIEKM
                     GFADYFLIVWDFVKYAKDRKIAVGPGRGSAAGSIVAYALEITDLDPLEYSLLFERFLN
                     PDRINMPDV"
BASE COUNT          651 a          876 c          991 g          530 t
ORIGIN      
        1 ggacgcagcg gcgtcgaggc gttggagaac ggcagggagg acgcgttccc aaggcccata
       61 gtggggaacg ccatacccca gatcgggggt gtcggcgacg acggatacac gaccgaggag
      121 tccaagatga gggaggagac acgcaagatt ctctctctac ccgaccttcc cgtcttcgct
      181 accgccgtca ggattccggt ccacacaggc cacgccgagt gcatctacgt cgagaccgag
      241 cgggatgcag atctacccgc gctgctcgaa gcgctggggg cggcgtctgg cgtcgaggtc
      301 tctgacgact ttccgacgcc gctcgaggtc gccggagagg ataggaccta cgtcgggcgt
      361 gtacgcgtgg aggggcgagt gatccaatac tggtgcgtcg cggataacct gctcaagggc
      421 gcggcgacga acgccgtcca gatagccgaa tcgctcgtcg acgttcacgc gcgggcctag
      481 aggccgttct ggaggcgccg ttcggccctt gctaagcctg tacggagaag ctgcaactcg
      541 ttaaactgcg gtgtgggaag atacccgcgg tgaggagaga gtcatggcac agggcgaaaa
      601 gagatccttc tgggacaggt tgtttggccg caatcagcct tccgcgcggg aggagagggt
      661 ggttgagtac atcatccacc gcctgggcga aggtgcgcac ctgggcgatg tggtaaagga
      721 ggagtacgtg cggcgcaacg cttcgcccca ggagatagag gagatctgcg ccgatcccag
      781 gctcgtcgaa gccgcgcgcc agcacatgag gcaggacttc gactcggggg aactcgaccc
      841 tgacaagcgt ccatgatagc cgcctggtgc ggaggggacc ggaagtctat ctagcctgta
      901 taatctgggt tcacgagtac caaaaatgga ggctggatgc cgatctacga atacaagtgt
      961 gagaacgggc acgtcttcga cgtgatgcag aagctgtccg aagaccccct ctcgtcctgc
     1021 gtcgagtgcg gcgcccctgt caggaaagtc ctccatcccg tcagtatctc gttcaagggc
     1081 tcgggcttct actccacaga ctacagcaag aagggcccga aagaggtcgc acagaaagac
     1141 acctccaagt ccaccaacgg ggacaacggg tctcgctcgg acgagaagaa gagcaaagag
     1201 aagccagccg agccctccag cagctcaggg aaaaaggact gaacccatgg cgcgcggcgc
     1261 cggcctgcca cagagccgag accgccagga aggtatcctc agaagggcac tccgcgcggt
     1321 tgtgctcggg atcacgctcc tcttcgtcgt ctcagccctc gccgtctcct gcgccctcgt
     1381 cgtctacact gcctggcgcc acgagttcag ccacggcctc ttcgccctac tcttcgccgt
     1441 cctcagcgcc cagatagcac tgaactggtt tctgtggaaa gacgacgagg agtgagcact
     1501 tcagctagcc agcacgcgcc ctgtcgggcg cttgtcagca cggcggctgc gccgccttgt
     1561 cagccagaac gtgcgcatgc aagagctgac tagctgaaat gctgacaagt cgcctggcga
     1621 agcctggcga cgtgctgaca agcggaggcc gccaggccgg agcgtgctga aatgcggtcc
     1681 tgaaaagtcc tgcaaaacat gcctcaactt gtggtcgagg tactctggac aacttgtagt
     1741 tgcgtgcctt atagtagccc gcgtcgggta tcgacgggga gaacaggcga ccggcggcgc
     1801 tctttttggg cgtcgccgaa acccgggagt agagggagga aggttggcgg ttacgtcggg
     1861 cggcgctttc gtacacttgc actgtcactc tgagtattcg atgctcgacg gggcgagccg
     1921 tatcggggac ctcatctcct ttgcgaagga ccaggggatg ccagggctcg cgctcacgga
     1981 ccacggtgta atgtacggcg cggtcaagtt atacaaggag gccaaagacg ccggcataaa
     2041 gcccttgatg ggctgcgagg tctacgtcac ggccgaccgg aacgacagga gccgcgcccc
     2101 ttactaccac ctcacgctca tcgccaggac cgcagagggt tacaggaacc tcatgaagct
     2161 ctccacctgc ggatttctgg agggtttcta ctacaagccg cgggtcgaca tggagatgat
     2221 gaggaagtat gggcgtggga tcatctgcct ctcaggttgc ctttcggcag aggtgcctac
     2281 caggatactg gaggggcgtc cggaggaagc gcgcaggctg gtactagcgt acgcggagat
     2341 cttcgacgcc gtctacctcg agatgcagga tcacctggag ataccggatc agaagcgcgt
     2401 gaacgaaggg ctggtaaggt tgcacgcgga gaccggactg gacctcgtcg ctaccaacga
     2461 ttcccactac accacgagaa acgatgccag gatgcacgac gtgttgctgt gcatcgggac
     2521 cggcaggttc tacaacgatc agaaccgcat gaaattcagc gggcaggagt tctacgtcaa
     2581 gcccgcggaa gagatggcgg cgctcttccc gaatcacccg gaggcgctgg agaatacgat
     2641 caaagtcctc aagagcgtcg aagacgtcgg gatcgagctg ggtaagacgc gactaccgaa
     2701 cttcccgaag ccagaagggt acaccaccga ccagtacctg agggagcagt gcgaacgcgg
     2761 cctcgcgagg cgttacggtg ggcgggcgaa gagccgggag gtgctcggca ggcttgagtt
     2821 cgagttggag accattgaga agatgggctt cgcggactac ttcctgatcg tctgggattt
     2881 cgtcaagtat gcgaaggaca ggaagatcgc ggtcgggccc ggacggggat ccgctgccgg
     2941 ctccatcgtc gcctacgcgc tggagataac ggacctcgat cccttggaat actctctgct
     3001 cttcgagcgc tttttgaacc ctgaccgcat caatatgcct gatgtgga
//
LOCUS       DATMJA010000147         4804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_255499, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000147 DATMJA010000000
VERSION     DATMJA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255499"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1257
                     /locus_tag="VNT12_03410"
     CDS             73..1257
                     /locus_tag="VNT12_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWI45195.1"
                     /translation="MLEQERLVESAALGGRATARLTNPYVRDEFGLETMDLNVGPQHP
                     AMHGLLRLILELDGETIVRCDTVMGYLHRCQEKIAENRMYPAVIPMTDRLDYFANMHM
                     EHGYCLAVEDLMGLEIPPRADAIRVLMSELMRLASHMPSIGFLMLELGAFTPILYAFR
                     ERERIQDIFEAVTGARMMFHYIRIGGVKADLPEDIPERIWEYVESLEANLDRDFVDLI
                     QGNEIFITRTRGVGKVTQEEAVEMGLTGPPLRCTGVDFDIRRDYPYSWYPNVEFDVIT
                     DTAGDAEAAYRVRVGEIRQSIRIVKQILESMPEGAVMGDVPRRIRPPEGEGFGRVESP
                     RGEFAVHVVSDGSDVPYRVHYRDPSFVNMQFLQEILPGHYLPDVMPIMGIVDPVSGGW
                     DK"
     gene            1254..2273
                     /locus_tag="VNT12_03415"
     CDS             1254..2273
                     /locus_tag="VNT12_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HWI45196.1"
                     /translation="MISTLLEYYNQEPWRFLSAFGAILFLTLNIAAILTMSERKVAGY
                     IQGRYGPNRVWPRGLLQPAADVVKLFTKENLSPTGSDLWIFLAAPIAMFIPAATIWLV
                     VPFAPGVVIADLNIALLFFISITSIGALGVIMAGYGSRSNFSLLGALRGAGQLISYEV
                     PLILSLLGITILTGSMSLVDVVDAQAGGFWNWYVWFQMPMFIAFYVAGIAEVKRIPFD
                     LPEGESEIVGGFMVEYSGMTWALIQASEFALMGLMSAIGATLFFGGWQPPLPFLDLGI
                     FNWIWFGLKTALIMFTFLWIRWTLPRLRMDQLMDLGWKVLVPLTIVWLFVTAGAVLAF
                     PSFFG"
     gene            2284..2796
                     /locus_tag="VNT12_03420"
     CDS             2284..2796
                     /locus_tag="VNT12_03420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWI45197.1"
                     /translation="MPQIGQGVLKGMGITLRHLFSQKITRQYPENKRMLPERSRGLLT
                     VDMDRCIACLQCMRVCPDHCISIEMEKRDFDGSGKAKPYAVGFVIDDSRCMYCSLCVE
                     VCPVNCIYHTEEFEAQAYNRLELARQFGERPVDPTVDPKPAVKKKKKATAKKAPRTVE
                     RPVTEKDDAA"
     gene            2793..3329
                     /locus_tag="VNT12_03425"
     CDS             2793..3329
                     /locus_tag="VNT12_03425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWI45198.1"
                     /translation="MTVAFAFLAGMTLVSALGVVISRNVVHSALFMSGSFLGIAGYFV
                     MLNAPALAAFQVLIYVGAVTVLILFGVMFTQKPQVRRYRTIINRQFWAGLFVATGVGA
                     FLAYVLSAEDWGGVEAGNGPRQVAALGRDLLGVSQGAEIFTLLFEVASVLLLVAIVAA
                     IVISHRKSGGRVDQGVEE"
     gene            3341..3691
                     /gene="nuoK"
                     /locus_tag="VNT12_03430"
     CDS             3341..3691
                     /gene="nuoK"
                     /locus_tag="VNT12_03430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWI45199.1"
                     /translation="MPPQVQQVLDALTATPPLEGYLVVGAFMFAIGAWGALIRRNAVV
                     IFMCVELMINAVNLTLVAFADYLPNAGGLGVSYAVLVIAIAAGEIAVGLAIVLAVFRS
                     RRTVNVDEVTSMRG"
     gene            3702..>4804
                     /locus_tag="VNT12_03435"
     CDS             3702..>4804
                     /locus_tag="VNT12_03435"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWI45200.1"
                     /translation="MQTVGQQLIITAIPTLPALVFLVLVLTGRLLKENAQYVAILGVT
                     ISLVFSVFALLFVAGIVPGGGAPIEFSVNWIDLGQGGSFPMGVYIDGLAAVMLVVVCF
                     VSLMIQLYSGAFMEGDKRFAWYYALLNLFTASMLGLVLAPNFIQLYVFWELVGLCSYL
                     LVGHWFEKPSARDAAIKAFVVTRIGDAGLFVGIIIFWATTGSTAFTDISEAAQAGFIG
                     GSLFTTAVILVFIGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVART
                     YDIFVQSPTAMLVVAYIGGFTALMAATMALVKKDIKRVIAYSTVSQLGYMMLGLGLGS
                     YTAGVFHLYNHAFFKALLFLCAGSIIYAMDSYNL"
BASE COUNT          835 a         1457 c         1512 g         1000 t
ORIGIN      
        1 ttcgatcacc atccactgct caagagcttc gagatcaaca caaagcgcac cttcgagggg
       61 ttgaggtaag agatgcttga acaagaacgt ctcgtagaga gcgcggccct gggtggccgg
      121 gccacagcca ggctgacaaa cccttacgtc cgggatgagt tcggcctcga gactatggat
      181 ctgaacgtgg gcccccagca cccggcgatg cacgggctcc tgaggctcat cctcgagctc
      241 gacggggaga cgatcgtgcg ctgtgatact gtcatggggt atctgcatcg ctgccaggag
      301 aagatcgccg agaacaggat gtacccggcc gtcatcccca tgaccgaccg gctcgattac
      361 tttgccaaca tgcacatgga gcacggctac tgcctcgccg tcgaggacct gatggggctc
      421 gagatcccac cgcgggcgga cgcgatacgc gtcctaatga gcgagctcat gcgtctcgca
      481 agccacatgc cgtctatagg cttcctcatg ctggagctcg gggcgttcac gccgatcctg
      541 tacgcgttca gggagcggga gcggattcag gacatcttcg aggcggtcac gggcgccagg
      601 atgatgttcc actacatccg catcggcggt gtcaaggccg acctgcccga ggacatacca
      661 gagaggatct gggagtacgt cgagagcctg gaagcgaacc tggatagaga cttcgtggat
      721 ctcatccagg ggaacgagat cttcattacc cgcacccgcg gcgtcgggaa ggtgacacag
      781 gaggaggcgg tcgagatggg tctcacaggg cctcctttgc gttgcacggg ggtggacttc
      841 gacatcaggc gcgactaccc gtacagttgg tacccgaacg tggagttcga tgtcatcacc
      901 gataccgcgg gcgatgccga ggccgcctac cgggtgaggg tcggagagat caggcagagc
      961 ataaggatcg tcaagcaaat tctggagagc atgccagagg gcgcggttat gggggacgtg
     1021 ccccgccgga tacgtccgcc cgaaggcgaa ggcttcggca gggtcgagag tccgcggggc
     1081 gagttcgcgg tacacgtggt ctccgacggg tctgacgtac cctaccgggt gcactaccgc
     1141 gatccgagct tcgtaaacat gcagttcttg caggagatcc tgccaggcca ctacctgcct
     1201 gacgtgatgc cgatcatggg catcgtggat ccggtctccg gcgggtggga taaatgatca
     1261 gcacgctgct cgaatactat aaccaggagc cctggcgctt cctgagcgct ttcggggcga
     1321 tactgttctt gaccctgaac atagcggcga tcctcacgat gtccgagcga aaggttgccg
     1381 gatacatcca gggacgctac ggacccaacc gcgtctggcc tagggggctt ttgcaaccgg
     1441 cggctgatgt agtcaagctg tttaccaagg agaacctgtc gccgaccggg tcggacctct
     1501 ggatcttcct ggcggcgccc atcgccatgt tcatcccggc ggcgaccatc tggcttgtgg
     1561 tgcccttcgc gccgggggtg gtcatcgccg atctgaacat cgcactgttg ttctttatct
     1621 ccataacgtc cataggggcc ctcggcgtga tcatggccgg ctacggtagc cggtcgaatt
     1681 tctcgctcct gggagccctc aggggcgccg gccagcttat ctcttacgag gtgccgctga
     1741 tcttgagctt gctcgggatc acgatcctca cggggagcat gtcgctcgtg gatgtcgtcg
     1801 acgcccaggc cggcggtttc tggaactggt acgtctggtt ccagatgccg atgttcatcg
     1861 cgttctacgt cgcagggatc gcggaagtaa agcggatccc gttcgacctg cctgaggggg
     1921 agagcgagat cgtcggcggg ttcatggtcg agtattcagg gatgacctgg gctctgatcc
     1981 aggcctccga gtttgccttg atgggcctga tgtcggcgat cggcgcgacg ctgtttttcg
     2041 ggggctggca gccgccgctc ccgttcctgg acctcgggat tttcaactgg atctggttcg
     2101 ggctcaagac cgcgcttatt atgttcacct tcctgtggat ccgctggacc ctgccgcgcc
     2161 tgcgcatgga ccagttgatg gaccttggtt ggaaggtgct cgtgccgctc accattgttt
     2221 ggctgttcgt caccgctggt gcggtgctgg cgttcccgtc gtttttcgga tagaggagag
     2281 cttatgccgc agataggaca gggcgtcttg aaggggatgg ggatcaccct caggcacctc
     2341 ttctcacaga agatcacacg ccagtacccc gagaacaagc gcatgctccc ggagcgcagc
     2401 cgcgggctgc tgaccgtgga catggaccgc tgcatcgcat gcctgcagtg catgagggtc
     2461 tgccccgacc actgcatctc catcgagatg gagaagcgtg acttcgacgg ctcaggcaaa
     2521 gccaagccct acgcggtcgg ctttgtcata gacgactcgc gctgcatgta ctgttccctt
     2581 tgcgtcgagg tctgcccggt caactgcatt taccacaccg aggagttcga agcccaggcc
     2641 tacaaccggc tggagctcgc caggcaattc ggcgagcgtc ccgtcgaccc gacggtggac
     2701 cccaagcccg ccgtcaagaa gaagaagaag gctactgcca agaaagcgcc aaggaccgta
     2761 gagcgtcccg taaccgagaa agacgacgcg gcttgacggt agcgttcgcc ttcctggccg
     2821 ggatgacgct ggtctcggcg ctcggtgtgg tgataagccg caacgtggtg cactcggcgc
     2881 tgtttatgtc tgggtctttc ctcgggatag cggggtactt cgtgatgctc aacgcgccgg
     2941 cgctcgccgc gtttcaggtc ttgatctacg tcggggctgt gacggtcctg atactgttcg
     3001 gggtcatgtt cacccagaag ccgcaggtca gacgctaccg tacgatcatc aaccggcagt
     3061 tctgggccgg cctctttgtg gccaccggcg tcggggcctt ccttgcctac gtgctctctg
     3121 ccgaagactg gggaggggta gaggcgggta acgggccgcg ccaggtcgcc gccctcgggc
     3181 gggacctcct cggcgtcagc cagggagctg agatcttcac cctgctcttc gaggtcgcct
     3241 cggtattgct gctcgtcgcg atcgtcgctg caattgtcat cagccaccgc aagtcaggcg
     3301 gacgtgttga tcagggggtg gaggagtagt gccgccgcag atgccaccgc aggtgcagca
     3361 ggtcctggac gctttgacgg cgacgccgcc gctggaaggt tatctcgttg tgggggcttt
     3421 catgttcgcc ataggggcgt ggggggccct gatccggcgc aacgccgtcg tcatcttcat
     3481 gtgcgtggag cttatgatca acgccgtcaa tctcaccctc gtagccttcg ccgactacct
     3541 gccgaacgca ggaggtctgg gcgtcagcta cgccgtgctc gtgatcgcca tagccgcggg
     3601 ggagatcgcg gtaggtctcg cgatcgtact agccgtgttc cgctcccggc ggaccgtgaa
     3661 cgtcgatgaa gtaacctcga tgaggggcta gaagggaacc cgtgcagaca gtaggccaac
     3721 aattgatcat cacggccata ccaacgctcc ccgcgctggt cttcctggtg ctcgtcctca
     3781 caggacgcct cctcaaagag aacgcccagt acgtcgccat actcggggtg accatttcgc
     3841 tggtcttctc ggtcttcgcc ctgttgttcg tggccggtat cgtccccggc ggcggggcgc
     3901 ccatagagtt ttcggtgaac tggatagatc tcggccaggg cggctcgttc ccgatgggcg
     3961 tgtatatcga cgggctcgcc gcggtgatgc tcgtcgtcgt gtgcttcgtg agccttatga
     4021 tccagctcta ctcgggcgct tttatggagg gcgacaaacg cttcgcctgg tactacgcgc
     4081 tgctcaatct gttcacagcc tcgatgctcg ggctcgtgct cgcgccgaac ttcatccagc
     4141 tctacgtctt ctgggagctc gtcggcctgt gctcctacct gctcgtcgga cactggttcg
     4201 agaagccttc tgcccgcgac gccgcgataa aggcattcgt cgtcacgcgg atcggggacg
     4261 ctgggctgtt cgtcgggatt atcatcttct gggctaccac aggctcgacg gccttcacgg
     4321 acatctcgga ggctgcccag gccggattta tcggcggctc cctgttcacc acggccgtca
     4381 tactcgtctt catcggcgcc gttggcaaga gcgcgcagtt cccgttgcac gtgtggctcc
     4441 ccgacgccat ggagggacct actcccgtga gcgccctgat ccacgccgcc accatggtgg
     4501 cggctggcgt ctacctggtg gcccgcacct acgacatctt cgtgcagagc cccacggcca
     4561 tgctcgtcgt ggcctatatc ggcggtttta cggcgctgat ggccgcgaca atggcgctgg
     4621 tcaaaaagga cataaagcgt gtgatcgcct actctaccgt ctcgcagcta ggctatatga
     4681 tgctcggcct cgggctcggt tcctacacgg caggcgtctt ccacctctac aaccacgcct
     4741 tcttcaaggc tctcctgttc ctgtgcgccg gcagcatcat ctacgcgatg gactcttaca
     4801 acct
//
LOCUS       DATMJA010000148         4641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_374252, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000148 DATMJA010000000
VERSION     DATMJA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374252"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VNT12_03440"
     CDS             complement(<1..547)
                     /locus_tag="VNT12_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWI45201.1"
                     /translation="MAILVDEGTRLLVQGITGREGAFHTGRMLQSGTRVVAGVTPGKG
                     GQDYEGVPVFDTVEKAVEETGANASIIFVPPPFASDAIFESLDAGLSTICCITEGIPV
                     HDMMRVASYLGTKDATLVGPNCPGVLSPEKANVSIMPKDIFMPGNVGVVSRSGTLTYQ
                     IVNELTQREIGQSTAVGIGGDP"
     gene            complement(552..1703)
                     /gene="sucC"
                     /locus_tag="VNT12_03445"
     CDS             complement(552..1703)
                     /gene="sucC"
                     /locus_tag="VNT12_03445"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWI45202.1"
                     /translation="MDLYEYQGKELLREYGLETLPGIVAGTPEEAGRAAETLGGTVAI
                     KAQVLTGGRGKAGGIKVVGSPREAEEAARQILGMEILGHTVGRVYVEHGVEIDREFYL
                     SITVDRTAKRPLILFSTEGGVEIEEVAETNPEAIVRLHVNPLLGLLPYQVREITFAAG
                     LEGDVAKGVGMVLSHLYKAFEGLDANLVEINPLVVTGEGRVLTLDAKVTVDDSSLYRH
                     EDIAGLHDVEAADPLEERAQEVGLQYVKLDGDIGILGNGAGLVMSTLDVVAQAGGDPA
                     NFCDVGGGADAEQISTALDIVTSNEQVRSVLFNIFGGITRGDEVARGLLSAIERTGLT
                     LPIVVRLDGTNAEEGRWILAENTPENVVTEETMLEAARRAVELSRDGGR"
     gene            2056..2733
                     /locus_tag="VNT12_03450"
     CDS             2056..2733
                     /locus_tag="VNT12_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI45203.1"
                     /translation="MTPGTRILLVEDDRSIAGFVEPELERAGFLVRCAYDGVTGLEEA
                     RKFGPALLVLDIMLPGLDGVGILKRLREEGNGVPVIMLTARDTTLDKVHSLDRGADDY
                     LTKPFDIEELLARIRALLRRAEGDEILRVADLEINTSTREVRREEREIELTTREYELL
                     EYMAQNPRRVLSRDLLLSRVWDEELGLTTNVVDVYVGYLRRKVDAPGEEKLIRTVRGA
                     GYALKGV"
     gene            2738..4180
                     /locus_tag="VNT12_03455"
     CDS             2738..4180
                     /locus_tag="VNT12_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI45204.1"
                     /translation="MPIRWRLTLFITLVIGAILLVLGVALFILLSEALLSTVEDTAES
                     RAEDAQDIIRSGQTLHADDVKELALDGVFVIIRDGDGVVLQTYPKLTSESRAEDRVWK
                     KALDRGMPTGGPVEFSGDGMDYIYAVPVRAPQGRARVVEAGKSYKSAQEDIREIGAIL
                     AAGIGVALLLSIGGAYLLARTALRPVEAVTVTAREMGEGDIAKRLPVANPKDEIGHLT
                     ITINGLLARLDAAFRRREETLSRQRRFAADASHELRTPLTSISGHARMLDEWALEGDK
                     VTARRSVGTIRREAGKMRGLVESLLTLTRGDEGAPMEVGRYDLGAVGKEATETARDAV
                     DGKVSVDFVPIEHEVTATFDRARVMQVASILLDNAIKYTPDGGNITVSVGEEDGSVAL
                     VVSDTGIGISQDQLPLVFERFYRADSARAEEGVGLGLSIARQIAEAHGGTIKARSKLG
                     VGSTFMLLLPRQRPGPPQEGPRTQEAEDPR"
     gene            4264..>4641
                     /locus_tag="VNT12_03460"
     CDS             4264..>4641
                     /locus_tag="VNT12_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HWI45205.1"
                     /translation="MGIGTAINVLAVLAGGSIGTLAGARLPEGMRSTAMQSIGIVTLL
                     VGVSNFLKVDNVLVPLLSVILGLVVGELLDLDARLRRLGNYLERRFSKGESPVSRAFV
                     TTSLLFCVGPLTVIGSLQDGLNGD"
BASE COUNT          844 a         1450 c         1470 g          877 t
ORIGIN      
        1 tggggtcgcc gccgatcccg acggcggtgc tctgtccgat ctcccgctgc gtgagctcgt
       61 tgacgatctg gtacgtcagc gtgccgctgc ggcttacgac gccgacgttg ccaggcataa
      121 agatgtcctt cggcattatg gagacgtttg ccttctccgg cgagaggacg ccggggcagt
      181 tcggcccgac gagtgtcgcg tccttggtcc ccaggtagct tgcgacccgc atcatgtcgt
      241 gcacggggat gccttctgta atgcagcaga tggtcgagag tccggcgtcc aaagactcga
      301 agatggcgtc ggaggcgaag ggtgggggga cgaagattat gctggcgttc gcgcccgtct
      361 cttcgacggc cttctcgacg gtgtcgaaga cagggacgcc ttcgtagtcc tgcccgccct
      421 tgcccggcgt cacccctgcg actaccctgg tgccgctctg tagcatccta cctgtgtgga
      481 acgcgccctc cctgcccgtt atgccctgaa cgaggagccg cgtcccttcg tctacgagta
      541 tcgccatgaa gttatcttcc cccgtctcta gagagctcta ccgccctgcg ggccgcttcc
      601 agcatcgtct cctctgtgac cacgttctcc ggtgtgttct cagcgaggat ccagcgtccc
      661 tcttcggcgt tcgtgccgtc gagcctgacg acgatgggga gcgtgagtcc tgtgcgttct
      721 atagccgaca gcaacccccg cgccacctcg tcgccgcgtg ttatgccgcc gaagatgttg
      781 aagagcacgc tcctgacctg ctcgtttgag gtaacgatat cgagggcggt cgagatctgc
      841 tcagcgtcgg ccccgccgcc gacgtcgcag aagttggcag gatcgccacc ggcctgggcg
      901 acgacgtcaa gcgtgctcat caccagcccc gccccgttgc cgaggatgcc gatgtcgccg
      961 tcgagcttga cgtactgcag cccgacctcc tgcgcccgtt cctccagcgg gtctgcggcc
     1021 tcgacgtcgt gcagcccggc tatgtcttca tgcctgtaaa ggctggagtc gtccaccgtt
     1081 accttcgcgt ccaacgtcaa gacgcgcccc tccccggtaa cgacgagagg gttgatctcg
     1141 accaggttcg cgtcgagtcc ctcgaaggct ttgtacaggt gcgagagcac catgcctacg
     1201 cccttcgcga cgtctccttc cagccccgcg gcgaaggtga tctcacgcac ctggtaggga
     1261 agaaggccga gtagtgggtt tacgtggagc ctgacgattg cctcggggtt cgtctctgcg
     1321 acctcctcga tctccactcc gccctccgtg gagaatagga tcaggggccg tttcgccgtc
     1381 ctgtccaccg tgatggagag gtagaattcc ctgtctatct ccactccgtg ctcgacgtag
     1441 acgcgcccca cggtgtgccc gaggatctcc atgccgagga tctgccgcgc cgcctcttcg
     1501 gcctcccgcg ggctgccgac caccttgatg ccgcccgcct tgccgcgccc tccggtaagg
     1561 acctgggctt tgatcgccac tgttcccccc agtgtctcgg cggcccttcc ggcctcctcc
     1621 ggcgtccccg cgacgatgcc aggcaacgtc tccaggccgt attccctcaa tagctctttc
     1681 ccttgatact cgtagagatc caagttagaa aactcccgcg aagccggcca tgacggaccg
     1741 aaattataca gatttataag ccccaatcat gatcacaaga acctcctcac cgtacgagcc
     1801 tcgatgcggg accgttcggc cttgcacgtt ctcatctccc tctcagagag ggtctctaga
     1861 gttggccctc gtgcacccgc agttagccgc cggcggacga gaacagcctg ggtgtataaa
     1921 cggaggggca ccgtggcgcg aggtgcgcag acccgcagct ttatgtgcgc tggaagagtc
     1981 tcgctaccct tcgaactctc gcacagtcgg gacaaccaga agtacaatac tgatctagag
     2041 gccaaagagg agtagatgac accaggcacc aggatactgt tggtggagga cgaccgttcg
     2101 atcgccggct tcgtcgaacc cgagctcgaa cgcgccggct tcctggtgcg gtgcgcctac
     2161 gacggtgtca ctggcctgga ggaggcgcgc aaattcgggc ctgcgttgct cgtcctggat
     2221 atcatgctgc cggggttgga cggggtcggg atcctcaagc ggttgcgcga ggagggcaat
     2281 ggggtgcctg tgatcatgct gacagcccgc gacaccacgc tcgacaaggt acacagcctc
     2341 gaccgcggtg cggacgatta cctgaccaag ccgttcgaca tagaggaact acttgcccga
     2401 atccgcgcgc tgctgaggag ggctgagggc gacgagatac tccgtgtggc cgacctggag
     2461 atcaacacct ccacccgcga ggtgaggcgg gaggagaggg agatagagct aacgacacgc
     2521 gagtatgagt tgctcgaata tatggcccag aaccctcgcc gggtgctctc ccgcgatctc
     2581 ctgctctcgc gcgtctggga cgaggagtta ggccttacca ctaacgtggt ggatgtctac
     2641 gtcggctacc tcaggaggaa ggtggacgcc cctggcgagg agaagctgat ccgcactgtg
     2701 cgtggggcgg gctacgcctt gaagggggtg tgagcacttg cccatcaggt ggcgcctgac
     2761 gctgttcatc accctcgtga taggggcgat actcctggtc cttggcgtgg cgctcttcat
     2821 cttgctcagc gaggccctgc tctccaccgt cgaagacacc gccgaaagcc gggccgagga
     2881 cgcccaagat atcataaggt cggggcagac tctgcacgcg gatgacgtca aggagctcgc
     2941 cctcgacggg gtcttcgtga tcatccgtga cggagatgga gttgtgctcc aaacataccc
     3001 taaactcact tcagagagta gggctgagga ccgtgtctgg aagaaggcgc tcgaccgagg
     3061 catgccgacc ggtggcccgg tcgagttctc cggagacgga atggattaca tctacgccgt
     3121 gcctgtgcga gcaccgcaag gacgcgcaag ggtggtggag gcgggaaagt cctacaagtc
     3181 ggcgcaggag gatatccggg agatcggtgc gatcctcgcc gcaggcatag gcgtggccct
     3241 cctcctctcc ataggcggcg cctacctgct tgcacggacg gccctgcggc ctgtcgaagc
     3301 ggtaacggtt acggcgcggg agatggggga gggggatatc gcgaagcggc tgcccgttgc
     3361 gaatccgaaa gatgagatcg gacacctcac catcacgata aatggcctgt tggcgcgtct
     3421 cgatgccgcc ttcaggcgca gggaggagac cctttcgcgc cagcgacgct tcgccgccga
     3481 cgcgagccac gagctgagaa cccccctgac ctcgataagc gggcacgcca ggatgctcga
     3541 tgagtgggcc ctcgagggag acaaagtgac ggcgcgtcgg agcgtcggca ccatccgcag
     3601 ggaggcgggg aagatgcgcg gcctggtcga gtccctacta acgctaaccc gcggggatga
     3661 gggcgcaccc atggaggtag ggcgttacga tctcggtgcg gttgggaagg aggctaccga
     3721 gactgccagg gacgccgttg acggcaaagt gtcggtcgac tttgttccca tcgagcacga
     3781 agttacagcc acctttgacc gagcgcgcgt aatgcaggta gcttccatcc tcctagacaa
     3841 cgcgatcaag tacacgcctg acggcgggaa catcacggtc agtgtggggg aggaagacgg
     3901 cagcgttgcg ctggtggtct ctgatacggg gattgggatc tcccaggacc agttgcccct
     3961 cgtcttcgag cgcttctaca gggcggactc tgcgcgggcc gaggagggag taggccttgg
     4021 actctctatc gcccgccaga tcgccgaggc ccacgggggc accataaaag ccagaagcaa
     4081 gctaggcgtg ggctctacgt tcatgctcct gctcccccgc cagaggccag gaccgcccca
     4141 agaaggcccc cgtacacaag aggccgaaga cccgagataa cgggctgcag gtacccggcg
     4201 gtcgacccga acctacatca ccacgggtac cagtaatcct catgtcatat actgctgact
     4261 cccgtgggaa ttggaaccgc gatcaacgtt ctcgccgtgc tcgccggcgg gagtatcggc
     4321 actctcgcag gcgccaggct acccgagggc atgcgctcga ccgcgatgca gtctatcggt
     4381 atcgtaacgt tgctcgtcgg cgtctcgaac ttcctgaaag tcgacaatgt gctcgtgccg
     4441 ctactcagcg tgatcctggg tctcgtggtc ggggagctcc tggatttaga cgcacggctc
     4501 agacggctag ggaattatct ggagaggagg ttctcgaagg gcgagagtcc ggtaagccgg
     4561 gctttcgtga caacgagcct cctgttctgc gttgggccgc tgacggtgat cggctcgctc
     4621 caggacggct tgaacggaga c
//
LOCUS       DATMJA010000149         2893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_551959, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000149 DATMJA010000000
VERSION     DATMJA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551959"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1068
                     /locus_tag="VNT12_03465"
     CDS             169..1068
                     /locus_tag="VNT12_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45206.1"
                     /translation="MMSKTNDNTGYKRYLSQRYLASLPERTARVGAALTGGLVYETGE
                     VLLPLAVRRSRLYQAIVGRLLRITIELVGGVEGVYPTEELRVRELLVRKTAGNAIELS
                     SFLAVGWSPVWLLAGASDLIGGTKVYLRVLATELRDTGVLSAEEDVASFEELLTALEG
                     TSGVLADTVDVPPLNVSSVRTSWQELRRQVADLPDADGLEKIFSDLQLAAQQEDRSTL
                     EISSIVALGAVHAGVRLGDAHIFDYYRGALRTIAVEGLVAFLHRASTPYLTRARSHFD
                     PGSSTYSERLLRRWADRRSPVAR"
     gene            complement(1455..2678)
                     /locus_tag="VNT12_03470"
     CDS             complement(1455..2678)
                     /locus_tag="VNT12_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HWI45207.1"
                     /translation="MKQPRRFETRAVHSGEHRAGAKEGRDGIFYPISTPIYASTTFSH
                     PNIETTDRVLGGEEQGYSYARYDNPTVRAFEEALVSLETSESEAGVRGFAFASGMAAT
                     HAALAAAELTGGGTVLAADQLYGSTATLLVQVFGASGVETRFVDAYDLGAVEKKVAEL
                     KPRAVVVESISNPLLRVADIPQISKLTRAAGVALIVDNTFGTPYLQRPLELGADIVVH
                     SATKYLSGHGDLTAGAVVAGPPYAAAVEQVRKLVGGVLGPFEAWLAHRGLKTLPLRMD
                     RQCKNARQIASNLVAHPKVAKVNHPSLGAHPDHQTAGRVLSDTGGLVSFELSVEGREA
                     AYAFLNALELCVKAPSLGDIYTLAIHPATSSHRELSPSRRERLGVGENLVRLSVGIEH
                     AGDVISDLEQALERI"
BASE COUNT          544 a          882 c          929 g          538 t
ORIGIN      
        1 aaggcgaaga accaccaggc gccctccagc cccgtgccct acctcatggg tgtaagatac
       61 attcgtgtca tgaggtggta ggacagttgg aatatatgaa ggtttatcca cgcgagtgcc
      121 caaaaccatg gaagataggg cacccaacgc agcaccagga ggcgggcgat gatgagcaag
      181 acgaacgata atacaggata caaacgttac ctttcacaaa ggtatctggc atccctgccc
      241 gaacgaaccg cgcgagtggg agccgcgctt acaggcgggt tggtatacga gacgggtgag
      301 gtgctactac cgcttgcggt tcgccgatcc aggctctacc aggcgatagt cggacggttg
      361 ctgcgtatca cgatcgagtt agtaggcggc gtcgagggcg tctaccccac cgaagagctg
      421 cgggtacgag agctgctggt acgcaagacg gcgggtaatg ccatcgagct tagcagcttt
      481 ctggcggttg gttggtcacc ggtctggctc ctggcagggg cttctgatct gatcgggggt
      541 acgaaggtat atctgcgggt gctagccacg gagttacggg ataccggcgt actttcggcg
      601 gaagaagacg tggcatcatt cgaggagctg ttgactgcac tagagggcac ctccggtgtg
      661 ctagccgaca ccgttgacgt gcctccactg aacgtctcca gcgtgcgcac ctcgtggcaa
      721 gaactacgtc ggcaggtagc ggatttgccg gacgccgacg gtctagagaa gatcttctcc
      781 gacctacagt tggccgctca gcaagaggat cgctcgacat tggagatttc ttccattgtt
      841 gcgctcggtg cggtgcatgc gggcgtgagg ctaggagatg ctcacatatt cgactactat
      901 cgcggcgcgc tgcgaaccat cgcagtcgag ggtctggtcg ccttcctgca ccgagccagc
      961 accccctacc taacgcgggc gcgttcgcac tttgatcctg ggtcgagtac ttactcagaa
     1021 cgccttctac ggcgttgggc agaccgccgt tcacctgtgg cacgctaaat gacgacgcag
     1081 ggtgcggcct cgagcaagga aaacgccgct gaggaccatc cgatgggtga gggacaagac
     1141 tcgaaaggca ggaaaccaat gtgaacgcag ctccgaggac atgaagcagg agctcccacc
     1201 ctcgctcgct ttccttccgc gcagcgcgcg acagatcgtc gtgagcgcgc cgtctcagcc
     1261 ggtcgagttt atcctcgctc gcctggaaag cgtaaagaag caaaacggct tctacgggcc
     1321 ttctgcccgc agacgacgat aggagcaccc cgaacctcga cgtcaaggag cgagcgggca
     1381 gccgtgtcct cctctgctgc gcgggctgct ccactgagta gctgggccgc gcggctcacg
     1441 atgcctcctg ctctctagat tctctcgagg gcctgttcga ggtcggagat cacatccccg
     1501 gcgtgctcga taccaaccga gagccgcacc aggttctccc cgaccccgag cctctcccgc
     1561 cgcgacggcg acagctcgcg gtgcgaggag gtggcgggat gaatggcaag ggtatagatg
     1621 tctcctagag aaggggcttt gacgcacagc tccaacgcgt tgaggaaggc gtaggcagcc
     1681 tcacgcccct cgaccgagag ttcgaaggag acgaggccgc ctgtgtccga gagcacacgc
     1741 cctgcggtct ggtgatccgg atgcgctccg agtgaaggat ggttgacctt cgccaccttc
     1801 ggatgggcga cgaggttgga tgcgatctgg cgcgcgttct tgcactggcg gtccatgcgc
     1861 aagggcaacg tcttcaggcc gcggtgcgcg agccaggcct cgaaaggacc aagcacgcca
     1921 ccgacgagct tgcgtacttg ttctaccgct gctgcgtaag gcggaccggc gaccaccgcg
     1981 ccggccgtga ggtccccgtg tcccgacaag tacttggtcg cgctgtggac gactatgtcc
     2041 gcgccgagct ccaggggccg ctggagatat ggtgtgccga aggtgttgtc cacgatcagg
     2101 gcgacccccg cagcacgggt tagcttcgag atctgcggta tgtcagcgac gcgcaagagc
     2161 gggttggaga tagactcgac aacgacggcg cggggcttga gttcggcgac tttcttctcc
     2221 accgctccca gatcatacgc gtcgacgaag cgggtctcga cgccgctcgc gccgaagacc
     2281 tggacgagca gcgtcgccgt cgatccgtag agttggtcgg cggctaggac cgtgcccccg
     2341 ccggtcagct ccgcagccgc gagcgcggcg tgggtagcag ccatccccga ggcgaaggcg
     2401 aaacccctga ccccagcttc ggactcggag gtctccagcg acacgagcgc ctcctcgaag
     2461 gcccggacgg tagggttgtc gtagcgggcg tagctgtagc cctgttcctc tccgccgagc
     2521 acgcggtccg tggtctctat gttcgggtgg gagaaggtgg tcgaagcgta gatcggcgtc
     2581 gagatggggt agaagattcc atcccggcct tccttcgcac cggccctgtg ctctccggag
     2641 tgcacggcgc gggtctcgaa acgtctcggc tgcttcatgg tggcttatac tacgacctgg
     2701 ttgccccggt ggcgaccgtc gccccgaagt cgaagcaacc cgttgttccc ggcgcctaca
     2761 acgacggcgt cgtactgcgt cgacatagct cgacctcccg agcatagcct tgacctcatt
     2821 cgccgtcggg cgtgtcccta ggtccatcgt ctcctccttg gggctctgta tctcctctca
     2881 tgacacgcca ggc
//
LOCUS       DATMJA010000150         3615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_255785, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000150 DATMJA010000000
VERSION     DATMJA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255785"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VNT12_03475"
     CDS             <1..485
                     /locus_tag="VNT12_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HWI45208.1"
                     /translation="ITERRIDRMIDPRRSEGLPAFLSPRPGVNSGFMIPHYTAAALAE
                     ENRRLANPASTGSLPTSAMQEDHNSMGWSAGRELRRLLGNVCRILAIETACAAQALDL
                     RSPLRPGHATSAVLERIRQDIPFVEGDTFMAPHLEAAERLVSSGALVGAAERAIGPLA
                     "
     gene            691..2385
                     /locus_tag="VNT12_03480"
     CDS             691..2385
                     /locus_tag="VNT12_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI45209.1"
                     /translation="MVALLSLLLVSGANLVTPLLIGRAIDEGIGPRRLGVIFVMVGWL
                     VGLALARALFTFLQGYLAERASQGVAYDLRDALFERIERLSFSYYDRVQTGQLVTRLT
                     SDVEQIRTFAGSGVVQLANAVVMLIGTTAVLLYLDWQLALVALAIVPIIAVLLVRFVG
                     RIRPLFREVQQTLGMLNTVLQEDLLGVRVIRAFAREDYETARYTSVNDELLEKNLTTV
                     RVFSNNFPFVFLFANLGTLAIIWFGGWQVIGGRLSVGDLVAFNTLLGFMLFPILTIGF
                     LSASISRAGASSQRVFDVLDAPLDVKDAPDASTLLPLSCRVDFDNVSFRYPGSARDIL
                     AGVSFTVKPGQTVAVLGTTGSGKSTLVNLIPRFYDVTGGAVRLDGNDVRDVTLSSLRS
                     QIGIVLQETRLFSGTVRDNVAFGKPDATDEEIVAAAEAAQAGEFIRGLSEGYETVIGE
                     RGIGLSGGQRQRIAIARALLIDPRLLILDDSTSAVDAETEAAIQETMDRLMREKHRTV
                     FVIAQRVSTVRDADLILVLDEGSIAASGTHEELLRESELYNEILGSQLLSPSETAA"
     gene            2491..3039
                     /locus_tag="VNT12_03485"
     CDS             2491..3039
                     /locus_tag="VNT12_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWI45210.1"
                     /translation="MSETTEERTARDRVSFLRTLRAVRSFRSDPVPREVLDDVLEVAR
                     WSGSASNKQPWEIVVIRDRDTLRSLASVGGYAGHLAGAPLGIVLVMAGERAEQETYDE
                     GRVAERIMLAAHAHGVGSSIGWIVGPGRDAARELLRIPKEKMVRTAISLGYPDEGARR
                     SRAGRGRARKSLGEIVHTESYA"
     gene            3218..>3615
                     /locus_tag="VNT12_03490"
     CDS             3218..>3615
                     /locus_tag="VNT12_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWI45211.1"
                     /translation="MPQERAGDGGATARRLLGELRPYSGKLVLVLGLVVLGAAAQAGG
                     PWLIGRAIDRYILRGDVAGLSRTMLVLFGVYSVGAIASRGQVYQVGSIGQSVLASMRA
                     RIFDRLQHLPLRFFDRRPVGDLMSRVTNDV"
BASE COUNT          657 a         1170 c         1168 g          620 t
ORIGIN      
        1 caattacgga gcgcaggatc gaccgcatga tcgacccgag acgctcggaa ggcctgccag
       61 cctttctaag cccgcgaccc ggcgtcaact cggggttcat gatcccccac tacacagcag
      121 cggccctcgc ggaagagaat cgccgcctcg cgaacccggc gagcacaggg tcccttccga
      181 cctccgccat gcaagaagac cacaactcga tgggctggag cgcgggccgc gaacttcgcc
      241 ggctcctcgg gaacgtctgc aggatcctcg ccatcgaaac ggcgtgcgcg gcccaggccc
      301 tcgacctccg ctcccccctg cgaccaggcc acgcaaccag cgccgtcctc gagcgcatca
      361 ggcaagacat cccgttcgtc gagggcgaca cctttatggc cccgcacctc gaagcagcag
      421 aacgtctggt gagttcagga gcgctggtag gagcggccga acgagccata ggccccctcg
      481 cctagagagc ggtcgacagg gaggccgatg ggataccata agagcggatg ccaacagaga
      541 accatcaagg cagagtcgcc catggctgcg gtgagccggc gaccgtgatg ggcgcgtaga
      601 ggacctcttc tggccggcag ggagacacca tcgggagcgg gggcgctccg cagggcgctc
      661 gcgtacctcc gctcgtatac gtggcagagc atggtcgcgc ttctctcgct gctgctggtc
      721 tccggggcca acctggtgac accactactc atcgggcgcg ccatcgacga aggcatcggt
      781 ccgcggcggc tcggggtcat cttcgttatg gtcgggtggc tggtcgggct cgcgctggca
      841 cgggccctgt tcacgttctt gcaggggtat ctggccgaga gggcttcaca gggtgtcgcc
      901 tacgacctgc gggacgccct gttcgagagg atagaacgac tctccttctc ctactacgac
      961 agggtccaga caggccagct cgtcacccgc ttgaccagcg acgtcgagca gatccgtacc
     1021 ttcgccggct ccggtgtcgt ccaactcgcc aacgccgttg tcatgctcat aggcacgaca
     1081 gcagtgctcc tctatctcga ctggcaactc gccctcgtcg cgctggccat agtccccatc
     1141 atcgccgtat tgttggttcg ctttgtcggt aggatcaggc cacttttcag ggaggtccag
     1201 cagacgctcg gaatgctcaa caccgtgttg caagaggacc ttctgggggt gcgagtgatc
     1261 cgggccttcg cccgcgagga ctacgagacg gcccgctaca catcggtcaa cgacgagctg
     1321 ctggagaaga acctcaccac cgtgcgggtg ttctccaaca acttcccatt cgtgtttctg
     1381 ttcgccaacc tggggacact cgcaatcatc tggtttggcg gttggcaggt tataggcggc
     1441 agactctcgg tcggggacct cgtcgctttc aacaccctgc tcggctttat gctcttcccg
     1501 atcctcacca taggcttcct ctcggcgagc atctcgcgcg cgggagcctc atcccagagg
     1561 gtcttcgatg tcctcgacgc accgctcgac gtcaaggacg cccccgacgc ctccaccctc
     1621 ctcccactct cctgcagggt cgacttcgac aacgtctcgt ttcggtaccc tggcagcgcg
     1681 cgcgacattc tggccggcgt gtcctttacc gtcaagccgg ggcagaccgt ggccgtcctg
     1741 gggaccacgg gctcggggaa gtccacgctc gtcaacctca taccacgctt ctacgacgtg
     1801 acgggagggg ccgtcaggct cgacggcaac gacgtgcgtg acgtgaccct atcgtcgctc
     1861 aggtcccaga tcgggatagt cctgcaggag acgcgcctct tctccggcac cgtgcgcgat
     1921 aacgtggcct tcggcaagcc ggatgcgacg gacgaggaga tcgtggcagc ggcagaggcg
     1981 gcccaggccg gtgagttcat aagggggctc tcggaaggct acgagaccgt tatcggcgag
     2041 cggggcatcg ggctctccgg cgggcagcgg cagcgaatag ccattgcgcg ggcgcttctt
     2101 atcgacccgc ggctccttat tctggacgac agcacctcgg ccgtggacgc tgagacagag
     2161 gctgctatcc aggagactat ggacaggctc atgcgcgaaa agcatcgcac ggtcttcgtc
     2221 atcgcccaga gggtctctac ggtgcgggac gccgatctga tcctggtcct cgacgaaggc
     2281 agtatcgcgg ccagcggcac ccacgaggag ctcctgcgcg agagcgaact ctacaacgag
     2341 attctcggct cccagttgct ctccccctcg gagacagcag catgaccgga gggctcggta
     2401 cagtacacct tgggccagct tcattcaaac ggaatgagcg gacgtgaaga aaaggacaat
     2461 tggcgtgggt gctgctgaga ggagagaacc atgagcgaga ccacagaaga aaggaccgca
     2521 agagaccggg tctcatttct gcgcaccctg cgcgccgtcc gctcgttcag atccgaccct
     2581 gtaccgcggg aggtcttgga cgacgtcctg gaggtggcac gctggtcagg gagcgcgtcc
     2641 aacaaacagc cctgggagat cgtggtgatc cgggatcggg acacgctgcg ctccctcgcc
     2701 tcggtcgggg gctacgcagg gcacctggcc ggggcgccgc tcgggatagt cctcgtcatg
     2761 gccggcgagc gggccgagca ggagacctac gacgagggga gggtcgccga gcggatcatg
     2821 ctcgccgccc acgcgcacgg ggttggctcg agcataggtt ggatcgtcgg ccccggcagg
     2881 gacgcggcca gggagctact gcgtatacca aaggagaaga tggtgcggac tgcgatctct
     2941 ctcggctacc ccgacgaggg ggcccgcaga tcccgtgccg gccgcgggcg ggcgcgtaaa
     3001 tccctcggcg agatcgtcca cacagaaagc tacgcttagg agaatcatga acgattcttt
     3061 cgacgccgaa accctcaggc tcctcgacga gaccaaggaa gtgcgcatcg agaccctgcc
     3121 cacgaccatc aagctctcgc cagcctagag gctggttcgt acaccatata gagtcgtccg
     3181 aggaggagag atcaggggca ttgaatccat cgcacgcgtg ccgcaggagc gtgccggcga
     3241 cgggggcgcc accgccaggc gcctcctggg agagctgcgt ccctattcgg gcaagctggt
     3301 gctcgtgctc gggctcgtcg tgctcggggc ggcggcgcag gctggagggc cctggttgat
     3361 cggacgcgcc atagaccgct acatcctgcg cggcgacgtg gccgggctct ccaggacgat
     3421 gctggtgctc ttcggggtct actccgtggg ggccatagcc tcgcggggcc aggtatacca
     3481 ggtcggttcc atagggcaga gtgttttggc ctccatgcgg gccaggatct tcgaccggct
     3541 ccagcacctc ccactgaggt tcttcgacag gaggccagtc ggagacctga tgagtcgggt
     3601 aaccaacgac gtcga
//
LOCUS       DATMJA010000151         3220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_552547, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000151 DATMJA010000000
VERSION     DATMJA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552547"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..164
                     /locus_tag="VNT12_03495"
     CDS             6..164
                     /locus_tag="VNT12_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HWI45212.1"
                     /translation="MLARKPTEVLRKAVYGMMPKTRLARKQFKKLKIYAGPEHPHSAQ
                     NPQTLEVR"
     gene            165..557
                     /gene="rpsI"
                     /locus_tag="VNT12_03500"
     CDS             165..557
                     /gene="rpsI"
                     /locus_tag="VNT12_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWI45213.1"
                     /translation="MAEALYSGTGRRKTAVARVRVLPGDGKITVNGRDVNEYFPRPAY
                     RTAVRSPLELLGSAGRYEVIAKIEGGGPTGQAEALRHGIARALAEESQEARSELKAAG
                     MLTRDSRAVERKKYGLKKSRKRPQFSKR"
     gene            644..766
                     /locus_tag="VNT12_03505"
     CDS             644..766
                     /locus_tag="VNT12_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45214.1"
                     /translation="MSPDEHIEYAPFEAEAFYETFLHKKGQGFRIYPGMAREAD"
     gene            778..2091
                     /gene="glmM"
                     /locus_tag="VNT12_03510"
     CDS             778..2091
                     /gene="glmM"
                     /locus_tag="VNT12_03510"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWI45215.1"
                     /translation="MSFGTDGVRGVANKGLLPEDALRLGLAASRVFGGRILVGRDTRV
                     SGEMLASALCAGAASGGAGVVDLGVLPTPGVAALAPRLGATAAGVVSASHNPYPDNGI
                     KFFSGDGRKLSPEKEREIEDLLGSDFTRPTGSGIGSVEVSGDATRIYAEWVLQQLRPS
                     AEGMEVLLDCANGAAYEVAPLVFGEIGVDLTLTGADPTGTNINEGCGSTHVEELDVSG
                     QDVAFAFDGDADRVLAVDERGNVVDGDRIIAILARDKRERGVLSGEVVVTVMSNLGFF
                     KAMEAMGIPYEVTDVGDRHVAEAMNRLGASLGGEQSGHVILSGHTTTGDGIVTALALL
                     DVMVRSGRPLSELAAAMEVYPQTLINVRVEELGAAKAVAASEAVRLAVSEAESTLGEE
                     GRVLLRPSGTEPVVRVMVEHTDETVCREVCEEISRSVYDVGKEGT"
     gene            2088..2945
                     /locus_tag="VNT12_03515"
     CDS             2088..2945
                     /locus_tag="VNT12_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45216.1"
                     /translation="MRFVGCALAWRPGEAREKVSCLVVLDERGSIIANSFVAGAEEIA
                     RTVEGYGSERRGTVVGVDAPLAVPNERGTRKIERILSKVALPAYSASRRMFGGEPYAE
                     ELLSELEKVGVEYTDYPFPRERGQSVVVEVDSAATLKVLTLERSGAEDNGDLAKRLRA
                     MDDPKLRKGNKESRAAALKGAIEVLWDTPGLRLRTGNLSADISASENVDVSKLDVSAS
                     MSHAELDRIVVLVEGTLAAYTVHRHWRGRDGSIVVGAGDEGSVLLPARNALRERLAEE
                     CGNAGVPYV"
     gene            3099..>3220
                     /locus_tag="VNT12_03520"
     CDS             3099..>3220
                     /locus_tag="VNT12_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWI45217.1"
                     /translation="MSLKIRIIPCLDVDAGRVVKGTKFTNLRDAGDPVELAALY"
BASE COUNT          627 a          928 c         1133 g          532 t
ORIGIN      
        1 agcagatgct cgctcgcaag ccgaccgagg tattgcgcaa ggccgtctac gggatgatgc
       61 cgaagactcg cctcgccaga aagcagttca agaagttgaa gatctacgcg ggacccgagc
      121 acccacactc agctcagaac ccgcagacgc tcgaggtgag gtaaatggca gaggctctat
      181 atagcgggac gggaaggcgc aagaccgccg ttgcacgggt gagggtactc cctggcgacg
      241 gtaagatcac cgtgaacggc cgcgacgtga acgagtactt cccgcgtccg gcttatcgca
      301 cggcggtgcg ctcgccgctc gagctccttg gttcggccgg ccgctacgag gtcatagcca
      361 agatcgaagg cggcggtcca acaggacagg cagaggcctt gcgccacggt atagcgcggg
      421 cgctcgccga agagtcgcag gaggccagga gcgagctgaa ggccgcaggg atgctcaccc
      481 gtgattccag ggcggtggag cgcaagaagt atggcctcaa gaagtcccgc aagcgtccgc
      541 agttctccaa gcgttagagc cgcaagagcg gatcttcatg tcgggctcct ttcgcgcggc
      601 ggaaggggcc cgttctacgt agagacgggg agaaagaacc ggtatgagtc cggatgagca
      661 catcgagtac gcgcccttcg aagccgaagc gttctacgag actttcttgc acaagaaggg
      721 ccagggtttc aggatctatc ctggcatggc aagggaggcc gactgaagag agagcccata
      781 tcgttcggca ccgacggagt acgcggggtc gccaacaagg gcctgctgcc cgaggacgcc
      841 ctgcgccttg gcctcgccgc ctcccgggtg ttcggaggca ggatcctcgt cggacgcgac
      901 acgcgcgtct ctggagagat gctcgccagc gccctgtgtg ccggcgctgc gagcggcgga
      961 gccggcgtcg tagatctcgg tgtccttcct acgcccggcg tcgcggctct cgcgccgcgt
     1021 ctcggggcca cggccgcagg cgtcgtaagc gcctcgcaca atccctaccc tgataacggc
     1081 atcaagttct tctcaggaga tggccgcaag ctctcccctg agaaagagcg tgagatcgag
     1141 gacctgctgg gcagcgactt tacgcgcccg accgggagcg gtatcggaag tgtggaggtg
     1201 tcaggcgacg ccacacgtat atacgcggag tgggtgctcc agcagttgcg tccgtcggct
     1261 gagggtatgg aggtgctcct cgactgcgcc aacggggcgg cctacgaggt agcgcccctg
     1321 gtgtttgggg agatcggagt ggatctcacc ctgaccggag ccgatcccac gggtacgaac
     1381 ataaacgagg gctgcggctc gacgcacgtc gaggagctcg acgtctcagg acaggatgtg
     1441 gctttcgcct tcgacgggga cgcagacagg gtgctcgcag tcgatgagcg ggggaacgtc
     1501 gttgacgggg ataggatcat cgccatcctc gcccgcgaca aaagggaacg cggagtcctc
     1561 tccggcgaag tcgtggtcac cgtcatgagc aacctcggct tcttcaaggc catggaggcc
     1621 atggggatac cttacgaggt gactgacgtg ggtgacaggc acgtcgccga ggctatgaac
     1681 cggctcggtg cttcactggg cggggagcaa tctgggcatg tgatcctctc cgggcacacc
     1741 accacaggag atggcatcgt tacggccctc gcgctcctcg acgtgatggt tcgcagcggc
     1801 aggccgctct ccgaactggc cgcggcgatg gaggtgtatc cgcagacgct catcaacgtg
     1861 agggttgagg agcttggggc cgcgaaggcc gtcgcggcct cggaggcggt acggctagcc
     1921 gtctcagagg cggagagcac tctcggtgag gaaggacgcg tcctattgag accgagtggg
     1981 acggagccgg tcgtgcgggt tatggtcgag catactgatg agacggtgtg ccgggaggtc
     2041 tgcgaggaga tctctcgctc cgtatacgat gtgggtaagg agggaacttg aggtttgtag
     2101 gctgcgcgct ggcgtggagg ccaggcgagg ctcgggagaa ggtctcgtgc ctcgtcgtgc
     2161 tggacgagcg ggggagcatc atagccaact cgttcgttgc gggcgcagag gagatagcaa
     2221 ggacggtcga gggctacggc tcggagcggc gggggaccgt cgtcggcgtc gacgctccgc
     2281 tcgcggtccc gaacgagagg ggaacgcgaa agatcgaacg catcctctcg aaggtggcgc
     2341 ttcctgccta ctcggcgagc cgcaggatgt tcggcggcga gccttacgca gaggagttgc
     2401 tctccgagct ggagaaggtc ggcgtggagt acacggacta ccctttccca cgagagaggg
     2461 gccagagcgt ggtcgtggag gtagactccg cggcgacgtt gaaggtcctc actttggagc
     2521 gctcgggtgc ggaggacaac ggcgacctcg caaagcggct ccgggcgatg gatgacccca
     2581 agctccgcaa aggcaacaaa gagagcaggg cggcggcgct caagggcgcc atcgaggttc
     2641 tctgggacac gccgggcctc aggctaagga cgggcaacct ctccgcggac atctccgcat
     2701 cagagaatgt ggacgtctcc aaactcgatg tctccgcctc gatgtcccac gcagagctcg
     2761 acaggatagt tgttctggta gaagggacgc tcgccgccta cacggtccac cgtcactgga
     2821 ggggaaggga cggctccata gtagtaggcg cgggggatga gggctctgtc ctgctgccgg
     2881 cgaggaacgc cttgcgcgag cgcctggccg aagagtgcgg caacgccggt gtgccctacg
     2941 tttagccccg tacaataccc tgtaatgagc taccaggaag ggacagcggt gaagttcgtt
     3001 tcgttgaacg tggccctgcc acaggcgcag cgttacatac gcgccctctt cgctgagcgc
     3061 ctggctaatc ctgggcgcgg acgggagaag agcccccggt gagcctcaaa atccgcatta
     3121 taccctgcct cgacgtggac gccgggcggg tggtaaaagg tacaaaattc accaatctga
     3181 gggatgccgg agatccggta gagctcgcgg ccctctacga
//
LOCUS       DATMJA010000152        11218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_19560, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000152 DATMJA010000000
VERSION     DATMJA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19560"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VNT12_03525"
     CDS             <1..683
                     /locus_tag="VNT12_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWI45218.1"
                     /translation="DRGWGLNSQVNEPHRRAVESGYDLMAEQYLATKDPEDPLALRAL
                     EDLASLLPSEAAVLDLGCGAGVPVTRWLADRGFTVTGVDVSAKQLELARANVPDGIFL
                     KADMTEVVFGPESLDAVVAFHSIIHVPRGEHRTLLENINRWLKPGGALLATMTVTDYE
                     GHEEDWERWGAPMVWSHYDRNANVAMLKDTGFQIRYAEPRTGGGTGDETETWLWVLAS
                     KRSREGAT"
     gene            680..1990
                     /locus_tag="VNT12_03530"
     CDS             680..1990
                     /locus_tag="VNT12_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HWI45219.1"
                     /translation="MTTITKAVASDIRFPTSLDLSGSDAMNEAPDYSAAYVVLQTDGG
                     LEGHGFTFTIGRGNELCVAAIRALAPLVEGRTLESFTRDMGAFWRHVTGDSQLRWVGP
                     EKGVIHLATAAIVNAVWDLYAKAEHKPLWRLLSDMSPEELTSCIDFRYITDAITREEA
                     LEILRRNEPTKPDREAEMLEDGFPAYTTSAGWLGYSDERLRSLVREAVGKGWTHFKMK
                     VGGDIEDDVRRAALIRDEIGPDRTLMMDANQVWDVDQAIENMERLGEFEPYWIEEPTS
                     PDDVLGHARIAEAVSPFGVATGEHCQNRVLFKQLLQADAISFCQIDACRLGGVNEVIA
                     VLLMAAKFGVPVCPHAGGVGLCEYVQHLSIFDYICVGASLEDRILEYVDHLHEHFVDP
                     VEIQGGRYVPPQASGYSAEMKPESLNEFEFPEGSAWATGEKSIR"
     gene            1987..2700
                     /locus_tag="VNT12_03535"
     CDS             1987..2700
                     /locus_tag="VNT12_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI45220.1"
                     /translation="MNGRVAAVFGVGPGLGAVVARRFAGEGFAVAMMARREESLAEIR
                     QDIEDDGGTALPVSADATDADSVAVAFEGIRSDLGDPEVLVYNAGAFQMGGVLEISPA
                     QFDECFRANCAGAFYAAREVLPAMVEAGRGTILLTGASAALRGKARFSALAVGKFGLR
                     ALAQSMAREFGPQGIHVSHVIIDGQINTPSIREMMPDREEHTMLSPDAIAETYWQLHS
                     QDRTAWTLESDLRPSVESF"
     gene            complement(2703..3473)
                     /locus_tag="VNT12_03540"
     CDS             complement(2703..3473)
                     /locus_tag="VNT12_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HWI45221.1"
                     /translation="MPTEGRHMLTIHHLYADMMNLYGDRGNVISIEKRCEWRGIAVEV
                     VDVGLTEKIRPTGCDLFLFGGGQDREQALLAEDLSGSKGGDLRAIVEDGGVVLGVCGG
                     YQLMGHSYETPVGEKLPGVGIFDLHTEPRKANEERLIGNVLVRVQSPETGETREIVGF
                     ENHGGRTYLGEDLKPLGEVVVGYGNNGKDGTEGARRLNAYGTYLHGSLLPKNPWLTDQ
                     LILNALRRVDESFELEPLDDTMEGRAFSSVAGRVAGGR"
     gene            complement(3510..4922)
                     /locus_tag="VNT12_03545"
     CDS             complement(3510..4922)
                     /locus_tag="VNT12_03545"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HWI45222.1"
                     /translation="MSRLPDPRLSAAILAARSARFASRTLKTGGGSTVPGVVARRVDP
                     KVLTKLSRRLRLGSAAITGTNGKTTTTRMVSKILQIAGVRAVNNSTGANLVTGVTAAL
                     VSDSGLAGAPLSEMGLFEVDEASVPKVAAEAEIEILAVLNLFRDQLDRYGELAYTGKV
                     IASSFGDLPQSGAVVLNADDPLVASLGRSARRPAFYGVDEPSLDTGRLQHVADSKDCP
                     VCGTALVYETVYMGHVGLYDCRHCDFSRPEPAYRASRVRLQGAQGSEFLLSTPKDKAN
                     VKLGLPGLYNVYNALAAAAVAGEAGVGLAEISRGLSEFGGAFGRVERVKAGDREAFLL
                     LIKNPVGFNEILRTFVTGADAKNVLIAINDNDADGRDVSWLWDVDFEMLADARKEGKA
                     DATPFTVSGIRAEDMAVRLKYAELPVGPVIPDREEAIKSALAATPPGETLYVLPTYTA
                     MLEIRQTLSEMGYTHPFWED"
     gene            complement(5005..7161)
                     /locus_tag="VNT12_03550"
     CDS             complement(5005..7161)
                     /locus_tag="VNT12_03550"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HWI45223.1"
                     /translation="MTRLRSPDALVLLSCVAAVILLLPLRELSGVFPPLAFAATLVLF
                     TAPGLLLSHWLLRDDMSGTALVPVGFAISTGIFGLLGVPALILHLTTENYLLAAGTLL
                     AAFLAVAVWRTVRPKELTVESSPERDAPYGPPAGWLWAPFALLCGVLAFVGTRRVPSS
                     YDDIWVYLSWVKDFANSERLALVDPYFGERIAEFSRAKVNGWLLEQAALSRVSGLDTI
                     ELVLRYLTPTLVVVALLVVYVLALAISKSERAAVLIASVYALFHIIFIQPSVHNVGVE
                     LAARISEDKYAARFLILPITLLFALLFVESRRWRHLGLFTFFCWAAVAVHPSVLPAIG
                     LCMLGFGVAHVAVNLRLRAAWTGMVVLALGMWSVALGPVVLLLFAGRSPEAVLFSADI
                     NATPPKVLEYTVFITESWRHIYELGDGYYIMHPWLLLNPMILGTCVFGVPFLLWRVRT
                     SVAAQLLLGGLGVVTVAVYVPPVATFVGDHLIVPGLLWRLAWPIPLLALITTGWMLWE
                     ALSYAEARLRGLGIGRSVTRTLPLALVALLTAAAAPPSVEKAVGLYRKFEIARTSNYD
                     PDPIYPWIRGHVQDPGVLLARDSANNIVPAYTTSLNVVSQRGEGMIRDRDELEKLAGP
                     HIHIPQRYLDVHSFFFGPTLDEEAYDILHRYHADYLMVYAGSPLDERLKTLPGFSSED
                     NAPREKYSLYNVDLEKLGEPARRSDRPRETAPPEPS"
     gene            7348..8325
                     /locus_tag="VNT12_03555"
     CDS             7348..8325
                     /locus_tag="VNT12_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWI45224.1"
                     /translation="MEIFENLSASSRLEEAELDAYSRAVRDVTERLEPAVISVGLPDG
                     RGGGSGVILGLEGDSATAITNSHVVRGLRSRRAGNPGGDIQVTLSDGSSVSAEVLGDD
                     PPSDLAVVRFDTEEEPTVATLGDAGNLVVGQLVVAIGNPLGFQRSVTAGVVSALGRSI
                     RGESGRLIENVIQTDAAINPGNSGGPLANASARVVGINTAIIGGAQGIGFAVPVSGAF
                     RRVVFTLVTEGRVRRAYLGVVVESRQARGEFEGGAGVRNIAPNSPAERAGVKPGDVII
                     GLKGEPVHSNDDLLNLLDASSIGQDTTLRVLRGNRELSLSVRPREQPDE"
     gene            8522..8755
                     /locus_tag="VNT12_03560"
     CDS             8522..8755
                     /locus_tag="VNT12_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HWI45225.1"
                     /translation="MPDVTFVSDVTGAEESEKLERALTRLGFVDLVNVDSERGLVAVS
                     YEGGEEELGKIEGAIEEAGHNAEPSPGADPDVG"
     gene            8759..9184
                     /locus_tag="VNT12_03565"
     CDS             8759..9184
                     /locus_tag="VNT12_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45226.1"
                     /translation="MPEPSVSVSEAARFLDIPQEEARKLADAEGNVPLETLRSRAGAA
                     TIARELASAREEVGRLSGQLREVRASNVRLQKELRGSVEERRRLTEEVLELRAAAEER
                     LMLMERIEHIARVEQELEESSSELERLRNRRLLGRLLNR"
     gene            complement(9194..9892)
                     /locus_tag="VNT12_03570"
     CDS             complement(9194..9892)
                     /locus_tag="VNT12_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWI45227.1"
                     /translation="MEFMSGEWFGRLLGVLVVNLILSGDNAVVIAMAARTLEGANRKK
                     AIIWGAAGAVVLRLLFAGVITLLLEVPFLQVVGGLLLVWIAWGLVHEDDDDDEEKVKA
                     GSSTWQAVRIIIVADAVMSLDNVIALVQAARIGGEVDISLLVIGLATTIPLVIFGAAL
                     LTSLLNRFPILVYAGAGLLVYLAVEMLFADKVTHPYLKHYESIEWIIGLGAAGIFLVV
                     AWLWARRSSSEETG"
     gene            complement(9977..10996)
                     /locus_tag="VNT12_03575"
     CDS             complement(9977..10996)
                     /locus_tag="VNT12_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45228.1"
                     /translation="MTIIELVLSLAAILGAAILFTNAVEIMGDRLNLGAGAVGSVLAA
                     VGTALPETMIPLVAIFGALLLGSGSAAAGEIGIGAILGAPFLLATLALFIVGAAALGY
                     KGRRETGSEITADRQTAIPDLMFFLLCFIPVTAAGILFLPLLLKIGLSVLLLVAYVLY
                     VIRTVKKGGEAEEDVPARLTLWPFRSQGPTWAVAVQLIGTIIVMGFGAHFFVEAVEHL
                     SKSVGISAGLIALVLAPLATELPEKFNSVIWLRDNKDTLAIGNITGAMVFQSTIPVSI
                     GVLFTPWRLDFISAVAAIFAILSSIVFLGFLLRKAPLKAFYMLGAGSLYIVFVVTAII
                     DLASG"
BASE COUNT         2234 a         3516 c         3578 g         1890 t
ORIGIN      
        1 atgatcgtgg atgggggctt aacagccagg tgaacgagcc acacaggcgc gcggtcgagt
       61 ctggctacga cttgatggcc gaacagtacc tcgccaccaa agatcccgaa gatccgctcg
      121 cactgagggc cctggaggat cttgcctcac tcttgccgtc ggaagccgct gtgctggatc
      181 ttggttgcgg tgctggcgtg cccgttaccc gctggctcgc cgatagaggg ttcaccgtaa
      241 caggtgtcga cgtctcggca aagcaactgg agttggcacg tgcaaacgta ccagacggca
      301 tcttcctcaa agctgacatg accgaagtgg ttttcgggcc cgagtcctta gacgccgtgg
      361 tcgccttcca ctcgatcatc cacgtcccac ggggcgaaca tcgcactctc ctggagaaca
      421 tcaaccgctg gctcaagcca ggaggcgcac tccttgcgac catgaccgta acggactacg
      481 agggccacga ggaggactgg gaacgatggg gggcgccgat ggtgtggagt cactacgaca
      541 ggaatgccaa cgtggcgatg ctgaaggaca ccggcttcca gatacggtac gccgagcctc
      601 gcacaggcgg gggtacgggc gatgagacgg agacctggct ctgggtcctc gccagcaaga
      661 gatcgagaga aggtgcgacg tgaccaccat aaccaaggca gtagccagcg acatccgctt
      721 cccgacgtcg ctggacctct ccggctccga cgccatgaac gaggctcccg actactcggc
      781 cgcctacgtc gtgctccaga cggacggtgg gctcgaaggc cacggcttca cctttaccat
      841 cgggcgcggt aacgagctct gcgtcgccgc gatcagagca ctcgcgccgc tagtcgaggg
      901 gagaaccctc gagtcgttca cccgcgacat gggcgctttc tggcgtcacg tcacgggaga
      961 cagccagctc aggtgggtcg gtcccgagaa gggtgtgatc cacctcgcta cggccgccat
     1021 cgtcaacgcc gtctgggacc tctacgcgaa ggccgagcac aaaccactct ggagactcct
     1081 ctcggacatg agccctgagg agctcacttc ttgcatagac ttccgctaca taaccgacgc
     1141 gatcacacgc gaggaagctc tcgagatcct gcgccggaac gaaccgacga agccggaccg
     1201 tgaggccgag atgctggagg acggtttccc cgcctacacc acgtccgccg ggtggctagg
     1261 gtattcggac gagaggctgc gttcgctcgt gcgcgaggca gtcgggaagg gctggacgca
     1321 cttcaagatg aaagttggcg gggacatcga ggacgacgtg cggcgggccg ccctgatccg
     1381 ggacgagatc ggacccgacc gcaccctgat gatggacgcc aaccaggtct gggacgtgga
     1441 ccaggccatc gagaacatgg agcgtctcgg tgagttcgaa ccgtattgga tcgaggagcc
     1501 cacgagcccc gacgacgtcc ttggccacgc gcgcatagcc gaggctgttt caccgttcgg
     1561 tgttgccacg ggcgagcact gccagaatcg cgtccttttc aagcagctct tacaggctga
     1621 tgccatctcg ttctgccaga tagacgcctg ccgcctcggt ggcgtcaacg aggtcatcgc
     1681 cgtgctcctg atggcggcaa aattcggcgt gcccgtctgc ccgcatgcag gcggcgtggg
     1741 actgtgcgag tacgtccagc atctctccat attcgactac atctgtgtcg gagcctcgct
     1801 cgaggaccgg atccttgagt acgtggatca cctccacgag cattttgttg atccagtgga
     1861 gatccagggc ggccgctacg tcccgccaca agcctccggc tactccgccg agatgaagcc
     1921 cgagtccctg aacgagttcg agttcccaga aggcagcgcc tgggccacag gggagaagag
     1981 catccgatga acgggagagt agcagcggta ttcggggtcg gtcccggact cggggctgtg
     2041 gtcgcccgcc gcttcgcagg cgaaggattc gcggtcgcga tgatggcccg ccgggaagaa
     2101 agccttgcgg agatacgaca ggacatcgag gacgacggcg gtacggcact acctgtctcc
     2161 gccgacgcca cggacgcgga ctctgtcgca gtagccttcg agggaatacg ctcggatctc
     2221 ggtgaccctg aagtcctggt ctacaacgcc ggcgcgttcc agatgggcgg cgttctggag
     2281 atttcgcccg cgcagttcga cgagtgcttc agggccaact gcgcaggggc tttctacgcc
     2341 gcgcgtgagg tactgccggc gatggtcgag gcgggacgtg gcaccatcct gctaacgggc
     2401 gcatcagcgg cgctgcgcgg caaggcgagg ttctccgcac tcgcggtcgg caagttcggc
     2461 ttgagggcgc tggcgcagtc catggccagg gagttcgggc cgcagggaat tcacgtctcg
     2521 cacgtcatta tcgacggcca gataaacaca cccagtatcc gtgagatgat gcccgaccgc
     2581 gaggagcaca ctatgctctc ccccgacgcc atcgccgaaa catactggca actccactcg
     2641 caagatagga ccgcctggac tctggagtcg gacctgcggc cctcggtaga gagcttctga
     2701 ccttaccgcc caccggccac gcggccagcc accgacgaga aggcacgacc ctccatcgta
     2761 tcgtccaggg gttcgagctc gaaactctcg tccacccggc gcagggcgtt caggatgagc
     2821 tgatctgtga gccaggggtt cttgggaagt agggagccgt gcaggtaggt gccgtaggcg
     2881 ttcagcctgc gtgcaccctc ggtaccgtct ttaccgttat tgccgtaacc cacaacgact
     2941 tcgccgagag gtttcaggtc ctcgccgagg taggtgcgac cgccgtggtt ctcgaagccg
     3001 actatctcgc gtgtctcgcc ggtctcaggg gactggacgc ggaccagcac gttcccgatc
     3061 aggcgctcct cgttagcctt cctaggctcc gtgtgcaggt cgaagatgcc tacgccaggg
     3121 agcttctcgc ctactggcgt ctcgtaagag tgacccatga gctggtagcc accgcacacc
     3181 ccgaggacca caccgccatc ctccacgata gccctcaggt ccccgccttt ggagccagag
     3241 agatcttctg cgagcagcgc ctgttccctg tcctggccgc cgccaaagag gaagagatca
     3301 cagccggtcg gccttatctt ctctgtgagg ccgacgtcga cgacctcaac agcaataccg
     3361 cgccactcgc agcgcttctc gatggagata acgtttcccc tgtcaccgta gaggttcatc
     3421 atgtcggcgt agaggtggtg gatagtgagc atgtgcctcc cttcggtcgg cattacagcc
     3481 cgtcgcctgc aaggcggctt gctgcaatgc taatcttccc aaaacggatg cgtgtagccc
     3541 atctcgctca gggtctgccg gatctccagc attgccgtgt acgtaggcag cacgtagagc
     3601 gtttcgcctg gcggggtcgc cgcgagtgca gactttatgg cctcctcgcg gtcggggatg
     3661 acggggccta cagggagctc ggcgtacttg aggcgcacgg ccatgtcttc ggccctgatg
     3721 ccgctgacgg tgaacggggt ggcatccgct ttgccctcct ttctcgcgtc ggcgagcatc
     3781 tcgaagtcca catcccagag ccaggagaca tcgcgtccgt cggcgtcatt gtcgtttatg
     3841 gcgatgagga catttttggc gtcggcgccc gtgacgaagg tccgcaggat ctcgttgaac
     3901 cccacagggt tctttatgag cagcaagaag gcctctctgt cccccgcctt tacccgctcg
     3961 accctcccga aggcaccccc gaactcactg aggccgcggg agatctcagc gagcccgacc
     4021 ccggcctccc cggccaccgc ggcagcggcg agggcattgt agacattgta gagaccaggg
     4081 agtcctagct tcacgttcgc cttgtccttc ggggtcgaga gcaggaactc cgacccctgg
     4141 gctccttgga gccgcaccct gctcgcacgg taggctggct ccggccgtga gaaatcgcag
     4201 tggcggcagt cgtagaggcc gacgtgtccc atgtatacgg tctcgtagac gagggccgtc
     4261 ccgcacacgg gacagtcctt cgagtcagcg acgtgctgaa ggcgcccggt atccagagaa
     4321 ggctcgtcca ccccgtagaa ggcgggtcgc ctggcggacc gcccgaggct cgcgacgagg
     4381 gggtcgtccg cgttcaggac caccgcgccg ctctgtggca ggtcaccgaa ggacgaggct
     4441 atgaccttgc ccgtgtaggc aagctccccg tagcggtcga gctggtctct gaacaggttc
     4501 agaacagcga ggatctcgat ctccgcctcc gcggcaacct tcggcacgct ggcctcgtca
     4561 acctcgaaga gccccatctc cgagagaggc gcccctgcca gccccgagtc cgagacgagg
     4621 gccgcggtca caccggtaac caggttcgcc cccgtcgagt tgttcacggc acggacacct
     4681 gcgatctgga ggattttaga caccatcctg gtcgtagtcg ttttgccgtt ggtgcccgtt
     4741 atggccgccg acccgagccg caggcgccgc gagagcttgg tcaacacttt cgggtcaacc
     4801 ctgcgcgcca ccactcctgg caccgtcgag cctccgccgg ttttcagcgt cctgctcgcg
     4861 aaccgggccg acctggcggc cagtatcgcg gcggagagac gtgggtcggg taagcggctc
     4921 atggcgcgga ttatatggtc ctgggctgga tcttgcggaa gcatctctct acgggttctc
     4981 cactctcacc ggagccctgg ctcctcagga tggctccggc ggagcggtct cacgaggtcg
     5041 gtcggatctc cgggctggtt cgcctagctt ctcgaggtcc acgttgtaga ggctgtattt
     5101 ctctctgggg gcattgtctt ctgaggagaa gccaggcagg gtcttcagcc gctcgtcgag
     5161 tgggctgccg gcgtagacca tcaggtaatc tgcgtgatag cggtgcagta tgtcgtaggc
     5221 ttcttcatcc agggtgggac cgaagaagaa gctgtgtacg tcgaggtagc gctgagggat
     5281 gtggatgtgg ggcccggcca gcttctccag ctcatcgcgg tctcttatca tgccctcgcc
     5341 ccgctgactt accacgttca gcgaggtcgt gtacgcgggg acaatgttat tcgcgctgtc
     5401 gcgcgcgagc agtacgccag ggtcttgcac gtggcccctg atccagggat agatgggatc
     5461 agggtcgtaa ttgctcgttc gcgcgatttc gaacttgcgg tacagtccca cggccttctc
     5521 cacggaaggc ggcgcggcgg cggccgtcag gagggcaacc agcgccagtg gcagggtcct
     5581 ggtaacgcta cgaccgattc ctaaccccct caagcgcgcc tcggcgtaac tgagggcctc
     5641 ccacaacatc cagccggtgg tgatcagggc gagcagcggt attggccaag cgagacgcca
     5701 gaggagtccg ggaacgatga gatgatcccc cacgaacgtg gcgacgggag ggacatagac
     5761 ggccacggtt acgacgccga gacctccgag gaggagctgg gcggcaacgc tcgttcttac
     5821 ccgccacagc aagaaaggca caccgaaaac gcaagtgcca agtatcatgg ggttgagcag
     5881 cagccagggg tgcatgatgt agtagccgtc gccgagctcg tagatgtgcc tccagctctc
     5941 cgttatgaag accgtgtatt cgagcacctt ggggggcgtc gcgtttatgt cagcggagaa
     6001 cagcacggcc tccggagacc tgcccgcaaa cagcaagagt acgactggcc cgagggcgac
     6061 gctccacatc ccgagcgcga ggactaccat cccggtccag gcggccctca ggcgcaagtt
     6121 taccgccacg tgcgccaccc cgaagccgag catgcacaac ccgatggccg gcagtacaga
     6181 aggatgcacc gctactgcag cccagcagaa gaacgtgaag aggccgagat gcctccacct
     6241 cctgctctcc acgaagagca aggcgaagag cagcgttatg ggtaggatca ggaaccttgc
     6301 tgcgtacttg tcctccgaga tacgggctgc cagctccacc cctacgttgt gtacggaagg
     6361 ctggataaag atgatgtgga acagggcata gacagaggcg atgagaactg cggcccgctc
     6421 gctcttcgag atggcaagag ccaggacata gaccaccaac agggccacta caacgagcgt
     6481 gggcgtaagg tatctgagca ccagttcgat cgtgtccagg cctgaaacgc gggatagggc
     6541 tgcctgttcg agcaaccatc cgttgacctt cgcgcgggag aactcagcga tcctctcccc
     6601 aaagtaaggg tccaccaacg cgagcctctc ggagttcgcg aagtccttca cccacgacag
     6661 atagacccaa atgtcgtcgt agctgctagg cacccgcctg gtccccacga acgccagaac
     6721 cccgcagagc aacgcgaaag gcgcccacaa ccagcccgcc ggaggaccat aaggagcgtc
     6781 cctctcggga gaggactcca ccgtgagttc cttcgggcgc acggttctcc acaccgccac
     6841 cgcgagaaag gcggctagca acgtccccgc agccaggaga tagttctcgg tggtgaggtg
     6901 caagatcagg gccggcacac caaggagccc gaagatgccg gtgctgatcg cgaatcccac
     6961 gggaaccagg gcggtccccg acatgtcgtc cctgagaagc cagtgcgaca ggagcaaccc
     7021 cggcgccgtg aagagtacga gggtggcggc gaaggcaaga ggagggaaga ctcccgagag
     7081 ctccctcagg ggaagaagca ggatcaccgc cgccacgcac gagagcaaaa cgagagcatc
     7141 cggcgatctc agacgagtca agccgctgct ccgtcgcgag aatccacctg gagaccgagc
     7201 cttcatgaac cgcgtgtggg tgtgacgcgg gcctcagacg tttccatcgg cgaaagctaa
     7261 cacactctct cccctgtcac ccgagccttg cgatacctgg tcgttcttcc gggtggcgcc
     7321 gtcccggcgt tgcggtacaa atagcgcatg gagatcttcg agaacctttc ggcgagttca
     7381 cggctcgagg aagccgagct cgatgcctac tcgcgggcgg tgcgggacgt aacagagaga
     7441 ctggagccgg ctgtgatctc ggtcggctta cccgacggac gcggtggcgg gagcggggtg
     7501 atactcggcc tcgaaggcga ctcggccaca gccataacca acagccacgt cgtgcgcggc
     7561 ctgcgtagcc gcagggctgg caacccgggc ggggatatac aggttacgct ctcggacggg
     7621 tcatcggttt cggcagaggt gctcggcgac gatccgccga gcgatctcgc cgtcgtccgc
     7681 ttcgacaccg aggaggagcc gacggtcgcc acgctgggag acgcggggaa tctcgtagtc
     7741 ggccagctcg tcgtcgcgat cgggaacccc cttggcttcc agcggagcgt cacggcaggg
     7801 gtagtcagcg ccctcggacg ttctatcagg ggtgagagcg gccggctcat agagaacgtg
     7861 atccagaccg acgcggcgat caaccccggc aactcgggcg gcccgctggc caacgcatca
     7921 gcaagagtcg tcggcatcaa cacggcgatc atcggcggcg cgcagggtat agggttcgcc
     7981 gtccccgtct cgggcgcgtt ccgccgcgta gtcttcacgc tcgtaacgga gggacgggtg
     8041 cgccgcgcct acctcggggt ggtggtggag agccgccagg cgcgcggcga gttcgagggc
     8101 ggcgccggcg tgaggaacat cgccccgaac agcccggcag agagggccgg cgtaaaaccg
     8161 ggagacgtca tcatcggcct gaaaggggaa cccgtccact cgaacgacga tctccttaac
     8221 ctgctggacg catcgtccat cggccaggac accacgctac gcgtgctgcg cggcaacagg
     8281 gaactgtcgc taagcgtgcg gccccgagaa cagccagatg aatagcaggt cagcatttca
     8341 gctagaacgt ggtcggcgaa cagaggagat gacaactgga cgaagatgga ccaaactaat
     8401 gttcttgaca gagcggcgta accgtgtagc gatctcgccg cttcgacaga aagctgaatt
     8461 gctgacaagc taaggcgaag cctgagcgtg ctgaaatgct aagcgaccga agggagcgcc
     8521 aatgcccgac gtgactttcg ttagcgacgt tacgggcgcc gaggagtccg agaagctgga
     8581 gcgggccctt acgcgcctgg ggttcgtgga cctggtgaac gtggactcgg agaggggact
     8641 cgtcgccgtc tcctacgagg gtggcgagga ggagctcggg aagatcgagg gcgccatcga
     8701 ggaggcaggc cacaacgccg agccttcccc cggcgccgac cccgacgtgg gctgatccat
     8761 gcccgaaccc tccgtctctg tatcagaagc ggcccgcttt ctggacattc cgcaagagga
     8821 agcgagaaag ttagccgacg cggagggcaa cgtcccactg gagaccttga ggagccgggc
     8881 cggagcggcg accatcgcgc gggagctagc cagcgcccgc gaggaggtcg ggaggttgtc
     8941 gggccagcta agggaggttc gcgcttccaa tgtacgtctc cagaaagagc taagaggctc
     9001 ggtcgaggag cgcaggcgcc tgacggaaga ggtgctggag ctgcgggccg cggccgagga
     9061 gaggcttatg ctcatggaac gcatcgagca catagcccgt gtcgagcagg aactggagga
     9121 gagctcctcc gagctggaac gcctcaggaa caggaggctc ctgggtaggc tcctgaaccg
     9181 ctagagagtc tccctaccct gtctcctcgc tgctcgatct tcgtgcccac agccaggcca
     9241 cgaccaaaaa gatgccggct gcgccgaggc cgatgatcca ctcgatgctc tcgtaatgct
     9301 tcaggtaggg gtgggttact ttgtcggcga agagcatctc cacggccagg tagacgagca
     9361 gaccggcgcc cgcgtagacg aggatgggaa aacggttcaa cagcgacgtc agcaacgcgg
     9421 ccccgaagat caccagcggg atcgtcgtcg ccagcccgat gacgagcagc gagatgtcga
     9481 cctcgccccc gatcctggcg gcctggacca gcgcgatcac gttgtccagc gacatcaccg
     9541 cgtcggccac gattatgatc ctgaccgcct gccaggtgct cgaaccagcc tttaccttct
     9601 cctcgtcgtc atcgtcatcc tcgtggacga gcccccaggc gatccagacg agcagcaatc
     9661 ctcccaccac ctgcagaaaa ggcacctcca gcagcaaggt gataacgccg gcgaagagca
     9721 gcctgagaac gacggcccca gccgcgcccc agatgatggc cttcttccgg ttggcaccct
     9781 ccagagtgcg ggccgccatc gcgatgacca cagcgttgtc gccggagagg atcaggttca
     9841 caaccagcac cccaaggagc cggccaaacc actcccccga catgaattcc atctgacccg
     9901 gcttcctact cgaccatact acaggctgcc tatcttatca ggaaagggga aaaccaagtg
     9961 cgagatccgg tccaactcag ccgctggcga ggtcgatgat cgctgtcacg acgaagacta
    10021 tgtagagtga tcctgcgccg agcatgtaga acgccttgag tggggccttg cgcagcagga
    10081 acccgaggaa gactatgctc gagaggatgg cgaagatggc tgcgacggcg ctgatgaagt
    10141 cgagccgcca cggcgtaaag agcaccccga tcgagaccgg gatggtgctc tggaagacca
    10201 tagcccctgt gatgtttccg atggccaggg tgtctttgtt atccctgagc cagatgacgg
    10261 agttgaattt ctcaggcagc tccgtcgcca gaggcgcgag gaccagcgcg atcaagcctg
    10321 cggagatccc cacggacttg gagagatgct caacggcttc gacgaagaag tgggccccga
    10381 agcccatcac tattatcgtc ccgattagtt gtacggcgac agcccaggtc ggcccctgag
    10441 agcgaaatgg ccagagcgtc agcctggcgg ggacatcttc ttcggcctcc ccgcccttct
    10501 ttaccgtcct tatcacgtag aggacgtaag cgaccagcag cagcacggaa aggccgatct
    10561 tcagcaacag aggcaggaac aagatccccg ccgcagtgac cgggataaag cacagcagga
    10621 agaacatgag gtcggggatg gcggtctgcc tatcggctgt gatctcgctc cccgtctccc
    10681 ttcggccctt gtagcccagg gccgccgccc cgacgatgaa cagggctagc gtcgcgagca
    10741 ggaaaggcgc gcccaggatc gcgccgatac ctatctcgcc tgctgcagcg ctgcccgagc
    10801 caaggagtaa ggcaccgaag atagcaacca atgggatcat ggtctcaggt aacgccgtgc
    10861 cgacggccgc cagcacgctc cctacggccc ccgctccgag gttcagccgg tcgcccatga
    10921 tctctacagc gttggtgaaa aggatcgcgg cgccgagtat cgccgccagg gacaggacca
    10981 actcgattat cgtcaagggc tctccttatg accactggag gtaggaacgt gctagattct
    11041 tacccgctgg caagcttact atacccggaa gcgggagcgt ggacgaactc gagaaaaccc
    11101 tggcagcggt cgcgagcggc gaactctctg tagagtcggc cgccggacgc ctgaagaggg
    11161 gcgaggtccg ctacctcggc gagttcgccg ccctcgacct cggccgctcc atcagaga
//
LOCUS       DATMJA010000153         7192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_790054, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000153 DATMJA010000000
VERSION     DATMJA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790054"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VNT12_03580"
     CDS             <1..698
                     /locus_tag="VNT12_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45229.1"
                     /translation="HPEGRKEWPYVGITVALMRAFGVEVDRANGRFTVASAMYSSREY
                     EVEPDASGASYFMAAAAVTGGRVRIPGLGSSSPQGDLRFAGVLRDMGCRVEITPDFIE
                     VQGPDRLQGVEVDMNAFSDTMITLSAIAPFATTPTTIKNVAHSRLQETDRLWAVATEL
                     NRLGIKTQTTPSSIRIIPGQIQTGVVRTYGDHRMAMAFAVTGLVASGIRIGDPGCVTK
                     TFPGYFGALASLR"
     gene            733..1398
                     /gene="cmk"
                     /locus_tag="VNT12_03585"
     CDS             733..1398
                     /gene="cmk"
                     /locus_tag="VNT12_03585"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWI45230.1"
                     /translation="MGGILVAIDGPAGSGKSSVARAVARRLGVVNLNTGAAYRAAALL
                     ALREDVDLADGEVLAALARRVELDPQGASIDGGPIGERDLRSPEVSAAASKVSAHAPL
                     REVLLPVQRAAAEKARATGGAVVEGRDIGTVVLPDAELKVYLSAAPEERARRRASQTG
                     REGELDRIRAAIARRDRQDSEREISPLKPAADAVVLDTTSLSLEEVISRVLDLATDLR
                     ERR"
     gene            1395..2048
                     /locus_tag="VNT12_03590"
     CDS             1395..2048
                     /locus_tag="VNT12_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWI45231.1"
                     /translation="MRVAMLFEGRRKPGEMSGRYRLFRRAMTLLCRLLFGYRVHGGDR
                     VPSDGPLIVASNHSQYADPVLVCVAVPRRLQWMAKKQLFVFPFRKFFEFIGSFPVDRE
                     GGGRGAIRAALAFLAEGWALGIFPEGTHRGAGASRETKSGVVVLAVRSGASVLPVHVG
                     RLPGPLSRLRGQRLHIFVGEPVDLDSAMRGGAAYRAAADEIMHTIYALPERHAQESL"
     gene            2045..3349
                     /gene="der"
                     /locus_tag="VNT12_03595"
     CDS             2045..3349
                     /gene="der"
                     /locus_tag="VNT12_03595"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWI45232.1"
                     /translation="MNRLPVVAVVGAPNVGKSSLVNRIMRRRGAVVSERPGTTRDRAY
                     NRAEWAGREFTLVDTGGMEPRAKVGLEALVTLQARVAVEEADVVLHLTDARLGPTEAD
                     AVIARELRRARGKAVLAVNKLDNPADDTERHSFYSLGEGEPYAISAVHGLGVGDLLDV
                     VVSLLPESEPESEEEPPALAIVGRPNVGKSTLLNRLLGTDRAVVSEKAGTTTDAVAAE
                     IALEAEGETERYLLLDTAGVSKGARRARGVPYYSSLRTSEAIRRSDVSLLLVDATDGI
                     VAGDLRIAREIEEAGSSCGVVVNKRDLVPPQRLREIEGLVTARLTDLKPQAVHISALT
                     GAGVDEVLPFAARVGRAYRRRVPTHELAEFVSDVVGRSAPPKGVKIRYATQTRTAPPR
                     FTLFANRPKDLPESYLRYVENALRERYGLWGVSIRLRVKSSR"
     gene            3649..6165
                     /locus_tag="VNT12_03600"
     CDS             3649..6165
                     /locus_tag="VNT12_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWI45233.1"
                     /translation="MKAIIMAGGQGTRLRPLTSDQPKPMIPIVNAPCMEHIVRLLERH
                     GFTDIAVTLQFLPDEIRDYFGDGSDWNVNMHYSIEDAPAGTAGSVKMAEQQLRLEGER
                     LLIISGDALTDADLGTLVEFHEDKGSEATMVLKSVENPLDFGIVITEEDGRISRFLEK
                     PAWGQVFSDTVNTGIYLLEPSATAEIPDPEEGEYDFSKELFPKLLDAGRPLYGYITED
                     YWEDIGTLEQFASAQRDVLDGRVKGIRPPGTRLRENIYVGRRAQVDDDELEGPVVIGE
                     NARIDDGARIGPYSVIADNVVVSAGATVERSVIAEGTYIGEGAELVDTLVGRNSYVQA
                     RARILERSALGDDVIVGEGATIAPEVKVYPHKTIETGANVTQSLIYETMGLRTVFKRG
                     VVSGKFNVDVTPEFVTRLASSFGTTLDPGAVVTLGRDSSLSAQVAKRAMTAALLGTGI
                     NIRDLRAAHAGVVRHDVLAGKSSAGAHVRAGSDPDDVEILFFSADATPITEGDERSVE
                     KAFVREEYRRAYGGDIGELIYPGRVVEQFVERLERAVDREKTSGATIVVDFCGGVTGL
                     VASRVFSRLGVSAVVMEGFANANAVGEVGRMNLEESIERVARIVPTVDAAFGAVVGPE
                     AEYVQFVDDEGEWVPPDVMLACLIGQMHPQSAVLPINLSRGYEKLVEEGEGAVEMSRT
                     GLSNIAIKAADIGADLASLADGRYILPAFLPAPDCFMTLAIALEVFRETPLSGVRKKF
                     GASFDQVHREQIECPWSAKGRVMRGLAEKFGADPDAILAEGVLLNVEDGWVLMLPDPD
                     SPMFYVYAESTEGDGSPAQLMEEYVDLVKSLIDDQALLSS"
     gene            6204..6464
                     /locus_tag="VNT12_03605"
     CDS             6204..6464
                     /locus_tag="VNT12_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45234.1"
                     /translation="MDDSGLRGGGFDWENAEDVTALSEEDLRERLEALSEEERAVSYR
                     RRILQGRIDLIRTELVERGAVALPPERLARVLLGDSPDEETR"
     gene            6461..6910
                     /locus_tag="VNT12_03610"
     CDS             6461..6910
                     /locus_tag="VNT12_03610"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWI45235.1"
                     /translation="MKECPECGAEISAAMKFCTECGTKLDLGQSTVSYAPSFGEDVEV
                     GTSGGAEGAALIEMDQVEGTAGRRMHDLGDDVVSVGRGQESSIFLDDVTVSRKHAEIV
                     HGDGGYRIRDVGSLNGTYVNRVRVDAVDLRNGDEIQVGKYRFRFVFS"
     gene            6942..>7192
                     /locus_tag="VNT12_03615"
     CDS             6942..>7192
                     /locus_tag="VNT12_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWI45236.1"
                     /translation="MNRVENNGIDREKGEGRTTEGYAPAQERYSATKDNFTIGEVVER
                     LRPEFPALSVSKIRYLERRRLINLSRTRGGYRLFSSQDI"
BASE COUNT         1405 a         2148 c         2410 g         1229 t
ORIGIN      
        1 tccaccccga gggcaggaaa gaatggccct acgtcggtat caccgtggcc ctgatgcggg
       61 ccttcggcgt ggaggtagac cgagcaaacg ggcgcttcac cgtcgcctcc gccatgtact
      121 cctcgcgaga atacgaggtg gagcctgacg cctcgggggc gtcctacttc atggcggcag
      181 cagccgtaac tggtgggcgc gtgaggattc cagggctcgg ctcatcttcg ccacagggtg
      241 acctacgctt cgcaggggtg ctgcgtgaca tgggctgcag ggtcgagata acccctgact
      301 tcatcgaagt acaagggccc gaccgtctac agggtgtcga agtggacatg aacgcgttct
      361 ctgacaccat gatcacactc tccgctatcg ccccgttcgc cacaacccct accaccataa
      421 agaacgtagc ccatagccgc ctgcaggaga cggaccgcct ctgggccgtc gcaaccgaac
      481 tgaatcgcct cggcataaag acacaaacta ctccatcgtc tatccgcata atacctggtc
      541 agatacagac tggagttgta cggacgtatg gcgaccacag gatggcgatg gcgtttgcgg
      601 taacgggctt ggttgcgtcg ggcatcagga tcggagaccc tgggtgcgtg acaaagacct
      661 ttcctggcta cttcggggca ttggcatctt tgcggtgaag cggcggggct ttcggctata
      721 cttcctgggt cgatgggagg cattctcgta gccatagacg gtcccgcggg gagcgggaag
      781 agttcggtcg cgcgagccgt tgcacggcgc ctcggggtcg tcaacctcaa cacgggcgcc
      841 gcctacaggg ccgccgcgct cctcgccttg cgcgaggacg ttgaccttgc cgacggggag
      901 gtgcttgcgg ccctcgcgcg cagggtggag ttggatcccc aaggagccag catcgacggg
      961 ggaccgattg gggagcggga tctccgctcg cccgaagtct cagcagcggc ttcgaaggtc
     1021 tcggcccacg cccccctcag agaggtcttg ctccccgtgc agagggcggc ggccgagaag
     1081 gcaagggcca ccggaggagc cgtcgtcgag ggaagagata tcggtaccgt cgtccttcct
     1141 gacgctgagc ttaaggttta cctatccgca gctccagaag agcgggcgcg caggcgggcg
     1201 tctcaaacag gcagggaggg ggagctcgat cgcataaggg ccgccatagc caggcgcgac
     1261 cgccaggatt ccgagcgtga gatctcacct ctcaagcccg ctgctgacgc cgtggtctta
     1321 gacaccacct ccttgagcct tgaagaagtc atatctaggg tcctagatct cgccacagac
     1381 cttcgcgaga gaaggtgagg gtcgccatgc tcttcgaggg gaggcgtaaa ccgggcgaga
     1441 tgtctgggag gtaccgcctg ttccggcggg cgatgaccct gctctgccgc ctcttgttcg
     1501 ggtacagggt gcatggaggt gacagggtgc cttccgatgg gccccttata gtggcctcca
     1561 accacagcca gtatgccgac cccgtcctgg tctgcgtggc ggtcccccgt cggttgcaat
     1621 ggatggcgaa aaagcagctc ttcgtcttcc cattcaggaa gttcttcgag tttatcgggt
     1681 cattccctgt ggaccgcgag ggcggaggaa ggggtgcgat ccgcgccgcc ctggccttcc
     1741 tggccgaagg ttgggcattg gggatcttcc ccgaaggtac ccacaggggc gccggagcct
     1801 ccagagagac caagagcggg gtcgtcgtgc tcgcggttcg cagcggcgct tccgtgctgc
     1861 ccgttcacgt aggcaggcta ccaggaccgc tctcacgcct gcgcggccaa cggctgcaca
     1921 tcttcgtggg cgaaccggta gacctggact cggctatgcg gggtggtgcg gcgtaccgcg
     1981 ctgcggcgga tgagatcatg cacaccatct acgctctgcc agaacgacac gcgcaggagt
     2041 cgctgtgaac cggctccctg tggtcgccgt cgtcggggcc cccaacgtgg ggaagagctc
     2101 gctggtaaac aggatcatgc gccgccgcgg tgccgttgtc tcagagaggc cgggcaccac
     2161 ccgcgatagg gcgtacaacc gggcggagtg ggctggcagg gagtttacgc tggtggacac
     2221 gggcgggatg gagccgcgcg caaaggtagg actcgaggcc ctggtgacgc tgcaggcgag
     2281 ggttgctgtc gaggaggctg acgtggtgtt gcacctcacc gacgccaggc tagggccgac
     2341 cgaggccgac gccgttatcg ccagggagct caggcgcgcg cggggcaagg cggtgctcgc
     2401 cgtcaacaag ctggataacc ccgccgacga caccgagcgt cacagcttct actccctcgg
     2461 tgagggcgag ccttatgcga tctcggcggt gcacggcctg ggtgtcgggg accttctcga
     2521 cgtcgtcgtc tccctgctgc cggaatcaga accggagagc gaagaggagc cgccggcgct
     2581 cgccatcgtc ggcaggccga atgtcggcaa gagtacgctt ctcaatcgtc tgctcgggac
     2641 ggaccgcgcc gtcgtttcgg agaaggccgg cacgacgacc gacgccgtcg cggctgaaat
     2701 agcactcgaa gcagagggag agaccgaacg ctatctcctt ctcgatacgg caggcgtaag
     2761 caagggtgcc aggcgggctc gtggcgtgcc atactactca tccttgcgga cctcagaggc
     2821 gataagacgc tccgacgtct cgctgctgct ggtggacgcc acggacggca tcgtggcagg
     2881 cgacctcagg atcgcccgcg agatagagga agctggctcc tcttgcgggg ttgtagtaaa
     2941 caagcgagac ctcgtacccc cacaaaggct gcgggagatt gaggggctcg ttacggcccg
     3001 cctcaccgac ctcaagccgc aggccgttca catctcggcg ctaacgggcg caggggtgga
     3061 cgaggtcttg cctttcgcgg ctagagtagg gagggcatac aggcggcggg taccgacaca
     3121 cgagcttgca gagttcgtca gtgacgtggt agggcgcagc gcgccgccga agggggtcaa
     3181 gatccgctac gctacccaga cacgcacggc gccaccgagg ttcacgctct tcgcgaacag
     3241 gcccaaagat ctgcccgaga gctatctgcg ctacgtggag aacgctctcc gagagaggta
     3301 cggactctgg ggtgttagca tcaggttaag ggtcaagagc agcaggtagg aagttcgaaa
     3361 tgggggttta ccctcatttc gaacgctcgg gcgacttacg tgtgcgaaag ctgaagtgct
     3421 gaaatgctga ccagcgcccg gctgggcgcg tgctgacaag gcgcgaagcg ccgtgctgac
     3481 acgcgaagcc ggagcgtgct gataaactct tgtcgggaaa gcaaggcatc ggccttgtcg
     3541 agacgcggga aggatcagga gcgaacaagg agcgtacatg ttcgacgtgg cggctagatc
     3601 agagcactta aggcgttggc ggttcgtccc agccaggaag gaactcagat gaaggcgatc
     3661 atcatggccg gggggcaggg aacgcgcctt aggccgctga ccagcgatca acccaagccg
     3721 atgattccca ttgtcaacgc cccttgcatg gagcacatcg tgaggctcct ggagcgccac
     3781 ggcttcacag atatcgctgt gaccttgcag ttcttgccgg atgagataag ggactacttc
     3841 ggtgacggtt cggactggaa tgtaaacatg cactactcca tcgaggacgc cccggccggg
     3901 accgcgggtt ccgtgaagat ggccgagcag caactccgcc tggaaggcga gcgcctgctc
     3961 atcataagcg gcgatgccct taccgacgcc gacctgggca ctctggtcga gttccacgag
     4021 gacaaaggct ctgaggccac catggtcctc aagagcgtcg agaacccgct ggatttcggc
     4081 atcgtcataa ccgaggaaga tgggcgcatc tcgcgctttc tggagaagcc ggcttggggg
     4141 caggtcttct cggacaccgt aaacacaggc atctatctgc tcgagccctc ggcgacggcc
     4201 gagatccctg accccgaaga gggagagtac gacttctcca aagagctgtt ccccaaacta
     4261 ctcgacgccg gacgtccact ctacggctac atcaccgagg attactggga ggatataggc
     4321 accctggagc agtttgccag tgcccagcgc gacgtgctcg acggccgcgt aaagggcatc
     4381 agacctccgg gcacgcgcct gagggagaac atctatgtgg ggcgcagggc tcaggtcgac
     4441 gacgacgaac tcgaagggcc cgttgtgatc ggggagaacg cccgcatcga cgatggcgcg
     4501 aggatcgggc cctactcggt catcgccgac aacgttgtag tctcggctgg cgccacggtg
     4561 gagcgcagcg tcatcgctga ggggacctac ataggtgaag gcgccgagct cgtagacacc
     4621 ctcgtcggcc gcaactccta cgtccaggcg cgggcgcgca tcctggaacg cagcgcgctc
     4681 ggtgacgacg taatcgtcgg cgagggggcg actatagccc ccgaggtaaa ggtctaccct
     4741 cacaagacca tagagaccgg ggccaacgtc acccagtccc tgatctacga gaccatgggg
     4801 ctgcgtacag tcttcaagcg cggcgtcgta agcggcaagt tcaacgtgga cgtcaccccc
     4861 gagttcgtca cccgcttggc ctcatcattc ggcactacgc tcgatcccgg ggccgtggtt
     4921 acgctaggtc gcgactcctc gctctccgcc caggtcgcaa agcgcgcgat gacagcggcg
     4981 ctgctcggca ccgggatcaa tatcagggac ctcagggccg cccacgctgg ggtcgtccgc
     5041 cacgacgtgc tcgccggtaa gtcctcggcc ggggcgcacg tacgtgccgg cagcgacccc
     5101 gatgacgtcg agatcctgtt tttctccgca gacgccaccc ccataacgga gggggacgag
     5161 aggtctgtgg agaaagcctt cgtgcgcgag gagtaccgta gagcctacgg cggagacata
     5221 ggggagttga tctacccagg ccgcgtcgtg gagcagttcg tggagcgcct ggaacgcgcc
     5281 gtggaccggg agaagacttc tggcgccaca atcgtggtgg atttctgcgg cggcgtcacc
     5341 ggcctcgtgg cgagccgggt gttctccagg ctcggcgtga gcgccgttgt catggaaggc
     5401 ttcgccaacg ccaacgccgt cggcgaggtg gggaggatga acctggagga gagcatcgag
     5461 cgcgtcgccc gcatcgtgcc cacggtggac gctgccttcg gcgccgtcgt cggccccgaa
     5521 gccgagtacg tgcagttcgt cgacgacgag ggagagtggg tgccacccga cgtcatgctc
     5581 gcctgcctca taggccagat gcacccgcag agcgcggtgc tccccatcaa cctcagccgc
     5641 gggtacgaga aactcgtgga ggagggcgaa ggcgcggtcg aaatgagccg tacaggcctc
     5701 tccaacatcg ctatcaaggc cgcggacatc ggggctgacc tagcttcact cgccgacggc
     5761 cgctacatcc tccctgcctt cctgccggcc ccagactgct ttatgacgct ggccatcgcc
     5821 ctcgaagttt tccgcgagac gcccctttcc ggtgtgcgca agaagttcgg agcctcattc
     5881 gaccaagtcc acagagaaca gatcgagtgc ccgtggtcgg caaagggacg tgtgatgcgc
     5941 ggcctcgccg agaagttcgg cgctgacccc gatgccatcc tcgccgaggg cgtgctcctc
     6001 aacgtcgaag acggctgggt gcttatgctc ccagatcctg acagccctat gttctacgtc
     6061 tacgccgagt ccacagaggg cgacgggagc ccggcacagc tcatggaaga atacgtcgac
     6121 ctggtgaagt cccttatcga cgaccaggcc ttgctctcaa gctgagattg cacttcaggt
     6181 tcagggtagg ttatactcaa gccatggacg attcggggtt gcgtgggggg ggatttgact
     6241 gggagaacgc cgaggacgtc actgccctct ctgaggaaga tctgagggag aggctagagg
     6301 cgctctcgga ggaggagcgt gcggtcagtt acaggcgcag gatcctgcag gggaggatag
     6361 atctgatccg tacagagctc gtagagcggg gcgctgtggc gctccctcct gagcgcctgg
     6421 cgcgggtgtt gctcggtgat tcgcccgatg aggagacaag gtgaaagagt gtcctgagtg
     6481 tggggccgaa atctcggcgg cgatgaagtt ctgtacagaa tgcggcacca agctcgacct
     6541 tggccagagc acagtctcct acgcgccgag tttcggcgag gacgtcgagg ttggcacatc
     6601 aggcggcgcc gaaggggccg cattgataga gatggatcag gtagagggca cggctggacg
     6661 caggatgcac gacttgggcg atgacgttgt cagtgtcggc cgcggacagg agagcagcat
     6721 cttcctcgac gacgtcaccg tctcacgaaa acacgcggag atcgtgcacg gagacggcgg
     6781 gtacaggata cgcgacgtgg gaagcctgaa cgggacatac gtcaaccgcg tgagggtcga
     6841 cgctgtcgac ctgcgcaacg gggacgagat tcaggttggt aagtaccgtt tcaggttcgt
     6901 gttctcctag agcagcagac accgtaggcg agaggatggg agtgaacagg gtggagaaca
     6961 acggtataga cagggagaaa ggcgaaggcc gcaccaccga aggttacgcg ccagcacagg
     7021 agaggtacag cgccaccaag gacaatttca ccatcggcga ggtcgtcgag cgcctgagac
     7081 ccgagttccc ggctctctcg gtcagcaaga tccggtacct ggaacgcagg cgtttgatca
     7141 acctcagccg cacgcgcggc ggctacaggc ttttctcgtc ccaggatatc ga
//
LOCUS       DATMJA010000154         2810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_612011, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000154 DATMJA010000000
VERSION     DATMJA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612011"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VNT12_03620"
     CDS             <1..689
                     /locus_tag="VNT12_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI45237.1"
                     /translation="LIRPDFVMADATRRGWIHSIRNFVLDGAKYARPRLGPDHYLVVK
                     EPNGSVGAPLLMEALPESRMILLIRDPRDVVSSTLDGARKGNWLYERKDKGGWKRDSL
                     PDENPDAFTRRWANVYMQHAGNAKMAYDAHRGPKVMVRYEDLRADTLGTMRRIYSTLG
                     IPVDGHELARAVEKHSWENIPEWKKGGGKFYRKATPGGWQEDLTPRQIKQIERITAPL
                     LKEYYSGEAP"
     gene            692..1663
                     /locus_tag="VNT12_03625"
     CDS             692..1663
                     /locus_tag="VNT12_03625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWI45238.1"
                     /translation="MAEKVSVVIPTFNAGPGFEELLRKLDAQEGNFEREVVVVDSGST
                     DGTAELAARHGAILHSVSKASFDHGATRDLGISLSSGEYVALTVQDAVPLDERWLAAL
                     VENLQSDGRIAAVYGRHVPRPDAGVITRALVGNLAVSDTERREQEIRDPAHYLSLPPA
                     QRRRIAAFDNVSSCLRRQVWEEFPFGEADFAEDLRWGKKVVEAGHTIVYEPRSVIVHS
                     HERGALYDLRRHYVDQRVLDELFGARLVPNLPRLILGIPYATRHVYGLLKREEPAPEK
                     RPKLLLDAMTYALASQLGAYLGGKLPSLSRLSPQLHDRLHTILGKGV"
     gene            1682..>2810
                     /locus_tag="VNT12_03630"
     CDS             1682..>2810
                     /locus_tag="VNT12_03630"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI45239.1"
                     /translation="MNLERVVERLRDYRLKNERFDRLVGAVLSNEKAMRAARATVAAA
                     RSVSRKGQTVAAGDYDTVFFVAGEMRSGTSWLRHSLSDHPEIACGQEGSFFGRGYDHE
                     EIPVYTGPVSSLTRALALSRELKTWHALPWNLWSDGYDEDLRNLSRLSVDYFLSKEVA
                     RTGRRIVGDKSPQHTENLDEIYEIYPDARIIHIVRDGRDVAVSAMHHWWRLARDREGG
                     VFDLAPEELRIRDDYAEDRERFLSEGRSIFTQERLAQLASRWEHRTGKGSHDGRTLYG
                     ERYLEIRYEDLLVDTPSVFGGILEFLGAGSEDRLIERCLRSSSFERASSRRQGEEDSA
                     SFFRKGVAGDWRDVFTKRDRELYKDLAGERLVEFGYEKSYDW"
BASE COUNT          565 a          891 c          908 g          446 t
ORIGIN      
        1 acctgatccg gccagacttc gttatggccg acgcgacgcg caggggttgg atccactcca
       61 taaggaactt cgtgctcgac ggcgcgaaat acgcaagacc ccgcctcggc cccgaccatt
      121 acctcgtggt caaggagccg aacggctcgg tcggggctcc tctgctcatg gaagccctgc
      181 ccgagagccg catgatactc ctgatccggg acccaagaga cgtggtctca tcgacgctcg
      241 acggagccag aaagggaaac tggctctacg agcgcaagga caagggcgga tggaagcgcg
      301 attcgctgcc ggatgaaaac cccgacgcgt tcaccaggcg ctgggcgaac gtctacatgc
      361 aacacgcggg taacgccaag atggcctacg acgcccacag aggtccgaaa gtcatggtcc
      421 gctacgagga cctcagggca gacactttgg gcaccatgcg acgcatctac tcgacgctcg
      481 gtatacctgt agacggacat gagctggcac gggctgtgga gaagcactcc tgggagaaca
      541 tcccggagtg gaagaagggt gggggcaagt tttaccgcaa ggcaaccccg ggaggctggc
      601 aggaggacct caccccaagg cagataaagc agatcgagag gataacggcc cccctgctca
      661 aagagtatta ctcgggagaa gcgccgtaat attggctgag aaggtctcgg tcgtcatacc
      721 cactttcaat gcgggcccag gtttcgagga gctgctgcgc aagctcgacg cgcaagaggg
      781 aaatttcgag cgggaggtcg tcgtcgtcga ctcgggctcg accgacggca ccgcagagct
      841 cgccgcaagg cacggcgcga ttttgcacag cgtttcgaaa gccagtttcg accacggcgc
      901 gacgcgggac ctcgggatct cgctctcatc cggagaatac gtggccctta ctgttcaaga
      961 cgccgtgccg ctcgacgaaa ggtggctggc cgctttggtc gagaacctgc aaagtgacgg
     1021 ccggatcgcc gcggtctacg gccgccacgt cccgaggccc gacgccggcg tcatcaccag
     1081 ggcgctggtc ggcaaccttg ccgtctcaga caccgaaagg cgggagcagg agatccggga
     1141 cccagcgcac tacctgtcac tgccgccggc ccagaggcgc cgcatcgccg ccttcgacaa
     1201 cgtgagctcc tgcctgcgcc gccaggtctg ggaggagttc ccgttcggcg aggcagactt
     1261 cgccgaggac ctacgttggg gcaagaaggt ggtggaagca gggcacacca tcgtctacga
     1321 gccgcgctcg gtcatcgtcc actcccacga gcgcggtgcc ctctacgacc tcaggcgcca
     1381 ctacgtagac cagcgcgtcc tcgacgagct cttcggggcc cgcctggtgc cgaacctgcc
     1441 gcgcctgatc ctcggcatac cctacgcgac ccgccacgtc tatggcctcc tcaagcgcga
     1501 ggaaccggcg cctgaaaaga ggccaaagct cttgctcgac gcgatgacct acgccctcgc
     1561 ctcccagctc ggcgcctacc tggggggcaa gctcccttcc ctctcccgcc tgagcccgca
     1621 actccacgac aggctgcaca ccatcctcgg taagggagtg tgatcctcta tactctccgc
     1681 catgaacctc gaacgcgtcg tcgagcgcct gcgcgactac cggctgaaga acgagcggtt
     1741 cgaccggctg gtcggggctg tgctctccaa tgagaaggcc atgagagccg cgcgcgccac
     1801 cgtcgcagcg gctcgttccg tctcccgcaa ggggcagacc gtcgccgcag gggattatga
     1861 cacggtgttc ttcgtggcag gagagatgcg ctcagggacc tcctggttgc ggcacagcct
     1921 gagcgaccac cccgagatcg cctgcggcca agagggcagc ttcttcggcc gcggctacga
     1981 ccacgaggag atacccgtct acaccgggcc cgtctcgagc ctcacccgcg cccttgctct
     2041 ctccagggag ctgaagacct ggcacgcgct gccgtggaac ctgtggtcag acggctacga
     2101 cgaggacttg agaaacctct ctcggctctc ggtcgactac tttctctcca aagaggtggc
     2161 ccgcacggga cgcaggatag tgggggacaa gagccctcag cacaccgaga acctggacga
     2221 gatatacgag atctaccccg acgccaggat aatccacatc gtcagggacg gcagggatgt
     2281 ggcggtctcg gcgatgcacc actggtggcg gctggccagg gatagagagg ggggcgtctt
     2341 cgacctcgcg ccggaagagc tccgcatccg tgacgattac gccgaagaca gggagcggtt
     2401 cctgtccgag gggcgttcga tcttcacaca ggagcggctg gcccagctgg ccagtcggtg
     2461 ggagcacagg acagggaaag gcagccacga cgggaggacc ctctacggtg agagatacct
     2521 ggagatccgg tacgaagatc tcctcgtcga tacgccatcc gtcttcgggg ggattctgga
     2581 gttcctgggc gccggcagcg aggaccggct aatagagcgc tgcctgcggt cgagcagctt
     2641 cgagcgggcc tcgagccgca ggcagggcga agaggattcg gcctctttct tcaggaaggg
     2701 cgtcgccggc gactggaggg acgtcttcac gaagcgagac cgagagcttt acaaagatct
     2761 ggccggggaa cgattggtgg agttcgggta cgagaagtcg tatgactggt
//
LOCUS       DATMJA010000155         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_849127, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000155 DATMJA010000000
VERSION     DATMJA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849127"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VNT12_03635"
     CDS             <1..293
                     /locus_tag="VNT12_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45240.1"
                     /translation="LSETIRDLARAVETLSAYIEEPEHPVETRRFALEAAVGATAVLL
                     ERNDLRTSMLVGQIRSTAVDLLRASGMDSTESLEALRKAANRTTQEPTDPAI"
     gene            411..1247
                     /locus_tag="VNT12_03640"
     CDS             411..1247
                     /locus_tag="VNT12_03640"
                     /inference="COORDINATES: protein motif:HMM:NF009499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45241.1"
                     /translation="MSDARSTVSTNPASSSSVPATRDLRGRVALVTGVGRRRGIGSAV
                     CLTLASRGTDVVLSYWRAYDREMHWASDEDEPEALLRRLRAAGVRAEGIEMDLSRSDS
                     ARLLLDATEERLGRPSILVNTAAYSTRDGFETLDADAIDAHYAVNVRAMALLSVGFAR
                     RYPGGPGGRIVNFSSGQSLGPMPGELAYVATKGAIEAFTRTLAAEVGHKGITVNAINP
                     GPTDTGWMTEELKREMAPRFPSGRIGQPEDAAKLVAFLAGDEAAWITGQVIHSEGGFF
                     RY"
     gene            1288..1695
                     /locus_tag="VNT12_03645"
     CDS             1288..1695
                     /locus_tag="VNT12_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45242.1"
                     /translation="MKVPRKLPRLFREPPRTLFDDPHYEAYEAPVKGPLFSNAVFYAL
                     LAVFGIFLMLTADWFGLLLLNSEGVFRRIGPLNTVRVTAILMTGIAMPSALLMFHYKR
                     LYSYTSDENIFFAVTFSLSFFLGSPCVLTGIFG"
     gene            1815..>2614
                     /locus_tag="VNT12_03650"
     CDS             1815..>2614
                     /locus_tag="VNT12_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWI45243.1"
                     /translation="MADRLRFERSLSSPNEVFDTAGEPFSHYRPVLEEMGRMGLREWR
                     RRTHRAQRRRLEQQRELGMVPDDEIHPTDYVPRILPAADWAVLERGLAQRMLAINEWL
                     RRLEAGKDEVVPEEVIESSTLFDASIPTRFGDVPNRQMGFDIVAVERSGRTGEWEYLV
                     IEDNAKMPVGIEPMNLLRASTPDVLPESYTALLVRPLDGLMKRLGEVVRAASPRPDPT
                     LAILSTGPIDQYYLDHKLFASELGAVLAERHEVELDRGGRLVHKESGRR"
BASE COUNT          482 a          822 c          820 g          490 t
ORIGIN      
        1 cgctctccga gaccattcgc gacctcgccc gtgccgtcga aaccctctcg gcctacatcg
       61 aagaacctga gcatcccgtg gagacccgca ggttcgccct cgaagccgcg gtaggggcga
      121 ccgccgtact cttggagcgc aacgacctca ggaccagcat gctggtcggc cagatcaggt
      181 caaccgccgt ggatctcctg cgggcctcgg gcatggactc gacagagtcc ctcgaagcgc
      241 tccgcaaagc cgccaaccgt acaacccagg agcccacgga cccggccatc tgagccgggg
      301 agatgctcta gttccgatgc gggatctcgg cttgaggcgc gctagggggc gtgctacttt
      361 ccctacatcc gtcaatcccg cccggcgggc tctatgtcga ggagaagccc atgtccgacg
      421 ccagatccac ggtttccacc aacccggcca gctcttcttc agttccggct accagagatt
      481 tgcgaggtcg tgtggcgctg gtgaccggtg tggggaggag gagggggatt ggctctgcgg
      541 tctgcctgac gctcgcctcc cgaggcacgg acgtcgttct ctcctactgg agagcttacg
      601 accgcgagat gcactgggcg tcggacgagg atgaacctga ggcgttgctc aggcggctgc
      661 gtgccgccgg cgtcagggca gaggggatag agatggacct ctccagatcc gattcagcgc
      721 gactcctgct cgacgcgacc gaggagaggc tagggaggcc ctcgatcctc gtcaacaccg
      781 ccgcttactc cacgcgcgac ggcttcgaga cgctcgatgc agacgcgatt gacgcccact
      841 atgctgtcaa cgtgcgggcc atggcgctcc tgagcgtagg cttcgcaagg aggtatccag
      901 gcgggcctgg aggcaggata gtcaacttca gttcaggaca gtcgctaggg ccgatgccgg
      961 gagagctcgc ctacgtcgca accaaaggcg ccatcgaggc tttcaccaga accctcgctg
     1021 ccgaggtcgg gcacaagggg atcacagtga atgccatcaa ccctggtccg acggacacgg
     1081 gctggatgac cgaggagttg aagcgtgaga tggcaccgag gttcccttcg gggaggatcg
     1141 ggcagcccga ggacgcggcg aagctcgtgg catttttggc cggtgatgag gcggcctgga
     1201 tcacgggtca ggtcatccat tctgagggcg gcttcttccg ttattgagcg gcacgttcgc
     1261 cctctttgcg ctaccattac gtctcgcatg aaggttccca gaaagttacc caggctcttc
     1321 cgcgaaccgc cgaggacgct gttcgacgac ccgcactacg aagcctacga ggcgcccgtc
     1381 aaggggccgc tgttttcgaa cgccgtgttc tacgcgctcc tggccgtctt cggcatcttc
     1441 ctcatgctga ccgccgactg gttcgggctg ttgctcttga actcagaagg cgtcttccgg
     1501 aggatcggtc ctctcaacac cgtgcgcgtg acggccatct tgatgaccgg gatagctatg
     1561 ccctcggccc tcctgatgtt ccactacaaa cgcctctact cctatacctc tgacgagaac
     1621 atcttcttcg ccgtcacctt ctcgctctcc ttcttcctgg gctccccctg cgtgctcacg
     1681 gggatattcg ggtagctttt agcggtcggc catcagctat caggtaaagc tagcgagcat
     1741 catgcgaata cgctaccatc tgccgaatga agcttctttt cgcggcagga gcgacgtcct
     1801 caggcgggaa gctgatggct gaccgcttga gattcgagcg cagcctctcc tcccccaacg
     1861 aggtcttcga tactgctggc gagccttttt ctcactaccg gcccgtgcta gaagagatgg
     1921 ggcggatggg ccttagggaa tggaggcgga gaacccacag ggcacagagg aggaggctcg
     1981 agcagcagcg agagttgggc atggtccctg acgatgagat ccatccgacc gactacgtgc
     2041 cccgcatctt accggcggcg gactgggctg tgttggagcg gggactggcg cagcggatgc
     2101 tcgccatcaa cgagtggctc agacgtctcg aggctggaaa ggatgaggtc gtccctgagg
     2161 aagtcatcga gagcagcacc ctcttcgacg cttcgatccc aacccgattc ggggacgtcc
     2221 caaacaggca gatgggcttc gacatagtcg ccgtcgagcg ttccggccgg accggcgagt
     2281 gggaatacct cgtcatcgag gacaatgcca agatgccggt cggtatagag ccgatgaatc
     2341 tcctcagggc gagtactccc gatgtgctac ccgagtccta cacggcgctc ctggtgagac
     2401 cgctcgacgg gctaatgaag cgcttgggcg aggttgtgcg tgccgcttcg cccaggcccg
     2461 acccgacttt ggcgatcctc tccacggggc ctatcgacca gtactacctc gatcacaagc
     2521 tcttcgccag cgagctaggg gcggtcttgg ccgagcggca cgaggtcgag ctcgaccgcg
     2581 gcggaaggct cgtccacaaa gagagcggcc gccg
//
LOCUS       DATMJA010000156        10836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_316365, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000156 DATMJA010000000
VERSION     DATMJA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316365"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VNT12_03655"
     CDS             <1..181
                     /locus_tag="VNT12_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45244.1"
                     /translation="SDPDVVILRRQDGLFVAALSARGATKEGMIDASEEDRRELVQAH
                     TNPLSPEAAIVAPVI"
     gene            195..488
                     /locus_tag="VNT12_03660"
     CDS             195..488
                     /locus_tag="VNT12_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45245.1"
                     /translation="MLLMYGNDKVYAGYGDDLIYARDTDGVDYIDCGAGFDKVETIQR
                     DDETLSNCERTRRSRRSLRGAGSRKGGWTATHLGKGWRQRSLKEVLFLHLKAI"
     gene            complement(495..1205)
                     /locus_tag="VNT12_03665"
     CDS             complement(495..1205)
                     /locus_tag="VNT12_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HWI45246.1"
                     /translation="MRFVVASEVFELFPGLKLPVAVAEGVHPTTDVPGIETMWLQSWE
                     EAARTASDYQSPQSHPRVAPWREAMTAMGVSGRRFPSSIEALLRRAFKGGEPPRINPL
                     VDFYNAVSLRHVVPAGGFDLEEIDGALELRLTRREDTFQPLDGPSDVSVEPGEVAYAS
                     GNQILTRYFVWKQSRKGLLDESTRSLFLVSEVLGEVEVDSGVADAVLEDFADGLRRYF
                     DSEPAVFLVGEEDPEISP"
     gene            1538..2356
                     /locus_tag="VNT12_03670"
     CDS             1538..2356
                     /locus_tag="VNT12_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI45247.1"
                     /translation="MLRQGTVEGNVGITDAGYGIRTGRSREIFGRLERSIPGGNTRSL
                     AYFPPYPLVISSGSGCRIRDADGNEFIDLLNNYTASVHGHALAAINEAMSNQAVLGTV
                     FPAPSELQAELAERIVGRVASVEKVRFANSGTEAVMMAVRGARAFTEREKIIKAEGGY
                     NGMWEQVPISWSQDPYRAAMPEGVRELVRMVDYNDVGQLEAAMEEEGDEVAAIILEPV
                     TGTGVFAGTPRYFAATPGRRARGAPHLRRGHNPQARRRRLPGGLRRSSRPDDDG"
     gene            2283..2858
                     /locus_tag="VNT12_03675"
     CDS             2283..2858
                     /locus_tag="VNT12_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI45248.1"
                     /translation="MTLRLGVGGFQEVLGVHPDLTTMGKIIGGGLPVGAFGGRADVME
                     VFDPRSPDHLHHSGTFNGNLMTMAAGCVALDLLTRGEIARINALGERLAEGLRRSLAE
                     RPDLDGVVNNCGSLLHVNFGTEVRKFSDLKLDSPMAAAFHLAALDEGVYFAPRGFMNT
                     STAMDEQVVDEVLEACSRAIETLPVPLEPET"
     gene            3164..3565
                     /locus_tag="VNT12_03680"
     CDS             3164..3565
                     /locus_tag="VNT12_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI45249.1"
                     /translation="MSGALARAGLRPPERFFLSYPHELSGGQRQRVVIAAAVALEPRV
                     LVADEPVSMLDASVRGEILELLVRLKDEVGLTLVLITHDLAFAWALADRVAIMYLGKI
                     VELGPAQTVLTEPLHPYTKSLRSVVAGPTRF"
     gene            complement(3849..5009)
                     /locus_tag="VNT12_03685"
     CDS             complement(3849..5009)
                     /locus_tag="VNT12_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45250.1"
                     /translation="MVLTLGLAVVLLWLLAGCTVADQRHRAEDPNGRLRASSAGSSGG
                     DQHAEISNGKIAFIRHSDINVMNSDGTGQTNLTRTKAGVGGGVWSPNGKKIAFSARST
                     GERYLDIYVINASGTGETNLTRTKASSEGAPSWSPDSKRITYLRGSDPSGEIYNDINV
                     MNSDGSSQTRLIKATDTKNFEVGFESPAWSPDGEKIAFMRTTRVDFVNSPPSSAVPAT
                     GPSGLYLMKPDGTGLRKLSKEMAYAQSPLWSPEGKEIAFSGIGEKKYVVNVGGRELRE
                     LMPNIRDHISSYSWSPNGKKIAFAAVHYRGELDIYVIDADGTGQTNLTSTKTIIEDEP
                     SWSPDGKQIAFTRADAGDVYADQDVYLMNADGTGRLCLANNASSPSFAPRDR"
     gene            complement(5032..6549)
                     /locus_tag="VNT12_03690"
     CDS             complement(5032..6549)
                     /locus_tag="VNT12_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWI45251.1"
                     /translation="MALAAEVGQKPNILLILTDDADFSLLPKMPQIKEQLVRKGTTFP
                     NAFAPFATCCPSRASILRGQYPHNTGVISNYGTHGGVDAFEAAGNDEDNLATRLDGAG
                     YSTGLFGKYLNEYGGGYVPPGWHEWHGWAGSYASGKIYENDRLNAYDLSRRHESDILG
                     SKARSFVRNAKGSPWFAYVAFNSPHNPTYTESEYSDNYRDQGSPKSPAFDEADVSDKP
                     DWVRQLPRISDQDRATYNRRYRNRLRAMRSVDDAIAGLLRSLRETGQIDNTYIVLWND
                     NGYHMGQHRIPEGKRTAYEEDVRYPMVVRGPGVVHDANDERLVSGTDLMPTFLELADA
                     PTPEYVDRRSLVPLLSGSKLPWRDSLLLEGYDDGFGDKTYVPPDFKAIRTSDERTYVE
                     YEIGERELYDLRTDPRQLKSLHDEPERAVEVAALSARLQALKYCSGKGCRCAEQTATA
                     RPRCSLYPSWGGLSHAIPLSFIPLSVVPGEALLNVSVGGASGTGVFTDVSAKERD"
     gene            complement(7081..7740)
                     /locus_tag="VNT12_03695"
     CDS             complement(7081..7740)
                     /locus_tag="VNT12_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI45252.1"
                     /translation="MEDTVRVLIAEDHPLFREGMRGRLDRVADVAVVGEAASGEEAVE
                     LANKLEPDVILMDIKMPGLNGIEATREIQQANPLIGVLVLTMFEDDDSVFAAMRAGAK
                     GYLLKDSGGEGVVHAIRAVASGEAVFGPGVAERIMGFFSAPRAAAPQRAFPELTEREE
                     EVLSLVAQGKGNREIARQLFVSLKTVRNHVSNILLKLQVADRAQAVIRARDAGMGRDR
                     M"
     gene            complement(7743..9587)
                     /locus_tag="VNT12_03700"
     CDS             complement(7743..9587)
                     /locus_tag="VNT12_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HWI45253.1"
                     /translation="MGRFNAGRMGLRSGLIRIGRHLRRGAAAYGVLLIALLLTLLAFY
                     YVRQNVEAEARTRFDETTRATRAAIYRRVNAYLAAMFGARGLLRVSDSVERDEWDVYV
                     RSIEVGRRLRKAQSLEGLQSLGFATYLRSGERGAYSRVANEEGVRDLWPEPDGESSAY
                     FPLKFVGPSYEANRKMINYDAYSDPTHRSVMDRARDTGEPQATGMDYVLTNAPSHSEA
                     DLALRRGFVVYLPVYQEGEPKGTVAERRRALRGFIVGTFKAEELFAHTFGRTFHPAID
                     FEVYDGEDVASSSLLYDSHSVRSAGEKGDATLFSKVGRIKVADTRSGAREWALYFATL
                     PGFERGAKSNLPAFVLASGVTVSLLLFGITLMLARSRTQAFRASADLKEANLELEQAN
                     TDLQRSRENLINTREEERRRLRRDLHDGVGPQLAALMLELETASDLVSDNHEASALIA
                     KLSKRARGIVSDVRRSVHALRPPALDELGLVEALREGALQYGPADLRVSVENPEELSH
                     LPAAVEVACYRIAQEALANVVRHARASHCSIRIRLDEDAGALSVEVEDDGRGIRDDDR
                     AGVGMISMRERTEELGGWCTVESVVGGGTLVRALLPFQIKEDTPRREE"
     gene            10159..10458
                     /locus_tag="VNT12_03705"
     CDS             10159..10458
                     /locus_tag="VNT12_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45254.1"
                     /translation="MNTPTSISEQAQRRIGAAGIRGGMMRRVRGEILLLAIILLFLTV
                     GVGVRSIRDISAAETRVAPTGIVAPTTATLSPDPNSDGGNEDVAQVTSRARFKAH"
     gene            complement(10627..>10836)
                     /locus_tag="VNT12_03710"
     CDS             complement(10627..>10836)
                     /locus_tag="VNT12_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI45255.1"
                     /translation="RVIVVATDDILAFYEDLLAKGVYFELRPTESLNGTQAMFRDPDG
                     NAIVLWERSSFALREGTSWFGRRET"
BASE COUNT         2012 a         3354 c         3278 g         2192 t
ORIGIN      
        1 gtctgatcca gacgtagtga tcctgcgccg ccaggacggc ttgttcgtgg cagctttaag
       61 cgccagaggt gccaccaagg agggcatgat cgatgcctct gaggaggatc gtcgggagct
      121 tgtccaggct cacactaacc cgcttagtcc cgaagctgcg atcgttgcgc ccgtgatata
      181 ggggaccgcc aaccatgctg ctgatgtatg gcaacgacaa ggtgtatgct ggctacggcg
      241 acgaccttat ctacgcgcgc gacaccgacg gtgtggatta catagattgc ggggctggct
      301 tcgacaaggt cgagaccata caacgcgacg acgagaccct gagtaactgc gagagaaccc
      361 gcagatcgag gcgttcgctg cggggagccg gttcgcggaa gggtggctgg acggctacgc
      421 atctcggaaa gggctggcgg cagcggtctc tcaaggaggt gttgttcctc cacttgaagg
      481 caatctagcg ccagctatgg agagatctcc ggatcttctt ctccaacgag gaagaccgcc
      541 ggctccgagt cgaagtagcg acggagaccg tctgcgaagt cttccagtac ggcgtccgct
      601 acaccgctgt cgacctcgac ttccccaagc acttctgaga ccaggaacag ggatctggtg
      661 gactcgtcca ggagtccctt gcgggactgc ttccaaacga agtagcgggt cagaatctgg
      721 ttaccggagg cgtaagccac ctcgccaggc tcgacgctta cgtcggaagg cccgtccagc
      781 ggttgaaagg tgtcttcccg gcgggtgagc ctcaactcga gcgccccgtc tatttcttcc
      841 agatcgaaac ctccggcagg gacgacgtgc cgcaaagaga cggcgttgta gaagtccacc
      901 agcgggttga tgcgcggagg ctcgccgccc ttgaaagctc gtcgcagtag agcttctatg
      961 gagctgggga atctcctacc agagactccc attgcggtca tggcctcgcg ccagggagcg
     1021 actcggggat gtgactgtgg gctctggtaa tccgacgctg ttcgtgccgc ctcctcccag
     1081 gactgtagcc acattgtctc gatacctggt acgtcagtgg tggggtgaac cccttccgcc
     1141 acggcaactg ggagcttcaa gccaggaaac agttcgaaga cctccgaagc tactacaaac
     1201 ctcatgatgt acctgccttt acgtgtgcct tttttcagac gcggcttcca ctgcacctcg
     1261 cttactaccg ccttcggagc ctacgtcatc ggagacactt cgggtctccc ttccatcaac
     1321 gacgtatacg ccagtcaggg taacagtcgt ttcgaccaac gacagctgcg tcgagaaact
     1381 acccgagatc tagcgcaccc ttgccatgaa gcctcttctg gagcgagggc ttgtggagtg
     1441 agggacggcg tagctcagcc gaccgctaga cgggacgagg atgcagtaga atataattct
     1501 tgcgagaacg attagctgtc tatgaacgta tgtacagatg ttgcggcagg ggacggtgga
     1561 gggcaacgtg ggtataacgg acgccggata cggtatcagg accggaaggt cgcgtgagat
     1621 ctttgggcgg ctcgagcgtt cgatccccgg cggcaacacg cgctccctcg cctatttccc
     1681 tccttatcct ctggtgatct cctccggctc cggctgtcgc atcagggacg cggacggcaa
     1741 cgagttcatc gacctcttga acaattacac cgcctcggtc cacggtcacg ccctggctgc
     1801 cataaacgag gccatgagca accaggcggt cctggggacc gttttccctg ctccaagcga
     1861 gctgcaggca gagctcgcgg aacgaatcgt cggccgggtg gcgtcagtag agaaggtgcg
     1921 cttcgcgaac tcaggtaccg aagccgtcat gatggccgtg cgcggtgcca gagcctttac
     1981 cgaaagggag aagataatca aggcggaggg cggctacaac ggtatgtggg agcaggtgcc
     2041 gatctcctgg tcgcaggacc cgtacagagc tgcaatgccc gagggggttc gcgagctggt
     2101 gcgcatggtc gactacaacg acgttggcca gctcgaggcc gcgatggaag aggaaggcga
     2161 cgaggtggct gcgataatcc tggagccggt aacggggacg ggggtcttcg ccgggacccc
     2221 tcgatacttc gcggcgacgc ctggccgacg agcacggggc gctcctcatc tgcgacgagg
     2281 tcataaccct caggctcggc gtcggcggct tccaggaggt cttaggcgtt catcccgacc
     2341 tgacgacgat gggtaagatc atcggcggcg gcctccctgt cggtgctttc ggcggacgcg
     2401 cagatgtgat ggaagtcttc gacccgcgct ctccagacca cctccaccac tcaggaacct
     2461 tcaacggcaa cctcatgacc atggccgcag gctgcgtagc actggacctc cttacgcgcg
     2521 gggagattgc acgcatcaac gccctgggtg agcggctcgc cgaggggctg cgccgctctt
     2581 tggccgagag gccagatctc gacggcgtcg tcaacaactg cgggtctcta ctgcacgtga
     2641 acttcggaac cgaagtccgc aagttctcag acttgaaact agacagcccc atggcggccg
     2701 ccttccatct cgcggcactc gacgaaggag tctacttcgc gccgcgcggc ttcatgaaca
     2761 cgtctaccgc gatggacgag caggtagtag acgaagtgct cgaagcctgc tcccgggcca
     2821 tcgaaacgct ccctgttccc ctggagcctg aaacctgaag ccgtgtcgtg cggaaggatt
     2881 tgacctttcg cacgacaatt tgagcgtaac cctgcttgaa cgcggatcag gaaaactcta
     2941 ctattgacca acagtcaccc tggggcagga agtaggggag aatgcgaagg atcgacgctg
     3001 ccgggacact cctgttatgt cctgatcgtg tctctccaga gcacattgcc tctgtgtagc
     3061 gagagatcct catcgtgaac ggtggcggtc ctaacgtccc ctggactccg gtgtgggacc
     3121 ggatcgacaa ggatttcgat gagcagggga cgaaggggag atcgtgtcgg gcgccctggc
     3181 gagggctggc ctgagaccac ccgagcgctt cttcctgagc tacccgcacg agctatctgg
     3241 gggacagcgc cagcgggtgg tcatagcggc tgcggtagcg ctcgagccca gggtgctcgt
     3301 cgccgacgag cccgtctcca tgctcgacgc ctcggtgcga ggcgagatcc tcgagctcct
     3361 cgtccggctc aaggacgagg tcgggttgac cctggtcctt atcacccacg acctcgcctt
     3421 cgcctgggcg ctagccgacc gggtcgcgat catgtatctg ggcaagatcg tcgagcttgg
     3481 gccggcgcag acggtactca ccgagccctt gcatccctac acgaaatccc tgaggtcggt
     3541 cgtcgccgga cccacaaggt tttgacaggc gagcctccag atccagccca catccccctg
     3601 ggctgccgct tcaacccccg ctgtccggtg ctgcgctcgg gagaggcagg ctcccaagaa
     3661 ccccgctgcc tgggagaggt cccggccctc gaggagcctg tgcccggcca tagagcagcc
     3721 tgccacgtgg gcgcggccag aagcgtccga acatcctgat cccccacggc gctacgggcc
     3781 cactcgggcc gtttcgcgca accctgagtg cttcaggttc gtgcgcgctt gacgcctcat
     3841 cgtttcaact acctatcccg aggcgcgaaa gaagggctac tagcgttgtt ggcgagacac
     3901 aatcgtccgg ttccgtcggc gttcatcaag tagacatcct ggtcggcata aacatcgcct
     3961 gcgtccgctc gtgtgaaggc tatctgcttg ccatccggcg accaggaggg ttcgtcttct
     4021 ataatggtct tggtactggt gaggttggtc tggccggtgc cgtcagcatc gatcacatag
     4081 atatcgagct ccccgcggta atgtactgct gcgaacgcta ttttcttgcc attgggtgac
     4141 caggagtacg aagagatgtg atcccgtata ttcggcatta gctcccttag ctccctgccg
     4201 ccgacgttca cgacgtattt cttctcgcca atcccactga aggctatctc cttgccttcg
     4261 ggagaccaga ggggggattg agcgtaagcc atctccttgg acagcttcct tagacctgtg
     4321 ccgtcaggtt tcatcaggta gaggccactc ggtccggtgg caggtaccgc tgaggaagga
     4381 gggctattga caaagtctac cctggttgtc ctcatgaagg ctatcttctc gccatcggga
     4441 gaccatgccg gcgattcgaa gccgacttca aagttctttg tgtccgtggc cttgatgagc
     4501 cttgtctgac tactgccgtc ggagttcatc acgttgatgt cgttgtaaat ctcgccgctg
     4561 gggtcgctgc ctctcagata ggttatcctc ttgctgtcgg gcgaccacga gggggcacct
     4621 tccgagctcg ccttcgtcct ggtcaggttg gtctctccag tgccggaggc attgatcacg
     4681 tagatatcga ggtagcgctc cccggtggac ctggcggaga aggctatttt cttgccattc
     4741 ggagaccaca cccctccgcc tacacccgcc ttcgttctgg tgaggttcgt ctggccggtg
     4801 ccgtcggagt tcatcacgtt gatgtcagag tgccttatga aggctatctt gccgttcgaa
     4861 atctcggcat gctggtcgcc cccgctgcta ccggcggacg aggcccgcag cctaccattg
     4921 gggtcttcgg ctctgtgccg ctggtcggca actgtgcacc ctgcgagcaa ccaaagcagt
     4981 acaacggcta agcctaatgt gagcaccaag gtcgttcgcc gtgtcatcgc ttcaatcccg
     5041 ctccttggca ctaacgtcgg taaatacccc ggtccccgag gcacccccga cgcttacatt
     5101 cagcagcgcc tcgcctggta cgacgctcag cggtatgaag ctgagcggta ttgcgtgaga
     5161 aaggccgccc caggaaggat agagagaaca gcgcgggcgg gcggtcgcgg tttgttcggc
     5221 gcaccggcac cctttaccag agcaatactt cagggcttgt aagcgggccg aaagcgctgc
     5281 tacctctacc gcgcgttcgg gctcatcgtg taggctcttg agctggcgtg ggtcggtcct
     5341 caggtcgtac agctccctct cgccgatttc gtactcgacg taggtgcgct cgtcggaagt
     5401 ccttatcgcc ttgaagtcgg gcgggacgta ggtcttgtcc ccgaagccat cgtcgtagcc
     5461 ctccaaaagt agcgagtcgc gccagggaag tttcgacccg gagagcaggg gcacgaggga
     5521 tcggcggtcc acatactccg gcgtcggggc gtccgcaagc tcgaggaagg tgggcatcag
     5581 gtctgtgccg ctgaccagcc tctcgtcgtt cgcgtcgtgc accacgccgg gaccacggac
     5641 gaccatcggg tagcgcacgt cctcctcgta agcggtcctc ttgccctccg gtatcctgtg
     5701 ctggcccatg tggtagccgt tgtcattcca gaggactatg taagtgttgt cgatctgccc
     5761 cgtctccctc aggctccgca gcagccccgc gatggcgtcg tcgacgctgc gcattgccct
     5821 cagcctgttg cggtacctgc ggttatacgt cgccctgtct tggtcgctta tgcgcgggag
     5881 ctggcgcacc caatcgggct tgtcggacac gtccgcctcg tcgaacgccg gggatttagg
     5941 ggaaccctgg tccctgtagt tgtcggagta ctccgactcg gtgtatgtgg gattgtgggg
     6001 gctgttgaag gcgacgtagg cgaaccacgg agagcccttc gcgttcctca cgaacgacct
     6061 ggccttcgag cccaggatat ctgactcgtg gcggcgcgag aggtcgtagg cgtttagcct
     6121 gtcgttttcg tatatcttcc cgctagcgta ggatccggcc cagccgtgcc actcgtgcca
     6181 gccgggtggg acgtagccac cgccgtactc gttcaagtac ttgccgaaca gcccggtgct
     6241 gtaaccggcg ccgtcgaggc gggtggcgag gttatcctcg tcgttgcctg ccgcttcgaa
     6301 cgcgtcgacg ccgccgtggg tgccgtagtt ggagatgacg ccggtattgt gcgggtactg
     6361 cccgcgcaag atgctcgcac gcgaggggca gcatgtggcg aagggagcga aggcgttggg
     6421 gaaggtcgta cccttgcgga ccagctgttc tttgatctgc ggcatcttgg gaagcaggga
     6481 gaagtcggcg tcgtcggtga ggataagcaa aatattcggc ttctgcccca cttccgccgc
     6541 cagtgccatg ttgctcctgc cgttcggctc cccgaaacac gcaacggccg caaccaagca
     6601 aaatacgacc accactgcta accggagtgt gttcgtagtc gccatgacta ctcgcgtccc
     6661 ttcttgaagc gaacgccaga tccgcttcgc tattcgccag cggtctcgct gagcttgtgg
     6721 cgaccatcgt agaggcgcgg tgttatctgg gcgttaactg gagcgcctca aggcatggtc
     6781 agaattgagc attttcgact acgccttggt ccagaggagt caaaacctca aggagaggta
     6841 ggacctatcg caggggatta tgaggaagcc agggaagctg cgatcatgat cgaagaaggc
     6901 aggactgagt tcggcatgcc cataggcgcc gaatcggggc tatgcgagcg ggcctcatcc
     6961 aaactggttc atctgagtga tgtgcctacc ccgacagagg aggaacctac aatcggtata
     7021 cggggttccc tggcagtttg acgacggcgt acgttcgagt ggccgatgcc cccagcatcc
     7081 tcacatccta tcgcgtccca tcccggcgtc gcgggcgcgg ataacagcct gcgccctatc
     7141 ggccacctgc agcttgagca ggatgttgga gacgtggttt ctaacagtct tcaggctaac
     7201 gaacagctgc ctggctatct cccggttgcc cttgccttgc gcaaccaggg agaggacttc
     7261 ctcttcgcgc tcggtaagtt ccgggaaggc gcgctgaggt gccgctgccc tgggtgcaga
     7321 gaagaagccc ataatgcgct ccgccacccc gggcccgaag accgcttctc cgcttgccac
     7381 cgctctgatg gcgtgcacta ccccttcgcc gccagagtcc ttcaagaggt agcctttggc
     7441 cccggcgcgc atcgcagcga agaccgaatc gtcgtcctcg aacatggtga gcaccaatac
     7501 gccaatcagc ggatttgcct gctggatctc gcgggtggcc tctataccat tcaggcctgg
     7561 catcttgatg tccatgagga tgacgtcggg ctcgagcttg ttggccaact ccaccgcctc
     7621 ttcgccgctc gcggcctctc ccacgaccgc gacgtcggcc acccggtcca ggcggccgcg
     7681 cataccctcg cgaaagagag ggtggtcctc ggctatcagc acacgaacgg tatcctccat
     7741 gctcactcct ctctgcgggg cgtgtcttcc ttgatctgga aaggcagaag cgccctgacc
     7801 agggtgccgc cccctaccac ggactctaca gtacaccagc ctccaagctc ctcggtcctc
     7861 tcgcgcatcg agatcatccc cacccctgcc ctatcgtcat ccctgatgcc cctcccgtcg
     7921 tcttcgacct caacgctcag ggctcccgcg tcctcgtcca gccgtatgcg aatcgaacag
     7981 tggctcgctc tggcgtgacg caccacgttg gccagggcct cctgggcaat cctgtagcat
     8041 gccacctcca ccgccgcagg caagtgcgat agctcctctg gattttccac cgacacacgc
     8101 aagtccgctg gaccgtattg cagcgctcct tccctcaggg cctcgacgag gccgagctca
     8161 tcgagggcgg gcggcctcag ggcgtggacc gagcgcctga cgtccgagac tatccccctg
     8221 gcgcgcttgg agagtttcgc tatcagggcg gaggcttcat ggttgtccga aacgagatcg
     8281 cttgctgttt cgagctcgag catgagcgcg gctaactgag ggcccactcc atcgtgcagg
     8341 tcccgtctca ggcgccgcct ctcctcctct cgagtgttga tcaggttttc tctcgacctc
     8401 tgcagatctg tgtttgcctg ctccagttct aggttggctt ccttgaggtc cgcgcttgcg
     8461 cggaaggctt gcgtacggct gcgcgcgagc atcaaggtga tgccgaagag caagagactc
     8521 acggtgacac cgctagcgag aacgaaggcg ggtaggttgc tcttggctcc tctctcgaat
     8581 cctggcagcg tggcgaagta aagggcccac tcgcgtgccc cggatcgtgt atcggctact
     8641 ttgattcgtc ccaccttgga gaagagagtc gcatccccct tctccccggc gctcctcacg
     8701 ctatggctgt cgtagagcag cgagctcgat gccacgtcct cgccgtcgta gacctcgaag
     8761 tctatggcgg ggtggaatgt tctgccgaag gtgtgggcga agagttcttc cgccttgaag
     8821 gtaccgacga tgaatcctct cagggcgcgc cgccgttcgg caacggtacc cttgggctcg
     8881 ccctcctggt aaaccggcag atagaccaca aaacctctcc tcaaggccag gtctgcctcc
     8941 gagtgtgacg gagcattggt caatacgtag tccatccccg ttgcctgcgg ctctccggtg
     9001 tctcgcgccc ggtccatgac ggatcggtgg gtgggatccg aatacgcatc gtagttgatc
     9061 atcttccggt tggcctcgta ggaaggtccc acgaacttca gggggaagta agccgagctt
     9121 tcgccatccg gttctggcca caggtcccgc acaccctctt cattggcaac tcgggagtat
     9181 gcccctctct ccccagatct taagtacgtc gcaaagccca aagactgaag cccctcgagg
     9241 ctttgcgctt tgcgaagtcg gcgccccact tctatgctcc tcacgtagac atcccactcg
     9301 tctcgctcca ccgaatcgct cacacgcaac aacccccggg cgccgaacat cgcggcgaga
     9361 taggcgttaa cgcgacgata aatggctgcc ctcgtcgccc gggtggtctc gtcgaaccgg
     9421 gtgcgtgctt ctgcctcgac gttctgacgg acgtagtaga aggcgaggag cgtgaggagg
     9481 agcgctatca agagtacccc gtatgccgct gccccacggc gcaggtgacg tccgatgcgg
     9541 atgagccccg accgcagtcc catccggcca gcgttgaacc tacccacatc aaacaccacc
     9601 tacgaccgaa gatgccctgg cggcgagcag ccagcggtta agcaccgcgg tggacgacca
     9661 cgcgatccta ctctcgtagg acccccccac atcgggttgt gacctcatcg gtggttcgcc
     9721 ttcctactca atccctttct caacctgcaa tgatggcacc agacgcagtc cggtgcatct
     9781 cgagcggccg accttcgaga gcccgtcggt gcggtgggta gcagcgccgt ctcatgtgcg
     9841 gcctctgccg caagcctaga cgggaaggtg tcccgccgtc atgggcacga ttcccgagtt
     9901 ggacgggaat ctcttcccga gcagctcggg atagatgccc gagggggtcc gctactgaag
     9961 cctgtgggac caggcgggac gccttcccgc catcgtcgac gcgactcccg cgtcggatgg
    10021 gattatcttc ccgaacactt tgggatgggt gctcatgacg gcgggatatt tccctcctta
    10081 ggcttctctt cgtaatagca atgcgctctc tggtgaggga tctcctctcc accggagaag
    10141 ctaaggaaag gagaggacat gaacaccccg acctccatca gcgagcaagc ccaacggcga
    10201 atcggcgctg cgggaataag aggaggaatg atgaggagag ttaggggcga gatcttgttg
    10261 ttggccataa tcctgctgtt cttgaccgtg ggagtcggag taaggtcgat tcgcgatatc
    10321 agtgcagcag agacgagggt cgcgccgacc gggatcgtcg cgccaaccac cgccacgctt
    10381 tcgcctgatc cgaactcgga cggagggaac gaagacgtgg cgcaggtgac ctcgagagca
    10441 cgcttcaaag ctcactgaat ctcttggtct tccttggcgg cctcgatgac tccctccctg
    10501 gcacacttct agtgctgaag gctgccagga gggagtcgtc ctgatgacgc aggatcactg
    10561 tttccgcgtc cggctcgtcc cgatggcggt tcaacaaggg caatgggagc tcttacgtct
    10621 gcaaagctat gtttcacgtc gcccgaacca cgacgtgccc tctctcaagg cgaacgacga
    10681 acgctcccag agcacgatgg cattgccatc agggtcgcgg aacatcgctt gcgtaccatt
    10741 gagcgactcc gtaggccgta actcgaagta gacgcccttg gcgagaaggt cttcgtagaa
    10801 cgctaggatg tcgtcagtgg ctacgacgat gacccg
//
LOCUS       DATMJA010000157         7520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_612352, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000157 DATMJA010000000
VERSION     DATMJA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612352"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VNT12_03715"
     CDS             <1..846
                     /locus_tag="VNT12_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI45256.1"
                     /translation="LQEVLAKTRYSGPVTDSEAKAGDRIIEIRAAPLEDGALAILRDV
                     TEERHMQRVKAEFIANASHELKTPLTALSGYLEMLEDEEDERVRAEFLNDMRAQTGRL
                     QSLARTLLDLSRLDANAVTFRTEEVDLEDLLHGLRRDFGYTGRSLNIRAEEDVPPVKT
                     DPNQLHRAIAILVDNALKYSDEELPVDLGLSLQDGRVVISVTDRGCGIPESEIPHIFD
                     RFYRAQGSSRADGTGLGLALAHEITDHLGGDIQVHSRPNAGSIFSVLLPLAADPHGKN
                     GSRSP"
     gene            complement(1011..1652)
                     /locus_tag="VNT12_03720"
     CDS             complement(1011..1652)
                     /locus_tag="VNT12_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWI45257.1"
                     /translation="MFLRFFEADPSAAVAFLLGLVIGITVHEASHATSAYLLGDDTAY
                     RDGRVTLNPISHLDLLGSMMLLLAGFGWGRPTPVVPSKLRGGVFGAVAVALAGPISNL
                     LIVAVCAALYLLPPFREPGGYLFILVVMVAFTNALLFVFNLIPIPPLDGSKVIFPFLP
                     RALDGFVDFMNQYGPMILLGLILVSFVTRLPILSILLAPVRPLLTLLGLPVVF"
     gene            complement(1934..2599)
                     /locus_tag="VNT12_03725"
     CDS             complement(1934..2599)
                     /locus_tag="VNT12_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45258.1"
                     /translation="MAVSLLVLGAVALVFAVFVQTKAADMAERAVPIDPNNAGPATVL
                     AEASGVSISTPIRPASLTGLGYHPEGESLAAMEPRGKNLSANVVLGLLSGGETPEKIH
                     YYVMDAAGRGGPQTGAVDVGAATGTTVYAPVAGTVTAIRPDPMVQNANVIEIKPDANP
                     DVRLSVALVLSDGEAGVNDSVTAGKTVLGTVADSAEVLDPQLSSYTSDAGNHVTVTVS
                     KSG"
     gene            2889..4058
                     /locus_tag="VNT12_03730"
     CDS             2889..4058
                     /locus_tag="VNT12_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWI45259.1"
                     /translation="MSARKLVVSTYLQYIVLAVGILLLVSFVRQVSGVLLTFLAAGVL
                     AYTLNPVVRRLEGLRVPRALAVIGVFLVLIFVVIVTLLLIIVPSVGQVQIVVRNPQDL
                     VDQVIALLNRIQNVPYVGRYVAELDQDRILQLLKTNAPSAEQVANVATGVIGGVFGVF
                     GTLFNLLLMVLVSVYLLLEREKITRALLNTIPETVRDQSLELFYAVEHTLVRYLRGQI
                     FLCAIMGLIGAGIMYFTVGKYALPVGAWVAATEIIPVIGAFLGAVPAVGIALFVPQGG
                     FVQALIVAGLFLVAQQLEGNVLVPRIQGGSVGVHPLWVLFATLAGTALYGLIGAVFAV
                     PVVAIIAATLRYLRGTLIFERWGRPPVKPAEEPETGTPAAKLGAGPHGDRKGDDE"
     gene            4055..4666
                     /locus_tag="VNT12_03735"
     CDS             4055..4666
                     /locus_tag="VNT12_03735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWI45260.1"
                     /translation="MTIPHREPQKPSFREASKDALLLVLHPLVRWLSIMRIRPDTLTV
                     IGWTLALFSAVLFGLGYARIAGAVMLLGGLFDALDGAVARESNTMSSFGAFLDSTLDR
                     LSESAVFVGLIFFYASSARPYEALLAAMAMTFSLLTSYARARAEGLNIDCRVGLLERA
                     GRVVILSVFSIVGLPTVGLGLLAAGALVTTAQRILHVRRATRG"
     gene            4650..5168
                     /locus_tag="VNT12_03740"
     CDS             4650..5168
                     /locus_tag="VNT12_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45261.1"
                     /translation="MRPADKETSRTRDAVGDYTRKTEYEVSRLSNRMKEGGHPAPLGD
                     PTSGVVLVLEQPFGPRTLEALELSLQALRLQQAYVTYESTGLLAQEIQTTEPTVLVAI
                     GAGAARNIDAIDYPLVRQPFSEAEPGVWFSWTKGTKGLLTPALAPALDDDAAKRRFWS
                     AFLTLRDLANNP"
     gene            5473..7224
                     /locus_tag="VNT12_03745"
     CDS             5473..7224
                     /locus_tag="VNT12_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWI45262.1"
                     /translation="MFLGLAIALVFVTGISAGAEPGGSIRSKKADVAAVQDRLVEIRL
                     KASSAEAAYNNALYEMNQLNGQITRTTTDLRAAKERLAVAKETLEDRAAQVYKSGNVA
                     FMDVLVGVDNFSEFASRLNLWMRLLARERAEFLEVREARNELAARRDRLEAQREQRVA
                     AVETAITQRGRANEAEAQAEAYLNSLNAELRDAIQAAQDRQATQARAAAAAMVENTPE
                     PVEEPEPIPEVQVSQVKQARVPRADLQSERAAARREDAAQAAAERAERLAEKRAAERE
                     AARKAAEQAAEQAAAERQAARRAAEQRAARREAHRQAELAARRAAKRQAAREEAEHQA
                     ELAARRAEIEEAAAEQAAAERKAARLAAKRRAERRAEERAAASASASAAAPEKAAAER
                     RADRRDERRAEERAEASASASASASASAPSSNNSRRGGGGDRGGSAGASSASATASAS
                     ASGSRGGGGVSASGDEVLAVADDHLGTPYVFSPPGPCVAFEAEDCSCFTMLVFQEFGI
                     ALPDSPGGQMGYGTPVHGAPQAGDLLFWSEDGSGYITHVGIAMGDGTTIHAQVHNEVT
                     ITSINYVEGYVGARRLL"
BASE COUNT         1431 a         2332 c         2422 g         1335 t
ORIGIN      
        1 ctgcaggagg tactcgccaa gacccgctac agcggtcccg tgacggattc cgaggcgaag
       61 gcaggggaca ggatcatcga gatccgggcc gcccccctcg aggacggggc gctcgccatc
      121 ctgcgcgacg tcacggagga gcgccacatg cagcgcgtca aggcagagtt catcgccaac
      181 gcttcccacg agctcaagac gcctctgaca gccctctccg gctacctgga gatgctcgag
      241 gacgaggagg atgaacgggt cagggcggag ttcctgaacg acatgcgagc ccagaccggg
      301 cgcctgcaga gcctggcacg taccctgctc gacctctcgc gcctcgacgc caacgccgtc
      361 accttcagga cggaggaggt cgacctcgaa gacctgctcc acgggctgcg cagggacttc
      421 ggctatacgg gccgttccct gaacatcagg gccgaggagg acgtgccgcc cgtaaagaca
      481 gacccgaacc agctacacag ggctatcgcc atcctcgtgg acaacgccct caagtactcg
      541 gatgaggagt tgcccgtcga cctggggctc tcactccagg atgggcgggt cgttatcagc
      601 gtaacggacc gcggttgcgg tatcccggaa tccgagatac cccacatctt cgaccgtttc
      661 taccgggccc agggctcgtc gcgcgccgat ggtacaggcc tgggactcgc cctcgcccat
      721 gagatcaccg accacctcgg tggtgatatc caggttcaca gccgccccaa cgccggaagc
      781 atcttctccg tactcctccc cctggccgca gacccccatg gcaaaaacgg gagccggtct
      841 ccttagtctc tacctgagat ttagcttacg ctttagcgga tttgagggta gaatatatgt
      901 tgggcagcat ggcctccgta gaatctcccc cctccctacg gaccgtgctg ctcccttatg
      961 agcaagtcag cacgccgcct ggctttgcca ggcggcgtgc tgacacgctt tcagaagacc
     1021 acgggaagac cgagaagggt aaggaggggt cgtacggggg cgagcaggat gcttaggatg
     1081 gggaggcggg tcacgaacga gaccaggatg agacccagca ggatcatggg tccgtactgg
     1141 ttcatgaagt ctacaaagcc gtcaagggcg cgcggcagga aagggaagat caccttcgag
     1201 ccgtcgagcg gggggatcgg gatcaggttg aatacgaaga gcagcgcgtt ggtgaaggca
     1261 accatgacca cgagtatgaa caggtagccg cccggttctc tgaagggtgg aagcaagtaa
     1321 agggcggcgc agacggccac gatcaggagg ttcgagatgg gtcctgctag cgcgacggcc
     1381 acggctccga acacaccacc cctgagcttg gagggtacga ccggcgtagg cctgccccac
     1441 ccgaagccgg ccaagagaag catcatgctg cctagcaggt cgagatgtga gatggggttc
     1501 agggtcaccc ttccgtcgcg ataggccgtg tcgtcgccaa ggaggtaggc cgaggtcgcg
     1561 tgggacgcct cgtgcaccgt tatacctatg accaggccga ggaggaaggc cacagcggcg
     1621 gatgggtcag cttcgaagaa tctcaggaac atggatgctc atatgataca cgcatgtcag
     1681 ctagtcagca tttcagcacg ccgcttcgcg acttgtcagc ccttcagctt gttgtcgctc
     1741 gtagccgaat gcgtgattcg cggcgtctgg atacctctgc gaatcgctat aaagcagtgc
     1801 aaatggtctg aatcttcgtt agatgtgcgg tcggttggcc agcaagaaaa aagagctgac
     1861 tgctgacaag cggaagccga aggctggagc gtgctgacta gctgatggcg cgagctgtgg
     1921 aaagcagctc gccttagccg gacttggaga cggtgacggt gacgtggttg ccggcgtcgg
     1981 aggtgtaaga ggagagttgg gggtccagga cctctgccga gtcggccacg gtgcctagta
     2041 cggtcttacc ggccgtgacg gagtcgttga cgcctgcttc gccgtcgcta agcaccaggg
     2101 ccacgctaag gcggacgtcc ggattagcgt ctggttttat ctctatgacg ttagcgttct
     2161 gaaccatggg gtcgggacgg atcgccgtaa ccgtacctgc cacaggggcg tagacggtgg
     2221 tccccgtcgc tgcgccgacg tcgacggcac cggtctgggg cccaccccgc cccgcggcgt
     2281 ccatgacgta gtagtgaatc ttctccggtg tctctcctcc ggagaggagc ccgaggacca
     2341 cgttggccga gagattcttg ccccgcggct ccattgcggc gaggctctcg ccctcgggat
     2401 gatagccgag cccggtaagg ctcgcaggac ggatcggggt ggagatactg acccctgagg
     2461 cttctgccaa caccgtggcg ggaccagcgt tgttcgggtc gatagggacg gcgcgctctg
     2521 ccatgtccgc agccttggtc tggacgaaaa cggcgaagac cagcgcgacg gcgccgagca
     2581 ccagcaggga aacggccata aagcgccttc gcctgtagac ttctccccgc ttcgcctttc
     2641 tggcctcgcg cagctcgata aagttgctct cggcgcgatc gtccgctggg cctggtcgag
     2701 tagtacttct cgtggtccgc cgatggccag gcactttccg ccgatcccca agatgttggg
     2761 ccctctgtgc gttcccccct ctcatactgg gcggggatta tacagagggg cgtgcggagt
     2821 gtaaactcgc gcgtcgcctc gtccggggct ggtatatttc gtatcgtaga gatccgatcg
     2881 gagaccgggt gtcagccaga aaactggtcg tttcgacata cctgcagtac atcgtgctcg
     2941 cggtgggaat cctactgcta gtgtcctttg tgcgccaggt cagcggcgtg ctgctgacct
     3001 tcttggccgc cggtgtcctg gcctacacct tgaatcccgt cgtccggcgc ctcgaagggt
     3061 tgcgagtgcc aagggctctg gctgttatcg gggtgttcct cgtcttgatc ttcgtcgtca
     3121 ttgtgacatt gcttctgatc atcgttccat cggtcgggca ggtacagatc gtggtccgta
     3181 acccgcaaga tctcgtcgac caggtcatcg ctctgctcaa tcggatccag aacgtgccct
     3241 acgtcggccg gtacgtggcc gagttggatc aggatcggat cctgcaactc ctgaagacaa
     3301 acgcgccgtc ggcggaacag gtcgccaacg ttgcgacagg cgtcatcggt ggggtgttcg
     3361 gagtcttcgg gaccctgttc aatttgctgc tgatggtgct tgtctccgtg tacctgctgt
     3421 tggagcggga gaagatcacc cgcgctctgc tgaatacgat tcccgagacg gtccgtgacc
     3481 agagcctgga gctattctac gcggtcgagc atacgctcgt caggtacctg agggggcaga
     3541 tcttcctgtg cgccatcatg ggtcttattg gagcggggat aatgtacttc accgtcggga
     3601 agtacgccct cccagtcggg gcctgggttg cggcgacgga gatcattccc gtcatagggg
     3661 ccttcctcgg ggccgtgccc gcggtcggga tcgcgctctt cgttccccaa ggggggtttg
     3721 tccaggctct gatcgtagcc gggctcttcc tggtagccca gcagttggaa ggcaacgtgc
     3781 tggtgccgag gatacagggc ggctcggtgg gggtccatcc gctgtgggtg ctcttcgcca
     3841 cgctggccgg aacggcactt tacgggttga tcggcgccgt cttcgccgtc ccagtagtcg
     3901 ccatcatcgc agcgacgctc cgctacctgc gcgggaccct gatcttcgaa cgctggggga
     3961 gaccgcctgt caagcctgcc gaggagccgg aaacgggcac gccggctgcc aagctgggcg
     4021 ccggccccca cggggaccgc aagggggacg acgaatgact atccctcacc gcgaacccca
     4081 gaaaccctcc ttcagagagg cttcgaagga cgcgctcctt ctcgtcctgc atccgctcgt
     4141 ccggtggctc tctatcatgc ggattcgccc cgacactctc acggttatcg gttggaccct
     4201 ggcgctcttt tccgcggtgc ttttcggcct gggatacgcg aggatcgccg gtgcggtgat
     4261 gctccttggg gggctcttcg acgcgctcga tggggccgtc gcccgcgagt cgaatacgat
     4321 gagctccttc ggcgcctttc tagactcgac cctggaccgg ctctcggagt cggcggtctt
     4381 cgtcggcctg atcttcttct acgccagctc ggcccgcccc tacgaggctt tgctagccgc
     4441 tatggcgatg acattctcac tccttacctc ttacgcccgc gcccgcgccg aaggcctcaa
     4501 catagactgc agggtcggac tgctggagcg cgcagggagg gtcgtgatcc tctcggtgtt
     4561 ctcgatcgtc ggcctgccga cggtcggcct cggcctcctg gcggcgggcg ccctcgtaac
     4621 cacagcccag cgcatcctcc acgtccgccg tgcgacccgc ggataaggag accagccgaa
     4681 cccgcgacgc ggtcggcgac tacacccgta agacggagta cgaggtctcc cgcctctcca
     4741 accgcatgaa agagggagga catccagccc cgcttggaga tcccacctca ggggtcgtgc
     4801 tcgtactcga gcagcccttc ggcccccgca ccctcgaagc cctcgagctc agcctgcagg
     4861 ccctcagact gcaacaagca tacgtgacct acgagtcgac cgggctcctc gcacaggaga
     4921 ttcaaaccac agaacccacc gtcctcgtgg caataggcgc cggcgccgcc cgcaatatcg
     4981 acgccatcga ctacccactc gtcaggcaac ccttctcaga agcggaacct ggtgtgtggt
     5041 tctcctggac gaagggcacg aaaggcctcc tcactcccgc cctcgcgccg gcgctggacg
     5101 acgacgcagc caagcgacgc ttctggtcgg ccttcctgac cctgagagac cttgcaaaca
     5161 acccataatt cccctccaaa cccacgagag ccacccgaaa cgtgttctat agacaagcct
     5221 tttcagtaac gttacaaatg ttaagttctg ttaattatct ctttcgtatg ggaaacacag
     5281 attccccgta cttaagccac tttcataagc ggggaagacc gttgtagaca gcggggtgta
     5341 ttgcacaaca tatagtgttc ttctaaagtg cccgcccaag gaggggggat tctctctagc
     5401 aggtcccctt gcaaaattta cacaacctac tggaaggagc ctgggcgtat gcctcggagg
     5461 gcgagtttgt tcgtgtttct aggtcttgcc attgcgctcg tgttcgtaac gggcatctcc
     5521 gctggggccg agccaggcgg aagcatccgg tcgaagaagg ctgatgtcgc cgcagtccag
     5581 gaccgtttgg tggagattcg cctgaaggct agctctgccg aggcggccta caacaatgcg
     5641 ctctacgaga tgaaccagtt gaacggccag atcaccagga cgacgacaga cctcagggcg
     5701 gccaaagagc gactcgccgt ggcaaaagaa actctggaag atcgggccgc gcaagtttac
     5761 aagagtggga acgtagcctt catggacgtt ctggtcgggg tggacaactt ctccgagttc
     5821 gcctccaggc tgaatctgtg gatgaggctg ctcgccaggg agagggccga gttcctggag
     5881 gtccgcgagg cccgaaacga gttggcggcg cgcagggaca ggctcgaggc ccagcgtgaa
     5941 cagagggtcg ctgctgtcga gacggctatc acccaaagag ggcgcgccaa cgaggccgag
     6001 gctcaggccg aggcttatct gaactccctc aacgccgagc tcagggatgc gatccaggca
     6061 gcgcaggatc ggcaggccac gcaggctcgc gccgccgcgg cggcgatggt cgagaatacg
     6121 ccggagcccg tggaagagcc cgagccgata ccagaggttc aggtctccca ggtcaagcag
     6181 gccagggtgc cgcgggccga tctgcaatct gaacgggccg ccgccaggcg cgaggacgct
     6241 gcacaggccg ccgccgagag ggcggagcga ctcgcagaaa agcgcgccgc cgagcgcgag
     6301 gccgccagga aagcagcgga gcaggccgca gaacaggctg ctgccgaacg ccaggctgcc
     6361 aggcgggcgg ccgagcagcg tgctgccaga cgggaagccc acagacaagc cgagctcgcc
     6421 gcccgacgtg ccgcaaagag gcaggccgcg agggaggagg cagagcatca ggccgagctg
     6481 gcagcccgtc gagccgagat agaggaagcc gccgcagaac aggccgccgc cgagcgcaag
     6541 gccgccaggc tggcagccaa gcggcgtgcc gaacggcggg ccgaagaacg cgccgcagcc
     6601 agtgcttccg ccagtgctgc cgccccagag aaggctgccg ccgagcggcg tgccgacaga
     6661 cgtgacgagc ggcgggccga agaacgcgcc gaggccagcg catctgcaag tgcctctgcc
     6721 agcgcctctg ctcccagttc gaataactct aggcgaggcg gaggtggtga tcggggaggc
     6781 agcgcgggag cttcctccgc tagcgcgact gcgagcgcct ccgcttccgg atcgcgtggc
     6841 ggcggtgggg tgagtgcttc gggcgacgag gtgctcgccg tcgccgacga tcacctggga
     6901 accccatacg tgttctctcc ccctgggccc tgtgttgctt tcgaggccga ggactgctcc
     6961 tgcttcacga tgctggtttt ccaggagttc ggcatcgccc tacctgattc accgggtggg
     7021 cagatgggct acgggacccc ggtccacggt gccccccagg cgggagacct gctcttctgg
     7081 agcgaggacg gcagtggata tatcacccac gtcggcatag ccatgggcga cgggactacc
     7141 atccacgccc aagtccataa cgaggtgacc atcaccagca taaactacgt agagggctac
     7201 gtcggggcgc ggagactgct ctagagagtg cctctggctg gcgggaagcg agagggagtc
     7261 ccagaacggg gcctcctcct acggccagga agtaagggcg gcacgtcgcg agcaggagaa
     7321 agaagccgag cgcctctacg aggaggctgc aggagaagtt tcaccgcagg cgtcgccgga
     7381 tgaggtgcgg ccagaaagag aacctaccta gagcgatttg cagcggggtc gatctgctcc
     7441 gaatcacgca ttcggctgtt agcgatccgc taagggcgta aacaacgagc caatctggcg
     7501 ccgcccataa ttccaacccc
//
LOCUS       DATMJA010000158         4730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_612581, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000158 DATMJA010000000
VERSION     DATMJA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612581"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..534
                     /locus_tag="VNT12_03750"
     CDS             328..534
                     /locus_tag="VNT12_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45263.1"
                     /translation="MQAYEARRGKVVQVRHGHWKDEFAGMRGTIQERWGNSEHSAVEV
                     LLEDGSSELFWLADLSEVDERITV"
     gene            547..732
                     /locus_tag="VNT12_03755"
     CDS             547..732
                     /locus_tag="VNT12_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45264.1"
                     /translation="MLVEHENIDGQDGIWLPFGYYLERDADLLFLRRSDNSLVAAFSA
                     EGVDLFEVELAVWADAD"
     gene            949..1833
                     /locus_tag="VNT12_03760"
     CDS             949..1833
                     /locus_tag="VNT12_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI45265.1"
                     /translation="MFDLEDRAAVATEVSLVLTATFWGLNFAATKYAAASISPLVLVA
                     LRFTVGGLLLLLVLRLLEPKSRLRRSDVLPMAALGCFGVATAQTGFTFGVSLSSAGST
                     GLIFATAPVWGLLLGATLGLERSTWKGVLGVGLSIVGVALVVLDGLTSGNASLVGDLL
                     VLIAAFCVGAYAVLSMPLLERHTPLAVATYPVLFGIPFLLLISSPQLLSLEWGSVGAG
                     PWAAVAYSAVFATAFAFAAWQRGISRIGANRVLVYQYLITVVGVTSGIVFFGETLGIN
                     KISGGVVILLGVYLARRQ"
     gene            complement(1849..2073)
                     /locus_tag="VNT12_03765"
     CDS             complement(1849..2073)
                     /locus_tag="VNT12_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45266.1"
                     /translation="MPGEIDASHFENEPPERYVRELGDVGSGVSSMFGRARLKDVAPG
                     RGSEPGQERTISVTSEKRELKPENIKIRWR"
     gene            2290..3360
                     /locus_tag="VNT12_03770"
     CDS             2290..3360
                     /locus_tag="VNT12_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HWI45267.1"
                     /translation="MDAYPIKLSYHVRDYYFGERLIPELLGKGDAPEGVVAETWEISD
                     YQETTGTVDNGPYAGRTLHDLVEEFPDELVGEGWRGPHFPLLIKFLDASNMLPVHLHA
                     DDATAREKHGEPHGKTEAWHILWAAADATILAGVEKDLSRAELVAAFKAQHYGAVMPR
                     RGIRPGDTVYVPGGILHTFGPDTLILEVQQTSDLGQFVMPFDLHGNRLDEEEWNANIE
                     ATLDELKTDYLPRPNPGLVLEDGANRRVMCCAGPHFALERWTLSEAYTEPSHLRRFLT
                     LSNVGAGMVELGYEGGSETLGRAESCILPAAIGEVRVGSRTGEATLVACYGPDLDRDV
                     VEPLREAGYSDEEIRALGEVEI"
     gene            3398..4351
                     /locus_tag="VNT12_03775"
     CDS             3398..4351
                     /locus_tag="VNT12_03775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HWI45268.1"
                     /translation="MGKIVAFGEVVSDIYRGEEISAVELGFVARPGGAPANVAVAACR
                     LGSEAAFVGSVGEDLFGDFILRALDSEGVDTSAVGRQSPPTRTSLAFVEIGADGDREF
                     TFYRSSPAADELLSPEDVAEDMVSGASFVNFGSIPLIREPVRSATHRIAEVARELDVP
                     VAFDVNFREHLWESAEAAREAVDPLLDLSTIVKLGDNELSPLLGTNDIEEAAGMLLDR
                     GVSLVLVSKGPEGAFYAAREFSGEVPTFDVGEIVDATGAGDAFLAAALVHLSDKPLEE
                     DRVHEATLMGCAAGALACTDYGAMRALPTKEELEQFMKADT"
     gene            complement(4405..>4730)
                     /locus_tag="VNT12_03780"
     CDS             complement(4405..>4730)
                     /locus_tag="VNT12_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HWI45269.1"
                     /translation="TAEELREHVSASLGFPSRLVADAGRRIEAIAVLGGSGSSFIPEV
                     AASGARAYVTGDVDYHDALLAESLGLTVIDAGHAATELPSLEPLALRLAELVDVPVEA
                     SQVRG"
BASE COUNT          838 a         1421 c         1522 g          949 t
ORIGIN      
        1 cactcggtga ggcatagagg gcctcagaca gaaaactaat acagctatac gcatgctttt
       61 gggatgactt tccctccacg aggaaggccc gggtcgaggc aacacgacga attgatcgtt
      121 cacttagctt acttgtgtct caggcgatga ctctatgctt cccatggcgg gtcttgcgga
      181 acgctttgta tccttcaata cacaaaaggg tgttcaagcc tgttatatat cctgatcggg
      241 aaagggaaag acgtttgggg tcgggtcctg cattccgctg gtgaacggct ctggctccaa
      301 cgggggatgc acaggaggtt actgctcttg caggcttacg aagctcgtag aggcaaagtg
      361 gtgcaggtga ggcacggtca ctggaaagac gagtttgccg gaatgcgcgg caccatacag
      421 gagcgctggg gcaattcaga acatagtgct gtcgaagttc tgctagagga cgggagttct
      481 gagttgttct ggctcgctga cctgtctgag gtggatgagc gcatcaccgt ctagtagttg
      541 ggtctattgc tagtggagca tgagaacata gacgggcaag atgggatctg gttgccgttc
      601 ggctattacc tggaacgtga cgcagacctg ctgttcctac gtaggtccga caattctctc
      661 gtggcggcgt tcagtgccga gggcgtggac ctcttcgaag tggagttggc ggtttgggca
      721 gatgccgatt agcgctctgg gcatttccct cccgatatgc ccaatctggt gcccttacct
      781 gttctggcca aaatagtaca aagcgtttag cctgctggca tcttccttgg cttgaagtgg
      841 gctcgatagt ctgccggcgt ggtgaagcct gttccgagcc tctgtgtgga tagataagat
      901 ccggaggcag cctacccaat cattgcaccc acggtacaat ccgcacctgt gtttgatctc
      961 gaggacaggg ctgctgtggc gacggaggtc tcgctggttc tgactgcgac cttctggggc
     1021 ctgaacttcg ccgcgaccaa gtatgccgcc gcgagtatct cgccattggt gctagtcgcc
     1081 ctgcggttta cggtcggtgg gctgttgtta ctgctcgttc tgcggcttct cgaaccgaag
     1141 agcaggctcc gaaggagtga tgtgctaccg atggcggcac tgggttgctt cggggtggcc
     1201 acggcccaga cgggtttcac gttcggcgtt agcctctcca gcgccggcag cacaggcctt
     1261 atattcgcga cggcgccggt ctggggatta ctgcttggcg cgaccctcgg tctcgagcgg
     1321 tcgacgtgga agggtgtgct cggcgtcggg ctctccatcg tgggcgttgc tctggtcgtc
     1381 ctggacgggc tgacgtcggg gaatgccagc cttgtggggg acttgctggt tctaatcgcc
     1441 gccttctgcg tcggcgcata cgcggttctc tcgatgcctc ttctggagcg ccacacgccg
     1501 ctcgcggtgg cgacctatcc tgtgttgttc gggatcccct tccttctgct catctcctcc
     1561 ccacagctac tgagtctgga gtggggaagc gtgggagcag gaccctgggc cgcagtcgcc
     1621 tactccgccg tcttcgccac cgcgttcgcg ttcgcggctt ggcagagggg gataagccgc
     1681 atcggggcga atagggtgct cgtctaccag tacctcatca ccgtcgtcgg tgtcacgtct
     1741 ggcatcgtgt tcttcggcga gaccctgggg atcaataaaa tctccggcgg agtcgtgatc
     1801 ctgctcggcg tctatctggc tcgcaggcag tagagaaggt ttcaggtatc agcgccatct
     1861 gattttgatg ttctccggct tgagttctct cttttcgctg gtgacggaga tcgtacgctc
     1921 ttgtcccggc tctgaccccc tcccaggagc aacgtctttg agccgtgcgc gaccgaacat
     1981 agagctcaca ccgctcccta cgtccccgag ctcgcggacg tagcgttcgg gcggctcgtt
     2041 ctcgaagtgg ctggcgtcga tctcgcccgg cacgatagcc tgtggcgcct gtcaggagga
     2101 cacgccggcg ctgctctgca tgcgcatcgt tcgtttcgta gagagccaag agtcctctcc
     2161 caggtcgggg atccgtcccc cttatctact cacaatcgct ggcctcgtgc atcgcgagcc
     2221 gctgtcaggg agcgtcccga ccggtgtaga atacccctgc ttagaagcga ggggagagga
     2281 agatgggacg tggacgccta cccgatcaag ttgagttacc acgtgcgcga ctattacttc
     2341 ggggagcgtt tgatccccga gctgctgggc aagggggacg cgccagaagg cgttgtggct
     2401 gagacctggg agataagcga ctaccaggag acgacgggga ccgttgacaa tggtccctat
     2461 gcaggacgca cgctccacga tctcgtcgag gagttccctg atgagttggt gggggagggc
     2521 tggcgcgggc cgcatttccc gctcctgatc aaattcctcg acgcctccaa catgctgcca
     2581 gtccacctgc acgctgacga cgcgaccgcg agggaaaaac acggcgagcc ccacggcaag
     2641 acggaggcgt ggcatatcct gtgggccgcc gccgacgcga cgatacttgc cggggtcgag
     2701 aaggacctgt cccgagcgga actcgtcgcg gctttcaagg cccagcacta cggcgccgtc
     2761 atgccgcgcc gcggaatccg gcctggcgac actgtttacg ttcccggcgg catcctgcac
     2821 accttcgggc cagacaccct gatcctggag gtccagcaga cctcggacct gggccagttc
     2881 gtaatgcctt tcgacctaca cggcaaccgc ttggacgaag aggaatggaa cgcgaacata
     2941 gaagccactc tcgacgagct gaagacggac tacctgcccc gtccgaaccc cggactcgtg
     3001 ctcgaagacg gcgccaacag gcgtgtcatg tgctgcgccg gtccgcactt cgccctcgaa
     3061 cgctggacgc tctcagaggc ttatactgaa ccttcgcacc tgcggcgctt cctgacgctg
     3121 tcgaacgtcg gcgcggggat ggtggagcta gggtacgagg gcggttccga gacgctcgga
     3181 cgcgccgaga gctgcatctt gcccgccgcg ataggagagg tgagggtggg ctccaggaca
     3241 ggggaggcca ccctggtggc gtgctacggc cccgacctcg atcgcgatgt tgtagaaccc
     3301 ctgcgtgagg ccgggtactc cgacgaggag atacgggctc tgggcgaggt agagatctag
     3361 ccggtggaga accagataac agggtcggga ggaagacttg gggaagatag tcgcttttgg
     3421 cgaggtcgtc tcggacatct acaggggaga ggagatctcg gcggtcgagc ttgggttcgt
     3481 cgccaggccc gggggcgcgc cggcgaacgt cgcggtcgcc gcctgccgcc tcggctccga
     3541 ggccgccttc gtgggcagtg ttggcgagga tctcttcgga gactttatcc tgcgggccct
     3601 cgactccgaa ggcgtggata cttccgctgt cggccgccag agtccgccga cgcgaacctc
     3661 gctggccttt gtggagatag gcgctgacgg agaccgcgag ttcaccttct accggtccag
     3721 ccccgccgcc gacgagctgc tctcgcccga ggacgttgcg gaggatatgg tctcaggagc
     3781 gtcttttgtg aacttcggca gcatccccct catccgcgaa cccgtccgtt cggctacgca
     3841 ccgcatcgcg gaggttgcac gcgagctcga cgtccccgtg gccttcgacg tcaacttccg
     3901 cgagcatctg tgggagagcg ccgaggccgc aagagaagcc gtggacccgc ttctggacct
     3961 ctccacaatc gtcaagctgg gcgacaatga gctctcgcca ctgctcggaa ccaatgacat
     4021 cgaggaggcc gccgggatgc tgctggatcg cggcgtctcc ctggtgctcg taagcaaggg
     4081 accagaaggc gcgttctacg ccgccaggga attcagcggc gaagtgccca ccttcgacgt
     4141 gggcgagatc gtggacgcca ccggcgcggg agacgctttc cttgccgccg ccctcgtcca
     4201 cctctcagac aaacccctgg aggaagacag ggtgcacgag gcgaccctga tgggctgcgc
     4261 cgccggcgcc ctcgcctgca ccgactacgg cgccatgcgc gccctgccga ccaaggaaga
     4321 actcgaacag ttcatgaaag ccgatacctg aagctgcgga ttgcctgtag gggcgattcg
     4381 cgaatcgccc acacacgaat cgctctagcc tctcacttgg ctggcctcca caggcacgtc
     4441 caccagttcc gcgagtcgca gggcgagcgg ctcgagcgaa ggcagctccg tggcggcgtg
     4501 tccggcgtct atcacggtca ggccaagcga ctctgcaagc aacgcgtcgt ggtagtcgac
     4561 gtcccccgtt acgtaagcgc gtgcgcccga ggcggctacc tcggggataa aggaactgcc
     4621 tgatcccccg aggacagcga tggcctctat ccttcgtccc gcatctgcga cgaggcgtga
     4681 agggaagccc aacgacgccg agacgtgttc gcgtaactcc tcggctgtga
//
LOCUS       DATMJA010000159         5338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_257195, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000159 DATMJA010000000
VERSION     DATMJA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257195"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..2803
                     /locus_tag="VNT12_03785"
     CDS             356..2803
                     /locus_tag="VNT12_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI45270.1"
                     /translation="MEDRARVVIVGAGIVGCGAAEHLTRLGWREVVLLDQGPLFEAGG
                     STSHAPGLVFQTNPSKTMTNLASYGVERYSELELDGKRCFYPVGGLEVATTPERWKDL
                     KRKHGIATSWGVQSELLAPEECVEKSPLLDPKRIWGGFYVPSDGIAKGVRISEAMARE
                     ARTRGAKFYGETEVTGIEVENGRVKAVETSRGRIETEYVLSCAGMWGPRIGRMAGAFV
                     PLLTPMQHQYAWTTPVLGLEPVSEESDHVILRHQDHAMYFRQVGERYGIGSYKHRSMP
                     VSPDEIPRKHEPGWEMPSIMEFTPEDFEGPWEESRRLLPALQKTKIERGINGLFSFTP
                     DGGSLLGESREVRGFWMAEAVWVTHAAGAARMIAEWMTDGAPSIDPSGLDVHRFDDFQ
                     KSPSYVLTRSSQSFQEVYDIIHPQQPMEEPRPLRTSPFYPRQRELGAYFLEASGWERP
                     QWYGANDSMLAEYDVPEMEEWAGRYWSPIVGAEHQAARERGALIDMASLKKAEVSGPG
                     ALGFLQKLNTNQLGKPIGSVTYTLMLDQKAGIRSDITVARLGEEHFQLGLNGPRDIEW
                     MERHLPDDGSVQIRDISGSTCCVGVWGPQARELVQSLSPDDLSNEAFGFFKARHIYVG
                     EVPVVALRVSYVGELGWELYASADLGLRLWDVLYRAGRPLGMIAAGRGAFSGMRLEKG
                     YRLWGTDMTTEHDPYEAGLGFAVKLDKGEFVGREALLRRKEEGPRRRLSCLLLDDPSV
                     VVMGSEPVYAEGRPVGYVTSAAYGYSIGQSIAYAWLPPGLSEEGAKVEIEYFGKRHGA
                     TVAEEPLFDPAMKRMRG"
     gene            2819..3787
                     /locus_tag="VNT12_03790"
     CDS             2819..3787
                     /locus_tag="VNT12_03790"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWI45271.1"
                     /translation="MSTKYASGHRVAVSSVLDGIGDTPLVPVEGIYCKLEYLNPSGSI
                     KARIAKYMVERAEREGFLKPGDTIVEASSGNTGNALSMVAAVKGYRMLVVMPEGMSSE
                     RAVISRAFGAEVLEVGHFHVNEALATAKKLGRQDGYFCPSQFESEWNVEENRVWLGPE
                     ILSQLPEGAVPDALVMGVGTGGTLVGVGQAFRAVNPDVKIFAVEPDESCTILCGETGK
                     HAIEGISDGFVPGIFERHGGLVDGVLSVMSKESIEAMRCLAREHGMFVGPSSGAHLVV
                     ARKVREEHPELKTVVTLFCDEGEKYISDHFRKPGGGEMQRSSCGPS"
     gene            3852..5051
                     /locus_tag="VNT12_03795"
     CDS             3852..5051
                     /locus_tag="VNT12_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWI45272.1"
                     /translation="MAVNDNPGILLYTRLFRSPYFYGSRQHGVQKYSVYNHHYHPRYY
                     RDPVEEYWQLLNGVTMWDVGGAERQVEITGPDAFEFTNMLTPRDLNKCNVEQCKYAFI
                     TNAQGGIINDPVLLRLEENHFWLSLADSDVLLWAQGLAYNAGMNVEIREPDVGPMQIQ
                     GPKSKEVMSDLFGESILEVPYYYMVYRELDGMELVISRTGYSGELGYELYLKNASRDG
                     LKLWDRVLQAGEPYGISPIGPCHIRRIEGGILALGCDMWYDTNPYEVGYGYKWMVELT
                     QEQDFIGKEALKKISEEGVSRKLVGVELGGSNLGSYNDGSMIDYFPVYKNGERIGKVT
                     SACHSPRLDKNIGFAMVPVEYSEIGIELEIERPEETVPAVVADRVFFKPEHAEQDLSA
                     MSSNKGA"
     gene            5157..>5338
                     /locus_tag="VNT12_03800"
     CDS             5157..>5338
                     /locus_tag="VNT12_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45273.1"
                     /translation="MNKTDKEHEVRDALAEALRRPRYEVIPLGGVEKAIVEHVPNSIK
                     LTVTASPKKGIEETLRL"
BASE COUNT         1073 a         1538 c         1845 g          882 t
ORIGIN      
        1 gggccgcggc acgacgcccc cgatttaaag ctgaaagctc ccaggctgtg gtcgagcaga
       61 cgaggagaac gaaaaaccta gcggggcctc tgatggtcgt tggagctcgg acggtggtct
      121 ttatcgagaa taccgtgcgc cgctcaaaaa gtgggcgcgg gtgcttgact cggcggcggg
      181 cgtatgttag ttgtttcact tatgggaaaa cggtgcgcat tgcgcaacaa caatcggcct
      241 gctggcgtat gaggtccgta ggggatcttc gccggaggtg gagcgagcct cttgttcgtc
      301 ctgagttgct cgggcgttgt gtccttgtct ccacgagacg tacggaggaa tagcggtgga
      361 agatagggct agggttgtga tcgtcggagc cggcatcgtt ggctgcggcg ccgccgagca
      421 cctgacgagg ctcggctggc gggaggtggt gctcttggat cagggaccac ttttcgaggc
      481 gggcggctct acctcgcatg ccccgggact cgtattccag accaacccgt cgaagacgat
      541 gaccaacctc gcgagctacg gcgtggagcg atactcagag ctcgagctag acgggaagcg
      601 gtgcttctac ccggtgggcg gcctcgaggt cgcgacgacc cccgagcggt ggaaggatct
      661 gaagaggaaa cacgggatcg cgacctcatg gggcgtccag tcggagctcc tcgcgcccga
      721 ggagtgcgta gagaagagcc cgctcttgga cccgaagagg atatggggcg gcttctacgt
      781 gccctcggac ggcatcgcca agggtgtcag gatcagcgaa gcgatggcgc gggaggcccg
      841 aacgcgcggt gcgaagttct acggcgagac cgaggtgacg gggatagagg tcgagaacgg
      901 acgcgtcaag gcggtcgaga cctcgcgggg ccgcatcgag acagagtatg tgttgagctg
      961 cgccgggatg tgggggccgc gcatcgggag gatggcgggg gccttcgtgc cgctcctgac
     1021 gccgatgcag caccagtacg cctggaccac gccggtactg gggcttgaac cggtctccga
     1081 ggagtcagac catgtcatcc ttaggcacca ggaccacgcg atgtacttcc ggcaggtcgg
     1141 cgagcgttac gggatcggct cctacaagca ccgctcgatg ccagtctcgc cggacgagat
     1201 cccgaggaag catgagcccg ggtgggagat gccctcaatc atggagttca ccccggagga
     1261 cttcgagggg ccatgggaag agtcgcgcag gctgcttcct gcgctgcaga agaccaagat
     1321 agagcgaggc ataaacgggt tgttctcttt cacgccggat ggtgggtcgc tgctcggcga
     1381 gtccagggag gtacgcggct tctggatggc ggaggccgta tgggtgacgc acgcggcggg
     1441 cgcggccagg atgatagccg agtggatgac ggacggcgcc ccgagcattg acccgagcgg
     1501 gctggacgtt caccgcttcg acgacttcca gaagagcccc tcctacgtgc tgacccgcag
     1561 ctcgcagtcc ttccaggaag tctacgacat catccacccc cagcaaccta tggaggagcc
     1621 gcgaccgttg cggacaagcc ccttttaccc acgtcagagg gagctcgggg cgtacttcct
     1681 ggaagcctcc ggctgggagc gcccgcagtg gtacggggct aacgactcga tgctcgccga
     1741 atacgacgtc cccgagatgg aggagtgggc ggggcggtac tggtcgccta tcgtcggggc
     1801 tgagcaccag gcggctcgcg agagaggcgc tctcatcgat atggcttcac taaagaaggc
     1861 cgaggtcagc ggccctgggg cgctcggctt cctgcagaag ctcaacacga accagctcgg
     1921 caaacccata ggcagcgtca cctacactct gatgttggac caaaaggcgg gtatcaggag
     1981 cgacattaca gtcgcgcggc tcggtgagga gcacttccag ctcgggctca acggaccgcg
     2041 ggacatcgag tggatggagc gtcacctgcc cgatgacggc tcggtgcaga ttcgggacat
     2101 ctccggcagc acctgctgcg ttggggtctg ggggccgcag gcccgcgagc ttgtgcagag
     2161 cctgagtccc gacgacctct ccaacgaggc gttcgggttc tttaaggcga ggcacatcta
     2221 cgtcggcgag gtgcccgtcg tggcgctcag ggtctcctac gtcggcgagc tcggctggga
     2281 gctctatgcc tccgctgacc tgggcttgcg gctctgggac gtgctctacc gggcgggacg
     2341 gccgctcggc atgatcgccg caggacgcgg ggccttcagc ggcatgaggc tggagaaagg
     2401 ttaccggctg tgggggacag acatgaccac cgaacatgac ccttacgaag cagggctcgg
     2461 tttcgcggtg aagctcgaca agggagagtt tgtcggcaga gaggcgctgc tgaggcgcaa
     2521 agaggaaggc ccgcggcgca gactttcgtg tctcctcctc gacgacccga gcgtggtcgt
     2581 catgggcagc gagccggtct acgccgaggg cagaccggtc ggctacgtca ccagcgcggc
     2641 ttacgggtac tcgatcggac agagcatagc ctacgcttgg ctaccgcccg gactctcgga
     2701 ggagggcgcg aaggtggaga tcgaatactt cggtaagcgc catggcgcga ccgtcgcgga
     2761 ggagccgctc tttgacccgg ccatgaagcg tatgcggggc tgaggaggca gggccgtgat
     2821 gagcaccaag tatgcaagcg ggcatcgggt tgcggtctcc agcgtgctcg atggtattgg
     2881 cgatacaccg ctggtgccgg tcgagggtat ctactgcaag ctcgagtacc tgaacccctc
     2941 gggctccata aaggcgcgca tcgccaagta catggtcgag agggccgaga gagagggctt
     3001 cctaaaaccc ggcgacacga tagtcgaggc ttcgagcggc aacaccggca acgccctctc
     3061 gatggtggcc gccgtaaagg gctacaggat gctcgtggtc atgcccgagg ggatgtccag
     3121 cgaacgcgcc gtcataagcc gcgccttcgg cgccgaggtc ttagaagttg ggcacttcca
     3181 cgtcaacgag gcgctggcca cggcgaagaa gctcgggcgg caggacggct acttctgtcc
     3241 ctcgcagttc gagtcggagt ggaacgtcga ggagaaccgc gtgtggcttg ggcccgagat
     3301 actctcccag ctccccgaag gggccgtccc cgacgcgctc gtcatgggtg taggcacagg
     3361 aggcaccctg gtgggcgtcg gccaggcctt ccgcgcggta aacccggacg taaagatctt
     3421 cgctgtcgag ccggacgagt cgtgcaccat cttgtgcggc gaaacgggca aacacgccat
     3481 agagggcatc tccgatggat tcgtacccgg catcttcgag cggcatgggg gattggtgga
     3541 tggcgtgctt tccgtcatga gtaaggagtc aatcgaagcg atgcggtgtt tagcgcggga
     3601 gcacggcatg ttcgtcgggc cgagcagcgg cgcgcacctc gtagtggccc gcaaggtccg
     3661 cgaggagcac cccgagctga aaacggtcgt taccctcttc tgcgacgagg gcgagaagta
     3721 cataagcgac cacttccgca aaccgggggg aggagagatg caacgaagta gttgtggccc
     3781 aagttagtga agcgggcgca agcggtccac gggttaatga tcgcggagaa ccaggaaagg
     3841 agataaccgc aatggcggta aacgacaacc caggtatctt gctgtacacg agattgttca
     3901 ggtccccata cttctacggc tcacgtcagc acggagtcca gaaatatagc gtctacaacc
     3961 accactacca cccgcgctac tacagggacc cggtcgaaga gtactggcag ctcctcaacg
     4021 gcgtcacgat gtgggacgtc ggcggggccg agcggcaggt ggagatcacc ggtcccgacg
     4081 cctttgagtt cacgaacatg ctcaccccgc gcgacctcaa caagtgcaac gtcgagcagt
     4141 gcaagtacgc cttcatcacg aacgcccagg gcggcatcat aaacgacccg gtgcttctgc
     4201 gtctcgagga gaaccatttc tggctctccc tggccgacag tgatgtgctg ctctgggccc
     4261 agggcctcgc ctacaacgcg gggatgaacg tggagatcag ggagcccgac gtgggcccga
     4321 tgcagatcca gggcccaaag tccaaggagg tcatgtccga cctgttcggc gagagcatcc
     4381 tggaggtccc ctactactac atggtctaca gggagctcga cgggatggag ctggtcatct
     4441 cccgcaccgg ctattccggg gagctcggct acgagctcta cctgaaaaac gccagccgcg
     4501 acggcctcaa gctctgggac cgtgtgctcc aggccggcga gccgtacggg atctcgccga
     4561 tcgggccgtg ccacatccgg cgtatcgagg gcggcatcct ggccctgggc tgcgacatgt
     4621 ggtacgacac caacccctac gaggtcggct acggctacaa gtggatggtg gagctgacgc
     4681 aggagcagga ctttatcggc aaagaggcgc tgaagaagat cagcgaggag ggcgtcagcc
     4741 gcaagctggt cggcgtcgag ctcgggggca gcaacctggg gtcgtacaac gacggctcga
     4801 tgatcgacta cttcccggtc tacaagaacg gcgagaggat agggaaggtc acctccgcct
     4861 gccactcgcc gcggctggac aagaacatcg gctttgcgat ggtgccggtc gagtacagcg
     4921 agataggcat cgagctcgaa atcgagcgtc cggaagagac ggttccggcc gtcgtggccg
     4981 accgggtctt ctttaagccc gagcacgccg aacaggattt gagcgccatg agttccaata
     5041 agggggccta gcgccggagg aagaggttgc cacgggcgcg tgtccgcgga cgccgtgccc
     5101 gtggcaacca gcgaagaagg catccgagcc tcgaagccgg cggaaagatc gtgatcatga
     5161 acaagactga taaggaacac gaggtaaggg acgcgctggc cgaggccctg cgacgcccca
     5221 ggtacgaggt gataccgcta gggggcgtgg agaaagcgat cgtcgagcac gtacccaata
     5281 gcatcaagct tacagtgacg gcgtcaccca agaaaggcat cgaagagacg ttgcggct
//
LOCUS       DATMJA010000160         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_435074, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000160 DATMJA010000000
VERSION     DATMJA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435074"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VNT12_03805"
     CDS             complement(<1..566)
                     /locus_tag="VNT12_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWI45274.1"
                     /translation="MRAVRTSQAVPVSRGNKQEKVSIAHRLRRSDVLLFLTAVALGGI
                     GVLAIYAAEADYRQLYAVNQALGLAAGLVGAVVLALLDYRWLGRHLRFVYGVTIVMLV
                     SVLVAGFSVNGAQSWIGVGPVEMQPSEFAKPLSIVVLAGYVAENSFAHNMTFLKALGI
                     ISVPVLLVLALLDYRWLGRHLRFVYGVT"
     gene            765..1064
                     /gene="rplU"
                     /locus_tag="VNT12_03810"
     CDS             765..1064
                     /gene="rplU"
                     /locus_tag="VNT12_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWI45275.1"
                     /translation="MFAVVKSGGKQYRVKEGQELVVDRLKGEVGDSLELPVGFAVDEG
                     GFDLGEKKARVEILDHLRGDKIHVYKYKAKKDYRKKTGHRQELTRVKVLEVSDGS"
     gene            1054..1329
                     /gene="rpmA"
                     /locus_tag="VNT12_03815"
     CDS             1054..1329
                     /gene="rpmA"
                     /locus_tag="VNT12_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWI45276.1"
                     /translation="MAHKKGGGSSRNGRDSAGRRLGVKAYGGQVVTAGSIIVRQRGSK
                     VHPGQNVGKGSDDTLFARVSGRVDFDRSRGKSVVSVIEEPVLQEATA"
     gene            1361..>2499
                     /gene="obgE"
                     /locus_tag="VNT12_03820"
     CDS             1361..>2499
                     /gene="obgE"
                     /locus_tag="VNT12_03820"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWI45277.1"
                     /translation="MQFVDEASFIVRAGRGGDGSVSFNREKYKPRGGPDGGRGGDGGD
                     VILCSTEDLSTLEPHAYRNVVKAGRGGHGSSNNRAGERGKDAVLEIPVGTQVFDDDGL
                     LADLSEPGETFVVAAGGEGGRGNGSFASSTRQAPAFREKGLPGEEREIRLELKVLSDV
                     GLVGLPNAGKSSLLRALSAARPRVGDYPFTTLSPQLGVVDEKAYRGRFVVADIPGLIS
                     GASEGRGLGNRFLRHVARARLLALVLDASEDPEGAEGTLRAELHAARLSEKPTVVVLN
                     KVDLLDEELRTYLWEAFPVAPQVSATTAEGVAALRDTFERRLREMGEHAVSEVPEREH
                     RVFRPTWKGVRVEHENGGYVVSGEGVERLALKTDWGNREGVERFQ"
BASE COUNT          497 a          751 c          850 g          401 t
ORIGIN      
        1 atcgttaccc cgtagacgaa cctgaggtgt cgcccgagcc acctgtaatc cagcagcgcg
       61 agcacgagca acacgggaac agatatgatg ccaagggctt tcaggaaagt catgttgtgc
      121 gcaaaactgt tctcggccac ataaccggcg agcaccacga tcgacagtgg cttggcgaac
      181 tccgaaggct gcatctcgac gggcccgact ccgatccagc tctgcgcccc gttcaccgag
      241 aagcctgcga cgagcacgga caccagcatc acgatcgtta ccccgtagac gaacctgagg
      301 tgtcgcccga gccacctgta atccagcagc gcgagcacga cggcgccgac aagaccggca
      361 gccagcccga gggcctggtt gacggcgtag agctgcctgt agtccgcctc ggcagcatag
      421 atggcgagga ccccgatccc accaagcgcc acagcggtga ggaaaaggag cacatcggag
      481 cggcgtagcc gatgcgcgat gcttaccttc tcttgtttgt tgcccctgga gacggggacc
      541 gcttgactgg ttcttacggc acgcacaacc caaagataca gaatcttcgg gcgttcggca
      601 acgcccgcag gaagagcccg ctaccaggcc ttgaccgcca cctgctgctc ggacgccagg
      661 gccgcctttg cttgtggtcg acacggagca tggcgggtat actgttgccc cgtggggtgc
      721 gttttgtacg cccgcagtag cctgataagt tctaggagat cgagatgttt gcggttgtaa
      781 agagcggtgg aaagcagtac agggtgaaag agggtcaaga gctcgtcgtc gaccgcctaa
      841 agggcgaggt aggggattcc ctggagctac ctgtcggctt cgcagtcgat gaaggggggt
      901 tcgacctcgg cgagaagaag gccagggtcg agatcctgga ccatctgcgg ggagataaga
      961 tccacgtcta caagtacaag gcgaaaaaag actacaggaa gaagaccggg cacaggcaag
     1021 aactgacccg ggtaaaggtg ctggaggtat ctgatggctc ataagaaggg cgggggctcc
     1081 tcgcgtaacg gccgagactc ggcaggcagg aggctaggag taaaggcata cggtggccag
     1141 gtggttactg caggaagcat catcgtgcgc cagcgcggct cgaaggtgca cccgggacag
     1201 aacgtcggga agggctcaga tgatacgctc ttcgccaggg tctcaggcag ggtcgacttc
     1261 gaccggtctc ggggcaagag tgtcgttagc gtgatcgagg agcccgttct gcaggaggcc
     1321 acggcctgac catcgtccgt tacgggggtc tttagcccct ttgcagttcg tagatgaagc
     1381 cagcttcatc gtccgcgcgg gtcgcggggg cgacggcagc gtctctttca accgcgagaa
     1441 gtacaagccg cgcggcggcc ccgacggtgg tcgcggcggc gacggcgggg acgtgatcct
     1501 gtgctccact gaagacctct caaccctcga accgcacgcg taccgcaacg tcgtgaaagc
     1561 gggccgtggc gggcacggtt catccaacaa cagggcgggc gagcgcggca aggacgcggt
     1621 gctcgagatc cccgtcggaa cccaggtctt cgacgacgac ggcctcctgg ccgacctctc
     1681 ggaaccggga gagactttcg tcgtcgccgc cggcggcgag ggcggacgcg gcaatgggtc
     1741 cttcgcatcc tccacccgcc aggcgccggc attcagggag aagggtctgc caggtgagga
     1801 gcgtgagatc cgtctcgagc tcaaggtcct ctcggacgtt gggctcgtcg gcctgcccaa
     1861 cgcgggaaag tcttcgctgc tccgagcact aagcgccgcc cgtccgaggg tgggcgacta
     1921 tcctttcacc accctctcgc cccagctcgg ggtcgtggac gagaaggctt accgcggacg
     1981 gttcgtggta gcagacatcc ctggcctcat ctcgggggcc agcgagggca ggggcctcgg
     2041 caaccgcttt ctcaggcacg tggcgcgggc ccggctactg gcgctcgtgc tcgacgcgag
     2101 cgaagacccc gaaggggccg aaggcacgct gcgggccgag ctccacgccg ctaggctctc
     2161 ggagaagccc acggtcgtgg tcctgaacaa agtcgacctc ctcgatgagg agctcaggac
     2221 gtacctgtgg gaggcctttc cggttgcccc tcaggtctcc gcgaccacgg cggaaggtgt
     2281 cgcagcactg cgcgacacct tcgagcggag gctgcgcgag atgggtgagc acgccgtcag
     2341 tgaggtgcct gagcgtgagc atcgcgtctt caggccaacc tggaaggggg tcagggtcga
     2401 gcacgagaac ggtggctacg tcgtctccgg ggagggcgtc gagcggctcg ccctcaagac
     2461 agactggggc aacagggagg gcgtcgagcg ctttcaaca
//
LOCUS       DATMJA010000161         3761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_553618, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000161 DATMJA010000000
VERSION     DATMJA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553618"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VNT12_03825"
     CDS             complement(<1..392)
                     /locus_tag="VNT12_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI45278.1"
                     /translation="MNGASAERVRAFLVHWLFTSLYGMALDEYLALLRKHRFAVDAPY
                     LPRSAFMLLSCTLTSLIRAYEDRRYGPEIAGVKVREPLFVLGHWRSGTTYLHNLLALD
                     KRFAHPNVWRVLNPHTFLSTERYSDIVKL"
     gene            complement(389..1573)
                     /locus_tag="VNT12_03830"
     CDS             complement(389..1573)
                     /locus_tag="VNT12_03830"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWI45279.1"
                     /translation="MVVEVLQLMLLALVALSLLSTLLSHAFAWYYFASYDRRFPRGEY
                     APPVSVIKPVKGVDQSALANFRGFCEQDYPSDYEVVFCVEGRDDPSVLVIERIIEEYP
                     DRFVRLIFSDSEDARSVGKLKNMIAGYAHSSYDVIVFSDSDAQVPPNFLRETVTCLEV
                     PEVGLGFGAPAYVGARDWAAALMAFSANPFVLRLASMCLFRAFDGAVGTTMVTRREAI
                     ERVGGLERFGRHSTDDLPLGRALRQEGYHIHLLKQPARVSHPHYTFGRWWTQMHRWLV
                     IIRRYWPNNFLITSAMDLAPWWALLYLLVSLLAGDGIVFGACLLAAALGTPLVSQAVV
                     NARFVKEEGLWRYLWVVLVHEAVRLALVLYAAFTDEVVWRGQRMRVASDGTVELVGRE
                     LR"
     gene            complement(1591..2907)
                     /locus_tag="VNT12_03835"
     CDS             complement(1591..2907)
                     /locus_tag="VNT12_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HWI45280.1"
                     /translation="MSHRLSYLSPKEKRALLAQLLLRKSGNLDKLLEQVAVNVENLEA
                     EAALDPRIRPTGTVSTEVASRPERVLLTGATGFLGSFLLAELLRHTRAEIHCLVRAPN
                     VEEGERRIRGSLETYALWDEKRSPRIFPVAGDLSEPLLGLGDERFEELADEIDVVYHN
                     GASVNWVYPYDALKPTNVLGTQEVLRLASRHGTKPVHFVSTLGVFPLVGRSDAGVVRE
                     DDDLDHGGSLYNGYTQTKWVAEKLVEIARARGLPVSVYRPSLIAGDSRTGAWNKDDFT
                     CKMIKSWVGLGNVPDVNTELNMVPVDYVSRAIIRLSLQEGSLGKRFHLANSHPVKIDN
                     LISWIEAFGYPLKRIPYDRWRTELLNPVKGLREDAMYSAAPLLSMSATIEGPKMVRAV
                     PEFDRRNTTAALASTGISCPPVNAGTFEKYLVYLVRSGFLASPTGG"
     gene            complement(2904..>3761)
                     /locus_tag="VNT12_03840"
     CDS             complement(2904..>3761)
                     /locus_tag="VNT12_03840"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HWI45281.1"
                     /translation="GAWNLHRLTQDDELDFFMLFSSGASVLGSPGQANYVAANEFLDA
                     LAHYRRAAGLPATSINWGAWAEVGLAVRKDRARHLSNQGLVAFTPEQGARLLGWILER
                     NPVQVMAAAVDWARLLSSYSSPLLSELAREVNLSPSHKRRGDGLTKEKLQAAPPEERR
                     PLAEKFLAEQLAAVLRCPTSKVDIHQPLNRLGIDSLMAVELKNRVEADLETSVPVTAL
                     LQGPSLSQLATHLLEGLDAPTAADAASVSAPEETRERLVEAQVEGLSDEEVDALLRGL
                     VEEEQETRG"
BASE COUNT          717 a         1215 c         1166 g          663 t
ORIGIN      
        1 agtttgacga tatccgagta ccgctcggta ctgagaaagg tgtgcgggtt gaggactcgc
       61 cacacgttgg gatgcgcgaa ccgcttgtcc aaggcgagca ggttgtgcag gtacgtcgtg
      121 ccgctgcgcc agtgcccaag aacgaacaag ggctccctga ctttgacgcc tgcgatctcg
      181 ggaccatacc tcctatcttc gtaggcccgg atcaaagatg tcaaggtgca agagaggagc
      241 atgaaggccg accggggcaa gtagggtgcg tccacggcga agcgatgctt gcggagcaac
      301 gccaagtact cgtccagggc catcccgtac aggctagtga acagccagtg gaccagaaac
      361 gcgcggaccc tctctgccga ggcgccgttc atcgtagctc cctaccgacg agctcgaccg
      421 tgccgtccga ggcgacccgc atcctctgcc cccgccagac aacctcgtcc gtgaaagcgg
      481 cgtataacac aagcgccagc cgcaccgcct catgcaccaa cacgacccac aggtaccgcc
      541 agagcccctc ctccttgacg aaccgcgcgt tgaccacggc ctgcgagacc aacggcgtgc
      601 cgagggctgc ggccaagagg caggcgccga agacgatgcc gtcacctgca agtagcgaga
      661 caagtaggta gagtagggcc caccatgggg cgaggtccat cgcgctcgtg atgagaaagt
      721 tgttcggcca gtagcggcgg atgatcacca gccagcggtg catctgcgtc caccaccgcc
      781 caaaggtgta gtgcgggtgg gagacgcgag ccggctgctt gagcagatgg atgtggtagc
      841 cttcctggcg caacgcccgg cctaacggga ggtcgtcggt gctgtggcgt ccgaaccgct
      901 ccaacccccc aacgcgctcg atcgcttccc tcctcgtcac catcgtggta cctacggccc
      961 cgtcgaacgc ccggaacagg cacatcgacg cgagacgtag gacaaacggg tttgccgaga
     1021 aggccataag cgctgccgcc cagtcccgtg cccccacgta cgccggcgcg ccgaacccaa
     1081 gccccacctc cgggacctcg aggcacgtaa ccgtttccct caaaaagttc ggcgggacct
     1141 gagcgtcgga gtcactgaat acaataacgt cgtacgagct gtgagcgtaa ccggcgatca
     1201 tgttcttcaa cttgcccacc gacctcgcgt cctcagagtc gctgaagatc aggcgtacga
     1261 acctatccgg gtactcctcg atgatcctct ctatcacaag gacggaagga tcatcccgcc
     1321 cctcgacaca gaagacgacc tcgtaatcgc tcgggtagtc ctgctcgcag aagcctcgga
     1381 agttagccaa cgccgactgg tccaccccct tgacgggctt gataacggac acgggcggcg
     1441 cgtactcccc ccttggaaag cggcggtcgt aggacgcgaa gtaataccag gcgaaggcgt
     1501 gcgagagcaa ggtgctgaga agagagagcg ccacgagggc gaggagcata agctgcagga
     1561 cctctacaac cacgcgagac cccgacgccc tcagccaccg gtgggggagg ccagaaagcc
     1621 actccgtacg agatacacaa ggtatttctc gaaggttccg gcgttcactg gcgggcagga
     1681 gatcccggtg ctcgcgagcg ccgccgttgt gttacggcgg tcgaactcgg ggaccgccct
     1741 gaccatcttc ggaccttcta tggtcgcact catggagagt aggggggccg cggagtacat
     1801 cgcgtcctcc cgtaggccct tcacggggtt cagcagctcc gttcgccatc tgtcgtacgg
     1861 gatccgcttg agtgggtagc cgaacgcctc gatccaggaa atgaggttgt ctatcttcac
     1921 ggggtgagag ttggccaggt ggaagcgctt gccgagcgac ccttcttgga gcgacaggcg
     1981 gattatagcc cggctcacgt agtccacagg gaccatgttc aactccgtgt tcacgtcagg
     2041 gacgttgccg aggccgaccc aactcttaat catcttgcac gtgaagtcgt ccttgttcca
     2101 ggcccccgtc cggctgtcac ccgcgatgag ggacggccgg taaacactca cgggcaaccc
     2161 cctcgcacgc gcgatctcca ccagtttctc ggcgacccac tttgtctggg tgtacccgtt
     2221 gtagagggac cccccgtgat caaggtcgtc gtcctcccgg acgacccccg cgtcggacct
     2281 gccgaccagg gggaacactc ctaaggtgga cacgaagtgc acgggcttcg tcccgtgtcg
     2341 ggacgctagc ctcagcacct cttgcgtccc gaggacgttg gtcggcttga gcgcgtcgta
     2401 cgggtagacc cagttcaccg aggcaccgtt gtgatagaca acgtcgatct cgtctgccag
     2461 ctcctcgaac cgctcgtccc cgagcccgag gagcggttcg gagagatccc ctgcgaccgg
     2521 gaagatcctg ggcgaccgct tctcgtccca cagtgcatac gtttcgagcg acccccgaat
     2581 cctcctctcg ccctcctcga cgttgggggc gcgcactaag cagtggatct ccgcacgagt
     2641 gtgccggagg agctcggcga gcaggaacga cccgagaaag cccgtagctc cggtcagcag
     2701 aacgcgttcc ggccgggacg caacctccgt ggagacggta cccgtaggcc gaattctggg
     2761 gtccagtgcg gcctcggcct ccaggttctc cacattgacg gcgacctgtt cgagcagctt
     2821 atcaaggttc ccggactttc ttagtagcag ctgtgcaagg agggcacgtt tctcttttgg
     2881 cgagaggtag gagagtcggt ggctcaccct ctggtctcct gctcttcctc caccagtccg
     2941 cgcagcagag catcgacctc ctcgtccgac agcccttcca cctgagcctc tactagtctt
     3001 tccctggttt cctctggcgc cgaaaccgac gcagcatcgg cggcagtggg tgcatccagc
     3061 ccttcaagca ggtgcgtcgc caattgggag aggctgggac cttggagcag cgctgtcacc
     3121 ggcactgacg tttcgaggtc cgcttccact cggttcttca gctcgacagc catgagggag
     3181 tcgatgccga ggcggttgag gggctggtgg atgtccacct tggaggtagg gcaccttagc
     3241 actgcagcta gttgctccgc aaggaatttc tccgcgagcg ggcggcgctc ctcaggcggc
     3301 gcggcttgta gtttctcctt ggtgaggccg tcccccctgc gcttgtggct cggggagagg
     3361 tttacctccc tggccagctc ggagagcagc ggcgacgagt aggagctgag caggcgggcc
     3421 cagtccacgg cggcggccat cacctgcacc gggttgcgtt cgaggatcca tcccagcagc
     3481 cgtgctcctt gctccggagt gaacgcaacg aggccctggt ttgagaggtg ccgcgcccgg
     3541 tcctttcgga ccgcgaggcc cacctcggcc cacgcccccc agttaatgga tgtggcgggt
     3601 agaccggccg cccgacggta gtgggccagg gcgtccagaa actcgttggc cgcaacgtag
     3661 tttgcctgac ccggggaccc gagcaccgag gcacctgagg agaagagcat aaagaaatct
     3721 aactcgtcgt cctgggttag gcggtggagg ttccaagctc c
//
LOCUS       DATMJA010000162         4127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_435242, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000162 DATMJA010000000
VERSION     DATMJA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435242"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VNT12_03845"
     CDS             complement(<1..457)
                     /locus_tag="VNT12_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI45282.1"
                     /translation="MSERAPLFSILDLDAGDEEAIRQAANLLVEGFRDDWPEAWPTEE
                     EALQEVREALGEGRICRVALGEDGTVLGWIGGIPSYEGKVWELHPLVVRPDLRRRGIG
                     RALVTDLEARVAERGALTLWLGTDDETGMTTLSGVDLYPDVLGHLANIEN"
     gene            complement(454..1008)
                     /locus_tag="VNT12_03850"
     CDS             complement(454..1008)
                     /locus_tag="VNT12_03850"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI45283.1"
                     /translation="MAEGGGWKRLRSERLMETPYFSLRSDRLRLPDGRIKDPYYVIER
                     PDAAIIFPLTVDGEVVLVSQYRPPLETMELGLPAGLVEEGERPEEAARRELSEETGYT
                     GGEWELLGSVASSPSLKDNWAYLFLAGGVGESAAPDPDEHELVEVIRVPVEELLSLVH
                     SGKIVSSSGVAAIMLALERLREAL"
     gene            complement(1001..1393)
                     /locus_tag="VNT12_03855"
     CDS             complement(1001..1393)
                     /locus_tag="VNT12_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45284.1"
                     /translation="MTRRVLAAVEDLLFRSKISETASSLGIEAAFPRNPKRLLEAIHE
                     SPPALLILDLNSARFEPLTLLRSVRSDDATRDVPTVGFLSHVQKDLAVAAREAGCDRV
                     VARSAFTKDLPRILAGHIPDTIDEVGVG"
     gene            1465..3291
                     /locus_tag="VNT12_03860"
     CDS             1465..3291
                     /locus_tag="VNT12_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI45285.1"
                     /translation="MKRLKELRAGLRRTRETLSNFTPFLRPRRWGLLLALGVLLLETV
                     TSLAQPWPLALTIDYVLTDNKPLPELVPDVLADKALLLAGIAFLIVLIFTVTRSVASF
                     RRYLLQKLGQQTVFDMRGALYAKVHALGLDYHGKKRTGDTITRVTSDVKEVRTMLVDS
                     VVEVFSSVTILGGMMVVMLWLDWQLTLLALVTVPFLFLAVKRYRQALVQRMRIVRTRE
                     GAIASVMQEAIIGIRAVKLFAREDDEMERFRTESRESLGASVESAMIEAKFSTVLGIV
                     GGLGTALVVYFGARQVLAGALSLGDLTVFVAYLALFLSPLWALSRQVNQIGTSLVSGE
                     RIIDLLNAEPTVKDLPGAHPAPVFDGRVTFEDVRFAYEDEAGEVLRGVDFDVEAGSRV
                     ALVGVSGAGKTTITSLIARLYDPQEGRILIDGEDIRDFTLKSLRDSVTFVPQDPLLFR
                     ASVAENIAYGRPGAGREEIEAIAELAGADGFIQELPEGYDTVLSERGESLSGGQRQRI
                     SIARAMLRDTPILILDEPQAGLDAEAAAAVEGNWRALTEGRTTFVIAHELRLVRSVDS
                     ILVIEDGSVAETGTHEDLVSSDGLYARLYSLQERDPVQPERG"
     gene            complement(3427..>4127)
                     /locus_tag="VNT12_03865"
     CDS             complement(3427..>4127)
                     /locus_tag="VNT12_03865"
                     /inference="COORDINATES: protein motif:HMM:NF028352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HWI45286.1"
                     /translation="VFVLGLLDAEATGKSFLYRTGPSFVRVRGGIPERLPLEARDLYH
                     ENVGGGHGLVLVGSHVRQTTRQLRAAKKLDGLRAVELSVTRILDPERRTGELDRVVRL
                     VNEMLSKTDVVVYTSREVVRASGDLSGLEVGSAVSAALVDVMRRVDRALKLGFVVAKG
                     GITSSDVVTKGLGVRRAEVAGQMLQGIVSAWILPEDSDYPGLPYVVFPGNVGDEDALA
                     KVIQKLRAGPDQEQ"
BASE COUNT          710 a         1335 c         1328 g          754 t
ORIGIN      
        1 ggttctctat gtttgccagg tgcccgagga catcggggta gagatctacg ccggagaggg
       61 tcgtcatgcc cgtttcatca tcggtgccga gccagagcgt aagggcgccg cgctcagcga
      121 cgcgggcctc caggtcggtg acaagcgcgc gaccgatgcc cctgcgcctg aggtcggggc
      181 gcaccaccag cgggtgcagc tcccacacct tgccttcgta ggagggtatg ccgccgatcc
      241 aaccgagaac cgttccgtcc tcgcccaggg ctacccggca gatccggccc tctccgagag
      301 cttctcgcac ctcctgtagg gcttcctcct ccgtcggcca ggcctccggc cagtcgtccc
      361 tgaatccttc gaccaggagg ttcgccgcct gcctgatggc ctcttcgtca ccggcgtcga
      421 ggtcgaggat cgagaacagc ggcgccctct cgctcacagg gcttcccgca gccgctccag
      481 ggcgagcatg atggccgcga cgccgctgga actgactatc ttgccggaat ggacgaggct
      541 caagagttct tccacaggca cccgaatcac ctccacgagc tcgtgctcgt cggggtctgg
      601 cgctgctgac tccccgacgc cgccggccaa aaagaggtag gcccagttgt ccttgaggct
      661 aggggaggag gccacggagc ccaatagttc ccactcgccg cccgtgtagc ccgtctcctc
      721 agagagctcg cgacgggccg cttcctccgg tctctcgccc tcttcgacga gtcctgctgg
      781 caggccgagt tccatcgttt cgagcggcgg gcggtactgg ctgacgagga cgacctcgcc
      841 gtccacggtg agcgggaaga tgatcgccgc gtcggggcgt tcgatgacgt agtaggggtc
      901 tttgatccta ccgtcaggca gccgcagcct gtccgagcgt aacgagaagt acggggtctc
      961 cataagcctc tcgctacgga ggcgtttcca gcctccgccc tcagccaaca ccgacctcgt
     1021 cgatggtgtc tgggatgtgg ccggcgagta tccgcgggag gtccttggtg aaagcgctgc
     1081 gggcgacgac acggtcgcag ccggcctccc gcgcggcgac ggccaggtct ttctggacgt
     1141 gagagaggaa gccgaccgtg gggacatcgc gtgtcgcgtc gtcggacctt acacttctca
     1201 ggagcgtgag cggctcgaag cgggcggagt tcagatcgag gatgaggaga gcgggcgggg
     1261 actcgtgaat agcttcgagc aaccttttgg gattgcgggg gaaagccgcc tcgataccga
     1321 ggctcgaggc ggtctcggag atcttgctcc tgaacaaaag atcttctacg gccgctagga
     1381 ctcttctcgt catgtgcgcc tccgtgctct agagttgcgt aagctcgcac attctatccg
     1441 cgggcggtgc ggtatcctat acgggtgaaa agattgaagg agcttcgcgc cgggctaagg
     1501 cgcaccaggg agacgctctc caacttcacg ccattcttga ggccccgtcg ctggggactt
     1561 ctgctggcgc tcggcgtgtt gctgctggag acggtgacga gcctcgcgca gccttggccg
     1621 ctcgcgctca ccatcgacta cgtgctaact gacaacaagc cgctgcccga gctagtgcct
     1681 gatgtgctgg ccgacaaagc gctactcctc gccgggatag cattcctcat cgtactcatc
     1741 ttcacggtaa cgaggagcgt cgcctccttc cggcgctacc tgctgcagaa gctggggcag
     1801 cagactgtct tcgacatgcg gggggcgctg tacgcgaaag tccacgccct ggggctcgac
     1861 taccacggca agaagcgcac gggcgacacc ataacccgcg tcaccagcga cgtgaaagag
     1921 gtacgcacca tgctggtcga ctcggtggtc gaggtattct cctcggtcac tatcctggga
     1981 gggatgatgg tcgtcatgct ctggctcgac tggcagctga cgttgctcgc cctcgtcacc
     2041 gtgcccttcc tgttcctcgc cgtcaaacgc tacaggcaag cgctcgtgca gcggatgagg
     2101 atcgtgagga cgagggaggg cgccatcgcc agcgtcatgc aggaggccat aatcgggatc
     2161 agggccgtca agctcttcgc ccgcgaggat gacgagatgg agcgcttcag gaccgagagc
     2221 agggagtccc tcggagccag cgtagagagc gcgatgatag aggcgaagtt cagcaccgtc
     2281 ctcggaatcg tcggaggcct cggtacggcg ctggtggttt acttcggggc gaggcaggtt
     2341 ctggccggcg ccctctcatt gggcgacctt accgtcttcg tggcctacct cgccctgttt
     2401 ctctcgcccc tgtgggccct gagccggcag gtgaaccaga tcggcacctc actcgtctcg
     2461 ggcgagcgca ttatagatct cctgaacgcg gagccgaccg tgaaggatct gccaggagcc
     2521 catcccgcgc ccgtcttcga cggcagggtg accttcgagg acgtgcgctt cgcttacgag
     2581 gatgaggcgg gagaggtcct gcgcggcgtg gacttcgacg tcgaagcggg gagccgggtc
     2641 gcgctcgtcg gggtgagcgg cgcgggcaag accacgatca ccagcctcat cgcccgcctc
     2701 tacgatccgc aggaggggag gatcctcatc gacggggaag acataaggga tttcaccctc
     2761 aagtctttgc gcgactccgt aaccttcgtg ccgcaggacc ccctgctctt cagggcgtcg
     2821 gtggccgaga acatagccta cgggcggccg ggagcgggtc gggaggagat cgaagctata
     2881 gctgagctcg cgggggctga cggctttatc caggagttgc cggaaggcta cgacaccgtg
     2941 ctctccgagc gcggcgagag tctgagcggc ggacagaggc agcgcatctc catcgcgcgg
     3001 gccatgctca gggatacccc tatcctgatc ctggacgagc ctcaggccgg actcgacgcc
     3061 gaggctgccg ccgccgtcga gggtaactgg agggctctta cagaagggcg aaccacgttc
     3121 gtcatcgccc acgagctcag gctggtcagg agcgtagatt cgatcctcgt gatcgaagat
     3181 ggcagcgtcg ccgagacggg cacccatgaa gaccttgtct cctcggacgg cctctatgcg
     3241 aggctctact ctctgcaaga gcgcgatcct gtgcagccgg agagagggtg aacccttgag
     3301 agagctgctg atcctcagcg tggtactgac ggccgtggcg gtcgcgatct acatctcttt
     3361 cgcgtagcga cgcggtagcc gccttggtcg ttcgatcagg tcccttaggt ccgggaaggc
     3421 ggccagtcac tgttcctggt ctggccccgc gcgtagcttc tgaatcacct tcgcgagcgc
     3481 gtcctcgtct ccgacgttgc cggggaagac cacgtaaggc agcccgggat agtcactgtc
     3541 ttcgggcagg atccaggccg atactatccc ctgcagcatc tgaccggcga cctccgcacg
     3601 ccgcacgccc aggcccttgg tgactacatc gctcgaagtt atgccgccct tcgccaccac
     3661 gaacccgagt ttcagagccc ggtccacgcg tcgcatcaca tctaccagcg cggcagagac
     3721 ggcgctcccg acctcgagcc cagagaggtc tccggaggcg cgtacgactt cgcggctcgt
     3781 atacacgacc acgtcggtct tggagagcat ctcgttgacc agtctgacca cgcggtctag
     3841 ctcccctgtc ctcctctcgg ggtcgagtat ccgggtcacg gacaactcca cggcgcgcag
     3901 cccgtccaac ttctttgccg ctcgtagctg ccgggtcgtc tgccgcacgt gcgagccgac
     3961 gaggacgaga ccgtgccctc cgccgacgtt ttcgtggtag aggtcccgcg cctctagcgg
     4021 caatctctcg gggatgccgc ccctcactcg cacgaacgaa ggtccggtac ggtacaggaa
     4081 agacttgcct gtcgcttccg catcgagcag ccccagcacg aagacct
//
LOCUS       DATMJA010000163         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_613100, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000163 DATMJA010000000
VERSION     DATMJA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613100"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VNT12_03870"
     CDS             <1..184
                     /locus_tag="VNT12_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45287.1"
                     /translation="PEAIEETTTLNPGVESPLLVELQADVYFQPEGVPSGAETTAPTP
                     QETTTPTPPAEGTGGG"
     gene            complement(449..1918)
                     /locus_tag="VNT12_03875"
     CDS             complement(449..1918)
                     /locus_tag="VNT12_03875"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWI45288.1"
                     /translation="MEPVRTEERPKAIPAPGVTISSLIEKVSSYGPEGTEELIADAYN
                     AAHAAHRGQTRKSGEPFVYHPLATADVLAELRLDPTTIAAALLHDVLEDSGVTKEELS
                     EHFGDEVAEIVDGVTKLKRLPSGNLEEAQAESLRKMIVAMSRDVRVIIIKLADRLHNM
                     RTLAYLKRETQLRKATETLEIYAPLAHRLGIYSIKWELEDLSFATLHPRRYEEIKSLV
                     AARRGDREAFINGTAAELLRHLRESGIEAEVHGRVKHFYSIYNKMVRRNKEFNEIYDL
                     AGLRVVVDGVRDCYGTLGVIHSIWKPIPGRFKDYIAMPKFNMYQSLHTTVMSNEGKLL
                     EIQIRTAEMDTTAEYGIAAHWMYKHGLTDRQVDRMTWLKSMMEWQKETTDASDFMESL
                     KGELVAEEVFVFTPKGDVVSLPSGATPIDFAYHVHTEVGHRTIGARVNDRIVPLDSEL
                     VSGDRVEVITGKNSGPSRDWLAVVQSGRARNKIRGSLLR"
     gene            complement(2076..>3617)
                     /locus_tag="VNT12_03880"
     CDS             complement(2076..>3617)
                     /locus_tag="VNT12_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HWI45289.1"
                     /translation="KGLQEFLAPNFGVRLRRTLDDLGPTFVKFGQVLSTRSDILPEGV
                     LSELQKLQDTATPMPAGVAQEIIEGELGAPVDELFASFDPVPLGSASIGQVHRAVLRG
                     GETVAIKVQRPEAPGRVGGDLELMRDFAALLDRRFGRRLFVDVRGLVAEFEVVVRREL
                     DYSAEAENARRFAANFAGTQVIIPGVYLGLSTSRVLTEEYIEGTRFRDIRPLVLSPSE
                     RRRVASMGAEAIFKMAFEDGFFHGDPHPSNLLLTPGGDLALLDFGMVGYMSHGDIEAL
                     SRLFVAVIQRDADAALRALERLGVSYATEVRGDLIRDLREFLNKYSGLSVGEVTLGQA
                     LSELVALARRYRLRMPPVFPLLTKALVTAEGVARSIDPTINVYEVARPYAQRLLSGRY
                     SPEAVIEVAEERALEYARYIEDYPEQIRLLLTEIADGELEVQLQHGGLDELFGSVDVL
                     ANRLVFAVVTAALFVGSSMLAAFYVAGPQVPLLGAPLISFLGFTLSIIQTFFLLTIIF
                     RSGRL"
BASE COUNT          673 a         1118 c         1144 g          682 t
ORIGIN      
        1 gcctgaggct atagaggaga ctacgacgct gaatccgggc gtagagtcac ccctgttggt
       61 ggagctacag gccgacgtct acttccaacc ggagggcgtg ccatccggtg ctgagaccac
      121 ggcgccgacc ccccaggaga ctaccacccc gacaccgccc gccgagggga ccggtggtgg
      181 ataggttgcg gcgggtcgga cgtcgcgtgg ccgaactcgg taggagtttc gtggatacct
      241 tcctgcgtga caacaggttg ctgccgccgg ttctggccct gctcgcgttg ttcgtcttcg
      301 cctgggtcct ggcgggcgtg ttcgtcggca gaaccgaaga tcagaagcct gtcgcgaaca
      361 ggtccgaggt cgcccaggct ggcgatgcag ggggttcgga gcctgccgct ccccaggtag
      421 ataaccccaa cgtggattcc tatgcagcct accgcagtaa ggatcccctt atcttgttcc
      481 tcgcgcgccc actctggacc acggccagcc agtcgcggct aggtcccgag ttcttacccg
      541 tgattacctc gaccctgtca ccggagacta gctcggagtc gagggggacg atacggtcgt
      601 tgaccctggc ccctatcgtg cggtggccaa cctcagtgtg gacgtggtag gcgaagtcta
      661 tgggcgtcgc cccgctggga aggctgacca catcgccctt cggcgtaaag acgaagacct
      721 cctcggcgac gagctcaccc ttcaaagatt ccataaagtc gctggcgtcg gtcgtctcct
      781 tctgccactc catcatgctt ttgagccagg tcatgcggtc cacctgacga tcggtaaggc
      841 catgcttgta catccagtgg gccgcgatgc cgtactccgc cgttgtgtcc atctcggcgg
      901 tccggatctg tatctcaagc agcttccctt cgttggacat gacggtcgtg tgaagcgact
      961 ggtacatgtt gaacttgggc atcgctatgt agtccttgaa gcggccaggg ataggcttcc
     1021 agatcgagtg tatgacgcca agggtgccgt agcagtcgcg cacgccgtcg acgacgaccc
     1081 tgaggccggc gaggtcgtag atctcgttga actccttgtt gcgacggacc atcttgttgt
     1141 agatggagta gaagtgcttg accctcccgt ggacctcggc ttctatcccc gactcccgca
     1201 ggtgccgcag gagctcggct gccgtcccgt tgataaacgc ctccctgtcc ccgcgcctgg
     1261 cggcgacgag gctctttatc tcctcgtagc gccgcgggtg tagggtggcg aaagagaggt
     1321 cttcgagctc ccacttgatc gagtagatcc caagcctgtg cgcgaggggg gcgtagatct
     1381 ccagcgtctc cgtcgccttc ctcagttggg tctcgcgctt gaggtaggcg agggtcctca
     1441 tgttgtgcag cctgtcggcg agcttgatga tgatcacacg cacgtcgcgg ctcatggcca
     1501 cgatcatctt cctcaaagac tcggcctgcg cctcctccag gttgcctgaa ggcagccgct
     1561 tgagcttggt gacaccgtcg actatttcgg ccacttcgtc accgaagtgt tccgacaact
     1621 cctctttggt tacgccggaa tcctcgagca catcgtgcag caacgccgcg gcgatggtcg
     1681 taggatcgag gcgcaactcg gcgaggacgt cggccgtcgc gagcgggtgg tagacgaaag
     1741 gttcgccgct cttgcgagtc tggcccctgt gcgccgcgtg ggccgcgttg taggcgtcgg
     1801 cgatcaactc ctccgtcccc tcgggtccgt aggacgatac cttctcgata agagaagata
     1861 tggttacgcc aggcgccggt atggcctttg ggcgttcctc tgtacgaacg ggttccatag
     1921 cgaaattata ccagtaaggc aataggtatc aggtaacaga caccagcgag agagctcgac
     1981 accaggccac ctttaggcag cgggtctaca tacctctcaa ccccgacccc tgaaatgctg
     2041 acaagcggaa gccgaagcct ggagcgtgct gaaacctaca gccttccgga tctgaaaata
     2101 atcgtgagga ggaagaaggt ctgaataatg gagagcgtaa atccgaggaa gctgatcagg
     2161 ggtgcgccga gaaggggcac ctgcggacct gcgacgtaga aagcggcgag catcgaggac
     2221 ccgacgaaca gggcggcggt aacgacggcg aagaccagcc ggttggcgag aacgtcaacg
     2281 ctgccgaaaa gttcgtcgag gccgccgtgc tggagttgca cttcgagctc accgtcggcg
     2341 atctcggtaa gtagaagtct gatctgctcg ggatagtcct cgatgtagcg ggcatactcc
     2401 agcgcacgct cctcggcgac ctcgatcacg gcctctggac tgtagcgtcc ggagaggagc
     2461 ctctgtgcgt agggcctggc gacctcgtag acgttgatag tcgggtctat ggagcgggcg
     2521 actccttctg cggtcacgag cgccttggtg agcagaggga agacgggcgg catcctgagc
     2581 ctgtagcggc gcgcgagcgc gacgagctct gagagcgctt ggccaagtgt gacctcgccc
     2641 actgagagcc cggagtactt gttgaggaac tcccgcaggt cccgaatgag gtcgccgcgc
     2701 acctcggtcg cgtagcttac gccgagccgt tcgagggccc gcagggcggc gtcggcgtca
     2761 cgctgtatca cggctacaaa gagcctcgag agcgcctcga tgtcgccatg gctcatgtag
     2821 ccgaccatgc cgaaatctag tagggctagg tccccgccag gtgtaaggag caggttgctc
     2881 gggtgggggt ccccgtggaa gaatccgtcc tcgaaagcca tcttgaagat cgcctcggcc
     2941 cccatagagg cgacgcgccg ccgctcagag ggagagagca caagcggacg gatgtccctg
     3001 aacctggtcc cttcgatgta ctcctcagtc agcaccctcg aggtcgagag tcccaggtag
     3061 accccaggga taattacctg cgttcccgcg aagttggcgg cgaagcggcg ggcgttctcc
     3121 gcctcggcac tgtaatcgag ctcgcgccta accacgacct cgaactcggc cacaaggccg
     3181 cgcacgtcta caaagagcct gcgcccgaac ctccgatcga gcaaagcggc gaagtcacgc
     3241 atgagctcga ggtcgccccc gaccctgccg ggcgcctcgg gccgctgcac cttgatggcc
     3301 acggtctccc cgccacgcag caccgccctg tggacctgcc ctatgctcgc cgagccgagc
     3361 ggcacagggt cgaaagaggc gaacaactca tcgacaggag ccccgagctc accctcgatt
     3421 atctcctggg cgacccctgc cggcatgggc gtggccgtgt cctggagctt ctggagctcg
     3481 gagagtactc cttcaggcag tatgtccgag cgtgtggaga ggacctgccc gaacttgacg
     3541 aaggtcggcc ccagatcgtc gagggtgcgc cgcaacctga caccgaagtt gggcgccagg
     3601 aactcctgca ggccttt
//
LOCUS       DATMJA010000164         4680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_376246, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000164 DATMJA010000000
VERSION     DATMJA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376246"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1045)
                     /locus_tag="VNT12_03885"
     CDS             complement(134..1045)
                     /locus_tag="VNT12_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI45290.1"
                     /translation="MIGAPLSRLPGGLYVDLGKGDHRSSVFLAGSGRSGTTWLSEIIN
                     HRRGYRYVFEPFNPRVVGPFGHFRTKQYLRPDDQREEYLEPASLALTGGLRDPWTDRF
                     NGRVVARRRLIKDIRANLLLGWMRANFPGMPIILLLRHPCAVVASRLALGWRDNLSET
                     MEQRELVEDFLLPMEAEIRAAKGDFERHLFLWCIDNYVPLKQFGPQEVHLTFYENLLA
                     RPESELQSMFAFLGDDFDDRVYRRMRRPSPLSRKNTPCPSTEGWRTRLSAHQLERTME
                     ILRLFGLDHLYTERTMPDPSFTLMDGV"
     gene            complement(1157..2800)
                     /locus_tag="VNT12_03890"
     CDS             complement(1157..2800)
                     /locus_tag="VNT12_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWI45291.1"
                     /translation="MRKVQPPGDASERFSGTERKGERDKYRRPGRLLAVFKGLVMLAL
                     LAATAYGMLDAGLYRDELWLPVAAGVLALLFVTVFVRGFYWDLPTVGILLVGLLAALV
                     AVKGLSMVWTISETETIKETLRSSMYLAAFLMALAALTSGRQVGSLIDASVFMVAAVA
                     GYGFLQKISPITYPVRSLDGVRMDSTLQYSNTTAVILGMGVVLALARMSTLRNFVMRG
                     LYAAITLSFFVALYLTVSRGGIGSLGIGLIVLFIITSGRLRMFTDLILLSLPGAWLWW
                     QMQSLPGLLGTGVSRQQKIADGTLFRNDLILALVVAFVLQAAFTILMNRYELSPVLRR
                     RLGILVVAGGVLAMTLGAFVVVDGYGGPVQAYSTLLGSANEKGNAGQRLASLDISSRG
                     DYWRVAWAAWKERPLTGTGAGTFQYTWLEDRPGLKGVKQVHNVYLEQGTETGLFAFLA
                     LLGFVAVLVGYTGRAVWRSDPQGERRLLLAGLCSALIVYLVSSAFEWHWYLPASTLFF
                     FILAAVAVKFASREDWGAAEAPSADSGAGRPHTPVEAEG"
     gene            complement(2838..3470)
                     /locus_tag="VNT12_03895"
     CDS             complement(2838..3470)
                     /locus_tag="VNT12_03895"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HWI45292.1"
                     /translation="MRLGAISDAGARGLLDPDSPVRRHYEDLAGNLLRVGGTGSVVVT
                     SPEPGNGRTSICLGLGAALAGMGRRAAVVDCNLENSQLHRMIGEPNFVGLTSGLDDSK
                     PLEHFGYEVIPGLLVVPTGPILLDPTSLLEDEGVPKAVRGLQKSRDLVLLDAPVAARV
                     LRSPNLLGGFDGVLLVVHASRTSKSVARETTDDLLEAGANLLGVVLNGCS"
     gene            complement(3472..4200)
                     /locus_tag="VNT12_03900"
     CDS             complement(3472..4200)
                     /locus_tag="VNT12_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWI45293.1"
                     /translation="MASTSGRGWRDRAREDPSLSEILYVLQRRRLLIVGVALVLAGIA
                     LLFGLFREPVYTAEAKVSVRPQEEPDGEEAMLAFLEEVRGAVVTQEMLSEVRRRAGWE
                     AGPKAFSERLDPRTVVDTDGGPGLQIRFLGDEPGQAALAANAYAELFVEGVEDLGDRP
                     LADGVPVAGAVVEQRALPGDYSPRPLIYAAIAAGAGLLIGGAAALILEARASGWRGVR
                     DAELTLRVPVLGTIPDYSSKKTEG"
     gene            complement(4187..>4680)
                     /locus_tag="VNT12_03905"
     CDS             complement(4187..>4680)
                     /locus_tag="VNT12_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI45294.1"
                     /translation="LRLGMGDLDAAVEGYRDVLQLNPNAVSARSSLAQTLAMQGKLQE
                     AKEAYEALEGETNISYQDRYDLGRIQVRTGEPLEGVKNIRRARRMAAAELNRPRTRVG
                     ERQLLVSMDLATADAFVVQGRYGQARRILARSRSPQAPGLLELLNLDPAAYREQVINS
                     GIY"
BASE COUNT          869 a         1559 c         1448 g          804 t
ORIGIN      
        1 gtggtctcga cgaagacggt aggatagccg gcgccggcga agagggtggc gagcgtctgg
       61 tgcctttgcc agaggaagtc ccaccttaca ccggagagga tcaccaccgg cggcttgtgc
      121 acgagagagg ctcctacact ccgtccatca gggtgaaaga agggtctggc atggtccttt
      181 cggtgtagag gtggtcgagg ccgaagaggc gcaggatctc catcgttctc tccagctggt
      241 gggcgctcag gcgggtacgc cacccttccg tggaaggaca gggcgtattc ttccggctca
      301 ggggggaagg gcgtctcatt cttcggtaga cccggtcgtc gaaatcatcg cccaggaagg
      361 cgaacatgct ctgtagttcc gattccggac gcgcgagcag attctcgtag aaggtgaggt
      421 gcacctcctg tggcccgaac tgtttgaggg ggacgtagtt gtctatgcac cagaggaaga
      481 ggtggcgttc gaagtcgcct ttggctgccc ggatctccgc ctccatgggg agaaggaagt
      541 cctctaccaa ctcccgctgc tccatcgtct ccgagaggtt gtccctccac ccgagcgcga
      601 gcctggatgc cacgacggcg caaggatgcc tgagcagtag aatgatcggc attccgggga
      661 aattcgcgcg catccagccg aggagcaagt tggcccttat gtccttgatc agacgccgcc
      721 tcgcgacgac gcgcccgttg aagcggtcgg tccaggggtc cctgagcccg cccgtgagcg
      781 cgaggctggc cggctccaga tattcctccc tctggtcgtc tggtctcagg tactgtttgg
      841 tcctgaagtg cccgaatggg ccgaccactc tcgggttgaa aggttcgaag acgtaacgat
      901 acccccttct gtggttgatg atctctgaga gccacgttgt cccgctccgc ccgctccccg
      961 ccagaaagac ggagcttctg tggtcccctt ttcccaggtc gacgtagaga ccgcctggca
     1021 gccgtgagag aggcgcgcct atcatcgtac ggcctccccc ctgagggttc ccatcaacgc
     1081 gtccgcgaag gaggcaagga accggcgatc gtagtcgctc tccgacctgg gatagccaac
     1141 gctcatcggg ccgatgctat ccttcggctt cgacgggggt gtggggccgc ccagccccac
     1201 tgtcggcgct gggagcctcg gcggcacccc agtcctccct cgaggcgaac ttcactgcga
     1261 cggcggcgag gataaagaag aagagcgtcg aagccgggag gtaccaatgc cactcgaacg
     1321 ccgaggagac gaggtagaca atgagcgcgg agcatagccc ggccagaagt agccggcgtt
     1381 ctccctgcgg atccgaccgc cagacggctc tccctgtgta gccgacgagg accgccacaa
     1441 acccaagcag ggccaaaaag gcgaacaagc cggtctcggt gccctgctcg agatatacgt
     1501 tgtggacctg tttgacgccc ttgagccccg gtctgtcctc cagccacgtg tactggaagg
     1561 tccccgcccc cgtacctgtg agggggcgct ccttccacgc cgcccaggcg accctccagt
     1621 agtctcccct ggagcttata tctagcgagg cgagcctctg gccagcgtta cctttctcgt
     1681 tcgcgctgcc gaggagcgtc gagtaggcct gcactggacc gccgtacccg tcgacgacca
     1741 cgaaagcgcc cagggtcatc gcaagcaccc ctccggccac gacgaggatc ccgagtctcc
     1801 gcctgagaac cggtgacagc tcgtagcggt tcatcaggat ggtgaaggcg gcctggagca
     1861 caaaggccac gacgagggcg aggatcagat cgttcctaaa cagcgtcccg tcggcgatct
     1921 tctgctgacg tgagacgccg gtcccgagta acccagggag gctctgcatc tgccaccaga
     1981 gccaggcgcc tggcaacgag aggaggataa ggtcggtgaa catccgcagg cgaccgctcg
     2041 ttatgatgaa gaggacgatg agtcctattc ccagcgaccc gatgccgccg cgagagaccg
     2101 tgaggtagag cgcgacgaaa aaggagagcg tgatggcggc gtagagtccc ctcattacga
     2161 aattacgcag cgtactcatc cgcgccaggg ccagtaccac ccccatcccc aggatgacgg
     2221 cggtggtgtt agagtactgc agggtcgagt ccatccttac cccatccagg gagcgaaccg
     2281 ggtacgtgat cgggctgatc ttctgtaaga atccgtagcc tgcgaccgcc gcgaccatga
     2341 agaccgaggc gtcgatgagc gagccgacct ggcgccctga ggtgagcgcg gccagtgcca
     2401 taaggaaggc cgccaggtac atagacgagc gcaaggtctc cttgatggtc tccgtctcgc
     2461 ttatcgtcca gaccatggag aggcccttta cggcgacgag cgcagccagc agcccgacca
     2521 gcaggatgcc gacggtgggc agatcccaat agaaaccgcg cacgaacacc gttacgaaca
     2581 gcagggccag aacgcccgcg gcgacaggca accagagctc gtccctgtag aggccggcgt
     2641 ccagcatccc gtaggccgtt gcggccagga gcgccagcat gacaagtccc ttgaagacgg
     2701 cgagcagccg gcctggacgg cggtacttgt ccctctcccc tttgcgttcc gtgccggaaa
     2761 agcgctcgct ggcgtcgccg gggggctgga ctttcctcac gccttggccc ctgggcgagt
     2821 ccctcggcga tcgcgcatca ggaacatccg ttcaacacca ccccgagcag gttggcacct
     2881 gcttccagaa ggtcgtccgt cgtctcgcgg gcgacgctct tggaagtccg ggaggcgtgg
     2941 acgacgagca ggaccccgtc aaagccgcca aggagattcg gggaccgtag cacccgcgcc
     3001 gccaccggcg cgtcgagcag cacgaggtcc cggctcttct gcagacctcg taccgccttc
     3061 gggaccccct catcctccag tagagaggtg gggtccaaca gaatgggacc ggtcgggacg
     3121 accagaagtc ccgggatgac ctcataccca aagtgctcca ggggtttact gtcgtcgaga
     3181 ccgctggtaa gcccgacgaa gttcggttcg ccgatcatcc tgtgcaactg cgagttttcg
     3241 aggttgcagt ccacgacggc ggcccgccgt cccatgccgg ccagggcggc tccgaggccg
     3301 aggcagatgc tggtccgccc gttgccaggc tcgggactgg tgacgacgac actacccgtc
     3361 ccccccactc tcaggaggtt gcccgccaga tcctcgtagt gacgcctcac tggtgagtcg
     3421 gggtcgagca accccctggc accagcatcg cttatggccc cgagcctcac gtcaaccctc
     3481 ggttttcttc gaagagtagt ccggtatggt ccccagcacg ggcaccctca gcgttaactc
     3541 ggcgtcgcgt acgccccgcc acccgctggc ccgcgcctca agtatcaaag cggcggcacc
     3601 ccctatcagc aagcctgccc ctgcggcgat ggccgcgtag atcagaggcc gaggactgta
     3661 gtcccccggc agggctctct gctctacgac ggcccccgca accggtacgc cgtcggcgag
     3721 cggcctgtcg ccgaggtcct cgactccctc gacgaataac tcagcgtaag cattcgccgc
     3781 cagggccgcc tgcccgggct cgtcgccgag gaatcgtatc tgtaaccctg gcccgccgtc
     3841 cgtatcaacg accgtcctcg ggtcgagccg ttctgagaac gccttcggcc cggcttccca
     3901 gccagcccgg cgccttacct cgcttaacat ctcctgcgta acgacggcgc cacgcacttc
     3961 ttcgaggaag gcaagcatgg cctcctcacc gtccggctcc tcctgcggcc tgacgctcac
     4021 cttcgcctcg gcggtgtata ccggctcgcg gaagagcccg aagagcaggg ctatgccagc
     4081 aaggaccagc gcgaccccga cgattagcag acgccgccgc tgcaaaacgt agaggatctc
     4141 agagagcgag ggatcttccc tcgccctgtc ccgccagccc cggccgctag tagatgccac
     4201 tgttgatgac ctgctcgcga taggccgcgg ggtccaggtt cagtaactcc agaagacccg
     4261 gtgcctgggg ggaccggctc cgcgcgagaa tcctgcgcgc ctgcccatac cgaccctgaa
     4321 cgacgaacgc atcggcggtg gcgagatcca tagagaccag gagctgccgc tctcctaccc
     4381 gtgtacgcgg cctgttcagt tcggccgccg ccatgcgtct cgcacgcctt atattcttca
     4441 caccttcgag cggctcgccg gttcgtacct ggatcctgcc aagatcgtag cggtcctggt
     4501 agctgatgtt cgtctctccc tcgagcgcct cgtacgcctc tttggcttcc tgcagctttc
     4561 cctgcatcgc gagcgtctgc gcgagagaac tgcgggccga aacggcgttc ggattgagct
     4621 gaagcacatc acggtagccc tcgaccgcag cgtccagatc tcccataccg agccggagat
//
LOCUS       DATMJA010000165         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_140015, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000165 DATMJA010000000
VERSION     DATMJA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140015"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VNT12_03910"
     CDS             complement(<1..533)
                     /locus_tag="VNT12_03910"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWI45295.1"
                     /translation="MPPIIGITATLKEDVDAVAERPLGKFVRADLDYIDGVAGAGGAP
                     VVLPPTGDEGAAEAVIHSLDGLLLSGGSDLDPGYYGEEPVPELGVTLPERDAFEMALV
                     RLALRRGMPVFGICRGMQVLNVALGGTLYQDLPSQWEWDVLKHRQDTPKWQPAHEVRV
                     REGSYISEVMGCEVVKV"
     gene            complement(695..>2008)
                     /locus_tag="VNT12_03915"
     CDS             complement(695..>2008)
                     /locus_tag="VNT12_03915"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HWI45296.1"
                     /translation="VVEVEQKSATRRPPTPFNTTAFLTAAANIGISPSRAARIAEDLY
                     TDGFISYPRTDNTVYPRSLDLREVLGYLGSVEGVGQHAERMLSQEKLSPTRGKKQTTD
                     HPPIYPTGYASKKSMRDDRWKIYQLVVRRFLATLSAPAKTLRTTLRFDSGGEPLVTGG
                     TVVTEEGWLGVYPYSRRADEEIPNLSEGDIVQVIGKEILSKETQPPGRYGAGRLINVM
                     EDLGLGTKATRPNIIQNLYDRGYVHDDPLVPTEKGMAVAQALTYFASEIASHEMTAEL
                     ERSMDEISDGKVSKDSVVDSSRDVLRRVYDHLQNSETEFADIVWEGIRTDETIGKCPE
                     SGHDLIIRRNRKSRKRFVGCTGYPDCRVTYPLPQRGEIIPLGTRCDACGSPEIKVLGR
                     KRPWVTCINMDCPKKQEQREAAEKAKEGGEGSSTQSAQELQKSTT"
BASE COUNT          357 a          649 c          592 g          410 t
ORIGIN      
        1 ttgaccttta cgacctcgca gcccatgacc tcggagatgt aggacccttc cctgaccctt
       61 acctcgtgcg ctggctgcca cttgggagtg tcctgcctgt gcttgagcac atcccactcc
      121 cactgtgaag gaaggtcctg atacagcgta ccgccaaggg ctacgttcag gacctgcatc
      181 ccccggcaga ttccgaagac cggcatccca cgccgcaagg ctaacctcac cagcgccatc
      241 tcgaaagcat cccgctcggg gagcgttacc ccgagctcgg gtaccggctc ctcgccgtaa
      301 tagccgggat ccaggtcgct cccgccactc aagagcaacc catcgagact gtggatcacc
      361 gcctcagcag ccccctcatc ccctgtcggc ggtaagacca cgggcgcacc cccagccccc
      421 gcgacgccat cgatatagtc gaggtcggcc ctcacgaact tgcctaacgg acgctcagca
      481 accgcgtcca catcctcttt caacgtcgcc gttatgccaa tgatcggggg caagctctac
      541 tcacctttta acaatatctt aactcaacta tatacggcag aataccagac cacaacgatg
      601 atggcgagga gaataaggta accggtagcg cggatatacg ccgaaggtcg atagaaatgg
      661 ggctcatcgg ccaggtccag gtcgagcctg ctctctatgt ggtggacttc tgaagttcct
      721 gagcgctctg agtcgatgag ccctcacccc cctctttcgc cttctcggcg gcctcacgct
      781 gttcctgttt cttaggacag tccatattaa tacatgtaac ccagggacgc ttcctgccaa
      841 gcacctttat ctctggagag ccgcaggcgt cgcaccgggt accgaggggt atgatctcac
      901 cccgctgcgg aagcgggtag gtgacccggc agtcgggata gcccgtgcag ccgacaaagc
      961 gcttgcggct cttacggttg cggcggatga tcaggtcgtg cccgctctcg gggcacttgc
     1021 ctatggtctc gtctgtcctt atgccctccc acacgatgtc cgcgaactcg gtctcggagt
     1081 tctggagatg gtcgtagacc ctgcgaagca catctcgcga cgagtcgacg acggagtcct
     1141 tgctgacctt gccgtccgag atctcatcca tgctccgctc cagttcggcg gtcatctcgt
     1201 gcgaggcgat ctcggaggcg aagtacgtga gggcctgcgc cacggccata cccttttcgg
     1261 tagggaccag cgggtcgtcg tggacgtagc cgcggtcgta gaggttctgg attatgttcg
     1321 ggcgcgtcgc cttcgtgccg aggccaaggt cttccatcac gtttatgagg cggcccgcgc
     1381 cgtagcgacc aggaggctgg gtctctttgg agaggatctc tttaccgatg acctgcacga
     1441 tgtcgccctc ggagaggtta gggatctcct cgtctgcgcg cctgctgtaa gggtagacgc
     1501 cgagccaacc ctcctccgtg acgaccgtcc cgcccgtgac gagcggttcg ccgcccgagt
     1561 cgaagcgcag cgtcgtgcgc agcgtcttcg ccggggctga gagggtggct aggaagcgcc
     1621 ttacaacgag ctggtagatc ttccaccggt cgtcgcgcat cgacttcttc gacgcgtagc
     1681 cggtcgggta gatcgggggg tggtccgtag tctgcttctt cccgcgcgtg ggcgagagct
     1741 tctcctggga gagcatcctc tcggcgtgct gcccgactcc ctcgacgcta cccaggtacc
     1801 cgagcacctc gcgcaggtcg agcgatcgcg ggtaaacggt gttgtccgtc ctgggataag
     1861 agatgaagcc atccgtgtac aggtcctcgg ctatacgggc cgccctagaa gggctgatcc
     1921 caatgttcgc ggccgcggtc aggaaggctg tcgtgttgaa cggcgttggc ggcctgcggg
     1981 tcgcggactt ctgctctacc tcaacgac
//
LOCUS       DATMJA010000166         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_258103, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000166 DATMJA010000000
VERSION     DATMJA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258103"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VNT12_03920"
     CDS             complement(<1..1399)
                     /locus_tag="VNT12_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI45297.1"
                     /translation="MQRIVRWCLKNKSVVILATILLIGSGVYATTRLNQELLPDISFP
                     IITISTPVAGAGPDLVDEQVTQPVEDAINGVQGIEAVRSTSSQGFSVVVVEFGLDADT
                     EEAESDLQAALDGIQLPSQAGDPEVKSQSASQFPILSLSLAAEDQDLADLTSYAEDEV
                     VSSIEEVDGVASVDVIGGAEKRIQVDLDPDKVKEKGLSTDAVVGALSGAEVDAPVGSV
                     TVNGRETPVNTTSELGGISALEALPVGVDGGDTGRAPSAVPADIPGAALPSASAPPGG
                     APAAAPAGSAVGTTGSSAAAAEPVLLGDVAEVREVGSGLSGISRTNGEPSLGLNVIKE
                     TDANTVEVAEEVERVLDDVRNRIGRDQVVIVANAATDVEESVNGLVEEALVGAVLAIL
                     VIFAFLRSMRATLVTAVSLPTSVLAALLFSWVDNLTLNIITLAGLTIAVGRVVDDAIV
                     VLENSYRYVQSGYEPE"
     gene            complement(1399..1872)
                     /locus_tag="VNT12_03925"
     CDS             complement(1399..1872)
                     /locus_tag="VNT12_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWI45298.1"
                     /translation="MDRERLVDETLMLLPTLMRLVERPSPVEMGEISRRGVATDVQVS
                     PGHIQVLIALARGPRSVGQLAEELGVSPPAATQLVDKLAEHGMVDRHNDPEDRRVVLV
                     DYVAGMHEVARRIVGDRRRPLHDAMSKMTDEEALAFVKGLRLLAQIFGAATGEEI"
     gene            2006..>2476
                     /locus_tag="VNT12_03930"
     CDS             2006..>2476
                     /locus_tag="VNT12_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI45299.1"
                     /translation="MKLRTALLAIGGISGGLALYNRRLERGGGRLPNRLGGETRYYRW
                     RGEDLAYSVAGEGEPLLLVHGVYAGASSFEFRKNFEELSKSFRVYALDLLGCGMSERL
                     SRRYEPEDVASQVEDFVREEIRDSAHLVASSLSAALVVPAFVRNPRLFQKVVLIC"
BASE COUNT          443 a          805 c          769 g          459 t
ORIGIN      
        1 cctccggctc gtagccgctt tgcacgtagc gataggagtt ctcgagcacc acgatcgcgt
       61 cgtcgaccac cctgcccacg gctatcgtca ggcccgcgag cgtgatgatg ttcagcgtca
      121 ggttatctac ccaggagaag aggagggcgg cgagcacaga ggtaggcaga gagaccgcgg
      181 tgaccagggt tgcgcgcata gagcgcagga aggcgaagat caccagtatg gctagtacgg
      241 ccccgacgag cgcctcctcg acgagcccat ttaccgactc ctcgacgtcg gtcgcggcgt
      301 tggccacgat cacgacctga tcccgcccga tcctattgcg cacgtcatcg agtactctct
      361 cgacctcttc agcgacctcc acggtgttgg cgtcggtctc cttgatcacg ttgaggccca
      421 ggctcggctc cccgttggta cgcgagatcc ccgataggcc cgagcctacc tcgcgcacct
      481 cggccacatc ccctagcagc accggctctg ccgccgcagc ggaagatccc gtcgtaccca
      541 ccgcgctacc ggcgggggcg gcggccggag cgccacctgg aggagctgaa gcactgggta
      601 aggcggcgcc tggaatatcc gcgggcacag ctgaaggggc cctgccggta tcgccgccgt
      661 ccacgccgac gggaagggct tcgagggcac ttatccctcc gagttcgctg gtggtgttta
      721 cgggagtctc gcgtccgttt acggtgacgc tacccacagg agcatccacc tccgccccgc
      781 tcagcgcgcc gaccacggcg tcggtcgaga ggcccttctc tttcactttg tcggggtcga
      841 gatccacctg gatacgtttc tcggcgcccc cgatcacatc gacgctcgcc acgccgtcga
      901 cctcctcgat ggaggagacg acctcgtcct cggcgtagct tgtgaggtcg gcgagatcct
      961 ggtcctccgc agccagcgag agcgacagga tggggaactg cgaagccgac tggctcttga
     1021 cctcgggatc tcctgcctgc gaaggaagct gtatgccgtc gagggccgcc tggaggtcgg
     1081 actcggcctc ctctgtgtcg gcgtcgaggc cgaactcgac gaccacgacg gagaatccct
     1141 gcgaggaggt ggaccggacc gcttctatcc cctgcacccc gtttatggca tcctcgacag
     1201 gctgcgttac ctgctcgtca accaaatcgg gtcctgcgcc ggccacgggg gtcgagatgg
     1261 ttatgatcgg gaaggagatg tcgggcaaga gctcctggtt gaggcgcgtc gtcgcataga
     1321 ccccgctccc gatgagcagg atcgtcgcca ggatgactac ggacttgttc tttagacacc
     1381 aacggacgat cctctgcatc agatctcctc ccccgtagcg gcgccgaaga tctgggccaa
     1441 cagcctcagg cccttgacga aggccagagc ctcctcgtcc gtcatcttgc tcatggcatc
     1501 atgtagcgga cgacgacggt cccccacgat gcgtctggct acctcgtgca tgcctgccac
     1561 gtagtcaacg agaacgaccc gcctgtcctc aggatcgttg tggcggtcta ccatcccatg
     1621 ctcggccagc ttgtccacaa gctgcgtcgc agccggcggc gaaaccccca actcctcggc
     1681 gagctgcccg acggagcgcg gcccacgcgc caacgctatc aagacctgaa tgtgcccagg
     1741 cgaaacctga acgtccgtcg ccaccccgcg acgcgagatc tcacccatct ccacaggact
     1801 gggccgctcg actaggcgca tcaaagtcgg tagcaacatc aaggtctcgt caacgagccg
     1861 ttccctgtcc acgtgaattt tcctacaaat ttaagctgct tatataattt gctcaaatgc
     1921 tatcccaggc tgtgggattg tcaacgagga atctggccga cgttttcggt acgatcctgg
     1981 catcgagcat ttctgaggag gcgggatgaa gttgaggaca gcactcctgg cgatcggggg
     2041 cattagcggg gggttggcgc tctacaaccg gcgcctggag agaggcggtg ggcgactgcc
     2101 gaaccggctc ggcggagaga cgcgctacta ccggtggagg ggtgaggacc tcgcatactc
     2161 ggtggcggga gagggtgaac cgctgctcct ggtgcacggc gtctacgccg gggcgtcctc
     2221 cttcgagttc cgcaagaact tcgaagagtt atccaagagc ttcagggtat acgccttgga
     2281 tctcctcggg tgcggcatgt cggagaggct ttcgcggcgc tacgagcccg aggacgtcgc
     2341 gtcgcaggtc gaggacttcg tgcgggagga gatcagggac tctgcccacc tcgtagccag
     2401 ctcgctatct gcggcgctcg tcgtgccagc cttcgtgcgg aatccgcggc tcttccagaa
     2461 ggttgtcctg atctgt
//
LOCUS       DATMJA010000167         3735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_672904, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000167 DATMJA010000000
VERSION     DATMJA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672904"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VNT12_03935"
     CDS             complement(<1..506)
                     /locus_tag="VNT12_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HWI45300.1"
                     /translation="MIEAQDVLTQYLGRVRGGRLLDAGEEKALARRVRGGDMRARQKL
                     IESNLRLVISIAKKYRGRGVPFEDLIQEGNAGLIRAVEKFDPEMGNRFSTYATWWIRQ
                     AVTRAVADHARTVRLPAHVVDALYRLRRAENALSIELGRDATEEELVERLGVKPEEAR
                     RLREVSQP"
     gene            complement(581..877)
                     /locus_tag="VNT12_03940"
     CDS             complement(581..877)
                     /locus_tag="VNT12_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45301.1"
                     /translation="MSGEAQRKARKRRSALRPDTALVPVGELEHEIYVWSLPTCCLRS
                     LKEMAMSEVGERRRVELDCSCGRSWRITSSLDDRILERFVTHGGPRVGGSYPAA"
     gene            complement(920..1123)
                     /locus_tag="VNT12_03945"
     CDS             complement(920..1123)
                     /locus_tag="VNT12_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45302.1"
                     /translation="MGGSAWIAALGEEVVYEEDLPACCRRSALHFDDSFECPVCGAMW
                     REPLPLQPEEDAFAQREERRGAA"
     gene            complement(1123..1695)
                     /locus_tag="VNT12_03950"
     CDS             complement(1123..1695)
                     /locus_tag="VNT12_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45303.1"
                     /translation="MREEYMIERQGKRFVLYAGLLEEAHSRGLRSIETELLQVPSKEN
                     GEVAIVKAVIRTEEGKFGGIGDASPQNVNRTIAPHLIRMAETRAKARALRDAINVGVT
                     AFEELGGEEEVVESSGTQEREGRPKAQHEEGRGELPATRKQLNYLEALISDVVEDGIG
                     RFEEMVGKPVGELTREEASEWIGRLSGRAA"
     gene            complement(1772..2344)
                     /locus_tag="VNT12_03955"
     CDS             complement(1772..2344)
                     /locus_tag="VNT12_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWI45304.1"
                     /translation="MIEERAHRVFEVAGGDFGGKLMVTRRTGRAVREALEGALEELPK
                     GGILYVDTRGVELMDYSFADEALGILASRSASGEYGDRHLVLVEEDRDLLENVEASLR
                     QRSLAMIRVDEIGAEPGIIGKVPDHLVETLQAIYDAGSITNAALAARLDLNHTACNNR
                     ATRLAKLGLIHRRIETAAPGGRQYTYERIA"
     gene            complement(2440..3354)
                     /locus_tag="VNT12_03960"
     CDS             complement(2440..3354)
                     /locus_tag="VNT12_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45305.1"
                     /translation="MKTQLELNIRSRGAMSGVLTMGSLDPAFAAATSVHGEPVVVDLR
                     TVEFVEPAGLCALAALLEYLSARCEVVDLALAGRDVPAYLERMNFFRSFENRVRTNAD
                     VASLEERRRHNPGTLQELINFHTEEEIPGIIERISEILENQGYRLRERVAICSTLSEV
                     CANAAEHGVSSFGAYAAVQAYHHIVSGSRRRGEEVLIAIADGGVGVRETLARNPEYAE
                     HTTTDNDALRHALEMGVSATGEIGRGGGLALVAGIAARAGGSLSLRSGSGRVTVYENR
                     KNSRNVPEFPGTFVRVSLPRTPQERSSY"
     gene            complement(3502..>3735)
                     /locus_tag="VNT12_03965"
     CDS             complement(3502..>3735)
                     /locus_tag="VNT12_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI45306.1"
                     /translation="RGRGYATEAVSLLIDYVFHTLGLAAVRGSTLAHNDRMQRVFEKN
                     GFVLTGDGSLLSRYDNRRYTELFYERREEDHKP"
BASE COUNT          633 a         1238 c         1115 g          749 t
ORIGIN      
        1 atgggctggc tgacctcgcg caggcgcctg gcctcctccg gttttacacc gagcctctcg
       61 acgagctcct cctcggtagc gtcgcggccg agctcgatgc tgagagcgtt ctcggcgcgt
      121 cgcaggcggt agagagcgtc aacgacgtgt gcgggcagac gcacggtgcg ggcgtggtcg
      181 gcgacggcgc gggtgacagc ctgacggatc caccacgtcg cgtaagtcga gaagcggttg
      241 cccatctcgg ggtcgaactt ctcgacggcc cggatcaggc cggcgtttcc ctcctggatc
      301 aaatcctcga agggcacgcc acggcccctg tacttcttgg cgatggagat gactagcctc
      361 aggttcgact cgatgagctt ctgacgggcc ctcatgtccc ccccgcggac acgcctggcg
      421 agagccttct cttcaccggc gtcgagcagc ctgccacccc gcacccttcc caggtactgt
      481 gtcagaacgt cctgtgcttc tatcattacc tctcccctct ctattagctg gtcagcattt
      541 cagctttcgg attcgcaagc ccgggatgaa gtgctgttca tcatgccgcc gggtacgatc
      601 ccccgacccg tgggcctccg tgggtcacga agcgttccag gatgcgatca tccaggctgg
      661 atgtgatgcg ccagctccgt ccgcacgagc aatctagctc gacgcgccgc cgctcgccca
      721 cttcggacat ggccatctct ttgagggagc gcaagcagca cgtaggcaac gaccacacgt
      781 agatctcgtg ctcgagctcg cctacgggga cgagcgcggt atcgggcctg agggcgctcc
      841 gcctcttgcg cgctttgcgc tgtgcttctc cgctcacctt gggtctccct ctctccccca
      901 tcgtgtgcca accttatccc tacgctgctc ctctacgctc ttcgcgctgt gcgaaggcgt
      961 cctcctcggg ttgcagaggc agcggctccc tccacatagc gccgcatacc gggcactcga
     1021 acgagtcgtc gaagtgaagg gcgctgcgcc tgcagcaggc ggggaggtcc tcctcgtaaa
     1081 cgacctcctc gccgagggca gcgatccagg cggacccacc cactacgcgg ccctccccga
     1141 aagacgcccg atccactcgc tcgcctcctc gcgggtaagc tctcctactg gcttcccgac
     1201 catctcctca aagcggccga tcccatcttc gacgacatca gagatgaggg cctcaaggta
     1261 gttgagctgc ttcctggtcg ccggcagctc gccccttcct tcctcgtgct gcgccttggg
     1321 ccgcccttca cgctcctgtg tccccgaaga ctcaacgacc tcttcctcgc cgccgagttc
     1381 ctcgaaagcg gtaacgccga cgttgatggc gtccctgaga gcgcgcgcct tggcccgcgt
     1441 ctcggccatc cggatcaggt gcggagctat cgtccggttc acgttctgcg gagaagcgtc
     1501 tccgatgccg ccgaacttgc cctcctccgt ccggatcacg gccttcacga tcgcgacctc
     1561 gccgttctcc ttcgaaggaa cctgtaaaag ctcggtctcg atgctcctga gcccccggct
     1621 gtgcgcctcc tcaagaagcc ccgcatagag cacgaacctc ttgccctggc gctcgatcat
     1681 gtactcttcc ctcatcactg aacctcctcc gaccagaagc cggtaacgct atcttcactg
     1741 aactcggtga ggatgtggtc gaaaataaac atcaggcgat ccgctcgtag gtatactggc
     1801 gtccgcctgg ggcggcggtt tcgatgcggc ggtggatcag gccgagtttc gcgagcctgg
     1861 tcgccctgtt gttgcaagct gtgtggttga gatccaacct cgctgcgagg gctgcgttgg
     1921 tgatggaacc tgcgtcgtag atcgcctgca gggtctcgac caggtggtct ggcaccttgc
     1981 caattatgcc cggctcggcc ccgatctcat cgacccgaat catggcgagc gaacgctgcc
     2041 gtagagaagc ttcgacgttc tccaggagat cacggtcctc ctcgaccagg accagatgcc
     2101 tgtccccata ctccccactg gcagacctcg acgccagtat acccaacgcc tcatccgcaa
     2161 acgagtagtc catgagctcg accccccgcg tgtccacata caggatgcca cccttcggca
     2221 attcctccag cgccccttcg agcgcctcac gaaccgcacg gcccgtcctg cgggtcacca
     2281 tcaacttgcc cccgaagtcg cccccggcga cctcgaaaac ccggtgcgcc cgctcctcaa
     2341 tcaccggatc tggcctcact gtcgtcagtg aaatcatata tgaggattgt accggtcttc
     2401 gctggataag tcaatggcat agtggggagt attttgtgtt caataggatg atctttcctg
     2461 tggggtgcgg ggcaaggaga cgcggacgaa ggtgcctgga aattcgggga cgttacggga
     2521 gttcttacga ttttcgtaga cggttacgcg gcctgagcct gagcgcaaag agagggagcc
     2581 gccggcgcgg gcggcgatcc ctgctacgag ggcgagtccg cctccgcggc cgatctcacc
     2641 ggtcgccgag acgcccatct cgagtgcgtg gcgcagggca tcgttgtcgg tggtggtatg
     2701 ttcggcgtac tcgggattac gagcgagcgt ctctcgcaca ccgacgccac cgtcggcgat
     2761 cgcgatgagg acctcttcgc cgcgacggcg ggacccactg acgatgtggt ggtaggcctg
     2821 gacggcggcg taggccccga aagaggagac gccgtgctct gcagcgttcg cgcagacctc
     2881 cgagagtgtg gagcagatcg cgacccgctc gcgcaagcgg tagccctggt tctcgaggat
     2941 ctcggagatc ctttcgatga tccctggtat ctcctcctcg gtgtggaagt tgatcaactc
     3001 ctgcagcgtc cccgggttgt gccttctgcg ctcctccaga gaggccacgt cggcgttggt
     3061 gcgtacccgg ttctcgaaag acctgaagaa gttcatccgc tccagatagg ccggcacgtc
     3121 gcgtccagcg agtgcaagat ccaccacctc gcagcgtgcg ctgaggtact ccagtagtgc
     3181 cgcaagggcg cacagcccgg ccggctcgac gaactcgacc gtgcgcaggt ctacgaccac
     3241 aggctctccg tgcacgctgg tggcggcggc gaacgcggga tcgaggctac ccatggtcag
     3301 caccccgctc atcgcgcccc ggctcctgat gttcagctcc aactgcgtct tcatcagcct
     3361 tattgtagtc ggttagggca agatagcgaa tggaaagcgg tgccggggca ttgcgggagc
     3421 cggcattttg ctcctggaga acggcgccct ggtagcgggc actctggaga cgtctgtggc
     3481 aattccgacg gagatcttac cttacggctt gtgatcctcc tcccggcgtt cgtagaagag
     3541 ttctgtgtag cgacggttat cgtagcgact cagtagggaa ccgtcaccgg ttagcacaaa
     3601 gccgttcttc tcgaagacac gttgcatgcg gtcgttgtgg gccagggtcg agcctcggac
     3661 ggctgcgagg cctagggtat ggaagacgta gtctatgagg aggctaacgg cttcagtagc
     3721 gtaacctctg cctcg
//
LOCUS       DATMJA010000168         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_672933, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000168 DATMJA010000000
VERSION     DATMJA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672933"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..785
                     /gene="purC"
                     /locus_tag="VNT12_03970"
     CDS             60..785
                     /gene="purC"
                     /locus_tag="VNT12_03970"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWI45307.1"
                     /translation="MTEELLYEGKAKKVYTTDDENMLLHRYKDEATAFNAQKRGSWKD
                     KGKTNATMSAAIFAYLETHGVPTHFVEQVDDTTIKTRKLDMLPVEIVVRNVAAGSLSR
                     LLGFEEGRKLKAPIVELCYKDDELGDPLLNWYHFRELGVSDEDLEFCEELGLKVDEVL
                     APFFDERGIVLVDFKIEAGRDAEGDLMLADEISPDTCRFWDKETGEKLDKDRFRNDMG
                     GVEEAYAEMLRRVTEITQRGAVD"
     gene            782..1006
                     /gene="purS"
                     /locus_tag="VNT12_03975"
     CDS             782..1006
                     /gene="purS"
                     /locus_tag="VNT12_03975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWI45308.1"
                     /translation="MKIRVLVRPKAGILDPQGEAVKNALPALGFEGIRTVHVGRLVEL
                     ELESADEVDAMCRRLLSNPTIEEYEWEVVG"
     gene            1003..1665
                     /gene="purQ"
                     /locus_tag="VNT12_03980"
     CDS             1003..1665
                     /gene="purQ"
                     /locus_tag="VNT12_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWI45309.1"
                     /translation="MRIGVTVFPGSNCDRDALYGVERMGADAVELWHADADLKGVDAV
                     IVPGGFSYGDYLRPGAIARFANVMGPLEEFAKGGGPVLGVCNGFQVLTEAHLLPGALL
                     RNTHMRFVCDRIRVRVESVDTPWTAGCEPGEVLTLPVAHNEGNYFVHPETLADLEYEG
                     QVVLRYLENPNGSANDIAGVCNEGRNVVGIMPHPERVCDPALGSDEGLRILRSVLVGA
                     GV"
     gene            1689..>2629
                     /locus_tag="VNT12_03985"
     CDS             1689..>2629
                     /locus_tag="VNT12_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWI45310.1"
                     /translation="MGLSDFEYDRILELMGRAPNFLELSLFSVMWSEHCGYKNSRPLL
                     GRFPNEGKRVLQGPGENAGVVEVGDGWALAFKMESHNHPSAVEPYEGAATGVGGIIRD
                     ILAMGARPVALLDSLRFGPLDEERNRYVFREVVRGIGGYGNAVGVPTVGGEVEFGLAY
                     SGNPLVNAMCIGLIRVEDLVRSSATGEGNLVVLLGSKTGRDGIHGATFASDELTDESE
                     SKRSNVQVGDPFCEKMLIECCLKLLDEDLLVSLQDLGAAGITSSASEMAAKGGVGIEI
                     ETDNVPLRESGMEPTEIMISESQERMLAVVEPAKVDEV"
BASE COUNT          517 a          749 c          894 g          469 t
ORIGIN      
        1 gctgcgcaac ctcggcgcgg ttttcggaag ggttgaggag ctgaaaagga ggctgcacta
       61 tgactgaaga attactctac gagggcaagg ccaagaaggt ctatacgact gatgatgaga
      121 acatgctgct gcatcgctac aaggacgaag cgacggcatt caacgcccag aagcgcggtt
      181 cgtggaagga caagggcaag acaaacgcaa caatgagcgc ggcgatcttc gcctacctcg
      241 agacgcacgg cgtccccacc cacttcgtcg agcaggtcga cgacacgacc ataaagacca
      301 ggaagctcga catgctaccg gtcgagatag tggtccgcaa cgtcgccgcg ggctccctct
      361 cccggctgct cggcttcgag gaaggtagga aactcaaggc cccgatagta gagctttgct
      421 acaaggacga tgaactcgga gaccccctgc tgaactggta ccacttcagg gagctcggcg
      481 tctccgacga ggacctggaa ttctgcgagg agctaggcct caaggtcgac gaagtcctcg
      541 cgccgttctt cgacgagcgt ggcatagtcc tcgtcgactt caagatagag gcggggcggg
      601 acgcggaggg cgacctcatg cttgcggatg agatctcccc cgacacctgc cgtttctggg
      661 acaaagagac gggcgagaag ctggacaagg accgtttcag gaacgacatg ggaggcgtcg
      721 aagaggccta cgccgagatg cttcgaaggg tcacggagat cacacagaga ggcgcggtgg
      781 attgaagatc cgggtcctcg ttcgtccgaa ggccggtatc ctcgacccgc agggcgaggc
      841 ggtgaagaat gcacttccgg cactgggttt cgaggggatc cgaactgtgc acgtcgggag
      901 gctcgtcgag ttggagctcg aaagcgccga tgaggtcgac gctatgtgcc gcaggctact
      961 ctccaacccg acgatcgaag agtacgaatg ggaggttgtg gggtgaggat aggcgttacg
     1021 gtcttcccgg gatccaactg cgatagggac gccctttacg gtgtggagcg gatgggcgcc
     1081 gatgcggtcg agctgtggca tgccgacgcc gatctcaagg gtgttgacgc ggtgatcgtt
     1141 cccggcggct tctcctacgg cgattacctg aggcccggcg ccatcgcccg cttcgcgaac
     1201 gtcatgggcc cgctggaaga gttcgcgaaa ggcggaggcc cggttctcgg cgtctgcaac
     1261 ggcttccagg tcctgactga agctcacctc ttgccaggcg ccttgctccg caacactcac
     1321 atgcgctttg tctgtgaccg catccgcgtc cgggtcgaat ccgtggacac gccctggacc
     1381 gcgggctgtg agcctggcga agtgcttacc ctgcccgtgg cccacaacga ggggaactac
     1441 ttcgtccatc ccgagacgct tgcggacttg gagtacgaag gccaagtcgt gctgcgctac
     1501 ctcgagaacc cgaacgggtc ggcgaacgac atcgcaggcg tctgcaacga gggccgcaac
     1561 gtcgtcggca tcatgccgca tcccgagagg gtctgtgacc ccgccctcgg ctccgatgag
     1621 ggcttgagga tcctgcgttc cgtgctggtg ggggcagggg tctgaatatc caggagacat
     1681 acagctcctt ggggctctcg gacttcgagt atgaccgcat cctggagctg atgggacggg
     1741 cgccgaactt cctggagctc tcactgttct ccgtgatgtg gagcgaacac tgcgggtaca
     1801 agaactcacg cccgctcctc ggccgcttcc cgaacgaggg gaagcgggtc ttgcagggac
     1861 ctggggagaa cgccggggtg gtcgaggtgg gggacgggtg ggcgctcgcg ttcaagatgg
     1921 agagccacaa ccatccttct gcggtggaac cttacgaggg ggcggcgacg ggggtaggtg
     1981 ggatcatccg cgacatcttg gccatgggcg cgcggcccgt cgccctgctc gactcgttac
     2041 gattcgggcc gttggatgag gagcgcaacc gctacgtctt tcgagaggtc gtgcgtggga
     2101 tcggggggta cgggaacgcc gtcggggtgc ctacggtcgg aggtgaggtc gagttcgggc
     2161 tggcctactc cggtaacccg ctggtcaacg cgatgtgcat aggactgatc cgggtagagg
     2221 atctcgtccg ttcgagcgct actggcgaag gcaacctcgt cgtattgcta ggctcgaaga
     2281 ccgggcgcga cggtatacac ggggccacct tcgcctcgga cgaactcacc gacgaatccg
     2341 agtcgaagag gtccaacgtc caggtcggtg acccgttctg cgagaagatg ctgatcgaat
     2401 gttgcctgaa gctgctggat gaggacctgc tggtctcctt gcaggacctc ggcgccgctg
     2461 gcatcacctc ctcggcctcc gagatggctg caaagggagg ggtcggcatc gagatagaga
     2521 ccgataacgt gcccctgcgc gagagtggga tggagcctac cgagatcatg atctccgagt
     2581 cccaggagcg aatgctcgcc gtcgtcgagc ccgcaaaggt cgatgaggt
//
LOCUS       DATMJA010000169         3689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_80597, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000169 DATMJA010000000
VERSION     DATMJA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80597"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="VNT12_03990"
     CDS             complement(<1..114)
                     /locus_tag="VNT12_03990"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HWI45311.1"
                     /translation="MTERSTLERKRLSDLHQIASQLGIKQYRKPELAEMIYK"
     gene            complement(181..1164)
                     /locus_tag="VNT12_03995"
     CDS             complement(181..1164)
                     /locus_tag="VNT12_03995"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HWI45312.1"
                     /translation="MEYAAVTEQVALKVARLEGSGDSERAYTLATHAFREELERVPVS
                     GRVTVLREQRLDEKGRVRAPKSHPGIVSVNDSLGNRGPEADLAVEPIEGLQLLVKGQE
                     GSISAVAAAPRGSMLRTPDMYMSKLIVGPAAKGNIDIDAPVSENIEAIAKALGRRPQE
                     ITVAVLDRDRHETLIEEIRKTGARIQIRPEGDLTPAIDVGMRGADLHAIMGIGGAPEG
                     ILAAAVMKCLGGEMQARMWPTQRSQVEKLEEYGFTDPERKLTLDDLIQGTDVLFVATG
                     ITPGETLSGVRYFRGGARTHTVVMSTEPRVVRFMDTIHALEPEAGRRGFQL"
     gene            complement(1368..2684)
                     /locus_tag="VNT12_04000"
     CDS             complement(1368..2684)
                     /locus_tag="VNT12_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HWI45313.1"
                     /translation="MRALFLIDGEHYPPVVLDAMQSVQQSLDVEGVAAAFLGGTEKIR
                     EGTDYGVPLVEGRDPVSAVENALAEYEIDVVVDLSDEPVVGYKERMKIASLALYARAR
                     YLGSDFELKPPELRPVSTKPSMAVIGTGKRVGKTAISGYLARLLAREGFEPGVVSMGR
                     GGPQRPEVIEGQKMEVGSEYLLQALGRGAHAASDYYETAALSRVITVGCRRCGGGLAG
                     EPFVSNVLEGAEIANGLDTHVTLFDGSGAAMPPVQVERRVLVAGANQDPEYIVGFLGT
                     YRLLISDLVLLTMSEEPMADSEKVRGLIAAVRKVRPDLVVIPTVFRPRPVGKVEGLRV
                     GYVSTAPPAVLDTLSRHLEEHYGCEVVAASGNLSDRKRLGTDLEEMSGAEAYLTEIKA
                     AAVDVVTRRGAAEGKPVFYCDNNPVGEGLDRALLGLAREAVAINAP"
     gene            complement(2691..3515)
                     /locus_tag="VNT12_04005"
     CDS             complement(2691..3515)
                     /locus_tag="VNT12_04005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWI45314.1"
                     /translation="MGTQGRMVICQISDIHCGSPYFIPDLLERSILEINDLDPTAVVV
                     SGDLTNAGYRQEFEEAAEYVRRIRCERVMVIPGNHDSRNVGYIHFERLFGERYSILDF
                     EEAVMVAVDSSEPDLDDGRVGREHYEFIHETFAMAEDKLKIFVIHHHLIPIPGTGRER
                     NIIYDAGDVLELLADTEVDLVLSGHKHVPYSWKLENMFIVNAGTASTTRLRGNTRPCY
                     NVIEIEDGRVRVFRKYPFKDRELIVDFDSATHQYLHYEEVTGDGRPGARRQRRPLS"
BASE COUNT          634 a         1182 c         1102 g          771 t
ORIGIN      
        1 cttgtagatc atctctgcca gctccggctt gcggtactgc tttattccca gctgcgaggc
       61 gatctggtgc aggtcactca ggcgcttgcg ctccagggta gatctttcgg tcatgtttgt
      121 actccacctc tcctgtttac gatgcggaaa tgtctttcaa cggatttctt cacgggtttg
      181 tcaaagctgg aagccccgcc gccctgcctc gggctctagg gcatggatgg tgtccatgaa
      241 tcttaccaca cgaggctcgg tcgacatgac gaccgtatgc gtccttgcac cgccccggaa
      301 atacctgacc ccgctcaggg tctcgccggg ggttattcct gtcgctacga agagtacgtc
      361 ggtgccctgg atcaggtcgt ccagcgtaag cttccgctcg gggtctgtaa agccgtactc
      421 ttcgagcttc tcgacctgtg agcgttgcgt aggccacatc ctggcctgca tctccccgcc
      481 gaggcacttc atgacggcgg ctgcgaggat gccctcagga gcgcctccta ttcccatgat
      541 ggcgtgcagg tcggccccac gcatccccac gtcgatggcc ggggtgagat cgccctctgg
      601 ccttatctgg atgcgcgctc ccgtctttcg gatctcctct atcagggtct cgtgcctgtc
      661 gcggtccagg acggcgacgg tgatctcctg aggcctgcgg ccgagggcct tcgctatagc
      721 ctcaatgttc tccgagacgg gggcgtctat gtcgatgtta cccttcgctg ccgggccaac
      781 gatgagcttg ctcatgtaca tatccggggt cctcaacata ctccccctcg gggcagcagc
      841 gaccgcagag atcgaaccct cctgcccctt taccagcaat tgcaagccct cgatgggctc
      901 gaccgcgagg tcggcctcag gccctctgtt cccgagcgag tcgttcacgc tgactatccc
      961 aggatggctc tttggcgctc tgacccgccc cttctcgtcg agacgctgct cgcgcagcac
     1021 cgttactctt ccggataccg gcacacgctc cagctcctcg cggaaagcgt gtgtggcgag
     1081 cgtgtaggct cgctccgagt ctcccgaacc ttcgaggcgc gccaccttga gcgcgacctg
     1141 ctcggttacg gcggcatatt ccacggcagc cgaagctatg gttcccgagg gggaatcagt
     1201 agcgttaatg ggctctcacc gaacctccca tgacccatca agatagcccg cacgccacgg
     1261 agcactctcg tggcagggcc tgcgtcgaaa cggtgcggag tggttccaaa gaaccagcgt
     1321 acctgacaat ccaattatac actagcgccg gtaagtcgac caccggctca aggcgcgttg
     1381 attgccaccg cctcccgcgc gagtccgagg agcgcccgat ctaggccctc gcccacgggg
     1441 ttgttgtcac agtagaagac gggcttcccc tccgcagcac cccggcgcgt aacgacgtcc
     1501 accgccgcag cctttatctc ggtgagatac gcttcggccc ctgacatctc ttcgaggtct
     1561 gtgccgagac gctttctgtc ggagagattc cccgaggcgg cgaccacttc gcaaccgtag
     1621 tgttcctcga gatgcctgga gagggtgtcc agcacggcgg gcggcgccgt cgagacgtag
     1681 ccaacccgca acccctcgac ctttccgacc ggacggggcc tgaagaccgt ggggatcacg
     1741 accagatcgg gacgcacttt gcggactgcc gcgatgaggc cgcggacctt ctcactgtct
     1801 gccattggct cctcgctcat ggtgaggagg accagatctg agataagcag gcggtaggtg
     1861 ccgagaaacc ctactatata ttcggggtcc tggttcgccc ctgcgacgag cacgcgcctc
     1921 tccacctgga ccgggggcat cgccgccccc gagccgtcga agagggtaac gtgggtgtcg
     1981 aggccgttgg cgatctcagc cccctccagg acgttggaga cgaagggttc ccctgcgaga
     2041 ccgccgccgc acctgcggca tccgacggtg atgaccctgc tcagggccgc cgtctcgtag
     2101 tagtcgctcg cggcgtgcgc tccgcgcccg agggcttgga gcaggtactc gcttcccact
     2161 tccatctttt ggccctcgat gacctcgggg cgctgaggcc ctccgcggcc catggagacc
     2221 acgccgggtt cgaagccttc acgagccaag agacgcgcca ggtagcccga gatcgccgtc
     2281 ttccccaccc tcttgcctgt cccgatcacg gccattgaag gcttggtcga gacgggacga
     2341 agttccggcg gtttcagctc gaagtcgctt ccgaggtagc gtgcccgtgc gtagagggcg
     2401 agcgaggcga tcttcatccg ctctttgtag ccgacaacgg gctcgtcgga gaggtcgacg
     2461 acaacatcga tctcgtattc ggcgagcgca ttctcgaccg cggagacggg gtctctgccc
     2521 tcgaccagcg ggacgccgta gtcggtgccc tccctgatct tctcggtgcc gcccagaaag
     2581 gctgccgcaa cgccttcgac gtccagggac tgctgcacgc tctgcatcgc gtcgaggacg
     2641 acgggaggat agtgctcgcc gtcgataagg aacagcgccc gcacgaggtg ctagctcagc
     2701 ggtcgccgct ggcgacgggc ccctggtctg ccatctcccg tgacctcttc gtaatgcagg
     2761 tactggtggg ttgcggagtc gaagtccacg atcagctcgc ggtccttgaa aggatacttg
     2821 cggaagaccc tcacccgtcc gtcctcgatc tctatgacgt tgtagcaggg gcgcgtgtta
     2881 cccctgaggc gggtggtgga ggccgttcct gcgttaacga tgaacatgtt ctcgagcttc
     2941 caggagtaag gcacgtgctt gtgcccagag aggacgaggt cgacctctgt atcggcgagt
     3001 agctcgagca cgtcccccgc atcgtagatg atgttgcgct ccctacccgt tccagggatg
     3061 gggataagat ggtggtggat gacgaagatc ttgagcttgt cttcggccat ggcgaatgtt
     3121 tcgtggatga actcgtagtg ctcgcggccc acccgcccgt cgtcgaggtc gggctcggat
     3181 gagtccacgg cgaccataac ggcttcttcg aagtccagga tcgagtagcg ctctccgaag
     3241 agccgctcga agtgtatgta accgacgttc cgcgaatcgt ggttgcctgg tatcaccatg
     3301 acccgctcgc acctgatgcg cctgacgtat tcagccgcct cctcgaactc ctggcggtag
     3361 cccgcgttgg tcaggtcacc agacacaacg acggcggtag gatccaggtc gtttatctcc
     3421 aggatcgaac gttcgagcaa gtctggtatg aagtacggac tgccgcagtg gatgtcgctg
     3481 atctgacaga taaccatcct gccctgcgtg cccattcgtt ccccgattct attcaataga
     3541 gacgccgccg gaagtatagc aaagcgtgtt ttgaagcatc aggtaaacag cacgctccag
     3601 ccttcggctt ccgctttcag cacttcagct tggcaggtgg gttataggtt tctggctaca
     3661 ggtttcagga tttgttttga tcgttccca
//
LOCUS       DATMJA010000170        12010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_673352, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000170 DATMJA010000000
VERSION     DATMJA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673352"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VNT12_04010"
     CDS             <1..684
                     /locus_tag="VNT12_04010"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HWI45315.1"
                     /translation="VGNGDLGAALLHQWPEYLAFAVSFFYVGVIWLNHHALFASIRKV
                     DFGLNWINLGILGTSALVPFPTGVLASALSEGSRADQQAAVVLYAVVAALMSAAWLPV
                     FPYLHRHPDLLVDPSEAGLFRAQRSRPWVGVISFGVAAILGYLVSPVLAIPLFAWMMA
                     YHAATSEGRQANRIARLFATRRQGHTAGPAGKRMNDRPEPRDRDEPRTEGATMPNDES
                     LQDRGTTQG"
     gene            complement(750..1001)
                     /locus_tag="VNT12_04015"
     CDS             complement(750..1001)
                     /locus_tag="VNT12_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45316.1"
                     /translation="MANLYVDEDSRQLRFLDVLTSEFLGLERKHHLLPVEAVSEEDSG
                     SITLGVDQEAVESAPRFPDPHVGPDEEYQRTIREHYGSV"
     gene            complement(1011..2372)
                     /locus_tag="VNT12_04020"
     CDS             complement(1011..2372)
                     /locus_tag="VNT12_04020"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MCT family MFS transporter"
                     /protein_id="HWI45317.1"
                     /translation="MPCRAFSNHFTQRVAAERNVPFVNEHHRRYSCAGSRVYLECMSL
                     QSGRHEPSHKPASGRLARDSVRAWLVVVAAFWALFTVFGVVFSYGAFLGPIAAEFGTG
                     RAGASVVFSLTSLLFFALGAVSGPLADRFGPRPLLLAGAAALGIGLAAASHADRLWVV
                     YVTYGAGVGIGVGCVYVPVVAAVGGWFECRRTLAVGVTVSGIGLGTLVAAPLAARLIG
                     AYGWRNTYLGFAIVGSAVLVVCVLLVAAPPGSAAGGRRGTGAAIRTLTYGWLYLSNFL
                     FSIALFVPFVHLPASAEEAGVGTVAAAGLVGIIGAASIVGRIALGAVADRAGLLRGYR
                     ICFLLVGVSFALWWFGDGFWPLATFAVVLGVGYGGFVALGPVVLADLFGVDRLGGLLG
                     VLYTAVGAGSALGPPAAGLAVDLTGSYTPAIVASALLGLAAYAALLPLDARAGERPDT
                     NHD"
     gene            2704..3072
                     /locus_tag="VNT12_04025"
     CDS             2704..3072
                     /locus_tag="VNT12_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45318.1"
                     /translation="MAAQISDPYAAAALIENRGELRTNVGGTLAQTDLSPLRRTTEGE
                     DLKRRCLAEQERIRRLEEERAAILERGDPVPRLLLANLQESRIHAEELRMWGHRTFVR
                     EIRALMAECAGGYVQNEHDE"
     gene            3387..4184
                     /locus_tag="VNT12_04030"
     CDS             3387..4184
                     /locus_tag="VNT12_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HWI45319.1"
                     /translation="MEQKMQIWDMTASEVRTRIREGEHLGPTSGVARGHVQANLVILP
                     SEYAFDFLKFCVRNLKPCPVLEVTDVGSPEPSVVAPGADLRVDLPKYRVYENGQLVDE
                     PTDIRSYWRDDLVSFLLGCSFTFETALLAAGLHMAHIDQGRNVPMYTTNRDCVPSRAF
                     AGPLVVSMRPFRGEEVSLAVTISARYPMMHGAPVHVGSPEALGVSDLQTPDFGDPISI
                     AEDQTPVFWACGVTPQAAAKQAKPALMITHSPGHMFVTDRLDAEYEI"
     gene            4475..5734
                     /locus_tag="VNT12_04035"
     CDS             4475..5734
                     /locus_tag="VNT12_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI45320.1"
                     /translation="MGNCVEWFDFAVYGFLATYIGANFFPSDNPTASLLATFAVFGAA
                     FFVRPLGGLVFGPIGDKLGRQRVLALVIILMSAASFAIGLLPTYATIGIWAPILLVFL
                     RLLQGFSVGGEYGGAGSFVAEYSPDERRGYMVSWLMVSTLIGFLMGSVVVTALSALIS
                     EDAMSSWGWRIPFLIAGPLGIVGLYIRLRLEETPEFRALETTGEVAQAPLKEAFVENW
                     RQILQVAGIAIIHNVGFYMVFTYMPTYFSTELGFGQTASFVSIVLAGLTGLVLVPPLG
                     ALSDRVGRKPLLLTACACFALLTYPLFVLMNTGNLVAAILAHLALAVIEAIFISTSIA
                     VMTELFPTRVRYGGYSIGYNFSVAIFGGSAPFLATWLISVTGNPLSPAFYVIFAAVAT
                     LLTVLTVRETAQTDLLKTQPHLEEMAR"
     gene            5734..7713
                     /locus_tag="VNT12_04040"
     CDS             5734..7713
                     /locus_tag="VNT12_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWI45321.1"
                     /translation="MAPTRLGVDVGGTFTDLVALSEGGVTTSKVPSTPRDQSIGVMNA
                     IGASGVEAGALDALAHGMTVATNALLERRGARMALVTTEGFRDVLEIARQTRPSLYDL
                     TQDRPPTLVLRDLRFTLKERMGSEGELSPLDEAGLKDVLSAIEEAEVEAVAVCLLFAF
                     MHPEHERRVGEALREALPSVHVSLSSEVLPEFREYERFSTTAADAYLAPKLAAYLKNL
                     AGKVEDAGMPTPLIMQSAGGVVGIEDAVNNASAIVLSGPAGGVVGAAYVGTLSGYRDL
                     LTFDMGGTSTDVAPIIGGEAQTTTETVIAGVPIRVPMVDVHTVSAGGGSIAWADAGGA
                     LRVGPHSAGAEPGPAGYNKGGQEPAVTDANLFLGYLADRAELGGEVVLRRELSEEALQ
                     KLGNKVGLNALETALGIVRVADAEMVRALRVISVERGLDPREFALLAFGGAGGMHACR
                     LAEELGMRTVLVPRAGGVLSALGLAISDLRRDYVSPYLADLADVNVRELKELFEGMEG
                     TAAKDLEGPRHTRRADLRYRGQSFELTVEAEKPLEIDKLEESFHAVHERRYGYRMEDE
                     PVELVNLRLIATVPVDKPELRESPAKGEAESGRREANFDGEWQEVPILDRERMGKGSE
                     VVGPAIVEFREATCVVRPGWQGLVDGVGTLVLEKE"
     gene            7710..9254
                     /locus_tag="VNT12_04045"
     CDS             7710..9254
                     /locus_tag="VNT12_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWI45322.1"
                     /translation="MKTRERLDPVTLSVLTSALAGIAEEMGAVLIRGAYSSNIKERRD
                     CSTALFDGRGRMVAQAEHIPVHLGAMPEAVAAIMRHDPSPGDVFAINDPYSGGTHLPD
                     ITLVSPVAPKGEIIGYAVTRAHHSDVGGMRPGSMPSDSREIYQEGIIIPPLRLVRGGE
                     YVDDILDFILANVRTPEIRRGDLRAQIAANRIAEERVGELIERRGEEIVLAAFDEVIA
                     YTERRTREAIRELPDGVYRAEDFLEGDGTVEDEDIPVRAKVRIKDDSITIDFAGTADS
                     VRGNVNCPLPVTRSACYFALRVLLPKDMPANAGTYAPLEIRAPEGSLVNAKSPSAVVA
                     GNVETSNRIADAVLAALSEAASLPAQGQGTMNNTIIGGPGWTYYETIGGGQGASEKGP
                     GPSGVHVGMSNTLNTPIEAFELEYPMRVERYELLYGSGGEGKHRGGDGIVRSVRVLEP
                     TSLSLLTDRRRHPPRGANGGGPGKVGENFLNEEKLPPKVSRELKAGDVVTVRTPGGGG
                     YGRAEE"
     gene            9347..9481
                     /locus_tag="VNT12_04050"
     CDS             9347..9481
                     /locus_tag="VNT12_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45323.1"
                     /translation="MKAVVEEIYVAPKGSAAMERVEEVRTVEGRASRACGWIIPNPGE
                     "
     gene            complement(9493..9993)
                     /locus_tag="VNT12_04055"
     CDS             complement(9493..9993)
                     /locus_tag="VNT12_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWI45324.1"
                     /translation="MAGLFLVEDVATAEGPALRLVAVVLLHQTIYLYGFVRPESGQVF
                     WMIVPTVNTELFSLALGEFAKEVGVGKERHILLILDKAGWHTGGEVDLPEGIHLEFLP
                     PGSPELMPAERLWPLTNEALANGLFEEIEEVEEALVQRCVELLDQPEAIRELTNYHWW
                     PQEEAA"
     gene            10284..10688
                     /locus_tag="VNT12_04060"
     CDS             10284..10688
                     /locus_tag="VNT12_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWI45325.1"
                     /translation="MSVEENKALVRREQAELWNHTGDLDAAAELFAPDRVEDAKQEAA
                     NVRRGFPDLESTIEDLIAERDKVVAHWRAQATHQEEYMGIPPTGNRVDFRGISIYRIE
                     GGKIAESWGVSDLLGMMQQLGTVREPGQAQGA"
     gene            10877..11350
                     /locus_tag="VNT12_04065"
     CDS             10877..11350
                     /locus_tag="VNT12_04065"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HWI45326.1"
                     /translation="MSGEEENQRHGEDEQQRRNREVIELLNELRVALPGVQVLFAFLL
                     IVPFSQGFSSVTPQQRYVYFATLLCTGISAALLIAPSSHHRLLWRQHAREERLKAANR
                     LSIGGLAFLALAMIGAIFLITDVLFGGVAAAVSAVGLGAVFVWYWYEQPLLSLRK"
BASE COUNT         2383 a         3773 c         3782 g         2072 t
ORIGIN      
        1 gtcggcaacg gggatctcgg tgcagcgctt ttgcaccagt ggccagagta cctcgccttc
       61 gccgtgtcgt tcttctacgt cggagtgatc tggctcaacc accacgccct gttcgccagc
      121 atccgcaagg tagacttcgg gctcaactgg atcaacctcg ggatcctggg aacctcagct
      181 ttggtcccct tcccgaccgg tgtcctggca tcggcgctga gcgagggctc gcgcgcagac
      241 cagcaggcgg cggtggtgct gtacgcggtc gtggcagcgc tgatgtcggc cgcctggttg
      301 ccggtgtttc cctatctcca ccgtcaccca gacctgctag tagacccctc cgaggcaggg
      361 ctgttccgcg cccagcgctc gcgcccctgg gtgggcgtga tcagcttcgg cgtggcagcg
      421 atcctcgggt acctcgtcag ccccgtactc gcgatccctc tcttcgcctg gatgatggcc
      481 taccacgccg cgacgagcga agggcggcag gccaaccgga tcgcacgcct gttcgcgaca
      541 agacgtcagg ggcataccgc cggacccgct ggtaagcgca tgaatgatcg cccagagccc
      601 cgcgaccgcg atgagccccg taccgaaggg gcaaccatgc ccaacgacga gtccctccag
      661 gaccgaggga ccacacaggg ttgactacct cccgcttccc tattcaccca tcctcctaga
      721 agaggaattc tccgaagttc gggcttctgc tacacggagc cgtaatgttc gcggatggta
      781 cgttggtact cctcgtcggg cccaacatgc gggtccggga agcgcggcgc gctctccaca
      841 gcctcttggt ccacccctag tgtgatcgag cctgaatctt cttcgctcac ggcttccaca
      901 gggagcagat ggtgctttct ttccaagccc aggaactcgc tcgttaagac gtccaagaag
      961 cgtagctgcc tgctgtcctc atcgacgtag aggttcgcca ccgtccctca tcagtcgtgg
     1021 ttcgtgtccg gtctttcgcc ggcgcgggcg tcgaggggca agagtgccgc gtacgccgcg
     1081 aggccgagca gcgcggaggc gacgatcgcg ggcgtgtagc tgccggtgag gtcgaccgcg
     1141 agcccggcgg ccggcgggcc tagcgccgag ccagccccga ccgcggtgta gagcacgccc
     1201 aagaggcctc cgagcctgtc gacgccgaac agatcggcga gcacgaccgg gcccagcgcc
     1261 acgaacccgc cgtagccgac ccccagcact acggcgaacg tcgcgagcgg ccagaaaccg
     1321 tcgccgaacc accacaacgc gaagctcacg ccgacgagca agaagcagat ccggtagccg
     1381 cgcagcagcc cggcacggtc ggcgacggcg ccgagcgcga tccgcccgac gatgctggct
     1441 gcgccgataa tcccgaccag gccggccgcg gcgaccgtgc caacgcccgc ctcctcggcg
     1501 gaggcgggaa ggtgcacgaa cggcacgaac agcgcgatgc tgaacaggaa gttcgacagg
     1561 tacagccaac cgtaggtcag agtgcggatc gccgcccccg ttcccctcct tccgcccgcc
     1621 gcgctcccgg gtggcgcggc gacgagcagg acgcacacga ccagcacggc cgaccccacg
     1681 atggcgaacc caaggtaagt attccgccat ccgtaggcgc cgatgagccg cgcggccagc
     1741 ggcgcggcca ccagggtacc cagcccgatg ccggacaccg tcacgccgac cgccaacgtg
     1801 cgccggcact cgaaccagcc accgaccgcg gcgaccaccg gcacgtacac gcagccgacc
     1861 ccgatcccga ccccggcgcc gtaggtaacg tacacaaccc acagtcggtc ggcatgggag
     1921 gcggcagcca gaccgatacc gagcgcggcg gcgccggcca gaagcagcgg tcgaggcccg
     1981 aaacggtccg ccagcgggcc actgaccgca cccagcgcga aaaagaggag cgaggttagc
     2041 gagaaaacca ccgaggcccc cgcccgcccg gtcccgaact cggccgcgat agggccgaga
     2101 aacgccccgt agctgaagac aacaccaaaa accgtgaaca gagcccagaa ggcggccacg
     2161 accaccaacc aggcccgcac tgagtcgcgg gcaagacgcc cggacgcagg cttgtgcgaa
     2221 ggttcgtgtc gcccggactg cagactcatg cactcaagat ataccctaga tcctgcgcag
     2281 gaatatctcc gatgatgctc gttgacaaaa ggaacatttc gttcggcagc aacacgttgg
     2341 gtaaagtggt tagaaaaggc ccgacagggc acagccgatt tccggcagga cgaagctcaa
     2401 acgctcacgg agagcattaa aaaccggagg gcaccactac tcgccaaacc tgacgagtga
     2461 gctgtccgct acttgggatg atccgggctc aggcgaagtg cctggaactt ctttacttcg
     2521 cgaggtcctc caacatcggc tgcagcttgt acttgcgccc atgacgggcg gtaagctaca
     2581 catacgttac tcacctattg gcggagcctc taaccccgcg ggcacacgtc gccaagagta
     2641 atattaagaa acattaacat cgatggtttt ggttccgctg ctatccccat accgagtagg
     2701 aaaatggcgg ctcaaatttc agacccatac gcagcagcgg ccctgatcga gaaccgtggc
     2761 gagcttagga caaacgtggg cgggacgctg gcgcaaacag acctcagtcc ccttcggcgc
     2821 acaaccgagg gggaggacct aaagcgccgg tgtctcgcgg aacaggaacg aatccgtaga
     2881 ttagaggagg agcgcgcggc tatcttagag cgcggggatc cggtgccgcg cctcttgctc
     2941 gccaacctcc aggaatcgag gatccacgcc gaagagttgc gcatgtgggg tcatcggacg
     3001 ttcgtgcgcg agatccgtgc gctcatggcg gagtgcgcgg ggggctatgt gcaaaatgaa
     3061 catgatgaat gatgagattt tttgtttctc ccggcttcgg ggaaggggta gcgattgata
     3121 ggtgagaagg aaagcaaggg taggccccac gcgacgggga cgccgcccag gacctcgccc
     3181 gagagctggc ggacctagcc gcacagctcg cttccctgaa gggcgaggcc aacgcctggc
     3241 tctccggcga gactgcgacg cgcaagaagc cttttctaca agttaagaag gcgagacgga
     3301 agccgatgcg acctcttgtt actggctctg caacaaggaa tcacggtgta gtagagtaac
     3361 cttgcgaaaa ggcgaaaggg gaccgggtgg aacaaaagat gcagatatgg gatatgaccg
     3421 ccagcgaggt gcggactcga atccgtgagg gggaacattt aggaccaacc tccggcgtgg
     3481 cgcgggggca cgtacaggcg aacctcgtga ttctccctag cgagtacgct ttcgacttct
     3541 taaagttctg tgtgcgaaac ctgaagccgt gcccggtcct ggaggtcaca gatgtgggtt
     3601 ctccggaacc ttcggtggtg gctccgggcg cagacttgcg ggtggacctt cccaagtacc
     3661 gcgtctacga aaatggtcag ctcgtcgatg agcccacgga catacggtcg tactggcggg
     3721 atgacctggt gtcgttcctg ctggggtgca gttttacctt tgaaaccgcg ttacttgctg
     3781 caggcttgca tatggcgcac atcgaccagg gccggaacgt ccctatgtac acaaccaacc
     3841 gtgattgtgt cccatcgagg gccttcgccg gaccgctggt cgtaagcatg cgtccgttcc
     3901 gtggagaaga ggtctctctg gcggtgacca tctctgctcg atacccgatg atgcatggag
     3961 ctccggtaca cgttggaagt ccggaggctc taggcgtaag cgatctgcaa accccggact
     4021 ttggcgatcc catctcgatc gcggaagatc aaacacccgt gttctgggcc tgcggtgtga
     4081 cgccccaagc agcggccaag caggccaaac ctgccttgat gatcacccac agtcccggcc
     4141 atatgttcgt taccgatcgc ttggatgcgg agtacgaaat ctgacggatc ctccaagact
     4201 tgcaggatta tccgggcagg tcaaggttca atcggatgtt gctcttcgtc gctatcgcgg
     4261 ctttgcaagc tgaacagaga tctcagagcc acaccactgc aagatgtcta gtgagatatg
     4321 gtccgaagca gcaatagtct ctatactgat tgagcagggg aacgggagac agcatggata
     4381 gaggagagcc gtggaatcac acgagcaagc ggtagcacaa gcggtaccac aggtagaaga
     4441 gacgtcgata cggaaggcca tcatcggtgc tgggatgggc aactgcgtgg aatggttcga
     4501 tttcgccgtc tacggttttc tcgctacgta catcggtgct aatttctttc catccgacaa
     4561 cccgaccgcc agcctgctcg ccaccttcgc agtcttcggc gcggctttct tcgtgcgtcc
     4621 tttaggcggg ctcgtcttcg gacctatagg cgacaagctc gggcggcagc gcgtcctggc
     4681 cctcgtcatc atcctcatgt cggccgccag ctttgcgatc gggctgctgc cgacctacgc
     4741 caccataggg atctgggcgc cgattttgct cgtcttcctg agattgttgc aaggtttttc
     4801 cgtgggcggc gagtacggcg gcgcgggttc gtttgtcgca gagtactcac cggacgagcg
     4861 cagaggatac atggtgagtt ggctgatggt ttcgacgctc atcgggttcc ttatgggctc
     4921 ggtggtggtg acggcgctgt cggccctcat ctctgaggac gccatgagct cctggggctg
     4981 gcgcatcccc ttcttgatag cgggaccgct aggcatcgtc gggctctaca taaggctgag
     5041 gttggaggaa acccccgagt tcagagccct ggagaccacc ggcgaagtcg cccaggcccc
     5101 cttgaaggag gccttcgtgg agaactggag gcagatcctc caggtcgcgg ggatcgccat
     5161 catccataac gtgggtttct acatggtttt tacctatatg ccgacttact tctccacaga
     5221 gctcggtttc gggcagaccg cctccttcgt gtccatcgtg ctggccgggc tcaccgggct
     5281 ggtgctcgtc ccacctctgg gcgcgctctc tgatcgcgtc ggacgcaagc ccttgctact
     5341 gacggcatgc gcctgtttcg cgttgctgac ctaccccctc ttcgtgctca tgaacacggg
     5401 caatctggtg gccgccatcc tggcgcacct ggcactcgcg gtcatagagg ccatcttcat
     5461 aagcacgtcc atcgcggtga tgaccgaact cttccccacg cgcgtgcgct atggcggcta
     5521 ctccataggc tacaacttct cggtggcgat cttcggaggg agcgcgccgt tcctggcgac
     5581 ctggctgatc tccgtaaccg gtaaccccct ctcgcccgct ttctacgtga tattcgccgc
     5641 cgtggcgacg ctcctcacgg tcttgacggt cagagagaca gcccaaaccg atctcctgaa
     5701 gacgcagcca catctcgaag agatggcacg ttgatggcac caacacgcct cggggttgac
     5761 gtgggcggca cgttcaccga cctggtggcg ctctccgagg gaggagtgac cacgtcgaag
     5821 gttccttcga cgccgcgaga ccagtctata ggcgtgatga acgccatagg tgcctcgggg
     5881 gtggaggccg gtgctctgga cgccctggcc cacggcatga ccgtcgcgac caacgccctg
     5941 ctcgagcgcc gcggcgcgcg gatggcgctc gtgacgacgg agggcttccg ggatgtgctg
     6001 gagatcgcgc gccagacccg cccctccctc tacgacctca cccaggatag gccccccacc
     6061 ctggtcctgc gcgacttgcg cttcacgctc aaggagagga tgggctcgga aggcgaactc
     6121 tcaccgctcg acgaagccgg tcttaaagac gtgctctccg ccatcgaaga agcggaggtg
     6181 gaagccgtcg ccgtatgcct actcttcgcg ttcatgcatc cggagcacga aagaagggtc
     6241 ggcgaggcgc taagggaggc gctccccagc gtgcacgtct cgctctctag cgaggtgctg
     6301 cccgagttca gggagtacga gcgattctcc acgaccgccg ccgacgcgta cctggccccc
     6361 aagctcgccg cctatctaaa gaatttggcc ggcaaggtgg aggatgccgg catgccgacc
     6421 cccctgatca tgcagtccgc gggcggggtg gttgggatag aagacgcagt gaacaacgcc
     6481 tccgccatcg tcctctcggg cccggccggt ggggtcgtgg gcgcggctta cgtcggaacc
     6541 ctcagcggct accgggatct tctaactttc gatatgggcg ggacgagcac ggacgtggcg
     6601 cccatcatcg gtggcgaggc gcagacgacc acggagacgg tgatagcggg cgtgccgatc
     6661 agggttccca tggtggacgt gcacacggtg agcgccggcg gaggctccat cgcctgggcg
     6721 gacgcgggcg gggctctacg cgtcgggccc cactcggcag gcgccgagcc cggtcccgcc
     6781 ggatacaaca agggcggcca agagccggcc gtcaccgacg cgaatctgtt tctcggatac
     6841 ctcgcggatc gggcggagct cggcggcgag gtggtgctgc gccgcgaact ttctgaggag
     6901 gccctccaga agctgggcaa taaggtgggc cttaacgctc tcgagactgc tttagggatc
     6961 gtgcgggtgg ccgacgcgga gatggtgcgg gcgctacgcg tgatcagcgt cgagcgcggc
     7021 ctcgaccccc gggagttcgc cctcctggcc ttcggtgggg ccgggggcat gcacgcttgc
     7081 cggctcgccg aagaactcgg catgaggacc gtgctcgtcc cccgagcggg aggggtcctg
     7141 agcgcgctcg ggctcgcgat ctccgacctc aggcgggact acgtgagccc ttacctcgcg
     7201 gacctcgcgg acgtgaacgt cagggagctc aaggagctct tcgaaggcat ggagggcacg
     7261 gccgccaaag acctggaagg gccacgacac acccgccggg cggacctgcg ctacagggga
     7321 cagtccttcg aactcacggt cgaggccgag aagcccctgg agatagacaa gctggaggag
     7381 agcttccacg cagtacacga gcggcgatac ggctaccgga tggaggacga acccgtagag
     7441 ctggtgaacc tgcgcctcat cgccaccgta ccggtggaca aaccggaact gagagagtct
     7501 ccagcgaagg gcgaggccga gtccggacgc cgggaggcca acttcgacgg cgagtggcag
     7561 gaggtgccga tcctggaccg cgagcggatg ggcaaaggtt cggaggtcgt cggaccggcg
     7621 atagtggagt tcagggaggc gacctgcgtc gtaaggcccg gctggcaggg cctggtggac
     7681 ggcgtgggca cgctcgtatt ggagaaggaa tgaagaccag agaacgacta gatcccgtaa
     7741 ccctctcggt gctgacgagc gccttggcgg gaatagccga ggagatgggg gcggtcctga
     7801 tccggggcgc ctactcctcg aacataaaag agcggcgcga ctgctcgacg gcgctgttcg
     7861 acggtagggg caggatggtc gcccaggccg agcacatccc ggtgcacctg ggggcgatgc
     7921 ccgaggcggt ggcggcgatc atgcgccacg atcctagtcc cggtgacgtc ttcgcgataa
     7981 acgaccccta ctccgggggc acgcacctgc cggacataac gctcgtctcg ccggtggccc
     8041 ctaaaggcga gatcatcggc tacgcggtca cgagggcgca ccactccgac gtgggcggga
     8101 tgcgccccgg ctcgatgccc agcgactcgc gcgagatcta ccaggagggc atcatcatcc
     8161 cgccgctcag gctggtcagg ggcggcgagt acgtagacga cattctggac ttcatcctcg
     8221 ccaacgtgcg cacccccgag atccggcgcg gggacctccg cgcccagata gcggccaacc
     8281 gcatcgccga agagcgggtg ggcgagctca tcgaacgccg cggcgaagag atcgtgctcg
     8341 ccgccttcga cgaggtcatc gcctacaccg agaggcgcac ccgcgaggcg atccgggagc
     8401 tcccggacgg cgtctaccgc gccgaggact tcctcgaggg cgacggcacg gtagaagacg
     8461 aggacatccc tgtccgggcg aaggtgagga taaaggacga ctcgataacc atagacttcg
     8521 ccggcaccgc cgactccgtg cggggcaacg tcaactgtcc gctgccggtg acgcgctcgg
     8581 cgtgctactt cgccctgcgc gtactcttac ccaaggacat gcccgcaaac gcaggcacct
     8641 acgcgccgct ggagatacgc gccccggaag gcagcctcgt gaacgccaag agcccttcgg
     8701 ccgtcgtggc cgggaacgtc gagaccagca accgcatcgc ggacgccgtt ctcgccgcac
     8761 tctccgaggc cgcctcgctg cccgcgcagg gccaggggac gatgaacaac acgatcatcg
     8821 gtggcccggg ctggacctac tacgagacca tcggtggcgg ccagggagca agtgagaaag
     8881 gccccggtcc ctccggcgtc cacgtcggga tgagcaacac cctcaacacc ccgatagagg
     8941 ccttcgagct cgaatacccg atgcgcgtcg aacgctacga gctcctctac ggctccggcg
     9001 gagagggcaa gcaccggggc ggcgacggca tagttcgctc cgtgcgggta ctggagccca
     9061 ccagcctctc cttgctcacc gaccgccggc gccacccgcc acgcggagcg aacggcgggg
     9121 gaccggggaa agtgggcgag aacttcctca acgaggagaa gttgccgccg aaggtcagcc
     9181 gggagctcaa ggccggagac gtcgtcacgg tccgcacgcc gggcggcggc gggtacggac
     9241 gagccgaaga gtaagctccg aaccggacgg cgttctcgcg tttacgggcg tgtcgtcttg
     9301 cgggtcggcg gacctttgat ctcgttgacc aggaaggagc ctggatatga aggccgtcgt
     9361 cgaggagatc tacgtcgccc ccaaaggcag cgcggcgatg gagcgcgtcg aagaggttcg
     9421 gaccgtcgaa ggccgcgcct ctcgcgcctg tgggtggatc ataccgaatc cgggtgaatg
     9481 atactcggaa cctcatgccg cctcctcctg gggccaccag tggtagttgg tgagctctcg
     9541 gatggcttcg ggctgatcta gcagttcgac gcagcgttgc accagcgcct cctcaacctc
     9601 ctcgatctcc tcaaagagcc cgttggctag tgcctcgttc gttagcggcc acaatctctc
     9661 cgccggcatc agctctgggg agcccggggg gagaaactca aggtgtatcc cttctgggag
     9721 atccacctcc cctcccgtgt gccatccggc tttgtccaag atcaatagga tgtggcgctc
     9781 cttgcccact cccacctcct ttgcgaactc cccgagcgcc aaagagaaca actccgtgtt
     9841 gaccgtgggc acgatcatcc agaaaacctg cccgctttcg ggtctcacga agccgtagag
     9901 gtagatggtt tggtgcagaa gtactacggc tacgagtcgg agagcggggc cttctgcggt
     9961 tgctacatct tcgactaaga agaggcccgc caagcattcc gccagagcga gttagcacgc
    10021 accatcccgg cagcctacca agcagaggaa atacggatag aggcctacga ggtcctcttc
    10081 ccactgtatg aggaggaacc tcacctctcc aaacaaggtc ggcacacctg acgaaggcct
    10141 attcacccag cctcgtagaa ggtgaattct gcgaacttcg cctgttgaga gggcatgcgc
    10201 tgcgctgcgg tagctggcat catactcatc ggtgggaagg gtttgacccg ctagggtcct
    10261 aagcggatac gagaaggagg agcatgtcgg tagaagagaa caaggcccta gtgcgccggg
    10321 agcaagcaga gctctggaac cataccggtg acctcgacgc cgcagcagag ctctttgccc
    10381 ccgatcgcgt cgaggatgcc aagcaggagg cagccaatgt ccgtcgaggc ttccccgact
    10441 tagaaagcac catagaggac ctcatagccg agagggacaa ggtagtagcc cactggaggg
    10501 ctcaggcaac ccaccaagag gagtacatgg gcatccctcc gaccggcaat agggtggact
    10561 ttagaggcat ctctatatat cgcatagagg gaggcaagat cgccgagtcg tggggcgtct
    10621 ccgacctttt gggcatgatg caacagcttg gcaccgtacg cgagccgggg caggcccaag
    10681 gagcatagag cttcgcccca cttctttttg cccttattca cccgagtgcg tggaagtgga
    10741 attctccgaa gttcatatcc aagatcttgc atagaaccgc ccatataggg gcggatggat
    10801 agccccattc gcttaccgcc caccgaagac cctcaaaagg gggcaaggag caccggtgac
    10861 gtagaatgtt cgagagatga gcggtgaaga ggaaaatcag cggcacggtg aagacgaaca
    10921 gcagcgccgc aaccgcgagg tcatcgaact gctcaacgag ctgcgggtgg ctctcccagg
    10981 ggtgcaggtg ctcttcgcct tcctgcttat cgtgcccttc tctcaagggt tctcttccgt
    11041 aaccccacag cagcggtatg tgtacttcgc cacgctgctg tgcacgggga tttccgcggc
    11101 gctgctcatc gcgccttctt cccaccaccg cctcctgtgg cgccagcacg ccagggagga
    11161 gaggctcaag gcagccaacc gcctgagcat aggggggttg gccttcctcg ccctggcgat
    11221 gatcggcgcc attttcttga tcacggacgt cctgttcggg ggtgtggcgg cggcggtgag
    11281 cgctgttggg ctcggcgcgg tgttcgtgtg gtactggtac gagcagccgc tgcttagcct
    11341 acgaaagtag cttctcttct cacccttatt cacaaagtgc gtggaagggc acagcccgtc
    11401 gaaaaacccc tctacgcccc ttccccagca cccttcaagg actcaaaaaa ccccgccatt
    11461 gcagtgtttt gcccttgatg tctaagtgtt tggatgcggt tttggactac tgactggtcc
    11521 cataacgact ttttcaacag gctggtcgta aagcggaagt ccaacttccg agaacgattc
    11581 ctctccaaca agagatcggt taggatgttt acggccactt caggaaaggg accccatgta
    11641 ctcccaacgt caagaagacc tagctttgtt ctcatctcat ttggaagtga agaaagccgg
    11701 ccctctggtt tgccccatct gcagccaaac caatacgatc gtgccgaagg gcgccccaat
    11761 cggagagccc tcaggcgagc ccgtcattga gagcgaccct caattcgcct acgcagcatg
    11821 cacaaattgc ggatactcaa ttttcttcga cactgaatac ctacctaaaa ggcgaagttc
    11881 gtagaactct agcaacgcga acttcgcaga attcctattc cacgcactcc ggtgaataag
    11941 ggcaagaata aaggggcgga gtcccgtagg accccggccc catgtcagta ggtgcgccct
    12001 agagctcagc
//
LOCUS       DATMJA010000171         3748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_792518, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000171 DATMJA010000000
VERSION     DATMJA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792518"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VNT12_04070"
     CDS             <1..1058
                     /locus_tag="VNT12_04070"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWI45327.1"
                     /translation="AGQTATVQRWLESLPEGPTAHDAALLLVRAWICILSGRNEDGER
                     LLTLAESIPYEGPLPDGTASVEAGVAILRAVFGLGGVRNTMEAAQHAVALEPEQPSQR
                     AALIRFGLGSSLYLSGETSQARKLFEDVVELTRTGQPLLQMVSLSYLSIVATDEGRLE
                     EAESLARQAGTLVVRFGLQGVPQSSWAPIALGYVLAKQGEMVEAQTELERGLSSRMSL
                     PGMSPWPTLIALLALARVRSERGDRSGAREVLSEARTMLQRYPDAGMFPELLEREERR
                     LRARKPRQGQLDGELTERELDVLGLLVGELSTSQIAQNLYVSPSTIRTHVKSIYRKLG
                     VSSRKDAVLEARVKRLV"
     gene            1147..1389
                     /locus_tag="VNT12_04075"
     CDS             1147..1389
                     /locus_tag="VNT12_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45328.1"
                     /translation="MESGGKQVTHMGTRMVYQIVVRGELSERYAMAFEGMEMEIKNGQ
                     TVLTGEVKDQSHLHGILDRIGALGLKLVSVENLSET"
     gene            complement(1520..2224)
                     /locus_tag="VNT12_04080"
     CDS             complement(1520..2224)
                     /locus_tag="VNT12_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45329.1"
                     /translation="MDERLAVSAVWREALTIIRRYPFATIVPAVVLAALADAPYYFLT
                     DSGFAWEQIVTFITAAFAYYLYVAYAEEVAVEAERGADRITAGGVLHELRQAAPVVPP
                     VIVASVVAITVPTAATGLLVVPGLWLLTRWSLFAPVISRERLGPIGALRRSNWLVRNH
                     FWLVFLTSTLALILEETVIHVGAVVGLFVTASDTWGEWAGGTIAASLITPLAALATSV
                     TYQRLTTPGIFRREDH"
     gene            complement(2505..2861)
                     /locus_tag="VNT12_04085"
     CDS             complement(2505..2861)
                     /locus_tag="VNT12_04085"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HWI45330.1"
                     /translation="MNVEQGPNLSPETPKAATDHPAAAPSYQQRHERGRAARRVVPRG
                     GQAEWEPAPDRPDPIDLLEAQARDRIQELIPIRYSRMMASPFAFMRGSAMGDLPQELI
                     IFFRLRLGSNYCRARR"
     gene            complement(2916..3134)
                     /locus_tag="VNT12_04090"
     CDS             complement(2916..3134)
                     /locus_tag="VNT12_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45331.1"
                     /translation="MEQNEDRSPAIEEVLVAPPTEAWLRLQVTDLLLRRPGDIRMGRS
                     AREQSDVLQQIEAALETYGSYFYPPEHR"
     gene            complement(3150..>3748)
                     /locus_tag="VNT12_04095"
     CDS             complement(3150..>3748)
                     /locus_tag="VNT12_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWI45332.1"
                     /translation="AGYRQFLEAGVRIFEYGGPMIHAKTIVADGWLSKVGSTNLNFSS
                     LVANWEIDLVVEDTDFASQMERLFEDDVSNSREIRLEGSGRHRRVRPDGRVDTSDSGV
                     RAGVVGSGTGSGATVSRVVPTLVQKGAAPLQTHEHAIAVAASGALLGASLLGARFPRL
                     VAWPLTAVGAFLGGLGLLRAVQSRLSDREPERPKSGGS"
BASE COUNT          739 a         1195 c         1145 g          669 t
ORIGIN      
        1 ctgccggcca gacggcgacg gttcaacggt ggctcgagtc gctgcccgaa gggccgaccg
       61 cgcacgacgc agcgctcctc ttggtgaggg cgtggatatg catcctctcc ggcagaaacg
      121 aggatggcga gaggctcctg acgctcgcag agagcatccc ttacgagggt ccgcttcccg
      181 acgggaccgc atcggtcgag gcgggcgtgg ctatactacg agccgtcttc ggccttggcg
      241 gcgtccgtaa cacaatggag gcggcccagc acgccgtagc gttggagccc gagcagcctt
      301 cgcagcgggc ggcgttgata cgcttcgggc tgggatccag cctgtacctc tccggagaga
      361 cgtcgcaggc gcgaaaactc ttcgaagatg ttgttgaatt gacaaggaca ggccaaccct
      421 tgttgcagat ggttagccta tcctacctgt cgatcgttgc gacggacgag ggccgcctgg
      481 aggaagccga gtcgctagcg cgacaggccg ggacgctggt ggtcagattc gggttgcagg
      541 gagtccccca atctagctgg gcacctatcg cactcggtta cgtgctcgca aagcagggtg
      601 aaatggtcga ggcccaaacc gaactcgaaa gaggtctttc gtcccgaatg agcttgccgg
      661 gtatgagccc ctggcctacc ctcatagcgt tgctcgcgct cgcgcgggtg cgctccgaac
      721 gcggcgatcg cagcggagca cgcgaggtgc tgagcgaggc gcggactatg ctgcagaggt
      781 atcccgatgc aggcatgttc cccgagcttc tggagcgtga ggaacggagg ctccgcgcgc
      841 gcaagccgcg gcaggggcaa ctcgacgggg aactcacgga gcgcgagctc gatgtgcttg
      901 gcctcctcgt cggagagttg tctacctctc agatagccca gaacctctac gtctccccga
      961 gtaccataag gacccacgtc aagtccatct accgcaagct cggggtctca tcgcgcaagg
     1021 atgccgtgtt ggaggcccgc gtcaaaaggc tcgtctagga tccaccgcta acacatctct
     1081 cctaggggag atgtgttagc gggtgaagac agaccatcct accgtgccct aacctcggag
     1141 aacgtcgtgg aaagcggtgg caaacaggtg acccacatgg gcacgaggat ggtctaccag
     1201 atagtggtgc gtggggagct cagcgagcgg tacgccatgg ccttcgaagg tatggagatg
     1261 gagatcaaga atgggcagac ggttctcacg ggggaggtga aagaccagtc acacctccac
     1321 ggaatcctcg accgcatagg cgctttgggg ctcaagctgg tgagcgttga gaacttgtcc
     1381 gaaacttgag ggggtgaatt tctccttcct gtacgcccca catcgtttaa gcacacgaac
     1441 agaagtgata gtagttagac cggaagcgaa ttcacttacc cggcaggtat ttccagaggg
     1501 caacattaga tgcaacaact caatggtcct cgcgccggaa gattccaggc gtcgtgagac
     1561 gctggtacgt aaccgaggtc gccaaagcag ccaacggtgt gatcagcgag gcagctatcg
     1621 tgccgccagc ccactcaccc caagtgtcgg acgccgtgac gaacagtcca accaccgcac
     1681 ctacatggat aaccgtctcc tccagaatga gagctagagt ggaagttagg aacaccagcc
     1741 agaagtggtt ccgcaccaac cagttgctgc gtctcagtgc acctatcggc cctagtctct
     1801 cccggctgat caccggcgcg aacaacgacc agcgcgtcag cagccacagg ccagggacca
     1861 ccaaaagacc cgtggccgcc gtggggaccg tgatggcgac caccgaggca acgatcacgg
     1921 gcgggactac cggagcggcc tgtcgaagtt cgtgtagcac gccgcctgcc gtgatgcgat
     1981 ccgcccctcg ttcggcctca accgccacct cttccgcgta tgccacgtag aggtaatagg
     2041 cgaacgctgc cgtaatgaac gtcacgatct gctcccatgc aaaacccgaa tctgtgagga
     2101 agtagtaggg agcatccgcc agcgccgcca acaccacggc ggggacgatc gtcgcgaaag
     2161 gatagcgccg aattatcgtg agtgcttcgc gccacacggc cgacacagca agccgttcgt
     2221 ccacgtacaa tccctcttcc gcgtccgccg agcagcttac tgcagagtcg cctggttcac
     2281 tcgccctcta ttttgccacg ggcgctatct acatttcttg acatgactcg cgttgccggg
     2341 ggtgaacacg cctgcgagtt cgaaacatgc ggagattcgc taaggttccc tcgcgatgtt
     2401 attcggacac cactaagaca ctacgccgta gactcaagaa ctacgaaaaa ctcggagccc
     2461 gtgaggaggg cgctgaacca cgcgcctcac gtcgcgtgcc aaaactacct ccgtgctctg
     2521 cagtagttgg agcccaggcg caaccgaaag aagattatca actcctgcgg gaggtcaccc
     2581 atggccgacc cccgcatgaa ggcgaacggc gaagccatca tgcgcgagta ccggatcggt
     2641 atgagctcct gaatccgatc cctggcctgt gcctccagaa ggtcgatcgg gtccggccga
     2701 tcgggcgccg gctcccactc cgcctggccg cccctgggga ccacccgacg cgcggcgcgc
     2761 ccgcgttcgt gacgttgctg gtaactcggg gccgccgcgg ggtgatcggt cgccgctttg
     2821 ggcgtctccg gcgagaggtt cgggccttgc tcgacattca tggcttctcc ttcacaacgc
     2881 tggaatccaa cgccactgct acagcgacca cctcgctagc ggtgctcagg ggggtagaag
     2941 tagctcccgt aggtttccag cgccgcttcg atttgttgca ggacgtcgct ctgctctcgc
     3001 gctgaccggc ccattcgtat gtcgcccggt cgcctcaaga gcaggtcggt tacctgcaac
     3061 cgtaaccacg cttcggttgg aggggccacc agcacttcct cgatcgccgg gcttcgatct
     3121 tcgttttgtt ccatatcgtc tatccttcgc tacgagccgc ccgactttgg ccgttccggc
     3181 tcgcggtcgg agagcctaga ttggaccgcg cgcagtaagc caaggccccc gaggaaagct
     3241 ccaaccgccg tcaatggcca ggcgaccagg cgcggaaaac gggcgcccag caacgacgcc
     3301 ccgagcaggg cgccgctcgc cgccaccgcg atggcgtgct cgtgcgtctg cagaggggca
     3361 gcgcccttct ggacgagagt gggcaccacc ctggagacgg tggctcccga ccccgtgccg
     3421 ctgccgacga cgcctgcgcg caccccactg tcgctggtgt ccacgcggcc gtcgggtcgc
     3481 accctccggt gtcgtcccga tccttccagg cggatctccc tgctgttgga gacgtcgtcc
     3541 tcaaagagcc gttccatctg tgaggcgaag tccgtgtctt ccaccaccag gtcgatctcc
     3601 cagtttgcga cgaggctgct gaagttcagg ttcgtcgaac ccaccttcga caaccagcca
     3661 tcggccacga tcgtcttggc gtggatcatg gggccaccgt actcgaagat gcgtaccccc
     3721 gcctcgagga actgccggta gcctgcgc
//
LOCUS       DATMJA010000172         4453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_733255, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000172 DATMJA010000000
VERSION     DATMJA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733255"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VNT12_04100"
     CDS             complement(<1..1569)
                     /locus_tag="VNT12_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HWI45333.1"
                     /translation="MSLKNRRKRDRRGRNERESTSYGHYSSSLNPFDSGKRSSQRRGL
                     AGPIIIICAIVAVLVAADFWLNSGKIHRGVEVGSVSLGGKTPAEARQIVRNQVLGPLE
                     EIDFDGPAHFTRKASEMGISFNIDQTVDKAYAVGREGNILHRLSERLRASFGGATIRP
                     DIDYHPAKARGEVREIASRVDHPPKEANVKIYGSEVEVAKSRDGYELDLAATMASVNS
                     AIDDMSGKARLRGDVLDPGVITAEAEAAAKKARGALSEQLVLKAEGKSWTISPADLGS
                     VLDVTKQDGKIDVSLNRDHLVGRLTNVYNDLTIKPVEASYDFDSDGDIFVKPSHEGRS
                     VEGEKLLDSIQGGLFDGKREYEVPVTVAKPHYTTAELEAKKPSELQGTYRTNYTATTD
                     QGQTRVENLKIASEAVSGTFVAPGETYSMLDHVANLDYYKTHVIVDGAERLDEGGGLC
                     QVTSTLYNAALYAGMEITERTPHYSQLPYIRPGMDATVWYGGPGTADDLDMKFKNTSD
                     GYVLLQEYVSNDGYI"
     gene            complement(1762..2130)
                     /locus_tag="VNT12_04105"
     CDS             complement(1762..2130)
                     /locus_tag="VNT12_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45334.1"
                     /translation="MELRNIEELRSEILQSWLRSAWVYPFQGPDERTYLRLTPGGRLK
                     IRRRIGELERALGVEGTELARQEQAGILPAERDRLELAMMVQAYDTERRLIQSQGAIL
                     GTPAVTMEEQGDEVGPDTPP"
     gene            2212..2742
                     /locus_tag="VNT12_04110"
     CDS             2212..2742
                     /locus_tag="VNT12_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45335.1"
                     /translation="MSPIHEDPPLTPWRKLGETLEGSFAAHARGLLASEFAILGREGE
                     EVGRLEIHGVEGAELGAGDLEARIERSALSGYTMLTDGAEILTAEPFGAWNTPEIKCL
                     DHLYQGWLSLLRNTARAAPAGERATVRITGGLTNRNYEAVFDAGDKGSLPVAIFLLYL
                     TVALRRKAYLTVTNRS"
     gene            complement(2861..4249)
                     /locus_tag="VNT12_04115"
     CDS             complement(2861..4249)
                     /locus_tag="VNT12_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWI45336.1"
                     /translation="MFLVGGGKRLETTVGTVLAGRYKVESTLGSGGMAVVYRAEDDIL
                     GRTVALKTLHDEYAEMPSFRRRFRQEARAMASLDHENIVKVYDISQDGEVPFIVVECV
                     SGRDLGDLLGGRRSGRLNEQFVRGMAMQLLHALSYAHRRGIIHRDIKPSNILLTSGGT
                     VKVADFGIARIVEEDDVPMDKAGEIVGSARYMSPEQLRGEDATPRSDVYSVGVLLYHC
                     LTGRPPFSGDVKSLARQHMHKDPTPPRRLNMRITPGMEAVIMKALSKKPRDRYFSANA
                     MLDDIQIEAAPKSAGMKEAPKSARRKARGGALVLASVAVLLLLLGGGGALASGLIGLP
                     QDGSVAGTLSRMNPVDTEPPAPPQGAQGESSSQNVASVTEAASSRAGTPARPKMVPVP
                     NVTAYYDYYAEDALAKRGFDVRFVYDYQDGFAPRGVTWATDPAAGTLAPRGSTVTVYA
                     TPKDLPLPRRLR"
BASE COUNT          774 a         1456 c         1321 g          902 t
ORIGIN      
        1 gatgtagccg tcgttcgaga catactcctg caagagcacg tagccgtccg aggtgttctt
       61 gaacttcata tccaggtcgt ccgctgttcc aggaccaccg taccagaccg tcgcgtccat
      121 gcccggcctg atatagggca actgcgagta atggggggtg cgctccgtta tctccatacc
      181 ggcgtagagg gccgcgttat agagcgtgga ggtaacctgg cagagtccgc ctccctcgtc
      241 cagcctctcc gcgccgtcca cgatgacgtg cgtcttgtag tagtcgaggt tggcgacgtg
      301 gtcgagcatc gagtaggtct cgcccggcgc cacgaacgta ccactaacgg cttccgaagc
      361 gattttgagg ttctctaccc gcgtctggcc ctggtccgtg gtcgccgtgt agttggtcct
      421 gtaggtgccc tgcaactcgc tcggcttctt ggcctcgagc tcggccgtgg tgtaatgcgg
      481 tttggccacg gtaacaggca cctcgtactc gcgcttgcca tcgaagaggc caccctgtat
      541 ggagtcgagt agcttctcgc cctcgacact ccgcccttcg tggctgggct tgacgaaaat
      601 gtcgccgtcc gagtcaaagt cgtagctggc ctcgacgggc ttgatcgtaa ggtcgttgta
      661 gacgttggtg agccggccga ccagatggtc gcggttgagg cttacatcga tcttaccgtc
      721 ctgctttgtg acgtcgagaa ccgaccccag atccgcgggt gagatggtcc aactcttccc
      781 ctcagctttg agcactagct gctcggagag ggctcctctg gctttcttcg ccgccgcctc
      841 tgcctcggct gttatgaccc cggggtcgag cacgtcgcct ctgaggcgag ccttcccgct
      901 catatcgtcg atggcgctgt tcacgctcgc catggtggcc gcgaggtcta actcgtagcc
      961 gtcgcgggac ttcgctacct ccacctccga gccgtagatc tttacgttag cttcctttgg
     1021 agggtgatct acccgggagg cgatctcacg cacctcgccc cgcgctttcg caggatggta
     1081 gtcgatatca ggacgtatcg tcgccccgcc gaatgaggcg cgcagacgct cggagagacg
     1141 gtggaggatg tttccttcgc gccccacggc gtaggcttta tccacagtct ggtctatgtt
     1201 gaagctaata cccatctcgc tggccttgcg ggtgaaatgc gcggggccgt cgaagtcgat
     1261 ctcctcgagc ggacctagga cctggttcct gacaatctgg cgcgcctcgg caggtgtttt
     1321 gccgcccaat gagacgctgc cgacctctac gccgcggtgt atcttgcccg agttcagcca
     1381 gaagtccgca gccacgagca cggcgactat ggcgcagatg atgattatcg gtcccgctaa
     1441 cccccgacgc tgactactcc gcttgcccga gtcgaaaggg ttcaagctgg acgagtagtg
     1501 gccgtaagac gtgctctcac gttcgtttct cccgcgcctg tcccgctttc ttcggttctt
     1561 gagactcaaa tatccaccct ccgcgagcgg ccttccactc gcccgaatga caggtttctg
     1621 gagaggtccc ccctcactct cttgactggc gacgcctcgc cctggcccga acgagcgtcg
     1681 cccggaaagc ctaacacggc aagggtgagt acgccacctc tttgccgatg gttgcaaaac
     1741 cgggcagcca ccaggggagg atcagggtgg cgtgtccggg ccgacctcgt ccccctgttc
     1801 ctccatcgtc accgcggggg ttccgaggat ggcgccctgg ctctggatca agcgccgctc
     1861 ggtgtcgtat gcctggacca tcatcgcgag ctcgagcctg tcgcgctctg cgggaaggat
     1921 gcccgcctgc tcctgcctgg caagctctgt gccctcgacg ccaagggctc tctccagctc
     1981 cccgatcctg cgccttatct tgagccgccc gcccggcgtc aggcgcaggt aggtgcgctc
     2041 gtctggtccc tggaacggat agacccatgc gctccgcaac caggactgaa ggatctcgct
     2101 cctgagctcc tctatgtttc taagctccat ttagctctct tccgtctttc gcgtaattta
     2161 cccccttcta tccggaaact ccaccgcttt cccggcgtat ggaagggtat catgagcccc
     2221 attcacgaag accccccgct cacgccctgg aggaagctcg gggagacact cgaaggctcc
     2281 ttcgcggccc acgcacgcgg actcctcgca tctgagttcg ccatcctcgg cagggagggc
     2341 gaggaggtcg gacgtctgga gattcacgga gtcgagggag ccgaactcgg agccggagac
     2401 ctggaggcgc ggatcgaacg ctccgccctc tccggctaca cgatgctaac ggacggcgct
     2461 gagattctca cggcagaacc gttcggagct tggaacacgc ccgagataaa gtgcctggac
     2521 catctctacc aaggctggct gtccctcctg cgcaacacgg cccgggccgc acccgcgggg
     2581 gaaagggcta cggtgcgcat cacaggaggt ctcaccaacc ggaactacga ggccgtcttc
     2641 gacgccggcg acaaaggctc gctacccgtc gccattttcc tcctctacct taccgtcgca
     2701 ctcagacgca aggcttatct gacggttacg aatagaagct gaaagcgcgg gctcgcccag
     2761 cggggttggt tctacagcgc cccttgaaac gattagagga caaaaagctg cactgctgac
     2821 cagcgtgctt ctaggaaggc caagccctcc gagaagcgct ttagcggagc cgcctaggta
     2881 gcgggaggtc cttgggcgtt gcgtagacgg ttaccgtgga gccccgcggg gccagcgtcc
     2941 ccgcggccgg gtccgtggcc cacgtcacgc cgcgaggtgc gaaaccgtcc tggtaatcgt
     3001 agacgaacct aacatcgaag cctcttttgg caagggcatc ctccgcgtag tagtcgtagt
     3061 aggccgtaac gttcggcaca gggaccatct tcggcctggc cggtgtcccc gcccgggagg
     3121 aagctgcctc cgtcacggag gcgacgttct ggctgctgga ctccccttgc gcaccctgtg
     3181 gcggcgctgg gggttccgta tccacagggt tcatccggct caaggtgcct gccacgctgc
     3241 catcctgtgg aaggccgatg agccccgagg ccagtgcccc tccgccgccc aggaggagca
     3301 gcagaacagc caccgaggcc aggacgagcg cgccaccacg agccttgcgg cgcgctgact
     3361 taggcgcctc cttcatcccc gccgacttgg gcgccgcttc tatctgtatg tcgtcgagca
     3421 tcgcgttggc cgagaaatac cgatccctgg gctttttgga gagggccttc atgatcaccg
     3481 cctccatgcc tggtgtgatc ctcatgttca gcctgcgcgg cggcgtcggg tctttgtgca
     3541 tgtgctgcct ggcgaggctc ttgacgtcgc ccgagaaagg cggccttccc gtcaggcagt
     3601 ggtagaggag gacacccact gagtagacgt cgctccttgg tgtcgcgtcc tctcccctga
     3661 gctgctcggg agacatgtag cgggcgctgc cgacgatctc accggccttg tccatcggga
     3721 catcgtcctc ctcgacgatg cgggcgatgc cgaaatcagc gaccttgacg gttccccccg
     3781 acgtgaggag gatattggat ggcttgatgt ccctgtgtat gataccccgc ctgtgggcat
     3841 aggagagcgc gtgcagaagc tgcatcgcca tcccccttac gaactgctca ttgaggcgcc
     3901 cgctgcggcg accaccgagg aggtccccga gatccctacc cgagacacat tcgacaacga
     3961 tgaagggcac ctcgccatcc tgggagatgt cgtatacctt gactatattc tcgtgatcca
     4021 gggaagccat cgcgcgggcc tcctgcctga agcgccgcct gaaagagggc atctcggcat
     4081 attcgtcgtg cagggtcttc agcgcgaccg tgcgaccgag gatgtcgtct tccgccctgt
     4141 agacgactgc catcccaccg gagccgagcg tgctctctac cttgtaacgt cccgcaagga
     4201 ccgttccgac cgtggtttcc agcctcttac cgcctccaac taaaaacacc tggaatgcat
     4261 caattatata tgcccgcagg cactccaggt acactaccag cgggcgagca cgacgttacc
     4321 caatatgctg gagcgtgtca aacgcgcggg ccacatctag tggcccgtcc gcgtcataat
     4381 gggccgtgat ttacccgtag acccgccgaa aggagaccat gaagaccgag ttcgacgcct
     4441 acctgcgcta cga
//
LOCUS       DATMJA010000173         2933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_733304, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000173 DATMJA010000000
VERSION     DATMJA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733304"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..946
                     /locus_tag="VNT12_04120"
     CDS             29..946
                     /locus_tag="VNT12_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI45337.1"
                     /translation="MELRQVRTFRVVAEELSFSRAAARLGYVQSSVSTQVAALERELG
                     VRLFDRLGRRISLTEAGSVMLAYSRELLVLAGEAREAVVDAGAGAGEVTGSLTVSAPE
                     TLLTYRLPRLLAIFHERHPKVRLSVRPSAIGRLVGSMRRAVDEGRVDVAFVLDGAVGV
                     PGLSIEPLIGEGVTVIAPSDHALASSTSVAPQDLSGETVLLPEAPESGCAYRGQFERQ
                     LGERGVMPQERMEFQSIEAVKQCVAAGMGVSVLISVAVEEEVGDGKLAALRWEESFEV
                     FTQMVWHEKRWKSPALRAFLETAREVFGR"
     gene            complement(970..1302)
                     /locus_tag="VNT12_04125"
     CDS             complement(970..1302)
                     /locus_tag="VNT12_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI45338.1"
                     /translation="MHDTDLIDTKELKAKLDGGDSVTLVMTLGEWEYRITHIPGSMRV
                     STVQEALEALDPEDEIVLYDSGPPCPASRMAFRILKDHGYERVRRYAGGLEAWAIAGY
                     PLEGEGVE"
     gene            complement(1423..>2933)
                     /locus_tag="VNT12_04130"
     CDS             complement(1423..>2933)
                     /locus_tag="VNT12_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI45339.1"
                     /translation="RLVHPAAWNQRLTALSWPDQPIAEQARNGAGDMLMLDELRLIPL
                     FADLSKEDLEQLYQMAETVSIPAGQRVLQEGDSGDSLYVVLDGELEVTKRQGSQDVLL
                     ALYEPGQFFGEMALLEQTPRSASVRTLRESTLLVVSQAAFQTLLSCSASAPLRILHTV
                     TSRLRSTESALIQNEKMAALGTLAAGLAHELNNPAAAIRRSAAQLSDALAERERLEAR
                     LHSLATDPHQRESLGALQEEIAERKTSTPVYDPLALSEQEDELQEWLEDHGVGEAWEL
                     APVLVSAGWDSNELERLAEPFSPAQLPVVVRWLGATSSVYGLLKEVGQSAEAMSEIVK
                     AVKTYSYLDQAPIQEVDVIESLENTLVLLRPRIDAKISITRDYADDVPRIEAYGSELN
                     QVWTNLIDNAVDAIEGQGELTLRAFATESVVTVDVIDDGPGIPQEIQPRIFEPFFTTK
                     APGVGTGLGLHIAYNIVVHKHRGRIQVASRPGETRLRVELPIRLAGNNAGVS"
BASE COUNT          495 a          868 c          969 g          601 t
ORIGIN      
        1 tcgataaagc ggaagcgaag aggtttattt ggagctcagg caggtccgga ctttcagggt
       61 ggtggcggag gagctttcgt tctcgcgggc ggcggcgagg ttgggctacg tgcagtcgag
      121 tgtgagtacg caggtggcgg cgctggagcg ggagctcggg gtgcgcttgt tcgaccggtt
      181 gggcaggagg atctcgctaa ccgaggcggg atctgtgatg ctcgcatact cccgagagtt
      241 gcttgtcctc gctggggagg cgcgggaggc ggtggtcgac gctggtgcgg gcgcgggcga
      301 agtgacgggg tcactcacgg tgagtgcccc cgagacgctc ctcacctacc ggttgccgag
      361 gcttctggcc atcttccatg agaggcatcc caaggttaga ctctcggtgc gcccgagcgc
      421 catcggccgg ttggtaggta gcatgcgccg ggcggtcgat gagggcaggg tggacgtcgc
      481 gttcgtcctc gatggggccg ttggggttcc cgggctctcg atagagcccc tgatcgggga
      541 gggtgtgacc gtcatcgccc cttccgacca cgcgctggcg tcgtcgactt cggtggctcc
      601 gcaggatctg tccggcgaga cggtgctgtt acctgaggct ccggagtcgg gttgtgccta
      661 ccggggccag ttcgagcgcc agttggggga gagaggtgtg atgccgcagg agcgaatgga
      721 gttccagagc atagaggcag taaagcagtg cgtggccgcc ggcatgggcg tctctgtcct
      781 cataagcgtg gctgtggaag aggaggtcgg ggacggcaaa ctagccgcgc tgcggtggga
      841 ggagtccttc gaggtcttca cccagatggt gtggcacgag aaacggtgga agtccccggc
      901 ccttagagcg tttctggaga cggcccgaga ggtcttcggt agatagaggc gggaacggct
      961 cgtcctctct tactctacgc cctcgccctc aagcggatag cctgcaattg cccacgcctc
     1021 cagccctccg gcgtagcgtc gtacgcgctc gtatccatgg tctttcagga tcctgaaggc
     1081 catccgactc gcggggcagg ggggaccgga gtcgtagagg actatctcat cttcggggtc
     1141 gagcgcctcg agtgcttcct ggacagtgct gacccgcatc gagccgggga tgtgcgtgat
     1201 gcgatactcc cactcgccca gggtcatgac aagggtgaca ctgtcccccc cgtcgagctt
     1261 cgctttcaac tccttggtgt cgatgagatc cgtatcgtgc attcggcaat ctcctttcag
     1321 gcaaaggctc tgcacgcagg cgttagttcc tcacgtcctc ttccatgtgg gcgggtgaac
     1381 aacagatctg tcgagaccgg caccgagtct ttcacgggtt cgctagctta caccggcatt
     1441 gtttccagcg aggcggatcg gcaactctac cctaaggcgc gtctcgccgg gtctggacgc
     1501 gacctggatc cgtccccggt gcttgtggac gacgatgttg taggcgatgt gcaggccaag
     1561 gcccgtgcct acaccgggag ccttcgtggt aaagaagggc tcgaaaatgc gcggctgaat
     1621 ctcctggggt atccccggtc cgtcgtcgat gacgtctacc gtgacaacac tttccgtggc
     1681 gaaggcgcgc agggtaagct ctccctggcc ttctatggcg tctacagcgt tgtcgatcag
     1741 gttggtccac acctgattga gctctgatcc gtaggcctcg atgcgcggca catcgtcggc
     1801 ataatcccgc gtgatgctta ttttcgcgtc gatcctggga cgcaggagca ccagggtgtt
     1861 ctccaggctc tcgatcacat cgacttcctg tatcggcgcc tgatccagat acgagtacgt
     1921 cttgacggcc ttgacgatct ccgacatggc ttcagcgctc tgtcccacct ccttgagcaa
     1981 gccgtagacc gaagaagttg ctcccagcca cctgactacg acgggcaact gcgcgggaga
     2041 gaagggttcg gccaatcgct ccaactcatt actgtcccat ccagcagaga ccagcaccgg
     2101 cgcgagctcc caggcctctc cgacgccgtg gtcctccaac cactcctgca actcatcctc
     2161 ttgctcgctg agggccagcg ggtcgtacac aggcgttgaa gtcttgcgct ccgcgatttc
     2221 ttcctgcagg gcaccgagcg attctctctg gtgcgggtcg gtggcgagcg agtggagccg
     2281 ggcctcgagc cgctctcgct cggccagcgc atcgctcagc tgtgcggcgc tacgacgaat
     2341 ggctgcggcc ggattgttca actcgtgcgc aaggcctgcg gcgagcgtgc caagggcggc
     2401 catcttttcg ttctggatca atgctgattc agtgctgcgc aacctggagg ttaccgtatg
     2461 caggatcctc aagggcgccg acgcgctgca agagagcagt gtctggaagg cggcttgact
     2521 gaccaccaac agtgtactct cccgcagtgt acgtaccgag gcagatcgtg gagtctgctc
     2581 gaggagcgcc atttcgccga agaattgccc cggctcgtac agcgcgagaa ggacgtcctg
     2641 actgccctga cgcttggtaa cctcgagctc gccgtcgagg acgacataga gcgagtcccc
     2701 agagtcgcct tcttgcaaca cgcgttgccc agcggggatg gagaccgtct cggccatctg
     2761 atacagctgc tccaggtcct ctttagagag gtcggcgaag agggggatca ggcgtaactc
     2821 gtcgagcatc agcatgtccc ctgccccgtt tctggcctgt tcagcgatcg gctggtccgg
     2881 ccagctgaga gctgtcagcc tctggttcca agccgccggg tggactaacc ggt
//
LOCUS       DATMJA010000174         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_792816, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000174 DATMJA010000000
VERSION     DATMJA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792816"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..962)
                     /locus_tag="VNT12_04135"
     CDS             complement(147..962)
                     /locus_tag="VNT12_04135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWI45340.1"
                     /translation="MELRDFQAFGEPGAIVYLADCVDLMKIIPSGSVDVVFADPPYRL
                     SRGGVTVKSGRLAPVDKGAWDRSMGFAGDHRFNVRWLKEACRILKPDGTIWVTGTHHI
                     IFSLGFALQTMRFTIINAVAWEKPDPPPNALHTAFTHAHETIVWARKGHRSRHTFNYD
                     LINSRDPSAQVSSVWRIPATPRREKRHGYHPTQKPLRLLRRAILASTHEGDLIFDPFC
                     GSGTTGVAAKELGRFFVGAEKEGEFAELAGRRIGAAVRGKVLREIPDLSAEDG"
     gene            complement(1014..2138)
                     /locus_tag="VNT12_04140"
     CDS             complement(1014..2138)
                     /locus_tag="VNT12_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWI45341.1"
                     /translation="MRASELGIRIGDLSPGPYDALTDVEGVRVGHSTIVRGEGPLVVG
                     EGPVRTGVTVVCPREGLARDEPVFAGSHRFNGNGEMTGLEWIREAGVLATPVAITNTH
                     SVGVVHDALVKAEIKARTDEEEYWCLPVVAETYDGTLNDINGMHVTAEHVFEALDSAS
                     TGPVEEGSVGGGTGMICHEFKGGIGTASRRLQEEKGGWTVGALVQANYGSRSMLRVDG
                     APVGCVLGTEIIPSPFEDEAEVQPAGTGSIIVVLATDAPLLPTQCDRLAMRASIGLGR
                     MGGGLDNGSGDIFFAFATGNRSIPRAGLSDPPATVPLQMVTNDYMTPLFHAAAEATEE
                     AILNALLAAGDMTGRDGISAHGLTPELLLGALEEVHAPGT"
BASE COUNT          419 a          749 c          718 g          411 t
ORIGIN      
        1 aagaactcct caagaaggcc ggcagactag gcgcacatag acatcgtcga gagcacgtac
       61 acctcgcttg gcaccatcgg caaggatgag cggtggctgc accagtggat caaggatgaa
      121 cgagaaggaa cggaagaaga ccgaaactag ccatcttcgg cactgaggtc ggggatctct
      181 cgcaacacct tcccacgcac cgcagcccct atcctgcgcc ccgcaagctc tgcaaactca
      241 ccctccttct ctgcccccac aaagaagcgc ccaagctcct tggctgctac cccagtcgtc
      301 ccagatccac agaatgggtc gaagatcagg tcgccctcgt gggtcgaggc aaggatggcg
      361 cggcggagga ggcgcagggg cttctgggtg ggatgatatc cgtgacgttt ctccctcctg
      421 ggcgtggcag ggatgcgcca gacggaagag acctgggctg atgggtctct gctgttgatc
      481 agatcgtaat tgaaggtatg gcgagagcgg tgtccctttc tcgcccacac gatagtctcg
      541 tgggcgtgcg tgaaggcggt gtggagagcg ttgggaggtg ggtcgggttt ctcccaagct
      601 acggcattta tgatcgtgaa tcgcatcgtc tgcaaggcga aaccgaggct gaagatgatg
      661 tggtgcgtgc ctgtgaccca gatggtgccg tcgggcttga ggatgcgaca ggcctccttc
      721 agccacctga cgttgaaccg gtgatccccc gcgaacccca tggacctgtc ccaggcgccc
      781 ttgtccacag gggcgagcct gccgctcttg accgtaacgc cgcctctgga gagcctgtag
      841 ggagggtcgg cgaagaccac gtccacgctg cccgagggta tgatcttcat gagatccaca
      901 cagtccgcga ggtacacaat ggcccccggc tcgccgaacg cctggaagtc ccttaattcc
      961 acaccaccat tttaggcagc ggcgtccgac gactttagcg atggtatagc atttcatgtg
     1021 ccaggagcgt ggacctcctc gagcgcgccg agcaggagct ccggcgtgag gccgtgggcc
     1081 gagatgccgt cccggcccgt catgtcgccg gccgccagga gtgcgttcag gatagcctcc
     1141 tccgtggcct ccgcagccgc atggaagagc ggcgtcatgt agtcgttggt caccatctgc
     1201 aaaggcacgg tcgccggggg atcggagagc ccggcgcgcg gaatgctgcg gttacccgta
     1261 gcgaaggcga agaagatgtc gccactcccg ttgtcgaggc caccccccat ccggccgagg
     1321 ccgatgctcg cgcgcatcgc cagccggtca cactgggtag gcaggagcgg cgcgtcggtg
     1381 gcgaggacca cgattataga acccgtcccc gccggctgga cctccgcctc gtcctcgaaa
     1441 ggtgaaggga tgatctcggt accaagtaca catcctacag gtgcgccgtc cacgcgcagc
     1501 atggagcggc tcccgtagtt agcctgcacg agggcgccga ccgtccagcc gcccttctct
     1561 tcctgaagcc ggcgcgaggc cgtgccgata ccgcccttga actcgtggca gatcataccc
     1621 gtccccccac cgacagagcc ctcctcaacg ggtccggtgc tagcggaatc gagcgcttcg
     1681 aatacgtgct ccgccgtgac gtgcatgccg ttgatatcgt tcagggtgcc gtcgtaggtc
     1741 tcggccacca cggggaggca ccagtactcc tcctcgtcgg tgcgggcttt gatctcggcc
     1801 tttaccaggg cgtcatgtac gacaccgacg ctgtgggtgt tggtaatcgc gacgggcgtc
     1861 gccagcacgc cggcctccct gatccattcc agccccgtca tctcgccgtt gccgttgaac
     1921 ctgtgagagc cagcgaagac gggctcatcc cgcgccagcc cctcgcgggg acagaccacc
     1981 gtaacgccgg tcctcacagg tccctctccg acgacgagcg gcccttcgcc acgcacgatg
     2041 gtcgagtgcc ctactctcac gccctcgacg tcggtgagcg catcgtaggg tcccggcgat
     2101 agatccccga tccttattcc cagctcgctg gcgcgcacgc tacctcagcg gcccagggaa
     2161 gagacgcggt ccatctcctc gtcagagagc tcgaagtcga agacgtcgag gttctccctg
     2221 aggtgctcgt cgccggtcgc cttggggatg gcacagactt tgtcctgttg cagaagccag
     2281 cgcagggccg cctgcgt
//
LOCUS       DATMJA010000175         3842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_851859, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000175 DATMJA010000000
VERSION     DATMJA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851859"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VNT12_04145"
     CDS             complement(<1..1010)
                     /locus_tag="VNT12_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI45342.1"
                     /translation="MERLGGEREGQISSIRQVALASFIGTAIEWYDFFLYGTAAALVF
                     PALFFPEFSETAGVLLAFSTYAVGFAARPLGGIVFGHYGDRIGRKAMLILSLLIMGIA
                     TFLIGLLPTYQTIGVLAPIALVVLRFCQGIGVGGEWGGAVLMAVEHAPEGRRGFFGSW
                     PQMGVPAGLLLANLVYFGSSTMLPGSWGWRVPFLLSILLVGVGLFIRLRILETPAFTQ
                     IKESGTEAPMPILDVLRTYPKNVLLAMGMRVAENGTFYVLTVFVLAYIEGELGMEQST
                     GLVGVIIAATIGLATIPLFGALSDRVGRRPVYLFGAAFSLLFAFPFFWLLNTGVAPLI
                     WIA"
     gene            complement(1222..2124)
                     /locus_tag="VNT12_04150"
     CDS             complement(1222..2124)
                     /locus_tag="VNT12_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI45343.1"
                     /translation="MKNFFLLALAGVIAQLVDGSLGMAYGVSSTTVLLAAGVAPALAS
                     AGVHMAEIGTTAVSGISHSSFGNVDWSKIVWLAVPGGVGALLGALVLVWAASLEATAA
                     YVEPAVAVFLFILGIYVLSRFAFRRTERPVKVRPVPRAFLSPLGFVAGFLDAFGGGGW
                     GPIATPTLLSSGRMEPRKIIGTVDTSEFVIAVCASIGFLLGLSLAEIPWTIVAALFVG
                     GLIAAPVAAYIVRILPTRIMGTAVGGFILVVNANTFFSAVGLSSSLSTVAFVLIIAVW
                     IAALAFAIGAKRQDQEEAAEGQAV"
     gene            complement(2284..3189)
                     /locus_tag="VNT12_04155"
     CDS             complement(2284..3189)
                     /locus_tag="VNT12_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI45344.1"
                     /translation="MANFLLLAIVGLIAQLVDGSLGMAYGVTSQSLLLTIGITPALAS
                     ASVHIAEVGTTAASGTSHWRFGNVDWSKVWPLAIPGAIGAFLGAVVLSAFITAEAAEP
                     IVAVFLFGLGVFVLARFSFRRHERPVQVRPVSKGFLAPLGLIAGFLDAAGGGGWGPIS
                     TPTLLSSGRMEPRKIIGTVDTSEFLVALSASIGFLFALTFAEIPWTVVGALLLGGVVA
                     APIAAYIVRILPARILGSAVGGLILITNMKTFLGAVGVSGALAVLIYILIVAVWIAAL
                     AFSIQVNRREKSSTVGGEASASRVV"
     gene            3454..>3842
                     /locus_tag="VNT12_04160"
     CDS             3454..>3842
                     /locus_tag="VNT12_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWI45345.1"
                     /translation="MDRPNVVVIVADTFRRDHLGAYGNPYISTPHIDEFARSSVVFDQ
                     HVISSFPTMPARADILTGTFSYTHMGWEPLPRHLTTLPGVLSKAGYHTMGVVDTPFFI
                     RNGFGYDRGFDDFVWVRGKGDDTRSHER"
BASE COUNT          849 a         1231 c         1143 g          619 t
ORIGIN      
        1 gcgatccaga tcagaggcgc aacgcccgtg ttcaacagcc agaagaacgg gaaagcgaag
       61 agcagcgaga aggcggcccc gaacaggtac accggacgac gtcctacgcg gtcggagagc
      121 gccccgaaaa gggggatggt agcgagcccg atggtggcgg cgattatgac gccgaccagg
      181 cccgtactct gctccattcc cagctcccct tctatgtagg cgagcacgaa gacggtcagg
      241 acgtagaagg tcccgttctc ggcgacgcgc atccccatcg cgagcaagac gttcttcggg
      301 taggtgcgca acacgtccag tattggcatg ggggcctcgg tgccggactc tttgatctgc
      361 gtgaaagccg gggtctccag gatcctcagc cggataaaga ggccgacacc gacgagcagg
      421 atgctgagca ggaacggcac gcgccagccc cacgacccgg ggagcatggt ggaggagccg
      481 aagtagacca ggttggcgag cagcagaccc gccgggactc ccatctgcgg ccagcttccg
      541 aagaagcctc gccggccctc cggggcgtgc tcgaccgcca tcaggacggc cccgccccac
      601 tccccgccga ctccgatgcc ctgacagaag cgcaggacga cgagcgcgat cggagccagg
      661 acgccgatgg tttggtaggt cggcaacaac cctataagga acgtcgcgat acccatgatg
      721 aggagtgaca ggatcagcat cgccttgcgt ccgattctgt ccccgtagtg cccgaagact
      781 atgccgccca gaggtctcgc cgcgaagcca accgcgtagg tcgagaacgc caggagcaca
      841 cccgcagtct cggagaactc agggaagaac agcgcgggga agaccagcgc cgccgccgtc
      901 ccgtatagga agaagtcgta ccactcgatg gcggtgccta taaagctcgc cagcgccacc
      961 tgacggatcg agctaatctg cccctcgcgt tccccaccca atctctccac accagcctcc
     1021 tttacaggcc tgagcctgat acggactccg actctccaat ccccactatg taccgaatac
     1081 tactcaaacc ctggcaccca acgcaacctg cccacacacg cctgccagca ctaccctctc
     1141 gtccgcggcg gcaacgaaaa gcggcggggc cggatcgaga tccagccccg cctattgttt
     1201 tgcgagaact cccttagaat tctagacggc ctgtccctcc gccgcctctt cctgatcctg
     1261 gcgcttggcg ccgatcgcga aggcgagcgc cgcgatccag accgctatga tgagaacgaa
     1321 ggccacggtg gagaggcttg acgagagccc gacagcactg aagaacgtgt tcgcgtttac
     1381 gacgaggata aagccgccga cggcggtacc cattatacgg gtcggcaata tgcggacgat
     1441 gtaggccgca acgggggcgg cgatcagacc gccgacgaac agagccgcaa cgatcgtcca
     1501 cggtatctcc gccaggctga ggcctaacag gaagccgatg ctcgcgcaaa cggcgatcac
     1561 gaactcgctg gtatcgacgg tgccgatgat cttgcgcggc tccatacgtc cggaggagag
     1621 cagcgtaggg gtcgcgatgg gaccccagcc tccgcctccg aaggcgtcga ggaacccggc
     1681 cacaaacccg agcggactca ggaacgcccg ggggacgggc cttaccttga cgggacgctc
     1741 ggtcctcctg aacgcgaacc ggctgaggac gtagatgcca agtatgaaga ggaatacggc
     1801 caccgccggc tcgacgtacg ccgctgtcgc ttccagcgag gctgcccaga ccagcaccaa
     1861 cgctcccaat agcgccccaa ccccaccggg gacggcgagc cagacgatct tcgaccagtc
     1921 gacgttgccg aagctcgagt gcgatattcc cgaaaccgct gtggtcccta tctccgccat
     1981 gtgtacgccc gccgaagcca gtgccggcgc tacgcctgcc gccaaaagca ccgtggtaga
     2041 cgataccccg taagccatcc ccagagaccc atccaccaac tgggcaatca caccagccag
     2101 agccaacagg aaaaagttct tcatctacaa taacctccct aaatctattt tgtctatagc
     2161 tattgtttac tttgtcagac cgctacggac tgtatagcgt gagcaacatg gtgtcaagtg
     2221 tgcggcggga gttttttgag ctcccgccgc gtaacgaaga attggagtga ttcggagggg
     2281 gtctcagacc accctgctcg cggaggcttc tccgccgaca gtgctgctct tctcgcggcg
     2341 gttgacctgg atcgagaatg caagggcggc gatccagacg gccacgatga ggatgtagat
     2401 caggacggcg agcgcgccgc tgacgccaac ggccccgagg aacgtcttca tgttcgtgat
     2461 gaggatgagg ccgccgacgg cgctaccgag gatgcgggct ggcaggatgc gcactatgta
     2521 ggcggctatc ggggcggcca cgacgccgcc gagcaggagc gcgccgacga ccgtccaagg
     2581 gatctcggcg aaggtgaggg cgaagaggaa gccgatgctc gcgctgaggg cgacgaggaa
     2641 ctcgctggta tcgacggtgc cgatgatctt acgcggctcc atccgcccgg aagagagcaa
     2701 cgtcggcgtg gagatgggac cccatccccc gccgcccgcc gcatcgagga atccggcgat
     2761 caaacccagc ggggcgagga agcccttcga gacaggcctt acctggacgg gacgctcgtg
     2821 gcgcctgaaa gagaagcggg cgagcacgaa gacgccgagc ccgaacagaa agacggcgac
     2881 gatcggctcc gcggcttcgg cagtgatgaa cgcactcaaa actaccgcac caagaaacgc
     2941 cccgatcgcc ccagggatcg cgagcggcca gaccttcgac cagtccacgt tcccgaaccg
     3001 ccagtgggag gtgcccgacg ccgcagtcgt ccctacctcc gcaatgtgca cgctcgccga
     3061 tgccagtgcc ggcgtaatgc cgatcgtcaa caataggctc tgcgaggtca ccccgtaagc
     3121 catgccgagc gacccgtcga ccaactgcgc aatcagaccc acaatcgcca acaacaggaa
     3181 attagccatc taccccaatc ctccactctt catcttgtct accatttcta ctttgtctac
     3241 catttcgatg taaaatacgt tgcgcacaat atagccccga aggggctgtg tcaaggcttt
     3301 ggacggcgaa gttgcccaaa ctcattaaaa aaatcttcga atctctcaaa ttttctacgt
     3361 aaacgctctt gacgcgccgc ccgacctgtt ctagagtccc cctcgctatt gttaatcaac
     3421 ttggtagaca aagatagcaa gaggggagag aagatggatc gtcctaacgt ggtggtgatc
     3481 gtggctgaca cgttccgcag ggatcacctt ggggcttatg ggaacccgta catcagcacg
     3541 ccgcacatag acgagttcgc gcgttcatcg gtggtctttg accagcatgt gatctcctcc
     3601 ttccccacga tgccggccag ggcagacatc ctgacgggga cgttttcgta cacgcacatg
     3661 ggttgggagc ctctgccaag gcacttgacg acgttgccgg gggtgctttc caaggccggc
     3721 taccacacca tgggtgtcgt cgacacgccc ttcttcatcc gcaatggctt cgggtacgac
     3781 cggggcttcg acgacttcgt gtgggtaagg ggcaagggcg acgacacgag atcgcacgag
     3841 cg
//
LOCUS       DATMJA010000176         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_555853, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000176 DATMJA010000000
VERSION     DATMJA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555853"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..401
                     /locus_tag="VNT12_04165"
     CDS             27..401
                     /locus_tag="VNT12_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45346.1"
                     /translation="MLGNEDPDVSVPGEILIKKAGFYDYEAKYVEGGMELVAPAPIRD
                     EITQEIRRIASTAYEAIDAAGMARVDFFLERGTDRLLLNEINTIPGFTPTSVYAELWA
                     ASGLPYEELLDRLIQLALQRHG"
     gene            404..1417
                     /locus_tag="VNT12_04170"
     CDS             404..1417
                     /locus_tag="VNT12_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWI45347.1"
                     /translation="MPFALLQETTAHLADDAKKVAKGVAEEPLSIFTGLREFLQDVYN
                     YILGPDFVGNVLASLLVAFLGVLIFRILTRGVPRILQWRRRNRESLLDEEAVARVKRQ
                     DTAITLMRNALRYVVFIIVVLVILSIFFRDPLPAAAGTTIIAAVIGFGAQSFVRDVIA
                     GFSIVFEGQYSVGDFVHIKPQDVSGIVEELGLRMTKIRSLSGEVSYIPNGTMQGVINY
                     VSGQQRFNVEVQVCDSEAATRVEHALGEASELYLSPPRLVERSEADGRVRMRIVAGVL
                     PSMAWLVEENLTQRIKAAAGEASLAAEPLIYKVDQANLRRIRSLLPQEAEKNLPRQAD
                     RKL"
     gene            complement(1550..2182)
                     /locus_tag="VNT12_04175"
     CDS             complement(1550..2182)
                     /locus_tag="VNT12_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWI45348.1"
                     /translation="MSDIPLFPLNVVLMPGAPLPLHIFEERYKQMVNECLEQEGEFGM
                     VFADESGTRKVGCTAKIVELVERYEDGRMLILVEGLRRFKLNNVLTGKPYYVGEIEYF
                     QDEPEEDVGTLAEECIALLERVVEAATEGSVGIEIEPPYRNLSFAIAGRVEFEPEIRQ
                     QILELTSEQQRLQKVKELLGAAAERLERERQTREKAQTNGHLRGDWQPGN"
     gene            2370..>3099
                     /locus_tag="VNT12_04180"
     CDS             2370..>3099
                     /locus_tag="VNT12_04180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45349.1"
                     /translation="MTEGGIHAGRVALVTGAGRGIGAATARLLAREGAAVALAARTEE
                     EIASVAHEISSHGRTALPVSVDVADEDSVSASFERVRDELGNVTILINNAGTPGIPLP
                     VAATQPEGWRRAFDVNVTGAFLCAREALPGMASANWGRIVNVSSAAARHPVAGMAAYG
                     ASKAALDQLTRVLALEGGPYGIAVTGVYPGVVDTRMQEESRGFSQELIGKQLHRMFQG
                     YRDFGMLRPPEEPAELISYLCTPAA"
BASE COUNT          583 a         1024 c          928 g          564 t
ORIGIN      
        1 tggacgcccg cgagatcgag gtctgagtgc tcggcaacga ggaccctgac gtctccgtcc
       61 ctggtgagat cctcatcaaa aaagcaggct tctacgatta tgaggccaag tacgtggagg
      121 gcgggatgga gctcgtcgcc cctgctccca tccgggacga gatcacgcag gagataaggc
      181 gcatcgccag cacggcctac gaggccatag acgcagcagg catggcgcgc gtcgacttct
      241 tcctcgaacg cggcacggac cggttgctac tcaacgagat caacaccatc cccggcttca
      301 ccccaacgag cgtatacgcg gaactctggg ctgccagcgg cctcccttac gaagaactgc
      361 tcgaccgcct catacaactg gccctccagc gccacggcta ggtatgccct tcgccctgct
      421 tcaggaaacg acggcccacc tcgccgatga tgccaagaag gtcgccaaag gcgtggcgga
      481 agaacccctg tccatcttca cggggctaag ggagtttctg caggatgtct acaattacat
      541 ccttggccct gatttcgtag gcaacgtact agcctctttg ctcgtcgcct tcctcggggt
      601 cctcatcttc cgcatcctga cccgtggcgt gccacggatc ctgcagtggc ggcggaggaa
      661 cagggagtcg ctcctggacg aggaggccgt agcccgcgtc aagcgccagg acaccgcgat
      721 caccttgatg cgcaacgcgc tccgttacgt cgtctttatc atcgtggtac tcgtgatcct
      781 gagcatcttc tttcgtgacc ctttgcctgc cgcagcgggg accaccatca tcgccgccgt
      841 tatcggtttc ggggcgcaga gcttcgtgcg cgacgtgatc gccggtttct cgatcgtctt
      901 cgaggggcag tacagtgtcg gggacttcgt ccacataaag ccgcaggatg tctccgggat
      961 cgtcgaggag ctggggctca ggatgaccaa gatacggtcc ctctcgggtg aggtgagcta
     1021 catccccaac ggcacgatgc agggcgttat caactacgtc tcgggccagc agcggttcaa
     1081 cgtggaggtg caagtctgcg actcggaggc ggccacaagg gtggagcacg ctctaggcga
     1141 ggcttcggaa ctgtacctga gcccaccacg cctcgtcgag cgaagcgagg ccgacggccg
     1201 tgtcaggatg cgtatcgtcg ccggcgtctt gccctcgatg gcttggctcg tcgaggagaa
     1261 cctgacgcag cgtataaagg ccgcagccgg ggaagcctcc ctggcggcgg aacccttgat
     1321 ctacaaggtg gaccaggcga acctgcgaag gataagatcc ctcctccccc aggaagccga
     1381 gaagaacctt ccccgccagg cggacagaaa gctctgatgg cttcaggttt caggtaggtt
     1441 acaactctcg tggcggtcta cgttttttcg tgtccccgtt cgcagaggtc tcgcacagta
     1501 caaaggcagc gcccagaaca cctgaagctc gtagcctgaa acccgattcc taattgcctg
     1561 gttgccagtc gccgcgcagg tgcccgttgg tctgggcctt ctcgcgggtt tgccgttcgc
     1621 gctcgagtct ctcggcggcc gcgcccagaa gctccttgac cttctgcagg cgttgttgct
     1681 ccgaggtgag ttccagaatc tgctggcgga tctcgggctc gaactccacc cggcctgcga
     1741 tggcgaacga caggttcctg taaggtggct ctatctcgat cccgacagaa ccttcggtcg
     1801 cagcttcgac gacccgctcg agcagggcta tacactcctc ggccagcgtg ccgacgtcct
     1861 cctcgggctc atcctgaaag tactcgatct cacccacgta gtaaggcttg cccgtaagga
     1921 cattgtttag cttgaagcgc cgtaaacctt cgaccaggat caacatccgc ccgtcctcgt
     1981 agcgctcgac gagctccacg atcttcgccg tacaacctac cttccgcgta cccgactcgt
     2041 cggcgaagac catcccgaac tcgccctcct gctccaggca ctcgttcacc atctgcttgt
     2101 agcgctcctc gaagatgtgc agcggcaaag gcgcccccgg catcagcacc acgttaagcg
     2161 ggaaaagagg tatgtcggac aagtgtgcac tcctttggct ctttgaccga attctatctt
     2221 acctggcacc agccctgctc gacacccgcg gcgacggccc ctgccgccgg tcggtgtgtg
     2281 gggtaagcta gtaggagaga cgaccgggtc cgggggcgga agatggcatc catggcgacg
     2341 agaacgtact tttctgccga caacagggaa tgaccgaagg cgggatacac gccggtcgcg
     2401 tcgcgctagt aaccggcgcg ggacgcggca tcggggcggc gacggcccgt ctcctcgcac
     2461 gcgagggcgc cgccgtcgcc ctcgccgccc gtacagagga agagatcgcc tccgtggccc
     2521 acgagatctc ctcgcacggc cgcacagccc tacccgtgag cgtggacgtg gccgacgagg
     2581 actcggtctc agccagtttc gagagggtgc gcgacgaact cggcaacgtc accatcctca
     2641 taaacaacgc gggcacaccc ggcatcccgc tccccgtcgc ggccacgcag ccggaagggt
     2701 ggcggcgcgc cttcgacgtc aacgtgaccg gggccttcct ctgcgccaga gaggcgctgc
     2761 caggcatggc ctccgcgaac tggggacgca tagtgaacgt ctccagcgcc gcagccaggc
     2821 atcctgtcgc agggatggcc gcctacggcg cctcgaaggc agccctcgac cagctcaccc
     2881 gcgttctggc cctcgaaggc ggcccttacg gcatcgccgt aacgggcgtc tatccaggtg
     2941 ttgtagacac caggatgcag gaggagtcgc gaggcttcag ccaggaactc atcggtaaac
     3001 agctccaccg catgttccag ggctacagag atttcgggat gctccgccct cccgaggagc
     3061 cggcagagct cataagctac ctctgcaccc ccgccgcgc
//
LOCUS       DATMJA010000177         4616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_674622, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000177 DATMJA010000000
VERSION     DATMJA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674622"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VNT12_04185"
     CDS             complement(<1..1193)
                     /locus_tag="VNT12_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWI45350.1"
                     /translation="MSETKQRRTYDPTDLERRWQERWAESGLYKTDEDESKPKHYALT
                     MLPYPSGDLHVGHWYPMTPSDTRARFMRMNGYRVFFPIGFDAFGLPAENAAIQRGIQP
                     REWTYANIERMRGQLRQMGAMFDFHSQVVTCAPEYYRWNQWFFLKFFEKGLAYRTEAP
                     VDWCPRDNTTLAREQVIGPDRRCERCGTPVIKKNLAQWLFKITDYAEELLDFSKIDWP
                     ERVETLQRNWIGRSEGAEIRFEIEGYGPVEVFTTRPDTLFGATFFVVSPEHPAVEKIT
                     TPEQAEEVRAYVERAARMSEIDRTDVTREKTGVFTGAYAINPANGEEIPVYIADYVLM
                     GYGTGAIMAVPGHDERDFEFARKYGIEISTVIAPPDWDGEDLVEAYTGEGTMVNSGRF
                     DGTPST"
     gene            complement(1472..1630)
                     /locus_tag="VNT12_04190"
     CDS             complement(1472..1630)
                     /locus_tag="VNT12_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45351.1"
                     /translation="MNSLRLLASPRIWGPVLGIVAVLALVNKDRGGDLAGKEVKLGDR
                     VVEECSGT"
     gene            complement(1627..2883)
                     /locus_tag="VNT12_04195"
     CDS             complement(1627..2883)
                     /locus_tag="VNT12_04195"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI45352.1"
                     /translation="MKTQKTEGLRRATCCVIGCGPAGAMLGLMLAREGVDVLVLEKHA
                     DFLRDFRGDTIHPSTMEIMDELGLAEGLLDLEHTKAPRIEARLPGGTVTVADLRRLKT
                     RYPYITFMPQWDFLDYVTREAKRYPNFRLEMKAEAKELILEDGIVRGVRYETPEGPRE
                     ARALLTVAADGRSSPFREQAGLKQVRTSPPIDVLWFRLSRRDGDPKDTFGYAGGGRFM
                     VVINRGEYWQIAYVIRKGEYQSVRADGLTAFRKSVADTIPDLADRTGELQDWEAVKLL
                     SVQADRLRRWHRPGLLCIGDAAHAMSPVGGVGINLAIADAVAAANALAGPLGAGRVRS
                     RDLAAVQLRRELPTRVIQAFQSVAQRRVVSSALGPDGAPSIPAPARALLSLRPVRDLP
                     ARIIAFGIWPEHVKRREPLARKESVK"
     gene            complement(2908..3543)
                     /locus_tag="VNT12_04200"
     CDS             complement(2908..3543)
                     /locus_tag="VNT12_04200"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI45353.1"
                     /translation="MVTKTEELGPRARAKRDQILAGARRVFLRDGFAAASTDDIAAEA
                     KVSKRTLYVYYPSKEELFAGVMRELTIENPQTRALESMEEMTPGSEEELRRDLLELAK
                     KIVVTMMQPDYLALLRTTIADTHRFPQLGGLFRATVPERAMRSFAVFIERSRECGVVG
                     PGVDGETAARMFVGPLLTYAVLDGLLAEGPPSPPAQEKIEDIVDLYMKAIT"
     gene            3829..>4616
                     /locus_tag="VNT12_04205"
     CDS             3829..>4616
                     /locus_tag="VNT12_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWI45354.1"
                     /translation="MESWQALGCLGAGRSATVIIVALLAILLAFGCGPVRPPQPPSGK
                     VIVAVGDIASCSGTADEATARLVGGIEGSTVLTLGDEAYPEGTPEEFHECYKPTWGRF
                     EDRTKPVPGNHEYHTAEAKGYFGYFGKAAGEPGKGYYSYDLGQWHIVALNSNCEEVGC
                     AATSPQLRWLEADLAKDAKTCTLAYFHYPLFSSGKYRPGVREVKPLWEALYAADADVV
                     LNGHDHNYQRFAPQDPNGKADPQRGIREFVVGTGGRSHYPIREP"
BASE COUNT          805 a         1500 c         1402 g          909 t
ORIGIN      
        1 gtgctcgggg tgccgtcgaa cctacccgag ttgaccatgg tgccttcgcc ggtgtaggcc
       61 tctacgagat cttcgccgtc ccaatccggc ggcgctatca cggtactgat ctcgatgccg
      121 tacttccttg cgaactcgaa gtctcgctcg tcgtgacctg ggacggccat gatcgcgccc
      181 gtaccgtagc ccatcaggac gtagtcggcg atgtagacgg ggatctcttc tccgttggcc
      241 gggttgatcg cgtaagcccc cgtaaatacg ccggtctttt ccctcgtcac gtcggtgcgg
      301 tcgatctcgc tcatccttgc ggcccgctcg acgtaggcgc ggacctcttc ggcctgctcc
      361 ggggtcgtta tcttctcgac cgccgggtgc tccgggctca caacgaagaa cgtggcgccg
      421 aagagcgtgt ccgggcgggt ggtgaagacc tcgactgggc cgtagccttc gatctcgaac
      481 ctgatctcgg caccctcgga gcgcccgatc cagttccgct gcagcgtctc gacccgctcg
      541 ggccagtcta tcttcgagaa gtccagcagc tcctcggcgt agtcggtgat cttgaagagc
      601 cactgcgcca ggttcttctt gataaccggc gtcccgcacc gctcgcagcg cctgtctgga
      661 cctatcacct gctcccgcgc cagcgtcgtg ttgtccctgg ggcaccagtc gacaggagct
      721 tctgtgcggt acgccagtcc cttctcgaag aacttcaaga agaaccactg gttccagcgg
      781 tagtactcag gggcgcaggt cacgacctgg gagtggaagt cgaacatcgc gcccatctgc
      841 ctgagctggc cgcgcatccg ctcgatgttc gcgtaggtcc attcgcgggg ttgtatgccc
      901 ctctggatcg cggcgttctc ggcgggcagc ccgaaggcgt cgaagcctat cgggaagaac
      961 accctgtagc cgttcatccg cataaagcgc gctctggtgt ccgagggcgt catcgggtac
     1021 cagtgcccga cgtgcagatc accactcggg taagggagca tcgtgagcgc gtagtgcttg
     1081 ggcttgctct cgtcctcgtc ggtcttgtac aggccgctct cggcccaccg ctcctgccag
     1141 cggcgttcga ggtccgtggg atcgtatgtc cttctctgtt tcgtctcgct cacgagagac
     1201 tccttcggtt aagggcttgg ggtggttcta gatcagcgga aatccgcgca gggctttata
     1261 gtcgggagtc cgacttctga atcttgtgat ccctgacgcg catacacgta atatagcatc
     1321 aggtatggcc tttacaaaag gagactgccg cccggaagtc actccgggcg gcgagtaagg
     1381 gcttgtcggc gagagttcga ccctgctttc tcagcacctt tcccggcctc tttaccttcg
     1441 cgccgggcga aaacatcacg gaacaacgag atcaggtacc cgagcactcc tcgacgacgc
     1501 ggtcaccgag cttgacctcc ttcccggcga ggtctccgcc ccggtctttg ttgaccaggg
     1561 cgaggacggc cactatgcca aggacgggtc cccagattct gggactcgcg agcaacctca
     1621 acgaattcat ttcaccgact cctttcttgc caacggctcc ctgcgcttta cgtgctcggg
     1681 ccagatgccg aacgcgatga tgcgggcagg aaggtcgcgc acgggccgca ggctcaggag
     1741 cgccctggca ggcgcgggga tcgaaggcgc tccatcaggg ccgagggctg aggatacaac
     1801 acgccgctgg gcgacggact ggaaggcctg tatcacacgc gtcggtaact cgcgccttag
     1861 ctgcaccgcc gccaggtcgc gcgaacgtac ccgtccggct cccagtggtc ctgccagggc
     1921 gttcgccgcc gcgaccgcgt ccgctatagc gaggttgatg ccgacgccgc caacaggaga
     1981 catggcgtgc gcagcatccc cgatgcacag tagcccaggc ctgtgccagc gccgcaatcg
     2041 atccgcctgc acggagagaa gctttacagc ttcccagtcc tgcagctccc ctgtacggtc
     2101 ggcgagatcc gggatcgtgt cggcgacgga cttccggaat gctgtgagac cgtcggcgcg
     2161 cacgctctga tactcacctt tcctgattac gtaggcgatc tgccagtatt cgccgcggtt
     2221 tatgacgacc atgaagcgcc cgccgccggc gtacccgaag gtatccttgg ggtcgccatc
     2281 gcgtcgcgag agcctgaacc agagcacgtc gatgggcggc gaggtcctga cctgtttcag
     2341 gccggcctgc tctctgaagg gggaagaccg tccatcggct gcaaccgtga ggagcgcccg
     2401 cgcttcgcgg ggaccttcgg gtgtctcgta gcgcacgccg cgcacgatac cgtcctccag
     2461 gatcaactcc ttcgcctcgg ctttcatctc caggcggaag ttagggtagc gcttcgcttc
     2521 ccttgtcacg tagtccagaa agtcccactg gggcatgaag gtgatgtaag ggtagcgcgt
     2581 cttcaggcgg cggaggtcag cgaccgtaac ggtccctcca gggaggcggg cttcgatcct
     2641 cggggctttg gtatgctcga ggtcgagcag cccttccgct aaaccgagct cgtccataat
     2701 ctccatggtc gaggggtgga tggtgtcccc gcggaagtcc cgcaggaaat ccgcatgctt
     2761 ctcgagcacc agcacgtcga ccccctcgcg cgccagcatg agcccgagca tcgccccggc
     2821 tgggccgcag ccgatcacac agcacgtggc acgcctgagc ccttcggtct tctgggtctt
     2881 catggcatag cctccttccc gctttcttca ggtgatcgcc ttcatgtaca gatcgacgat
     2941 gtcttcgatc ttctcttgcg caggaggact cggcggccct tcggcaagga ggccgtcgag
     3001 gacggcgtag gtgagcaagg gtcctacgaa catgcgtgcc gccgtttccc cgtcaacccc
     3061 aggaccgacg accccgcact cccggctcct ctcgataaag accgcgaagc tcctcatggc
     3121 ccgctcgggc acggtggcgc ggaagagtcc gcccaactgt gggaacctgt gggtgtcggc
     3181 aatggtagtc ctgagcagcg ccaggtagtc aggttgcatc atggtcacga cgatcttctt
     3241 tgcaagctcc aggagatccc ggcgcagctc ctcctcgctg ccaggcgtca tctcctccat
     3301 agactccagt gcacgagtct gagggttctc tatggtcagc tcgcgcatga cgccggcgaa
     3361 gagctcctcc ttgctcggat aatagacgta gagggtcctc ttggagacct tcgcctccgc
     3421 cgcgatgtcg tccgtgctgg cagcagcgaa gccatcgcgc agaaacaccc tccgcgcacc
     3481 ggccaggatc tggtcccgct tcgccctcgc cctgggtcca agctcctctg tcttcgtaac
     3541 caacgaatcc tcttatctaa gtaaactaaa cagtaccgtt tattatgaat cggctcgcga
     3601 ggtttgtcaa ggtggtctta ggttgcacac ctcgtcacga accccgggct ctgtcgtgct
     3661 tttggtgaga cggaggaaat cgacgggccg atgctctgtt tggacggctc tacgtcgtcg
     3721 tcaagactcg gacaataagc cggctctcag gaggttgtgg gtgctttcgg ctcgggcgcg
     3781 tgatcgggat catagcaatc cgctatctcc ctgggctacc ttatcctcat ggaatcctgg
     3841 caggcgctcg gatgcctcgg agccggtaga agcgcgaccg taatcatcgt ggccctcctg
     3901 gcgatactcc tggcctttgg ctgcggtccg gtacggcccc ctcaacctcc ttcagggaag
     3961 gtcatcgtcg cggtaggcga catcgccagt tgctccggca cagcagatga ggcaaccgcc
     4021 agactcgtcg gtggcatcga aggcagcacg gtgctcaccc tgggcgacga ggcctaccct
     4081 gagggcacac cggaggaatt tcacgagtgc tacaaaccca cctggggccg cttcgaggac
     4141 cgtaccaagc ctgtgcccgg caaccatgag taccacacgg cagaagccaa ggggtacttc
     4201 ggctacttcg ggaaggccgc gggcgagccg ggcaagggct actactccta cgacctaggc
     4261 cagtggcaca tagtcgccct caacagcaac tgcgaggagg tcggctgcgc agccacctcg
     4321 ccgcagctta ggtggcttga ggcagacctc gccaaggatg ccaagacctg cacgctggcc
     4381 tacttccact acccgctgtt tagctccggg aagtatcgtc ccggcgttcg cgaggtgaag
     4441 ccgctttggg aagccctcta cgctgccgac gcggacgtgg tgctaaacgg ccacgaccac
     4501 aactaccagc gtttcgcgcc gcaggaccct aatgggaagg cagaccccca gcgcggcatc
     4561 agggagttcg tggtgggcac cgggggcagg agccactacc cgatccggga gcccat
//
LOCUS       DATMJA010000178         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_260087, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000178 DATMJA010000000
VERSION     DATMJA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260087"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1385)
                     /locus_tag="VNT12_04210"
     CDS             complement(423..1385)
                     /locus_tag="VNT12_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWI45355.1"
                     /translation="MRASSPHRTGIIEVGKRSIRKFFEHPISTYAAALAYRGLFGLFP
                     FVLILVVLASTLGLADFLKGAIDQASSESAQHVPDQLEPVIERGKEQLQPLQRMIEQA
                     EEQAGGELLFLGVALALWSISALAGTLTEAFNAVYEVPETRRWWKVSALSLAFGPILA
                     LMVIVSVGLMLIGPQLVERIAALVGLDEVFVSLWEWLRFPVALTLLAVVLSLVYRFGP
                     DARQRFRSVVSGAVLAVVLWAISSVGFSFYLANFADYGVTYGSLGAAVGLLFYLYLSA
                     SVVLLGAEVNAAIYHSGPDGSEQAEKPGSDHETTDTQSKRVNDV"
     gene            complement(1665..>2553)
                     /locus_tag="VNT12_04215"
     CDS             complement(1665..>2553)
                     /locus_tag="VNT12_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWI45356.1"
                     /translation="EMVEKLPDEERPDPKDIQAAGETPARLVVQEPRRMRTLRMLEFL
                     TAGVQERLWITDPYFLSMPILTQSLMATARDGVDVRVLTPATNDIAWIGRASRAGYRQ
                     FLEAGVRIFEYGGPMIHAKTMVADGWWSKVGSTNLNVSSLAANWEIDLVVEETDFADQ
                     MEALFEDDISNSREVRLEGSGPHRRVRPDRQVDTTDSGARAGVVGGGTGSGATVARVG
                     NTLVQKGAAPRQTHEHALAAAASGALLGASLLGARFPRLVAWPLTAVGALLGGLGLVR
                     AARSTLSERGPDRPESGAT"
BASE COUNT          487 a          840 c          757 g          469 t
ORIGIN      
        1 ttctgttccc tgcattgccc accattgcag ggtattgcct ccgggttagg gtcaaactag
       61 ggtcaggtgc gagtgggcgt agtgacaagc tgcccgcaac cgagcgaatt cacgcccggc
      121 agtttacgct acctgcacta cgctggcaga gcccatattc atactacgtg gagtcgaggg
      181 aattcgaagc cctcacctcc gccatgcaaa gtcggtacag gtgctgttgc ctagcgccgc
      241 tgacgatcac tgaatcacat aaggagccga tacttaccat ccactcctca ctgcgaaatc
      301 gtctgctcgt cttcatcgtg ctcatcctcg cctgacgtgg aggcctgaaa cagccgagcg
      361 ccgaaagtat ccagggtcaa cacaacttcg aacgtggctt tcgattccgg caagctgttg
      421 cttcatacat cattcaccct cttgctctga gtgtctgtgg tctcatggtc ggaacccggc
      481 ttttctgcct gttcgctacc gtccgggccc gaatggtaga tagccgcgtt gacctccgct
      541 cccagcaaca ccacggatgc ggagagatac aggtagaaga gcagacccac cgccgctccc
      601 agactcccgt aggtgacgcc gtagtctgca aagtttgcga ggtagaagga gaaaccaacc
      661 gacgatatgg cccacagaac cacagccagc accgcccccg agaccactga gcgaaagcgc
      721 tgcctggcgt ctggaccgaa acggtagacg agcgagagca ccacggcgag cagagtgagt
      781 gctacgggga agcgcagcca ctcccacaaa gagacgaaga cttcgtcgag gcccaccaac
      841 gccgctatgc gctctaccaa ctgcggcccg atcaacatca gacctaccga gacgatcacc
      901 atgagcgcga ggatgggacc gaaggccaga gagagcgccg acaccttcca ccagcgacgg
      961 gtctcgggga cctcgtagac ggcgttgaat gcctcggtca aggtacctgc tagcgccgag
     1021 atcgaccaca gcgccagggc taccccgagg aagagcaatt ctcccccggc ttgttcttca
     1081 gcctgctcga tcatcctctg tagaggttgg agctgctcct tgccccgctc gatcaccggt
     1141 tcgagttgat cgggaacgtg ctgggctgat tcggagctag cctggtctat agcgcccttg
     1201 aggaagtctg ccaagccgag cgtgctcgcc agcacgacga ggatgagcac gaaagggaag
     1261 agaccgaaga gcccacggta ggccagcgcg gcggcgtagg tcgagatggg gtgctcgaag
     1321 aacttacgga tcgagcgttt gccgacttcg atgatcccgg tcctgtgtgg gctcgaagcg
     1381 cgcataccat cctatcggga ggataaactg caacattgct ccatttcgga gactttagcc
     1441 agggaccgcc actcggcgtt catcacgtag gctcgacctc cgcatcaggg gcaggggtgc
     1501 cggtgtccgc accactcacg cctatgagac ccgccagcca cctcgtctcg gggaaggtgc
     1561 cgattttggt tacgctcctt cgacgcttcg gcaatggagg ccaccaaccc tccctcgatt
     1621 gccgggatcc gattttcgtt ttctttcaca tcgtccatcc ttcgctacgt cgctcccgac
     1681 tccgggcggt ccggcccgcg ctcggagagc gtcgatcggg ccgcgcgtac gaggccaagg
     1741 ccaccgagta acgcgcccac tgccgtcaat ggccaggcga cgaggcgcgg aaaacgggcg
     1801 ccgagcaacg acgccccgag cagggcgccg ctcgccgcgg cggcgagcgc gtgctcgtgc
     1861 gtttgtcgag gggcagcgcc cttctggacg agggtgttac ctaccctcgc gacggtggcc
     1921 cctgatcccg tgccaccacc gacgacaccg gcgcgcgccc cgctgtcggt cgtgtccacc
     1981 tgccggtcgg gccgtaccct tcggtgtggc cctgaccctt ccaggcggac ctcccggctg
     2041 ttggagatgt cgtcctcgaa cagcgcttcc atctggtcgg cgaagtccgt ctcttccacc
     2101 accagatcga tctcccagtt ggcggcgagg ctgctgacgt tcaggttcgt agatcccacc
     2161 ttcgaccacc agccgtcagc caccatcgtc ttggcgtgga tcatcgggcc gccgtattcg
     2221 aagatcctga cccctgcctc aaggaactgc cggtagccgg cgcgcgaggc cctgcctatc
     2281 caggctatgt cgttggtggc cggcgtgagg accctcacgt ccacaccgtc gcgcgccgtc
     2341 gccatcaggg actgggtgag gatcggcatg gagaggaagt aggggtcggt gatccagagc
     2401 cgctcttgga ccccggccgt gaggaactcc agcatacgca gggtacgcat cctgcgcggt
     2461 tcctgcacga ccagacgcgc gggcgtctct cccgcagcct ggatatcctt cggatccggg
     2521 cgctcctcgt cgggcaattt ctccaccatc tcg
//
LOCUS       DATMJA010000179        11243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_319617, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000179 DATMJA010000000
VERSION     DATMJA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319617"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VNT12_04220"
     CDS             <1..356
                     /locus_tag="VNT12_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HWI45357.1"
                     /translation="NHIPPGEPILFLEVDVEDASEFEDVLEVRGVEIGGHKVLRAKSS
                     VVPNAIAALLLHLRDTTGKTPHCYFGWAEGNPIAYLFRFVLFGEGDTAPVTHEVLREA
                     EPERKRRPAIHVGGR"
     gene            complement(813..1472)
                     /locus_tag="VNT12_04225"
     CDS             complement(813..1472)
                     /locus_tag="VNT12_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45358.1"
                     /translation="MNDWTARDRRVAIWSALGTCFFGLLYTPTLVAAFVSAGNVTDPL
                     TDPFLGILEVQIILMAPFMVLTMVAVHGYAPRGTKMYSLSALGFMILCAGLTTCVHFV
                     ELTVARRIDPTSMPAYFRLFSWEWPSVDYALDIVAWDWFFGLSMLLAAPVFEGGKLHT
                     SVRACMILSGTLSIAGLLGAALGDMQLRGIGIAGYAGVFPVVCLLLALIFSRTSGTNR
                     N"
     gene            1661..2323
                     /locus_tag="VNT12_04230"
     CDS             1661..2323
                     /locus_tag="VNT12_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWI45359.1"
                     /translation="MDRILSLIEHYGYLVILLGVMLESTGVPLPGETILLASGVLVQR
                     RHRDLGDAIVFGILGSVLGDQIGYWAGREGGRPFVLRWGRYVFITPERLARAEAFFER
                     HGGKAVFLARFISGLRVFGALVAGISRMRWGTFILYNALGGVVWATAVVLVGYFLGSS
                     IGLVERWLGGATLLLLAVLAVVVVFYLAYRWAAHNRSRLVGWGDALLAYPPVDRLRTR
                     RA"
     gene            complement(2552..3184)
                     /locus_tag="VNT12_04235"
     CDS             complement(2552..3184)
                     /locus_tag="VNT12_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45360.1"
                     /translation="MDEFPYLLEQDPSLEATLQKVWDRYLREAPVLLVLIGSDISMMS
                     ALTHYDRPLYGRPTLEMVVEPFGPHETAEMLDLPAADAIDAQLVLGGFPQITQEWRAG
                     TGMWDFLEGQISNPTSPLIVGGERILSAEFPEEARARQRCSRPSAPEREPSPRYSVAR
                     ASTRRPCRGRLRCWSGRSAWCRPSARSRAGLRGRRTTRSQTPTFASGYAS"
     gene            complement(4063..4491)
                     /locus_tag="VNT12_04240"
     CDS             complement(4063..4491)
                     /locus_tag="VNT12_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI45361.1"
                     /translation="MDYFVWAAVSVEHTVVVDAGFSAEVAEKRQRTHLRCPTDGLAAL
                     GIDCARLPLVVLTHLHYDHVGNAERRVGLRYFGHSAFLWSTPSGVRVLIDPFGNADEP
                     RSSPSFEFVPIPSVELSVSDLPGEREVWTFEGLSDVNEPG"
     gene            5236..6252
                     /locus_tag="VNT12_04245"
     CDS             5236..6252
                     /locus_tag="VNT12_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI45362.1"
                     /translation="METIAQLEKKLAEPSEDLTEDIAALEGDVLILGVSGKVGPGLAR
                     QARNAIDKAGVDKRVIGVARFTDKGLAEELQRDGIQTIAADLTDDRQLQELPDVENVV
                     YMIGNKFGTSGNEHFTWMMNAYLPGRVAERFRNSRIVAYSTLLVYPLTPVMLGGSMEH
                     HAPGPVGEYAQSCLGRERVFEHFSRKHGTPVVLFRLAYAIETRYGVLLEIAQAVKSGE
                     PIDLRMGHVSVIWQGDATELALRSLNVCDSPPRVVNVSGPELVSVRWLAERFGERLGT
                     APVFSSEEAETSFVINASQAHRLFGYPKVDLPEMIEWVAQWVESDGETANKPTRFQQR
                     EGYF"
     gene            complement(6303..7103)
                     /locus_tag="VNT12_04250"
     CDS             complement(6303..7103)
                     /locus_tag="VNT12_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45363.1"
                     /translation="MSHESLGGSRARGWPREVRVQVPSSSDRQRGQGTLLGRAVLAFW
                     SDVAPEHEDGFNDWYTNEHLPERVGVPGFLRGRRYVKTDDSQAVQKYFTLYETESLAT
                     LSSASYLERLDNPTPWTQKMVPLFLNSNRVAYAIGASIGGGIGGWMATVEFGPQPDAA
                     DELRSSLAQRTLPSLLDEPDVTGCALGEADLDTTSAKDGTAEGRDADGGSEVAASWIL
                     FVEGARSSVVDIAEARLLGPDGLSRHGALEEVALKPYRLMFSLADRPI"
     gene            complement(7266..8360)
                     /locus_tag="VNT12_04255"
     CDS             complement(7266..8360)
                     /locus_tag="VNT12_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HWI45364.1"
                     /translation="MVFRLGVEDFFPGGLTSTEGLRVAQWAARAGADALSITAGHYRS
                     LPAAERMIPPMAYPEGTFLAFAARVKEEVEVPVVGVGRLGNPAVAGAAVEEGKTDMVA
                     LGRTLIADPDWPNKVRSGAAARRCLSCNHCVNGMRSGSHISCVVNPSAGFELDFADPA
                     PPEGEMICIVGAGPAGLSYASLVADRNRVTVLERAPVSGGAFRYTGKAPLFGEVEAAE
                     ESFGTYVAELERACRDKGVGFRHGVDVTEEPQALAPYDRVVFVTGARYRYGLGTLATR
                     LLDAGLGKSKLGRRLFASPRLRNWLYYGARRGTGEDLRRLARTDQEVVVIGDAAQAGK
                     ARDAIDGAFRAALFPEHEQRADIHALSDRR"
     gene            complement(8388..9866)
                     /locus_tag="VNT12_04260"
     CDS             complement(8388..9866)
                     /locus_tag="VNT12_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HWI45365.1"
                     /translation="MTAEYQMLIGGGWVDGSTGERLASINPFNQEAWATIPQASEDDA
                     NRALSSAREAFENGWGDVNGLRRAQLMTKLADLIEANASRMARLETKDNGKVIRETQN
                     QVRFSARNYRFFAGYADKLYGEVIPLDNTDLFDYTLREPFGVVVLIIAWNSPMQLLAN
                     KLAPALAAGNTVVVKPSEHASVTTLELGKLVEKAGFPDGVVNIVTGNGRVGDFLTRST
                     SVDKISFTGGAQTGRIIASNASQNLVPTTLELGGKSPNIVFADADLERAINGAVAGIF
                     GAAGQTCIAGSRLLIQRPVYGEVVDEVASRARKICLGDPLNPDTEMGPVANEAQYERI
                     LELIDAGKVEGAEVAAGGEPVRGDDFDRGFFIAPTVFANVDNRMTIAQEEIFGPVLST
                     IPFDDEEQAIEIANSTRYGLASGLWTRDFSRTHRFARSIHAGTVWVNTYRGAAAQAPF
                     GGVKQSGYGRERGQHALVEYTRVKNVMADLSQSTSDPFSIRS"
     gene            complement(9900..10697)
                     /locus_tag="VNT12_04265"
     CDS             complement(9900..10697)
                     /locus_tag="VNT12_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI45366.1"
                     /translation="MPYTTVDDVRLYFEEVPNSDAPPLLLIHGAAQDTLSWRFNLDFL
                     GSKFHVYAIDLPGHGKSQMAPEGPIDSLEAYAAYAERFMTRLGVAHYSVVGHSMAGGI
                     GLHMALRHPERLDMVALLDGACYTSGTYGEEVFDLVSINPTDWFEVNFRTICSPKTSP
                     QRVEEIAFDVRRCAPEVAMNDIRAYAALDLRERVGNVTIPVVAIHGEEDWSIPPELGK
                     RTTELTGGPSLFLPLGGVGHFPHTEAPELFHAAFEKALETIDVRFGR"
     gene            complement(10699..>11243)
                     /locus_tag="VNT12_04270"
     CDS             complement(10699..>11243)
                     /locus_tag="VNT12_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45367.1"
                     /translation="TGLVRQMYRIAMSEGLSSEDIVAVIKLFEKWSGVQARGAARREA
                     SGGEHEVEAVGEGPNLTTELLRRIQAKLNEDPVVVSNARWYRATLRLVCDEHSWDYRV
                     ADGRVQLVDDGGSSQDPQVRIRGSSEDWRPVLRGLRGGLHRAFRHRLLTFDGDAVATL
                     MLWKTIWRLGEALAEAGQEG"
BASE COUNT         2099 a         3606 c         3510 g         2028 t
ORIGIN      
        1 acaaccacat cccgccaggc gagccaatcc tcttccttga ggtcgacgtg gaggacgctt
       61 cagagttcga ggacgtgctc gaggtgaggg gtgtggagat cggaggccac aaggtgctcc
      121 gcgccaagag ttcggtggtg cccaacgcca tcgccgccct cttgctccac ctgcgggaca
      181 ccaccggcaa gaccccgcac tgctacttcg ggtgggcaga gggcaatcca atcgcgtacc
      241 tgttccgctt cgtcttattt ggggagggag acaccgcgcc ggtcacccac gaggttttga
      301 gggaggccga gccggagcgc aaacggcgac ccgccattca tgtcggcggt aggtgaacga
      361 cactctgcac gtcaaactcg ctgtgtgctc gctcaagacg cccaccttct tcaggcctct
      421 cgggtgcgcg ggggaggcgc cggcggggca ggtcgctact gccttcgtct gggtgaacgc
      481 cgtatgtagc gcgttgggga ccggggcggg cttctcctat accactatca tgagagctga
      541 aagcaccgcc gcaccaccga ctacgaggag gatacctaga agtctcgacc gcccggcccg
      601 atgccatcgt ttcgctgtct tcatgtgcgc tatccttcca cccgcttccc gtggggcaaa
      661 ccctttccac cgcgcccaca ttagcaccaa acgtgacaag aatgcttcgg cgatcctatc
      721 ctgtcaactc ctcagacccc cctacacgtg aacttcggag aacgcctctt ccacacaatc
      781 gggtgaataa aggccgagac ggccgattga cgtcagttcc tattggtccc actggtccgg
      841 ctaaagatca gagctagcag caaacaaaca acagggaaaa cccctgcgta gcctgctatc
      901 cctattcctc gcaactgcat gtctccgagt gccgcaccga gaagaccggc gatggagagc
      961 gtgccgctca agatcatgca ggctcggacg gaggtatgca gcttcccgcc ttcgaatacg
     1021 ggggcggcca ggagcataga cagaccgaag aaccagtccc aggccactat gtccagtgcg
     1081 taatctacgg agggccactc ccaggagaag agccggaagt atgcgggcat gctcgtggga
     1141 tcaattcggc gagctaccgt caattctaca aagtggacgc acgtcgtcag gcctgcacac
     1201 aggatcatga agccaagcgc cgacaagcta tacatcttgg tgccgcgcgg cgcatagccg
     1261 tgcacggcga ccatcgtaag gaccataaac ggggccatca ggatgatctg tacctccaag
     1321 atccctaaga acggatccgt taagggatct gtcacgttcc ctgccgagac aaacgccgca
     1381 acaagggtcg gggtatacaa gaggccgaag aagcaggtgc ccaaggccga ccagatggcg
     1441 acccggcggt ctcttgcggt ccagtcgttc atgagtgaca cgctcaaagc cctgggggaa
     1501 tagcgccgat attgaccctc acgtacacga caagcctcct tttcaccgat gagcatgatg
     1561 ccatccaccg gagctcgggg cgaacgtcgg agaatccacc ttccccgacg ttggatgaat
     1621 aagccaccga ggcattagta caaggctaaa ctcttcggct atggaccgaa tcctctctct
     1681 catagagcac tacggctacc tggtcatcct cctcggggtg atgcttgaga gcaccggggt
     1741 gccattgccc ggcgagacca tacttctcgc ctccggcgtc ttggtccagc ggaggcaccg
     1801 cgacctcggg gatgccatcg tgttcggcat cctggggtcc gtgttaggtg accagatcgg
     1861 ctactgggca ggccgtgaag ggggacgccc gttcgtgctg cgttggggcc gctacgtctt
     1921 catcaccccc gagaggttgg cacgcgccga agcattcttc gagaggcacg gggggaaggc
     1981 tgtattcctt gcacgcttca tctccggctt gcgcgtgttc ggtgccctgg tcgccggcat
     2041 aagccggatg cgctggggga ccttcatcct ctacaacgcc ttgggtggcg tcgtctgggc
     2101 tacggcggtg gtgctggttg gctacttcct tgggagcagc attgggctgg tggagcgttg
     2161 gctgggagga gctacgctgt tgctcctcgc agtgctcgcc gtggtggtcg tcttctatct
     2221 cgcataccgc tgggccgcgc acaaccggtc gcggctggtg gggtggggcg atgcgctgct
     2281 ggcctaccct cccgtggacc gcttgcgaac gcgacgcgcc tagagcgtag cttctcctcc
     2341 gccccgacca ggtccacctc cacctcgccc ttcctcgtcc agtatccgcc cacgtactgc
     2401 gcatccccga agcgctcgtc gggcagcatg cggaagacgg actcccggac caggtgcccc
     2461 acggcgttgc cgcggtacgt ggaccagctc tcccggattc tcgcgaccac gtgctcgccg
     2521 cgaccgcgct ctatctcggc catgttgagc cctacgaagc gtagccagaa gcgaaggtag
     2581 gggtctgcga ccgcgtagtg cgtctccctc gaagccctgc tcgagagcgg gcgctcggcc
     2641 gacaccacgc gcttctgccc gaccaacacc tcaagcgccc tctgcagggt cgtctcgtgg
     2701 atgcccgcgc gacgctgtat cgtggtgaag gttctctctc cggagccgat ggtcgcgagc
     2761 acctctgccg ggccctggcc tcctccggaa actccgcaga taggatgcgc tccccgccaa
     2821 cgatgagcgg cgaggtgggg ttggagatct gcccttccag gaaatcccac atgccagtcc
     2881 cggcccgcca ctcttgggtg atctggggaa acccacccag caccaattgg gcatcgatcg
     2941 cgtcggcggc cggcagatcc agcatctcgg ccgtctcgtg cggcccaaag ggctccacga
     3001 ccatctccag ggtcggcctc ccgtaaagcg gccggtcata atgcgtcagc gccgacatca
     3061 tggaaatgtc ggaacctatg agcacgagga gtaccggagc ctccctgaga tacctgtccc
     3121 agaccttctg cagggtcgcc tccagggaag ggtcttgctc caagaggtag ggaaactcgt
     3181 ccatcacgat gacttgcggt gttctgccac gggtagcacg caaattcccc atcgacgaac
     3241 cgggcgcgat ttttgacggg agcgtgacgg gagtaggacg ggaacaggat agaacaaacc
     3301 gatgccccga agcacaaaaa gtgcttagaa atcgccctta gcacacttcg gagaaccgta
     3361 tagaaccgcc cgattcggcc tacgaatcag aaggtcgcag gttcgagtcc tgccgagcgc
     3421 gccccgctaa ttcctgtatt tgcaggagtt tttgttccaa ctcagccttc ggctccgcca
     3481 acttatccgg gtctgacagg aacgtgacgg gagtagagcc ccggttgggc cgttcgaaac
     3541 cccgaaaagc ggctctccga gggtctccgc agcctgctct aagaagtcac tcaggaaatc
     3601 cggcacagcg ggcccagaga gggaagactg aagggcgtgc ggggccacca ggacaccagc
     3661 ctggacaagg tgctggagac cgcgatcctc gagaagatcg agcgggtcgc gtaggcgctc
     3721 gccagcggca aggaggtcat ggacgcgaag gaggcggcgg acttcctgag gataagcgaa
     3781 tgggagttca agaggatggc accggggtta ccccgacatg ccgttaccga gcggcggtac
     3841 atctacctgc ggagcgaact cttgacatgg ctcttgggac ggcaaagacg acgcgtggcc
     3901 tcaagcgctt cgtcaagaaa ggcggcccgt cgccgcacga cacagtatgg agtggtgtcg
     3961 gatcgttgtc gacggggacg aaggcgcctc agtagccgcg gctctactgc gcggtacggc
     4021 ggcgaaggcc tcttcgctcc cgtctgttgc gccaggacgg ctctagccgg gctcgttcac
     4081 gtcggagagc ccctcaaacg tccaaacctc gcgttctccg ggcagatcgg agaccgaaag
     4141 ctccactgac ggaattggga cgaactcgaa agaaggggaa ctgcgcggct cgtcggcgtt
     4201 tccgaagggg tcgatcaaga cgcgtacgcc ggagggcgtg ctccacaaga aggcggagtg
     4261 accgaagtag cggaggccga cccgccgttc tgcgttgcct acgtgatcgt agtgcaggtg
     4321 agtcaggacg acgaggggca accgtgcgca gtcgatcccc agggccgcaa gcccgtccgt
     4381 aggacaccgc aggtgcgtcc gctgcctctt ctcggccacc tccgcggaga aacccgcgtc
     4441 gacgacgacc gtgtgctcga cggagacggc agcccaaacg aagtagtcca tgggcatcgg
     4501 accgtcatgc gggtccccgc cgtggaagtg ctccgggctg cgcgcctcgc gtactttacc
     4561 gcgtacacct ggtacagggg cagattctcc tcatccgaat atcctggcaa cccgccgggc
     4621 tccagaagac gctgcgtcac cgcgcccacg cgaggttacc ccccaccggg ccccgatcgg
     4681 gcctcggtgg ttggctcgat accttcggcg cccaacccgc tcgcgggcgg cgagcgccgc
     4741 gcagaaggct gcatttccgg cgcccgcaac gaccacatcc tatccttgct tcatgagact
     4801 cccccgaagt atccgagaag tattcacaag gggacgagta gaagacggcg gagaaccgtc
     4861 ggtggtggct gcgcaggaga tgcgacagtc ggctgctcgt ggggcccgac gcgcattgcc
     4921 agaccggcgg gtgtagactg aagaagagtg aggaagggag gaaggtggca acgccggtgt
     4981 cggtcggcac caccgccggc tcgggaggtc tctgtcgcag ccactatgca cgtacgacac
     5041 gagatgccgc tatagcagcc gaccggcctg ctcttcgcta cgtttttggt agcgcgaggt
     5101 ctgacgcacg ctggagtcag aacacgaacc ggtgtcctgc cgggcttcgc tgaggatagc
     5161 gacgcgcgaa ggggaaccag aacgctagct ccccaacccg agttcggaga cggtcgatct
     5221 ttgtcggagg tttacgtgga aaccattgcc caactggaga aaaagctggc cgagccttcc
     5281 gaggatctca cggaagacat cgccgcgttg gaaggcgacg ttctgatctt gggggttagc
     5341 ggcaaggtgg gtcccggctt ggcccggcag gctagaaacg ccatcgacaa ggcgggggtc
     5401 gacaagcggg taataggggt cgcccgcttt accgacaagg gtttggcaga ggagctacag
     5461 agggacggaa tccagacgat cgccgcagat ctaacggacg atcgtcagct gcaggagctg
     5521 ccggacgtcg aaaacgtcgt ttacatgatc gggaacaagt tcggcaccag cggaaacgag
     5581 cacttcacct ggatgatgaa tgcctacttg ccgggcaggg tggcagagag gttccgcaac
     5641 tctcgcatcg tggcttactc gacgctgctc gtgtatccgc tgacgccggt catgctcggg
     5701 ggctctatgg aacaccacgc tcccgggccc gtcggggagt acgcgcagtc gtgcctcggt
     5761 cgggagcgcg tcttcgaaca cttctcccgc aagcacggca cgcccgtcgt gctcttccgt
     5821 ctggcgtacg ccatagagac caggtacggg gtgctgctcg agatcgcgca agcggtgaag
     5881 agcggggagc cgatcgacct gcgtatgggt cacgtcagcg tgatctggca aggagacgcc
     5941 accgaactgg ccttacgttc cttgaacgtg tgcgactctc ctcccagggt cgtgaatgtc
     6001 tcgggaccgg agttggtctc cgtgcgttgg ctggccgagc gtttcggtga gcgcctcggc
     6061 accgcaccag tattttcgtc agaggaggcc gaaacttcgt tcgtcatcaa cgcctcgcaa
     6121 gcccacaggt tgttcggata cccgaaggtt gacctgccag agatgatcga gtgggtggcg
     6181 cagtgggtcg aatcggacgg tgagaccgcc aacaagccga cgcgttttca gcagagggag
     6241 ggatatttct agaggcgtgc gactttctgc ggcgaccggg atcggtgcgg ccgttggcga
     6301 ggttagatcg ggcgatcggc cagggagaac atgagtcggt atggtttgag tgcgacctcc
     6361 tccagggctc cgtgccggga gagtccatcg ggtcctagca atcttgcttc ggcgatgtct
     6421 acaacgctgc tccgggcgcc ctcgacgaac aggatccaac tcgcggcgac ctctgatcca
     6481 ccgtccgcgt cccgaccctc tgcggtgccg tccttggccg acgtggtatc gaggtccgct
     6541 tcgcccaacg cacaacccgt gacatcgggc tcgtcgagca agctcgggag tgttcgctgc
     6601 gctagcgacg accgaagttc gtcggcggcg tccggctgcg gcccaaactc gaccgtcgcc
     6661 atccacccgc cgatcccgcc gccgatcgaa gccccgatcg cgtaggccac ccggttgctg
     6721 ttcagaaaca acggaaccat cttctgggtc cacggagtcg ggttgtccag ccgctcgagg
     6781 taagaggcag agctcagcgt ggcaagggac tcggtctcgt atagcgtgaa gtacttctgg
     6841 actgcttggg aatcgtccgt cttgacgtag cgccgtccgc ggaggaaccc cggcacgccg
     6901 acccgctcgg gcaggtgctc gtttgtgtac cagtcgttga acccgtcctc gtgctcagga
     6961 gcgacgtcgc tccagaacgc gagtacggcc cgcccgagca gcgttccctg gccgcgttgc
     7021 cgatccgacg aagaagggac ttgtaccctg acctcccttg gccaaccccg tgcgcgactt
     7081 cctcccagag actcgtgcga cacggaagat ttcctatgtt tgatgtcgga ccgatagtat
     7141 catacgtagc ggggatcttc cccggtcttg gagccttcat cgcgaacctc gggcgcccgc
     7201 aggcacgcgg cacccgcgcc cttcgacccg gagatcgcag aggtagtcgg ccctccctta
     7261 acctttcatc ggcggtcgct caaagcgtgg atatccgcgc gttgttcgtg ctccgggaac
     7321 agcgcggcgc ggaaggcgcc gtctatggcg tccctggcct tgccggcttg ggctgcgtcg
     7381 ccgatgacca cgacctcttg gtcggtccgg gcgagccgcc gcaggtcttc tccggttccc
     7441 cgtctagccc cgtaatagag ccagtttcgt aggcgcggcg aggcgaacaa ccgccgtccc
     7501 aacttcgact tgccgaggcc cgcatccagg aggcgcgtcg cgagagtacc cagcccgtac
     7561 cggtagcggg ccccggtgac gaacaccacg cggtcgtagg gcgcgagggc ctgcggctcc
     7621 tccgtcacgt cgacgccgtg gcggaacccg acccccttgt cccggcacgc ccgttccaat
     7681 tccgcgacat aggtcccgaa cgactcttcc gcggcctcta cctccccgaa gagcggggcc
     7741 ttgcccgtgt agcggaaggc cccgcccgat acgggagcgc gctccagcac ggtcacccgg
     7801 ttgcggtcgg ccaccaggga ggcgtaagag agccccgcgg ggccggcccc cacgatgcag
     7861 atcatctcgc cctcgggggg cgcgggatcg gcgaagtcga gctcgaagcc ggccgagggg
     7921 ttgaccacgc aggatatgtg cgaccccgag cgcatgccgt tcacgcagtg gttgcaggat
     7981 aggcaccggc gtgccgccgc gcccgagcga accttattgg gccagtcggg gtcggctatc
     8041 agggtgcgac ccagcgcgac catgtccgtc ttgccctctt cgaccgcggc cccggccacc
     8101 gcagggttcc ccaggcggcc caccccgacg accgggacct cgacctcctc cttcacacga
     8161 gcggcgaagg caaggaacgt cccttcgggg taggccatcg gcgggatcat gcgctccgcc
     8221 gccggcagcg agcggtagtg accggcggtg atgctcaagg cgtctgcccc tgctcgcgcg
     8281 gcccactgcg cgacccggag gccttccgtg gatgtgagcc caccagggaa gaaatcttcg
     8341 acgcccagcc ggaagaccac cgggagatcc ggcgtaaagc aacggggtca agagcgaatg
     8401 gagaagggat cgctggtgct ttgggagagg tcggccatca cgttcttgac gcgcgtgtac
     8461 tccacaaggg catgctgacc tcgctctcgg ccgtacccgc tctgcttcac cccgccgaag
     8521 ggcgcttggg cagcggcacc ccggtaggtg ttcacccaga ccgtgccagc gtggatcgag
     8581 cgcgcgaagc ggtgtgtcct cgagaagtcg cgagtccaca aaccggaggc tagaccgtat
     8641 ctggtcgagt tggcgatctc gatagcttgc tcctcatcgt cgaaaggaat ggtgcttagc
     8701 acgggcccga agatctcttc ttgagctatg gtcatccggt tatcgacgtt ggcgaacacc
     8761 gttggagcga tgaagaatcc tctatcgaag tcgtccccac ggacgggttc gcctcccgcc
     8821 gcgacctccg ccccctctac cttacctgcg tcgatgagct cgagaatcct ctcgtactga
     8881 gcctcgttgg ctaccgggcc catctccgtg tcaggattaa ggggatcgcc gaggcagatc
     8941 ttccgggcgc gagacgcaac ttcgtccacc acttcgccgt agacaggacg ctggatgaga
     9001 agcctagatc cggcgatgca ggtctggccc gcagcaccga agatgccggc cacggcacca
     9061 ttgatcgcac gctcgaggtc agcgtcggcg aacactatgt tcggagactt cccgccgagc
     9121 tccagggtag tgggtacgag gttctggctc gcgttgctcg ctattatgcg acccgtctgc
     9181 gcccccccgg taaagcttat cttgtccacc gaagtgctcc tggtgagaaa atcaccgacc
     9241 ctcccgttgc cggtcacgat attgactacg ccatcaggga atccagcttt ttcgacgagc
     9301 ttgcccagtt cgagggtggt gaccgaggcg tgctctgaag gtttgaccac caccgtattg
     9361 ccagcggcca acgcaggcgc cagcttgttg gccagaagct gcatgggcga attccaggcg
     9421 atgatcaaaa cgacgactcc gaagggctcg cgcagtgtgt agtcgaacag gtcggtgttg
     9481 tccagcggta tgacttcgcc gtagagcttg tccgcgtacc ccgcgaagaa cctgtagttg
     9541 cgggcggaga agcgcacctg gttctgggtc tcccgaatga ccttgccgtt atccttagtc
     9601 tccaggcgag ccatccggga ggcgttagcc tcgatcaggt cggcgagttt agtcatcaac
     9661 tgggcccgtc tcaggccgtt gacgtcgccc cagccgttct cgaacgcctc ccgcgccgaa
     9721 gagagggcgc gattcgcgtc atcctcggat gcttgcggta tggttgccca cgcctcctgg
     9781 ttgaacgggt tgatcgacgc aagacgctcg ccggtcgaac cgtcgaccca ccctccacct
     9841 atcagcatct ggtattcagc cgtcacaact ctcctcctcg ccgctctagc cctcggtact
     9901 cagcggccga accttacgtc gatcgtttcc aacgcctttt cgaatgccgc gtgaaagagc
     9961 tcgggagcct cggtgtgagg gaagtgcccg accccaccaa gaggcaagaa gaggctcggt
    10021 cctccggtca gctcggtggt tctcttgcca agctcgggtg gtatcgacca gtcctcctcc
    10081 ccgtggatcg cgaccacggg tatcgtgacg ttcccgactc tctcgcgcag gtccaacgcc
    10141 gcgtacgctc ggatatcgtt catggccacc tctggcgcgc agcggcggac gtcgaacgcg
    10201 atctcctcga ccctctgggg gctggtcttc ggcgaacaga tggtgcggaa gttgacctcg
    10261 aaccagtcgg tagggttgat ggagaccagg tcgaacactt cttccccgta ggtgccggac
    10321 gtgtagcacg cgccgtccag caacgccacc atgtctagcc gctcggggtg gcgtagggcc
    10381 atgtgcaagc cgatgccccc agccatagaa tggcccacca ccgagtagtg agcaaccccc
    10441 agtctcgtca tgaagcgttc cgcgtaagcg gcgtacgcct ccaggctgtc aatggggccc
    10501 tcgggggcca tctggctctt cccgtgcccg ggcagatcga tggcgtacac atggaatttg
    10561 ctgcccaaaa aatccaggtt gaagcgccaa gaaagggtgt cttgggcggc cccgtggatc
    10621 aataacagag gaggcgcgtc gctgttcggc acctcctcga aatagagtct cacgtcgtct
    10681 acggtggtgt agggcacgtc agccttcctg ccccgcctcg gcaagagctt cgccgagacg
    10741 ccagattgtc ttccacagca ttagcgtggc cacagcgtct ccatcgaaag tcaagagcct
    10801 gtgccggaac gcccggtgca gaccgccgcg taaccctcgg agaacgggcc gccaatcttc
    10861 gctgcttccc cggatacgaa cctgaggatc ttgggatgaa cctccgtcat cgaccagctg
    10921 gacacgccca tcggctacgc ggtaatccca cgagtgctcg tcgcacacca acctcagcgt
    10981 cgctctgtac cacctggcgt tcgagaccac gacggggtct tcgttcagct tggcctgtat
    11041 ccgacgaagc agctccgtcg tcaaattggg cccctcgcca accgcctcga cctcgtgttc
    11101 gccaccgctg gcctcccgac gagccgcgcc tcgggcttgg accccgctcc atttctcgaa
    11161 gagctttata acggcgacga tgtcctccga ggagagtccc tccgacatgg cgatccggta
    11221 catctggcgc accaagccgg tcg
//
LOCUS       DATMJA010000180         3542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_319726, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000180 DATMJA010000000
VERSION     DATMJA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319726"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VNT12_04275"
     CDS             complement(<1..455)
                     /locus_tag="VNT12_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45368.1"
                     /translation="MWLLRRIGERLLGPVDRLQRAFHRSPPAFRRGAAMLVDALIVIE
                     SFAVTLIVRVSGDAVAPSWPFFWPFAVFSALAFVLLLNANGVYQSILRYTGIYQGVRV
                     LSATAIAAGGLFLAVFCVGPFGFGFMRGNLVPLSVPLVGSVLAYVQLVAV"
     gene            complement(493..1473)
                     /locus_tag="VNT12_04280"
     CDS             complement(493..1473)
                     /locus_tag="VNT12_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45369.1"
                     /translation="MTGVVAGGVAFLVAGAFVPLLVRFAVGRNLLDVPNLRSSHEVPT
                     PRLGGVAIFVGMLAGVALLRPEGLWPLLAAAALVWAVGLADDLSNLHFGVKAVLQALA
                     AVGLLLFYPPTILSDAPGVLGALVFVVGVFWIVALSNAFNFMDGIDGFTGGVALVNAL
                     FLAPLVGPAGGFLPALIGATAGFLIWNINPASIFIGDSGAYFLGFSLAGVAFYAPALS
                     GQEWTPLGFLACVVVFTPYLFDTAYTLVRRLRSGAGKSIFLAHREHIYQRITPTTGMH
                     RRTSNLYYGASVVAGFAALLISRGDALSLVGIALALACCALLAALPKLIS"
     gene            complement(1489..1653)
                     /locus_tag="VNT12_04285"
     CDS             complement(1489..1653)
                     /locus_tag="VNT12_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45370.1"
                     /translation="MCSRGVGVANLAITVDEEKVKRARTLWAGAGGSLTECVPHGLFV
                     EGMGIESPFA"
     gene            complement(1678..2556)
                     /locus_tag="VNT12_04290"
     CDS             complement(1678..2556)
                     /locus_tag="VNT12_04290"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWI45371.1"
                     /translation="MRILLTGATGLLGGALLRLLLARGHETRCLVRADSPNTSRLDGE
                     RVEIIHGDAAREEDLYRALRDADVLLHVAGIEYAPTVVRAAGRAGVERVLIVGSTSAH
                     SAYAFRSGPKLRMEELVRGSGLAWTIFRPSMIYGSERDRNVHRLLRFLDRSPVFPIFG
                     PGTNLWQPVYHEDCARGVFEALERPASVRRSYDLPGAEPLAYVDLVKTAAAALGRSPR
                     IVRLPIEPVRLALAAAERLRLPLPFDSGQVMRLREDKAYPYERASRDLDYAPRPFREG
                     VALEVARLRRLGMVRS"
     gene            complement(2605..3093)
                     /locus_tag="VNT12_04295"
     CDS             complement(2605..3093)
                     /locus_tag="VNT12_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45372.1"
                     /translation="MHQRGISTVLFLATIVLIAGLLTGCGGGDQSGSGSQQDGGSKAE
                     QGKNAPVEQGGNAPKKQGKNAPKKKIVFGTVTFVNPKTEGFSVRPTIEEQGKKPVVFR
                     LANETSITLDGKKAKLEDMKKGQQAQVEYVERNERNRARSVKLFEAGGGSQDNQGGEK
                     TG"
     gene            complement(3156..>3542)
                     /locus_tag="VNT12_04300"
     CDS             complement(3156..>3542)
                     /locus_tag="VNT12_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWI45373.1"
                     /translation="DEFVRQRARMYNASFGKAWEAYEAHTGRKVATRYEDLRYDALGE
                     LEKIYSSLVIPIEEEQLRRVVEKYAWENIPERQKGPNKPRRKAKPGGWREDLTPEQAR
                     MVEEITTPIMDEFYPGWERAGATPSD"
BASE COUNT          653 a         1196 c         1083 g          610 t
ORIGIN      
        1 acggcgacga gctgcacgta ggcgaggacc gagccaacaa ggggcaccga gagcgggacg
       61 aggttgcccc tcatgaaccc gaacccgaac gggccaacgc agaacaccgc caggaagaga
      121 ccaccggccg ctatggccgt cgcgctcaga acccgcacac cctggtagat gccggtgtag
      181 cgcaggatgc tctggtagac cccgttggcg ttcaggagta ggacgaacgc gagcgccgag
      241 aatacagcga aaggccagaa gaacggccag gatggagcca ccgcgtcccc ggagacacgg
      301 acgatgagcg taacggcgaa agactcgatg actatgaggg cgtcgacgag catcgccgcc
      361 ccgcgcctga acgcgggcgg cgagcggtgg aaggccctct gcaaccggtc cacaggaccc
      421 aggagcctct ccccgatcct ccgcaaaagc cacatagtcc tacattctaa cttctatggc
      481 tggatccggc cattagctta tcagcttcgg tagtgccgca agcagggcgc agcacgccag
      541 tgcgagcgcg atgcccacca acgacagggc gtctcccctg gagatcagta gcgcggcgaa
      601 accggcgacg acgctggccc cgtagtagag attgctcgta cgcctgtgca tccccgtggt
      661 cggggtgatg cgctggtaga tgtgctccct gtgcgccagg aagatgctct ttccagcccc
      721 gcttcgcaac cgccgaacca gcgtatacgc ggtgtcgaag aggtaaggcg tgaagacaac
      781 cacgcaagcc aggaagccca aaggcgtcca ctcctgcccc gagagcgcgg gggcgtagaa
      841 ggctaccccg gccaggctga agccgaggaa gtaggctcct gaatccccga taaaaatgga
      901 tgcagggttt atgttccaga tcaggaaccc tgcagtagcc ccgatgagcg cgggcaggaa
      961 accacccgcc ggtcccacta gcggcgcgag gaagagcgcg ttgacgagcg cgacgccacc
     1021 cgtaaagccg tctatgccgt ccatgaagtt gaaggcgttg ctcagggcca cgatccagaa
     1081 caccccgacg acgaaaacca gggcgccaag caccccggga gcgtccgaga ggatcgtcgg
     1141 cggatagaac agaagcaatc ccacggcggc cagagcctgg aggacggcct tcacgccgaa
     1201 gtggaggttg gacaggtcgt cggccagccc tacggcccat accagggccg ccgccgcgag
     1261 cagcggccat agtccctccg ggcgaagtag cgccactcct gcgagcatcc caacgaatat
     1321 ggcgacgccc ccaagcctgg gtgtcgggac ctcgtgcgag ctgcgtaggt tgggcacgtc
     1381 gagcagattc cgtccgacgg cgaaccgcac gagcagtggt acgaacgccc cggcgaccag
     1441 gaatgccacg ccaccggcga caacgccggt catcgtagct cccgcctcct aagcaaacgg
     1501 gctctcaata cccataccct cgacaaagag gccgtggggc acgcattccg ttagcgaccc
     1561 gccggcgcca gcccagaggg tccttgcccg ctttaccttc tcttcgtcca ccgtgatcgc
     1621 caggttggcc acgccgactc ctctcgagca cacgatttca gcatagcacc ctacggacta
     1681 cgaccgcacc atcccgagtc tccgcaggcg cgcgacctcg agggcgacgc cctccctgaa
     1741 gggacggggc gcatagtcga gatcgcggct ggccctttcg taagggtagg ccttgtcttc
     1801 acgcaaccgc attacctgac ctgagtcgaa gggcagaggc agccgcagcc tctccgcagc
     1861 ggcgagcgcc aggcggaccg gttcgatagg cagcctgacg atgcgtgggc tcctacccag
     1921 tgccgctgcc gcagtcttca caaggtcgac gtacgccagg ggttcggcac ctggaaggtc
     1981 gtagctgcgg cgtacggacg cggggcgttc gagggcttcg aatacgccgc gcgcgcagtc
     2041 ttcgtggtag acaggctgcc agaggttcgt gccagggccg aagatgggga agacaggcga
     2101 gcggtcgagg aagcgaagca gacggtgcac gttcctgtcc cgctcggagc cgtagatcat
     2161 cgaaggtcga aatatagtcc acgcgagccc gctcccgcgc acgagttctt ccatcctgag
     2221 cttcggaccg gaacggaagg cgtaggccga gtgcgcactc gtgctgccga cgatgagaac
     2281 ccgttcgacg ccagcccggc cggccgcccg tacgacggtg ggagcgtact cgatcccggc
     2341 gacgtgcagg aggacgtccg cgtccctgag cgcccggtag agatcctcct ctctggctgc
     2401 gtccccgtgg atgatctcca ccctctcccc gtcgagcctg gaagtgttgg gactgtcagc
     2461 gcggacgagg cagcgcgtct catggccccg agcgagcagc agtctgagta acgcgccccc
     2521 cagcaagccc gtggcgcctg tgaggagtat tctcatccgc ccaagggacg tgctggctct
     2581 cgattgcgtc cttccctttg gccctcagcc ggtcttctca ccgccctggt tgtcctggga
     2641 gcctccgccg gcctcgaaca gtttcacgct gcgagcccgg ttacgttcgt tcctctcaac
     2701 atactcgacc tgcgcctgct ggcccttctt catgtcctcc agcttcgcct ttttgccatc
     2761 gagtgtgatg cttgtctcgt tcgcgagcct gaaaactacc ggcttcttgc cctgttcctc
     2821 tatggtcggc cgcacgctga acccctcggt cttggggttc acgaacgtga ccgtgccgaa
     2881 cacgatcttc ttcttgggtg cgttcttacc ctgcttcttc ggcgcgttcc cgccctgctc
     2941 caccggcgcg ttcttgccct gctccgcttt ggatccgccg tcctgctgcg acccactccc
     3001 tgattgatcg ccaccgccgc agcccgtgag gagtccggct atcaagacga tcgtggccag
     3061 gaaaagcacc gtggatattc ctctctggtg catcgagtct ccttgtcctg ccgtgtgctt
     3121 tgcggggcta aactaccaga tagggcgaaa ggccgtcaat cggacggtgt ggccccggca
     3181 cgctcccagc cagggtagaa ctcgtccatt atgggggtgg tgatctcctc caccatcctc
     3241 gcttgctccg gcgtcaggtc ctcccgccac cccccaggtt tggccttgcg gcggggttta
     3301 ttcggcccct tctgcctctc ggggatgttc tcccaggcgt acttctcgac cacccttcgc
     3361 agttgctcct cctcgatggg gataacgagc gaggagtaga tcttctccag ctcgccgaga
     3421 gcgtcgtacc tgaggtcctc gtacctggtc gcgaccttgc gccctgtgtg tgcctcgtaa
     3481 gcctcccagg ccttcccgaa gctcgcgttg tacatcctgg cccgctgccg cacgaactcg
     3541 tc
//
LOCUS       DATMJA010000181         3133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_734596, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000181 DATMJA010000000
VERSION     DATMJA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734596"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..868)
                     /gene="hypB"
                     /locus_tag="VNT12_04305"
     CDS             complement(191..868)
                     /gene="hypB"
                     /locus_tag="VNT12_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HWI45374.1"
                     /translation="MHRTAVKKVVQENVLDANDTLARANRDLFDRAGTYTINMMSSPG
                     AGKTALLERTLERLRGRLRLGVLEGDVQTTLDADRLARFHIPLVQVNTDPGFGGECHL
                     DANMVRSGLGELPLEDIDILIIENVGNLVCPAEFRVGEDVRVMVYSVTEGEEKPLKYP
                     LMFRSADLVLVNKVDLLEHLDFDLEQFLGNLDAVNPGIRSILTSARTGQGVDEWCDWL
                     GQRLENI"
     gene            complement(871..1209)
                     /locus_tag="VNT12_04310"
     CDS             complement(871..1209)
                     /locus_tag="VNT12_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HWI45375.1"
                     /translation="MHELSIAESVIRIASRQADGRRVTKVQMKVGYLRQVVPSALTFG
                     FGLLAEGTPVEGAELEMEQIPAEGRCRLCGVESRLGSFPLQCEECGGFDLEILKGEEL
                     MVESLELEEA"
     gene            1347..1574
                     /locus_tag="VNT12_04315"
     CDS             1347..1574
                     /locus_tag="VNT12_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HWI45376.1"
                     /translation="MKPIELYTTAGCPYSEAAREDLEWRGVDFIEYDVERDENARGRM
                     LELTGGNRTVPVIFEEGKPVQVGWMGRGCAV"
     gene            complement(1579..2559)
                     /gene="hemB"
                     /locus_tag="VNT12_04320"
     CDS             complement(1579..2559)
                     /gene="hemB"
                     /locus_tag="VNT12_04320"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWI45377.1"
                     /translation="MAFPQQRLRRTRRTAGLRGLVRETRLSPVDLVYPIFVTAGEDVR
                     NPVASMPGIFQLSINHAVAEALRAHDLGIQAVLLFGIPDQKDEAATGAYDPEGVVQLA
                     TRAIKEAAPELLVVTDVCLCEYTSHGHCGVVEKETGEVLNDVSLELLARTAASQAEAG
                     ADIVAPSDMMDGRVAAIRSELDNEGFENTPIMAYAAKYASAFYGPFREAADSAPQFGD
                     RRSYQMDPANAREALVETGLDIEEGADIVMVKPALPYLDVLRRVREATDLPVAAYNVS
                     GEYAMVKAAAQNGWLDEQRAVLEALTGIKRAGADIIITYHAPDVARWLSG"
     gene            complement(2561..>3133)
                     /locus_tag="VNT12_04325"
     CDS             complement(2561..>3133)
                     /locus_tag="VNT12_04325"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWI45378.1"
                     /translation="LQHHGLDLRAVPRDAKVAAIGPATAERIEQAGLRVDVVPVEYRA
                     EALIDALRAHPLAGERVLIPRARVAREILPEKFREAGAEVVVPPAYQSVPSSEGKEEL
                     SLRLQSGEIDCVTFTASSTVENFVGAFGAEEAARLLAGTGVACIGPITADTARKYGLK
                     VDAEGKEYTIPGLIEAITGLLAADPAKRGE"
BASE COUNT          557 a         1034 c          940 g          602 t
ORIGIN      
        1 gagctcccct ctgtcgagtg ctcttgctgc gcactcctca ccccaaagga atggccagtg
       61 attcagcgtg cagtttgctc cctgaatcgc gtactcgccg ctttccccat gtacccatta
      121 cccactaatg agtagcgggg gcaggataac agtaccccga ccttttgtaa acgtggagac
      181 gactcacgtc tcagatgttt tcgaggcgct gcccgagcca gtcgcaccac tcgtcgacgc
      241 cctggccggt ccgcgcgctg gtgaggatgg atctgatgcc cgggttgacc gcatcgaggt
      301 tcccgaggaa ctgctccaga tcgaagtcca ggtgctccag cagatccact ttgttgacca
      361 gtaccaggtc ggccgagcgg aacatgagcg ggtattttag cggcttctcc tccccctccg
      421 tgacggagta gaccatgacc ctgacgtcct cacccaccct gaactcggca ggacacacca
      481 ggttgcccac gttctcgatg atgaggatat ctatgtcctc gagcgggagt tcgccgaggc
      541 cggagcggac catattggcg tccagatggc actcgccgcc gaaaccaggg tcggtgttca
      601 cctgcaccaa cgggatgtgg aaacgggcca gcctgtcggc gtccagggta gtctgtacgt
      661 caccctccag gacgccaaga cgaagcctgc cgcgcaagcg ctccagggtg cgttcgagta
      721 gcgccgtctt tcccgctccc ggagagctca tcatgttgat cgtgtaggtc cctgccctgt
      781 cgaacaagtc cctgttggcc ctggcgagcg tatcgttggc gtcaagcacg ttctcttgga
      841 ccactttctt gaccgccgta cggtgcatcc ctatgcctcc tccaactcga gtgactcgac
      901 catcagttct tcccccttga ggatctccag gtcgaagcct ccgcattcct cgcactgcag
      961 agggaagcta cctagtcggc tctccacccc gcacaagcgg caccgtcctt ctgcaggtat
     1021 ctgctccatc tccagctcag cgccttcgac tggcgtgcct tcagccagga gcccgaaacc
     1081 gaaggtcagg gcggaaggca cgacctggcg cagatagcct actttcatct gcaccttcgt
     1141 aacccgccgc ccgtcagcct ggcggctggc gattctgatc accgactctg ctatcgagag
     1201 ttcgtgcaag cacccactcc tcacggcgca cagaggctgt ctccccgatc cctcctccat
     1261 cgtagcccat aaccatagtg ggaacaatcg cgagagggtc cttcgggaga tagaatcaat
     1321 gagtgaatgt ggagcgagga ggatcaatga aaccgataga gctctacacc acggccggct
     1381 gtccttacag cgaggccgcg cgcgaggacc tggagtggcg cggggtcgac ttcatcgagt
     1441 acgacgtgga gagggacgag aacgcccgcg ggcgaatgct cgagctcacc ggcggcaaca
     1501 ggacggtccc cgtcatcttc gaagaaggca agcccgtcca ggtcggatgg atgggcagag
     1561 gatgcgccgt gtgatacgtc accccgacaa ccatcgggcc acgtcggggg cgtggtaggt
     1621 gatgatgata tcggcgccgg cgcgtttgat gcccgtcagc gcttcgagga cggcccgctg
     1681 ttcgtcgagc cagccgttct gggcggcagc cttgaccatc gcgtactccc cgctcacgtt
     1741 gtaggcggcg acgggaaggt ctgttgcctc gcggacgcgc cgcaatacat caaggtaagg
     1801 cagggcgggt ttgaccatta cgatgtcagc gccctcctcg atatccaggc ccgtctctac
     1861 gagcgcctcg cgggcgttgg ccgggtccat ctggtagctg cgccggtcac cgaactgagg
     1921 cgcgctgtcg gcagcttcgc ggaaggggcc gtagaaagcc gaggcgtact tggccgcgta
     1981 cgccattatg ggggtgttct cgaagccctc attgtcgagt tcggaccgga tcgccgctac
     2041 gcgcccatcc atcatgtctg agggcgcgac gatgtcggcc cctgcctcgg cctgggaggc
     2101 tgcggtgcgg gccaggagct cgaggctgac gtcgttgagt acctcgcccg tctccttctc
     2161 gacaaccccg cagtgtccgt ggctcgtgta ctcgcacagg cacacgtccg tcactacgag
     2221 cagctcaggc gcggcttcct ttatggcgcg ggtggcgagc tgaacgactc cctcaggatc
     2281 gtaggccccg gttgcggcct cgtctttctg atccgggatg ccgaaaagga gaacggcctg
     2341 gatgccgagg tcgtgcgccc gcagggcctc tgcgacggcg tggtttatcg agagctggaa
     2401 gatcccaggc atcgaggcca cagggttcct cacatcctcc ccagcggtca cgaagatcgg
     2461 gtagaccaag tccacgggcg agagccgcgt ctcacgcaca agcccacgca ggcccgccgt
     2521 gcgcctcgtt ctcctcaacc tctgctgcgg gaatgccatc ctattcaccc ctctttgccg
     2581 gatctgctgc aaggaggcct gtgatcgcct cgattagtcc agggatagtg tactctttgc
     2641 cttccgcgtc gaccttgaga ccgtatttgc gggccgtgtc ggccgtaatc gggcctatgc
     2701 acgcgacccc ggtccctgcc aaaagccgcg ccgcctcctc agcgccgaac gccccgacga
     2761 agttctccac cgtggagctt gccgtaaacg ttacgcagtc tatctctccg gactgcaggc
     2821 gcagggataa ttcctcctta ccttcagaag aaggaaccga ctggtaggca ggtgggacga
     2881 cgacttcggc gcccgcctcc cggaacttct ccggcaggat ctcacgcgcc accctagccc
     2941 tagggatgag gacgcgttct ccagccagcg gatgcgcgcg gagggcatcg atcagggcct
     3001 ctgccctata ctccacaggg accacgtcga cccgcaaccc ggcctgctct atcctctctg
     3061 ccgtcgccgg gccgatggcg gctaccttcg cgtcccgtgg tacggcgcga aggtcgagcc
     3121 cgtggtgctg cag
//
LOCUS       DATMJA010000182         5411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_497713, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000182 DATMJA010000000
VERSION     DATMJA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497713"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VNT12_04330"
     CDS             complement(<1..269)
                     /locus_tag="VNT12_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWI45379.1"
                     /translation="MDIGERERAGRTPRDRITQSCCAPPRQISEEPPTRPREEVRAEK
                     ASLEGMITLPGGTFLMGTGDGQGLPQDGEGPVRRITLDPFYISAFA"
     gene            500..2101
                     /locus_tag="VNT12_04335"
     CDS             500..2101
                     /locus_tag="VNT12_04335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWI45380.1"
                     /translation="MSGPRTSEKEPVRAYRRRTPSGGAVSERAAREPPLRSLLESRAL
                     LVLLAIMLLGAVLRFYGLGFQSLWSDELASWDFSNRETISQVIGGVRSDDNPPLYFLI
                     LRFAQVILGDSEWALRFPSAFAGWLCIPAIYLLGKRLYSGREGIVAALFLAVFWAPIY
                     FSQEARVYSMLILFSILTSYFWWNIMIGLRYGRELPTREATLYIVCAVLCAYVDYFGL
                     ILVVLQGAALAVLAYGMLRKAMLLYVPVAVAYLPWLPSMVHQSPYGDQNGTRIADTTL
                     QVPPDYFQFLFGRSGLLSFAAWTLLAFLLIRGWDDLRRRRKRGVVPPGLLLAAWAVGP
                     FVVAFVASHSMLTNENLLVSLPAVYLLLARSVTRTFSGRAAAVFQGTVAVGLAAVGLA
                     YLLFSMDYYTTPTKEQFREAALYVVGHEDKNTLVVRCDTHDRLDYYLKTRETGRRNDA
                     EACQSSDFPKIENRVKEGDYKEVFHFISHEDPDQQMVSKFQRSFQPVRYERFDGAAVV
                     VYKVGPSAPEGLPQPEPPSNLRRQE"
     gene            2138..3058
                     /locus_tag="VNT12_04340"
     CDS             2138..3058
                     /locus_tag="VNT12_04340"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWI45381.1"
                     /translation="MESKESRVGLKGVLAAVLTPMDEDLNPDHLPFAVHCHRLLAAGC
                     HGLSVFGTTGEANSLSVDERLAALEALVESGVPPEKLLPGTGSCALTDTVRLSRAALE
                     AGTAGALVLPPFYYKGVGDDGLFRFFAEVVERLGDDSLRLYLYHIPQMTGVDLGLSLI
                     SRLIDAYPGVVVGTKDSSGDRERITALCREFPDFSVLAGTETLLLETLRSGGEGCISA
                     TVNVTSRLARRVHDTHAAGKHDEAEALQERLTQLRASIEAYPVIPALKQLMARLTGDK
                     QWRNLRPPLSSLDEKRTQDLLSSVHLAELL"
     gene            complement(3157..5148)
                     /gene="ligA"
                     /locus_tag="VNT12_04345"
     CDS             complement(3157..5148)
                     /gene="ligA"
                     /locus_tag="VNT12_04345"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWI45382.1"
                     /translation="MAGGVDVRGRVEDLREQVRYHNRRYHVDDAPEISDAEYDAIYRE
                     LEALEGEHPELITPDSPTQRVGGEPIEGFEEVRHSVPMLSLANARKIEDLREWDARVR
                     RLLGEDETPRYVTELKIDGLAVSLRYENERLVRGATRGNGVIGEDVTAQLRTIRSIPD
                     RLDDDPPEVLEPRGEAYIPIADFEKLNTELEAEGKKPYANPRNLAAGSIRQLDPKITA
                     RRPLTILLYGVGEGGEIFQSHSEMLAALKAYGLRVNPYEIHEDIDSVIEECERWAKER
                     ESIGYQIDGVVVKVDSREQQLALGSVARSPRWAIAYKFEPLAGRTKLLDVVVKVGRTG
                     ALTPQAVLEPVNVGGVVISRATLHNEDYVKEKGILIGDTVVVERAGDVIPQVVKAVTG
                     ERTGDEQAFEMPANCPVCNEPVSRPEGEAVTRCVNARCPAQALEHIIHWSSKSAMDID
                     GLGEKLATRLFDLGFISDVADVYDLEPEQLVPLEGFGEKSAQNLIRAIESSKDRPFSL
                     VLYALGIRHVGAVTASLIATRFGGEDLMRGVEVEQLTGIDGVGEVVARAVVEYFDLED
                     NRDLVGRLVERGLDFGREEGVPAEGPLSGKRVVITGTLDRPRNYYVGRLEAAGGTFTS
                     SVSKNTDYVLAGEDAGSKLVRAKELGVPVLDEARFEELF"
BASE COUNT          962 a         1755 c         1610 g         1084 t
ORIGIN      
        1 gcgaacgctg agatgtagaa agggtcaagc gtgattctac gtactggacc ctcgccgtct
       61 tgtgggagtc cctggccgtc gccggtaccc atcagaaacg tgccgccggg cagcgtgatc
      121 atgccttcca gagaggcttt ctcagcccgt acctcttcgc gcggcctcgt agggggctcc
      181 tccgaaatct gcctcggggg cgcacaacaa gactgcgtaa tcctatcccg cggcgttcga
      241 ccggccctct ctcgctctcc tatgtccatc gggcgtaaga tacctccttt attctcttag
      301 atctacgaga ttaccactta cggtgggtga ggctgggtgg caacacgagg tgtaggggcg
      361 gttcgtgaat cgccctggcc cgaccacccc taaaacggtt tgcctcgaaa cgagcaagac
      421 gttactgtat ctggaaaatc tgtttgacat cctacatcgt ccactgctcg taaccgtgcg
      481 tgaaagagga gcgcgtacag tgtcaggacc gaggacgagc gagaaagagc cggtacgcgc
      541 ctaccgccgc aggacaccct ccggaggggc agttagcgaa agggctgctc gcgaaccgcc
      601 cctacgctcc ttgttggaga gcagggcgct tcttgttctg ctggcgatta tgctcctcgg
      661 tgcggtgttg cggttctacg gcctgggctt ccagtcgctc tggagcgatg aactggcgag
      721 ctgggacttc agcaataggg aaacaatctc ccaggtcatt gggggcgtgc gaagcgacga
      781 caaccctccc ctttacttcc tgatcctccg cttcgcgcag gtgatcctcg gcgattcgga
      841 gtgggctttg aggtttcctt ctgccttcgc ggggtggctg tgtataccgg ccatctacct
      901 cctcggcaag aggctctact ctgggcgcga ggggatcgta gcggcgctct tcctcgcggt
      961 attctgggct cccatctact tcagccagga agcccgcgtt tactcgatgc tgatcctgtt
     1021 ctccatcctc acctcctact tctggtggaa cattatgatc ggcctgcgct acggtcggga
     1081 actgccgacc agagaagcca ccctttacat cgtctgcgcc gtactctgcg cttacgtgga
     1141 ctacttcggg ttgatccttg tcgtgctgca gggcgcagca ctagcggtcc ttgcgtacgg
     1201 catgctgcgg aaagcgatgc tcctttacgt ccccgtagcc gtcgcttacc tgccctggct
     1261 gcctagcatg gtccaccagt ctccgtacgg agaccagaac ggaacccgga tcgcggacac
     1321 taccctccag gtcccgcccg actacttcca attcctgttc ggccgctcgg ggttgctctc
     1381 gttcgccgcc tggacactgc tcgccttcct cctcattcgc ggttgggacg acctgcggcg
     1441 tcgcaggaaa agaggcgtag tccccccagg cttgctgctc gcagcgtggg ccgtgggacc
     1501 gttcgtcgtg gccttcgtgg cgtcgcactc tatgctcacg aacgagaacc tccttgtctc
     1561 acttcccgcc gtctacctcc tcctcgcgcg ctccgtaacc cgcacgttct cgggcagggc
     1621 ggcggcggtc ttccagggaa cagtggccgt cgggctggcc gcggtgggcc ttgcgtacct
     1681 gctcttctcg atggactact acactacccc gaccaaggaa cagttccgcg aggccgccct
     1741 ctacgtcgtc ggccacgaag acaagaacac cctggtcgtc cgctgcgaca cccacgacag
     1801 gctagactac tacctgaaga ctagagagac gggccggagg aacgacgcgg aagcctgtca
     1861 gtccagtgac ttcccgaaga tagaaaacag ggtcaaagag ggagactaca aggaggtctt
     1921 ccatttcatc tctcatgaag atccggacca gcagatggtc tccaagttcc agaggagctt
     1981 ccagccggtc cgctacgaac ggttcgacgg cgccgcagtg gtcgtctaca aggtcgggcc
     2041 gtccgcgccc gaaggcctgc cgcaacccga acctcctagc aacctccgga gacaagaatg
     2101 accccgtacc aggtatggac caggtcgaga ggagaccatg gagagtaaag agagcagagt
     2161 aggcctgaag ggtgtcctgg cggccgtcct caccccgatg gacgaggacc tgaatccgga
     2221 ccatctgccc ttcgctgtcc actgccacag gctgctcgcg gcgggctgcc acggactctc
     2281 ggtcttcggc actacgggtg aggcaaattc cctctccgtg gacgagcgcc tcgcggcgct
     2341 cgaagcgctg gtcgaaagcg gtgtcccccc agagaagtta ctgccaggga ccgggtcctg
     2401 cgccctcacc gacaccgtac ggctgagccg cgcggcgctc gaggctggga ctgcgggtgc
     2461 gctcgtactg ccaccgttct attacaaggg tgtcggcgac gatggtctgt tccgcttctt
     2521 cgccgaggtc gtcgagcgcc taggcgacga cagtttgcgc ctctacctct atcacatccc
     2581 gcagatgaca ggcgtagacc tcgggctgtc cctgatctca cgtctcatcg acgcctaccc
     2641 gggcgtggtg gtcggtacga aggacagctc gggcgacagg gaacgcatca cggccctctg
     2701 ccgggagttt ccggacttct ccgtcctcgc cggcacggag accctactgt tggaaacgct
     2761 gcgtagcggc ggtgaaggct gcatctccgc gacggtcaat gtcacctccc gtctcgcccg
     2821 ccgcgtacac gacacgcatg cagcggggaa acacgacgag gcggaagcct tgcaagaacg
     2881 cctcacgcaa ctgcgcgcgt ccatcgaagc atatcctgta ataccggcac tcaaacaact
     2941 catggcccgt ctgaccggcg ataagcaatg gcgcaacctt cgtccgccgc tctccagcct
     3001 ggacgaaaaa cgcacacagg acctcctctc cagcgtgcac ctagccgaac tgctatgaaa
     3061 gcgtcgggta tgagccccgg ctaactggcc aaccggcggc gcagccgcca tgctgaccag
     3121 cgcgggtttt ggaagggtta actcttccaa aacccgctaa aacaactctt cgaatctggc
     3181 ttcgtcgagc acgggaacgc ccagttcctt tgccctcacc agcttcgagc ctgcgtcttc
     3241 gccggcgagc acgtagtctg tgttcttgct gactgaggag gtgaaggtgc cccctgctgc
     3301 ttcgagacgg ccgacgtagt agttgcgggg acgatccaga gttcctgtta taacgacgcg
     3361 cttgcccgag agcggaccct ccgcggggac accctcttcg cgcccgaagt cgagtccccg
     3421 ctccacaagg cgtcctacga ggtcgcggtt gtcttcgagg tcgaagtatt ctacgacggc
     3481 gcgggcgacg acctctccga cgccgtcgat acctgtaagc tgttccacct cgacgccgcg
     3541 cataaggtcc tctccaccga agcgcgtggc gataagactg gccgtaaccg ctcctacatg
     3601 cctgatgccc agggcataga ggacgaggga gaacggcctg tccttactac tctcgatggc
     3661 ccggatcaaa ttctgcgcgc tcttctcccc gaagccttcg agcgggacca gctgttcggg
     3721 ctcaaggtcg tagacgtcgg ccacgtcgct gataaagccg aggtcgaaca gccgggtggc
     3781 aagcttctcg cccaggccgt cgatatccat agcggactta gaggaccagt ggatgatgtg
     3841 ctccagagct tgcgcggggc aacgggcgtt cacgcaacgg gtaacagcct ctccctcggg
     3901 gcgcgagacc ggctcgttgc agacagggca attggctggc atttcgaagg cctgttcgtc
     3961 gcctgtgcgt tcccctgtga cggctttgac gacctgcggg atcacatccc ccgcccgttc
     4021 gacgacgacc gtgtcgccga ttaggatgcc cttctccttg acgtagtcct cgttgtgaag
     4081 cgtcgcccgt gagatcacga ccccaccgac gtttacgggc tcgagcaccg cctgcggcgt
     4141 cagtgcgccg gtcctgccga ctttcacgac cacgtcgagc aacttcgtgc gccctgcgag
     4201 cggctcgaac ttgtacgcga ttgcccaccg cggcgagcgg gctacagagc cgagggcgag
     4261 ttgctgctcg cgcgagtcaa cctttaccac gacgccgtcg atctggtagc cgatggactc
     4321 ccgctccttc gcccaccgtt cgcactcctc gatcacggag tctatgtcct cgtggatctc
     4381 gtaggggttg acgcgcagcc cgtaggcctt tagcgcggcg agcatctctg agtggctctg
     4441 gaagatctca cccccctccc cgacgccgta gagcagtatg gtaaggggcc ttcgggccgt
     4501 gatcttcggg tcgagttgcc tgatggagcc ggccgccaga ttgcgcgggt ttgcgtatgg
     4561 tttcttgccc tctgcttcga gctcggtgtt cagcttctcg aagtcagcga tgggaatata
     4621 cgcttcaccg cgcggctcga gcacttcggg cggatcgtcg tcgaggcggt cggggatcga
     4681 acgtatggtc ctcaactgcg ccgtgacgtc ctctccgatg acgccgttgc cgcgtgtggc
     4741 gcccctgacc agtcgttcgt tctcgtagcg tagcgagacc gccaggccgt ctatcttcag
     4801 ctccgtcacg tagcgcgggg tctcgtcttc gccgagcagg cgccgcacgc gggcgtccca
     4861 ctctctcagg tcttctatct tgcgggcgtt cgcaagggag agcatgggga cggagtgcct
     4921 gacttcctcg aacccctcga tgggctcgcc gccgacgcgc tgggtgggtg agtcaggggt
     4981 gatgagctcg ggatgctcgc cttccagggc ttccagctcc ctgtagatgg cgtcgtactc
     5041 ggcgtcggag atctccggcg cgtcatcgac gtggtagcgg cggttgtggt aacggacctg
     5101 ctcgcgcaga tcctctaccc taccccgaac gtccacgcct ccggccacgc aggaattata
     5161 cgcacctacg cacccgctcc caagagaagc ttgatcgcca gcacgccgta cagggcctga
     5221 gtttgcgcct gcatggagcg atccgcaacc ttttgctcca cagactgtta ccgaaacgtt
     5281 ataggactgt ggtcgaaagt ttacttatcc gtagccgtgc cgttacccac cttctttacg
     5341 atcaataccc actgccgacc gaggggaccg actagaaagg ggacacggag atgaggcgga
     5401 cggcgacgat a
//
LOCUS       DATMJA010000183         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_142631, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000183 DATMJA010000000
VERSION     DATMJA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142631"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VNT12_04350"
     CDS             <1..370
                     /locus_tag="VNT12_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWI45383.1"
                     /translation="ILPGMGGTQRLPRLIGPALAKELIFTGRRISAGEAREIGLVNSV
                     VAEGEALNAARELAAEISANGPLAVRHAKAAANRTLDVDLVSGLEFEADQFALLFATE
                     DAREGMNAFGEKRKPVFEGR"
     gene            367..765
                     /locus_tag="VNT12_04355"
     CDS             367..765
                     /locus_tag="VNT12_04355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWI45384.1"
                     /translation="MVHETKIRVRYAEIDAQGHVNNATYLSYFEVGRVEWLRATGHSY
                     REMERQGRGLVVVEALLQYSRPAFFDDELTLITNLAELGKVSLRFDYEVLRDGEVLVS
                     GHTRHACIDLATGKPVRMPQQLLRLAPGKL"
     gene            818..1591
                     /locus_tag="VNT12_04360"
     CDS             818..1591
                     /locus_tag="VNT12_04360"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HWI45385.1"
                     /translation="MLNSGSVNPLLGQHAYRLSEAGVAGTRTSHGAENNLYKIELLLG
                     HDESNTFGMGNLLRDVGFEEAYEAVALQIGYPPDREENPGRGCIDPARTAAGLVETGE
                     RIGAVVGSGGRLIFATGHPGAMILYYLGLSRWAKELGGDVSTVDTRGRYERGVSLDWA
                     ESVATLGDGASLFHTHDSEPMRDVLRQTGAVDLVVADHGFAGAAIAASVPTVVVMDTN
                     DPAFAVVAGRGADVTVVPMDDNRPLNVYSTALDVVRRGA"
     gene            1738..>2318
                     /locus_tag="VNT12_04365"
     CDS             1738..>2318
                     /locus_tag="VNT12_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI45386.1"
                     /translation="MSRAEVFDRNRPLLFSIAYRMTGSIMEAEDAVQEAYLRWQRASE
                     DEVRSPSAYLSKVVTRLCIDRLRSARMRREQYVGPWLPEPVLEEQAQEIGGQAEVEDS
                     LSMAFLVLLESLTPVERAVFLLREVFDYEYAEIASLVGKGEANCRQISRRARQSLAAG
                     RPRFESSPQQEERLMEGFLQASIGGDREALLELL"
BASE COUNT          457 a          728 c          746 g          387 t
ORIGIN      
        1 gatactccct ggcatgggcg ggacccagcg cctgccgcga ctgatcgggc ccgctctggc
       61 caaagagttg atctttaccg gccgccgcat aagcgccggg gaggccaggg agataggcct
      121 cgtcaacagc gtcgtcgccg agggcgaagc gttgaacgcg gctagggaac tggccgccga
      181 gatctccgcc aacggacccc tcgccgtaag gcacgcgaag gcggccgcga acaggacgct
      241 cgacgtcgat ctcgtaagcg gcctcgagtt cgaggctgac cagttcgccc tgctcttcgc
      301 caccgaggat gcccgcgaag gcatgaacgc cttcggcgag aagcgcaaac cagtgttcga
      361 gggaagatag tccacgagac aaaaatccgg gtccgctacg ccgagatcga cgcccagggg
      421 cacgtcaaca acgccaccta cctctcctac ttcgaggtag gacgcgtgga gtggctacgc
      481 gccacgggcc actcctacag ggagatggag cgccagggcc gcggcctcgt cgtcgtcgaa
      541 gcactcctcc aatacagcag accagccttc ttcgacgatg agctgaccct gatcaccaac
      601 ctagccgaac tcggtaaggt ctccctccgc ttcgattacg aggttctccg tgacggagaa
      661 gtcctcgtct caggccacac gcgccacgcc tgcatcgacc tggccacagg caaaccagtc
      721 aggatgccac agcaactgct caggcttgca ccaggcaagt tgtgagcatg tcagttagat
      781 cgtgcacacg ctaaagtcga aatgctgact agctgaaatg ctaaactcag gctccgtgaa
      841 ccctctgctc ggacagcacg cataccggct ctccgaagcc ggggtagcag gcacccgtac
      901 ctcccacggt gccgagaaca acctttacaa gatagagctg ctcctaggac acgatgaatc
      961 caacacgttc gggatgggga acctgctgcg ggacgtgggc ttcgaagagg cctacgaggc
     1021 cgtggccctc cagatcggct acccgccgga cagggaggag aatccgggac gcgggtgcat
     1081 agacccggcg cgcacggccg cagggcttgt cgaaacggga gagcggatag gggctgtcgt
     1141 cgggtcgggg gggcgattga tcttcgccac cgggcatcct ggagcgatga tcctctacta
     1201 cctcggactc tccaggtggg cgaaagaact gggcggagac gtttcaaccg tcgatacgcg
     1261 cggacgctat gagcgcggag tatccctgga ctgggcggag tctgtggcca ctctcggcga
     1321 cggggcaagc ctgttccaca cccacgactc tgagcccatg agggatgtac tgcggcagac
     1381 aggagcggtc gacctagtgg tcgccgacca cggtttcgcg ggggctgcga tcgccgccag
     1441 cgtccccacg gtcgtggtga tggacaccaa cgatccagcc ttcgccgtgg tcgccgggcg
     1501 aggcgccgac gtgacggtcg tgcccatgga tgacaaccgt ccgttgaacg tctactcgac
     1561 ggccctcgac gtggtccgaa ggggtgcttg atcacccacc cgtaagacca tcaccctctt
     1621 gcgttctaat gtcacgctgg accgggccgt ttcgtctttt tcgtagagcg acgtgatgag
     1681 gtgtacgcac agaagcaatt ttcggaagtc gtctcgcgaa ggcgaggtcg ggcagtggtg
     1741 agccgcgccg aggtcttcga ccgtaataga cccttgttgt tctcgatcgc ctacaggatg
     1801 acgggcagca tcatggaggc cgaggacgcg gtgcaggagg cctacctgcg ctggcagcga
     1861 gcctctgaag acgaagtgcg ctctccctca gcctacctct cgaaggtggt tacccgcctt
     1921 tgcatcgaca ggctacgctc cgcaaggatg cgccgcgagc agtacgtcgg cccgtggctg
     1981 cccgagccgg tgctagaaga gcaggcccag gagatcggag gccaggccga ggtcgaagac
     2041 tcgctctcga tggctttcct ggtgttgctc gagagcctca ccccggtcga gcgggccgtg
     2101 ttcttgctca gggaggtctt cgactacgag tatgcggaga tagcctctct cgtcggaaag
     2161 ggcgaggcca actgccgcca gatctcacgc cgcgccaggc aatcgctggc ggccgggcga
     2221 ccccgcttcg aaagctcccc gcagcaggag gaacgcctga tggagggctt cctgcaggca
     2281 agcatcggag gcgacaggga ggccctgctc gagctgct
//
LOCUS       DATMJA010000184         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_201651, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000184 DATMJA010000000
VERSION     DATMJA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201651"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VNT12_04370"
     CDS             <1..252
                     /locus_tag="VNT12_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWI45387.1"
                     /translation="LESALEQAKAVAGGKDVGVVGASLVQQCIRAGVLDEIHVDLVPI
                     LLGDGVRLLEHLGTELIELESTRVIEGSRVTHLTFRVVK"
     gene            complement(397..591)
                     /locus_tag="VNT12_04375"
     CDS             complement(397..591)
                     /locus_tag="VNT12_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45388.1"
                     /translation="MDNGFPEVLHVDLDDIRHLEMRYSRARPNKIELAVEYVGGRIRV
                     YEVDRFLIERLLEQLQYWDA"
     gene            complement(603..2546)
                     /locus_tag="VNT12_04380"
     CDS             complement(603..2546)
                     /locus_tag="VNT12_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HWI45389.1"
                     /translation="MSGATAAPKTQFARLKLWLLEKRIEEVQGPEASEGEEQHPWWQV
                     VCLTGVDYFSTLGYIPAIAAVAAGALSPIATLLIVLLTLFGMLPMYRVVSQQSPHGQG
                     SIAMLENLLSFWKGKLFVLCLLGFVATAWIVTITLSAADAAVHIVENPLVPGFLHGQG
                     VGITLLLLGVLGAVFLKGFREAIGIAVFIVGAYLLLNLVVVAVGFYEIATQPQTVADW
                     RSSLFADYGNPLVMIGVSLLVFPRLALGLSGFETGVSMMPLVRGNPEDDPVRPEGRIR
                     NTRKMLTVAALIMSFYLITTSFVTTLLIPAREFEEGGSASGRALAYLAHNHLGEAFGT
                     AYDLSTIAILWFAGASAMAGLLNIVPRYLPRYGMAPEWGRAVRPLVLVYTAISFVVTI
                     AFGAGVDAQAGAYATGVLAMMTSAAFAVTLTTWRGGSRRGGFAFGVVTAVFVYALVAN
                     AVQRPDGLMIAFFFGVAIVVVSLVSRVQRSTELRQERIEVDDAAQRFIDEAEEHIHLV
                     AHRRRQGTEEEYTRKEREQREDNHIPASEQILFLEVDVVDASEFEDVLEVRGVEVGGH
                     QVLRAESSVVPNAIAAFLLHLRDTTDKTPHCYFGWTEGNPIVYLFRYILLGEGDTAPV
                     THEVLREAEPDPEKRPAVHVGGR"
     gene            complement(2564..2839)
                     /locus_tag="VNT12_04385"
     CDS             complement(2564..2839)
                     /locus_tag="VNT12_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45390.1"
                     /translation="MPEQRPFLERIMFGRQHGPRAQREEKVLHYIIHRVDEDADLHDV
                     LQEPYVRRNCSQVEIDEIIMNPELVHHCREHLEHVFASGELDPKRQR"
     regulatory      complement(2846..3010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          598 a         1030 c         1042 g          594 t
ORIGIN      
        1 ctcgaaagcg ccctggagca agcgaaagcg gtcgcaggcg gcaaggacgt cggcgtggtc
       61 ggcgcgagcc tcgtgcagca gtgcatcagg gcgggagtcc tcgacgagat acacgtcgac
      121 ctggtgccca tcctgctcgg ggacggcgtc cggttgttag agcacctggg caccgagctg
      181 atcgagctag agagcacccg ggtgatcgag ggatctaggg tcacgcatct tactttccgc
      241 gtcgtaaagt gaggtaccat ccggagtcgc tggccgctgg gccgggagct aaggctccag
      301 gatctccctc ttcgtgccct tattcaccgt agtgcgtgga acgtaaattc tgcgaacttc
      361 gccgtgacgg agttctcgga agttcgctct ggaagatcat gcatcccaat actgcagctg
      421 ttcgagcaga cgctctatca ggaacctgtc aacttcgtat accctgatcc gaccgcccac
      481 gtactccacg gcaagctcta tcttgttggg tcttgcgcgg ctgtacctca tctccagatg
      541 gcgaatatcg tccaggtcca cgtgcagcac ttcggggaag ccgttatcca tgcgcatacc
      601 tctcaccgac cgcccacatg gaccgctggc cgcttctctg ggtccggctc ggcctcccgc
      661 aacacctcgt gggtgaccgg agctgtgtcg ccctcgccca ataggatata gcggaacagg
      721 tagacgatcg ggttgccctc tgtccagccg aagtagcagt gcggcgtttt gtccgtcgtg
      781 tcgcgcaggt gtagcaggaa tgctgcgatc gcgttgggga cgaccgaact ctcggcccta
      841 agcacctggt gcccgccaac ctccacgccc ctgacctcaa gcacgtcctc gaactccgat
      901 gcatccacga cgtcgacctc gaggaagagg atctgctcgc tggccgggat gtggttgtcc
      961 tcgcgctgct ctctctcttt gcgggtgtat tcctcttccg tgccctggcg ccggcggtgg
     1021 gcaacgaggt ggatgtgctc ttccgcttcg tcgatgaacc tctgcgccgc atcgtccacc
     1081 tctatgcgct cctgtcgcag ctccgtggag cgctgaaccc gcgacaccag cgagacgacg
     1141 acgatggcaa caccgaagaa gaaggcgatc atgaggccgt cggggcgctg tacggcgtta
     1201 gcgaccaggg cgtagacgaa caccgcggtg accactccga acgcgaaccc gccccgcctc
     1261 gagcccccgc gccaggtggt cagggtcacg gcgaaggcgg ccgaggtcat catggcgagc
     1321 accccggtcg cgtaggctcc ggcctgagcg tccacgcctg ccccgaaggc tatcgtgacg
     1381 acgaaggaga tggcggtgta gacgagcacg agcggcctga ccgcccgccc ccactccggg
     1441 gccatcccgt agcgcggcag gtagcgggga acgatgttca ggagcccggc catagccgag
     1501 gctcccgcga accacaggat ggctatggtg gagaggtcgt aggctgtacc gaaggcttcg
     1561 ccgaggtggt tgtgggcgag gtaggcgagc gcccgcccgc tggcgcttcc gccctcctcg
     1621 aactccctgg ccgggatgag cagggtggta acgaagctgg tggtgatgag atagaagctc
     1681 attatcaggg ccgcaacggt cagcatcttg cgcgtgttgc ggatgcgccc ttctgggcgc
     1741 acgggatcgt cttccgggtt gccgcgtacg agcggcatca tactcacccc agtctcgaag
     1801 cctgagagcc ccaaggccag ccgcgggaac acgagcagtg acaccccgat catgaccagc
     1861 gggttgccgt agtcggcgaa tagggagctc cgccagtcgg cgacggtctg gggttgcgtc
     1921 gcgatctcgt agaagccgac ggccacgacc acgaggttca gcagcaggta agccccgacg
     1981 atgaagaccg cgatgccgat cgcctcccga aagcccttca gaaagacggc gcccagcacc
     2041 cccagcagca ggagggttat gccgacccct tggccgtgca ggaagcctgg cacgagcggg
     2101 ttctcaacga tgtgcacggc ggcgtccgcc gcggagaggg tgatcgtgac tatccaggct
     2161 gtggccacga accccagcag gcagagcacg aacagcttgc ccttccagaa cgagagcagg
     2221 ttctcgagca tcgcgatcga gccttgcccg tgggggctct gctgggagac cacgcggtac
     2281 atcggtagca tcccgaagag ggtcaagagc actatgagaa gggtggcgat aggcgagagc
     2341 gctccggccg cgaccgccgc gatggcaggt atgtagccca aggtggagaa gtaatcgacg
     2401 ccagtcaggc acaccacctg ccaccacggg tgctgctcct cgccttcgct ggcctcgggc
     2461 ccctgtacct cctcgatgcg cttctcgagc agccagagct ttagccgggc aaactgggtc
     2521 ttcggtgcgg ccgtggcccc ggacatcgcc tatcctccga tccctagcgc tgccgcttcg
     2581 gatctaactc cccggaggcg aacacgtgct ccaggtgctc cctgcaatgg tggacgagct
     2641 cgggattcat gatgatctcg tcgatctcga cttgggagca gttgcggcgc acgtagggct
     2701 cctggagtac gtcgtgaagg tcggcatcct cgtccacgcg gtggatgatg tagtggagga
     2761 ccttctcctc gcgctgagcg cgagggccgt gctgcctgcc gaacatgatt cgttccaaga
     2821 agggtcgctg ttcgggcacg cttactacct ccttcttctg cgcctgcggg gttagctgtc
     2881 gggttcgggc cgaaagagtt aagccctacg ctggctggct tcgccggcct agcgattcac
     2941 cccgcggacc ttgggtccgt gtgggtcccc cgccccccgg cgccacccgg gagttaggcg
     3001 tcgtggctat gttgttgtca ctcatcgata tacgcccaga agggcgcact gtcaacaccc
     3061 atgagtcgag cttattcacc ggagtgcgtg gagaaactgt gtggaaattc cggagagggc
     3121 ccaaacaaga gctccgcggg cggcgcgagg gaccgaaagg cgccatttat gtcgcattgc
     3181 ccctccaaag actcacgatt cagacgcctc aagctatttt ccaaacagtt tcggaagggg
     3241 aattctccga agttgagcag ctga
//
LOCUS       DATMJA010000185         3110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_438681, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000185 DATMJA010000000
VERSION     DATMJA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438681"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VNT12_04390"
     CDS             complement(<1..332)
                     /locus_tag="VNT12_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45391.1"
                     /translation="MQSRFRTGMTLAMFSLVVFTIVTMSFITAAFGSIFDDTDRLSGG
                     FDVRADAGYAAPIPDMNKALKDKKSIDESDYTAVGTVTGLSVDAKQRGTDHKPQTLFL
                     QGVDGGYT"
     gene            complement(305..805)
                     /locus_tag="VNT12_04395"
     CDS             complement(305..805)
                     /locus_tag="VNT12_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45392.1"
                     /translation="MLATMLISASFTTGDTLTNSLRLQSLENLGQVDVQVQADGAGAS
                     GQQQFGDTSSERAGYFDAKVADEVRDRLSDENHVSGVAPAAIETVPVTAKGSDLSEPS
                     TDVLGLDQEQLEGFDRLTTSSGKKLDPTDLGKNQIYLSADAAKGLDVENGDVIPTRCR
                     AASVPA"
     gene            1168..1953
                     /locus_tag="VNT12_04400"
     CDS             1168..1953
                     /locus_tag="VNT12_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HWI45393.1"
                     /translation="MLRTVTATRYVTPLREGGSLPAIVEADDLGTYVLKFRGAGQGRK
                     ALVAELVAGEIARRLDLLVPEVVLADLAPELGRSEPDPEIQDLLKRSTGLNLALDYLP
                     GSLGFDPLVEPPDSGLASRIVWFDALVTNVDRTPRNTNLLVWHNRLWLIDHGASLYFH
                     HNWPARDGKALEAASRRPFAAVRDHILLPFAGSVFEADAALTPRLAPEVLRAIVGMIP
                     EEWLADEPGFGSIEEVRSAYVAYLSTRLQEPRAWVSALEETRR"
     gene            1950..2333
                     /locus_tag="VNT12_04405"
     CDS             1950..2333
                     /locus_tag="VNT12_04405"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HWI45394.1"
                     /translation="MSLFEYALLRVVPRVERGEFINAGVVLYCQDAKFLDARIRLDPD
                     RLQALDPNLDPETVQAHLEVARRVCEGGAEAGAVGLLPQGQRFGWLVAPRSTVVQPSP
                     VHTGFADDPEQAIEHLLRVMVDTPE"
     gene            complement(2466..2771)
                     /locus_tag="VNT12_04410"
     CDS             complement(2466..2771)
                     /locus_tag="VNT12_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45395.1"
                     /translation="MLDSRESSPPVAVLDPVSVTRSRDVTELLIGVLRSIEPYEACIL
                     EAEQDGDLEVAAFLRELRRQDLIRAREATRLLKRSLDAAYVNVGKVGEPYVELSTGS"
     gene            complement(2909..>3110)
                     /locus_tag="VNT12_04415"
     CDS             complement(2909..>3110)
                     /locus_tag="VNT12_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWI45396.1"
                     /translation="VAWLVILIAGWTVTTLLMLVLIGFLLVPVMALITLVPFVHSAYA
                     AYRVSKDGGYRYPLIADMIEAR"
BASE COUNT          561 a          985 c          973 g          591 t
ORIGIN      
        1 ttcgtgtagc cgccgtccac gccctgcaag aagagggtct gcggcttgtg gtccgttccc
       61 ctctgcttgg cgtccacgga gagcccggtg acggtgccga ccgcggtgta gtcgctttcg
      121 tctatgcttt tcttgtcttt cagggctttg ttcatgtctg ggatcggggc ggcgtaaccg
      181 gcgtcggcgc gcacgtcgaa gccgccggag aggcggtccg tgtcgtcgaa gatggagccg
      241 aaggccgccg taatgaagct catggtgacg atggtaaaga ccacgaggga gaacatggcg
      301 agggtcatgc cggtacggaa gcggctctgc atcgggtagg aatcacatct ccgttctcaa
      361 cgtcgagacc tttcgcagca tcagcgctga gatagatctg gttcttgccg agatcggtcg
      421 ggtcgagctt cttgcccgag gaggtcgtaa gcctatcgaa gccctccaat tgctcttggt
      481 ctaggccaag cacatccgtg ctcggttcgc taaggtcgct gcccttggct gttacaggga
      541 cggtctcgat ggcggcggga gctacgcccg agacgtggtt ctcgtcagaa agccggtcgc
      601 gcacctcgtc tgccaccttc gcatcgaagt agccggcacg ttctgaggat gtgtccccga
      661 actgttgctg gcctgaggca cctgcgccgt cggcctgcac ctgaacgtcc acctggccca
      721 gattctccaa ggactgtagg cggagggagt tggtcagggt atctcccgtg gtgaaagaag
      781 cggaaatgag catggtcgcg agcatcagac cgaggtctgc ggacggaacg cctccacgtc
      841 cagaccatcc cggtcgccct ggcctttcga gtatcgccct ccggcaaaca ttcccctccc
      901 ctgacgcccc ggaagggacg ccgcatcgtg ataggaattc gccagaaacg aacaaagggt
      961 cgccggggag gatggggaga acggcgaccc tcggtaactg attgtaccgg cagcggcgac
     1021 gatcccctac cccgaaggta gcgctagagc ccgcggcgcc ggtcgtgacc cgtctacggg
     1081 atgccgctct gtcgagcgta gggtgcgaga gccggtagaa tgggtggcgt cggttctccc
     1141 ggtaacaccc aagccgaggt aatcagcttg ctaaggacgg tcactgcgac gaggtacgta
     1201 acgcctttgc gtgagggggg ttcgctgccg gcgatcgtcg aggctgacga cctcgggacc
     1261 tacgtcctca agttccgcgg ggctggacag ggccgcaagg cactggtggc ggagttggtg
     1321 gccggtgaga tcgctcgccg cctggatctc ctcgtgcctg aggtcgtgct tgccgacctt
     1381 gcgcccgagt tgggccgctc tgagcccgac cccgagatac aggacctgct gaaaaggagc
     1441 acaggcctga acctggcgct ggactacttg ccaggctctc ttggcttcga cccactcgta
     1501 gagccgccgg attccggcct cgcctcgcgc atcgtctggt tcgatgccct ggtgaccaac
     1561 gtcgaccgca ccccgcgcaa cacgaacctc ctggtctggc acaaccgtct ctggctcata
     1621 gaccacgggg cttcgctgta cttccaccac aactggccag caagagacgg gaaggccctg
     1681 gaggccgcga gccggcgccc cttcgccgcc gtcagggacc acattctgct ccctttcgca
     1741 ggctctgtct tcgaagccga cgcggcgctc accccgcggc tcgccccaga ggtgctccgc
     1801 gccatcgtag gcatgatccc cgaggagtgg ctcgctgacg agccaggctt cgggagcatc
     1861 gaagaggtac gctccgctta cgtcgcctat ctgagtaccc gtctgcaaga gccgagagcg
     1921 tgggtgagtg cactggagga gaccaggcga tgagcctttt cgagtacgca ctcctgcgcg
     1981 tggtgccgcg cgtcgagcgc ggtgaattca taaacgcggg ggtggtgctc tactgccagg
     2041 acgcgaagtt cctcgacgcg cgcatccgcc tcgacccgga ccgtctgcag gcactcgacc
     2101 ccaacctcga ccccgagacc gtacaggcac acctggaagt cgcgcgccgg gtgtgcgaag
     2161 gcggagctga ggctggcgca gtgggattgc tgcctcaggg ccagcgcttc ggatggctcg
     2221 tcgcgccccg cagcaccgtg gtgcaacctt ctccggtcca tacgggcttc gccgatgacc
     2281 ccgaacaggc catagagcat ctcctgaggg ttatggtcga tacgcccgag tagatgccca
     2341 gtcgtggtcg tcagcgacct ggcacccttt ggcgttcttc acccttgtgc gcatgcggac
     2401 cgagacgagc ctgaaacgtg tttgcctgta ggccggtttg cagatggcct gctctgcaaa
     2461 cccctctaag atccagtgga gagctcgaca tacggctctc ccacctttcc gacgtttacg
     2521 taagccgcgt cgagagatct ctttagtagg cgggtcgctt ccctggcccg aatgaggtcc
     2581 tgacgccgca actcgcgcag gaaggcggcg acctcgaggt ccccgtcctg ttcggcctcg
     2641 aggatacacg cctcgtaggg ctcgatactc ctcaatacgc cgatcaggag ttctgtcacg
     2701 tcacgacttc gcgtaaccga cacgggatca aggactgcca ccggtggaga actttccctg
     2761 ctgtccagca tctctatcct ccttgcgaat ccacgtttct tacgtatacg cacgacgtgt
     2821 acgaatggtg ccagaaccgg aagaaaatcc tcctggggcg atgcccccaa cccatacggg
     2881 tcgggggcgc cgtctctctg agtgccttct aacgggcttc gatcatgtcg gcgatcaatg
     2941 gatagcggta cccgccgtcc ttgctaaccc ggtaggccgc gtaggcgctg tgcacgaatg
     3001 gcaccagtgt gatcagagcc attacaggga cgagtaggaa cccgatcaag accagcatca
     3061 agagcgtcgt cacggtccac cccgcgatca ggatcacaag ccaagccacc
//
LOCUS       DATMJA010000186         2983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_320638, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000186 DATMJA010000000
VERSION     DATMJA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320638"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VNT12_04420"
     CDS             complement(<1..189)
                     /locus_tag="VNT12_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45397.1"
                     /translation="MDCKDRLEAYLREKQVPLEIRHHPRAITAQEVAASEHVPGKMLA
                     KTVMVLADGRMVMLALPAP"
     gene            716..1084
                     /locus_tag="VNT12_04425"
     CDS             716..1084
                     /locus_tag="VNT12_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45398.1"
                     /translation="MARLYCRIVGPVLLVVGVLGVVQFGIPGFLSINEPVEIGLHFLT
                     GALATYAGFSRGYGRAAVLYARVFGIVYLILCIGFFVPDVLPGLIHFDLGCNLVHLVL
                     ATWGIYAGYFARETTTAPAT"
     gene            1422..1913
                     /locus_tag="VNT12_04430"
     CDS             1422..1913
                     /locus_tag="VNT12_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI45399.1"
                     /translation="MMIRQMEMSDVDDLMGIFSDPVAMRYYPNTKSRQEAEEWVRRMQ
                     ESYRDHGFGLWVAVLEDSGEFVGQCGLTVQEVEGKNEVEIGYLFLRRFWGRGLATEAA
                     RAARDHGFHMPGCERLVSLIDPGNLASRRVAEKVGLRPERGVWKWNKEICVYAIHKGN
                     VAT"
     gene            complement(1954..>2983)
                     /locus_tag="VNT12_04435"
     CDS             complement(1954..>2983)
                     /locus_tag="VNT12_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWI45400.1"
                     /translation="TSGVKDLRAALDGARERLKYEGRGTLVFVDEVHRFNKNQQDALL
                     PAVEEGLVDFIGATTENPSFEVTAPLLSRSRVLRLRSLTEEDLGLLLDRSVEELGIDV
                     SDEAREYLLRLAGGDGRRMLNAVEVASAGTKRVEVADVERAVGQRALRYGREEHYDVI
                     SAFIKSVRGGDPDAALHYLARMLEAGEDPVFVARRLVILASEDIGNADAHGLPLAVSA
                     AEAVRLIGMPEGRIPLAQATTYLASAPKSNAAYTGINAALADVRQENTPEVPMHLRNA
                     VTGLMKEEGYGLGYRYAHDDEPEGMNDTYLPEELVGRVYYEPKKSGDEAEIKARLQRW
                     REERERRT"
BASE COUNT          536 a          934 c          937 g          576 t
ORIGIN      
        1 gggagccggc agcgcaagca taaccatcct gccgtcggcc aagaccatca ccgtcttggc
       61 cagcatctta ccagggacgt gctcgctggc ggcaacctcc tgggcggtga ttgccctagg
      121 gtgatgtcga atttctaaag gaacctgctt ctctctgagg taggcttcca gcctgtcctt
      181 acaatccacc gcatgacctc ctttgtcccg agcatggggc catgtcgacc gctgctccac
      241 ctagtcacaa tgataccaaa gttgatcgct gggcgatttg accgccttcg ggggaagctc
      301 gcgggctgag cgggggaggc gctggcgggg gcgaactact accgccttct agtggaccca
      361 cgcaagttag gacaacggcc atctgggcgt tccgcgcggt agtaaggccg gacagcgagg
      421 tggagatggt gatcgtactt ctgccaccta ctcctcggcc agtgctgcga cgagttcgcg
      481 gcagaaatcc ggatgtctgc aaccacagag gaagcgtggg tccactatgc caccctctac
      541 ttcatgcagc agatcgacgc cgtgccgcag cagtcgaaga gtgatccccg cagctgcctg
      601 cctcgtagtt aagtgcaaga cggtgcaatc tagtgcctgg aagaggtcga acgtctggag
      661 gcgtgcgcag aacttgccag gttctaccaa gtggaagaag ggaggtgaat tgtatatggc
      721 cagactttac tgccggatcg taggtcccgt gctgctggtg gttggggtcc taggggtggt
      781 ccagttcgga atccccggct ttctgagcat caacgagccc gtcgagattg ggctgcactt
      841 cctcacgggc gccctcgcta cctacgctgg ctttagccgt ggctacgggc gagcggcggt
      901 gctctacgcc agggtgttcg ggatcgtcta cctgatattg tgtataggct tcttcgtccc
      961 cgacgtgctc cccgggctca tccacttcga cctggggtgc aaccttgtgc acctggtcct
     1021 ggcaacgtgg ggcatctacg ctggctactt cgcgcgggag accacgaccg ctccagccac
     1081 ctagaggctc tggtgggacg ggatctacgc gtcccgtccc gcttccaatc caggaaggac
     1141 atctggtgtc ggaccagaac aaaacccctc acccagcgtt tcgtggaggc cttcaacaaa
     1201 ggagatcgag ggcgaacgcc tcgccgccga gaacgagcgt tccgctcggc tgcgctgggt
     1261 cttcgaggcc atcggcctcg ccgtgaccgc ccacaaagac gggacgctgc tgcggtggtc
     1321 gtgcggcaag cggactttgc cagccgtcag gggtttcgag agttccgtgc tcaggcggat
     1381 ctacgcaccc gataacgaat aaagttctcc agacggagcg gatgatgatc cgccagatgg
     1441 agatgagcga cgtcgacgac cttatgggca tcttctccga cccggtggcg atgcgctatt
     1501 acccgaacac caagagccgc caggaggcgg aggagtgggt tcgcaggatg caagagagct
     1561 atcgagatca cggcttcggc ctctgggtgg cagtcctcga agactcgggc gagttcgtgg
     1621 gacagtgcgg cctcaccgtg caggaggtag aaggcaaaaa cgaggtcgag atcggctacc
     1681 tcttcctgcg gaggttctgg ggtcgaggtc ttgcgacaga ggcggccagg gcggcaagag
     1741 atcacggctt ccacatgcca ggctgcgagc gcctggtctc cctaatagac cctggcaacc
     1801 tagcctcccg ccgtgtcgcc gaaaaggtcg gcctcaggcc ggagagggga gtctggaagt
     1861 ggaacaagga gatctgcgtc tacgcgatac acaagggcaa tgtggcgacg tagtgtgatc
     1921 ctgcagggta gagacctctc ggcgttcgag atcctaagtc cggcgctccc tctcctcgcg
     1981 ccagcgttgc agccgagcct tgatctccgc ctcatctccg ctcttcttcg gctcgtagta
     2041 gacgcgccct acaagctctt ctggcagata cgtgtcgttc atcccctcgg gctcgtcgtc
     2101 gtgggcgtag cggtaaccca agccgtagcc ctcctctttc atgagccccg tcacggcatt
     2161 gcggaggtgc atgggaacct ctggcgtgtt ttcctggcgc acgtccgcca gggccgcgtt
     2221 tatgccggtg taggctgcgt tggatttcgg cgctgaagcc agataggtcg tagcctgggc
     2281 gagcggtata cgtccctcag gcatcccaat gagcctgacg gcctcggcgg ccgagacggc
     2341 gagcgggaga ccgtgagcgt ctgcgttgcc gatgtcttct gaggccagga tcacgagccg
     2401 cctggcgacg aacacggggt cctcccccgc ctccagcatc ctggccaaat agtgcaacgc
     2461 cgcgtcaggg tcgccgccgc gcacgctctt gatgaacgcg ctgatgacgt cgtagtgctc
     2521 ctcacgcccg tacctgagcg ccctctgccc aaccgcccgc tccacgtccg cgacctcgac
     2581 ccgcttggtg ccggcggagg cgacctcgac ggcgttgagc atccgccgtc cgtctccgcc
     2641 ggcgagccgc aagaggtact cgcgcgcttc gtccgagaca tctatcccaa gctcctccac
     2701 gctcctgtcg agcaggaggc cgagatcctc ttccgtgaga gaccgcaacc tgagcacgcg
     2761 cgagcgggat agcagcgggg cggtcacctc gaaggacggg ttctccgtgg tcgcccctat
     2821 aaagtccacg agaccctctt cgacggcggg gagcagggcg tcctgctggt tcttgttgaa
     2881 ccgatggacc tcgtctacga acacgagcgt cccgcgtccc tcgtacttga gacgctcacg
     2941 ggcgccgtcc agtgcggccc tgagatcctt tacaccactc gtt
//
LOCUS       DATMJA010000187         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_794751, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000187 DATMJA010000000
VERSION     DATMJA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794751"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..983
                     /locus_tag="VNT12_04440"
     CDS             105..983
                     /locus_tag="VNT12_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWI45401.1"
                     /translation="MVRAEKAPLEGMIALPGGKFLMGTDDGEGFPRDGEGPVRRITLD
                     PFYISAFATTSAEFSTFVEETGYETEAERFGWSFVFYRFLSAKAKKKVRGAVESAPWW
                     RAVGGASWRHPEGPGSGIKIRMNHPVVHVSYNDAAAYCDWAGKRLPTEAEWEYAARGG
                     LEQRRYPWGDTLTPNQEHRCNIWQGEFPTRNTVKDGYAGTAPVDSFSPNGFGLYNVSG
                     NVWEWCLDWFSATFHVAGRRDNPTGPPAGKARVIRGGSYLCHKSYCNRYRVAARSSNT
                     PDSSTGNTGFRCAANA"
     gene            complement(1017..1208)
                     /locus_tag="VNT12_04445"
     CDS             complement(1017..1208)
                     /locus_tag="VNT12_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45402.1"
                     /translation="MGEEKDTTEYTIKQVGDRYYPVIVDREAGGHYEINNPLTGGVLS
                     YKNPEAAEKYIQRAREKEG"
     gene            complement(1217..1636)
                     /locus_tag="VNT12_04450"
     CDS             complement(1217..1636)
                     /locus_tag="VNT12_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45403.1"
                     /translation="MRDEMNLTHEYMHHRTGYRLGAGCCWIKIYKGAECDAPVVVCEE
                     LPRVGGVATREVSGYLAAEVIREHFSGGLPDLPRPMLWVEHRPARRRGPGKYFLHTFP
                     SYRPRTVGAGFVRRVTLGTSHRELLAPAEVAILIREV"
     gene            complement(1645..>2548)
                     /locus_tag="VNT12_04455"
     CDS             complement(1645..>2548)
                     /locus_tag="VNT12_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI45404.1"
                     /translation="NRGLSEPGLFPEVEKLEGERDGDLSALRGRRWDAVIDTCGYVPR
                     VVGASAGLLADAVDHYTFVSSISVYSDAIAPGADEEAPVRELPEPTVEEVTGETYGGL
                     KALCERAAEETMPGRVLNVRPGLISGPHDPTDRFTYWPRRIAAGGEVLAPDHEERKVQ
                     YIDVRDLASWIVEMSEQRRTGTYNATGPDYELRMGRLLEACEAVGGAGAEPVWVSEDF
                     LEEKGVEPFTELPLWVPRDYAALHAIDCGKAIEAGLTFRPLSETIEDVLDWNRATAAG
                     RELAAGLRPEREQELLRAWHGVTL"
BASE COUNT          454 a          831 c          796 g          467 t
ORIGIN      
        1 gtggagtaga gaaagcaggt cgaaagcagc agggcgggat cgcaccgtct tgttgcacgc
       61 ctgcgaggtt ctcttcggag gagcctgctg gaagaccgca gagtatggtg cgcgcggaga
      121 aggctcctct ggaaggcatg atcgcgcttc ccggcggcaa atttctgatg ggcactgacg
      181 acggcgaggg atttccgagg gacggcgagg gtccggtgcg tagaatcacg cttgaccctt
      241 tctacatctc agcgttcgcg accacgagcg ccgagttttc gacgttcgta gaggagacag
      301 gctacgaaac ggaggccgag aggttcggct ggtctttcgt cttctacagg ttcctctcgg
      361 ccaaggcgaa gaagaaagta aggggagcgg tcgaaagcgc cccctggtgg agggccgtcg
      421 gtggagcgag ctggaggcat cccgaggggc cgggctcggg tatcaagatc cgcatgaacc
      481 accccgttgt ccacgtctcc tacaacgacg ccgcggctta ctgcgactgg gcgggcaaga
      541 ggttgcctac cgaggccgag tgggagtatg cggcaagggg cggcctcgaa cagagacgct
      601 acccctgggg cgacacgctg accccgaacc aggagcaccg ctgcaacatc tggcaggggg
      661 agttcccaac caggaacact gtcaaagacg gctacgctgg cacggcaccc gtggattcgt
      721 tttcccccaa cggcttcgga ctctacaacg tctccggcaa cgtgtgggag tggtgcctgg
      781 actggttctc cgccaccttc cacgtcgccg gccggagaga taacccgacc ggaccacccg
      841 cggggaaggc cagagtcatc cgtggaggtt cctacctgtg ccacaagtcc tactgcaacc
      901 gctaccgggt ggcggcgcgc agctccaata ccccggacag ctctacaggc aacaccggct
      961 tccgctgcgc ggcaaacgct taagggcggt gtaggagcag ttcgcgaacc gccttactac
     1021 ccttctttct cgcgggcgcg ctgtatatat ttctctgcgg cttcggggtt cttgtagctc
     1081 aggacgcctc cggtaagagg gttgtttatc tcgtagtgac cgcctgcctc cctgtcgacg
     1141 atgaccggat agtagcggtc tccgacctgc tttatagtgt actcggtcgt gtccttctct
     1201 tcgcccaact tgtctcctat acctctcgaa tgagaatggc tacctctgcc ggagccagga
     1261 gttcgcgatg cgacgttccg agagtgacgc gccgcacgaa gcctgcgccc acggttctgg
     1321 gacggtagga ggggaaggtg tggaggaagt acttgcctgg cccgcgcctg cgggcggggc
     1381 ggtgttcgac ccagagcatt ggacggggta gatccggcag accaccagag aagtgctccc
     1441 tgatcacctc ggcggccaga taccctgaca cctccctggt ggcgacgccg ccgaccctgg
     1501 ggagctcctc gcacacgaca acgggagcgt cgcattcggc gcctttgtag atcttgatcc
     1561 agcagcaccc ggccccgagc ctgtagcctg tcctgtggtg catgtactcg tgcgtgaggt
     1621 tcatctcatc tcgcaacccg cattctacag ggtgacaccg tgccaggccc ggagcaactc
     1681 ctgctccctc tcgggcctga ggccggcagc aagctctcta cccgccgccg tggcgcggtt
     1741 ccagtccagc acgtcctcga tcgtctcgga caagggccgg aaggtgagcc ccgcctcgat
     1801 agctttgccg caatcgatgg catgcaatgc ggcgtagtcc cgcggcaccc acaatggcag
     1861 ctccgtgaac ggctcgactc ccttctcctc caggaagtcc tcggataccc agacaggctc
     1921 cgcgccggca ccgccgacag cttcgcacgc ttccagcaat cggcccatcc gtagctcgta
     1981 gtcggggccc gtagcgttat acgttcctgt ccgccgctgc tcggacatct ctacgatcca
     2041 cgacgcgagg tctcgcacgt cgatgtattg caccttacgc tcttcgtggt caggggccag
     2101 cacttcgcct ccggcggcga tccgacgcgg ccagtacgta aaacggtccg tggggtcgtg
     2161 cggaccagag atcaaaccag gacggacgtt caacaccctg cctggcatcg tctcctcggc
     2221 ggcccgctcg cacagcgctt tgagacctcc gtaagtctcc cccgtgacct cctcgaccgt
     2281 cggttcgggg agctcccgca ccggcgcctc ttcgtcggcc cctggcgcga tggcgtcaga
     2341 gtaaacggag atgctggaga cgaaggtgta atggtcgacc gcatcagcaa gtagcccggc
     2401 agacgccccc acgacgcggg gcacgtagcc gcaggtgtcg attaccgcgt cccagcgccg
     2461 cccccgaagg gctgagaggt ccccgtccct ctcgccctcg agcttttcga cctcggggaa
     2521 cagccctggc tcgctgagcc cccggttg
//
LOCUS       DATMJA010000188         3666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_143476, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000188 DATMJA010000000
VERSION     DATMJA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143476"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..390)
                     /locus_tag="VNT12_04460"
     CDS             complement(19..390)
                     /locus_tag="VNT12_04460"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWI45405.1"
                     /translation="MRKTVMAEKGPAPAGPYSHAVVAGDFVFISGQGPVDPETGTMPD
                     AFTDQVRQTFENVRTILDAAGSSLDNVVKVNAYVTDLTRFAEFNEVYKEYFENDPPAR
                     TTVGADLLGMLVEVDCIAVVE"
     gene            complement(402..599)
                     /locus_tag="VNT12_04465"
     CDS             complement(402..599)
                     /locus_tag="VNT12_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45406.1"
                     /translation="MLDLVLENRRLVDETGNPWFVGDVGIEDGTIVEVGRVGRSSHAL
                     VNGTKAVESGALMETEKGEVI"
     gene            complement(609..1655)
                     /locus_tag="VNT12_04470"
     CDS             complement(609..1655)
                     /locus_tag="VNT12_04470"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWI45407.1"
                     /translation="MERNIQKMAHMARENGVALRPHVKTHKIPDIAREQLEAGASGIT
                     MAKLSEAEVMADGGIEDIFIAYPLVTEAKIRQAVRLAERVRLVVGVDSLEGARRLSGV
                     AEDQILEVRLEVDTGLRRTGVPLDEAVGLAGEIEAMDNLDLTGIYTYRGAVLGGSKTL
                     ELEKAGLEEGQLMVSLAVMLRERGIGVDDVSVGSTPTAEHVAKVEGVTEIRPGTYVFY
                     DRMQARLGACSLDECAAVVVCTVVSRPTRELAVIDGGSKTFATDVAPGAEPLNLEGYG
                     HVIGYPSAVLERLTEEHGMLSVDEECDLEVGDTLQIIPNHICSTVNLHDEVYLVREDG
                     VVEETRVAARGKVR"
     gene            complement(1830..2072)
                     /locus_tag="VNT12_04475"
     CDS             complement(1830..2072)
                     /locus_tag="VNT12_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWI45408.1"
                     /translation="MPTTSPCQTGGSSWRVVMDVGEWDDSLFINSPGQSGDPASPHYS
                     DLFGPWARDETVPLLYSRRKVEAVAERRIVLVPTGQ"
     gene            complement(2127..3545)
                     /locus_tag="VNT12_04480"
     CDS             complement(2127..3545)
                     /locus_tag="VNT12_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWI45409.1"
                     /translation="MSGAEICFLEATELARLIRGREASAEETMEACLDQVERTNPRVN
                     AIITLVPERAMREARAADEFLREALARGEDVRPLHGLPVAHKDLVPTRGIRTTFGSLI
                     YEDCVPEEDALIVERLRRAGAITVGKTNTAEFGAGSQTYNEVFGETLNPYDTTRTCGG
                     AAVALACGMVPLADGSDMGGSLRNPASFCNVVGFRPSLGRVPSWPTQAAWSSLSTEGP
                     MARTVRDVALMLGVISGPDPRSPLAISGPGEDFFGPLERDFSGTRVAWSRDLGGLPVD
                     PRVTAVLDEQRHVFEDIGCLVEDNEPDFTDADEVFKALRAWHFELNYGELLKEHRGRM
                     KNTVVWNIERGVGLSGPDRGRAERKRTELYHRVRTFMEGYDFLAAPVSQVPPFDVKRR
                     CVTEINGVEMETYIDWMKSCYYVTVTGLPAISVPCGFTPEGLPVGVQIVGRPRDDLGV
                     LQLAHAFEQVTGFGKHRPPIVA"
BASE COUNT          654 a         1255 c         1012 g          745 t
ORIGIN      
        1 tgatgctagc gactgtaact actcgaccac ggctatgcag tccacctcta ccagcatgcc
       61 tagcaggtct gctcctacgg tcgtgcgtgc cggtgggtcg ttctcgaagt actctttgta
      121 cacctcgttg aactcggcaa accgcgtgag gtcggtcacg taagcgttga ccttcacgac
      181 gttgtcgagg ctcgagccgg cggcatcgag gatcgttctt acgttctcga aggtctggcg
      241 tacctggtcc gtaaaggcat caggcatggt acctgtctcc gggtccacag gaccctgccc
      301 cgagatgaac acgaagtcgc cggcgaccac ggcgtgggag tatggtccgg cgggtgccgg
      361 ccccttctcg gccataacgg tctttctcat cggttctcct ttcatatcac ttcgcctttt
      421 tcagtctcca taagagcacc ggattctacg gctttcgtcc cgttgacgag ggcgtgagag
      481 ctccttccca cccttccgac ttccactatc gtaccgtctt cgatcccgac gtctccgacg
      541 aaccaggggt tccccgtctc gtccacaagc cgcctgttct ccagcacgag gtccaacacc
      601 gcagcctcct accgaacctt accgcgcgcc gccacccgcg tctcctccac gacaccatcc
      661 tccctgacca ggtatacctc atcatgcaga tttaccgtgc tgcaaatatg gttgggaatt
      721 atctgcaacg tgtcaccaac ttcgaggtcg cactcttcgt caaccgagag catcccgtgc
      781 tcctccgtga gcctctccag gactgcgctg gggtatccga taacgtgccc gtacccctcc
      841 aggttcaggg gttccgcgcc gggggctaca tcggtggcga aggtcttgct cccgccgtcg
      901 attactgcca gctccctggt aggcctactc accaccgtgc acaccaccac ggctgcgcat
      961 tcgtcgagcg aacaggcacc gagacgggcc tgcatcctat cgtagaacac gtaggttccg
     1021 ggtctgatct ccgtcacccc ctccaccttc gccacatgct ccgccgtcgg agtagagcca
     1081 acgctgacgt cgtccacccc tatgcctcgt tccctcagca tgaccgccag ggacaccatt
     1141 agttgcccct cttcgaggcc ggccttctcg agctccagcg tctttgaccc gcccagaacg
     1201 gcgccgcggt aagtgtagat tcccgtgaga tccaggttgt ccatggcctc gatctcacct
     1261 gccagcccga cggcttcatc aagaggaacc cctgtacgcc tcaggcccgt atccacctcg
     1321 agcctgacct ccagaatctg atcctccgca accccagaga gcctccttgc cccttcgagg
     1381 ctgtccaccc cgacgacgag cctaaccctc tcagccagcc gaaccgcctg ccggatcttc
     1441 gcttccgtca ccagcggata ggcgataaag atatcctcga tcccaccatc agccatgacc
     1501 tcagcctcag agagcttggc cattgtgatg cccgaagccc cggcctccaa ctgttccctc
     1561 gctatatcag gaatcttatg cgtcttcacg tggggtctca gcgcaacacc gttctccctg
     1621 gccatatggg ccatcttctg tatgtttctc tccatcttcg ggccgtcgac caaaaggtag
     1681 ggtgtcccag aagaatgcgg aatttggtat tgggaatcgg gaattgttcg cttactcggc
     1741 actatttccg acttcgaaat tcacacttca caattcaatg ctagcatctt tcgtcagttt
     1801 cctgcttcag cagactacga ggggcgcgct tactgtcctg ttggcacgag cacgatccgt
     1861 ctttccgcaa cggcctcgac ctttcgtctg ctgtacagca atggtactgt ctcgtctctg
     1921 gcccacggac cgaatagatc cgagtagtgc gggctcgcgg ggtcgccgga ttgccctggc
     1981 gagttgataa acagggagtc atcccactcc ccaacgtcca tcaccacgcg ccacgaggag
     2041 ccgccggtct ggcacggaga ggtagtcggc atggagcttc accgtgcgga tcggggagag
     2101 atccaacctt ctaccgtccc ctgggatcat gccacgatcg gcgggcgatg cttcccaaag
     2161 cccgtaacct gctcgaaggc atgggcgagc tgaaggacgc ccaggtcatc cctgggacgg
     2221 cccacgatct gaacccctac gggcaatccc tccggtgtga aaccgcaggg caccgagatg
     2281 gcgggcagcc ccgtaaccgt cacgtagtaa cagctcttca tccaatcgat gtaagtctcc
     2341 atctccaccc cgtttatctc cgttacacag cgccgcttca cgtcgaaggg cgggacctgg
     2401 ctcacgggcg ccgcgaggaa atcgtagcct tccatgaaag tccgcacgcg gtgatagagt
     2461 tcggtccgct tgcgctcggc gcgtcccctg tcagggccgc tcaagcctac gccccgctcg
     2521 atgttccaga ccacggtgtt cttcatgcgc ccccgatgct ctttgagtag ctccccatag
     2581 ttgagctcga agtgccaggc gcgcagagct ttgaagacct cgtcggcatc cgtgaagtct
     2641 ggctcgttgt cttccaccag acaacctata tcctcgaaga cgtgtcgctg ttcgtccagg
     2701 acggcagtca cccgcgggtc gaccggcaac ccgccgaggt cgcggctcca ggccacccgc
     2761 gtacccgaga agtctcgctc cagagggccg aaaaagtcct ctcccggccc agagatggcg
     2821 aggggcgagc gtgggtccgg gccggagatc acacctagca tcagggcgac gtcccgaacc
     2881 gtacgcgcca tcggaccctc cgtggagagc gaggaccagg ccgcctgtgt aggccaggag
     2941 ggcaccctgc cgagagatgg cctgaaacca acgacgttgc agaaggaggc gggattgcgc
     3001 agggagccgc ccatgtcgct cccatccgca aggggcacca tcccgcaggc gagggccact
     3061 gctgcgccgc cgcaggtcct ggtggtgtcg tacgggttga gggtctcgcc gaacacctcg
     3121 ttgtaggtct gggagcccgc gccgaactcc gcggtgttgg tcttgcctac cgttatggcc
     3181 ccggcccgtc gcaggcgctc gacgatgagg gcgtcctcct cagggacgca gtcctcgtag
     3241 atgagggagc cgaacgtggt acggatcccc ctggtcggta ccaggtcctt gtgcgccacc
     3301 ggcaagccat ggagcgggcg gacatcctcc ccacgcgcaa gcgcctcgcg gaggaactcg
     3361 tcggcggcgc gggcctcccg catggcccgc tcagggacga gcgtgatgat cgcgtttacg
     3421 cgcgggttgg tgcgctcgac ctgatccagg cacgcctcca tcgtctcttc ggccgacgct
     3481 tcccgcccgc ggatgaggcg ggccaactcc gtagcctcca gaaagcagat ctccgccccg
     3541 ctcacctacc ctatccccgg tctcctgacg atggcgggac gtatcccgcc gtaggttact
     3601 gtgccctttg gcttgggaat ttggaacttg gacctttgaa ttgttcgttc gacgcgcttt
     3661 cctact
//
LOCUS       DATMJA010000189         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_676476, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000189 DATMJA010000000
VERSION     DATMJA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676476"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..1596
                     /locus_tag="VNT12_04485"
     CDS             502..1596
                     /locus_tag="VNT12_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI45410.1"
                     /translation="MEDEERSFEEHPEAARILDERFWEIFEDYKCRLREMKSLLIVGD
                     GTTPEQLENNARSVLGRAAKVLGGEERSLLAVEEEIYRNIESSVEPLNPHPDESFRAG
                     VALCKATVTVVVKNLVPATPPEKIAEIALAVQEIVMDRVSRMVMASYVDYLLTKVKET
                     QFEERRRYSRELHDRLAHQMTVVTQSLDLYRTFRESDAERAERRLALAQESARESLDL
                     MHEFAKELRLLETSDGLKIALENLMRISVPEGVYYEVSLEGEEQHLPDYVRDQLYMVL
                     REGVRNAAAHSGSDRIEVEVGITPKHVTARVRDHGTGLNVEDGTTEGLGLSSMKERVS
                     LLGGSFGLVPEPGGGAKAEVSLPLMREKIR"
     gene            1629..2276
                     /locus_tag="VNT12_04490"
     CDS             1629..2276
                     /locus_tag="VNT12_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI45411.1"
                     /translation="MLLADDHALVRQGMAEMLSTDEGIEVVGEAADGREAVDLARKHG
                     PDAVVLDVEMPVMSGQVALRKLLDLRPPPKVVIVTVFADESHVRELLALGASAYLSKN
                     ASMRDLISTVRSVVEDREGQNEDDVILFVPRAAFDKDAPKASELSGREAEILMLAARA
                     RSNREIAERLHLSETTVKRHLSNVYEKLGVHTRAEAARKALSEGWISAWDVSRED"
BASE COUNT          461 a          690 c          782 g          377 t
ORIGIN      
        1 accggcgtgt gttgcaccgg tcccgcgtcc atcaggccgc tccaagaggg cccggcgccc
       61 aagggtaaga caaagggaaa ggaggaacgg gcgccggttc acgagtaaga ccctaccctg
      121 cttcaggcct ccgtgaacgc aaatccgggc gttctcggga aagtaggcac ggaaggtgca
      181 ggcttttggt ccgaatggtg taggggaatg caaaacccaa ttaggcgtgc ctcgaagcct
      241 ccgtttgcgt gcctcgcgcg acgcttcggg gcccgccgac ggctcaccaa cggcctccct
      301 taccgcgtct gcatgccgat tcccgtggtc ttcagaggtc cactgtggtc cgcgcccttt
      361 tagtcgcggt acgtggcggt caggaggtag gcgagcaatt ccgagtggct cggggctctg
      421 cagccgcatg gtattctgag aggcgaacaa gagggtggtg tgagccttta gacaaggatt
      481 ttggaagaag cggggagagc tttggaagac gaggagagaa gtttcgagga gcatccggag
      541 gcggcccgga tactcgacga gcgcttctgg gagatcttcg aagactacaa gtgccgtctg
      601 cgtgagatga aaagcctgct gatcgtgggc gacggcacca cgcccgagca gctcgagaac
      661 aacgcacgct cggtgttagg ccgggcggcg aaggttctcg gcggcgagga gaggagcctg
      721 ctcgccgtgg aggaggagat ctaccgcaac atcgagagtt ctgtcgagcc cctgaatcct
      781 caccccgacg agtccttcag agccggtgtc gctttgtgca aggcgaccgt cacggtcgtg
      841 gtgaagaacc tcgtgccagc gacgcccccg gagaagatcg ctgagatagc cttggctgtg
      901 caggagatcg tcatggaccg tgtctcccgc atggtcatgg cctcatacgt ggactacctg
      961 ctgaccaagg tcaaggagac ccagtttgag gagcgcaggc gctactccag ggagctgcac
     1021 gaccgccttg cacaccagat gacggtcgta acccaaagtc tggacctcta ccggacattc
     1081 agggagagcg acgccgagcg cgcggagaga cggctcgccc tcgcccaaga gtcggccagg
     1141 gagtcgctgg atctgatgca cgagttcgct aaagagctca ggctcttgga gacgtcggac
     1201 ggactcaaga tcgcgctgga gaacctcatg aggataagcg tacccgaagg tgtgtactac
     1261 gaggtctccc tggaaggtga ggagcagcac ctccccgact acgtgcgcga ccagctctac
     1321 atggtcttga gggaaggggt gcgcaacgcg gccgcccact ccggttccga ccggatcgag
     1381 gtcgaagtcg ggatcacccc gaagcatgtc acggcccgcg tacgcgacca cgggactggt
     1441 ctcaacgtcg aggacggtac caccgaaggg ctgggtttga gctcaatgaa ggaacgcgtc
     1501 tcgttgctcg ggggctcatt cgggctggtc cccgagccag gtggaggcgc gaaggcggag
     1561 gtcagcctgc ccctgatgcg ggagaagata cgatagacgc ccgccccgta accggccgcc
     1621 ctataagggt gctccttgcc gacgaccacg ccctggtgcg ccagggcatg gccgagatgc
     1681 tctccaccga cgaagggata gaggtggtcg gggaggccgc ggacggaagg gaggccgtgg
     1741 acctcgcccg caagcacggg ccggacgcgg tcgtcctcga cgtggagatg ccggtgatga
     1801 gcgggcaggt ggccttacgg aagctactcg acctccggcc gccgccgaag gttgtcatcg
     1861 tcaccgtctt cgccgacgaa tcccacgtgc gcgagctcct cgcgctcggg gcgagcgcct
     1921 acctctccaa gaacgcctcc atgcgggatt tgatctccac ggtgcgctcc gtcgtcgagg
     1981 accgggaagg gcagaacgag gacgacgtga tcctgttcgt gccccgggcc gcgttcgaca
     2041 aggacgctcc caaggcgagc gagctctccg gccgggaagc ggagatcctg atgctggcgg
     2101 cccgcgcccg ctccaatcgc gagatagccg aaaggctcca tctctcagag accacggtca
     2161 agcgacacct ctccaacgtt tacgaaaagc tgggcgtcca cacgagggcc gaggcggcga
     2221 ggaaagcgct atccgagggt tggatctccg cctgggacgt ctccagagaa gactagcgtc
     2281 ctcgacgagg ccgcaaggtt cgtatccgat
//
LOCUS       DATMJA010000190         4523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_558245, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000190 DATMJA010000000
VERSION     DATMJA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558245"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VNT12_04495"
     CDS             complement(<1..572)
                     /locus_tag="VNT12_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HWI45412.1"
                     /translation="MNQLRWDPTLREWVAYATHRQDRTFLPPAEYCPLCPTKPGGFPT
                     EVPREHYDIVVFENKFPSFSPNASAPDEEGSGLMPTAPGRGICEVVLYSDDHDATLAT
                     LSERRIGNLIEVWADRYEELGTSEEVEYVFIFENKGEAIGVTLHHPHGQIYGYPFIPP
                     RPAKELEAAREYRAQDEGRCLHCDLLSQEH"
     gene            complement(569..967)
                     /locus_tag="VNT12_04500"
     CDS             complement(569..967)
                     /locus_tag="VNT12_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45413.1"
                     /translation="MISSEVYNLRERRSYCQRPSFSPPLRFGNIRRSRTSSASEGLPL
                     VGYTWWPLFSLLDWAYREGEKPAEDYLWHMGMYDLEPDGKGGFLRVETPVVRAFWTAT
                     KRTSEPLSKEREQKEDGRYIIFYTFGEEED"
     gene            complement(984..2585)
                     /locus_tag="VNT12_04505"
     CDS             complement(984..2585)
                     /locus_tag="VNT12_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI45414.1"
                     /translation="METETDVLVVGGGLGGVAGALAAVRLGRRVLLTEETDWIGGQLT
                     AQAVPPDEHPWIEEMGCTASYRRLRNGIRDFYRRNYPLTTAARALEHLNPGGGLVSRL
                     CHEPRVALSVLEGMLAPYRSGRRLRVLLRHRPVSAESEQDLITAVTLLDEETGEPLTV
                     HASYVLDATELGELLELAGVEHVVGAESQDETGEPHAVPGDPNPLDQQAVSWCFPLDY
                     LPNEDHTIEKPQEYDFWRGYRASFWPAPQLSWTDVYPDTLQPRHRAIFHEEPFFAGRD
                     GSDLWHYRRILSRENFTGDLLDSDITLVNWPQIDYWLGPLVGGGEAERHEHLRRARQL
                     SLSMLYWMQTEAPRLDGGVGYRGLRLRPDVVGTTDGLARCVYVRESRRIRAEFTVLEQ
                     HVGVEARGGSKGAQYFHDSVGIGAYRIDLHPSAAGRSYVDVSSWPFQIPLGALIPIRV
                     RNLLPACKNLGTSHITNGCYRLHPVEWNVGEASGALAAYCLDKDLVPRQVHDRAQSLK
                     DFQRLLVDVLRFELAWPEPQRYDSL"
     gene            complement(3031..4374)
                     /locus_tag="VNT12_04510"
     CDS             complement(3031..4374)
                     /locus_tag="VNT12_04510"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HWI45415.1"
                     /translation="MLFQRKDQALKVAFIGAGSVVFIKNLLTDTLSFPELHGTTIALH
                     DIDPERLETAGMMARWTSDQLGAGAKVEEHLDRRRALEGADFVVNMVQIGMHEATLLD
                     FEIPKKYGLKQTIADSMGIGGIFRGLRTIPFLLDLAAEMRELCPKALLLNYTNPMGIL
                     MEALYIASPKTKSVGLCHSVPYTVREIASYIGVEHEELLYECAGINHIAWMTRLEIEG
                     KDAYPLLFAALEDPDVYAKDRVRFELMRRFGYFVTESSEHNAEYVPYFLKDDGLIDLY
                     DVPVDEYIRRSEENLVQYAETRRKLLAGEDFTLGRSVEYGSLIIHSLVSGEARAIYGN
                     VENTGLVTNLPEGCCVEVPILVDDTGLRPCYVGDLPPQCAATCAPHTAVQNLTVRAAL
                     EGRRDHVHHAAMLDRHASSVLSLDEIAAMVDELIEAHGEAMPEGVRISGALRRGA"
BASE COUNT          789 a         1430 c         1420 g          884 t
ORIGIN      
        1 tcgtgctcct gggagaggag gtcgcagtgc aggcagcggc cttcgtcttg cgccctgtac
       61 tctctggctg cttccagctc cttcgcggga cgcggcggga tgaatgggta gccgtagatc
      121 tgtccgtgcg ggtgatgcag ggtcacccct atggcctcgc ccttgttctc gaagataaag
      181 acatactcga cctcttcgct ggtgccaagc tcctcgtagc ggtcggccca gacctcgatc
      241 aggtttccga tgcggcgctc gctcagggta gccagcgtcg cgtcgtggtc gtccgagtag
      301 aggacgacct cacagattcc acgtcccggg gccgtcggca tcaggccgct gccctcttcg
      361 tcaggtgcgg aggcgttcgg ggagaacgac ggaaacttgt tctcgaagac gactatgtcg
      421 taatgctccc tgggcacctc tgtcgggaag ccgcctggct tcgtcggaca caggggacag
      481 tactctgcag gcggcagaaa cgtacggtcc tgacggtggg tggcgtaggc cacccactcg
      541 cgcagcgtcg ggtcccagcg gagctgattc aatcctcttc ctccccgaag gtgtagaaga
      601 tgatgtagcg gccgtcttcc ttctgctcac gctctttact caacggctcc gatgtcctct
      661 tcgtggcggt ccaaaacgcc cttactacgg gcgtctcgac ccgaaggaaa ccccctttac
      721 cgtcgggctc gaggtcgtac atacccatat gccacaggta atcctccgcg ggcttctccc
      781 cctcgcggta agcccagtct agcaggctaa agagaggcca ccaggtgtaa cctacgagcg
      841 gcagcccttc agacgccgag gatgtccggc tccgccttat gttcccgaaa cggagaggag
      901 gcgagaatga cggccgctga cagtaagatc tgcgctcgcg caggttgtac acctcggaag
      961 agatcaagcg gattccctat tgctcaaagg gagtcgtagc gttgcggttc tggccaggcc
     1021 aactcgaacc gcaggacgtc aacgagcaag cgctgaaagt cctttaggct ttgcgctcga
     1081 tcgtgtacct gccgcgggac aaggtcttta tccaggcagt aggcggcgag cgctcctgag
     1141 gcctcaccga cgttccactc gacgggatgc agcctgtagc aaccgttggt gatgtgggaa
     1201 gttccgaggt tcttgcaagc cggcagcagg ttcctgacgc gaatcgggat cagggcgccg
     1261 agcgggatct ggaaaggcca gctgctcaca tccacgtagc tacgtcctgc ggcgctggga
     1321 tgcagatcta tgcggtaggc gccgatgccc acgctgtcgt ggaagtactg cgctcccttc
     1381 gacccgcctc gcgcctcgac gcccacgtgc tgctccagca ccgtgaactc ggccctgatg
     1441 cggcggctct cgcgcacgta tacacacctg gcgaggccgt ctgtggtgcc gacgacatcg
     1501 ggacgaagac gcaggccccg gtatcctacg ccaccgtcga gtctgggcgc ttcggtctgc
     1561 atccagtaca gcatcgagag gctgagctgc cgcgcgcgtc gcagatgttc gtgtctctcg
     1621 gcttctccgc cgcctacgag cgggccgagc cagtagtcta tctgcggcca gttgaccagg
     1681 gtgatgtcgc tatcgagcag atcaccggtg aagttctcgc gggatagtat gcgccggtag
     1741 tgccagaggt cggagccgtc acgacccgcg aagaaaggtt cttcgtgaaa gatcgcgcgg
     1801 tggcggggct gcagggtatc ggggtagacg tcggtccagg agagttgcgg ggcaggccag
     1861 aagctcgccc ggtagccacg ccagaagtcg tattcctggg gcttctctat tgtgtggtcc
     1921 tcgttgggca agtagtcgag aggaaagcac cagctcaccg cctgctgatc cagggggttc
     1981 gggtctcccg gcaccgcgtg gggctccccg gtctcgtcct ggctctcagc cccgaccacg
     2041 tgctctacgc cggccagctc cagcagctct ccgagctccg tcgcgtcgag cacgtagctg
     2101 gcgtggacgg tcagcggctc gccggtctct tcatcgagaa gggtaacggc cgtgattaga
     2161 tcctgttcgc tctctgctga gacaggccga tggcggagca gcactcgcaa cctgcgtccg
     2221 gagcggtagg gggcgagcat cccctcaagg acggagaggg ctacacgcgg ctcgtggcag
     2281 aggcgactca ccagcccgcc tccgggattg agatgttcca gggcgcgggc tgcggtcgtg
     2341 aggggatagt tgcgcctgta gaaatccctg atgccattcc tcaaccggcg gtagctcgcc
     2401 gtgcagccca tctcctcgat ccagggatgc tcatccggcg ggaccgcctg ggcggtgagc
     2461 tggccgccga tccaatccgt ctcctcggtg agcaggaccc tccggcccag gcgcacagcg
     2521 gccagggcac cggccacacc ccccagtccc ccacccacga ccagaacgtc cgtctcggtc
     2581 tccaaaggcg gctacacccc tcgtcgtcgg ggcagccagg ctaccacttg tgatggacct
     2641 cttcgcgcac gtggctctcg tagatctccc gcgctcgttg cataacctcc tgggagagcg
     2701 gaggcatctc ggctgcggcg acgttgtcct cgacttgtgc agggttcttg ccgcccggga
     2761 tggcgcagag gcgccacggc gccttcgacc tggtaggccg cggttgccgt gccccacaag
     2821 aagttctcag ggaaacgcat agctctttcc tctccgttcc gtactacacg gcgaccggca
     2881 tctacaccgg gtggaacttc ggcggccggg tagtcgccga ggcggaagtg atcgcgcccg
     2941 tccactatcc ccgagaagaa cgacatcgga accgtcccta cgccttcagt ccttcaggct
     3001 gccgaaggtc tcgccagtaa cgttgcagga tcaagcaccg cgacggagcg caccagagat
     3061 cctgacacct tcgggcatgg cttcgccgtg ggcctctatt agctcgtcca ccatcgctgc
     3121 tatttcgtcg agagagagca cactcgaagc atggcggtcg agcatggcgg cgtgatggac
     3181 gtgatcccgc ctaccctcca gcgccgccct gaccgtgagg ttctggaccg ccgtgtgcgg
     3241 ggcgcaggtt gcggcacact gcggcgggag gtcccctacg tagcagggac gcagaccggt
     3301 gtcgtcaacc aggatcggca cctcgacaca gcaaccttcg gggaggttcg taactaggcc
     3361 ggtgttctcg acgttgccgt atatggcccg cgcctcgccg ctaacgagtg agtggatgat
     3421 gagcgaaccg tactcgacgc tccggcccag cgtgaagtcc tctccggcga gcagtttgcg
     3481 acgggtctcg gcgtactgga cgaggttctc ttcgctgcgc ctgatgtact cgtctacagg
     3541 cacgtcgtag aggtcgataa gcccgtcgtc cttgaggaag tagggtacgt actcggcgtt
     3601 gtgctcgctc gactcggtga cgaagtaccc gaagcggcgc ataagctcga atcgcaccct
     3661 gtccttggcg tacacgtcag ggtcttccag cgccgcgaac agcagagggt aggcgtcctt
     3721 gccttcgatc tccaggcgcg tcatccaggc gatgtggttg atgccggcgc actcgtagag
     3781 aagctcctca tgctccaccc cgatgtatga ggctatctcc cggacggtgt aagggacgct
     3841 gtggcagagc ccgacgctct ttgtcttggg gcttgcgatg tacagcgcct ccatcaggat
     3901 gcccatcggg ttcgtgtagt tcaggagcag cgctttcgga cagagctctc gcatctcggc
     3961 ggcaaggtcg agcaaaaaag ggatggtacg cagtccgcgg aagatgccgc cgatgcccat
     4021 cgagtcggcg atcgtctgtt tgagtccata cttcttgggg atctcgaagt cgagcagggt
     4081 ggcctcgtgc ataccgatct ggaccatgtt tacgacgaag tcggcccctt cgagcgcccg
     4141 cctccggtcg agatgctcct cgactttggc accggcgccg agttgatccg aggtccagcg
     4201 ggccatcatg cccgcggtct ccagacgctc tgggtctata tcgtgcagag cgatggtggt
     4261 tccgtgcagc tcggggaagc tcagggtgtc tgtcagtagg ttcttgataa agacgacgct
     4321 ccccgcaccg ataaacgcta ccttcaaggc ttgatccttt ctctgaaaga gcaacttgcc
     4381 atcacctatg cacaggttac ttcaacacgg ccaccccggc aggagcgatg tcgatgtggt
     4441 gcaacccggt ctcagcccga tgctgtccgt atgcgtaacg cgtcccctgc aatcccgcac
     4501 ctctatctgg cgtgtgcccg cat
//
LOCUS       DATMJA010000191         7034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_617674, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000191 DATMJA010000000
VERSION     DATMJA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617674"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VNT12_04515"
     CDS             <1..189
                     /locus_tag="VNT12_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45416.1"
                     /translation="PRLEQLPHLKARLKALEDIHENKEVRWITDDCQQVPQGLWQSST
                     SKSSNSPALLYDCGTGAR"
     gene            complement(530..1465)
                     /locus_tag="VNT12_04520"
     CDS             complement(530..1465)
                     /locus_tag="VNT12_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45417.1"
                     /translation="MSGSPVGRFARRLVRLYPRTWRDRYEEEFVALLEESAGSIGDVF
                     DVALGAMDAWMKPQVVSETSGARLLMVARLRGSVLVVLWAWALLAVAGVGFQKMTEYD
                     DFVRVARENAVVGVAFDAVVGGAVLALAAVLIGGAPIGFAATRRALAEGRRDVPLLLS
                     VPPLSLAAFVGYVLMLTKIIYPALGALAVHDAVNVALFLSLAGAFLVAAVVSVAAVSA
                     AVGRSGVGERPFRFALASAAVAAVGMVAVLVGTVVWGLALRAQAPALFTGDDGILATP
                     TYATWGVIVSAMFLSCCVAVVAVLRGLSTGRSFVP"
     gene            complement(1462..1779)
                     /locus_tag="VNT12_04525"
     CDS             complement(1462..1779)
                     /locus_tag="VNT12_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWI45418.1"
                     /translation="MGHEVSELGRFSDPALFVLVSLAGGEKHGYAMMEDIQKTCGTRL
                     GPGTLYGALSRLEKRGLVEPLYYGERRRPYRLTAAGASALEDRLRELEGVARTGLGRL
                     ATA"
     gene            complement(2046..2619)
                     /locus_tag="VNT12_04530"
                     /pseudo
     CDS             complement(2046..2619)
                     /locus_tag="VNT12_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897899.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2775..3734
                     /locus_tag="VNT12_04535"
     CDS             2775..3734
                     /locus_tag="VNT12_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI45419.1"
                     /translation="MSQHENSARTATDPAARLPATLRLGPVHLTVSDLDRSVAFYEDA
                     IGLRVHRRKDGVAAMGVGEEDLIVLYEEPRARRAGRHAGLYHYALLFPSREELAHAAL
                     RLAETKTPIQGASDHGTHEAIYLPDPDGNGIELAADRPREAWPDLANTGWDGGPTPLD
                     MDGLLAVVEGEEPRREAWPGLAIGHLHLHVGDVHRGLAFYHDVLGFDVMMLFPGQAAF
                     VSAGGYHHHLGFNVWRGEGVPPAPPGRVGLRHWTVWLEDPGEVAAVRERVRTAGIGAE
                     EAEGGGFVVRDPWGIAARFVARDFPEGVRKREVGTDPGGFGTT"
     gene            3761..4132
                     /locus_tag="VNT12_04540"
     CDS             3761..4132
                     /locus_tag="VNT12_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI45420.1"
                     /translation="MNVEGITWHAIVLDPEPFAATKALLTETFGLAPAVDTEGFAMFH
                     LPNGTTLELYAPHAVPDYGFNDGGVAFGFRVDDIEAASAAVEAAGCELLGEITRMEQL
                     AYSYRDFRGPDGRVYGLNEQK"
     gene            5208..6716
                     /locus_tag="VNT12_04545"
     CDS             5208..6716
                     /locus_tag="VNT12_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWI45421.1"
                     /translation="MSVCRARSLSLFGIDALPVEVEVDVSSGLPGFSIVGLPDAAVQE
                     ARERVKVAISNSGYKFPTKKVIVNLAPANLRKEGPAFDLPIALGILAASGTLPAGGLE
                     DAGAVGELSLDGGLRGVRGALSMAEGARREGLARLLVPERDAPEAAAIGGVEVYGMRS
                     LREAVEFLGDSARVSPAEPVAEDGRTFEAGEDFADVAGQRYAKRALEVAAAGGHNVIM
                     SGPPGSGKTMLARRMPGILPPLTMEERIEVTKVHSASGEGNGTLVTRRPFRAPHHTVS
                     TSGLAGGGSNPRPGEVSLAHHGVLFLDELPEFSRTALEALRQPLEDGHVTISRVAATL
                     SYPARVTLLCSMNPCPCGYAGDRRRVCRCTVGQIERYQGRISGPLLDRMDLFVDVPRL
                     TRQELREERQAEESRFIRERVSGGREVQERRQGFPNSALSGRRLREVCALKGAAETLF
                     SRAVDRMGLSGRGHDRVLRVARTVADLSGEEEIAAEHVAEALNYRRSHLVGR"
     gene            6706..>7034
                     /locus_tag="VNT12_04550"
     CDS             6706..>7034
                     /locus_tag="VNT12_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45422.1"
                     /translation="MEDDDRAKYLFLSLLQARVGSSLISHLGNLDPEAVLRTPVGELA
                     GAAKISERASRAFQELQMAFDPGRMQERLAAKGIEALTPVDEAYPAPLRSIPDPPPAV
                     FVNGVVP"
BASE COUNT         1339 a         2202 c         2283 g         1210 t
ORIGIN      
        1 ccgcgcctcg aacagctgcc gcacctcaaa gcacgcctca aggcgctgga ggacatccac
       61 gaaaacaagg aggtgaggtg gataacggac gactgccagc aggttccgca gggactgtgg
      121 caatcatcaa cctccaaatc atcaaattcc cccgcgctgc tctacgactg tggtactgga
      181 gcccggtagc cttcgtcaag gagtgagaag cggtgaaagg taacgctggt gccgagagcc
      241 tgtatgggca ggatggtaac tacgcgccca actccctaga ggggttagcg gcaacgactc
      301 cttaaacggg agtgccggca ccgacaccaa gataaccaac gcaacctgga agtcgattgt
      361 agggtctccg cagggcgtta ggagaccgca aatggagcta ggaccagggc gtagcagctt
      421 cggccattat tcaccgaagt tcgtagaagg taaattctgc gatgttcgcc ttgacggagt
      481 tctccgccac tcgggccagc aggcccacga ggaaggacat gcctgcatcc taagggacga
      541 aggagcgccc ggtgctcaac ccgcggagga ccgcgacgac agcaacgcag caagagagga
      601 acatagctga cacgatcacc ccccacgtgg cgtaggtagg ggtcgccagg ataccatcgt
      661 cgcccgtgaa cagcgcggga gcttgcgccc gcagcgctaa gccccagacg acggtgccca
      721 ccagcaccgc gaccatcccc acagcggcga ctgcggcaga agccagggcg aatcggaagg
      781 gccgctcccc aaccccggag cgcccgaccg cggcggagac cgccgccaca ctcaccactg
      841 ccgcgaccaa gaaagccccc gcgagggaca ggaagagcgc cacgttaacg gcatcgtgga
      901 ccgccagagc tcccaaggcc gggtatatga tcttcgtgag catgagtaca tagccgacga
      961 aggcggccaa tgagagcggg ggaacgctca agagcaacgg cacgtccctc cggccctcgg
     1021 ccagggccct gcgtgtcgct gcgaagccga tgggtgcccc gccgatgagg actgccgcca
     1081 gcgccaagac ggccccgccg accaccgcgt cgaaggccac gccgaccacc gcgttctcgc
     1141 gcgccacccg aacgaagtca tcgtactcag tcatcttctg gaaccccacg cccgccaccg
     1201 ccagaagagc ccatgcccac aacaccacta gcaccgatcc gcgtaacctc gcgaccatca
     1261 acaaccttgc tccgctcgtt tccgagacca cctgcggttt catccaggcg tccatagcgc
     1321 ccagcgcgac atcaaacacg tccccgatcg accccgcgct ctcttcaaga agtgccacaa
     1381 actcctcctc gtagcgatcc cgccacgttc gtgggtagag gcgaaccagc cgcctggcga
     1441 accgtcctac ggggcttcca ctcacgcggt cgccaacctt cctagcccgg tacgcgcgac
     1501 tccttcaagt tctcgcaacc gatcctccag ggccgaagcg cctgccgccg ttaggcggta
     1561 tggcctgcgc cgctcaccgt aatacagggg ctcgaccaaa cctctcttct ctaagcgcga
     1621 gagagcgccg tagagagtgc ccggacccaa cctggtaccg cacgtcttct gtatgtcctc
     1681 catcatggcg tagccatgct tctcgccgcc ggccaggctc accaagacga agagcgccgg
     1741 gtccgagaac cggcccaact ctgacacttc gtggcccatc gacacctccg aggtaaactt
     1801 acgtaccacg tattagtacg tgacgcgtaa taatacagcg atagggcctg tacgtcaaca
     1861 tgccacggca aggatcagcg tgccacggtt acaacgcaaa cctagagctt cacccttcat
     1921 acacccgagt gcgtggaaag agtgtcctcc gaaggtcgcg taattctcat aaaactctca
     1981 acctgttgct aggttagtac cgaatactcc gcgcaagtgt ctgactaaga ggccgcgcca
     2041 ataggtcatg ggcggcgatg gggacgtccc tcccaacggt agcgaaccca cgcttttcga
     2101 acgagccttc ctacgataat gatcacgttg gagaaggcca cccaaaagcg gtgagtcgtg
     2161 acggttggcc ggggcggtag cgaacccaag cgggatgcca tgtgcgtcga cggctaccga
     2221 gcgttttatg ccctgctttc ttctgtccac cgggcttctg actgccttct ctcctccgca
     2281 gggagccttc gtgaggcagc aatcgacggc cacatcggct acatcgaggc cgatggtccg
     2341 gtcgtaggct tggcgcgcca tctcctctag attgtccatc actccggcct agatccactc
     2401 gtcgcgtctg cggcgcaggg tggttgccga acacgactcg tcagcgattc gccaataggc
     2461 gcaaccgaac accagtatct gaagcagcct ctcttcgaga agctgatcgg gtatgcgtcg
     2521 gcggtggcag cccaaaggat ggtccacctt tctttcgggc agcaaggcac gagactgctc
     2581 ccagataggc tccatgatgt atggtgagat ggcgggcatg agcatactcc ttcgcatcgg
     2641 gaagcttcga gaactccccg aatgatcgag catcttgtgc tcgccttaca agtgagtgtg
     2701 ctgcgtccct attggcgcaa gctctaagct agcgtgtcct cgaacgtcta gggcgaacac
     2761 gaaggaggat cgacatgagc cagcacgaaa actcggctcg caccgctacc gacccggcag
     2821 cgaggctgcc cgcaacgctc cgactgggcc ccgtgcacct gaccgtctcc gaccttgacc
     2881 gctcggtagc cttctacgag gatgccatcg gcctgagggt acaccgcaga aaggatgggg
     2941 tggcggcgat gggggtgggc gaggaggacc tgatcgtcct ctacgaggag ccaagggcgc
     3001 ggcgggcggg tcggcacgcg ggcctctacc actacgcgct gcttttcccc tcccgcgagg
     3061 agcttgcgca tgcggcgctg cgcctggccg agaccaagac cccgatccag ggcgcctccg
     3121 accacggcac ccacgaggcc atctatctgc ccgatcccga cgggaacggg atcgaactcg
     3181 ccgccgaccg cccgcgcgag gcttggccgg acctcgccaa caccggctgg gacggtgggc
     3241 ccacgccgct ggacatggac ggcctgctgg cggtcgtcga gggcgaagag ccgcgccgcg
     3301 aggcctggcc ggggctcgcg ataggccacc tgcacctgca cgtcggcgac gtgcaccgcg
     3361 ggctggcctt ctaccacgac gtgctcggct tcgatgtcat gatgctcttc cccggccagg
     3421 ccgcgttcgt ctcggccggc ggctaccatc accatctcgg cttcaacgtc tggcgcggcg
     3481 agggcgtgcc gcccgctccg ccgggcaggg tcggcctgcg ccactggacg gtctggctcg
     3541 aggacccggg ggaggtagcc gcggtgcgcg agcgggtgcg tacggcgggc atcggggccg
     3601 aggaggcaga aggcggcggc ttcgtggtgc gcgatccttg ggggatcgcg gcacgcttcg
     3661 tcgctcgcga cttccccgaa ggcgtaagga agcgggaagt cggcacagat ccaggaggtt
     3721 tcggcactac gtaagagacg acgattggga ggaaaagacc gtgaacgtag aaggcataac
     3781 ctggcacgcc atcgtcctcg atcccgagcc gttcgccgcc accaaggcgc tgctgaccga
     3841 gactttcggc ctggccccgg ccgtggacac cgagggcttc gccatgttcc acctgccgaa
     3901 cgggacgacg ctggagctgt acgcgcccca tgccgtcccc gactacggct tcaacgacgg
     3961 cggagtcgct ttcggcttta gggtggacga catcgaggcc gcctctgccg cggtggaggc
     4021 ggcggggtgc gagctgctag gcgagatcac ccgcatggag cagctcgcgt actcctaccg
     4081 agacttccgc ggtccggacg gccgcgtcta cggcctcaac gagcagaagt agcgcgcaca
     4141 cgaaacggcg ggcacgcgtc acgagtgacg caaagacggg gcctaccggc ggtttgcgga
     4201 cgggcttcgg cccgtcgacc ccagcccttt cgcacttcta cctgcatggg cgcggcacca
     4261 gggccggcgc ccgctggggg cggcgcgtca gcggcggatg atcggcagca ccagccggct
     4321 cggtgctcag gacgccgtcg gcggtgtttg tccggccgga ggcacttggg cggatgggcg
     4381 ccgaaggact tgaccgctcc cctaacgtgg ttgaccagct gcgtgaggca accgatcaat
     4441 gcttgcctgg agcggattaa ggccatgtgg gcccggccct cttcgcttcg gtgcttgagt
     4501 ggatacaaca gcatcgggtc aactcgtgcc aggcgagcca ggttctcggc atttacctca
     4561 tctgttttac gtttgttggc gcagatgagc ctcagcttcc tggcattggc taccaagacc
     4621 tcgtggccac actcctctag caccctgctc gcccagggtg agtgggttcc agcctcaatg
     4681 gctataggca taggctgctc tgaggcgaag cgtcgccaga aggcctcggg gctactattc
     4741 atggtgggcc gatcctcctt ccattttggg cgttttagag cccgtcgaac gtttgcagac
     4801 cctctaaggg tactgcgtat ggggggtcga ccttctcatc ttccaagaac tccgttaggg
     4861 cgaagttcgc aaaatcttcc ttccacgcac tcgggtgaat aggtcagttc acatcagcgt
     4921 cgtcgcgcag aagccacctt ccaacgaggc ggtgaagtct gtgcagtaga ggaacaggga
     4981 gtcttcctct tcttctctcg cttgacttcg ccaggcgact tgtcaaccag ttagccaagg
     5041 atcaggcaca agcgaaggct gatgtcatgg ctcagtgtat aaaataataa acaatcgtat
     5101 atcatcatag acgcaaatgc tgtatactgt aggggctctg tgggggcatg ttgtgtcgag
     5161 gtggtggagt aaggggtcac ggcgatggct tgtgggtgtt gtggttgatg tccgtctgcc
     5221 gggcgaggag cctgagtcta ttcgggatag acgcgttgcc tgtggaggta gaggttgatg
     5281 tgagctctgg cctaccgggt ttctcgatcg tgggtctgcc tgatgcggcg gtgcaggagg
     5341 cgcgggagag ggtgaaggtg gccatctcca acagcggcta caagttccct acgaagaagg
     5401 taatagtgaa cctggctccg gcgaacctca ggaaggaggg gcctgcgttc gaccttccga
     5461 tagcgcttgg gatcctcgcc gcgtccggca cgctgccggc ggggggattg gaagatgcag
     5521 gcgcggtggg ggaattgtcg ctagatggcg gcttgcgggg tgtaagggga gcactatcga
     5581 tggccgaggg cgccaggcgg gaggggctag cgcgtctgct cgtccctgag cgagatgcgc
     5641 ccgaggcagc ggccatcggc ggagttgagg tctatggaat gcggagtctg agggaggctg
     5701 tcgagtttct aggggactct gcgcgggtct ctcccgctga accggtggcc gaggatgggc
     5761 ggactttcga ggcgggcgaa gactttgccg acgtagcagg tcagcgctac gcaaagcggg
     5821 ctctggaggt ggctgcggca ggggggcaca acgtcatcat gagtgggccg cctggttccg
     5881 ggaagaccat gctggcgcgc agaatgcctg gcattctccc cccgctcacc atggaggagc
     5941 gcatcgaggt gaccaaggtc cacagcgcga gtggggaggg caatgggacg ctcgtcacac
     6001 ggaggccgtt cagggcgccg caccacaccg tgtcgacctc ggggctcgcg ggagggggga
     6061 gcaacccgcg gcctggggag gtgtcgctgg ctcaccacgg cgtgctcttc ctcgacgaat
     6121 tgcccgagtt ttcccgtacg gcgttggaag ctctacgtca gcccctggag gatgggcacg
     6181 tgacgatctc gcgggtggcc gcgacgctca gctatcctgc gcgggtcacg ctgctctgtt
     6241 ccatgaaccc atgtccctgc ggctacgcag gggacaggag gagggtctgc cggtgcacgg
     6301 ttggccagat cgaacgctac cagggccgaa tctccgggcc cttgctcgat cggatggacc
     6361 tcttcgtcga cgtgccgcgc ctcacgcgtc aggagttgcg cgaggagcga caggcagaag
     6421 aatccaggtt cataagggag cgggtcagcg gggggcgtga ggtccaggaa cgccgccagg
     6481 gcttccccaa ttccgcgctc tccggacgca ggctgcgcga ggtatgcgcc ctcaagggtg
     6541 ctgcggagac actcttttcg cgggcggtgg accggatggg actctcggga agggggcatg
     6601 acagggtgct cagggtggct aggaccgtcg ccgacctctc gggcgaagag gagatagccg
     6661 ccgagcacgt cgccgaggcg ctcaactacc ggaggtcgca tctagttgga agatgacgac
     6721 cgggcaaagt acttgttcct ctcgctcttg caggccaggg tagggagcag cctcatctcg
     6781 cacctcggta acctcgaccc cgaggccgtg ctccggactc ccgtcggaga gttagcggga
     6841 gcggcaaaga tctccgagcg ggcttcgcgc gctttccagg agttgcaaat ggccttcgac
     6901 cctggccgga tgcaggagcg tctggcggcg aaagggatcg aggccctcac gcctgtcgat
     6961 gaggcttacc cggcaccgct gaggagcata cccgaccctc cgcccgcggt ctttgtgaac
     7021 ggcgttgtcc caga
//
LOCUS       DATMJA010000192         2493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_84433, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000192 DATMJA010000000
VERSION     DATMJA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84433"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VNT12_04555"
     CDS             <1..1572
                     /locus_tag="VNT12_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HWI45423.1"
                     /translation="GGKTPAEARQIVRDQVMGPLEEIDFDGPAHFTRKSREMGVSFNI
                     DQTVDKAYAVGREGNILHRLSKRLRASFGGATIRPDIDYQPAKARGEMREIASQVDRP
                     PTEANVKIYGSEVEVAKSRDGYELNLAATLASLDSAIKDMSGKARLRGDVLDPGVVTA
                     EAEAAAKKARGALSEQLMLKAEGKSWTLSPADLGSVLDVTKQDGKIDVSLNRDHLDGR
                     LTNVYNDLTIKPVEASYDFDADGDIFVKPSHEGRSVEGEKLLDSIQSGLFEGKREYQV
                     PITVAKPHYTTAELEAKKPSELQGTYRTNYTATTDQGQTRVENLKIASGAISGTFVAP
                     GGTFSMLDHVANLDYFQTHVIVNGAETVDEGGGLCQVTSTLYNAALYAGMEITERTPH
                     YSQLPYIRPGMDATVWYGGPGPSDDLDMQFKNTSDGYVLLQEYVSNDGYIYANVYGVP
                     DKVKVEMSSEPIFKAEDASKWVTYYERTRNGKVVYRDQWETEYGALIDDEGKKIPTPI
                     VPVAEIDGTYLGPQF"
     gene            complement(1789..>2493)
                     /locus_tag="VNT12_04560"
     CDS             complement(1789..>2493)
                     /locus_tag="VNT12_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45424.1"
                     /translation="EVGPLLEHCRNILEISLEAADQAGRPLEMTDFGGGFGVPYFEKM
                     SEFDLEGFADGFRDLLSSYRQDPRLEGCRFLFELGRYLVADAGIYITRVVDVKETRGK
                     TFVVTDGGMNHHLTATGNMGQVFRKAYPLLNLTRMSGVPEESVAVAGPCCTPLDVFGN
                     NIPLATPEAGDLIGVFYSGAYGYSASNLGFLSHPTPAEVLLWRGGAHLLRPGGRPEDV
                     LSGQRALSKPESSVPG"
BASE COUNT          532 a          767 c          780 g          414 t
ORIGIN      
        1 ggcggtaaga cacctgccga ggcgcgccag atcgtcaggg accaagttat gggtccgctc
       61 gaggagatcg acttcgacgg ccccgcgcat ttcacccgca aatccaggga gatgggtgtt
      121 agcttcaaca tagaccagac cgtggataaa gcctacgccg tcgggcgcga aggaaacatc
      181 ctccaccgtc tctccaagcg tctgcgcgcc tcattcggcg gggcgacgat acgtcctgac
      241 atcgactacc agcctgcgaa agcgcggggc gagatgcgcg agatcgcctc ccaggtagac
      301 cggcctccaa cggaggccaa cgtaaagatc tacggctcgg aggtggaggt cgcgaagtcc
      361 cgcgacggct acgagttgaa cctcgcggcc accctggcga gcttggacag cgccatcaag
      421 gatatgagcg ggaaggcccg cctcagaggc gacgtgctcg accctggggt agtgacggct
      481 gaggcagagg cggcggcgaa gaaggccagg ggagctctct ccgagcagct gatgctcaaa
      541 gcagagggga agagttggac cctctcaccg gcggatctgg ggtcggttct cgacgtcacc
      601 aagcaggacg gtaagatcga tgtcagcctc aaccgcgacc atctggacgg ccggctcacc
      661 aacgtctaca acgaccttac gatcaagccc gtcgaggcca gctacgactt tgacgcggac
      721 ggcgatattt tcgtcaagcc cagccacgaa gggcggagcg tcgagggcga gaagctactc
      781 gactccatac agagtggcct cttcgaaggc aagcgcgagt accaggtgcc tattacggtg
      841 gccaagccgc attacactac cgccgagctc gaggccaaga agccttccga gctacagggc
      901 acttacagaa ccaactacac ggcgaccaca gaccagggcc agacgcgggt ggagaacctc
      961 aagatcgctt ctggtgccat tagcggtacg ttcgtggcgc cgggcgggac attctcgatg
     1021 ctcgaccacg tcgccaacct cgactacttc cagacgcacg tcatcgtgaa cggcgcggag
     1081 actgtggacg agggaggcgg tctctgccag gtcacctcga cgctctacaa tgcggccctc
     1141 tacgctggta tggagataac ggagcgcacc ccccactact cgcagttgcc ttacatcagg
     1201 ccgggcatgg acgcaacggt ctggtacggt ggcccggggc cctcggacga cctggacatg
     1261 cagttcaaga acacctctga cggctacgtg ctcttgcagg agtatgtctc gaacgacggc
     1321 tacatctacg ccaacgtata cggtgtacct gacaaagtca aggtggagat gtcgtccgag
     1381 cccatcttca aagccgaaga tgcctccaag tgggtaacgt actacgagag aacgaggaac
     1441 ggcaaggtcg tctatcgcga tcaatgggag accgagtacg gcgccctgat agacgacgag
     1501 ggcaaaaaga tcccaacccc catcgtgcct gtggccgaaa tcgacggtac ctacctggga
     1561 ccccagtttt gaagcagcgg tcgaggagat tcaacctcct cgaccgcttt cagcacgtca
     1621 gcacgctcag gcttacacct ccgcttgtca gcccggcggc tgcgccgcct tgtcagctag
     1681 gcctggaatg gcggagggaa gcttctacgg agaggctttt tgtcgtggcg aacgattcgt
     1741 gatcgagcac gagcggcgga ccgacactaa gagagctgaa atgctgacct agcctggcac
     1801 gctggactcc ggcttcgaga gagctctctg tccgctcaag acgtcctcgg gcctgccacc
     1861 gggacgcagg aggtgagctc ctccccgcca cagtagcact tcggcgggtg tggggtggct
     1921 caggaagccc aggttggaag ccgagtaacc gtaagctccg ctgtagaaaa caccgatcag
     1981 gtccccagcc tcaggggtgg ccaggggtat gttgttgccg aacacatcga gcggcgtgca
     2041 gcaaggtccg gccacagcaa cgctctcctc aggcaccccg ctcatgcggg taaggttgag
     2101 cagcggatag gccttcctga acacctggcc catgttcccc gtcgccgtga ggtggtggtt
     2161 catgccgccg tccgtgacga cgaaggtctt gcccctcgtc tccttcacgt cgacgactct
     2221 ggtgatgtag atgccggcgt cggccaccag gtagcgcccc aactcgaaga ggaaacggca
     2281 cccttcgaga cgcggatcct ggcggtagga ggagagaagg tcgcggaagc cgtcggcgaa
     2341 gccctcaagg tcgaactcgc tcatcttttc gaagtagggg accccgaagc cgccgccgaa
     2401 gtcggtcatc tccagcggtc ggccggcctg gtctgccgcc tccagcgaga tctccagaat
     2461 gttgcggcag tgctcgagga ggggcccgac ctc
//
LOCUS       DATMJA010000193         3183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_854666, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000193 DATMJA010000000
VERSION     DATMJA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854666"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VNT12_04565"
     CDS             complement(<1..199)
                     /locus_tag="VNT12_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWI45425.1"
                     /translation="MFTGLVEAVGRVSSIGEGEILRLSISAGRVSEGARAGDSVSVNG
                     ACLTVGAVEGGTFTFFAMPETL"
     gene            complement(180..1298)
                     /gene="ribD"
                     /locus_tag="VNT12_04570"
     CDS             complement(180..1298)
                     /gene="ribD"
                     /locus_tag="VNT12_04570"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWI45426.1"
                     /translation="MLQDSMMDLARCLAERGRYSVAPNPLVGSVVARDGSVLSEGWHS
                     RAGEDHAEIVALGKAGGASRGATLYVTLEPCNHYGKTPPCVEAILRAGISRVVVGHLD
                     PDPRMQGSSVRILREAGVEVDVLDDPAFERQNEQFLHHMQTGRPFVHLKLATTLDGRI
                     AASGGDSRWVTGKDARLRAHELRAEAGAVLVGANTVRTDDPMLTARDLPVPSPRITRV
                     VLDPHLTISPGSKLAKSAGETPVVVFAPEPALDGREKELERLGVEVVATPLSEEGLDL
                     VHVLDELGGRGLRGLLVEGGGETATRFVRMGLADKLTLFYAPKLLGSEGVPMIGALRA
                     TKVAESLRFSVSEVGKVGDDVAVTLYPGGEEERVHRAC"
     regulatory      complement(1300..1470)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1637..2308)
                     /gene="rpe"
                     /locus_tag="VNT12_04575"
     CDS             complement(1637..2308)
                     /gene="rpe"
                     /locus_tag="VNT12_04575"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWI45427.1"
                     /translation="MTFFGRLGDRVVGAPSILAADFSELAEEVRRAERGGAELVHFDV
                     MDNHFVPNLTVGPAVAASLAAAVDLPIDAHLQVTNPDNLIPSFIEAGVVSISVQPEVV
                     THLHRTLDLIRTGGCEAGVALNPTTPPESIEWTLPYLSYVNVMSVNPGFGGQAFIPEM
                     VEKVRRLKEITDVPVEVDGGITVETAPLMVRAGAQVLVAGSAVFKGDPAMEMRRIIEA
                     GQGAE"
     gene            complement(2305..>3183)
                     /locus_tag="VNT12_04580"
     CDS             complement(2305..>3183)
                     /locus_tag="VNT12_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45428.1"
                     /translation="FLIGLPFLLWRLKRSLAAQLLFGMMYLTTVIVYVPQIATFLGDN
                     VVLPGQIWRLAWPLQLAAVLTLGWLVWSATDYVAAWLRSLGPARLLAGALPILLVVAL
                     TAAAVPQARQGMESIQAHREASRKSGFYPSDPIFPWFRDEIRSSVVVLAPDIQSARIP
                     AYSYEANVVSRRGGLVLRVLPELQKRAPGQIEVPQGSLDVREFFSGTTLQQGVEILRR
                     NNVDYVMVAKDSQLTGSLEHLSGFTPVKTPSERYDLYAVDLQEVASKSGPPMSSPGAL
                     SIEWPSLDTRPRRQIS"
BASE COUNT          614 a         1057 c          971 g          541 t
ORIGIN      
        1 taagcgtctc gggcatggcg aagaaggtga acgtcccgcc ttcgaccgcg ccgaccgtca
       61 ggcaagcccc gttcactgag acgctgtcgc cagcgcgggc gccctcagac accctgccgg
      121 cagagatgct caggcgcaaa atctcgccct ccccgatcga ggagacacgc cctacagcct
      181 caacaagccc ggtgaacacg ttcctcctct ccgccagggt agagcgtcac ggcaacgtca
      241 tcgccaactt tcccgacttc cgagaccgag aatcgcagcg actctgccac cttcgtagcc
      301 ctcagtgctc cgatcatggg tacgccctca gagccgagga gcttcggcgc gtagaacagg
      361 gtcagcttgt cggcgagccc catacggaca aatcgtgtag ccgtctctcc cccgccctcg
      421 acgagcaggc ctcttaaccc acgtccgccc aactcatcca gaacatggac gaggtcgagg
      481 ccctcctccg aaagcggggt cgcaactacc tcgacgccaa gcctctccag ctccttttca
      541 cgaccgtcga gcgcgggctc aggagcgaag acgacgacgg gagtctctcc ggcgctcttg
      601 gcgagcttgc tccccgggct gatagtcaga tgtgggtcaa gcaccacgcg ggttatacgg
      661 ggtgagggca cgggaagatc ccgggccgta agcatagggt cgtccgtccg cacggtgttc
      721 gctccgacca gtaccgcccc ggcctcggcc ctgagttcgt gggcccgcag cctcgcgtct
      781 ttgcccgtaa cccacctgct gtcaccaccg gaggcggcga tcctgccgtc gagggtggtg
      841 gcgagcttga ggtggacgaa cggccttccg gtctgcatgt ggtggaggaa ctgttcgttt
      901 tgccgctcga acgcagggtc gtctagcaca tcgacctcga cgccggcctc gcgcagtatc
      961 cgtacgctac taccctgcat cctgggatca gggtccaggt ggccgacgac tacgcgcgag
     1021 atgccagccc gcaagatagc ctcgacacag ggcggtgtct tgccgtaatg gttgcagggt
     1081 tcgagcgtta cgtagagcgt agccccacgc gaagcccctc cggccttacc aagggccact
     1141 atctcggcgt ggtcctctcc tgccctggaa tgccagccct cgcttagcac gctaccatcg
     1201 cgcgcgacga cggatcccac gagagggttc ggcgcaaccg agtaacgccc gcgctcggcg
     1261 aggcaacggg caagatccat catcgaatcc tgcagcacgt cttcctttct cccatccgga
     1321 ctctgaccgt cggcgccgga ctacggctcg cagcgaatac gaaccttcac cggctcaacc
     1381 tccaaggagg ctcgcgggct ctccagcagg gtcacgaccc tgcaaggctc accgccggtg
     1441 cgggaattcc acccgccccc gaaagttacg cagcaagcta taccacagaa ggaccacggc
     1501 agcaaggaaa ggaatcaggt tacgggtcta tgaaagcgta cgccagcctg gcctgccgca
     1561 gtacgacccg gtaccacaat ctgaaccgga ccctgtcacg gtctactcag ggttccgtcc
     1621 tgccaggacc tgatccctac tccgcgcctt ggccggcttc gataatcctg cgcatctcca
     1681 tcgccggatc gcctttgaag accgccgaac cggcgacaag gacctgggcg cccgccctga
     1741 ccatgagcgg cgcggtctcg accgtgatgc cgccgtccac ctcgacgggg acgtcggtga
     1801 tctccttgag gcgcctgacc ttctcgacca tctcggggat gaaggcctgg cctccgaagc
     1861 cggggttgac gctcattacg ttcacgtagc tcaggtacgg cagcgtccac tctatagact
     1921 cgggaggcgt cgtcgggttg agggcgacgc cggcctcaca accgccggtt cggatcaggt
     1981 caagcgtgcg gtgcaggtgc gtgaccacct cgggctggac cgagatgctc acgaccccgg
     2041 cctcgatgaa agatgggatc aggttgtcag ggttcgttac ctggaggtgg gcgtctatgg
     2101 gaaggtccac cgcggcggcg agcgaggccg cgacggcggg gcccacggta aggttcggta
     2161 cgaagtggtt gtccatcaca tcgaagtgta cgagctcggc tccacccctt tcggccctac
     2221 gcacttcctc ggccagttcc gagaaatcgg ccgcgagtat cgaaggggcg ccgaccaccc
     2281 tatcaccgag ccttccgaaa aacgtcaaga gatctgcctc ctaggccgag tatccagaga
     2341 gggccattct atagaaaggg ccccaggcga actcatcggc ggtcctgact tcgaggccac
     2401 ctcctggagg tccacagcgt aaagatcgta cctctcactt ggtgtcttta caggggtgaa
     2461 gcctgacagg tgttcgagcg atccggtcag ctgtgagtcc ttggcgacca tcacgtagtc
     2521 cacattgttg cggcgcagga tctcgacgcc ttgctgcagc gtagtcccgc tgaagaactc
     2581 ccgcacatcc agggagccct ggggcacttc gatctggccc ggcgcccgct tctgtagctc
     2641 agggaggact ctgagcacca atcctcccct gcggctgacg acgttggcct cgtaggagta
     2701 agcggggata cgggcactct ggatgtcggg cgcaagcacg acaacggacg agcgaatctc
     2761 gtcgcggaac cagggaaaga tgggatcaga agggtagaag cctgacttgc ggctcgcctc
     2821 cctgtgcgcc tgaatagact ccataccttg gcgcgcctgg ggcacggccg cggcagtcag
     2881 ggcgacgacc agtaggatcg gtagtgcccc cgccagtaaa cgcgccggtc cgaggctacg
     2941 cagccacgcg gccacatagt ctgtggcgct ccacacgagc caaccaagcg tcagcaccgc
     3001 ggcgagctgc aggggccagg ccagacgcca gatctggccc gggagcacga cgttatcgcc
     3061 gaggaatgtg gcgatctggg gtacgtagac gattacggtc gtcaagtaca tcattccgaa
     3121 gagcagttgt gcggccaggc tccgcttcag gcgccagagc aagaaaggaa gcccgatcag
     3181 gaa
//
LOCUS       DATMJA010000194         3036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_84525, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000194 DATMJA010000000
VERSION     DATMJA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84525"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VNT12_04585"
     CDS             <1..701
                     /locus_tag="VNT12_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWI45429.1"
                     /translation="ELVAAEEEISREELDDITALRYEQYQRSLENDREFQKRYMVPVT
                     IPQRRKDSIVVDEDSGVFPTTREGLAKLEPVAEGGVVTFGSQTHPADGTAGMILTGEE
                     KARGLADGDGIVRLLATGFARVGKARMPKAPVPAAEMALADAGIPISGVDVIKTHNPF
                     AVNDAYFARQMGVQVKDMNNYGSSLIFGHPQGPTGARLIAEMIEELRLRGGGIGLFTG
                     CAAGDSGAALVVLV"
     gene            724..1491
                     /locus_tag="VNT12_04590"
     CDS             724..1491
                     /locus_tag="VNT12_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HWI45430.1"
                     /translation="MHVEESTFFITGGGSGLGAATARLLVENGAHVALADVDEEAGEQ
                     MASQIGSGTKFVRTDVTDEESVQDALGSVVETFGSLNGAVNCAGIGPAAKVVGKKGVH
                     DLGLFTKTVEINLVGTFNVIRLAAVRLAENEPDNEGERGVIINTASVAAYDGQIGQSA
                     YAASKGGVVALTLPVARELASSGIRVVTIAPGIFETPMLAALPEDAKESLGKQIPFPP
                     RLGRPEEYAALAKHIVENQMLNGEVIRLDGGIRMAPR"
     gene            1496..2674
                     /locus_tag="VNT12_04595"
     CDS             1496..2674
                     /locus_tag="VNT12_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI45431.1"
                     /translation="MSDARSTLHPDLFGLDGLLTEEEREVRERVRAFSDAEVIPIMAD
                     CWERGEFPFQLVPKLAALGICGDTIRGYGCAGLSAVGAGLAAMELARGDGSIYTFFGA
                     TSGFAINTIANLGSEEQKEKWIPGMARLEKLGTFALTEPEHGSDAVMLETKARREGDE
                     YVIDGKKRWIGNGTVADVIIIWARDENDDLGGFLVEKGSPGLQATRITGKASKRPGIQ
                     ADLSLEGVRVPVENRLPGARTFKDATSALAVVRCGVAWEALGHAIAVYETALAYTRER
                     KQFGKLIASFQLVQDKLAKMLADITTMFLLCIQLGRRAEAGKMTDVAASLAKMNNAQK
                     ARRICLDARDIMGGNGILYEYHVARHWADMEAVYTYEGTDSINSLIVGREITGHSAFL
                     "
     gene            complement(2822..>3036)
                     /locus_tag="VNT12_04600"
     CDS             complement(2822..>3036)
                     /locus_tag="VNT12_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45432.1"
                     /translation="YFWWVNTPDGGGTPHFSARGKYGQYIYVAPEKDLVIVRLGKEEG
                     ERGYGYWISLFEELATGLDPSVKESR"
BASE COUNT          611 a          933 c          985 g          507 t
ORIGIN      
        1 gtgagctcgt cgccgccgag gaagagataa gccgcgagga gctcgacgac atcaccgcgc
       61 tgcgctacga gcagtatcag aggtcgctag agaatgaccg cgagtttcag aagaggtaca
      121 tggtccccgt caccatcccg cagaggcgca aggactcaat cgtcgtggac gaagactcgg
      181 gcgtcttccc cacgaccagg gaaggcctcg cgaagctcga gcccgtcgca gaaggtggcg
      241 tcgtcacctt cggttcccag acgcaccccg cggacgggac agcgggcatg atcctcacag
      301 gtgaggagaa ggcacgcggg ctcgcggacg gagatggcat cgtcaggctg cttgccacgg
      361 ggttcgcgcg ggtcggcaag gccaggatgc ccaaggcccc ggtccctgcg gccgagatgg
      421 ccctcgccga cgccggcatc cccatctcag gcgttgacgt cataaaaacg cacaacccgt
      481 tcgccgtcaa cgacgcctac ttcgccaggc agatgggggt gcaggtcaaa gacatgaaca
      541 actacgggtc cagcctcatc ttcggccacc ctcagggacc gacaggagcg cgccttatcg
      601 cggagatgat cgaggagctc aggctacggg gcggcgggat cggcctcttc accggctgcg
      661 ccgccggaga ctccggcgcc gccctcgtcg tgctggtcta gtggaccaca gggaggagaa
      721 gctttgcatg tagaggagag cactttcttt attacaggtg gaggctcggg cctcggggcg
      781 gcgacggccc gtctcctcgt agagaacggg gcgcacgtcg ccctcgccga cgtcgacgaa
      841 gaggccggtg agcagatggc ttcgcagatc gggagcggca ccaagtttgt ccgtaccgac
      901 gtcacggacg aagagagcgt gcaggacgcg cttggttcgg tggtggagac cttcggttcc
      961 ttgaacgggg ccgtgaactg cgccgggatc gggccggctg ccaaggtcgt cgggaagaag
     1021 ggcgtccacg acctggggct cttcacgaag acggtcgaga tcaacctcgt aggcaccttc
     1081 aatgtcatcc gtctcgcagc cgtcaggctg gccgagaacg agccggacaa tgaaggcgag
     1141 cgcggggtca tcatcaatac cgcgagcgtc gctgcctatg acggccagat cgggcagtcc
     1201 gcctacgccg cttcgaaggg cggcgtcgta gcgctcacgc tccctgtggc ccgcgaactc
     1261 gcctctagcg gtatccgggt ggtaacgata gcccccggca tcttcgagac gccgatgctc
     1321 gcggcgctcc ccgaggacgc gaaggagtct cttggcaagc agataccttt ccctccgagg
     1381 ctcggcaggc cggaagagta cgcggccctg gcgaagcaca tcgtcgagaa ccagatgctc
     1441 aacggcgagg taatacgtct cgacggaggc atcaggatgg cgcccaggtg aatccgtgag
     1501 cgatgcgcgc tccaccttgc atccggacct cttcgggctc gacggtctgc taaccgagga
     1561 ggaacgcgag gttcgcgagc gcgtacgggc cttctcggac gctgaggtca tcccgatcat
     1621 ggccgactgc tgggagcggg gagaattccc gttccagctg gtcccgaagc tggccgcgct
     1681 cggtatctgt ggcgacacca taagaggata cggttgcgcg ggtctcagcg cggtcggggc
     1741 tgggctcgcg gccatggagc tggcgcgggg cgatgggagt atctacacgt tcttcggggc
     1801 gacttcaggc ttcgccataa acacgatcgc caacctcggc agcgaggagc aaaaggagaa
     1861 gtggattcca ggcatggccc ggctggagaa gctcgggacc ttcgctctga ccgaaccgga
     1921 gcacggatca gatgccgtga tgctcgagac gaaggcccgc agggagggcg acgagtacgt
     1981 tatagatggc aagaaacgct ggatcggaaa cggcacggtg gccgatgtga tcatcatctg
     2041 ggcccgcgac gagaatgacg acctcggagg attcctcgtc gagaaggggt cgcctggtct
     2101 ccaggcgact aggatcacgg gcaaggcctc caagagacca ggcattcagg cagacctttc
     2161 gctggagggc gtcagggtgc ctgtagagaa ccggctgccc ggtgcgagga ccttcaagga
     2221 cgccaccagc gcccttgccg tcgtcaggtg tggcgtcgcg tgggaggcac tcggacacgc
     2281 cattgcagtg tatgagacgg cgcttgccta caccagagaa cgcaagcagt ttggcaagct
     2341 cattgcatcc ttccaactcg tgcaggataa gctcgcgaag atgctcgccg acatcaccac
     2401 gatgttcctt ctctgcatac agctcggcag gcgcgccgaa gcaggaaaga tgacggacgt
     2461 tgccgcgtcg cttgccaaga tgaacaatgc ccaaaaagcc cgaaggatct gcctcgacgc
     2521 ccgcgacatc atgggcggca acggcatact ctacgagtac cacgtcgccc gccactgggc
     2581 tgatatggag gccgtctaca cctatgaggg caccgactcc atcaactcct tgatagtggg
     2641 gcgcgagatc accggccaca gtgcgttctt ataaggaaga tcggaacctg gcattcccca
     2701 accgaagtgg aaagccgatg aacgccgaca tcctctacca tcgtggcttc aagccgctcc
     2761 tcgagagggc ggggctctca agcttcgctt tccactctct gcctcatccg ttcttgaggc
     2821 actatcgcga ttccttcacc gagggatcga gccctgtggc caactcctca aagagagaga
     2881 tccagtagcc ataacctcgc tctccttcct ccttgccgag ccggacgatc accaggtcct
     2941 tctcaggggc gacgtagatg tactgcccat acttgccgcg cgccgagaag tggggggtcc
     3001 ctccgccgtc gggcgtgttg acccaccaga agtact
//
LOCUS       DATMJA010000195         5968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_380974, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000195 DATMJA010000000
VERSION     DATMJA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380974"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VNT12_04605"
     CDS             complement(<1..239)
                     /locus_tag="VNT12_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI45433.1"
                     /translation="MRPEEIDGLTVGGLLDLVAERRPDDEALVYADRNLRYTYMEFKA
                     VVEKCARSLMALGIYKGDHVAVWGQNVPEWVILQFA"
     gene            complement(322..1266)
                     /locus_tag="VNT12_04610"
     CDS             complement(322..1266)
                     /locus_tag="VNT12_04610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HWI45434.1"
                     /translation="MSSDIATERAALIVNTRSRTGEKTFFKALDRLQELGVNLGVTYA
                     IRDPARLPETVREVLHDGSGYRFLILGGGDGSVSSVVDFMAHHDTVLGLLPLGTANDF
                     ARTLEIPQDIEGACNTIADGKVVDVDLGLAGDNYYVNVASVGLGVEATLALSPWLKKS
                     TGPLAYPAAAIRAFLRHEPFSARLTFPDGDHEPVEYDRLLQIAVGNGRFYGGGMIVAP
                     ESGIDDKLLDIYVIDLGRRRNLIGVARYLKSGDFIRMEGVHHFRTSRVQIETDPELAV
                     NIDGEVVARTPQDFSVVQNALHVLVPQESDAARHDNDE"
     gene            complement(1522..2322)
                     /gene="iolB"
                     /locus_tag="VNT12_04615"
     CDS             complement(1522..2322)
                     /gene="iolB"
                     /locus_tag="VNT12_04615"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HWI45435.1"
                     /translation="MPDLLAISEDQPDRDGTLIKVTPESAGWEYVGFEVLRLEAGRSV
                     SRHTGRDEVCLVPLSGTCTVSAGDEEWKDIGGRESVFEGPPYAVYLPPGTEYTLQAST
                     ELEIAVASAPAERGVDPMVVRPGDIDVEIRGSGNMEREIRPILMEDRGAERLLVVEVL
                     TPNGHWSSYPPHKHDHDDPPRERYLEETYYHKIRPEKGFAVQRVYTEDGSLDETLTFH
                     DGDVVLVPKGYHVVSTPPGYDLYYLNVMAGPVREWRIKNHPDHEWLLG"
     gene            complement(2338..4251)
                     /gene="iolD"
                     /locus_tag="VNT12_04620"
     CDS             complement(2338..4251)
                     /gene="iolD"
                     /locus_tag="VNT12_04620"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HWI45436.1"
                     /translation="MYYKSSNGGDLLSTVRLTAAQALVRFLTEQYVERDGIERRFFAG
                     CFGIFGHGNVAGVGQALQQYSDGLPYYQARNEQAMVHTASAYARQKNRLQAFACTTSI
                     GPGATNMVTGAALATINRLPVLLLPGDVFASRGPDPVLQQLETPHDGTLSVNDCFRPV
                     SRYWDRVVRPEQVIPVALAAMRVLTDQAETGAVTLALPQDVQTEAFDYPEAFFEKRVW
                     HIGRPLPDAGAMERASTMIRESSRPMIVAGGGVIYSEATEALRRLCERTGTPVGETQA
                     GKGSLPHDHPCALGAVGVTGTFAANRAAREADLVIGVGTRWSDFTTASKTAFQNPEAS
                     FVNINIAGFDATKHAGLALVGDARSTLQALDDVLSGYEVDRAYRERNAGESREWDGEV
                     GRLYSLGHEPLPAQCEVIGAVNSFSDPEDVVVCAAGSMPGDLHKLWRTRDPKGCHVEY
                     GYSCMGYEISGGLGVKMAAPEREVYVMCGDGSYLMMNSEIVTSIQEGYKLTIVLVDNS
                     GFSSIGALSRSVGSDGFGTHYRYRKGGSIGLDSEEAPGDVLPVDLAKGAESLGAYVIR
                     TENIEGLKKALADAKDIDRTVVIHIPVDRYEGVPDYDSFWDVPVSEVSEMESVTSARE
                     EYAENKKAERRYL"
     gene            complement(4348..5271)
                     /gene="iolC"
                     /locus_tag="VNT12_04625"
     CDS             complement(4348..5271)
                     /gene="iolC"
                     /locus_tag="VNT12_04625"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HWI45437.1"
                     /translation="MGRVSVDLYPEQIGVTLAEVETFAKSLGGSATNVAVAAARLGHP
                     SALITKAGDDGFGRYVRAALEGFGVDTTWVSTEPELRTPLAFCEIHPPDDFPILFYRE
                     PKAPDMNLTVEDLGFDAISAVPLFWITGTGLSDEPSRTATLAALEHRPTGITVFDLDY
                     RPTLWASQEEAAHCYREALRHATVAVGNQTEVEVAVGTGDPFEASEALLDLGIDLAIV
                     KRGPDGVLARTKDGVAEAPPIEVEIVNGLGAGDAFGGALCHALLSGWDAKRTIHYANA
                     AGAIVASRLACADDMPTREEVEQLLEKSSDA"
     gene            complement(5462..>5968)
                     /locus_tag="VNT12_04630"
     CDS             complement(5462..>5968)
                     /locus_tag="VNT12_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWI45438.1"
                     /translation="DDEWDELFATLAMVEEIGAEHGLSVVLHPHYGTEIENPEHVQRF
                     LDGCDTALCLDTGHSMVGGGDPVALTESAAARIRHVHLKDVDRDLAERVVSGTLPYEQ
                     AVRDGLYRPLGDGDVDVRRVLEVLGGAGYDGWYVLEQDIMLAADPGYGPAAEVGRSLA
                     YLKGVLGT"
BASE COUNT         1050 a         1899 c         1860 g         1159 t
ORIGIN      
        1 gcgaactgca ggatcaccca ctcaggcacg ttctggcccc acacggcgac gtggtcgccc
       61 ttgtatatac cgagcgccat caaagaccgg gcgcacttct cgacgaccgc cttgaactcc
      121 atgtaggtgt aacgcaggtt ccggtcggcg tagaccaggg cttcatcgtc agggcgtcgc
      181 tcagctacca gatccagaag cccgcctacg gtcaaaccat cgatctcctc ggggcgcatt
      241 ctttcctcct cgtttcggtc cccaccgatg ttccgaagtc tagcaatcga tactgtccac
      301 gcaagcgcgt ctgtcgctgc cctactcgtc gttgtcgtga cgcgcggcgt cggactcctg
      361 cggcaccagc acgtggaggg cgttctggac cacggagaag tcttgcgggg tcctcgccac
      421 cacttccccg tctatgttga ccgcgagctc agggtcagtc tcgatctgga cgcgcgaggt
      481 cctgaagtga tgaacgcctt ccatcctaat gaaatcccca ctcttcaggt atctggcgac
      541 cccgatgagg ttcctccgcc ggccgagatc tatgacgtag atgtcgagga gcttgtcgtc
      601 tatacctgat tcgggggcga cgatcatacc cccgccgtag aagcgcccgt tgccgacggc
      661 gatctgaagg agacggtcgt attcgacagg ctcatggtca ccgtcgggga aagtcagcct
      721 ggcggagaag ggttcgtgcc tcaggaacgc cctgatggcg gccgcgggat aggccagcgg
      781 acccgtgctt ttcttcagcc acggggagag ggccagcgtg gcctcaaccc cgagtccgac
      841 ggaggccacg ttcacgtagt agttgtcgcc agcgagaccg aggtctacgt cgacgacctt
      901 gccatccgct atcgtattgc aggcgccctc gatgtcctgc ggtatctcca gcgtcctggc
      961 gaaatcgttg gccgtaccga ggggcaggag gccgagaacc gtgtcgtggt gcgccatgaa
     1021 gtcgaccacg gagctcacag acccgtcacc gccgccaagg atgaggaacc tgtaacccga
     1081 gccgtcgtgg aggacctcac gcaccgtctc ggggagccgc gcaggatccc ttatagcgta
     1141 ggtcacaccg agattgacgc caagctcctg aaggcggtcg agggccttga agaacgtctt
     1201 ctccccggtg cgggaacgcg tgttgactat caaggccgca cgctcggttg cgatgtcact
     1261 gctcatacgc aatccagaag atcggtagcg gtgtgatgcg cagagatccc gcctccgtgg
     1321 attctcgcgt tcgcgggaat gacgagggtg gaggctgcat ttcgcgctgt gcgcatttca
     1381 tgctcctggt actcgctcgt caccatgaca aagagagtat acgagtcccg tgccccaagg
     1441 gtttcgcggg gtggcgactc gaacgcgggg tcagggcgtc cgctccacct tcttcggttc
     1501 gtgttcctgt ctctcccttc tctacccgag cagccactcg tggtcgggat ggttctttat
     1561 cctccactca cgtaccggtc ctgccatgac gttgaggtag tagaggtcgt aacctggagg
     1621 cgtcgagacc acgtggtaac ccttcggcac gagcaccaca tcaccgtcat ggaacgttag
     1681 cgtctcgtcg agcgagccgt cttcggtata gaccctctgc acagcgaatc ccttctctgg
     1741 acggatctta tggtagtaag tctcctccag gtaccgctcg cgcggagggt catcgtggtc
     1801 gtgtttgtgc ggcgggtagc tcgaccagtg gccgttcggg gtcaggacct cgactacaag
     1861 gagccgctcg gccccgcggt cctccataag gatcggacgt atctcccgct ccatgttgcc
     1921 cgagccgcga atctccacat cgatgtcccc tggccttacc accatcgggt cgacgccccg
     1981 ctcggcaggc gccgaagcaa ccgcaatctc cagctctgtc gaagcttgta gcgtgtactc
     2041 cgttccaggt ggcagataga cagcataagg aggtccttcg aacacgctct ccctgccacc
     2101 gatgtccttc cactcctcat ctcccgccga cacggtgcac gtccccgaga gcggaaccag
     2161 gcacacctca tctctccctg tgtgccggct cacgctacga cccgcctcga gtctgagcac
     2221 ctcgaatccg acgtactccc agcctgcgga ctccggcgta accttgatca gggtgccgtc
     2281 gcggtcaggc tggtcttcgc tgatagcgag taggtctggc acgctgaaga ctcctttcta
     2341 gaggtatctg cgttctgctt tcttgttctc cgcgtactcc tcgcgggccg aggtcacgga
     2401 ctccatctcg gacacctcgg agacgggtac gtcccagaag gagtcgtaat cggggacgcc
     2461 ttcgtagcgg tcgacgggga tgtggatgac cacggtcctg tcaatgtctt tggcgtctgc
     2521 gagagctttc ttcaggcctt cgatgttctc ggtccggatg acataagcac cgaggctctc
     2581 tgcgcctttg gccaggtcga cgggcaggac gtcgccgggc gcctcctccg agtcgaggcc
     2641 gatggaccct cccttgcggt agcggtagtg ggtgccgaag ccgtccgagc cgacggagcg
     2701 cgagagcgcc ccgatagagg aaaacccgga gttgtcgacc aggactatgg tgagcttgta
     2761 gccttcctgg atggaggtta cgatctcgga gttcatcatc aggtaagaac cgtcgccgca
     2821 cattacgtag acctcgcgct cgggggccgc catcttcacg ccgagcccgc cggagatctc
     2881 ataccccatg cacgagtagc cgtactcgac gtggcagccc ttcgggtcgc gcgtcctcca
     2941 gagcttgtgc agatcccctg gcatggaacc cgccgcgcag acgactacgt cctcgggatc
     3001 ggagaacgag ttgacagccc ctatgacctc gcactgggcc ggcagcggct cgtgacccag
     3061 ggagtacaac ctaccgactt ccccgtccca ctcccgcgac tcgccggcgt tccgctccct
     3121 gtaggctcga tccacctcat agcctgagag aacatcgtcc agcgcttgga gcgtagatcg
     3181 ggcgtcaccg acgagggcga ggccggcgtg tttggtggcg tcgaagccgg cgatgttaat
     3241 gttgacgaag cttgcctcgg ggttctggaa cgccgtcttg gaggccgtgg tgaagtctga
     3301 ccagcgggtg cccaccccga tgacgagatc tgcctcgcgg gccgcgcggt tcgccgcgaa
     3361 ggtgcccgtc acgccgacgg cgccgagggc gcaagggtgg tcgtggggca gggagccctt
     3421 gccagcctgc gtctcgccga caggggttcc ggtgcgttcg cacaggcgcc gcagggcttc
     3481 cgtcgcctcc gagtagatga cgcccccgcc ggccacgatc atgggccggc tggactcccg
     3541 gatcatggtc gaagcacgtt ccatcgcgcc ggcatcgggc aaagggcgtc caatgtgcca
     3601 gactcgcttc tcgaagaacg cttcaggata gtcgaaggcc tccgtctgca cgtcctgcgg
     3661 gagcgcgagg gtaacggccc ccgtctcggc ctggtctgtg agcacgcgca tcgcggccag
     3721 ggcgacaggt atcacctgtt cggggcgcac taccctatcc cagtaccgag ataccggcct
     3781 gaaacagtcg ttgaccgaga gggttccgtc gtggggggtc tcaagttgct gtagaacggg
     3841 gtcgggtcca cgcgaggcga agacgtcgcc aggcagtagc agaacgggca gacggttgat
     3901 cgtggcgagg gccgctcccg taaccatgtt ggtggcgccg ggcccgatgg atgtcgtgca
     3961 ggcgaaggcc tggaggcgat ttttctggcg ggcgtaggcc gaggccgtgt gtaccatcgc
     4021 ctgctcgttg cgggcctggt agtaagggag gccgtcggag tactgctgga gcgcctggcc
     4081 gacaccggcg acgttaccat ggccgaagat accgaagcat ccggcgaaga agcggcgttc
     4141 gatcccatct ctctctacgt actgctcagt caggaagcgc accagcgcct gggccgccgt
     4201 caaccttacc gtggacaaga gatcaccccc gtttgaagac ttgtaataca taatgtggtt
     4261 gatcgctcct gatgggaggt gcgcagctcc tgccgtagtg gattcccgcc tacgcggtaa
     4321 cgacgagaaa gaaggcggaa acgactctca ggcatctgag gacttctcca gcagttgctc
     4381 cacctcttcg cgggtgggca tgtcgtcggc gcacgccagg cgagaggcga cgatggctcc
     4441 cgcggcgttc gcgtagtgga tggtcctctt ggcgtcccac cccgagagaa gcgcgtggca
     4501 gagcgctcct ccgaaggcgt cacctgcgcc gagaccgttg acgatctcta cctcaatcgg
     4561 cggggcttcg gctactccgt ctttcgtgcg ggcgagcacg ccatcaggtc cccgcttgac
     4621 gatcgcaaga tctatgccaa ggtcgagtag cgcttcggaa gcctcgaagg gatctcctgt
     4681 gccaacggct acctcgacct cggtctggtt acctacggcc actgtggcgt gccgcagtgc
     4741 ctccctgtag caatgggccg cctcctcttg cgaagcccag agtgtcggcc tgtagtcgag
     4801 gtcgaagacc gtgatgcccg tgggcctgtg ctctagggcc gcgagggtcg ccgtacgact
     4861 cggctcgtcg gagagaccgg taccggtgat ccagaacaac ggcacggccg agatcgcgtc
     4921 gaagccgagg tcttcgaccg tcaggttcat atcaggggcc tttggctcgc ggtagaacag
     4981 gatcggaaag tcgtcgggag ggtggatctc acagaatgcc aggggagtcc gcaactccgg
     5041 ttccgtactg acccaggtgg tgtccacacc gaacccttcc agcgcggcgc ggacgtatcg
     5101 gccgaagccg tcgtcgccgg ccttggttat gagggcggag gggtggccga gacgggcggc
     5161 ggcgacggcc acgttcgttg cgctgccgcc gagagacttg gcgaaggtct cgacctcggc
     5221 gagcgtaacc ccgatctgct caggatacag gtccacgctc actcttccca tcgtgatgac
     5281 ctcgggattc atacgtaatc cagcagatcg cttgtggttg gaatagcgga gattccaccg
     5341 cagtggattc ccgcttgcgt gggaatgaca gttggtagga cgctcaaggt aggagaaaag
     5401 cagtacgtgt tgcgctcgcg cttcttgtat gctgcagaaa agtgatcctg cggacttcgt
     5461 atcacgtccc gagaactccc ttcaagtagg cgaggctcct gcccacctca gcggcaggac
     5521 cgtagccagg atcggcggcc agcattatgt cctgttccag cacgtaccat ccatcgtagc
     5581 ctgccccgcc cagtacttcc agcacgcgcc tgacgtccac gtcgccgtct cccagcggcc
     5641 tatagagccc gtccctgacc gcctgttcat agggcagcgt gcccgagacc acccgttcgg
     5701 cgaggtcccg atccacatcc ttcaagtgga cgtgacgaat cctcgccgct gccgactccg
     5761 tcagcgcgac gggatccccg cccccgacca tagaatgtcc cgtgtcgagg cagagtgctg
     5821 tgtcgcaacc gtcgaggaaa cgctgcacgt gttcggggtt ttcgatctcg gttccgtagt
     5881 gtgggtggag caccacgctg aggccgtgct ctgccccgat ctcctcgacc atcgcaagcg
     5941 tggcgaacag ctcgtcccac tcgtcgtc
//
LOCUS       DATMJA010000196         3975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_440412, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000196 DATMJA010000000
VERSION     DATMJA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440412"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VNT12_04635"
     CDS             <1..186
                     /locus_tag="VNT12_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45439.1"
                     /translation="ILEAMRRLALRVRLVAEPSGAVTFAAYLFHREELPTSRLTVAVI
                     SGGNVEPGLLAEVLSTR"
     gene            complement(349..2079)
                     /locus_tag="VNT12_04640"
     CDS             complement(349..2079)
                     /locus_tag="VNT12_04640"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWI45440.1"
                     /translation="MRMSTLFGLTLREAPSGVEVDGHGLLLRAGYIRQLAAGIFSYLP
                     LAKRSIGKIENILREEMDAAGGQEVSMPVVQPADVWERSGRYSTTGQELVRLTDRRNR
                     GMVLAMTHEEIVAGLAASEVDSYQRLPRLVYQIQTKFRDDPRPRAGLIRAREFTMKDA
                     YSLDRDERGLDQQYSTLYKAYFKIFARCGIPAIAVGADVGIMGGSMAHEFMYLTPIGE
                     DTILICDNCAYTANRQVARYRKPIPEAEEQRPIEKVATPGAGTIESLARSLKISESRT
                     AKALFQVAAMEGSERFVLAVVRGDMELNETKLANAVGASGLRPALPEEVRAIGAEPGY
                     GSPLGVGGAIVAVDDAIPASQNLVAGANEEGFHFLNVNYGRDFEADIVADIASAVDGS
                     LCTECGDAMRAVRGVEVGNIFKLGTSYSEEFGASFLDADGRRKPVVMGSYGIGLGRLL
                     ACIAEEHHDENGLIWPVSIAPYHVHFVAAEAGEMADGLYERLVSAGVEVLYDDRRESL
                     GAKFKDADLIGAPIRLTLTPRSLQRGGVEIKARCGSEGYVVSIEGAVAVVRRAISDLE
                     AEISPDDPVT"
     gene            complement(2220..3113)
                     /locus_tag="VNT12_04645"
     CDS             complement(2220..3113)
                     /locus_tag="VNT12_04645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI45441.1"
                     /translation="MIETDCGVRVRQMGDADLQRILELRSIVRWSADPRAFELLRGVR
                     DARWAVADAPDGDLAGMVGAVPLGDIGVLCHLAVHDGYRGLGLGALLSSWAVGYLRSR
                     GAKTIRLYSTLRAEDLYRSLGFKEVTPRTVYRIEEGLWRLRVPDQVNGHRVETLTFGD
                     LPELYGVDRWSYGADRSALLFATLRLHPGRGLVARDSTGLIMGYLIRSASGSATRIGP
                     FLASTPDVARLLLARTLSATGGTPVQVTVPGPAGCHAHSLLQEFGFEGTEDRMRMELG
                     EKITACRTGLVHYGTTPYLAT"
     gene            complement(3430..>3975)
                     /locus_tag="VNT12_04650"
     CDS             complement(3430..>3975)
                     /locus_tag="VNT12_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI45442.1"
                     /translation="RQGKVLYVGVSEWTAEQISDAMRIAKEMNLDRIVSNQPQYNMIQ
                     RRIEVGVIPLCEREGVGQVVFSPLAQGVLTGKYRPGDAPGEGTRAADPASNRFMQQLM
                     NEEVLSAVDGLRSVASEAGLSLPQLALAWVLRQENVSSAIIGASRPEQVEDNAAASGV
                     KLPADVVSEIDGILDGVIQRG"
BASE COUNT          699 a         1309 c         1223 g          744 t
ORIGIN      
        1 atcctggaag ctatgcgccg cctggcgctg cgggtcaggc tggtcgcaga gccgagcggg
       61 gctgtgacgt tcgcggccta cctgttccac agagaagaac tgcccacttc tcgcctcacc
      121 gtggccgtga tcagcggcgg caacgtcgaa ccaggcctcc tcgccgaggt cctctccacg
      181 agataaggtc ggggaagccg ttcagcgcgg cgtggtctcg acagggtcac cgacgtggac
      241 agtgccgagc gtgtcgtgga tcaggttgcg tccgaagact gtcccaagct cggactttct
      301 gtaagtcgcg agcgtacgca gcggccgtaa cgtcggcagc tcaggccctc acgtaacagg
      361 atcgtcagga gagatctcgg cttcaagatc agagatcgcc ctcctgacca cggcgacggc
      421 cccttcgatg gagactacgt agccctcgga accgcagcgg gctttgatct cgacccctcc
      481 cctttgcagg gaacggggtg tcagggtcag gcgtatgggc gccccgatca ggtcggcgtc
      541 cttgaacttg gcccccaggc tctctcgtcg atcatcgtac aacacctcga cgcctgcgga
      601 gaccaacctc tcgtacagac cgtcggccat ctcacccgcc tcggccgcga cgaagtggac
      661 gtggtaaggc gcgatggaga cgggccagat gaggccgttt tcgtcgtggt gttcctcagc
      721 gatgcaggcc agtagccgcc ctagcccgat gccgtaagat cccatcacca cgggctttcg
      781 ccgtccgtct gcgtccagaa agctcgcgcc gaactcctcg ctgtagctcg tgcccagctt
      841 gaagatgttc ccgacctcga caccccgcac ggcgcgcatg gcgtcgccgc actcggtaca
      901 gagcgaacca tccaccgccg aggctatgtc ggcaacgata tcagcctcga agtcccttcc
      961 gtagttaacg ttcaggaagt ggaaaccctc ctcgttggca cccgcgacca ggttttggga
     1021 ggccggtatc gcatcgtcaa cggccacgat cgcccccccc accccgagcg gagagccgta
     1081 cccaggttcg gccccgatgg ccctgacctc ctcagggagc gcaggcctca gcccgctcgc
     1141 cccgactgcg ttcgccagtt tggtctcgtt tagctccatg tcgccacgga cgacagcgag
     1201 gacgaaccgc tcggagcctt ccatcgcggc gacctggaag agcgcctttg ccgtgcggga
     1261 ctcagatatc ttcagggagc gggccagcga ctcgatcgtg cctgcaccgg gtgtggcgac
     1321 cttctctatt ggtctctgtt cctcggcttc agggataggc ttgcggtacc tcgccacctg
     1381 gcggtttgcg gtgtaagcgc aattgtcgca gatgaggatg gtgtcctccc cgatgggcgt
     1441 aaggtacatg aactcgtggg ccatgctccc gcccatgatg cccacgtcgg ccccgacggc
     1501 tatggcgggt attccgcacc gggcgaagat cttgaagtat gccttgtaga gcgtgctgta
     1561 ttgttggtcg aggcccctct cgtccctgtc gaggctgtat gcgtccttca tggtgaactc
     1621 gcgagcccgg atcaggcccg ccctcggccg cgggtcgtct cggaacttgg tctggatctg
     1681 gtacacgagc ctgggcagcc gctggtaaga gtccacctcg ctcgcagcaa ggccggcgac
     1741 gatctcctcg tgggtcatcg ccagaaccat ccccctgttc ctgcggtcgg tgagcctgac
     1801 cagctcctgt cccgtcgtac tgtaccggcc ggatctctcc cacacatcgg ccggctgtac
     1861 gacgggcatg gagacctcct gcccaccagc cgcatccatc tcctctcgca agatgttctc
     1921 gatcttgccg atagaccgct ttgccagagg caggtacgaa aagatccccg ctgccagttg
     1981 cctgatgtac ccggccctga gtagcagccc gtggccgtca acctccaccc cggaaggcgc
     2041 ctcacgcaac gtcaggccga agagtgtact catgcgcatg gtagctatca gctcctcagc
     2101 tatcagccaa cgatgagcgg cggcgcatag agcgccagag tctgtttccg tttacaatac
     2161 cccacacaac tactcctttc tcgaaacgac gctttcgcgc cggaacaacc tcctgaaagc
     2221 taagtagcta gatacggcgt cgtaccgtag tggacgagcc cggtacggca ggcggttatc
     2281 ttctcgccca gttccatccg catccggtcc tccgtgccct cgaagccgaa ctcttgcaag
     2341 agggagtgtg cgtggcaccc ggcaggaccg gggacagtga cctggacggg cgtacccccc
     2401 gtcgccgaga gagtgcgggc gagcaggagg cgggccacgt cgggcgtaga agccaggaag
     2461 gggccgatgc gggtcgcgct gccactcgcg ctccggatca ggtagcccat gataaggccc
     2521 gtcgagtcgc gggcgacgag gccgcgtcct ggatgtagtc tcagggtggc gaagagtagg
     2581 gctgaacggt cggccccgta gctccagcgg tcgacgccgt agagctcggg caggtctccg
     2641 aacgtgagcg tctcgaccct gtgcccgttt acttgatcgg gaacgcggag tctccaaagt
     2701 ccttcctcga tccggtaaac ggtacgcggc gtgacctctt tgaaacctag cgaccggtag
     2761 aggtcctccg cccgcagggt cgagtagagc ctgatcgtct tcgcgccacg gcttctgagg
     2821 tagcctacgg cccaggagga gagcagggcg ccgaggccga ggcccctgta cccgtcgtgg
     2881 actgcgaggt ggcagaggac gccgatgtct ccgagcggca cagcaccgac catacccgcc
     2941 aggtcgccgt caggcgcgtc ggccaccgcc cagcgggcgt cgcgcacgcc gcgcaggagc
     3001 tcgaaagccc tcgggtcagc cgaccagcgt acgatactcc gtagctcgag gatgcgctgc
     3061 aggtcggcgt cgcccatctg cctgacgcgg acgccgcagt cagtctcgat cactggccgt
     3121 aacaagttct gggcggcaat cttttctgct tcgttgtctt ctctcgcggt gaaggccagc
     3181 aagattttcc ctcgatctct ctttattcat ggatagagac tcttccgcgc aataggcttc
     3241 ggagaaacca taagaggcgc ggatgcctcg agctcgcccc tagagggacg gtcggggcag
     3301 ggacggtcga gggcgccgtc gcgtaaagct gctcgcagta aaccttatgt gatgccgttc
     3361 cacacccgca atgctacaac acggcacgct gacatggaag gattctgaga gaaggttcgc
     3421 tgaacggccc taaccgcgct gtatcacccc gtccaggatc ccgtctatct cggagaccac
     3481 gtctgcgggg agctttaccc ctgaggcagc ggcgttgtct tcgacctgct cgggcctgga
     3541 tgcgccgatg atcgcgctgc tcacgttttc ctggcgcaac acccacgcga gggccagctg
     3601 tggcaggctg aggcctgcct cggacgcgac ggagcgcaac ccatctacgg cagagagaac
     3661 ctcctcgttc atgagctgct gcataaagcg gttggatgcc gggtcggctg cgcgggtgcc
     3721 ctctccgggc gcgtcgccgg gacggtactt gcccgtgagt acgccctggg cgagcggtga
     3781 aaagaccacc tggccgaccc cttcgcgctc gcacaagggt atgaccccga cttcgatcct
     3841 gcgttggatc atgttgtact gcggctggtt ggagacgatc cgatccaggt tcatctcctt
     3901 agcaatcctc atggcgtcgg agatctgctc cgccgtccat tcggacacgc cgacgtacag
     3961 aaccttcccc tgtcg
//
LOCUS       DATMJA010000197         5956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_559189, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000197 DATMJA010000000
VERSION     DATMJA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559189"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VNT12_04655"
     CDS             <1..322
                     /locus_tag="VNT12_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HWI45443.1"
                     /translation="EDPNRPALIHGDAWCANVLARGDRITAFLDPAIYYADPEIELAF
                     ISLFDSFGRPFLDRYQEIRPIRDGFFEVRRDLYNLYPLLVHTYYFGGGYPGSVRNTLD
                     RFGV"
     gene            complement(577..1044)
                     /locus_tag="VNT12_04660"
     CDS             complement(577..1044)
                     /locus_tag="VNT12_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HWI45444.1"
                     /translation="MSVSRALGLGAISGLRSLSGPAFISRAASRGDLDLDRTVFAFLG
                     SPRVSKTLVLMELGELVADKLPATPSRTSLPPLLGRAASGALVGAAVFVSEGRRATIG
                     AALGSTAAIAAGFAGERLRALIVEKTGLPDLVVALAEDATVLLVGLRSLRNVR"
     gene            complement(1041..2117)
                     /locus_tag="VNT12_04665"
     CDS             complement(1041..2117)
                     /locus_tag="VNT12_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWI45445.1"
                     /translation="MDPKTIVVMEGDQTGQELLEEALRVLDPAVTGLGLDFVRYDLSL
                     ENRRATDNEVVHQAAAAMKETGFGIKAATVTPEEPGDVGSPNAILRTGIDGTVIVRTG
                     RRIPGVNPTSGVHSPISVVRMAVDDAYGAKEWREGEGLDEVAYRTEKISRRVCRGVSE
                     FAFIQARKMRAKVFGGPKYTVSPVYEGMLKEEMDRAAENSRDVRYDPQLIDATYALLL
                     TTYGEPLVIPTLNRDGDCISDLVMQMFGSIAGAESLLISLDEDENPETVMAEAPHGTA
                     PSLEGKNVANPMAMILAGAALLTFLHEEDASRVSRAIYEATFETVLDGVKTADLGGSA
                     GTTEFTDEIIRHIRTKLEVWSALA"
     gene            2289..2495
                     /locus_tag="VNT12_04670"
     CDS             2289..2495
                     /locus_tag="VNT12_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45446.1"
                     /translation="MGVFFVLVAVIVIAVIVSFIFGSRRGGTGGGSGAASGYYGSLGD
                     HGNNGDGGGWFDGGGGDGGGGGGD"
     gene            complement(2687..3646)
                     /locus_tag="VNT12_04675"
     CDS             complement(2687..3646)
                     /locus_tag="VNT12_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI45447.1"
                     /translation="MLFRQLECFLAVARLGNLSRAAEEMYLTQPTLTARLKALEEELG
                     DPLFVRTSRGMRVTEAGREFLPYAERIVGGFEEGKRRLEELRGASGGRLRIGASPGVG
                     TYALPGLLERFAVEHPRVSISVRTGHSEAILEMTLKEEVQLGLTRAMRHPEIESLPLY
                     EDELVLIVDPGHRFTRRGTASLTEIGEEQLIFFDHDSSYFEQTHALFRNAGIRELRTM
                     EVDNIEAAKRMVEHRLGVAFLPRTAVVRSVSAGNLVLISVEENPEMSRSIVALKRRDV
                     PATGPVAAFLEVADTMGEKRGGAPLSPTLATEFENLQLIDLFT"
     gene            3890..4099
                     /locus_tag="VNT12_04680"
     CDS             3890..4099
                     /locus_tag="VNT12_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45448.1"
                     /translation="MIPVQYRDPQTEEILERRYEESAPAIGTRVRIGFDEYRVLYRWR
                     CVPTSCIVYVHRVAREVRREARPAA"
     gene            4183..5787
                     /gene="aceB"
                     /locus_tag="VNT12_04685"
     CDS             4183..5787
                     /gene="aceB"
                     /locus_tag="VNT12_04685"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HWI45449.1"
                     /translation="MTDKTRTYAEGIEFTAEVPDEFAEILTPEAVAFVAKLSREYRGR
                     VEDLLTRRAERQERISAGEMPDFLAETREIREGEWKIAPVPDDLQDRRVELTGPPDRK
                     MTINALNSGASCWMADFEDANCPTWHNMLESQLNMKEAIERTIAFDDPKSGKHYELNE
                     DVAVILARPRGWHLFEKHILVDGKQVPGGLFDFGLYFFHNAQTLLDNGSGPYFYLPKM
                     ESHLEARLWNDVFELAQDQLGIPRGTIKATVLIETILATFEMHEILYELREHSAGLNC
                     GRWDYIFSYIKKFREHDMLLPDRNSVTMTVPFMRAYSLLTINTCHQRSAHAMGGMAAQ
                     IPRSDDPEWTEFANNKVREDKEREAKDGHDGTWVAHPGMVSLAKEAFDEYMPQPNQLD
                     KMPDVNPSAEDLLEKPEGNITMDGFRNNISVGVQYIAAWLAGRGAVPVFNLMEDSATA
                     EISRAQVWQWIHHQRGILEDGTEVTEDLFNETLEEELEKIKTDIVGPERWEQDEFGNA
                     AELFARISTDDEFVEFLTLPGYEYLD"
BASE COUNT         1192 a         1867 c         1805 g         1092 t
ORIGIN      
        1 cgaagacccc aaccgtccgg cgctcatcca cggcgacgcc tggtgcgcca acgtgctggc
       61 acgaggagat cgcataaccg ctttcctgga tcccgctatc tactacgctg accccgagat
      121 cgagctggcg ttcatctccc tgttcgactc ttttggccgt cccttcctcg atcgctacca
      181 ggagatccgg ccgatacgcg acggcttctt cgaggtgcgc cgtgacctgt acaacctcta
      241 cccgctgctg gtccacacct attacttcgg cggtggctat ccgggttcgg tgcggaacac
      301 gctggaccgg ttcggcgtgt gaacttcctc ttggccacgt acagggtggt caggtagcac
      361 ttgacgatgc agccgtcgaa acaattgtcg ataaggttgg cgatgccgcg gaaggggagc
      421 tcgcgggtgt cgtcgtttag acgcctgtgt ccggagtagg tgctcaaaac acgcaaatag
      481 ccgttcagga tagccgggac gaacgtcgtc gtagagcgac gccgcctcat cgaagtactt
      541 cctgagcccg tccctttctt tggccatggg cttgcgctaa cggacgttcc tcagcgagcg
      601 gagaccgacg agcaagacag tggcgtcctc cgcgagcgcg acgacgaggt cggggagacc
      661 ggtcttctcg acgatgagcg cacgtagccg ttcccccgcg aagcccgcgg caatggctgc
      721 ggtagaacct agcgccgccc cgatagtggc gcgacggcct tcggagacaa agacggctgc
      781 ccccaccagg gctcctgagg cagcacgccc gagcagggga ggtagggagg tacggctcgg
      841 tgtggcgggc aacttgtcag cgaccaactc acccaactcc atcaacacta gcgtcttcga
      901 gacgcgcggt gagccgagaa aggcgaagac tgtacgatct aggtcgagat cccccctgct
      961 cgccgcgcgg ctgatgaagg ccggaccgga gagggagcgc aggcccgaga tggcgccgag
     1021 accgagggcc ctagagacgc tcacgcgagt gcgctccaca cttcaagctt ggttctgatg
     1081 tggcgtatga tctcatcggt gaactcggtc gtgcccgccg acccgccaag gtcggccgtc
     1141 ttcacgccgt cgaggacggt ctcgaaggtc gcctcgtaga tggcgcgcga gacccggctc
     1201 gcatcctctt catggaggaa ggtaaggagc gcggctcctg ccaggatcat ggccatcggg
     1261 ttggctacgt tcttgccctc tagcgaagga gccgtgccgt gcggagcctc ggccatgacc
     1321 gtctcgggat tctcatcctc gtcgagcgag atcaggagcg actcggcccc cgctatggag
     1381 ccgaacatct gcatcactag gtccgagatg cagtcgccgt cacggttgag tgtagggatg
     1441 acgagcggct cgccgtaagt ggtcaagagc agcgcatagg tggcgtctat aagctggggg
     1501 tcgtagcgga cgtctcggct gttctccgcg gcgcggtcca tctcctcttt gagcatcccc
     1561 tcgtagacgg gggagacggt gtacttgggg ccgccgaaga ccttggcccg catcttgcgc
     1621 gcctggataa aggcgaactc cgatacgccg cggcataccc ggcgcgagat cttctcggtc
     1681 ctgtaggcga cctcgtcaag cccctcgcct tcgcgccact ccttcgcccc gtaagcgtca
     1741 tcgacggcca tacggaccac agagatcggg gagtgcacgc cgctagtcgg gttgacacca
     1801 ggaatgcgcc tccccgtacg cacgataacg gttccgtcta tccccgttct caggatcgca
     1861 ttgggcgagc ccacatcgcc cggttcctcc ggcgtaacgg tcgctgcctt gatgccgaat
     1921 ccagtctctt tcatcgccgc agcagcttgg tgcacgacct cattgtccgt ggcccgtcgg
     1981 ttctccagcg acaggtcata gcgcacgaaa tccagcccga gccctgtcac tgcagggtcg
     2041 agcacccgta aggcttcttc gagcagctct tgtcctgtct ggtcgccctc cataacaacg
     2101 atcgtcttcg ggtccactcg tcgcacctct gtgaatagta tctttgagtt tcccctagag
     2161 tatacgatcc acacgtacac gcattcgtgc tcccaaacac cctccacttg catctgtcgt
     2221 tataatagtg tcgtgcttcc cggtagttga agcctgaatc tggggaaacc cgtgaaggga
     2281 gagttcgggt gggagtgttc ttcgtacttg tggcggtaat cgtgatcgcc gtgatcgtaa
     2341 gcttcatttt cggctcccgt cgtggcggta caggcggcgg cagtggggcg gcgagcggct
     2401 actacggaag cctcggagac catggtaaca acggagatgg cggtggctgg ttcgacggtg
     2461 gtggcggcga tggtggtggg ggaggaggcg attagccgcg gcaccaccct gaccgcttcg
     2521 ccgaaacgcg attcgcagag gtgttgaccc accgcgaatc tagcattcag ccaaaagcca
     2581 aaacaaaaag ctgaaatgct gaaagcgccc gacagggcgc gtgctgacat gctgaagtgc
     2641 tggttagctg acatgcttgc aaagaggttg tgctgcttcg cagctctcag gtgaacaggt
     2701 ctatcagctg caagttctcg aactcggtag ccagggtggg tgagagcggg gcgccacccc
     2761 gcttctcgcc catcgtgtca gcgacttcga ggaaggcagc aacggggccg gtcgcaggga
     2821 cgtcacggcg cttgagagcg acgatcgagc ggctcatctc cggattctcc tctacagata
     2881 tgagcacgag gttgccggcc gagacggacc tgaccacggc tgtgcggggc agaaacgcga
     2941 cccccagcct gtgctcgacc atcctcttgg cggcctcgat gttgtcgacc tccatagtcc
     3001 tgagctccct tatgcccgcg ttcctgaaca gggcgtgcgt ctgctcgaag tagctcgagt
     3061 cgtggtcgaa gaagatgagc tgctcctcac cgatctcggt caagctcgcc gtccccctcc
     3121 tggtgaaccg gtgccctgga tctacgatga gcaccaattc gtcctcatag agcggcaggc
     3181 tctcgatctc ggggtggcgc atcgcccgcg tgagaccgag ctgcacctcc tccttgagcg
     3241 tcatctcgag gatcgcctca gaatgccccg tcctgacaga gatcgaaacc ctcggatgct
     3301 ccaccgcgaa cctctccaat agcccaggca acgcgtacgt cccaacgccg ggggaagccc
     3361 cgatcctgag ccgccccccc gaagcgcccc tgagctcctc caaccgccgc ttgccctcct
     3421 cgaacccgcc tacgatcctc tccgcgtaag gcaagaactc cctgccagcc tccgtgaccc
     3481 gcatcccacg gctcgtacgc acgaagagag ggtccccgag ctcctcctcg agcgccttga
     3541 ggcgcgccgt caacgtcggt tgcgtgagat acatctcctc agcagcccgg ctcaaattcc
     3601 ccagccgcgc aacggccaga aagcattcca attgtcgaaa aagcacgctg tctcccttcg
     3661 gtcagttata gctagtcagc atttcagcac gcgccctgtc gggcacttgt cagcacttca
     3721 gctagtcagt gctgatctgc tgacaagcct cctggcgaag cctagcggcg tgctgactag
     3781 ctcgcagata taagtaaaac ctatatctgc gatacacaca aacgattgga ataataccgg
     3841 ggagattggt atcttgcttt cacgacggcg aggttgagag gagagatcta tgattccggt
     3901 acagtacagg gacccgcaga cggaggagat tttggagcgc cgttacgagg agagcgctcc
     3961 ggccatcggg acgcgggtga ggataggttt cgacgagtac cgggttctct acaggtggcg
     4021 ttgtgtgccg acctcgtgca tcgtctacgt gcacagggtc gccagggagg tccgtaggga
     4081 ggccaggcct gcggcctgag gaggtctccg gttccgagcg ttccggggtg tggcttgcta
     4141 gagtagcctt gccctggaag cagtagagag gagccggagg tcatgacgga taagacgcgt
     4201 acctacgctg aggggatcga gttcacagcc gaggtccccg acgagttcgc cgagatactc
     4261 acgcccgagg cggtcgcttt cgtggcgaag ctctcgcggg agtacagggg gagggtagag
     4321 gacctcctca ccaggcgggc cgagcggcag gagaggatca gcgccggcga gatgcctgat
     4381 ttcctggccg aaacgcggga gatcagggag ggcgagtgga agatagcccc cgtccccgac
     4441 gatcttcagg accgtcgggt agagctgact ggacctccag accggaagat gacgatcaac
     4501 gctctgaact cgggggcgag ctgctggatg gcagacttcg aagacgccaa ctgcccgacc
     4561 tggcacaaca tgctggagag ccagctcaac atgaaagagg cgatcgaacg caccatcgcc
     4621 ttcgacgacc cgaagtcggg caagcactac gagttgaacg aagacgttgc ggttatcctg
     4681 gcgaggccgc gagggtggca cctcttcgag aagcacatcc tcgttgacgg gaaacaggtc
     4741 ccaggtggtc tcttcgactt cggcctgtac ttcttccaca acgcgcagac gctactggat
     4801 aatggttccg gaccttactt ctacctgccc aagatggaga gccacctcga ggccaggctc
     4861 tggaacgacg tcttcgagtt ggcccaggac cagctcggca taccgcgggg caccatcaag
     4921 gccacggtcc tcatcgagac gatactcgcc acgttcgaga tgcacgagat cctttacgag
     4981 cttcgcgagc actccgccgg cctcaactgc ggccggtggg actatatctt ctcctacatc
     5041 aagaagttcc gcgaacatga catgctcctc cccgaccgca actctgtgac gatgaccgtc
     5101 ccgttcatgc gcgcctactc gctgctcacc atcaacacct gccaccagcg cagcgcgcac
     5161 gcgatggggg ggatggcggc ccagatccca cgcagcgatg atcccgaatg gactgagttc
     5221 gccaacaaca aagtgcgaga ggacaaggag cgcgaggcca aggacggcca cgacgggacc
     5281 tgggtcgccc accccgggat ggtctctctc gccaaagagg ccttcgacga gtatatgccc
     5341 cagcctaacc agctcgacaa gatgcccgac gtcaacccct ccgcagagga cctgctggag
     5401 aagcccgaag gcaacatcac tatggacggc ttccgcaaca acatctccgt tggcgtgcag
     5461 tacatcgcgg cctggctcgc tgggcgcggg gccgttcccg tcttcaacct gatggaggac
     5521 tcggcgaccg cggagatcag ccgcgcccag gtgtggcagt ggatccacca ccagagaggt
     5581 atcctcgaag acggcaccga ggtgacggaa gacctcttca acgaaaccct cgaggaggaa
     5641 ctggagaaga tcaaaaccga catcgtcggc cccgaacgct gggaacaaga cgagttcggc
     5701 aacgccgccg aactcttcgc ccggatctcg accgacgacg agttcgtcga gttcctgacc
     5761 ctcccaggtt acgagtacct agactagaga ggagcaccca tcccaactat catgcaaacc
     5821 ccgaggaccg gtgccccagt cgccctgcca aggagcgttg gccccgtacc cataccccat
     5881 aacacgaaag gggcgctcgt tgcgcccggt tagcacatat gtgagcatat aagtgcataa
     5941 tgcagcgtat cggcac
//
LOCUS       DATMJA010000198         5645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_263377, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000198 DATMJA010000000
VERSION     DATMJA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263377"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VNT12_04690"
     CDS             <1..330
                     /locus_tag="VNT12_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWI45450.1"
                     /translation="LYHYIEHPSFDREVEIVRLRVPDAEERLARQVAAATEKMRTMDL
                     YKPPGVAETLDWTEALVALGAKELDELLVEATLGSVLKYREDQQRAANAGLAALVSGL
                     GGVGRDG"
     gene            323..1465
                     /locus_tag="VNT12_04695"
     CDS             323..1465
                     /locus_tag="VNT12_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWI45451.1"
                     /translation="MAEGEIQTPPMLDAAVAFGRVLRGAGLRVGTDRLVEFARAIEEM
                     DPSRRDDVYWSGRITLTSRPEDIEVYDRAFELFWETGGGAKPRPRTKIRLSVPQPDRS
                     VMPPKKTVEKSESGEEAVRLRYSPVEVLRHKDFALYSPEEFAELQRLLADLRISGALR
                     KSRRLEPAPRGRHDPRRTLRGAMRTGGEAARHRFRKARVQPRRVVLLCDVSGSMAPYS
                     RALLRFLHASVVAGERLEAFSIGTRLTRITRELTTRDPDRALRQAADAVQDISGGTRL
                     GDAIKEFVDRWGQRGMARGAVVVIFSDGWDRGDVSVLAEQMQRLARLAYRVVWVNPLK
                     AAPGYKPLAAGMAAALPHVDVFLSGHNFESLEELARAVAAATERER"
     gene            1527..1841
                     /locus_tag="VNT12_04700"
     CDS             1527..1841
                     /locus_tag="VNT12_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWI45452.1"
                     /translation="MNPVIDRVASWREEGKKAALATVVKVERSGPLGPGTSMAVSEDG
                     QVVGSVSGGCVEPAVYEEAMAAIEGGEPRLLSYGISDDEAFGVGLTCGGTIHIFVERV
                     DW"
     gene            1841..2659
                     /locus_tag="VNT12_04705"
     CDS             1841..2659
                     /locus_tag="VNT12_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HWI45453.1"
                     /translation="MADLLSRWHEALAEQSPVAVATVIQGPAGSVGGKILVSPEDHTG
                     TAGNDDLDRAIVEGARAMLEGGKTGTVHYGPRGQRRMEDVAVFIQSFAPPPRMYVFGA
                     IDFASAVARVGKFMGYRVIVCDARAVFATRERFPSADEIIVAWPDEFLKTAEVDGRSV
                     ITVLTHDPKFDVPVLKRALDTPAGYIGAMGSRRTHDNRTARLKEEGVTDEQLARISSP
                     IGLDLGARTPEETAIAIAAEIVALRTGHSGGRLAERSGPIHSAASDTRTRDGVK"
     gene            2739..3614
                     /locus_tag="VNT12_04710"
     CDS             2739..3614
                     /locus_tag="VNT12_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWI45454.1"
                     /translation="MQVPARFEYEVAGSVGEAVELLERYGDGARLIAGGHSLIPMMKL
                     RLAAPEVVVDISGLDSELRYIEDEGGALRIGALTRHREVLESPVVRERYALLADAERM
                     IADPLVRNMGTIGGDLANADPAEDLPAAFVALDSEVLVRGPNGERTVHIDDLYVGPYE
                     TSLDHGEVLTEVRVPRTPGGSAYSKVKRKTGDYAAAAIGVALGMGGEEIESIRIGLCG
                     VGSRTLRARGAEDALTGERPGSEAYRRAGEIAAEECDPIEDAKGSVEYKRDLVRVLLG
                     RTVARAVERASGREV"
     gene            3617..4096
                     /locus_tag="VNT12_04715"
     CDS             3617..4096
                     /locus_tag="VNT12_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWI45455.1"
                     /translation="MRIKVNVNGAMLEAEFEPRLLLVHFVRETLGLTGTHWGCDTSNC
                     GACTVLLDGEPVKSCTVLAVRADSHDVTTIEGLWSGTDLTPVQEGFHVEHGLQCGFCT
                     PGMIMTATEFLERNPDPTEAEIREAISGNLCRCTGYENIVKAVQWAARNGSAQEVGA"
     gene            4096..>5645
                     /locus_tag="VNT12_04720"
     CDS             4096..>5645
                     /locus_tag="VNT12_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWI45456.1"
                     /translation="MATVQGHTLGDRMPRKEDGRFIRGKGNYVDDVRLPQMLQSAILR
                     SPYAHARINSVDTSAALELSGVHAVITGEDLAEQGLAWMPTLAGDTQAVLATDKVRFQ
                     GQEVAFVVADDTYIAQDALELIDVDYEPLDPVIDSRKALDKDAPVIRDDKDGKDDNSI
                     FFWDAGDKEATGKAFEEAEVKVRQEMYYPRIHPAPMETCGAVADYDVSTGEMTVHLTS
                     QAPHAHRTLFAIVSGLPEHQIRIISPDIGGGFGNKVPIYPGYVCAAVASLVLGRPVKW
                     METRSENLMSTSFARDYHMVGELAATSEGKILGARSTILANHGAFDAHAQPGEQYPTG
                     FFPIFTSCYDVPAAYAEATGVYTNRAPGGIAYRCSFRVTEAIYLMERTMDVLADELGM
                     DPVELRRKNFVTKEQMPYISVMGWEYDGGDYEGALQEALRIAGYEALLEEQKQAREEG
                     RIMGIGLSTFTEIVGAGPGKKCHIAGIEMFDAAELRIHPTGKAILKLGVKSQGQGHET
                     TFAQIVAG"
BASE COUNT         1088 a         1756 c         1928 g          873 t
ORIGIN      
        1 ctctaccatt acatagagca ccccagcttc gaccgggagg tggagatcgt acgcctcagg
       61 gtccccgacg ccgaggagag gctggcccgc caggtagccg ctgcgacgga gaagatgcgc
      121 actatggacc tctacaaacc acctggcgta gccgagaccc tcgactggac cgaggcgctc
      181 gtcgccctcg gcgcgaaaga gctcgacgag ttgctcgtcg aggcgaccct gggctccgtc
      241 ctcaagtacc gtgaggacca gcagagggcc gccaacgccg gcctcgccgc gctcgtcagc
      301 gggttgggtg gggtggggag agatggctga gggagagata cagaccccac cgatgctcga
      361 tgcggcagtc gccttcgggc gcgtgctgag gggagccggc ttgcgcgtcg ggaccgaccg
      421 tctcgtggag ttcgcccgcg ccattgagga gatggacccc tcccgccgcg acgacgtgta
      481 ctggtcgggc aggatcacgc tgacctcccg ccccgaagat atagaggtat acgaccgcgc
      541 cttcgagctt ttctgggaga ccggaggcgg cgccaagcca aggccgcgta cgaagatacg
      601 cctctccgtg ccgcagcccg accgctccgt catgcccccg aagaagaccg tggagaagag
      661 cgagtctggc gaggaggccg tcaggctccg ctatagcccc gtcgaggtgc tgcgtcacaa
      721 agacttcgcc ctgtactcgc ccgaggagtt cgccgagctg cagcgtctgc tagcagacct
      781 caggatctcc ggggcgctca gaaagagtcg gcgcctggaa cccgctccgc gcggacgcca
      841 cgaccctcgc cgcacgctgc gaggcgcaat gcggaccggt ggtgaggccg caaggcaccg
      901 cttccgcaag gccagggtgc agccgcggcg cgtcgtgttg ctctgcgacg tctccggctc
      961 catggcccct tacagccgcg cgctcctgcg cttcttgcac gcgagcgtcg tcgcggggga
     1021 gcggctcgag gcgttctcca taggaacgcg gctcaccagg atcacacgcg agctaactac
     1081 ccgcgacccg gacagggcac tcaggcaggc ggcagatgcg gtacaggaca tctccggcgg
     1141 aacgcggttg ggggatgcga tcaaggagtt cgtcgaccgc tgggggcagc ggggcatggc
     1201 gcgcggcgcc gtcgtcgtga tcttctccga cggctgggac aggggagacg tgtccgtgct
     1261 ggccgagcag atgcagcgcc tggcccgcct ggcctacagg gtcgtctggg tcaaccccct
     1321 caaagccgcc ccaggctaca aaccgcttgc ggcaggaatg gcagcggcgc tcccgcacgt
     1381 agacgtcttc ctctcgggcc acaacttcga atccttggag gaactcgccc gagccgtcgc
     1441 cgctgcaaca gagcgtgaga gataaaatga aggcttaagc tcacaggtat cagcaaagac
     1501 ctgaaaccta aagaaggagg cactccttga acccagttat cgatagagtc gcaagctggc
     1561 gagaggaggg aaagaaggcc gccctcgcca cggtcgtaaa ggtcgagagg tctgggccac
     1621 tgggacccgg gacctcgatg gcagtctccg aggacgggca ggtcgtcggt tccgtgagcg
     1681 gggggtgcgt ggagcccgcg gtctacgagg aggcgatggc ggcgatagag ggcggcgaac
     1741 ccaggctcct ctcctacggc atctccgatg acgaggcatt cggggtcggt ctgacgtgcg
     1801 gtgggactat ccacatcttc gtcgagcgcg tggactggta atggcagatc tactctcccg
     1861 ctggcacgag gccctcgccg agcagtcgcc cgtggccgtg gcgaccgtca tacagggacc
     1921 ggcgggttcc gttgggggta agatcctggt ctcccctgag gatcacaccg ggacggcggg
     1981 caacgacgac ctcgacaggg ccatcgtcga aggggccagg gcgatgctcg aagggggaaa
     2041 gacgggtacc gtccactatg gcccgcgcgg ccagcgcagg atggaggacg ttgccgtctt
     2101 catccagtct ttcgccccgc cgccaaggat gtatgtcttc ggcgccatag acttcgcttc
     2161 ggccgtcgcg agggtcggca agttcatggg ttaccgtgtg atcgtctgcg atgcccgcgc
     2221 cgtcttcgcc acccgcgagc gcttcccctc ggcggacgag atcatagtcg cctggcccga
     2281 tgagttcctg aagacagccg aggtcgacgg ccgaagcgtc atcaccgtcc tgacccacga
     2341 ccccaagttc gacgtgcccg ttctgaagag agcgctcgat acgccagcag gctacatagg
     2401 ggcgatgggg agccgcagga cgcatgacaa caggacggcc cgcctcaagg aggagggcgt
     2461 tacagacgag caactcgcgc gcatcagcag tcccatcggc cttgatctag gggcgcgcac
     2521 acccgaggaa acggcgatag cgatcgcagc cgagatcgtc gcgctgagga ccgggcattc
     2581 tggcgggcgc ctcgccgaac gatccggccc catccactcg gctgcgtcag acaccaggac
     2641 cagggacgga gtaaaatagt ccgctaaggg gtatttgttc cggtcctctg gccggaaaaa
     2701 ggggaactcc aacgtcgttg gagagagagg agagaggcgt gcaggttccg gcgcggttcg
     2761 agtacgaggt cgctggcagc gtgggggaag cagtcgagct tctagagcgg tacggcgacg
     2821 gggcgcgcct gatcgccggc ggccacagcc tcatccctat gatgaagttg cggctcgccg
     2881 cgcccgaggt cgtcgtagac atcagcgggc tggatagcga gctgcgctac atcgaggacg
     2941 agggtggggc tttgaggatc ggggcgctca ccaggcaccg cgaggtactc gagtctccgg
     3001 tcgtacggga acggtacgcg ctactggccg acgcggagag gatgatcgcc gaccccctcg
     3061 tcaggaacat ggggacgatc gggggtgacc tcgccaatgc ggacccggca gaggatcttc
     3121 cggctgcctt cgtcgccctc gacagcgagg tcctggtccg aggccccaac ggtgagcgta
     3181 ccgtgcacat cgacgatctg tacgtcggcc cctacgagac ctccctcgat cacggcgagg
     3241 tcctgacgga ggtcagagtg ccgcgcactc ctggagggag cgcttactcc aaggtcaagc
     3301 ggaagaccgg ggactacgcc gctgctgcaa tcggcgtcgc ccttggcatg ggcggcgaag
     3361 agatagagag catccgtatc ggactgtgcg gcgtcggaag ccgaactctg cgggcgaggg
     3421 gtgcggaaga cgcgctcaca ggggaacgcc cggggtcaga ggcgtacagg agggcagggg
     3481 agatcgccgc cgaggagtgc gatcccatag aggacgcaaa gggctctgtg gagtacaagc
     3541 gcgacctggt cagggtactc ctcggacgta cggtggccag ggcggtcgag cgcgcgtccg
     3601 ggcgggaggt atagaggtga ggataaaggt aaacgtaaac ggcgcaatgc tggaggccga
     3661 gttcgagccg cgcctgttgc tcgtccattt cgtaagggag accctggggc ttacaggaac
     3721 ccactggggg tgtgacacct ccaactgcgg ggcctgcacg gtgctcctcg acggggaacc
     3781 cgtcaagagc tgtacggtgc tcgctgtcag ggccgactcc cacgatgtga cgaccatcga
     3841 ggggctgtgg agcgggacag atctcacccc cgttcaggag ggttttcacg tcgagcacgg
     3901 gctgcaatgc ggcttctgta caccgggcat gataatgacc gccacagaat tcctggagcg
     3961 caatcccgac ccgacggagg cggagatcag ggaggccatc tcgggcaacc tgtgccgctg
     4021 cacgggttac gagaacatcg tcaaggcggt ccagtgggct gcgagaaacg gcagtgcaca
     4081 ggaggttggt gcgtaatggc cacagtgcag ggacataccc tcggcgaccg gatgcctcgc
     4141 aaggaggacg ggcgctttat caggggcaag ggaaactacg tcgatgacgt cagactgccg
     4201 cagatgctgc agtcggccat cctgcgcagc ccttacgccc acgcgaggat caatagtgta
     4261 gacacaagcg ccgcgctgga gctctccggc gtccacgcgg taatcactgg cgaggacctc
     4321 gccgagcagg gcctcgcctg gatgcccacc ctcgccgggg acacccaggc tgtgctcgcc
     4381 accgacaagg tgcgcttcca ggggcaggag gtcgccttcg tagtggccga tgatacctat
     4441 atcgcgcagg acgccctcga gctcatcgac gttgactacg agccactcga ccccgttata
     4501 gattccagga aggcgctcga taaagatgct cccgtcatcc gcgacgacaa ggatggtaag
     4561 gacgacaact ccatcttctt ctgggacgca ggggacaagg aggccacagg caaggcgttc
     4621 gaggaggccg aggtaaaggt tcgccaggag atgtactacc cgcgcatcca ccctgccccg
     4681 atggagacct gcggggccgt cgccgattac gacgtctcga ccggcgagat gaccgtccac
     4741 ttgacctcgc aggccccgca cgcccacagg acgctcttcg ccatcgtctc gggactgcca
     4801 gaacaccaga tcaggataat ctcccccgac atcggcggcg gcttcgggaa caaggtcccc
     4861 atctaccctg gctacgtgtg cgccgccgtc gcctccctcg tgctcggccg gcccgtgaag
     4921 tggatggaga cgcgctcgga gaacctgatg agcacgagct tcgcgcgcga ctaccatatg
     4981 gtaggagagc tcgcggccac cagcgaagga aagatcctcg gcgcccgctc gaccatcctc
     5041 gcgaaccacg gggctttcga tgcccacgcc cagccggggg agcagtatcc tacgggcttc
     5101 ttcccgatct tcacgagctg ctacgacgtg cccgccgcct acgccgaggc gaccggggtc
     5161 tacaccaaca gggctccagg cggcatcgcc taccgctgct cgttcagggt gacggaggcc
     5221 atctacctca tggagcgcac tatggacgtg ctcgccgacg agctcgggat ggaccctgtg
     5281 gagctccggc gcaagaactt cgtcacaaag gagcagatgc cctacatctc cgtcatgggc
     5341 tgggagtacg acggcggcga ctacgagggc gcgttgcagg aggctttgcg gatcgccggt
     5401 tacgaggcgc ttctggaaga gcagaagcag gcgcgtgaag agggcaggat catgggcata
     5461 ggtctctcca cgttcacgga gatcgtgggg gcaggccctg gcaagaagtg ccacatagcg
     5521 ggtatcgaga tgttcgacgc cgcggagctc agaatccacc ccacgggtaa ggctatcctc
     5581 aagctcgggg tcaagtcgca ggggcagggc cacgagacga ccttcgccca gatcgtggcc
     5641 ggaga
//
LOCUS       DATMJA010000199         3461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_618846, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000199 DATMJA010000000
VERSION     DATMJA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618846"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VNT12_04725"
     CDS             complement(<1..300)
                     /locus_tag="VNT12_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45457.1"
                     /translation="MTLRLGRNTSLFIDLETGVTGLKGIARGLGWRLGKVPALTRGAV
                     RIPSARRKPARRERGSKTPVFFVTGVGKSGTSWLMRTLDGHPDILCKGEGRFFAAD"
     gene            complement(384..1472)
                     /locus_tag="VNT12_04730"
     CDS             complement(384..1472)
                     /locus_tag="VNT12_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI45458.1"
                     /translation="MNLEAGVVRGKVPESGSDTHGAPGGNAAPRPSGAGQDQRIEQLR
                     RELREKDGENARLRSRLAAGSFGLPGGGVRPENMIWMFGSGRTGSSWLSTMMGDLEGH
                     ARWNEPYVGELFGSAYYIRAGDRMRGRKDYALGDDYREAWIHSIRNFVLEGANARFSG
                     LGENGYLAIKEPNGSIGAPLLAEAFPESRMILLVRDPRDVVSSAMAAQRKGSWGEGWR
                     ANGGGDSLADTDPDEFARQWANMYKVSLGNARKAYEAHKGPKAVVRYEDLRVDTLQTM
                     RRLYSTLNIPVGEQELARVVQRHAWENVPENKKGPDKPHRKATPGSWKEDLTPEQARI
                     VEELTVPILDEFYPDRSSSYGRVKYLEP"
     gene            complement(1539..>3461)
                     /locus_tag="VNT12_04735"
     CDS             complement(1539..>3461)
                     /locus_tag="VNT12_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWI45459.1"
                     /translation="DYHSVFARKNPLGLVEAFGQAFPEGSGASLVLKSINSEHYPGEH
                     GRLMEAAKGHRDIHVIDRYVTAAEKNAMFAGCDCYVSLHRSEGFGNTLAEAMYLGKPV
                     IATGYSGNMEFMTPQNSYPVDYTLRPIGDDAGPYPATSEWAEPDVGHAARLMRDVFEN
                     RGEASERGLRAAEDLRRNHSAEAAGRAMAERIRRVGTRLGAGPDAGRPHVHSQAGLDT
                     NPLTHRIATTPQPAASSSTLGRVLAPFRRVLLKLMWPFALQQRAVNEELLAKTKEADQ
                     SLRAVLNQLESRYAKDRADLLGAVNHNREQARTQGERLDRLEKRLAEGAGETPAPQTG
                     SVSKQSEDPGPTAAGTGSFSKQAISRLVTPFTAQQQKINDQLFTAVRTLGEKLGGLVS
                     RVEAADRLVAETRALPYVSGSPFEIFEEPVAGRVQGYGRRNGDPERAGTYDAFEDIFR
                     GPENFIRDRQRRYLHLLGDREPVLDIGCGRGEFLDLLRERGLEYAGIDPNPDMVRRCR
                     AKGHENVEVADANSYLEKCADDSIGAIFCAQVIEHMPYEELLRFYGLGLRTLEPGGLF
                     ILETVNPHSVPALKTFWVDPTHQHPLFPEVALALCEISGFESAYVFHPNGKGQVEVDR
                     YETGEYAVVAKRAAHE"
BASE COUNT          533 a         1162 c         1049 g          717 t
ORIGIN      
        1 gtcggcggcg aaaaaccgtc cctcgccctt gcagagtata tcggggtgac cgtccagcgt
       61 cctcatcagc caggaggtgc ctgacttgcc cacccccgta acgaagaaca cgggggtttt
      121 ggatcctcgt tctcgacgcg caggtttgcg tctcgccgaa gggatcctga ccgctcctct
      181 tgtcagcgca ggcaccttgc caaggcgcca tccgagtcct cttgctatcc ccttgaggcc
      241 tgtcacaccc gtctccaggt cgatgaacag actagtattt cgtccgagcc tgagcgtcat
      301 cttcttcttc agaataggca ttgctctcca ttcgaagtca caggcctggc gttatctggc
      361 tcttcacccg ctgcttgccc cattcacggc tcgagatact tcaccctccc gtaggaagag
      421 ctcctgtcag ggtagaactc gtcgagtata ggaaccgtca actcttctac tattctggcc
      481 tgctcggggg tgaggtcttc cttccagctt cccggggttg ccttacggtg gggtttgtcc
      541 ggtcccttct tgttctcggg cacattctcc cacgcgtgcc tctggactac cctggcaagc
      601 tcctgctcgc cgaccgggat attcagcgtc gagtagagtc gtctcatggt ctgtagggtg
      661 tcgacccgca ggtcctcgta gcggacgaca gccttgggcc ctttgtgggc ctcgtaggct
      721 tttctggcgt tgccgaggct caccttgtac atgttggccc attgccgcgc gaactcgtca
      781 gggtcggtgt cggcaagcga atcgccgccc ccgttagccc gccacccttc gccccagcta
      841 cccttcctct gggctgccat ggctgaggag acaacgtccc tggggtcgcg aaccagcagg
      901 atcatacgac tctccgggaa ggcttcggcc agcagcggcg ccccgatgga gccgttgggc
      961 tctttgatag ccaggtagcc gttctcacca agccccgaga agcgcgcgtt ggcgccctcc
     1021 agcacgaagt tgcggatgga gtggatccag gcctccctgt aatcgtctcc taaggcgtag
     1081 tctttgcggc cccgcatcct gtctccggcc ctgatgtagt aagccgagcc gaagagctcg
     1141 ccgacgtacg gttcgttcca cctggcgtgg ccttcgagat cccccatcat ggtgctcagc
     1201 cagctacttc ctgtccgtcc cgagccgaac atccagatca tgttctctgg ccttacgcct
     1261 ccgccaggca ggccgaaact ccctgcggcc agccgagatc gcagcctggc attctccccg
     1321 tccttctccc tgagctctcg acgcagttgc tcgatcctct ggtcctgtcc ggcgcctgaa
     1381 ggccgcggtg ccgcattccc tccgggagcc ccgtgcgtat ccgaccccga ttcggggacc
     1441 ttcccgcgca cgaccccagc ttccaggttc aagaaagccc tcacgtttcg gaccgctaat
     1501 gctacgcgct ggctgaagac cggcatccta cgattttctc attcgtgcgc cgccctcttt
     1561 gctacgaccg cgtactcgcc cgtctcgtac cggtcgacct caacctggcc cttcccgttg
     1621 gggtggaaca cgtaggcgct ctcgaagccg ctgatctcgc agagcgccag cgccacctcg
     1681 gggaagagcg gatgctggtg ggtggggtct acccaaaagg tcttcaacgc ggggacagag
     1741 tgcgggttta cggtctccag gataaagagt cctcctggct cgagcgtccg caagcccagg
     1801 ccgtagaagc gtaagagctc ctcgtagggc atgtgctcta tcacctgggc gcagaagatg
     1861 gcgcctattg agtcgtcagc acacttctcc agataggagt tcgcgtccgc gacctccacg
     1921 ttctcgtggc ccttggccct gcaccgccgg accatgtcgg ggttggggtc tatgccggcg
     1981 tactccaagc cccgctcgcg caggagatcc agaaactccc cgcgtccgca cccgatatca
     2041 agtacgggct cgcggtcacc gaggaggtgg aggtaacgtc gctggcggtc acggataaag
     2101 ttctctgggc cgcggaagat atcctcgaac gcgtcgtagg ttcccgcccg ctcaggatca
     2161 ccgttacggc ggccgtagcc ctggacacgc cccgccaccg gctcctcgaa gatctcgaac
     2221 ggggagcctg atacgtaggg tagagcgcgg gtctcggcca ccagccggtc tgcggcctct
     2281 acgcgcgaaa cgagcccgcc cagcttctca ccgagcgtac ggaccgcagt gaagagctgg
     2341 tcgttgatct tctgctgctg cgccgtgaac ggggtgacaa gacgggatat cgcctgcttg
     2401 gagaagctgc cagtaccggc cgctgtcggg ccgggatcct ctgattgctt gctgacacta
     2461 ccagtctgtg gggctggtgt ttctccggcg ccctccgcca accgcttctc caggcggtcg
     2521 agccgctcgc cctgcgtcct ggcctgctcc ctgttgtggt ttacggcgcc cagcaagtcg
     2581 gcgcggtctt tcgcgtaacg tgactcgagc tggttgagga cggcgcgcag gctctgatcc
     2641 gcctcctttg tcttggccag gagctcttcg ttgaccgccc gctgttgcag ggcgaagggc
     2701 cacatgagct tcagcagaac ccgccgaaag ggggcgagga ccctgcccag ggtggagctc
     2761 gaagccgctg gctgtggagt ggtcgcgatg cggtgcgtaa gcgggttggt atcgagccct
     2821 gcctggctgt gcacgtgtgg cctgcccgcg tccggtcctg cgccaagccg cgtcccgaca
     2881 cgtcggatgc gctccgccat cgcccttcct gcggcctcag cggagtggtt tcggcggagg
     2941 tcctccgccg ccctcaggcc gcgctcggat gcctcccccc ggttctcgaa gacgtcgcgc
     3001 atcaggcgcg cggcgtgccc gacgtcgggc tcggcccact cgctggtggc gggataaggc
     3061 ccggcgtcgt cgccaatggg ccttagcgtg tagtcgacgg ggtaactgtt ctgcggcgtc
     3121 atgaactcca tgttgcccga gtatccggtt gcgattaccg gcttgccgag gtacatggcc
     3181 tcggcaaggg tgttgccgaa gccttccgag cggtgaagcg agacgtagca gtcgcagccg
     3241 gcgaacatcg cgttcttctc ggccgccgtg acgtaacggt ctataacgtg gatgtccctg
     3301 tggcccttcg cggcttccat cagcctcccg tgctctcccg ggtagtgctc gctgttgatg
     3361 gacttgagca ccagtgaggc ccccgaaccc tcggggaaag cctgcccgaa cgcctccacc
     3421 aggccgagcg ggttcttccg cgcgaagacg ctgtggtagt c
//
LOCUS       DATMJA010000200         3789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_145542, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000200 DATMJA010000000
VERSION     DATMJA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145542"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1203
                     /locus_tag="VNT12_04740"
     CDS             250..1203
                     /locus_tag="VNT12_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI45460.1"
                     /translation="MDHNDFAERVERIVGNVELAVSGKRDSVFLAVVAMLAGGHVLIE
                     DVPGVGKTLLAKSLARSLRAEFRRIQFTPDLLPADVTGLNVYNQGEGDFEFWAGPVFA
                     NVVLADEINRASPKTQSALLEAMEEGSVTVDGVTRRLSQPFCVVATQNPVEHEGTYPL
                     PHAELDRFMIKMQVGYPDEDAETEMLKLSQDPVADLSPVVGVEEFLRMRQLVEEIYTS
                     EAVLRYVVSVTSATREHEDVYLGASPRASRMLLAASRARAAANGHSYCAPDDVKAMAS
                     SVLSHRIIPSATSSENGLGPDNATREIVQDILRSVPVTEAV"
     gene            1210..2457
                     /locus_tag="VNT12_04745"
     CDS             1210..2457
                     /locus_tag="VNT12_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWI45461.1"
                     /translation="MPGRGASRLSVRPTARGWQALFAGVVTLVVARLIGTTQFHQLAY
                     ALLALPLASLVLGVVGSRGLRYSRTLPPGVRITAGSPAKLRLLLERSRFGALKASVVD
                     RLPGPRSFDFSATRERSRIEVSLTFARRGVYTLGPAEVRVADPFGLLRFARRDWETTE
                     VVVYPQVYELRGFPLRGGNVEAGNTGSRGRRGEEFANLREYRRGDDRRHIHWKSLART
                     GELFVKEFSLQAPRRHTVALDLRREGLRTQENEIEDAVSAAASVLTHLAGESLPFRLL
                     DTGSSGAGTGFESDEGSYWEAMRHLATARADGTRHLGETVLGERGALGEGVVLVSRTR
                     DDELPYCVRKLREAGLSVIVVALATYTYRTPPVPGSAAQGREAEFLRSVGTLEAAGAT
                     VRVVTHPAGVAGLSDARGVEAVR"
     gene            2463..>3789
                     /locus_tag="VNT12_04750"
     CDS             2463..>3789
                     /locus_tag="VNT12_04750"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HWI45462.1"
                     /translation="MGTRLWLYGAALATGSAFCILFTGETYDGADGGFGALSVVTIIA
                     GHKAMLPLLLAAAVAALVGSAGRWRFVLLLPAVAAYTVVAVYGTDLFSVSGWQGFFAI
                     IWGDVYGAANTMYVQPIPYDLAPGLFVVLVPLVMILVAFATSATLYEESPVISVAVLG
                     LTIGVLSTISFEDGAGPFFAVFLVCAVGLLLSAGAAGPDGPGRPAVVAGAIVIALVLL
                     VPRMPFSDETVSTGWIDWTRIGTGGTSRLGVQADVGDYLTAGREAELLRIRSSEPLLW
                     RGGTMNHFDGVRWSDTTQPGADNGEEIAPEVETRPVLQRVQVLNAQTDLIFGGYKIVR
                     TSEFLGATPNSDGSWSSDEPFEEGDSYEVLSEIPQPTGAQLRGAGSDYPSTVEEKFLQ
                     LPEDTPPVVSETADKIQRRYEPATPYDRARAIEQYLTYDGGFTYNLDVSY"
BASE COUNT          633 a         1211 c         1253 g          692 t
ORIGIN      
        1 ccaagccact atgtcacact gttctacgga tcgccagacg gcgtgatttt gtcctttaag
       61 gcctccgctt gtcagcacgg cggctgcgcc gccttctcag cacgcttcgg gcctaaaggt
      121 cctcgctttc agcatgtcag ccagggtctg cacaaactaa ccggctgaaa tgctgacaag
      181 ctcccgaagg gagcgtactg acataagata taattccctc tgtcatccga gcacatagac
      241 aggactcctt tggaccacaa cgattttgcg gagagggtag aacgcattgt cgggaacgtc
      301 gagcttgctg tttctggcaa acgcgatagt gtcttcctgg ccgtggtagc catgcttgcg
      361 ggcggacacg tccttataga ggacgtaccc ggtgttggca agacactgct ggccaaatcg
      421 ctcgcccgtt cgctgagggc cgagtttcgc aggatacagt tcacccccga cctcctgccg
      481 gccgacgtga ccggcctcaa cgtctacaac cagggcgagg gtgacttcga gttctgggca
      541 ggacccgtct tcgcgaacgt agtgctcgcg gatgagatca accgcgccag ccccaagact
      601 cagagcgcgc ttttggaggc gatggaggag ggctcggtca cggtggacgg ggtgacgcgt
      661 cgcctctcgc agcccttctg cgtggtcgcc acccagaacc ccgtcgagca cgaaggcacc
      721 tacccgctgc cgcacgccga gctcgaccgg ttcatgatca agatgcaggt cggctacccc
      781 gatgaggacg cggagaccga gatgctcaag ctctcccagg accccgtcgc cgacctctct
      841 cccgtggtcg gggttgagga gttcctccgg atgcgacagt tggtcgagga gatctatacg
      901 agcgaggccg ttctcaggta cgtcgtctcc gtgacttcgg cgacccgcga gcacgaggat
      961 gtctatctag gcgcctcccc acgcgcaagc aggatgctac tcgccgcctc gagggctcgg
     1021 gcggcggcga acggacactc gtactgcgcg cccgacgacg tcaaggcgat ggcctccagc
     1081 gtgctctcac acaggatcat accctcggcc accagctctg agaacggcct cgggcccgac
     1141 aacgccaccc gtgagatcgt gcaggacatc ctgcgctccg tcccagtgac cgaggcggtg
     1201 taagggcact tgcctgggag gggcgcctcg cggctctccg tcagaccgac ggcccgcggc
     1261 tggcaggcgc tctttgccgg ggtcgtcacg ctggtcgtgg cgcgtctgat cggcactacc
     1321 cagttccacc agcttgctta cgcgctcctg gccctgccgc tcgcgtccct cgtcctcggg
     1381 gtggtgggtt ccagaggcct ccgttactcg cgcacactgc ctcctggtgt ccgtatcacg
     1441 gctggcagcc ccgcgaagtt gcgactcctg ctcgagcgct cgcggttcgg cgcgctgaag
     1501 gcgagcgtgg ttgaccgtct ccccggaccg cgcagcttcg atttttcggc gactagggag
     1561 aggagcagga tcgaggtctc cctaaccttc gcgcgcaggg gcgtctacac gctgggcccc
     1621 gcagaggtca gggtggcaga ccccttcggg ttactccggt tcgccaggag ggactgggag
     1681 acgaccgagg tggtcgtcta cccgcaggtc tacgaacttc ggggctttcc cttgcggggc
     1741 gggaacgtgg aggcaggcaa tacagggtcc cgcggccggc ggggcgagga gttcgcgaac
     1801 ctcagggagt accgcagggg cgacgacagg cgtcacatcc actggaagag tctggcacgt
     1861 acaggggagc ttttcgtcaa agagttctcc ctgcaggccc cgaggcgcca caccgtggcc
     1921 ctggatctgc gcagggaggg gctgaggacc caggagaacg agatcgagga cgccgtatcg
     1981 gcagcggcct cggttctcac gcaccttgcc ggggagagtc tgcctttcag gctgctcgat
     2041 acgggcagta gtggcgcagg cacgggtttt gaatccgacg aaggttccta ctgggaggcg
     2101 atgaggcacc tcgcgacggc gcgcgccgac gggacccgtc accttggaga gaccgtgctc
     2161 ggggagcgag gggccctagg ggagggcgta gtcctcgtct cacgcacccg cgacgacgag
     2221 ttgccgtatt gcgtccgcaa gctcagggag gctggtctct cggtgatcgt ggtcgccctc
     2281 gccacctaca cttacaggac gccccctgtg ccagggagcg ccgcccaggg ccgcgaggcg
     2341 gagtttctca ggagcgtagg cacgctggaa gcggccgggg ccacggttcg cgtcgtcaca
     2401 catccagccg gggtggcagg actttcggac gccagggggg tcgaggcggt gcgatgagtg
     2461 gattgggtac gagattgtgg ctctacggcg cggcgctcgc gactggcagc gccttctgca
     2521 tcctgttcac gggcgagact tacgacggtg ccgatggggg tttcggcgcg ctctccgtgg
     2581 tcacgattat agccgggcac aaggccatgc tgcccctgct cctcgccgcc gccgtggcgg
     2641 cgctggtcgg ctcggcgggc aggtggcggt tcgtgctcct cctccctgcc gtggccgcgt
     2701 atacggtggt cgcggtgtac ggtaccgatc tcttctccgt ctccggttgg caggggttct
     2761 tcgccattat atggggcgat gtctacggcg cggcgaacac catgtacgtc cagcccatac
     2821 catacgacct cgccccaggg ctattcgtcg tactcgtgcc cctggtcatg atcctggtgg
     2881 ctttcgcaac ctcggccacc ctctacgaag agagccccgt catctcggtg gccgttctgg
     2941 ggcttaccat aggcgttctc tcgaccatca gcttcgagga tggggctggg cccttcttcg
     3001 ccgttttcct ggtctgcgcc gtaggactct tactctccgc gggcgcggct ggacccgacg
     3061 gtcctggccg ccccgcggtc gtggccgggg ctatcgtgat cgcgctggtg ctcctcgtgc
     3121 cgaggatgcc cttctccgac gagacggtta gcacaggatg gatagattgg acgcgcatag
     3181 ggacgggcgg cacttccagg ctcggcgtac aggccgatgt gggagactac cttacggccg
     3241 gaagggaggc cgagctcctg cgcatccgaa gctccgagcc gctcctgtgg cgcggcggta
     3301 ccatgaatca cttcgacggt gtccggtggt cggacactac gcagccgggg gccgacaacg
     3361 gggaggagat agcccccgaa gtcgagaccc gtcccgtgct ccaaagggtg caggtcctca
     3421 acgcccagac ggacctcatt ttcgggggat acaagatagt gcgtacctcc gagtttctag
     3481 gggcgactcc gaactccgac ggctcgtggt cgagtgacga acccttcgaa gagggtgact
     3541 cctacgaggt gctctcggag atcccgcagc caaccggggc gcagctcagg ggcgcgggca
     3601 gcgattaccc gtcgactgtg gaggagaagt ttctccagct tcccgaagac acgccgcccg
     3661 tggtcagcga gacggcggat aagatccaac gccgatacga acccgcgacg ccatacgata
     3721 gggcgcgcgc gatcgagcag tacctgacct acgacggcgg ctttacctac aacctcgacg
     3781 tgagctacc
//
LOCUS       DATMJA010000201         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_618968, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000201 DATMJA010000000
VERSION     DATMJA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618968"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VNT12_04755"
     CDS             <1..1380
                     /locus_tag="VNT12_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45463.1"
                     /translation="DKPVEVSAEDDPELAERYAKVGQRGLPREDREMYESLGIEIKPF
                     DNLRIFAPLRYGMESGERIVHAEGIPARKLNTLRNARGEDARVYPIIWELGDVLPYAG
                     YVFEPSDMDDKFRGFIEELRDRGIRTTADFYALLDEIEDYFEAAREDGKNVSSWNGHN
                     HMTIAKVKNRFKVLPNKCGGLLAHGRVEHGGLPRADGPFEDNEMRVVDISQLTGVPQD
                     LIVTSVVSKIWELAETGRLGVDKLIIFVDELNKYAPSGNSRTSSLKDTLVDISARGRH
                     LNLTLFGAQQFRSKVDDEVIGNAATSLYGRIGDEELTNSSYRSFSGTTREELLQLEKG
                     QLLLRHAHYAVPVFGRFPRPPVLMGKQGTDIFGERRGDPAASVLAVMRNLTTRPPIIA
                     DIRSEIQGVPEEQVYEALDAVQAAHRTGHGAADPYKNFRWNLKRPARTGSGRRDASRI
                     LSGLDRMRD"
     gene            1625..2767
                     /locus_tag="VNT12_04760"
     CDS             1625..2767
                     /locus_tag="VNT12_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWI45464.1"
                     /translation="MSDTHLGYSRYAKLHPETSRNQREVDVQEAYGRAVDAILERDVD
                     LVVHSGDVFDSVRPATHVIIGFLKQTFRITREDIPYLVAAGNHETPRLRSTTAALEYA
                     NLVNAISAHGFEIDYEPVEVDGATVGVTLVPHGAVFGTGAVTPARDADVNILVTHGLV
                     PGLEARQHEMGEANLQPGMLEGGFDYIALGHYHDFHEHKPNAFYAGATERFGFGEVDS
                     RPGFAIVEFDGNGLKSVEHVEIETRPMLDLEKISARDMNATELTEAVLRRTSGIDLDG
                     TIVRLRAYDVRRGVASGMDRELLRDLQRRCLNFSLEVHAEERPEELERNGSSTAVFGP
                     LNEEFAAFVSERKERGELEAKFAEEFLEKGRRYLTRAASEEPESAA"
     gene            2764..>3090
                     /locus_tag="VNT12_04765"
     CDS             2764..>3090
                     /locus_tag="VNT12_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI45465.1"
                     /translation="MILERLFIENYKQLREPVELFPPEGAIGIVGANGAGKSTLFESI
                     LWAFFGSRGGGPRFANESIPWSGGSTKDPTTVEVTLATDGGSFTVRRRLKSGSTSAEA
                     LDAQGRT"
BASE COUNT          647 a          937 c          994 g          512 t
ORIGIN      
        1 gacaagccgg tcgaggtaag cgccgaggac gaccccgaac tcgcggagcg ttacgcgaag
       61 gtcggtcagc ggggactgcc gcgcgaagac agggagatgt acgaatcctt gggaatagag
      121 ataaagcctt tcgacaacct gaggatcttc gcccccttgc gttacgggat ggagtcaggg
      181 gagcggatcg tgcacgccga gggtatcccc gcccgcaagc tgaacacctt gaggaacgcg
      241 cgcggagaag atgctcgggt ctacccgatc atctgggagc taggggacgt gctgccctac
      301 gccggctacg tcttcgagcc atcagacatg gacgacaagt tcagggggtt tatagaggag
      361 ctgcgcgaca ggggcatcag gacgacggcg gacttctacg ctctgctcga tgagatcgaa
      421 gactacttcg aggccgcccg cgaggatggc aagaacgtct catcctggaa cggccacaac
      481 cacatgacca tcgccaaggt caagaaccgc ttcaaggtgc tccccaacaa gtgtggaggc
      541 ctcctggcgc acggccgcgt cgaacacggc ggcctgccgc gcgcggacgg cccgttcgag
      601 gacaatgaga tgcgcgtcgt ggacatctct cagctcaccg gggtgcccca ggacctcatc
      661 gtgacgagcg tcgtatcgaa gatctgggag ctcgcggaga caggccgtct cggggtggac
      721 aagctcataa tcttcgtcga tgagctaaac aagtacgccc cttccggcaa ctcccgtacg
      781 tcgtccttga aggataccct cgttgatatc tcagcccgtg gccggcacct caacctcacg
      841 ctcttcgggg cgcagcagtt caggagcaag gtggatgacg aggtgatcgg caacgccgcg
      901 acgagcctct acggccgcat cggcgacgag gaactgacca actccagtta ccgctctttc
      961 tcgggcacga ctcgcgagga gctgctccaa ctcgaaaagg gccagctcct tctcaggcac
     1021 gcgcactacg ccgtgcccgt cttcggccgg ttcccgcgcc cacccgtcct catgggcaag
     1081 caaggcacag atatcttcgg cgaacggcgc ggtgacccgg cggcttcggt cctcgccgtc
     1141 atgcgcaacc tcacgaccag acctccaatc atcgccgaca tccgatccga gatacagggc
     1201 gtgccggaag agcaggttta cgaggccctc gacgccgtgc aggcggccca ccgcacgggc
     1261 cacggcgcag cggacccata caagaacttc cgctggaacc tcaagcgccc ggcgaggacc
     1321 gggtcgggcc gccgcgacgc gtccaggatc ctctctggcc tggatcggat gagagactag
     1381 caaggacagg ctcgcgcgac tgcgatggat ttcgagatag tcggagagat atccgacgtc
     1441 gaaaccatag ccataggtag caggatcagt gacatcaccc atcaatccgt acaggaactt
     1501 gtgctggagc cacatgttcg gcggttagtc acgaaactac ctcgtgaact acccgatgat
     1561 ccggctagaa tagcgtgagt cttttagtag agaggatcat taagacatag tccgcatagc
     1621 ccacatctcg gatactcatc tagggtacag caggtatgcg aagctccatc ccgagacgag
     1681 ccgcaaccag cgggaggtgg acgtccagga ggcttacgga cgtgctgtcg acgccatcct
     1741 ggaacgggat gtggacctag tggttcactc cggcgacgtc ttcgattctg tgcgccctgc
     1801 gacgcatgtg atcatcggct tcctgaaaca gaccttccgc atcacacgag aggacatccc
     1861 ctacctggtc gcggcaggca accacgagac accgcgcctg cgttctacga ccgcggccct
     1921 ggagtacgcg aacctagtaa acgccatctc tgcccacggc ttcgagatag attacgaacc
     1981 cgtcgaagta gatggagcaa ccgtgggcgt aacgctagtc ccgcacggag cggtatttgg
     2041 tacgggtgcc gtgaccccgg cgcgggacgc ggacgtgaac atactcgtca cgcacgggtt
     2101 ggtgcctggc ctcgaggcgc gccagcacga gatgggcgag gcgaacctgc agccggggat
     2161 gctggaggga ggcttcgatt acattgccct cggccactac cacgacttcc acgagcacaa
     2221 accgaatgca ttctatgcag gggcgacgga gcgcttcggg ttcggggaag tagattcgag
     2281 gcctggattc gccatcgtgg agttcgatgg caatgggctc aagagcgtag agcacgtcga
     2341 gatagaaacg cggccgatgc tggacctgga gaagatctcc gcccgagaca tgaacgccac
     2401 ggagctgacg gaggcggtgc tacgtcgtac ctccgggatc gacctcgacg ggacgatagt
     2461 gcgcctcagg gcctatgacg tgaggcgcgg ggtggcgagc ggcatggacc gggaactgct
     2521 gagagatctg caacgccggt gcctgaactt ctccctggaa gtccacgccg aggaacggcc
     2581 cgaggagctg gagaggaacg gctcctcgac cgccgtcttc ggaccgctga acgaggagtt
     2641 cgcggcgttc gtctctgagc gcaaggagcg cggcgagctc gaggcgaagt tcgcagaaga
     2701 gttcctggag aaggggcgca ggtacctgac gcgggctgct agcgaagagc cggagagcgc
     2761 cgcgtgatcc tggagcgcct cttcatagag aactacaagc aattgcgaga gcccgtcgag
     2821 ctctttccgc cggagggggc gatcgggatc gttggcgcaa acggcgccgg caagtcgacc
     2881 ctcttcgaga gcatcctgtg ggcgtttttt ggctcccgcg gcggcggacc gcgcttcgcc
     2941 aacgagtcta tcccctggag cggcggctcg acgaaggacc ctaccaccgt cgaggttacc
     3001 ctggctactg acggtggctc gtttaccgtg cggcggcggc tcaagagcgg ctcgacgagc
     3061 gccgaggctt tggatgctca gggcagaacc
//
LOCUS       DATMJA010000202         4669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_619239, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000202 DATMJA010000000
VERSION     DATMJA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619239"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VNT12_04770"
     CDS             complement(<1..513)
                     /locus_tag="VNT12_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45466.1"
                     /translation="MIALQERYWGVPAKGVGSPGAALGPKRVSSREGVIRLERETKNQ
                     FFGPNLGYVLELYENYREDPDSVDGQMREFFESWSPPRVGTPGANGQQVAPEVDVEKA
                     VGASKHVRHIRDFGHLAAKLDPLGSEPPGDPTLDPTFYRITDEDLETLPSLIIGGPIA
                     ERTSNAREAAE"
     gene            665..982
                     /locus_tag="VNT12_04775"
     CDS             665..982
                     /locus_tag="VNT12_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45467.1"
                     /translation="MEDRIDYFERMLADNPDNPTGLLALANEYEKAGRDEDEAAVLER
                     YLAVHVDDEGNAYARLGSVLSNLGRKDEARAAYEKGIAQSEKHGHSGMADDLRLALIQ
                     LNE"
     gene            1250..1774
                     /locus_tag="VNT12_04780"
     CDS             1250..1774
                     /locus_tag="VNT12_04780"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI45468.1"
                     /translation="MDLGTRPTANEVKAMLGLEPHPTCGFVAETYRSPLRIPAGALPE
                     AYEGDRPYGSALYFLVAPDAQIVMHRIRSDQLYHHYMGDPLEVLMLYPDGTGAVTTVG
                     SDLAAGERPQLFVPGYTFHTSRLGPDADYALLASTEWPGVEPPDVEHGDIEALVESYP
                     DMAEHIRAFTSGAR"
     gene            1805..3415
                     /locus_tag="VNT12_04785"
     CDS             1805..3415
                     /locus_tag="VNT12_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI45469.1"
                     /translation="MSMGYDAVVIGAGHNGLTCACYLAKAGLKVLVLERCHDVGGMTL
                     TEELTLPGFRSDVHASGYQLANLSPVPDELKLEKHGLELNQPDLVYAHAFPDGRVLAV
                     SRSLEKTVENIGRYSKKDAETWRGLVERYRAAKDKIVASLFSAPPAFSAEAAALERSP
                     AGMDEYRFGLQSMRSWCDQTFESEEVKCLFGAFALFMAHAPDDAGGAELAWLFGTVLQ
                     DAGNNLVKGGMHHVSLALADHLRSLGGEIRTSAGVEKILVGSGRATAVRLESGEEIPV
                     GRLVASGVDPGQLALRFLGEGVVGPEIAGKMERYEWGDAACVIYAALDGPVEYKAGPE
                     AGAACHVHLTSPSLASVARASVECRGGELPATPLVVAWNDSVIDPSRAPEGKHLKKFV
                     VLGVPYEVKGDATGHVRERDWDDAKEGYADYLIDRITADYIPDLKEKLLKREAHSPLD
                     LERKLSSAVHGTICHGAMLPYQTGSMRPIPEMGDYRSPVENVYLCGSGSHPGPGVSMA
                     PGRNAAETIFPDLGLDFGTTVATGATRS"
     gene            3427..4638
                     /locus_tag="VNT12_04790"
     CDS             3427..4638
                     /locus_tag="VNT12_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HWI45470.1"
                     /translation="MKQPRRFETRAVHSGERRGGVDENGSFFPISTPIYASTTFSHHD
                     IETTDRVLGGEEPGYSYARYDNPTVVAFEEALASLEAPDGEARAFAFASGMSAMHAAL
                     TAAELGPGATVLAAEQLYGSTATLLLQIFGANGVEVRFVDAYDLGAVEKKVAELEPRA
                     VVIETISNPLLRVADTAGVAEVARAGGAALVVDNTFGTPFLQRPLELGADLVVHSATK
                     YLSGHGDLTAGVVAAGPPYDGALESIRKLVGGTLGPFEAWLAHRGLKTLPLRIARQCE
                     NARLISARLASHPKVAKVHHPALDDHPDHAVASRVLSDTGGLVSFELEAGGREAAYAF
                     LNALKLCVKAPSLGDIYTLAIHPATSSHRELSPTRRERLGVRENLIRLSCGIEHPQDV
                     ISDLERALEKS"
BASE COUNT          863 a         1531 c         1496 g          779 t
ORIGIN      
        1 ctcggcggcc tccctggcgt tggacgtacg ctcggcgatg ggtccgccta tgattagcga
       61 gggcagggtc tccaggtcct cgtcagttat cctgtagaag gtcgggtcga gcgtcgggtc
      121 ccctggcggc tcgctcccga gagggtcgag cttcgcggcc agatgcccga agtctcttat
      181 gtgccttacg tgcttggagg cccctacggc cttctccacg tcgacctcgg gagcgacctg
      241 ctgcccgttg gcgccaggcg tccccacccg cggcggactc caggactcga agaactcccg
      301 catctgtccg tcgacggaat cggggtcctc cctgtagttc tcgtagagtt ccaacacgta
      361 gccgaggttc ggcccgaaaa actggttctt cgtctctctc tccaaccgga tcacaccctc
      421 acgtgacgat accctctttg gcccgagcgc cgcaccagga gacccaacac ccttcgcggg
      481 caccccccaa taacgctcct gcaaagcgat caaacatctc gatctttcct atgatacccc
      541 acatccccag taccgtaccc acccctatac ctacctgaat agctggtcca ttctacccgc
      601 tcgacgctag ccaggcgcac cttgacctat actggcgcag acgaaacgtg aagggaggaa
      661 cctcatggaa gacaggatag attatttcga gcggatgctg gccgacaacc ccgacaatcc
      721 gacggggttg ctggcactgg ccaacgagta cgagaaggcc ggacgcgacg aagacgaggc
      781 ggcggttctg gagcgctacc tggccgtcca cgtcgacgat gagggaaacg cttacgcgcg
      841 tctcggcagc gtgctctcca acctcgggcg taaagatgag gcgcgtgccg cctacgagaa
      901 ggggatcgcc cagtccgaga agcacggcca cagcgggatg gcagacgacc tcaggctcgc
      961 acttatacag ctaaacgagt aggtcctgtt ccagataatg cgaccctgcg agagcaattt
     1021 ctgcgccgta acaaaatcga tacacgggcg ttaccacccc ttaacgtatc tggcccgata
     1081 atgcgagcag gctccccggc gaagtagagg atccccctcc gcttgcacaa cacgtcgggg
     1141 agcgatcttt gggatggcca tacgcccgtc ccgagtagac ttccggaaga tacacggata
     1201 tgctcctggc gtgtcatgac cggagataga gagccccagg gaggagacga tggacctagg
     1261 aacacgtccg acggcgaatg aggtcaaggc tatgctcggg ctcgagcccc accccacatg
     1321 cggcttcgtc gccgagacct accgcagccc actgaggatc cctgccgggg cgttgcccga
     1381 agcctacgaa ggcgaccgtc cctatggctc ggccctctac tttctcgtcg ctcccgatgc
     1441 ccagatcgtc atgcacagga tacgctccga ccaactctac caccattata tgggagatcc
     1501 gctagaggta ttgatgctct atcctgacgg cacaggcgcc gtgacgactg tgggttcgga
     1561 cctggcggcg ggcgaacgtc cgcaactgtt cgtccctggt tacaccttcc acacttcacg
     1621 cctggggccg gacgccgact acgcccttct ggccagcacc gagtggcccg gggtggagcc
     1681 ccctgatgtg gagcacgggg acatcgaggc gctggtcgag tcctaccccg atatggctga
     1741 gcacatccgc gctttcacga gcggagcacg atgaccccac atggcacgtt cgggaggtag
     1801 agtgatgtcg atggggtacg atgctgttgt tatcggcgcc gggcacaacg ggttgacctg
     1861 cgcctgttac ctcgcgaagg cgggactgaa ggtgctcgtc ctcgagcgct gccacgacgt
     1921 agggggcatg acgctcaccg aggagttgac gctccccggg ttcaggtcgg acgtccacgc
     1981 gagtggctac cagctggcca acctctcccc tgttcccgat gagctaaagc tggagaagca
     2041 cggccttgag ctgaaccagc ctgatctggt ctacgcccac gccttccctg acggcagggt
     2101 tctggccgtg agcaggagcc ttgagaagac cgtagagaac atcggccggt actcgaaaaa
     2161 ggacgccgag acctggcgcg ggctggtcga gcgctacagg gcggcaaagg ataagatcgt
     2221 cgcctcgctc ttctcggcgc cgccggcgtt ctcagccgag gcggccgcgc tggagcggag
     2281 ccccgccggg atggacgagt accgcttcgg cctgcagagc atgcgctcgt ggtgcgacca
     2341 gaccttcgag tccgaggagg tcaagtgcct gttcggcgcc ttcgccctgt tcatggcaca
     2401 cgcccccgac gacgcaggcg gcgccgagct cgcctggctt ttcggcaccg tgctgcagga
     2461 cgcggggaac aacctggtga aaggcggcat gcaccacgtg tcgctggccc tcgccgacca
     2521 cctccgttcc ctggggggtg agatcaggac cagcgcaggt gtggagaaga tcctcgttgg
     2581 ctcgggcagg gccacggcag tccggctgga gagtggcgag gagatacccg tcgggcggct
     2641 cgtggcctcg ggggtagatc ccggacaact cgcgctcagg ttcctgggcg agggggtcgt
     2701 cggccctgag attgcaggca agatggaacg gtatgaatgg ggagatgcgg cgtgcgtgat
     2761 ctacgcggcc ctggacggcc cggtcgagta caaggcagga cccgaggccg gcgccgcctg
     2821 tcacgtgcac ctgacttccc cgtcgctggc ttccgtggcc agggcttcgg tcgagtgccg
     2881 cgggggagag ctaccggcca cacccctcgt ggtagcctgg aacgactcgg tgatagaccc
     2941 gagcagggct cccgagggca agcacttgaa gaagttcgtc gtgttaggcg tgccctacga
     3001 ggtcaagggc gacgcaaccg ggcatgtcag ggagagggat tgggacgatg cgaaggaggg
     3061 gtacgccgac tacctgatcg acaggatcac cgctgactac ataccagacc tgaaagagaa
     3121 gctgctgaag agggaggcac attcgccgct cgacctggag aggaagctga gcagcgccgt
     3181 gcatgggacc atctgccacg gcgcgatgct accttaccag acgggctcga tgcgtcccat
     3241 acccgagatg ggagactacc gctcccctgt agagaacgtc tacctgtgcg gctcggggag
     3301 ccaccccgga ccaggcgtct cgatggcccc cgggaggaac gcggccgaga cgatcttccc
     3361 cgatctcggc ctcgacttcg ggacgacggt cgccaccggg gcaaccaggt cgtagtataa
     3421 gccaccatga agcagccgag acgtttcgag acccgcgccg tccactccgg cgagcgcagg
     3481 gggggtgtgg atgagaacgg gagcttcttc cctatctcga ccccgatcta cgcctcgaca
     3541 accttctccc accacgacat agagactacg gacagggtcc ttggcggcga ggagccgggc
     3601 tacagctacg cccgctacga caacccgacg gtggtcgcct tcgaggaggc gctggcctcg
     3661 ctcgaggctc ctgacggcga agccagggcg ttcgcgttcg cctcggggat gtccgcgatg
     3721 cacgccgctc tgacggcggc ggagcttggc ccgggggcca cggtcctggc ggccgagcag
     3781 ctctacggct ctacggccac gctcctgttg cagatcttcg gcgcgaacgg ggtcgaggtc
     3841 cgattcgtgg acgcctacga tctaggggcg gtggagaaga aggtagcgga gctcgagccc
     3901 cgcgccgtcg tcatcgagac catctccaac cccctcctgc gcgtggccga caccgcgggc
     3961 gttgccgagg tcgcgcgcgc gggcggcgca gcgctcgtgg tggacaacac cttcggcaca
     4021 ccctttctgc agcgtccgct cgagctgggc gcggacctgg tagtccacag cgcaaccaag
     4081 tacctctcgg gccacggcga cctgaccgcc ggggtcgtcg ccgcgggtcc tccgtatgat
     4141 ggagcgctgg agagcatcag gaagctcgtc ggcggcactc tgggaccctt cgaggcctgg
     4201 ctcgcccaca ggggcttgaa aacgttgccg ctgcggatcg cccgccagtg cgagaacgcg
     4261 cgcctgatct ccgcccgcct cgcctcccac cccaaggtcg cgaaggtcca ccatccggcc
     4321 ctggatgatc acccagacca cgcggtcgcc agccgcgttc tctccgacac aggagggctc
     4381 gtctccttcg agctggaggc cggagggagg gaggctgcct acgcgttcct gaacgccctc
     4441 aagctgtgcg tcaaggctcc gagcctcggg gacatctata ctctggcgat ccaccccgcc
     4501 acctcttccc acagggagct ctcgcccacg cgacgcgagc ggctcggggt gagggagaac
     4561 ctgatccggc tctcctgcgg catcgagcac ccacaggacg tgatctccga cctcgaaagg
     4621 gcgctcgaaa aaagctagtc agcacgcctg cttcgcaggc ttgtcagca
//
LOCUS       DATMJA010000203         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_145999, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000203 DATMJA010000000
VERSION     DATMJA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145999"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1397
                     /locus_tag="VNT12_04795"
     CDS             330..1397
                     /locus_tag="VNT12_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI45471.1"
                     /translation="MKATEGVFRLSGTGRSKSFVVAGLIMALSLVLAGCGGGASGQQN
                     GGGAVSGNIKIEGSSTVYPITQAAAELFRDENADARIEVGGAGTSDGFEAFCKGDTQI
                     SDASRPIDAAEEVPVCEQNGVEFIEIPIAYDGISVVVNKKGNDWAKDITSEELKTIWE
                     PSAEDKITRWSQVRSSWPDRPLDLYGPGTESGTYEFFNEVIVGNEEEVSRQSDVEMSE
                     DDNVLVQGVSGDANALGYFGYSYYENNLDTLRALAVDDVKPTEDSIRSGEYLLSRPLF
                     IYVSTDALKKNKAVEPFVDFYVSNQNLDRLVKAAKYVTLPASLEQASRAQYEDRTTGT
                     VFNKDGEPKGGDLETALEQSQ"
     gene            1493..>2166
                     /gene="pstC"
                     /locus_tag="VNT12_04800"
     CDS             1493..>2166
                     /gene="pstC"
                     /locus_tag="VNT12_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWI45472.1"
                     /translation="MVIRAEGARSGADAWKRNSRTREARERFIKVFLTACAYLSIFTT
                     FGIVIVLFEETSRFFLEVSPVEFLTSTRWAPPQGDFGVLPLVYGTLMATIIAISVALP
                     IGLLTAIYLSEYAPMRLRRWFKPALEILAGVPTVVYGYFALTFITPLFRDSLFSGIES
                     FNAISAGLIMGVMIIPTVGSLSEDAIYAVPLSLREGAYALGATRRETAMKVVVPAALS
                     GIVAAV"
BASE COUNT          422 a          637 c          693 g          414 t
ORIGIN      
        1 tacgacgtca ggttgccagg aagagcacgc gatctgcaac gcttcctgcc catcagcggc
       61 agtcatgagg tcgaatccct cgctctctag ccaacccgcg agagagaagc cctcggcaac
      121 gctttccgcc gcaaggagca acctgtgcga ctcttcagtc gagtgctttt ctgccatatc
      181 gaccaaagtt tgcatgcccg gcgcccgaat cctcaaggct tttgggcgca tgaaacgaat
      241 atttaatgcg tttcttaccc gcgtgtaaca caaggcataa aaccttgccc tactatcgaa
      301 gcgggtcaaa gttggccctc aagacatgtg tgaaagcgac ggagggagtg ttcaggttga
      361 gtggaaccgg gcgttcgaaa tcgtttgtag tggccggcct cattatggcg ctatcgctcg
      421 tgctagcggg ttgcgggggc ggtgccagcg gacaacagaa tggtggtggg gccgtctcgg
      481 gcaacatcaa gatcgaaggt tcgagcaccg tctacccgat cacccaggct gcggcggagc
      541 ttttccggga tgagaacgcc gacgcgcgca tcgaggtcgg cggcgcggga accagcgacg
      601 ggttcgaggc cttctgcaag ggtgacaccc agatctccga cgcttccagg ccgatagacg
      661 cggccgaaga ggtgcccgtg tgcgagcaga acggggtcga gttcatcgag atcccgatcg
      721 cttacgacgg catttcggtc gtcgtgaaca agaagggtaa cgattgggcc aaggacatca
      781 caagcgagga gctcaagacc atttgggagc cgtccgcgga ggacaagatc acgcggtgga
      841 gccaggtacg gtcgagttgg cccgataggc cgttggacct ctacggcccc ggcaccgagt
      901 cgggcaccta cgagttcttc aacgaggtca tcgtgggtaa cgaggaggag gtcagccgcc
      961 agtccgacgt cgagatgagc gaagacgaca acgtgctcgt gcagggtgtc tcgggcgacg
     1021 cgaacgcact gggctacttc ggctactcgt actacgagaa caatctggac acgctcaggg
     1081 cgctcgccgt ggacgacgtc aagcccaccg aggattccat ccgcagcggt gagtatctgc
     1141 tctcccgccc gctgttcata tacgtgagca ccgacgcgtt gaagaagaac aaggccgtcg
     1201 agcccttcgt ggatttctac gtctcgaacc agaacctcga ccggctggta aaggccgcga
     1261 agtacgtgac gctgccagcc agcctggagc aggcgtcgcg ggcgcagtac gaggaccgca
     1321 ccaccggcac cgtgttcaac aaggacggcg agccgaaggg cggcgacctt gagaccgcgc
     1381 tcgagcagtc gcagtaaccg tttgtaccgg gaaggatctg cttgtagcag aggggaaccg
     1441 gcttcgaagc cggtctccct ctttctgacc acgaagattc ggagggtttt ctttggtcat
     1501 tagagcggaa ggggcgaggt ccggggctga cgcctggaag cgcaacagcc gtacccggga
     1561 ggccagagag cgcttcataa aggtctttct caccgcctgc gcgtacctgt cgatcttcac
     1621 tacgttcggg atcgttatcg tgctcttcga agagacttcg cggtttttcc tggaggtgtc
     1681 cccggtggag tttctgacct ccacgcggtg ggcgccgccg cagggcgatt tcggtgttct
     1741 gccgctcgtc tacggtacgc tgatggcgac catcatcgcc ataagcgtgg cgcttcccat
     1801 aggactgctc acggccatct acctcagcga gtacgcgccg atgcgcttgc ggcgatggtt
     1861 caagccggcg ctggagatcc tggctggcgt gcccaccgtc gtgtacgggt acttcgccct
     1921 gacgttcatc accccgctct tcagggacag cctcttctcc ggcatagagt ccttcaacgc
     1981 gatctccgcc gggctcatta tgggggtgat gatcatcccg acggtgggct cgctcagtga
     2041 ggacgccatc tacgcggtgc cgctctccct gcgggaaggc gcctacgcgc tcggtgctac
     2101 caggcgagag acggcgatga aggtcgtcgt gcctgcggcg ctctcgggga tcgtggcggc
     2161 cgtgat
//
LOCUS       DATMJA010000204         6963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_441810, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000204 DATMJA010000000
VERSION     DATMJA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441810"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VNT12_04805"
     CDS             complement(<1..606)
                     /locus_tag="VNT12_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HWI45473.1"
                     /translation="MKESLTRRQFIWLAGVAGMAMVGIAYFLWRRRARVSGSITEVAR
                     HVPPGPYRIGAFIVALKIGRDPSDFVLSVAHSSRPERILWQSIPGESFVSAAEGKETV
                     HESSAHLTLEDEIRKLHPDQTIDRVEKRGTDLVITGRLTNGPDQGGVGYTISFSPVTD
                     GRLRFEAEVEQPCNRVYLTHASSPEERFFGFGTQFTYFDMKG"
     gene            complement(652..1464)
                     /gene="nudC"
                     /locus_tag="VNT12_04810"
     CDS             complement(652..1464)
                     /gene="nudC"
                     /locus_tag="VNT12_04810"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HWI45474.1"
                     /translation="MADLEQALWFAFQGERLLVFEDGPVRVPLADSPEELDIRFRWEI
                     GDLDGHACWAVEVDPDAGPPAGMVFRDLRSFFFGVDEDFFGMAGRAKQIVGWHETHRF
                     CVRCGGETERVPGELAMRCTRCGMMHFPRLSPAAIVLVKRGDRILLAHSPGFPQGLYS
                     VLAGFVEPGESIEEAVVREVREEVGIEVTNVRYFGSQPWPFPNSLMIGFTADYTGGDL
                     APEPGEIEDAGWYTADDLPKLPPKISIARAMIDDFVSQHGVDSEDQETSGSA"
     gene            complement(1457..4324)
                     /locus_tag="VNT12_04815"
     CDS             complement(1457..4324)
                     /locus_tag="VNT12_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWI45475.1"
                     /translation="MPDRPEPSVKQLHAALDGKLEGYLRTDEASRAMWSTDASIYLRK
                     PVGVVVARSEEDIELTLSTARDLGLSITPRGTGTSLAGQATAPGLALDVSEMRRALEI
                     DLAGRRCVVEPGLVQGELNAMVEPHGLVFGADTSTSDVATLGGMVGNNSAGMRSLVYG
                     MTSDQVLGLRCVLASGETVELAPLGREEARGRARHADALARLLRGALEIGDRYGDEIR
                     RRFPKMIRRVSGYGLDALIDPETLDLTRLICGSEGTLAVVARAEFRLHPLPEARALAS
                     FEFDTLSAAARATGRFLQMNPSAVELLDDVAIGRARANPAYRDSTQFVKGNPKAVLVV
                     EWSGAEQELDDRFTKLDELAGEAGALAATPLRTKAEMGQTVRLRKSTLPLLLGTTDKE
                     KPVAFVEDAAVPPDRLEEFVVRFEEIVEKNGTWACFYGHASVGCLHVRPALDTSDPDG
                     VSRMRRIAEEVANLVVDCGGSISGEHGDGLSRSEFMEKMYGGEILRAFADVKRLFDPR
                     GMMNPGVIVDPSPMDRQLRIGPGRRRLPIKTNLSFDDQGGFAKAVELCNGSGFCRKRT
                     GGTMCPSYMVTLDEQDTTRARANMLRSVIEGTLPPEELTGQRMKEVMGLCVGCKACKT
                     ECPSRVDVASMKTEVLYQTGREHGFSLRQKAAGHIRRQLALAALVPSLYNAIAGTSLA
                     RHAAALAGIDPRRSLPRVTKKSFSRRFPGLPQGTGPAEVAIFNDTWNEYQRPEVGEGA
                     VRLFAAAGARIHLPEVVCCGRPMLSEGLVDEAREHARRNLDLLMPLVERGVPLVGLEP
                     SCILTLRDDYRKLLPEDERVESLARATRLFEEAMLDLDRELPLREGAPLLLHGHCHQK
                     ALVGTAPTERLLGFAADVEVVDSGCCGMAGLFGYEKGHYEVSMKMGERRLFPAVREAE
                     KRVVVAPGTSCREQILAGTQRHALHPAEYLALLLDDTKTVG"
     gene            complement(4463..5023)
                     /gene="folE"
                     /locus_tag="VNT12_04820"
     CDS             complement(4463..5023)
                     /gene="folE"
                     /locus_tag="VNT12_04820"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWI45476.1"
                     /translation="MNRPRIESAVREILLAIGEDPGREGLRGTPQRVAETYALLFAGL
                     ADDPARHLDVGFQEEHREMVLIRDLPLYSLCEHHLMPFVGKAHVGYIPDRKVVGLSKL
                     ARLVEGYARRPQLQERLTAQVADAIQERLGARGAIFVIEAEHLCMTMRGVQKPGSVTV
                     TSAVRGIYARDPRTRQEAMSLITAHR"
     gene            complement(5031..5396)
                     /locus_tag="VNT12_04825"
     CDS             complement(5031..5396)
                     /locus_tag="VNT12_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45477.1"
                     /translation="MFSTDEMRAESHGGDEKMQLPTRASYVVMGAGIHGLSSAWHLAK
                     ESKAKGCGSGEEVIDDAYAIADSNHGYNMIGVGALVAKELMDEPQDLLEPFRFSRHET
                     ENCTPLATHPSHGTDARKL"
     gene            complement(5423..>6963)
                     /locus_tag="VNT12_04830"
     CDS             complement(5423..>6963)
                     /locus_tag="VNT12_04830"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWI45478.1"
                     /translation="PKAKYVVVSAITPTPLGEGKTTTTVGLGQAFKHIGKTATVAVRQ
                     ASMGPVFGIKGGAAGGGYSQVVPMDALNLHLTGDIHAVTEAHNQLAAMIDNHLYQGNA
                     LDLELQSITWRRVMDVNDRALRNIVVGLGAHTDGVPRQTGFDITAASEAMAILSLSTS
                     LHDMRSRFGRIVIGSDADGNPVTAEDLMGAGVMTVIMKEAIKPNLMQTLENTPALVHA
                     GPFGNIATGNSSIVADLIGIRGGDFLITEAGFGADMGAERFFNIKCRYSGLEPDAAVL
                     VATVRALKVHSGRYELRAGKPLPEEMQKENPDDVYAGAANLKKQIENIRLHGVSPVVA
                     INAFPTDHETEYDAIREIAGEMGARVAVCSHFLDGGAGAVELAEAVAEAAEEPTEFAF
                     LYPDEAPLREKIETIAKEVYGARGVEYDLIASRQLDSYQKIGFGELPICMAKTHLSIS
                     SDPSLKGAPTGWTLPVREVRASVGAGFVYPICGDMRTMPGLSASPAAEGIDVDENGEI
                     VGLF"
BASE COUNT         1201 a         2258 c         2147 g         1357 t
ORIGIN      
        1 tcccttcatg tcgaagtacg taaattgagt cccgaagccg aagaagcgct cctcagggga
       61 cgaagcatgg gtaaggtaga cccggttgca gggctgctcg acctcggcct cgaaacgcaa
      121 ccttccgtcc gtaacgggcg agaacgagat cgtgtagcct actcctccct gatcgggacc
      181 gttggttagc cgtccggtga tcaccaggtc ggtcccacgc ttctcgacgc ggtctatagt
      241 ctggtcggga tgcagctttc gtatctcgtc ttcgagggtg aggtgtgcac ttgactcgtg
      301 gaccgtctcc tttccctcag cggcggagac gaaactctct ccgggaatac tctgccacag
      361 tattctctcc ggcctggagc tgtgggcaac gctgagaacg aagtcagaag gatctcgtcc
      421 gatcttcagc gcaacgataa acgcgccgat ccggtacggt cccggaggga cgtggcgcgc
      481 cacttcggta atagaaccgg agacccgagc ccgccttcgc cagaggaaat aggctatgcc
      541 taccatggcc atccccgcca caccggcgag ccatatgaac tgcctccgcg tcaacgattc
      601 tttcaccgca atctcttccg gttctagttg ctcccccatc atacaagagg gttaggcact
      661 gccggatgtc tcctggtctt ccgagtcgac gccatgttga cttacgaagt cgtctatcat
      721 ggcgcgggcg atgcttatct tgggcggcag ttttgggagg tcgtctgcgg tgtaccagcc
      781 ggcgtcttcg atctcaccgg gttccggagc caggtcgcca cccgtgtaat ctgcggtaaa
      841 gccgatcatg agcgagttcg ggaaaggcca cggctggctc ccgaagtacc tgacgttcgt
      901 gacctcgatg ccgacctcct cgcgcacctc gcggacgacg gcctcttcta tagactcgcc
      961 tggctccacg aagcccgcga gcacactgta gagtccctgc ggaaatcctg gcgagtgtgc
     1021 cagcaatatc ctatcccctc tcttgacgag gacgatggcc gcagggctga ggcgcggaaa
     1081 gtgcatcatc ccacaccgtg tacacctcat cgcaagctct ccaggcaccc gctctgtctc
     1141 gccaccgcag cgcacgcaga accggtgcgt ctcatgccag ccgacgatct gcttcgccct
     1201 gcccgccatc ccgaagaagt cctcgtcaac cccgaaaaag aagctcctta ggtccctgaa
     1261 caccatgcct gcgggcggcc cagcgtcagg gtctacttcg acagcccaac aagcatgccc
     1321 gtcgaggtcc ccgatctccc acctgaacct gatgtcgagc tcctctggac tgtcggctag
     1381 cggcacacgg acaggcccgt cttcgaacac gagcagtctt tcgccctgga acgcgaacca
     1441 cagcgcctgt tccaggtcag ccaacggtct tcgtgtcgtc aagcagcagg gcaagatact
     1501 cggcagggtg cagggcgtgc cgttgtgttc cggctaggat ctgctcgcgg cagctcgtcc
     1561 ccggcgcgac gacgacccgc ttctccgcct cccgcacggc agggaagagc cttcgctcac
     1621 ccatcttcat cgagacctcg tagtgtccct tctcataacc gaagagtccg gccataccac
     1681 agcatcccga gtcgaccacc tcgacgtcgg cagcgaaccc gagcagccgc tccgtcggag
     1741 ccgtacctac tagcgccttc tggtggcagt gtccgtgcag gaggagaggt gcaccttccc
     1801 ttagcggaag ctctctgtcg agatcgagca tcgcctcctc gaagaggcgg gttgctctgg
     1861 ctaagctttc gacgcgctcg tcttcgggaa gaagcttcct gtaatcatcg cgcagggtca
     1921 ggatgcagct cggttcgagg ccgaccagcg gaacgccgcg ctctacgagg ggcatcagga
     1981 gatccaggtt acgcctggcg tgctctctag cctcgtccac caggccctcg gagagcatag
     2041 gcctcccgca acagacgacc tcgggcaggt gtattctcgc tcccgcggcc gcgaagagcc
     2101 ttacggcccc ctccccgacc tcgggcctct ggtactcatt ccaggtgtcg ttgaagatgg
     2161 ctacctccgc aggtccggtg ccctgcggca gcccagggaa gcgcctggag aaggacttct
     2221 tcgttacgcg cggcaacgag cggcgggggt ctatgcccgc gagggcggcg gcatggcggg
     2281 cgaggctcgt tcctgcgatt gcgttgtaaa gcgaggggac cagggccgcg agcgcgagct
     2341 gcctgcggat gtgtcctgct gccttctgac ggagagagaa gccgtgttct ctgcccgtct
     2401 ggtagaggac ttcggtcttc atgcttgcga cgtccacacg ggaggggcat tccgtcttgc
     2461 acgccttgca tccgacacag aggcccatga cctccttcat acgttggccg gtcagctcct
     2521 cgggtgggag ggtgccttcg atcacggacc tgagcatgtt ggcccgcgcc ctcgtggtgt
     2581 cctgctcgtc gagggtaacc atgtatgagg ggcacatcgt gcctcccgtt ctcttgcgac
     2641 agaaaccgct gccgttgcac agctcgacgg ccttggcgaa gccgccctgg tcatcgaagg
     2701 agaggttcgt ctttatgggg aggcgtctcc ggcctgggcc gatgcgtagc tgccggtcca
     2761 tcggcgaagg gtctacgata acgccagggt tcatcattcc tctggggtcg aagagacgct
     2821 tgacgtctgc gaaggcgcgc aggatctccc ctccgtacat cttctccata aactccgaac
     2881 gcgagaggcc gtcgccatgc tcgccggaga tcgagccccc gcaatccacg acgaggtttg
     2941 cgacctcctc ggcgatgcgc cgcatccgtg agacgccatc ggggtccgag gtgtcaagtg
     3001 caggcctgac gtgcaggcat cccacgctgg cgtggccgta gaagcaggcc caggtaccgt
     3061 tcttctcgac tatctcctcg aagcgaacga cgaactcctc cagccggtcc ggcggcacgg
     3121 ccgcgtcctc cacgaaggcg acaggtttct ctttgtctgt ggtaccgagc aggagcggca
     3181 gggtggactt gcggagtctg acggtctggc ccatctccgc ttttgtgcgc aacggcgtcg
     3241 cggccagcgc cccggcctca cctgccagct cgtcgagttt cgtgaagcga tcgtctagct
     3301 cttgttccgc gccgctccac tcaacgacca ggacggcctt cgggttgcct ttgacaaact
     3361 gggtcgagtc cctgtaggca gggttcgccc tcgcgcggcc tatcgcgacg tcgtcgagca
     3421 actctacggc cgacgggttc atctgtagga accggcccgt ggcgcgggct gcggcggaga
     3481 gggtgtcgaa ctcgaaagag gccagagcgc gggcctcggg gagcgggtgc agcctaaact
     3541 ccgcgcgggc aactacggcc agcgtgccct cggagccaca aatcaagcgc gtgaggtcca
     3601 gggtctcggg atctatgagg gcgtcgaggc cgtagcctga aacacgacgg atcatcttcg
     3661 ggaaccggcg ccttatctcg tccccgtagc gatccccgat ctccagggcc ccgcgcagca
     3721 acctcgcgag cgcgtcggcg tgacgggcgc gccctctggc ctcttccctg ccgaggggag
     3781 caagctcgac cgtctccccg gaggcgagca cgcagcgcag gccgaggacc tgatccgagg
     3841 tcatcccgta gacgagcgag cgcatccccg cggagttgtt cccgaccatg ccacctagcg
     3901 tcgccacgtc ggaggtagag gtgtccgccc cgaacacgag cccgtgcggc tcgaccatcg
     3961 cgttgagctc gccctgcacc agtccaggct cgaccacgca acgccgtcct gcgaggtcta
     4021 tctccagcgc ccggcgcatc tcggagacat cgagggcgag accaggggct gttgcctgcc
     4081 cggccaggga ggtgccggta ccgcgaggcg taattgagag cccgaggtcg cgagccgtcg
     4141 agagtgtgag ctctatgtcc tcttcagagc gggcgacgac gacgcccacg ggcttgcgta
     4201 agtagataga ggcatccgtg gaccacatgg cgcgtgaggc ttcatcagtg cgcaggtacc
     4261 cttcgagctt gccgtcgagg gcggcgtgga gttgcttgac ggaaggttcg ggtctatccg
     4321 gcatggtcct gtcaacttta catgcagaag ggacgtgtcg ccaatcaggg atccccgcct
     4381 tcttcggcga gactgttgta ggcctgggag cgctgcccct cctcgcaaaa ctccacgacc
     4441 acggccatct ctcccgtggc acctagcgat gggcggttat gaggctcatg gcctcctggc
     4501 gggtgcgcgg gtctctggcg tagatgccgc gcacggcgct tgtgacggta acgctccctg
     4561 gcttctgcac gcccctcatc gtcatgcaca gatgttcggc ctcgatgacg aagatggcgc
     4621 cacgggctcc caacctctcc tggatagcgt cggcgacctg ggcggtaagg cgctcctgga
     4681 gctgggggcg acgggcgtaa ccctccacca accgggccag cttggacagg cctacgacct
     4741 tcctgtctgg tatgtagcct acgtgggcct tgccgacgaa aggcatgagg tgatgttcgc
     4801 agagcgaata caggggtagg tccctgatca ggaccatctc gcgatgttct tcctggaacc
     4861 ctacgtcaag atgcctggct gggtcatcgg caagccccgc gaagagcaac gcgtaggttt
     4921 cggcgacgcg ctgcggcgtg cctctgaggc cttcccgtcc cgggtcctcg cctatggcga
     4981 gaagtatctc cctgacggcg ctttcgatcc gaggcctgtt cacccggtgt tcagagcttc
     5041 cgtgcgtcag ttccatggga agggtgagtg gctagtgggg tgcagttctc ggtctcgtgg
     5101 cgggagaagc ggaacggttc cagcagatcc tgcggctcgt ccataagctc ctttgctacc
     5161 agcgcaccga ccccgatcat attgtacccg tggttggagt cggcgattgc gtaggcatcg
     5221 tctataacct cctcaccaga gccgcacccc ttggccttcg attctttcgc caggtgccag
     5281 gccgagctca gaccatgaat cccggccccc ataacgacgt atgaggctcg tgtgggtagc
     5341 tgcatcttct catcacctcc gtgtgactcc gcccgcattt cgtctgtgga gaacacacag
     5401 gtcgttatcc tccgcagctc gctcagaata acccaactat ctcgccgttc tcatctacgt
     5461 ctatgccttc ggctgcggga gaagcgctga gccccggcat cgtgcgcata tccccgcaga
     5521 tagggtagac gaacccggcc cccactgacg ccctgacctc cctgaccggg agcgtccagc
     5581 ccgtaggagc ccccttgagc gagggatcgg aggatatgga tagatgggtc ttggccatgc
     5641 agatgggcaa ctcgccgaac ccgatcttct gataggagtc gagttggcga cttgcgatca
     5701 ggtcgtactc aacacctctg gccccgtata cctccttggc gatggtctcg atcttctccc
     5761 tcaaaggagc ttcatcaggg tagagaaacg cgaactcggt cggctcttcg gcggcctccg
     5821 cgaccgcctc ggcgagttcc acagctccag ccccgccatc caagaagtgg ctgcacacgg
     5881 ccacccgtgc gcccatctct ccggcgatct cgcggatggc gtcgtattcc gtctcgtggt
     5941 ctgtagggaa ggcgttgatc gccaccaccg gcgagacacc gtgaaggcgg atgttctcta
     6001 tctgtttctt caggttggct gcaccagcat agacgtcatc ggggttctcc ttttgcatct
     6061 cctccgggag gggcttgcct gcccttagct catagcggcc cgagtggacc ttgagcgccc
     6121 gtacagtggc caccagaacc gccgcgtcgg gctcgagacc cgagtagcgg cacttgatgt
     6181 tgaagaagcg ctctgcgccc atgtccgcac cgaagcctgc ctccgttatg agaaagtcac
     6241 cgccgcggat gccgatcaga tccgccacga ttgaggagtt gcctgtggcg atgttaccga
     6301 aggggccggc gtggaccagg gcaggcgtat tctccagagt ctgcatgagg ttgggtttta
     6361 tggcctcttt catgatgacc gtcatgacgc ctgcgcccat gaggtcctct gcggtcacgg
     6421 ggtttccgtc ggcgtcgcta ccgatcacaa tccgcccgaa gcggctgcgc atatcgtgaa
     6481 gcgaggtgga gagcgagagg atcgccatcg cctccgaggc cgccgtgatg tcgaagccgg
     6541 tctggcgcgg gacgccgtcg gtgtgggcgc ccagccccac gactatattc cgcaaggctc
     6601 gatcgtttac gtccatcacc cgtcgccagg taatgctctg caactcgagg tcgagggcgt
     6661 tgccctggta gaggtggttg tcgatcatgg cggcgagctg gttgtgggcc tcggtgaccg
     6721 cgtggatgtc accggtgagg tgcagattga gcgcatccat cggcacgacc tggctgtagc
     6781 cgccgccggc ggcgccgccc ttgataccga acacagggcc catcgaggcc tggcgcaccg
     6841 ccaccgtggc cgtcttgccg atgtgcttga aggcctgccc gaggcctacg gtggtcgtcg
     6901 tcttgccctc acccaaaggg gttggcgtta tggcggagac caccacgtat ttggccttcg
     6961 gcc
//
LOCUS       DATMJA010000205         5333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_501486, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000205 DATMJA010000000
VERSION     DATMJA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501486"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1362)
                     /locus_tag="VNT12_04835"
     CDS             complement(232..1362)
                     /locus_tag="VNT12_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HWI45479.1"
                     /translation="MGEGSRMGFVAGLEDVVAAETRLSSVDGEAGELTIAGFPVEELA
                     GRASFEETVYLLWHDVLPDSGQLADFREELAGLRELSLATLDLLWAVAAEKVPVMDAL
                     RMAAGTVSPGTDDKEALALVARLPTIVAAYSRMLEGEEPVAPDPGLGHAANYLFMLTG
                     EVPGTEFTRALDTYLSTVSDHGMNASTFAARVIVATRSDFVSAVVGAIGALKGPLHGG
                     APGPALDVVFEIGDASRTEAYLREKLGRGERLMGFGHRIYRVRDPRADVLAAAAERLY
                     TTDGDRDLYELALEVEQAALALLAEHKPGRNLQTNVEFYTALLLHGLGLPTQLFTPTF
                     AAGRVAGWTAHCTEQRALDRLIRPQSVYVGSRDRSWVPPEER"
     gene            1516..2739
                     /locus_tag="VNT12_04840"
     CDS             1516..2739
                     /locus_tag="VNT12_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWI45480.1"
                     /translation="MREERYLSAGRAAEELGVSLATLYAYVSRGMVRSEAVEGKRRNR
                     RYRAEDVRRLKERKERRRDPDGVVEGALHWGTPVLESGITLIDGGGLYYRGRDVVDLA
                     GEKSIEEVAALIWTGNEAMAPALFPPEMSGPSRLIRNVVDSVTGLTPVEVFQVLLPVA
                     VAEDPAAYDLRPGAVARTGARILRLVTDVAAGESVPGLAGTLQRGWSPDDPGAADLLD
                     SALVFCVDHELPVSTFAARCVASSEAAPYAVVVAGLAALGGVKHGGEIELVEAFLREV
                     DAAEDARAVISGRLRRGERIPGFGHSLYPEGDPRGAGLLSLTAGAYPESIAVALSDSV
                     AGEMLRLMDEHPTVDFALATVARTLELPPGGAVALFALGRTVGWIGHAIEQYRSGSLI
                     RPRARYVGEQPREAL"
     gene            complement(2736..3458)
                     /locus_tag="VNT12_04845"
     CDS             complement(2736..3458)
                     /locus_tag="VNT12_04845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWI45481.1"
                     /translation="MADPQGTLLSVVLLDAAVAGIVVGIFWLAVSLLRRMGKKIGYSL
                     APLGFSRPRGGALAGIGAGMAVGIGAFLLSMIVTPLSVLVLDRLGYSTESTVQQPFME
                     GLVGWVQERPGVAVPAIVLVVVLFGPAVEELVFRGVVFNGLYRLGALVYTRTIGKEHS
                     IRSPSKTIFISSALASSVLFALLHLEPVLLPVLLILAITLCALFQRTGSLLPPFVAHA
                     TFNSVAAVLIILRGLNVLNIPI"
     gene            complement(3451..3942)
                     /locus_tag="VNT12_04850"
     CDS             complement(3451..3942)
                     /locus_tag="VNT12_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI45482.1"
                     /translation="MGKDRAVIGMDFGVRVAAVVEREGALLLVRHQKPDRDPYWVLPG
                     GRLEPGETIPECAGRELAEETGLTAGFSGVLYMSEFLREGRHTVDVVARMELEGDGEA
                     SLGSDPEVEPGTEPTLREIRWVSIDELREIELLPASIKGRLLKDAPDGWTPDNVYVGS
                     TDG"
     gene            complement(3968..>5333)
                     /locus_tag="VNT12_04855"
     CDS             complement(3968..>5333)
                     /locus_tag="VNT12_04855"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWI45483.1"
                     /translation="VYSGKDGEYVKAFGDRVSEGARLLDRRAGLEQEFFLVENSGHPS
                     ERADVFLKRCREESGGPVCFAPEFVLGLIEVNTPPVYTLYDLEREYVQNLRTALRTAR
                     SLGLRLYSLGTYPLPLKPVVRGGLDYRVQVNTVGPERFEDAGRCAGTHLHLELQAGTV
                     DKGAGISATASAAAREEVSNIYNLATALDPALVALTRSCPFFEGRKTGLAVRTVHYRG
                     SASFGWEGVYKDLPQVGALLPYAEDAEHLIRQQFDRYKAWLSAMDRAGVQRRYFAEAG
                     GDLLRPAWNPVRLNRQGTLELRSMDSNYPEVTLTVAAIILRAADRVRRDGLEVLPDDG
                     VLAFEVTGDVLRVPGFSHLGEELLHAAVTGGAGDETVAAYLDSILEFAGMEDERLAVL
                     FHHRHTTGSYPTTEAGILEAYDGHLSEDEGLRLVLEACDELENQVSQLSRSQRQEEEP
                     ADVS"
BASE COUNT          953 a         1727 c         1692 g          961 t
ORIGIN      
        1 gcacgcgaaa ggaggtgatc cagatagacg catcgagtag tagtcgcggg tatcttctgg
       61 gagaggtggc tctgacctag gcccaccaaa acacccggcg ggatcctggg ttactttatc
      121 cgcctcccat gtagtgaagg cggacgtgct gaaagacggg atacctgcgt aacgctcaga
      181 agggtacggc ccccggagtt ctccgggggt cgtttttcgt atctccccgc actaccgttc
      241 ttcgggtggc acccagcttc tgtccctgct ccccacgtac acggactgcg gcctgatgag
      301 acggtcgagt gccctctgtt cagtgcaatg cgccgtccat cccgctaccc tccccgctgc
      361 gaaggtgggg gtgaagagct gcgtgggcaa cccaagcccg tgaagcagta gcgctgtgta
      421 gaactctacg ttggtctgca ggttcctgcc tggcttgtgc tccgcgagca gtgcgagcgc
      481 agcctgttcc acctcgagcg ccagctcgta gagatctctg tccccgtcgg tggtgtacag
      541 gcgctcggcg gcggcggcga gcacgtccgc cctcgggtcg cgtacacggt agattctgtg
      601 cccgaaaccc atgagccgct cgccgcgccc gagtttctcc cgcaggtaag cctcggtcct
      661 gctcgcatcc ccgatctcga acaccacgtc cagcgcgggc cctggcgccc cgccgtgcaa
      721 cggtcccttg agcgccccga tcgccccgac cacagccgag acgaagtccg agcgtgtagc
      781 gacgatgacc ctcgctgcga aggtggaggc gttcatgcca tgatcggaca cagtactgag
      841 gtacgtatcg agggcccgcg tgaactctgt gccaggcact tcgcccgtga gcatgaacaa
      901 atagttggcc gcatggccca gcccaggatc aggggccacg ggctcctcgc cctcgagcat
      961 gcgggagtat gccgccacaa tggtgggcag acgcgccacc agcgctagag cctccttatc
     1021 gtccgtgcca gggctcacgg tccctgccgc catgcgcagc gcatccatga ccggcacctt
     1081 ctcagcagcc acggcccaca gcagatccaa cgtagccagc gacagctccc gcagccccgc
     1141 gagctcctct cgaaaatccg cgagttgccc ggaatcaggc aagacgtcgt gccagagcag
     1201 gtagacggtc tcctcgaaag aagcccgccc cgccaactcc tcgaccggga aacccgcgat
     1261 ggtcaactcc ccggcctctc cgtccacgct gctcaggcgg gtctcggccg ccaccacgtc
     1321 ctccaacccc gcaacgaaac ccatccgaga accctcgccc atgtcggtcc tttcatgctt
     1381 gacccggatc cccgagccgc cgtcctagtc ctcaatcgta actctctata gaacatgtat
     1441 caatattgat gtgataatca atatatagta gggttgcaaa ggtatggtcg tacaccctgg
     1501 cgtggaggct ttcggatgag ggaggagcgg tatctcagcg ccgggcgggc tgcggaggag
     1561 ttgggggtca gtctggcgac tttgtatgcg tacgtgagca gggggatggt acgttccgag
     1621 gcggtcgagg ggaagaggcg caacaggcgt tacagggctg aggacgtaag gaggctcaag
     1681 gagaggaagg agcgcaggcg ggaccctgac ggggttgtgg agggtgctct gcactggggc
     1741 acgcctgtgc tggagtcggg gatcaccctg atcgacggtg gcggactgta ctatagggga
     1801 cgcgacgtcg tggatctcgc cggggagaag agcatcgagg aggttgcggc cttgatttgg
     1861 acgggcaacg aggccatggc cccggcactc ttcccacccg agatgtccgg accctcccgg
     1921 cttatccgga acgtcgtaga cagcgtgacc ggactaacgc ctgtcgaggt cttccaggtc
     1981 ctgctccctg tggcggtcgc cgaggacccg gccgcctacg acctgcgtcc tggcgccgtc
     2041 gcgcgcaccg gcgccaggat actccgcctg gtgacggacg tggccgccgg agaatcggtc
     2101 cctgggcttg ctgggacgct acaacgtggc tggagtccgg acgatccagg ggctgccgac
     2161 cttctcgact ccgcgctcgt cttttgtgtg gaccacgagt tacccgtctc gacctttgct
     2221 gccaggtgtg tcgcctcgtc cgaggccgcg ccctatgccg ttgttgtggc cggacttgcg
     2281 gcgctcgggg gcgtcaagca cgggggggag atcgagctcg tcgaggcctt tttgcgggag
     2341 gtggatgccg cggaggacgc gcgggcggtg atctccggcc gtctcaggcg gggggagcgt
     2401 atccctgggt tcgggcactc gctttatcct gaaggagacc ctcgtggtgc cgggttgctt
     2461 agcctgacgg ccggggccta tcccgagagt atcgccgtcg cgctttccga tagtgttgcc
     2521 ggagagatgc tgcgattgat ggacgagcat cccaccgtag atttcgcgct ggcgacggtg
     2581 gcgcggacgc tggagttgcc gcctggcggg gccgtcgcgc tcttcgccct cggcaggacc
     2641 gtcggttgga tcggccacgc catagagcag tacaggagcg gctccctcat acgtcccagg
     2701 gccaggtacg tcggagaaca gccgcgcgaa gcgctttaga tcggtatgtt caggacgttc
     2761 aggcctctca ggatgatcag cacggcggcg acggagttga acgtcgcgtg cgcgacgaac
     2821 gggggaagga ggctacccgt acgctggaag agagcacaca gggttatcgc caggatcaag
     2881 aggacgggca ggagaaccgg ttccaggtgc agcagggcga acaggacgga agatgcgagc
     2941 gcggaagaga tgaagatcgt cttgctggga gaccttatgg aatgctcctt cccaatggtc
     3001 ctggtgtaga caagggctcc cagccggtag aggccgttga aaacaacacc cctgaatacc
     3061 agttcctcca ctgccggacc gaagagcacg acgaccagga ctatcgccgg tacggccacg
     3121 ccgggacgct cctgtaccca gccaacgagc ccttccataa acggttgctg gaccgtcgat
     3181 tccgtagagt agccgagccg gtcgaggacg aggacgctga gaggggttac gatcatacta
     3241 aggaggaagg ctccgatgcc gacggccatc ccggcgccga tccccgccag ggcacccccc
     3301 cgcggacgtg agaacccgag gggtgccagg gagtagccta tcttcttgcc catgcgacgc
     3361 agcaacgaga cggccagcca gaatatcccg accacgatcc ccgcgacggc cgcatccaac
     3421 aaaacgaccg agagcaacgt gccctgcggg tcagccatct gtgcttccaa cgtagacgtt
     3481 atccggggtc catccgtcgg gggcgtcctt caggagcctt ccctttatgg aggccggcag
     3541 aagctcgatc tcccgcagct catcgatgct cacccagcgt atctctctaa gcgtcggctc
     3601 ggtaccaggc tcgacttcgg ggtcgctccc aaggctcgcc tccccgtctc cttccagctc
     3661 catacgggcg acgacgtcga ccgtatggcg tccctccctc aggaactcgc tcatgtagag
     3721 gacgcctgag aagccggcag tgaggcccgt ctcctcggcg agttcgcggc ctgcgcactc
     3781 cggtattgtc tcgcccggct cgagtctgcc tcctgggagc acccagtacg ggtccctgtc
     3841 tggtttctga tgacgtacca ggagcagggc tccctcccgc tccacgactg ctgcgacccg
     3901 caccccgaag tccataccga taactgcacg atctttgccc atacggcgag tatacccgcc
     3961 gggagggcta cgagacgtcc gccggctctt cctcctggcg ctgagaccgg ctcagttgcg
     4021 agacctggtt ctccaactcg tcgcaggctt cgagcaccag gcgcagacct tcgtcttcgg
     4081 agagatgtcc gtcgtaggct tcgaggatac cggcctcggt ggttgggtag ctgccggtag
     4141 tatggcggtg gtgaaagagg acggcgagcc tttcgtcctc catcccggcg aactccagga
     4201 tcgagtccag gtaggcggct acggtctcgt cgcccgctcc tccggtaacc gccgcgtgga
     4261 gcagctcctc gccgagatga ctgaagccag gcaccctcag cacatctccc gtgacctcga
     4321 aagccagcac cccgtcgtcc ggcaatactt ccaggccatc ccgcctgacg cggtccgcgg
     4381 cgcggaggat aatggccgcc acggtcagcg ttacctcggg gtagttgctg tccatgctgc
     4441 gcaactctag cgttccctgg cggttgaggc gcacagggtt ccaggccggt ctgagcaggt
     4501 ccccgcccgc ctcggcgaag tagcgtctct gtacgccggc ccggtccata gcgctgagcc
     4561 aggccttgta gcggtcgaac tgctggcgga tcaggtgttc agcgtcttcg gcgtagggta
     4621 gcaaggcgcc tacctggggc agatccttat acactccttc ccacccgaag ctcgcgctgc
     4681 ctctgtaatg gacggtgcga acggccagcc ccgtcttccg cccctcgaaa aatggacacg
     4741 agcgggtgag ggcaaccagg gcggggtcga gagctgtcgc aaggttgtag atgttcgaga
     4801 cttcctccct ggcggcggct gacgcggtgg cggagatgcc cgctcccttg tcgaccgtcc
     4861 ctgcctgcaa ttccagatgc aggtgcgtac ccgcgcatct cccggcgtct tcgaatctct
     4921 ccgggccgac agtgttcacc tggaccctgt aatccaaccc gccgcggacg acgggcttca
     4981 gaggcaaagg ataggtgccg agcgagtaga gcctgagacc cagagatctc gccgtgcgta
     5041 gggcagtccg caggttctgc acgtactcgc gctcgaggtc atagagagtg tagaccggag
     5101 gcgtgttcac ctctatcaaa cccagcacga actcgggagc gaagcacaca ggaccgccgg
     5161 actcctcgcg gcagcgcttc aggaacacgt cggccctctc cgatggatgc cctgagttct
     5221 cgaccaggaa gaattcctgc tcgagcccgg cccgtcgatc caacaacctg gccccttcgc
     5281 tcaccctgtc cccgaatgcc ttgacgtatt ctccatcttt tccggagtag acg
//
LOCUS       DATMJA010000206         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_205530, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000206 DATMJA010000000
VERSION     DATMJA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205530"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VNT12_04860"
     CDS             complement(<1..673)
                     /locus_tag="VNT12_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWI45484.1"
                     /translation="MLDFLRNLLDPLFNLMGGTLSTFHGWGASWWLAIVMLTIVVRTL
                     LFPITYRQVKSMRNMQDLKPDMDRIRAEYKDDVQKQREEMAKLYQERQVNPLGGCLPI
                     FIQLPIFLVLYYTIRHFDTLESFRTGGLLWFQNLTQPDHLFILPVLYMLTMMASQELT
                     IRNTATQQKQVMRFLPIIFGVFLARFPAGLFVYWVTSNLITFAQNYVIYYVLPHKPVD
                     TEEDKS"
     gene            complement(743..1072)
                     /locus_tag="VNT12_04865"
     CDS             complement(743..1072)
                     /locus_tag="VNT12_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45485.1"
                     /translation="MRRISSPAFGLVLFGLLVNFFSREEIYANLEYGFPMLALFGAAG
                     CIGQLTASISRRSGTLASACCIPIGVLRARVIRDGVRGETSDLPVLIALLFCPLVVLL
                     AGLGGWV"
     gene            1319..>2206
                     /locus_tag="VNT12_04870"
     CDS             1319..>2206
                     /locus_tag="VNT12_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWI45486.1"
                     /translation="MAVETETIRNVCLIGHRGSGKTSIAEGMIGLASGRSGPTSGVLD
                     YTDEETERGMTLGMSVANLEWKGRQVNVLDTPGDGGFVGDAFIAQRVADCAILVVHAQ
                     DPIQVVTERLWRRGEKEGIPHVIVVNHLDRERTDFGTVVEQLRGRFGQAVVPLNLPIG
                     RENGLEGIYGLLSGTSFVDGEQSAEIPEGMEDEVDTSKTQLFEAIAESDDTLLEKYLE
                     GEEITTEEAFEGIRKGIAEGLIIPVLAASAERMIGLDRLLDVIAGSAPSPSDLERWIT
                     EKGDEVPSDPEGPFAAYAFK"
BASE COUNT          463 a          602 c          744 g          397 t
ORIGIN      
        1 gggatttgtc ttcttcggtg tctacgggct tgtgcggaag gacgtagtag atcacgtagt
       61 tctgggcgaa ggtaatgagg ttggaggtta cccagtagac gaagagccct gcggggaatc
      121 tggcgaggaa gacgccgaag attatgggca gaaagcgcat cacctgcttc tgttgcgtgg
      181 ccgtgttcct tatggtcagc tcctgagagg ccatcatggt cagcatgtag aggacgggga
      241 ggatgaagag gtggtcgggc tgggtcaggt tctggaacca caggaggccg ccggttctaa
      301 agctttccag cgtgtcgaag tgcctgatcg tgtagtacag gactaggaag atggggagct
      361 gtataaagat gggtaggcat ccgccgagtg ggttgacctg ccgctcctgg tagagcttcg
      421 ccatctcctc ccgctgcttc tggacgtcgt ctttgtactc ggcccggatt ctgtccatgt
      481 ccggtttcag atcctgcatg ttgcgcatgc tcttgacctg gcggtaggtg atcgggaaga
      541 gcagtgtacg cacgacgatg gtgagcatga cgatggccag ccaccacgac gcaccccaac
      601 cgtggaaggt cgagagtgtt ccccccatga ggttgaatag tgggtctaga aggttcctca
      661 aaaagtcgag cacgtttcgt cgtttccttt atctagcagg gtgtcgtgcc gcgcggagcc
      721 ccgcggggac ggggcgctac ccttataccc agccgccgag gccggcgagg agaacgacga
      781 gcgggcagaa aaggagtgct atgaggacgg gaaggtcgct cgtttcgcca cgaacgccat
      841 cccttatgac cctcgcccgc agaaccccta tggggatgca gcaggcggag gccagggtgc
      901 ccgagcgtcg agagatgctg gccgtgagct ggccgatgca gcctgccgcg ccgaacagcg
      961 cgagcatcgg aaagccatat tcgaggttgg cgtagatttc ttccctggag aagaagttga
     1021 cgagcaaccc gaataacacg agcccgaagg cgggggagga gatcctccgc aacgcatccg
     1081 ttaagactgc cgcacgcctt ctctcgaact tactgctcag accagatctc ctacaaagat
     1141 tttgtcggcg acgttactcg tcgcccaagc cctgagtcta gcacttttcc ggccgctgac
     1201 gatgggagcc agcacgcctt tgtgaatgag acctggcctg cgcgaagggc ctctctgcga
     1261 taagctacat gccccgagcg cggggatgaa gggattgagc tcaagagagg aggaatccgt
     1321 ggctgtagag acggagacca tcaggaacgt gtgcctgatc gggcaccgcg gaagcggcaa
     1381 gacatcgata gccgagggta tgatcgggct ggcttcggga cgaagcggtc cgacgagcgg
     1441 tgtcctcgac tacacggacg aggagaccga gcggggcatg acgctgggca tgagcgtcgc
     1501 caacctcgag tggaaggggc gccaggtcaa tgtgctcgac acccccggag acgggggctt
     1561 cgtcggggac gctttcatag cccagagggt ggccgactgc gcgatcctgg tagtccacgc
     1621 ccaggacccg atccaggtcg tcaccgagcg gctgtggcgc aggggggaga aggaaggcat
     1681 tccccacgta atcgtcgtca accacctcga tagggagcgc acggatttcg ggaccgtagt
     1741 ggagcaactc aggggacgct tcggccaggc tgtggttccg ctgaatttgc ccatcggacg
     1801 cgagaacggc ctcgagggta tctacgggct cctcagcgga acctcctttg tggacggcga
     1861 gcaaagtgca gagatccccg agggcatgga agacgaggta gacacgtcca agacgcagct
     1921 cttcgaggcc atagcagaga gcgacgacac gctgctggag aagtacctcg aaggcgagga
     1981 gatcacgaca gaggaggctt tcgagggaat ccgcaagggg atcgccgagg gcctcatcat
     2041 ccctgtcctc gcggcgagcg cggagcgcat gatcggactc gaccgcctgc tcgacgtgat
     2101 cgccggaagc gccccaagcc cttcggacct ggagcgttgg atcacggaaa agggcgatga
     2161 ggtccctagc gaccctgaag gacccttcgc ggcctacgcg ttcaag
//
LOCUS       DATMJA010000207         3476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_560679, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000207 DATMJA010000000
VERSION     DATMJA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560679"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VNT12_04875"
     CDS             complement(<1..283)
                     /locus_tag="VNT12_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45487.1"
                     /translation="MRVSGPIRTGYFYGLAGVFVALASDLYFLFVDPADTPGWVLAAI
                     AQYRTRLALALFLFLAILAALRVRPTRLDPGVPYRSLLVRDGALAATVVA"
     gene            complement(356..1195)
                     /locus_tag="VNT12_04880"
     CDS             complement(356..1195)
                     /locus_tag="VNT12_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI45488.1"
                     /translation="MLAVSASAIFIRLAEAPAIAVAFWRCALGAAVLLPPALVRKDSF
                     PRGRALYVGIASGVALGAHFGFWISSLDYTSVAASVVLVSTQPVFVAVLAYLLFGERT
                     TPLSFLGIVVAIAGTAVIAFDRTVGSAAVLGNVLALVGAVTVAVYVLIGRFSRTTGGV
                     GVLPYSIVVYSAAAATLLPVALGFEVRLWGYSGETWFWLAAITAGPQLMGHTVFNWAL
                     RYVQASIISGTILAEPVVAALLAWFVLSERPGLLTIVGGAIVLAGLYCLLKGRSAAGE
                     PDP"
     gene            complement(1204..1806)
                     /locus_tag="VNT12_04885"
     CDS             complement(1204..1806)
                     /locus_tag="VNT12_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWI45489.1"
                     /translation="MKGPFRGGILGQLGELLRVYIGRPSISVPDGIRARPRVAVVLGA
                     QVLPGGRPSPTLEARTRHAGELYIRGMADHLVPTGGEGERPPSEAVVMSGILRKMGVP
                     EAAILREDTARSTWESAVRVAEIVHHRGIGEVLVVTDPLHCVRTVASFGRVGLLAVAE
                     PVYSSPMWRKKWSRRGQFVRESGALVWYRIRHGVGLLSRP"
     gene            complement(1803..2399)
                     /locus_tag="VNT12_04890"
     CDS             complement(1803..2399)
                     /locus_tag="VNT12_04890"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWI45490.1"
                     /translation="MSRPASGIILAGGASRRMGREKLSLEVEGVPLIERVRDALTDCC
                     REVLVVGGGGVRLEGVKYITGERPGGLGPLAGMEAGLASARYPLTFVAAGDMPFLTQS
                     IVSYLLGRLARGGVLAVVPRYRGRIHPLCAAYARTLLPRLGTALDGGARAVHGFLEGV
                     GEVEYVGEELRRFGDPGLLLMNVNSPRDLELARREARR"
     gene            complement(2396..3385)
                     /locus_tag="VNT12_04895"
     CDS             complement(2396..3385)
                     /locus_tag="VNT12_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HWI45491.1"
                     /translation="MSLSGPLIWLGILQAVIYLLFIRAIDLYEREPLRYVIPVFIWGF
                     AVATTVSLVFNTLFQLTLSSVTSMKTASFFTAVVEAPVVEECSKGAALLLIYFVAYLA
                     RRRSGLVEFAGVMDGIVYGSAVGFGFSIAEDLLYGLQFGPETYIVRRIFGGFAHASFT
                     SLTGIGIGLIPLVHNSVLKVILPVLGLFGAILLHAAFNFTATVFGPVGYLVLFCVILV
                     YIVIIFVWLAIERRAIRDELREEVQAGTITSQEYDIIPSYFRRTGYYLGLLFTGRLRT
                     WFRARKLHATAVDLALAKRLARRTGSPGSIDRVRTLRRKILDSREGAAVRAFS"
BASE COUNT          732 a         1149 c         1072 g          523 t
ORIGIN      
        1 cagcgaccac cgtcgccgcc agggcgccgt cccggaccag cagtgatctg tagggcactc
       61 cgggatcgag gcgcgtgggg cgtaccctga gtgccgccag tatggccagg aacaggaaca
      121 gagcaagggc cagtcgcgtc ctgtactgcg ctatcgcggc aaggacccaa ccaggcgtat
      181 cggcagggtc gacgaagagg aaatacaggt ccgaggcgag cgcgacgaac acacccgcga
      241 gcccgtagaa gtaacccgtc ctgatcggtc ccgaaactct cactgggtaa ccgtacatac
      301 aggggaggat acagcatcac ccagggacgc tctgtgatct agaatcccac gcctgtcagg
      361 ggtcgggctc gcccgcggcc gaacgcccct tgaggagaca gtacaggccg gcgagcacga
      421 tcgcgcctcc tacaatcgtc agcaggcctg gtctctcgga gaggacgaac caggccagaa
      481 gcgcagcgac gacgggctcg gccaggatgg tgcccgagat gatggaggcc tgcacgtagc
      541 gcagggccca attgaagacc gtgtggccca taagctgcgg ccctgcggtg atcgccgcca
      601 gccagaacca ggtctctccg gagtaacccc agagacggac ctcgaagccg agggcgacgg
      661 gcagcaacgt cgccgctgcc gccgagtaca cgactatgga ataggggagc acaccgacgc
      721 ccccggtggt gcgggagaag cgaccgatca ggacgtacac agccaccgtt acggccccga
      781 cgagggcgag cacgttcccc aacacggcgg ccgatcctac ggtcctgtcg aaggcgatga
      841 cggccgtgcc cgctatggcg accacgatgc caaggaacga tagcggggtg gtgcgctctc
      901 cgaagagtaa gtatgcgagg acagccacga agacgggctg ggtggacacg agaacgacgc
      961 tcgcggccac gctcgtatag tcgagcgaag agatccagaa tccgaaatgg gccccgagcg
     1021 ccactcctga ggcaataccc acgtagagtg cacggcccct cgggaagctg tccttcctga
     1081 cgagcgccgg cggcaggagc acagcggccc caagggcaca cctccagaac gcaacggcaa
     1141 tggcaggggc ctcggccagc cggataaaga tggcagacgc cgatactgcg agcaccccta
     1201 cggctacggc cgggagagca accctactcc atgccttatc ctgtaccaga ccagagcccc
     1261 actttccctg acgaactgcc ctctacggga ccacttcttg cgccacatcg ggctactata
     1321 caccggctcc gccacagcga gcaggcctac cctgccgaat gaagcgaccg tcctgacgca
     1381 gtgcaatggg tctgtgacca caaggacctc gccgatgccc ctgtggtgta cgatctcagc
     1441 caccctcacg gcggattccc aggtgctcct ggcagtatcc tcgcgcagga tcgccgcctc
     1501 gggcacaccc atcttccgca ggatgcccga catcacgaca gcctcactgg gcggacgttc
     1561 gccctcaccg ccggttggta cgaggtgatc agccatgccc cgaatgtaca actcgcccgc
     1621 gtgacgcgtc ctggcctcca gcgtagggct cggcctccct cctggcagta cctgcgcccc
     1681 gagaaccacg gcgacccggg gtctggctct tatgccgtca gggaccgaga tcgatggccg
     1741 acctatgtac accctgagga gttcacccag ttgcccgagg atgcctcccc gaaacggacc
     1801 cttcaacgcc gggcctcgcg ccttgccagc tccagatccc gtggcgagtt gacgttcatc
     1861 agcaggaggc cggggtcccc gaaccgccgc aattcctccc cgacgtactc gacctcgcca
     1921 accccttcca gaaacccgtg cacagcccgc gccccgccgt cgagcgccgt cccaagacgt
     1981 ggtagcaacg tcctggcgta ggcggcgcag agcgggtgga ttctgccccg gtatcgtggg
     2041 accacagcaa gaacgccgcc cctcgcgaga cgcccaagca ggtaacttac tatgctctgc
     2101 gtcaggaagg gcatgtcacc ggctgcgacg aaggtaagcg ggtaacgtgc ggaggcgagg
     2161 ccggcctcca taccggccag tggtccaagc ccgccagggc gctcgccggt gatgtatttg
     2221 actccttcga gcctgacacc gccacctccg actacgagca cctcccggca gcagtctgtg
     2281 agcgcgtctc tgacacgttc gatcagtggc accccctcta cctccaggga tagcttctcg
     2341 cgccccatcc tgcggctggc gccgccggcc aggattatgc ctgaggccgg gcggctcagg
     2401 agaacgcccg gacggccgct ccctctctcg agtcgaggat cttgcggcgg agcgtcctga
     2461 cgcggtctat gctgcctggg ctacccgtgc ggcgggcaag gcgttttgcg agggccaggt
     2521 ctacggcggt agcatgcagt ttgcgggccc tgaaccatgt gcgcagcctt cccgtaaaga
     2581 gtagcccaag gtagtagcct gtccttcgga agtagctggg aattatgtcg tattcttgcg
     2641 aggttatcgt cccggcctgg acctcctccc tgagttcgtc ccggatagcg cgccgctcga
     2701 tggcgagcca cacgaaaatg atgacgatgt aaacgaggat tacgcagaac aacaccaggt
     2761 agcctacagg cccgaacacc gtggctgtaa agttgaaggc cgcgtgcaac agtatcgccc
     2821 caaagaggcc gaggacgggc aggatgacct tgagaacgct gttgtgcacc aaggggatga
     2881 ggccgatacc gatccccgta agcgaagtga aagacgcgtg ggcgaagccg ccgaagatcc
     2941 tgcggacgat atacgtctcc ggaccgaact gtagaccgta gagcaggtcc tcggcgatgg
     3001 agaagccgaa cccaacagcc gagccgtaga cgataccgtc catcacaccg gcgaactcga
     3061 ccaacccgct cctgcgccgc gcgaggtagg ctacaaagta gatcaagagc agtgcggccc
     3121 ccttggagca ctcctccacc acgggcgcct cgacgaccgc cgtgaaaaag cttgcggtct
     3181 tcatgctcgt caccgagcta agcgtgagct ggaagagcgt gttgaagacc agtgagaccg
     3241 tcgtcgccac ggcgaacccc cagatgaaga caggtatcac gtagcgcagc ggctcccgct
     3301 cgtagagatc tatcgcccgg ataaagagga ggtagatcac ggcttgcagg atgccgagcc
     3361 agatcagtgg gccgctcagg ctcacgaggc tggccttccg gagtatgaca tggaccgcag
     3421 tatacccgct cctgataaaa tacctctgtc cttaccgaaa ggagaggatt ggacag
//
LOCUS       DATMJA010000208         2766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_679371, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000208 DATMJA010000000
VERSION     DATMJA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679371"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VNT12_04900"
     CDS             complement(<1..792)
                     /locus_tag="VNT12_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HWI45492.1"
                     /translation="MRQAVGIENGDAPALLVVGHGSRDPRGAREFHDLVGLVRRRNPS
                     LTVEGGFIELARPPISECVDRLAESGIRNVAAVPLMLLAAGHAKDDIPATLVREKMGH
                     PEMSFSYGRALGIRPELLELMEERISTVVLEEEKEETAVLIVGRGSSDPDANSDLCKI
                     ARLYYEGRHYPVVESAYVSMTTPDVANGLDRCLKLGAMHVVVFSYFLFTGVLEERIRG
                     QSRAFAEANPGVEVRYAGYFGPDPRLADLVVERYTEALRGDIRMNC"
     gene            complement(794..1378)
                     /locus_tag="VNT12_04905"
     CDS             complement(794..1378)
                     /locus_tag="VNT12_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HWI45493.1"
                     /translation="MRRVHPIEMESYRILRGMVDLSHLELLSRAVAERVIHASADLDY
                     AESLLLDEAALENGISALRRGKPVVTDVGMVAAGITGRRTLCLVSDPRAQALSEQLGI
                     TRSAAGFRVATEEVGKGAVWVVGNAPTALSELLALDVAPSLVIGLPVGFVGAAESKSA
                     LVASGLPAVANRGPKGGSAVAAAALNALIFYEEG"
     gene            complement(1375..1578)
                     /locus_tag="VNT12_04910"
     CDS             complement(1375..1578)
                     /locus_tag="VNT12_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWI45494.1"
                     /translation="MKAVFAVTEACAGCGACLKTCPEKAIRPAPRDYGSPLLVLEDRC
                     SGCAECAEVCPVSACVEVLLEAE"
     gene            complement(1575..>2766)
                     /gene="cobJ"
                     /locus_tag="VNT12_04915"
     CDS             complement(1575..>2766)
                     /gene="cobJ"
                     /locus_tag="VNT12_04915"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HWI45495.1"
                     /translation="PNNVLGTDEWKAPMIVITDRLVETPRPAAVYRPPSLIAGVGCSR
                     GVGAEEILDLLRRSLAEAGLTEKSVAALASIDVKRDEAGLLEAAQMLDVTIHFHSAQA
                     LRAVEAPNPSELVREAVGTPSVAEAAVVSSGAELVLEKRKSANVTVAVGRLPVRGRLA
                     LVSLGPGEDTLLPPLAREALATSELVVGLEQYVERIRHLLRPGTRVLTLTLGNEVERA
                     QLALSEARAGGSVALVSSGDVGIYGMASPALELADEDVDVVVVPGVTAAQAAASLLGS
                     PLGHDHCSVSLSDLLTPWGVIQDRVRAAAEGDFVISLYNPRSKGRDWQLGKVKEILLR
                     YRPPDTPVGIVKDAYRPTQQIILTDLASLRPEDVDMLTIVLVGSSQTRVVAGRMVTPR
                     GYLS"
BASE COUNT          509 a          924 c          851 g          482 t
ORIGIN      
        1 gcagttcatg cgaatgtccc ctctcagggc ctctgtataa cgctcgacca caaggtcagc
       61 cagcctcgga tcaggcccga agtatcctgc gtagcgcacc tcgacgccgg gattcgcctc
      121 agcaaatgct ctgctctgcc cgcggatacg ctcctcaagg acgccggtaa agaggaagta
      181 ggagaagact acgacgtgca tggcgccgag cttgaggcag cgatccagcc cgttcgcgac
      241 atcgggcgtg gtcatgctga cgtaagcact ttcgactacg gggtaatggc gtccctcata
      301 gtacagacgc gctatcttgc agaggtctga gttggcgtcg gggtcggagg agccgcgacc
      361 gacgatgagg actgctgtct cctccttctc ctcctcgagg acgacggtgg agatcctctc
      421 ttccatcagt tcgagaagct cgggtcttat gccgagggcg cgaccgtagc tgaagctcat
      481 ttcggggtgg cccatcttct cgcgcaccag ggtcgcgggg atgtcgtcct tggcgtggcc
      541 cgcggccagg agcataagcg ggacggccgc gacgttcctt atcccactct cagcgagccg
      601 gtccacgcac tccgagatgg gcggtcgagc gagctcgata aagcctccct cgaccgtcaa
      661 cgatgggttt cttcggcgca ccaggccgac cagatcgtgg aactcgcgtg ctccgcgagg
      721 gtcacggcta ccgtggccta cgaccagcag cgccggggcg tctccgtttt cgataccaac
      781 cgcttgcctc acgtcatccc tcctcgtaaa agatgagagc gttcagagcc gctgctgcga
      841 ccgcagaacc gcctttcggt cccctgttcg cgacggcggg caggccactt gctacgagtg
      901 cactcttcga ctcggcggcc ccgacaaagc cgacgggaag accgatcacg agcgagggag
      961 caacgtcgag cgctagcaac tcggagagcg ccgtcggcgc attcccgacg acccagacgg
     1021 ctcctttgcc gacctcctcc gtagcgaccc tgaagcctgc cgccgaacgc gttatgccga
     1081 gctgttcgga taacgcttgg gcgcgggggt cggagaccaa gcacagcgtc cggcggcctg
     1141 tgatcccggc cgccaccatc cccacatcgg taacgacggg tttgccccgc cgcagcgccg
     1201 agataccatt ttccagggcc gcctcgtcga gtagcaggct ctctgcgtag tccagatccg
     1261 ccgaggcgtg gatgactcgt tccgcgacgg cgcgagagag gagttccaag tgcgagaggt
     1321 cgaccatccc gcgcaggatg cggtagctct ccatctctat gggatggacg cgtctcattc
     1381 ggcctccagc agtacctcga cgcacgccga gaccggacag acctcggcac actcggcgca
     1441 tcccgaacag cggtcctcga ggacaaggag gggcgagccg tagtccctgg gggcaggacg
     1501 gatcgccttc tccgggcagg tctttaggca ggcgccacag ccagcacagg cttccgtcac
     1561 ggcgaagacc gctttcacga caggtatccc cttggggtaa ccatgcgccc ggcgactacc
     1621 ctggtctgag agcttccgac gaggacgatt gtcagcatgt ccacatcttc cgggcgcaga
     1681 gaagcgaggt cggtgaggat gatctgctgt gtcgggcggt aggcgtcctt cactatgccg
     1741 accggcgtat caggaggacg gtacctgagc aggatctcct tcacctttcc gagctgccag
     1801 tcgcggccct tcgagcgcgg gttgtagagc gagatcacga agtctccctc ggccgcggcc
     1861 ctgacccggt cctggatcac accccacggc gtcagcaaat cggagaggct gaccgaacaa
     1921 tggtcgtgcc caagaggtga cccgagtaac gaagccgccg cctgcgcagc tgttacccca
     1981 ggcactacca caacgtccac gtcctcgtcc gccaactcga gcgccggaga cgccatccca
     2041 tagataccca cgtcaccgct cgataccagt gctacgctgc cgcccgcacg ggcctcgctg
     2101 agcgcgagct gagctcgctc cacctcattg ccgagtgtaa gcgtaagcac ccgtgtccca
     2161 ggtcgcagga gatgcctgat gcgttcaacg tactgctcga gcccgacgac gagctccgaa
     2221 gtcgcgagcg cctcgcgggc gagcggtgga aggagggtgt cctcaccagg acccaggcta
     2281 accagggcca gtcttccccg caccgggagc cgcccgacag cgactgtgac attcgccgac
     2341 tttcgtttct cgagtaccag ctctgcaccg gaggagacta ccgcggcctc ggcgacgctc
     2401 ggcgtcccta ccgcctctcg caccagctcc gacgggttgg gtgcttcaac cgcgcggagc
     2461 gcttgggctg aatggaagtg gatcgtgacg tccagcatct gtgcggcctc gagcaacccc
     2521 gcctcgtcgc gcttcacgtc gatgctggcg agggcagcga cactcttctc tgtcaaacca
     2581 gcttccgcca gggagcgacg aagtaggtcg agtatctctt ccgcacccac tcctctacta
     2641 caaccaacac cggcgatgag cgagggtgga cggtagacgg cagcaggacg gggcgtctcc
     2701 acgagcctgt cggtgatgac aatcatcggc gccttccatt cgtccgtccc gaggacgtta
     2761 ttgggt
//
LOCUS       DATMJA010000209         5801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_264680, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000209 DATMJA010000000
VERSION     DATMJA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264680"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2555
                     /gene="carB"
                     /locus_tag="VNT12_04920"
     CDS             <1..2555
                     /gene="carB"
                     /locus_tag="VNT12_04920"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWI45496.1"
                     /translation="LVEKSVAGWKEFELEVMRDLADNVVVICSIENVDPMGVHTGDSI
                     TVAPAQTLSDRQYQTLRTASLRIIREIGVSTGGSNIQFAVDPESDEYYVIEMNPRVSR
                     SSALASKATGFPIAKIAAKLAVGYSLDEIPNDITRATPASFEPALDYVVTKVPRFAFE
                     KFPGAVPRLTTRMHSVGEVMAIGRTFTESLMKAMASLEVDAQDVHAGLDEPSPYRVFA
                     IFDALRAGMDIPEIFQRTSIDPFFIASIARIVAAEGSVCEPFGVEDLRELKRFGLTDE
                     TIATASGTSVEVVRGVRRALGVSPTYKSVDTCAGEFPARTPYYYSTYEQEDEVSRDEN
                     ASVVVLGSGPNRIGQGIEFDYACVHASYALAESGYDAVMINSNPETVSTDYDTSTRLY
                     FEPLTAEHVLEVIRREEPEGVILQFGGQSPLKLAHELEAAGVRILGTSPDAIDLAEDR
                     SRFGRLLAELGIPHPRFGTASTAEEAREVARELGYPVVVRPSYVLGGRRMEIVYNDDD
                     LDLYLRTSAGAGPGHPTLIDKFMEDYTEVDVDAVSDGEEVYVGGIMEHVEEAGVHSGD
                     SSCVTPPITIHRGLLQRIEDYTRRLALSVGVIGLMNVQFVVRGDDVLVIECNPRASRT
                     VPFISKATGVPLAKIATRVLLGEKLGDMALRASTNGHFSVKAPVFPFDRFADVDPLLG
                     PEMRSTGEAMGIDRTFGGAFAKALTAAGQELPVSGRVYISVANREKRAVVLIARAFAD
                     LGFEISASEGTAEVLKNNGLPVMIVPKIGELGEDVLGLIEAGGVDLIVNTPWGRGPRT
                     DGYLIRRRALMHGVPCITTLAGAAAAIQGIEARVRGGTRRVNSLQGLYAARA"
     gene            2552..3286
                     /locus_tag="VNT12_04925"
     CDS             2552..3286
                     /locus_tag="VNT12_04925"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HWI45497.1"
                     /translation="MKFHSVKVENREELGSYTLLEYRWTDGPVEPGQFVMARAAGYPA
                     TLDPFLARPFSFYDYDGKTASLLIEVRGRGTALLDLASTIEVTAPLGKGFRIDPGLGR
                     AALLGGGIGVAPLKILDRRLDAVGTPHDIYLGFADARLAAVAREFPGAEVATMDGSAG
                     THGTVLDATDSLEGYGAVYACGPNPMLAAVKRAARENRLCQLSVEERMGCGNGSCNGC
                     VVPVRRGGYIKSCIEGPVFHSDVLAW"
     gene            3286..4185
                     /locus_tag="VNT12_04930"
     CDS             3286..4185
                     /locus_tag="VNT12_04930"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWI45498.1"
                     /translation="MREQKEGVDLAIELCGLPLRTPLMPASGTLSKEALGEVEGVYGA
                     MLPKTITPATRAGNPPPRIAETPAGMVNSIGLQNPGVHGFLQGLDAFDLGIPILVSVA
                     GDTVEEFALLCEILAGDDRVSAVELNLSCPNVECGGLAFCAGPASVEEVVAACRAAVP
                     GKPIMAKLTNEGVVKNARAAESAGADGLTVINTIPALTVDVRARKVLVRGGLSGPAIK
                     PVALRAVYDVSGVVNVPVIGSGGVVSGTDVAEFMLAGATAVQVGTASFVRGPREILDE
                     FSGYLKENGLSAAELTGALRHPS"
     gene            4513..4983
                     /locus_tag="VNT12_04935"
     CDS             4513..4983
                     /locus_tag="VNT12_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45499.1"
                     /translation="MVNWLKSLFAGSKPAGPPQVLRAFASDQPTITQTGIRVENGAWH
                     IDTRAQQTIRLFEVENPAVEHCLLTYRAELKAEGLQGRAFLEMWCRLPGRGEYFSKGY
                     QQAVSGTVDWARYETPFYLKRDQKPDLIKLNVVVEGPGTVWLRNVELLKTPLAS"
BASE COUNT         1113 a         1814 c         1846 g         1028 t
ORIGIN      
        1 ccctagtcga aaaaagcgtc gccggctgga aagagttcga gctggaggtg atgcgcgacc
       61 tcgccgacaa cgtagtcgtc atctgctcca tagaaaacgt agaccccatg ggcgtccaca
      121 ccggcgactc catcaccgtc gccccagccc agaccctatc cgataggcaa taccagaccc
      181 tccgtaccgc atctttacgc ataatacgcg agatcggggt ttccactgga ggatccaaca
      241 tccagtttgc ggtcgaccct gagagtgacg agtactacgt aatcgagatg aacccgcggg
      301 tgagccgctc gagcgctttg gcgagcaagg ctacggggtt cccgatagcg aagattgcgg
      361 ccaagcttgc ggtagggtac tcactcgatg agatcccgaa cgacattacg cgtgcgacgc
      421 ctgcctcgtt cgagcctgcg ctggactacg tggtgaccaa ggtaccccgc ttcgcgttcg
      481 agaagttccc gggagctgtg cccaggctca ccactaggat gcactcggtg ggcgaggtca
      541 tggcgatagg gaggacgttc acggagagcc tgatgaaagc gatggcttcg ctggaggtgg
      601 acgcccagga cgtccatgcc ggcctcgacg agccgagccc gtaccgcgtc ttcgccatct
      661 tcgacgctct gcgtgccggg atggacatac cggagatctt ccagcgtacc tccatagacc
      721 cgttctttat cgcttccata gccaggatcg tggctgctga aggctccgtc tgtgagcctt
      781 tcggcgtcga ggacctgcgc gagctcaaga ggttcggcct taccgacgag accatcgcaa
      841 cggcttcagg gacgtcggtg gaggttgtca ggggcgtgag gcgggctctc ggtgtctcgc
      901 caacctacaa gtccgtcgat acctgcgccg gtgagttccc tgcgcgcacg ccctactact
      961 actccactta tgagcaagag gacgaagtca gccgcgacga gaacgcctca gtcgtcgtcc
     1021 tcggcagcgg ccccaaccgt atcggccagg gcatcgagtt cgactacgcc tgcgtccacg
     1081 ccagttacgc cctcgcggag agcggctacg acgcggtcat gatcaactcc aacccggaga
     1141 cagtctctac cgactacgac acttccacac gcctttactt cgagccgctc accgcagaac
     1201 acgtgctcga ggtcataagg cgggaggaac ccgaaggcgt catcctgcag tttggcggcc
     1261 agagtccgct gaagctggcg catgaactgg aagcggcagg ggtgcgaatt ctcggcacct
     1321 cgcccgacgc catagacctc gcagaagacc gctccaggtt cggacggctt ttggcagagc
     1381 tcggcatccc gcaccccagg ttcgggactg catcgacggc agaggaggca agggaggtgg
     1441 ctcgcgagct cgggtatccg gtcgtcgtga ggccatctta cgtgctcggc gggcgcagga
     1501 tggagatcgt ctacaacgac gatgacctcg atctatacct caggaccagc gctggcgcag
     1561 ggcctggaca ccctaccctc atcgacaagt ttatggagga ctataccgag gttgacgtgg
     1621 atgctgtctc ggacggggag gaggtctacg tcggcggcat catggagcac gtcgaggagg
     1681 ccggtgtaca ttcaggcgac tcctcatgcg tgacgccccc gatcacgatc caccgggggt
     1741 tattgcagag gatcgaagac tacacgagga ggttggccct ctctgtcgga gtcatcgggc
     1801 ttatgaacgt gcagttcgtc gtgaggggtg acgatgtgct cgtgatcgag tgcaaccccc
     1861 gcgcctcgcg tactgtacct ttcatctcca aggcgacggg cgtgccgctg gccaagatag
     1921 cgacccgggt cctcctcgga gagaagctcg gggatatggc tctacgggct tccaccaacg
     1981 ggcatttctc cgtcaaggcg cctgtcttcc cgttcgacag gttcgctgac gtcgacccgc
     2041 tgctcgggcc cgagatgcgc tctaccggtg aggccatggg gatagatcgc accttcggcg
     2101 gggccttcgc gaaggcgcta acggcagcgg ggcaggagct tcccgtctcg gggagggtct
     2161 acatctccgt cgccaacagg gagaagcggg ccgtggtgct aatagccagg gccttcgccg
     2221 acctggggtt cgagatctcc gcgagtgagg ggacggccga ggtgctcaag aacaacgggc
     2281 taccggtcat gatcgtgccc aagatagggg agctcggtga ggatgtactg ggcctcatag
     2341 aagctggtgg ggtagacctg atcgtgaaca ctccctgggg tcgtgggccg cgcactgacg
     2401 gatacctgat ccggcgccgg gctctcatgc acggggtccc ctgtattacc accctcgcag
     2461 gggccgcggc cgccatacag ggcatagaag ccagggtccg tggcggcacc agacgcgtga
     2521 actcgctgca gggcctctac gcggcgagag catgaagttc cacagcgtta aggtcgaaaa
     2581 tcgtgaggaa ctcggctcct acaccctgct cgaataccgc tggaccgatg gacccgtaga
     2641 accggggcag ttcgtgatgg cgagggcagc cggttaccct gcgaccctcg atccattcct
     2701 tgcccgcccc ttctccttct acgactacga cgggaaaacg gcaagcctcc tcatcgaagt
     2761 acggggccga ggtactgcgc tcctggacct cgcatcgacc atagaggtaa cagcccctct
     2821 cgggaagggc ttccgcatcg atccaggcct ggggcgggca gcccttctag gaggcggtat
     2881 cggcgtcgcg ccactcaaga tactggatcg gcgtctcgac gccgtaggaa ctcctcacga
     2941 catctacctc gggttcgccg acgcaaggct ggcggccgtc gccagggagt tcccaggcgc
     3001 agaggtggcg acgatggacg gctcggcagg gacgcacggg accgtgctcg acgccacaga
     3061 ctccctcgaa gggtatggag cggtctacgc ctgcgggccc aaccctatgt tggccgcggt
     3121 caagcgcgcg gcaagggaga acaggctatg ccagctctcc gtagaggaga ggatggggtg
     3181 cgggaacggc tcgtgcaacg gttgcgtggt ccccgtgcga agaggcggct acatcaagtc
     3241 ctgcatcgag ggacccgtct tccattcgga tgttctggcg tggtagtgcg cgagcagaag
     3301 gaaggtgtcg atctcgcgat cgagctctgt gggctcccgc tgcggacccc cctgatgccg
     3361 gcttccggaa cactctccaa agaagcccta ggcgaggtcg aaggcgtgta cggggcgatg
     3421 ctgccgaaga cgatcacccc ggctacaagg gccggcaatc caccgccccg catcgccgag
     3481 accccggcag ggatggtcaa ctcaatcggt ctccagaacc cgggtgtaca cggcttcttg
     3541 cagggtctgg acgcgttcga cctcggtata cctatcctcg tctccgtggc cggcgatacc
     3601 gtcgaggagt tcgcccttct ctgcgagatc ctcgccggag acgatcgcgt ctccgccgtc
     3661 gaactgaacc tctcgtgccc gaacgtggag tgtgggggcc tggccttctg cgcaggaccc
     3721 gcctccgtag aagaagtcgt cgccgcctgc cgcgccgccg tgcctggtaa acccatcatg
     3781 gcgaaactca ccaacgaagg tgtcgtgaag aacgcccgcg ccgccgagtc ggcaggggcg
     3841 gacgggctta cggtcataaa caccatcccc gcgctcaccg tagacgtccg cgcccgcaag
     3901 gttctggtgc ggggcggcct ctcgggcccc gcgataaagc ccgtagcact ccgggccgta
     3961 tacgacgtat caggtgtggt gaacgtaccc gtgataggta gcgggggcgt ggtgagcggg
     4021 acggatgtgg cggagttcat gctcgccggc gcgacggcgg ttcaggttgg cacagcaagc
     4081 ttcgtcaggg gcccgcgcga aatcctcgac gaattttcag ggtatctgaa agaaaacggc
     4141 ctgagcgctg ccgagctgac aggagcctta cgtcacccgt cttaggctgg atgccgccct
     4201 ctatgagcct gcctcagctc gccgtcctgg attgagagat cctggcttat cgcgctcatg
     4261 gttgcccttt cctaattttc ccagttctaa cccctcacag agcgtagccc cttcgcaaga
     4321 tgtggcgcac tcccacatgg gtgataggtg ctgttgccag tacgttgcct gcgggccgcg
     4381 aacttcggag aacgcctctt tccacgcatt acggtgaaaa cacagtacat aagaccgccc
     4441 tcggaaaggt ctatgcggag tgtgcgtata ataaagagtt aggcggcaac tcgagatgac
     4501 ggaggcgacg ccatggtgaa ctggctcaag tcactgttcg ctggatccaa gccggcgggg
     4561 ccgccgcagg tcctgcgagc cttcgcctcc gaccagccca cgatcaccca gaccggcatc
     4621 cgggtcgaaa acggcgcgtg gcacattgac actcgcgctc agcagacgat acgactcttt
     4681 gaggtggaaa accccgccgt ggagcattgc ctgctgacct atcgagcgga actcaaggca
     4741 gaaggcctcc aaggccgtgc ctttctcgaa atgtggtgcc gcctgccagg tcgcggcgaa
     4801 tacttctcga aaggctatca gcaggctgtg tcgggtacgg tcgactgggc tcgctacgag
     4861 acccccttct acctcaagag ggatcagaaa ccggatctca tcaagctcaa cgttgtggtt
     4921 gagggcccgg gcacagtgtg gctccgcaac gtggagctgc tcaagacacc gctcgcatcg
     4981 tgagggagga ggccgcggtt gccgccgaat aagcacatgc agctgagggg cgctcctaat
     5041 acaggccaga tcccgagcgc tggaggtaca gtctgcgccc tccatacgat cttccctcgc
     5101 actcgctgaa cgaattgccc agcttgcggg atagcgtctt atcgggccgc tcggggtgtc
     5161 caactcgtcg gacagcgcat ccggcagctc ggcttcccat cgtggaggtc ggcgcggtcg
     5221 tgccgtaagc ctgtcgccag gcttccagga tcaccaccca gtctcgggtg ccatcggacc
     5281 gctcggacag gtaggtcagg aagccctcca cactcactac ctcgagcacg ccgcatgtgg
     5341 ccggacggag ctccgaagag ttgccgctcg tagtccgcct tggggagagt tcgaagcccg
     5401 agctcgtgag cgagatggtg gggtaacaaa cctcgccgcg atgtctgcga cccatagcaa
     5461 gaccagcgtt gtcggcataa tggtcgccgt cgcagcacgc tctgggacgc gtgacgtcaa
     5521 agcaaggctg cccgccgacg ccggatcggg gagacgcttt tcgcgatgcg agacaccctc
     5581 ttctgcgtaa aattacccta gcaggaatca acgcaacccg gcagccgcgg aggccgacgt
     5641 ccgcgagaca ggaggtggcc atgatcgaga ccagggtggg gctacaggcg gcgctcaacg
     5701 gcgacaggac gaaggccgcc catcccgcca cgccggtgtc cgtcgaggag ctggcgcggc
     5761 acgccgccgc ctgcgtcgcc gcgagatcca cctcactccg g
//
LOCUS       DATMJA010000210         2867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_857150, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000210 DATMJA010000000
VERSION     DATMJA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857150"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /locus_tag="VNT12_04940"
     CDS             <1..1827
                     /locus_tag="VNT12_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWI45500.1"
                     /translation="KNRILKRGALPARTAAAVALQMAEALKAAHERDVIHRDIKPHNI
                     LITGLGDVKVTDFGIARAAASSTMTRTGHILGTAHYISPEQAMGEPVGPASDLYSLGV
                     VLYEMLTGELPFDADTPLGIAMKHLNGHLRPPKELNPSVPDGINAITLRLLAKNPEDR
                     YASDSELIEDLERVCAGLDPSATTTEMTPHALAATTRLNAPPPPQRVARNKKRRRRAE
                     PLLLALLALLVLAALAWAGYTLLQNWQAGNMAPQRVVPDLVGMSLDGAREKVGDDFEV
                     EVAKRAPGERPVDTILGQSPSTGKAEKGSTISVNVIGTRVSDVPDVVGSGRAEAEQAL
                     KAAGFEVEVDGRESSFDNQGQVMGQDPSGGAQAEVGSGVTVTVGTGPSSVPVPRLYGN
                     TPAQAESILQRAGLSLGTQSRDYNNDVTEGSIFFQDPSGGENVEPGTAVNVTVSLGLE
                     QVEVPEVYGLRVAAAQTALSNAGLNSTPLEVEGDEPAGTALATEPGAGAMLDPGSTVS
                     LYYSAGPPPATTASPDASQSPNSKPSQGPDRGPQDEGNTDAKPADQRQADRPQRNDRT
                     QDSSGAGNSGQGSSGQGSSGQGSSGQGSSGSGNGVGLGTNER"
     gene            complement(1876..2139)
                     /locus_tag="VNT12_04945"
     CDS             complement(1876..2139)
                     /locus_tag="VNT12_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45501.1"
                     /translation="MENDQAQESVRIRANSPGEFPILVVELPSGGLRAVYFETGYDLG
                     RAKTVEEDWLLENAVGRHSFIEVDPPVETPAGSLGDYVRRELL"
     gene            2250..>2867
                     /locus_tag="VNT12_04950"
     CDS             2250..>2867
                     /locus_tag="VNT12_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45502.1"
                     /translation="MSDPIPKDGGTEAGASEGIADEPLLDRLPPEDLPEELLLRRLGR
                     LDTRRALAEICRNVPALEEILLPRGIQADALTRKSGGGAPLLRGIARRISHDAAAWGA
                     FRTAIQEQIPPETFEALEGFSPERLEELKQAHTTEGLLLAALAADEEPEEGVVEALVD
                     AWREEKGEAEKKAFKDAHVKDLEAELERLKGENERLSFASRAAKER"
BASE COUNT          587 a          923 c          941 g          416 t
ORIGIN      
        1 aagaaccgca tcctcaagcg cggcgcgctc cccgcccgca cggcggcggc cgtggcgctc
       61 cagatggccg aggccttgaa ggctgcgcac gagcgcgacg tgatccaccg agacatcaag
      121 ccccacaaca tcctgatcac aggtctcggg gacgtaaagg tcacggactt tggtatagca
      181 cgcgctgcgg cgtcttcgac catgacgagg acgggtcaca tcctgggtac ggcccattac
      241 atctcgcccg agcaggcgat gggcgagccg gtcggtccgg cgagcgacct ctactctttg
      301 ggcgtcgtcc tctacgagat gctcacaggc gaacttccct tcgacgctga cacaccttta
      361 gggatcgcga tgaagcacct caacggacat ctgcgtccgc cgaaggaact caacccttcg
      421 gtgcccgacg gtataaacgc cataacgtta agactgctcg ccaagaaccc cgaagaccgc
      481 tacgcgagcg actcggagct catagaagat ctcgagcgcg tctgcgccgg cctcgacccc
      541 tccgccacga cgacggagat gacaccccac gccctcgcgg cgaccacgcg cctcaacgcc
      601 ccacctcccc cgcaaagggt agctcgtaac aagaagcggc gcaggagggc tgagccactg
      661 ctgctcgccc tcctcgcgct cctggtcctc gccgcactcg cctgggccgg gtacaccctt
      721 cttcagaact ggcaggccgg caatatggcg ccgcagagag tcgtcccaga cctggtcggc
      781 atgagcctgg atggcgccag agagaaggta ggggacgatt tcgaggtcga ggtcgcaaag
      841 cgcgcccccg gcgagagacc cgtcgacacc atactcggcc agagccccag caccggaaag
      901 gcggagaaag gctcgacgat ctcggtcaat gtcatcggga cgcgggtgtc ggatgtgccc
      961 gatgtcgtgg gctcggggcg cgcggaggcg gagcaggcgc tgaaggccgc cggcttcgaa
     1021 gtcgaggtcg acgggcggga gagctccttc gataaccagg gccaggtcat gggccaggac
     1081 ccatcgggcg gtgcgcaggc ggaggtggga tcaggggtga ccgtcaccgt cggtaccggc
     1141 ccttcgagcg tgcctgtccc gcggctgtac ggaaataccc cggcgcaggc ggagtctata
     1201 ctccagaggg cgggcctgag cttgggcacc cagagccggg actacaacaa tgacgttaca
     1261 gagggcagta tcttctttca agacccctca ggaggggaga acgtggagcc cggaaccgcg
     1321 gtcaacgtca ccgtcagcct gggtctggag caggtcgagg tccctgaagt ctacggcctg
     1381 cgcgtcgcgg cggcgcagac ggctctctcc aacgccgggc tgaactccac ccccctcgag
     1441 gtcgagggtg acgagccggc aggcaccgcg ctcgcgacgg aaccaggggc cggcgcgatg
     1501 ctggatccag gctccaccgt tagcctctac tacagcgcag gtccccctcc cgctacaacg
     1561 gcgtcccctg atgcctcaca gtcgcccaac tccaaaccat cgcaaggccc cgatcggggg
     1621 ccccaggacg aaggtaacac cgacgcgaaa cccgccgacc aacgacaggc cgatagaccg
     1681 caacgcaacg atcgcactca ggatagcagc ggagcgggta acagtggtca aggcagtagt
     1741 ggtcaaggca gtagtggtca gggcagtagt ggtcagggca gcagtggatc tggcaacggc
     1801 gtagggttgg gcacaaacga gcgctagggg tgaggaagcg aagagtaggg gcgctccgcg
     1861 gagcgcccct actctctaga ggagctcccg cctcacgtag tctccaaggg accctgcggg
     1921 cgtctccacg ggaggatcta cttcaatgaa gctgtggcgg ccgacagcgt tctccaggag
     1981 ccagtcttcc tcgacggtct tggcacgccc gaggtcatag ccggtctcga agtagaccgc
     2041 ccgtagcccg cccgaaggaa gctcgaccac gaggatgggg aattctccgg gggagttcgc
     2101 ccggatcctg acgctctctt gtgcctggtc gttttccatg cctggagtct atacgagatc
     2161 cgaagccggc ggggactcag aagggccgtg ataaaatcac gggagcatga acagaccata
     2221 tgacattcat attcgtggag ggagtcagca tgtcagatcc catacccaaa gacggaggaa
     2281 ctgaggcagg agcctcagag ggaatcgccg atgaacccct gctcgacagg ctgccgcccg
     2341 aggatctacc cgaggagttg ctcctgcggc gtctcgggag gctcgacacc aggcgcgcgc
     2401 tcgccgagat ctgcaggaac gtgcctgccc tcgaggagat ccttcttcca aggggcatac
     2461 aggccgacgc tctgacgcgc aagtcaggcg gtggcgcgcc gctcctgcgg ggcatcgcac
     2521 gcaggatctc ccacgacgcg gctgcctggg gagcgttccg cacagccatc caggagcaga
     2581 tcccacccga gaccttcgaa gctctggaag gtttctcccc ggagagactg gaagaactca
     2641 agcaggccca caccaccgag ggcctgctgc tggccgcact cgccgccgac gaggagcccg
     2701 aagagggcgt cgtggaggcg ctcgtggacg cctggcgcga agagaagggc gaggccgaga
     2761 aaaaggcctt taaagacgcg cacgtcaagg acctggaggc cgagctggag cgcttgaaag
     2821 gcgagaacga gcggctctct ttcgcgtcca gggccgccaa ggagagg
//
LOCUS       DATMJA010000211         4101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_561633, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000211 DATMJA010000000
VERSION     DATMJA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561633"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /gene="metG"
                     /locus_tag="VNT12_04955"
     CDS             complement(<1..1231)
                     /gene="metG"
                     /locus_tag="VNT12_04955"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWI45503.1"
                     /translation="MSERRRRYLVTSALPYANGPLHVGQISGAYLPADAFVRYLRARG
                     EDVVYACGTDEHGVPITLTAEREGKDPQEVVDYWWANMKETFERFGISFDNFSRTSTP
                     LHAKNTQLFYRKLKEGGYISEAESKQMYSPTEGRFLPDRYVEGTCPVCGYKEARGDQC
                     DNCGSWYEAYELIEPHSKVTGGPVEVRETTHLYLDLPEFSDRLKEYVAGQEHWRTSVK
                     NFILGIIDGGLEKRPITRDIQWGVPVPEPGFEDKRFYVWFDACIGYVSSTMEWAQNIG
                     EPDRWREYWQEPESRLIHFIGKDNTVFHTLIWPAQLMGTAEDGEEPFVLPYDVPANEF
                     MNLEVLVDGERRAMQMSTSRNLAVWLHEALDRFPADPLRFYLASNLPETGDVVFSWRE
                     FQSRVNSDLIGNLANYIN"
     gene            1385..1840
                     /gene="dut"
                     /locus_tag="VNT12_04960"
     CDS             1385..1840
                     /gene="dut"
                     /locus_tag="VNT12_04960"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWI45504.1"
                     /translation="MSSERPLQVPFRRLVPEAGMPERAHLGDAGYDLRSVERVGLAPG
                     ARALVRTGISVAIPEGYAGLVLPRSGLAMRYGVSLVNTPGLIDSGYRGEIQVPLINHD
                     REETFLIEEGTRIAQLVLVRAASALFAEVEFLESAADGRGEGGFGSSGF"
     gene            1785..2306
                     /locus_tag="VNT12_04965"
     CDS             1785..2306
                     /locus_tag="VNT12_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HWI45505.1"
                     /translation="MSRQPTVVGKAASAPPGSSVRFLCDAMLGGLAKWLRAAGYDAYY
                     AREGTDVSDRTLTAKALRERRVLLTSDGGFLERKPVRDGSVGFLTVPHLPLEDQLRLV
                     VEHFGLVRRQSRCMVCSGRLETVPREAVAGRVPPGVLRDHERFFLCQDCGRIFWHGSH
                     WQRISDRLDRVFV"
     gene            2411..2980
                     /locus_tag="VNT12_04970"
     CDS             2411..2980
                     /locus_tag="VNT12_04970"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWI45506.1"
                     /translation="MNATTHAVFGIAALAGAALVTGADPPAYLYPAAVAAAWLPDIDN
                     PRSTLGNGLNRLKNPTLNLLSRPLSWALKTASFVLVRTVGHRTMTHSLAGVLLFSLLV
                     MLFLGRFPNLVLALVAGYASHVFADALNTRGVPLLWPMGKPFRLLPGGVRSGGAVEVA
                     VALVALAFTLYALLLLYPGLRGAIGLQPI"
     gene            complement(2930..3493)
                     /gene="yqeK"
                     /locus_tag="VNT12_04975"
     CDS             complement(2930..3493)
                     /gene="yqeK"
                     /locus_tag="VNT12_04975"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HWI45507.1"
                     /translation="MARARLSERRYEHTLRVADTAENLALAHHLDADRARLAALVHDA
                     AREMGPEEFLYLADKWHLQVGDPERQSPKLLHGPVAAELARRELGVDDEEVLEAVRTH
                     TTGRAEMRPLALVLYVADKIEPARDYPSVGRLRRLALEDLNGAAAESLRRAIAHNEER
                     GKATHPASLKMLDWLEADRTAQSRIEK"
     gene            3565..>4101
                     /locus_tag="VNT12_04980"
     CDS             3565..>4101
                     /locus_tag="VNT12_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HWI45508.1"
                     /translation="MRDGRNIERAEIVTVGTEMLLGDLVDTNTAWISQRLAELGVGIY
                     RHTTVGDNSERIVAALREAASGSDLVVTTGGLGPTSDDLTNASLSTLTGRAMVEYPEA
                     REHVDRMFTKFGRKPTANNYKQALFPKGTELIPNPVGTAMGALVEWEGTLFATLPGVP
                     TEMKSMFEATLEPLIRARS"
BASE COUNT          684 a         1350 c         1282 g          785 t
ORIGIN      
        1 ggtttatgta gttggcgagg ttcccgatca ggtccgagtt caccctgctc tggaactcgc
       61 gccaggagaa caccacgtcg cccgtctcgg gcaggttcga ggccagatag aaacgcagag
      121 ggtcggcagg gaagcggtcg agggcctcgt gcaaccagac cgcgaggttg cggctcgtgg
      181 acatctgcat cgcccgccgc tccccgtcga ccaggacctc caggttcatg aactcgttgg
      241 caggtacgtc gtagggtagg acgaacggct cctcaccgtc ttctgccgtg cccattagct
      301 gggccggcca gatcagggtg tggaagaccg tgttgtcctt accgataaag tggatgagcc
      361 ggctctcggg ctcctgccag tactccctcc accggtcggg ctctccgatg ttctgggccc
      421 actccatcgt cgaggagacg tagccgatgc aggcgtcgaa ccacacgtag aagcgcttgt
      481 cctcgaagcc aggctcgggg acagggacgc cccactggat gtcccgcgtt atgggccgct
      541 tctccaggcc gccgtctatg atgccgagga tgaagttctt gacggacgtg cgccagtgct
      601 cctgtcccgc cacgtactcc ttcagcctgt cggagaactc ggggaggtcg aggtagaggt
      661 gcgtagtctc cctgacctcg acaggcccgc ccgtgacctt ggagtgcggc tcgatcagct
      721 cgtacgcctc gtaccacgag ccgcagttgt cgcactggtc gcccctcgcc tctttgtagc
      781 cgcacacggg gcaggtcccc tctacgtatc ggtcgggcag aaagcggccc tccgttgggc
      841 tatacatctg cttggactcg gcctccgaga tgtagccacc ctccttgagc ttgcggtaga
      901 agagctgggt attcttcgca tgaagcgggg tcgaggtgcg cgagaagtta tcgaaggaga
      961 tgccgaaacg ttcgaacgtc tccttcatgt tggcccacca gtagtcgacg acctcctgcg
     1021 gatccttccc ctcacgctcc gccgtcaacg tgatcgggac cccgtgctcg tcggtgccgc
     1081 aggcgtagac cacgtcctcg ccacgcgccc gcagataacg cacgaacgcg tcggcaggaa
     1141 ggtaagcgcc cgagatctgc cctacatgca aaggcccgtt tgcataaggc aacgccgagg
     1201 ttacgagata tcgtcgtctc cgctcgctca tgagctaagg ataacagcag aaggctatag
     1261 acatcaggta tcagccgtca ggtaggggca ttgccttgat gttagtcttg cccggtggcg
     1321 taccacctcc atcctgaggc ccgaaccctg tagcctgaaa ctttcgagtg ctactattct
     1381 ccggatgagc tccgaacgcc ctctgcaggt cccttttcgc cgtctcgtcc ccgaggcggg
     1441 gatgcccgag cgcgcccacc tgggggatgc gggctacgac ctacgttcgg tcgagagggt
     1501 gggcctggca ccgggagccc gggcgctggt gcggacaggg atctctgtag ctatacctga
     1561 gggttatgcg gggctcgtgc tcccccgcag cggcctcgct atgaggtatg gggtctccct
     1621 cgtcaatacg cctggcctca tagactccgg ctaccgcggt gagatccagg tccccctaat
     1681 aaaccatgac cgcgaggaga ccttcctgat cgaggaggga acgaggatcg cgcagctcgt
     1741 cctcgtacga gccgcctcag cactcttcgc cgaggtagag ttccttgagt cggcagccga
     1801 cggtcgtggg gaaggcggct tcggctcctc cgggttctag cgtgaggttc ctctgcgacg
     1861 cgatgctcgg cgggctggcg aagtggctgc gcgccgccgg gtacgacgcc tactacgcca
     1921 gggaggggac ggacgtctcg gacaggaccc tcaccgcgaa agccctccgg gagaggcggg
     1981 tgctcctgac cagcgacggg ggtttcctgg aacgcaagcc tgtacgtgac gggagcgtgg
     2041 gcttcctaac ggtgccgcac cttccgctcg aagaccagct ccggctcgtg gtcgaacact
     2101 tcgggctcgt caggcgccag agccgctgca tggtgtgcag cgggagattg gagaccgtac
     2161 cccgcgaggc cgtcgcgggc cgagttccgc ccggcgtgct acgggatcac gaacgcttct
     2221 ttctctgcca ggactgcgga cgcatcttct ggcacggctc gcactggcag cgtattagcg
     2281 accgcctcga tagagtgttc gtttgaggga gaagcccggc aagtacgcgg agcgccggct
     2341 tgttcctgtt agacgagcct tccctgcagc ggatcgcccg gttccgtcga gttgctaaac
     2401 tgcatgagga ttgaacgcca ccacccacgc cgtcttcggg atcgcggctc tggccggcgc
     2461 cgcgctcgtc acgggggccg acccgcccgc ctacctctac ccggccgccg tcgccgccgc
     2521 ctggctgccc gacatcgaca acccgcgcag caccctgggc aacggcctga accggctgaa
     2581 aaatcctacg ctcaacctcc tgagccggcc cttgagctgg gccctgaaga cggcgtcttt
     2641 cgtgctagtc agaactgtcg gccaccgcac catgacccat tcgcttgccg gtgttctcct
     2701 cttttctctg ctggtcatgc tattcctggg acgttttccg aacctcgtcc tcgccctcgt
     2761 tgccggctac gcctcgcacg tcttcgccga cgcgctcaac acccgcggcg tgccgctttt
     2821 atggcccatg ggcaagccgt tcaggctttt gcccggaggt gtcaggtcag gaggtgccgt
     2881 ggaggttgcc gtggctctcg tagcccttgc cttcacgctc tacgccttgc tacttctcta
     2941 tcctggattg cgcggtgcga tcggcctcca gccaatctag catcttgagg ctcgcagggt
     3001 gggtggcctt ccctcgctct tcgttgtggg caatggcgcg ccgcaacgac tccgcggccg
     3061 ccccgttgag gtcctccaag gctagcctgc gcagacgtcc cacgctcggg tagtcgcggg
     3121 ctggttcgat cttgtccgcg acgtagagta cgagcgcgag cggcctcatc tccgctctcc
     3181 ccgtcgtgtg ggtcctgacc gcctcgagaa cctcctcgtc atcgacgcca agctcccgcc
     3241 ttgccagctc ggctgccacg ggcccgtgca gtagcttcgg gctctgccgc tccggatctc
     3301 ccacctgaag atgccattta tcagccaggt aaaggaactc ttcgggcccc atctcgcggg
     3361 ctgcatcgtg caccagggcg gccagccgtg cccgatccgc gtcgagatgg tgggccagcg
     3421 ccaagttctc ggccgtgtca gccaccctca gggtgtgttc gtaacgcctc tccgagagcc
     3481 gggcacgggc caaggcttcg gctctttcga ggagagtgtt ggttttcgcg tggtcctcca
     3541 tccggcggat tgtagaatac ctgcgtgcgt gatgggagaa acatagagcg cgcggagatc
     3601 gtgactgtag gtaccgagat gctcctgggc gacctcgtgg atacgaacac ggcctggatc
     3661 agccagcgcc tcgccgaact cggggtgggc atctaccgcc acaccaccgt gggggacaac
     3721 tcggagagga tcgtcgccgc cctgcgcgaa gccgcctccg gctccgacct tgtggtaacc
     3781 acaggcggtc tcggcccaac ctcggacgac ctgaccaacg ccagcctctc gaccctgaca
     3841 ggtcgcgcga tggtcgagta tcccgaggct agggaacacg tggacaggat gttcacaaag
     3901 ttcggccgca agccgacggc caacaactac aagcaggctc tcttccccaa gggaaccgaa
     3961 ctcatcccca acccggtggg gacggcgatg ggtgcgctcg tggagtggga agggacgctg
     4021 ttcgccacac ttccaggagt gccaacggag atgaagagca tgttcgaagc gacgctcgaa
     4081 cccctgatcc gggcccgcag c
//
LOCUS       DATMJA010000212         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_799106, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000212 DATMJA010000000
VERSION     DATMJA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799106"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VNT12_04985"
     CDS             <1..270
                     /locus_tag="VNT12_04985"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564627.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWI45509.1"
                     /translation="KDTGVTGKELEDSLEAVGITCNKNMVPNDPAPPTVTSGIRLGTA
                     AMTTRGMGEDEMQEIADIIVATTHGKTDGLRDRVKILTEAYPLYE"
     gene            263..910
                     /gene="upp"
                     /locus_tag="VNT12_04990"
     CDS             263..910
                     /gene="upp"
                     /locus_tag="VNT12_04990"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HWI45510.1"
                     /translation="MSETTPDVKNLYVVDGPLSRHKLAVLRDERTTTAGFRQAMKELA
                     LILVAEATGNMPTSTVQIRTPLIETEVEEISGPVCLVPVLRAGLGMLDGALALLPTAT
                     VGFMGLQRDEETAQPIEYYVNLPRNLDEYLVLVLDPMLATGGSLSATIDKLKEQGATW
                     ISCLHAVAAVPGVERVSSEHPDVTFYTAAVDPELDVRSFIVPGLGDAGDRLYGTL"
     gene            897..1385
                     /locus_tag="VNT12_04995"
     CDS             897..1385
                     /locus_tag="VNT12_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HWI45511.1"
                     /translation="MEHFEDPAAGTSNSHETLKKVRPSWDEYFMRIAHEVATRSTCPR
                     LAVGAVIVRDKRILTTGYNGSPSGMPHCEDVGCLIRIVDGRESCQRTLHAEQNAIIQA
                     AYHGVSVTGASIYCTHQPCLMCVKMIMNAGIDEVHYAGGYPDPLAMELATEGGLAMIR
                     FA"
     gene            complement(1448..2104)
                     /locus_tag="VNT12_05000"
     CDS             complement(1448..2104)
                     /locus_tag="VNT12_05000"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI45512.1"
                     /translation="MSGDRGPRRRRRPEEAERAILAAARAFLEERPFREMTVEGVMVR
                     TGLSRPAFYAYFRDRYDLVTRLLEGVGSLLFALDWRWLSGGEGGDEAREVLVDALRAG
                     SRTFVEYGPVLRAIADAAGYDARVEQVYRYGLIERLVGAVATRISRDVEAGLSPSDFD
                     PQETARALVLMTERYLLDAFGRPERRPSRRESAAVFGTLEKIWVRTLYGRGSRRGGSR
                     "
     gene            2378..2602
                     /locus_tag="VNT12_05005"
     CDS             2378..2602
                     /locus_tag="VNT12_05005"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWI45513.1"
                     /translation="MPDTRSQRNDASGNYARFAGLGISLLLIFSLPTVVGYFLDRLAG
                     TLPLFLLIGLSLGFVVGMFYVYRALQKLGG"
     gene            2620..3030
                     /locus_tag="VNT12_05010"
     CDS             2620..3030
                     /locus_tag="VNT12_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45514.1"
                     /translation="MAFRCAGWIFALMIPVGALLLYVYGVAHAEAFAYGVGIGLVSLL
                     STALTVSLLWGPNMAVGVMVGVFSFVGRCILAAGALGVPAYLGLWPVLPMLGGFAGVY
                     LAENIVLMPKALKISHASIRREATRKSVERRTEA"
     gene            3027..3812
                     /gene="atpB"
                     /locus_tag="VNT12_05015"
     CDS             3027..3812
                     /gene="atpB"
                     /locus_tag="VNT12_05015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWI45515.1"
                     /translation="MTGALAQISQDELREKILHTFEAARHAWRIPLHIGPVDISINKS
                     IWFLWFGAAVTFLILFVGSRLLRDRPGAYQVIVEEVYGFGRNLGGQVGEEGRKWFPYT
                     LSLFVFILVLNLIGLIPNTYPVTANISFTLILALLTFILTQYEGFRRNGAGTYLKSFA
                     PPGLPLKPLMVPFMWGIEVLGEFTKPLTLGMRLYANILAGHLIIYVFLGLILYFGTVV
                     AIISVPLSVVFYAFEVFVAILQAYIFAILTQVYIELAMFREDH"
BASE COUNT          758 a         1229 c         1145 g          761 t
ORIGIN      
        1 aaagataccg gcgtcacggg caaggaacta gaagattcgc tggaggccgt cggcatcacc
       61 tgcaataaga acatggtccc gaacgacccc gcacccccga ccgtgaccag cggcatcagg
      121 ctcggcaccg cggcgatgac gacccgcggc atgggtgagg acgagatgca ggagatagca
      181 gacatcatcg tcgcaaccac gcacggaaag acagacggcc tgcgcgacag ggtgaagatc
      241 cttacggagg cttacccgct gtatgagtga gaccactccc gatgtgaaga acctctacgt
      301 cgtagacggg cctctgagcc gccacaagct tgcggtcctg cgcgacgagc ggaccacgac
      361 cgccggcttc aggcaggcca tgaaggagct cgcccttatc ttggtcgccg aggccacagg
      421 aaacatgcct accagtacgg tccagattcg tacgcccttg atcgagactg aggtcgagga
      481 gatctccggt cccgtctgcc tcgttcctgt cctccgcgcc ggtctcggta tgctcgacgg
      541 ggcgcttgcc ctcctgccga cggcgacggt cggctttatg ggcctccagc gcgacgagga
      601 gacggcccag cctatcgaat actacgtcaa cctcccacgc aacctggacg agtatctagt
      661 cctcgtcctc gatccgatgc tcgcgactgg ggggagcctc tcggccacga ttgacaagct
      721 caaggaacag ggggcgacct ggatctcctg cctccacgcg gtcgccgcgg tgccaggcgt
      781 agagcgcgtt tccagtgagc accccgacgt gaccttctac accgcagccg tcgacccgga
      841 actggatgtt cgctcgttca tcgtccccgg cctcggcgac gctggagaca ggctctatgg
      901 aacactttga agaccccgca gcaggcacct cgaactccca tgagaccctg aagaaagtcc
      961 gtccttcatg ggatgagtac ttcatgcgca tagcgcacga ggtcgccacc cgctcgacat
     1021 gcccgcgtct cgccgtaggg gctgtgatcg tgcgcgacaa acgtatcctg accaccggct
     1081 acaacggcag cccttcaggg atgccccact gcgaggacgt gggatgcctc attaggatcg
     1141 tggacggccg cgagagttgc cagcgtacgc tccacgcgga gcagaacgcc atcatccagg
     1201 ccgcctacca cggtgtctcc gtcacaggcg cctcgatcta ctgcacccac cagccatgcc
     1261 tcatgtgcgt gaagatgatc atgaacgccg gtatcgacga agtgcactac gcaggcggct
     1321 atcctgatcc cctggcaatg gaactcgcca ccgaaggcgg cctcgccatg atccggttcg
     1381 cgtaaggccg tacccgcaca tggaattccg cccgcaaatg gccgtagggg cggttcgcga
     1441 accgccgtca ccgcgaaccg ccccgtcgac tcccccgacc atacagcgtc ctgacccaga
     1501 tcttctccag ggtcccgaag accgccgcgc tctcccggcg cgatggccgg cgttcgggac
     1561 gcccgaaggc gtcaagcagg taacgttcgg tcatgagcac cagcgcccgg gccgtctcct
     1621 gcgggtcgaa gtcggaaggg gacagaccag cctccacgtc gcgtgagatg cgggtcgcca
     1681 ccgcaccgac gaggcgttcg atcagaccgt acctgtaaac ctgctcgacc ctggcatcgt
     1741 agcccgccgc atcggcgata gcccgcaaca cgggcccgta ctcgacgaag gtccgcgaac
     1801 ccgcccgcaa cgcgtcgacc agaacctccc tcgcttcgtc cccgccctcg ccaccagata
     1861 gccagcgcca gtcaagcgcg aagagtaggc tgccaacacc ctcgagcaaa cgcgtaacaa
     1921 ggtcgtaacg gtcccgaaag tacgcgtaga aagccggtcg cgacagcccg gtcctgacca
     1981 taaccccctc cacggtcatc tccctgaaag ggcgctcctc cagaaacgcc cgcgccgcag
     2041 caagtatcgc ccgctcggcc tcctcaggcc tgcgtcgcct gcgcggaccc cggtccccgc
     2101 tcacctcatc accactctct agccaactct ctcctccaat gagagacaat gtaacgcttt
     2161 attgacaccg tgtctattga cgtcatgtca ataacgagat atcatcctgg tagagagcca
     2221 cgagaggaga gatccgtgga cggtagcgag gcgagggagg ttcggctgga gcggcgagag
     2281 cccgcctcgg gcgtcctgcg gaggttcgca ggaaagggca ttttccggag attgacactg
     2341 attgcaacag attagcccgt aaggtatagt tccggtgatg cccgacacca gatctcaaag
     2401 gaacgacgcg agcggcaact acgcacgttt cgcaggtctt ggaatttcgc tcctcttgat
     2461 cttctctctc cccaccgtgg tgggttactt tttggacagg ctggccggta cgcttccttt
     2521 gtttctactt atcgggcttt cgttagggtt tgtcgtgggg atgttctacg tgtacagggc
     2581 gctccagaag ttgggcggct aatgggcgat cactggcggt tggctttccg atgcgccggc
     2641 tggatcttcg ctttgatgat ccctgttggg gcgttgcttc tgtatgtcta cggcgtcgcc
     2701 catgccgaag ccttcgctta cggagtaggg ataggacttg tgagcctgct ctctacggcg
     2761 cttacggtct ctttgttgtg gggaccgaac atggcggttg gtgtgatggt aggggtcttc
     2821 tccttcgtgg gtcggtgcat tctcgcggca ggagcgcttg gcgttcccgc ctatctaggg
     2881 ctctggcccg tgttgccgat gctgggaggc ttcgcagggg tctatctagc cgagaacatc
     2941 gtgctcatgc ctaaagcgtt gaagatcagc catgcgagca taaggcgtga ggcgacccgc
     3001 aagagcgtag aacggaggac ggaggcttga ccggtgctct ggcgcagata agccaggacg
     3061 agctgcgaga gaagatcctg cacacgttcg aggccgccag gcacgcctgg cgtataccgc
     3121 tgcacatagg gccggtagac atctcgatta acaagagcat ctggttcttg tggttcgggg
     3181 cggccgtgac ctttttgatc ctgttcgtgg gcagccggtt gctcagggac aggccaggcg
     3241 cctaccaggt gatcgtcgag gaggtctacg gtttcgggcg aaacctcggc gggcaggtcg
     3301 gggaggaggg aaggaagtgg ttcccttaca cgctctccct cttcgtgttc atcctggtgc
     3361 tcaacctgat cggcctaatc cccaatacct accccgtcac cgcgaacatc tcatttaccc
     3421 tgattctcgc gctgctcacg ttcatcctca cccagtacga gggcttcagg cgcaatggcg
     3481 cgggaaccta tctcaagagc ttcgcgcctc cagggttgcc gctcaagccg ctcatggtgc
     3541 ctttcatgtg gggaatcgaa gttctcggcg agttcaccaa gcctctcacg ctcgggatgc
     3601 ggctgtatgc caacatcctg gctgggcacc tgatcatcta cgtcttcctc gggctcatcc
     3661 tgtacttcgg taccgtcgtc gcgataatct ccgtaccact gtccgtagtg ttctatgcat
     3721 tcgaggtctt tgtggccatc ttgcaggctt acatattcgc tatactcacc caggtttaca
     3781 tcgagttggc catgttccgc gaggaccatt aacggttaaa ggtaactcaa gagagtcagc
     3841 cgagaggcga aaggagtaag aagatggaca cattggcgat gttgctaccc atg
//
LOCUS       DATMJA010000213         6877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_324962, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000213 DATMJA010000000
VERSION     DATMJA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324962"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VNT12_05020"
     CDS             <1..1036
                     /locus_tag="VNT12_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWI45516.1"
                     /translation="SRTINAYARQKGLLKEKEESLTGDDIREGLAAVISVKLQEPQFE
                     GQTKTKLGNTEVKGLVESVTNRYLAEFLEEHPGEAKAIVNKALQAARARLAARTVREK
                     IRKGYLESSTLPGKLADCSSKDPARSELYIVEGNSAGGSAKGGRDRNFQAILPLRGKI
                     LNVEKANINKILSNAEIQALISAIGAGVGESFDLEQARYNKIVIMADADVDGSHIRTL
                     VLTFLYRNMRDLIEAGYVYIALPPLYKITHNRREHYAKNDVELRETMDRIRANGKASI
                     SRFKGLSEMNPQQLWETTMDPQNRTMLQVTVESAAVADELFTALMGDKVEPRKQFIEE
                     NAREVKNLDA"
     gene            1029..3593
                     /gene="gyrA"
                     /locus_tag="VNT12_05025"
     CDS             1029..3593
                     /gene="gyrA"
                     /locus_tag="VNT12_05025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWI45517.1"
                     /translation="MLDTFSGNVQPHSIEDELRDSFLSYAMSVIVSRALPDARDGLKP
                     VHRRVLYAMQDLGLQPNRPYRKSATVVGDVMGKYHPHGDQAIYDALARMAQDFALRYP
                     LVDGQGNFGSVDGDPPGAMRYTEARLTRMATEMLRDINADTVDFVPNFDESLKQPEVL
                     PARFPNLLVNGSDGIAVGMATKIPPHNLGEVVDATIALIDDPDLDDEEIAEHIKGPDF
                     PTGGVVVGLRGIRDAIQTGRGSVRVQAKAHTEQIKGNRTQIVVTEIPYQVNKSYLLQK
                     IAELVKERKLQDISDLRDESDRNGMRIVVELKREAVPKVVLNNLYKHTQMQQSFGVNL
                     VALVDGVPRTLSYGQALGHYIAHQFEVVTRRTRYELGRAKARAHILEGLLIALGDLDQ
                     VVATIRRSRSVETARKNLMKGFGLSERQAQAILDLRLQRLTAMERQKVQQEHRDLREK
                     IEYLEGILADESKVYGIVKEELGEVRAAYADDRRTAITAEEGVDFEIEDLIAEEEMVI
                     SISNGGYLKSVPVNSFRKQGRGGVGVAGMELKEGDYIDHLFITTTHHYMLFFTNKGKV
                     YRLKVHQLPRMGRAAKGRHIANMLPLAQGEHIASVLATKEFGEAEYLLFATKRGIVKK
                     TRFLEYNTPLKNDGIIAINLAGEDELISVRYASEGDEVVIVSRNGKGIRFKQADARPM
                     GRATAGVKGITLGKDDVVLSMDVISDDAADLFILTERGFGKRTALSQYRAQARGGQGV
                     IAMKTDGERGKLAGVRVVRPGLHELMIVSNFGTTIRIDADSVSRQGRAAQGVRVMNLR
                     SGDSVSAIAKVISSRSTHTDGATDELTLDELSGESQTGTSTAGLPEADGNGAVPGA"
     gene            3586..3999
                     /locus_tag="VNT12_05030"
     CDS             3586..3999
                     /locus_tag="VNT12_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45518.1"
                     /translation="MHSECEGVVECTALVLAESCTEYEDRLTLFGVLDEIRPNGASGT
                     SFVVYAKFEGCEHASDKERRARILVTDEDGEVLMESAEYSVDLDGTEKPETIVAAFDL
                     PEGFEVGEQLLWIEAVLDEETLSQTAVRIHDRYSV"
     gene            4056..5246
                     /locus_tag="VNT12_05035"
     CDS             4056..5246
                     /locus_tag="VNT12_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWI45519.1"
                     /translation="MSEDLAVFIDWENIYISTVTEYKSRPNVSAILEKAREYGRIVAA
                     TAYADWTDGDFRQAPPTLYSNGISPRYISARYFPGGKSQKRRTNSIDVMLAVECSDFL
                     HNHPQVDTYVLVTGDGDFIPLVSLLRSKGKNVVVIGVSEATSYHLIESADHFVSYASL
                     LKEDRAARVQDREPKKEAADPYEELVRAVQTLKKGGKTRVLGQVKQQMIVQLGSFDER
                     NAGFKKFKDFVVEAERRGLVNTVDQDLELQVYLPDEKVPDLQETDLAAEEPTSQSQSA
                     NRNRGNGRPAREERQQEEPEPGFDLLEDLTPYELKTVCTSVASLEQPATFVEIFSSIR
                     DLDERGELELSREEIGDVITAMLDANYLGKVRQRPYKQAKADMTFYALNAEKQEVKSA
                     LNSG"
     gene            complement(5380..5661)
                     /locus_tag="VNT12_05040"
     CDS             complement(5380..5661)
                     /locus_tag="VNT12_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45520.1"
                     /translation="METMLDLGVGILRLIPLILAFYIPALFGMATWSEGDRARAILWF
                     AIGFGAIVAVQILFSSVSAVQMVWVVALSLVQIAAALSLAALTVYKLAD"
     gene            5758..6132
                     /locus_tag="VNT12_05045"
     CDS             5758..6132
                     /locus_tag="VNT12_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45521.1"
                     /translation="MGINQRVSLRKFIDDIRRWVEEDRSDEWIASALGTSPSSVQSFR
                     SRNAIYRRGGGSTLHDPQEYSSYEGVLEPEGPGIWFDPEVGNDPRWQKRWKSTERVEL
                     RLTPTRMVLVRRGGARRSSLGS"
     gene            6150..6548
                     /gene="rnpB"
                     /locus_tag="VNT12_05050"
     ncRNA           6150..6548
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNT12_05050"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6657..>6877)
                     /locus_tag="VNT12_05055"
     CDS             complement(6657..>6877)
                     /locus_tag="VNT12_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45522.1"
                     /translation="LFELSRPLDELEGQLLHDFAGRTLTMRKIYEAHSVDRPFLAGHY
                     KEALIKLEAKGAIEADPPAQSRRKKHLR"
BASE COUNT         1510 a         2073 c         2171 g         1123 t
ORIGIN      
        1 gtcgcggacg atcaacgcct acgcccgtca gaagggcctc ctcaaggaga aggaggagag
       61 ccttaccggt gacgacatca gggaagggtt ggccgcggtc atctccgtca agttgcagga
      121 gccccaattc gaggggcaga ccaagaccaa gctcgggaac acggaggtca agggtctcgt
      181 cgagagcgtg accaaccgtt acctggccga gttcctggag gagcatcctg gtgaggccaa
      241 ggcgatcgtc aacaaggcgt tgcaggccgc gcgggcgcgg ctcgcggcca ggacggtgag
      301 ggagaagatc cgcaagggct acctggagag ctcgacgctg cctggcaagc tcgccgattg
      361 ctcatccaag gaccctgcta ggagcgagct gtacatcgtc gagggcaact cggccggcgg
      421 atcggcgaag ggcggaaggg acagaaactt ccaggccata ctgccgttgc gcggcaagat
      481 cctcaacgta gagaaagcca acatcaacaa gatcctctca aatgctgaaa tacaggccct
      541 gataagcgcg ataggcgccg gtgtggggga gagcttcgat ctcgagcagg cgcggtacaa
      601 caagatcgtc attatggccg acgccgacgt ggacggaagt catattcgaa cgttggtgct
      661 gacgtttttg tatcgcaaca tgcgagattt gatcgaggcc ggctacgtct acatcgctct
      721 tcctccgctc tacaagatca cacacaatcg ccgcgagcac tacgccaaaa acgacgtcga
      781 gttgagagag acgatggatc gtatcagggc caacggaaaa gccagtatct cccgcttcaa
      841 gggcctctcg gagatgaacc cccagcagct ctgggagact acgatggacc cgcagaacag
      901 gacgatgctc caggtcacgg tcgagtcggc ggcggtggcg gatgagctgt tcaccgcgct
      961 gatgggtgac aaggtagagc cgcgcaaaca gtttatcgag gagaatgcca gagaggtgaa
     1021 aaacctggat gcttgatact ttctcgggca acgtacagcc ccactccatt gaggatgagc
     1081 taagagactc tttcctttcc tacgcaatga gcgtgatcgt ctcgcgcgcc ttgcctgacg
     1141 cgcgcgacgg cctgaagccc gtccaccggc gagttctcta cgcgatgcag gatctcggcc
     1201 tgcagcccaa caggccttac cgtaagagcg cgacggtcgt cggagacgtc atgggcaaat
     1261 accatccgca cggggaccag gccatatacg acgcgctggc acgcatggcc caggacttcg
     1321 cgctcagata cccgctcgtg gacggccagg ggaacttcgg gagcgtggac ggagatccac
     1381 ctggcgcgat gaggtacaca gaagcccggc tcacccgcat ggcgaccgag atgctgaggg
     1441 acataaacgc cgacacggtt gacttcgttc cgaacttcga cgagagcctg aaacagcccg
     1501 aggtcttgcc ggcgcggttc cccaacctgc tcgtcaacgg gtctgacggc atcgcggtcg
     1561 gtatggccac taagatccca cctcacaacc tcggcgaggt cgtcgacgcg acgatagccc
     1621 tgatagacga cccggacctg gatgacgagg agatcgcaga gcacatcaag ggccccgatt
     1681 tcccgactgg gggtgttgtc gtcgggttgc gcggcatacg cgacgccatt cagactgggc
     1741 ggggctccgt acgggtgcag gccaaagccc acaccgagca gatcaagggc aacaggaccc
     1801 agatcgtggt cacggagatc ccttaccagg tcaacaagag ctacctcttg cagaagatcg
     1861 ccgagctggt caaggaacgc aaactgcagg acatctccga cctgcgcgac gagtcggacc
     1921 ggaacggcat gcgcatagtg gtcgaactga agcgcgaggc cgtgcccaag gtcgtcctca
     1981 acaacctcta caagcacacc cagatgcagc agagcttcgg ggtgaacctg gttgcgctcg
     2041 tcgacggcgt gccgaggacg ctctcctacg ggcaggccct ggggcactac atcgcccacc
     2101 agttcgaggt ggtgacgcgc aggacgcgct acgagctcgg gcgggccaag gcgcgggcgc
     2161 acatcctcga aggtctgctc atcgcgcttg gggacctcga ccaggtcgtg gctaccatcc
     2221 gccgctcgcg cagcgtggag acggcgcgca agaacctgat gaaggggttc gggctctccg
     2281 agcgccaggc gcaggccatc ctcgacctca ggttgcagag gctcaccgct atggagcgcc
     2341 agaaggtcca gcaggagcac agggacctgc gcgagaagat cgagtatctc gagggcatcc
     2401 tggccgatga gtccaaggtc tacgggatcg tcaaggagga gctcggcgag gtcagggcgg
     2461 cctacgcgga cgatcgcagg acggcgatca cggccgagga gggcgtcgac ttcgagatag
     2521 aggacctcat agccgaggag gagatggtga tctccatctc caacgggggt tacctgaaga
     2581 gcgtccccgt caactctttt cgcaagcagg gccgcggcgg ggtcggcgtc gccgggatgg
     2641 agctcaaaga gggcgactat atcgaccacc tctttattac gacgacgcac cattacatgc
     2701 tcttctttac caacaagggc aaggtctaca ggctcaaggt ccaccagctc cctcgcatgg
     2761 gccgggcggc caagggcagg cacatcgcca acatgctgcc cctcgcgcag ggcgagcaca
     2821 tcgcgtccgt tctcgcgacc aaagagttcg gggaggccga gtacctgctc ttcgcgacca
     2881 agagaggcat cgtcaagaag accaggttcc tggagtacaa cacgcccttg aagaacgatg
     2941 ggattatcgc gatcaacctc gccggcgagg acgagttgat ctccgtccgc tacgcctcgg
     3001 agggcgacga ggtcgttatc gtcagccgca acgggaaggg catccgcttc aagcaggcgg
     3061 acgcacgacc gatgggccgc gctacggccg gagtgaaggg cataacgctc ggcaaggacg
     3121 acgtagtgct ctctatggat gtgatctccg acgacgcggc agacctcttc atactcaccg
     3181 aacgcggttt cggcaagcgc acagccctct cgcagtacag ggcccaggcg cgcggcgggc
     3241 agggagttat cgctatgaag accgacggag aacgcggtaa attggccggc gtgcgggtcg
     3301 tccgtcccgg actccacgag ctcatgatcg tctccaactt cgggaccacc atccgcatag
     3361 acgccgactc ggtctcgcga cagggccgcg cggcgcaggg tgtcagggtg atgaacctga
     3421 ggagcggcga ctcggtgagc gccatagcca aagtcatctc ctcccgctcc acccacaccg
     3481 acggcgccac ggacgagctg acgctggatg agctctccgg ggagagtcag acaggcacct
     3541 ctaccgcagg gcttcccgaa gccgacggga acggggcggt gccaggtgca tagcgagtgc
     3601 gaaggtgtgg tcgagtgcac ggcactcgtc ctcgccgaga gctgcacgga gtacgaagat
     3661 aggttgacgc tctttggggt actggatgag atccgcccta acggcgccag cgggacctcc
     3721 ttcgtcgtct acgccaagtt cgagggctgc gaacacgcct cggacaagga gcgccgggcc
     3781 cgcatcctcg tcaccgacga ggacggtgag gtcctgatgg agtcggcgga gtattccgtc
     3841 gacctcgacg gaacagaaaa acccgagacg atagtagccg ccttcgactt acctgaaggc
     3901 ttcgaggtcg gggagcagct actctggatc gaggccgtcc tcgacgagga gacgctctcc
     3961 cagaccgcag tcaggatcca cgaccgctac agtgtataag agacttacag aggcccggat
     4021 ctccgggcct ctcctctacg ttaggaaacc tattcatgtc tgaagatctg gcagtattta
     4081 tcgactggga gaacatctac atctccacag tcacagagta caaatccagg ccgaacgtct
     4141 cggccatact ggagaaggcc cgcgaatacg ggcgcatcgt cgcggccacg gcttacgccg
     4201 actggaccga cggagacttc cgccaggccc cgccgaccct ctactcgaat ggcatctcgc
     4261 cgcgctacat atcggccagg tacttccccg gtggcaagtc tcagaagcgc cgcaccaact
     4321 ccatagacgt catgctcgcg gtcgagtgct cggacttctt gcacaaccac ccgcaggtgg
     4381 acacctacgt cctcgtcact ggcgatgggg acttcatccc gctcgtcagc ctgctcagga
     4441 gcaaggggaa aaacgtggtc gtgatcgggg tctccgaggc gacttcgtac cacctgatcg
     4501 aatcggcaga ccacttcgtc tcctacgcct ccctgctcaa ggaggaccgc gccgcgcgcg
     4561 tccaggatag ggagcccaaa aaggaggccg cggaccccta cgaagagctg gtcagggccg
     4621 tccagacgtt gaagaagggc ggcaagacgc gtgtcctcgg gcaggtgaag cagcagatga
     4681 tcgttcagct cggctctttc gacgagcgga acgcaggctt caagaagttc aaggatttcg
     4741 tggtcgaggc ggagcgccgc ggcctggtaa acacggttga ccaggacctc gagcttcagg
     4801 tctacctgcc cgacgagaag gtgcccgacc tgcaggagac cgacctcgca gccgaggagc
     4861 ccacctcaca gagccaatcg gcaaaccgga acaggggcaa cggcaggccc gcgcgggaag
     4921 agcgtcagca agaagagccg gaaccaggct tcgacctcct cgaagacctc acaccctacg
     4981 agctcaagac ggtctgcacg agcgtcgcct ccctcgaaca gccggccacc ttcgtggaga
     5041 tcttctccag catccgcgat ctggacgagc gcggcgaact ggagctctcg cgggaggaga
     5101 tcggggacgt cataaccgcc atgctcgacg cgaattacct gggcaaagtg cgccagaggc
     5161 cctacaaaca ggcgaaggcc gacatgacct tctacgccct aaacgctgaa aagcaagagg
     5221 tcaagagtgc actgaacagc ggctgactcc gtcgtgcaga aggctctcaa gccttctgca
     5281 cgacgagcac ttcagcattt cagctagcca gctatcgcat gttcaggttc tagctgactt
     5341 gctgacaagg cggcggagcc gccgtgctga catgctgctc tagtcggcca gcttgtagac
     5401 ggtgagggcg gcgaggctga gggcggccgc tatctggacc agagacagcg ctactaccca
     5461 gaccatctgt accgccgaga cgctgctgaa caggatttgc acggcgacta ttgcgccgaa
     5521 gcctatagcg aaccaaagta tcgccctggc cctgtctcct tcactccacg tagccatccc
     5581 gaacagcgcg gggatgtaga acgccaggat caaaggtatc aggcgcagga taccaactcc
     5641 taggtcgagc atcgtctcca tggctaaagt ctacaccata ggttccggga tactggaaaa
     5701 tagatcgcat catatgtacg cttataagct agtacaatta aatagaggtg gtggcgcgtg
     5761 ggtataaatc agagggtttc gctcaggaag ttcatagacg acatccgccg ttgggtggag
     5821 gaggacagga gcgacgagtg gatcgccagc gccctcggaa caagcccttc ttcggtgcag
     5881 tcttttcgct cgcgcaacgc catctaccgc cgcggaggcg gctcgacgct gcacgacccg
     5941 caggagtact cttcctacga aggggtgctc gagccggaag gcccaggcat ctggttcgac
     6001 cccgaagtcg ggaacgaccc gcgctggcag aagcgctgga agagcaccga gagggtcgag
     6061 ctgcgcctca caccgaccag gatggtgctc gtccgccgtg ggggcgctag aagatcctcc
     6121 ctcggctcgt aaggtataat acggcagtcc gggccgtccg acccgtcgct gcatcctcgg
     6181 gctcaagccc agggtgtaga gcaaagtccg gactcgtagc agaggccgct cgggctaagg
     6241 taacccgagg gacggaagag cgcgagccct ccgtcacgtc aagtgcaaca gagagcagac
     6301 cagccgatgg ccgcggttcg ccgtggcaca ggtgatgggt gaaagggtgg ggtaagagcc
     6361 caccggcggc atcccgaggc gaaagccgta gggaccggcc aggtaaacca ccggtgcgag
     6421 caagccaaat tgcccgtggg ggcgtgcgcc ccacgggctg gtaggcgcgg ccccgcgagg
     6481 ggcccgggga ttccccggag atggatggcg ggatagagca gaatccggct tacgggacgg
     6541 ccctgacctt tatgtatgcg acttcctcgt cgcgtagcac gttttgaccc cgcgttttac
     6601 cccaaccaga tcagcaagac taacggcgag tggtcgggaa gctgacagct acgcggtcag
     6661 cgaaggtgtt ttttccgacg tgattgcgct ggcgggtcgg cctcgatagc acctttagcc
     6721 tcgagcttta tcaaagcttc tttgtaatgt ccagccaaga aaggtctgtc tacgctgtgc
     6781 gcctcgtaga tcttgcgcat cgtaagtgtg cggcccgcga agtcgtgaag cagctgacct
     6841 tcgagctcgt ccaacggccg ggatagctcg aagagaa
//
LOCUS       DATMJA010000214         4668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_147500, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000214 DATMJA010000000
VERSION     DATMJA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147500"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..917)
                     /locus_tag="VNT12_05060"
     CDS             complement(195..917)
                     /locus_tag="VNT12_05060"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HWI45523.1"
                     /translation="MRSVVAAVLVPVLVVFLIGPYTFLPSLVEYAVARDVKARLGVEK
                     QPYVKLKSNPPLRMLAGEFSGGRIVMKNTDLGNVTATAVRIDLDPFDINLWATVKKGH
                     VVYRDPLSGDVRVDVPESEVSRLAMAGSEIPITSVDVQEGGVVVRSEVSVLGTHLPVS
                     IEGEPGLRDGELIFEPQGLTAAGIQVPDELADRLLAGTSFEYPLNRLPYQTTITGVEA
                     EEGRIMLSGRVPSIPLGAYPVG"
     gene            complement(922..2013)
                     /gene="prfB"
                     /locus_tag="VNT12_05065"
     CDS             complement(922..2013)
                     /gene="prfB"
                     /locus_tag="VNT12_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWI45524.1"
                     /translation="MTELKDKTSELEERLAELEAFFDVEGLRTEAEELGEDMSRPGFW
                     DDPEEARAVSARFSRVQGRIELLDGLKESLSDSGELLELAEEDRELLPEVREELRRVE
                     RVLEEQEVARLFAGEYDEGDAILTINSGAGGVDSQDWAEMLARMYQRWAERRGFGLEV
                     IEYTEGEEAGIKSATFTVSGEYAFGLLSAERGVHRLVRISPFDAGARRHTSFASVAVA
                     PVVDEAVEVDIEEKDLKVDTYRASGAGGQHVNKTDSAVRITHLPTGIVAQCQNERSQY
                     QNREVAMRVLRARLFELEREKREQEIAAQSGEKADIEWGSQIRSYVLHPYKLVKDHRT
                     GEETANVDRVLDGDLDAFVYSYLKNRTVA"
     gene            complement(2013..4598)
                     /gene="secA"
                     /locus_tag="VNT12_05070"
     CDS             complement(2013..4598)
                     /gene="secA"
                     /locus_tag="VNT12_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWI45525.1"
                     /translation="MANLLTKILRMGEGRKVKVLQQRVEAVSALESEIQKLSDADLRE
                     KTDEFRERLAGGETLDDLLPEAFAVVREVSRRTLGMRPFDEQIMGGIVLHEGKIAEMK
                     TGEGKTLAATMPVYLNALEGKGVHVITVNDYLARRDAAWMGEIYTFLGLEVGLIQESM
                     NFDERKIAYHSDITYGTNAQFGFDYLRDNIATSTDQLVQRELTFGIVDEVDSILVDEA
                     RTPLIISGMPETAADVYYRFAAIVPRLKSGEDYEVDEKKQQVAPTESGVEKVEKALGI
                     ENLYDDVNTNLVNHLNQALRAQTLFQKDDEYIVTDGQVYIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVEIKEENQTVATITIQNFFRMYDKLSGMTGTAATEADEFMHTYKMEVVS
                     IPTHEEMIRDDKDDLVYKTEKSKHKAVVEDIVERNKHGQPILVGTVSVDVSERLSALL
                     NRRGIAHNVLNAKQHEREAEIIAGAGERGAVTIATNMAGRGTDIKLGEGVPELGGLYV
                     LGTERHEARRIDNQLRGRSGRQGDPGESRFYLSFEDELLRLFGGQRMQGIIERIGLEE
                     DVPIEAGMISNSVRRAQEQVESRNFQMRKRILEYDDVLNKQREVIYAIRRDILMGEDV
                     DTKSYVDDVLTEVVSEYVSENVYPEDWDLDALTTEVNRFYPSRIDFKAIDVETLTAAE
                     VLAMVLQDADERLEERKAEWDERTAELERRGLARGDGVATFEEAERRTLLSVVDSRWR
                     EHLYEMDYLREGIGWRGLSQRDPLVEYKREGFDIFQEMERGLKADYVTYIYRVENIKL
                     REEEMQQLSYSGGEELNQRPKSPRKSATKIGRNEPCPCGSGKKYKKCHGQPGAPPLAS
                     EARAG"
BASE COUNT          763 a         1533 c         1366 g         1006 t
ORIGIN      
        1 gagccgctag gccccaccag gaacacgaat tcgcctgaat ctatccctag gttcacacgc
       61 tccaaagcga cggtatcgtt accgtagact ttggtgacgt tgtcgaatcg gatcattact
      121 tctccatgcg cggttagagg tcccccctcc agggtcccgg ccaccggaga cgataacaac
      181 aaccgcaggc gttatcaacc gacggggtac gcgccgagcg gtatgctcgg caccctgccg
      241 ctgagcatga tccgcccttc ctcggcctcg acgccggtga tggtggtctg gtagggcaaa
      301 cggttaaggg ggtactcgaa tgacgtacct gcgagcaacc ggtctgcgag ctcatcaggc
      361 acctgaatcc ccgccgccgt gaggccctgc ggctcgaaga tcagctctcc gtcgcgcaag
      421 ccaggctccc cttctatgga aacaggtaag tgcgtgccga gcaccgagac ctccgacctg
      481 acgacgactc cgccttcctg cacgtccacg ctcgtgatgg gtatctcaga tcccgccatc
      541 gccaggcgcg agacttcgct ctcgggcaca tcgacccgta catctcccga tagggggtcc
      601 ctgtaaacca cgtggccctt ttttacggtg gcccagaggt ttatgtcgaa ggggtcgagg
      661 tcgatgcgca ccgcggttgc cgttacgttg cccagatcgg tgttcttcat gacgatgcgg
      721 ccgcccgaga actcgcccgc cagcatgcgt agcggggggt tgctcttcag ctttacgtag
      781 ggctgctttt cgaccccgag acgggccttg acatctcggg cgaccgcgta ctccacaagc
      841 gaaggcaaga atgtgtatgg tccgatcagg aagacgacga gtacgggcac gagcacggcc
      901 gcgacaacgc tccgcatagc gctaggccac ggtcctgttc ttcaggtaag agtagacgaa
      961 ggcatcgagg tcgccgtcga gcacgcggtc cacgttcgcc gtctcttcac cggtgcgatg
     1021 gtcctttacg agcttgtaag ggtggagtac gtaggatcgg atctgcgaac cccactcgat
     1081 atccgccttc tctccactct gcgcggcgat ctcctgctcc cgcttctcgc gctcgagttc
     1141 gaagagccgc gccctgagta cccgcatggc cacctcacgg ttctggtact ggctgcgctc
     1201 gttctggcac tgcgccacga tgcccgttgg gaggtgcgta atcctgacgg ccgagtcggt
     1261 cttgttgacg tgctggccgc cggcccctga ggccctgtat gtatccacct tgaggtcttt
     1321 ctcctcgatg tcgacctcga cggcctcatc gacgacgggt gcgacggcga cggaggcgaa
     1381 gctggtgtgc ctccttgcgc cggcatcgaa aggactgatt ctgacgagac gatgcacccc
     1441 gcgctcggcg gataagagcc cgaaggcata ctcccccgag acggtgaagg tggcgctctt
     1501 tatgcctgcc tcctcgcctt ctgtgtactc gatcacctcg agcccgaagc cccgacgctc
     1561 agcccagcgc tggtacatgc gcgccagcat ctcggcccag tcctgcgaat ccaccccgcc
     1621 cgcgcccgag tttatggtga gtatggcgtc tccctcatcg tactcgccgg cgaagagcct
     1681 ggcgacctct tgctcctcga gcacccgctc gacccgcctg agctcctctc tgacctcggg
     1741 gagcaactct cggtcctcct cggcgagctc gaggagctcc cccgaatccg agaggctctc
     1801 tttaagcccg tcgagaagct cgatcctgcc ctgcacccgg gagaagcgtg ctgacaccgc
     1861 cctggcctcc tcaggatcgt cccagaaacc aggccggctc atgtcctcac cgagctcttc
     1921 ggcctctgtg cgcaaccctt ccacgtcgaa gaacgcctcg agctcagcga ggcgttcttc
     1981 cagctcggat gtcttgtcct tgagctctgt cactaacctg cccgggcttc ggacgcgagc
     2041 ggcggcgcgc ccggctgtcc gtggcacttc ttgtacttct tgccgcttcc gcacggacag
     2101 ggctcgttgc gcccgatctt ggttgcactc ttgcgcgggc tcttgggacg ctgattcagc
     2161 tcttcgccgc cgctgtagga gagctgctgc atctcctcct cgcgcagctt gatgttctcc
     2221 acacggtaga tgtaggtgac gtagtccgcc ttcaggcccc gctccatctc ctggaagatg
     2281 tcgaaaccct cccgcttgta ctcgaccagc gggtcacgct gggagaggcc gcgccagccg
     2341 attccctcgc gcaagtagtc catctcgtag aggtgctccc gccagcggga atcgaccaca
     2401 gagagcagcg tccggcgctc ggcctcctcg aaggtggcta ccccgtcgcc gcgtgcgaga
     2461 ccgcgcctct ccagctcggc ggtccgctcg tcccactcgg ccttgcgctc ctcgaggcgc
     2521 tcgtcggcgt cttggaggac catcgcgaga acttcggcgg ccgtaagagt ctcgacatcg
     2581 atagctttga aatcgattct gcttggatag aagcggttca cctcggtcgt gagggcgtcc
     2641 aggtcccagt cctcggggta gacattctcg gagacgtatt cagagacgac ttctgtaagc
     2701 acgtcgtcca cgtaggactt cgtgtcgacg tcttcgccca tcaagatgtc gcgccttatc
     2761 gcgtagatca cctcgcgctg cttgttgagg acgtcgtcgt actcgagtat gcgcttgcgc
     2821 atctggaagt tgcggctctc gacctgctcc tgggcacgcc tcacggagtt ggagatcata
     2881 ccggcctcga tgggcacatc ctcttcgagc ccgatacgct cgatgatccc ctgcattcgc
     2941 tggccgccga agagcctcag gagctcgtcc tcgaaggaca agtagaacct cgactcgccg
     3001 gggtcgcctt gccttcccga gcggcccctc agctgattgt cgatacggcg tgcctcgtga
     3061 cgctcggtgc cgaggacgta gagcccgccg agctcgggca ctccttcgcc gagcttgatg
     3121 tctgtaccgc ggcccgccat gttcgtagct atggtcacgg cgccccgctc gccggccccg
     3181 gcgatgatct ccgcctcccg ctcgtgctgc ttggcgttga gtacgttgtg cgcgatacca
     3241 cgccggttta gcagcgccga gaggcgctcg gagacatcca cggagaccgt gccgacaagg
     3301 atgggttggc cgtgtttgtt tcgctcgacg atgtcctcga ctacggcctt gtgcttggac
     3361 ttctcggtct tgtagacgag gtcgtctttg tcgtccctga tcatctcctc gtgcgtcggg
     3421 atggagacga cttccatctt gtaggtgtgc atgaactcgt cggcctcggt cgcggccgtg
     3481 cccgtcatgc cggagagctt gtcgtacatc ctgaagaaat tctggatggt gatggtggct
     3541 acggtctggt tctcttcctt gatctcgacg ccttctttgg cctcgatggc ctggtggagc
     3601 ccctcggagt aacgcctgcc ctcgagcaca cgccccgtga actcgtcgac tatgtagacc
     3661 tgcccgtcgg tgacgatata ttcgtcgtcc ttctggaaga gcgtctgcgc cctcagcgcc
     3721 tggtttaggt ggttgacgag gttggtgttg acgtcgtcgt agaggttctc tataccaaga
     3781 gccttctcga ccttctcgac accgctctcg gtcggtgcta cctgctgctt tttctcgtcc
     3841 acctcgtagt cttcgccgct cttcaggcgc ggcactatgg cggcgaatcg atagtagacg
     3901 tcggcggcgg tttccggcat gcccgagatg atgagcgggg tgcgcgcctc gtcgaccagg
     3961 atggagtcga cctcgtccac tatcccgaag gtgagctccc gctgcacgag ctggtcggtc
     4021 gaggtcgcta tattgtccct taggtagtcg aagccgaact gggcgttcgt gccgtaggtg
     4081 atgtccgagt ggtaggcgat cttccgctcg tcgaagttca tactctcttg gatgaggccg
     4141 acctcgagac ccaagaaagt gtagatctca cccatccagg ccgcgtcgcg gcgggccagg
     4201 tagtcgttga cggtgatgac gtggacgcct ttgccttcca gggcgtttag gtaaacaggc
     4261 atggtggccg ctagcgtctt gccctcgccc gtcttcatct ccgcgatctt gccttcgtgc
     4321 aacactatgc cgcccatgat ctgctcgtcg aaggggcgca tcccaagggt acgcctggag
     4381 acctcccgca cgacggcgaa agcctcgggc agcaaatcat cgagggtctc gccgccggcg
     4441 agcctctcgc gaaactcgtc cgtcttctcc cgcaggtctg cgtctgaaag cttctgtatc
     4501 tcggactcca acgccgagac cgcctcaaca cgctgctgga ggaccttgac cttgcgacct
     4561 tcgcccatcc tcaatatctt cgtcaacaag ttcgccaaaa caagctacct cgtagtagtg
     4621 gtcgtaccgg actaacgggc aggctctatg agcccgtagt ctccgtca
//
LOCUS       DATMJA010000215         2611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_562252, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000215 DATMJA010000000
VERSION     DATMJA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562252"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VNT12_05075"
     CDS             <1..378
                     /locus_tag="VNT12_05075"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWI45526.1"
                     /translation="DVGRAVYEAEAHAGTGGTGEVACSGDLLLGALAACAQVTCQMVA
                     EAMGIEAESIEVTVAGEMDLAGTLGVSDDVPVGFETIRTTFDIVAPEATEEQLEDLRQ
                     KTEQYCVVFQTLAETPDLQTDWA"
     gene            393..1337
                     /gene="meaB"
                     /locus_tag="VNT12_05080"
     CDS             393..1337
                     /gene="meaB"
                     /locus_tag="VNT12_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWI45527.1"
                     /translation="MDGKDGLVKRLLAGDRRALARVISKIEREDEETAGIIAEIYPET
                     GDALTVGFTGPPGVGKSSIIAKLIELYRKEDRRVGVVSVDPSSPFSKGAILGDRIRLT
                     DHFLDAGVFIRSMGSRGHLGGLAAGSRLAAIAMEAGGYDVIFYETVGVGQGEVEVASA
                     ADTVVLCLQPGTGDSVQALKAGVMEIADVFCINKADNPQARNAASEVRSILEIGHELD
                     PDPWFPPIVMTRGDTGEGVQELKDTIVKHRRFLEQDGRLGKRRRAALRDFVISLATNR
                     LEIEMHERLNCEDTELVEKVYNRELDPISASEIIFKEV"
     gene            complement(1350..1823)
                     /locus_tag="VNT12_05085"
     CDS             complement(1350..1823)
                     /locus_tag="VNT12_05085"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HWI45528.1"
                     /translation="MRSGALRTLGALGCGIVFGLGLAISGMMNPAKVIGFLDVAGDWD
                     PTLALVMGGALLVAIPAYRVILKRRRPVLSGGFSLPAEEKLDAPLIWGSALFGVGWGL
                     VGFCPGPAVAAIVTVLPAVLGFVAAMIAGMALHARFSGELTRRKSSSGDTAPNPR"
     gene            complement(1820..2242)
                     /locus_tag="VNT12_05090"
     CDS             complement(1820..2242)
                     /locus_tag="VNT12_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HWI45529.1"
                     /translation="MGNFTPVSGLVGGLLIGLAVALMMLLNGRIAGISSIVGGLVTPK
                     SGDTAWRFAFVVGLLLGALAFILAVGGPTPVDVLASPPAILIGGLLVGFGTRMGSGCT
                     SGHGLCGIARFSRRSIVATSVFFGVAMLTVFLTYHVLL"
     gene            complement(2327..>2611)
                     /locus_tag="VNT12_05095"
     CDS             complement(2327..>2611)
                     /locus_tag="VNT12_05095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI45530.1"
                     /translation="PRACYSGVAEFSVYTASEARGRGAGTLAMRALMQAAAEAGFWKL
                     VARVFVENTPSRKLLLTLGFREVGIHEKHARLDGVWRDVVVVERLLPANM"
BASE COUNT          539 a          795 c          857 g          420 t
ORIGIN      
        1 gatgtaggcc gcgccgtcta cgaggccgag gcccacgcgg gcacaggcgg tacaggagag
       61 gtcgcttgtt ccggagatct tctgctgggc gcgttggcag cctgtgccca ggtaacctgt
      121 cagatggtcg cagaggccat ggggatagag gcggaaagca tcgaggtcac cgtggcaggg
      181 gagatggacc tcgccgggac gctcggcgtc tccgatgacg tccccgttgg cttcgagact
      241 atccgcacca ccttcgacat cgtcgcgccc gaggctaccg aagaacagtt ggaggacctg
      301 cgccagaaaa cagaacagta ctgcgtcgtc ttccagacgc tggccgagac accggatctc
      361 cagacggatt gggcgtagaa gatcgaaccg gcttggacgg caaggatggg ctcgtaaaac
      421 ggttgctcgc gggcgaccgg cgggcgctgg cgcgagtgat ctcgaagata gagcgcgaag
      481 acgaagagac ggccgggata attgcagaga tctacccgga gaccggcgat gctctgaccg
      541 taggcttcac ggggcctccc ggcgtcggaa agagcagcat catcgccaag ctcatagaac
      601 tttacaggaa ggaagacagg cgggtcgggg tggtctctgt cgacccttcg agccctttct
      661 cgaagggtgc tatcctcggt gaccgcataa ggctcacgga ccacttcctc gacgccggag
      721 tgttcatccg ctcgatgggt agccgcggac accttggtgg gctcgccgcc ggctcgcgac
      781 tcgccgccat cgcgatggag gccggcggct acgacgtgat cttctacgag accgtagggg
      841 ttggacaggg agaggtcgag gtagcctctg cggccgacac cgtggtcctt tgcctgcagc
      901 cgggtacggg tgactcggtg caggcgctca aggccggggt catggagatc gcggacgtct
      961 tctgcatcaa caaagcggat aaccctcagg caagaaacgc cgcctcagag gtgcgttcta
     1021 tactggagat cggacacgaa ctcgaccccg acccgtggtt cccgcctatc gtgatgaccc
     1081 gaggcgatac aggcgagggc gtccaggaac tgaaagacac catagtgaag caccgtcggt
     1141 tcctcgaaca ggacggcagg cttggcaaga gacggcgggc cgcccttaga gacttcgtca
     1201 tttcgttggc cacgaaccgc ctggagatag agatgcacga gcgcctgaac tgtgaggata
     1261 cagagctcgt cgagaaggtc tacaacaggg agctcgaccc gatctcagcc tcggagatca
     1321 tcttcaagga agtgtagagc ttcgtacgtc taacgagggt ttggggctgt atcgccggac
     1381 gaactcttgc gccttgtgag ttcgccggag aaccgggcgt gtagtgccat gccggcgatc
     1441 atggcagcga cgaacccgag caccgcagga agtaccgtta cgatagcggc gaccgcggga
     1501 ccggggcaga agcccacgag gccccagccc acgccgaaga gggctgagcc ccagatcagg
     1561 ggcgcgtcga gcttctcctc tgcaggcagg gagaaaccgc ccgagaggac gggacgtcgc
     1621 cgtttcagga tcacgcggta cgccggtatg gcgacgagta gcgcgccgcc cataaccaag
     1681 gcgagagtag ggtcccagtc gccggccacg tcgaggaagc ctattacctt ggcagggttc
     1741 atcatcccgg agatggcgag accgaggccg aacacgatgc cgcagcctag tgcacctagt
     1801 gtccgtaggg cgccggacct cataagagga cgtggtaggt caggaacacg gtgagcatcg
     1861 ccaccccgaa gaatactgat gtggcgacga tcgaacgccg ggagaagcgc gcaatgccgc
     1921 aaaggccgtg gccgctcgtg cagcctgagc ccatgcgggt gccgaagccg accaggagac
     1981 ccccgatcag gatagctggg ggagacgcga gtacgtccac cggagtcggc cctcccaccg
     2041 ccaggatgaa tgccagggcg ccgagaagga gtcccacgac gaaagcaaaa cgccaggccg
     2101 tgtccccact ctttggtgta acaagaccac cgacgatgct gcttatcccg gcgatacggc
     2161 cgttcagcag catcatcagc gcgaccgcga gcccgatcaa aagcccgccg acgagcccgc
     2221 ttaccggcgt aaagttcccc acccttgtct ccgttcgtag atctctgtcc cggacagcac
     2281 gaggatagca cgcttgtcgc gcggtagggc gcttcgcgag gcgcccctac atgtttgccg
     2341 gaagaagtcg ctcgacgact acgacgtcgc gccatactcc gtccagcctg gcgtgtttct
     2401 cgtggattcc cacctccctg aagcctaaag tgagtagtag cttccggctc ggggtgttct
     2461 ccacgaagac acgtgcgacg agtttccaga agcctgcctc cgccgccgct tgcatgaggg
     2521 ccctcatcgc cagggtgccc gctccacggc cccttgcctc gctggcggtg tatacggaga
     2581 actcggccac gccggagtag cacgcgcggg g
//
LOCUS       DATMJA010000216         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_680704, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000216 DATMJA010000000
VERSION     DATMJA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680704"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VNT12_05100"
     CDS             complement(<1..889)
                     /locus_tag="VNT12_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45531.1"
                     /translation="MKTQLELNIRSRGAMSGVLTMGSLDPAFAAATSVDGEPVVVDLR
                     TVEFVEPAGLCALAALLEYLCARCEVVDLALPGRDVPAYLERMNFFRSFENRVRTNAD
                     VASLEERRRHNPGTLQELINFHAEEEIPGIIERISEILENQGYRLRERVAICSTLSEV
                     CANAAEHGVSSFGAYAAVQAYHHIVSGSRRRGEEVLIAIADGGVGVRETLSRNPKYGE
                     YTTTDNDALRHALEMGVSATGEIGRGGGLALVAGIAARAGGSLSLRSGAGRVTVYENR
                     KNSRNVPDFPGTFVRVSLPR"
     gene            complement(1037..1534)
                     /locus_tag="VNT12_05105"
     CDS             complement(1037..1534)
                     /locus_tag="VNT12_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI45532.1"
                     /translation="MILRPPRESDVEVVYSWDRDPELAAWNGRSPISISLSAARRDYL
                     ARWEDPSVKTFIIEARGEPIGMATLYDFRHDGCELGIKIGPGNLRGRGYATEAVTLLV
                     DYAFHTLGLAAVRGSTLAHNDRMQRVFEKNGFVRTGDGSLLSRYDNRRYTELFYERRQ
                     EDHKP"
     gene            1686..>2525
                     /locus_tag="VNT12_05110"
     CDS             1686..>2525
                     /locus_tag="VNT12_05110"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45533.1"
                     /translation="MSGTVGEFFGGSSRRVRDDIWGDVAIDRAVRSLLETGAMQRLRG
                     MSQLGFTFGSFPRARHTRLDHSVGVYYLVGLTLKRIAESGAYLEDRDVRAALAAALLL
                     DAGRYPYAASVEGIKVPGAPTLKELSRRWIEHTEVAKVLRAEWDLEPHNVFRLVSRGD
                     TPPHSLTPTEHLLRDILFGSLNVDALDRLIRDARGARVPYGLVEVESLIQRLRIVGQE
                     NKAILALEEEGAGTLQSVVFSRYLVYYNVYGHHALRIPTVMFLRAVQDAIGAGNVTFD
                     ELSE"
BASE COUNT          450 a          756 c          845 g          474 t
ORIGIN      
        1 tacgtggcag ggagacgcgt acgaaggtac ctgggaaatc cgggacgttg cgggagtttt
       61 tacggttttc gtagacggta acgcggcctg cgccggacct cagggagagg gagccgcctg
      121 cacgggcggc gatgccggcg acgagggcga ggccgccgcc gcggccgatc tcgccggtgg
      181 ccgagacgcc catctcgagg gcgtggcgga gggcgtcgtt gtctgtggtc gtatattcgc
      241 cgtacttcgg gttacgggag agggtctctc gcactcccac gccgccgtcg gcgatcgcta
      301 tcaggacctc ctcgccgcgg cggcgggacc cgctgacgat gtggtggtag gcctggacgg
      361 ccgcgtaggc cccgaaggag gagacgccgt gttctgcggc gttagcgcac acctccgaga
      421 gcgtggagca gatcgcgacc cgctcgcgca ggcggtagcc ctggttctcc aggatctccg
      481 agatcctctc gatgatccct ggtatctcct cctcggcgtg gaagttgatc aactcctgca
      541 gcgtccccgg gttgtgcctt ctgcgctcct ccagagaggc cacatcggcg ttggtgcgta
      601 cccggttctc gaaagacctg aagaagttca tacgctccag ataggccggc acgtcgcgtc
      661 ctgggagtgc aagatccacc acctcgcagc gggcgcacag gtactcgagt aacgctgcga
      721 gagcgcagag tcctgccggc tcgacgaact cgaccgtccg caggtccacg accacaggct
      781 ctccgtccac gctggtggcg gcggcgaacg cggggtcgag gctgcccatg gtaagcaccc
      841 cgctcatcgc gccccggctc ctgatgttca gctctagctg cgtcttcatc agccttattg
      901 tagtcggtta gggcaagata gcgaatggga agctgtgcct gggcattgcg gtagccggca
      961 ttttgctact ggagaatggc gccctggtag cggggactca gtagacgtct gtggcgattc
     1021 cggcggagat cttaccttac ggcttgtgat cctcctgccg gcgttcgtag aagagctcgg
     1081 tgtagcgacg gttatcgtag cggctcagta gggaaccgtc accggtccgc acaaagccgt
     1141 tcttttcgaa gacgcgttgc atgcggtcgt tgtgggccag tgtcgagcca cggacggctg
     1201 cgaggccaag ggtgtggaag gcgtagtcga cgaggagggt aacggcttcg gtggcgtagc
     1261 ctctaccgcg aaggttcccg ggaccgatct ttatgcctag ttcgcagccg tcatgacgga
     1321 agtcgtagag ggtggccatg ccgatcggct cccctcgggc ttctatgata aaagtcttga
     1381 cgctcggatc ctcccagcgg gccagataat cgcgacgggc cgccgatagg gagatggaga
     1441 tgggtgagcg gccattccag gcggcgagct ccgggtcacg atcccaggag tagaccacct
     1501 ccacgtcgga ctcccgcggc ggcctgagga tcaccgactc cccctgtaga gcctttacct
     1561 cttctatgcg tatggtgtct cctctctcaa gatacagaat gttacacccg tcgccagccc
     1621 gaggcatcct ttcggaagtg tacacattgg ttagaatagc cgagagcagg agatcgggag
     1681 cgtcattgag cggtacggta ggggagttct tcgggggctc tagcagaaga gtaagggacg
     1741 atatctgggg ggatgtcgct atagatcggg cggtgaggtc gttgctcgag acgggcgcga
     1801 tgcaacgcct gaggggcatg agccagctcg gatttacctt cgggtccttc ccgagggccc
     1861 gtcacacgcg cttggatcac tcggtcggcg tctactacct cgtggggctg acgctaaagc
     1921 ggatcgcgga gtcaggcgcc tatctcgagg accgcgacgt gagggccgcg ctggccgccg
     1981 ccctgctgct agacgccggc cgataccctt acgccgcttc cgtcgaaggg atcaaggtgc
     2041 caggcgcgcc caccctcaag gagttgagca ggcgttggat cgaacacacc gaggtggcga
     2101 aggtcttgag ggcagagtgg gacctcgagc cccacaacgt cttcaggctg gtctcgcgcg
     2161 gggatacacc gccgcacagc ctgacgccca cagaacacct gctaagggac atcctgttcg
     2221 gctcgctgaa cgtggatgcc ctcgaccgcc tgatacgcga cgccagggga gccagggtac
     2281 cctacggcct tgtcgaggta gagtccctca tacagcgcct gaggatcgtg ggccaggaga
     2341 acaaggctat actggcgctg gaagaagaag gggcgggcac gctgcaatcc gtcgtcttct
     2401 cccgctacct ggtgtactac aacgtctacg gccaccacgc cctgcgtatt ccgaccgtga
     2461 tgttcctcag ggccgtccag gacgccatag gggctggtaa cgtgaccttc gacgagctct
     2521 ccgag
//
LOCUS       DATMJA010000217         3435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_266139, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000217 DATMJA010000000
VERSION     DATMJA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266139"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..1154)
                     /gene="pyrF"
                     /locus_tag="VNT12_05115"
     CDS             complement(315..1154)
                     /gene="pyrF"
                     /locus_tag="VNT12_05115"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWI45534.1"
                     /translation="MQALVDAARRKQSVLVAGLDPIPSRFPEELRTLPEAEATLQFCV
                     GILEAVEPYVAAVKLQAAFFERLGTVGMGIYAGLIEAATDLGLPAIADVKRGDIGPVA
                     VAYAGAHLGVYGATCVTVNPYMGKDAVLPFLEEARRQNAGGGVFVLVATSNPSSREVQ
                     EITDPPLFEVVAGLVAGFGRSAGDYPDAGAVVGVTRPEIGRRVRELLPYALFLSPGYG
                     AQGGDASGVRQLLDGGGGGVLVNSSRSILFAYESSASGYREAAKEAAREARDELRLTG
                     ARL"
     gene            complement(1237..1515)
                     /locus_tag="VNT12_05120"
     CDS             complement(1237..1515)
                     /locus_tag="VNT12_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWI45535.1"
                     /translation="MALNKTALIEEIAGKANASKSDAQRFFDAFTEVVTDSLKNGEQV
                     QITGFGKFYVQRRDARQGINPQTKQRINIPASKVPKFTAGNALKDQIK"
     gene            complement(1592..2125)
                     /gene="pyrE"
                     /locus_tag="VNT12_05125"
     CDS             complement(1592..2125)
                     /gene="pyrE"
                     /locus_tag="VNT12_05125"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWI45536.1"
                     /translation="MSRVPDLAPRIRDAALLEGDFVLSSGERSSFYVDKYLFSTEPHL
                     LRDVADALAAEIPGGVERLAGVELGAVPLVVATALATGLPYVIVRKSAKEHGSSAGRS
                     IEGNLEKGERVALLEDVVTTGTQAVRAARNLEEMEAEVVTIVAVLDRREENRGKLGGF
                     PFRSLLKMDDLRVVKRN"
     gene            complement(2109..2477)
                     /locus_tag="VNT12_05130"
     CDS             complement(2109..2477)
                     /locus_tag="VNT12_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45537.1"
                     /translation="MVGTSTGLPGAEDGAGMVYSPEPMEDRGKQQDDLGGLEEKVDEL
                     EKLTDSLEDVPDEELVGTLGEAVELLAEINAGIEARLDSAGDESREIGALLGQVDFGP
                     FDEAMEDHEHRERTTGEPGA"
     gene            complement(2570..2830)
                     /locus_tag="VNT12_05135"
     CDS             complement(2570..2830)
                     /locus_tag="VNT12_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWI45538.1"
                     /translation="MQRQTKSEVLRRLKSVEGHVRGVEKMVEEGDVTYADIVQQTNAI
                     FSAIRRINTVLLKDFVEERAERDGASEEMINDLIKAIDRVTK"
     gene            complement(2839..>3435)
                     /locus_tag="VNT12_05140"
     CDS             complement(2839..>3435)
                     /locus_tag="VNT12_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HWI45539.1"
                     /translation="GPYNVLNVAGAASLALAVGTDETAVSNAVRGMGQVPGRFERVAA
                     AGEYGFEVVVDYAHTDVGLDAVLRVARRVAGSRGRVICVFGAAGDRDGAKRPKMGRVA
                     SLLADMSLITTDDAYSEDPEKIAREVEAGSDLSRTSIELDRRKAIRRALEAARAGDVV
                     VVAGKGHERVQHLPEGDIPFHDATVVRELLAEIGDRGR"
BASE COUNT          573 a         1205 c          979 g          678 t
ORIGIN      
        1 aggctgttcg tggtcgagga cggtacgata gggtacgccc aggactacta ttacgaaggc
       61 tacgagaaca ctctcagaga ggccatcgac gaagccctcg cgaatgagcc ggggaagggc
      121 cgccagagcg ccctccgcga acaggagccc agatcatagc cacgggcgga aggagaagat
      181 gagcagagag agcatggtgc aggttcttgg ggtcgtggtc gcggctgcga cggtgctagt
      241 gaccgtgctt gcggtcgccc acctcttttc tatttgatcg cggttcgcct gtaggggcgg
      301 tttcaaaccc gcccctacag gcgggctccg gtcaggcgta attcatcccg cgcttcgcgg
      361 gcggcttctt tggccgcttc cctgtaacct gaggccgagc tttcataggc gaagaggata
      421 ctccggctgc tgttgacgag gactccgccg ccgccaccgt cgagcagctg gcgcacgcct
      481 gaggcgtcac ctccttgggc cccgtatcct ggggaaagga acagggcata gggcagcaac
      541 tccctcaccc tgcgcccgat ctcaggcctc gtgaccccta cgaccgcccc cgcgtccggg
      601 tagtcgcctg ccgaccttcc aaaccccgcc accagcccgg caacaacctc gaagagcggc
      661 gggtccgtga tctcctggac ctcacgggag gacgggttcg aggtcgccac aagcacgaag
      721 acaccccctc ccgcgttctg cctgcgggcc tcctcgagaa acgggagcac cgcatccttc
      781 cccatgtaag ggttgacggt gacacaggtt gcgccgtaga cgccgagatg cgccccggcg
      841 taagcgacgg ccacggggcc gatgtccccg cgctttacat ccgctatggc aggcaagccc
      901 aggtctgtgg cggcctcgat caagccggca tagatcccca tcccgacggt gcctagccgc
      961 tcgaagaaag cagcctgcag cttgacggcg gcgacgtaag gctccaccgc ctcgaggatg
     1021 ccaacgcaga actgcagggt cgcttcggcc tcgggcaggg tgcgcagttc ttccgggaag
     1081 cgtgagggta tggggtcgag gccggcgacg agcacgctct gcttgcggcg cgccgcgtct
     1141 accagcgcct gcatcgtgcc ctggctggtt gacaaacggg cgagaagaat ggtgccgcga
     1201 ggagaagccc gcggcaccag aaaagcccct gggcagctac ttgatctggt ccttgagcgc
     1261 gttgcccgcg gtgaacttag gcaccttgga tgctggtatg ttgatcctct gcttggtctg
     1321 cgggttgatc ccctgcctgg cgtcgcgcct ctgaacgtag aacttgccga agcccgtgat
     1381 ctgaacctgc tcgccgttct tgaggctgtc cgtcacgacc tcggtgaacg cgtcgaagaa
     1441 cctttgggcg tcgctcttgg aggcgttcgc cttgccagcg atctcctcta tcaatgccgt
     1501 tttgtttagc gccactactt cctccctact tcgcttccac tccacttccc tccggtctgc
     1561 cggaaggatc taccagagac actatagcgt ctcaattccg tttaactacc cgtaaatcat
     1621 ccattttaag caaggaccgg aaaggaaatc cgccgagctt ccccctgttc tcctccctgc
     1681 ggtcgagtac tgctactatc gtcacgactt cggcctccat ctcttcgagg ttcctggcgg
     1741 cccgcacggc ctgcgtaccc gtggtgacga cgtcctcgag aagggctacc ctctcgccct
     1801 tctccaggtt gccttcaata ctgcgtccgg ccgagctgcc atgctctttg gcagacttgc
     1861 ggacgattac gtagggcaac cctgtggcga gggctgtggc aacaacgagg ggaacggcac
     1921 cgagctccac gccggcgagc cgctctactc cccccggtat ctctgccgcc agcgcgtcgg
     1981 ccacgtcgcg caggaggtgc ggttcggtcg agaagaggta cttgtccaca tagaagctgc
     2041 tgcgctctcc gctggagagt acgaagtccc cttccaggag cgccgcatcc ctgatgcggg
     2101 gtgcgagatc aggcacccgg ctcaccggta gtcctctccc tgtgttcgtg atcctccatg
     2161 gcctcgtcga agggcccgaa gtctacctgc ccgaggagcg cgccgatctc gcgcgactcg
     2221 tcccccgcgc tgtcgagcct ggcttcgatg ccggcgttga tctcggccaa tagttcgaca
     2281 gcctcgccta gggtcccgac cagctcttca tctgggacgt cctcgagaga gtcggtgagc
     2341 ttctcgagct cgtcgacctt ctcctccaga cctcccagat cgtcctgttg cttccctcga
     2401 tcctccattg gttccggact atacaccatc ccggccccat cttcggcccc cggaagaccc
     2461 gtgctcgttc ctaccatgac tgccctttgc ggccctgctc aacacgaagg cgagcgcagc
     2521 gttcatcaac gagcaagccg cgaaaggcgc aacaccccgc cccctacctt cacttcgtca
     2581 ccctgtctat ggcctttatc aggtcattta tcatctcttc ggaggcgcca tctcgctcgg
     2641 cccgctcctc gacgaagtcc ttcaagagga ccgtgtttat gcgcctgata gccgagaaga
     2701 tggcgttcgt ctgctggacg atgtcggcgt aggttacgtc gccttcttcg accatcttct
     2761 cgacccccct gacatggcct tccacgctct tgagccggcg tagcacctcg ctcttggtct
     2821 gcctctgcaa cgcctgcgct acctccctct atcccctatc tccgccagta gctccctgac
     2881 caccgtggcg tcgtggaagg gaatgtcccc ttcagggagg tgctggaccc tctcgtgtcc
     2941 cttgccggcc accaccacga cgtcaccagc cctcgccgcc tccagcgcgc gccggatcgc
     3001 cttccgcctg tcgagctcga tgctggtgcg tgagagatcc gagccggcct cgacctctct
     3061 tgctatcttc tcagggtcct ccgagtaggc gtcgtccgtc gtaatgaggc tcatgtcggc
     3121 gagaagcgag gccactcgcc ccatcttcgg ccgcttcgcc ccgtccctgt ctccggccgc
     3181 cccgaacaca cagatgactc gccccctgct accggctacc ctcctggcca cccgcaggac
     3241 agcatcaagc ccaacgtcgg tgtgggcgta gtctacgaca acctcgaacc cgtactcccc
     3301 ggccgccgcg acccgctcga agcgtccagg aacctgtccc atcccccgca ctgcattgct
     3361 taccgccgtt tcatccgtcc ccacagcgag cgcgagcgag gcggcccccg cgacgttgag
     3421 cacgttgtaa ggtcc
//
LOCUS       DATMJA010000218         3524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_88373, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000218 DATMJA010000000
VERSION     DATMJA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88373"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNT12_05145"
     CDS             <1..210
                     /locus_tag="VNT12_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWI45540.1"
                     /translation="TFWYVEALAWAGRSEEAQLVFEKMLTYANHLGLYAEEIGPSGEA
                     LGNFPQAFTHLALISAAFNLDRQLG"
     gene            complement(949..1671)
                     /locus_tag="VNT12_05150"
     CDS             complement(949..1671)
                     /locus_tag="VNT12_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWI45541.1"
                     /translation="MPEIFMAEAIRREALRAREAASANDAVSYYDGIMADDPDALVRS
                     FAARQSVLDDPRVGHVEGARRLRAFAIGTAEWLRERDAVVENVALTRTSTRTVEEVVL
                     NLLADDGGRVELPVTIVTDRNPDRMLKAIRVYHSTWPLTGEHRLRPPLLPVNPGLHAV
                     GTPGEYQRALAEGDLEGVLATFESEGYARAPSGAAYLHRGTEALRELYTHMFANAGGI
                     GLEHCTLTDDGVRCAIEYDCVP"
     gene            complement(1811..2743)
                     /locus_tag="VNT12_05155"
     CDS             complement(1811..2743)
                     /locus_tag="VNT12_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWI45542.1"
                     /translation="MAFRSVGTNLSRPYTDGVSGLAEDLEALKSAGPDFVEVWPQNLG
                     VILGGSLDANRLRTVGELLLEAELAYTVHAPLEVNLMDLSTHGLQRDVLNSSLRFAKD
                     IGAEVVVCHAGQRVAARDARHSMQDQLSAERSALREAGDLAGELGVTIAVENYYPERP
                     IVRGAIYDYSVWPSQLAEQIFAVEHPAVGICLDVGHAALAAGFFGFDFIEECGATASL
                     VRHVHLHDNLQRTNVTGEVQASEHTVYGLGDLHLPPGRGTIPLEDLFRGMEFPENPSC
                     CVELSPELFSLVPEALHAAREISLVVAAREAVSL"
     gene            2882..>3524
                     /locus_tag="VNT12_05160"
     CDS             2882..>3524
                     /locus_tag="VNT12_05160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI45543.1"
                     /translation="MNRGGKVELSSYEDYTRKSGNYDKTREPVGTEILVGCFAHAPVP
                     LDRTVVLDAGCGTGSYSDALLGYVGRIEAVDLNPGMLEVAARKLAWAGDRISFHSSRI
                     EELPFEDANLDGVMINQVLHHLPDAAAEGFPAHRRVFREFARVLKPGGILTISTCSQE
                     QLRHGYWHYNLIPEAADALRNRYVPLDELLEILDDCGLAHRGSFVPTDATVQGE"
BASE COUNT          618 a         1139 c         1100 g          667 t
ORIGIN      
        1 acgttctggt atgtcgaggc gttggcgtgg gccggacggt ccgaagaagc acagctggtc
       61 ttcgagaaga tgcttaccta cgctaatcac ctgggactct acgccgagga gattgggccc
      121 tcaggcgagg cgctgggcaa ctttccgcag gccttcaccc acctcgccct catcagcgct
      181 gccttcaacc tcgaccgcca gctaggttga cgacgaaacc tgtactcggc aagggtgctg
      241 actggatccg acgcgacgaa tggtactgag cagctcactt gtggtacgca gattccaaga
      301 taccggctta cctgagcgtc atcgacgctg atctcttaac gcgtcttcca tcgcgctacc
      361 tgcgacctct cccagcctcg gcattacatg gctgtgggtc tatgatcctg catagcccgg
      421 ttctttggtc ttcaagagct cgcctagccc cgtcgaatac ccaactctaa gcactacatg
      481 ttgtggtgcc gtatggatat gcactcgagt gcatggcttc ggccttctgt gcccgtgagc
      541 tgctagtcga gaactacgta aagcacctcg ccgattctaa ccgctcgacg ccgctcttgg
      601 agagtagggc gcgaaacctg agttctcccg tgtttgtagt ttggcaacaa tacggtcact
      661 aagcgttcgg ttagggagcg ctcgacaata ctctgtaagg ctcgctaagt gcaatcgtgt
      721 atccgttccg ctaccgcttc gcgtctattc acccgagtgc gtggaaggga tgttctccga
      781 tgttagactt ccgctttacg gagttctcgg aagttccgac tagctcatgg aggtgtccga
      841 gacactcggg ggttccacgt cgtcgtagat gcgcgctgcc gccagcagcc cgctagcact
      901 gcgctcgtag atcgccaccc ccgcctgcgg cgggatatcg gtcgtcccct acggcacgca
      961 gtcgtactct attgcgcacc tcaccccgtc gtcggtgagc gtgcagtgct ccagcccgat
     1021 gccgcccgcg tttgcgaaca tgtgcgtgta gagctcccgc agcgcttcgg tgccacggtg
     1081 gaggtacgcc gcgccgctgg gcgcgcgggc gtagccctct gactcgaagg tcgccaagac
     1141 accttccagg tcgccctcgg ccaaggccct ctggtactct cccggcgtgc ctacggcgtg
     1201 cagccccgga ttgacgggca gaagcggcgg gcgtaggcgg tgctcgcccg taagcggcca
     1261 cgtgctatgg tagacgcgga tcgccttcag catgcgatcg gggttgcggt cggtgactat
     1321 ggtcacgggc agctccaccc gcccgccgtc gtcggccaag aggttcagca caacctcctc
     1381 gaccgtgcgg gtggaagtac gagtgagggc tacgttctcg accaccgcgt cgcgctcgcg
     1441 cagccactcg gccgtcccta tggcgaaggc gcgtagccgc ctcgcgccct cgacgtgtcc
     1501 tacgcgggga tcatcaagga cggactggcg tgcggcgaag gagcgcacta gagcatcggg
     1561 gtcgtctgcc atgatgccgt cgtagtacga gacggcgtcg ttggcgctcg cggcctctct
     1621 tgcgcgtagc gcctcgcgcc ggatcgcttc tgccatgaag atctctggca tccagggcaa
     1681 aggagcctcc tctgagtagg gtcacgtacc gcgcccgtac ccacaaagcc tacgctattc
     1741 agcagagtgc gtggaaggag gattcttcga acgccctcct tcgctcgcgg gagaccggtc
     1801 ggcccggtgc tcagagggac actgcttctc gcgctgctac caccaggctg atctctctgg
     1861 cggcgtgaag ggcctccgga accagggaga agagttcggg ggagagctct acgcagcacg
     1921 aggggttttc ggggaactcc atcccccgga acaggtcttc cagcgggatg gtgcccctgc
     1981 cgggtgggag gtgcaggtct cccagcccgt agacggtgtg ctcggaggcc tgcacctccc
     2041 ccgtaacgtt ggtcctctgc aggttgtcgt ggaggtgtac gtggcggacg agggacgctg
     2101 tagcgccgca ctcctctata aagtcgaagc cgaagaagcc cgcagcgagg gccgcgtgcc
     2161 ccacgtccag gcagatgccg accgcggggt gctcgaccgc gaagatctgc tcggccagct
     2221 gggaaggcca gaccgagtag tcgtagatgg cgccgcgcac tataggccgc tcggggtagt
     2281 agttctcgac ggcgatggtc acgccgagct ctcccgccag atctcccgcc tcccgcaggg
     2341 cggaccgttc ggcggagagc tgatcctgca tgctatgcct tgcgtcgcgc gctgccacgc
     2401 gttggccggc gtggcagacc accacctcgg cgccgatgtc cttcgcgaac cggaggctgg
     2461 agttgaggac gtcgcgctgc aggccgtggg tgctcaagtc catgaggttg acctcgagcg
     2521 gggcatggac cgtgtacgcc agctccgcct ccagcagcag ctccccaacc gtccgcagcc
     2581 tgttcgcatc caggctaccg ccgaggatga ccccaaggtt ctggggccag acctccacga
     2641 aatccggtcc ggcgctcttt agcgcctcga ggtcctcggc gagcccgctg acgccgtctg
     2701 tatacggccg ggacaggttg gttcccacgc tccgaaaggc catacggttc cttcctccct
     2761 tagggccgcg cgcagtatag gctcgacgag cctcccttag gtgaaaagaa ggttaatgat
     2821 gagtaaaagt tgggtttaac cggctctgtc agatgcgtcc atatactctt acgaggaaaa
     2881 gatgaaccga gggggaaagg tcgaattgag cagctacgag gactacacgc gcaaatccgg
     2941 caactacgac aagaccaggg agcctgtagg gaccgagatc ctcgtcgggt gcttcgccca
     3001 cgcgcccgta ccattggacc ggacggtggt gcttgacgcc gggtgcggca ccggcagcta
     3061 ctccgacgcg ctgctcggtt acgtcggccg gatcgaggcg gttgacctca accccggcat
     3121 gcttgaggtc gccgcgcgca agctggcgtg ggctggggac cgcatctctt ttcactcctc
     3181 caggatcgag gagctgccct tcgaggacgc gaacctggac ggggtgatga tcaaccaggt
     3241 gctccaccac ctgcccgacg ccgccgccga agggttcccc gcccaccgcc gggttttccg
     3301 ggagttcgcc cgggttctaa agccaggcgg tatcctcacg atcagcacct gctcgcaaga
     3361 gcaactgcgc cacggctact ggcattacaa ccttattccg gaggccgccg atgctctcag
     3421 aaaccgctac gtacccctcg acgagctgct cgaaatcctc gacgactgcg gcctcgccca
     3481 caggggaagc ttcgtgccca ccgacgccac ggtccaaggc gagt
//
LOCUS       DATMJA010000219         3601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_325786, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000219 DATMJA010000000
VERSION     DATMJA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325786"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VNT12_05165"
     CDS             <1..573
                     /locus_tag="VNT12_05165"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWI45544.1"
                     /translation="HEVVSNASCTTNCIIPLVKVIQDNFGIESGYMTTVHAYTNDQSL
                     LDAAHKDPRRARSAPNNIVPTSTGAARTAGVIYPELQGKVDGMAMRVPVPDGSITDFV
                     ARINNEASADDVNAAFKEAAEGELSGVLEYSEAPLVSSDIVGNIASCVFDSQLTMSNG
                     GTVKVLGWYDNEWGYSNRTCDLAAYMGDKL"
     gene            723..1895
                     /locus_tag="VNT12_05170"
     CDS             723..1895
                     /locus_tag="VNT12_05170"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWI45545.1"
                     /translation="MNKRSVRDLDVGDKRVLVRVDFNVPVRDGEVTDDTRIRRALPTI
                     RYLLEERARPILISHLGRPKGEPDPKYAMDPVAARLKEVLGEPVVKLDAAVGPEVEEA
                     IDDWDGKGVVLLENSRFYPGETSDDPGFADQLAALADLYVNDAFGAAHRAHATTVGVA
                     ERLPAAAGLLMEEEIDYLDKVLEDPERPFVAILGGAKVSDKIGVIESLLGTADSLLIG
                     GAMGFTFFQALGYEVGDSLVEEDYLEEAKRLMEEAGDRLVLPVDVVVAETMEEGAESE
                     TVATGGIPSGKMGLDIGPETVDLFEGHISGASTIFWNGPMGVFEIDAFAKGTEGVARA
                     VAKSGATSVVGGGDSVAAVNKLGLEDQMSHISTGGGASLEYVEGKELPGIAVLPDK"
     gene            1911..2696
                     /gene="tpiA"
                     /locus_tag="VNT12_05175"
     CDS             1911..2696
                     /gene="tpiA"
                     /locus_tag="VNT12_05175"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWI45546.1"
                     /translation="MARRPMMAANWKMNKTVREAQEYTAALLPRAADAEEDVDVVVCA
                     PHTCLHEVGRMAGDSAVIAGAQNFYYEDSGAYTGEVSAPMLLDVGAGAVIVGHSERRQ
                     VFGETDEMVARKTKRAVEADLLPIVCVGETKEERDAGGMWDKVSDQVGRVVEELEDVA
                     GESIVFAYEPIWAIGTGDTATPEDAQDAIGKIRDLLTETKGDDFAESVRILYGGSVKP
                     ENAAEIMAQEDIDGALVGGASLEVESFVELIGAAQNAVARTEQ"
     gene            2718..2945
                     /gene="secG"
                     /locus_tag="VNT12_05180"
     CDS             2718..2945
                     /gene="secG"
                     /locus_tag="VNT12_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWI45547.1"
                     /translation="MLYYIVAFFQVVFCIALVGLILLHSGKGGGLSSSLGGGMGSTFS
                     GTTIMEKNLTRLTLIVVGLFLLTNVTLFFMG"
     gene            complement(3326..3413)
                     /locus_tag="VNT12_05185"
     tRNA            complement(3326..3413)
                     /locus_tag="VNT12_05185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3377..3379),aa:Leu,seq:gag)
BASE COUNT          746 a         1047 c         1180 g          628 t
ORIGIN      
        1 cacgaggtcg tctccaacgc ctcgtgcacg accaactgca ttatcccgct ggtcaaggtg
       61 atccaggaca acttcgggat cgaatcaggg tacatgacga ccgtgcacgc ctacaccaac
      121 gaccagagcc tgctggatgc ggcccacaag gacccgaggc gggcgaggtc cgctccgaac
      181 aacatcgtgc caacgtcgac cggtgcggcc cggactgccg gtgtgatcta ccccgagctg
      241 cagggtaagg tggacgggat ggcaatgcgc gtccccgttc ccgacggctc cataactgac
      301 ttcgtcgccc gcatcaataa cgaggcatcc gccgacgacg tgaacgccgc cttcaaggaa
      361 gcggccgaag gagagctctc cggcgtcctc gagtactcgg aggccccgct ggtctccagc
      421 gacatcgttg gaaacatagc ctcctgcgtc ttcgactcgc agctcaccat gtccaacggc
      481 ggtaccgtca aggtgctcgg ctggtacgac aatgagtggg gctactcgaa cagaacgtgc
      541 gacctggcag catacatggg ggacaagctc taggagcttg tcagcaagtc agcacggcgg
      601 ctccgccgcc ttatcagtaa agacctgccc gtgcgaaagc tgacaagctg aaatgctgac
      661 gagcgaagcc cgaagggctg agcgtgctga cttgctgaaa tgctgccgac gaaggaggca
      721 ccatgaacaa acgcagcgtc agagacctcg acgtggggga caaaagggtg ctggtccgcg
      781 tggatttcaa cgtgcccgtc agggatggcg aggtcacgga cgacacgcgc atccggaggg
      841 cgctgccaac gatcaggtat ctccttgagg agagggctcg cccgattctg atctcccacc
      901 tcggccgtcc caagggggaa cctgacccta agtacgccat ggacccggtc gccgcgagac
      961 tcaaggaagt gctcggagag cctgtagtga agctcgacgc cgctgtcggc cccgaggtgg
     1021 aagaggccat cgatgattgg gacggaaagg gcgtggtgtt gctcgagaac tcccggttct
     1081 atcccggcga gaccagtgac gatccgggat ttgcagacca gctcgcggcc ctggcggacc
     1141 tctacgtgaa cgacgcattc ggcgcggccc acagggcgca cgccacgacc gtcggcgtgg
     1201 ccgagaggtt acccgcggct gcggggctct tgatggagga ggagatcgac tacctcgata
     1261 aggttctcga agaccccgaa cgccccttcg tcgccatcct cggaggggcg aaggtctcgg
     1321 acaagatcgg tgttatagag agcctgctcg ggaccgcgga ctcactcctc atcggtggcg
     1381 ctatgggctt cacgtttttc caggcgcttg gctatgaggt cggagattcc ctcgtcgagg
     1441 aagattacct ggaggaggct aagcgcctta tggaggaggc cggcgacaga ctcgtcctgc
     1501 ccgtggacgt tgtcgtagcc gaaaccatgg aggagggtgc cgagtccgag acggtcgcca
     1561 caggtggcat cccgtcgggc aagatgggtc tcgacatcgg cccggagacc gtcgatctct
     1621 tcgagggaca catctcgggt gctagcacca tcttctggaa cggcccgatg ggcgtcttcg
     1681 agatagacgc cttcgcgaag ggcacggagg gcgtggccag ggccgtcgcc aagagcgggg
     1741 cgacgagcgt ggttggcggc ggagattctg tcgcagccgt caacaagctc ggactcgaag
     1801 accagatgag tcacatctcg accggtgggg gggcttcgct cgaatacgta gaaggcaagg
     1861 agctgcccgg catagcagtc ctgcccgaca aatagaacag ggaggagatc atggcccgac
     1921 gtccgatgat ggccgcgaac tggaagatga acaagaccgt cagggaggcc caggagtaca
     1981 cggccgccct cctcccgcgt gccgcggatg cagaggagga tgtggacgtg gtggtctgcg
     2041 cccctcatac ttgtttgcat gaggtgggga ggatggccgg agactctgcg gttatcgcag
     2101 gcgcgcagaa cttctattac gaggactctg gggcgtatac cggcgaggtc tcggccccca
     2161 tgctcctcga cgtcggggca ggagccgtga tcgtgggcca ctcagaacgg cgtcaagtct
     2221 tcggcgagac cgacgagatg gtcgccagga agacgaagcg tgcggtggag gccgacctcc
     2281 tacccatcgt ctgcgtcggg gagaccaaag aggagcgcga cgcagggggt atgtgggata
     2341 aggtctcgga ccaggtcggg cgggtagtcg aagagctcga agacgttgcc ggcgagagca
     2401 tcgttttcgc ctacgagccg atatgggcca tagggacagg cgatacagcc actcccgagg
     2461 atgcgcagga cgccatcggt aagatcaggg acctgctgac ggagacgaaa ggagacgact
     2521 tcgcagagag tgtccgtatc ctctatggcg gctccgtcaa gccggagaat gcggctgaga
     2581 tcatggccca ggaagacatc gacggcgccc tggtaggcgg agcgagtctc gaggtcgagt
     2641 ccttcgtcga gctgataggg gccgcgcaga acgccgtcgc gcgtacagag cagtaacgcg
     2701 tgtggtaagg tagcagcatg ctgtactaca tcgttgcttt ctttcaagtc gtattctgca
     2761 tagccctggt gggcctgatc ctcctccact caggaaaggg aggcgggctc tcttcctcgc
     2821 tcggcggcgg aatgggcagt acgttttcgg gcaccaccat catggagaag aacctcacca
     2881 ggttgaccct gatcgtcgtt ggactcttcc tgctgaccaa cgttaccctg ttcttcatgg
     2941 gttaaagcat ttcagcacgc cgccttcggc gacttatcag cacgctccgg cttccgcttg
     3001 tcagcacttc agcaagtcgt cccgtagcct cgccaccagc gcagaacctg accgcgatca
     3061 gcaatggttc ctcgtgcgcg cctgggcgaa cctggaacgg cagaagctga aatgctgaca
     3121 agcgagggcc gctaggcccg aagcgtgctg acaagcggag gcgaagccgg agcgtgctga
     3181 ctagctgaga caaggactcg cccggggagg atggggagaa caggcgagtc cagagtaatt
     3241 atacacccca gcgtatccct ccgcgtgcat gaggttacaa ggttagtggg ctgtttacat
     3301 tcgatgcggg tgcacgaggc gagcctggtg tcggaggggg gactcgaacc cccaccccct
     3361 catcggggac taggccctca acctagcgcg tctaccaatt ccgccactcc gacagggtag
     3421 cccgcagagt atacgagaga cacatagtgg ggtcaacgat agtggggtca acgccgcacg
     3481 tccccctctc cactatgcta agttctcctg atgcgcagaa agcacttggt cttgatctcc
     3541 ctcgcagggt tggcttgctt tgtcgccctg atcttgggga gggtaagaaa gaggactgtc
     3601 c
//
LOCUS       DATMJA010000220         8097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_858750, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000220 DATMJA010000000
VERSION     DATMJA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858750"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VNT12_05190"
     CDS             <1..863
                     /locus_tag="VNT12_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI45548.1"
                     /translation="SFLLLGEDGAAAALVETGPTNCLDRLTAGLKDRGVSYEDVRQVY
                     LTHIHLDHAGASGHLSGVLPNATFYVHEVGYPHLADPSKLVKSATRIYGERMEELWGE
                     ARPVPEDRIKVLKDGEETETVGGVLVAHDTPGHAYHHLAYLEPGSGALFTGDVAGIRL
                     PGQSYVRPPTPPPEINVKAWLQSINYLRRIDPASLWPTHFGSYEDVERHLGELEQRLQ
                     DWLLLVEGQMDDGADREEISDQLKAKGDAEMLAEGSDIIQSGRYDLSGTYSMLTDGLM
                     RYVTRQREKR"
     gene            941..1297
                     /locus_tag="VNT12_05195"
     CDS             941..1297
                     /locus_tag="VNT12_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWI45549.1"
                     /translation="MARSMILIAVIVVILGGLFFLLRPDTPSGVPRDRTIAVSIEGGE
                     MSPAAISVNEDDSVTLRVSSDRPMELHLHGYDVEQEVGPGQKASLRFRADLTGRFEIE
                     DHESEKELGVLQVRPR"
     gene            1303..2811
                     /locus_tag="VNT12_05200"
     CDS             1303..2811
                     /locus_tag="VNT12_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45550.1"
                     /translation="MQTRRVAALALALSLILAGARPALAHGFGPTYDIPIPLWLYLYG
                     AAAAVVLAFLPLALFSRKERDADAPYRYPRFDLFRVRLMRTILTSRLLTGGLRLLSVT
                     LFFVVVIAGLVGLQSGFNIAPTFVWVTWWVGLSFFTAFVGNLWPLVNPWRVIFDWAEG
                     LARRLGYRDGLDEPYPEALGIWPAVGLYLVFVWIENVFSGSYVPRNIALFALAYTLIT
                     LYGMAFFGKETWLRRGEAFSVFFGLLGRFAPTEVRVKDPDVCPDCDGCGDDARGRCVN
                     CYECFARAAPDERALNLRPPAVGLRLPEKVPPGGTAFVVVVLAGVTFDGLLQTPLWLE
                     IVRLTPVTQTLGTILLPLVFLGIYLGFVELSRILGGGEVGFRRLAAAYTFSLVPIALA
                     YQMAHYYTYLLIQGQMIISLVSDPLGWGWNLFGTADFEPRYGIVGAGFVWYSQVALIV
                     AGHVIAVYLAHSISLRLLRDPGRALRSQLPMLVLMVLYTMTSLWILAQPIVK"
     gene            2920..3924
                     /locus_tag="VNT12_05205"
     CDS             2920..3924
                     /locus_tag="VNT12_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI45551.1"
                     /translation="MNGEAGNPHTGLEIGRTLRLVREKRGLSLQQVEEATKIRTRYLR
                     DLENENFDVLPAVYMLGSLKTYAEHLGLDGAAITRELKRRLASLQPEQDQAAEDPASG
                     EPRGFLTSLGRLVGITGTVGDEADTMAPVRRPGLYVSLAVVLIFVLATYLASSNWGED
                     LPSVSVVQEPTVSQLPSGIALVGNVEDEERNTEDPNKENQPKNQAESPTIDTGNDERT
                     RAERSGLEEYSSQTAQVSSSATVVASASASASASASPASPGSASASATATPPPTRVRP
                     EPAAREQPGGGDMAAPGGLSARVSGSQSLHETQAGPVDATRLGDGVPIKVKKAVDSAR
                     "
     gene            complement(4085..5497)
                     /locus_tag="VNT12_05210"
     CDS             complement(4085..5497)
                     /locus_tag="VNT12_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWI45552.1"
                     /translation="MQVAPFLAYLAAAALFIYGIRRLRTPETARSGNTLAAVGMVIAL
                     IATIFVADIDQVLSAGEIVGGVLVGSVVGAVAAQRVHMTAMPQMVAAFNGVGGGAAAL
                     IAVSEFYRVEALGQGQETLVSSIAVLFSVIIGTVSFAGSIIAFTKLQELALSGSVTFP
                     LQQVVNAAIFLGILLLSANILAGGLVLSPLVSLYLILGASLLLGVLFVIPIGGADMPI
                     VISLLNAFTGLAAAASGFVLSNYALIISGTLVGASGTILTRLMSGALGRPLGKIIFAS
                     ITATGSSSGEQQEDREVNDASASEVGEYLADEAEKVVIVPGYGLAVAQAQQALRDFVE
                     TLESEGKEVLFGIHPVAGRMPGHMNVLLTEVGIPYEKLYDLEDINPEFDDADAAIIVG
                     ANDVVNPSANDPEVESPISGMPVLTVENAEHVIFIKRSLSPGFAGVDNPLFYDTEKTM
                     MLFSDAKQGLQDLNETVKNG"
     gene            complement(5500..5808)
                     /locus_tag="VNT12_05215"
     CDS             complement(5500..5808)
                     /locus_tag="VNT12_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HWI45553.1"
                     /translation="MTSQLLAQLAIFVISAFLGFELISRVPNLLHTPLMSATNAIHGI
                     VLVGAIIALASASDGFTTTVGLIAVVFGAMNVVGGFWVTNRMLGMFGPPKKRVKKREE
                     "
     gene            complement(5805..7097)
                     /locus_tag="VNT12_05220"
     CDS             complement(5805..7097)
                     /locus_tag="VNT12_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HWI45554.1"
                     /translation="MKVGIPREVAEGERRVGLVPETVGRLVKDGFEVLVESGAGRDYN
                     LDGAYEEAGASIAEGPEEVYGEADIVVKVQSPVEDEIGLLREGEVLICFLNGPQEPEL
                     VEKLAEAGVTVFSNEAVPRTSAAQAMDALSSMGSIAGYKCALIAADTIGKYVPMLSTA
                     AGTTKAANVMVFGVAVAGLQAMAIMNRLGAEVFAYDIRPETKEQAQSLGATFIDSEDE
                     KEGEEEEDGYEEYKPTGWRKLMSSLGFYSYAEPPRDRYIVEGEEEEDADSKGEDEGWS
                     QEKLDEDQKLIRERMEEMDIVITTALVPGRKAPQLVDKDMIESMKPGSVIVDLAAESG
                     GNCELTEAGETVEHKGVRIVGPVNLPSTMPIHASQLYSRNMYNLIGHITEDRSEDKDE
                     KDLHLDLDFEDEITGETCIAHGGEIRHGPTREALQEAG"
     gene            7247..7915
                     /locus_tag="VNT12_05225"
     CDS             7247..7915
                     /locus_tag="VNT12_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWI45555.1"
                     /translation="MKKQSAAYQRRLAALVVAVFAFAELGLLGYSLVAGGSPASAESP
                     DPVHGRLSEELENKFHRSIPERIVETVRGPQAKTPESKRLELTIPALDRVDGVPVYDA
                     SESGYEKALHDGIVHVRGTGFPWHNVANVYIAGHRVGYPGTKSNLVFWDLDKLEKGDE
                     VFLTDADGTRYTYEVFEKRVVSPDTVSIMRPIKGRNIVSLQTCTLPDYSHRLIVQGEL
                     KGRN"
BASE COUNT         1522 a         2541 c         2471 g         1563 t
ORIGIN      
        1 cctcgtttct cctgctcggc gaagacgggg ctgccgccgc cctcgtcgag accggtccga
       61 cgaactgcct cgaccggctc accgccggcc tgaaggatcg tggcgtctcc tacgaagacg
      121 tgcggcaggt ctaccttacg cacatacacc tcgaccacgc aggcgcctcg ggacatctct
      181 ctggggtgct gcccaacgcg actttctacg tgcacgaggt cggctacccg caccttgccg
      241 acccttcgaa gctagtaaag agcgccaccc gcatctacgg cgagaggatg gaggagctct
      301 ggggcgaagc acgccccgtg cctgaggaca ggatcaaagt cctgaaagac ggggaagaga
      361 cggagaccgt aggaggcgtc ctcgtcgccc acgacacgcc gggtcatgcc taccatcacc
      421 tcgcctacct ggagcctggc tccggggcgc tctttaccgg tgacgtggcc ggtatccggc
      481 tgcctggcca gtcctatgta aggccgccga cgccgccgcc cgagatcaac gtcaaggcgt
      541 ggttacagag catcaactat ttaaggcgga ttgatccagc aagtctctgg cccacacact
      601 tcgggagcta tgaggatgtg gagcggcatc tcggcgagct cgagcagcgc ctgcaggact
      661 ggttgctgtt agtcgagggg cagatggacg atggcgccga ccgcgaggag atctccgacc
      721 agttgaaggc caaaggggat gccgagatgc tcgctgaggg ctccgatatc atccagtcgg
      781 ggcgctacga cctgtccggt acctacagca tgctaaccga cggtctcatg aggtacgtga
      841 ccaggcagcg cgagaaacgt tgacggcgcg cccctccact acagtcgagc tgggatcgtg
      901 acgaggatag ggaccaggcc ttactgagaa tagagggacg ttggctagga gcatgatcct
      961 catcgcggtg atcgtagtga tcctgggcgg cctgttcttc ctgctgcgcc ccgacacccc
     1021 ctccggcgtg ccgcgcgacc ggacgatcgc cgtctccatc gagggcggcg agatgagtcc
     1081 ggccgcgata tccgtcaacg aggacgacag cgtgacgctc cgggttagct ctgataggcc
     1141 gatggagcta cacctgcacg gctacgacgt tgagcaagag gtcgggccgg gacagaaggc
     1201 cagccttcgc ttcagggcag acctcacggg acgcttcgag atcgaagacc acgaatccga
     1261 gaaggaactc ggcgtactac aggtgcgccc ccggtagtag acgtgcagac ccgccgcgtc
     1321 gcagccttag cgctcgcgct ctcactcatc ctggccggcg cccggcctgc gctagcccat
     1381 ggcttcgggc cgacctacga tatcccgatc ccgctctggc tctacctcta cggcgcggca
     1441 gcagcggtgg tgctcgcctt cttgccgctg gccctgttct cccgcaagga acgggacgcc
     1501 gacgccccct accggtatcc gcgcttcgat ctgtttcggg tccggctcat gaggacgatc
     1561 ctgacctcac gcctgctcac aggcggcctg cgcctgctct ctgtcacgct cttcttcgtc
     1621 gttgtgatcg ccggcctggt tggtttgcag agcggcttca acatcgctcc gactttcgtg
     1681 tgggtgacgt ggtgggtggg gctcagcttc tttaccgctt tcgtcggaaa cctgtggccg
     1741 ctcgtgaatc cctggagggt gatcttcgac tgggctgaag ggctggcccg ccggctaggc
     1801 tacagggacg ggctcgacga gccttacccc gaagctctcg gcatctggcc cgctgtcggg
     1861 ctctacctcg tgttcgtctg gatcgagaac gtcttctcgg gttcgtacgt gccgcgcaac
     1921 atcgccctct tcgctctggc gtacaccctg atcacactct acggcatggc cttcttcggc
     1981 aaggagacct ggttgcgtcg gggagaggcg ttctctgtgt tcttcggcct gctcggcagg
     2041 ttcgccccca cagaggtaag ggtgaaggat ccggacgtct gcccggactg cgatggctgt
     2101 ggcgacgatg caaggggaag gtgcgtcaac tgttacgagt gcttcgcccg tgccgctccc
     2161 gatgagcgcg cattgaacct gcgccctccg gcggtcggtc tcagactgcc cgagaaggtg
     2221 cctccgggag ggaccgcgtt cgtcgtcgtg gtgctggccg gcgtgacatt cgacggcctg
     2281 ctccagaccc cgctgtggct cgagatcgta cgtcttaccc ctgtgacgca gacgctgggg
     2341 acgatattgc tgcccttggt gttcttaggc atctatctcg gctttgtgga actgagccga
     2401 atcctcggcg ggggagaagt aggtttcagg cgtctcgcgg ccgcctacac tttctcgctc
     2461 gtacccatag cgctcgccta ccagatggca cactactaca cgtacctgct catccagggg
     2521 cagatgataa tctccctcgt ctccgaccct cttggatggg gttggaacct cttcggcacc
     2581 gccgatttcg agccccgtta cggcatcgta ggggctggat tcgtctggta ctctcaggtc
     2641 gcgctcatcg tggccgggca cgtgatcgcc gtctatctcg cacactcgat ctcgctgcgc
     2701 ctcttgcgtg accctggacg cgccttgagg agccaacttc cgatgctcgt cctgatggtc
     2761 ctctacacga tgaccagcct ctggatcctg gcccagccca tcgtcaagta ggggcggttc
     2821 gcgaacgaat atagtcccgt gcgctcttgg gtgtggtaca ttccaagata ttagaatggt
     2881 ctacccgggg ggagcagact tagagggacc tgggtgagca tgaacggtga ggctggcaac
     2941 ccgcatacgg gcctagagat aggcaggacc ctgcggctgg tccgcgagaa gcgaggtctg
     3001 tcgctccagc aggtcgagga ggcgacgaag atccggacca ggtacctgcg agacctggag
     3061 aacgagaact tcgacgtgct gcctgctgtg tacatgctcg gctccctcaa gacctacgcc
     3121 gagcaccttg gactggacgg agcggcaata accagggagc tcaaacgccg cctggcgtcc
     3181 ctgcaaccag aacaggacca ggcagcagaa gatcctgcgt cgggcgagcc gcgtgggttc
     3241 ctgacatccc tgggccgtct cgttggcatc acagggacgg tcggagacga agcagacacg
     3301 atggcgcccg ttcgcagacc cggcctgtac gtgagccttg cggtggttct gatcttcgtc
     3361 ctggccacct acctcgcttc aagcaattgg ggcgaagatc taccatcggt ctcggtggta
     3421 caagagccga cggtctccca gcttccctcc gggatcgccc tggtcggcaa cgtggaagac
     3481 gaggaacgta ataccgagga tccgaacaag gagaaccagc ccaaaaatca ggccgaatct
     3541 cccactatag acaccggtaa tgacgaacga accagggccg agcgtagcgg gctagaggag
     3601 tactcatccc aaacagcaca ggtgtcatcc tctgctactg tagtcgcgag cgcgtcagca
     3661 agcgcgtcag cgagcgcgtc acccgcttcc cccggatctg cctccgcgtc tgcgacggcc
     3721 acgccgccgc caacaagggt tcgaccagaa ccggcagcca gggaacagcc cggcggggga
     3781 gacatggctg ccccgggtgg tctaagtgcc cgcgtgtccg gcagtcagag tcttcacgag
     3841 acgcaagcgg gtcccgtcga cgccacccgg ctcggcgacg gggttcccat caaagtgaag
     3901 aaagctgtgg actccgcccg gtgacctcgc cggagatgtc tcgaaagccg ttcatgggag
     3961 tgcaccgact ctcccatcac atattagact attagcgaga ggctgcatgc gaaaacaaag
     4021 ggctgacaag ctgaagtgct gactagctga gagtatacaa agaggtttgg gcctctttgt
     4081 aaacctagcc gttcttcacg gtttcgttga ggtcctgcaa gccttgcttg gcgtcggaga
     4141 agagcatcat ggttttctcg gtgtcgtaga agagggggtt gtcgacgccg gcgaagcctg
     4201 ggctaagtga gcgcttgatg aagattacgt gctccgcgtt ctcgacggtg agcacaggca
     4261 ttccgcttat ggggctctct acctcagggt cgttggccga agggttgacc acgtcattgg
     4321 cgccgacgat gatcgcggcg tcggcgtcat cgaactcggg gtttatgtct tcaaggtcgt
     4381 agagcttctc gtaggggatg ccgacctcgg tcaagaggac attcatgtgt cctggcatcc
     4441 ttcctgcgac tgggtggata ccgaacagca cttccttgcc ctcggactcc agcgtctcca
     4501 cgaagtctcg cagagcctgc tgggcctggg cgacggcgag accgtaaccg ggtacgatca
     4561 caaccttctc cgcctcgtca gcgaggtact cgccgacttc cgaggcgctc gcgtcgttga
     4621 cttcccggtc ttcttgctgc tcgcccgaac tgctgcccgt ggcggtgatg ctggcgaaga
     4681 tgatcttgcc tagaggacgc cccagggcgc ccgacatgag cctcgtcagg atggtccccg
     4741 aggcgccgac cagcgtaccg ctgataatca gggcgtagtt ggagagcacg aagcctgagg
     4801 cagccgccgc gagcccggtg aacgcgttta acaacgagat cacgatgggc atgtccgccc
     4861 cgccgatggg gatcacgaac agaacgccaa gcaagagcga agcgcccagg atcaaataca
     4921 gagagacgag cggcgagagc acgagtccgc ccgccaggat gtttgcggag agaagcaaaa
     4981 taccgagaaa aatagcggcg ttcaccacct gctgcaaagg gaacgtcacc gagccgctca
     5041 gggccagctc ctggagcttg gtgaacgcga taatgctccc agcgaagctc accgtcccga
     5101 taatgacgct gaagagcacc gcgatggagg agacgagcgt ctcctgtccc tggcccagcg
     5161 cctcgacccg gtagaactcc gagactgcta tgagtgccgc tgcgccgccc ccgacaccgt
     5221 tgaacgccgc gaccatctgc ggcatcgccg tcatgtgtac ccgttgggcc gctaccgccc
     5281 ctacaaccga cccgaccaag accccgccca cgatctcacc agcgctcagt acctggtcta
     5341 tgtcggcgac gaagatggtg gcgatgagcg ctatgaccat gccgactgcc gccagggtat
     5401 tccccgaacg ggccgtctcg ggcgtcctca agcgacggat gccgtagatg aacagcgcgg
     5461 cagccgcgag gtaggccaaa aacggtgcga cctgcaaggt tattcctctc tcttcttgac
     5521 ccgcttcttc ggcgggccaa acatgccgag catcctgttc gtaacccaga aaccgccgac
     5581 aacgttcatc gccccgaaga cgacggctat aaggccgacg gtcgtcgtga atccgtcaga
     5641 agctgaggct agcgcgatga tagcgcctac cagcacgatc ccgtgtatgg cattcgtcgc
     5701 gctcatgagc ggcgtatgga gcaggttcgg cacgcgggag atcaactcga agcccaggaa
     5761 cgccgagatt acgaaaatcg ccaattgggc gagaagttgt gaggtcatcc ggcctcctgg
     5821 agtgcttctc tggtcggccc gtggcggatc tcgcccccgt gcgcgatgca cgtctcgcct
     5881 gtgatctcat cctcgaagtc aagatcgagg tgtaggtctt tctcgtcttt gtcctctgag
     5941 cggtcttcgg tgatatgccc gatgagattg tacatgttgc gcgaatagag ctggctggcg
     6001 tgaatgggca tcgtgctagg caggtttacg ggaccaacga tcctgacccc tttgtgctcg
     6061 acggtctctc cggcctcggt aagctcacag ttcccgccgc tctccgccgc gaggtccacg
     6121 atcacggacc ctggtttcat gctctcgatc atgtccttgt ccacgagctg tggagctttc
     6181 ctacctggta cgagcgcggt cgtgatgacg atgtccatct cctccatgcg ctccctgatg
     6241 agcttctggt cctcatcgag cttctcctgg gaccaccctt cgtcttcccc tttggagtca
     6301 gcgtcctcct cctcttcgcc ctcgacgatg taccggtccc ggggcggttc ggcgtacgag
     6361 tagaagccaa gtgaggacat cagcttccgc caccctgtgg gcttgtactc ctcgtacccg
     6421 tcttcctcct cttcgccttc cttctcgtcc tcggagtcta tgaacgtggc gccgagtgac
     6481 tgcgcttgct ctttggtctc gggcctgatg tcgtaggcga acacctcggc cccgagacgg
     6541 ttcatgatcg ccatggcctg caagccggcg accgccaccc cgaataccat gacgttcgcc
     6601 gccttcgtcg taccagcggc ggtggagagc atggggacgt acttgccgat ggtgtcggcc
     6661 gcgatgagcg cgcacttgta gccggcgatg gagcccatcg aggacagagc gtccatggcc
     6721 tgcgccgcgc tggtgcgtgg aacagcctcg ttcgagaaga ccgtcacgcc cgcttcggcg
     6781 agcttctcga ccagctcagg ctcctgcggc ccgttcagga agcagataag gacctcgccc
     6841 tcgcgcaaga gcccgatctc atcttccacc gggctctgca ctttgactac tatgtcggcc
     6901 tcgccgtaca cctcctcagg gccttcggcg atgctcgctc ccgcctcctc gtacgcaccg
     6961 tcgaggttgt agtcgcggcc ggcgcccgac tcgacgagaa cctcgaaacc atccttgacg
     7021 agcctgccta cggtctcggg cacgagcccg accctgcgct cgccctcggc cacctcccgc
     7081 ggtattccaa ctttcacgcg ccagattata gcgtcggagc aggatctgcg ctcccgaagg
     7141 ccaaccgtta ccaacaaaac cccgttttgt aatcgaaatg tcacatatgg ggcttaaagt
     7201 tctactatcg accatagtat ctaatcgaga aaggaggtcg aggacgatga agaaacagag
     7261 tgccgcgtac cagaggagac tggccgcgct cgtggtggcc gtattcgcgt tcgccgagct
     7321 tggcctcctc gggtattccc tggtcgccgg aggttctccg gcctcggccg agagccccga
     7381 ccctgtacac ggcaggctct ctgaagagct cgagaacaag ttccacagga gtatcccgga
     7441 gaggattgtc gagacggtac ggggccccca ggcgaagacg ccggagagca agcggctcga
     7501 actcaccatc cctgcgctgg atcgagtgga cggcgtgccc gtctacgacg cctccgagag
     7561 cgggtacgag aaggctctcc acgacggtat cgtccacgtg aggggtacgg gctttccgtg
     7621 gcacaatgtg gccaacgtct acatcgcggg ccacagggtt ggttatccgg ggacgaagag
     7681 caacctcgtg ttctgggacc tcgacaagct ggagaagggg gatgaggtct tcctcacgga
     7741 cgcagacggg acccgctaca cctacgaggt cttcgagaag cgtgtggtct ctcccgacac
     7801 cgtcagcatc atgcggccta taaagggcag gaacatcgtt agcctccaga cctgtaccct
     7861 ccccgactac tcgcaccgcc tcatagtgca gggcgaactg aagggcagga actaaagacc
     7921 ctacctgggc gaatctgggc tgtgccttca accgttaccg gcacggccct attttgtaac
     7981 ctggatgtta ctttcgcgat gtatatctac acggcggtaa gagactcgaa tttgaggaga
     8041 ggaggtaggc tgacgtgagg acctacagga cgagaagaag cgggctcagg atgtttg
//
LOCUS       DATMJA010000221         2929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_681238, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000221 DATMJA010000000
VERSION     DATMJA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681238"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="tsaD"
                     /locus_tag="VNT12_05230"
     CDS             <1..828
                     /gene="tsaD"
                     /locus_tag="VNT12_05230"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWI45556.1"
                     /translation="DGVVEKALFDAEVTLDEVSSVAVTVRPGLIGALLVGVAAAKGLA
                     YARALPLVPVNHLEGHVAAAYLTDPDLQPPFVALIASGGHSALYSVDRDRKMRLLGET
                     LDDAAGEALDKGARMLGLGFPGGPAISKAARDGDRDRYEFPVGLKSKDNLDFSFSGLK
                     TSLLYKLKAMDGEQLREELPHLAAGYEAAVVEALSRKLLRAVDLQDAPTVVVAGGVAA
                     NAILRRTLEERCAKIGTHLIVPPPELCTDNAAMIGAAAPHSTAIAYPEYASLNASSV"
     gene            complement(869..1138)
                     /locus_tag="VNT12_05235"
     CDS             complement(869..1138)
                     /locus_tag="VNT12_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45557.1"
                     /translation="MSILSIFRTDRIPSLSRLPKELGREERPCARCGGRTEHILYRVP
                     KKVVLLYVKDHSENLHATCVVCARSTVLTGVERDRILARDSQTPE"
     gene            1245..2867
                     /gene="groL"
                     /locus_tag="VNT12_05240"
     CDS             1245..2867
                     /gene="groL"
                     /locus_tag="VNT12_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563800.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWI45558.1"
                     /translation="MAHKELRFNTEARRALESGVNKLAGAVKITLGPKGQYVVLDKKF
                     GSPTITNDGVTIAREIELEDIFENQGAQLVKEVATKTNDVAGDGTTTATVLAQIIVRE
                     GLKNVAAGANPVILRSGVEKAVEAAVEAIKAQSTEISGKEEISRVGAISARSDEVGNV
                     IADAIDKVGKDGVVNVEESQTFGMDLEFTEGMQFDKGYLSPYFVTDQDRMEAVLDDPY
                     ILIANQKIGNVQDVLPLLNQVMQSSKPLLIVSEDVEGEALATLIVNKLRGTFNAAAVK
                     APGFGDRRKRMLEDIAILTGGEVITEELGLKLENTQLNQLGRARKVVITKDDTTIVDG
                     AGETERITGRINQIRSEIETTDSDFDREKLQERLAKLAGGVAVIKVGAATETELKEKK
                     HRVEDALSATRAALEEGIVPGGGVVFLHAQQPVADLLDSLDGDERTGARIIYRALEEP
                     IRQIAQNAGADGSIVVDKVRTEGGNIGFNALSSEYEDLVSAGVTDPTMVSRSALQNAA
                     SIGSLIVTTDVIVAEPEEEAPAMPAGGMGGMM"
BASE COUNT          600 a          889 c          937 g          503 t
ORIGIN      
        1 gatggggtag tggagaaggc actgtttgac gccgaggtca ctctcgatga agttagcagc
       61 gtcgccgtga cggtacggcc gggcttgatc ggggccctcc tcgtcggggt cgcggcggcg
      121 aagggactgg cctacgcccg cgccctccct ctcgtccccg tgaaccacct ggaaggccac
      181 gtcgccgcag cgtaccttac ggaccccgac ctgcagccgc cgttcgtcgc cctgatcgcc
      241 tcaggcgggc attcggctct gtactctgtg gatcgggatc gcaaaatgcg cctgctcgga
      301 gagacgctcg acgacgccgc aggcgaggcc ctggacaagg gcgccaggat gctgggcctc
      361 ggtttccccg gcggacctgc gatctctaaa gccgcccgcg acggagaccg ggaccgttac
      421 gagttccccg taggcctgaa gagcaaagac aacctcgact tcagcttctc cggtctcaag
      481 acgagcctgt tgtacaagct taaggctatg gacggggaac aactccgcga agagttgccg
      541 cacctggctg ccggttacga ggctgccgtc gtcgaggccc tatcccgcaa gctccttcgg
      601 gccgtggatc tccaagacgc gcccaccgtc gtcgtagcag gcggggtcgc cgccaacgcc
      661 atcctgcgcc gtaccctcga agaaagatgc gcgaagatcg gaacccacct catcgtccct
      721 ccaccggagc tatgcaccga taacgcagcc atgatcgggg ccgcagctcc acactcgacc
      781 gccatcgcct acccggaata cgcgagtctg aacgcaagta gcgtttgaaa gaggtataag
      841 gcttcaggta tcaggacttg cagcgggtct actcaggcgt ctgcgagtcg cgggcgagga
      901 ttcggtcgcg ttcgacgccg gtgagaacgg tactcctcgc gcataccacg caggtcgcgt
      961 gcagattctc cgaatgatcc ttgacgtaga gcagaactac cttcttgggg actctgtaga
     1021 ggatgtgctc cgtgcgcccg ccgcagcgcg cgcaaggtcg ttcttcacgg cctagctcct
     1081 tgggcagacg gctcaggctc ggtatcctgt cggtacgaaa gatgcttagg atgctcatgt
     1141 ttgaattgta accccgccag gcatatactc tctacattgg cactctcatc gagagagtgc
     1201 caggcagttg aaatcgagtc ctagtttagc agaggaggca gatagtggcg cacaaagagc
     1261 taaggttcaa taccgaggca cgcagggctc tggagtcggg tgtaaacaag ctcgctggcg
     1321 cggtcaagat cacccttggt cccaagggcc agtacgtggt gctagacaag aagttcggtt
     1381 cgcccaccat caccaatgat ggtgtgacca tagcccgcga gatcgagctg gaggacatct
     1441 tcgagaacca gggggctcag ctggtcaagg aggtcgcaac caagaccaac gacgtggctg
     1501 gcgacggtac cactacggca acagttcttg cccagatcat cgtaagagag ggtttgaaga
     1561 acgttgctgc tggggccaat cccgtcatct tgagaagcgg tgttgagaag gctgttgagg
     1621 cggctgttga ggcgataaag gcccagtcca ccgagatctc gggcaaggaa gagatctcgc
     1681 gcgtaggggc catctcggcg cgttctgatg aggttggcaa cgtcatagcc gacgccatag
     1741 acaaggtggg caaggacggg gttgtgaacg tcgaggagtc gcagaccttc gggatggatt
     1801 tggagttcac cgaggggatg cagttcgaca agggctacct gagcccctac ttcgtcaccg
     1861 accaggatcg gatggaggcg gtgctcgacg atccctacat cctgatcgcc aaccagaaga
     1921 ttggcaacgt ccaggacgtc ctgcctctcc tcaaccaggt catgcagtcc agcaagccgc
     1981 tcctcatcgt ctccgaggac gtggagggcg aggctctggc tacgttgatc gtcaacaagc
     2041 tcaggggcac gttcaacgcg gctgcggtga aggcccccgg ctttggagac aggcgcaaga
     2101 ggatgctgga ggacatcgcc atccttacag gcggggaggt cataacagag gagctcggtc
     2161 tcaagctgga gaacacccag ctcaaccagc tcgggcgtgc ccgcaaggtg gttatcacca
     2221 aggatgacac caccatcgtc gacggtgccg gcgagacgga gaggatcacg ggtaggatca
     2281 accagatacg ctccgagatc gagaccaccg actccgactt cgaccgggag aagctccagg
     2341 agcggcttgc caagctggcc ggcggcgtgg ccgtcatcaa ggttggcgcc gcgaccgaga
     2401 cggagctcaa ggagaagaag caccgcgtgg aggacgcgct ctctgccacc cgcgcggccc
     2461 tcgaggaggg catcgtgcct ggcggtggcg tcgtgttctt acacgcccag cagcccgtcg
     2521 ccgacctgct cgactccctc gacggtgacg agcggacggg tgccaggata atctacaggg
     2581 cgctcgagga gcccatccgc cagatcgccc agaacgccgg ggccgacggc tccatcgttg
     2641 tcgacaaggt ccgcaccgag ggcggcaaca tcggcttcaa cgccctgagc agcgagtacg
     2701 aggacctcgt ctcggccggt gtaaccgacc cgacgatggt ctcgcgctcg gcgctgcaga
     2761 acgcggcctc catcggcagc ctcatcgtga ccacggatgt catcgtggcc gagccagagg
     2821 aggaggcgcc cgccatgccc gccggtggaa tgggcggcat gatgtaagga gctctccaga
     2881 agagcttcta ccggcggcgt cccgaaaggg gcgccgcttt ttcgtaacc
//
LOCUS       DATMJA010000222         3155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_29776, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000222 DATMJA010000000
VERSION     DATMJA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29776"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..1327
                     /locus_tag="VNT12_05245"
     CDS             461..1327
                     /locus_tag="VNT12_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI45559.1"
                     /translation="MGADDSTWGIAAQRWEELRKFRATHQPKHVTVKGVNWDYIAIGE
                     GGEGLLLLPGGAMVGESLFTRIPAFEDRYRVIAPDYPYVSTAALLLDGLAGVLDAEGV
                     RVAHVLGPSYGGLVAQCFVRRHPERVRSLILTNTAVPPQRLLWPARIFLTLLPFVPLG
                     WLQAHRERTLARAFSGVPSVPPEDQMFWRDYQHELVSRLSKEELRGMGRLGIDFVESF
                     RFAPDELASWPGRVLILESDEDIVTPEQRAELRRRYPQASVHTFHGAGHTPWMSHKEE
                     YLSVINEFLDQE"
     gene            1340..>3155
                     /locus_tag="VNT12_05250"
     CDS             1340..>3155
                     /locus_tag="VNT12_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWI45560.1"
                     /translation="MTYTTWFDEIRKDDIALAGGKGANLGELSRAELPVPPGFVVTTT
                     AYDAFVEANGIADAIVGRALVTRVDDPDAFEEVAEGIRALFSGGKVPEEMADEIRAAY
                     HQLSEDAVERPVAVRSSATAEDLPGMSFAGQQETYLNVRGAEALLEGVKNCWASLWTA
                     RAMAYRARQGVDPATVSLAVVVQRMVKSEAAGVMFTANPSNGRRDQATISAAWGLGES
                     VVSGSVTPDSIVVEKGSGRVLSREISDKDVMTVYSENGTEQRPVPEARRRQPVLDDGA
                     AAELERYGKRIEDHYGTPQDIEWALVGGEFFIVQSRPITALPEPMTDPPTNWSVPDPK
                     GFYVRASIVEQMPDPLSPLFADLAYGSVPRSLDKLMNELLGSDTLREGELAFPTVNGY
                     AYYYYKMAAILRISAKALAVLPSLYGSGKASGVKRWREYAQPRYATIVKEWEAKPPED
                     LPAVGLLAGARELLDAGTEYYTSVQTIIPIAATSELLFTGFYDRLIRREGDPPGQTFL
                     LGFDSMPIRAEKSLYDLAMWCGEHAELAAALLDTPSERALELLRMEVPPAGMGERVWD
                     DWRLRFQIHLDRFGHAVYNLDFVNPVPADDPTPLFDTLK"
BASE COUNT          600 a          933 c         1049 g          573 t
ORIGIN      
        1 tcgaagatag ggttaggctc ccctgtgcga atgcacaggg agcagagagc ctggtagctc
       61 gttggacttt aaagccgaat accataactg ctcgctctcg ttgacacctt gcagcatggc
      121 gctgtctcca tgccgacagc aatgacgcag ctgcgctgat cgtaggcgag ttcgtgggga
      181 gaggcagctc accgggtacg gtcggaggtc tggagcttga gcgacgaacc gctgacggag
      241 cttgttagac tttccaggtc gctgggaaga cccgaacgag agctggtggt gctcggggaa
      301 ggcaatacct cggccctcat ggatggcgag gcgctcccca ggttttcgtg tacgcaacct
      361 cagcccgaga tatattaata gtcgtagcag tttagtagtt ctcaagatag taggtagtca
      421 acttggctat gcggctaggg catggagtag gatcctggtg atgggagccg atgattctac
      481 ttggggcatc gcagcccagc gttgggagga gctacgaaag ttccgggcca cacaccaacc
      541 aaagcacgtc accgtcaaag gggtgaactg ggactacatc gccatcggcg agggtggcga
      601 ggggctcctg ctcttgccgg gtggggcgat ggtcggcgag tccctcttca cgcgcatccc
      661 ggccttcgag gatcgctacc gggtcatcgc gccggattat ccttacgtgt ctaccgcggc
      721 gcttctcctc gacggtctgg cgggcgtcct ggatgccgag ggggtgcggg tcgcgcacgt
      781 gctcgggcct tcttacggcg ggctcgtggc gcagtgcttc gtgcgacgcc atcccgagcg
      841 ggtgcgatcg ctgatactga cgaacaccgc agtgccgccg caacggttgc tgtggccggc
      901 gaggatattt ctgacgctgt tgcctttcgt cccgctcgga tggctgcagg cgcataggga
      961 gcgcacgctc gcgcgggcct tctctggtgt gcccagcgtg ccaccggagg accagatgtt
     1021 ctggcgcgac taccagcacg agctcgtgtc gcgcctctcg aaagaggaac ttcgcggcat
     1081 gggccgactc gggatcgact tcgtggagag cttccggttc gcgccggacg aactagcctc
     1141 ctggccggga cgggtcttga tcctggagtc ggacgaggac atcgtgacgc cggagcagcg
     1201 cgccgagctc aggcggcgct acccgcaggc cagcgtgcac acgttccatg gcgccggaca
     1261 cactccctgg atgagccaca aggaagagta cctttccgtc ataaacgaat tcctcgacca
     1321 agagtaaggg gattggtaga tgacctacac aacatggttc gatgagatac gcaaagacga
     1381 catcgcgcta gcaggtggca agggtgccaa cctgggcgaa ttgagccgcg cggagcttcc
     1441 cgtaccacct ggcttcgtcg taaccaccac agcctacgac gccttcgtcg aggcaaacgg
     1501 tatcgcagac gcgatcgtcg ggcgcgcctt ggtgacccgg gtggacgacc cggacgcctt
     1561 cgaggaggtg gcggagggga tccgcgcgct tttctccggc ggcaaggttc ctgaggagat
     1621 ggctgacgag atacgcgccg cctaccacca gttgagcgaa gacgcagtag agaggccagt
     1681 cgccgtccgt tcctcggcca ccgcagaaga cctgcctggt atgagcttcg ctggccagca
     1741 ggagacctat ctgaacgttc gcggagcgga ggcgctgctc gagggggtca agaattgttg
     1801 ggcttctctg tggacggcgc gggcgatggc ttaccgggca cgccagggcg tagatccggc
     1861 aaccgtgagc ctggcggtgg tggtccagag gatggtcaag tcggaggccg ctggagtgat
     1921 gttcacagcc aacccctcga atggtcgccg ggatcaggcg acgataagcg ccgcgtgggg
     1981 acttggggaa tcggtggtaa gcggctcggt gacgccggat tcaattgtcg tggagaaggg
     2041 gagcgggcgt gtgctatctc gcgagatctc cgacaaagat gtcatgaccg tctactctga
     2101 aaacgggacc gagcaaagac ccgtgccgga agcgcggcgc cgccagccgg tgctcgatga
     2161 tggggcagcg gcggagctgg agcgttacgg taagcggatc gaggaccatt acggaacccc
     2221 gcaggacatc gagtgggcgc tcgtgggcgg cgagttcttc atcgtgcagt cccgtccgat
     2281 aaccgccctg cccgaaccga tgaccgatcc tcccacgaac tggagcgtgc cggacccgaa
     2341 gggcttctac gttcgcgcca gcatcgtcga gcagatgcca gacccgctct cgccgttgtt
     2401 cgccgatctt gcctacggat cggtcccccg ctccctcgac aagctcatga acgagctcct
     2461 cggttccgac actctcagag agggcgagct cgcattcccc acagtcaatg ggtacgccta
     2521 ctactactac aagatggcgg ccatcctgag gatcagcgcc aaggctctcg cggtccttcc
     2581 gtccctgtac ggaagtggaa aggcaagcgg cgtaaaacgc tggcgcgagt acgctcagcc
     2641 gcgctacgcg acgattgtga aagagtggga agccaagccg ccagaagatc tgccggcggt
     2701 cggactcctc gcgggcgcgc gggagctact ggacgcaggg acggagtact acacctcggt
     2761 gcagaccatc atcccgatcg cggccaccag cgagctgttg ttcaccggat tttacgatcg
     2821 gctcatcagg cgagaggggg atcctccggg acagaccttc ctgttgggtt tcgacagcat
     2881 gcccatccgg gccgagaaat cactctacga cctggcgatg tggtgcggcg agcatgccga
     2941 gctcgccgcg gcgctcctgg atacaccgtc ggagagggcg ctggaactgc tgcgcatgga
     3001 agtaccaccc gccggtatgg gcgaaagggt gtgggacgat tggcggttgc gattccagat
     3061 tcacctggac cgcttcgggc acgccgttta caacctggac ttcgtgaacc ccgtgccggc
     3121 tgacgacccc acgcccctct tcgacacgct caaat
//
LOCUS       DATMJA010000223         3777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_741318, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000223 DATMJA010000000
VERSION     DATMJA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741318"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VNT12_05255"
     CDS             <1..729
                     /locus_tag="VNT12_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI45561.1"
                     /translation="ATIERTFVSTVHPSSVAAIIVEPVSGEGGFIPFPDFYLRRLREL
                     CDEHGIVLIVDEVQTGFGRTGKMFAIEHSGVEPDIITTAKSMGGGLPIAGVTGKAEIM
                     DSVHVGGLGTTYGGNPIACAAALAVLETYEEDDLVARANALGDRVMGAMREIQERHPD
                     FVGDVRGRGPMAAMEFVKDAASKTPDKERTQRVIETSLQQGLLLLSAGQYGNCIRTLM
                     PFVITDEELEEGLAVLGRAVDASA"
     gene            complement(731..1294)
                     /locus_tag="VNT12_05260"
     CDS             complement(731..1294)
                     /locus_tag="VNT12_05260"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWI45562.1"
                     /translation="METSRQQLTRWRFTVHDYHRMGEAGILHEDDRVELIEGELVEMT
                     AIGTRHFSCVNRLNRLLVVNVGDEAIVSVQNPVRLNEYTEPQPDLTVIRPRDYSLSLP
                     GPEDVLLLIEVSDTTLAYDRGVKLPLYARAGIREVWIVDLTRETVGRHTDPSGEVYRR
                     AERLRRGQTLESISLPGLTPSVDEVLG"
     gene            complement(1536..2042)
                     /locus_tag="VNT12_05265"
     CDS             complement(1536..2042)
                     /locus_tag="VNT12_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HWI45563.1"
                     /translation="MVKGDQYQPAVAGVTVSFIERLALKASTITRVALMAAVTAVAAQ
                     ITIPLFPVPFTLQVLAVILSGLLLGVRHGALAQAVYVLVGAVGVPVFAGFTGGLGHIF
                     GPTGGYLISYPIAAAVAGIAAKTVAGDSRTRRYGLASSGDARASRLSTPSEQPGSPWS
                     QASHPQRP"
     gene            2145..2333
                     /locus_tag="VNT12_05270"
     CDS             2145..2333
                     /locus_tag="VNT12_05270"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWI45564.1"
                     /translation="MGRRSTSGREDKDEGAMDRAKGKMKEAAGALTGEKDTKAEGRAD
                     QRRGRMKEKKGNLKDLLK"
     gene            complement(2454..2954)
                     /locus_tag="VNT12_05275"
     CDS             complement(2454..2954)
                     /locus_tag="VNT12_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45565.1"
                     /translation="MATTKRINRSAERLAKTSADSYKMVIDHVIARQERNVKFAQEMF
                     GGTVREIRHQAESNRALTRELVERAEIQRDAFRTLVGESVDAYTDLLYAPLAYYKQGL
                     RLVESEGAGFPIAGYDELNVKEIGDHLDGMTAAEIRRVREYEKRNKNRETLIEQFDRK
                     LKAASA"
     gene            3266..>3777
                     /locus_tag="VNT12_05280"
     CDS             3266..>3777
                     /locus_tag="VNT12_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWI45566.1"
                     /translation="MRLRNVVLILAALTVVLMLAACSSSGQASDEGGKDQAGKGEQAQ
                     KQKPPEKATKGKKEPPKMTKEAKDKQIAPKKGKAQGQRKVANSLEPPKDKTLKLTIPA
                     IKQIEDDTIPTGKGTNETLFHDHAAVHLRDTGFPWQRTANVYIAGHRIGFPGTDSNLA
                     FYDLEDLEKG"
BASE COUNT          774 a         1139 c         1179 g          685 t
ORIGIN      
        1 gccaccatcg agcggacgtt cgtgagcacg gtgcatccta gcagcgtagc cgcgataatc
       61 gtcgagccgg tgagcggtga aggcggtttc ataccgttcc ccgattttta tctgcgaagg
      121 ttgcgcgagc tgtgcgacga gcacggcatc gtcctgatcg tggacgaggt ccaaaccggt
      181 ttcggcagga cgggcaagat gttcgccatc gagcactccg gggtcgagcc cgacatcatt
      241 acgacggcca agagcatggg tgggggactg cccatagccg gcgtcacggg gaaggcggag
      301 atcatggact ccgtccacgt cggcggcctc ggcaccacct acggcggcaa cccgatagcc
      361 tgcgccgccg ccctcgccgt gctcgagacc tacgaagagg acgacctcgt cgcgcgcgcc
      421 aacgctctgg gcgaccgcgt catgggcgcg atgcgcgaga tccaggagcg ccaccccgac
      481 ttcgtcggcg acgtgcgcgg caggggcccg atggccgcga tggagttcgt caaggacgcc
      541 gcgagcaaga cccccgacaa ggagcggacc cagagggtga tcgaaacctc cctccagcag
      601 ggactcctgc tcctcagcgc cggccagtac ggcaactgca tccgcaccct catgcccttc
      661 gtcatcaccg acgaggaact ggaggaggga ctggccgttc tcggacgcgc cgtagacgcc
      721 tcagcctgaa tcagccgagg acttcgtcca cgctcggggt caggccgggt agcgagatgg
      781 actctagcgt ctgcccacga cgcagccgtt ctgcgcgtcg gtagacttcc cctgacgggt
      841 cggtgtgacg acctacagtc tcccgtgtaa ggtcaacgat ccagacctcc ctgattcccg
      901 ccctcgcgta gagcggcagc tttaccccgc ggtcgtaggc gagggtggta tcggagacct
      961 cgatcaggag cagcacgtct tctgggccag ggagcgagag gctgtagtcg cgcgggcgga
     1021 tcaccgtgag gtcgggctgg ggctcggtat actcgttaag ccttacgggg ttctggacgc
     1081 tgacgatggc ctcgtcgccc acgttgacca ccagcaaccg gtttagccgg ttgacgcacg
     1141 agaaatgcct ggtaccaatt gctgtcatct cgactagctc gccctcgatg agctcgaccc
     1201 gatcgtcctc gtgcaggatc cccgcctcgc ccatgcgatg gtagtcgtga acagtaaagc
     1261 gccagcgggt aagctgttgc ctgctcgtct ccacgggaac gacctcctgt ggttcgcccg
     1321 tctcaagaag aagtatactc ggatcagaag acgaaccttt tatctactcg cgccaccctg
     1381 ccctggttcc tctatgtaat ccacggggcg ccgccggtgc tttgggctcc ctcaggttcg
     1441 cgaaggaacg atagcaggcg ctgccgcgac cgcgacgaac gccgccaacc ccacttttat
     1501 caggtcgaag atgacgaagc gcccgacgcc cgcaactatg gcctctgtgg gtgtgaggcc
     1561 tgagaccacg gcgagccagg ttgctccgaa ggcgtagata accgcgaggc ccgcgcatcc
     1621 ccagaagaag ccaaaccata gcgccttgtg cgggaatctc ccgcgacggt cttcgcggcg
     1681 atgcctgcca ccgctgcggc tatggggtag gagatcaggt atcctcccgt aggcccgaag
     1741 atgtggccga ggccgccagt gaaaccggcg aagaccggca cgccgacggc tccgacgagg
     1801 acgtagaccg cctgcgcgag cgccccgtgg cgcactccga gcaggagccc ggagaggatg
     1861 acggcaagta cctgcagggt aaaaggaacg gggaagagcg gaatggtgat ctgggccgcc
     1921 acggccgtca ccgccgccat caacgccaca cgggttatgg tggatgcctt caacgccagc
     1981 ctttctatga aagagacggt cacccccgcg accgccggtt ggtactgatc cccttttacc
     2041 accgttcgtc cccggtttcc ggcagcaaag gtgccagcag gcgtttgatt ccagcacctc
     2101 cgggtaagta aactaatgaa cattcccggg gcgaggagga acaggtgggt agacgaagta
     2161 cgagcggccg ggaggacaaa gacgaaggcg ccatggacag ggccaagggt aagatgaagg
     2221 aagcggccgg cgccctgacc ggtgagaaag acacgaaagc cgagggccgc gcggaccaac
     2281 gccgcggaag gatgaaggag aagaagggta acctgaagga cctgttgaag taaggcttca
     2341 ggtaaagaag gtcaatcaga caggcgaaag gccggggatt ctctccccgg ccttctctct
     2401 tatcagccgc gttggtctgc tttagaacct gacgactgaa acctgatgcc tgcttacgcg
     2461 gaagcggcct tgagcttgcg gtcgaactgc tcgatcaggg tctcacggtt cttgttccgc
     2521 ttctcgtact cgcggaccct gcggatctcc gctgcggtca tgccgtcaag atggtcaccg
     2581 atctccttga cgttcaactc gtcgtagccg gcgatcggga accctgcacc ctcgctctcg
     2641 acgaggcgca gaccctgctt gtagtaggca aggggcgcgt atagcaaatc cgtgtaagcg
     2701 tcaaccgact cgccaaccag cgtccggaaa gcgtcgcgct gtatctcggc ccgctcgacg
     2761 agctccctgg tgagcgccct gttgctctca gcctggtgcc tgatctcacg caccgtgccg
     2821 ccaaacatct cctgtgcgaa cttcacattg cgctcctgcc gcgctatcac atggtctatg
     2881 accatcttgt aggagtcggc gctcgtcttc gccagccgct ccgcactcct gttgatccgc
     2941 tttgtagtag ccactctctg cctcctctta atggacatca tctatgtgca gtgtacacat
     3001 atattcaggt atgtcaaggt tgatatctgt cgcaggcaag aatcggttgt gcccatggct
     3061 ctagatcctg gcctttccac ctgggattgc gaaccttaat atgaccgtcc tgatccaggc
     3121 gtggctgaca tcgtgggcgt ggattgagag gtctcttgcg gtgaggtttg taacggatgt
     3181 ttggtctaac tgttatgacc ttgttatctt ggatgtgtat gctctctgag cacggcgtct
     3241 ggtggagaca gcgagaggtg atgagatgcg tttgagaaat gtggtgctga tattagcggc
     3301 actgacggtg gttctgatgc tggccgcttg ctcttcctcg ggccaagcct ccgatgaggg
     3361 ggggaaggat caggcgggga aaggtgagca ggcacagaag cagaaacctc ccgagaaggc
     3421 gaccaagggc aagaaggagc ctcccaagat gacgaaggag gcgaaggaca agcagatcgc
     3481 gccgaagaag ggtaaggcac aggggcagcg taaggtagct aactcgctcg agccccccaa
     3541 ggacaagacc ctcaagctca cgattcccgc gataaagcag atcgaggacg acacgatacc
     3601 gacaggcaaa ggcaccaacg agacgctgtt ccacgaccac gctgctgtcc atctgaggga
     3661 cacgggtttc ccgtggcaga ggactgccaa cgtctacatc gcgggacacc gcatcgggtt
     3721 ccctgggact gacagcaacc tggctttcta cgatctcgag gacctggaaa agggcga
//
LOCUS       DATMJA010000224         6023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_681600, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000224 DATMJA010000000
VERSION     DATMJA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681600"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VNT12_05285"
     CDS             <1..212
                     /locus_tag="VNT12_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HWI45567.1"
                     /translation="AYTDPSWTPLFVAIKGLVTEVGGLMTHGAVIAREYGLPAVVGVE
                     HASRLIRDGQRIRVHGTDGYVEILP"
     gene            complement(265..468)
                     /locus_tag="VNT12_05290"
     CDS             complement(265..468)
                     /locus_tag="VNT12_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45568.1"
                     /translation="MDEEAKWRSMEQVRAHLARADSELEAPQRLLDPRTDDELEIAMV
                     RAVANARTLVGDAPETIGARIWE"
     gene            complement(552..722)
                     /locus_tag="VNT12_05295"
     CDS             complement(552..722)
                     /locus_tag="VNT12_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45569.1"
                     /translation="MCYTRKDRDLKREARWITEKRLMEEEARHRQEEHDRRREQEKKP
                     LTEKIKEVVGVR"
     gene            1311..2444
                     /locus_tag="VNT12_05300"
     CDS             1311..2444
                     /locus_tag="VNT12_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HWI45570.1"
                     /translation="MRTQTVTTDEDQRVTFIELFFDLVFVYAVTQLVALLHHELSWPG
                     AGRAVLVFWLVWWAWTQFTWALNSADTTHPGVELSVLVATAVAFLMAVALPDAFGGSG
                     AWFALTYVSVRVLGLAVYGRVAWEDPAKRAAVRRFALLSVGGLVAVAAGGVAPADARV
                     WWWAAAVGFDLFTAGVAGTAEGWDIRPDHFGERHGLFVIIALGESLIVAAAGLSGAER
                     TAHTVAVGILAVAGTCGLWWTYFGWAKPAIDHAVAERADPARTILARDVYSVLHFPVV
                     FGIVLYAVAIEAAVAHPDAPLSVGSGIALDGGITLYLGGTAAALWRAGHRVLLPRTVI
                     VGAVLASLWAARDAAATWSLVIAVCGVAAVAAVEQLAQLRKAR"
     gene            2452..3024
                     /locus_tag="VNT12_05305"
     CDS             2452..3024
                     /locus_tag="VNT12_05305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HWI45571.1"
                     /translation="MSEKDFKILGLAASLRRASIHRGILRAAHEVCPEGMACDSFDLG
                     RIPFFNADVEAKGDPEAVRELKEKIRTYNAVLIATPEYDYAIPGVLTTALDWCLRRSS
                     PFRHKPVGIAGASPSGAGTVLGQMVLRQILLHGPAYVMPEPRMLIPNAYQRFDENGDL
                     TDRETRQRLRRFLEALREWNASLDRAGLLE"
     gene            complement(3696..4019)
                     /locus_tag="VNT12_05310"
     CDS             complement(3696..4019)
                     /locus_tag="VNT12_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWI45572.1"
                     /translation="MEASSWRFACLRALRLPLRLPLPCHSIRPCLCPGRRLRGSLSYT
                     FSREVSEAAKKNRRVLLEAMVGAGFFNYGYEWWHYSYGERYWAHAGGERAARYGSLWR
                     SRRGP"
     gene            4174..4596
                     /locus_tag="VNT12_05315"
     CDS             4174..4596
                     /locus_tag="VNT12_05315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWI45573.1"
                     /translation="MAAEPGATVNLEGESAFMRVLGLNFQEMGPERVVASFEAGPDHH
                     QPWGLVHGGVFTAVIETVATTGAYHAVKDEGRLAVGVDNVTDFLRPHQSGTLRAFAEP
                     LQQSKTQQLWQAVIEREDGKAVARGRVRLQNIEARRDG"
     gene            4589..5047
                     /locus_tag="VNT12_05320"
     CDS             4589..5047
                     /locus_tag="VNT12_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HWI45574.1"
                     /translation="MADSRTSLQESILGAWTLSSYVIADVSGENREYPLGDDASGLLI
                     YSADGFMSVQISRPGRPAYADGALHGGTDAERAPAAAGYLAYAGRYSVQGDVVTHKPL
                     ASLFPNWEGFDVPRRARITGETLTLDLLEPIHQNGRQRTGTLTWRRAASL"
     gene            complement(5526..5819)
                     /locus_tag="VNT12_05325"
     CDS             complement(5526..5819)
                     /locus_tag="VNT12_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45575.1"
                     /translation="MYAAVRRFEGVTDSQKVVQVAKEGFLPIISEMPGFVAYYLVDAG
                     DGVTVATSVFEHKDAEEQSTFVAGEFVAEHLAPLMPNPPQVTAGEVVAYKVSS"
BASE COUNT         1101 a         1953 c         1860 g         1109 t
ORIGIN      
        1 ccgcctacac ggaccccagc tggacgcccc tgttcgtcgc gatcaagggc ctggtgacgg
       61 aggtgggggg cctgatgacc catggcgcgg tgatcgcacg ggagtacggc ttgccggccg
      121 tcgtgggggt ggagcatgcc agccggctga tccgggatgg gcagcggatc cgcgtgcacg
      181 gaacggacgg ttacgtcgag atcctgccct agccgttttc gcagcgctcc ggctctgctt
      241 cttccagctt cgttagcctg acgctcactc ccagatccta gctccgatcg tctccggtgc
      301 gtcgccaacc aacgtcctgg cgttggccac agcacgcacc atggcgatct ccaactcgtc
      361 gtcagtccgt gggtcgagga gcctctgggg ggcctccagt tcgctgtcgg ccctggccag
      421 gtgcgccctc acctgctcca tggagcgcca cttggcttct tcgtccacga tcgcggtctc
      481 ccttcgagca tgaagagagg ccccggtatt cggggcctca tgctctcctt gctagttgtc
      541 ctccgtaggg actacctgac gcctactact tccttgatct tctcggtcag gggcttcttc
      601 tcttgctctc tcctacgatc atgctcctct tggcggtggc gggcttcctc ttccatcagc
      661 cgcttctcgg tgatccagcg tgcctctctt ttgaggtcac ggtctttgcg cgtgtagcac
      721 atcagcggat acctccttcc catagcgacg cttccgttgc tcattttaat agatatgtat
      781 ctcaagtgcc gggtaagatg atcatctagc aaggatctac tcggcggttc tcctaggcca
      841 gcatccctcg attccactgg actttgcaga ttatcctttc tcattttccc cgtgcgatag
      901 cgcgatgacc cagtctagac gcttctcagg gcagagagtc ccagagctct gccttttggt
      961 ttccctcggc caatcggagc tcatcttccc acaagggggc tcttcgcctg tgcagatccc
     1021 tggccatagt gtccacaacg ttgacgccat gaaggatggc cagtaggctc cgagtgaggc
     1081 gaaggacgag agggtgcgac ctgaggatcc acgtgcccgt ggacatggac atcggtgggt
     1141 agccggaggc cgtgtggtac gcgctcagcc gcccgtccgc agccccctcc accaggcagg
     1201 aaggccgtgg ccagcggcgg ctgagggagc cggttcgatc agacactttt gagaggaccg
     1261 cgcgaggcta agccgatcaa ccacgcgacc ccgtcggaaa gagacctgtg atgagaacac
     1321 aaaccgtcac gacggatgag gaccagcggg tcaccttcat cgagctgttc ttcgacctgg
     1381 tcttcgtcta cgccgtcacc caactggtgg ccttgctcca ccacgagttg agctggcctg
     1441 gcgccgggcg ggccgtcctg gtgttctggc tggtgtggtg ggcgtggaca cagttcacgt
     1501 gggcgttgaa ctcggccgac accacgcatc cgggggttga gctcagcgtg ctggttgcca
     1561 ccgcggtggc gttcctgatg gcggtggctc tccctgatgc ctttggcggc agtggcgcgt
     1621 ggttcgccct cacctacgtg agcgtgcgcg tattaggcct ggccgtatac ggccgagtcg
     1681 cctgggaaga tccggcgaag cgggccgcgg tgcgccggtt cgccctgctc tcggtcggtg
     1741 gcctagtagc ggttgccgct ggtggcgttg cacccgccga tgcccgggtg tggtggtggg
     1801 cggcggccgt cggattcgac ctatttaccg ccggggtagc cggcaccgca gagggttggg
     1861 acatccgccc cgatcacttc ggcgagcgcc acggcctctt cgtcatcatc gcactcggcg
     1921 agtccctcat cgtggctgcc gctggcctct ccggcgccga acgcaccgct cacacggtgg
     1981 ccgttggaat cctcgccgtg gccggcacgt gcgggctgtg gtggacctac ttcggctggg
     2041 ccaagccggc catcgaccat gcggtggccg aacgcgcgga tcccgcccgc acgatcctgg
     2101 cgcgcgacgt ctacagcgtc ctgcacttcc cggtagtgtt cgggatcgtg ctctacgccg
     2161 tggcgatcga ggcagccgtc gcgcacccgg atgcgccact ctcggtcggc tcgggcatcg
     2221 cgctggacgg tggcatcacc ctctatctcg gcggaaccgc agccgcgctg tggcgcgctg
     2281 gccaccgggt gctcctcccc cgcacggtga ttgtcggtgc cgtcctcgcc agtctttggg
     2341 cggcgcgtga cgctgccgcg acctggtcac tcgtgatagc tgtctgcggt gtagcggccg
     2401 tggcagcagt cgagcagctg gcacaactga ggaaagcgag gtagcacaga ggtgagtgag
     2461 aaggatttca agatactggg cttagcggcg agcctcaggc gtgcttcgat ccaccgcggc
     2521 atcctacgcg ccgcccacga ggtctgtccc gaaggcatgg cctgcgacag cttcgatctc
     2581 gggcgcatcc ccttcttcaa cgccgacgtg gaggctaagg gagacccgga ggcagtgcgc
     2641 gagctcaagg agaagatccg gacttacaac gccgttctca tagcgacccc cgagtacgac
     2701 tacgccatac ccggggtgct caccaccgcc ctagactggt gcctcaggcg ctcctcgccc
     2761 ttcaggcaca agccggtcgg catcgcaggc gcgagtccga gcggggccgg cacggtcctc
     2821 gggcagatgg tcttgaggca gatccttttg cacggcccgg cctacgtgat gcccgaaccc
     2881 cggatgctga tcccgaacgc ttaccagcgc ttcgacgaga acggagacct caccgacaga
     2941 gagacccgcc agaggctccg gcgctttctg gaggcactta gggagtggaa cgcgagtctg
     3001 gaccgcgctg gactcctcga gtaggaaaat gctgagtggc cagcacggtg acgagagaca
     3061 aacacgggga ggatcagtaa atgagagagc agttgtagtc ctccttcaaa gttagccaaa
     3121 atgttcttca aaccaatgtg gcgccactcc aagccccttg ctctacccga aactggttca
     3181 gcaccttctg gggcacgcca gcataacaat gactcttgat cgctactcgc agtggatagc
     3241 caacatggga aggcacgccg ccgagggtat ggacaaggcc ctgggttagc gacgccgctt
     3301 accggcgagt gcaaagccac tcaggaaacg tgcgtggtcc gatccaagtc taccgcggca
     3361 gtggagcatt cacgagcccg tatatcgatc caacaccaca atatataggg gtctgtcatc
     3421 gagtgtggat gcgctaagga tcgagccttg cggttttgag gtggtgttct atgcacgatg
     3481 ctgtatgcag atttcggaga attcctcttc cacgcacttc ggtgcataag ggtgaaatca
     3541 cgctatcggc ttttaggctt cgaacctcgc cataaaccct gcgacgcata ggcggacttg
     3601 ggatagcggg aaatcgggtc agcgaaacac acctggccac ggccgtggcc ggccgtggcc
     3661 gcacatgtcg gtcggggcga cggcagtacc gacgctcacg gcccccggcg cgagcgccac
     3721 agggagccgt agcgggccgc gcgttcgccc ccggcgtggg cccagtagcg ctcgccgtag
     3781 gagtagtgcc accactcgta gccgtagttg aagaaccccg cccccaccat agcttccagg
     3841 aggacgcgcc ggttcttctt ggccgcctcg gagacttccc gcgagaaggt gtacgagagc
     3901 gatcctcgca gtcgtctacc cgggcagagg caaggacgga tagaatggca cggtagcggc
     3961 aaacggagcg ggagacgtag tgcccgaaga caggcgaacc gccaagaaga cgcctccacg
     4021 cggccctaga tcgcctgtcc cgccgcagct tcgacgaggc cgaggctcct cgaatagagg
     4081 ggtcaacgtg atgcctgctg gcgtgggggc cgcgagcccg cccggcgcca taatcggagc
     4141 gacacgacgc cgaagaggcg aaaggagaca gagatggctg ccgaacccgg cgcaacggtc
     4201 aacctcgagg gcgagagcgc cttcatgcgc gtcctgggcc tgaacttcca ggagatggga
     4261 cccgagcggg tcgtggcctc gttcgaagcc ggtcccgacc accaccagcc gtggggactg
     4321 gtgcacggcg gggtattcac cgccgtgatc gagacggtgg cgacgaccgg cgcctaccac
     4381 gcggtcaagg acgaaggccg tctcgcggtc ggcgtcgaca acgtcaccga cttcttgcgc
     4441 ccccaccaaa gcggcacctt gcgcgccttt gccgagcccc tgcagcagag caagacccag
     4501 cagctctggc aggccgtgat cgagcgtgag gatggcaagg ccgtcgcgcg cggccgggtc
     4561 cgcctgcaga acatcgaggc ccgccgagat ggctgactcg cgaacctccc tgcaggagtc
     4621 gatcctcggc gcgtggacgc tcagttcgta tgtgatcgcc gacgtcagcg gcgagaaccg
     4681 cgagtaccca ctgggcgacg acgcgagcgg cctgctgatc tacagcgccg atggattcat
     4741 gtcggtgcag atcagcaggc cgggacgccc ggcatacgcg gacggggccc tgcacggtgg
     4801 caccgacgcg gagcgagcgc ccgcagcggc ggggtacctg gcctacgccg gccgctacag
     4861 cgtccaaggc gatgttgtca cgcacaagcc gctcgccagc ctgttcccga actgggaagg
     4921 cttcgacgtc cctcgccgag cgcggatcac cggcgagact cttacgcttg atctgctcga
     4981 accgatccac caaaacgggc gccaacgcac gggtaccctc acctggcgcc gtgccgcttc
     5041 gctctaaccg ataagactcg ccgagcccaa tggcggctgg tgacacgttc cttaccgaca
     5101 ccccagatcc cgcgaccccc aaaagaaatc cccgctcgta aacccctccc aggccagaaa
     5161 cacgcaaaag agcagtaacc acgggaggca ccctaagcga actttcacgc cctacacctt
     5221 ccctatatca tcaacaggac cgttagcttt ctcctcaaca ccacttgcac actcctccca
     5281 taaggtagga cggaactctt cagctccgcc tttgttggtt acgagggcaa gtatgcatgg
     5341 acacaagggg cgagccatct cccaggtggc catttcttct tggcgcgaac ttccaagaac
     5401 tccgttatgg cgaagttcgc agaattcccc ttccacgcac ttaggtgaat aagagaaagg
     5461 ggaagggccg gggctgctac gccccggctc tctcacatct agctctcgaa ctctccgtct
     5521 ctcgcctacg acgacacctt gtatgccaca acctcgccgg ctgtgacttg gggtgggttc
     5581 ggcatcaagg gagccagatg ctccgctacg aactctcctg ccacaaaggt ggactgctcc
     5641 tcagcgtcct tatgctcgaa gacgctggtc gcaactgtta ccccgtcgcc agcgtcgacc
     5701 aagtagtaag ccacaaatcc cggcatctcg ctgatgatgg gcaaaaagcc ttccttggct
     5761 acttgcacca ccttttgcga gtcggtcacg ccctcgaagc gccgcactgc agcgtacata
     5821 gctttcctct acctccttaa gagagggcga gacccttagg ccccgccttg agttgatcaa
     5881 cgaaggtaac tatgaggggt tacaaggcct ggcgcaactt cggagaatcc ccattcggcg
     5941 aacttcgcct taacggcgtt ctgagaaatt cagccgaacg gggcgaacgg agcgccgagt
     6001 agggcaccta tgccgaccgc gac
//
LOCUS       DATMJA010000225         6012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_859138, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000225 DATMJA010000000
VERSION     DATMJA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859138"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VNT12_05330"
     CDS             <1..578
                     /locus_tag="VNT12_05330"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWI45576.1"
                     /translation="LGRPLDPDPPLDEMIVRYLGAFGPATVKDVQTWSGLTRLGEVIE
                     RIRPRLRIFRDERGKELFDVPDAPMPDPDPPAPPRFLPEFDNLILSHADRTRVIGEEY
                     RKAIASKNGMVPATVLVDGFVRGTWKTERSRGKANLEIKPFEPLTKEDRDALAEEGER
                     LIRFTGAEAYEIRFAKPWKIHGTSEHSRIGH"
     gene            612..776
                     /locus_tag="VNT12_05335"
     CDS             612..776
                     /locus_tag="VNT12_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45577.1"
                     /translation="MIDTSTILIPKDRRSFERLMENLHRKEHETSATIAEAGGLAVRI
                     ILIPAPMDWL"
     gene            785..2419
                     /locus_tag="VNT12_05340"
     CDS             785..2419
                     /locus_tag="VNT12_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI45578.1"
                     /translation="MDATDELERGRESYVRREWMDAYESLSRADQAAPLGAEDLELLA
                     TSAYMLGRDDDMSGMERAYHVYLDAGEALRAARCAFWVGMHLFTRREMGRGTGWLGRA
                     QRLVEREGRECVEQGYLKLPLMFQQEAAGDYEAAAAIAAEAAEIGERFRDADLFALAV
                     QAQGIFLIKHGRVEEGLGLLDEAMVAVTAGELSPIVSGLVYCGMILGCQDAYELRRAQ
                     EWTAALTRWCKEQPDMVAFTGTCMVHRAEIMQLHGAWLDAIEEARRAGERSAQAMNRT
                     AVANARYLQGEVHRLRGEFAAAEEAYQDASRGGWEPQPGLALLRLAQGDSGAAGAAIR
                     RAVSEASEPSKRVGLLPAYVEIMLAIGDVQEARSACRELGEISAGFQSGMLGAMAAHA
                     RGAIDLADGDIRAALVALRHARQVWQGLEVPYETARVRVLLGVACRALGDDDTAALEL
                     EAARGDFANLGAAPDLARVDSLTRRASPIDAHGLTPREHEVLRLIAAGKTNKAIAAEL
                     VLSERTVDRHVSNIFTKLGLSSRAAATAYAYEHRLV"
     gene            2547..3704
                     /locus_tag="VNT12_05345"
     CDS             2547..3704
                     /locus_tag="VNT12_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWI45579.1"
                     /translation="MNGHIGQQSRRTAAHSEHFDTVIIGGGQAGLATGYHLAKRDLPF
                     VILDANERIGDAWRKRWDSLRLFTPARYDGLQGWRFPAPALSFPTKDEMADYLEAYAA
                     RFELPVRTGVKVDGLSREGDRFLITSGNRRFEAKHVVVATGAYQVPKIPAFADELLSS
                     IMQLHSSQYRRPSQFQEGAVLVVGAGNSGAEIAFEVSRTHPTYLSGKPSGQIPVRHGP
                     AAARFVLPVVRFVGHHVLTLGTPIGRKAHPKFISLSAPLVRVKLKDLAAAGVEQVPRT
                     VSIEDGRPALEDGRVLDVSNVIWCSGFREEFAWIDLPIFDENERPLHERGVVVGEPGL
                     YFVGMPFQYALSSDVLPGVGRDAEYIAKHIARNLTPRGPNDRSLVLAPTGA"
     gene            3780..4340
                     /locus_tag="VNT12_05350"
     CDS             3780..4340
                     /locus_tag="VNT12_05350"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HWI45580.1"
                     /translation="MTFTIDKVNPGLPPAEVALGEAIPILSDLVNDTHFLASHVLPLV
                     DEPAEDWYVAYRHDDPVHSYTVQVFVWPPGSATRVHDHSSWGAFCCVVGSVLEERYER
                     LDDGSLPYHARLKKLWRLEWSREDGISTVLPGDEGIHRVGNPTQEPAISVHLYGPRLA
                     EIDGRDYDPSENYVCDRPQDRWGQMT"
     gene            complement(4420..5601)
                     /locus_tag="VNT12_05355"
     CDS             complement(4420..5601)
                     /locus_tag="VNT12_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HWI45581.1"
                     /translation="MAAVKEPGPEDRTVSPRRRLELLAGVPAFSHLPDPVLEDLASRL
                     TEERFRSADRVVLEGSTDDRLYMIVEGRAEASTIGPSGIVPLATLGPGELFGELSLLE
                     AGSRRQATVAAVEPLLLLGLRAADLRLALDAHPEGRSAFEKLADDLLVTKLLKQASPF
                     STLDGERLRRLAARLGRLEVPTGETIIRQGEAGEECYVLRSGRVEVVTSGAQSDERIL
                     ATLGPGSLFGEAALLTDGPRNATVRAIEPCTLLALRRTDLLEVLGEDRRTRERMLELV
                     RMRDRPRRVRGIEVHHRTTITGETITTLKDARRDTNHRLSPAGWFVWQRLDGEHTLRD
                     LTSEYLATYEASESHAVVEAVVDLVAAGFVEGVKPSPEVMAEEPTLWQRVRATIRRIR
                     E"
BASE COUNT         1078 a         1922 c         1922 g         1090 t
ORIGIN      
        1 ggctcggccg ccccctcgat cccgaccctc cgctagacga gatgatcgta cgctacctcg
       61 gggcgttcgg cccggccaca gtcaaggacg tacagacctg gtcgggcctg acccggctcg
      121 gcgaggtgat cgaacgtatc cggccgcgcc ttcgcatctt ccgcgacgag cgcgggaaag
      181 aactcttcga tgtgcctgac gccccaatgc ccgatcctga tcccccggct ccgccgcgct
      241 tcttgcccga gttcgacaac ctgatcctct ctcacgccga tcgcacccgc gtcatcggcg
      301 aggagtaccg caaagcgatc gcctcgaaga acggcatggt ccctgctacc gtgctcgtcg
      361 acggttttgt ccgcgggacg tggaagaccg agcggagccg tggtaaggcc aacctcgaga
      421 tcaaaccttt cgagcccctg accaaagagg atcgtgacgc cctggccgag gagggagagc
      481 ggctcatccg tttcacaggc gccgaagcct acgagatccg cttcgctaag ccctggaaga
      541 tccacgggac gtcggagcat agccggatcg gacactaact gtcgtcgacg gggtacttcc
      601 agaacaccat tttgattgat acatctacta tactcatccc caaggatagg aggtccttcg
      661 agcgcctcat ggagaaccta caccgcaaag agcatgagac tagcgcgacg atcgccgaag
      721 cgggcggcct ggcggtgcga atcatcctga tccctgctcc aatggactgg ttgtagtagc
      781 cgtggtggat gccaccgacg aactcgaacg gggtcgtgag tcctacgtga ggcgggagtg
      841 gatggatgcc tacgagtcgc tgtcacgcgc cgatcaggcg gctccgctcg gtgcggagga
      901 tctcgaactg ctggcgacgt cggcttatat gctcggccgt gacgacgaca tgagcggcat
      961 ggagcgtgcc tatcacgtct acctcgatgc cggcgaggcg ctgcgtgccg cgcgctgcgc
     1021 gttctgggtg ggtatgcatc tgttcacccg gagagagatg ggccgtggga ccggatggct
     1081 gggtcgcgcg cagcggctgg tcgaacgcga ggggcgtgag tgcgtcgagc aaggctatct
     1141 gaagttgccg ctcatgtttc agcaggaggc tgccggtgac tacgaggccg ctgccgctat
     1201 cgcggccgaa gccgctgaga tcggcgagcg tttccgcgat gctgacctgt tcgccctggc
     1261 cgtacaggcg cagggcatct tcctgatcaa acacgggcgg gtcgaggaag gacttgggct
     1321 gctggacgag gcgatggtag cggtcaccgc gggggaattg tctccgatcg tcagcggtct
     1381 ggtctattgc ggcatgattc taggctgcca ggacgcgtac gagctgcgcc gcgcacagga
     1441 gtggaccgcc gccctcacgc ggtggtgcaa agagcaacca gacatggtgg cattcacagg
     1501 cacgtgcatg gtgcatcgcg ccgagatcat gcagcttcac ggcgcgtggc tggacgcgat
     1561 cgaggaggcg cggcgggccg gcgagcgttc cgcccaggcg atgaatcgga ctgcggtcgc
     1621 caatgcccgc tacctgcagg gtgaggtcca ccgtctacga ggggagtttg ccgctgccga
     1681 agaagcctac caggacgcca gccgcggcgg gtgggagccc cagccgggtc tggccctgtt
     1741 gcgtctggcc cagggagact ccggggccgc gggcgccgcg atccgccgcg ctgtgagcga
     1801 ggcctccgag ccttcgaagc gcgtaggtct ccttcccgcc tacgtcgaga tcatgctggc
     1861 cataggcgat gtccaggagg ctcgcagcgc ctgccgcgag ctcggagaga tctcggcagg
     1921 ttttcagagc ggcatgcttg gtgcgatggc cgcgcacgcc aggggagcga tcgacctggc
     1981 cgacggtgac attcgggctg ccctggttgc gttgcgccac gcgcggcagg tatggcaggg
     2041 gctcgaagtg ccgtacgaga ccgcgcgcgt gcgagtgctt ttgggggtgg cctgccgcgc
     2101 tctcggcgac gatgacaccg ccgcgttgga gctggaagcg gcccgcggcg acttcgcaaa
     2161 ccttggagct gcgccagatc tcgcccgagt cgactctctc acccgccgag cctcgcccat
     2221 cgatgctcat gggctgacgc cgcgtgagca tgaggtgctg cgcttgatcg ccgccggtaa
     2281 gaccaacaag gcgatcgccg ccgagttggt ccttagcgag aggacggtgg acaggcacgt
     2341 gagcaacatc ttcaccaagc tgggcttgtc ctcgcgcgcc gcggccacag cctacgcgta
     2401 cgagcaccgg ctcgtctgaa caccccagga atgggtggaa ttacccattc cggcgcacgg
     2461 tggaagttgg gtagttcctc cgatgtgcct cctcttcgtc gggatcagac tatgggctgt
     2521 ccgaccccga cgaagaggag gcatcgatga atgggcacat cgggcaacag agccggcgga
     2581 ccgctgccca ctcagagcat ttcgataccg tgatcatcgg tggtggtcag gcaggtctcg
     2641 ccacggggta ccacctggca aagcgcgact tgcccttcgt gatcctcgac gccaacgaga
     2701 ggatcggtga cgcctggcgc aagcgctggg actcgctccg cctgttcacc ccggcccgct
     2761 acgacggact gcaaggctgg cgcttccccg cccccgcttt gtcgttcccc acgaaggacg
     2821 agatggccga ctatctcgag gcttacgccg cccgattcga gctccctgtt cgaaccggtg
     2881 tcaaggtgga cggactctcc agggaggggg ataggttcct aattacatcc ggcaaccgcc
     2941 gtttcgaagc gaaacacgtg gtggtcgcca cgggcgccta ccaggtcccg aagattcccg
     3001 ccttcgccga cgagctcctt tcgagcatca tgcagctcca ttcgagccag taccgccgcc
     3061 catctcagtt ccaggaaggc gccgtcctgg tggttggcgc gggcaactct ggagcggaga
     3121 tcgccttcga ggtgtcccga acgcatccca catacctgtc gggcaaaccc tcgggacaga
     3181 tccctgtccg tcacggaccc gctgcggcgc ggttcgtcct ccccgtggtg cggttcgtcg
     3241 gccatcacgt gctgacattg gggacgccta tcgggcggaa ggctcacccc aagtttatct
     3301 cccttagcgc gccgctcgtc cgtgtcaagc tgaaggacct cgccgccgcc ggagtcgaac
     3361 aggtaccaag gaccgtgagc atagaggacg gccgcccggc gctcgaggac ggacgcgtcc
     3421 tggatgtgtc gaacgtgatc tggtgctcgg ggttcaggga ggagttcgcc tggatcgacc
     3481 tccccatctt cgatgaaaac gaacgtccct tgcacgaacg cggcgttgtg gtcggagagc
     3541 ctggcctgta cttcgtggga atgcctttcc agtatgcctt gtcatccgat gtcctccccg
     3601 gcgtcgggag ggacgcggag tacatcgcga agcacatcgc gagaaacctt accccacgcg
     3661 gaccgaacga ccgatcgctg gtcctcgcgc cgaccggagc ataaggtgcc aggcgctccg
     3721 agacgacccg tcaggtgctg gtacctcgta gtcgaaggat cgagcgaaag gaagcacaga
     3781 tgacgttcac aatagataag gtcaacccag gcctacctcc cgcggaagtg gctcttgggg
     3841 aagccatccc catcctctcc gatctggtga acgatacgca cttcctggcc tcgcacgtcc
     3901 tgcccctcgt ggacgaaccg gccgaagatt ggtacgtcgc ataccgccac gacgaccccg
     3961 tgcactctta cactgtgcag gtcttcgtct ggcctccggg ttctgcgacc agggtccacg
     4021 accattcctc ctggggagcc ttctgttgcg tcgtgggatc agtcctcgaa gaacgctacg
     4081 aacgcctcga tgacggctct ctaccctatc atgcccgctt gaagaagctc tggaggctcg
     4141 agtggagcag ggaagacggg atctcgaccg tgctgccagg agacgagggc atccacaggg
     4201 tcggtaaccc gacccaagaa ccggccatct ctgtgcacct gtatggtccc cggctcgcgg
     4261 agatcgacgg gcgggactac gacccttctg aaaactacgt ctgtgaccgg ccgcaagatc
     4321 gatggggtca gatgacataa acagcctgga gagcctggct cgacgactgc tttcgtggag
     4381 gtcggtgagg cactcgacca taccgcgtat cgtagctctc tactcccgga tacgccgtat
     4441 cgttgctcgc acccgttgcc ataaggtggg ctcctctgcc atcacttcgg ggctcggctt
     4501 caccccctcc acgaagcctg ccgccactag atcaactacc gcctccacta cggcgtgcga
     4561 ctcggacgct tcgtacgtag ccaggtactc cgacgtgaga tcgcgcaagg tgtgctcccc
     4621 gtcgaggcgt tgccacacga accagccggc gggggagagg cggtggtttg tatcgcgcct
     4681 ggcgtccttg agggtcgtga tggtctctcc ggttatagtg gtgcgatggt gcacctctat
     4741 cccgcgtacc cggcgcgggc gatcgcgcat ccgcacgagc tcgagcatac gctcgcgcgt
     4801 tcgccgatcc tcgccaagga cctccagcag atccgtccga cgcagcgcga gcaatgtgca
     4861 cggctcgatg gctctcaccg tggcgttgcg tggtccgtcg gtgagcaggg ctgcctcccc
     4921 gaagagggag ccgggaccaa gcgtggccag aatgcgttcg tcactctggg ctccactcgt
     4981 gaccacctct acacgaccgc ttcggagtac gtagcactct tcgccggctt cgccctggcg
     5041 gatgatagtc tctccagtgg ggacctccag acgcccgagc cgggctgcga ggcgccgcag
     5101 ccgctcgccg tccaacgtag agaagggact tgcctgcttg agcaacttcg ttaccaacag
     5161 gtcatctgcc agcttctcga aggctgagcg gccttcggga tgtgcgtcga gtgccaggcg
     5221 gaggtctgct gctcgcaggc ctagcaagag gagaggctcg acggccgcca ccgtggcctg
     5281 ccgcctgctt ccggcctcga gcagggatag ttcgccgaac agctccccag gccctagcgt
     5341 cgccagagga acgatgcctg agggcccgat cgtcgatgcc tcggcccgac cttccacgat
     5401 catatagagc cggtcgtctg tagaaccctc tagcactacc ctatctgcag accggaagcg
     5461 ctcctccgtc aagcgtgaag ccaagtcttc cagcaccgga tcgggcaggt gggagaaagc
     5521 cggtacgcct gcaaggagtt ccaggcgcct cctgggtgag acggtcctat cctccggtcc
     5581 cggttccttc acggcggcca caaacctctt cgaacctccg acgttacttg ccagagatca
     5641 tttaacacca gcccgcgctc ggatcaagac ggagcgggtc gtgccaggca tgcaggagaa
     5701 gattgcaagg gccctggagt aagcacagcg gtggattgtg atgttaacag gcctggccgc
     5761 agtcgcaaag agacctcgta accggctggc taatccgcgt tggtcactgc ggtctgggtt
     5821 cacggggctc ggtacatctc cccggcgcgc aagtcgcagc gctcgccgat cgcctccaga
     5881 gttttggccg acatcggcgg gaaatccggt agctgcatag taaatgcctc tcgtgtccgg
     5941 gttcagagag tgcagaatat acatgaggtt ggcgacaggc ttccgtacgc ggcatcatta
     6001 tctacctggt tc
//
LOCUS       DATMJA010000226         3844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_30068, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000226 DATMJA010000000
VERSION     DATMJA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30068"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VNT12_05360"
     CDS             complement(<1..274)
                     /locus_tag="VNT12_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45582.1"
                     /translation="MVDLADKVSESAWKIKNLKDYHGRLERAVRKFDEISRERPEPAL
                     VVYASALKKSGRSEDEIRGMVREFYDTFEDEEEPLTITRASAIMSVE"
     gene            complement(331..555)
                     /locus_tag="VNT12_05365"
     CDS             complement(331..555)
                     /locus_tag="VNT12_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWI45583.1"
                     /translation="MDFKAVARKVLEEVGHPLHYTDITEIALQSGYLQSAGHTPQNTM
                     RARLSVDVRDNPATPFVHVAPGVYGLKNAS"
     gene            complement(570..1709)
                     /gene="pheA"
                     /locus_tag="VNT12_05370"
     CDS             complement(570..1709)
                     /gene="pheA"
                     /locus_tag="VNT12_05370"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWI45584.1"
                     /translation="MFPPEASRENTTHDLEVPEAPDEMQTLRDGIDSVDEEIVRLLDR
                     RAWLARRIGEIKHKNGLAAYAPARERAVLDRVATLGDGEFPRRGLQAVFREIISSSIS
                     LEARLKVAYLGPEATFTHEAALRSFGTSIELEPQTTVAEVFVRVERGEVEHGVVPVEN
                     SMEGAVTHTLDELMNSPLKICGEVYLPVSQNLISAEQSMERVSLVCSHPMALAQAASW
                     LRRELPGARLDEVDSTAEAARRAAVEPGVAAIGSVLAAGAHGLTVLARNIQDARANTT
                     RFIVLGRTWAARTGKDKTSVVFSVKDRPGVLRDALSAFAEEGINLTRIESRPSRKRAW
                     TYVFFADFLGHPEEERVKRALEALEEHCPYVGLIGAYPEVTSEVV"
     gene            complement(1867..2697)
                     /gene="uppP"
                     /locus_tag="VNT12_05375"
     CDS             complement(1867..2697)
                     /gene="uppP"
                     /locus_tag="VNT12_05375"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HWI45585.1"
                     /translation="MLELVEAILLGVVQGLTEFLPVSSSGHLLLGQYFLGMDQDRFGL
                     TFDASIHTGTVLAVVSFFWRDLLRMAAAFVGSLRGPRFADRDQRMAYLILVACIPAGV
                     VGLLFQNFFEDEVRSPWLVAFNLVLVGALFILGEEVGRQTRTSDKLGFKEAIGIGLAQ
                     TAALFPGVSRSGATITIGLFLGLRREEAARFSFLMSVPITTAAALLSLADAFGSGIDA
                     GDVLLFLVGSITSGVVGYLAIRFLLNYLTGHSLLVFAYYRFALAAVVVVLLLFGIGGF
                     "
     gene            complement(2837..3532)
                     /locus_tag="VNT12_05380"
     CDS             complement(2837..3532)
                     /locus_tag="VNT12_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWI45586.1"
                     /translation="MAVDISLTEYTKLQEQECRSLLQLLGEMGIATADRVYGSGDAIY
                     NEGEYGDALYVLTSGVLKLFRPYSGSKEATLRLLKPWDIFGHLAFAGETRQRAYAEAV
                     TECRVTKVPKIFVERAVRQEPRAAFKLMTLLELRLVQYEELVKCLLPRETEVRLANLL
                     PLLAKKFGQNRDGLVTIDLRLTHQDLAAMVASTRESVTKVLNEMRNRDVIEVEAGRIT
                     LKDPAALAVLSGV"
BASE COUNT          724 a         1246 c         1175 g          699 t
ORIGIN      
        1 cttcgacgct catgatggcg ctcgcgcggg tgatggtcag cggctcttcc tcgtcctcga
       61 atgtatcgta gaactcgcgg accatgccgc ggatctcatc ctcactgcgt ccgctcttct
      121 tgagtgcgct cgcgtagacc accagcgccg gctcagggcg ctccctggat atctcgtcga
      181 acttgcgcac cgccctctcg agccgtccgt ggtagtcctt taggttcttt atcttccagg
      241 ctgactcgct gaccttatcc gctaaatcga ccatggtctc tcctaggctc tcaaagaatc
      301 gttcctcgca atatagcatc tcggtttgcg tcaggacgca ttcttcagtc cgtagacgcc
      361 gggtgcgacg tgcacgaatg gcgtagccgg attgtccctg acatcaacgg agagcctcgc
      421 cctcatcgta ttctggggcg tatggcctgc actctggagg taccctgact gcaacgcgat
      481 ctccgttatg tccgtgtagt ggagaggatg gccgacttcc tctagaacct tcctcgcgac
      541 ggccttgaag tccacaccgc ccctggccgt cagactactt cggaagtgac ttcggggtag
      601 gccccgatca ggccgacgta agggcagtgt tcctcgaggg cttcgagggc gcgcttcacc
      661 ctctcctcct cgggatgacc gagaaagtcc gcgaagaaca cgtaggtcca ggcacgcttg
      721 cggctcgggc ggctctcgat acgggtaagg ttgatcccct cctccgcgaa agccgagagc
      781 gcgtccctga gaactccggg gcggtccttg acggagaata cgacgctggt cttgtccttg
      841 ccagtccgcg cggcccacgt gcgccccagc acgataaagc gcgtggtgtt cgcgcgggcg
      901 tcctggatgt tgcgcgccag caccgtcagg ccgtgggcgc cggccgcaag cacgctcccg
      961 atcgcggcca cccccggctc gactgccgcg cggcgggccg cctccgccgt ggaatcgacc
     1021 tcatccaggc gggctccagg gagctcgcgc ctgagccagc tcgcggcctg ggcaagggcc
     1081 atgggatgcg agcacaccag gctcacccgc tccatcgact gttctgcgga gatcaaattc
     1141 tgggagacag gcaggtagac ctccccgcag atcttcagtg ggctgttcat cagctcgtcc
     1201 agggtgtggg tcacggcacc ctccatggag ttctcgacgg gcacaacgcc atgttcgacc
     1261 tccccgcgct cgaccctgac gaagacctcg gcgaccgtcg tctgaggctc gagctcgatg
     1321 ctggtgccaa acgaacgcag ggcggcctcg tgcgtgaacg tcgcctcagg cccgaggtaa
     1381 gcgaccttga ggcgggcctc gagcgagatg gaggatgaga tgatctcgcg gaacacggcc
     1441 tgcagtccac gcctgggaaa ctccccatcg cccagcgttg cgacacgatc gaggacggcc
     1501 cgctccctgg caggggcgta tgccgcaagg ccgtttttgt gcttgatctc acctatcctc
     1561 cgcgccagcc aagcacgcct gtcgagcaga cgcacgatct cctcgtccac cgagtctatg
     1621 ccatcgcgca gggtttgcat ctcgtccgga gcctcgggaa cctccaggtc gtgggtggtg
     1681 ttttctcttg atgcttcggg tgggaacatg ggtgtgcgtc cttcggtagg tgccgtgttt
     1741 gggctcaggg taggaggagc atggccgcgc gtccgggtcg ggcacacccc tcctcgataa
     1801 atcgccggaa cgcacagaac gcgagattca tattggcctc aaagtaccag aagagcgcgg
     1861 cgcctgtcaa aagcccccga tgccgaacaa caggagcacg accaccacgg cggccagcgc
     1921 gaaccggtag taggcgaaga cgagcaggct gtgcccggtg aggtaattga gaagaaacct
     1981 tatggctagg tacccgacga cccccgaggt gatgctgccg accaggaaga gcaacacgtc
     2041 gccggcgtct atccctgacc cgaaggcgtc ggcaaggctc aagagcgcgg cggcggtcgt
     2101 gatggggacg ctcatcagga aagagaaccg cgccgcctcc tcccgccgga ggcccagaaa
     2161 gagcccaatc gtgatcgtcg cccccgagcg cgagacgccg ggaaacaggg ccgccgtctg
     2221 ggccaggccg ataccgatcg cctccttgaa gcccagcttg tcggaggtgc gggtctgccg
     2281 acccacctcc tcgcccaata tgaagagcgc gccgaccagc accaaattga acgcgacgag
     2341 ccacggcgag cgcacctcgt cctcgaagaa gttctggaac aagagcccga cgacgcctgc
     2401 cgggatgcag gcaaccagga tcaagtacgc cattcgctgg tcccggtccg caaacctcgg
     2461 acccctcagc gagcccacga aggccgccgc catgcgcaag aggtcccgcc agaaaaacga
     2521 gacgacggcc agcacggtac ccgtgtggat cgacgcgtcg aacgtaagcc cgaagcggtc
     2581 ttgatccatg ccgagaaagt attgcccgag caacaggtga cctgagctgg aaaccggaag
     2641 gaactccgtg agcccctgca ccaccccgag aagtatcgcc tcaactagct ctagcacgcg
     2701 gccatattag ccgatccgcg tccggtttcc agcggccact cgacgggcgg tccccgccga
     2761 atacagtcct ctatctcttc gacgggccag gtgtaaggtt cgttccggga gaactctcct
     2821 ggaaggtagc ccgcctctaa accccgctta gcacggcaag ggctgcgggg tctttgaggg
     2881 tgatgcgccc cgcctcgacc tcgatcacgt cgcggttacg catctcgttg aggaccttgg
     2941 tgaccgattc cctggtagag gcgaccatgg cggcgaggtc ctggtgggtt aaacgaaggt
     3001 ctattgttac gaggccgtcc ctgttctggc cgaacttctt cgccaagagg ggcagcaggt
     3061 ttgcgagccg cacctccgtc tcgcggggca atagacactt gacaagctcc tcgtactgca
     3121 cgagacgcaa ctccagaagg gtcatgagct tgaaagcggc ccgcggttcc tggcggacgg
     3181 cgcgttcgac gaagatcttg ggtaccttgg tcaccctgca ctccgtaacg gcctcggcgt
     3241 aagcgcgttg cctggtctcc ccggcgaagg ccagatggcc gaagatatcc cagggcttga
     3301 gcaagcgtaa cgtcgcctcc ttgctacccg agtagggcct gaacagcttc agcacgcccg
     3361 aggtcagcac gtagagggcg tcaccgtact cgccctcgtt gtagatggcg tcccctgatc
     3421 cgtagaccct gtcggccgtg gcgatgccca tctcgccgag cagctgcaac agcgaccggc
     3481 actcctgctc ctgcagcttc gtgtactcgg tcagggaaat atcaacggcc acgtgggtcc
     3541 cctccctgga attccgttcc gagaccacac cgatactcac gaacctcggg actgagcgtg
     3601 ctacacattg ctacattgta cattacgcgc gggcggacat tagtgcgttc cgcaggacgt
     3661 gccttgcgga ttgggttaga atggcgcgga ggttcgtatc cgagagagga gacaaggtgc
     3721 gggagcggct ggcattcggg atcctgatcc tgctcctgat ctgcctgttc gccttcacct
     3781 tcgccgtcct cggaaagggt gagtacgaac cgactggaga gaagccccag caggtatctc
     3841 aaag
//
LOCUS       DATMJA010000227         3301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_385777, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000227 DATMJA010000000
VERSION     DATMJA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385777"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1035
                     /gene="sucD"
                     /locus_tag="VNT12_05385"
     CDS             160..1035
                     /gene="sucD"
                     /locus_tag="VNT12_05385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWI45587.1"
                     /translation="MAILVDDGTRLLVQGITGREGAFHTGRMLQSGTRVVAGVTPGKG
                     GQDYEGVPVFDTVEEAVEQTGANASVIFVPPPFAADAIFESLDAGLSTICCITEGIPV
                     HDMMRVANYLVTKDATLVGPNCPGVLSPEKANVSIMPKDIFMPGNVGVVSRSGTLTYQ
                     IVNELTQREIGQSTAVGIGGDPIIGTDFIEILSKFEADPETECAVMIGEIGGNAEQLA
                     AEYVSSEMSIPVVAYIAGFKAPEGKTMGHAGAIVSGGESTAEAKRESLREAGIRAATN
                     PSEVAELVAEALGRG"
     gene            1160..3109
                     /locus_tag="VNT12_05390"
     CDS             1160..3109
                     /locus_tag="VNT12_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HWI45588.1"
                     /translation="MAESTISKQQAGRRFVRWLLEERIEEVQGPEVREGEKQHAWWQV
                     VCLTGVDYFSTLGYIPGIAIATAGALSPVATLLIVLLTLFGMLPMYRVVAAESPHGQG
                     SIAMLERLLSFWKGKLFVLCLLGFVATSWVITITLSASDATAHVVENPFWPEALRDER
                     VLITLALLAVLGGIFLKGFTEAIGIAVAVVGAYLLVNFVVVAAGFYEIAIQPQVLVDW
                     QSALFRENGSVLGMIGVALLVFPRLALGLSGFETGVSMMPLVRGDADDDPERPEGRIR
                     NTRKMLLAAALIMCFYLITTSFVTTLLIPESEFGPEGEAQGRALAYVAHEHLGNAFGT
                     IYDISTITILWFAGASAMAGLLNVVPRYLPRYGMSPEWGRAVRPLVLVYTAISMIVTV
                     IFVADVGSQAGAYATGILAMMTSAGFAVTLTAWRGGRKPGTVLFGIVTVVFVYALASN
                     VYQRPDGITIALFFIGAIIAISLVSRIQRSTELRQERIDVDEKARGFIEEAAQRGEIH
                     LVAHRRRNGTEEEYSRKEREQREDNHIPREDQILFLEVQVEDASEFTDVLEVNGVEVG
                     VHQVLRCVSSVVPNAIAAFLLHLRDTTGKTPHCYFGWTEGNPIVYLIRYILFGEGDTA
                     PVTHEVLREAEPNPERRPAIHVGGR"
     gene            3106..3252
                     /locus_tag="VNT12_05395"
     CDS             3106..3252
                     /locus_tag="VNT12_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45589.1"
                     /translation="MIRQFVVIGLGRRSLPRCDTILHDGDVLVVGGSKKALDRSPLVE
                     PDGP"
BASE COUNT          626 a          997 c         1087 g          591 t
ORIGIN      
        1 tcacgctccc catcgtcgtc aggctcgacg gtacgaacgc cgaagaggga cgccggatcc
       61 tcgccgagaa cacaccggag aacgtggtca cagaggagac gatgctggaa gcggcccgca
      121 gggcggtaga gctcgctaga gacgggggga gataacttca tggcgatact cgtagacgat
      181 gggacgcggc tcctcgttca gggcataacg ggcagggagg gcgcgttcca cacaggaagg
      241 atgctacaga gcggcaccag ggtggtcgca ggggtgacgc cgggtaaggg cgggcaggac
      301 tacgaaggag tccctgtctt cgacaccgtg gaggaggccg tcgaacagac gggcgcgaac
      361 gcgagcgtga tcttcgtccc cccacccttc gccgccgacg ccatcttcga gtctttggat
      421 gcgggactct cgactatctg ctgcattaca gaaggcatcc ccgtacacga catgatgcgg
      481 gtcgcaaact acctggtgac caaggatgcg accctcgtcg ggccgaactg tcccggcgtc
      541 ctctcgccgg agaaggcaaa cgtctccata atgcccaagg acatctttat gcctggcaac
      601 gtcggcgtcg taagccgcag cggaacgctg acttaccaga tcgtcaacga gctcacgcag
      661 cgggagatcg ggcagagcac cgccgtcggg atcggcggcg accccatcat agggacggac
      721 ttcatcgaga tcctgagtaa gttcgaggcc gaccccgaaa cggaatgcgc ggtgatgatc
      781 ggggagatcg ggggtaatgc ggaacagctc gcggccgagt acgtcagcag cgagatgagc
      841 atccctgtcg tagcctatat cgcgggtttc aaggcccctg agggcaagac catgggacac
      901 gcgggcgcca tcgtctctgg cggtgagagc acggcagagg ccaagagaga gtcgctcagg
      961 gaggcaggca tcagggcagc gacgaatcca tcagaggttg ccgagctggt cgccgaagca
     1021 ctcggccggg gctgaagcac agggaggcct ggaggacgaa gaggtctgac ctccgatacc
     1081 ttatacttcc tgggctcacc atgaacgggt atcgtccaac ccaggcaagc cggacctcct
     1141 cgaccgggac aatggcgtag tggccgaatc caccatttcg aagcagcagg ctggcagacg
     1201 gttcgtgcgc tggctcttgg aagagcgcat cgaagaggtg caggggcccg aggtcaggga
     1261 gggagaaaag cagcatgcgt ggtggcaggt cgtgtgcctg actggcgtgg actacttctc
     1321 cactctaggc tacataccgg ggattgcgat cgctaccgca ggcgcgctat cacccgtcgc
     1381 caccctgctg atcgtactgt tgacgctctt tgggatgttg cccatgtacc gcgtcgtcgc
     1441 tgcggagagt ccgcatgggc agggttcgat cgccatgcta gagaggttgc tttcgttctg
     1501 gaaaggcaag ttgttcgtcc tgtgcctgct cggtttcgtg gcgacctcct gggtcatcac
     1561 catcaccctc tcggcgtctg acgccacggc ccacgtggtc gagaacccgt tctggcctga
     1621 ggccctgcgg gatgagagag tgctcatcac cctggcactg ctcgcggtgc tcggtgggat
     1681 cttcctcaag ggattcacgg aggcgatcgg catcgcggtc gccgtggtcg gggcctatct
     1741 gctggttaac ttcgtggtcg tagcagccgg cttctacgag atcgccatcc aaccccaggt
     1801 tctggtcgat tggcagagcg ctcttttcag ggagaatgga agcgtgcttg gcatgatcgg
     1861 ggtagcgctc ctcgtatttc ctcggctcgc cctcggcctc tccggcttcg agaccggcgt
     1921 tagcatgatg ccgctggtgc gcggagacgc cgacgacgac cccgagcgtc cggagggacg
     1981 catacggaat acccgcaaga tgctcctggc tgcggccctg atcatgtgct tctacttgat
     2041 taccacgagc ttcgtgacga ctctcctgat accggagagc gaattcggtc cggagggcga
     2101 ggcccagggc agggccctcg cctacgtggc gcacgagcat ctgggcaacg cgttcggcac
     2161 gatctacgac atcagcacga taaccatcct gtggttcgcc ggcgcctcgg ccatggcagg
     2221 gctgctgaac gtggtgcccc gctatctacc ccgctacggc atgtcgcctg agtggggcag
     2281 ggccgtcagg ccgctggtac tcgtctacac cgccatctcc atgatcgtca cggtcatctt
     2341 cgtcgccgac gtgggttcgc aggcgggggc ctacgcgaca ggcattctgg cgatgatgac
     2401 ctcggcgggc ttcgccgtca cgcttaccgc ctggcgcggc gggcgcaaac cgggaacggt
     2461 cctcttcggg atagtaaccg tcgtcttcgt ttacgcgctg gcctccaacg tctaccaacg
     2521 tcccgacggc atcaccatag cgttgttctt tatcggggcg atcatcgcta tctctttggt
     2581 atcccgcatc cagcgctcga ccgagctcag gcaggagcgg atagacgtgg acgagaaggc
     2641 taggggtttc atcgaggagg cggcgcagag gggcgagata catctggtcg cccaccgtcg
     2701 ccgcaacggc accgaggagg agtactccag gaaggagagg gagcagcgcg aggacaacca
     2761 catcccgcgt gaggatcaga ttctgttcct ggaggtacag gtcgaggacg cctcggagtt
     2821 cacggacgtg ctcgaggtaa acggcgtcga ggtcggagtt caccaggtgc tgcgttgcgt
     2881 aagctccgta gtccccaacg cgatcgcggc cttcctccta catctgcgcg acacaacggg
     2941 gaagacgccg cactgctact tcggctggac agagggcaac cccatcgtgt acctgatccg
     3001 ctacatcctc ttcggtgaag gcgataccgc gcccgtcacc cacgaggtac tgcgcgaggc
     3061 ggagccgaac ccggaacgac gccctgcgat ccacgtcggc ggccgatgat ccggcagttc
     3121 gtggtaatcg gcctggggcg ccggtccctg ccccgctgcg acaccatact gcacgacggg
     3181 gacgtgctcg tggtcggagg atccaagaag gccctggata gatccccact cgtagagccc
     3241 gacggtccgt aggaggtttc ccgctttgca cacggtgatc ataggcgccg gggaggtcgg
     3301 g
//
LOCUS       DATMJA010000228         2913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_89487, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000228 DATMJA010000000
VERSION     DATMJA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89487"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="VNT12_05400"
     CDS             <1..1538
                     /locus_tag="VNT12_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWI45590.1"
                     /translation="DGAGTVFWSRSAEVVMRYPLAVILLAAAILGTLLYPVGHMKVGI
                     PEATVLPQEYESRAGDDILKKNFEYAALNPMEIVATLEDDPLSVRGLSDTRELGERIG
                     AADGVSRVESVYTIAAAAAENYAGRVANARKQARKDAAAKVDGLVQRQIEEQTQNETD
                     RAVNEQLSQIEATYGAVPPGTEERLRADIRPRVEREVRASEGRIRDEVERRLAEEVDR
                     KVPGLPEGISADGRVTPEGVANFLKLPAARRSEDLQDTLDTYVAGDRTLLRGVTEANP
                     YTQDAYGTVDAVRAVEAPDGTSFLVGGLSAGQKDFISSLYGKAPFAAAFVIGVTYLIL
                     LITFRSVFIPLKAVIVNILSLTASFGTMVFVFQDGHLSGLLDFTPLGFVDATLPILMF
                     CTIFGVSMDYEVFLLSRIREAYENGDSNTASVAKGLVATAGIITSAAAIIIVVTGAFA
                     LTGIVLTKAIGLGLAVAVFVDATIVRILLVPATMRILGDWNWWPGGRKVTFGTGPKEP
                     PRN"
     gene            1639..2052
                     /locus_tag="VNT12_05405"
     CDS             1639..2052
                     /locus_tag="VNT12_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWI45591.1"
                     /translation="MLDFERILKDRGYRLTNQRRIIVHELEGERHLSAEEIYDRVKGG
                     YPELGLSTVYRTLDLLTELGIVRKDDFGEGYSRYELATERMHHHARCEHCGNVIEFNE
                     ELMEYLALQVERETGFVTDWHEITLYGRCADCAVS"
     gene            complement(2049..2828)
                     /locus_tag="VNT12_05410"
     CDS             complement(2049..2828)
                     /locus_tag="VNT12_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI45592.1"
                     /translation="MTTEGRNMEITVVGSGTVVPRLERRQSCVVARAGGQTVVFDLGS
                     GAVRGMLRSGLDPLAVDRIFFTHFHPDHTVDVVPLLFAINYGAPEKRTLPLYVTGPEP
                     FLRFWGSVMEVWGKWMTGEHATLVSEVPHECPSPIHLAGCRLSWAPAEHRRESIAYRL
                     DADGGPFVYTGDTEYSQSVIELARGADTLLIECSFPDEAPVPGHLTPSGVARIASEAG
                     VRRVVLTHIYPAAEDLDLVTEVGRGFEGEILVAQDGLEFEV"
BASE COUNT          555 a          874 c          944 g          540 t
ORIGIN      
        1 gggatggggc gggcacagtg ttctggagcc gcagcgccga ggtcgtgatg cgctatcctc
       61 tcgccgtcat cctgctcgcg gccgcgatcc ttgggaccct gctctacccg gtcggccaca
      121 tgaaggtcgg catccccgag gcgaccgtct tgccgcaaga atacgagtcc cgcgccggag
      181 acgacatcct gaagaagaac ttcgagtacg ccgcgctcaa cccgatggag atcgtcgcca
      241 ccctggagga tgatccactc agcgtccgcg gtctctccga cacgagagaa ctcggcgaga
      301 gaatcggcgc cgccgacgga gtctcccgcg tcgagagcgt atacacgata gcagcagcgg
      361 ctgcggagaa ctacgcagga cgagtcgcca acgcccgcaa gcaggcgcgg aaggacgccg
      421 cagcaaaggt ggacgggctc gtgcagcggc agatcgaaga gcagacgcag aacgagacag
      481 acagagccgt gaacgagcaa ctctcacaga tagaggctac ctacggcgcc gtccctccgg
      541 gaaccgagga gcggctgcgg gcagatatcc ggccgcgggt ggagcgcgag gtgcgcgctt
      601 cggagggccg tataagggac gaggtcgagc gccgcctggc cgaagaggtg gaccgcaaag
      661 tgccggggtt gcccgaaggg atctcggccg atggcagggt cacgccggag ggtgttgcga
      721 acttcctgaa gttgccggcg gccaggcgat ccgaggacct gcaggacacc cttgatacat
      781 acgtggcggg agatagaacg ctgttaaggg gggtgaccga ggcgaatccc tacactcagg
      841 acgcatacgg gacggtcgat gcagtgcgcg cagtcgaggc gccggatgga acgagcttcc
      901 tcgtcggcgg gctctccgcc ggccagaaag acttcatatc cagcctgtac ggcaaagcac
      961 ccttcgccgc cgccttcgtt atcggggtga cgtacctgat actgctcatc accttccgct
     1021 ccgtattcat cccgctgaag gcggtcatcg tcaacatcct gagccttacg gcgagcttcg
     1081 ggactatggt cttcgtcttc caggacggcc atctctccgg cctcctcgac ttcacgcctc
     1141 taggcttcgt ggatgcgacg ctcccgatcc tgatgttttg tacgatcttc ggggtctcca
     1201 tggactacga ggtcttcttg ctctcccgca tcagggaggc ctacgaaaac ggcgactcca
     1261 acaccgccag cgtcgcaaag ggtctggtgg cgactgcggg tatcatcacc tcggccgccg
     1321 ctatcatcat agtggttacc ggggctttcg ctttaacggg tatcgtcctg accaaagcta
     1381 tcggcctcgg cctcgccgtc gccgtcttcg tcgacgcgac gatcgtccgt attctcctcg
     1441 tccccgctac aatgcgcatt ttgggtgatt ggaactggtg gccaggaggc cgcaaggtta
     1501 cctttgggac tggcccgaaa gagcctccta ggaactgagc gcccgataga gggatgcacg
     1561 aaactcttcg aacgcgacga gcccttgaga taagatcgat tcttgatctt gctgcgtgag
     1621 ggggatagac tgtggctgat gttggatttc gagcggatac tcaaggacag gggctaccgg
     1681 ctaaccaacc agcggcgaat catcgtacat gagctggagg gggagcgtca tctttcggcg
     1741 gaggagatct acgaccgggt caagggcggg tatcccgagc ttggtctctc cacggtgtac
     1801 cggacgctcg acctcttgac cgagctcggc atcgtgcgca aggatgattt cggagagggt
     1861 tactcgcgtt acgagctggc gacggagcgg atgcatcacc acgcgcgctg cgagcactgc
     1921 ggcaacgtca tcgagttcaa cgaggagctg atggagtatt tggcccttca ggtcgagagg
     1981 gagaccggtt tcgtcacgga ttggcacgag atcacactgt acggccgctg cgccgattgc
     2041 gcggtctctt aaacctcgaa ctccagaccg tcctgggcaa cgagtatctc gccttcgaaa
     2101 ccccttccta cttcggtgac gaggtccaga tcttccgccg ccgggtagat atgggtgagg
     2161 acgacacgac gtacacctgc ctcgctggcg atacgtgcca cgcctgaagg cgtcaggtgg
     2221 ccgggaacgg gggcttcgtc ggggaaggaa cactcgatca gtagtgtatc ggcgccgcgc
     2281 gccagttcga ttacggactg gctgtactcg gtgtcacccg tgtatacgaa cgggccgccg
     2341 tcggcatcca gcctgtaggc gatgctctcc cggcggtgct ccgcaggggc ccacgaaagc
     2401 cggcagccgg cgagatggat gggggatggg cattcgtggg gcacctccga gacgagcgtg
     2461 gcgtgctctc cggtcatcca ctttccccag acctccatca cggaccccca gaatcggagg
     2521 aagggctcgg gaccggtcac gtaaaggggc agcgtgcgtt tctcgggtgc gccgtagttg
     2581 atggcgaaga ggagaggaac gacgtctacc gtatggtctg gatggaagtg cgtaaagaag
     2641 atgcggtcta cggcgagggg atcgaggccc gacctgagca tgccgcgcac cgcgcccgaa
     2701 ccgaggtcga agacgaccgt ctgcccgccg gcccgcgcta cgacgcagct ctggcgcctc
     2761 tccaggcgtg gaactacggt gcccgatcct acgacggtga tctccatgtt cctcccttcg
     2821 gtcgtcacag catttcagct tgtcaggtct tttctctgtt ccggtatatc gagtgctgtc
     2881 ttggctggtt gctcttcgaa cgcccaggcg agt
//
LOCUS       DATMJA010000229         5619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_89528, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000229 DATMJA010000000
VERSION     DATMJA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89528"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..774)
                     /locus_tag="VNT12_05415"
     CDS             complement(133..774)
                     /locus_tag="VNT12_05415"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HWI45593.1"
                     /translation="MSKGARWMLAVTVGLLASIGVGASAVHYLQEPYNPGFLDFPTIV
                     ALHVILGGVYLTLAPFQFVKGIRSRHLTYHRWTGRLLVAIGVVVGVTALFMGLVIPKA
                     GWPERVVIGFFGGLFSIALVKGFLHVRAGRVALHREWMIRAFAVGLAIATARLIFFPA
                     LLITTADPTDGLFGTLLVVALAVAFVLNTSAAELWIRATRRSGSPTASAVKAE"
     gene            complement(838..1731)
                     /locus_tag="VNT12_05420"
     CDS             complement(838..1731)
                     /locus_tag="VNT12_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWI45594.1"
                     /translation="MNTTVIRREDEAAIRQLVQNMQDGQNRKDGELFASAFAQEHDYI
                     AINGMFLPNQRRKDNARIHQRLYDESTSSVAGNYGEVEIRLSVSKIRPLTSQGVAVVH
                     ILSEFHLEGQPEKKTKNVITAVMQKREGEWEILAFHNAPVQKREEEETGFVIHIEGVD
                     TRQGGREMIKDVPLIGVFVDDQKAALDFYTGKLGLEKVQDEAYGPDARWITVSPAGMR
                     MRIVLKKAEKEYEKAMVGNSDGAPVLTLGTDDVRAAYERLGERGVRFLGEPYRYPWGI
                     GALLLDQDGSPILLQQELGEE"
     gene            1883..2896
                     /locus_tag="VNT12_05425"
     CDS             1883..2896
                     /locus_tag="VNT12_05425"
                     /inference="COORDINATES: protein motif:HMM:NF039924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5937 family protein"
                     /protein_id="HWI45595.1"
                     /translation="MITIPLTTEDLTKVRLAPSPLWETVTSFGVLLHQGRDTVHAPWA
                     ARARRVLPGTDLSALVAAMCVAGRCPDFLTPPPDASATTFGEELERLRTTPSEVVHKE
                     VEAFVRVEEEQFASLAPEKERMLKAYLKDPEDSLRRLVDALVRYHDAAFAPYWPRIRE
                     HLEGDTLRRGQALALGGVEALLSGLHPKARYSGGVLELDKAYEAIIEPAGRGITLVPS
                     VFAWPRVEVLVQPGYRPTLAYGPRGAAKLWSSSSPATNGTALEAALGAGRASVLKGLL
                     PVPSTTTELAQQLHLSPASVSAHLSRLKAAELVEPHRSGRRVYYRLSCAGESLLGIFG
                     ETG"
     gene            complement(2967..3200)
                     /locus_tag="VNT12_05430"
     CDS             complement(2967..3200)
                     /locus_tag="VNT12_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45596.1"
                     /translation="MQATVGIALGVLGVFCAVLGLFVINGVSIEFPGIILGGLGYYFG
                     PQGANRTGQILGIVAVVLSVISIVVSGVSGGPQ"
     gene            complement(3204..3875)
                     /locus_tag="VNT12_05435"
     CDS             complement(3204..3875)
                     /locus_tag="VNT12_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45597.1"
                     /translation="MREEFMITRQGKQYVLYSGLLDEAHSLGLTGIDTDLIQVPDESN
                     GNVAIVKATAEMEDGKRFSGIGDASPENVGRNIAPHLIRMAETRAKARALRDAVNVGA
                     TALEELSDGDDAPPVSQSSPSRGRPTPIRGGRQNSSQDSSRSAAAEQDTPNGQPEKPA
                     SSKRGGAGKARKSQVDLLKTLAVEWRGENGVERLENRLGKPLKDMTRAEADEWIDRLT
                     PEGRE"
     gene            complement(3872..4702)
                     /gene="zapE"
                     /locus_tag="VNT12_05440"
     CDS             complement(3872..4702)
                     /gene="zapE"
                     /locus_tag="VNT12_05440"
                     /inference="COORDINATES: protein motif:HMM:NF005992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HWI45598.1"
                     /translation="MSGSSGRRSKRDAGAEVLRLDDKICPHCGRELQAEWDEFPPALQ
                     KKYGKPGEWYYHPCTPECEKKNEQREWELMRRDARVATLRDRSGLSKRMKGYALDNFD
                     PGFSRNSQKAFAKVMDYIMGWKANQEGGRGLYFCGPVGVGKTHLAVAVMNEIITKKRV
                     PSLFVTVPELLDNMRGAYNDPGRDIDEWMDSVKNADLLLLDDLGAERANEWVRERIFV
                     IVNHRYREELPTIFTSNTGPKDLAAQLGERTASRIIAMCDWISLEGEDYREAEVRRGL
                     "
     gene            complement(4674..5423)
                     /locus_tag="VNT12_05445"
     CDS             complement(4674..5423)
                     /locus_tag="VNT12_05445"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HWI45599.1"
                     /translation="MERVRLTPPGENRARGGRARLYVRWMPHLGAEAVALYELLRILP
                     ELGNDAPEIEELAELLRSTPEGVEASLEALIEARFVVRRDGWLEVAEHPPSAQTSERP
                     SEDELPVRDVEVIRAEPEGVSADDYFRFMGTLPAPHILEFLNGYCGRDGLTPDAVREA
                     LKIASERDARRVGYVRSILERWVERGVKTPEDVERFEQDRKLRLVEQSGAVRGGALRI
                     REAGEGVSGGPDRQSAARRREGYEWLFGETE"
BASE COUNT          967 a         1891 c         1683 g         1078 t
ORIGIN      
        1 tcatattcga ggtgttccat ggcgtggcgc agcatggtgg cgccatgttc gtccaggacc
       61 tcaaagtctc gggcgatatc cggagaaaac aggccgatct ttgttcaaaa ccttgctcga
      121 agtggacggg cttcattcag ctttcaccgc gctagctgtg ggggagccgc ttctgcgggt
      181 ggcgcggatc cagagctccg ccgccgaggt gttgagtacg aaagccacag ccaacgccac
      241 gaccaagagc gtcccgaaga gcccgtcggt cgggtcggct gtggtgatga gaagggcggg
      301 gaagaagatc agacgcgccg tggcgatggc gagtcctacg gcgaacgccc gaatcatcca
      361 ctccctgtga agcgccaccc ttcccgcgcg aacgtgaaga aagcctttga ccagggcgat
      421 ggagaacagg cctccgaaga atccgatgac caccctctcg ggccagccgg ccttcgggat
      481 caccaacccc atgaacaagg ccgtaacccc gaccaccaca ccgatggcga cgagcaacct
      541 tccggtccag cggtggtagg tgagatgccg agacctgatc cccttcacga attggaacgg
      601 ggcgagggtc agatagacgc cgccgaggat cacgtgcagc gccacgatgg tcgggaagtc
      661 cagaaacccg gggttgtacg gctcctgaag gtagtgaacg gcagaggccc cgacaccgat
      721 agacgccagg aggccgaccg tcacagccag catccatcgt gcgcccttgc tcatcgttgg
      781 agccctttcc tgcgaccaga tggacgcatc acctccgagt atcgtgcgga gacgttgcta
      841 ctcttcgccc agctcctgct gcaggagaat ggggctgcca tcctgatcca agagcagcgc
      901 accgatgccc cacggataac ggtaaggctc cccgagaaac cgcacgcccc gctccccgag
      961 ccgctcgtag gccgcccgga cgtcgtccgt gccgagagtc aggaccggcg ccccgtcgga
     1021 gttgccgacc atcgcttttt cgtactcctt ttcggccttc ttcaggacga tcctcatcct
     1081 catccccgcg ggcgagaccg tgatccaacg ggcatcaggg ccataagcct cgtcctggac
     1141 cttctcgagg ccgagcttgc ctgtgtagaa gtccagggcg gccttctggt cgtcgacgaa
     1201 gaccccgatg agtgggacgt ccttgatcat ctccctacct ccttgtcttg tgtccacacc
     1261 ctcgatgtgg atcacgaacc ccgtctcttc ctcttcccgc ttctggacgg gagcgttgtg
     1321 gaaggccaag atctcccact cgccctcccg tttttgcatc acggcggtta ttacgttctt
     1381 cgtcttcttc tcgggctggc cctccaggtg gaattcactc agaatgtgaa ccacggccac
     1441 tccctgagaa gtgagaggac gaatcttgga aacgcttagc cggatctcga cctcaccgta
     1501 gttcccagcc acggagcttg tgctctcgtc gtagagcctc tggtggatgc gggcgttgtc
     1561 cttccgtctc tgattcggta agaacatgcc gtttatggcg atgtagtcgt gctcctgggc
     1621 aaaagcggag gcgaatagct cgccatcttt acgattctgg ccgtcttgca tgttctgcac
     1681 gagctgtctg atggccgcct cgtcttcccg tcgaatgacc gtggtgttca tcgtccactc
     1741 ccttttcctt caacaacgta aaggcaggac cctcgagacg gaatcgattc gagtttttgc
     1801 gaaaggtggt ggaatgccgg tagcactcta ggataatgta ggggattgat gtgagcgctg
     1861 ttacgagaag gttgcacgac cgatgatcac gatccccctc accaccgaag acctcaccaa
     1921 ggtacgcctc gcccccagcc cgctctggga gacggttacg agcttcgggg tgctgcttca
     1981 ccagggacgc gacacggtgc atgcgccgtg ggccgcccgc gcgcggaggg tcctgcccgg
     2041 cacagacctc tcggcgctgg tcgcggcgat gtgcgtggcg ggccgctgcc cggacttcct
     2101 gaccccgcca cccgacgcct cggccaccac cttcggggag gaactcgaac gcctgaggac
     2161 cacgccttca gaggtcgtcc acaaggaggt agaggcgttc gtgcgggtag aggaggagca
     2221 attcgcttcc ctcgcccccg aaaaggagcg aatgctcaaa gcctacctga aggatcccga
     2281 ggactccctg aggcggctgg tggatgcgct ggtccgctac cacgacgcgg cgttcgcccc
     2341 ttactggccc cggatccggg agcacctgga aggggacacc ctcaggcgcg ggcaggcgct
     2401 cgccctcggt ggcgtcgaag ccctcctctc cggtctgcac ccgaaggcac gctacagcgg
     2461 gggcgtcctg gagctggaca aggcctacga ggcgatcatc gaacccgcgg gacgcgggat
     2521 caccctggtg ccgtccgtct tcgcctggcc ccgcgtcgag gtgctcgtcc aacccggata
     2581 caggcccacc ctggcctacg ggcccagagg ggcggctaag ctgtggtcct cctcgtcgcc
     2641 cgcgaccaac gggacggcgc tggaggccgc tctcggcgcc gggcgggctt cggtcctcaa
     2701 gggcctcctc cccgtgccga gcacaaccac cgagctcgcc cagcaactcc acctgagccc
     2761 ggcctcggtc tcggcccacc tctccaggct caaggccgca gagcttgtcg agccgcaccg
     2821 cagcggtagg agggtctact accgtctaag ctgcgccggc gagtcattgc taggtatatt
     2881 cggcgagacc ggctgaagac caagcgctcc cgataggacg aagccgcggc gacgtgtccc
     2941 tcgtggggcg ctttattcga cgacccttat tgcggtccgc ctgacacgcc gcttaccact
     3001 atggagatca cgctgaggac cacggcgacg atccctagaa tctgcccggt ccggttcgcg
     3061 ccctgcgggc cgaagtagta gccaaggccg ccgaggatga tgccggggaa ctcgatggag
     3121 acgccgttga tcacaaagag gccgagcact gcgcaaaaga cgccgagaac gccaagcgct
     3181 ataccgaccg tcgcctgcaa tcgttactcg cggccttccg gggtaaggcg gtcgatccac
     3241 tcgtcggctt cggcgcgggt catgtccttg agcggcttgc cgagacgatt ctccaggcgc
     3301 tcgacaccgt tctcgccccg ccactcgact gccagcgtct tcagaaggtc gacctggctc
     3361 ttgcgcgcct tccccgcacc gcctctcttc gaggaggctg gtttctccgg ctggccattc
     3421 ggggtgtcct gctctgcagc agcactccgg gaagagtctt gagaagagtt ctgtctcccc
     3481 ccgcggatgg gagtcggcct gcctcgcgag ggcgagctct gggagaccgg aggggcgtcg
     3541 tcgccatcgg agagctcttc gagggcggtc gcgccgacgt tcacagcgtc gcgcagggca
     3601 cgggctttcg ccctggtctc ggccatccgg atcaggtgcg gggcgatgtt gcgcccgacg
     3661 ttctcgggac tcgcatcccc gatgcctgaa aaacgcttgc cgtcctccat ctccgccgta
     3721 gccttcacta tggcgacgtt cccgttcgac tcgtcgggga cctggatcag gtccgtgtct
     3781 atccccgtca gtcccaggct atgggcttcg tcgaggaggc ccgagtagag gacatattgc
     3841 tttccctgac gggtaatcat gaactcttct ctcacaggcc ccgccttact tcggcttcgc
     3901 ggtagtcctc gccttcgagg gagatccaat cgcacatggc gatgatcctg ctcgcagtcc
     3961 tctccccgag ctgggccgcc aggtccttcg gtcccgtgtt agaggtgaag atggtcggca
     4021 gctcctccct gtaacggtgg ttgacgatga cgaagatgcg ctcgcgcacc cactcgttcg
     4081 ccctctcggc gccgagatcg tcgagcagca agagatcagc gttctttact gagtccatcc
     4141 actcgtcgat atcccggcca gggtcgttgt aagcgccgcg catgttgtcc agaagctcgg
     4201 gcaccgtcac gaagagcgag ggcacccgct tcttggtgat gatctcattc atgacggcta
     4261 ccgcgaggtg cgtctttccc acgcccacag ggccacagaa gtacagacct cgcccaccct
     4321 cctgattggc tttccagccc atgatgtagt ccatgacctt ggcaaaagcc ttctgggagt
     4381 tcctgctaaa cccgggatcg aagttgtcga gcgcgtagcc tttcatgcgc ttgctgagac
     4441 cgctgcggtc cctgagggtc gcgacccgcg cgtcgcggcg catcaactcc cactcgcgct
     4501 gctcgttctt cttctcgcac tccggggtgc aaggatggta gtaccactcc ccaggcttgc
     4561 cgtacttctt ctggagggcc ggcgggaact cgtcccactc ggcctggagc tcgcgcccgc
     4621 agtgggggca gatcttatcg tcgagcctca gtacctcggc cccggcatcg cgcttactcc
     4681 gtctccccga agagccactc atagccctcc cggcgtctgg cagcagactg tctatccggt
     4741 ccaccactca ccccctctcc ggcttcacgt atcctcaagg cgcctcccct cacggccccg
     4801 ctctgctcga cgagcctgag cttgcggtcc tgctcgaagc gctccacgtc ctcaggcgtc
     4861 ttcacgcccc gctcgaccca ccgctccagg atgctccgca cgtagccgac cctgcgcgca
     4921 tcgcgctcgc tggctatctt caaagcctcc ctcacggcgt cgggcgtaag cccgtcccgc
     4981 ccgcagtaac cgttcaagaa ctccaggatg tgcggcgctg gcaatgtccc catgaaacgg
     5041 aaataatcgt cggccgaaac cccctctggc tcggccctta tcacctcgac gtcgcggacg
     5101 ggaagctcgt cttcgctggg ccgctcgctc gtttgtgccg aaggcgggtg ctcggcgacc
     5161 tcgagccagc cgtcccttcg gacgacgaag cgggcttcga tcaaggcctc cagagatgcc
     5221 tcgacgccct ccggcgtgga ccgcaacaac tctgccagct cttcgatctc gggagcatcg
     5281 ttcccgagtt cggggaggat acggagcagc tcgtagagcg cgactgcctc ggccccgagg
     5341 tgcggcatcc agcggacgta aagacgggcg cggcctcccc gcgccctatt ctcccccgga
     5401 ggtgtgagcc gaactctctc catcctgcag ccagtatagc ggccagtggc tacgctctct
     5461 acatctgttt gtcgtttcag catgtcagcc ggtcagcact tcagtatttc agcttgtagc
     5521 gtgtgcgagt cctggctgat gagccgcggc gtgctgaccg gctgacatgc taggaagggc
     5581 acggtctgag ggtgctatgc cgggaacgag gccttgact
//
LOCUS       DATMJA010000230         3907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_208473, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000230 DATMJA010000000
VERSION     DATMJA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208473"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..766
                     /locus_tag="VNT12_05450"
                     /pseudo
     CDS             80..766
                     /locus_tag="VNT12_05450"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
     gene            complement(959..1936)
                     /locus_tag="VNT12_05455"
     CDS             complement(959..1936)
                     /locus_tag="VNT12_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWI45600.1"
                     /translation="MTGLIGLAVLALVVFLVAARAVRIIPQSRVAIVQRFGRYHRTAQ
                     SGLTLVVPIMDRMLPKTDLREQVVAFQPQAVITSDNVGMQISTVVYYQVVDARAAEYE
                     VANFHLALEQITQTTLRNVIGNLILDKTLTSRDEINAKLRTVLDEVTEKWGIRVTRVE
                     LKEIIPPRDIQQAMEKQMQAERTRRAAILTAEGDKRAAILKSEGEKASAILRAEGDQR
                     AAVLRAEGEAEAYRNVQAAQIEMTGKLFERLERSDLSPESLRYLYLKALPEMAKGPAS
                     KLFVVPSEIQDLAGKLGALAGGASLATEDGPQDEGEQRRIQAPGAGPNG"
     gene            complement(1933..2391)
                     /locus_tag="VNT12_05460"
     CDS             complement(1933..2391)
                     /locus_tag="VNT12_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWI45601.1"
                     /translation="MDWEIIFWVALAALAFVGELLTVSFFLLFFSLGAVVGLVAALLG
                     FGITVQAVGFLAASVLSMVILRPAFLNRLALGDGERYLGHRGITGKSAVVTEEIEAGG
                     KGMVRIGSGEFWTARSLHPDEKIERGAKVRVLDTDGLTALVDTVEKEEKS"
     gene            complement(2673..3068)
                     /locus_tag="VNT12_05465"
     CDS             complement(2673..3068)
                     /locus_tag="VNT12_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI45602.1"
                     /translation="MAGPETPKLMVDVVIPSEAGVVLIRRGSEPFEGHWALPGGFVDV
                     GETVHEAAVREAAEETGLAVEVSRLVGVYSEPDRDPRGHNVSVAFLARVLGGQMQAAS
                     DAAEVEVIDPNTVELAFDHSRIIADAMAC"
     gene            3287..3904
                     /locus_tag="VNT12_05470"
     CDS             3287..3904
                     /locus_tag="VNT12_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWI45603.1"
                     /translation="MKILCISDRVERMLYGPNLTSYARGVEAVISCGDLPFDYLEYIV
                     TFLGVPVYYVLGNHDPGPEGPEYPGGCTPLDGQVVEAEGEGGGVVLSGLSGSPLYSGG
                     PNQYTERHMRRRARTLSVRIRCRGLVGRPKPGIFATHSPPFGLGDQDDMAHVGFESFV
                     NLIDRHEPSLWLHGHVHLYGPDQERVRQKGDTKIVNVYGHRILDV"
BASE COUNT          721 a         1252 c         1204 g          730 t
ORIGIN      
        1 catcgtttat ccgttttgag atagttagtt agtcgagcgt tcatacacaa acagcgcagg
       61 gactcgctcc tacaaacttt acatcccgag cggctacacc gggcaggccg ttcccctgat
      121 cgtcatgctg cacggttgca cccagagccc ggatgacttc gctatgggca cccgcatgaa
      181 cgaattctcc gaggagcaca ccttcctcgt cgcgtatcct gcgcaagcgg gcaacgccaa
      241 catgcagaag tgctggaact ggttccaggc cgcgcaccag aagcgcggtc ggggcgaacc
      301 gtcgctcatt gcgggcatca cgagacaggt ggtagaggag tacaaggtcg ccgaaggcag
      361 ggtgtacgta gccgggatgt cggcaggagg agcgatggcc acgatcatgg gcgcgaccta
      421 tcccgacctc tacgccgcag tcggcgtcca ctcaggtctc gccccgggtg ccgctaatga
      481 tctttcctcc gcgttcaccg ccatgcgaca gggcggtgcg gtcgtcgcac agaaggacgt
      541 tctcagtgca ggatatcagg aggtcttacc gaccataatc tttcatgggg atggcgatac
      601 taccgttcat ccgcgcaacg gtgaccgcct tctggctcat ctaacccgtg gcgggaacgg
      661 ctcgtcaccg cgagtaggga cacgccaggg tcaggtgccc ggcggttatg agtacaccag
      721 gttcacttac cgcgacgcgg agggtcgaga tctcgtggag cggtagagca tgcacggtct
      781 aggacacgcc tggtcgggcg gtagctaccc aggctcgtac acggatccca gagggcccga
      841 tgcctctgcc gagatggtgc cattcttcgg gcagcaacac aaacggtaga gaggctaaga
      901 ccgtgtccgc ccgcgccgga aacctgctct acagctggtt ctcgcgcctg gcggggagct
      961 agccgttcgg gcctgcgcca ggagcctgga tccggcgttg ctcaccttcg tcttgcgggc
     1021 cgtcttcggt ggcgagggag gcgccgccgg caagggctcc gagtttccct gcgaggtcct
     1081 gtatctcgga aggaacgacg aagagcttgc tcgccggtcc cttcgccatc tcggggaggg
     1141 ccttcaggta gaggtagcgg agtgactcag gagagaggtc agagcgttcc agccgctcga
     1201 agagcttgcc cgtcatctcg atctgggcgg cctgtacgtt gcggtaagcc tccgcctcgc
     1261 cctcggcacg aagtacggcc gccctctgat cgccttcggc gcgaaggatc gccgatgcct
     1321 tttctccttc ggatttcagg atggcggccc gcttgtcgcc ttcggcggtc aggatggcgg
     1381 cgcggcgggt ccgctcggcc tgcatctgct tctccatcgc ctgctggata tctctcgggg
     1441 ggatgatctc cttcagctcc acccttgtca cccggatgcc ccatttctcc gttacctcgt
     1501 cgaggaccgt gcgcagcttc gcattgatct catcacggga ggtcagggtc ttgtcgagga
     1561 tgaggttacc gatgacgttc cgtaaggttg tctgggtgat ctgctccagc gccaggtgaa
     1621 agtttgcaac ctcatactct gctgcccgtg catcgacgac ctggtaatag acgacggtcg
     1681 agatctgcat cccgacgttg tcgctggtga taaccgcctg cggctggaag gcaacgacct
     1741 gttccctcag gtccgtcttg ggtagcatgc ggtccattat cgggacgacg agggtcagcc
     1801 cgctctgagc ggtgcggtgg taacggccga agcgttgcac gatcgcaacc ctgctctgag
     1861 ggatgatcct gacggccctc gccgccacca gaaagaccac tagcgccagc actgccaacc
     1921 ctatgaggcc ggtcatgact tttcctcctt ctccacggta tcgaccaggg cggtaagccc
     1981 gtctgtgtcc agcaccctca ctttggcgcc tcgctctatc ttctcgtcgg gatgcaacga
     2041 gcgggccgtc cagaactccc cgctgcctat ccgcaccatg cccttgcctc ctgcctctat
     2101 ctcctcagtc accacagcgc tcttgccagt gatcccccgg tgtccgaggt acctctctcc
     2161 atccccgagc gcgagccggt tcaagaaggc gggacgcagg atgaccatgc tgagaaccga
     2221 ggccgcgagg aaccctacgg cctggaccgt gatgccgaat ccaagaaggg cggccacgag
     2281 ccccacgaca gcgccgagcg agaagaagag caggaagaaa gagacggtca gaagctcacc
     2341 gacgaaggca agggccgcca gcgcaaccca gaaaatgatc tcccaatcca tgtctcgtgc
     2401 ctcctggcgt aagtttacac caccgccgca agcgcggacc caacctgccc ctatcccacc
     2461 ggcctacaat acgaccgtga ccatcgcata ggacaggggt cgcgccgcag taagcactac
     2521 tgtcctccca cactgtggag ccaggcgaga tcccgacagg agatcatgga ccaagcacgc
     2581 gtagtcctcg actgcgacac cgcgaacgag gttgggtaga tgttgtctgg gacctcgacg
     2641 aacgacgagt actcgccggc ctcatggaag ctctagcacg ccatcgcgtc ggcgatgatg
     2701 cggctgtggt cgaaggccag ttcgacggtg ttggggtcga tgacttcgac ctcggcggcg
     2761 tcagaggcgg cctgcatctg gccgcccagg acgcgggcca ggaaggcgac gctgacattg
     2821 tggccgcgcg ggtcgcggtc tggctcggag taaacgccga cgaggcgcga gacctctacg
     2881 gcgaggcccg tctcctccgc cgcctcgcgc acggcggcct catggaccgt ctcccccacg
     2941 tccacaaaac ctcctggcag cgcccagtgc ccctcgaaag gttcgctgcc acggcggatc
     3001 aagaccaccc ccgcctcaga ggggatcacc acgtcgacca tcagcttcgg ggtctccggt
     3061 cctgccaagg ttcgtacctc ctgcgtgtcc ggcgcatcga aaggttacgc cgtgtcgtgg
     3121 cggggtgcat tatgttggtt cgtgccgcca ggggcaaggg attggaccac ggcccgcatc
     3181 cgggccgcgt ggccccacac gggggcggcc ccggcaccgg tcgcgtcgat gaccggggat
     3241 cccttgcgaa aaagggtatg ctctgcagag tgacaggaag atacacatga agatcctctg
     3301 catcagcgac cgggtggaga ggatgctgta cggtccgaac cttacctcct atgccagggg
     3361 agtcgaagcg gtgatctctt gcggggacct gcctttcgat tacctcgaat acatcgtcac
     3421 ctttctcggg gtccccgtct actacgtgct tggaaaccac gatccaggcc ccgaaggccc
     3481 cgagtacccc ggtggctgca cgccgcttga cggtcaggtt gtggaggcgg agggcgaagg
     3541 cggcggtgtg gtactctcgg ggctctctgg gtctcccctt tactcgggcg gtcccaacca
     3601 gtataccgag cgccacatgc gccgcagggc gaggacgctc tcggtcagaa tccgttgccg
     3661 gggccttgtc ggccgtccca agcccgggat cttcgcgacc cactccccgc ctttcgggct
     3721 tggggaccag gatgatatgg cccacgtagg cttcgagtcc tttgtaaacc tcatcgacag
     3781 gcatgaacca tctttgtggc tgcacggaca cgtacacctt tatgggccgg atcaggagcg
     3841 agtccgacag aagggagaca ccaagatcgt caatgtctac ggccaccgaa tactcgacgt
     3901 gtaactt
//
LOCUS       DATMJA010000231         3339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_563777, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000231 DATMJA010000000
VERSION     DATMJA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563777"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1174)
                     /locus_tag="VNT12_05475"
                     /pseudo
     CDS             complement(81..1174)
                     /locus_tag="VNT12_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1486..3159
                     /locus_tag="VNT12_05480"
     CDS             1486..3159
                     /locus_tag="VNT12_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012413395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWI45604.1"
                     /translation="MCLHPRPVDPVPEETVRVAKAAFPKGTTYMTMRDELGAIFEDKD
                     FLRLFPSRGQPAMAPWRLALVTIMQFAEGLSDRQAADAVRARIDCKYALSLELSDPGF
                     DASVLCEFRGRLLKGGAERLLFEHLLDRFREMGLVKARGKQRTDSTHILAVLRGLNRL
                     ELVGETMRRTLDVLSTVAPEWLRHHVRPEWVGRYVRRLDDHRLPKGKEARRAKAESIG
                     DDGYELLDTILFGDAPCWLREVPAVQTLRRVWVQNFVREQDGSVRWRTSEEGIPPAAK
                     YINHPIDTDARYGRKSTTASWVGYRVHLTETCEEGAPSIITDVQTTPAPVADGDATPI
                     IHQTLKEKDLLPETQFVDTGYLDAELLAQSKRNYGVDLFGPTRQDHRGKARAKHGFGA
                     QDFEIDWEREKAICPEGHESIGWAPLVDNRGGDVIRIKFSARDCGPCRSRELCTLSNA
                     KYPRRNITVHPKEQYEALQAARQREGTAEFKAQYSKRAGIEGTISRSVRTCEARRSRY
                     VGLAKTHAQHLLTATSLNFLRVGEWLADAPRASTRRSPFARLMTGAAAA"
BASE COUNT          601 a         1111 c         1044 g          583 t
ORIGIN      
        1 cagcacgcgg tagacctccc tagcgacgta gcgacgcccc tatattcaag tgacgccggt
       61 cacagtgacg acgcattcct ctaccgtggc gaatcggcga agtcgacgct cgttgtcagc
      121 acgccataga cctcgcgagc cacgtagcgt ttcaggcacc ttatgatctc ctttttggag
      181 ttgccctcgg aggcgcgcct cgcggcgtag cgacgggtgc gctggtcgat gcgcatgcgc
      241 accacgcaga tgaggtgaag ggcgtggtta gcgtctcggt tgcctacttt aggttgagac
      301 gatgccgcac caccttgcca gaggaggctt ccacaggtga cactccgcag aggctggcga
      361 acgacgcctc gctgcccaat ctctcggggt tgtcccccgc gaccattagg agcgtcgcgg
      421 cgtggtccgt gccgatggcc gggagggagg taagctcggg agcggtctcg gctaccagac
      481 gatcgagctg ggcgtcgaga caggcgatct cctcggagag ggcttggtaa cggcgggcga
      541 ctgagcgcag ggcgaacctg gtcgcttcct cctcctcgcc cgggttccca ccggggcgaa
      601 aacgggcggc cacggcgacg agttccttcg tcgagaaacc acgcaggcgc cgccgcagcg
      661 cctccggagc cgtcacccgc acgcctcgga gctggtttgc agcctgggta cgggccttca
      721 ccgcggaacg tctcgcggcc ctcagcaccc ggatcatctc cacgcgaccg ttcccgctct
      781 tgggctcacc ggcggtctcc ccggccagca tcgtgcgtgc tgcggcctcg gcgtctcggg
      841 agtcggactt ccccttgcgc cgaaggtgtc ggcgcttcgg ccgctcgacc tccaataccc
      901 ttatcccgat ggctctcagg tggcgggcta accctgcgcc gtagctgctg gtgccttcca
      961 cgcctgcgcc tctcacccgg ccgaagcctt ccgcccaaca gatgagggcc tcatagccct
     1021 tctcggtcgt cggcacggcc agctctccca gccgcctgcc caggccgtcc aaggcaaccg
     1081 ccacatgcac gtcgaggtgc gtgtcgacgc ccagcaccac ctcctgggcc acatcgcccg
     1141 gattctcgcc gcaacgcttc tccctgccga ccatactcac gcccatctct ccttcccgtc
     1201 tcgcggtcac agccgacccg aagatccgga ggacggacga tactgtgacg ggttacacca
     1261 ccggcgagcc cctatgcggt cacgacgctc cggttctgcg ggtggctacg ggacgacgcc
     1321 cgaggaagac cgacgggtcg ctaccaaggc actttcggct cagcttgagg ctgggtcagg
     1381 cccaccgagg agcgtcgtcc gcaaccaccc atgatgctgc tggcgaattc accagggtcg
     1441 gcacatactt atgggaagcc gtaagtccac cctcctggag agatcatgtg cctgcacccc
     1501 cgtcccgtag atccggtacc agaagagact gtccgcgtcg caaaggccgc cttcccgaag
     1561 ggaacgacct acatgaccat gagagacgaa ctgggggcca tcttcgagga caaggatttc
     1621 ctccgcctgt tcccttcgcg cgggcagccg gcgatggccc cctggagact ggccctggtg
     1681 accatcatgc agttcgccga agggctctcc gatcggcagg ccgcggacgc ggtgagggcc
     1741 aggatcgact gcaagtacgc gctctctttg gagctttctg accccgggtt cgacgcctcg
     1801 gtcctgtgcg agttccgcgg ccgcctgctt aagggcgggg ccgagaggct gctcttcgag
     1861 cacctactcg accgcttccg tgagatggga ctcgtcaagg cgcgcggcaa gcagcgcacc
     1921 gactccaccc acatcctggc cgtcctcagg ggcctcaatc gcctggaact ggtcggggag
     1981 accatgcgcc gcactctgga tgtgctctcc accgtcgcac cggaatggtt gcggcaccac
     2041 gttcgtcccg agtgggtagg gcgttacgta cgcaggctcg atgaccaccg cttacccaag
     2101 ggcaaggaag cccgccgggc caaagccgaa agtataggag acgacggtta tgagctcttg
     2161 gacactattc tttttggaga tgcaccatgc tggctgcgcg aggttccggc agtgcagacg
     2221 ctgcggcgag tgtgggtgca gaactttgtt cgcgagcagg acggtagcgt gcggtggcgc
     2281 acctccgagg aaggcatccc gcccgcagcg aagtacataa accatcccat agatactgac
     2341 gcccgctacg gacggaagtc caccaccgcc tcctgggtgg gctaccgggt gcatctaacc
     2401 gaaacttgtg aggagggtgc gcctagcatc atcaccgacg tgcagaccac ccccgctccg
     2461 gtggccgacg gcgatgccac cccgatcatc caccaaacgc tgaaagagaa ggacctgctt
     2521 ccggagaccc agtttgtgga cactggctac ctggacgccg agcttttggc ccagagcaag
     2581 cgaaactacg gggtggatct gtttggacca acacgacagg accaccgtgg gaaggctcgc
     2641 gccaagcacg gttttggcgc gcaggacttc gagatcgatt gggagcgcga gaaggccatt
     2701 tgccccgagg gccacgagag catcgggtgg gcgcccttgg tggacaaccg cggcggcgat
     2761 gtcatacgca taaagttctc cgccagagat tgcggtccct gtcggagtcg cgagctatgc
     2821 accctctcca atgccaagta cccgcgccgg aacatcaccg tacaccccaa agagcaatac
     2881 gaggctctgc aagcagcgcg ccagcgggaa gggacagccg agttcaaggc ccagtactcc
     2941 aagcgggccg gtattgaagg cacgatctcg cggtcggtga ggacctgcga ggcgcgtcgc
     3001 tccaggtatg tcggattggc gaagacccac gcccagcatc tattaaccgc aacttctctg
     3061 aacttcttac gagtcgggga gtggctagcc gatgccccga gggctagcac ccgtcgctcg
     3121 ccgttcgccc ggctcatgac tggtgcagct gcggcctgat gaattcgcca gcagcatcac
     3181 aggtggttgc ggacggtgcc tcgagccggc ctgacccggg actttggtgg accataggtg
     3241 ccttgaaatg gatccgtcgg ttggtctctg gcgccgtcct gcgactgtcc gtaagaccgg
     3301 agtgtcgtga cctcatagga gcccgctctg agctgaccc
//
LOCUS       DATMJA010000232         3203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_741925, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000232 DATMJA010000000
VERSION     DATMJA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741925"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VNT12_05485"
     CDS             <1..1287
                     /locus_tag="VNT12_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45605.1"
                     /translation="PSGLQDMLQFPLVEALFGRRARRFSLGASLPDGPLAFTSRHDPL
                     PLSELEQMLVLTAAAGNTGWHYMIMRHARYAPHLSNYSGAAGGRTFPSAAGFHTSEVF
                     FTDDEGVYLFETRDAPALIERTTDGPPDLDAILDAHRGRIRKLSDRRLYIPAEEPYME
                     GHNTWCANRPGSTLLIPVGDLAQHMIAVLCFMVQNGYGMYDDVNGEQIPGLERFSHLV
                     DLEEPLPLTFMETYSITECTAELSTSCYSGMLMLQAMGLGGWMFDGIDRYTVLGASGD
                     PEVPGLGFRYDTDERWPLPNPTGLSGVFAGYCPPHYPDMRAAVEAFAERKFGPGGPFN
                     PDTPGPWKESTRVRESAQVHSEEFKECVAIMAQYIYDRFGKFPGTVPSIFILTYLQAH
                     HLELEFYDHHFGPGAYLQTHADHMARWHGEGETSPE"
     gene            complement(1499..2452)
                     /locus_tag="VNT12_05490"
     CDS             complement(1499..2452)
                     /locus_tag="VNT12_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI45606.1"
                     /translation="MRVLLTGGAGFIGSYVAEHLLQSGHEVAVVDDLSRGTRENVPKG
                     AEFYEADIRSGCAEIFRDFEPEALCHQAAQMDVRRSVREPDFDAEINVLGTVRLLQNC
                     TEHAVQKVVFASTGGAVYGEQQVLPAPEDHPLYPVSPYGVSKLACERYLHYYNVQFGL
                     PYVALRYANVYGPRQDPHGEAGVVAIFCGNLTSGKTSTINGGGEQTRDYVYVNDVARA
                     NVLALENEVPSGAYNVGTGVETSVNRLYDQLLEISAKDLPPKHGPSKPGEQLRSCVDP
                     GLAGRTFDWHAQTAIHTGLEETLHSFATPHRSEHSSNERTG"
     gene            complement(2454..>3203)
                     /locus_tag="VNT12_05495"
     CDS             complement(2454..>3203)
                     /locus_tag="VNT12_05495"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWI45607.1"
                     /translation="SPADFERIPVRILEVGSLGTQNLLDLALAKGARFMLASTSEVYG
                     DPLVHPQHEEYRGNVSTTGPRSVYDEAKRYAEALTMAYRRHNRVDTRIVRIFNSYGPK
                     MREDDGRMVPNFIRQALLGEPLTVYGEGTQTRSVQYVEDLIEGVIRLMDSEETRPVNI
                     GNPVEYSVREIAEMVIELSDSQSEISHQPLPQDDPRKRCPDITRAREALGWEPQTPAR
                     EGLAKTLTWFASQAQARTPPKASHTTRGQGA"
BASE COUNT          545 a          965 c         1042 g          651 t
ORIGIN      
        1 ccttcaggcc tgcaagatat gctgcagttt ccgttggtcg aagcgctgtt cggaaggcga
       61 gcacgccggt tctctttagg cgcctcactc cccgacgggc cgctggcgtt cacctccagg
      121 catgatcctc tgccgctctc cgagctggag cagatgcttg tcctgacggc cgcggccggc
      181 aacaccggct ggcactacat gatcatgcgc cacgcacggt acgctcccca tctttcgaac
      241 tactctggag ccgccggcgg ccgtaccttc ccgtctgcgg ctggtttcca caccagcgag
      301 gtgttcttca ccgatgacga gggtgtctac ctcttcgaga cccgcgacgc cccagcgctg
      361 atcgagcgga cgacggatgg gcctcccgac ctcgacgcga tactggatgc ccaccggggc
      421 cggatacgca agctttccga ccgcaggtta tacattcctg cagaagagcc ctatatggag
      481 gggcacaaca cctggtgcgc gaaccgtcct gggagcacac tgctcatccc tgtgggggac
      541 ttagcccagc acatgatagc cgtgctctgc tttatggttc agaatggata cgggatgtac
      601 gacgacgtca acggggagca gatcccggga ctcgagcgct tctctcacct cgtcgacctg
      661 gaggagcccc tgccactgac ctttatggag acctactcga tcaccgagtg caccgccgag
      721 ctctcgacct cctgctactc cggcatgttg atgctgcagg ccatggggct ggggggctgg
      781 atgttcgacg gcatcgaccg ctacaccgtt ctcggggcca gcggcgatcc ggaggtgcct
      841 ggactgggct ttcgctacga caccgacgag cgctggccgc tgcctaaccc tacgggctta
      901 tcgggagtct tcgcgggcta ctgcccgccg cactatcccg acatgcgggc cgccgtcgaa
      961 gccttcgccg agcggaagtt cggtccggga ggaccgttca acccggacac cccgggtccc
     1021 tggaaggaga gcaccagggt gcgggagagc gctcaggtcc acagcgagga gttcaaggag
     1081 tgcgtcgcga tcatggctca gtacatctac gatcgcttcg ggaagttccc cggaaccgtc
     1141 ccgagcatct tcatcctgac ctacttgcag gctcaccacc tggaactgga gttctacgac
     1201 caccacttcg gaccgggggc gtatttgcaa acccacgccg accacatggc ccgctggcat
     1261 ggggaaggtg agacgtcacc cgaataacca tgtctgggga gaccctggcc ggcgggcagt
     1321 agatgcatcg gcgaaggtac gaggaatatg ccgccacgct cagctaggcg tgggtttcat
     1381 cacctcagag cattggtgga gatcgaggtg ggaagaaggc gctgtgcgtt acagggtgtc
     1441 ggtgggcagc agcgtaaacg cggcctccct gcaacagggg atggccgaga caccgggctc
     1501 agccggtgcg ttcgttcgag gagtgctcag atctatgggg ggtcgcgaag gagtgaaggg
     1561 tttcttcgag gcccgtgtgg atggcggttt gcgcgtgcca gtcgaaggta cgtccggcca
     1621 ggccagggtc tacgcagctt cggagctgct cgcccggctt ggacggtccg tgcttaggtg
     1681 gaaggtcttt ggcggagatt tcgagtagct ggtcgtagag gcggtttacg ctggtctcga
     1741 cgcccgtgcc gacgttgtag gcccctgagg gcacttcgtt ttcgagggcc agtacgtttg
     1801 cgcgggcaac gtcattgacg tagacgtagt cgcgggtctg ttccccgccg ccgtttatcg
     1861 ttgaggtctt accactggtc aggttgccac agaagatcgc cacgacccct gcttctccgt
     1921 gtgggtcctg cctgggcccg tagacgttgg cgtagcggag cgcgacgtag ggcaggccga
     1981 actggacgtt gtagtagtgc aggtagcgct cgcacgcgag cttggagacc ccgtaggggg
     2041 agacggggta aagcgggtga tcctcggggg cgggcaatac ctgctgctcg ccgtacaccg
     2101 ctccgcccgt ggaggcgaag acgaccttct gcacggcgtg ctcagtacag ttctgtagga
     2161 ggcggaccgt tccgaggacg ttgatctcgg cgtcgaaatc tggctccctt accgagcgcc
     2221 tcacgtccat ctgggcagcc tggtggcaga gcgcctcggg ctcgaagtct ctgaagatct
     2281 cggcgcaccc cgagcggatg tcggcctcgt agaactccgc tcctttgggg acgttctcgc
     2341 gagtgcctct cgagaggtcg tcgacgacgg cgacttcgtg tccgctctgg agaaggtgct
     2401 ctgccacgta cgagccgata aacccagctc ctccggtcaa gaggacccgc acctcaggcg
     2461 ccttggccac gtgttgtgtg cgaagccttg ggtggcgttc gagcctgtgc ctgagacgcg
     2521 aaccaggtca aggttttggc gaggccttcg cgggcggggg tctgcggttc ccaacccaac
     2581 gcttctctgg cgcgggttat atcgggacaa cgttttctgg gatcgtcctg ggggagaggc
     2641 tggtgggaga tctcgctttg cgagtcagag agttctatga ccatctcggc gatctcacgc
     2701 accgagtact cgacggggtt gcctatgttg accggacggg tctcctcgct atccatcagg
     2761 cggatcacgc cttcgatcag gtcctcgaca tactgcacgc tcctggtctg ggtgccttct
     2821 ccgtagacgg ttagaggctc gcccaggagg gcctgtcgta taaagttagg gaccatcctg
     2881 ccatcatctt ccctcatttt aggaccgtag ctattgaaaa tgcgcacgat tcttgtgtcc
     2941 acccggttgt gccgacggta agccattgtc agggcctcgg cgtaacgctt ggcctcgtcg
     3001 tagacgctcc ttgggcctgt ggtgcttacg ttgcccctgt attcctcgtg ctgcgggtgc
     3061 acgagcgggt cgccgtagac ttcggaggta gaggccagca tgaagcgggc ccccttggct
     3121 agcgcgaggt cgagcaggtt ttgggtaccc aaagagccta cctcgaggat ccttaccggg
     3181 atgcgctcga agtcggcagg gct
//
LOCUS       DATMJA010000233         3420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_860388, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000233 DATMJA010000000
VERSION     DATMJA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860388"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VNT12_05500"
     CDS             <1..474
                     /locus_tag="VNT12_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWI45608.1"
                     /translation="ETPLLTASTPEGARDYLVPARLYPGQFYALPQSPQQFKQLLMVA
                     GFERYFQIARALRDEDQRGDRQPEHTQLDLEMSYTTQDEILDLIEGLYSGIVEQLTQK
                     RLLAKPFPRLTYGEAMDRFGTDKPDIRFGLELHDVSQLAADSEFKVFKGAVESGG"
     gene            475..1176
                     /locus_tag="VNT12_05505"
                     /pseudo
     CDS             475..1176
                     /locus_tag="VNT12_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
     gene            1331..2275
                     /locus_tag="VNT12_05510"
     CDS             1331..2275
                     /locus_tag="VNT12_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWI45609.1"
                     /translation="MRTDYRSPTPVALTFDDGPDPIWTPLVLDALASVEARATFFVVA
                     PLAARYASLISSMRESGHDVAFHCAEHVRHDAMTRREIEADVESGLLALGRSVRYWRT
                     PWGLITPATYEVAIKHRLVGWTADTEDWRGGAPDPAQTVDLEAILARISAGASARDAH
                     PSFPEDPFVRLAPTGALAIPVPDPIGSQGRRASFAEEWRVLRAVAGADGSVGRILDGH
                     FNGVERVSLLAPEPLRSRELEAITAGKLLLGVWGADPIPGEGEPARLVQDEGGARLIG
                     VKTFCSGATGLDRALVLARGPIQRPPLLAYVDLSEGVK"
     gene            2302..2823
                     /locus_tag="VNT12_05515"
     CDS             2302..2823
                     /locus_tag="VNT12_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45610.1"
                     /translation="MRASESHRVVFEGAPLLAVLGEPGELLREPYFSRDAIRTAVTWA
                     GISDTAVDVALDVLAAKAGDGEPDDVVSLAAGRMLAARGTIDRWLAYAADCADADPAS
                     LPGISTQLRESVSRSCREILDEAARAVGSHPFAVSGPLDRARRDLELFLLQHRLEPAL
                     VRRGRQAISDRRP"
     gene            2820..3395
                     /locus_tag="VNT12_05520"
     CDS             2820..3395
                     /locus_tag="VNT12_05520"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWI45611.1"
                     /translation="MKGRLGREYFEELYAESNDPWNFETSEYERNKYGRTLEVLGERR
                     FGRALEAGASIGVFTEMLAGRCDQLLAVDVSERAVVAARERLSDRGHVAVERRTLPEE
                     MPDGPFDLIVASEILYYFTREEMLATLRAFEQELARRGVLLAVHWRRETRTYPLQGDE
                     VHELLLGHTRLHNSKTIVEPDYRLDLFEDPA"
BASE COUNT          639 a         1143 c         1083 g          555 t
ORIGIN      
        1 gaaactcctc tcctcacggc ctcgacaccg gaaggcgcca gggattatct ggtccctgcc
       61 cgcctctatc cgggacagtt ttacgcgctc ccgcagtccc cgcagcaatt caagcagctc
      121 ctgatggtgg ctggcttcga acgctacttc cagatagccc gtgcactgcg cgacgaggat
      181 cagcgcgggg accgccagcc cgaacacacg cagctcgacc tggagatgag ctacacgacg
      241 caggatgaga ttctggacct catcgaaggc ctctactccg ggatcgtcga gcagctcacg
      301 cagaagagat tactcgccaa acccttcccc cgccttacct acggcgaggc gatggaccgc
      361 ttcggtacgg ataagccgga catccgcttc ggcctggagc ttcacgacgt ctcgcagctc
      421 gccgccgatt ctgagttcaa ggtcttcaag ggggcagtag aatcgggcgg ctaggtgcgc
      481 ggcatcgccg tcggcgggct aggggacctg acccgcaggg agctcgacga cctcaccgcg
      541 gtcgcaacct caggaggggc cagaggcctg gccccctttc tggtgcaacc ggatggcctg
      601 aagagtcccg tcgcgaagtt cttcaccgag aagcaacagg caactctgcg cgaatcgctc
      661 ggagccgaag aaggcgactg gttgttcttc gtcgcggaca gaaaccccgt cgtcttcgag
      721 agcctgggcc gcctgcggct ccacttcaga gaccgcctcg ggctagccga ccccgacgtt
      781 ctcggtatgt gctgggtgac ggacttcccg ctcttcgagt ggaacgagga cgaggggcgc
      841 atcgagccga tgcaccacat gttcaccatg ccgcgcgaag acgatctgcc gctgctcgat
      901 accgaccctc tgcaggtcat cggccaactg tacgacctcg tggccaacgg cactgagctc
      961 gcgtcgggga gtatgaggat ccataacccc gacctgcagc agaaggtctt caacgtcatc
     1021 ggtatcggac ctgaggagac gcacaggcgc ttcggaacta tcctcaccgc cttccgctac
     1081 ggcgcgccgc cccacggtgg gatcgcacca ggggtagacc gtttggtgat gctccttacg
     1141 gacgagccga acatccgtga ggtgatggcg ttccccaaga cccaggcagc ccgcgacgag
     1201 atgatggacg cccccggacc cgtctcggaa gagcaactca aggagctgaa catttcgctc
     1261 cgccgccgcc ccgagggcga acgaggctcc gaccccgcag cccggccagg agagggcaac
     1321 aactagagta atgcgtaccg actaccggtc cccgaccccc gtggccctca ccttcgacga
     1381 cgggcccgat cctatctgga ctccccttgt tttggatgcc ctggccagcg tcgaggcacg
     1441 cgccacgttc ttcgtcgtcg ccccactggc tgcacgttac gcttccctca tatccagcat
     1501 gcgggagagt ggccacgacg tcgcctttca ctgcgcagag cacgtccgcc acgacgccat
     1561 gacgaggagg gagatcgaag cagacgtaga gtcaggactc ctggcgctcg ggcggtccgt
     1621 gcggtattgg agaacgccct ggggcctcat cacaccggcg acctacgagg tggcaatcaa
     1681 acaccggctc gtcggctgga ccgctgacac agaggactgg cggggagggg cgccggatcc
     1741 tgcacagacc gtcgacctcg aggccatcct agcgaggatc tccgccggcg cgtcagcgag
     1801 ggacgcgcac ccctcatttc cggaagatcc ctttgtgagg ctagcgccta cgggagcgct
     1861 cgctatcccc gttcccgacc ccatcggaag tcagggacgc cgcgcctcgt tcgcggagga
     1921 gtggcgcgtg ctgcgggcgg tcgccggtgc cgacggctcc gtcggccgca tactcgatgg
     1981 ccacttcaac ggcgtagaac gcgtctcgct cctggccccc gagcccctgc gctcgaggga
     2041 gctggaagcc atcaccgcag ggaaactcct tctcggcgtg tggggcgctg atccgatccc
     2101 aggcgaggga gaaccggctc ggctggtgca agacgagggt ggagcaaggc tcatcggtgt
     2161 caagaccttc tgttccggtg cgacgggcct ggatcgcgcg ctagtccttg cccgtggccc
     2221 catccagaga ccacccctcc ttgcctacgt cgacctctcc gaaggcgtaa agtagatacg
     2281 gcgtggttca aggccgcggg catgcgggca tccgagagcc atcgcgtcgt cttcgaagga
     2341 gccccgctgc tcgctgttct gggagaaccg ggcgagctcc tgagggagcc atacttcagc
     2401 agggatgcaa tccgaacggc cgtcacctgg gccggaatct ccgacacggc cgtcgacgtc
     2461 gcgctcgacg tactggccgc caaagccgga gatggagaac ccgacgacgt cgtctccctc
     2521 gcagcaggca ggatgctcgc ggccaggggc accatcgaca gatggctcgc gtacgccgca
     2581 gactgcgccg acgcggaccc cgcctcccta ccggggattt cgacgcagct gcgtgagtcc
     2641 gtgtcccgct cgtgccggga gatactcgac gaggctgcca gagccgtcgg ctcccaccca
     2701 ttcgccgtct ccggccccct ggaccgcgcg aggcgggatc tcgaactctt cctcctccag
     2761 caccgtctcg agcccgccct ggtacgccgc gggaggcagg ccatctcgga ccggcgacct
     2821 tgaaagggcg actcggacgc gagtacttcg aggaactcta cgcggagtca aatgatccct
     2881 ggaacttcga gacgagcgaa tacgaacgca acaaatacgg gcgaacgcta gaggtcctgg
     2941 gcgaaaggcg gttcgggagg gctctggagg cgggagcttc catcggcgtc tttacagaga
     3001 tgctcgcagg ccggtgcgac cagctacttg cggtagacgt gtcggagcgg gccgtggtcg
     3061 cagcacgcga gcggctctcg gaccgcgggc acgtcgcagt cgagcggcgc acgctaccgg
     3121 aagagatgcc cgacggtccg ttcgacctga tcgtcgcctc ggagatcctg tactacttca
     3181 cgagggaaga gatgctcgcg acgctacggg ccttcgagca agagttggcc cggagaggag
     3241 tcttgctcgc agtccactgg cgcagagaga ccagaaccta cccgctgcag ggtgacgagg
     3301 tgcacgagct actcttggga catacgcggc tccacaacag caagaccatc gtcgaaccgg
     3361 attaccgcct ggatctcttc gaggacccag cgtgaaccat gagggacacc tggtgatcgt
//
LOCUS       DATMJA010000234         5147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_623816, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000234 DATMJA010000000
VERSION     DATMJA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623816"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VNT12_05525"
     CDS             <1..460
                     /locus_tag="VNT12_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45612.1"
                     /translation="EIEARFSETFGRVRDAFGEMVPRMLEGSSGVLNLSEDGVEIGLR
                     LERKGWRPLRVLSGGERALLALSFLFSVFLSRPGGDSGAFCVLDEAEAALDDLNLARF
                     LSVVDSHRANGQYLLVTHQKRTMAAADVLYGVIQDASGATTVVSKRLQGE"
     gene            471..1691
                     /gene="ftsY"
                     /locus_tag="VNT12_05530"
     CDS             471..1691
                     /gene="ftsY"
                     /locus_tag="VNT12_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWI45613.1"
                     /translation="MARSWRSFFRRETDEEQENGPAVASGQERSTEDVEEGNGHLAAD
                     LQTSVEEAAEEVPTAEEVQAERKVPAPEDVWEVDESEPEELLPEPGAVEEAPETIAGR
                     QESGGWFGRLRQGLSRSRASFVGQLNAAVAEFRDVEDEEFWERVEEILISSDVGVPTT
                     AKLVAELEQEALEKNIKGGDELRKLMIEHAARMLEGPVELDVSHTPTVILMVGVNGTG
                     KTTTIGKLSRFLPGTKMFAAGDTFRAAAIEQLQQWGERTDTPVVARERGADAAAVAHE
                     AVHAARSESIDVLLIDTAGRLHTKVNLMAELDKVQRVVQRQLPGAPHEVLMTVDATTG
                     QNGLRQAQMFKEVAGLTGIILTKLDGTAKGGIALAIANEVGVPIKLISVGENVEDLHP
                     FDAREFAEALFGDL"
     gene            1692..3023
                     /gene="ffh"
                     /locus_tag="VNT12_05535"
     CDS             1692..3023
                     /gene="ffh"
                     /locus_tag="VNT12_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWI45614.1"
                     /translation="MFDTLGERLNKALDGVRGSGNLTEDQVKKVTREIRLALLEADVN
                     YGVVKEFVSNVRERATGAEVSKALSPGQQVVKIVDEELANLMGGTAHKLSFASRPPTV
                     VMLAGLNGHGKTTAAGKLALFVRKLGKNPYLVACDTYRPAAIEQLQQIGRELSVPVYT
                     EGTEPKPEEIVERGIREARDGGYDFVIVDTAGRQVVDVEMMEEIRRVRKASKPHSVLL
                     VLDVVTGQNAVEVAEAFQEYADFDGMVLTKLDGDARGGVALSVVSVTGRPIKFASEGE
                     KMGEFDYFYPDRMAGRILGMGDVLTLIEKAEQQMDRTEAEVQGKKLMSGKFTFEDFLQ
                     QMQMIRKMGPLSGLLGMIPGLGKQLKDVEIDDRQLGRVEAMITSMTVQERRNPKLLQN
                     PSRARRIARGSGATQQDVQKLVKQFKEMQKMMAQMGKGGGMPRIPGLSGRR"
     gene            3042..3284
                     /gene="rpsP"
                     /locus_tag="VNT12_05540"
     CDS             3042..3284
                     /gene="rpsP"
                     /locus_tag="VNT12_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWI45615.1"
                     /translation="MAVKIRLARHGSKGNPFYRIVVADSRSPRDGRFIERIGTYDPQQ
                     EPSDIKVDEEKAREWLGKGAQPTDQVRNLLRISGVL"
     gene            3291..3530
                     /locus_tag="VNT12_05545"
     CDS             3291..3530
                     /locus_tag="VNT12_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWI45616.1"
                     /translation="MEQLENLLLVLARSLVDEPEKVEVSGTESDSRVELELRVAPDDM
                     GRVIGRQGRTIRAIRTVVKAASVKAGKRVTVEVLD"
     gene            3536..4033
                     /gene="rimM"
                     /locus_tag="VNT12_05550"
     CDS             3536..4033
                     /gene="rimM"
                     /locus_tag="VNT12_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWI45617.1"
                     /translation="MEELSDPVAIGVVVTPHGVRGTLRVRALGAGKHLREGTQPVVAG
                     VRRRISSARQTPKGFLLDLEGVESRTDAQPLRGEQLFVDRKELDSTEEGEFYVADLVG
                     LTAVGNAGEVLGTVTDTFETAAHEVLVVREEKGRQDLYLPFTMEHVPQVDLQRGRIVV
                     RPPEG"
     gene            4037..4729
                     /gene="trmD"
                     /locus_tag="VNT12_05555"
     CDS             4037..4729
                     /gene="trmD"
                     /locus_tag="VNT12_05555"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWI45618.1"
                     /translation="MRSIDVFCVFPEAVDAAVGVGVVGRAIENGIVGYRSFDLRDYSP
                     DGRIDDMPYGGGAGMVVRVDVVARALEEVYGAPAREVRTGRRVVLTEPAGRRLTQGYV
                     EEVASGPDVTIICGRYGGIDERVWSTLATEAVSLGDFVLSGGEIVAAALADAAIRRLH
                     GVLGNAESLVGESFSAEDTVGPPTYTRPAIWDGEEVPGVLLSGDHAKIRAWREHEALE
                     RTRARTTGRRSP"
     gene            4763..5089
                     /gene="rplS"
                     /locus_tag="VNT12_05560"
     CDS             4763..5089
                     /gene="rplS"
                     /locus_tag="VNT12_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWI45619.1"
                     /translation="MITSDNLQQRDVTFKPGDTIRVHYRVIEGNRERVQIFEGLCISR
                     KGSGIHETFTVRKNSFGVNVERIFPLHSPKIARIEVASRGAVRREKLYYIRDKVGKKA
                     RVKKAR"
BASE COUNT         1038 a         1407 c         1843 g          859 t
ORIGIN      
        1 ggagatagag gcgcgtttct cggagacctt cgggagggta agggacgcgt tcggggagat
       61 ggtgccgcgg atgctcgagg gatcttctgg ggtcctaaat ctctctgagg acggggtgga
      121 gatcgggttg cggctggaga ggaagggctg gcgtcccttg cgcgtcctgt cagggggcga
      181 acgggcgctg ctcgcgctgt cattcctgtt cagcgtgttc ttgagccggc ctggtgggga
      241 ttcgggggcg ttctgcgtgc tcgatgaggc cgaggcggcg ctcgacgatc tcaacctggc
      301 ccgtttcctc tctgtggtag actctcaccg ggctaacggc cagtatctgc tggtcaccca
      361 tcaaaaacga acgatggccg cggcggatgt gctctacggg gtgattcagg atgcctccgg
      421 ggccaccact gttgtgtcga agcgtttgca gggagagtaa gaagtcgcct atggcgcgtt
      481 cgtggcgtag ttttttcaga cgagagacag atgaagagca ggaaaacggc ccggctgtag
      541 ccagcgggca ggagcgctcc accgaagatg tcgaagaagg caacggccac ctcgctgcgg
      601 atctccaaac atccgtagag gaagccgccg aagaggtgcc tacggccgaa gaggtgcagg
      661 ccgagaggaa agtgcctgct cccgaggacg tgtgggaggt cgatgagtcc gagcccgagg
      721 aactcctgcc tgagcccggt gcggtcgaag aggcacctga gacgatcgcc gggcggcagg
      781 agtccggcgg gtggtttggc aggctgaggc agggcctcag ccggagccgc gcctcgtttg
      841 tcgggcagct caacgccgcc gttgcggagt tcagggacgt tgaggacgag gagttctggg
      901 agagagtcga ggagattttg atctcctccg acgtcggggt gccgactacg gcaaagctcg
      961 tcgctgagct ggagcaggag gcgttggaga agaatatcaa aggcggtgac gagttgcgca
     1021 agctcatgat cgagcatgcc gcccgcatgt tagaagggcc tgtcgagctc gacgtctcgc
     1081 atacgcccac ggtgatcctg atggtagggg tgaacgggac gggcaagacg acgaccatag
     1141 gcaagctctc gcgtttcttg ccggggacca agatgttcgc cgccggggac acgttcaggg
     1201 ctgcggcaat cgagcagctc cagcagtggg gcgagaggac ggacacgccc gtggtggcgc
     1261 gagagcgcgg agccgatgcg gctgccgtcg cccacgaagc cgtgcatgcc gccaggagcg
     1321 agagtatcga cgttctgctc atcgacacgg cgggcaggtt gcacaccaag gtcaacttga
     1381 tggccgagct ggataaagta cagagggtgg tacagcggca gcttccgggg gcaccgcacg
     1441 aagtgctgat gacagtcgac gcgaccacgg ggcaaaacgg gttgcggcag gcccagatgt
     1501 tcaaagaggt cgcagggctc accgggatca tcctcaccaa gctcgacggg actgccaagg
     1561 gtggcatcgc cctcgccata gccaacgagg tgggggtgcc gatcaagctg atctcggtcg
     1621 gggagaacgt cgaggacctg cacccgttcg atgcccgcga gttcgcggaa gccctgtttg
     1681 gagatctata gatgttcgat acgttaggcg aaaggctcaa caaagccctc gacggggtcc
     1741 gcggtagcgg caaccttacc gaggaccagg taaagaaggt tacgcgtgag atccggctgg
     1801 cccttctgga ggctgacgtg aactacggcg tagtcaagga gttcgtctcc aacgtccgcg
     1861 agcgggccac gggggccgag gtctccaagg ctcttagtcc tggccagcag gtcgtcaaga
     1921 tagtcgacga ggagcttgct aatctgatgg gcggtaccgc ccacaagctc tccttcgcct
     1981 ccaggccgcc caccgtcgtg atgctcgcag gactcaacgg ccacggcaag acgacggcgg
     2041 ccgggaagct tgcgctgttc gtgcggaagc tcgggaagaa cccgtatctg gtcgcgtgcg
     2101 acacttacag gccggccgct atcgagcagc tgcagcagat cggacgcgag ctctcggtcc
     2161 ctgtgtacac ggagggcacg gagccgaagc cagaagagat cgtcgagcgc ggcatcaggg
     2221 aggccagaga cggcgggtac gactttgtca tcgtcgacac ggccggccgg caggtagtcg
     2281 acgtggagat gatggaggag atccggcgcg tcaggaaggc ctcgaagccg cactccgtcc
     2341 tgctcgtgct cgacgtggta accggccaga acgccgtcga agtggcggag gccttccagg
     2401 agtacgccga cttcgacggg atggtcctga ccaagcttga cggcgacgcc cgaggcgggg
     2461 ttgcgctctc tgtggtctca gttacggggc ggccgatcaa gttcgcctcc gagggcgaga
     2521 agatgggcga gttcgattac ttctacccgg acaggatggc aggccgcatc ctggggatgg
     2581 gcgatgttct cacgctcatc gagaaggccg aacagcagat ggaccgtacc gaggcagagg
     2641 ttcagggcaa gaagctcatg agcggaaagt tcaccttcga ggacttcttg cagcagatgc
     2701 agatgatacg gaagatgggt ccgctatcag gcctcctagg tatgatccct ggtctgggaa
     2761 aacagctcaa ggacgtcgag atagacgaca ggcagctcgg cagagtcgag gccatgataa
     2821 cctcgatgac cgtccaggag cgcaggaacc ccaagttgtt gcagaatccg agccgggcgc
     2881 gtaggattgc gcggggctcg ggtgcgaccc aacaagacgt ccagaagctc gtcaagcagt
     2941 tcaaggagat gcagaagatg atggcccaga tgggcaaggg cggtgggatg ccgcgcatac
     3001 ccggtctgtc gggtcgcagg tagagttcga gaggagatgg catggcggta aagataaggc
     3061 tcgcgaggca tggttccaag ggcaacccct tctacaggat agttgtcgcc gattcacgga
     3121 gccctcgcga cggccggttt atagagcgga tcgggaccta cgatccgcag caggagcctt
     3181 ctgacatcaa ggtcgacgag gagaaggcca gggagtggct cggcaagggg gcgcagccaa
     3241 ccgaccaggt gcgtaacctg ctgaggatct ccggggtcct ctagacggcc gtggaacagc
     3301 tagagaacct attgctcgtt ctcgcccgct cgctcgtgga cgaaccggag aaggtcgaag
     3361 tctcagggac cgagtcggac tcgcgggtgg aactggagtt gagggtagcg cccgatgaca
     3421 tggggcgggt gatcggccgc cagggacgga cgatccgggc gatccgcacg gtcgtgaagg
     3481 ccgcatccgt gaaggcgggg aagcgggtca ccgtggaggt cctggactag agaccgtgga
     3541 ggaactctcc gatcccgtgg cgatcggggt cgtggtcaca ccgcacgggg tccgcggcac
     3601 actgagggtc cgcgccctcg gcgcaggcaa gcacctcagg gaaggcacac aaccggtggt
     3661 cgcaggcgtg agacgccgaa tctcgtctgc caggcaaacc ccgaaaggtt tcttactcga
     3721 cctcgaaggc gtcgagagca ggaccgatgc gcaacccctg agaggcgaac agctctttgt
     3781 cgaccgcaag gagctcgact cgaccgagga gggagagttc tacgtggccg acctggtcgg
     3841 ccttacggcc gtgggcaacg cgggtgaggt cctcggaacg gtgactgata ccttcgagac
     3901 cgccgcccac gaagtgctgg tggttcgtga agagaagggc cggcaggatc tctatctgcc
     3961 ctttaccatg gaacacgtgc ctcaggtgga tctgcagcgg gggcggatcg tggtccgtcc
     4021 gccggaaggc tagtaggtga gaagcataga tgttttctgt gtcttcccgg aagcggtgga
     4081 cgcagccgtc ggtgtcgggg tcgtcggcag ggctatagag aacggcatcg tcggctaccg
     4141 cagcttcgac ctgcgtgact actcaccgga cggccgcatc gacgacatgc catacggcgg
     4201 cggtgccggg atggtcgtac gggtagacgt cgtggccagg gcgctcgagg aggtctacgg
     4261 ggctccggcg agggaggtga gaacggggcg gcgggtcgta cttacggagc cggcgggacg
     4321 gaggctaaca cagggctacg tcgaggaggt ggcctcgggg ccggatgtaa ccatcatctg
     4381 cggacgctac ggcgggatag acgaacgggt ttggagcacc ctcgccacgg aggccgtctc
     4441 gctgggcgac ttcgtgttgt ctggaggtga gatcgtggcc gccgcgctcg ctgatgcggc
     4501 gatccggcgt ctccatggcg tcctcggcaa cgccgagagc ctcgtaggag agtcgttctc
     4561 ggccgaagat acggtcggtc ctccaaccta tacacgccct gccatctggg atggagagga
     4621 ggtgccaggc gtgctattat cgggcgatca cgcaaagatc cgcgcctggc gcgagcacga
     4681 ggcgctggag cgtacgcggg caaggacgac cggaagaagg tcgccttagc catccggaga
     4741 aggtccacga gaggagaaga acatgataac gagcgataac ttgcagcagc gggacgtcac
     4801 gttcaagccg ggcgatacga tacgggttca ctaccgcgtt atcgagggca accgggagcg
     4861 tgtgcagatc ttcgagggcc tgtgtatctc gcgcaagggc agcgggattc acgagacgtt
     4921 cacggtcagg aagaactcct tcggtgtgaa cgtggagagg attttcccgc tccactctcc
     4981 gaagatcgcc aggatcgaag tcgcctcgcg tggcgctgtg aggcgcgaga agctctacta
     5041 catccgcgat aaggtgggca agaaagccag ggtcaagaag gccaggtaag ccagcagcgg
     5101 ccttgtcgaa ggctcgccct accggccttc cgccggagga gtgttct
//
LOCUS       DATMJA010000235         4383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_90805, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000235 DATMJA010000000
VERSION     DATMJA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90805"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VNT12_05565"
     CDS             <1..715
                     /locus_tag="VNT12_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HWI45620.1"
                     /translation="ESIESSLDEVTQSQGIEDRVNEVVQQVQETVGQAAGQAQEAAGG
                     AVQQVQDTAGQATDQAGEAADQATGEAQDLAPGTQLLGETVNDAGQTVQRTVDETGNI
                     IETTLDESGELLDEDITGNLTDLPEAEGFEEYVNEAGSTVRTVQDESGTLIELQLGED
                     GSILDLQIPPSTEETTEEIEESSQQSGGQQDTEEGPNATEAAKQKAEELGVDMSQIEG
                     SGAEGRITVKDVVSASDQG"
     gene            819..1106
                     /locus_tag="VNT12_05570"
     CDS             819..1106
                     /locus_tag="VNT12_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45621.1"
                     /translation="MDDGTRDENGARDTKERLIKIARMYTMCRKAGVAEPMDVAGLAL
                     AAFEDMQLPRAMVFVRSNERNIRDLAWAFENSSSAQEFEQRVKEIKYLSGN"
     gene            1124..1324
                     /locus_tag="VNT12_05575"
                     /pseudo
     CDS             1124..1324
                     /locus_tag="VNT12_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            1727..2062
                     /locus_tag="VNT12_05580"
     CDS             1727..2062
                     /locus_tag="VNT12_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45622.1"
                     /translation="MRRADSVKQEIARKRRNTELLAKEAKRRADQVKRRITVGDVEPR
                     PLPSVREDSLPSIESMESSESMASMESTESAALTGGVPSEEEAYGGGDMGEDVSLGTA
                     PKGAHEEPG"
     gene            2281..2577
                     /locus_tag="VNT12_05585"
     CDS             2281..2577
                     /locus_tag="VNT12_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45623.1"
                     /translation="MFEASNVGWLAPSEDRQMWRLLLARLHPDAGGDHDLFAFACAIR
                     EELSAGVRTTPGVGPGDNVQGIDGPGAPFLKKWQEAMVHWSSANRDALKASWTG"
     gene            complement(2618..2851)
                     /locus_tag="VNT12_05590"
     CDS             complement(2618..2851)
                     /locus_tag="VNT12_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45624.1"
                     /translation="MTQDNQQQQEGQQQQGQGQQQQASPQQLLGRALSDVRKVQNLLE
                     LNYPDQGEAIKMQREAGDLIWAQIQRMQQQDQQ"
     gene            3095..3661
                     /locus_tag="VNT12_05595"
     CDS             3095..3661
                     /locus_tag="VNT12_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HWI45625.1"
                     /translation="MQSGTGVDAGVPDDRGESIGEPDVLLDIPVLNVEEVALQVEDLQ
                     LRVSFKAELADLVKINIGVETEVEGVELQVKGIEAQAQQRGRLDNVRAIFSDVLQAIE
                     HSPQFFRDWSGTDQSTDSSRQTAPDTLGAPDTEESPAAEEADGSEVEATSAAKSKARE
                     LGLDLSSLEGPGSGGRIILRDVQGAAKN"
     gene            3726..4052
                     /locus_tag="VNT12_05600"
     CDS             3726..4052
                     /locus_tag="VNT12_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45626.1"
                     /translation="MANRFFKQTSAEIISELLSDARVKRVREYVQGEEFKADRERVRR
                     DAAERLKNIRERYRAKRRDPETAARERKLTLRLAEVEAEAAELRIQLSVLLEEEEKLR
                     RSLDEL"
BASE COUNT          960 a         1282 c         1462 g          679 t
ORIGIN      
        1 cgagtctatc gagagctcac tcgacgaagt tacccagagc caggggatcg aagacagagt
       61 aaacgaggtg gttcagcagg tacaggagac cgtcggccag gcggccgggc aggcccaaga
      121 ggccgccggc ggggccgtcc agcaggtgca ggacaccgcg gggcaggcca cggatcaggc
      181 cggtgaggcg gcggaccagg cgaccgggga ggcccaagac ctcgcgccgg gcacccagct
      241 cctcggcgag accgtcaacg acgcggggca gaccgtgcaa cgcaccgttg acgagaccgg
      301 gaatatcatc gagaccacac tggacgagag cggcgagctt ctggacgagg acatcaccgg
      361 aaacctcacc gacctgccgg aggccgaagg gttcgaagag tacgtcaatg aagcggggag
      421 cacggtgcgt accgtgcagg acgagtcggg taccctcatc gagctgcagc tcggcgagga
      481 cgggagtatc ctcgacctac agattccccc cagtaccgag gagacgacag aggagatcga
      541 ggagagcagc cagcaaagcg gaggccagca ggataccgag gaagggccca acgccacgga
      601 ggccgccaag cagaaggccg aggagctggg ggtggatatg tcccagatag agggctcagg
      661 tgccgaggga cgcatcaccg taaaggacgt ggtgagcgcc agcgaccagg gctagagtac
      721 tggtaggacg aacctctcct acgagactca taacagcagg gaggggccgg cccgccgggg
      781 ttcggcccct ctccttacaa gggaacgcgg ggagttggat ggacgacggc acgagggacg
      841 agaacggagc gcgagacaca aaggagcggc tcatcaaaat cgctcggatg tataccatgt
      901 gtcggaaagc cggcgtcgcc gagcctatgg acgtcgcggg gctggcgctg gccgccttcg
      961 aggacatgca gctcccccgg gccatggtct tcgtgcgctc caacgagcgg aacataagag
     1021 accttgcctg ggcgttcgag aactcgagct ccgcgcaaga attcgagcag agagtcaagg
     1081 aaattaagta cctctccggt aactgaggag cgcgggggta aacgtgtcga ccagggaccc
     1141 ttacgagtcg ctcggcgtac cgaaaggggc gacgggggac aacatccgcc gagcgtacag
     1201 gcgcctcgcc cgcgagcacc atcctgacgc caaccccgac gatcccgccg ccgaggagcg
     1261 cttcaaggag atccagcggg cttacgagct actctccaac ccggcgaagc gccgggctta
     1321 cgatgatagg tcctcgcgtc tgtccccgcg acgcaaacgg gcgggagcct cgcggacgac
     1381 cgcaggcggg cgcgtgggtc gaaccgccgg ttcgatcaaa ctgtccgact cggtagcgaa
     1441 acgcaccaac gtctcgggaa cccgaaccag cggacacggg aaagtcgagt ggcagctcgg
     1501 cggcgaagac ctggcccgca tctccaaagc cctgggtgtt gacctgactc gcctctcaga
     1561 gctcgtgggc gagcatatac aggtgaacgc caaggtgagc ttcggcgatg cggcgggccc
     1621 gaggccacgc aaacccccga aaaaggggaa gcctcccggt ccctagcgac ctcggaaagg
     1681 tgacgatatg agcatgcgcg agcaagagcg ccggaagcga cgggccatgc gtagggcgga
     1741 ctctgtcaag caagaaatcg ccaggaagag gaggaatacg gagctactgg cgaaggaggc
     1801 caagcgcagg gcggatcagg tcaagcggag gatcacggtg ggcgacgtcg agccgcgccc
     1861 cctaccgagt gtcagggagg actcactgcc gtcgattgag tctatggagt caagcgagtc
     1921 gatggcctcg atggaatcca cggaatcggc cgcgctcacg ggcggggtgc cttctgagga
     1981 agaggcttac ggcggcggag acatgggcga ggacgtgtcg ctcggcacag ctccgaaagg
     2041 cgcgcacgaa gagcctggct agaggatcac cgagcacacg agctccgaga gggacatccg
     2101 cccgcacgcc tagcgcaagg gaggcgagta ggaaggggaa caccaagtgg cgtgctgccg
     2161 ctagccacat gtgggcgcag tgcttcttca cccctctagg tattgcgatc atcccacaac
     2221 taggtttaca gggtctcaac gcccggcata gaatgctcgt tggagaggag gtcttgccca
     2281 ttgttcgagg cgagtaacgt ggggtggctc gcgccatctg aagatcggca gatgtggcgg
     2341 ctgctcctgg cgcggctaca ccctgacgcc ggaggagacc acgacctgtt cgcgttcgcc
     2401 tgcgccatca gggaagagct gtcggctggg gttcgcacta cccctggcgt cgggccgggt
     2461 gacaacgtcc agggcatcga tggccccggc gcccccttcc tcaagaagtg gcaggaggcg
     2521 atggttcact ggtcctctgc caatcgcgac gccctcaagg cctcctggac aggctagggc
     2581 ccaggacgcc ggccatacgt tcgtcccgag gtccgggcta ctgttggtcc tgctgctgca
     2641 tcctctgtat ctgtgcccaa atgagatcac cggcctcgcg ctgcatcttg atcgcctcgc
     2701 cctggtccgg gtagttgagc tcgagtaaat tctggacctt acgaacgtct gacaaggcac
     2761 gccccagcaa ttgctggggg ctcgcttgct gctgttgacc ctgaccttgt tgctgttggc
     2821 cttcttgctg ttgctggttg tcctgcgtca cacgttcctc ccgacgtcgt agcctcgact
     2881 ggaactgagc gcgtaccttc tccgctctga gtacagcctg tgctatctat accccacgtg
     2941 taccgaagct agacacagaa aacacctgcc gcgggtggcc gggtaagttt actttctgta
     3001 cggtaggggc acgaggggct agtgacggac cgttttgtag aggtgggctg gtatgtcatc
     3061 tggagaatcc gaatcgcagc aagatgagca tcctgtgcag agcggcaccg gcgtcgacgc
     3121 tggcgtccct gacgatcgcg gggagagcat aggcgaaccc gatgtgctgc tggacatacc
     3181 ggtgcttaac gtggaggagg tcgccctcca ggtcgaagac ctgcaactaa gggtatcgtt
     3241 caaggccgag ctcgcggatc tcgtcaagat caatatcggg gtcgaaacgg aggtggaagg
     3301 ggtagagctg caggtcaaag gcatcgaggc ccaagcccaa cagaggggcc gcttggacaa
     3361 cgtgcgtgcg atcttcagcg atgtactaca ggccatcgaa cacagtccgc agttcttcag
     3421 ggactggtcg gggacggatc agagcactga ctcttcgcgg caaacggcac cagacaccct
     3481 gggtgcacct gataccgagg aaagtcctgc cgccgaagag gcggacggct cggaagtaga
     3541 ggccacgagc gccgccaaga gtaaggcccg ggaattgggc ttggatcttt catccctcga
     3601 aggcccgggc tccggcggac gcatcattct cagggacgtg cagggggccg cgaagaacta
     3661 gagtcggcga tcgtcctccc gcgccagcgg aggttacaat ggggtcgagc gagaggagga
     3721 agcccgtggc caacaggttt ttcaagcaga cttcggcgga gatcatctcg gagctcctct
     3781 ccgacgcgcg ggtgaaacgc gtcagagagt acgtgcaggg cgaggagttc aaagccgacc
     3841 gcgaacgcgt acgccgcgac gccgcagaac gcctgaagaa catccgcgag cgctaccgcg
     3901 ccaaacggcg cgaccccgaa accgccgccc gcgagagaaa actcaccctg cgcctcgccg
     3961 aggtagaggc cgaggccgcc gagctgcgca tccaactctc cgtgctgctc gaggaagagg
     4021 aaaagctccg aaggtctctc gacgagttgt agctctgccc gcgcaccaag tgataagcag
     4081 cggtatcgac cgtgaatcgc cattcggcta agagcgagaa caaaaagctg acaagctgaa
     4141 gtgctgagaa gcgaaggcga agcctgagcg tgctgaaatg cttgcaaaga ggttcaagac
     4201 ctctttgcaa attcctgaag ccttaccagg tactagtatg caacaggatt gttacccact
     4261 tgtgctctgt tggcaattcg tgtaaaaatg ggcgttccgc accctggaca ccataggaga
     4321 ggacttgttg acccctggag ccggacttta cgaccctatg tacgagcacg atgcttgcgg
     4381 gct
//
LOCUS       DATMJA010000236         4371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_387863, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000236 DATMJA010000000
VERSION     DATMJA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387863"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VNT12_05605"
     CDS             complement(<1..482)
                     /locus_tag="VNT12_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWI45627.1"
                     /translation="MDIYSRLLKDRIIFLGTPVDDQVANAIMAQLLHLESEDPEQDIN
                     LYINSPGGSVTAGLAIYDTMQFVKPDISTTALGMAASMGAFLLAAGAKDKRNALPNTR
                     ILLHQPSVGGLAGQASDVEIHAKELIMTKRRLNEILAENTGQDYERIERDTDRDYIMG
                     P"
     gene            complement(539..1921)
                     /gene="tig"
                     /locus_tag="VNT12_05610"
     CDS             complement(539..1921)
                     /gene="tig"
                     /locus_tag="VNT12_05610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWI45628.1"
                     /translation="MTTNVTKLEENKVRLDIEVAPETVRRGVEAKIRELGQQVRVPGF
                     RPGKAPRRVIENRLGRDYIYMEALQEQLPGWYSQAVVETDVRPIDRPEIHFDDPLDEE
                     EGFKFSATVEVRPEAELGDYKGVEVPRREVEISEEDVEDRIEDMQAQFATLAAVEGRP
                     VQEGDFVILDFKGERLAGGPLEGAEAEDYLLEIGGGELLPDFEQNIVGMNANERRQFG
                     VTFPMDYDEETLRGQSVLFNVHVKEIKERDLPPLDDEFVKEASEFETLDELRVAVREE
                     LEATAERRAEGEFRARVLDVVAEGGEVEVPEVMVHEKAHEMVESFERSISSQGMDPEQ
                     YYELAGTDHAAFEERVMPDAEDTVRKELVLDAVARAEGIVADEDEVMHEIGHLAEDSE
                     RSAEEIVKTLRDNGTYALLEEEISRQKALDFLVENAVPVPMPEDETEEEERVEARAGV
                     MESEEREEQQ"
     gene            complement(1964..2044)
                     /locus_tag="VNT12_05615"
     tRNA            complement(1964..2044)
                     /locus_tag="VNT12_05615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2009..2011),aa:Leu,seq:tag)
     gene            complement(2272..3234)
                     /locus_tag="VNT12_05620"
     CDS             complement(2272..3234)
                     /locus_tag="VNT12_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWI45629.1"
                     /translation="MRDFQAYTKERQGTRRLHWWMWSLPVLLVSLFVGIELVAGPVSI
                     ERLTSASPPDSGKVEAAPSDWVERMFGGQPGLTDGPLNVLVLGVDTRPDSEEMGSRTD
                     TIMLVQVVPKTGDVKLLSVPRDLLVEVEPGERGKINAAYNYGGVDETIDALENYSGVP
                     VDHYAVVDFEGFERVIDAMGGVRVEVGDGQFPEKWRMGEGVQRLNGHKALIYARYRGT
                     PGADLDRMQRQRELVGALRSEALRWHTVKTLPEIMVVMNENVQTDLDLDGAITLGRVL
                     IRKGRHAEMTSQQLQGTPETLSNGEQVLIPDEEANQAILDDFHY"
     gene            complement(3476..4315)
                     /locus_tag="VNT12_05625"
     CDS             complement(3476..4315)
                     /locus_tag="VNT12_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45630.1"
                     /translation="MERGSMQDLFSLAGKVAVVTGATGVLGSEMARSLARSGARVAVL
                     GRREERAYRVAGEIEVAGGESLALPADVLDTGQLQDARDALLEHWGRVDILVNAAGGN
                     VPEATLSDSVGVFQLPEKALRQVMDLNFLGTLLPCQIFGAAMVEGAEEPDGCIVNISS
                     MAASKTLTNVVGYSAAKAAVDNFTRWLAVELARSYGPGLRVNAIAPGFFIGEQNRDLL
                     LDEDGSLSKRGQTIIEHTPAGRFGEPDELGGTLIWLCSPASAFVNGIVVPVDGGFSAF
                     SGV"
BASE COUNT          712 a         1459 c         1268 g          932 t
ORIGIN      
        1 ggacccatta tgtagtcgcg gtcggtgtcg cgctcgatcc tctcgtaatc ctgcccagtg
       61 ttctcggcga ggatctcgtt caggcggcgc ttggtcatga tgagctcttt ggcgtggatc
      121 tccacgtcag aggcctggcc ggccagtccg cctacgctcg gctggtgtag gagtatgcgc
      181 gtgttcggca gggcattcct cttgtccttg gcaccggcgg cgagcagaaa ggctcccatc
      241 gaggcggcca tgccgagggc cgtggtggag atgtcgggct tgacgaactg catggtgtcg
      301 tagatcgcaa gccccgccgt gacgctgcca ccaggggagt tgatgtacag gttgatatcc
      361 tgctccgggt cctcgctctc caggtgcaag agctgcgcca ttatggcgtt ggccacctgg
      421 tcatccacgg gagtcccgag gaagatgatg cggtccttga gcagccgcga gtagatgtcc
      481 atggcccgct ctccacgcgg gctctgctct ataacgtatg ggatgacgtt gtctatgctc
      541 attgttgttc ctctcgttct tcgctctcca tcacgccggc cctggcctct acccgttctt
      601 cttcctccgt ctcatcctcc ggcataggga caggcaccgc gttctcgacc aggaaatcca
      661 gggctttctg gcgtgagatc tcctcctcca gcagcgcgta cgtcccgttg tcgcgaaggg
      721 tcttgacgat ctcctcggcg ctcctttcgg agtcttcggc gaggtgtccg atctcgtgca
      781 ttacctcgtc ttcatccgca actattccct cggccctagc aaccgcgtcg agcactaact
      841 ccttcctaac ggtgtcttcg gcgtctggca tgacccgctc ctcgaaggcg gcgtggtctg
      901 tgcctgcgag ctcgtagtac tgctcagggt ccattccctg cgaacttata ctccgctcga
      961 agctctcgac catctcgtga gccttctcgt gcaccatgac ctcgggaacc tctacctcac
     1021 cgccttcggc gacgacgtcg agcacgcgcg cacggaactc accttccgca cgccgctctg
     1081 cggtggcctc caactcttct cttacggcga cgcgcagttc gtcgagggtc tcgaactcgc
     1141 tcgcctcctt gacgaactcg tcgtcgagag gcggtaggtc gcgctctttg atctccttga
     1201 cgtgcacgtt gaagagcaca gactggcccc tgagcgtctc ctcgtcgtag tccatcggga
     1261 aggtgacacc gaactgcctg cgttcgttgg cgttcatccc gacgatgttc tgctcgaaat
     1321 ccggcaggag ctcgccgccc ccgatctcga gcaagtagtc ctcggcctcg gcgccctcta
     1381 gcggccctcc ggccagcctc tcgcccttga agtccaggat cacgaagtct ccctcctgaa
     1441 ccggcctccc ttcgacggcg gccagggtgg cgaactgggc ctgcatgtcc tcgatcctgt
     1501 cctcgacgtc ctcctccgag atttccacct cgcgcctggg gacctcgacg cccttgtagt
     1561 ccccgagttc ggcctcaggc cgcacctcca cggtggccga gaacttgaag ccctcctcct
     1621 cgtccagcgg atcgtcgaag tggatctccg gccggtctat agggcgcacg tcggtctcga
     1681 cgacggcctg cgaataccag ccagggagtt gctcctggag ggcctccatg tagatgtagt
     1741 cgcgcccgag gcgattctcg atcacccgcc gcggcgcctt gccgggacgg aaaccgggaa
     1801 ccctgacctg ctggccgagt tccctgatct tcgcctcgac accccgcctg accgtctcgg
     1861 gggccacctc gatgtcgagc ctgaccttgt tctcctcgag tttcgttacg ttagtcgtca
     1921 taccctccag ttcctcttga ccagcccgac ctaggcggcg atgtgcgaga ggcgggattt
     1981 gaacccgcac gccgtgaggc actagatcct aaatctagcg tgtctaccat tccaccactc
     2041 tcgctcgccg tagggcttgc gccggattat agcagccacc caatcgcttc ccgtccgtcc
     2101 ctcgaccctt tacgctaagg gcgcaagcgt agcgatctgc tttcagcaga tcgcgctagt
     2161 cagcacgctc aggccttgcg gccttcgctt atcagcattt gagcttgtgt gcctgaaagc
     2221 cctggctgac tggctgagaa ggcggcacag ctgccgtgct gacatgcttc gctaatagtg
     2281 gaagtcgtcg aggatggcct gattggcttc ttcgtctggt atgaggacct gctctccgtt
     2341 ggagagggtc tccggggtac cctggagttg ctgggaggtc atctcggcgt gtcttccctt
     2401 gcggatgagg acccggccca gggtgatggc tccgtcgaga tccaagtcgg tctggacgtt
     2461 ctcgttcatg accaccatga tctcgggcag tgtcttgacg gtgtgccacc gcagggcctc
     2521 gctgcgaagg gcgccaacga gctcccgctg gcgctgcatg cggtcgaggt cggcacctgg
     2581 ggttcctctg tacctggcgt agatcagggc tttgtggccg ttgagcctct gcacaccttc
     2641 gcccatacgc cacttctcgg ggaactggcc gtctcctact tcgaccctga ccccgcccat
     2701 cgcgtcgatg actctctcga aaccctcgaa atccacgacg gcgtagtggt ctacgggtac
     2761 gcccgagtag ttctccagcg cgtcgatcgt ctcatcgacg ccgccgtagt tgtaggcggc
     2821 atttattttg cccctttctc ccggttctac ctcgaccagg agatcccgcg gtacagagag
     2881 cagtttgaca tctcctgtct ttggcaccac ctgcacgagc atgatggtat ccgtgcggct
     2941 gcccatttcc tcgctgtcgg gcctggtgtc cacgccgagc acgagcacgt tgagcggacc
     3001 gtccgtgagc ccgggttgcc caccaaacat cctctcgacc caatcactcg gggcagcctc
     3061 gaccttgccg gaatcaggtg gcgacgcgct ggtgagcctc tcgatcgaga ccgggccggc
     3121 cacgagctcg atgcctacaa agagagagac tagcaggaca ggtaacgacc acatccacca
     3181 gtggaggcgg cgcgtacctt gacgttcctt ggtgtaagcc tggaaatccc tcatccacag
     3241 ggaagtctag cacgctacgg cctttggcct ccgcttgtca gcacggcgct tcgcgccttg
     3301 tcagcacgct acgcctttcg ggctccgctt gtcagccgtc agcttctccc tctccggccc
     3361 cgctgcgcgg cgcatgagga cccaccctac gtggcggtcg ggttctatgc tggtggcggc
     3421 ctcagacgac gtgctgacaa gcgaaggcga agcctgagcg tgctgaaatg ctgatctaca
     3481 ccccgctgaa ggcggaaaaa ccaccgtcta cgggcacgac gattccgttg acgaaggctg
     3541 aggccgggct gcagagccag atcagggtcc cccccaactc atcgggttcg ccgaagcgcc
     3601 ccgccggggt gtgctcgatg atagtctgcc ccctctttga taggctacca tcctcgtcga
     3661 gcaacaggtc cctattctgc tcccctataa agaagcctgg cgctatggcg ttgaccctga
     3721 ggccaggacc gtagctgcgt gcgagctcga cggcgagcca gcgggtgaag ttgtcgacgg
     3781 cggccttggc ggccgagtag ccgaccacgt ttgtcagggt tttggaagcg gccatggaag
     3841 agatgttcac gatacaccca tcgggctcct cggcaccctc gaccatggcg gccccgaaga
     3901 tctggcaggg gagcagggta cccaggaagt tgaggtccat cacctgccgc agggctttct
     3961 caggcaactg gaagacaccg acactatcgc tgagagtggc ctcgggcacg ttaccgccag
     4021 cagcgtttac gaggatatcc acccggcccc agtgctcgag cagagcatct ctggcatctt
     4081 ggagctggcc agtatcgagt acatcggcgg gaagggcgag tgattcccca ccagccactt
     4141 ctatctctcc tgcgacccgg taggccctct cctcgcgacg accgagcaca gccaccctgg
     4201 cgccgctgcg cgcgagggag cgggccatct cagagccgag tacacctgtc gcgccagtga
     4261 cgacggctac cttacccgcg agggagaaga gatcctgcat cgatccccgt tccaccgttc
     4321 tcctccttac gtcctgcgca caggctctct ctggaatccg taggccctct t
//
LOCUS       DATMJA010000237         6336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_446946, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000237 DATMJA010000000
VERSION     DATMJA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446946"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VNT12_05630"
     CDS             complement(<1..732)
                     /locus_tag="VNT12_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45631.1"
                     /translation="MKTESPGVEAATALLRRFEPVIRSTTGDKFYPMDVEPYVRACSL
                     WVQRPGEEAVCVVPDGELSLDRLAQQPMDEAGAVHFLKFTDAEDLGPDSRGRRLRLFR
                     RRAKDRKESRRVFRDGRGRLARVGYFSRFVDALYSIALLARGRVPGESAAAASVAYER
                     IMTEQEQYRYHGRVLRQDGWIVLQYWLFYPFNDWRSGFFGANDHEADWEKIFVYLSES
                     EAGEVTPEWVAYAAHNYTGDNLRRRW"
     gene            complement(722..1120)
                     /locus_tag="VNT12_05635"
     CDS             complement(722..1120)
                     /locus_tag="VNT12_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45632.1"
                     /translation="MRVIGVLLIVEVIGLLVLGGYEFLQVDWRGINLRTPQHQAVEVA
                     VFALFVPSAILTLLSALSFLFLYRRGWLLAAIAQGMSLAVCLWLYSWFQPGYVYPIMA
                     YCILMILYLNSQDVRMVFRPGQSPQASHEN"
     gene            complement(1201..2019)
                     /locus_tag="VNT12_05640"
     CDS             complement(1201..2019)
                     /locus_tag="VNT12_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWI45633.1"
                     /translation="MRLITYSVSGGEQRVGSLEGDEVRPLAHEDMIEFIEYGGSPEPG
                     EDTLPLGDVRIHAPIPRPQKVIGIGLNYEDHAAETGADIPDKPIVFAMYPNAVIGPGE
                     AIRIPPITEQADYEAELAVVIGRAARNVSEAEALDYVFGYTNANDVSSRDLQFSEGGQ
                     WTRSKSIDTFCPLGPFIATTDEIEDPQDLSIRCILNGEIMQDGTTSKMIFSVAELVSF
                     LSQGMTLVPGDVIVTGTPPGVGTARDPQVWLKAGDEVTIEIQGLGSLTNPVESD"
     gene            complement(2091..2726)
                     /gene="pdxH"
                     /locus_tag="VNT12_05645"
     CDS             complement(2091..2726)
                     /gene="pdxH"
                     /locus_tag="VNT12_05645"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HWI45634.1"
                     /translation="MDASSLRKEYTSTGLDKADVDPDPIVQFHEWFEKVIEADLHEPN
                     AMILATATADGKPSARTVLLKGYDERGFVFYTNYEGRKAKEIEANPTCALLFYWGELE
                     RQVRIEGRASRLSGEESDTYFASRPQGSRLGAWASEQSRPVEDRSILEGRVRALEAEY
                     EGREIPRPPFWGGYRVEPDTIEFWQGRESRLHDRLVYHRSGRGWRIVRLQP"
     gene            complement(2734..2895)
                     /locus_tag="VNT12_05650"
     CDS             complement(2734..2895)
                     /locus_tag="VNT12_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45635.1"
                     /translation="MCRRRIEPGQGQESVWDYPRPPRVEAQEGYFYGRWISSDIVGPF
                     KGAPGTWGW"
     gene            3044..4144
                     /locus_tag="VNT12_05655"
     CDS             3044..4144
                     /locus_tag="VNT12_05655"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWI45636.1"
                     /translation="MFSRVGDGLSAGYHRLRTSVWSVVQAAVAASLAYFVAEVLVGHE
                     QPFFAPIAAVICLSVTLGQRNRRAVELVFGVAVGLMVADLLVLVIGNGTLQIAVVVLL
                     AMAAAVFFGGGTMLVNQAAVSAVLVVVLQPPEDVFPPERFVDALIGGGVALAINYLFP
                     INPERLVERAAAPVFDELAAVLEEISAVLESGGEDQAERVLERAREIDGRVRTLNEAL
                     EAGHETARLSPTRRRSLRQLEFYGSAGIRIELAVINTRVLARGVYNAIRRGDSIAPQL
                     PEAVLDLSRSVRALAAFLEDEEGPEEARRWALEAARTATEILEERHELGISVLVGQVR
                     SAAVDLLRSTGMDQASALQALEDAAGSASEIG"
     gene            complement(4163..4555)
                     /locus_tag="VNT12_05660"
     CDS             complement(4163..4555)
                     /locus_tag="VNT12_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWI45637.1"
                     /translation="MKRENVTSGTVWESRVGYSRAVRRGRFVYVSGTTATDGSGGYVG
                     VGDPYAQTVQAINNIRVALQAVGADLSDVVRTRIYVVNIEDWEKIGSAHGECFGKVRP
                     ATSMVEVSRLIAPEILVEIEADALVDEG"
     gene            complement(4602..>6336)
                     /locus_tag="VNT12_05665"
     CDS             complement(4602..>6336)
                     /locus_tag="VNT12_05665"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HWI45638.1"
                     /translation="YASSPEEHFFGFGTQFTYFDMKGRIVPILIREQGIGRGEQPVTW
                     AVDWRAGAGGDPYTSYASVPHYMTSEARSLFLENYEYSTFDLREGDRVQIGVFSSLMR
                     GQILGGDTPAQLIEQYTEYSGRMRPLPAWIISGAVVGLQGGTDRVRRISDELESLGTP
                     IAALWLQDWVGQRKTSFGTQLWWNWELDRDHYPNWGSLREGLEEKNIKLMTYINPFLC
                     DDAAQEKDHRCNLFEEAARSGFLVKKQDGEPYTITISSFSAALVDLTNPEAWNWIKDI
                     IKGELIGNGASGWMADFGEGLPYDAVLFSGADPKSYHDRYAEEWARVNREAISEAARG
                     EDIVFFNRSGYTKSPRYSTLFWVGDQLVDWDEHDGIKSAVTGLLSSGLSGYSLEHSDI
                     GGYTALDYPLLKYHRSKELLLRWIEFGAFTTVFRTHEGNIPEVNHQIYSDRETLTHFT
                     RFAKIYAGWKPYRMDLVKAASETGLPVLRHPFIHYSNDPEVSGLEYQFMVGPELMVAP
                     VLDPGEDSVEVYLPAGKWVHLWTGERYGSPQRGINETVPAPIGEPAVFYKEDSAEGTR
                     FREELERRGLL"
BASE COUNT         1105 a         1995 c         1960 g         1276 t
ORIGIN      
        1 ccacctgcgg cggaggttgt cccctgtgta gttgtgggcg gcgtaggcca cccactccgg
       61 cgtcacctcg cccgcctccg attcagagag gtagacgaag atcttctccc agtcggcctc
      121 gtggtcgttc gcgccgaaga atccgctcct ccagtcgttg aacgggtaga acaaccaata
      181 ctgaagtacg atccagccgt cctggcggag cacacgcccg tggtagcggt attgttcttg
      241 ctcagtcatt atgcgctcgt aggccaccga agccgctgcg gccgattccc ctggcaccct
      301 tccgcgggcc aggagcgcga tcgagtacag ggcgtcgacg aagcgggaga agtacccgac
      361 gcgagcgaga cggccccgcc cgtccctgaa gactctcctc gactccttgc ggtccttggc
      421 gcgtctgcgg aacagtcgca ggcgtctgcc acgagagtca ggcccgagat cctcggcatc
      481 agtgaacttg aggaaatgta cggcgccggc ctcgtccata ggctgttgcg cgagccggtc
      541 caggcttaac tccccatcag gcacgacgca tacggcctcc tcgcccggcc tctgtaccca
      601 gaggctgcag gctcggacgt agggttcgac gtccatcggg tagaacttgt cccctgtggt
      661 cgagcggatc acgggctcga atcgccggag aagggccgtg gcggcttcca cgccggggct
      721 ttcagttttc atgcgacgcc tgaggactct gtccggggcg gaacaccatc ctcacgtcct
      781 gggagttgag gtagaggatc atcagtatgc agtaggccat gatcgggtag acgtaacctg
      841 gctgaaacca ggagtagagc cacaggcaca cagccaggct catgccctgc gcgatcgcgg
      901 ccagaagcca ccccctgcgg tacaggaaga ggaagctcag cgccgagagt agcgtcagga
      961 tggcggaagg gacgaagagg gcgaagaccg ccacttctac tgcctgatgc tgcggagtcc
     1021 gtaggttgat ccccctccag tcgacctgca agaactcgta gccaccgagg acgaggagcc
     1081 cgatcacctc gacgatgagg agcaccccga tcaccctcac aggccggtat gatgtcttct
     1141 ccggttctac cagggtctgg gtgccttccc tcgcctaatc gtcaattcta catcccgcct
     1201 ctagtccgac tcgacggggt tggtgaggga tccgaggccc tgtatctcga tcgtgacctc
     1261 atctcctgcc ttcagccaca cctgcgggtc gcgggccgtg ccgacgccgg gtggcgtgcc
     1321 tgtgactatg acatcgccgg ggacgagggt catgccctgg gagaggaaag agacgagctc
     1381 ggcgacagag aagatcatct tcgatgtcgt cccatcctgc atgatctcgc cgtttaggat
     1441 gcaacggatg gagagatcct gcgggtcttc gatctcgtcc gtggtcgcga tgaaaggccc
     1501 taacgggcag aaggtgtcta tggacttgct gcgcgtccac tggccaccct ccgagaactg
     1561 gaggtcacgc gaggagacgt cgttggcgtt cgtgtagccg aagacgtagt cgagggcctc
     1621 ggcctcggag acgttgcggg ctgccctccc tataacgacg gcgagctcgg cctcgtagtc
     1681 ggcctgctcg gttatggggg gtatcctgat ggcctcgcct ggccctatga ccgcgttcgg
     1741 gtacatggca aagacgatgg gcttatcggg gatatccgct cccgtctcag cagcatggtc
     1801 ctcgtagttg aggcctatgc cgatcacctt ctgaggcctc ggtatcgggg cgtggatgcg
     1861 gacgtcgccg agcggcaacg tatcttcacc gggctccggg ctcccgccgt actcgatgaa
     1921 ctcgatcatg tcctcgtggg cgaggggcct tacctcatcg ccctccagag acccgacgcg
     1981 ctgctcccca ccactcaccg aatacgttat caatctcaag agctctcctc tcctaggatt
     2041 cgagacgcaa cgatacgcat cttagcagca acgctggtgc tcacacgtcc ctacggctgc
     2101 agacgcacta tcctccaccc tctcccggaa cggtggtaga cgagcctgtc gtgcagccta
     2161 ctttcgcgtc cctgccagaa ctcgatggtg tcgggctcga ccctgtagcc cccccagaaa
     2221 ggggggcgtg ggatctcacg cccctcgtac tcagcctcga gtgcccttac ccgcccttct
     2281 agaatgctcc tgtcctcgac gggacggctc tgctcggaag cccacgcccc gagacggctt
     2341 ccttgcggac ggctcgcgaa gtaggtgtcg gattcttcgc ccgagaggcg gctggcgcgg
     2401 ccctcgatcc tgacctgccg ctccagctcg ccccagtaga acagcaaggc gcacgtgggg
     2461 ttcgcctcga tctccttcgc cttgcgtccc tcgtaattgg tgtagaagac gaagccccgc
     2521 tcgtcgtagc ccttgagaag gacggtgcgg gctgagggct ttccgtctgc ggtggcggtg
     2581 gcgaggatca tggcgttcgg ctcgtggaga tcagcttcga tgaccttctc gaaccactcg
     2641 tggaactgga caattggatc ggggtctacg tcggctttgt ccaggcctgt gctggtgtat
     2701 tccttgcgga ggctcgaagc atccacggtg tatctaccag ccccaggtgc cgggagcgcc
     2761 tttgaaaggg ccgactatgt cggaggagat ccaacgccca tagaagtacc cttcctgcgc
     2821 ctcgaccctc ggcggtcgtg ggtaatccca gacggactcc tgtccctgac ctggttctat
     2881 tcgcctgcgg cacatgaagt cctcctcgtc tttgatctcc tgagattata tggtcgagag
     2941 aagtacacgt gtgtacttag gtgtgcgatc cgcgaacacc tagcagtcct cgtgagggtg
     3001 tatagttcac ggggttcgtg cgggtggcgt gagagaagga cgtttgtttt ccagggtcgg
     3061 tgacggtctc tctgcagggt accaccgtct cagaaccagc gtgtggtctg tcgtgcaggc
     3121 ggctgtggcc gcgagtctgg cttacttcgt ggctgaagtg ctcgtcggac atgagcagcc
     3181 cttcttcgcc cccatagccg ccgttatctg cctcagcgta accctcggcc agaggaacag
     3241 gcgcgccgtc gagctcgtct tcggggtcgc cgttgggctc atggtggcag acctcctcgt
     3301 gctcgtcata gggaatggga cgctccagat cgccgtcgtc gtactacttg ctatggcggc
     3361 cgcagttttc ttcggaggag gaaccatgct cgtcaaccag gctgccgtct cggcagtcct
     3421 cgttgtcgtg ctgcagcccc ccgaggacgt cttcccgccg gaacgctttg tcgacgcact
     3481 catcgggggc ggtgtggcgc tcgcgatcaa ctacctcttc cccatcaacc ccgagcgtct
     3541 ggttgaaagg gccgcggctc ccgtcttcga tgagttggcc gctgtgctgg aggagatctc
     3601 cgccgtgctc gaaagcggcg gcgaggatca ggccgaacgg gtcctggaac gggccaggga
     3661 gatagatggc agggtcagaa ccctgaatga ggcgctcgag gccggccacg agacggccag
     3721 gctctccccc acccgtcgcc gctccctgag gcaactcgag ttctacggca gcgccggtat
     3781 aaggatcgag ctggccgtca taaacacccg cgttttggcc cggggcgtct ataacgccat
     3841 caggcgagga gacagtattg cgccacagct acccgaggcc gtgctcgacc tctcgcggtc
     3901 cgtgagggcc ctggccgcgt ttctggagga cgaagaaggg cctgaggagg cccgccggtg
     3961 ggcgctcgag gccgccagga ccgccaccga gatcctggag gagcgccacg agctcggaat
     4021 cagcgtcctc gtcggccagg tccgctccgc cgccgtcgac ctgctaagga gcacggggat
     4081 ggatcaggcc tcagccctgc aggccctgga ggacgccgca ggcagcgcct cggagatcgg
     4141 gtagggatgg tgcagaccgc ccttaccctt cgtcgaccag ggcgtcggct tcgatctcga
     4201 ccaggatctc gggggctatg aggcggctga cctcgaccat gctcgtggcg ggacggactt
     4261 tgccgaagca ctcgccgtgg gcagacccga tcttctccca gtcctcgatg ttgacgacgt
     4321 agatcctggt cctgaccacg tcggagagat ccgcgccgac agcctggagc gccaccctga
     4381 tgttgtttat ggcctggacg gtctgcgcat aggggtcgcc cacacccacg tatcccccgc
     4441 tcccgtccgt ggctgtggtc cccgagacgt acacgaagcg accgcgccgg accgcccgcg
     4501 agtagccgac cctgctctcc cataccgtgc cgctggttac gttctcccgc ttcattatag
     4561 ttccccgacg aaaagaacta cccaattcta cctctctaca gtcacagcag ccctcggcgt
     4621 tcgagctcct ctctgaaacg tgttccttcc gctgaatctt ccttatagaa gacggcgggc
     4681 tctccgatgg gagccgggac cgtctcgttg atgccgcgct gcggcgaacc gtatctctct
     4741 cctgtccaga ggtggaccca tttgcccgcc gggaggtaga cctctacgct gtcttcgcca
     4801 gggtcgagca caggcgcaac catgagctcg gggccgacca tgaactggta ctccaggccg
     4861 gacacctcgg ggtcgttgga atagtggata aatgggtgcc tgaggacagg gaggcccgtc
     4921 tctgaggctg ctttcaccag gtccatcctg tagggcttcc agccggcgta gatcttcgcg
     4981 aagcgggtga agtgcgtgag ggtctctctg tccgagtaga tctggtggtt gacctcgggt
     5041 atgttgccct cgtgggtacg gaagacggtc gtaaaggcgc cgaattcgat ccagcgcaat
     5101 aggagttctt tggagcggtg gtacttgagc agtggatagt ccagggcggt gtagccgccg
     5161 atgtccgagt gttcgaggct atagcctgag agcccactgg agagcaggcc cgtgacggcg
     5221 ctcttgatgc cgtcgtgctc atcccagtcg acgagctggt ctccgaccca gaacagggtg
     5281 ctgtaacggg gactcttcgt gtaacctgaa cggttgaaga agacgatatc ttcgccccgg
     5341 gcggcctcgc tgatcgcctc gcggttcacc ctggcccact cctcggcgta gcggtcgtgg
     5401 taactcttcg ggtcggcgcc agagaatagc acggcgtcgt agggtagccc ttcgccaaag
     5461 tcggccatcc accctgaggc cccgttaccg atgagctccc ctttgattat gtccttgatc
     5521 caattccacg cctcagggtt cgtcagatcc acgagggccg cagagaagct ggagatcgtg
     5581 atcgtgtacg gctctccatc ctgcttcttt acaaggaagc cacttcttgc ggcttcctcg
     5641 aagagattgc agcggtgatc cttctcctgc gcggcgtcgt cacagaggaa tgggtttatg
     5701 taggtcatga gctttatatt cttctcttcc agaccctctc tcaatgaacc ccagttgggg
     5761 tagtgatccc tgtccagctc ccagttccac cagagctggg tgccgaagga ggtcttccgc
     5821 tgccccaccc agtcctgtag ccagagggcg gctatgggcg taccaagaga ttccagctcg
     5881 tctgagatcc ggcgcaccct gtccgtgccg ccctgcagtc ccacaaccgc gccgctgatg
     5941 atccacgctg gcagcgggcg catcctgcca gaatattcgg tgtactgttc gatcagctgt
     6001 gcgggagtgt ctccgcccag gatctgaccc ctcatcaggg aggagaaaac cccgatctgg
     6061 accctgtcgc cctccctgag gtcgaaggtc gagtactcgt agttctccag gaagagggaa
     6121 cgcgcctcac tcgtcatgta gtgcggcacg ctggcgtaac tggtgtaggg atcaccgccg
     6181 gcgccggctc tccagtcgac tgcccaggtt acgggttgtt cgcccctgcc tatgccctgc
     6241 tccctgatga ggatgggcac gatgcgtccc ttcatgtcga agtacgtgaa ctgggtcccg
     6301 aaaccgaaga agtgctcttc cggggaagaa gcgtag
//
LOCUS       DATMJA010000238         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_32574, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000238 DATMJA010000000
VERSION     DATMJA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32574"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1937
                     /gene="treY"
                     /locus_tag="VNT12_05670"
     CDS             <1..1937
                     /gene="treY"
                     /locus_tag="VNT12_05670"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HWI45639.1"
                     /translation="FFAVNDLAGIRVEDERVFEATHKLVLRLVEKGAVNGLRIDHPDG
                     LRDPGDYLRRLHNAAVEVSGGQIYTLVEKILAHHERLPEEWSVAGTTGYEFTNLVNGL
                     FVDPAGEGRMDEAYQSFIGRPLDFQSLLYECKHKVMRTALSSELNALSRRLRRISEYG
                     RSHDFSVETLREGLSEVVAHFPVYRTYIGAGTISETDRRHVDLAVHHAKERVEGGDPK
                     VFDFIRDIVLRQGPEEIEAGAFTAKFQQYTGPVMAKGMEDAALYVYNRLISLNEVGGE
                     PERFGVSVPEFHRLNAESIECWPHSMLSTSTHDTKRSEDVRARIDVLSEIPGEWRERV
                     VRWSELNAPHRRDAGGELAPSHNDEYLLYQTLLGAWALGEPDAEGLEVFRGRIKAYME
                     KAMREAQVRTSWTDVNEGYEEGVAGFVDVLLSATNPFLQEFLPFQRRMARIGALNSLS
                     QSLIKLTVPGVPDVYQGNEVWDFSLVDPDNRRPVDFDLRKRLLDDLKRLDLSNARALL
                     KDGSWQDGRPKLYLIWKALEMRRESSELFEDGSYVALQAVGERREHIVAFARRYGGEV
                     AITVAPRLYAKMMDAEGPLVAAPEAWGDTSILLPAELAEVSYRNVLTGQIVVAEEQMS
                     LGIGRLLRNFPVALLGSGPR"
     gene            2086..>2495
                     /gene="msrB"
                     /locus_tag="VNT12_05675"
     CDS             2086..>2495
                     /gene="msrB"
                     /locus_tag="VNT12_05675"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWI45640.1"
                     /translation="MTDTPRKIQKTEEEWQDQLTSEQYRITRMKGTERPFSGEYYDNK
                     VEGVYRCVACGTPLFASATKYDSGTGWPSFWEPLDEANVETEEDRSLFMRRTEVHCAV
                     CGAHQGHVFEDGPAPTGQRYCINSAALRFDPETKP"
BASE COUNT          504 a          734 c          810 g          447 t
ORIGIN      
        1 gtttcttcgc cgtcaatgac ctggccggaa tccgggtcga ggacgaacgg gtctttgagg
       61 cgacgcacaa gctcgtattg cggctggtgg agaagggcgc ggtgaacgga ctgcgcatag
      121 accaccctga tggactccga gatcccggcg actacctcag gcgcctgcat aacgcggctg
      181 tggaagtatc gggcgggcag atctacacgc tggtcgagaa gatcctggcg caccacgagc
      241 gtctgcccga ggagtggtct gtcgccggca cgacgggcta cgagttcacg aacctggtca
      301 acgggctatt tgtggacccg gccggggagg gcaggatgga tgaggcctat cagagcttca
      361 tcggacggcc cctcgacttc cagagtctgc tgtacgagtg caagcacaag gtcatgcgca
      421 ccgcgctttc gagcgagttg aacgcgctct cgcgccgtct gcggcgcatc tccgagtacg
      481 ggcgctctca cgactttagc gtggagaccc tgcgtgaggg gctctccgaa gtggtcgcgc
      541 acttccccgt ctaccgcacc tacataggtg cggggactat ctctgagaca gacaggcgcc
      601 acgtggatct ggcagtgcat cacgcgaagg agagggtcga gggcggcgac ccgaaggtct
      661 tcgatttcat ccgcgacatc gtccttcgcc agggacctga agagatcgag gcgggtgcct
      721 ttacggcaaa gtttcagcag tacacgggac cggtgatggc gaagggcatg gaggatgcgg
      781 ccctctacgt ctacaaccga ctgatctccc tgaacgaggt cggcggcgag ccggagcgct
      841 tcggcgtctc cgtccccgag tttcaccgtc tgaacgccga gagcatcgag tgctggccgc
      901 actcgatgct ctcgacctcc acgcacgaca ccaagcgcag cgaggacgtg cgcgccagga
      961 tcgacgtact ctcggagatc cccggtgagt ggcgcgagag ggtggtgcgc tggagtgagc
     1021 tcaatgctcc gcaccgccgc gacgctggtg gggaactcgc cccgagccat aatgacgagt
     1081 acctcctgta ccagacgctg ttgggcgcct gggcgctcgg agaaccggac gcagaggggc
     1141 tggaagtgtt tcgagggcgc ataaaggcgt acatggagaa ggcgatgcgg gaggcgcagg
     1201 tacgcacgtc ctggaccgac gtgaacgagg ggtatgagga aggggtcgcc ggtttcgtgg
     1261 acgtccttct ctctgcgacg aaccccttct tgcaggaatt tctgcccttc cagcgtcgca
     1321 tggcccgcat tggcgctttg aacagcctat cccagagcct gatcaagctg acagtacctg
     1381 gtgtccctga cgtgtatcaa ggtaacgagg tctgggactt cagcctcgtt gaccctgaca
     1441 accgccgccc ggtggatttc gatttgcgga agaggctcct cgatgacctg aagcggctcg
     1501 acctctccaa tgcacgcgcg ctcctcaagg atggttcgtg gcaggatggg cgtccgaaac
     1561 tttacctcat ctggaaggcg ctggagatgc gccgcgaaag ctccgaactc ttcgaagacg
     1621 gcagctacgt agcacttcag gccgtgggtg aaaggaggga acacatcgtc gctttcgccc
     1681 ggcggtatgg gggagaggtg gcgatcaccg ttgctccgcg cctctacgcg aagatgatgg
     1741 acgcagaagg accccttgta gcggctcccg aagcctgggg agatacgtcg atactccttc
     1801 ccgctgagct ggcagaggtc tcgtaccgca acgtgctcac aggccaaata gtggtcgccg
     1861 aggagcagat gtcgctgggc atcggtcgtc tgcttcgcaa cttccccgtg gccctgctgg
     1921 ggtccggacc tcgatgacgg gcggctcgac tttggagccg tctaccagaa cccgtgttac
     1981 ttccgaaacg ccgataggaa gtcgaaacct taagagagga ttaaacaggg gacgtgcgtt
     2041 gttcgcctcc tcaatagctg ggaacgggcg tggtagactt tccacatgac tgatacgcca
     2101 aggaagatac agaagacgga agaagagtgg caggaccagc tcacctctga gcagtaccgc
     2161 ataacgcgga tgaagggcac agagcggccc ttcagtggtg agtactacga caacaaagtg
     2221 gagggagtct accgctgtgt ggcctgcggg acgcccctat tcgcgtcggc caccaagtac
     2281 gattccggta cgggctggcc gagcttctgg gagccactcg acgaagcaaa cgtcgagacg
     2341 gaagaggatc ggagcctctt catgcgccga actgaggtac actgcgccgt ctgcggcgcc
     2401 caccagggac acgtcttcga ggacggaccg gcccctacgg gccagcgcta ctgcatcaac
     2461 tccgccgcac tccgcttcga tccagaaacc aaacc
//
LOCUS       DATMJA010000239         2837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_91706, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000239 DATMJA010000000
VERSION     DATMJA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91706"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1407
                     /gene="secY"
                     /locus_tag="VNT12_05680"
     CDS             127..1407
                     /gene="secY"
                     /locus_tag="VNT12_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWI45641.1"
                     /translation="MLGSLANAWKIPELRMRLLFTFAMLAAYRLGAYIPLPGVDPSVL
                     GGGLQTSNPITGLFGTFTGGAFNNFSVFSLGIMPYITAAIVMQLMTVAIPRLQELAKE
                     GETGQQKITQYTRYFTLALALVQSVAMVLFFRSAQGGGALAGTSPIEILVAIVTLTAG
                     VMLTMWLGELISQRGLGNGISLIITASILSRAPQAVQALRENGDVLTLVILVLLAVVI
                     VAAIVFVNEGQRRIPITYAKRQVGRRMSQGGTTYLPLKVNMAGVIPIIFASSLLLFPV
                     VLAQLAAGGDPDSILARLAAFFDPGNAPYLILYALLIIMFTYFYTAVQFNPVEHADNL
                     KKSGGYVPGYRPGQPTAIYLNNVLTRITLFGALFLAVVAVLPYLISGGLNLGNTIFLG
                     GTSMLIVVGVSLDTVRQLESQLMMRNYEGFLKKR"
     gene            1404..2036
                     /locus_tag="VNT12_05685"
     CDS             1404..2036
                     /locus_tag="VNT12_05685"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWI45642.1"
                     /translation="MKVILLGPQGAGKGTQAQRLADEVGATHISTGDIVRAEIKSGSE
                     LGKKVQDYNDRGELVPDEIIVEMAKPYLDDADSWILDGFPRNEAQAKALDQALEEIGE
                     DLDAVVALEAPDDALVERLSGRRQSQATGKIYHIEYDPPPEDDPGPFVQRDDDAEEAI
                     RRRLEIYHDQTEPLKDYYADRDLLVTVDAEQEIPKVTEDILGSVGREEDA"
     gene            2033..2797
                     /gene="map"
                     /locus_tag="VNT12_05690"
     CDS             2033..2797
                     /gene="map"
                     /locus_tag="VNT12_05690"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWI45643.1"
                     /translation="MIVRKSTTELEAMREGGRITAACLEMLEKNVQPGVTTRELDRLA
                     EEFIYEHGGKPEFKGYQGFPASICASPNAMIVHGIPGSYRLKNGDILSLDVGVRYEGF
                     VTDSATTVPVGHVPEDVVGLLGTTRECLAAATDQMRAGNRLGDVGHAIQALAESRGYG
                     VIRDLVSHGVGRQMHEDPQIPNYGSPGTGPRLLPGMTFAIEPMITLGDYDIRLSEWDG
                     WSIYTADGSLSAHFENTVAVTENGPLVLTQNGGSAG"
BASE COUNT          558 a          891 c          884 g          504 t
ORIGIN      
        1 catcaagatc cttggcgaag gggacgtagg ccgcgccgtt acggtaaagg cgcacgcttt
       61 ctccaggtcg gccagggaaa agatagaggc tggcggcgga agcgtcgagg tactggagaa
      121 gtagagatgt tgggatccct agccaacgcc tggaaaatcc cggaactgcg gatgaggctc
      181 ctgttcacgt tcgccatgct ggcggcgtac cgtctggggg cctacattcc cctgcctggg
      241 gtcgacccga gcgtcttggg tggcggtctc cagaccagca accccataac gggcctgttc
      301 gggacgttca cgggcggagc cttcaacaac ttctcggtgt tctcgctggg gatcatgccc
      361 tacataacag ccgcgatcgt catgcagcta atgacggtcg ccataccacg cctgcaggag
      421 cttgccaaag agggtgagac agggcagcag aagatcacac agtacacccg ctatttcacg
      481 ctcgcgctgg cgctcgtcca atcagtcgcg atggtcttgt tcttccgctc ggcgcagggc
      541 ggcggggcgc tcgccggtac tagccccatc gagatactcg tcgccatcgt taccctcacg
      601 gccggcgtga tgctcaccat gtggctcggc gagctcataa gccagcgggg tctcggtaac
      661 ggtatctcgc tcatcataac ggcctcgatc ctctccaggg cgccgcaggc cgtgcaggcg
      721 ctcagggaga acggtgatgt gcttacgttg gtcatcctcg tgttgctcgc cgtcgtgatc
      781 gtcgctgcca tcgtgttcgt caatgaaggc cagcgcagga tcccaataac ctacgccaaa
      841 cggcaggtcg ggcgcaggat gagccagggc ggcactacat acctgcccct caaggtcaat
      901 atggcagggg tcattcccat aatcttcgcc tcctcgctgt tgctgttccc cgtcgtgctg
      961 gcgcaacttg ctgcgggagg agacccggac tcgatcctcg cccgtctcgc ggccttcttc
     1021 gatcctggga acgcccctta cctgatcctc tacgcgctgc tcatcatcat gtttacctac
     1081 ttctacacgg cggtgcaatt caatcccgtc gagcacgccg ataacctgaa gaagagcggc
     1141 ggttacgtcc ctggctacag accgggccag ccgacggcca tctacctgaa caacgtgctg
     1201 accaggatca cgctcttcgg cgccctgttt ctggccgtgg tcgccgtgct gccttactta
     1261 atctcgggcg gcctgaacct cggcaacacc atctttctcg gcggcacctc gatgcttatc
     1321 gtggtcggcg tctcactgga cacggtgcgc cagctggaga gccagttgat gatgcgcaac
     1381 tacgaagggt tcctgaaaaa gcgatgaagg tcatcctgct tggaccccag ggagcgggca
     1441 agggaacgca ggcgcaacgg ctcgcagacg aggtgggcgc cacgcacatc tccaccgggg
     1501 acatagtgcg cgccgagatc aagtcgggct ccgagcttgg caagaaggtg caggactaca
     1561 acgaccgcgg cgagctggtg cccgatgaga tcatcgtcga gatggccaag ccttacctgg
     1621 acgacgccga ctcatggatc ctcgacggtt tcccccgcaa cgaggcccag gcgaaggccc
     1681 tggatcaggc cctggaagaa atcggcgagg atctcgacgc cgtagtcgcc ctcgaagcgc
     1741 ccgacgacgc gctcgtagag cggctctcgg gaaggcgcca gagtcaggct acgggcaaga
     1801 tctaccacat cgagtatgat ccgccccccg aggatgaccc aggcccgttc gtccagcgcg
     1861 atgacgacgc cgaggaagcc atccgtcgca ggctggagat ctaccacgac cagaccgagc
     1921 ctctgaaaga ctactacgcc gaccgcgacc tcctcgttac ggtcgacgcc gagcaggaga
     1981 tcccgaaggt cacagaagac attctcgggt ccgtaggcag agaggaagac gcttgatcgt
     2041 ccgcaagagc acgacggagc tcgaggcgat gcgtgagggt ggaaggataa ccgcggcttg
     2101 cctggaaatg ctcgaaaaaa acgtgcagcc aggcgtgacg actcgggagc tcgaccgtct
     2161 cgccgaggag ttcatctacg agcacggagg caagcccgag ttcaaggggt atcaggggtt
     2221 cccggcttcg atctgcgcct cgcctaacgc catgatcgtg cacggcatcc ctggctcata
     2281 ccgcctgaag aacggcgaca tactctccct ggacgtcggc gtccgctatg agggcttcgt
     2341 aacagactcg gcgaccacgg tccctgtcgg gcacgtgccc gaggatgtcg tcgggttgct
     2401 cggaaccacg agggagtgtc tggccgcggc gacggatcag atgcgggctg gtaacaggct
     2461 tggcgacgtg ggccacgcca tacaggctct cgccgagtcg cgaggttacg gggtcataag
     2521 agatctggtc tcccacgggg tcggccgcca gatgcatgag gacccccaga taccaaacta
     2581 tggcagcccg ggtacgggac cgcgtctgct ccccgggatg accttcgcca tagagcccat
     2641 gatcacgctc ggcgactacg acatccgcct tagcgagtgg gacgggtggt ccatctacac
     2701 ggccgacggc tccctctccg cccacttcga gaacaccgtc gccgttaccg agaacggccc
     2761 cctggtgttg acgcagaacg ggggctccgc cggatagaga aaggcgttcg tcggtgggag
     2821 gggcacctcc gacgtgg
//
LOCUS       DATMJA010000240         4420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_328926, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000240 DATMJA010000000
VERSION     DATMJA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328926"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VNT12_05695"
     CDS             complement(<1..322)
                     /locus_tag="VNT12_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWI45644.1"
                     /translation="MTEQKYFRKGFKMRENVQHLLDRDYRSEVVDRVRENGNVFEADG
                     LTIRLADEFGFCYGVDRAVDYAFQTKEKFPDRRIFITGDMIHNASMNDRLREMGIEFL
                     STGFE"
     gene            436..846
                     /locus_tag="VNT12_05700"
     CDS             436..846
                     /locus_tag="VNT12_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI45645.1"
                     /translation="MGVKSIAVDRRATIMVADDDVEALGRSASALEDAGFRVVSATDG
                     RTALQEALTRRVDLIIMDVSIPQLSGVETCHCLKAMPKTQKIPVVLTAAKKDPAAKAL
                     AQRTQGSVRVLRKPFTDEDLLSVVRQVARPKSLI"
     gene            930..2453
                     /locus_tag="VNT12_05705"
     CDS             930..2453
                     /locus_tag="VNT12_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HWI45646.1"
                     /translation="MGLSKRRKALITLATLIGTFLAALDATVVWTAMPTVIGELGGLE
                     LYPRAFASYLLAATLTGPVFGKLSDTYGRKPVYLAGIFLFLLGSVLAGTSQSMAALIF
                     FRTIQGLGAGAVQPVAITIVGDIFELEARARIQGLFGAVWGISAVVGPAAGGFITDLI
                     SWRWVFYVNVPFGIIAAVLLAATLTESFERRERSVDYLGTALLTGGLVAVLLALLGGG
                     GAGERSVTTLGLFFGGLAVLALFVLVERRAEDPVVPLDLFSERIIAVSALGNVAMGGV
                     LLGVSVYVPLFVQGALGGTAITAGAAVASLSIGWPVGSFVGGRLLLLTGYRATLLLGS
                     ALIALGSALCVPMDSGTSLSYVVLAVVVIGLGLGFSSTSYLVSVQNAVPWRRRGVATS
                     SVVFFRTVGGSIGVAVMGAFLNASLGERYRDAVDRAAGGDPGLGRLLSDPNALLQPAV
                     RSRIPEGAYSEVAGALAASLSPAFWVLLFFGLSALAVATLFPHGTAKDLVSREEDYG"
     gene            2446..3099
                     /locus_tag="VNT12_05710"
     CDS             2446..3099
                     /locus_tag="VNT12_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45647.1"
                     /translation="MGETGRELTLEDLPSPPLFKLIDPEGRDVFQETEVGGEGAKVCV
                     LFSDRELAGEFSAGAAAHGMESLSGLDPRELSDWSSVEVFALSGADYVLVVSERGAGL
                     FHAGDVAQKAEEMAGEIPFPLYMISDERGEAPLIKVEVDDGEVLVAALFSSLENARDF
                     RERASHLNLPDSLGTIDDRDGLRRHALVAREAGATYAVIDPASGLTEAIPIEELSVP"
     gene            3096..3683
                     /locus_tag="VNT12_05715"
     CDS             3096..3683
                     /locus_tag="VNT12_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWI45648.1"
                     /translation="MKPTLCTDVDSSIWDTGAWVCSAVLDITGDTLDMDTITTWTHVL
                     DTYGEKTTTAIFDRVFDPERVRDREPYPGAPEVLRALQERHGIEVHFVTHNDPELMIP
                     HLEPWLRTHFGPNVGLTVTTEDKLDILEQLGALGLIDDRPDTIGRVADAGLWAAAMIQ
                     PWNRDLLTRRRDIHGFSHWQEVPALLPAEYRTSIV"
     gene            3838..4071
                     /locus_tag="VNT12_05720"
     CDS             3838..4071
                     /locus_tag="VNT12_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45649.1"
                     /translation="MEDAESKEGMWKDAPRIEGFLGRGRPVTVSARTGVDLEGYVCDQ
                     NESGLLLDARDSSGDPTGYEFLPWSSIERVSAG"
     gene            4195..>4420
                     /locus_tag="VNT12_05725"
     CDS             4195..>4420
                     /locus_tag="VNT12_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45650.1"
                     /translation="MGGGLILRRARREDAEAMAAFNAKVHHSSDGAFEQREPHRGVAA
                     FTRDLMSGDHPACDASDFTVIEDTMTGSIVS"
BASE COUNT          812 a         1421 c         1364 g          823 t
ORIGIN      
        1 cttcgaagcc ggtggagagg aactctatcc ccatctcgcg caggcggtcg ttcatagatg
       61 cgttgtggat catgtcccct gtgataaaga tgcgcctgtc agggaacttc tccttggtct
      121 ggaaggcgta gtccacagcc ctgtccaccc cgtagcagaa cccgaactca tcggcgaggc
      181 gaatcgtgag gccgtcggcc tcgaagacgt tgccgttctc acgaaccctg tcaacgactt
      241 cagagcgata gtcccggtcc agaagatgct ggacgttctc ccgcatcttg aagcctttac
      301 ggaagtattt ctgctccgtc acgctcacct ctcgtacccg acgttcgttt ctcgtgctcg
      361 aggctgagtg tagcgcatct accccgccgc gtcggagggg gttgtggcac atatcacagt
      421 cgctgcaaat gtaaaatggg tgtaaaatct atcgccgtgg accgcagagc aaccatcatg
      481 gtcgcagatg acgacgtaga agccctggga cgctccgcca gcgccctgga ggatgcgggc
      541 ttccgcgttg tctcggcgac ggacggccgc acggccctcc aggaggctct cacccgcagg
      601 gtagacctca taatcatgga cgtttcgatc ccccagctga gcggtgtcga gacgtgtcac
      661 tgcctcaagg cgatgcccaa gacccagaag atccccgtag tactcaccgc ggccaagaaa
      721 gatccggccg ctaaagcgct cgcccaacgc acgcaaggct cggtgcgggt actgagaaag
      781 ccctttacag acgaagacct cctctccgtc gtcagacagg tcgctcgccc caagtccctg
      841 atctagggta tctggttcac ctcacgcgtc tcagagcgac gcggtggtaa gatcgctccc
      901 cgtaaaagct gccacaggag cactgtttct tgggactgag caagcgccgc aaggcgctca
      961 taacgctcgc cacgctcatc gggacctttc tggccgcact cgacgccacg gtcgtatgga
     1021 cggcgatgcc gaccgtgatc ggggagctcg gcggcctgga gctctatccg cgagccttcg
     1081 cctcttacct actcgcggcg accttgacag gccccgtctt cggcaaactc tccgatactt
     1141 acggcagaaa acccgtctac ctcgccggga tcttcctctt cctgctcggc agcgtgctcg
     1201 cagggacctc acagagcatg gcggccctca tcttcttcag aacgatccag ggtctcggag
     1261 ccggggccgt gcaacccgtc gcaataacca tagtagggga catattcgag ctcgaagcgc
     1321 gggcgcgcat ccagggtctc ttcggggcgg tgtgggggat ctctgccgtg gtcggccccg
     1381 cagccggcgg cttcataacg gatctgatct cctggcgctg ggtcttctac gtgaacgtgc
     1441 ccttcggcat catcgccgcc gtcctcctcg cggcgaccct gaccgagtcc ttcgagcgtc
     1501 gcgagcgcag cgtggactac ctcggcacag ccctccttac aggcggcctc gtcgccgtgt
     1561 tgctcgcgct gctcggaggt ggaggcgccg gggagcgctc ggtgacaacc ctcgggctgt
     1621 tcttcggcgg gttggccgta ctcgcgctct tcgtgctcgt cgagcggcgg gctgaagacc
     1681 ccgtggttcc gctcgacctg ttctcggagc ggatcatcgc ggtctccgcg ctagggaacg
     1741 tggcgatggg cggcgtcttg ctcggcgtct cggtctacgt gccgctcttc gtgcaaggcg
     1801 ccctcggagg cacggcgatc acggctggcg ccgccgttgc ttcactctcc atcggctggc
     1861 ccgtaggatc tttcgtcggc gggaggctgc tcctgctcac ggggtaccgc gcgacgctcc
     1921 tactcggctc ggccttgatc gcccttggct cggccctctg cgtgcctatg gatagcggaa
     1981 cctcgctttc ctacgtcgtg ttggccgtcg tggtgatcgg gctggggctt ggcttctcga
     2041 gtaccagcta cctcgtcagc gtgcagaacg ccgttccctg gcggcgtcgc ggtgtggcca
     2101 cctcctcggt agttttcttc cgtaccgtcg gaggatcgat cggggtcgcc gtgatgggcg
     2161 ctttcctgaa cgcctccctc ggagagcgct acagggacgc ggtcgacagg gcggcagggg
     2221 gcgatccggg cctcgggcgc ctgctctccg accccaacgc tcttctgcaa ccggccgttc
     2281 gcagccgcat ccccgagggg gcgtactcgg aggtggccgg cgccctcgcc gcctcgctca
     2341 gcccggcgtt ctgggttctg ctcttcttcg gtctctcggc cctcgccgtg gctacgctct
     2401 tcccacacgg aacggccaaa gacctcgtaa gcagggagga ggattatggg tgagacggga
     2461 cgcgaactca ccctggaaga cctccccagc ccgcccctct tcaagctgat cgatcccgaa
     2521 ggaagggacg tctttcagga gaccgaggtc ggcggcgaag gggccaaggt ctgcgtcctg
     2581 ttctcggaca gggagctcgc aggagagttc tctgcaggtg cggccgcgca cggcatggaa
     2641 agcctctctg gcctggatcc gagggagctc tccgactgga gctcagtaga agtcttcgcg
     2701 ctctctgggg ccgactacgt gctcgtcgtc tccgaaagag gcgccggact gtttcacgcc
     2761 ggtgacgtgg cccagaaggc ggaggagatg gccggtgaga tccccttccc gctgtacatg
     2821 atctccgacg agaggggaga ggcgcccttg atcaaggtag aggtcgacga cggagaggtc
     2881 ttagtagcag cgctcttcag ctcactcgag aacgcccgcg acttccgcga acgtgcctcc
     2941 cacctcaacc tgccggacag cctcgggacc attgatgaca gagacggcct cagacgccac
     3001 gcgctcgtag ctcgcgaagc gggggcaacc tacgctgtca tagaccccgc atcaggcctc
     3061 accgaagcga tcccgatcga agagttgtca gtaccttgaa accgaccctc tgcacggatg
     3121 ttgactcgag catatgggac acgggcgcct gggtctgttc ggcagtcctc gacatcacag
     3181 gcgatacgct ggatatggac acaatcacca cctggaccca cgtactcgac acctacggcg
     3241 aaaagacaac aaccgccatc ttcgaccgcg tcttcgaccc tgagcgcgtc cgggatcggg
     3301 aaccctaccc tggcgcccca gaggtgctac gcgcattgca agaaaggcac ggcatcgagg
     3361 ttcacttcgt aacccacaac gatcccgagc tcatgatccc tcatctcgaa ccctggctca
     3421 gaacccattt cggcccaaat gtgggactta cggttacgac ggaggacaag ctcgatatcc
     3481 tggaacaact gggtgcctta ggcctcatag acgacaggcc tgacaccata gggcgggtcg
     3541 ccgacgcagg tctgtgggcg gcggccatga tacagccctg gaaccgcgac ctcctgacca
     3601 ggaggagaga catacacggc ttctcccact ggcaagaagt cccggctctc ctccccgcag
     3661 agtacaggac aagcatcgtt tagaggccat ctgcccccgc atcgacccac cacggatgaa
     3721 agctgaccag cggaggccgt aaggccgaag tgtgctgaaa tgctgagacg cgcccgacag
     3781 ggcgcgtgcc gtactgtaac cttccgtgtg tcagatacgg gacaacaagg agacacgttg
     3841 gaagatgctg agagcaagga gggcatgtgg aaagacgcgc cgcggatcga gggtttcttg
     3901 ggccgtggga gaccggtcac ggtctcggct agaaccggag tcgacctgga gggctacgtc
     3961 tgcgaccaga atgagagtgg cctgctcctc gacgccagag actccagcgg ggacccgacc
     4021 ggctatgagt tccttccctg gtcaagcatc gagcgggtga gcgccggcta gagtcccgat
     4081 ctctcgaaac gaaaggatag gagataatgc aaccctccgg cgctcctcag aacgagagaa
     4141 aagcgatggc caacggacaa gatcctaagg ccctttctgg ggtgctaaag gatcttgggg
     4201 ggtggattga tcctgcgccg ggcaaggcgc gaggacgccg aggcgatggc tgcgttcaac
     4261 gcgaaggtgc atcactcttc cgacggggcg ttcgagcagc gagagcccca caggggggta
     4321 gccgccttca cgcgcgactt gatgtctggg gatcatcccg cctgcgacgc ctcggacttc
     4381 accgtaatcg aggacaccat gaccggctcc atagtctcct
//
LOCUS       DATMJA010000241         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_328941, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000241 DATMJA010000000
VERSION     DATMJA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328941"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VNT12_05730"
     CDS             complement(<1..241)
                     /locus_tag="VNT12_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HWI45651.1"
                     /translation="MAAWLERLGLGGREMTASGHSKNFVLRVVQPALIGLMDGSVSTL
                     APLIATAFATRDPGVTFLVGLAAAVGAGISMGFSER"
     gene            complement(456..3203)
                     /locus_tag="VNT12_05735"
     CDS             complement(456..3203)
                     /locus_tag="VNT12_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HWI45652.1"
                     /translation="MKRSKSADPAHDILGYERQQLVAIFRPETVAVIGASERPGSVGR
                     TIMWNLVSSPFGGTVFPVNSHRPSVLGIKAYPSVSEVPARVDLALIVAPAPAVPGIIS
                     ECVEAGVEGAVIISAGFRETGSEGAELERQVLEEARRGRMRIVGPNCLGVMKPPTGLN
                     ATFAGAIARPGNVALLSQSGALLTAILDQSFMENIGFSALVSAGSMMDVGWGDLIYYL
                     GDDPYTKSIVIYMESVGDARSFLSAAREVALTKPIIVIKAGRTEAAGKAAASHTGSLT
                     GSDEVLDAAFSRSGVLRVDEISELFGMAEVLGKQPRPKGPRLTILTNAGGPAVLATDA
                     LIGNGGELAEISEETMGALNEFLPAPWSHGNPLDVLGDADPELYAKTLETAAGDPQSD
                     GLLVILTPQDMTDPTATAERLAPYANSTSKPVLASWMGGPAVAAGESILNGAGIPTFD
                     YPDTAARAFTNMWKYTYNLRSIYETPEPAEEESAHRGRVEEIIDHVRDSGRTILTEFE
                     AKQVLAAYGIPTVQTEVARSPGEAVELAGSLGYPVVLKLDSETITHKTDVGGVRLNLQ
                     SADEVRLAYEEMESSIKENHGPEDFGGAAVQKMVSLDGYELIVGSSLDPQFGPVLLFG
                     SGGSLVEVYRDRALALPPLTTTLARRMMERTRIFEALGGIRGRAPIDVGSLERLLVRT
                     SQLVVEQPWIKELDINPLLASPERLIALDARIVLHDPEMKEVDLPRTAIRPYPKQYVS
                     QEVLRDGTPVTIRPIRPEDEPLMVRFHETLSEQSVYMRYFHMMNLDQRTAHERLTRIC
                     FIDYDREMALVAERTDPETGEREIMGVARLSRRGAVPEEAEFSILVSDRFQRRGIGTL
                     LLTRILEVARAEGLSRITAEILLDNRPMQRILKKLGFHLRRDTEEMVVKADLDLRQHV
                     S"
     gene            3552..3704
                     /locus_tag="VNT12_05740"
     CDS             3552..3704
                     /locus_tag="VNT12_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45653.1"
                     /translation="MSVMDNRGLVLDGNTGGTPTDNSGEPGAVQRHLLVAAALMTSGA
                     TLRRGR"
     gene            3717..>4009
                     /locus_tag="VNT12_05745"
     CDS             3717..>4009
                     /locus_tag="VNT12_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45654.1"
                     /translation="MRILDLTHRFEGDPLLKVGMGAMILALGFTIVVAVAAFLDRPIE
                     SAAAKPEVKSATEPLVRSDPDVDPWVEKDISPPRSQATTIPARPSEPDKPPEP"
BASE COUNT          748 a         1263 c         1229 g          769 t
ORIGIN      
        1 atctctccga gaagcccatg ctgatccccg ctcccaccgc agccgcaagc ccgaccagaa
       61 acgtgacgcc agggtccctc gtcgcaaacg ccgtcgcaat aagcggcgcc agcgtcgaaa
      121 ccgacccatc catcaaacct atcagcgccg gctgaacaac ccgcaatacg aagttcttgc
      181 tgtgtcccga agccgtcatc tcccggcccc caagtcccaa tcgctctagc caggccgcca
      241 aatactctcc tcccgaatac acgacgtata acccacccca aaattctaac cacactcagc
      301 tataaatcaa gaacgattat tgttactggt taatgggaat cattagcgat agggaagatg
      361 tggtcgagta agcccgcctc atccatcgcg cattcggcta cgaggcaaaa caagaagctg
      421 aagtgctgac aggcgcccga cagggcgcgt gctgactagc tgacatgctg acggaggtcg
      481 agatccgcct tcacgaccat ctcttcggtg tcgcggcgga ggtggaagcc gagcttcttc
      541 aagatgcgtt gcatggggcg gttatcgagg agtatctctg cggtgatacg gctcagaccc
      601 tcggcgcggg cgacctcgag gatgcgagtc aggagcaggg tgccgatgcc ccggcgctgg
      661 aagcggtcgc tgacgaggat cgagaactct gcctcttcgg ggacggcgcc gcggcggcta
      721 aggcgggcta cgcccatgat ctcacgttcc ccggtctctg gatcggttcg ctcggcgacc
      781 agcgccatct cacggtcgta gtctataaag cagatgcgcg tcaggcgctc gtgggcggtg
      841 cgctggtcga ggttcatcat gtggaagtag cgcatgtaga cgctctgttc cgagagggtc
      901 tcgtggaagc gcaccatcaa gggctcatct tcgggacgga tcgggcgtat cgtcacaggc
      961 gtgccgtcgc gcaacacttc ttgggagaca tactgtttcg ggtaaggacg gatcgcggtt
     1021 cgtgggagat ctacttcctt catctcaggg tcgtgcagaa cgatacgggc gtcgagagct
     1081 atcaggcgct ccggcgaggc caggagcggg ttgatgtcga gctccttgat ccagggctgc
     1141 tcgacgacga gctggctcgt gcgaacgagc agcctctcca gagagccgac gtcgatcggc
     1201 gccctgcccc tgatgccacc gagggcctcg aagattcggg tcctttccat catccgcctg
     1261 gcgagcgtgg ttgtcagcgg tgggagggcg agggcccggt ccctgtacac ctcgaccagc
     1321 gagccgccgg acccgaagag caggacagga ccgaactgcg ggtcaaggct gctgccgacg
     1381 atcagctcat agccgtcgag actgaccatc ttctggacgg cggcgccgcc gaagtcctcg
     1441 ggcccgtggt tctcctttat agaggactcc atctcctcgt aggccagcct gacctcgtcg
     1501 gcgctctgca ggttcaggcg cacgccgccc acgtccgtct tgtgggttat cgtctcggag
     1561 tcgagcttga ggaccacggg gtagccgagg ctacctgcca gttccacggc ctcaccaggg
     1621 ctgcgcgcca cctccgtctg gacggtcggg ataccgtagg cagcgaggac ttgcttcgcc
     1681 tcgaactctg taaggatggt acggccagag tcgcggacgt gatctatgat ctcctctacc
     1741 cgcccgcggt gcgcgctttc ttcttcggca ggctcagggg tttcatagat gctgcgtagg
     1801 ttgtacgtgt acttccacat gttcgtgaaa gcccgggctg ccgtatcagg gtagtcgaag
     1861 gtggggatgc cagcgccgtt caggatcgac tcccctgccg cgacggccgg tccacccatc
     1921 cagctcgcga gcaccggctt gcttgtgctg ttcgcgtagg gcgcgagccg ttccgccgtt
     1981 gctgtcgggt ctgtcatgtc ctgcggcgtg aggatgacca gcagtccgtc gctctggggg
     2041 tcgcctgccg cggtctctag cgtcttcgcg tagagttcag ggtcggcgtc gcctagcacg
     2101 tctagagggt tgccgtggct ccatggagct gggaggaact cgtttagagc tcccattgtc
     2161 tcctcggata tctccgcgag ttcgccgccg ttgccgatca gggcgtccgt cgcgagcacg
     2221 gcgggaccgc cggcattcgt caggatcgtc aggcgcggtc ccttggggcg cggttgtttg
     2281 ccaagcacct cggccatccc gaacagctcg gagatctcgt ccacgcgcag tacgccgcta
     2341 cgcgaaaagg cggcgtcgag cacctcatcc gagccggtca aagagcctgt gtgagaggca
     2401 gcagccttac ctgcggcctc ggtgcgtccg gctttgatca ctattatcgg tttggtcagg
     2461 gcgacctcgc gggccgccga gaggaacgaa cgggcgtcgc cgaccgactc catatagatg
     2521 acgatgctct tcgtataagg gtcgtcgccg aggtagtaga tcaggtctcc ccaacctacg
     2581 tccatcatgg aacctgcgct gacgagcgcc gagaatccga tgttctccat aaagctctgg
     2641 tccaggatgg ccgtgagcaa agccccgctc tggcttagaa gggccacgtt tccgggacgt
     2701 gcaatagcgc cggcgaaggt ggcgttgagt cccgttggtg gcttcatgac gccgaggcag
     2761 ttgggaccga cgatcctcat cctgccccgc ctagcctcct cgagtacctg gcgctcgagc
     2821 tctgccccct cggaccccgt ctccctgaaa ccagctgaga ttatcacggc gccctcgaca
     2881 ccagcctcta cacactcgct gataatccca ggcaccgcag gcgcaggggc gacgatgagc
     2941 gcaaggtcta ccctagccgg cacttcggag acggaagggt aagcctttat gccgagtacg
     3001 ctcggcctgt gcgagttcac ggggaagacc gttccaccga acgggctgct cacgaggttc
     3061 cacatgatcg tacgccccac gctaccagga cgctcggacg ccccgatcac agccaccgtc
     3121 tcgggacgga agatggcgac caactgctgc cgttcgtaac ccagaatgtc gtgggccggg
     3181 tccgcgctct tcgaccgctt catagtcgct cctttgccgc tcctggctgt cagcacgccg
     3241 ccctacgagc ggctcgtcag catttcagct tctccatatt ttaggtgccc cgaacggagg
     3301 aacctaacct cgtttcgtga ggtgggtcaa ctatgtacct cttgcactta tacctgtaac
     3361 attgggggta attacgtggc gtaaatttgc atacgagcac tgtcatccct agctctgtct
     3421 cggagattgg cgctcgaaga tgtagaacag gatacccgag cagtatatga tgcagtgtgc
     3481 atcgcaacgc tctgagggtt cccgagtaca gcctcgccgg ttgatcttag agcacagcgg
     3541 aaggggatgg ggtgagtgtc atggataaca ggggactcgt actcgacggc aacaccggcg
     3601 gcacgccgac agacaacagt ggggagcctg gcgcggtcca acgtcacctc ttggtagcgg
     3661 ccgcactcat gacatcgggg gcgactcttc ggcgcggcag gtagcgcgac tccgccgtgc
     3721 ggatcctgga tctaacccac aggttcgagg gagacccact cctcaaggtc gggatgggtg
     3781 ccatgatcct cgcgctcggg ttcaccatcg tggtggccgt cgccgccttc ctcgaccggc
     3841 cgatagagtc tgcagccgcg aaaccggagg tgaagtccgc caccgagcca ctcgtccgct
     3901 cagaccccga cgtcgacccg tgggtagaga aggacatatc tccgcccagg tcccaggcaa
     3961 caaccatacc agcccggcca tcggagccag ataaaccgcc agagcccag
//
LOCUS       DATMJA010000242         2888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_388394, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000242 DATMJA010000000
VERSION     DATMJA010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388394"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VNT12_05750"
     CDS             complement(<1..737)
                     /locus_tag="VNT12_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWI45655.1"
                     /translation="MAGGVDVRGRVEDLREQVRYHNRRYHVDDAPEISDAEYDALYRE
                     LEALEDEHPELITPDSPTRRVGGEPIEGFEEVRHSVPMLSLANARKIEDLREWDARVR
                     RLLGEDETPRYVTELKIDGLAVSLRYENERLVRGATRGNGVIGEDVTAQLRTIRSIPE
                     RLDDGPPEVLEPRGEAYIPIADFEKLNRELEGEGNKPYANPRNLAAGSIRQLDPKITA
                     RRPLTILLYGVGEGGEIFESHSEMLAAL"
     gene            977..1438
                     /locus_tag="VNT12_05755"
     CDS             977..1438
                     /locus_tag="VNT12_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45656.1"
                     /translation="MRRTATILAVVALMVVSSSGVALAAYEDLITGTDGVDIISGTGK
                     SEHISGLGGDDRLNGGDGADLIEGDLGSDELGDGGGRDIVNGNDGADNLIGQGGDTSA
                     DRFYADSGSDTVQSRDVPAVKDTVECGAGTDTVYADKADVVADDCEQVRAW"
     gene            complement(1507..1812)
                     /locus_tag="VNT12_05760"
     CDS             complement(1507..1812)
                     /locus_tag="VNT12_05760"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWI45657.1"
                     /translation="MERANHRPDNPYTQLEGYRVNDASGEEVGEIENTVYDAPSDVLK
                     YVVVRGHPIPAERIEVDAADQRVSVPYDARTIESAPQMEEISGPFDEAIHEHYESRA"
     gene            1864..2853
                     /locus_tag="VNT12_05765"
     CDS             1864..2853
                     /locus_tag="VNT12_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45658.1"
                     /translation="MDSRTDQGWNARSLKAFSFVLLALMVASLLFVHTASYEPAVSYI
                     LLYLVSSVGIITAAVVVPLLPEEQFTAGFCGSIYALYTAVVAMMVFFSGGTSSELYVV
                     FFPLILASALHGSWRVVLAVLVAVLFCYALAMLPGLLERGTGQEIASLVFYRLTAVGL
                     TGLFLMYVARSVLPAGLAEDYAKDEDGSILLERVEEELAARRGAPVAVVLVDPGVEME
                     AVDLLLERVWTRIGEPVLLGEGNLFGVVVSGVGDGGVESAARRALAAESSLGAQDTRA
                     GAAIYPRDARSAEDLLIAAGRALEAAFEVESSSAIVLAGSGAPRGAGRYRAAR"
BASE COUNT          473 a          887 c          937 g          591 t
ORIGIN      
        1 agcgcggcga gcatctccga gtggctctcg aagatctcac ccccctcccc gacgccgtag
       61 agcagtatgg taaggggtct tcgggccgtg atcttcgggt cgagctgcct gatggatccg
      121 gccgccaggt tgcgcgggtt ggcgtatggt ttgttgccct caccttcaag ctccctgttc
      181 agcttctcga agtcagcgat ggggatgtac gcctcgccgc gcggctcgag tacttcgggc
      241 ggaccgtcat cgagacgctc gggaatcgaa cgtatcgtcc ttagctgcgc cgtaacgtcc
      301 tcgccgatca cgccgttgcc gcgtgtggcg cccctgacca gccgctcgtt ctcatagcgt
      361 agcgagaccg ccaggccgtc tatcttcaac tccgtcacgt agcgcggggt ctcatcctcg
      421 ccgagcaggc gccgcacgcg ggcgtcccac tccctcaggt cttctatctt gcgggcgttc
      481 gcgagggaga gcatggggac agagtgcctg acctcctcga atccctcgat gggctcgccg
      541 ccgacgcgcc gggtcggtga gtcaggggtg atgagctcgg gatgctcgtc ctccagggct
      601 tccagctccc tgtagagagc gtcgtactcg gcgtcggaga tctccggcgc gtcatctacg
      661 tggtagcggc ggttgtgata acggacctgc tcgcgcagat cctctaccct accccgaacg
      721 tccacacctc cggccacgca gggattatac gcacctaagc acccgctccc aagagaagct
      781 tgatcgctac tgtgccggac agggcctgaa tgcgcgcctg catgaggcgt tccgcaacct
      841 tttgctccac aggccgttac cgaaacgtta ataggaccgt ggccgaacgt ttacttatct
      901 gtaacgtggg tatcgtaaag gaggtaggta acggcccgtt gagaccgacg ggaccgacta
      961 ggagggggat acgtagatga ggcggacggc gacgatactg gcggttgtgg ccctcatggt
     1021 ggtgtcctct tccggcgtgg ccttggcggc ttacgaggac ttgatcacag gcaccgacgg
     1081 cgtcgacatc atctcgggca cagggaagtc ggagcatatc tccggcctcg gtggcgacga
     1141 ccggctcaac ggcggcgacg gcgccgacct catcgagggc gacctgggtt ctgacgagct
     1201 cggcgacggg ggtggacggg acatcgtcaa cggcaacgac ggcgcggaca acctgatcgg
     1261 gcagggtggc gatacctcgg ctgaccgctt ctacgctgac agcggcagcg acaccgtcca
     1321 gtcgagggat gtccctgccg tcaaggacac tgtggaatgc ggcgccggca cagacaccgt
     1381 ctacgccgat aaggccgacg tcgtcgcaga cgactgcgag caggtaaggg cctggtaaac
     1441 ctactgtccc ggtctgataa cgaggctagg aagggccagc ccttcctagc ctcgttatga
     1501 aatgcttcaa gcgcggctct cgtagtgctc gtggatggct tcgtcgaaag gtcccgaaat
     1561 ttcctccatc tgtggtgctg actctatggt tctcgcgtcg taggggacag agacgcgttg
     1621 gtctgcggca tctacctcta tcctctcggc tgggatgggg tgccccctga cgacgacgta
     1681 cttcagcacg tcgctcgggg cgtcgtaaac ggtgttttcg atctccccga cctcctctcc
     1741 cgaagcgtcg ttgaccctat agccctccaa ctgcgtgtag ggattatcgg ggcgatggtt
     1801 tgctctctcc atacttcatc ctccggtggc aggcatacga gtatatgcgt atgatacttc
     1861 ggcgtggaca gcagaacaga tcaaggctgg aacgcccgga gcctgaaggc tttctcattc
     1921 gtgctcctgg ccctcatggt ggcttcgctg ctgttcgtgc acaccgcttc atacgaacct
     1981 gcggtctcct acatcctgct ctatctcgtc tcctccgtag gcatcatcac cgcggcggtc
     2041 gtggttccgt tgctgccaga ggagcaattc accgccgggt tctgcggctc gatttacgcc
     2101 ctgtatactg ctgtcgtcgc catgatggtc ttcttctcgg gtgggacatc gagcgaacta
     2161 tacgtcgtat tcttcccgct gattctggcc tcggcgctgc acgggtcgtg gcgggtagtg
     2221 cttgcggtcc tcgtcgccgt ccttttctgc tacgccctgg cgatgctgcc aggccttctc
     2281 gagagaggga caggccagga gattgcctcg ctcgtcttct acaggctcac cgctgtggga
     2341 ctgaccggcc tgttcttgat gtacgtggcg aggagcgtgc tccctgccgg tcttgctgag
     2401 gactacgcga aggatgagga cggttccata ctgctcgagc gtgtcgagga ggagttagcg
     2461 gcgcgcaggg gggcgcccgt cgctgtggta ctcgtcgacc cgggagtgga gatggaggct
     2521 gtggatctcc tcctggagag ggtgtggacg cgcatcggag aacccgtgtt gctcggcgag
     2581 ggcaacctct tcggggtggt cgtaagcggg gtaggcgacg gaggggtaga gagtgcggcg
     2641 cggcgcgccc tggccgccga gagctctctc ggcgcgcaag acaccagggc aggtgcggcg
     2701 atctacccgc gcgacgcccg ttccgcagaa gacctcctga tcgccgcagg gcgggcgctc
     2761 gaagccgctt tcgaagtcga gtcatccagc gccattgtcc tggccggcag tggcgcgccg
     2821 cggggagccg gccgctacag ggccgcaaga tagctgtcag cacgccgcca ggctttgcca
     2881 agcggctt
//
LOCUS       DATMJA010000243         2749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_329184, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000243 DATMJA010000000
VERSION     DATMJA010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329184"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VNT12_05770"
     CDS             <1..613
                     /locus_tag="VNT12_05770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HWI45659.1"
                     /translation="LHHLWNRKVVRATGLGTGGRALDLACGTGTLTCDLAEKVGAEGY
                     VLGVDFSREMLHAARSAPNVEYRLGDATDLQSVPSGAFDAATIAYGARNIPDLDALFS
                     EMARTLRPGGVAVCLEIARPTGRISSAFYGVWFDKIVPRLGGWISGDPSAYAYLPESV
                     KEFVAPGELADIIGRNGLQNVTWERVAGGIITLHRGIRPNDAG"
     gene            528..1577
                     /locus_tag="VNT12_05775"
     CDS             528..1577
                     /locus_tag="VNT12_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWI45660.1"
                     /translation="MDYKTLHGSASQAASSRSIVGSGQTTQASRLHSALPEGAFEVID
                     EVEGLLVGVAGSSPADLVEPALEAFTSGGKRLRPLLLVLSARMGEPRHEDLLGAATAI
                     EVLHTATLIHDDIVDRAESRRGVPTTAAGYGREIAAATGDYLFAEAFSELARIGDPRL
                     IRAFAEASVGLAAGELGQYRANGATVDVEAYLEHIRKKTAGLFQAACVAGGTLGGLSL
                     VQIDALASYGQALGIAFQMSDDIMDLVGKPGLMGKGIGTDLVEGTVTLPVIFALGEGD
                     AETIRRVLADPSPPPELIEAGIEAVLATDAVARTEEWARGEIDAALEDLDLLPVSPER
                     EFLESVASEVVGRDV"
     gene            1570..>2749
                     /locus_tag="VNT12_05780"
     CDS             1570..>2749
                     /locus_tag="VNT12_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI45661.1"
                     /translation="MSESRTGPVIFAAGTLISVSSPPLRDGALLVRDGRIEDVGAIAD
                     VGRENPDTEVRFFPNYTIIPGAVNVHAHLGFRRKDRPEGITFSDWLGALIERLPEKEA
                     WTAEAASDCAREAIEAGTTYMAESSPYGECLPQLAESGMAGTVYAEFFPHEIGTPDEA
                     VEFIVAKVRKLREGLPERVDAQVSVHSPYTVDPESSRLAARRTREMGWRLAIHLSESP
                     EEVEFLKRGTGGLADIFGANEWGGVGVSPVRYAQSIELLAPETIAAHLATGFSQEDIA
                     VLAQTGVAVALCPRSNDFLGCGVSPVPGMIARGIRAGIGKDGLWSSPSMNLFEETLFA
                     LRLYGLSGAAGLELATLGGARALGIEAETGTLEVGKWADLAVVETSQENGDTERAVLE
                     A"
BASE COUNT          493 a          832 c          967 g          457 t
ORIGIN      
        1 actccaccac ctctggaaca gaaaggtggt cagggcaacg gggctgggga ccggcggcag
       61 ggcgctcgac ctcgcctgcg gcacggggac cctcacgtgc gatctcgcgg agaaggtcgg
      121 cgcggaaggt tacgttttgg gcgtcgactt ctccagggag atgctgcacg cagcacgctc
      181 ggcgcccaac gtcgagtacc ggctcgggga cgcgaccgat ctccagagtg tgccttccgg
      241 cgccttcgac gcggccacca tagcttacgg ggcgaggaac atccccgacc tcgacgcgct
      301 cttctccgag atggcgcgta cactcaggcc tggcggcgtc gcggtgtgcc tggagatagc
      361 gcggccaacg ggcaggatct cctccgcgtt ctacggggtg tggttcgata agatcgttcc
      421 aagactcgga ggttggatct ccggtgaccc atcagcttac gcttatcttc ccgaatccgt
      481 gaaagagttc gtggcgcccg gcgaattagc tgacataata gggcgtaatg gattacaaaa
      541 cgttacatgg gagcgcgtcg caggcggcat catcacgctc catcgtggga tcaggccaaa
      601 cgacgcaggc tagcaggctc cattccgcgc tgcccgaggg cgccttcgag gtcatcgacg
      661 aggtcgaggg tctgctcgtc ggggtggcag ggagttcccc cgcggatctg gtcgagccgg
      721 ccctcgaggc ctttacgtca ggggggaagc gtctcaggcc gctgctcctg gtgttgagcg
      781 cccgcatggg ggagcccagg cacgaggacc tgctcggggc ggcgaccgcc atagaggtgc
      841 tccacacggc caccctgatc cacgacgata tcgtggaccg ggccgagagt aggcgcggcg
      901 tgccgaccac cgccgccgga tacgggcgtg aaatcgcggc agccacggga gactacctgt
      961 tcgccgaggc tttttcggag ctggcgagaa tcggagaccc gaggctcatc cgggccttcg
     1021 ccgaggcctc cgtgggactc gccgcggggg aactcgggca gtaccgcgca aacggggcga
     1081 cggtcgacgt tgaggcgtac ctggaacaca taaggaaaaa gaccgcgggt ctcttccagg
     1141 ccgcttgtgt ggccggtgga acactgggcg ggctttcgct cgtgcagata gacgcgctcg
     1201 ccagctacgg ccaggctctg ggcatagcct tccagatgtc ggacgacata atggacctcg
     1261 tcggcaagcc ggggcttatg ggcaagggca taggcacgga cctcgtcgag ggcactgtga
     1321 cgctgccggt catcttcgcc ctgggcgaag gggatgctga gacgatccgg cgcgtcctcg
     1381 cagaccccag tccgccaccg gagctgatcg aggcaggcat agaagccgtg ctcgccacgg
     1441 acgctgtggc caggaccgag gagtgggcca ggggtgagat cgacgcggcc ctcgaagacc
     1501 tcgacctcct gcccgttagc cccgaacgcg agttcctgga gtccgtggcc tcggaggtgg
     1561 tcgggaggga tgtctgagag ccgtacggga cctgtgatct tcgcggccgg aaccctgata
     1621 tccgtctcgt cgccccctct gcgggacggt gccctgctcg taagagacgg gcgtatagag
     1681 gacgtaggag ccatcgcaga cgtcggacgc gagaatcccg acaccgaggt caggttcttc
     1741 cccaactaca cgatcattcc tggcgcggtc aacgtccacg cccacctcgg cttcaggcgg
     1801 aaggataggc cggagggcat cacgttctct gactggttgg gcgctctcat agagcgcctc
     1861 cccgagaaag aggcctggac ggccgaagca gccagcgact gtgcaaggga agccatagaa
     1921 gcagggacga cgtatatggc cgagtcatcc ccttatggag aatgcctgcc gcagctcgcc
     1981 gaaagcggga tggcggggac cgtctacgcg gagttcttcc cgcatgagat cggcacccct
     2041 gatgaggccg tcgagtttat agtcgcgaag gtgcgcaagc ttcgagaagg tctgccggag
     2101 cgtgtggacg cgcaggtaag cgtacattcc ccttacacgg tggaccccga gtcctcgcgt
     2161 ctggctgcgc gcaggacgcg ggagatgggg tggaggcttg cgatccacct ctccgagagc
     2221 cccgaggagg tcgaattcct aaagcgtggt actggcggtc tcgctgatat cttcggcgcc
     2281 aacgagtggg gtggggtggg cgtctcgccc gtccgctacg cgcagagcat agagcttctc
     2341 gccccggaga cgatagccgc gcacctggcg acgggattct cccaggagga catagcggtt
     2401 ctggcgcaga ccggtgtggc ggtggcgctc tgcccccgtt cgaacgactt cctcgggtgt
     2461 ggcgtctcgc ccgtacccgg catgatcgcc cgtggcatca gggccggtat cggaaaagac
     2521 gggctctgga gcagcccgag catgaacctc ttcgaggaga cgctgttcgc gctgaggttg
     2581 tacggtctga gcggcgcggc aggcctcgag ctggcaacgc tgggaggggc gcgggcgctc
     2641 ggtatcgagg ccgaaacggg gacgctcgag gtggggaagt gggccgacct cgccgtggtc
     2701 gagacctcgc aggagaacgg agatacagag cgggctgtac tcgaagctg
//
LOCUS       DATMJA010000244         4176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_211278, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000244 DATMJA010000000
VERSION     DATMJA010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211278"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VNT12_05785"
     CDS             <1..125
                     /locus_tag="VNT12_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal protein L16"
                     /protein_id="HWI45662.1"
                     /translation="GRVMFEMSGVDETLAREAMHLAAQKLPIKTRFLARGGGQQ"
     gene            122..313
                     /gene="rpmC"
                     /locus_tag="VNT12_05790"
     CDS             122..313
                     /gene="rpmC"
                     /locus_tag="VNT12_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWI45663.1"
                     /translation="MKVAEVRELDVEELEQRLAETRRELFNLRFQHATGQLENTGQLK
                     EVRKNIARILTVLNQKAGE"
     gene            461..727
                     /gene="rpsQ"
                     /locus_tag="VNT12_05795"
     CDS             461..727
                     /gene="rpsQ"
                     /locus_tag="VNT12_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWI45664.1"
                     /translation="MQTDTYRNRRKERVGLIVSDRADKTVTVSVEALVRHPMYKKRVR
                     RSRKFMVHDERNEARVGDTVRIVETRPLSARKRWRLANIISRAE"
     gene            727..1095
                     /gene="rplN"
                     /locus_tag="VNT12_05800"
     CDS             727..1095
                     /gene="rplN"
                     /locus_tag="VNT12_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWI45665.1"
                     /translation="MIQQESRLRVADNSGARSILTIRVLGGSKRRNAGVGDVIVATVK
                     EATPGSAVKKGDVVRAVVVRTRKETRRDDGSYIRFGENAGVIINPDGAPRGTRIFGPV
                     ARELREKGYTRIISLAPEVL"
     gene            1098..1415
                     /gene="rplX"
                     /locus_tag="VNT12_05805"
     CDS             1098..1415
                     /gene="rplX"
                     /locus_tag="VNT12_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWI45666.1"
                     /translation="MGLKLKRGDTVVVISGKEKGKRGEIERVIPKENRVVVGGVNVRT
                     RHARPSQQNQQGLYHFEAPIDVSNVMLVDPNSGEPTRVGYRFTDLGEKLRVGKKSGKD
                     IDG"
     gene            1408..1950
                     /gene="rplE"
                     /locus_tag="VNT12_05810"
     CDS             1408..1950
                     /gene="rplE"
                     /locus_tag="VNT12_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWI45667.1"
                     /translation="MARLKERYNEEVVADLQERFGIENPMRMPGLEKIVLNMGVGEAV
                     VNSRALDGAMEDLARITGQKAQLRRARKSIAGFKIREGMPVGARVTLRGERMWEFLDR
                     FISIALPRVRDFRGVSPASFDGRGNYAIGLKEQVIFPEISYDSIDATRGLDVAIVTTT
                     ESDEEARELLRLLGMPFRQS"
     gene            1961..2146
                     /locus_tag="VNT12_05815"
     CDS             1961..2146
                     /locus_tag="VNT12_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWI45668.1"
                     /translation="MTRKALLVKQQKKQKYPVREYNRCQRCGRARGYYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            2153..2551
                     /gene="rpsH"
                     /locus_tag="VNT12_05820"
     CDS             2153..2551
                     /gene="rpsH"
                     /locus_tag="VNT12_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWI45669.1"
                     /translation="MSVNDPIADFLTRIRNAHMAKQETVEIPHSKMKAEISRILLEEG
                     YVQDVSERGSGVERRLVITLKYGPDGSRGIAGLRRMSRPGRRVYRKQTNIPRVLDGLG
                     VAILSTSQGVLTDHQARRRGVGGEVLCFVY"
     gene            2566..3099
                     /gene="rplF"
                     /locus_tag="VNT12_05825"
     CDS             2566..3099
                     /gene="rplF"
                     /locus_tag="VNT12_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWI45670.1"
                     /translation="MSRIGKAPVEIPGAVSVELSGRSVKVNGPKGELTVPVGRGIDVR
                     QEDGNVIVERAADTPEHRAMHGLTRSLIYNAVTGVTEGFTKTLNIAGVGYRAALQGSD
                     INLQVGYSHPVSISPREGIEFEVPNATTIIVRGIDKQRVGQTAAEIRMVRPPEPYKGK
                     GIRYSDEQIRRKVGKAG"
     gene            3114..3464
                     /gene="rplR"
                     /locus_tag="VNT12_05830"
     CDS             3114..3464
                     /gene="rplR"
                     /locus_tag="VNT12_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWI45671.1"
                     /translation="MAVLEKRMHREKRHKRVRRKVAGTASRPRLSVYRSNVHIYAQLI
                     DDDAGETLAAADSRQVGESENRKDAARKVGALIAKEASQAGIEAVVFDRGGNKYHGRI
                     AALAEGAREGGLRL"
     gene            3484..4017
                     /gene="rpsE"
                     /locus_tag="VNT12_05835"
     CDS             3484..4017
                     /gene="rpsE"
                     /locus_tag="VNT12_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWI45672.1"
                     /translation="MGRPRTDNRQGGGRGGRQRDGASDLQDRVVEIRRVAKVVKGGRR
                     FNFSALVVVGDGKGRVGTGLGKANTVPAAIQKGQERAKRNMFDVPMRNTTIPHEVTGK
                     FESSLVLLKPASEGTGVIAGGGVRAVLELAGIKDVLTKALGSTTSVNLVQATVEGLQR
                     LRSKADIEQTRGVKITL"
     gene            4014..>4176
                     /gene="rpmD"
                     /locus_tag="VNT12_05840"
     CDS             4014..>4176
                     /gene="rpmD"
                     /locus_tag="VNT12_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWI45673.1"
                     /translation="MSPLKVTQVRSVVGSKQGHKRTVRALGLKRIRDSRVHEDTPQIR
                     GMVHKVQHLV"
BASE COUNT          919 a         1193 c         1463 g          601 t
ORIGIN      
        1 ccgggcgtgt gatgttcgag atgagcggtg tggacgagac gctggcccgc gaggccatgc
       61 acctcgccgc ccagaagctc cccataaaga cgcgcttcct tgcgcgaggg ggtggtcagc
      121 agtgaaggta gccgaagtgc gcgagctgga cgtcgaggag ctagagcagc gcctcgccga
      181 gacgcggcgc gagctcttca acctgcgctt ccagcacgca accgggcagt tggagaacac
      241 gggacagctc aaagaggtca ggaagaacat cgccaggatc ctgacggttc tgaaccagaa
      301 agcaggagag taggtagtgg tagaggaaga gaaaaaccag actcctgaga cggccgagga
      361 gcccgtggcc gagacgccac aggacctcat cgaggcgggc gaggccggtc tcggtgcggg
      421 tcctgccagg ggcccggcgc tggaccagga tgagggccag atccagaccg acacctacag
      481 gaacaggcgc aaggagcggg tcgggctcat cgtctcggac agggcagata agacggtgac
      541 cgtctctgta gaggcgctgg tgcgccaccc gatgtacaag aaacgggtgc gccgttccag
      601 gaagttcatg gtgcacgacg agcgcaatga ggcccgcgtg ggcgataccg tacggatcgt
      661 agagaccagg ccgctctcgg cccgcaagag gtggcggctc gcgaacatca tttcgagggc
      721 cgagtagtga tacagcaaga gtcgagactc agggtcgcgg acaactcggg tgccaggagc
      781 atcctgacga tccgcgtgct cggcggaagc aaacggcgca acgccggcgt cggggacgtg
      841 atcgtggcca ccgtcaagga ggccacgcct ggcagcgccg tcaagaaggg cgacgtggta
      901 agggccgtcg tggttcggac caggaaagag acgcgccgcg acgacgggtc atacatccgc
      961 tttggggaga acgcgggcgt gatcatcaac cccgatggcg ctccccgcgg gacgcgtatc
     1021 ttcgggcccg ttgcgcgcga actcagggag aagggttaca ccaggatcat ctcgctcgct
     1081 ccggaggtgt tgtagaaatg ggtctaaagc taaagcgcgg ggacacggtc gttgtgatct
     1141 cgggcaaaga gaagggcaaa cgcggcgaga tcgagcgggt catcccgaaa gagaacaggg
     1201 tcgtcgtcgg tggcgtcaac gtccgcacgc gccacgcgag gccgagccag cagaatcagc
     1261 aagggctcta ccacttcgag gctcccatag acgtctccaa cgtgatgctc gtcgacccga
     1321 actcgggcga gccgacgagg gtgggctacc gctttacgga cttgggggag aagctccggg
     1381 tcggaaagaa gtcagggaag gatatcgatg gctaggctga aggaaaggta caacgaggag
     1441 gtggtcgcgg atctccagga gaggttcggc atcgagaacc ccatgcgtat gcctggcctg
     1501 gagaagatcg tgttgaacat gggagtcggg gaggccgtgg taaacagccg cgccctcgac
     1561 ggggcgatgg aggacctcgc caggatcacc ggccagaagg cccagctcag gcgggcgcgc
     1621 aagagcatcg ccgggttcaa gatcagggag ggcatgcccg tcggggccag ggtaacgctg
     1681 cgcggcgagc gcatgtggga gttcctcgac cgttttatct ccatagccct accgcgcgtc
     1741 agagacttca ggggcgttag ccctgcctcc ttcgacgggc gtggaaacta cgcgataggc
     1801 ctcaaggagc aggtcatctt ccccgagatc tcctacgact ccatagacgc gaccaggggt
     1861 ctcgacgtgg cgatcgtgac gacgacggag agcgacgaag aggcgcggga gctcttgcgg
     1921 ctcctcggga tgccgttcag gcagagttga gaggtaggaa atgacgagaa aggcgttgct
     1981 ggtaaagcag cagaaaaaac agaagtaccc ggtcagggag tacaaccgtt gccagaggtg
     2041 cggccgggcg cgcggctact acaggaagtt cggcctttgc cggatctgcc tcagggagct
     2101 ggcgcacgag gggaagatcc ctggcgtaac gaaagcgagc tggtagataa ctatgtcggt
     2161 taacgatcca atagcggatt tcctgacgcg catccgcaac gcgcatatgg ccaagcagga
     2221 gacggtcgag ataccgcact cgaagatgaa ggcggagatc tcccgaatcc ttctggagga
     2281 aggctacgtt caagacgtct ccgagcgcgg aagcggggtc gaaaggaggc tcgtaataac
     2341 cttgaagtac ggccccgacg gtagccgcgg catcgccggc ctgaggcgga tgagccgccc
     2401 cggacgccgg gtctaccgca agcagaccaa catcccccgc gtgctcgacg ggctcggggt
     2461 ggcgatactc tcgacctccc agggggtcct gacagaccac caggccaggc gcaggggcgt
     2521 cggcggcgaa gtcctgtgct tcgtctacta gggaggtatt gaggagtgtc gagaatcggg
     2581 aaggctcctg tcgagatccc tggcgcggtc agcgtggagc tctcgggccg tagcgtaaag
     2641 gtgaacggcc cgaagggcga gctcacggtg cccgtggggc gcgggatcga tgtgaggcag
     2701 gaagatggta acgtaatagt cgagcgggcc gcggacacgc ccgagcaccg ggcgatgcac
     2761 gggcttacgc gatccctgat ctacaacgct gtaacgggcg tgacggaagg gttcacgaag
     2821 acgctgaaca tcgccggcgt cggctacaga gcagccctgc agggaagcga catcaatctg
     2881 caggtcggct actctcatcc tgtctccatc tcacctcgcg aggggataga gttcgaggtt
     2941 ccgaacgcca cgacgatcat cgtgcgcggg atagacaagc agagagtagg ccagacggcg
     3001 gcggagatcc ggatggtccg tccgcccgag ccctacaagg gcaagggcat ccgttacagc
     3061 gacgagcaga tccgtcgcaa ggtcggcaaa gccggatagt aggaggagga ttcatggctg
     3121 ttctcgaaaa gcggatgcac agggagaagc ggcacaagcg tgtcagacgc aaggtggccg
     3181 gtacggcctc gaggccgcgc ctctccgtgt accgctccaa cgtgcacatc tacgcccagc
     3241 taatcgacga cgacgcggga gagacgctcg cggcggccga ctcccgccag gtaggggagt
     3301 ccgagaaccg caaagacgca gcccgcaagg tcggagcctt gatcgcgaaa gaagcctccc
     3361 aggccgggat agaggcggtc gtcttcgaca ggggcggaaa caaataccac ggacgcatcg
     3421 cggcgctcgc cgagggcgcg cgcgagggcg gactgaggct ctaactcttt aggaggaact
     3481 atcttgggac gaccaagaac cgataacaga caggggggag gacgcggcgg ccggcaacgc
     3541 gacggggcct cggatctcca ggacagggtc gtcgagatcc ggcgcgtcgc caaggtcgtc
     3601 aagggcggcc ggcgcttcaa cttctcggcc ctcgtggtcg ttggcgacgg caagggcagg
     3661 gtcggcacag gcctcggtaa ggccaacacc gtccccgccg ccatccagaa gggccaggag
     3721 cgggccaagc gaaacatgtt cgacgtaccg atgcgcaata ccaccatccc gcacgaggtc
     3781 acaggcaagt tcgagagctc tttggtgctc ctcaagccag cctcagaggg tactggtgtg
     3841 atcgccggcg gcggtgtcag ggctgtcctc gagctcgccg gcataaagga cgtactcacc
     3901 aaggcgctcg gctcgacgac ctccgtgaac ctcgtgcagg ccactgtcga agggctccag
     3961 aggctgcgca gcaaggccga catcgagcag acacgagggg tgaagatcac actatgagcc
     4021 cactcaaagt cacgcaggta aggagcgtcg taggctccaa gcagggtcac aagcggacgg
     4081 tgcgcgccct gggtctcaag cgcatcaggg actccagggt gcacgaggac acgccccaga
     4141 tccggggtat ggtccacaag gtgcagcacc tcgtcc
//
LOCUS       DATMJA010000245        11263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_566479, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000245 DATMJA010000000
VERSION     DATMJA010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566479"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VNT12_05845"
     CDS             <1..417
                     /locus_tag="VNT12_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWI45674.1"
                     /translation="HETVQETMPLDDRLHFDAAADFTARLVKVAFKGLPSGRILNVNA
                     PNLPSDRLRGARVTRLGRRFYKDELVEVRDEEGRVGYDIYNNPPGHHEEEGTDFAAIE
                     NDEISITPVHLELTDTSGLEELGAWDVDGLLRNGLD"
     gene            421..1068
                     /locus_tag="VNT12_05850"
     CDS             421..1068
                     /locus_tag="VNT12_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWI45675.1"
                     /translation="MLSAVLFDFDGTLVDTTEMIHQSMRHATSSVLGRDDIPRETLLA
                     NVGQPLPRQMELIDTENAESLLEAYRSHHEQHHDALIGEFPGVEESLARLGSAGIKVA
                     VVTSKRRLSVEMALKNFPGLRDVVDRFVTLEDTTEHKPHPEPLLRGLELLGSIPKERA
                     AYVGDSPFDVEAAKAAGLTSVAVSWGAFSDGALRAAEPDHLVPDIQSAVDVLLTL"
     gene            complement(1091..2200)
                     /locus_tag="VNT12_05855"
     CDS             complement(1091..2200)
                     /locus_tag="VNT12_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWI45676.1"
                     /translation="MFPALATNDCAYLNTGSSGPPPDYVLEAMREADDLCSGPAYLEG
                     FGLFVHQAEMNSRAREAAARLVGAGEVALTQNTTHGMNLGVGSIDWREGDEVVSVTTE
                     HPGCLVPLHNLKDRFGAKIDLISPPVTPEKIEASLTPNTRLVALSHVDWTNGEVLPLR
                     EICAVAREREVLTLVDGAQSVGNIPVDVPATGADMYAFTGHKWVLGPEGIGALYVRPG
                     LPVYSPNVGLMSLPDPADFDIEGGYGLRSDARRFEASTMSPALAGGLAAAANAVYERG
                     IAGFEEIQHRADLLMDLLSELPRVTIRSPRPAQSGLVSFEVEGVAAEGAVERLLEQGF
                     ILRYLPDPNPYVRASTHLFNTEEELEALAKTVRAL"
     gene            2279..4498
                     /locus_tag="VNT12_05860"
     CDS             2279..4498
                     /locus_tag="VNT12_05860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWI45677.1"
                     /translation="MQNGNLVRIGRRRRRARKKSKLLQRLRTALLLLCFVSILGVVGM
                     VASAVGTYQALAANMPDLDDYHSTELAQTSLIYDTNGNIVDQLYGVQNRFVVPLEEVD
                     PTLRQAIISIEDHRFYQHRGLDFEAIGRAAVENVKSLSIQEGGSTLTQQLIKNTYIAQ
                     QERQVPSFERKFVEGSLAWQYEKEHSKDEILEQYLNTVYFGSNAYGAEAAAKTYFNKQ
                     ASDLTLSESALLAGIINLPGVYDPFVDPESAKARRNVVLDRMLEYGHISKKEHDRAVA
                     SKLNVSRGRVQYQNDNEYFLNAVRKEIAEEYGDDMVYEGGLKIYTTLDPDLQAMANDA
                     VGSIVNPEAGDPSASLVSIEPGTGAVRAMVGGSDFDQVKFNLATQAHRQAGSSFKPFV
                     YSEAIHEGISPETMYVSKHLTIDMGQNAKPYEVDNYDFIHRGPITLEKALADSDNTVF
                     VQLALDLGLQKVVEMANELGIDTEIDAYPSTAIGGLRVGVTPLEMASAYSTFANSGTH
                     MEPYLVEKLTKDDKGKDTLLEEHRPFGEEVLSRNEAAVVTEALRGVTERGTASYYHDL
                     EAEIGRPAAGKTGTSNEFVDAWFIGFIPQLSTSVWVGYPNERRSMVNINGLPQVNGEN
                     YPLDIWSLYMQSAVQKYPIRQFDAPNPELHLEVMTDGRTYVKPEPPPEPEKPKEEKST
                     VTKKEDPGPENKGTKPEEPRSFEEQPPSAPDSNQEPTSQQAPASASPASAPPEPDGG"
     gene            4577..5737
                     /locus_tag="VNT12_05865"
     CDS             4577..5737
                     /locus_tag="VNT12_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI45678.1"
                     /translation="MKQAGFARDSATWMTYGLVGFFAFMETVLGPIMPFLRRELDLGY
                     AAASLHFSAFALGAVLLGFFGDRLVGRWGRRAALWGGAFGVAGGALFLISSPSAWGTV
                     PATFAMGICGALVLVTSQAVLSDRHGEYSAVALTESNVTASACAIVAPLLVGASAASG
                     LGWRAALVLPVVALVMLAARFFSRTLDLPPNAVEDDAPADGPTLPPLYWAFWTLVALG
                     VASEWCVGYWGADFLADGTGLTRPAAATSLTVFFAAMLVGRIASSRLARTLPAAVLLA
                     VTLFVALLGFPLFWLSPGTLLALAGLFLTGLGIGGVYPLGVSAAIAAAPGNTDAAAAR
                     LAIGGGGAILVAPFVLGALADRVGIGTAFGIVVPMLLAALSLALIAGRRGRQ"
     gene            complement(5802..6599)
                     /locus_tag="VNT12_05870"
     CDS             complement(5802..6599)
                     /locus_tag="VNT12_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HWI45679.1"
                     /translation="MGTTKDTPEELHAFVCDVARRAGELQISRYHDPGEVREKAPKDF
                     VTEVDLLCEELMVNAIEERYPDDAILSEERGGSVSENGRTWLLDPVDGTANYMKANPM
                     FCACISLIEDGSVTHAAVAAPRLGDLYHARSGGGAYKETGGKEISLRVSDTERLEYAV
                     AGADLSLYGGKPQEPGIERVLYRAWQLRALGSAGIRGAWLAAGYLDVSVGTQNTAWDY
                     APTVLLVSEAGGRATDLSGEPWSLSSEGLIATNAALHDEVLETLHSA"
     gene            complement(6615..7718)
                     /locus_tag="VNT12_05875"
     CDS             complement(6615..7718)
                     /locus_tag="VNT12_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI45680.1"
                     /translation="MATQNSMMLAERTDELLSRGRSQGFLSTEEVANLLQEGDLSAAE
                     IEEFYATLEEEEITIVEGEASENGGSRTAVAATVHVSEPPAVQIAHAVATGDSIRMYL
                     AEIGRVPLLTHADEIRLAKGIARGCKRSKDRLVEANLRLVVSIAKKYRNRGVSFLDLI
                     QEGNLGLIRAAEKFDHSKGYKFSTYATWWIRQAITRAIADKGRTIRIPVHMVEKVNKY
                     HRTHRRMIQALGREPSEEEIALELGVFVAEIQRLQEISQRSISLATPVGDDDSSELGD
                     FLEDAGSVTPTDAVSESLLKAHLREALDELPERERQIIELRFGMKDDRPRTLEEVGRE
                     FDITRERVRQIQMKTLNLLREQRRTQNLREYLN"
     gene            8021..9484
                     /locus_tag="VNT12_05880"
     CDS             8021..9484
                     /locus_tag="VNT12_05880"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWI45681.1"
                     /translation="MILDRAKLTGVLGTEIPSGMRGVRGVTHDSRCVEPGFAFVAIPG
                     FKRDGTEFAPEAVRRGAVLVVAESDVPGVPTAIVADARGALAVLAREVNGDPSRSMEV
                     YGVTGTNGKTTTSYVLHAVLAGAYGEGECGLMTTAETISGGEIRPAVRTTPEATEVQG
                     TLASMLRSGVRKVVMEISSHGVSLKRIAGTRFSGAIFTNLTRDHLDLHGSMEAYYAAK
                     RELFYMTEGPRLANAEDAYGRRLAGELESVSTFGSTAKADYRIADVETTRSGTSLALH
                     HSEDVLRLKTPLLGPYNVLNVAGAVSLALAVGTDKTALRRAVREMGQVPGRFERVAAA
                     GEYGFEIVVDYAHTDVGLDAVLRVARGVAGSRGRVICVFGAAGDRDGAKRPKMGRVAS
                     LLADMSFITTDDAYSEDPEKIATEVEAGSDLSRTSIELDRRKAIRRALEAARAGDVVV
                     VAGKGHERVQHLPEGDIPFHDATVVRELLAEIGDRGR"
     gene            9493..9753
                     /locus_tag="VNT12_05885"
     CDS             9493..9753
                     /locus_tag="VNT12_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWI45682.1"
                     /translation="MQKQTKSEVLRRLKSVEGHVRGVEKMVEEGDVTYADIVQQTNAI
                     FSAIRRINTVLLKDFVEERAERDGASEEMISDLIKAIDRVTK"
     gene            9811..10179
                     /locus_tag="VNT12_05890"
     CDS             9811..10179
                     /locus_tag="VNT12_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45683.1"
                     /translation="MVGTSAGLPGAEGEVGMVYSPEPMEDRGKQQDDLGGLEEKIDEL
                     EKLTDSLDDVPEEDLVGTLGEAVELLAEINAGIEARLDTAGDESREIGALLGQVDFGP
                     FDEAMEDHEHRERTTGEPGA"
     gene            10163..10696
                     /gene="pyrE"
                     /locus_tag="VNT12_05895"
     CDS             10163..10696
                     /gene="pyrE"
                     /locus_tag="VNT12_05895"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWI45684.1"
                     /translation="MSRVPDLAPRIRDAALLEGDFVLSSGERSSFYVDKYLFSTEPNL
                     LRDVADALAAEIPGGVERLAGVELGAVPLVVATALATGLPYVIVRKSAKEHGSSAGRS
                     IEGNLYQGERVALVEDVVTTGTQAVRAARNLEETGAEVVTIVAVLDRREENGGKLGGF
                     PFRSLLRMNDLRVVKRN"
     gene            10773..11051
                     /locus_tag="VNT12_05900"
     CDS             10773..11051
                     /locus_tag="VNT12_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWI45685.1"
                     /translation="MALNKTALIEEIAGKANASKSDAQRFFDAFTEVVTDSLKNGEQV
                     QITGFGKFYVQKRDARQGINPQTKQRINIPASKVPKFTAGNALKDQIK"
BASE COUNT         2127 a         3493 c         3606 g         2037 t
ORIGIN      
        1 catgagacgg tccaggagac catgccactc gacgataggc tccacttcga cgccgctgcg
       61 gattttacgg cacgcctggt gaaggtagcg ttcaagggtc ttccgtcggg acgaatcctc
      121 aacgtcaacg cgccgaacct gcccagcgat aggctccgag gcgcgcgggt caccagactc
      181 gggcgccggt tctacaagga cgagctcgtc gaggtaaggg acgaagaggg gagggtcggc
      241 tacgacatct acaacaaccc tcccggccat catgaggagg agggcacgga cttcgctgcc
      301 atcgagaacg atgagatcag catcaccccc gtccacctcg agctgaccga tacctctggc
      361 ctcgaggaac tcggcgcctg ggacgttgat ggcctgctca ggaacgggct cgattagaag
      421 ttgctctccg cggtcctctt cgacttcgac gggacgctcg tcgatacgac ggagatgatc
      481 caccagtcca tgcgccacgc cacaagctcg gtcctcggcc gcgacgatat cccccgcgag
      541 acgctgctgg ccaacgtggg ccagcccctc ccgcgccaga tggagctgat agacacagaa
      601 aacgccgagt cgctactcga ggcctaccgc agccaccacg aacaacacca cgacgccctg
      661 atcggggagt tccccggtgt cgaggagtca ctcgctcggc tcggatcggc cgggataaag
      721 gtcgccgtgg tcacctccaa acgccgcctc tcggtggaga tggccctgaa gaactttccc
      781 ggtctccgcg acgtcgtgga ccgcttcgtc acgctggagg acactacaga gcacaagcct
      841 caccccgagc cattgttgag aggactggag ctgctcggca gcatcccgaa agagcgagcc
      901 gcctacgtag gcgactcccc cttcgacgtg gaagcggcga aggccgcggg cctcacgagc
      961 gtcgccgtaa gctggggcgc cttctccgat ggtgccttac gcgccgcaga acccgaccac
     1021 ctcgtccccg acatccaatc ggcggtcgat gtcttattga cgttgtaggg tcgttcgcga
     1081 acgaccccta ttacaaagcc cgcacggttt ttgccaacgc ctccaattcc tcctccgtat
     1141 tgaacaggtg cgtgctcgcc ctgacgtagg ggttcggatc cggcaggtag cgaaggatga
     1201 aaccctgctc caacagacgc tcgacggcgc cttcggccgc cacgccttcg acctcgaagc
     1261 tgacgagccc ggattgggct ggtctcggtg aacgtatggt aactcgcggt agctctgaga
     1321 ggaggtccat caggaggtcg gcgcggtgct gaatctcttc gaatccggca atgccgcgct
     1381 cgtagacggc gtttgctgcg gcagcgagac cgcctgcgag ggcggggctc atggtcgagg
     1441 cttcgaagcg gcgggcgtcg gatctcagcc cgtagccacc ctcgatgtcg aaatctgctg
     1501 ggtccggcag tgacataagg cctacattcg ggctatagac cggcaagcct ggcctgacgt
     1561 ataaggcgcc aataccttca ggcccgagca cccacttgtg gcctgtgaat gcgtacatgt
     1621 ccgctcctgt ggccggtaca tcgaccggaa tattcccgac cgattgggcg ccgtccacca
     1681 gggtgaggac ttccctctcc ctggccacgg cgcagatctc ccgcaacggc agtacctctc
     1741 cgttcgtcca gtccacgtgc gacagggcga cgaggcgggt attcggcgtc agggaggctt
     1801 cgatcttctc cggcgtcacg gggggcgaga tcaggtcgat cttcgctccg aagcggtcct
     1861 tcaggttgtg gagcggcacg aggcagcccg ggtgctccgt ggtgacagag acgacctcgt
     1921 cgccttcgcg ccagtctatc gagccgacgc cgaggttcat gccatgggtg gtgttctggg
     1981 tgagggccac ttccccagcc cctacgaggc gggccgcggc ctccctggcg cgtgagttca
     2041 tctctgcctg gtggacgaag aggccgaagc cttcgaggta ggcggggcct gagcagaggt
     2101 cgtccgcttc cctcatggcc tccagaacat agtcgggggg aggtccgctg ctgccggtgt
     2161 tcaggtaggc gcagtcgttc gtggcgaggg ccgggaacat ctcccggggg aggctcgtgt
     2221 ctgtaatggc gcttctcctt ccgggttcgg aatgcccatt ctgtataata gcctcttcat
     2281 gcagaacggt aacctggtca ggatcggccg caggcgcagg cgcgcgcgca agaagagcaa
     2341 gctcttgcag aggctaagga ctgcacttct gctgctctgc tttgtgagca tactcggggt
     2401 ggtcggcatg gtggcaagcg ccgtcggcac ctaccaggcc ctcgccgcca atatgccgga
     2461 cctcgacgac taccactcca ccgagctcgc ccagacctcc ctcatctacg acacaaacgg
     2521 caatatcgtc gaccagctct acggcgtcca gaaccgcttt gtggtccccc tcgaagaggt
     2581 cgacccgacc ctcaggcagg ccatcatctc catcgaagac caccgtttct accagcacag
     2641 ggggttggat ttcgaggcca tcgggcgcgc ggcggtggag aacgtcaaga gcctctccat
     2701 ccaggagggc ggctccaccc tgacccagca gctcattaag aacacctaca tagcccagca
     2761 ggagcgccag gttcccagct tcgaacggaa gttcgtcgaa ggctcgctcg cctggcagta
     2821 cgagaaggaa cactccaagg atgagatcct cgagcaatac ctcaacaccg tgtacttcgg
     2881 gtccaacgcc tacggcgctg aggccgcggc caaaacctac ttcaacaaac aagcctcaga
     2941 cctaacgctc tcagagtcgg cgcttctggc agggataatc aatctgcccg gcgtctacga
     3001 tcctttcgta gatcccgaga gcgcgaaagc gaggcgcaac gtcgtgctcg acaggatgct
     3061 cgagtacggc cacatctcca agaaagagca cgacagggct gtggcatcga agctgaacgt
     3121 gagtcgcggc agggtccagt accagaacga caacgagtac ttcctcaacg ccgtccgaaa
     3181 ggagatagcc gaggagtacg gcgacgacat ggtctacgag ggcgggctca agatctacac
     3241 caccttggac ccggatctcc aggctatggc gaatgatgca gtcggctcca tcgtcaaccc
     3301 ggaggctgga gatccttccg cctccctggt ctccatcgaa cctggaaccg gcgcagtaag
     3361 ggccatggtc ggcggctcgg acttcgacca ggtgaagttc aatctcgcga ctcaggcaca
     3421 ccggcaggcg ggaagctcgt tcaagccttt cgtctactcg gaggccatac acgagggtat
     3481 ctcacccgag accatgtatg tctccaagca cctcaccata gacatgggcc agaacgcaaa
     3541 gccctacgag gtggacaact acgacttcat ccaccgcggc ccgatcaccc tagagaaggc
     3601 gctcgccgac tcggacaaca cggtcttcgt ccagttggcc ctggatctcg gcctccagaa
     3661 ggtggtggag atggccaacg agctgggcat agacaccgag atcgacgcct atccttcgac
     3721 cgcgatcggg ggcctcaggg tcggggttac gcctctggag atggcctcgg catactcgac
     3781 cttcgccaac tcgggtacgc acatggaacc ttacctggta gagaagctca cgaaggacga
     3841 caaagggaag gacacgctcc tggaggaaca ccgccccttc ggcgaggagg ttctaagtag
     3901 gaacgaggcg gccgtcgtca cggaggcgct gcgcggcgtg acggaacgcg gaacggccag
     3961 ctactaccac gacctcgaag ccgagatagg ccgccccgcc gcgggcaaga caggcacctc
     4021 caacgagttc gtagacgcct ggtttatcgg cttcataccg caactctcaa cgagcgtctg
     4081 ggtaggctac ccgaacgaac ggcgctccat ggttaacata aacggcctcc ctcaggtcaa
     4141 cggcgagaac tacccgctgg atatctggtc cctgtacatg cagagcgccg tccagaagta
     4201 cccgatccga cagttcgacg ccccgaaccc cgaattgcac ctggaggtga tgaccgacgg
     4261 acgcacctac gtcaagcccg aacccccgcc agagccggag aagcccaaag aagagaagag
     4321 cacagtcacg aagaaagaag acccgggtcc agagaataaa ggtaccaagc ccgaagagcc
     4381 acgctccttc gaggagcagc caccatccgc gccagattcg aatcaggagc ccacatcgca
     4441 acaggccccg gcaagcgctt ctccagcatc ggcgcctccc gaaccggacg ggggctagaa
     4501 cgtacatggg gacagccagt ctcgagtctt cgccaggccg caagagcgat gtgagagtag
     4561 agatccggag tagttcgtga agcaggccgg cttcgcaagg gactctgcca cctggatgac
     4621 ttatggcctg gtcggcttct tcgcgtttat ggagacggtg ctggggccga tcatgccctt
     4681 cctcaggagg gagctggacc tcggctacgc cgcggcgagc ctccacttca gcgccttcgc
     4741 cctcggcgcc gtgttgctcg gcttcttcgg agacaggctc gtgggccgct ggggaagacg
     4801 cgcagcgctc tggggcgggg cattcggcgt ggccggcggc gccctattct tgatctcgag
     4861 cccgagcgcc tggggcaccg tccctgcgac cttcgccatg ggcatctgcg gcgccctggt
     4921 gctcgtaacc tcccaagcgg ttctctccga cagacacggg gagtacagcg ctgtagccct
     4981 caccgagtcc aacgtcacgg cgagcgcttg cgcgatcgtc gccccgctcc tggtcggcgc
     5041 gtcagcggca tcggggctcg gctggcgcgc ggcgctggtc ttgcccgtgg tggccctcgt
     5101 gatgctcgcc gcccgtttct tctcccggac cctggatctt ccgccgaacg cggtggagga
     5161 tgatgcgccg gccgatggcc cgactctgcc cccgctctac tgggcgttct ggacgctcgt
     5221 ggcgcttggc gtcgcctcgg agtggtgcgt cggatactgg ggggcggatt tcctcgcgga
     5281 cggaaccggc ctcacccgcc ccgccgcggc aacctccctg accgtcttct tcgccgcgat
     5341 gctcgttggc cggatcgcga gcagccgcct ggcgcgtacg ctgcccgcgg cggtcctcct
     5401 cgccgtgacc ctcttcgtgg cgctcctcgg gttcccgctg ttctggctct ctccggggac
     5461 tctcctggcc ctagccggcc tcttcctaac ggggctcggc atcggcggcg tctacccgct
     5521 tggcgtctcg gcggccatcg cggcggcgcc aggcaacacc gatgcggccg cggccaggct
     5581 cgctatcgga ggaggcggcg ccattctcgt cgctcccttc gtcctgggag ccctggccga
     5641 cagggtaggc ataggcaccg ccttcggcat cgtagtccca atgctcctcg cagccctgtc
     5701 gctggcactc atcgcgggac gaagaggacg ccaatagtcc aaggtcgccg ccgactcata
     5761 acggtagatg tttcgacgct gttacgcaga gtcgggcgcg ctcaggcgct gtgtagggtc
     5821 tccaggactt cgtcgtgcag ggcggcgttg gtggcgatga ggccttccga ggagaggctc
     5881 cacggctcgc ccgagaggtc tgtggcgcgt cctcctgctt cggagacgag gaggaccgtg
     5941 ggtgcgtagt cccaggccgt gttttgggtg ccgacggaga cgtcgaggta cccggcggcg
     6001 agccaggcgc ccctgatccc cgcgctgccg agagccctta gttgccaggc cctgtacagc
     6061 actctctcta tccctggctc ttgtggcttt ccgccgtaca gggagaggtc ggcgccggcg
     6121 acggcgtact ccagcctctc cgtgtcgctg acgcgcaggg atatctcttt gccaccggtc
     6181 tccttgtaag cgccgccccc ggacctcgcg tggtaaaggt cgccgaggcg gggcgcggcg
     6241 acggcggcgt gggtaactga gccgtcctcg atgagggaga tgcaggcgca gaacatgggg
     6301 tttgccttca tgtagttcgc cgtaccgtca acggggtcga ggagccaggt gcgtccgttc
     6361 tccgagaccg acccgccccg ctcctcggac aggatggcgt cgtcggggta gcgctcctct
     6421 atagcgttca ccatcagttc ttcgcaaagc aggtctacct cggttacgaa gtccttcggc
     6481 gccttctccc taacttcgcc agggtcatgg tagcgtgaga tctgaagctc ccccgccctg
     6541 cgggccacgt cacagacgaa cgcgtggagc tcttccgggg tgtccttggt ggtcccgatc
     6601 ttggtggagg ttctctagtt gaggtactcg cgcaggttct gggtgcggcg ttgctctcgg
     6661 agcaaattca gggtcttcat ctggatctgg cgtacccgct cgcgcgtgat gtcgaactcg
     6721 cggcctacct cttcgagggt gcggggccgg tcgtccttca tcccaaaccg gagctcgatg
     6781 atctgacgct ccctctcagg tagctcgtcg agggcttctc tgagatgggc cttgagcagc
     6841 gactccgaga cggcgtccgt gggggttact gagccggcgt cctccaggaa gtcgccgagc
     6901 tcggaggagt cgtcgtcccc gacgggcgtg gcaaggctta tggaccgctg gctgatctcc
     6961 tgcagcctct ggatctccgc cacgaaaacg cctagctcga gcgcgatctc ttcttctgaa
     7021 ggctcacgcc cgagggcctg gatcatgcgc ctgtgggtcc tgtggtactt gttgaccttc
     7081 tcgaccatgt gaacagggat gcggatggtc cgccccttgt cggcgatggc gcgggttatt
     7141 gcctgcctta tccaccacgt cgcatacgtc gaaaacttgt agcctttgga gtggtcgaac
     7201 ttctccgccg cccttatgag cccgaggttg ccctcctgga tgaggtcgag aaatgagacg
     7261 cctcggttgc ggtacttctt ggcgatggag acgaccaacc tcaggttggc ctcgacgaga
     7321 cggtctttgg acctcttgca ccccctggcg atacctttgg cgagccttat ctcgtcagcg
     7381 tgggtaagaa ggggcaccct tcctatctcc gcgaggtaca tcctgatgga atcccccgtg
     7441 gcaacggcgt gggcgatctg gaccgcagga ggctcagaga cgtggacagt ggcggccacc
     7501 gccgtccggc tccccccgtt ctcggacgcc tcaccctcca cgatcgttat ctcttcctct
     7561 tcgagcgtag cgtagaactc ctctatctcc gccgccgaga gatccccctc ctgcaagaga
     7621 ttggcgacct cctctgtgct gagaaagccc tgacttcgcc cccgactcag gagctcgtca
     7681 gtccgctccg ccagcatcat ggagttctgg gtcgccattt atctcctctc ctgcgcctct
     7741 gtgcacagac cgacagcagc caaacacaca tcgacacaat tgtgaaatcc atgtaaaaaa
     7801 tgacccgcgt agggtagcac accacatatg gtgagtcacc gtattttggc cctatttcgt
     7861 gtgtattctt ttaccacatt tccgctctcg gaaaaccggg gcgtgtttcg gtgtcgtttc
     7921 actgctgttt cagcggtatt tcaggcatgt ttcgccgtgt tgtgttcttt tgagaaaatc
     7981 tacgggaggc tctcgtattg ggtcttgcta aactagggcc atgatcctgg accgggcaaa
     8041 gcttaccggg gttctcggga cggagatccc ttccggcatg cgaggcgtga ggggagtgac
     8101 gcacgattcc cgttgcgtag agcctggctt cgccttcgtc gcgatccccg gcttcaagcg
     8161 tgacggaacg gagtttgcgc ccgaggccgt ccgtcgtggg gccgttctcg ttgtggctga
     8221 gagcgacgtt cctggtgttc cgaccgcgat cgtggcagat gcccggggtg ctctggcggt
     8281 gttggccagg gaggtcaatg gagatccttc gcggagcatg gaggtctacg gcgttactgg
     8341 gaccaacggc aagaccacga cctcctacgt cctgcatgct gtcctcgctg gagcctacgg
     8401 tgagggcgag tgcgggctga tgaccaccgc cgagacaatc tctgggggcg agatccgtcc
     8461 ggcggtacgg actacccccg aggccacaga ggtgcagggg acgctcgcct cgatgctccg
     8521 gtcaggcgtc cgcaaggtcg ttatggaaat ctcctcgcac ggcgtgtccc tgaagcgtat
     8581 cgccgggacg cgcttctcag gggctatctt caccaacctg accagggatc acctggatct
     8641 ccatggctcg atggaggcct actacgcagc caagcgggaa ctgttctata tgacggaagg
     8701 tcccaggctc gccaacgccg aggatgccta cgggaggcgg ctggccggcg agctggaaag
     8761 cgtgagtaca ttcgggagca cggctaaggc ggattacagg atcgccgacg tcgagacgac
     8821 caggagcggg acgagcctcg cgctccacca ttccgaggac gttctcaggc tcaagacgcc
     8881 gctgctcgga ccttacaacg tgctcaacgt cgctggggcc gtctccctcg cgctcgcagt
     8941 cgggacggat aaaacggcgc taagacgtgc ggtgcgggag atgggacagg ttcctggacg
     9001 cttcgagcgg gtcgcggcgg ccggggagta cgggttcgag attgtcgtag actacgccca
     9061 caccgacgtt gggctggatg ctgtcctgcg ggtggccagg ggggtagccg gtagcagggg
     9121 gcgggttatc tgtgtgttcg gggcggccgg agatagggat ggggcgaaac ggcccaagat
     9181 ggggcgggta gcctcgcttc tcgccgacat gagtttcatt acgacggacg acgcctactc
     9241 ggaggatcct gagaagatag caacagaggt cgaggccggc tcggatctct cacggaccag
     9301 catcgagctc gacaggcgga aggcgatccg gcgcgcgctg gaggcggcga gagccggtga
     9361 cgtcgtagtg gtagccggca agggacacga gagggtccag cacctccctg aaggggacat
     9421 cccattccac gacgccacgg tggtcaggga gctactggcg gagatagggg atagagggag
     9481 gtagcgcaag cgttgcagaa gcagactaag agcgaggtgc tacgccggct caagagcgtg
     9541 gaaggccatg tcaggggggt cgagaagatg gtcgaagagg gtgacgtaac ctacgccgac
     9601 atcgtccagc agactaacgc catcttctcg gctatcaggc gcataaacac ggtcctcttg
     9661 aaggacttcg tcgaggagcg ggccgagcga gatggcgcct ccgaagagat gataagtgac
     9721 ctgataaagg ccatagacag ggtgacgaag tgaaggtagg ggggcgggct atggtgtcct
     9781 tgaccgtgag cagggccgca aaggccagtc atggtaggaa cgagcgcggg tcttccgggg
     9841 gccgaaggcg aggtggggat ggtgtatagt ccggaaccaa tggaggatcg agggaagcaa
     9901 caggatgatc tgggaggcct ggaggagaag atcgacgagc tcgagaagct caccgactct
     9961 ctcgacgacg taccagaaga agatctggta gggaccttag gcgaggctgt tgagctattg
    10021 gccgagatca acgccggcat cgaagccagg ctcgacaccg cgggggacga gtcgcgcgag
    10081 atcggcgcgc tcctcgggca ggtagacttc ggtcccttcg acgaggccat ggaggatcac
    10141 gaacacaggg agaggactac cggtgagccg ggtgcctgat ctcgcacccc gcatcaggga
    10201 cgcggcgctc ctggaagggg acttcgtgct ctccagcgga gagcgcagca gcttctatgt
    10261 ggataagtac ctcttctcga ccgaaccgaa cctcctgcgc gacgtggccg acgccctggc
    10321 ggcagagata ccggggggag tagagcggct cgccggcgtg gagctcggtg ccgttcccct
    10381 cgttgttgcc acggccctcg ccacagggtt gccctacgta atcgtccgca agtctgccaa
    10441 agagcatggc agctccgccg gacgcagtat cgaaggcaac ctctaccagg gcgagagagt
    10501 agccctagtc gaggacgtcg tcaccacggg tacgcaggcc gtgcgggccg ccaggaacct
    10561 cgaagagacg ggggccgagg tcgtgacgat agtagcagta ctcgaccgca gggaggagaa
    10621 cggggggaag ctcggaggat ttcctttccg gtccttgctt agaatgaatg atttacgggt
    10681 agttaaacgg aattgaggcg ctatagtgtc tctggtagat ccttccggca ggccggaggg
    10741 aagaggagtg gaagcgaagc agggaggaag aagtggcgtt aaacaaaacg gcattgatag
    10801 aggagatcgc cggcaaggcg aacgcctcca agagcgacgc ccaaaggttc tttgacgcgt
    10861 tcaccgaggt cgtgacggac agcctcaaga acggcgagca ggtccagatc acgggcttcg
    10921 gcaagttcta cgttcagaag cgcgacgcca ggcaggggat caacccccag accaagcaga
    10981 ggatcaacat accagcatcg aaggtgccga agttcaccgc aggcaacgcg ctcaaggacc
    11041 agatcaagta gctgcccagg gtcttttctg gtgccgcggt gagaagcccg cggcaccatt
    11101 cttttcgccc gtttgtcgac caaccagggc acgatgcagg cgctggtaga cgcggcccgc
    11161 cgcaaggaga gcgtgctcgt cgccggcctc gaccccatac cctcacgcta tccagaagaa
    11221 ctgcgcgctc tgcccgaggc cgaagcgacc ctagagttct gcc
//
LOCUS       DATMJA010000246         5303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_803767, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000246 DATMJA010000000
VERSION     DATMJA010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803767"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VNT12_05905"
     CDS             <1..428
                     /locus_tag="VNT12_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HWI45686.1"
                     /translation="WHLLEETPHRGVQTLVKDLNALYSATPALYEVDFEPAGFGWIEG
                     GDMENSVVSFLRRGNKDFAIVVCNFTPVIRHGYRIGVPEGGAYEEALNTDDTRYGGSG
                     VRNSEGVVAEKVPAHGQPYSLSLTLPPLATMVLKKVSGL"
     gene            501..2648
                     /gene="glgX"
                     /locus_tag="VNT12_05910"
     CDS             501..2648
                     /gene="glgX"
                     /locus_tag="VNT12_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWI45687.1"
                     /translation="MASVVWPGHPYPLGATWDGQGVNFALFSENADRVELCLFDPSGK
                     EEIERIELRERTDRIWHCYLPYCRLGQLYGYRVHGPHDPENGHRFNPNKLLLDPYAKA
                     TVGALDWNYPHFGYTIGAEEEDLAYDERDNAAGMPKCRVVDTAFTWGEDRPPRTPWDE
                     TVIYELHVKGFTNRHPNIPEELRGTYAGLASAPAIEHLKRLGVTAVELMPVHSFVDDR
                     HLVDKGLKNYWGYNSIGYFAPDMRYSATGSVSEFKTMVKRLHSAGLEVILDVVYNHTA
                     EGNHLGPTLSFRGIDNTAYYRLVPDDPRYYMDFTGTGNTLNMMHPRVLQLIMDSLRYW
                     ILEMHVDGFRFDLASALARQLYEVDQLSSFFTIIHQDPVISQAKLIAEPWDVGEGGYQ
                     VGNFPIGWAEWNGKYRDVVRAYWKGEGGLVDELAYRLTGSSDLYEHGGRRPYASINFV
                     TAHDGFTLRDLVSYEEKHNEANGEDNGDGENHNLSHNYGVEGPTDDLEIRRIRARQRR
                     NLMATLLLSQGVPMILSGDEMGRTQEGNNNAYCQDNEISWLDWELSILDRNFLAFVRR
                     MIRLRKEHPVFRRRRFFQGRELRGAGAKDIIWLTPAGREMTDEEWNSSFARSLGLQMS
                     GLLEGEFDAQGRPEEDDDFLLLLNADQDEIIFELPPFPEEARWEVFMDTAYSAGLKTD
                     GFFKPGGTYTLKPRSMVVLVNARRRETVEEDAD"
     gene            2645..4417
                     /gene="treZ"
                     /locus_tag="VNT12_05915"
     CDS             2645..4417
                     /gene="treZ"
                     /locus_tag="VNT12_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HWI45688.1"
                     /translation="MRRHYMPFGAKVLEHGETRFALWAPAAETVDLILEDGVLLMHRE
                     EDGTFALTTESGAGARYRYRVDGGQEVPDPASRYQPDDVHGASRVVDPAAFVWREEHW
                     KGRPWEEVVLYELHVGSFTPEGTFAGIKEKLDYLVDLGVTAIELMPLSEFPGRRNWGY
                     DGVLPFAPESAYGAPEDLKDLVQTAHTKGLMVFLDVVYNHFGPEGNYLSLYAPQFFTD
                     RYQTPWGAAINFDGEGSKHVREFFIHNALYWLEEYGIDGLRLDAVHAINDASEKHFLV
                     ELAERVRQGPGRDRHVHLVLENEANDASYLRGHYDAQWNDDLHHALHVILTGESAGYY
                     ADYADAPMRRLGRSLGEGYAYQGEPSRHGKKRGEPSADLPSGAFVSFVQNHDQVGNRA
                     FGERIGQLAPPEAVRAAAEIYLLAPQIPMLFMGEEWASKTPFPFFCDFAPDLALLVAR
                     GRREEFSEFFDPEDDGRIPDPGAEETFRRAVLDWASLDEPEHSGRLKLYGELLALRRE
                     KIVPRLVGIPGDVAANQIVDDRGLSVQWTLADGSTLALLANLGPEPLDDPEWPPGERL
                     YASEDVTGHRQELPGWSVLWHLAE"
     gene            4399..>5303
                     /locus_tag="VNT12_05920"
     CDS             4399..>5303
                     /locus_tag="VNT12_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWI45689.1"
                     /translation="MAPGRMRIPRATYRLQLSEDFTFSDAAELVPYLSELGVSDLYAS
                     PYLKARPGSTHGYDVVDPGSLSPELGGEKEYGRLVEHLEEYEMGQLLDIVPNHMGVGS
                     DNAWWSDMLENGPASAYAAFFDIDWCPADRKELCGKVLLPILGDHYRAALERGEIRLA
                     FDAGEGSFSVNYYEHRYPIDPQTYPMILDRISLPEDDEHRQEIDGLIVAFGDLPGRDA
                     TGEGIVSGRSQNAANLKADLAALCGKSPGVLGAVEKHIREVNEAPADPRFKRLHRLLE
                     AQAYRLAYWRVGSDEINYRRFFAVN"
BASE COUNT         1076 a         1626 c         1650 g          951 t
ORIGIN      
        1 actggcacct gctagaggag acgccgcaca ggggcgttca aaccctcgta aaggatctca
       61 atgccctcta ctcggccacg ccagccctct acgaggtgga tttcgagcca gcaggtttcg
      121 ggtggatcga gggcggggac atggagaaca gcgtggtatc ctttctccgg cgtggaaata
      181 aggactttgc gatcgtcgtc tgcaacttta cgccggtcat acgccacggt taccggatag
      241 gtgtacccga agggggcgcc tacgaggagg ccctgaacac cgacgataca cgctacggcg
      301 gtagcggtgt gagaaatagc gaaggcgtgg ttgccgagaa ggttccagcg catgggcagc
      361 cttactcgct ctcgctgacg ttgcccccac tcgcgacgat ggttttgaag aaggtttcag
      421 gtctctgatt cactgctgaa gcctgatgcc tttagtctga aacctagaaa ggaatccatg
      481 ggaaataagg agatctctct ttggctagcg tagtctggcc tgggcatccg tatcctctgg
      541 gcgcaacctg ggatgggcag ggcgtcaact tcgccctgtt ctcggagaat gcagacaggg
      601 tggagctctg tctcttcgat ccttcgggaa aagaggagat cgagaggatc gagttgcggg
      661 aacgcacaga ccggatctgg cactgctacc tgccttactg ccgtctcggc cagctctacg
      721 gctacagggt ccacgggccc cacgaccccg aaaacggcca caggttcaac cctaacaagc
      781 tcctactcga cccctatgcc aaggcgactg tcggagccct agactggaac tacccgcact
      841 tcggatacac catcggcgcc gaagaggagg acctcgccta cgacgagcgg gataacgccg
      901 ccgggatgcc caagtgcagg gtggttgaca ccgcctttac ctggggtgag gacagaccgc
      961 cgcgcacgcc ctgggacgag accgtaatct acgagcttca cgtcaagggc ttcaccaaca
     1021 ggcatccgaa catccccgag gagttgcgcg gcacctacgc aggtctcgcc tcggcaccag
     1081 ccatagagca cctcaaaagg ctcggggtaa cggcggtcga gctcatgccc gtccactcct
     1141 tcgtggacga caggcatctc gtggacaagg gcttgaagaa ctactggggc tacaactcga
     1201 taggctactt cgcgcccgac atgcgctact cggccacggg aagcgtcagc gagttcaaga
     1261 cgatggtcaa gaggctgcac tcggcgggac tcgaggtgat tctagacgtc gtctacaacc
     1321 acaccgcaga gggcaaccat ctgggaccga cgctctcttt tcgcggcata gacaacaccg
     1381 cctactacag gctcgtcccc gacgacccgc gctactacat ggacttcaca gggacaggca
     1441 acacgctcaa catgatgcac ccgcgcgtcc tgcagctcat catggactct ttaaggtact
     1501 ggatccttga gatgcacgtc gacggcttcc gcttcgacct cgcgagcgcg ctggcccgcc
     1561 agctctatga agtggaccag ctctccagct tcttcaccat aatccatcag gacccggtaa
     1621 tctcccaggc taagctgatc gccgagccgt gggacgtggg tgagggcggg taccaggtcg
     1681 gcaactttcc gattggatgg gctgagtgga acggcaagta ccgcgacgtc gtgcgtgcct
     1741 actggaaggg tgagggcggg ctcgtggacg agctggccta ccggctcaca ggctcaagcg
     1801 acctctacga gcatggcgga cgcaggccct acgcgtccat caatttcgtc actgcccacg
     1861 acggcttcac tctgcgggac ctcgtctcct acgaggagaa acacaacgag gccaacggcg
     1921 aggacaacgg cgacggcgag aaccacaacc tgagccacaa ctacggcgtc gaagggccga
     1981 ccgacgacct ggagatccgg cgtatcaggg ccaggcagag acgcaatctc atggcgacgc
     2041 ttttgctctc gcagggggtg cccatgatcc tctccggcga cgagatgggc cgcacccaag
     2101 aaggcaacaa taacgcctac tgccaggaca acgagataag ctggctggac tgggaactct
     2161 ccatcctcga caggaacttc ctggccttcg tgcggcgcat gatccggctg cgcaaagaac
     2221 accccgtctt ccggcgccgc cgcttcttcc agggccggga gctgaggggc gctggggcga
     2281 aggacatcat ctggctcacc cccgctggcc gtgagatgac cgacgaggaa tggaacagct
     2341 ccttcgctcg atccctcggc ctccagatgt ccggtctcct cgaaggcgaa ttcgacgcgc
     2401 aggggcgtcc cgaggaggat gacgacttcc ttctgctttt gaacgccgac caagacgaga
     2461 tcatcttcga gctaccgccc ttccccgaag aggcccgctg ggaggtcttt atggacacgg
     2521 cctactccgc tggcctgaag accgacggct tcttcaaacc cggcggcacg tataccttga
     2581 agcctcgctc catggtcgtg ctcgtgaacg cccgcaggcg cgagaccgtc gaggaggacg
     2641 cagattgagg cgccactata tgcccttcgg cgcaaaggta ctggagcatg gagagacccg
     2701 cttcgccctt tgggcaccgg ccgccgaaac ggtagatctc atcctcgaag atggagtcct
     2761 cctgatgcac cgtgaggagg acgggacctt cgccctcacc acagaatccg gggctggcgc
     2821 acgctaccgc taccgggtag acggtgggca ggaggtgcca gatccggcct cccgctacca
     2881 gccggatgac gtacatggcg caagccgggt cgtggacccc gcggccttcg tctggaggga
     2941 ggagcactgg aagggacgtc cgtgggagga ggtcgtcctc tacgagctac acgtcggctc
     3001 ctttactccc gaggggacgt tcgccgggat caaggagaag ctcgactatc tcgtggacct
     3061 cggtgtgaca gcgatagagt tgatgccgct ctcggagttc cccggacggc gaaactgggg
     3121 ctacgatggc gtgttgccat tcgcccctga gagcgcatac ggggccccgg aggatctgaa
     3181 agatctcgta cagaccgcgc atacgaaggg cctcatggtc tttctcgatg tcgtctacaa
     3241 ccacttcggc cccgagggga actacctctc tctttatgct ccgcagttct tcacggaccg
     3301 ctaccagacc ccgtgggggg ctgcgatcaa cttcgacggc gagggcagca aacacgtgcg
     3361 tgagttcttt atccacaacg ccctgtactg gctggaagag tacggcatcg acggcctcag
     3421 actcgatgca gtgcacgcta tcaacgacgc ttctgagaag cacttcctag tcgagctcgc
     3481 cgagcgcgtg cgccagggtc caggacgcga ccgccacgtg cacctcgtac tggagaacga
     3541 ggcgaacgac gcctcctatc tgcgcgggca ctacgacgcc cagtggaacg atgatctcca
     3601 tcacgccctt cacgtcatcc tgaccggtga gagcgccggt tactacgctg attacgccga
     3661 cgcgccgatg cggcgtttgg ggaggtccct gggcgaggga tacgcgtacc agggggagcc
     3721 ctcgcgtcac ggcaagaagc gcggagagcc gagtgcggac cttccgtcag gtgccttcgt
     3781 atcgttcgtt cagaaccacg atcaggtcgg caaccgggct ttcggggaga gaatcgggca
     3841 actcgcgcct cccgaggcgg tgcgcgccgc ggcagagatc tacctgctag caccccagat
     3901 accgatgctc tttatggggg aggagtgggc ttccaagacc cccttcccgt tcttctgcga
     3961 cttcgcgccg gatctcgcgc tgctcgtcgc ccggggtcgc cgcgaggagt tttcggagtt
     4021 cttcgacccc gaggatgatg gtcgaatccc cgatcctggt gcggaagaga cgtttcgcag
     4081 ggccgtactg gactgggcga gtctggacga gccggagcat tccggacgtt tgaagctgta
     4141 tggcgagttg ctcgcactca ggcgagagaa gatcgtgccg cggctggtcg gcatacccgg
     4201 agacgtggcc gcgaaccaga tcgtagacga caggggcctg agcgtgcagt ggacactggc
     4261 tgacggctcc acgctggcac tcctcgcgaa cctgggaccg gagccgctcg atgaccccga
     4321 gtggccgccg ggcgaacggc tatatgccag cgaggatgtg accgggcaca gacaggaact
     4381 tcctggatgg tcggtgttgt ggcacctggc cgaatgagga ttccccgggc cacctacagg
     4441 ctacagctca gcgaggattt caccttttcg gatgcggcag agttggtgcc ttacctgtcg
     4501 gagctggggg ttagcgacct gtatgcctcg ccttatctga aagctcgacc cggcagtaca
     4561 cacggttacg acgtcgtgga ccccggctcc ctgagccccg aactcggggg cgagaaggag
     4621 tacggccggc tggtagagca cctcgaagaa tacgagatgg gccagctcct agatatcgta
     4681 ccgaaccata tgggcgtcgg gagcgacaac gcctggtggt ccgacatgct ggagaatgga
     4741 ccagcctcgg cctacgccgc cttcttcgat atagactggt gccccgcaga taggaaggag
     4801 ctgtgcggta aggtgctgct cccgatcctg ggcgaccact accgcgccgc cctcgaacgc
     4861 ggcgagatcc ggctggcctt cgacgccgga gagggttcct tctcagtcaa ctactacgaa
     4921 caccgctacc cgatcgaccc gcagacctac ccgatgatcc tcgaccggat atctttaccc
     4981 gaggacgacg agcaccgcca ggagatagat ggtctgatag tcgcttttgg cgaccttcca
     5041 ggtagggacg ccacaggtga ggggattgta tccgggcgat cgcagaacgc tgcaaacctc
     5101 aaagcggacc tcgcggcgtt gtgcggaaag tcgcccggag tattgggtgc tgtcgagaag
     5161 cacattcgcg aggtgaacga ggcgccagca gacccgaggt tcaagcgttt gcatcgtctc
     5221 cttgaggcgc aggcgtacag gcttgcgtac tggcgggtgg gaagcgatga gatcaactac
     5281 agacgtttct tcgccgtcaa tga
//
LOCUS       DATMJA010000247         4210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_448204, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000247 DATMJA010000000
VERSION     DATMJA010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448204"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VNT12_05925"
     CDS             complement(<1..1107)
                     /locus_tag="VNT12_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HWI45690.1"
                     /translation="MRTFFRFSERGTNLRTEVVAGLTTFMTMAYIVFVNPAILGTKGT
                     GLSVPGVFFATCVAAAVACIAMGLVANFPLALASGLGLNAIVAFTLILGQGLSWQQAM
                     ALIVVEGLLVTALVLTNLREAVLNAIPLSLKFAIAVGIGLFIAFIGLKNAGLVVPNTE
                     TYVALGDLTQGPVLLAAFGLIVTLGLVARGLRGGIIIGIFLTGIVGMIFGVVPTPKGI
                     VDFSFDTSTVGGGVLAVGQVLQLSLLPVIFALFMTDFFDTMGTMFAVAQEGKLLDDEG
                     RVPQARRILLVDSLAAVLGGSMGASSVTSYIESGAGVAEGGRTGMTSVVVGLLFLLAL
                     PFSPLISVFGGSLPIPDPTGGNDPLFVSPVTAPAL"
     gene            complement(1260..1934)
                     /locus_tag="VNT12_05930"
     CDS             complement(1260..1934)
                     /locus_tag="VNT12_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45691.1"
                     /translation="MDTRDKRPTSGGIEGRHYEERGLGPWIRRNPWLLMRLFTVVVFS
                     FAASTVAANFSYGLEGEPTVLRIDQVNSGRLPAGTEIGDYVQVSGTPDVGENLTRVGV
                     PESGIAVVSRYETAYFYFRLQETGDNLLIQTTQGLPKDLQSSGERVWRGKLETVGTVI
                     FHDTTQRGLENAGLPRDESIPVVNTGETPEYLRKIFPAYSAIIGLWLLSIAWLVWKRN
                     KPLLGL"
     gene            2034..2753
                     /locus_tag="VNT12_05935"
     CDS             2034..2753
                     /locus_tag="VNT12_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45692.1"
                     /translation="MQGLDDVCCWTAVSAVQSGLDPSLEVRVLVEGPEVQEQDSWYTV
                     VGVIDDGTGLNQAVTEVRELGVDRDDLTVVLKRVDSSESEPFPDGTRYIVVPADRRGL
                     EVPLGFAIAFIVFGIFFALTTPAIGIPTLLVFVSLAIILFAASLTRVGVNPILMEMEA
                     PQEEADAWNDQFEFGKVLVFASTRERRLLRPIREAMQRGGAIYYIVDRRLEPRAVHQA
                     TLHRTGGRQAGESVLERSGEA"
     gene            2767..4104
                     /locus_tag="VNT12_05940"
     CDS             2767..4104
                     /locus_tag="VNT12_05940"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWI45693.1"
                     /translation="MGVATTSSLHLLAQLNLSDAFSAVGGVRFITGLTMLVHMFWAEL
                     FVGFALAAPVLEAWGARTGSPRMDHLAHSMARFNVLTFSTGATFAVLFLVLLVGLYPP
                     VTASLFTHFFYAIAIAMLSMVLTLWGMYAYYYKWDRYSVLNKKRHIALGFAMGLFIWI
                     WMVIMTGIDSYMVTGGPGVKPLSGSTIESFSTAVQGIFNPMFTELVLHRTFANLSWPA
                     FAVAAWASFMYVRSKTQEDRAFYDWSSSVGLTWGVGFLMLQPLVGFTIVYALKFSRPQ
                     NPVSGADAGGAYERLTSGATSNLLLINLILVVVLIVLSNVAMYFGAERHPDQAGRVPI
                     RFFGLVAAVAGLYAVSPLAEFPFLYMRYIALLVMVLATFGAFVTYLRGRLRFRYGSPG
                     RRYRIVLMATGVVAAVIALNMGFMKSNSRVPYTVYNQPGYTVQPSPPPTGFGR"
BASE COUNT          758 a         1284 c         1346 g          822 t
ORIGIN      
        1 gagggcgggg gcggtcacgg gcgagacaaa cagagggtcg ttacctcccg ttgggtccgg
       61 tatcgggagg gagccgccga agaccgagat caagggcgag aacggcaggg cgagcagaaa
      121 gaggagcccg acgaccacgc tggtcatgcc ggtgcgtcct ccttcggcga cccctgcccc
      181 gctctctata tagctggtca ccgagctggc gcccatgctg ccgccgagga ccgccgccag
      241 cgagtcgacc agcagaatgc gcctggcctg cggcaccctg ccctcatcat cgaggagctt
      301 gccttcctgg gccacggcga acatagttcc catggtgtcg aagaaatctg tcatgaagag
      361 ggcgaagatc acgggcaaca gcgagagctg caagacttgc cccacggcga gcaccccgcc
      421 ccctacggtc gaggtgtcga agctgaagtc cactatcccc ttgggcgtag gcacgacccc
      481 gaagatcata cccacgatgc ccgttaggaa gatcccgata atgataccgc ctctcaggcc
      541 ccgcgcgacg agccctagcg tgactatgag tccgaatgcc gcgagcagca cggggccctg
      601 cgtgaggtcg cctagggcca cataggtctc agtgttgggc acgacgagac cggcgttctt
      661 caggccgatg aacgcgataa acaacccgat gccgacggcg atcgcgaact tgagcgagag
      721 ggggatggcg ttgagcaccg cctcgcgcag gttcgtcagc acgagcgcgg tgacgagcag
      781 gccttcgacc acgattaagg ccatcgcttg ctgccagctg agcccctggc ccaggatgag
      841 ggtgaaggct actatggcgt tcaggccgag ccccgaggct agcgccagcg ggaagttcgc
      901 cacgaggccc atcgctatgc acgccaccgc ggccgccacg cacgtggcga agaacacgcc
      961 gggtacggag aggccagtac ctttggtgcc caatattgcc gggttgacga agactatgta
     1021 agccatcgtc atgaaggtgg taagcccggc caccacctcc gtcctgaggt tcgtcccccg
     1081 ctcggaaaac ctgaagaagg tgcgcaaagt ctcgatctcc cctttgccgt tctatgtgcg
     1141 tgaaaaatat agcatttcag caagtcagcc aggacccgcg caggctgcaa gctgaagtgc
     1201 tttgaggcgc gaagcgccgt gctgacaagc gaagccgaag gtcggagcgt gctgaccggc
     1261 tacaaaccga gaagcggttt gttcctcttc cagaccagcc aggctatgga gaggagccag
     1321 aggccgataa tggccgagta ggccgggaag atcttgcgca ggtactcggg tgtctcgccc
     1381 gtgttgacga ccggtatgct ctcgtcgcgg ggaaggccgg cgttctcgag tcctctctgg
     1441 gttgtgtcgt ggaagatgac ggtccctacg gtctctagct tgcccctcca gacgcgctcg
     1501 cccgaactct gaaggtcttt gggcaggccc tgcgtggtct ggatgagaag gttgtcgccg
     1561 gtctcttgca ggcggaagta gaagtaggcg gtctcgtagc gggagacgac tgctattccg
     1621 gattcaggga cgcctacccg cgtcaggttc tccccgacat caggcgtgcc gctcacctgg
     1681 acgtagtctc ctatctctgt gcctgcgggc agtcttcctg agttcacctg atctatgcgc
     1741 agaacggtcg gctcgccttc caggccgtag gagaagttgg ctgcgaccgt cgaggccgca
     1801 aaggagaaaa cgacaaccgt gaacagccgc atgagcagcc acgggttgcg cctgatccag
     1861 ggccctagtc cccgctcttc gtagtgccgg ccctctattc cgcccgaagt tggccttttg
     1921 tccctggtgt ccacggacct agtctacccg agtgtgcggc aaggtttgga gtacggcttc
     1981 acatcctgcg gattgtgaaa gagattcccg cggggcgtgg ggaacggtct agtatgcagg
     2041 ggctcgacga tgtatgctgt tggacggctg tcagcgccgt gcagagcggt ctcgatccct
     2101 ctttggaggt cagggttttg gtggaagggc cggaggtaca ggagcaggac tcctggtaca
     2161 ccgtggtggg ggtaatagac gacggtacag ggctcaacca ggccgtcacg gaggtcaggg
     2221 aactcggcgt ggaccgcgat gacctcacag tggtcctcaa gcgcgtggat tccagcgagt
     2281 ctgagccgtt ccccgacggc acgcgataca tcgtggtccc tgccgacaga cgcgggctcg
     2341 aggtgcccct tggcttcgcc atcgctttca tagtcttcgg catcttcttc gctctaacaa
     2401 cgccggcgat cggaataccc accctcttgg tcttcgtctc gctggcgatc atactcttcg
     2461 ccgcctcgct cacgagggta ggggtcaacc cgatactgat ggagatggag gcaccccagg
     2521 aggaggcgga cgcttggaac gaccagttcg agttcggtaa ggtactcgtc ttcgcctcta
     2581 cgcgcgagcg ccggctgcta cgtcccataa gggaggccat gcagcgcggc ggcgcgattt
     2641 actacatagt ggaccggcgg ctggagcccc gggccgtcca ccaggcaacc ctgcaccgaa
     2701 ccggcggacg ccaggcgggg gagtcggtct tggaaagatc cggggaagcc tagataggag
     2761 tttagcgtgg gtgtagcaac cacttcgagc ctgcacctcc tggcccagtt gaacctttcg
     2821 gacgccttct cggccgtggg cggcgtccgc ttcattacgg gcctcacgat gctggtgcac
     2881 atgttctggg ctgaactatt cgtcgggttc gccctcgcgg cgccggtgtt ggaggcctgg
     2941 ggggcgcgca cgggtagccc gcggatggac catctcgccc actccatggc ccgcttcaac
     3001 gtgctcacgt tctcgacggg tgcgacgttc gcggtgctgt tcctcgttct gctcgtcggc
     3061 ctctatcctc ccgttactgc ttcactcttc acgcacttct tctacgcgat cgccatcgcc
     3121 atgctctcta tggtcctcac gctgtggggg atgtacgcct actattacaa gtgggaccgt
     3181 tacagtgtgc tgaacaagaa gcggcacatc gccctcgggt tcgcgatggg cttgtttatc
     3241 tggatctgga tggtcatcat gaccggcata gactcgtaca tggtgacggg cggacctggc
     3301 gtcaagccgc tctcaggctc gaccatcgag agcttcagca cggcggtgca gggtatcttc
     3361 aacccgatgt ttacggagct agtcctccac aggaccttcg ccaacctctc ctggcctgcc
     3421 ttcgccgtcg ccgcatgggc ttcgttcatg tacgtccgct cgaagacaca ggaggacagg
     3481 gccttctacg actggagcag ttccgtcggc cttacctggg gcgtggggtt cttgatgcta
     3541 cagccgctcg taggcttcac aatagtctac gcgctcaaat tctcccggcc ccagaatccc
     3601 gtgtcgggcg cggacgcagg gggagcttac gagcggctca cctcgggggc cacctcgaac
     3661 ctgctcctca taaacctcat cctggtcgtc gtgttgatcg tgctctccaa cgtggccatg
     3721 tacttcgggg cggagaggca tcccgaccag gccggccggg tgcctatccg gttcttcggc
     3781 ctcgtggccg cggtggccgg cctttacgcg gtctcgccgc tggccgagtt cccattcctg
     3841 tacatgcgct acatagcgct gctggttatg gtcctcgcca cgttcggggc gttcgtaacc
     3901 tatctgcgcg gcaggctgcg tttcaggtat ggcagcccgg ggaggaggta ccggatcgtg
     3961 ctgatggcta caggggtcgt tgcggccgtg atcgcgctca acatggggtt catgaaatcc
     4021 aattcgaggg tgccctacac ggtatataat cagcctggtt acacggtgca accgagtcct
     4081 ccgccgaccg ggttcggcag gtgaggctcg ggcggcccta gaagggacga agcgtggcgg
     4141 agctacagaa agatcccatg acccgcagcg acttcctcgg cttcggcgtg atgggagcga
     4201 tagtcggggc
//
LOCUS       DATMJA010000248         4134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_448484, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000248 DATMJA010000000
VERSION     DATMJA010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448484"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /gene="moaA"
                     /locus_tag="VNT12_05945"
     CDS             complement(<1..816)
                     /gene="moaA"
                     /locus_tag="VNT12_05945"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWI45694.1"
                     /translation="MTQLTDSYNRRMDYLRISVTDRCNFRCQYCMPEDIQFQDKSHIL
                     TLEEMLTFAEACLELGVTKVRVTGGEPLVRRGVVMFIGWLKDLGFEDVTMTTNGFLLE
                     DNLDGLVEAGLDRVNISLDTLQPEKFAFITRRNHFEKVWQAVMAALETPLSPVKINAV
                     AMRGVNDDEIADMARLTLRYPMHVRFIELMPLNGDTDGSRFRSLFIPGREILDRATEE
                     LGDLEPIEKEDPAAPADEFRFDGAPGTFGVITPVSEPFCARCSRLRLTADGKIH"
     gene            complement(1184..2398)
                     /locus_tag="VNT12_05950"
     CDS             complement(1184..2398)
                     /locus_tag="VNT12_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWI45695.1"
                     /translation="MEEIEHILRNFGLILTAGLVSQLIATVTRLPQMVVLVAAGALIG
                     PSVLGLVSNPLSGVGAQLLFNIGVALILFHGGTGISLRLISGTALGLGLLVLPGVLLT
                     AIIVALVVSPVFGVTFPVALLVGAVLAATDPAILIPLFDRLKLRPKVAQTVVAESAFN
                     DVTGTVLTLTLVGIVEAGGFTFSGPALEFTQELALGSLIGVVAGAILCYAISSTARAG
                     IWDESPGVAILAVVALAYFSTESLGGSAYLATFVMGLMVGNMEMLGLGHHDEHAMLLE
                     GFLGQVAEIATLLVFVTLGLNLPFEALGNYFFGGLLVMFVFIFVARPITVLACLLPDR
                     RGRWTGREIAFISWCRETGVIPAAVASLLLARGVEGAEIAVSMVALAVCVTLLLQATT
                     AGYVARRLGLLD"
     gene            complement(2402..3949)
                     /locus_tag="VNT12_05955"
     CDS             complement(2402..3949)
                     /locus_tag="VNT12_05955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWI45696.1"
                     /translation="MRIIMTHGYMLSGTGSNIYVQSLCRALVGEGHDVHLLCQEPDPL
                     AYDFVSKSAKVDARGVEDLGERETTYPGLCVVYQPEIGDLLPVYVYDDYPGWRVKTFL
                     DLTQEELDYYLRRNVEALKTVLGVSGVEAVITNHSVPGPLIARRAMEGNNLPYVSIVH
                     GSCLQYVSRKSEMYMGLTREGLEDAREILALSSHSAGTIAEDFPDLADRTHSLPGGVD
                     TELFHPDAFDNGVLDHLEGGPGRGQAQRTSLREALEMSGHAGELAGALRAVAASYDAR
                     SHDRDAGERLRHFLDREGPLLVYVGKLIHSKGVHSLISAFSRVRKETGARLLVIGFGT
                     FREGLEALTCSLSAGDETTVERLAEMGRLLEGGPAGPLEHFELAEEILVDAEGMQDDV
                     LFVGPLYHGDLTKLLPAADAAAVPSIFPETFGLVAAEFAASGVVPFVSDHSGLREAGE
                     IVGRGLPFDLRVSMDGFEDNFAKALSDYPMLPEEERRRCEEVVRRNCVKHLSWGTLAE
                     KLVALVG"
BASE COUNT          806 a         1297 c         1295 g          736 t
ORIGIN      
        1 atggatcttg ccgtccgccg tcaagcgtag cctgctgcaa cgggcgcaga agggttccga
       61 gaccggcgtt atgacaccga aggtgccagg ggcgccatcg aagcggaact cgtctgcggg
      121 ggctgcgggg tcttcctttt cgatgggttc gaggtccccc agctcctccg tggcgcggtc
      181 caggatctca cgcccgggga tgaagagcga acggaaacgc gagccgtcgg tgtcgccgtt
      241 taggggcatg agctctatga acctgacgtg catgggatac ctgagcgtaa gcctcgccat
      301 gtctgcgatc tcatcgtcgt tcacgccgcg catcgcgacg gcgttgatct tgaccggcga
      361 caagggcgtc tcgagcgccg ccatcaccgc ttgccagact ttctcgaagt ggttgcgccg
      421 cgttataaag gcgaacttct cgggctgcag ggtgtcgagg gagatgttga cacggtcgag
      481 gccggcttcg acgaggccat ccaggttatc ctcgagcaga aacccattgg tcgtcatggt
      541 tacgtcctcg aagcccaggt ccttgagcca cccgataaac atgacgactc cgcgcctgac
      601 gagcggctcg ccgcccgtta cccggacctt ggtcacgcct agctccaggc aggcttcggc
      661 gaaggtgagc atctcctcta gggtcaggat gtggctcttg tcctggaact ggatatcctc
      721 cggcatgcag tactggcagc ggaagttgca gcggtccgtg accgagatcc tgagataatc
      781 catcctgcgg ttgtagctgt cggtgagctg tgtcaaagca tcctttcggg cctgtgccta
      841 tcactagctc tcattggatt gtatcttctt tcagagacga gagtagcagc cgaatcgcct
      901 accccgagat gcgcgaccct acagtacgtt cggcaagatc cttatcccga agaagagata
      961 gcgataggtt gacaaggcgc gaagcgcctt gtcagcaagt cagcacgtcg cctgttccag
     1021 gcgacttgtc agcacttcag ctttttctcg gataggcccg tccaagctcg tgaactggaa
     1081 cgcctcagcg ggcgctactt cttacggacg gctgccgcta tctccacact cttgctgaca
     1141 tgctgaagtg ctgacgagcg aatgccgcaa ggccggagcg tgcttaatct agcaagccga
     1201 gacggcgggc gacgtagcct gctgtcgttg cttgcaggag aagagtgacg cagacggcaa
     1261 gggcgaccat ggagacggcg atctcagcgc cctcgacccc gcgggcgagg agcaggctcg
     1321 cgacggcggc ggggattaca cccgtctccc tacaccagct gataaaggcg atctctctgc
     1381 ccgtccatct tcctcgccgg tcaggcagta gacacgccag aacggttatg ggacgggcca
     1441 cgaagatgaa cacgaacatc accagtagcc cgccgaagaa gtagttgccg agcgcctcga
     1501 agggcaggtt gagtccgagg gtgacgaaaa cgagcagggt ggctatctca gcaacctgtc
     1561 cgaggaatcc ttcgaggagc atggcgtgct cgtcgtggtg accgagaccc agcatctcca
     1621 tgttcccgac catcagcccc atgacgaagg ttgcgaggta ggccgacccg ccgagagact
     1681 cggtcgagaa gtaagcgagc gccacgacgg cgaggatggc cacccccggc gactcgtccc
     1741 agatacctgc ccgcgccgtc gaggagatgg cgtagcagag gatcgctccg gcgacgacgc
     1801 cgatgaggga cccgagcgca agctcctggg tgaactccag agccggaccc gagaacgtga
     1861 acccgccggc ctccacaatg ccgacgagcg tcagggtcag taccgtgccc gtaacatcgt
     1921 tgaaagccga ctcggcgacg accgtctggg ctactttggg gcgtagcttc aggcggtcga
     1981 acagggggat gaggatggcg ggatctgtcg cggccaggac cgctccaacg agcagggcga
     2041 cggggaacgt tacgccgaac acaggtgaga ctaccagcgc gacgatgatg gctgtcagga
     2101 gtacgcccgg caagacgagc aagccgaggc ctaaggcagt acccgagatc aaacgtagcg
     2161 agatacccgt gccgccgtgg aacaggatga gggccacgcc tatgttgaac aggagttggg
     2221 cgccaacccc gctgagaggg ttggagacga gcccgagcac tgaaggcccg atcagggcgc
     2281 ccgccgcgac cagcactacc atctggggga gccttgtaac cgtcgctatg agctgcgaga
     2341 ccagcccagc agtcagtatg aggccgaagt tgcggaggat atgctcaatc tcctccaccc
     2401 tctaaccgac cagggctacg agcttctccg ccagcgtccc ccaactcaag tgcttcacgc
     2461 aattgcggcg cacgacctcc tcgcaccgcc gcctctcctc ctcaggcagc atcgggtagt
     2521 cgcttagcgc ctttgcgaaa ttatcctcga agccatccat gctgacgcgg aggtcgaagg
     2581 gcagaccacg gcccacgatc tcgccggcct ccctgaggcc ggagtggtca gacacgaaag
     2641 ggactacgcc gctcgcggcg aactccgcgg ccacgagccc gaaggtctcg ggaaagatgg
     2701 aggggactgc cgcagcgtcc gcggcgggaa gcaacttcgt caggtcgccg tggtagagcg
     2761 gcccgacgaa cagcacgtcg tcttgcatac cttcggcgtc gactagtatc tcctcggcga
     2821 gctcgaagtg ctccagagga ccagccggtc ctccttcgag taacctgccc atctcggcca
     2881 agcgctccac ggtcgtctcg tcgccggcgc tgagggagca ggtcagggcc tcgagcccct
     2941 cgcggaaggt gccgaagccg ataactagca agcgggctcc cgtctccttg cgaaccctgg
     3001 agaaggctga gatcaggctg tggacaccct tggagtggat cagctttccg acgtagacca
     3061 ggagcggacc ctcgcgatcg agaaaatgcc gcagccgctc accggcgtcc ctgtcgtgcg
     3121 agcgtgcatc gtaggaggcc gcgaccgcgc gcagtgcgcc ggccagctct cccgcgtgcc
     3181 ccgacatctc cagtgcttct cgtaaagacg tgcgttgcgc ctggccgcgg cccggtccac
     3241 cttcgagatg gtcgaggacc ccgttgtcga acgcgtcagg gtggaagagc tcggtgtcga
     3301 ctccgcctgg gagggagtgg gttcggtctg ccaggtcggg gaagtcctcg gctatcgtgc
     3361 ccgcgctgtg cgaggagagg gccaggatct cacgtgcatc ctccaggccc tcacgggtga
     3421 gacccatgta catctcgctc ttgcgcgaaa cgtactggag gcaacttccg tgtacgatgc
     3481 tcacgtaagg aaggttattc ccttccatgg cgcggcgtgc gatcagcggc ccaggcacag
     3541 agtggttggt gatcactgct tccacgccag acaccccgag cacggtcttc agggcctcga
     3601 cattgcgcct gaggtagtag tccagttcct cctgtgtcag gtccagaaag gtcttcacgc
     3661 gccatcccgg gtaatcgtcg tagacgtaga ctggtaggag gtccccgatc tcaggctggt
     3721 agacgacgca gagccccggg tatgtcgtct cccgctctcc cagatcctcg acccctcgtg
     3781 catctacctt cgcgctctta ctgacgaagt cataggcgag cgggtccggc tcctggcaga
     3841 gcagatgcac gtcgtgcccc tcgccgacca gagcgcgaca caggctctgg acgtagatgt
     3901 tactccccgt gccggagagc atgtacccgt gggtcatgat gatcctcatg gcgctccttt
     3961 tcgcccatgt cgagttccta tctccatgct cgcatagcgg aaggtatcac agagtccggc
     4021 tccagacccg caacgatggg ccgggctcca tcggcgtctg tcggacgtcc atcgtgccag
     4081 cctgcagtta acggccacag cctgcacgcg ccaagagcct ccacaggctg tagt
//
LOCUS       DATMJA010000249         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_567012, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000249 DATMJA010000000
VERSION     DATMJA010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567012"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VNT12_05960"
     CDS             complement(<1..797)
                     /locus_tag="VNT12_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWI45697.1"
                     /translation="MEERYLSARRAAEELGVSLATLYAYVSRGMVRSEAVEGKGRNRR
                     YRAEDVRRLKERRERRRDPDGVVEGALHWGTPVLESGITLVDKGGLYYRGRDVVDLAE
                     ERSIEEVAALIWTGDEAMAPALFPPEMSGPSRRIQNVVDSVAGLTPVEVFQIVLPVAA
                     AEDPAAYDLRPGAVARTGARILRLMTDVASGASVPGFAGTLQRGWSPDDPGAADLLDS
                     ALVFCVDHELPVSTFAARCVASSVATPYAVVLAGLAALGGVKHGGEI"
     gene            937..2067
                     /locus_tag="VNT12_05965"
     CDS             937..2067
                     /locus_tag="VNT12_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HWI45698.1"
                     /translation="MGESSGTGFVAGLEDVVAAETRLSGVDGEAGELTIAGFPVEELA
                     GRASFEETVYLLWHDVLPDSGRLADFREELAGRRELPPATLDLLRAVAAEKVPAMDAL
                     RMAAGVVSPGTDDEEALALVARLPTIVAAYSRMLEGEEPVDPDPELGHAANYLYMLTG
                     EEPGAEFTRALDTYLTTVSDHSMNASTFAARVIVATRSDFVSAVVGAIGALKGPLHGG
                     APGPALDVVFEIGDASRAEAYLREKLRRGERLMGFGHRIYRVRDPRADVLAAAAERLY
                     TTDGDKDLYELALDVEKTALSLLAEHKPGRNLQTNVEFYTALLLHGLGLPTQVFTPTF
                     AAGRVAGWTAHCFEQRELDRLIRPQSAYVGSRDGSWVPLEER"
BASE COUNT          408 a          696 c          698 g          393 t
ORIGIN      
        1 tcgatctctc ccccgtgctt tacgcccccg agagccgcaa gcccggccag aacaacggca
       61 tagggtgtgg ccacggacga ggcgacacac ctggcggcga aggtcgagac gggtaactcg
      121 tggtccacac aaaagacgag cgcggagtcg agaaggtcgg cagcccctgg atcgtccgga
      181 ctccagccac gctggagcgt cccggcaaac cccgggaccg atgctccgct ggctacgtcc
      241 gtcatcaggc ggagtatcct ggcgcctgtg cgcgcgacgg cgccaggacg caggtcgtag
      301 gcggccgggt cctcggcggc cgcaacaggg agcacgatct ggaagacctc gacgggcgtt
      361 agtccggcaa cgctgtccac aacgttctgg atacgccggg agggtccgga catctcgggt
      421 gggaagagcg ccggggccat cgcctcgtcg cccgtccaga tcaaggccgc gacctcctcg
      481 atgctcctct cctcggcgag atccacgacg tcgcgccccc tgtaatacag cccgcctttg
      541 tccaccaggg tgatcccaga ctcgagcaca ggtgtgcccc agtgcaaggc accctcgaca
      601 accccatcag gatcccgcct gcgctccctg cgttccttta gcctccttac gtcttcagcc
      661 cggtaacgcc tgttgcgtcc cttcccctcg accgcctcgg aacgtaccat ccccctgctc
      721 acgtacgcat acaaagtcgc cagactaacc cccaactcct ccgcagcccg cctggcactg
      781 agataccgct cctccatcca accgcctcct tcgctattct cgctatccct acccgaaacc
      841 gtactatata ttgatttatc attcaatatt gatacacgtt tcatgaacga ttaagatagg
      901 cgacatacga tgatggatca ggtagaaagg actgccatgg gcgagagttc tgggacaggt
      961 ttcgttgctg ggttggagga tgtggtggcg gccgagaccc gcctgagcgg tgtggacgga
     1021 gaggctgggg agttgaccat cgcgggtttc ccggtcgagg agttggctgg gcgggcttcc
     1081 ttcgaggaga ccgtgtacct actctggcac gatgtcttgc ctgattccgg gcgactcgcg
     1141 gattttcggg aggagctcgc gggacggcgg gagctgccgc cggctacttt ggatctgctg
     1201 cgggccgtgg ctgcggagaa ggtgccggcg atggatgcgc tgcgcatggc ggccggcgtc
     1261 gtgagccccg gcacggacga tgaggaggct ctggcgctgg tggcgcgtct gcccaccatt
     1321 gtggcggcat actcgcgcat gctcgagggc gaagagcctg tggaccctga tcccgagctg
     1381 ggccatgcgg ccaactattt gtacatgctc actggcgaag agccaggcgc agagttcacg
     1441 cgggccctcg atacatacct tactactgta tccgaccaca gcatgaacgc ttccacgttc
     1501 gcagcgaggg tcattgttgc tacacgctcg gacttcgtct ccgccgtagt cggggcgatc
     1561 ggtgcgctca aggggccgtt gcacggcggc gcgccaggac ccgccctcga tgtggtgttc
     1621 gagatcgggg atgcgagcag ggccgaggcg tacttgcggg agaagctcag gcgcggcgag
     1681 cggctcatgg gtttcgggca caggatctac cgcgtacgcg acccgagggc agacgtactc
     1741 gccgccgctg ccgagcgcct gtacaccacc gacggagata aagatctcta cgagctggcg
     1801 ctcgacgtcg aaaagactgc gctctcattg ctcgcggagc acaagcccgg caggaacctg
     1861 cagaccaacg tcgagttcta cacggcgctc ctgcttcacg gactcgggtt gcccacgcag
     1921 gtcttcaccc ctaccttcgc agctgggagg gtggctgggt ggacggcgca ttgcttcgag
     1981 cagagggagc tcgaccgtct tatcaggcca cagtccgcgt acgtggggag tagggatgga
     2041 agctgggtgc cgctcgaaga acggtagtgt ggaaagacac gaaaaacgac ccccggagaa
     2101 atccgagggc cgtacccttc tgagcgttac gcaggcatcc cgtctttcag cacgtccgcc
     2161 tacactacat gggaggcgga taaagtaacc cagga
//
LOCUS       DATMJA010000250         3604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_448572, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000250 DATMJA010000000
VERSION     DATMJA010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448572"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..744
                     /locus_tag="VNT12_05970"
     CDS             127..744
                     /locus_tag="VNT12_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWI45699.1"
                     /translation="MSSCNSVAYIGDSTSEGMVLPNYLPDPGHRLGAQYARVGATEQY
                     FEISGARSIVETLSENQASGYAIARDLKAHGFQGCWVIALGSNDTANVYVGSMVGLEA
                     RIRRMMRVMGDQPVLWITVKSLLDTGPYSEKNMRHWNRTVVDACAKYPNMRVFDWASL
                     AEDGWYISDGTHYTSEGYRHRARLSADALAHAFPASGERSDCVVK"
     gene            829..1188
                     /locus_tag="VNT12_05975"
     CDS             829..1188
                     /locus_tag="VNT12_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45700.1"
                     /translation="MKVIADRIVTLGFGKYVRADNIVALVPIEEDRGSGRRTLVYVDG
                     LPEPLVAGRTEATILRDMVGAESGAAELLRELQLQIGQVRPLLKSSIRSEAGLDLDEL
                     SRRITDLTGEQASPKMF"
     gene            1185..1877
                     /locus_tag="VNT12_05980"
     CDS             1185..1877
                     /locus_tag="VNT12_05980"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWI45701.1"
                     /translation="MKAAFYDRDPRQVAIDLLGCVLTHDTPEGRTAGVIVETEAYRPE
                     DPACHAYKGPTMRNRNIFGPPGIAYVYLSYGTHKLLNVVCEPEGVGSAVLIRALRPSE
                     GEDLMERRRGRALDLCNGPGRLTQALGVGLSYDGQDLTSGSLIISDGGAPPEGIVETT
                     RIGITRGTDLPWRYLVDGDQHVSVPPKTVSGRRRRAESSRPGSGAKILYDAPVAVPAF
                     GPPWFPRLVFFW"
     gene            1956..3116
                     /gene="tyrS"
                     /locus_tag="VNT12_05985"
     CDS             1956..3116
                     /gene="tyrS"
                     /locus_tag="VNT12_05985"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWI45702.1"
                     /translation="MKASLEHRTIYRNAVDVIPREELDRRLAGGETLRVKLGIDPTAP
                     DIHLGFAVVLRELRAFQDLGHTAVLVIGDYTARVGDPSGRSKTRPILSPEQIEENTRT
                     YLEQAYVILDRGRTEVRHNSEWLAPLTMADLIMLTRATTLARILERDDFSKRYAANDQ
                     ITLTELLYPLMQAYDSVAIEADVELGGTDQLYNLLMGRQVMEYYGMRPQCVVTTPLLV
                     GTDGKIKMSKSVGNYIGVTDPPGEMFGKVMSIPDQLMDDYYSLLLDRPMPEGEPVEQK
                     RELARSLVSTFHGESAAADAEQLFDAVVRREVPDNAPVVQLPDDEEIWIVDLITRAGF
                     ASTNGEARRFIKGGAVRVGGRVVRDEGLSLSSRELEGQVLQVGKRRYARLAL"
     gene            3477..>3604
                     /locus_tag="VNT12_05990"
     rRNA            3477..>3604
                     /locus_tag="VNT12_05990"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          736 a         1052 c         1147 g          669 t
ORIGIN      
        1 gaagctacgt caccggagaa aacgtcgtcg gagactgcag cacgcacctc cactcagaca
       61 cgagagccag cgtcacaaaa accgaagtca ctagagccgg agtcgcgaaa ggcttcctcg
      121 cccgacatgt cctcctgcaa ctcggttgct tacatcggtg actctacgtc ggaaggcatg
      181 gtcttgccca actatttgcc cgatcccggc caccgcctgg gggcgcagta cgcgcgggtc
      241 ggcgctacgg agcaatactt cgagatctcg ggggccagat cgatagtcga aacgctatct
      301 gagaatcagg ccagcggcta cgcgatagcc cgtgatctga aggcccatgg cttccaggga
      361 tgctgggtga tcgcgctcgg cagcaacgac accgctaatg tctacgtggg ttcgatggtg
      421 gggctggagg ccaggatcag gcggatgatg cgggtgatgg gcgaccagcc ggtgttgtgg
      481 atcaccgtga agtcgctgct cgataccgga ccctactcgg agaaaaacat gcggcactgg
      541 aatcgcaccg ttgtagacgc ctgcgcaaag tacccgaaca tgcgggtctt cgactgggct
      601 tcgctggcgg aagacggttg gtatatctca gacgggaccc actatacttc ggagggctac
      661 aggcaccgcg cccgtttgtc tgcggacgcg ctggctcacg cgttccccgc ctcgggagag
      721 cgttcggatt gtgtcgtcaa gtagcctgtg gcggcctttc cggcgtgacg ggtcgcccgg
      781 cgctggcggg gatgttataa gggtctgtag gatggagatt gtgtcgagat gaaggttata
      841 gcggaccgta tcgtcaccct cgggttcgga aaatacgtaa gggccgacaa catcgtggcc
      901 ctcgtcccga tagaagagga ccgcggaagc ggacgccgca cgctcgtcta cgtcgacggt
      961 ctgcccgagc cactcgtcgc cggaaggacg gaggccacga tcctcaggga catggtcggc
     1021 gccgagagcg gggcggcaga gctactccgc gagttgcaac tccagatcgg gcaggtccgt
     1081 cccctgttga aatcttccat tcgctcggag gccgggctcg acctcgacga gctctcccgc
     1141 cgcataacag accttacggg ggagcaggca tctcccaaga tgttctgaag gccgcttttt
     1201 acgataggga tcccaggcag gtcgccatcg accttctcgg ctgcgtcctc acccacgaca
     1261 cacccgaggg acgtacggcg ggcgtgatcg tggagacaga agcgtaccgt cccgaggacc
     1321 cggcatgcca cgcctacaaa ggtccgacga tgcgcaaccg taacatcttc ggcccgcccg
     1381 gtatcgccta cgtctacctc tcctacggca cgcacaaact actcaacgta gtctgcgagc
     1441 ccgagggcgt cgggagcgcc gttttgatca gggcgctacg tccctccgag ggcgaagatc
     1501 tgatggagcg gcgtcggggc cgcgcgcttg acctttgcaa cggccctggc cgcctgaccc
     1561 aggcactcgg agtcggtttg tcttacgacg ggcaggatct cacctcaggt agcctgatta
     1621 tctcggacgg aggcgcgccg cccgagggta tagtcgagac caccaggatc ggcataacac
     1681 gcgggacaga ccttccgtgg cgttatctgg tcgacggaga ccaacacgtc tccgtgccac
     1741 cgaagactgt ctcggggcgg cgccggcgcg cagaatcctc gaggccaggc tccggcgcga
     1801 agatccttta cgatgctccg gtagccgtac cggctttcgg tccaccctgg ttcccccgac
     1861 tcgttttctt ctggtaggtg cgggccatcg agccggtata ctacactagg gtgacgcagg
     1921 taacattcgt attacgagaa ggttgcgatc tgttaatgaa ggcctcttta gagcaccgaa
     1981 cgatctacag gaacgctgta gatgtgatcc cacgggagga gctcgaccgc cgcctcgctg
     2041 gtggcgagac tttgcgggta aaactgggta tagaccccac ggcccctgac atacatctcg
     2101 gcttcgctgt ggtactgagg gagctccgtg ccttccagga cctggggcac accgctgtac
     2161 tcgtgatagg ggattatacg gcgcgggtcg gcgacccttc cgggcgttcg aagacgcgtc
     2221 cgatcctctc gcctgagcag atcgaggaga acacgcggac ctacctcgag caggcgtatg
     2281 taatcctcga ccgcggtcgg accgaggtcc gccataactc agagtggctc gcgccgctga
     2341 cgatggcaga tcttataatg ctgacaaggg cgacgacctt ggcccgcatc ctggagcgcg
     2401 acgacttcag caaacgctac gctgcgaacg accagatcac gttgaccgag ctcctctatc
     2461 cgctgatgca agcctacgat tcagtcgcga tagaagccga tgtcgagctc ggcggcacgg
     2521 atcagctcta taacctgctc atgggtaggc aggtgatgga gtactatggg atgaggcccc
     2581 agtgcgtagt cacaaccccg ctcctcgtcg gcaccgacgg caagatcaag atgagcaagt
     2641 ccgtcggcaa ctacataggc gtaacagacc cgccgggtga gatgttcggt aaggtcatga
     2701 gcatccccga ccaactgatg gacgattact actcccttct gctcgacagg ccgatgccgg
     2761 agggcgagcc cgtcgagcag aaacgcgagc tggcccgctc gctggttagt acttttcacg
     2821 gggagtctgc cgcagccgac gccgagcagc tctttgatgc cgtcgtgcgc cgcgaggtac
     2881 cagataatgc gcctgtggtc cagttgccag acgacgagga gatctggata gtagacctga
     2941 tcacacgcgc aggctttgcg agcacgaacg gcgaggcccg ccgctttata aagggcgggg
     3001 ccgtgagggt cggcgggagg gtcgtacgcg acgagggact ctcgctttcg agtagagagc
     3061 tcgaaggcca ggtgctccag gtgggcaagc gccgctacgc ccgtctcgca ctttgaaaaa
     3121 cagcgaaaaa cgagtttgac acctcggggg ctggtgctat acttctctag ctgatgacgg
     3181 ggaactactt gaaaatcgag aggggcgcat agggtagacc tcctgacaga gtgggagtgc
     3241 tctcttggcc cgccgaaagg tgggcgtttt agtcgccttt ccaaggtaga ctccgtcggc
     3301 tccgccgaga ggcaggagct tgaggctttg aaaactggat agcagtgaag attgggtaga
     3361 gaacccgtct aaccgccgag actgactctt tgcggtccag gagtgtaatt gtggaccgcc
     3421 gagcgggtag cgcccgctaa gacagtacca acaggcgctt cggcgccaac aggtatcgtt
     3481 cggagagttt gatcttggct caggacgaac gctgacggtg tgctttaggc atgcaagtcg
     3541 aacgagaaag cccttcgggg tgagtagagt ggcgaacggg tgagtaacac gtgggcaacc
     3601 tgcc
//
LOCUS       DATMJA010000251         3277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_153054, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000251 DATMJA010000000
VERSION     DATMJA010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153054"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1069
                     /locus_tag="VNT12_05995"
     CDS             200..1069
                     /locus_tag="VNT12_05995"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWI45703.1"
                     /translation="MQEIEGVYVANVTPFKDDGSLDVDVGAYLEHVSWLTEMGVRGIV
                     PLGTNGEGPSVMLGEKLRVLEALFGRGFPIQVIPAVMQNNLPETLEMLRILEDYPATA
                     VLVLPPYYFKPVEPEGMVRFYEPVLAATGHTVIVYHIPKYAVPVPDRVVKQLSIWGVK
                     DSGGEEGYAEAVLEGGKGVLLGTEDDLWRRLGTGAQGIISALANFIPEEVLDLYEKHR
                     EGDEAGGMALSERLQMARAITKEHASPAVLKKLAQARHGARMGTVRPPFVPVPADYDP
                     TPALQAVGQGAGR"
     gene            1066..1332
                     /locus_tag="VNT12_06000"
     CDS             1066..1332
                     /locus_tag="VNT12_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45704.1"
                     /translation="MKISDITTICTAPEGIRLVVVKVETDERTREVFPGCPEIRDGYM
                     WPSESPGLGVDIDEDLAAKYPFPEDPLNGGWPPVRRLDGTVIRP"
     gene            1349..2293
                     /locus_tag="VNT12_06005"
     CDS             1349..2293
                     /locus_tag="VNT12_06005"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWI45705.1"
                     /translation="MEMRGCYPILATPFLPDGEIDANSVVRLVRHLKTCGLPGFTMFG
                     LASEFYKLSDADRETLIEAAFDARALDQTAIVSVTAHSHEVAVKQARRAEEAGAGALM
                     LLPPFFLGSSEDDIRRHVLEVAEATSLPVMVQYAPAQTGVKMAAKSFLELNRRAPNVC
                     YVKVESLPSGPLISAIAEGSSGEIKCLMGYGGLQLVDGLRRGAVGVQPGSGVADYYPH
                     ILRAFEGGDLERAYALHADLLPLVNLLMQAIEPLNRLEKIVLEKRGIIAHDYCRAASF
                     EPDDLMLAELDLFVGRIAGRLHLSAPWPTVEEQRPFFE"
     gene            complement(2320..2541)
                     /locus_tag="VNT12_06010"
     CDS             complement(2320..2541)
                     /locus_tag="VNT12_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45706.1"
                     /translation="MNDWLSVELARERNARILAEAEERRLARLAPRRPVWTALRARVA
                     RKMFQLAVALEKEETWRAVWERLEAPRHP"
     gene            2646..>3277
                     /locus_tag="VNT12_06015"
     CDS             2646..>3277
                     /locus_tag="VNT12_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HWI45707.1"
                     /translation="MYGYRPTTRLLSMLELLQARGRMGGPELARRLEVGERTVRRYVA
                     MLQEMGVPVEAERGRYGAYTLRPGFKLPPMMFTDEEALALALGLLSARRLGLSGAAPA
                     VEGAQAKLERAMPEGLRERVRTFEEVVVPAAPARLPAGEIVITLSAAVRERKRVWMRY
                     RSGDAGETQRAVDPYAVVQGDAYWYTFGYCHLREGRRLFRLDRVLSVERL"
BASE COUNT          606 a         1019 c         1055 g          597 t
ORIGIN      
        1 tttctcgatg atgagagatc tctggggaga tgatacgaaa aattcgcaag aacaattgtg
       61 aagttggcat tgggaaaggg ccacctgatg gaccattcca aattcctgat tctcaattcc
      121 taattcaaaa gggatctacc cgattacgtg tgagccgcgt ggggaatggt aattccagga
      181 ctctacggga ggtgcgagca tgcaagagat agaaggcgtt tacgtggcta atgtgacgcc
      241 gttcaaggac gatggttccc tcgatgtgga cgttggggcg tacctggaac acgtctcctg
      301 gctgaccgag atgggcgtgc ggggcatcgt gcccttgggc accaacggcg agggaccatc
      361 ggtgatgctc ggtgagaagc tgcgggttct cgaggcgctg ttcgggcgcg ggttcccgat
      421 ccaggtcatc cccgccgtca tgcagaataa cctgcccgag accctggaga tgctgcgcat
      481 cctcgaggac tatcctgcta ccgctgtgct cgtcctgccg ccctactact tcaagcccgt
      541 cgagccggag ggaatggttc gcttctacga acccgtcctc gcggcgacgg gccatactgt
      601 catcgtctac cacatcccca agtacgccgt gcctgttccc gatcgggtcg tgaagcaact
      661 ctccatctgg ggcgtcaagg actcgggcgg agaggaaggc tacgccgagg ccgttctaga
      721 aggcggcaag ggtgtgctcc tcggtacgga ggatgatctc tggcggaggc taggtactgg
      781 ggcccagggt attatctccg ccctggccaa cttcatcccc gaagaggtgc tcgacctgta
      841 cgaaaagcat cgggaaggcg acgaggccgg aggcatggcc ctctccgaga ggctccagat
      901 ggcacgggcg ataaccaagg agcacgcctc tcccgcggtc ctcaagaagc ttgctcaggc
      961 acggcacggg gctcgaatgg gaaccgtcag gccgcctttc gtgcccgttc cggcggacta
     1021 cgatcccacc cccgcgctgc aagccgtagg acagggagcc ggcaggtgaa gatctcggac
     1081 ataacaacga tctgcacggc gccggagggc ataaggctcg tggtcgtcaa ggtcgagacc
     1141 gacgagcgca cccgcgaagt cttccccgga tgcccggaga tccgggacgg ctacatgtgg
     1201 ccttccgaat cgcccggact cggcgtagat atagacgaag atctcgcggc caagtaccct
     1261 ttccctgagg acccgctaaa cgggggctgg ccgcccgtca ggcgtctgga tgggacggtg
     1321 atccggccgt gaaagaacgt aattgaccgt ggaaatgcgc ggctgctacc cgatcctggc
     1381 cacgccgttc ctccccgacg gcgagataga tgcgaatagc gtcgtccgcc tcgtcaggca
     1441 cctgaagacc tgcgggcttc ctggtttcac gatgttcggg cttgcgagcg agttctacaa
     1501 gctctccgat gctgacagag agaccctgat cgaagccgcc ttcgatgcca gggccttaga
     1561 ccagaccgct atcgtctccg tgacggccca cagccacgag gtcgccgtaa agcaggccag
     1621 gcgggccgag gaggcaggcg ccggggcgct catgctcctc ccacccttct ttctcggatc
     1681 ttccgaggac gacatcagga gacacgtact cgaggttgca gaagccactt cgctcccggt
     1741 catggtccag tacgcccctg cccagacggg ggtgaagatg gccgccaaat ctttcctgga
     1801 actcaaccgg cgggctccga acgtatgcta cgtcaaggtc gagagcctcc cgtccgggcc
     1861 actgatctcc gccatcgccg aaggctcctc tggtgagatc aaatgcctca tgggttacgg
     1921 cggactgcaa ctcgtggatg gattgcggcg cggggctgtc ggcgttcagc cgggctcggg
     1981 cgtggccgac tactacccgc atatcctgag ggctttcgaa ggcggcgacc tcgaaagggc
     2041 ctacgccctg cacgccgacc tcctgccgct cgtcaacctg ctcatgcagg ccatagagcc
     2101 gctcaacagg ctggagaaga tcgtcctcga gaaacgcggc atcatcgccc acgactactg
     2161 ccgggccgcg agcttcgagc ccgacgacct gatgctcgcc gagctggatc tcttcgtcgg
     2221 acgcatcgct ggcaggttgc acctttccgc gccgtggccg accgtcgaag aacaacgacc
     2281 attcttcgaa tgaagatagg taggacctaa ccggtactct tacgggtgcc gcggcgcctc
     2341 caacctctcc cacacggccc gccaggtctc ttctttctcc agcgccaccg ctaactggaa
     2401 catcttcctc gccacccgcg cccgcagcgc ggtccacacc ggcctgcggg gggccagccg
     2461 cgcgagccgc cgctcctcgg cctcggccag aatccttgcg ttcctctccc gcgcgagctc
     2521 cacagataac caatcgttca cgacgtgtat ctcctctctc gcttgcctga tgagaatact
     2581 ggcgaatgag gtcataatcc ttcctgataa cctgtggaac ggcgacgttt ttcggaggta
     2641 acagggtgta cggctaccgc ccgacgacga ggttgctctc gatgctggag ttgctccagg
     2701 cgcgggggag gatggggggt cctgaactgg cccgcaggct cgaggtcggc gagaggaccg
     2761 tgcgccgata cgtggcgatg ctccaggaga tgggcgtacc cgtggaggcc gagaggggca
     2821 ggtatggcgc ctataccctg aggcctggct tcaagctgcc gccgatgatg ttcaccgacg
     2881 aagaggcgct cgcgctggcc ctggggctgc tctcggcccg caggctcggt ctgtcggggg
     2941 ccgcccccgc cgtcgaaggc gcccaggcga agctggagcg ggcgatgccg gagggtttgc
     3001 gcgagcgggt gcggaccttc gaagaggtcg tcgttcccgc ggccccggcg cgcctccccg
     3061 cgggtgagat cgtgattacc ttgagcgcgg ctgtccggga gcggaagcgg gtatggatga
     3121 gataccggtc aggtgacgcg ggggagactc agagggccgt ggacccctac gccgtcgtgc
     3181 agggggatgc ctactggtac accttcggtt actgccacct gcgggaagga cgacgcctgt
     3241 tcaggttgga tcgggtactc tcggtggaga ggctcga
//
LOCUS       DATMJA010000252        10415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_685775, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000252 DATMJA010000000
VERSION     DATMJA010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685775"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            606..2136
                     /locus_tag="VNT12_06020"
                     /pseudo
     CDS             606..2136
                     /locus_tag="VNT12_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2417..2677)
                     /locus_tag="VNT12_06025"
     CDS             complement(2417..2677)
                     /locus_tag="VNT12_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45708.1"
                     /translation="MESELSGNQHQLLRAFDELAGGSPTTDVTPSAAAQEAGFDLDSR
                     EFEATLGYLVDVGYLARAGDEGEETFRITVAGINEVQRRPQL"
     gene            complement(3006..4550)
                     /locus_tag="VNT12_06030"
     CDS             complement(3006..4550)
                     /locus_tag="VNT12_06030"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWI45709.1"
                     /translation="MNGAESLVETLAGAGVEVCFANPGTSEMHFVAALDQVGDMRCVL
                     GLFEGVATGAADGYARMADKPASTLLHLGPGLANGLANVHNARRANTPMVNIVGDHAT
                     YHKEYDAPLTSDVEGAARPFSDWVMTSADSRSVARDGADAVAAAMAPPGRVATLILPA
                     DASWNEADRPAPLPPVEGREPVAPEAISDVARALRSSEPAVLFLGGRALREPGLMLAG
                     RIAAKTGARLLAQTLNARVERGAGRVAIDFLPYPVDDALATLADARHLVLVGSEAPVA
                     FFAYPDKPSALSPPPCETHTLARPGEDIVGTLEALAEELDAPQNAPVQAQEKYPPPSG
                     EAITPETAAAAVGTLLPEGAIVVDEALSAARAFMSRTRGAAAHDYLQVTGGAIGIGLP
                     LATGAAVACPDRKVICLQADGSAMYTVQALWTQAREGLDLTTLLLNNRSYAILKQELT
                     NFGAGDAGRRALDMMELDRPALDWVGLARGMGVEAGRAKTMEELERQLAAGLSSEGPY
                     LIDLML"
     gene            complement(4827..5192)
                     /locus_tag="VNT12_06035"
     CDS             complement(4827..5192)
                     /locus_tag="VNT12_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWI45710.1"
                     /translation="MSADENKALVRRFVDEVQSRGNIDALDEICSPEFVNHSAPPGVP
                     SNCEGVKQLTAMFRQAFPDSYFTVEDMMAEGDKVATRRTFHGSHQGEFMGIPPAGGCL
                     WGSLTSCASPMGGWWNTGP"
     gene            5397..6566
                     /locus_tag="VNT12_06040"
     CDS             5397..6566
                     /locus_tag="VNT12_06040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWI45711.1"
                     /translation="MSVEENMALARRFLESQVKGDLEAMDEMMAPDFVGHTKLLPDQQ
                     PDREGQKWAYAQFSAATSNASIDFEDQIAAGDKVVTRFIVHATHDRGELMGVAPSGKE
                     LVYMPIGIHRIEGGKIAEQWGGGTGLSELRGLRLEQEERERERVEQELRVARRIQQAS
                     LPKVVPRLEGWQIFPYYWPAREVGGDFYDFFELVDDRVGVVVGDATGKGMPAALVAEA
                     TSNMLRAVAQALGSSSPSEILARVNETLLARIPPNMFATCFYAILEPKSGRLHYANAG
                     HNLPCCRHGERQTDELVARGMPLGLMPGMDYEEKEAVLAPGDCALFYTDGLIEAHNPQ
                     GEMYGTPRLRNLLSERPEGGTGLHATLMKELERFTGEGWEQEDDVTLLTLERSAA"
     gene            6884..7087
                     /locus_tag="VNT12_06045"
     CDS             6884..7087
                     /locus_tag="VNT12_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45712.1"
                     /translation="MPEPVNVKCPSCDENLAATDESDLVNVLQEHLYEEHALEMPRER
                     VRENVVAQLRDFDGYNARPITGA"
     gene            7205..7921
                     /locus_tag="VNT12_06050"
     CDS             7205..7921
                     /locus_tag="VNT12_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI45713.1"
                     /translation="MVTLVAVLAVAVAAGAVIQGSIGFGYALVAVPAMVLLLPWAVPV
                     TPLFLALPMTLFMSVREWRSIDLSGFALITAGRLLGTVAGVVLLVLAPKGYLSIVTGL
                     LILTVALGSFLKPTFEMNKRTRLAGGVSSGVVGTVAALGGTPLALVYQDRSGAELRST
                     LAISFVVGIVMSLVGLGLAGKVEGRHVVLALELLPGLLVGLWASEWVAKRLDERWLRP
                     TVLMFAAVAGVVIILLGPGG"
     gene            8154..9050
                     /locus_tag="VNT12_06055"
     CDS             8154..9050
                     /locus_tag="VNT12_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWI45714.1"
                     /translation="MRYGVSVPNFGVGVDARVIAELAREAEEAGWDGFFLWDHLLAFS
                     PGPVPVVDPWVALSAVALSTSRLRLGPMVTPLPRRRPGKLARETVSLDHLSDGRLILG
                     VGIGAMPYEYDYLGEEADTRVRGAMLDEGLEVLTGLWSGEPFGHRGEHYRVAGDPPEQ
                     DWRAVFYPPPLQRPRIPIWVGGTWPIKAPFRRAARWDGVFPMKVEGGRIVPMTPEDAQ
                     EVAHYIAAHRAGDGPFELVVAGETPGDDRKKAARIMSAFEEAGVTWWIESIDTWRFGW
                     TEGETWPSEEMRHRVRRGPPEA"
     gene            9642..9905
                     /locus_tag="VNT12_06060"
     CDS             9642..9905
                     /locus_tag="VNT12_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45715.1"
                     /translation="MRLYPWPDATVAGEVEAEELRKVAQAQWPKPWAAWGFDAADLGG
                     PGTATLAVLEGDAVAHVERTLGRQVEWRQTGVGLREAVGRALG"
     gene            complement(9937..10233)
                     /locus_tag="VNT12_06065"
     CDS             complement(9937..10233)
                     /locus_tag="VNT12_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45716.1"
                     /translation="MGDTSVCYRDAWESTHPEDPGHTFTPRNPLVTSGDNWPLELGPR
                     IDYIMVRCGHHGPTLDVSSCERIFDEPVDGVWASDHFGVVAELSAQTRSGRPLP"
BASE COUNT         1838 a         3185 c         3437 g         1955 t
ORIGIN      
        1 gtgcgtgaag ctgtaagggt ccaacaggtg ctgggagttg tccgaactcc aggggtcccc
       61 ggaccacagg agcaggtagt cacaggaaca ccaccacagg cgtccttggc tgcggagtac
      121 taacgcagtg ccagccagga ccacgacgat ggcgagccac ggcaaaatcc ttcgcatcct
      181 gcgctgaaga gtatgcgatc gtcattagtg gcgctagcgg ctgactgcag atctcagcgg
      241 tggttctctc ccaccgtgcg cgcgagaggc gacggcgggg tttctaccgc ctcaaggtgg
      301 gcccacgcaa gttgggacaa cgcttagctt gctgttccgt gccgttttga ttgacagagg
      361 gaagcccgcg agggtccaag tctcgctcta gtgatgccgt aaggtgggtt gagctaagta
      421 acgtcactat gacagccagg accgggtcat ccttaatagg agcctcggcc cttttcagtt
      481 attatcggca gtagtctatg gggggtgcgc cgacaactgc ttgggcggta tcctgccaaa
      541 gcaacatcag tgagccgaac gctgctgaag taggatcggc tagccgaaaa cctgggaggt
      601 tgaggatgca tctggtcacc gagcgtaatc gcaggtggtg ggtcgtgata gcgatggcct
      661 taaccaccct cctcatgacc atcgacttca acgggctcac ggtcgccctg cccaccatag
      721 gccgggatct cgacacctcc actaccggtc tgcagtggac catcaacgcc tacctgctgg
      781 cccttgcggc tccatcggtc gcggccggac gcctggctga catcttcggc cggcgcaagg
      841 tactgttgat cggcaccgtc gtgttcatcg cgggttctgc agcctcggga ttggcgcagg
      901 ccgactggtg gcttatagcc gcacgcgtcg tgcagggctt cggagcggcc gcgttattcg
      961 ccgcctccct ctctatcgtc agcaacgctt tccctcctga ggagcggagc aagggaatcg
     1021 gcgtgtgggc cggtataggc accgtggggc tggttcttct tcgtgaacat acccgtggct
     1081 gtcctcgcca tcatcctgac cctggtgacc gtgcgcgagt ccaaagacga gagcgcaagt
     1141 gcccacgtag acctccttgg attgctcacg atcaccttgg gtctcggagc gctggtgctg
     1201 gccatccagc agggtgacac cctgggatgg agctcggtgc tgatccttgg atccctggcc
     1261 gtggcgttgg tcctgctcgg actgttctgt ttcgccgaac tgcgcctgcg cgaaccgctg
     1321 atagatctgg gtctcttcgc caaccgtggt tacctcgggg ccaacggggt ggccttcgcc
     1381 cagaatttcg ggttcgccgc gctgttgttc ttccttacgc tctatctaca gaacatcctc
     1441 ggctactcgc ccctccaggc cgggctggta ttcctgacgt tctcggcgat cctcgcgata
     1501 gtcgactcat tcgcgggccg gctggcagcg tcggtagggg cgcgcctgcc gatggtggtc
     1561 ggcatggcgc tgtgcgcagt agcgttcctg ctgttgttcc tactgaggcc tacgagcggc
     1621 ctcaccatcg ttattgcagc gctcctggta gcagggatag gtcaggcgct cgcctacacc
     1681 gtctccacta cgggaggcat gcaagcgatc ccagaagcca aggccggcgc cgcctcagga
     1741 atactgtcga tgttacggct tgtgggtgcc gtcttcggca tagcggtgac gggagcactc
     1801 ttcaaagaga gggagaatga caaacttgcc gagctgctca cggcttcggg agccaacctc
     1861 gactcgtcgg accgtgcgga gatgcgcggg ctactgtcgg gatcagaagc cgcccagaat
     1921 aagcttgccc ggatcgcacc aaatgttgcc catcagatcg aacagttggt acgcgaggcg
     1981 tttgtgtacg cgttcgatgg cgcgatgctc ttgtgcatgg ccgtgtcggt ggctggtgtt
     2041 gcggccgctc tcctagtggc cagcaagagc ccgcgaccgc agcgcaccgc tggccccaca
     2101 ccagagagcc ctacacctgg aactgcacag agctagcgac ggagcgcctt ttggaggcta
     2161 accccctcag atcggctgtt ccggggtgac cgcttggcga tggcttcgtg agtggcagcg
     2221 cgcagggatg taggagagac tgcaccgcga gccgatggtt ttgcacaggt tttactgctc
     2281 cgccaatcca gatcggctgc tcctgcaggc tcttcacgcg cgctgcgtac ttagtggcta
     2341 gcatctgggg attggccccc ctacagtttc gccacccctg cgcgggcgca acggctggga
     2401 catggtacgg caccacctag agttgcggtc gccgctgcac ctcgttgatg cctgccaccg
     2461 tgatcctaaa cgtttcctcc ccttcatccc cggcacgcgc caggtaccct acgtccacca
     2521 gataacccag cgtcgcctca aactcgcggg agtcgagatc gaagcctgct tcttgcgccg
     2581 cggcgctcgg agtaacgtca gttgtggggc tgcctccggc caactcatcg aacgctcgca
     2641 ggagctggtg ttggttgccg ctgagctcgc tctccatagt agtgtgccgc ctttccctgt
     2701 ggccatcgcc ttgccgacat cttacgtcat tggggcactg cgtatgcctg gtctatgaga
     2761 ctcacccgca cctctacgcc tgggtgggga ctcccggctg catagggagg cgccgggcgg
     2821 gaagcgaact actatcgcct tcaggtgggt ccacggaagt taggacaacg ccgaaattga
     2881 tgttccgcgt ccgcctcgac cccgacggcg tggcgacggt gcgactggtg gcgtagcatc
     2941 cgctaggtct gtggaatggc gaagcttaac gtacgcggcg taagtcgggc ctgaggccga
     3001 ctccgctaca gcatgaggtc gattagatac gggccctcgg aactcagacc ggcggcgagc
     3061 tggcgctcga gctcctccat cgtcttggcg cgtcccgcct ccacgcccat gccgcgggcg
     3121 aggccgaccc agtcgagtgc cggccggtcg agctccatca tgtcgagcgc cctgcgtccg
     3181 gcatcgcccg ccccgaagtt cgtcagctcc tgtttgagga tcgcgtagga gcgattgttc
     3241 agcaagagtg tggtgaggtc gaggccctcg cgtgcctggg tccaaagggc ctgcacggtg
     3301 tacatcgcgc tgccgtcagc ctgcaggcag atcaccttgc gatccgggca agcgaccgcc
     3361 gccccggtcg cgagcggcag tccgataccg atagcgcctc cggtgacctg taggtagtcg
     3421 tgcgcggcag cgccccgcgt ccgcgacatg aaagcccgag cggcactgag cgcctcgtcg
     3481 acgacgatcg cgccttccgg aagtagggtc ccgaccgctg ctgcggccgt ctctggggtg
     3541 atcgcttcac ccgagggtgg tgggtacttc tcttgcgcct ggacgggggc attctgcggc
     3601 gcgtcgagct cttcggcgag tgcctccagc gtgcccacga tgtcctcgcc gggccttgcg
     3661 agcgtgtggg tctcgcaagg cggcgggctc agcgcgctcg gcttgtcggg gtaagcgaag
     3721 aaggcgaccg gtgcctcgga accgaccagc acgaggtggc gcgcgtcggc gagggtcgct
     3781 aaagcgtcgt cgaccgggta ggggaggaag tcgatggcga cacgccccgc cccgcgttct
     3841 acccgcgcgt tgagcgtctg ggcgagcaga cgcgcgccgg tcttcgcggc gatgcgaccc
     3901 gctagcatca ggccgggttc gcgcagggca cgtccgccca gaaacaggac cgcgggttcg
     3961 ctggagcgca aggcccgagc aacatcagag attgcctcgg gtgcgaccgg ctcgcgaccc
     4021 tcaacggggg gcagcggggc cggcctgtcg gcctcgttcc aggaggcgtc ggctgggagg
     4081 atcagggtcg cgacccgacc cggcggggcc atcgccgcgg ctacagcatc tgcgccgtcg
     4141 cgcgcaaccg agcgagagtc ggctgaggtc atgacccagt ccgagaaggg tcgcgcggcg
     4201 ccctcgacgt cggaggtgag cggcgcgtcg tactccttgt ggtaagtcgc gtggtcgccg
     4261 acgatgttga ccatcggcgt gttggctcgc cgggcgttgt gcacgttggc gagcccgttg
     4321 gcgagtcctg gcccaaggtg cagaagggtc gaggccggct tgtccgccat acgcgcgtag
     4381 ccgtcagctg cacccgttgc aacgccctca aacagcccga gcacgcaccg catatccccg
     4441 acctgatcga gcgccgcgac gaagtgcatc tctgaagtgc ccgggttggc gaagcagacc
     4501 tccacccccg cgccggccaa ggtctcgacc aagctttcag ccccattcat ttgttcctcc
     4561 cctacactcc cgtcgatcgg cgtacctgac taatcttagc ctccgagcct agctgaaaca
     4621 gctgcgccga cggaggcgga gcaaccttct tccgccttca ggtgggccca cgcaagttag
     4681 gacaacgccg ggtgcgaagt tccgcgctct gctttctgga gtaggtgagg acgtgctcct
     4741 cgaaagcgtc ctctagtggc tcggctccac gctttctccc tgctgaggaa tgacgccgag
     4801 ctgctgcatc atgccgaggt tgtcccctac ggaccagtgt tccaccaccc gcccatcgga
     4861 gatgcgcacg acgtcaatga gccccataga cacccgccgg ccggggggat gcccataaac
     4921 tcgccctgat ggctaccgtg aaaggtcctg cgcgtcgcca ccttgtcccc ttcggccatc
     4981 atgtcctcta cggtgaaata cgaatccgga aaggcttgac gaaacatggc cgtgagctgc
     5041 ttgacaccct cacagttgga aggcactccc ggcggggcac tatggtttac gaactcgggg
     5101 gagcaaattt catccaaggc gtcgatgttg cctctgctct gcacttcgtc gacgaagcgg
     5161 cgcactaggg ccttgttctc atcggcggac actgcttcct cccttccccc tgggtgggcg
     5221 aacttgtgga catcatgatg ccacccgtag acacctcgcg cgaacttcgg agaaaacccc
     5281 ttcccggaca ttcgtaaaga agccttcggt cgatgcacgt tactcctacg ggtggcatca
     5341 tgctcatgtt gccaacccct tggggaaaga acgcgagttg gcaaggagga agctccatgt
     5401 cggtagaaga gaacatggca ttggcccgcc gtttcttgga gtcacaggtc aaaggagatc
     5461 tggaggcgat ggacgagatg atggctcccg acttcgtcgg ccacaccaaa ctgcttcccg
     5521 accaacagcc cgaccgcgaa ggccaaaaat gggcgtacgc ccaattttct gccgccacct
     5581 ccaacgccag catagacttc gaggaccaga tagccgcggg ggacaaggtg gttacgcggt
     5641 tcatcgtgca cgccacccac gatcgagggg agctcatggg tgttgcgcct agcggcaagg
     5701 aattggttta tatgcccatt ggcatccacc gcatagaggg gggcaagatc gccgagcagt
     5761 ggggcggggg cacaggcctc tccgaactga gaggactgcg gctagagcaa gaagagcgcg
     5821 agcgcgagcg cgtagagcag gaactcaggg tagcgaggcg catccagcaa gcctccctcc
     5881 ccaaggtggt gccccgactg gagggctggc agatcttccc ctactactgg ccagccagag
     5941 aggtgggagg agatttctat gatttcttcg aactagtaga cgaccgggta ggagtagtgg
     6001 tgggggatgc cacgggcaag ggaatgcccg cggctttagt ggcggaggcg acctccaaca
     6061 tgctcagggc ggtagcccag gccttgggtt cctcctcgcc tagcgagata ttagcccgag
     6121 tcaacgagac gttgctggct cgcatccctc ctaacatgtt cgctacctgc ttttacgcca
     6181 tcctagagcc caagagcggc cgactccact acgccaacgc aggccacaat ctcccttgtt
     6241 gccgccacgg ggagagacag accgacgaac tcgtggcaag ggggatgcct cttggactga
     6301 tgccggggat ggattacgaa gagaaagagg ccgtccttgc tccaggggac tgcgcactgt
     6361 tctacaccga cgggctaata gaggctcata atccccaagg ggagatgtac ggaactcccc
     6421 gtcttcggaa cctcctgagc gaacgtcccg agggcgggac tggtctccac gccaccttga
     6481 tgaaagagct tgagcgtttc actggagagg gctgggagca ggaggacgat gtcaccctcc
     6541 ttaccctgga gcgctccgct gcctgacgct cagcttctgc gaacgctcct ttgccgaagg
     6601 taggttgaat aggggatgaa gtcgtcgcga ccgatcccac atcggccaac gccgattgcg
     6661 acaacggcgc tggtgccgtt catggtatgc ctcaatgaag atggccgttc aaggatccgg
     6721 ctgatagcct agggtgaggc ccgcgaggag gcggaagcgg gcaccgttgt cgcgactgtg
     6781 ccgcccgagg caatcgaacg ccgctgcact aacttcaggt tgtaggcgca tggcgactgg
     6841 cccagctggg taatataggg tggaccggaa aggtggccga gacatgcccg agcccgtaaa
     6901 cgtgaagtgt ccttcgtgcg acgagaacct tgcggccacc gacgaaagcg acctcgtgaa
     6961 cgtcctacag gagcacctat acgaagagca cgccctggaa atgccgcgtg agagggtccg
     7021 cgagaacgtc gtagctcagc tgcgagattt cgacggctac aacgctcgac ctatcaccgg
     7081 ggcctgatag atggttcagc cgtgagggaa agatatcggc gggggagggc gccgtaacgg
     7141 tgggaacatc tagccacgca ttctgtaata acgtgtcgac caaccatggg gaggtcgcac
     7201 gtcgatggtg acgctcgtgg ccgtcctggc cgtcgccgtg gcggcggggg ctgtcatcca
     7261 gggttctata ggcttcggat acgccctcgt agcggtgccg gcgatggttc tgctgttgcc
     7321 gtgggcagta cccgtcacgc ccctctttct ggctctccct atgaccctgt tcatgagcgt
     7381 gagggaatgg cgttccatcg acctgagcgg cttcgccctg ataactgccg ggcgcctgct
     7441 cggcacggtc gctggtgtcg tgctcttggt cttggcgccg aagggctacc tttccattgt
     7501 cacggggcta ctaatcctga cggtagcact cgggagcttc ctcaaaccaa ccttcgagat
     7561 gaacaagcgg acccggcttg cagggggagt gtcctctggt gtcgtgggca ccgtggcggc
     7621 actcggcggt acgccgctgg cgctcgtgta ccaggaccgt tcgggagcag agctacggtc
     7681 gacactggcc atctcgttcg tggtgggaat cgtcatgtcg ctggttggcc tagggttagc
     7741 cggcaaggtc gaagggcggc acgttgtctt ggctctcgaa ttgctgccgg gactgctcgt
     7801 tggtttgtgg gccagcgagt gggtcgccaa gcgtctcgac gagcgttggt tgcgtccgac
     7861 ggtgttgatg tttgcagcgg tcgcgggggt agtgattatc ctcttgggtc ctggcggttg
     7921 aagccttacc gttcgctatg gcgaggattt caacctgcag ccttcaggtt atgagccgac
     7981 ccagtatcct accgtggggc tcttggtcgt gctcggtaga ggcgccggcg gaggcgagtc
     8041 accctggagg gaggccgtac aattcatacc cctagtggcc ctgcgacccc cgcagtatgc
     8101 atgcagcggc gcggctaccg agcctcggtg aaacgatcgg cgaggagcac agcgtgcgtt
     8161 acggcgtgag cgtgcccaac ttcggggtag gggtggacgc ccgagtgatc gcggagttgg
     8221 cccgtgaggc cgaggaggca ggctgggacg ggttcttctt atgggaccat ctacttgcct
     8281 tctcccctgg acccgtgccg gtagtcgacc cttgggttgc gctgtccgca gtcgccctta
     8341 gcacgagccg cctaaggctg ggtccgatgg tcacgcctct gcccaggcgc cgtccgggga
     8401 agctggcgag ggagactgtc tccctcgatc acctctccga cggacgattg atcctgggcg
     8461 tcggcatcgg cgccatgccc tacgagtacg actacctggg cgaggaggcg gacacgcggg
     8521 tgcggggtgc catgctcgac gagggactcg aggtgctcac gggcctgtgg agtggcgaac
     8581 ccttcggcca ccgcggggag cactacaggg tcgcaggcga tccgcccgag caggactggc
     8641 gggccgtctt ctaccctccc ccgctgcaac gcccccgcat ccccatctgg gtgggcggca
     8701 cttggccgat aaaggccccc ttccgccgtg ccgcgcgctg ggacggggtg ttcccgatga
     8761 aggtcgaggg cggacgcata gtgccaatga cgccagagga cgcgcaggag gtggcgcact
     8821 acatcgcggc ccaccgcgcg ggggacggac ctttcgagct ggtggtagca ggggagacgc
     8881 cgggcgacga tcggaagaag gctgctcgca tcatgtcggc ctttgaggag gctggcgtca
     8941 cttggtggat agagagcatc gacacctggc gcttcggatg gacagaaggc gagacctggc
     9001 cgtccgagga gatgcgccac cgagtgcggc gaggaccgcc cgaagcatga gacacgaaca
     9061 ggcgccagcg gtgccgttat cgcgaccgac tgtgtgtcgc ccgacgccga ttaggacaac
     9121 gggaccgttg ccctttacgg tgcgcctcga ctcagagggc gcgatagagg cacggctcat
     9181 cgcgtaggag gcgcgaggtc cgtgaggagg cggagtcaag gacaggcgta gcagccgcgc
     9241 tcgagcaggt ccaggtccta gaaggggtcc cggccttctg ctaccgtcca gctaccaggc
     9301 cggggttctg gcaacgctcc cctgatcact tagtgcgcct ctgccttggt cctgtggcac
     9361 gtgcattcgc ccggaccggg gcggagcgac tcagggcgga tagggtgttc tggcgctgcc
     9421 actgtatcct tgggggaagc aaagtgagcg cgaaaggaga agaggatgga tctggagacc
     9481 aatgctgcag gcaagcagga gattctggcg tccggatctc agggaaagcc gatagttgcg
     9541 cggacatggt tcgggcgaac ccgtagggag caagcggacg agtacctcaa gtataactac
     9601 gagaacggtg ttctcgaggt cgagaagacg aggccgacgt cttgcgcctg tatccctggc
     9661 ccgatgccac tgtggccggc gaggtggaag cggaagagct ccggaaggtg gcgcaagcac
     9721 agtggccgaa gccgtgggct gcgtggggct tcgacgccgc cgacttgggc ggcccaggaa
     9781 ccgcaaccct cgccgtccta gaaggcgacg cggtcgcgca cgtggagcgc accttgggcc
     9841 ggcaggtgga gtggcggcag acgggagtag ggctacgcga ggccgtcggt agggcactcg
     9901 gctagttcgt cctggcgcat aagggcaaga aagcggtcag ggcagcggtc gtccgctcct
     9961 ggtctgggcc gaaagttccg ctacgacccc gaagtggtcg ctcgcccaca cgccgtccac
    10021 aggttcgtcg aagatgcgct cgcaggagga aacatcaagc gtaggtccgt ggtggccgca
    10081 gcgcaccatg atgtagtcga tcctggggcc cagctccagc ggccagttgt cgccactagt
    10141 caccagcgga ttgcggggcg taaacgtgtg accgggatcc tcggggtgtg tgctctccca
    10201 ggcgtcccgg tagcagacgc tcgtgtcgcc caaagactgc aacccctgcc accctcaggc
    10261 ttctgggact ggtggcggtg gacgtagctg acgacccgcc tcatgtgttc gatatcgtcg
    10321 ttcgagtagt cagtcctgtt cttcccgagg atctggacaa tctttcggcc cgacttgtgg
    10381 ccctttgact cgcccccgcc gtcgctctgt ccgac
//
LOCUS       DATMJA010000253         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_863375, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000253 DATMJA010000000
VERSION     DATMJA010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863375"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VNT12_06070"
     CDS             <1..1041
                     /locus_tag="VNT12_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWI45717.1"
                     /translation="FEGEIPFEKVNVHSIVLAEDGTKMSRSKGNTIDPLELFDEYGTD
                     AVRFGLLYQSSTQDFPYSRERAEMGRAFVTKLWNAMRFVLAYPEAEEPGELSPSDRWI
                     LSEFSRTVMEYDAYLEECEFSEAMRRIYSFAWNQFADWYIEIAKAAPSTATPRILREV
                     FHGILKLLHPVMPFATEEMASVMGEQGLLATQSFPEPDPALEDEEADRLLDRTRRAVS
                     AVRSFRAESKVDGQLEGLVPQGIDEGVFAALAGVRPVEAGDGASRASLPAGDVVVEIL
                     LSEEMRRGEIERLRREISRAEQEVARAQSKLANEKFVTNAPEKIVAGEREKLDVNSRM
                     LETLSRRLDEYI"
     gene            1060..2280
                     /locus_tag="VNT12_06075"
     CDS             1060..2280
                     /locus_tag="VNT12_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HWI45718.1"
                     /translation="MTSFAQVCAELDRRRHVTLGFERIEALLTAMGNPERAVPGLVQV
                     VGTNGKGTTAFALATALEEMGYASGAYLSPHVLSYTERVVLRGGQVSEEDFAAAMGET
                     IELADRNSILASQFELLTAGALGMFAAAGLSWAVLEAGLGARHDATSSVRPDAVVLTN
                     VSIDHTEYLGETVEKIAEEKLASMKPGATLYLGTDERLLVDLAHSACRRVGARLVLPG
                     GASENASFSGLAPYEADDVRLGIAVAESLLERSLPAEVEQKVALAVGEALPGRFEVHE
                     VEGVPVVVDGGHNAAGLSAALAGMRAVYGERPLGVVFGVLREKDAASMLARLKGETDV
                     IVLTRPESERAADPAHLEDDLLDCGERRAMVVENPLEALGVAVEAVRSGGGVVLVTGS
                     LSTAAPLLRWLREA"
BASE COUNT          444 a          710 c          768 g          410 t
ORIGIN      
        1 ttcgaaggcg agatcccctt cgagaaggtg aacgtacact ctatcgtgct cgctgaggac
       61 gggacgaaga tgagccgctc caagggaaac accatagatc cactggagct attcgacgaa
      121 tacgggaccg acgccgtacg cttcggcctc ctctaccagt cctcgaccca ggacttccca
      181 tactcccgcg agcgggccga gatgggccgc gccttcgtca ccaaactgtg gaacgccatg
      241 cgcttcgtat tggcctaccc cgaggctgag gaacctggag aactctcgcc ttctgaccgt
      301 tggatcctct ccgagttcag tcgcacggta atggagtacg acgcctacct cgaggaatgc
      361 gagttctctg aggcgatgcg acggatctac tctttcgcct ggaaccagtt cgccgactgg
      421 tacatcgaga tagcaaaggc agccccctca accgcaacac cacgcatctt gcgcgaggtc
      481 ttccacggta tcctgaagct cctgcacccc gtcatgccct tcgcgacgga ggaaatggcg
      541 agcgtaatgg gagaacaggg gctgctcgca acgcagagct tccccgagcc tgaccccgca
      601 ctagaagacg aggaggccga ccgcctgctc gaccgcacca ggagggccgt ctcggcggtg
      661 cgctcgttca gagcagagtc gaaggtagac ggccagctcg aagggctagt gccgcagggc
      721 atcgacgagg gcgtcttcgc cgcgctggcc ggggtgagac ccgtggaggc aggagacggt
      781 gcgagccggg cttcattgcc ggcgggggac gtagtcgtgg agatcctgct ctcagaggag
      841 atgcgccgcg gcgagataga gcgcctgagg cgggagatct cacgcgccga gcaggaagtg
      901 gcgcgcgcac agagcaagct cgccaacgag aagttcgtga ccaacgcgcc ggagaagatc
      961 gtcgccggcg agcgcgagaa gctggacgtc aactccagga tgctcgagac gctctctcgc
     1021 cgtctcgacg aatacatcta ggagtcggcc attcctacca tgacgtcttt cgcccaggtc
     1081 tgtgccgagt tggaccggcg ccgtcacgtc accctcggtt tcgaacggat agaagccctc
     1141 ctcacagcta tgggcaatcc tgaacgcgcc gtgcccggcc tggtgcaggt cgttggcacg
     1201 aacggcaagg gaacaacggc cttcgccctc gctacggcac tggaagagat ggggtatgcg
     1261 tcaggcgctt acctctcacc gcacgttctc tcctacaccg aaagggtcgt gctccgcggc
     1321 ggccaggtat cagaggagga ttttgcagcc gccatgggag agaccatcga gctcgcggac
     1381 agaaacagca tcctcgcttc gcagttcgag ctacttactg ctggcgccct cgggatgttc
     1441 gcagcggcgg ggctgtcgtg ggcagtcctc gaggccggcc tcggcgcccg ccacgacgcc
     1501 acctcttccg tcaggcccga cgccgtagtg ctcaccaacg tctccataga ccacaccgag
     1561 tacctgggtg agacggtcga gaagatagcc gaagagaagc tcgccagcat gaagcctggc
     1621 gcgaccctgt atctcggtac ggacgaaagg ctacttgtcg accttgcgca cagcgcgtgc
     1681 cgccgggtcg gggcgaggtt ggttctgccc ggcggagcca gtgaaaatgc ctcattttcg
     1741 ggcctcgcgc cgtacgaggc agacgacgta cgactgggga tcgcggtcgc cgagtcattg
     1801 ctggagagga gtctccccgc cgaggtcgag cagaaggtcg cccttgccgt cggagaggcg
     1861 cttcccggac ggttcgaggt acacgaggtc gagggcgtcc ctgtggtggt cgacggtggg
     1921 cacaatgcgg ccgggctctc ggcggcgctc gccgggatgc gcgccgtgta cggcgagcgt
     1981 cctttgggtg tcgtcttcgg tgtcttgcga gagaaggacg ccgcaagtat gcttgccaga
     2041 ttgaaaggtg aaaccgacgt tatcgtgctg acgcgccctg agagcgagag ggccgccgac
     2101 cccgcacacc tcgaggacga cctcctcgac tgcggtgaga ggagagccat ggtagtggaa
     2161 aaccctttag aggccctcgg cgtagccgtg gaggcggtaa gatcaggggg aggagtggtg
     2221 cttgttacgg ggtccctctc tacagcggca ccgctattgc ggtggttgcg tgaagcataa
     2281 gggcgcctgg cagctcatcg gtcttatgtt ggttatcgcc gtggtctctt tt
//
LOCUS       DATMJA010000254         6219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_212213, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000254 DATMJA010000000
VERSION     DATMJA010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212213"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VNT12_06080"
     CDS             <1..389
                     /locus_tag="VNT12_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HWI45719.1"
                     /translation="YSHVMHIVSTVEGDLREDLTALDALAAAFPAGTVSGAPKVRAME
                     IIDELESTRRGPYAGATGYYGVDGRLDTCITLRTALLKDGVAYFQAGGGVVADSVPSL
                     EYEETRNKARAMEQALEVARTREMWL"
     gene            519..1121
                     /gene="plsY"
                     /locus_tag="VNT12_06085"
     CDS             519..1121
                     /gene="plsY"
                     /locus_tag="VNT12_06085"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HWI45720.1"
                     /translation="MLGILLVLFGYVLGSVPTGYLVGRALGVDVRKVGSGNIGTANVL
                     RAAGKWAAVLTLAGDMLKGLVPVVVARLAVQNEWLIAAVAFATLIGHCWPVFLRFKGG
                     KAVATGAGTTIGLAPLIGLCLFAFWWAVVLLSRYTSLGAIAVMVISPLVFWLSGQPLP
                     YVLYTIVGGALVLWRHRENARALLQGTERKVGQRAEEEQS"
     gene            1122..3389
                     /locus_tag="VNT12_06090"
     CDS             1122..3389
                     /locus_tag="VNT12_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HWI45721.1"
                     /translation="MLGEAYNDKKILLTGGTGFLGTALIEKILRSLPDLGCLYLVVRP
                     SRGKSAAERFEKDVLGAAAFRGLREKLGDDFEDQVAKKVRVLEGDVHAPSLGLGEEGL
                     TELSREVDVVIHSAASVIFDAPLDAAVGSNVRGTLAVLKLARTWEKLPLFVHISTAYV
                     AGTSKEDAPEAPPGPSSPNGTTLDAREEVLGLEAVVAEVDEASQERSLLRRFETEARR
                     ELGMVGEEEEVAERVDQLRRAWMRERLVERGTLRARELGWNDVYTFTKSLAERMVVAE
                     RGETPLVIMRPAIIESSHKEPYPGWIQGTRMADPIIMAFAKGILREFPGNPKSYVDIV
                     PVDHVVNAILAAGVRRPEEPEIFQVASGERNPIRYRDLYDIVRDYFVQHPLRDSSGRP
                     IQVPEWSFPGRRRVEGQLKMELAGLKVAGILAARMPEGHMVSDARQRIARAEKRAKMS
                     LYYSRIYGAYANMTSTFASSRTEALYTALPPEDREAFPFDIADIDWRAWMQGAHLPAL
                     TTRPSRKKRKKTTAEKPGEVAAIFDVDGTIVGSNVVSYFAWLRMRELPAALRPLWLAG
                     FLTKVPYYWGLDKLSRAHFNRAFYKNYAGWKPSRARHLGQESFAGFTLDRIFPDALQQ
                     LRDHKAAGHRVVLLSGALDFLLEPMKDLADDVLCSTLAQENGAYTGELTGAPVAGDAR
                     ARMLASFARRRGLDLSRSYAYADSISDLPMLEAVGNPVAVNPDRRLGTAAKARGWKVE
                     HWDKNGSADGVAKKI"
     gene            3391..4599
                     /locus_tag="VNT12_06095"
     CDS             3391..4599
                     /locus_tag="VNT12_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWI45722.1"
                     /translation="MQALVYRKSIPRYLLMRAGSRRIKSLETSRFSPLQLEDAPDPGL
                     PTPQWVRVKPLLSGICGSDLGTLSSENSPYFSPITSPPFVMGHEVVGEVTEDNSGFRA
                     GERVVLEPALGCAVRGIDPPCPYCASGRHALCLNVARGEISPGIQTGFCRDTGGGWSQ
                     GTLVAHPSQLHRVPDAVTDEAAVAIEPLACAVHAALGAAPAPGDTTLVVGAGSVGLFT
                     VAALRHLTDAGHIICVAKHERQRNEAIRLGATEVVHPNETYTKLPEMLGTASYKPELG
                     KPVVMGGAGKVFECVGAPSTMEDALRLSRPGGKVTLVGMPGSKSTLDLTALWYKEVRL
                     AGSYAYSVEEHDGKQTSSFQLALSIAPEIKLETLVGPHFRLEDYREAIAAARSAGREG
                     QVKVVFDHKR"
     gene            4734..5333
                     /locus_tag="VNT12_06100"
     CDS             4734..5333
                     /locus_tag="VNT12_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HWI45723.1"
                     /translation="MAEGRGEIAGYEFGTRSVVRSPLGIEDLDLLKQTVLFGEEDEEH
                     LRMAGDVLEDQVEDVLDVWYGFVGDHPHLIRYFSDAEGEPDTEYLGRVRGRFGQWILD
                     TCRRPYDREWLDYQQEIAMRHTRDKKNQTDGAYETPDHIGLRYMVAFIYPITATIKPF
                     LAKKGHSAEDVDKMHQAWFKSVVLQVTLWSYPYVKNGDF"
     gene            5335..5955
                     /locus_tag="VNT12_06105"
     CDS             5335..5955
                     /locus_tag="VNT12_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWI45724.1"
                     /translation="MGSVFDLFAQHDDVDIKLVAALERLSQVFRVRLREEAWERDLSP
                     TQAQFLIYLLYHDVELRRVSQLAREFDLTKATVSDAVASLETKGLVQREQWPEDRRVV
                     TLRLTPDGEKLATTLSEWADPVREHLEHFSPEEREAVMRFLIELIGSLQRSGLITVAR
                     MCVTCRFFRQDLHPGEASPHHCGLLDVPLGGADLRVDCPEHEPAVG"
BASE COUNT         1157 a         2000 c         1968 g         1094 t
ORIGIN      
        1 gctactcgca cgtcatgcac atcgtctcta ccgtcgaggg agatctgcgc gaggacctga
       61 ctgccctcga cgcgctcgcg gcggccttcc ccgctgggac cgtctccgga gcgcctaagg
      121 tcagggccat ggagatcatc gacgagctcg agagcacccg ccgcggaccc tacgcgggcg
      181 ccacaggcta ctacggcgtc gacggccgcc tcgatacgtg catcacgctt cgcacagcac
      241 tcctcaaaga cggcgtcgcc tacttccagg ccggcggcgg cgtcgtcgcc gactccgtcc
      301 cttcgctcga gtacgaggag acccgtaaca aggcgcgggc gatggagcag gcgctcgagg
      361 tcgcccgcac ccgcgagatg tggttgtagc cgcacgcagc tagccgcgcc gccagcactc
      421 agctatcggc agtcagctgt cctgtacggt ttgggtccgt cgcttagccc gtacgcagcg
      481 acgccctcgg gcacttgttc cgctatactt tctccgccat gttggggatt cttctcgtgc
      541 tgttcggcta tgttcttggc tccgtgccga ccggatatct ggtcgggcgg gcactcggtg
      601 tggacgtccg gaaagtgggc agcgggaaca tcggtacggc gaacgtgcta cgggcagcag
      661 ggaagtgggc cgccgtactc accctcgcgg gcgacatgct caagggtctc gttcctgtgg
      721 tggtcgccag gctcgccgtg cagaacgagt ggctcatcgc cgccgtcgct ttcgctacac
      781 tgatcgggca ctgctggccc gtcttcctac gtttcaaggg cggaaaagct gtggccacag
      841 gcgctggtac gactatcggg cttgcgccgc tgatcgggct ttgcctcttc gccttctggt
      901 gggctgtcgt gctccttagc cgctacactt cactcggcgc gatcgccgtc atggtcataa
      961 gccccttggt gttctggctc tcaggccagc cgctccctta cgtgctctac accatagtcg
     1021 ggggcgcgct cgtcctgtgg aggcacaggg agaacgcgag agccctcctg cagggcaccg
     1081 agaggaaggt aggccagcgg gcagaagagg agcagagcta gttgctcggc gaggcgtaca
     1141 acgacaagaa gatactgctt acggggggga cgggcttcct cgggacggcg ctgatcgaga
     1201 agatactgcg ctccctcccc gacctcggat gtctctacct cgtcgtcagg ccctcccgcg
     1261 gtaagagcgc cgcagagagg ttcgagaagg atgtcctcgg cgctgcggct ttccgcggtc
     1321 tgcgcgaaaa gctgggcgac gacttcgagg accaagtcgc gaagaaggtc cgcgttctgg
     1381 agggggatgt acacgcgcct tcgctagggc ttggcgagga gggcctcacg gagctctcgc
     1441 gcgaggtgga cgtcgtgatc cactctgccg cttcggtcat tttcgacgcc ccgctcgacg
     1501 ccgcggtcgg ctcgaacgtg cgtggcactt tggcggtcct gaagctcgct cgcacctggg
     1561 aaaagctgcc gctgttcgtt cacatctcca cggcctacgt ggcgggaacc agcaaggagg
     1621 acgcaccaga ggcgccgcca ggcccgtcct cgccgaacgg gacgaccctc gacgcgcgcg
     1681 aggaggttct tggcctcgag gcggtcgttg ccgaggtgga tgaggcttca caggagcggt
     1741 cgctgctgag gcgtttcgag accgaagcgc ggcgggaact cgggatggtc ggtgaggagg
     1801 aagaggtcgc agagagagtc gaccagttga ggcgcgcctg gatgcgcgag aggctcgtgg
     1861 agcggggtac gctgcgggcg cgggaactcg ggtggaacga cgtctacacc ttcaccaagt
     1921 cgctcgcgga gcggatggtg gtcgcagaac ggggtgagac gcccctcgtc atcatgaggc
     1981 ccgccatcat agagagcagc cataaggagc cctacccagg ttggatccag ggtaccagga
     2041 tggccgaccc cataatcatg gcgttcgcga agggcatcct gcgcgagttc cctggcaacc
     2101 cgaaaagcta cgtggacatc gtccccgtcg accacgtcgt gaacgccatc ctggccgccg
     2161 gggtccgccg tcccgaagag cccgagatct tccaggtcgc ctcaggagaa cgtaacccga
     2221 tccgctaccg cgacctatac gacatagtgc gcgactactt tgtacagcac cccctgcgcg
     2281 attccagcgg acgtccgatc caggtgccag agtggtcctt ccccggccgc aggcgggtgg
     2341 agggacagct caagatggag cttgccggcc tgaaggttgc cggcattctc gccgcacgaa
     2401 tgccagaagg ccacatggtc tccgacgcgc ggcagaggat cgcacgcgcg gagaagcgcg
     2461 ccaaaatgag cctttactac agccgcatct acggcgccta cgcgaacatg acctcgacct
     2521 tcgcaagctc ccgcacggag gccctttaca cagcccttcc gccagaggac cgcgaggcgt
     2581 tcccgttcga catcgccgac atcgactggc gggcgtggat gcagggtgcg cacctgcctg
     2641 cgctcacgac ccgcccgagc cgcaagaagc gcaaaaagac gaccgccgag aagcctggcg
     2701 aggtagcggc gatcttcgac gtagacggca ccatcgtcgg ctccaacgtg gtctcttact
     2761 tcgcgtggct caggatgagg gagcttcctg cggccttgcg tccgctctgg ctcgccgggt
     2821 tcctgaccaa ggtcccttac tactggggac tggacaagct cagccgcgcc cacttcaacc
     2881 gcgcctttta caagaactac gcgggctgga agccctcccg cgccaggcac ctcggccagg
     2941 agagcttcgc cggctttacc ctggaccgca tcttccccga cgcgttgcag cagctcagag
     3001 accacaaggc cgccggccac cgcgtcgtcc tgctctccgg cgcgctggac ttcctcctcg
     3061 agccgatgaa ggacctcgcc gacgacgtgc tctgctccac gctggcccag gagaacggcg
     3121 cctacacggg agagctgacg ggcgcccccg tggccggaga cgcccgcgcc aggatgctgg
     3181 cctccttcgc caggcgccga ggcctcgacc tctctcgctc ctacgcctac gccgactcca
     3241 tctccgacct acccatgctc gaggccgtcg gcaaccccgt tgccgtcaac ccggatcggc
     3301 gcctcgggac ggcggccaaa gccaggggat ggaaggtcga gcactgggac aagaacggca
     3361 gcgcagacgg cgtcgccaaa aagatctagg ttgcaagccc tcgtctaccg aaagtccatc
     3421 ccacgctatc tcttgatgcg cgcgggctcc agacggataa agtccctcga gacgagccgt
     3481 ttctctccgc tccaactgga agatgcccca gatcctgggc tccccacccc acaatgggta
     3541 cgcgtaaagc cgctcctctc cggtatctgc ggctcggatc tggggacgct ctcctcggaa
     3601 aactcgcctt acttctcccc catcacctct cctcccttcg tgatgggcca cgaggtcgtc
     3661 ggtgaggtaa ccgaggacaa ctccggcttc cgcgccggcg agcgcgtcgt cctcgagcct
     3721 gccctcggct gcgcggtccg cggcatagac ccgccttgcc cttactgcgc ctcgggccgc
     3781 cacgccctgt gcctgaacgt ggccaggggg gagataagcc ctggcatcca gacgggcttc
     3841 tgccgggaca ccggcggtgg ctggtcacag ggcacgctcg tcgcccaccc ctcgcagctc
     3901 caccgcgtac cagacgcagt aaccgacgaa gccgccgtcg ccatcgagcc cctcgcctgc
     3961 gccgtccacg ccgcactcgg ggccgccccc gcccctggcg acaccaccct ggtcgtcggg
     4021 gccggcagcg tcggcctctt taccgtcgcg gccctgagac accttaccga cgcaggccac
     4081 atcatctgcg tcgccaagca cgagcgacag aggaacgagg cgattcgtct tggggcgacc
     4141 gaggtagtcc accccaatga gacctacacg aaactccccg agatgctagg aacggcctcc
     4201 tacaaacccg agctcggcaa gccggtcgtc atgggcggag ccggcaaggt cttcgagtgt
     4261 gtcggcgcgc cctccaccat ggaggacgcg ctccgcctgt cgcgccctgg cggcaaggtt
     4321 acgctcgtcg gcatgcccgg ctcgaagagc acactggacc tgaccgccct ctggtacaaa
     4381 gaagttcgtc tcgccggaag ctacgcctat agcgtcgagg agcacgatgg aaaacagacg
     4441 agcagcttcc aactcgcact cagcatcgcc ccggagataa agttagagac actcgttggc
     4501 ccgcacttcc ggctggagga ctacagggag gccatcgccg cggcacggtc ggcgggcagg
     4561 gaagggcagg taaaagtcgt cttcgaccac aagcggtgat ccgatgtaac cccgaaaggc
     4621 cccgcctccg caatccgaca cgccaggtat tgacacatat cttgcatcac tctagtatgt
     4681 taggactcct aacatacttg ggatcaggta ctggcgtcta ggagggagat gagatggctg
     4741 agggacgagg agagatagcg gggtacgagt tcgggacgcg gagtgtggtc cggtcgccgc
     4801 tggggattga agacctggac ctgttgaagc agacggtctt gtttggcgag gaggacgagg
     4861 agcatttgcg gatggcgggg gatgtgctcg aggaccaggt cgaggacgtg ctcgacgtgt
     4921 ggtatggctt tgtcggcgat catccgcacc tgatccgtta cttctcagat gcggagggtg
     4981 agccggatac ggagtatctc gggcgggtgc gcgggcggtt cgggcagtgg atactcgaca
     5041 cttgcaggcg tccgtatgac cgggagtggc tcgactacca gcaagagata gcgatgcgcc
     5101 acactaggga caagaagaac cagaccgatg gagcttacga gacgccggat cacatcgggc
     5161 tgcgttacat ggtcgcgttc atctatccga tcacggctac cataaagccg tttctggcca
     5221 agaaaggaca tagcgcggag gacgtagaca agatgcacca ggcgtggttc aagtccgtgg
     5281 tccttcaggt gacgttgtgg agctatccat acgtgaagaa cggggacttc taggttgggg
     5341 tcggtcttcg atctcttcgc gcagcatgac gatgtggaca tcaagctcgt cgctgcgctc
     5401 gaaaggctct ctcaggtctt tcgcgtacgg ctccgggagg aagcatggga gcgagatctc
     5461 agccccacgc aggcccagtt cttgatctac ttgctctacc atgacgtgga gctcaggcga
     5521 gtcagccagc tcgccaggga gttcgatctc accaaggcca cggtcagtga cgccgtcgca
     5581 tcgctagaga ccaaggggtt ggttcagcgg gagcaatggc ccgaagaccg gcgtgtcgtg
     5641 accctgcgct tgaccccgga cggagagaag ctggcgacca ctctatctga gtgggcggat
     5701 cccgtaagag agcacctgga gcatttctcg cccgaggaga gagaggccgt aatgcgcttc
     5761 ttgatagaac tgataggatc tttgcagaga tccggtctca taaccgtcgc cagaatgtgc
     5821 gtgacctgca ggtttttccg gcaagacctc catcccggtg aagcctcgcc gcatcactgt
     5881 gggctccttg atgtaccact cggtggcgcg gatcttcgcg ttgactgtcc ggagcacgag
     5941 cccgctgtgg gctgatacag aggcttcagg ccacaggagc taatcagcat ctcagccagt
     6001 cagcacgctc cggcttacgc cttcgcttgt cacctaattt tcgcgccaaa agagagggtt
     6061 tgcattcggg agcaagacaa cacaatgccg gcgatctatc ctaactttcg agcctagcga
     6121 tggtcgaaat taaggaggac cgccggccat gatcgagtgt gccctaaact cgtcccagac
     6181 gcaaaccacc tttggacccc tgtgcgcctt aggacacta
//
LOCUS       DATMJA010000255         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_508496, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000255 DATMJA010000000
VERSION     DATMJA010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508496"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VNT12_06110"
     CDS             complement(<1..1220)
                     /locus_tag="VNT12_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWI45725.1"
                     /translation="MRGAREHNLKDITVSMPRDVMVVVTGLSGSGKSSLAFDTIYAEG
                     QRRYVESLSAYARQFLGQMDKPDVDHIDGLSPAVSIDQKTTSNNPRSTVATVTEIYDY
                     LRLLYARAGRPHCHVCGFPVASSTPQQMVEKVLTLPEKTRFMVLAPVVRGRKGEYGRM
                     FKELAESGYARVRVDGELHELPVDLGLDKNYKHDIEVVVDRLVIREGIERRLTDSIET
                     ALKLAEGLVQIETVERDGSGESMLFSESFMCTNCGASIAEIQPRTFSFNSPHGACDRC
                     DGLGSRLEIDPALVVPNEDLSVHEGAIVPWANSQTEYHASVLGALSDKYGIDLNLPWR
                     DLPEEHRHLILFGTDGERIYVSYRNRYNRRRQYMTRFDGVVGNLERRYAETDSEYRRE
                     KIEEFMSHVPCPKC"
     gene            1434..>2626
                     /locus_tag="VNT12_06115"
     CDS             1434..>2626
                     /locus_tag="VNT12_06115"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45726.1"
                     /translation="MILKDFSLILLQELDADCPLPGDAARQVRGLAVDEPVKGWISAD
                     EVAVTARETLDEDFVHEVASAGSPAIVWRLEGEVPAESVGRAAALGVGLFVLTPEVPL
                     RQLFEIFSQDDGLLLLSHGAGRTLLESAGGEITIEELAGRISGMLGRSVVVEDPVGRL
                     IASSGYAKEDQLRATLEEQGLRASKSASIDETRRERRDRYARLPDGYLSVPVERWRVA
                     DGELFWTPIGAGAPAGYLWIDLEGGRLRPEDVVLLYWARRVLEAELGKERIRLETELG
                     ARGDFVDDLVSGHYGSVELLLQRARYLGANLTEGALVMVVDIDDFARYLARRRPKEPA
                     IQELKRRLADAVRLQTRQLFSNFLLGPRSDNVILFLGYAEGEGPEELPEKAQLLAKGV
                     QRYVKG"
BASE COUNT          452 a          833 c          839 g          502 t
ORIGIN      
        1 ttacacttgg ggcagggcac gtggctcatg aattcctcga tcttctcgcg ccggtactcg
       61 gaatcggtct cggcgtagcg ccgctccagg ttgccgacga cgccgtcgaa gcgggtcata
      121 tactgcctgc ggcggttgta gcggttgcgg taagagacgt agattcgctc gccgtcggtg
      181 ccgaagagga tgaggtgcct gtgctcctct ggcaggtcac gccacgggag gttcaggtcg
      241 atcccatact tatccgagag cgcgccgagc acgctcgcgt ggtactcggt ctgcgagtta
      301 gcccacggca cgatggctcc ctcgtgcacg ctcagatcct cgttcggcac gacgagcgcg
      361 gggtctatct ccagtcggct ccccagcccg tcgcagcggt cgcaggcccc gtgagggcta
      421 ttgaacgaga acgtacgcgg ctgtatctcc gcgatggagg cgccgcaatt cgtacacatg
      481 aagctctccg agaagagcat cgactcgccc gagccgtcac gctcgactgt ctcgatctgt
      541 accaggcctt cggccagctt cagggccgtc tcgattgagt ccgtgaggcg gcgctctatg
      601 ccttccctga tcaccagcct gtcgacgact acctctatgt cgtgcttgta gttcttgtcc
      661 agaccaaggt cgacgggcag ctcgtgcagt tcgccgtcga ccctgacgcg ggcgtagccg
      721 ctctcggcca gctctttgaa catcctcccg tactcgccct tgcgcccccg caccacaggc
      781 gctagcacca taaagcgcgt cttttcgggg agggtaagca ccttctcgac catctgctgc
      841 ggcgtcgacg acgccacggg gaagccgcag acgtggcagt gcggccgtcc tgcgcgggcg
      901 tagagcaggc gcaggtagtc gtagatctcc gttaccgtgg cgaccgtgga gcgggggttg
      961 ttggacgtgg tcttctggtc gatggagacc gcaggagaga ggccgtcgat gtggtcaacg
     1021 tcgggcttgt ccatctggcc gaggaactgc ctggcgtagg cggagaggct ctccacgtag
     1081 cggcgctgcc cctccgcgta gatggtgtcg aatgcaagag aagacttccc gctacctgag
     1141 agcccggtca ccaccaccat cacgtcccgc ggcatggaga ccgttatgtc cttgaggttg
     1201 tgttcgcgcg ccccccgcac gatgatctgg tcttccgcga tctcttctca cccctcttct
     1261 cgggacgcct caggcaagta tacggccccc tgatgggagg ccaaagaagc agtttgcttt
     1321 tggctatatt ctagcacccc cggggcctat ttccgtagcc gcgtttgcga ttttctgccc
     1381 ctaggcccaa aataacaagc gccggttaca ccctgttgcg aacggagccc gcagtgatcc
     1441 tgaaggactt ctcgctgatt cttctacaag agttggacgc ggactgcccg ctaccaggcg
     1501 acgccgccag gcaggtgcgc gggctcgctg tcgacgagcc ggtcaaaggt tggatctcag
     1561 ccgacgaagt ggccgtaacc gcccgcgaga ccctggacga ggatttcgtt catgaggtcg
     1621 cctccgccgg ctctcctgcc atcgtctggc gtctcgaagg cgaggtcccg gcggaatcgg
     1681 tcgggcgagc cgccgcactg ggggtcggcc ttttcgtgct cacccctgag gtgcctctcc
     1741 gacagctctt cgagatcttc tcccaggacg acgggctact gctgctctcc cacggtgcag
     1801 gccgaacgct cttggagagc gccggcggtg agatcacgat cgaagagctg gcaggcagga
     1861 tctcagggat gctaggacgt tccgtggtgg tggaggaccc tgtggggcgc ctgatcgcca
     1921 gctccgggta cgcgaaagaa gaccaactgc gcgcgacgct cgaagagcag ggtctacgcg
     1981 cctcgaagag tgctagtatc gacgagactc gccgggagcg cagggaccgc tacgccaggc
     2041 tacctgatgg gtacctctcc gtgcccgtcg aacgctggcg ggtcgctgac ggcgagctgt
     2101 tctggacccc catcggagcc ggcgccccgg cggggtatct ctggatcgat ctggagggcg
     2161 gccgcctgag gcctgaagac gtggtgctgc tttactgggc caggcgcgtc ctcgaagccg
     2221 aactcgggaa ggagcgcata cgcctggaaa ctgaactggg tgccagggga gacttcgtgg
     2281 atgacctcgt gagcggccat tacggctcgg tcgagctact cctgcaacgc gcaagatacc
     2341 tcggcgccaa ccttaccgaa ggggccctgg tcatggtcgt cgacatagac gacttcgccc
     2401 gttatctcgc acgccgcaga ccgaaggagc cagccatcca ggagctcaag cgacgcctgg
     2461 cggatgctgt acgcttgcag acccgccagc tcttctcgaa cttcctcctc ggcccgcgct
     2521 cggacaacgt catcctcttc ctgggatatg cggaaggcga aggtcccgag gagttaccgg
     2581 agaaggcgca actgctggcc aaaggagtac agcgctacgt caaggg
//
LOCUS       DATMJA010000256         8076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_686011, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000256 DATMJA010000000
VERSION     DATMJA010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686011"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..2401)
                     /locus_tag="VNT12_06120"
     CDS             complement(44..2401)
                     /locus_tag="VNT12_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWI45727.1"
                     /translation="MTDPRVTGALRYSQDVAMEGMLHARLLRSPVAHARVVGMDASAV
                     PEGIIVLLPQDVRDLGGYGPQIKDQEVFPQERVRYAGDVVAAVAAETADEAEEAVGLI
                     NVDYEELPAVFDEVEAASAGAILVHETTNISRNDAAYFGIRPQQGTNICHLFRLRHGD
                     VEAGFGEADAVVEETYHTAGATHVPMEPHAAVARWEWDRLEVVTGTQTPFNMRTDLAG
                     LFGIDEEKVRIISPPMGGSFGAKTFVRTEAIAACLARKAKRPVKIVLERDEMWITLNR
                     HPATVRVKVGARSDGTLVASETECWANTGAYADCGPGVAQKMGFAAPGPYRIANVRVD
                     SRAVYTNLPPNGAFRGYGQMQSTWARERTMDLLADKVGMDPLELRMKNLLKDGDKYCT
                     GETMHDVHFERLLQDAADAVAWSESRKNKGLCVMLKGMQTPSRAAIAVEAGDGGTYTV
                     RCATTEMGQGAKTTMRMMAAELLDVGVEKVSCPDPDTDVVPYDTRTTSSRSTHMMGRA
                     LKVAVADLKENGERGYGEVVDEGGLDPDTGQGVASSHWHQGAAAAEVEVDEETGRFRV
                     MKLHAPIYAGRVINRPAAELQNEGSMIMGLGTALFESNEFADGQITNANLSDYNVPAM
                     DDMPATLSHELVEREGGEVQGLGETALPPVPPAIGNALYSRGIHVTELPISAESVLDA
                     VDARDGRGDAWAKAGSEPVSTDGRNDSPGECVFASGTAPEPTTPVNGSDGLSDQGRLA
                     SVRFLLASPSALLLLGTLGMGLLAIALYRLLHRRGEAEKKAPLRS"
     gene            complement(2394..3233)
                     /locus_tag="VNT12_06125"
     CDS             complement(2394..3233)
                     /locus_tag="VNT12_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWI45728.1"
                     /translation="MVMIAPEWHEPRSLDEALSLRAEHGEEATVVAGGSFLGIVMNQR
                     LFAPTMLVALRSVPDLDYIGADSGPLRLGAMTTHRAVERSPVVKEGWPSLAFTFSVVA
                     SPRVRNQATVGGVVADADYASDPPSMLAALEARVVARSVRGEREIPMDVFVLDHYETA
                     LEEDELVTELRIPRSEGRSVYRKFSSRSGEDRPCVSVAAAQEDGGLRVVVGAVAGRPQ
                     YFPEICDLARGERLSGEVATEIGRRYAEEIEPLSDSRGSARYRRRVIAVEVRRALEDL
                     NHD"
     gene            complement(3271..4167)
                     /locus_tag="VNT12_06130"
     CDS             complement(3271..4167)
                     /locus_tag="VNT12_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWI45729.1"
                     /translation="MNKREREKSALGNGQWSAVVWFTEETCQDVAVAGGKGASLARMT
                     AEGLPVPPGFVIPSYVLQESIDAERMLELAASHSTRELQELVAVTEPPREGITAAYEV
                     LIGDRLTATYKLVGNKKVAVRSSAVAEDSEAASYAGQQETYLFVEGGEEVCRRVVDCW
                     ASFFSERALFYRSQKGSLEDLRMAVVVQKMVDPEKSGVIFTVEPVRRRRDRMVVEAVR
                     GIGERVVSGEVTPDHYALDRKGKVKREKIVDDRVLTDEELQRLGDLGRQLEERHGVAQ
                     DIEWAMIGEEIYLLQSRPVTTM"
     gene            complement(4194..4913)
                     /locus_tag="VNT12_06135"
     CDS             complement(4194..4913)
                     /locus_tag="VNT12_06135"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWI45730.1"
                     /translation="MSRIVDLSMPVHRDMVTFPRINPPQMLMFDTWEDFAKGVGADKY
                     GVDKLTASYTIIQSDHAGTHMDAVKHIRPDGPGASEIPLEYCFSDGVVLDFRHKEYGA
                     GISAAEIDEALEKIGYELKEKDIVLIQTGASAYNDDQERYLRDHCGMTRESTLHLISK
                     GVRMMGIDAVTFDPPVWAMFEREQFWEAHRVMWEEDYWHLENLTNLDQLPPYGFKLSV
                     LPIKWVGTTGAPVRAVAILDD"
     gene            complement(4910..5551)
                     /locus_tag="VNT12_06140"
     CDS             complement(4910..5551)
                     /locus_tag="VNT12_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWI45731.1"
                     /translation="MSLIRTVLREQIRELLLERILKGELQPGDRIVELQIAQEFGTSQ
                     APVREALSELQSLGFVVYEPYRGTRVRRITEEELAEIYPVRAALEELAAQEAASRLDG
                     KVGELEREFEAMRDAADRGVLQDLAAHDATFHRLIVEAAGNQVLLDTWRTLRVEARVV
                     VTALKTDIDLQELAELHRPLLEALKEASPEKAGGALRQHFETLRTMMKRGENA"
     gene            complement(5548..7281)
                     /locus_tag="VNT12_06145"
     CDS             complement(5548..7281)
                     /locus_tag="VNT12_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HWI45732.1"
                     /translation="MDTNTNEILGTFHGDENFPIDWKEGEADLFWILDDLHCPNPISP
                     MFFDIGGWWLTCDHMFRRFGTPFASDWIAKRVNGYLYTAAVPADPSAYGEASEYGSRY
                     VPRVPESGEYAGKIGAYLGSVLPHYAYNFLDWWRDRLRPEMDRNFAYLDGQDTESMDM
                     VELAVLLEDAIDIHDRHWKIHWMLNFAQFSATTALNATIAEVKGEADPGLVGRLQSST
                     QDRNWDAVEDLWKMKEEIKGDEELRRAFEADTAAGIVEALNGTERGRRFISERLESHQ
                     QEFGYKAIWAHEFQYQTWKENPAPILEAVRGYLATDYDYQEAIQAVRDDLEAAKRDLM
                     DDVPEGEGKDSLQGALDLSLRMNPLTPDHHFYIDQGTNARVRLVLIAIGKQLVEAGVL
                     DDPEDVMFLRYNELRVLMFNPDEIDAREIVSDRRDEQEDAYEVRPPEWIGTATQQALD
                     FPYNALWGFPEKFYREPSEKADEVVGLGASAGVTEGTARVVTSIKESDQVEDGDILVC
                     RMTNPAWVVLFTKVSGLVTDAGGIVSHPAVVSREFGIPAVVGTSDATHKIKTGDRVRV
                     NGSSGVVEILA"
BASE COUNT         1350 a         2651 c         2474 g         1601 t
ORIGIN      
        1 tccgtttacg ttgagtcgaa ccttcatcct acctcctcag tacctatgac ctgagcggag
       61 ccttcttctc ggcctcgcct ctgcggtgga gcagccggta gagcgcgatc gctaggagcc
      121 ccataccgag cgtcccgagg aggagcagag cggacggcga agcgagcagg aatctcacgc
      181 tagccagacg tccttgatcc gacagaccgt ctgaaccgtt caccggcgtc gttggctccg
      241 gggcagtccc agatgcgaaa acgcattctc ccggactgtc attccggccg tctgtagaga
      301 ctggctcgga gccggctttg gcccacgcat cgcctctgcc gtctcgggcg tccacggcgt
      361 cgaggacgct ttccgcggag atcggcagct ccgtcacgtg tatgccgcgg gagtagagag
      421 cgttgccgat cgcgggcggg accggcggga gggcggtctc gccgaggcct tgcacctctc
      481 cgccctcgcg ctccaccagc tcgtgcgaga gggttgcagg catgtcgtcc atcgccggga
      541 cgttgtagtc ggagaggttg gcgttcgtga tctggccatc agcgaactcg ttgctctcga
      601 agagggcggt tcctaggccc atgatcatgg acccctcgtt ctggagttcg gccgcgggcc
      661 gatttatcac ccggcctgcg tagatcggcg cgtgaagctt catcacccga aacctgcctg
      721 tctcttcgtc aacctcgacc tcagcggccg ccgccccctg gtgccagtgc gacgacgcaa
      781 cgccctgacc ggtatcagga tcgagcccgc cttcgtctac gacctcgccg tagccgcgct
      841 cgccgttctc cttaaggtcg gcgacggcca ccttgagcgc ccggcccatc atatgcgtcg
      901 agcggctgga ggtcgtacga gtgtcgtacg ggacgacgtc ggtgtccggg tcggggcaac
      961 tgaccttctc cacgcccacg tccagcagct cggcggccat catgcgcatc gtcgtctttg
     1021 cgccctggcc catctcggtc gtcgcgcaac ggacggtgta ggttccgccg tctccggcct
     1081 ccaccgcgat cgccgctcgg ctgggggtct gcatgccctt gagcatgacg cacagaccct
     1141 tgttcttccg gctctcggac caggccacag cgtcggcggc gtcctgcagg agcctttcga
     1201 agtggacgtc gtgcatagtc tcgccggtgc agtacttgtc cccgtctttg agaaggttct
     1261 tcatcctcag ctccagtggg tccatcccga ccttgtcggc gaggaggtcc atggtgcgct
     1321 ccctcgccca ggtggactgc atctggccgt agccgcggaa ggcgccgttg ggcggaaggt
     1381 tggtgtagac ggcgcgcgag tcgacccgaa cgttggcgat gcggtacggc ccaggggcgg
     1441 cgaagcccat cttctgggcg acgccgggac cgcagtcggc gtaggccccg gtgttggccc
     1501 agcactcggt ctcgctagcg acgagcgttc cgtcgcttcg agcaccgacc ttcacccgga
     1561 ccgtggccgg gtggcggtta agcgttatcc acatctcatc gcgctcgaga acgatcttga
     1621 ccggcctctt cgccttgcga gcgaggcacg cggctatcgc ctccgtcctg acaaaggtct
     1681 tcgccccgaa cgagcccccc atagggggtg agattatcct gaccttctcc tcgtctatgc
     1741 cgaagagccc ggcaaggtcc gtgcgcatgt tgaacggggt ctgtgtgccg gtcacgacct
     1801 ccaaccggtc ccattcccag cgagcaacgg cggcatgggg ctccatcggg acgtgcgtag
     1861 ccccggcggt gtggtaggtc tcctcgacca cggcgtcggc ctccccaaac ccggcctcga
     1921 cgtcgccgtg tcgcagccgg aagaggtggc agatgttcgt cccctgctgc ggtcgaatgc
     1981 cgaagtacgc ggcgtcgttc ctagagatgt ttgtcgtctc gtggaccagt atcgcccccg
     2041 ccgaggcggc ctccacctca tcgaagacgg cgggaagctc ctcgtagtcg acgtttatga
     2101 gccccaccgc ctcctcggcc tcgtcggcgg tctcggcggc gacggccgcg actacgtccc
     2161 cggcgtagcg aactcgctcc tgagggaaga cctcctgatc ctttatctgc ggcccgtagc
     2221 cgccaagatc ccggacgtcc tgcgggagga ggacgatgat gccctccggg accgccgagg
     2281 cgtccatccc caccacgcgg gcgtgggcca cgggcgagcg caggaggcgg gcatgaagca
     2341 tcccctccat cgccacgtct tgcgagtagc gtagggctcc ggtaacgcgg ggatcagtca
     2401 tggttcagat cctccagggc gcgcctgacc tccacggcga tgacgcggcg acggtaacga
     2461 gccgagccgc gcgagtcaga gagcggctca atctcctcag cgtagcggcg tccgatctcg
     2521 gtggccactt cgccgctcag gcgctccccc ctcgctaggt cgcagatctc cgggaagtac
     2581 tggggccgtc cggcaaccgc gccgaccacg acccgtagcc cgccgtcctc ctgggccgcc
     2641 gcaacggaga cgcagggtcg atcctcgcct gagcgcgagc tgaacttccg gtagacggag
     2701 cgtccctcgc tgcggggtat ccggagctcc gtgacgagct cgtcctcttc gagtgcggtc
     2761 tcgtagtggt cgaggacgaa cacgtccatc ggtatctcgc gctctcccct cacgctccgc
     2821 gcaacgaccc gcgcctcaag agccgcgagc atcgagggcg gatctgaagc gtagtccgcg
     2881 tcggcgacga ccccgccgac ggttgcctgg ttcctgacac gcgggctggc gaccacggag
     2941 aaggtgaagg caagggacgg ccagccctcc ttcactaccg gcgagcgctc tacagcccgg
     3001 tgggtggtca tcgccccgag ccgtaacggt ccgctgtcgg cccctatgta gtcgaggtcc
     3061 gggacgctgc gaagggcaac gagcattgtg ggggcgaaga gcctctggtt catgacgatc
     3121 ccgagaaagg agccgcccgc gacgacggtc gcctcctccc cgtgctcggc acgcagggag
     3181 agagcctcgt ctagcgagcg tggctcgtgc cactccggtg cgatcattac catctcaaaa
     3241 atccttccag aaccgcgtca aatagtgcgt ctacatcgtc gtgacgggcc gggactggag
     3301 gaggtagatc tcctcgccaa tcatcgccca ctcgatgtcc tgtgcgacgc cgtgacgttc
     3361 ctctagttgc ctaccgaggt cgcctagtct ctggagctcc tcgtcggtaa ggacgcggtc
     3421 atccacgatc ttctcgcgct tgaccttacc cttgcggtcc agggcgtagt ggtccggggt
     3481 cacctcgccg gagacgaccc gctccccgat gcccctgacg gcctcgacaa ccatccggtc
     3541 gcggcgccgc ctgacaggtt ccacggtgaa gatcacgccg gacttctcgg ggtctaccat
     3601 cttctggacc acgacggcca tccgcagatc ttcgagggag cccttctgcg aacgatagaa
     3661 gagagcgcgc tcggagaaga aagacgccca gcagtccacg acccgcctgc agacttcctc
     3721 gccaccttca acgaagaggt aggtctcctg ctgaccggcg tagctcgcgg cctcggagtc
     3781 ctccgcgacg gcggaggaac gaacggccac cttcttgttg cccacaagct tgtacgtagc
     3841 cgtgagccga tcgcctatga gaacctcgta ggcggccgtt attccctccc tcggcggttc
     3901 ggtaaccgcg acgagttcct ggagctctct cgtggagtgg gaggccgcga gctccagcat
     3961 ccgctcggcg tcgatggact cctggagaac gtacgaggga atcacgaagc ccgggggcac
     4021 cgggagtccc tcggcggtca tgcgggccag actggccccc ttgccgccgg ccaccgccac
     4081 atcctggcaa gtttcctccg tgaaccagac gacagcactc cactgcccat tacccaaagc
     4141 ggacttttct ctttcccgtt tgttcatgtc ctacggcctc tccgccccgg ccctcaatcg
     4201 tcgaggatgg cgacggcacg aacgggggct cctgtggtcc cgacccactt gatgggcaac
     4261 acggagagct tgaagccata tgggggaagc tggtcgaggt tagtcaggtt ctccaggtgc
     4321 cagtagtcct cctcccacat gacgcggtgg gcctcccaga actgctcgcg ctcgaacatc
     4381 gcccacaccg gcgggtcgaa ggtcacggcg tctatgccca tcatgcggac gcctttggag
     4441 atgagatgga gcgtggactc ccgggtcatg ccgcagtgat cgcggaggta gcgttcctgg
     4501 tcgtcattgt aggcggaggc tccggtctgg atcaggacga tgtccttctc cttgagctcg
     4561 tagccgatct tttcgagcgc ctcgtctatc tcggccgccg agatacccgc tccgtattcc
     4621 ttatggcgga agtccaggac aacgccgtcg gagaagcagt actcgagcgg aatctcgctc
     4681 gcgcccggac cgtcgggtcg gatgtgcttt acggcgtcca tgtgggtgcc ggcgtggtcg
     4741 ctctggatga tcgtgtagga ggcggtcagc ttgtccacgc catacttatc cgccccgacc
     4801 cccttcgcga agtcctccca ggtatcgaac atcagcatct gaggcggatt gatgcgcggg
     4861 aaggtcacca tatcccggtg gaccggcatc gagaggtcga cgattctgct catgcgttct
     4921 ctcctctttt catcatcgtt cgcagcgtct cgaagtgctg ccggagcgcg ccgccggctt
     4981 tctccggaga tgcctccttc agcgcctcta gcagaggccg gtgcagctcg gcaagctctt
     5041 gcaagtctat gtccgtcttg agggccgtga ccaccacgcg cgcctcgacc ctcaaggtcc
     5101 tccacgtgtc gaggaggacc tggtttccgg cggcctcgac gatcaggcgg tggaacgtgg
     5161 cgtcgtgggc ggcgaggtcc tggaggacgc cccgatccgc ggcgtcgcgc atggcttcga
     5221 actctctttc gagctcccca accttcccgt cgagcctgct cgccgcctcc tgggccgcca
     5281 gttcttctaa cgccgctcgc acggggtata tctcggctaa ctcctcctcg gtgatcctgc
     5341 gcacccgagt cccccggtag ggctcgtaca ctacgaaacc gagcgactgg agctcgctga
     5401 gcgcctcccg caccggtgcc tggctcgtac cgaactcctg agctatctgg agctcgacga
     5461 ttcgatcccc gggctggagc tcacccttga ggatgcgctc cagtaggagt tcccggatct
     5521 gttcccgcag cacggttcgg atcaggctca cgcgagtatc tccacgacgc cgctggagcc
     5581 gtttacccgg acgcggtctc cggtctttat cttgtgggtg gcgtcggagg taccgaccac
     5641 ggccggaatg ccgaactcgc gggagacgac cgccgggtgg gagacaatcc cgccagcgtc
     5701 ggtgacaaga ccgctgacct tggtgaagag gacaacccag gcagggttcg tcatccggca
     5761 gacgaggatg tcgccgtcct cgacctgatc cgactccttt atggaggtga cgacgcgggc
     5821 cgtaccctca gtcaccccgg ccgacgcccc gagccccacg acctcatcag ccttttcgga
     5881 gggttcgcgg tagaacttct cggggaagcc ccagagcgcg ttatacggga agtccagcgc
     5941 ctgctgggtc gccgtaccga tccactcggg aggccgcacc tcgtaggcgt cttcctgctc
     6001 gtctcggcgg tcggagacga tctcgcgggc gtctatttcg tccgggttga acatcaggac
     6061 ccgcagctcg ttgtagcgga ggaacatcac gtcctcggga tcgtcgagta cgcccgcctc
     6121 gacgagctgc ttgccgatcg cgatcaggac gagccgcacg cgggcgttgg tgccctggtc
     6181 tatgtagaag tggtggtccg gggtgagcgg gttcatccgc aaggagaggt cgagcgcacc
     6241 ttggaggctg tctttacctt cgccttcggg cacgtcatcc atgaggtccc gctttgcggc
     6301 ttcgaggtcg tccctgactg cctggatagc ctcctggtaa tcgtagtcgg ttgcaaggta
     6361 cccccgaacg gcctccagaa tcggggccgg gttctccttc caggtctggt actggaactc
     6421 gtgagcccag atcgctttat aaccgaactc ttgctggtgc gactcgaggc gctctgagat
     6481 gaagcgcctg ccacgctccg ttccgttcag ggcctccaca ataccggccg ccgtgtccgc
     6541 ctcgaaggcg cgccgtagct cctcgtcgcc tttgatctcc tctttcatct tccacaggtc
     6601 ctcgacggcg tcccagttgc ggtcctgggt ggagctctgc agcctaccca cgaggcccgg
     6661 gtccgcctcg cccttgacct ccgcgatggt cgcgttgagg gccgtcgtcg ccgagaactg
     6721 ggcgaagttg agcatccagt ggatcttcca gtgccggtcg tggatgtcta tggcatcttc
     6781 tagcaggacg gcgagctcga ccatatccat gctctccgtg tcttgaccgt cgaggtaagc
     6841 gaagttgcgg tccatctcgg gtctgagcct gtcacgccac cagtcgagga aattgtacgc
     6901 gtagtggggg agcaccgatc ccaggtacgc gccgatctta cccgcgtact cgccgctctc
     6961 cggcaccctg ggaacgtagc gactgccgta ctcagaggct tctccgtaag ccgacgggtc
     7021 ggccgggaca gcggccgtgt agagatagcc gtttacccgc ttggcgatcc agtcggaggc
     7081 gaatggagtg ccgaacctgc gaaacatgtg gtcgcacgtg agccaccacc cgccgatgtc
     7141 gaagaacatc ggagagatcg ggttcggaca gtgaaggtca tccagaatcc agaagagatc
     7201 cgcctccccc tccttccaat caatagggaa gttctcatcg ccgtggaacg tgccgagtat
     7261 ctcgttggtg tttgtgtcca cttgtcctcc tctcctgcag gcccacacac caacacctga
     7321 tcggtgtgca ctccaggttc cgtttcccta atgccaataa ttatcgatta tctttaatcg
     7381 agagtcaagt gaggaagcgg acaagccata tgggaggttc ttctcttcct cggtgtagcg
     7441 actttcgcgc gtgagataga ctccgggcag acacgttccg gcgctgaaac agttcgtagc
     7501 tggctcttta tagacgtcga tcctcaggta actctcagcg aggatcgtgg cagtttctgg
     7561 gcttctcttc atttgctacg tttatcctgc aaaacagcga tttctagagt ggaggcgatg
     7621 ggattcgaac ccacgccctc tgccgtgtga aggcagtgcg atagtttgct agaggtgtct
     7681 ggagattaca aaatacctgc aaatagtcgt atttctgcgt tgacgcattt cccggcattt
     7741 caggagattt acccgggttg ctgcacagac ggtaaggctt gcatgcgcac aaggtccggg
     7801 atgatcgcta ttcgaccaac agcctggagg ccacttatgt gagcttcgtc ttgaaactgt
     7861 ccgaaaagcc cggaaggtgc gtcaaacatg gcctcctggg gcaccgcagc ggccctattc
     7921 gatcggcttc agcccgatat actgtccaga cacaccctct aagcgctact cagatacctt
     7981 ttcggacagt ttcttaggtg aattccccga agttggactt cccgctttac ggagttctaa
     8041 gaggaccgct ggtcctgtaa acctaaagca acgccc
//
LOCUS       DATMJA010000257         2101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_863781, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000257 DATMJA010000000
VERSION     DATMJA010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863781"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VNT12_06150"
     CDS             complement(<1..1746)
                     /locus_tag="VNT12_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45733.1"
                     /translation="MPAMNICTIIAKNYIAHARVLAQSYKEHHPEGTCHVLVIDETEG
                     YIDASLEPFELVTPAQLGIDDYEHMAAIYDVLELSTAVKPWLLRHLLDERGLERVAYL
                     DPDIRLYDRLDEIERLTGEHGLVLIPHITSPLPRDGKRPSEADILISGTYNLGFISLE
                     RSPSTDDLLDWWSERLKTDCVVAPDVGYFVDQRWMDFVHGVMPDFYVLRDPSYNVAYW
                     NLHGRELTYHKGRYYVDGRHLRFFHFSGFDPDHRKRLSKHQTRIEIRRGSALARACNA
                     YADELLAHGYRKAKNWPYSYGVLPNGVTLDSGMHRLYRKGEGAPRQSIFTPEGADRFV
                     AWLNEPAPVGGEHGVTRYLYEIYSGRPDLRQEFPNLDGPDAMRLIEWHRIHGQVKLPY
                     PVHPSAEQDGYETPETPDSPVGVNVAGYFRAELGVGETARQVVEALERTEMPVATVGL
                     SASASRQEHEYETDAVRPTFPVNLICVNADMLPTFADQAGPGFFFDRYTIGLWWWETS
                     EFPERFSRAFEPVDEVWVGSHFVADAISAASPVPVVKMTMPISMPEIEEFDRGDLGLP
                     EGFVFLFVFDYHSVFE"
BASE COUNT          388 a          614 c          694 g          405 t
ORIGIN      
        1 ctcgaagacg ctgtggtaat cgaagacgaa aaggaagacg aagccctcag ggagcccgag
       61 gtccccgcgg tcgaactcct ctatctccgg catggatatc ggcatcgtca tcttcaccac
      121 gggtaccggc gaggcggccg agatcgcgtc ggccacgaaa tgcgagccga cccagacctc
      181 gtccaccggc tcgaaggccc tggagaagcg ttcggggaac tcgctcgtct cccaccacca
      241 caggccgatg gtgtagcggt cgaaaaagaa ccctggtcct gcctgatcag cgaacgtcgg
      301 gagcatgtcg gcgttgacgc agatgaggtt gaccgggaag gtcggcctca ccgcgtcggt
      361 ctcgtactcg tgctcctggc ggctcgccga ggcggagagg ccgaccgtgg ccacgggcat
      421 ttccgtcctc tccagcgcct cgaccacctg ccttgcggtc tcgccgacgc cgagctcggc
      481 gcggaagtac ccggcgacgt tgaccccgac gggcgagtcc ggcgtctcgg gcgtctcgta
      541 gccgtcttgc tctgcggagg ggtgcacggg gtaaggaagc ttaacctgcc cgtggatgcg
      601 gtgccactcg atcaggcgca tcgcgtccgg gccgtcgagg ttggggaact cctgtctgag
      661 gtcggggcgg cccgagtaga tctcgtagag gtagcgcgta acgccgtgct ccccgcctac
      721 gggggccggc tcgtttagcc aggcgacgaa cctgtcggcg ccttctggcg tgaagatgga
      781 ctgtcgcggg gccccctcgc ccttgcggta gagcctgtgc atgcccgagt cgagcgttac
      841 gccgttcggc agcaccccgt aggagtaggg ccagttcttc gcctttctgt acccatgggc
      901 gagcaactcg tccgcgtaag cgttacaagc gcgggccagc gcggagccgc gccggatctc
      961 gatgcgggtc tgatgcttgc tgagccgctt tctgtggtcg ggatcgaagc cggaaaagtg
     1021 gaagaaacgc aggtgtcgtc cgtcgacgta gtagcggcct ttgtggtagg tcagctctct
     1081 gccgtgcagg ttccagtagg ccacgttgta ggaaggatcg cgcaaaacgt agaagtctgg
     1141 cattacgccg tgcacgaaat ccatccaccg ttggtcgacg aagtagccga catcgggggc
     1201 gaccacgcag tccgtcttca ggcgctcgga ccaccagtcg agcagatcgt ccgtacttgg
     1261 gcttcgctcc agggagataa agcccaggtt gtaggtgccc gagatcagga tgtcggcctc
     1321 ggagggcctc ttcccgtcgc ggggtagggg ggaggtgatg tgggggatga ggacgagccc
     1381 gtgctcgccg gtcagcctct ctatctcgtc cagcctgtcg tagaggcgga tatccggatc
     1441 gaggtaagcg acccgctcca gaccgcgctc gtcgagcagg tgccgcagca gccacggctt
     1501 cacggcggtc gagagctcga gcacatcgta gatcgccgcc atgtgctcgt agtcgtcgat
     1561 acctagttgg gccggtgtta ccagctcgaa cggttctaag gaggcgtcta tgtagccttc
     1621 tgtttcgtct atgacgagca cgtggcacgt cccctcgggg tgatgctcct tgtaggactg
     1681 cgccaacacc ctcgcgtggg cgatataatt cttggcgatg atcgtgcaga tattcatagc
     1741 cggcattgaa tcatactcag cctggcactt cgcaaaacgg ccagaaacct gatacggttc
     1801 cgctgttaat cagtgaacct tcaaggtgta aaatgtctgc gtggatgcca ttgaatagag
     1861 cgccgaacaa tttgaagacg caaagtatat ggtccttcac gatctgagac gataccaaga
     1921 ttctggggga gatgtgtgat cgaagcgcga agccgtgcca agtggaagaa cagggaaggg
     1981 atccccgagt acgcgttccg ctggaactgg gtggaggact cctcagactc ggggttcacc
     2041 gcggtcttga gggtcaagga cgaagcccgt tcgctgccct gggtgctgcc tggggtgctt
     2101 c
//
LOCUS       DATMJA010000258         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_686495, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000258 DATMJA010000000
VERSION     DATMJA010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686495"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VNT12_06155"
     CDS             complement(<1..220)
                     /locus_tag="VNT12_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWI45734.1"
                     /translation="MDRERAKEKTETKPVGFWEAFWFWVKLGFINFGGPAGQIAIMHR
                     ELVERKRWVSEGQYLRTLNFCMLLPGPEA"
     gene            complement(341..805)
                     /locus_tag="VNT12_06160"
     CDS             complement(341..805)
                     /locus_tag="VNT12_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWI45735.1"
                     /translation="MQVSAKTDYALRAASELALAATEDAGPIKGDWISETQGIPKKFM
                     ENILHDLKRSGIVRTRRGASGGYWLALPAEEISLADIIRAVEGPLANVRGEWPEAVEY
                     QGAAEHLQEVWIAVRANLRAVLESVTLADIVDGKLPSSITKLTSDPEAWVHH"
     gene            complement(807..965)
                     /locus_tag="VNT12_06165"
     CDS             complement(807..965)
                     /locus_tag="VNT12_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45736.1"
                     /translation="MAKEYLQFAGALVFFLGLLIGSPWFVYVPNQGERKRDMKVEAGG
                     CGLTGSMR"
     gene            complement(1084..1269)
                     /locus_tag="VNT12_06170"
     CDS             complement(1084..1269)
                     /locus_tag="VNT12_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45737.1"
                     /translation="MEEERFEVVAVTLFGKIVVARYATLEQAEWQAGELNEEAERNPR
                     GYVQYLARPAERPAGEH"
     gene            1405..>2408
                     /locus_tag="VNT12_06175"
     CDS             1405..>2408
                     /locus_tag="VNT12_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI45738.1"
                     /translation="MLEGLVQDFQLTLPYFLRRAEQLYRYREIVTRRPDKTFHRHTYA
                     DFVSRSKKLAVALGSLGLEPGDRVGTLGWNTYQHLEAYFGVPSSGLVLHTLNPRLHED
                     DLAYIAGHAEDRVMLVDETLVSVFEKFRDRTGVEHAVVVTEGGDVPEGMISYEDLIGS
                     SEEADFEYPDFDENQAAAMCYTSGTTGRPKGALYSHRSTVLHSMMVAMGNTFALSEAD
                     CVLPVVPMFHVNAWGLPFASVLVGAKLMMPGPNLDAASLLEDLEQEEVTLTAGVPTVF
                     LAVLQALDAEPERYDLSKLRAMVIGGSAAPKGVIKDYKERHGIEVLHAWGMTEMSPIG
                     T"
BASE COUNT          448 a          793 c          692 g          475 t
ORIGIN      
        1 gagcttcagg accgggcaac agcatgcaga agttgagcgt cctcaagtat tggccctcag
       61 agacccaccg tttcctctcg acaagctcgc ggtgcatgat ggcgatctgg ccggcaggcc
      121 ccccgaagtt gatgaaacca agcttcaccc agaaccagaa agcctcccag aagccgaccg
      181 gtttggtctc agtcttctct ttcgcccgct ctctatccac attatctcca cttatcaagt
      241 gaacaatagc gcgagcctaa ccacggccgt ctagaaagtc aaggatcagg cgacagagct
      301 aaaatgctga caagcgcccg acagggcgcg tgccaacaag ctaatgatgc acccaggctt
      361 cggggtcgct ggtgagcttc gtgatcgagg aggggagctt tccgtcgacg atatcggcta
      421 aggttacgga ctcgagtacg gcgcgcaggt tggcgcggac cgcgatccag acctcctgca
      481 ggtgctcggc cgcgccctgg tactctaccg cttcgggcca ctcgcccctg acgtttgcga
      541 ggggtccttc gacggctcgg atgatgtcgg cgagcgagat ctcctccgcc ggcagcgcga
      601 gccagtagcc gcccgaggca ccgcgcctgg tacggactat ccctgagcgt ttcaggtcgt
      661 gaagtatgtt ctccatgaac ttcttgggga tgccctgggt ctccgagatc caatctccct
      721 ttatggggcc cgcgtcctcg gtcgcagcaa gggccagctc ggaggccgcc ctgagcgcat
      781 agtcggtctt ggccgaaacc tgcaagttac ctcatgctcc ctgtaaggcc acagccaccg
      841 gcctcaacct tcatgtccct ctttctctca ccctggttcg gcacgtagac gaaccagggt
      901 gaccctataa gcaaccccag gaagaacacc agcgccccgg cgaactgcag gtactccttc
      961 gccatccaac ctcctctttg ttcggaaccc gtcagacgcc ccgccacatg attttacgat
     1021 agcagcgccg gcttcgccgc ccggtcctgg ttcgagttca ttatgcctcc cgcacgggcc
     1081 ccgttagtgt tccccggccg gcctttccgc tggccgcgcc aggtactgga cgtagccgcg
     1141 cgggttgcgt tcggcctcct cgttcaactc tccagcctgc cactcggcct gctccagcgt
     1201 cgcgtagcga gccaccacta tcttcccgaa gagcgtcacc gccaccacct cgaagcgttc
     1261 ctcttccacc aggctccagt cttgaccccc ttttattctg cctgctaaga tcgtccttag
     1321 caacgctttc cgggagaggg agtataggcg cgctcggggt cgtatctgta gcggaccctg
     1381 cacccgaact atagaggaga cgagatgcta gagggacttg tccaggactt ccaacttacc
     1441 cttccctact tcctgcgccg ggctgagcag ctctacaggt accgtgagat cgtcacccgc
     1501 cgtcccgata agactttcca cagacacacc tacgccgact tcgtctctcg gtccaagaag
     1561 ctcgccgtgg ccctcgggag cctcggtctg gagccagggg acagggtagg cacgctgggc
     1621 tggaacacct accagcacct cgaagcgtac ttcggcgtcc cctcatcggg actcgtgttg
     1681 cacaccttaa atcccaggct gcacgaggac gacctcgcct acatcgccgg ccacgccgaa
     1741 gacagggtca tgctcgtcga cgagaccctg gtgtccgtct tcgagaagtt cagggatcgt
     1801 actggtgtcg aacacgctgt ggtcgtgaca gagggcgggg atgtgccaga aggcatgatc
     1861 tcctacgagg acctgatcgg gtcttccgag gaggccgact tcgagtaccc cgacttcgac
     1921 gagaaccagg cggcggccat gtgctacacc tcggggacga cgggacgccc caagggtgcc
     1981 ctctactctc accgctcgac ggtactccac tccatgatgg tggcgatggg caacacgttc
     2041 gcgctctcgg aggctgactg cgtgctgcct gttgtaccca tgttccacgt caacgcctgg
     2101 ggcctgcctt tcgcctccgt cctcgtcggc gccaagctga tgatgcctgg cccgaatctg
     2161 gacgctgcaa gtcttctcga agacctggag caagaagagg tgacgcttac ggctggtgtc
     2221 ccgaccgtct tcctcgctgt tttgcaggct ctggacgcgg agcctgagag gtacgatctg
     2281 tcgaagctgc gggcgatggt catcgggggg tctgcggcgc ccaagggcgt cataaaagac
     2341 tacaaggagc ggcatgggat cgaggtcctc cacgcctggg gcatgacgga gatgagcccc
     2401 atcgggac
//
LOCUS       DATMJA010000259         3916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_331143, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000259 DATMJA010000000
VERSION     DATMJA010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331143"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VNT12_06180"
     CDS             <1..426
                     /locus_tag="VNT12_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45739.1"
                     /translation="ISFVAALLGIGLAYTLYVARPERAGALAARFSWLHTFLDKGWYF
                     DALYGATFVRFAMWLGRATRDFDRNVIGGLVGGVGRGTLGTGGLLQRLQNGAVQSYAL
                     FILLSVLIIGVIVGAQYAALVVGLIALVTIAAFAVGTRL"
     gene            423..1877
                     /locus_tag="VNT12_06185"
     CDS             423..1877
                     /locus_tag="VNT12_06185"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWI45740.1"
                     /translation="MITTITILIPLLGAILMLLLPDEERLLRYAALGISAVPLVLAVY
                     VYFAFRDDLGVAALSQDVTWIRSLNIGYRVGLGGLGFGMFFLSTFLTVIAVLASRDVQ
                     RNLKQYFAAIFVAEVGMLGVFAAQDLILFYVFFEVTLIPMYLLVGVWGDENRRPAAIK
                     FFIYTFLGSTVMLAGFLAFGILADTYSMNALGGGGGLSRTAQVAVAAPILFGLLVKVP
                     AVPLHSWLLDVYVSSPTSTNVLLSGVLPKLGTYGLIAVAIPLLPQGVEPFLPYVAAFG
                     VINIVYGAFVAFLQPDLKALVAYSSIGTLGFILLGVASGNAVGLNGAVFQQVAHGLYS
                     ALLFILVGVIAASTGTRRIGELGGLAARMPWAGGLLALGALAAMGLPGLAVFVSEFMS
                     IMGVYATFPVQGVLASLGIILSAMYLLYMLARVIFGPIEHPAYEEIGDAGPVEMASVV
                     PLAVLLVVLGVFPALLMSIQRPAVDAVISVIGGS"
     gene            1877..3307
                     /locus_tag="VNT12_06190"
     CDS             1877..3307
                     /locus_tag="VNT12_06190"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWI45741.1"
                     /translation="MTGQAFIALLPELISTGFLILVLMVGVYSDSNAGVAAGLSAIGT
                     LAVFGSAAVLLATGFSGSYFGGGFVVDGFALYFKLIVAGSAFFAVLAAARWSGQTGDA
                     PEYLTLILSVVLGSTLLVSMRDLFGIFLAIELATIPSYAMVAFDRSRRESAEGGMKYL
                     ITGVIASSVLLYGIVLIYGVSGSAMLQDVADTFSGTLTPVAVLGLVLMISGFAFKLSA
                     APFHFWTPDAYQGAPTSAAAFLSVGPKAATFAILLRILLEGMPDAAPTWTAVMAFLAI
                     ITMFVGNLFALRQRNVRRMLAYSSVAHSGYILAAFAAIQGGGVAFAVQAVLIYTAAYA
                     VMNMGAFLVIDLVGEETKSYNGLVRTNPGVAASMTLFMAALVGIPPLSGFFGKLWIII
                     AGAQSGSILVYIVVGALVVNTVLSVPYYFGIIRNMIFEEPTTETARSKGGAAVRFCVY
                     VLAVLTTLFGLLIIPLSALVGASGLA"
     gene            3380..>3916
                     /locus_tag="VNT12_06195"
     CDS             3380..>3916
                     /locus_tag="VNT12_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWI45742.1"
                     /translation="MQAVVLVGGQGTRLRPLTYDIPKSLVPLRNRPFMGYMLDFLRGA
                     GLDGAVLSLGYLPDPIQAYLCDKQDLVGFSVDYAVEDHALGTAGGIKNAEKYLDGDTL
                     VVVNGDVLTGMDLSRTIQVHKASDALATIVLTSVEDPTAYGLVEVDHEMLVHRFIEKP
                     AADEVTTNLVNAGVYVLEP"
BASE COUNT          591 a         1257 c         1200 g          868 t
ORIGIN      
        1 atctcgttcg tcgccgcgct actcgggata gggctcgcct acacactcta tgtcgcgagg
       61 cctgagcggg caggggcgct cgctgccagg ttctcgtggc tccacacgtt cctggacaag
      121 gggtggtact tcgacgctct ctacggtgcg acgttcgtga ggttcgcaat gtggctcggt
      181 agggctaccc gggacttcga ccgtaacgtc atcggtggtc tcgtgggtgg ggtgggacga
      241 ggcactctgg ggacaggcgg gctgcttcag cggctgcaga acggcgcggt ccagagctac
      301 gcgctcttta tcctgctgag cgtcctgatc atcggggtca tagtaggggc gcagtacgcg
      361 gcgttggtag tcggtttaat cgccctggta acgatcgccg ctttcgcagt ggggactcga
      421 ctatgataac tacgataacc atcctcatcc cgctgctcgg cgcgatactg atgctgctct
      481 tgcccgacga ggagcgcctg ttgcgctacg cggccctcgg aatctcggcc gtgcctcttg
      541 tccttgcggt ctacgtctac ttcgccttca gggacgacct cggcgtcgcc gcgctctccc
      601 aggacgtgac ctggatccgg tccctgaaca taggctaccg cgtcggactc ggcgggctcg
      661 ggttcgggat gttcttcctc tcgacgtttc ttacggtcat agcggttctc gcctccaggg
      721 atgtgcaaag aaacctcaag cagtatttcg cagcgatctt cgtcgccgaa gtagggatgc
      781 tgggggtctt cgcggcgcag gatctgatcc tgttctacgt cttcttcgag gtcacgctga
      841 tccccatgta cctgctcgtc ggcgtgtggg gggacgagaa caggcgtccg gccgcgatca
      901 agttcttcat ctatacgttc ctcggctcga cggtgatgct ggccggattc ctggctttcg
      961 gtattctcgc tgatacctac tcgatgaacg ccctcggcgg tggtggtggc ctctcgcgga
     1021 cggcccaagt cgccgtggct gcccccatcc tcttcggatt gctcgtcaag gttccagccg
     1081 tgccgctgca cagctggctt ctggacgtct acgtctccag cccgacttcg accaacgtgc
     1141 tgctctcggg cgttctgccc aagctgggga cctacggcct gatcgcggtg gccatccccc
     1201 tgcttcccca gggcgtcgaa cctttcctcc cttatgtcgc cgctttcggg gtgatcaaca
     1261 tcgtatacgg ggctttcgtg gccttcttgc agcccgatct caaggcgctc gtcgcctact
     1321 cctccattgg aactctgggc ttcatcctgc tcggtgtcgc ctcgggtaac gcggtcggcc
     1381 taaacggggc cgtcttccag caggtcgcac acgggctcta ctcggccctg ctttttatcc
     1441 ttgtcggcgt catagcggcc agcacgggga cccgccgcat aggggagctc ggcgggctgg
     1501 cggccaggat gccgtgggcc ggagggcttc tggccctcgg tgcccttgcc gcaatggggc
     1561 tgccggggct cgccgtcttc gtctccgagt ttatgagcat catgggcgtg tacgcgacgt
     1621 ttcctgtgca gggagtgctc gcgtcgctgg ggatcatact ctcagccatg tacctgctct
     1681 acatgctggc gcgtgtgatc ttcggcccca tcgaacatcc cgcctacgag gagatcgggg
     1741 acgcaggacc ggtcgagatg gcttcggtcg tgccgcttgc agtgttgctc gtcgtgctag
     1801 gcgtcttccc tgccctgctg atgagcatcc aacgcccggc cgtggatgcc gtaatcagcg
     1861 tcataggggg tagctagtga ccggacaggc cttcatcgcg ctcttgcctg agctgatctc
     1921 tacagggttc ctgatcctgg tcctcatggt cggcgtgtac agcgatagca acgcaggggt
     1981 ggcggcaggc ctgtccgcca tagggacgct ggcggtgttc gggagcgccg ccgtcctgct
     2041 cgcgacgggc ttctcgggaa gctacttcgg cggcggcttc gtggtcgatg gcttcgcgct
     2101 gtacttcaag ctgatagtcg caggctcggc cttcttcgcc gtgctcgcgg cggcccgctg
     2161 gtctggccag accggtgacg cgccagagta cctgacgctc atcctctccg tcgtgctagg
     2221 ttcgacgctc ctcgtctcca tgcgggacct cttcggcatc ttcctagcca tagagctggc
     2281 caccatacct tcctacgcca tggtcgcctt cgacaggagc cgccgcgaga gcgcggaggg
     2341 cgggatgaag tacctgatca ccggcgtcat cgcctcctcc gtgctgctct acggcatcgt
     2401 cttgatctac ggcgtctctg gctctgcgat gctccaggac gtggcggaca ctttctccgg
     2461 caccctcact cccgttgcgg tcctcggact cgtgctcatg atctcgggct tcgccttcaa
     2521 gctctcggcg gcgcccttcc atttctggac ccctgacgcc taccagggcg ccccgacgag
     2581 cgcggccgcc ttcctctcgg taggccccaa ggctgcaacc ttcgccatcc tcttgcggat
     2641 tctcctcgaa ggcatgccgg acgccgcacc gacctggacg gcggtgatgg ctttcctggc
     2701 gatcataact atgttcgtcg gcaacctctt cgccctcagg cagcgcaacg tcaggcggat
     2761 gctcgcgtac agttcggtag cccactccgg gtatatcctg gctgccttcg cggcgatcca
     2821 ggggggcggg gtcgcgttcg ccgtgcaggc agtcctgatc tacacggccg cctacgccgt
     2881 tatgaacatg ggcgctttcc ttgtaataga cctggtaggc gaggagacca agagctacaa
     2941 cgggctcgtc aggacgaacc ctggcgtggc ggcgagcatg accctgttca tggccgcgct
     3001 cgtcggcata cccccactct cggggttctt cgggaagctc tggatcatca tcgcaggcgc
     3061 ccagtcgggc tcgatcctcg tctacatcgt ggtcggcgcg ctcgtcgtca acaccgttct
     3121 ctccgtccct tactacttcg gtatcatcag gaacatgata ttcgaggagc cgacaacaga
     3181 aacggcgagg tcgaaagggg gcgccgccgt aaggttctgc gtctacgtgc ttgccgtgct
     3241 taccaccctg ttcggcctgc tcatcatccc tctctccgcg ctggtcggag cctcaggcct
     3301 ggcgtgagga acacagcgca ctacaaagat cgcccacttc gtattggcct gagatccgtg
     3361 cctggtgtaa gctaggtcta tgcaagcagt tgtacttgtt ggagggcagg ggaccaggct
     3421 gcggcctttg acctacgaca ttccaaagtc gctggtgccg ctccgcaacc gtccctttat
     3481 ggggtacatg ctggactttc tgcgcggcgc cggcctcgat ggtgcggtgc tctctttagg
     3541 ttacctgcca gacccgatcc aggcctacct gtgcgacaag caggatctcg ttggtttctc
     3601 cgtagattac gccgtcgagg accacgccct cgggactgcc ggcgggatca agaacgccga
     3661 aaaatacctc gacggtgaca cgctggtcgt cgtcaacggc gacgtgctca cgggtatgga
     3721 cctctccaga acgatacagg tgcacaaagc gtcggacgcc ctggccacca tagtcctgac
     3781 gagcgtagag gatcccacgg catacggcct cgtcgaggtc gaccacgaga tgctggtaca
     3841 ccgctttatc gagaagccgg ctgccgatga agtgacgacg aacctggtca acgccggtgt
     3901 ctacgtgctc gagcct
//
LOCUS       DATMJA010000260         3167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_746200, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000260 DATMJA010000000
VERSION     DATMJA010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746200"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VNT12_06200"
     CDS             complement(<1..570)
                     /locus_tag="VNT12_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWI45743.1"
                     /translation="MLARDERANLGKQSSKGRLGAGITRRRFLGALTAGTALVALSGS
                     LGCEPSARTRASAAPQGPGQARTFRSRPDLRPPAIEVNTNLPGTAPGHIFVSPKKGPG
                     EEAPTQDAPLIVDSSGEPVWFHPLQDAEADAFTFEVQKYKGETVLTWWEGLHTGYGQG
                     EYVIFDHSYREIKRFGAGNGYEGDHHEFLI"
     gene            complement(924..1724)
                     /gene="mltG"
                     /locus_tag="VNT12_06205"
     CDS             complement(924..1724)
                     /gene="mltG"
                     /locus_tag="VNT12_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWI45744.1"
                     /translation="MQARYEGYGAEIKTGRYTFQPGWDSGRILHKLTVGQAAPTITVT
                     IPEGLTLEETAGTVAADSSVPVRAFEEAARRTDYGYAFLDNPDVKTTEGYLFPAKYDF
                     EKDVTARQIVDRLLGQYLLETQDLDIADAKERLDLSENQVVTVASLIEKEAASPEEKS
                     LIASVIYNRMRKDTPLQIDATIQYAIKRPKASLSLADLKVDSPYNTYENKGLPPGPIC
                     SPGTESLLAALNPTNTNYLYYVLEANGEKHFFTKSYDDFLRAKAKAER"
     gene            complement(1725..2039)
                     /locus_tag="VNT12_06210"
     CDS             complement(1725..2039)
                     /locus_tag="VNT12_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45745.1"
                     /translation="MRRPDKSNGEDREELDARLDALQQSDSRRVQRKRRANAESAVLG
                     VLLVLGVLGAIFVIYSAATGGENEPAPGESARVQVIKGDTLSDVAAKLEAAGIIESAF
                     VF"
     gene            complement(2023..2445)
                     /gene="ruvX"
                     /locus_tag="VNT12_06215"
     CDS             complement(2023..2445)
                     /gene="ruvX"
                     /locus_tag="VNT12_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWI45746.1"
                     /translation="MSPADRGRVAALDLGEARTGVAVSDPGGVIASPLEVIPSGDLSN
                     YLSRLVDEEGVYEVVVGVPRTLGGEVGFQARKVLSRLDALKKEFPGVRFVEWDERFTT
                     RLAGSRGRKRGTERVDHLAAARMLQEYLGARGAFEAPR"
     gene            complement(2449..>3167)
                     /locus_tag="VNT12_06220"
     CDS             complement(2449..>3167)
                     /locus_tag="VNT12_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HWI45747.1"
                     /translation="MLFGEKYADLVRVVEIDGFSRELCGGTHVRGTAEIGAFKILSNR
                     KHGADLYRIEVITGREALYYLTGATEKAEEISEALKVEIEGLPGAVGRLREEVQSSRE
                     AAREQALKKGLEEVGDLVEGAQQVNGTRVVTGQVVAADVRGLRQISDDVKNRLGGPSA
                     VVLAAALGGKAVLVANLHPEVSRRVEAGEIVREVSGVLGGGGGGGATMAQAGGGNLEA
                     IPDALARVREILDQRLADPE"
BASE COUNT          555 a         1036 c          926 g          650 t
ORIGIN      
        1 gatgaggaac tcgtgatggt ccccctcgta gccgttgccg gccccgaacc tctttatctc
       61 cctgtaggag tggtcgaaga tcacgtactc gccctggccg tatcctgtat ggagtccctc
      121 ccaccaggtg agcaccgtct ctcctttgta tttctggacc tcgaaggtga aagcatcggc
      181 ctcggcatcc tgcagcggat ggaaccagac aggctcaccg ctggagtcga ctattagcgg
      241 tgcgtcctgg gtgggagctt cctctcctgg accctttttg ggcgagacga agatgtggcc
      301 aggggccgtg ccaggcagat tcgtgttcac ttcgatggcg ggcggacgga gatccgggcg
      361 tgagcggaaa gtcctcgcct gtccaggtcc ctggggcgcg gcggacgccc gggtgcgcgc
      421 gctcggctcg caaccaaggg agccggaaag tgcgaccaag gcggttccgg ccgtgagggc
      481 cccgagaaac ctccggcgtg tgatccctgc tccaagtctc cctttgctcg actgcttgcc
      541 caaattcgcc ctctcgtctc tcgccaacat cgaaagggag taggcgccag gcctgctccc
      601 ctacacacga gcggaagcca cgaagacctc cggtcccacg ccacatgtgc tccgtcaagg
      661 agtttcggcg ggctcgaacg cctcacgagt aaacacactc ctgttcggat cgaccctgta
      721 aagagggatc ttccacgaaa aaccgcagat ttgcaaggcc tgtcagatag caggcacggc
      781 ggcttgtcag ccaggacttt caggcacacc agctaaaatc tgacaagtcg cctgttcccc
      841 aggaactgtg ctgacaagcg aagcccgaag ggcatagcgt gctgaaatgc ttgtaaagag
      901 gtacaacctc tttgcaaacc tccctagcgt tctgccttcg ccttcgcccg gaggaaatca
      961 tcgtagctct tggtaaagaa gtgcttctca ccgttggctt caaggacgta gtagaggtaa
     1021 ttggtattcg tggggttcag cgcggccagg agtgactccg ttcccgggct gcatatgggg
     1081 cctggcggca atcctttgtt ctcgtaggta ttgtaaggtg aatctacctt caggtcggcc
     1141 agcgagagac tcgccttggg tctcttgatt gcgtactgga tcgtggcgtc gatctggagc
     1201 ggcgtgtcct tgcgcatgcg gttgtagatc accgacgcta tcagcgactt ctcctcgggg
     1261 ctagcggcct ctttctcgat cagggaggcc acggtcacaa cctgattctc cgaaaggtcg
     1321 agacgctctt tcgcgtcagc gatgtcgagg tcctgggtct cgagtagata ctgcccgagg
     1381 agcctgtcga ctatctgcct ggcggtaacg tccttctcga aatcgtactt ggcaggaaag
     1441 aggtaccctt ctgttgtctt gacgtcaggg ttgtcgagaa aagcgtatcc gtagtcggtt
     1501 ctacgcgcag cctcctcgaa cgcgcgcaca gggacactgc tgtccgcggc gacggtccct
     1561 gcggtctcct ctaaggtgag tccctcgggg atcgtgaccg tgatcgtagg agcggcctgt
     1621 ccaacggtca gtttatggag aatcctcccg ctatcccatc cgggctggaa ggtataacgt
     1681 ccggtcttta tctccgcacc atacccttca tacctggcct gcagctagaa gacgaaggcg
     1741 ctctctatga tcccggccgc ttcgagcttg gcggcgacgt ccgagagcgt atcgcctttg
     1801 atgacctgga ccctcgccga ttccccaggc gcaggctcgt tctcgccacc tgttgccgcg
     1861 gagtaaatga cgaagatggc ccccagaacg ccgagcacga gaaggacgcc tagcacggcc
     1921 gactcggcgt tcgcgcggcg tttgcgctgg acccttctgg agtcggattg ctggagggcg
     1981 tcgagcctgg catcgagctc ctcacggtct tctccattgc tcttatcggg gcgcctcaaa
     2041 ggcccccctc gccccgaggt actcttgcag catccgtgcg gccgccaggt gatccaccct
     2101 ctcagtcccc ctctttctgc cgcggctacc ggctagacgg gtcgtgaatc gctcgtccca
     2161 ctccacgaac cgcacgccag ggaactcttt tttcaaggcg tctagtctag acaaaacttt
     2221 gcgcgcctgg aagccgacct ccccgccgag cgttctaggc accccgacga cgacctcgta
     2281 gacgccctcc tcgtccacga gccggctcag gtaattggag agatccccgc tcggtatcac
     2341 ttcgagtggg ctggcgatca cgcctccggg gtccgagacg gcaacgccgg tgcgcgcctc
     2401 tccgaggtcg agggcggcga ctcgcccgcg gtcggctggc gacaggactt actccggatc
     2461 cgcgagacgt tggtcgagga tctcgcgtac cctcgctagg gcgtcgggga tagcctcgag
     2521 gtttccgccg ccggcttggg ccatcgtcgc gccgccaccc ccgcctcctc ccagtacgcc
     2581 cgagacctcg cgcactatct ccccggcctc gaccctacgg gagacctcag ggtgtaggtt
     2641 ggcgacgaga acagccttgc ctccgagcgc agcggcaagc accacggcag aaggaccgcc
     2701 tagacgattc ttgacgtcgt cggagatctg gcgcaacccc ctgacgtcgg cggcgacgac
     2761 ctggcccgtt accaccctcg ttccgttgac ctgctgggcg ccttcaacga ggtcgcccac
     2821 ctcctcgaga cccttcttta acgcctgctc cctggccgcc tcacgagatg actgtacctc
     2881 ctcgcggagc cgccctacgg ccccagggag gccctcgatc tcgaccttca gggcctccga
     2941 gatctcttct gccttctcgg tcgccccggt aaggtagtag agtgcctccc tacctgttat
     3001 gacctcgata cggtagaggt cggcgccgtg tttcctgttg gagagtatct tgaaagcccc
     3061 gatctcggcg gtgccacgca cgtgcgtccc gccgcacagc tcgcgcgaga atccatcgat
     3121 ctccactacg cgtaccaggt cagcgtactt ctccccgaag agcatca
//
LOCUS       DATMJA010000261         3961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_153989, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000261 DATMJA010000000
VERSION     DATMJA010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153989"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VNT12_06225"
     CDS             <1..793
                     /locus_tag="VNT12_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWI45748.1"
                     /translation="PVRLGNGAYKQRQLDLYGAVLDAAYLYNKHGAPLDYDLWKNLGK
                     ILDWLSENWREPDEGIWEVRGGRRQFVSSKLLSWVALERAGRIARQRGLPSGDGRWKA
                     ERDAIYEEIIEKGWNPEKNSFVQYYGSDALDASLLMMPLVKFVGPMDPRWISTLDRIQ
                     QELAYDTLVKRYRTECAAPDGLEGGEGNFSLCSFWLVECLTQAGRLDEARLALEKMFS
                     YANHLGLYAEELGHSGEALGNFPQALTHLALISAAVHLDRALGAR"
     gene            800..1711
                     /gene="ftcD"
                     /locus_tag="VNT12_06230"
     CDS             800..1711
                     /gene="ftcD"
                     /locus_tag="VNT12_06230"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HWI45749.1"
                     /translation="MRPPLFEAVANFSEGKDATKISRITEAVRAISGVRILGLHSDPD
                     HNRSVLTFAGGVDSVLEGAIALAKACAAELDLASQSGVHPRMGVLDVLPFVPLEGANM
                     DDAVRLAHRAGESLGSLGFPIYLYGSAATTKHRRSLPDVRRGGYEGVASRLEDPLWKP
                     DYGPKTLDPHRGAVAVGARPFLVAFNAYLDTDDVEVARAVARKVRESGGGLPAVRALG
                     FGVGGKAQVSMNLTDLEQTPIPVALEAVRSAASGFGASVESTELVGLAPLEAVLQTAR
                     YYLGLRELDAHHVLEWTLYGQTDSRSL"
     gene            1821..2645
                     /locus_tag="VNT12_06235"
     CDS             1821..2645
                     /locus_tag="VNT12_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWI45750.1"
                     /translation="MNGRKITAAAIQMSSTPNKGENLDTAERLIRSAASSGADLIALP
                     ELWNCHGLEDVYRENAEPVPGPTTEFLGGLANELGVYLLGGSILEGDPQSTKLHNTST
                     FFGPDGRMSAVYRKIHLFDVKAPDREYLESRTIAAGTKIATAKAGAATVGLSVCYDVR
                     FPELYRLLALRGAEVLAVPAAFTLQTGKDHWELLLRARAVENQAYVVAPAQWGRKADG
                     RWTYGRSIVVDPWGTVLAQCPDRDGYALATLDLDYLDRFREEFPALKNRRPETYDW"
     gene            3047..3619
                     /locus_tag="VNT12_06240"
     CDS             3047..3619
                     /locus_tag="VNT12_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI45751.1"
                     /translation="MFGPVVRAVEDEELIHRVAEGDDLALSELYDRYSRPVYATGIRV
                     LGDASLAEELVQDAFMSVWRAAGSFDPGRASFTTWLYRITRNRAVDLNRRRQVRPFSA
                     GEESLRNVSGGPEPEGGVDGWDVARALSRIPDEHREVLTLAYFEGLSQREISRRTGVP
                     LGTVKSRTTAALKRLHRSLVNPAAEESRHD"
     gene            3612..>3961
                     /locus_tag="VNT12_06245"
     CDS             3612..>3961
                     /locus_tag="VNT12_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWI45752.1"
                     /translation="MTEDRFEKLLGLYLLGELSVEEERELERHLEECSGCRGELARAR
                     QTHDLLRELAAAGPPPELKDRVLARVRGEIPARSRGGWRLWVPAAALLVISVLGAGLL
                     WTITKDSSEGIPLAA"
BASE COUNT          704 a         1257 c         1300 g          700 t
ORIGIN      
        1 ccctgtaagg ctcggcaacg gcgcctacaa acagcgccag ctcgacctct acggggccgt
       61 gctcgacgca gcttacctct acaacaagca cggcgccccc ctcgactacg atctctggaa
      121 gaacctcggc aagatactgg actggctgag cgagaactgg cgggagcccg acgagggcat
      181 ctgggaggtc agagggggca ggcgccagtt cgtctcttct aagctcctca gctgggtggc
      241 cctggagcgg gcgggcagga tcgcccgcca acgcggactg ccttcggggg acgggcgctg
      301 gaaggccgag cgcgacgcca tctacgagga gatcatagag aagggctgga accccgagaa
      361 gaacagcttt gtacagtatt acggctcgga cgccctcgac gcctccctgc tcatgatgcc
      421 gctggtcaag ttcgtcggcc caatggaccc acgttggatc tccaccctcg accgtatcca
      481 acaggaactg gcctacgaca cgctcgtgaa gcgttaccgg actgagtgcg cggctcctga
      541 cggcctcgaa gggggcgagg gcaacttcag cctgtgctct ttctggctcg tcgagtgcct
      601 cacgcaggcg ggacgactcg atgaagcgcg cctggccctg gagaagatgt tctcctacgc
      661 caaccatctg ggtctctacg ccgaggaact cgggcactcc ggcgaggccc tcggcaactt
      721 cccccaggcc ctcacccacc tggcgctcat atccgccgcg gtccacctgg atcgcgcgct
      781 cggagcacgc tgaaccgccg tgcgccctcc gcttttcgag gccgtcgcta acttcagcga
      841 ggggaaggac gccacgaaga tctcacgcat cacagaggcc gtccgcgcca tctcaggcgt
      901 ccgtatcctc ggcctccact ccgaccccga ccacaacagg agcgtcctca cgttcgccgg
      961 cggagtagac tctgtgctcg agggcgccat cgccctggcg aaggcttgcg ccgccgagct
     1021 cgatctcgcc tcgcagtccg gcgtgcaccc taggatgggc gtgctcgacg tgctcccttt
     1081 cgtgcccctc gaaggcgcca acatggatga tgccgtccgg ctggcacacc gggccggcga
     1141 gagcctgggt tcccttggat ttcctatcta cctctacggg tctgccgcga cgaccaagca
     1201 ccgcagatct cttcccgacg tcagacgggg tggctatgag ggggtcgcat cccggctcga
     1261 ggatcccttg tggaagccgg attatggtcc gaaaacactc gatccccaca gaggagccgt
     1321 cgccgtcggc gcccgtcctt tcctggtcgc tttcaacgcg tacctggata cagacgacgt
     1381 cgaggtagcg cgagccgtgg ccagaaaggt cagggagagt ggcggcggcc ttccggccgt
     1441 gagggcgctc ggcttcggtg tcggcggcaa ggctcaggtc tcgatgaact tgaccgacct
     1501 cgaacagacc cctatccctg tggccctgga agctgttcgt tcggctgcgt ccggcttcgg
     1561 cgcttctgta gagtcgaccg agctcgttgg cctggcgccg ctggaggcag tgctgcagac
     1621 ggcccgctac taccttggct tgcgggaact ggatgcccac catgtcctgg agtggacgct
     1681 ctatggccag actgattcta ggagcttgta gtggcatgct gatctacctc gcaggacaag
     1741 gatccggcgt cgaagcgggt attgaatgac ccgggtagta gtctacgtag cgcacttccg
     1801 gcacagaaag gaatggtgtg atgaacgggc gtaagatcac tgcggcggcc atacagatgt
     1861 cctcgacccc gaacaaaggg gagaacctcg acacggcgga acggctcata cgttcggccg
     1921 catcctcggg ggccgacctg atagccctgc ccgagctgtg gaactgccac ggcttggaag
     1981 acgtctacag agagaacgcc gagcccgtac cggggccgac gacagagttc ctcggcggcc
     2041 tcgcgaatga gctcggagtg tatctgctcg ggggttcgat cctcgaagga gatccccaat
     2101 ccacgaagtt gcataatacg tccactttct tcgggcctga cggtaggatg tcggccgttt
     2161 accgcaagat acacctcttc gatgtgaagg ccccggatcg cgaatacctg gagagcagga
     2221 ctatcgcggc cggcacgaag atagcaactg cgaaggctgg cgccgcgacc gtcgggctct
     2281 ctgtctgcta cgacgtgcgc ttccccgagc tctaccgtct gctcgcgctg cgcggcgccg
     2341 aggtactcgc tgtccctgcc gccttcacgc tccagacggg caaggaccac tgggagcttc
     2401 tgttgcgggc gcgggccgtg gagaaccagg cctatgtcgt cgctccggcc cagtggggga
     2461 ggaaagccga cggacgatgg acctacggac ggtccattgt cgtcgacccg tggggtacgg
     2521 tgctcgccca gtgccccgac cgcgacggat acgcgctcgc caccctggac ctcgactacc
     2581 tcgaccgctt tcgggaagaa ttccccgccc tgaagaatcg ccgccccgaa acctacgact
     2641 ggtaggagag tcaaccccta agtgggcgag ctgcggcgct ggcggtagcg tctggaacgc
     2701 acccgcgcgc cgcactggga gccgcaccag gcggccgaat ggttcttggt gtggtcgtag
     2761 agtgcccggt ggcactcggc gcaaaccttg aggcgggacc acgtcccatc ctgctgcgcc
     2821 gcgaatacga tcgcgactgc aacaaaccat cgggctgcat cgttgcgtac aaaccattat
     2881 agggattggc gaggacgctg tggagtgtcc tacatcctgt cttggatgca ggccgatgag
     2941 cggtccggtc gaaccctaca ggttttactg tggtcgcaga ggcggcccca ggaaatgccc
     3001 gagtggggtt tgaactgcta gcccgggact cgcgggtggg agggagatgt ttggaccggt
     3061 agtgagggct gtggaggacg aggagttgat acaccgggta gcggaagggg acgacctggc
     3121 gctctcggag ctctacgatc gttactcgcg gccggtatac gccacgggaa tcagggtgct
     3181 tggggatgcc tccctggctg aggagttggt ccaggatgcc ttcatgagtg tctggcgggc
     3241 ggcgggatct ttcgaccctg gtcgggcgag tttcacgacc tggctctaca ggatcacccg
     3301 caaccgggct gtggatctca atcgcaggcg gcaggtacgg cccttctctg ccggcgagga
     3361 gtcgctgcga aacgtgtcag gcggccctga gcctgaggga ggggtcgacg gttgggacgt
     3421 cgccagggcg ctctcgcgca tccccgatga gcacagggaa gtattgaccc ttgcgtactt
     3481 cgaaggactc tcacagcgcg aaatctcccg ccgtaccggc gtccctttgg ggacggtcaa
     3541 gagccggacc acggccgccc tcaagaggct ccaccggagc ctggtcaacc ccgcagcgga
     3601 ggagtctcga catgactgaa gaccgcttcg agaagctcct cggcctctac cttctcgggg
     3661 aactatccgt ggaggaggag cgtgagctgg agcgtcatct agaggagtgt tccggatgca
     3721 ggggtgagct ggctcgcgcc cgccagacgc acgacctcct ccgagagttg gccgccgccg
     3781 ggccgccgcc ggagctcaag gaccgtgtgc tggcgcgggt gaggggcgag atccctgctc
     3841 gctcccgagg cggatggagg ctctgggttc cggcggccgc gctgctggtc atcagtgtcc
     3901 tcggcgccgg tctcctatgg acgatcacca aagactcctc ggaaggcatt ccgctggccg
     3961 c
//
LOCUS       DATMJA010000262         2463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_864542, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000262 DATMJA010000000
VERSION     DATMJA010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864542"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1139
                     /locus_tag="VNT12_06250"
     CDS             111..1139
                     /locus_tag="VNT12_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWI45753.1"
                     /translation="MVRVVQAGLGIWGRSWAGVVAGAPGVELAAVVDPDPEARRIVEA
                     TPGYGVLEEALQAVECDAVLVASHPGTHHEVAEAALEAGKHVLCEKPLATTLQDAFDL
                     VETADKAERVLMVSQNYRYNAPFRAVQRLLMEGELGELASIRISCRRDTRTLFARDDF
                     RYSMRHPYVLDMSIHHFDLIGAATGRDVRGVYARSWRVPDSPFVHHPAVAAILDLDDG
                     VPVIYQGDWATHEPETSWNGDWEILGEAGRLLWSGSNEDRGTGDVVFERWGRESRLVE
                     QRDLEFVEREATLQALRAAIEGGEPPETAAADNVRSLAAMLGCVTSIESDEPVDVAAL
                     LVARGAKL"
     gene            1190..2191
                     /locus_tag="VNT12_06255"
     CDS             1190..2191
                     /locus_tag="VNT12_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWI45754.1"
                     /translation="MARPVTLFTGQWADLTLEELASKCGEWGFDGLELACWGDHFDVD
                     KALSESGYLEGRWEILRSNGLNCWAIGTHLVGQAVCDPIDARHRATLPPDVWGDGDPE
                     GVRRRAAQKVMDTARAAARFGVTQVNGFTGSSIWHLLYSFPPNDFAEIERGYEDFAKR
                     WNPIIDVFDEEGVRWGLEVHPTEIAYDFVTTRKALDAIGNREGFGINFDPSHFAHQFL
                     DSAAFIEEFADRIYHFHIKDSRKRLNGRRSILGGHLDFGSPERGWDFVSPGHGDVNLE
                     DCIRALNRIGYEGPLSIEWEDSGMDREYGARDALAFARRTDFEPSTQAFDAAFAREE"
     gene            2194..>2463
                     /locus_tag="VNT12_06260"
     CDS             2194..>2463
                     /locus_tag="VNT12_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWI45755.1"
                     /translation="MERGEIGVGLVGYKFMGRAHSNAYRQVASFFDVDPSPRMAAICG
                     RDETGVKEAADTLGWDSYETDYRKLIARDDIDLVDIVTPGNTHREI"
BASE COUNT          468 a          752 c          842 g          401 t
ORIGIN      
        1 ggaggtcaca ccttgaacta cggacgggtg cctggcgtag agaagccggt agcgcggatc
       61 gtgcagggca cagtaatgat cagatccgaa gaagggcagc aggagcggaa atcgtccgcg
      121 tagtccaggc aggactcggt atctggggcc gtagctgggc cggggtagtg gcaggagccc
      181 ccggcgtaga gctcgccgct gtcgtcgatc ccgaccccga agctcgccgt attgtagaag
      241 ccaccccagg ctacggagtc ctggaggagg cgctccaggc cgtcgagtgc gacgccgtgc
      301 tggtcgcttc gcatccgggc acgcaccacg aggtggcgga ggccgcgctc gaggctggca
      361 agcacgtcct ttgtgagaag cctctggcta ccaccctcca ggacgccttc gacctggtcg
      421 agaccgcaga taaagcggag cgtgtcctta tggtcagcca gaactaccgt tacaacgctc
      481 cgttccgcgc ggtgcaacgg ctcctgatgg aaggcgagct cggggagctc gcgtcgatca
      541 ggatttcctg caggcgagac acgcggacgc tcttcgcgag ggacgacttc aggtactcca
      601 tgcgccaccc ttacgtgctc gatatgtcca tccaccactt cgacctcata ggggcggcga
      661 ccgggcgtga cgtgcgcggt gtttacgccc gtagctggcg cgttcccgac agcccgttcg
      721 tccaccaccc tgcagtagcc gcgatcctgg atctcgacga cggagtgccc gtcatctacc
      781 agggagactg ggcgacccac gagcccgaga cctcatggaa cggagactgg gagatcctcg
      841 gtgaggccgg acggctactc tggagcggca gcaatgagga ccgcgggacc ggggacgtgg
      901 tcttcgaacg gtggggccgg gagtcgcgtc tcgtcgagca acgcgacctc gagttcgtgg
      961 agagggaggc gacgctacag gcgctgcgcg cggcgatcga gggcggcgag ccgccggaga
     1021 ccgctgccgc agacaacgtg aggagcctgg ccgccatgct tggttgcgta acctctatcg
     1081 agagcgacga gcctgttgac gtggccgcgc ttctggtcgc gaggggcgct aagctatagg
     1141 ggcgggtcgg ggccggtccg cgaggtagag agtaagagga ggttgcaacg tggccagacc
     1201 ggtaacgctc tttacgggac agtgggctga cctgacgctg gaagagctcg ccagcaagtg
     1261 cggggagtgg ggtttcgacg ggctcgagct cgcatgctgg ggggaccatt tcgacgtaga
     1321 caaagctctc tcggagagcg gctacttaga gggacgctgg gagatactgc gcagcaacgg
     1381 cctgaattgc tgggccatag gaactcacct ggtcggccag gctgtctgcg accccataga
     1441 cgcccgccac cgggcgacgc tccctcccga cgtatggggc gacggcgacc ctgaaggcgt
     1501 gcgccgccgt gccgcccaga aggtcatgga cacggccagg gccgcggccc gcttcggcgt
     1561 gacgcaggtc aacggcttta cggggtcgag catctggcac ctgctctact ctttcccgcc
     1621 caacgacttc gcggagatag aacgcggcta cgaggacttc gccaagcggt ggaacccgat
     1681 catcgacgtc ttcgacgagg agggcgtcag gtggggcctc gaggttcacc cgacggagat
     1741 cgcctacgac ttcgtgacca cacgtaaagc tctcgacgcc atcggcaacc gcgaggggtt
     1801 cggtatcaac ttcgatccaa gccacttcgc ccaccagttc ctcgactccg cagcgttcat
     1861 cgaggagttc gcggatagga tctaccactt ccacatcaag gacagccgca agaggttgaa
     1921 cggtcgcagg agcattctcg gaggccacct ggacttcggc agccccgagc gcggctggga
     1981 cttcgtctcc ccaggccacg gcgacgtgaa cctcgaggac tgcataagag cgctgaaccg
     2041 tatcggctac gagggtcctc tcagtatcga gtgggaggac tcggggatgg accgcgagta
     2101 cggggccagg gatgcgctgg ctttcgctag acgtacagat ttcgagccgt cgacacaggc
     2161 cttcgacgca gccttcgcga gggaggagta atcatggagc ggggagagat cggggtcggg
     2221 ctcgtcggct acaagttcat gggccgcgcc cactccaatg cctaccgtca ggtcgcaagc
     2281 ttcttcgacg tcgacccatc tcccaggatg gccgcgatct gcggccgcga cgagaccggc
     2341 gtgaaggagg cggcggacac tttgggctgg gactcctacg agactgacta ccgcaagctc
     2401 atagcgcgcg atgatataga cctcgtggac atcgtcacgc cgggcaacac gcaccgcgag
     2461 atc
//
LOCUS       DATMJA010000263         4396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_272438, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000263 DATMJA010000000
VERSION     DATMJA010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272438"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VNT12_06265"
     CDS             <1..414
                     /locus_tag="VNT12_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWI45756.1"
                     /translation="FWGVKFKPGKPLFYGTRGETRLFGLPGNPVSAVVCFDLFVRPAL
                     MGMMGRGDRSRPRVPVYFERDVVNKVGRLHAMRVSLKRTEKGWLAESVGAQGSGLVSS
                     LTRADALALVGPASEGIPAGEPVEAIVLRQEKLVG"
     gene            541..1596
                     /locus_tag="VNT12_06270"
     CDS             541..1596
                     /locus_tag="VNT12_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWI45757.1"
                     /translation="MFESDLEDNVFRRRLMKEVAPGVPVHFSFYSPSVKVQVMHPAEN
                     LPGANGRYIGDAVTIEFVPDDAAAVERVREYVWHWEDYNAFTAVRKINHLRRGVCPED
                     VQGILERVRGIDKRAAAKTAEVLDPEELGELLSRGDFKTLARLPGMGEKRARAAVEFY
                     ENERGDRRFLYAANRNDRFRGKPVITELDLGGDLEAQVWEHAVLARRLGDPDPLGPEE
                     SPGHTTVRNANLMHPLPMTPSLMGRGVYYPEQIGLFADTLRRVLIDGEKLAGVGSLRI
                     QRGKLFHTTKMAGVGMKTRARVLASGLLDDEYTYESLVAIPGVTEAQATTIASAARAV
                     KAGPVPVGAARTEVRVY"
     gene            1586..3385
                     /locus_tag="VNT12_06275"
     CDS             1586..3385
                     /locus_tag="VNT12_06275"
                     /inference="COORDINATES: protein motif:HMM:NF005906.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HWI45758.1"
                     /translation="MSTSLYGFVKDHEPVSPEEISTEFLSLTSPNGDARALVERLVRG
                     DSRFIWEGEGLRTVHATSLALGEAPYVVFDVETTGSAAGKGGAITEFGALKLVRGEVV
                     DQFSTLVNPGRPIDPFVVRLTGITDRMVSDAPSISEVLPRFEEFVEGCVLVGHNVHFD
                     CSFVTAARGGTPLPNEVLDTLKLARCLVPGLKRYRLSSLVSHFGVRQAPNHRALSDAA
                     ATTEVFRKLLKLLGSAGIKSVGEALTIRGGRGRIKPQKQHLASGMPNTPGVYYFLDKH
                     GTTLYVGKAKDLKARVRTYFNGGDGRRKVGRLVEEVAEVRVRETESELHALVLEAREI
                     KRLLPRYNSVGRDDKASWFIRLDLGDSYPVPERVSENPPEGALIHLGPYRSAGILDTC
                     IEALGRIFPLKRCSGDGEVCFYGQMGRCAPCIGMDEHEYRERVIDELVALLRGEGGEE
                     HLRALVRERERLAAGLEFEAAARLRDLISGIERIRFSRTVVDAEGVQAVVAPSTEPGV
                     VEVFILCAGRLIAHGGFEPGDASGLGRFADEVLARPQENGSSPKKEGSNEARIVAAYL
                     RRRQAVVEAVRLKEARDLLKAAKRVAEAAAPLA"
     gene            3503..4180
                     /locus_tag="VNT12_06280"
     CDS             3503..4180
                     /locus_tag="VNT12_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI45759.1"
                     /translation="MTRKILLVDDEPSLQKLLTHALEREGFQVQVAGDGEAALEAFKT
                     YEPHLIVLDIMLPKLDGTEVIRRIRAQSDVPVIMLTARDDEIDRVVGLELGADDYVTK
                     PFAVRELVARVRAIMRRAAIPAGQRPDELTYDRLKINLPSRRVSADGEEVDLTYTEFE
                     LLVTLASSPGRVFSRSALLRRVWGDEFRDERTVDVHIRHLREKIERDPRNPEFIHTAR
                     GVGYVFR"
     gene            4167..>4396
                     /locus_tag="VNT12_06285"
     CDS             4167..>4396
                     /locus_tag="VNT12_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45760.1"
                     /translation="MFSAKERSSPDLDFRRKASKLLAPKTGGEFRPRLSMRIWLTALF
                     VLVTAFAAITAYEIVHPILYETLNRSSEAAFK"
BASE COUNT          826 a         1344 c         1469 g          757 t
ORIGIN      
        1 ttctggggcg tcaagttcaa gcctggcaag cccctgttct acggtacgcg cggcgagacc
       61 cgcctcttcg gccttccagg caacccggtc tccgcggtgg tctgcttcga cctcttcgtc
      121 aggccggcct tgatgggtat gatgggccgc ggggacagga gtaggccgcg cgtcccggtc
      181 tacttcgaga gggacgtcgt caacaaggtc ggcaggctgc acgctatgcg cgtcagcctg
      241 aagaggacgg agaaaggctg gttggccgag tcagtcgggg cacagggctc ggggctggtc
      301 agctcgctga cgagagccga cgccctcgcc ctcgtcggcc cggcctcaga gggcattccc
      361 gccggagagc ccgtcgaggc catagtgctg cggcaggaaa agcttgtcgg ctagtcagca
      421 cctcagcatt cgcacaagca agacctgcct gaaatgctga aagcgagggc cgaaggcccg
      481 aagcgtgctg acaggcggag gcgaagccgg agcgtgctga catgctagaa tcagggtccg
      541 atgttcgagt cagatctcga ggataacgtc ttccgcaggc ggttgatgaa ggaggttgca
      601 ccgggggttc cggtgcactt ctctttttat tcgccttcgg tcaaggtaca ggtgatgcac
      661 cccgcggaga atctcccggg ggccaacggc cgctacatcg gggacgccgt caccatcgag
      721 ttcgtgcccg acgacgctgc ggccgtcgag cgggtccgcg agtacgtctg gcactgggag
      781 gactacaacg ccttcacggc ggtgcgcaag atcaaccacc tcagacgcgg tgtctgccct
      841 gaggacgtgc agggcatcct ggaaagggtc cgcggcatag acaagcgggc ggccgcgaag
      901 acggccgagg ttttggatcc tgaagagttg ggggagttac tctccagggg ggacttcaag
      961 acgctggcgc ggctgccagg catgggtgag aaacgggccc gtgccgccgt cgagttctac
     1021 gaaaacgaga ggggcgaccg gcgctttttg tatgccgcca accgcaacga ccgtttccgg
     1081 ggcaagccgg tgatcacgga gctggacctc ggcggagacc tcgaggcgca ggtgtgggaa
     1141 cacgccgtgc tggcgcgaag gctgggtgac cccgacccac tcgggcccga ggagtctcct
     1201 ggccacacca ccgttcgcaa cgcgaacctc atgcacccgc tcccgatgac gccttcattg
     1261 atggggcggg gcgtctacta ccccgagcag attggactct tcgcggatac tttgagacgt
     1321 gtcctcatag acggggagaa gctggccggt gtcggatccc tacgcatcca gcgcggcaag
     1381 ctcttccaca ccaccaagat ggccggcgtc gggatgaaga cgcgggccag ggttcttgcg
     1441 agcgggttgc tcgacgacga gtacacctac gagagtctcg tcgccatccc aggtgtcacg
     1501 gaagcacagg ccacgaccat cgccagcgcc gcaagggccg tgaaagcggg gcctgtccct
     1561 gtaggagcgg cgcggacgga ggtccgtgtc tactagtctc tacggtttcg tgaaagacca
     1621 cgagcctgtg agcccggaag agatctccac cgaattcctc tcgctaacta gcccgaacgg
     1681 tgatgcccgg gctctcgtcg agagactcgt cagaggagat tccaggttca tctgggaggg
     1741 cgagggcttg aggaccgtgc acgccacttc gctcgcgctc ggggaggccc cttacgtcgt
     1801 cttcgacgtg gagactacgg gttccgcggc cgggaagggg ggcgccatca cggagttcgg
     1861 agccctcaag ctcgtgcgcg gggaggtagt agaccagttc tcgacgctcg tcaacccggg
     1921 acgcccgata gacccctttg tggtacgcct gaccggcata acggaccgca tggtctcgga
     1981 cgccccttct atatccgagg tcttgccacg cttcgaggag tttgtcgagg ggtgtgttct
     2041 tgtcgggcac aacgttcatt tcgactgctc cttcgtcact gccgcaagag gtggcactcc
     2101 actccccaac gaggtcctcg acaccctgaa actcgcccgt tgtctggtgc ccggtctcaa
     2161 acgctaccgt ctgagctcgc tcgtcagcca cttcggtgtg cgtcaggcgc cgaaccacag
     2221 ggccctctcc gacgctgctg cgacgacgga ggtgttccgt aaactgctga agctcctagg
     2281 ctccgcaggc ataaagagcg tcggagaagc tctcactatc cgcgggggaa ggggccgcat
     2341 caaaccccag aagcagcacc tggcgagcgg gatgcccaat acgccgggcg tctactactt
     2401 cctcgacaag cacggcacca ccctctacgt cggcaaggcg aaagacctga aggcccgcgt
     2461 caggacctac tttaacggcg gggacggacg ccgcaaggta gggcgtcttg tcgaggaggt
     2521 cgccgaggtg cgcgtcaggg agacagaaag cgagcttcac gccctcgtcc tcgaggccag
     2581 ggagataaag cgcctgttgc cgcgctacaa ctccgtcggc agagacgaca aggcgagctg
     2641 gttcataaga ctcgacctgg gggattccta ccctgttccc gaacgggtct cagagaaccc
     2701 gcccgagggc gcccttattc acctcggtcc ctacaggagc gccgggattt tggacacctg
     2761 catcgaggcg ctcggcagga tcttcccgct caagaggtgc tccggtgatg gggaggtctg
     2821 cttctacggc cagatgggac gctgcgcgcc gtgcataggc atggatgagc acgaataccg
     2881 cgagcgggtc atagacgagt tggtcgcgct tttgcgcggc gagggtggcg aggagcacct
     2941 cagggcgctc gtcagggagc gggaacggct ggccgccggg ctcgagttcg aggccgccgc
     3001 caggctgcga gacctgatct ccggcattga gcggatacgt ttctccagga cggtagtgga
     3061 cgctgagggg gtgcaggccg tcgtcgcccc atcgacggag cccggtgtgg tcgaggtctt
     3121 catcctgtgc gcgggccgtc ttatcgccca cggcggcttc gagccgggcg acgcgtccgg
     3181 actagggcgc ttcgccgacg aggtgctggc caggcctcag gagaacggct ccagtcccaa
     3241 gaaggaaggc tccaacgagg cgcgaatcgt cgcggcttat ctcaggcgcc ggcaggcggt
     3301 ggtcgaggcc gtgcgcttga aagaagcgcg ggatcttctg aaggcggcga agcgggtggc
     3361 tgaggcagcg gccccgctag cttgaggccc gtcggctagg taggttttgt actccaccat
     3421 gctaaaatag taccagagcg aattgtcggt ttctgtgtac aagaagaagg tttagccggg
     3481 aatggtacag gatctacaag gtgtgacgcg caagatactt ctggtcgacg acgagccgtc
     3541 gctccagaag ttgctcacgc acgccctcga gcgcgagggc ttccaggtcc aggtcgctgg
     3601 cgacggcgag gccgccttag aagcgttcaa gacttatgag ccgcacctga tcgtgctcga
     3661 cattatgctc cccaagctcg acggcacgga ggtcataagg aggatcaggg cacagagcga
     3721 cgtgcctgtc atcatgctga ccgcccgcga cgacgagata gacagggtcg tcgggctcga
     3781 gcttggggcg gacgactacg tcaccaagcc tttcgccgtc agggagctgg tggcgcgcgt
     3841 cagggcgatc atgcgcaggg ccgctatacc ggccggccag cgccccgacg agctgaccta
     3901 cgacaggctc aagatcaacc tgcctagccg cagggtgtcg gccgacggtg aagaggtgga
     3961 cctcacctac accgagttcg agctcctggt gaccctggcc tcaagccccg gcagggtgtt
     4021 ctcgcgcagc gcgcttctgc gcagggtgtg gggcgacgag ttccgcgacg aacgcacggt
     4081 ggacgtccat atccgccacc tccgcgagaa gatagagcgc gaccccagga accccgaatt
     4141 catccacacc gcgcggggtg tcggatatgt tttccgctaa agaacggtcg agcccggatc
     4201 tagacttcag gcgaaaggcc agtaagctgt tagccccgaa gacggggggc gagttcaggc
     4261 cccgcctgag catgcgcatc tggctcacgg cgctcttcgt gctcgtaacc gccttcgcgg
     4321 cgataaccgc ttacgagatc gtgcatccca tcctctatga gacactcaac cggtcgagcg
     4381 aggccgcctt caagca
//
LOCUS       DATMJA010000264         2839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_391017, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000264 DATMJA010000000
VERSION     DATMJA010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391017"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..478)
                     /gene="rplN"
                     /locus_tag="VNT12_06290"
     CDS             complement(110..478)
                     /gene="rplN"
                     /locus_tag="VNT12_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWI45761.1"
                     /translation="MIQQESRLRVADNSGARSILTIRVLGGSKRRSAGVGDVIVATVK
                     EATPGSAVKKGDVVRAVVVRTKKETRRDDGSYIRFGENAGVIINPDGAPRGTRIFGPV
                     ARELREKGYTRIISLAPEVL"
     gene            complement(478..744)
                     /gene="rpsQ"
                     /locus_tag="VNT12_06295"
     CDS             complement(478..744)
                     /gene="rpsQ"
                     /locus_tag="VNT12_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWI45762.1"
                     /translation="MQTDTERNRRKERVGLIVSDRADKTVTVSVEALVRHPMYKKRVR
                     RSRKFMVHDERNEARVGDTVRIVETRPLSARKRWRLANIISRAE"
     gene            complement(892..1083)
                     /gene="rpmC"
                     /locus_tag="VNT12_06300"
     CDS             complement(892..1083)
                     /gene="rpmC"
                     /locus_tag="VNT12_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWI45763.1"
                     /translation="MKVAEVRELDVEELEQRLAETRRELFNLRFQHATGQLENTGQLK
                     EVRKNIARILTVLNQKAGE"
     gene            complement(1080..1499)
                     /gene="rplP"
                     /locus_tag="VNT12_06305"
     CDS             complement(1080..1499)
                     /gene="rplP"
                     /locus_tag="VNT12_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWI45764.1"
                     /translation="MLVPKKLKYRKPHRGRMRGKAKGGTEVQFGEYGLQALEPAWITN
                     RQIEAARIAMTRKIRRGGKVWINIFPHKPVTKKPAETRMGSGKGSPEAYVAVVKPGRV
                     MFEMSGVDETLAREAMHLAAQKLPIKTRFLHRGGGQQ"
     gene            complement(1503..2171)
                     /gene="rpsC"
                     /locus_tag="VNT12_06310"
     CDS             complement(1503..2171)
                     /gene="rpsC"
                     /locus_tag="VNT12_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWI45765.1"
                     /translation="MGQKVHPEGFRLGVQRKGEKVDFKSSWYSDRDYAELLGEDIKIR
                     DHIENKLNRAGIADIQIRKAAEQGEIAVDIHTARPGIVIGKGGSEVDALRGDLERLTG
                     KKVQVNVREVSRPELNASLVAESIAEQLVGRAAFRRTMKRALTSAIRSGAEGARIQLG
                     GRLGGIEMSRSETVSEGRVPLHTLDADIDYGFREAKTQMGQIGVKVWINHGVHDEQPE
                     AIRR"
     gene            complement(2172..2537)
                     /gene="rplV"
                     /locus_tag="VNT12_06315"
     CDS             complement(2172..2537)
                     /gene="rplV"
                     /locus_tag="VNT12_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWI45766.1"
                     /translation="MKAKAKYVRIAPRKARLVADEVRGKSYPEAVSLLRFTNKRAAKI
                     IGEIVQSAAANAEHNDDADPDELFVRDIRVDDGPTIKRYRARAMGRATMIRKRTSHIS
                     VELGDPAGIFVSTPGDEEE"
     gene            complement(2534..2815)
                     /gene="rpsS"
                     /locus_tag="VNT12_06320"
     CDS             complement(2534..2815)
                     /gene="rpsS"
                     /locus_tag="VNT12_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWI45767.1"
                     /translation="MTRSSKKEPFVEERLMERILRMNENNEKRMLKTWSRASVIYPEF
                     VGHTIAVHDGRKHVPVYCTESMVGHKLGEFAPTRTFRTHLKGDRTVRRR"
BASE COUNT          381 a          993 c          806 g          659 t
ORIGIN      
        1 acgaccctgt tctctttcgg gatgactcgt tcgatctcgc cgcgtttgcc cttctctttg
       61 cccgagatca caacgacggt atctccgcgc tttagtttca gacccatgat tacaacacct
      121 ccggggcgag cgagatgatc ctggtgtaac ccttctccct tagctcgcgc gcgacgggcc
      181 cgaagatacg cgtcccgcgg ggagcgccat cggggttgat gatcacacct gcgttctccc
      241 caaagcggat gtacgacccg tcgtcgcgtc gcgtctcctt cttggttcgc accacgacgg
      301 cccttaccac gtcgcccttc ttgacggcgc tgccgggcgt ggcctccttg acggtggcca
      361 cgatcacgtc cccgacgccg gcgctgcgcc gcttgctccc cccgagcacg cggatggtca
      421 ggatgctcct ggcacccgag ttgtccgcga ccctgagtct cgactcttgc tgtatcacta
      481 ctcggccctc gagatgatgt tcgcgagccg ccacctcttg cgcgccgaga gcggcctggt
      541 ctctacgatc cgcaccgtat cgcccacgcg cgcctcgttg cgctcgtcgt gcaccatgaa
      601 cttcctggaa cggcgcaccc gtttcttgta catcgggtga cgcaccagcg cctcgaccga
      661 gacggtcacc gtcttatctg ccctgtccga gacgatgagc ccgacccgct ccttgcgcct
      721 gttccgctcg gtgtcggtct ggatctggcc ctcgtcctgg tccagcgcgg ggcccttggc
      781 aggaccagca tcgaggccgg cttctcccgc ctcgatgagg tcctgtggcg tctcggcaac
      841 gggctcctcg gccgtctcag gagtctggtt tttctcttcc tctaccacta cctactctcc
      901 tgctttctgg ttcaggaccg tcaggatcct ggcgatgttc ttcctgacct ctttgagctg
      961 tcccgtgttc tccaactgcc cggttgcgtg ctggaagcgc aggttgaaga gctcgcgccg
     1021 cgtctcggcg aggcgctgct cgagttcctc gacatccagc tcgcgcactt cggccacctt
     1081 cactgctgac caccccctcg atggaggaag cgcgtcttta tggggagctt ctgggcggcg
     1141 aggtgcatgg cctcgcgggc cagcgtctcg tccacaccgc tcatctcgaa cattacacgc
     1201 cccggcttga cgacggcgac gtacgcctcc gggctgccct tgccgctacc catcctggtc
     1261 tcggccggct tcttggtcac tggcttgtgg gggaagatgt tgatccagac cttccccccg
     1321 cgacggatct tccttgtcat cgcgatacgc gccgcctcta tctggcggtt ggtgatccag
     1381 gccggctcga gggcctgcag cccgtactcg ccgaactgca cctcggtacc gcccttggcc
     1441 ttgccccgca tcctgcccct gtgaggcttc ctgtacttga gctttttggg tactaacatc
     1501 gtctaccgcc tgatcgcttc cggctgctcg tcgtgcacgc cgtggttgat ccagaccttg
     1561 accccgatct gtcccatctg ggtcttggcc tccctgaaac cgtaatctat gtccgcatcc
     1621 agcgtgtgca gaggcactct tccttctgag acggtctcgg accggctcat ctcgatgccg
     1681 ccgagcctcc cgcccaactg gatcctggcg ccttcggcac cgctgcggat agcgctcgtg
     1741 agtgcgcgtt tcatggtccg ccggaacgcg gcgcgcccga cgagctgttc ggcgatggac
     1801 tcggccacga ggctcgcgtt gagctcggga cgggagacct cgcgtacgtt gacctgcacc
     1861 ttcttccctg tgagcctctc gaggtcgccc ctcagggcgt ccacctcgct gccgcccttg
     1921 ccgattacga tccctggcct tgccgtgtgg atgtcaacgg cgatctcacc ctgctcggcg
     1981 gccttcctta tctggatgtc cgctataccg gccctgttta acttgttctc gatgtggtcc
     2041 cggatcttga tgtcctcgcc gaggagctcg gcgtagtccc tgtccgaata ccaactgctc
     2101 ttgaaatcta ccttctcgcc cttgcgctgg acgccgaggc ggaagccttc aggatgtact
     2161 ttctggccca tctattcctc ctcgtctccg ggcgtgctca cgaatatgcc ggcagggtct
     2221 ccgagctcga cgctgatgtg gctcgtacgc ttgcggatca tggtcgcccg gcccatcgcc
     2281 ctggcgcggt aacgcttgat ggtcggcccg tcgtcgaccc ttatgtccct gacgaacaac
     2341 tcgtcggggt ccgcatcgtc gttatgctct gcgttggcgg cggcgctctg gactatctcg
     2401 ccgatgatct tcgcagcccg cttattggta aagcgcagga gcgagaccgc ctcgggatag
     2461 ctcttgcccc tgacctcgtc tgccacgagg cgcgccttgc ggggagcgat acggacgtat
     2521 ttggccttcg ccttcatcgg cgcctcaccg tcctgtcacc cttgaggtgc gtgcggaagg
     2581 tacgggtcgg ggcgaactcg ccgagcttgt ggccgaccat cgactctgtg cagtacaccg
     2641 gtacgtgctt gcgcccgtcg tggacggcga tggtatggcc gacgaactcg gggtagatca
     2701 cactcgcacg gctccacgtc ttgagcatcc gcttttcgtt gttctcgttc atccggagga
     2761 tgcgctccat aaggcgctcc tccacgaacg gctctttctt agaggaccgc gtcatctagc
     2821 gcctccttcc tttcctgcg
//
LOCUS       DATMJA010000265         2364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_805943, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000265 DATMJA010000000
VERSION     DATMJA010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805943"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VNT12_06325"
     CDS             <1..757
                     /locus_tag="VNT12_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HWI45768.1"
                     /translation="ELEEGTPLVAKVDRAGESHLMREAYMLRYLREKSDLPVPEVYHA
                     SETLLLMEFVEESSRFSKKAQHHAAELLAALHEITSATYGHERDTLIGSLSQPNPPTE
                     SWTEFFREHRLLYMANVANEAGRLPAEDLLRVERLSEKLDELIEDPNPPALIHGDVWS
                     ANVLARGDRIAAFLDPAIYYADPEIELAFISLFDSFSNTFLERYAEIRGIDKAFFQTR
                     RDLYNLYPLLVHTYYFGGGYLNSVRNTLDRFGI"
     gene            complement(953..1420)
                     /locus_tag="VNT12_06330"
     CDS             complement(953..1420)
                     /locus_tag="VNT12_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HWI45769.1"
                     /translation="MSASRALGLGAISGLRALSGPAFVSRAASRGDLNLDGTFLAFLS
                     SPRLSKALVLMELGELVGDKLPATPSRTSPPPLLGRAASGALVGAAVFVSEGLRATTG
                     AALGSTAAIAAGFTGEWLRALTVEKTGFPDPVVALAEDAIVLLVGLQSSKDIR"
     gene            complement(1417..>2364)
                     /locus_tag="VNT12_06335"
     CDS             complement(1417..>2364)
                     /locus_tag="VNT12_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWI45770.1"
                     /translation="LENRRATDNEVVHEAAAAMKETGFGIKAATVTPEDPGDVGSPNA
                     ILRKGIDGTVIVRTGRRIPGVNPTSGVHAPISVVRMAVDDAYGAKEWREGEGLDEVAY
                     RTEKISRRVCRGVAEFAFIQARKMRAKVFGGPKYTVSPVYEGMLKEEMDRAAENSRDV
                     RYDPQLIDATYALLLTTYGEPLVIPTLNRDGDCISDLVMQMFGSIAGAESLLISLDEE
                     ENPETVMAEAPHGTAPALEGKNVANPMAMILAGAALLTFFHDEDASRVSRAIYEATFE
                     TILDGVKTADLGGSADTTEFTDEIIRHIRTKLEVWSALA"
BASE COUNT          435 a          745 c          746 g          438 t
ORIGIN      
        1 ggagctggag gaaggcaccc cgctcgtcgc caaggtagat agggcaggcg aatctcatct
       61 gatgcgcgaa gcatacatgc tccgctacct gcgtgagaag agcgatctcc ctgtccccga
      121 ggtctaccac gcttcggaga cgcttctctt gatggagttc gtcgaagaga gcagccgctt
      181 ctcgaaaaag gcccagcacc acgccgctga actcctggca gcgttgcacg aaattacttc
      241 cgctacctac ggccacgagc gggacacgct catcggtagt ctgagccagc ccaaccctcc
      301 gaccgagagc tggacggagt tcttcaggga gcacagacta ctgtacatgg cgaacgtcgc
      361 taacgaggcg gggcgtctac cggcggagga cctgcttcgg gtggagcggt tgtcggagaa
      421 gctcgatgag ctcatcgagg atcccaaccc tccggcgctg atccacggcg acgtctggag
      481 cgccaacgtg ctcgcacgag gagatcgcat agccgctttc ctggaccctg caatctacta
      541 cgccgacccg gagatcgaac tggccttcat cagcctcttc gactctttca gcaacacttt
      601 tctggagcgc tacgctgaga tacggggcat tgacaaagcc ttcttccaga cgcgccgtga
      661 cctgtacaac ctctacccgc tgctggttca cacctactac ttcggaggcg gttacttgaa
      721 ttcggtaaga aacacactgg accggttcgg catttaggct acttcctctc agcgacgtac
      781 agggtggtca ggtagccctt gacgatacgg ccgccgaacc tcgtggcgat aaggtcagcg
      841 atgctgtgaa agaggcgctc gcgggtgtcg tcgcctagac tccgtcgcag agcgaggccg
      901 cttcatcgaa agtcctcctg agcctgttcc tatcatcggc catgagcctt cgctaacgga
      961 tgtcctttga ggactggaga ccgacgagca agacgatggc gtcctccgcg agcgcgacga
     1021 cggggtcggg gaacccggtc ttctcgactg tgagcgcacg taaccattcc cccgtgaagc
     1081 ccgcggcaat ggccgcggta gaacccagtg ccgccccggt cgtggcgcga aggccttccg
     1141 agacgaagac ggctgccccc accagggctc ctgaggccgc acgcccgagc aggggaggtg
     1201 gggaggtacg gctcggtgtg gcgggcaact tgtccccgac caattcaccc aactccatca
     1261 atacgagcgc cttcgagagg cgcggcgagc tcaggaaagc gaggaaggtt ccgtcgagat
     1321 tcaagtcgcc ccggcttgcc gcgcgactga cgaaggcagg accagagaga gcgcgcaggc
     1381 ccgagatggc gccgagaccg agggctctag aagcgctcac gcgagcgcgc tccacacttc
     1441 gagcttggtc ctgatgtggc gtatgatctc atcagtgaac tcggtagtgt ccgccgaacc
     1501 accgaggtca gcggtcttca caccgtcgag gatggtctcg aaagtcgcct cgtagatggc
     1561 gcgtgagacg cggctcgcgt cctcgtcgtg gaagaacgtg aggagcgcgg ccccggccag
     1621 gatcatggcc atggggttgg cgacgttctt gccctctagc gcaggagccg tgccgtgcgg
     1681 cgcctcggcc atgaccgtct cggggttctc ctcctcgtcg agcgagatca ggagcgactc
     1741 ggcccccgct atggagccga acatctgcat cacgaggtcc gagatgcagt caccgtcacg
     1801 gttgagggtg gggatgacga ggggctcgcc gtaagtggtc aagagcagcg cgtaggtggc
     1861 gtctatgagc tgggggtcgt agcggacgtc gcggctgttc tcggctgctc ggtccatctc
     1921 ctctttcagc atcccctcgt agacgggtga gacggtgtac ttgggaccgc cgaacacctt
     1981 ggcccgcatc ttgcgcgcct ggataaaggc gaactcggcg acgccacggc ataccctccg
     2041 tgagatcttc tccgttctgt aagcgacttc gtcgagccct tcgccctcgc gccactcctt
     2101 cgcaccataa gcgtcgtcga cggccatgcg cacgacggag atgggggcgt gtacgccgct
     2161 cgtcgggttg acgccgggga tgcgccgtcc cgtgcgcacg atgacggttc cgtcgatgcc
     2221 tttgcgcaag atggcgttgg gcgaacccac gtcgccaggg tcctccggcg tgacggtggc
     2281 agcctttatg ccaaagcccg tctctttcat tgcagcggcc gcctcgtgca cgacctcgtt
     2341 gtctgtggcc cgccggttct ccag
//
LOCUS       DATMJA010000266         3726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_154520, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000266 DATMJA010000000
VERSION     DATMJA010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154520"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /gene="dut"
                     /locus_tag="VNT12_06340"
     CDS             complement(<1..357)
                     /gene="dut"
                     /locus_tag="VNT12_06340"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWI45771.1"
                     /translation="MSSERPLQVPFRRLVPEAGMPERAHVGDAGYDLRSVERVDLAPG
                     SRALVRTGIAVAIPEGYAGLVLPRSGLAVRHGISLVNTPGLIDSGYRGEILVPLINHD
                     REETFLIEEGTRIAQLV"
     gene            499..2223
                     /gene="metG"
                     /locus_tag="VNT12_06345"
     CDS             499..2223
                     /gene="metG"
                     /locus_tag="VNT12_06345"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWI45772.1"
                     /translation="MSEQRRRYLVTSALPYANGPLHVGQISGAYLPADAFVRYLRARG
                     EDVVYTCGTDEHGVPITLTAEREGKDPQEVVDYWWTNMKETFERFGISFDNFSRTSTP
                     LHAKNTQLFYRKLKEGGYISEAESKQMYSPTEGRFLPDRYVEGICPVCGYKEARGDQC
                     DNCGSWYEAYELIEPHSKVTGGPVEVRETRHLYLDLPEFSERLREYVAGQEHWRTSVK
                     NFILGIIDGGLEKRPITRDIEWGVPVPEPGFEDKRFYVWFDACIGYVSSTMEWAENIG
                     DPDRWREYWQEPESRLIHFIGKDNTVFHTLIWPAQLMGTAEDGEEPFVLPYDVPANEF
                     MNLEVLVDGERRAMQMSTSRNLAVWLHEALDRFPADPLRFYLASNLPETGDVVFAWRD
                     FQSRVNSDLIGNLGNYINRVLSFAERYFDGDIPSPEGLPEDAVAVLEDFRELEVRYEA
                     KMLAPRPREALAELLAMGRSANRFFDASAPWKTRKEDPEQTRLTLYTCSVLLGSLAYH
                     AAPYVPEAIGRLEEFFDGPIARVSDLDELPKGYHAAGAKPLFRRIEDDEVAAAEGQLY
                     RAVSGEAG"
     gene            complement(2255..3190)
                     /gene="thyX"
                     /locus_tag="VNT12_06350"
     CDS             complement(2255..3190)
                     /gene="thyX"
                     /locus_tag="VNT12_06350"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWI45773.1"
                     /translation="METTERNAERAQLLFEELGGTVNDGFPTIHSQVHHTEVGTPYLK
                     TPGVVVLARPQTNIAVLGGFLEGFDPELNFAGYVNDPTLLPDSSQLCKTAGQVCYSSF
                     GPRRTRNENADAYFERLTSAGHGSVLEHASFSFLLYGISRSVTHELVRHRAGAAFSQI
                     SQRYVSGSVLRFVERPEYQEDDELHQIFEERADRAAAEYEAMAEQLLERQEGGASMLT
                     ADFKTDARKKVQQTARSLLPNETEAPMVFTGNVRALRHIIEMRADAHAESEIRNLALR
                     IFLCLRTVDPILFGDYELAELPDGTYTVSTKNRKA"
     gene            complement(3249..>3726)
                     /locus_tag="VNT12_06355"
     CDS             complement(3249..>3726)
                     /locus_tag="VNT12_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 domain-containing protein"
                     /protein_id="HWI45774.1"
                     /translation="DALYRLRRAENALSIELGRDATEEELVERLGVKPEEARRLREVS
                     QPISSINAKINADEGSEFGELLPDERSGDDYARVEVGLWELALVEAVKSLPEREARII
                     EMRHGLDGSDTLTLREVSEELGISQERARQVEIKALRTIRTGRYASTLRRALSEAV"
BASE COUNT          681 a         1160 c         1170 g          715 t
ORIGIN      
        1 gacgagctgc gcgatcctcg ttccctcctc gatcaggaag gtctcctcgc ggtcgtggtt
       61 tataagggga accaggatct caccgcggta gcccgagtcg ataaggcctg gcgtgttgac
      121 gagggagatc ccatgcctca ccgcgaggcc gctgcgcggg agcacgagcc cagcgtaacc
      181 ctcagggatg gcgaccgcga tccctgtccg caccagagcc cgggatcccg gcgccagatc
      241 caccctctcg accgaacgca gatcatagcc cgcatccccg acatgggcgc gctcgggcat
      301 ccccgcctcg gggacgagac ggcgaaaagg gacttgaagc ggacgctcgg agctcatccg
      361 gagaatggta gcactggaag gtttcaggct acaggcagct ccagcgatac gccaccgcgc
      421 aagactaaca ccgaggcaat gcccctacat gacggctgac acctgatgcc tgtagccttc
      481 tgctgttatc cttagtttat gagtgagcag agacgacggt atctcgtaac ctcggcgttg
      541 ccttatgcaa acgggccgtt gcatgtaggg cagatctcgg gcgcttacct gcccgctgac
      601 gcgttcgtgc gttatctgcg agcgcgtggc gaggacgtgg tctacacctg cggcaccgac
      661 gagcacggag tgccgatcac cctgacggca gagcgtgagg ggaaggaccc acaagaggtc
      721 gtcgactact ggtggaccaa catgaaggag actttcgagc gcttcggcat ctccttcgat
      781 aacttctcgc gcacctcgac cccgcttcac gcgaagaata cccagctctt ctaccgcaag
      841 ctaaaggagg gcggctacat ctcggaggcc gaatcaaagc agatgtacag ccctacggag
      901 ggccgttttc tgcccgaccg ttacgtagag gggatctgcc ccgtgtgcgg ctacaaagag
      961 gcgaggggcg accagtgtga caactgcggc tcgtggtacg aagcgtacga gctcatcgag
     1021 ccgcactcca aggtcacggg cggacctgtc gaggtcaggg agactaggca cctctacctc
     1081 gacctccccg agttctccga aaggctgagg gagtacgtcg ctggacagga gcactggcgc
     1141 acgtctgtca agaacttcat cctcggcatc atagacggcg gcctggagaa gcggcccata
     1201 acgcgggaca tcgagtgggg cgtccccgtc cccgagcctg gcttcgagga caagcgcttc
     1261 tacgtgtggt tcgacgcctg tatcggctac gtctcctcga cgatggagtg ggcagagaac
     1321 attggggacc ccgacaggtg gagggagtac tggcaggagc ctgagagccg gctcatccac
     1381 tttatcggga aggacaacac ggtcttccac accttgatct ggcccgccca gctcatgggc
     1441 accgcagaag atggtgagga accgtttgtc ctaccctacg acgtgcctgc caacgagttc
     1501 atgaacctgg aggtcctggt cgacggggag cggcgggcga tgcagatgtc cacgagccgt
     1561 aatctggccg tctggctgca cgaggccctc gaccgcttcc ccgccgaccc tctgcgtttc
     1621 tacttggcct cgaacctgcc cgagacgggg gacgtggttt tcgcctggcg ggatttccag
     1681 agcagggtga actcggatct gatcgggaac ctcggcaatt acatcaaccg cgtcctctct
     1741 tttgcggaga ggtacttcga cggcgacatc ccaagtccag aggggctacc tgaagacgcc
     1801 gtcgcagtcc tggaggattt cagggagttg gaggtgcgct acgaggcgaa aatgctcgcc
     1861 ccgagacccc gcgaagcgct cgcagagcta ctggcgatgg gcaggagcgc caaccggttc
     1921 ttcgacgcgt cggcgccctg gaagacccgc aaggaggacc ctgagcagac ccgcctcacg
     1981 ctctatacct gctccgtctt gctcggctcc ctcgcctacc acgccgcgcc ttacgtccct
     2041 gaagctatcg ggcggctaga ggagttcttc gacgggccta tagcccgcgt ctcggacctc
     2101 gacgagctgc cgaagggtta ccacgctgcg ggggcgaagc ctctgttccg caggatcgag
     2161 gacgatgaag tcgcggcggc cgaggggcaa ctctacaggg ccgtcagcgg cgaagcgggt
     2221 tagacggagt tcgggcttcc tccttattgc aggcctatgc cttgcggttc ttggtcgata
     2281 cggtatacgt tccgtcggga agctccgcca gctcgtagtc gccgaacagg atgggatcca
     2341 ctgtccgcag gcacaggaag atgcggaggg ccaggttgcg gatctcgctc tccgcgtgcg
     2401 cgtcggcgcg catctcgatg atgtgccgta gcgcgcgcac gttccccgta aagaccatcg
     2461 gagcctctgt ttcgttcggc aacaaggagc gtgcggtctg ctgcaccttc tttctcgcgt
     2521 cggtcttgaa gtctgcggtg agcatgctcg ccccgccctc ctggcgctcc agcaactgtt
     2581 cggccatcgc ctcgtactcc gccgcagccc tgtcagcccg ctcctcgaag atctggtgca
     2641 actcgtcgtc ttcctgatat tccggacgct ctacgaagcg tagtacactt cctgatacat
     2701 agcgttgcga aatctgggaa aaagcggcgc ctgcgcgatg tctcaccagt tcatgggtca
     2761 cgctgcggct gatcccgtaa agcaggaagc tgaagctcgc atgttccaga actgaaccat
     2821 gtccggcgga ggtcaggcgc tcgaagtacg cgtcggcgtt ctcgttcctc gtgcgccggg
     2881 gcccgaagct actgtagcac acttggcccg ccgtcttgca gagctgtgaa gagtcaggga
     2941 gcagcgtagg gtcgttaacg tagccggcaa aattgagttc ggggtcgaag ccttcgagga
     3001 atcctccgag caccgcgatg ttggtctggg gtctcgctaa tacgacgacc cctggtgtct
     3061 tcagataagg tgttccgacc tcagtgtggt ggacctgtga gtggatcgtg gggaagccgt
     3121 cgttgacagt cccgcccaac tcttcgaata gcaactgcgc ccgctcggcg ttacgctccg
     3181 ttgtctccat gggctccctt cgccgttgtg cctctggaga gcaggttagc agcccattag
     3241 attctgttct acacggcctc agagagggcg cggcgcaggg tgctggcgta gcggccggtg
     3301 cggatggtcc tcagagcttt gatctcgacc tgccttgccc tctcctggga gatgccaagc
     3361 tcctccgaga cctcgcgcag ggtgagggta tcgctgccgt cgaggccgtg gcgcatctcg
     3421 attatcctgg cctcccgctc gggtagagat ttcacggcct cgacgagcgc cagctcccac
     3481 aggccgacct cgacgcgcgc gtagtcgtca ccggatcgct cgtcagggag caactcgcca
     3541 aactcggagc cttcgtcggc gttgatcttc gcgttgatgc tggagatggg ctggctgacc
     3601 tcgcgcaggc gcctggcctc ctccggtttt acaccgagcc tctcgacgag ctcctcctcg
     3661 gtagcgtcgc ggccgagctc gatgctgaga gcgttctcgg cgcgtcgcag gcggtagaga
     3721 gcgtca
//
LOCUS       DATMJA010000267         2751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_35789, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000267 DATMJA010000000
VERSION     DATMJA010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35789"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VNT12_06360"
     CDS             <1..762
                     /locus_tag="VNT12_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI45775.1"
                     /translation="FMRVFTGKVARRAAPPHRGDNKKVMRILVIEDEARLARLISRVL
                     SEEGYAVETEANGRQALVRALADEFDLLIVDWMLPEMSGVQLVKRLRAAEVGTPAIML
                     TARDQIEDRVEGLDAGADDYLPKPFAFPELLARVRALARRPWTEEKDGAILGAGDVTL
                     DPVRHVVRHRGETVDLTAKEFALLATLLQRPGQVFTRPVLLDTVWGASPEVYANVVDL
                     YISYLRKKLDGDGETSHIRTVRGVGYTFEPQPGPR"
     gene            762..2045
                     /locus_tag="VNT12_06365"
     CDS             762..2045
                     /locus_tag="VNT12_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI45776.1"
                     /translation="MFDHIRRRLTVGYVGILALILLIFGVVVVFTFYLRASDQQDDLL
                     LQKAEDKANSVLSGKDRYGVVNATTDYDVAVIVLLPDGSTYGDLDETSFSLGLPYAYM
                     AQRAGQERQTIKETVPGPDGAVRVMSIPVKKYGEFGSVVAVVQAAQSRQAVTQTVERL
                     IFVLVLTGFGALILATAGGLFMSRRAMRPVQEAFGRQRTFIADASHELKTPLTLIRAD
                     AEVLSRGIDDPDKASDNRELLDDLLGETDRMSAVLSDLLLLARLDAQKVSVSREPFDL
                     ALVISETSERFAARARAEDKNLEVHHSGKLPVRGDAPRTGQSLAALLDNALCFTPPGG
                     TIAVEGRITNQHVEATVTDTGPGIAPESLDRIFERFYRADAHSAARSRGSSGGGTGLG
                     LAIARDLVRAQGGEVSAENTEGGGARFTLSLPTGS"
     gene            complement(2094..>2751)
                     /locus_tag="VNT12_06370"
     CDS             complement(2094..>2751)
                     /locus_tag="VNT12_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI45777.1"
                     /translation="FYAEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGAIGTIQAT
                     ETVKLILGIGEPLIGRLLLYDALNMRFREMKLRKDPDCPVCGENPTVTELIDYQEFCG
                     IPQTEAAEQENGVPEITVGELKTRLDKGEDINVLDVREPHEYEVANIGVKLIPLGELP
                     QRLAELDQDEYFVVHCKTGGRSSKAVKLLQDAGFQNVYNVKGGITAWSEEIDPSVPKY
                     "
BASE COUNT          502 a          871 c          868 g          510 t
ORIGIN      
        1 ttcatgaggg ttttcacagg aaaggtcgcg cggagggcgg cgccgccgca caggggcgat
       61 aataaaaagg ttatgcggat actggtgata gaggacgaag cgaggctggc gcgtctgatc
      121 tcgcgtgtcc ttagcgagga gggctacgcg gtcgagacgg aagccaacgg ccgtcaggcg
      181 ctcgtgcgtg ccctggcaga cgagttcgac ctcctgatcg tggactggat gctccccgag
      241 atgagcggcg tccagttggt aaagcggttg agggcggccg aagtcgggac tccggcgatc
      301 atgctgacgg cccgcgacca gatcgaagac cgtgtggaag gcctcgacgc cggcgccgac
      361 gactacctcc ccaagccttt cgccttcccc gaactgctcg cccgggttcg ggccctcgcc
      421 cgccgtccgt ggaccgaaga gaaagacggc gcgatcctcg gggccggcga cgttacgctt
      481 gatcctgtgc gtcacgtggt tcgccacaga ggagagacgg tagacctgac ggccaaggag
      541 ttcgcactgc tcgccacact gctccagcgc cccggacagg tcttcacccg cccggttctg
      601 ctcgataccg tgtggggcgc ctcacccgaa gtctacgcca acgtcgtcga cctctacatc
      661 tcctacctgc gtaagaaact cgacggcgac ggcgagacct cccacatccg aaccgtccgc
      721 ggtgtcggct acaccttcga accgcaacca ggaccgcgct gatgttcgat cacatccggc
      781 ggcgcctgac ggtgggctac gtaggcatac tggctctcat cctgttgatc ttcggcgtcg
      841 tagtggtgtt cacgttctac ctgcgggcaa gcgaccaaca agatgacttg ctcctgcaga
      901 aggcggaaga caaggccaac agcgtgttga gtggcaaaga cagatacggc gtcgttaacg
      961 cgaccacaga ttacgacgtg gccgtgatcg tacttctgcc tgatggttcg acctacggag
     1021 atctggacga gacgtccttt tccctcggcc tcccctacgc atatatggcc cagagagccg
     1081 ggcaggagag gcagactatc aaagagaccg ttcccggccc tgatggcgcc gtgagggtga
     1141 tgagcattcc cgtaaagaag tacggagagt tcggaagtgt ggtcgcggta gtccaggctg
     1201 cccagtctcg tcaggctgtg actcaaacag tggaacggct gatcttcgtg ctggtcctca
     1261 cgggctttgg agcgttgata ctggcgacgg ccggcgggct tttcatgtcg cggcgggcga
     1321 tgcgtccggt ccaggaagcg ttcggcaggc agaggacgtt tatcgctgat gcctcccacg
     1381 agttgaagac gccactgacc ctgatccggg ccgacgccga ggtcctctca cgcggcatcg
     1441 acgaccccga caaggcctct gacaaccgtg aactcctcga cgacctgctc ggcgagaccg
     1501 acaggatgag cgccgtgctc tccgaccttc tactgctcgc gcgtctggac gcgcagaagg
     1561 tctccgtctc ccgcgaacct tttgacctcg ccctcgttat ctccgagacc tcggagcgat
     1621 tcgccgcccg cgccagggcc gaggacaaga acctcgaggt tcaccactca ggcaaacttc
     1681 ctgtgcgcgg cgacgcgccc cgtacgggcc agagcctcgc cgccctcctc gataacgccc
     1741 tctgcttcac acctcctggc gggaccatag cagtggaggg ccgtatcacg aaccaacatg
     1801 tcgaggcgac cgttacggat acagggccag gtatagcccc cgagagcctg gatcgcatct
     1861 tcgagcgttt ctacagggcc gacgcgcaca gcgcagccag gagcagggga tcttccggcg
     1921 gcggaacggg cctcggtctc gccatcgccc gcgatctcgt ccgcgcccag ggcggtgaag
     1981 tcagcgccga aaataccgaa ggaggcgggg caaggttcac gctaagcctg cccactggtt
     2041 cctgacctca cttcggccag gtagctcgca gaaccctgag acctgtagcc tccctagtat
     2101 ttcgggacgc ttggatcgat ctcctcgctc caggctgtaa tgccgccctt gacgttgtag
     2161 acgttctgga agccagcgtc ttgcaaaagc ttgaccgcct tcgagctgcg tccgccggtc
     2221 ttgcagtgaa cgacgaagta ctcgtcctga tccaactccg ccaggcgctg cggcaattca
     2281 cccaagggta ttagcttcac gccgatgttg gcgacctcgt actcgtgcgg ctcgcgcacg
     2341 tcgaggacgt tgatgtcctc gcctttatcg agcctggtct tgagctctcc gaccgtgatc
     2401 tcaggcacgc cgttctcctg ctcggcagcc tcggtctgcg ggatgccgca gaactcctgg
     2461 tagtcgatca gctccgtaac cgtcgggttc tcgccgcaga cggggcagtc cgggtccttg
     2521 cggagcttca tctcgcggaa gcgcatgttc agcgcgtcgt agaggaggag ccgtccgatg
     2581 agcggctcgc cgattccgag tatgagcttg acggtttcgg tggcctggat ggtgccgatg
     2641 gcgccgggta agatacccag cactccgccc tctgcgcagc tggggacgag gccaggaggg
     2701 ggtggctcgg ggtagaggca gcggtagcag gggccttcct cggcgtagaa g
//
LOCUS       DATMJA010000268         2687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_273020, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000268 DATMJA010000000
VERSION     DATMJA010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273020"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /gene="hflX"
                     /locus_tag="VNT12_06375"
     CDS             <1..898
                     /gene="hflX"
                     /locus_tag="VNT12_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWI45778.1"
                     /translation="DLEYRLPRVKGKNPELSRLGGGGVTTRGPGEQQLEYDRRVIRER
                     IATINRKLEEEKAGRAVRGARLREEGPPTVALVGYTNAGKTTILNALSGARRSTKDRL
                     FETLETTTRLVEGSRKDGPDGMWPDFVVTDTVGFIRKLPTQLVHSFASTLEVARASDV
                     TVVCADASSEELEEEIQTVTRTLSGAVENGAGSGPMLMCLNKMDLVTGARAAELRRDH
                     SHAVTISATLDCEALVEEIYRAIASRRERMRVFIPHAEYAAASRLYGLAEIYAQENTE
                     SGLWMDVSLPRSVSGKYAPYRV"
     gene            895..1668
                     /locus_tag="VNT12_06380"
     CDS             895..1668
                     /locus_tag="VNT12_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HWI45779.1"
                     /translation="MKVLRLYPTPGREVSAIYEDLDLPPTGRRDAARPYVIINSVSSV
                     DGRTVMEGKAAGIGSTTDRRTMRTLRSRADAVMIGAGTLRTERLSLGLDPTDGVPQPL
                     AIIVTDTGDVPLEEQLNVEGGQEVLILLSDAAPQRVAERLRSLAPVMRAPAGSTGAVD
                     LEAALRALEAERGIESLVVEGGPALNHALISRQLVDELFLTVAPELLGGTAAQTLTLL
                     RGPEIPARDRPVLELVSIHLAESELFLRYSLTRTPPGSA"
     gene            1907..2050
                     /locus_tag="VNT12_06385"
     CDS             1907..2050
                     /locus_tag="VNT12_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45780.1"
                     /translation="MIQLRVRWARSPRQAEERAVKVGRNQVEFIRVYRERVIPKLDWP
                     EKG"
     gene            2055..>2687
                     /locus_tag="VNT12_06390"
     CDS             2055..>2687
                     /locus_tag="VNT12_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI45781.1"
                     /translation="MGWKNGGYVSVLLVALGASLWGTDAILRVPLLEVASPSQIVLLE
                     HLVLLLYSVPAVALGWRFFHGLGAAQWVALLIIGWGGSALATLLFTTSFAVGNPTVAI
                     LLQKTQPLFAVALAGILLRERLEWAYWPCFAVAVIGAYLISFGDLGPFRAYSSAEALT
                     AALALGAALLWGSSTVLGRLVLKDMPFHALTGARLLLAVPLLAAIVAFQGS"
BASE COUNT          487 a          818 c          901 g          481 t
ORIGIN      
        1 cgacctggag taccgtttgc caagggtcaa gggtaagaac ccggagttga gccgcctcgg
       61 aggcggaggg gtcactacga ggggccccgg cgagcaacag ctggagtacg acaggcgtgt
      121 catccgcgag cgcatagcga ccataaaccg caagctcgag gaggagaagg caggtagggc
      181 cgtacggggc gcgcggctga gagaggaagg accgccgacc gtcgcgctgg tgggctacac
      241 gaacgcaggc aagactacca tcctgaacgc cctcagcggg gcgaggaggt cgaccaagga
      301 caggctcttc gagacgctag agactactac gcgtctagta gaggggagca ggaaagacgg
      361 gcccgacggc atgtggcccg acttcgtggt caccgacacc gtggggttca taagaaagtt
      421 acccacgcag ctcgtacact ccttcgcctc gacgctcgaa gtggcgcgtg cttccgatgt
      481 cacggtcgtt tgcgcggacg cctcgagcga ggagctggag gaggagatcc agacggtgac
      541 gcgcaccctc tccggggctg tcgaaaacgg ggctggctcg gggccgatgc tcatgtgcct
      601 caacaagatg gatcttgtta caggggcgcg cgccgctgag ttgcgcaggg atcactcgca
      661 cgcggtcacc ataagcgcca ccctagactg tgaggcgctg gtggaggaga tctaccgcgc
      721 catcgcgtcc aggagggaga ggatgcgagt ctttatcccc cacgccgaat acgctgccgc
      781 gagtcgcctt tacggcctgg cggagattta cgcccaggag aacaccgagt caggcctgtg
      841 gatggacgtc agcctgcctc gttcggtctc gggcaagtac gctccctaca gagtgtgaag
      901 gtactgaggc tgtatccgac ccctgggcgg gaagtctccg ccatctacga agacctggac
      961 ctgccgccca cagggcgccg cgacgcggcc aggccgtacg tgatcatcaa ctcggtcagc
     1021 tccgtggatg gtaggacagt aatggagggg aaagccgccg gaataggaag cacgaccgac
     1081 cgccggacga tgcggaccct tcgctccagg gccgacgccg tgatgatagg cgccggtacg
     1141 ctgcgcaccg agaggctctc actcggcctc gacccgacgg atggtgtacc gcaaccgctc
     1201 gcgatcatcg ttaccgacac gggcgacgta ccgctggagg aacaacttaa cgtcgaggga
     1261 gggcaggaag tcttgatact gctctccgat gcggctcccc agagggtggc cgaacggctg
     1321 cgcagtctag cgccggtgat gagggcgcct gcgggctcca ctggtgccgt cgacctcgag
     1381 gcagccttgc gggccctcga agccgagcgt ggtatcgagt cgctagtggt cgaaggcggg
     1441 cccgctctca accacgctct gatctcgcgt cagctcgtcg acgagctctt ccttaccgtg
     1501 gcgcctgaac tgctcggcgg aaccgcggcc cagaccctta ccttgctcag aggtcctgag
     1561 atcccggccc gcgaccgtcc cgtcctcgaa ctagtgtcca tccatctggc ggagagtgag
     1621 ctgttcctgc gatactcgtt gacccgaact ccaccaggct ccgcgtaggt ttcggccggt
     1681 ggtgcggcga cgacctgcca agccggcgga caggtcgatc tcaagacgac ctggagggtc
     1741 atcgccgctt taattctcgc ggatgcatct cgtttggctg ctacccgtac cccaaacccg
     1801 agaacacggg tagaatcgga cggagagtac gcacccgaca ggagggtacg atgcaaatca
     1861 ccggaaccgt cgagagaccg acgaagacac ggcgcggatg ccgcgcatga tccagctccg
     1921 cgtacgctgg gcccgatctc caaggcaggc cgaagagcgg gccgtgaaag tcgggcgcaa
     1981 ccaggtggag tttattaggg tctataggga gcgtgtgatt cccaaactcg actggcccga
     2041 gaagggttag cggcgtgggc tggaagaacg gtggttacgt tagcgtcttg ctcgtcgccc
     2101 ttggggcttc gctctggggg acggacgcca tcttgcgggt accgctcctg gaggtagcgt
     2161 cgccgtccca gatagtgctg ctcgaacacc tcgtattgct tctgtactcg gtgccggccg
     2221 tggcgctggg atggagattc ttccacggct taggggccgc gcagtgggtc gcgctgctca
     2281 tcatcggttg gggaggctca gcccttgcga cgctgctttt tacgacctcg ttcgccgtgg
     2341 ggaatccgac ggttgcgata ctgttgcaga agacccagcc gctgttcgca gtcgcgctgg
     2401 cggggatttt gctgcgcgag cggttggaat gggcttactg gccgtgtttc gccgtggcgg
     2461 tgattggggc ttacctgatc tccttcggcg acctcggacc tttcagggca tatagctccg
     2521 ccgaagctct caccgcggcc ctcgcccttg gcgccgcgct cttgtggggc tcctcgacag
     2581 tgctgggacg gctggtcttg aaagacatgc cgttccatgc tctgaccgga gcgcgactcc
     2641 tgttggccgt gcccctgctc gcggccatcg tagctttcca gggctcc
//
LOCUS       DATMJA010000269         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_569379, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000269 DATMJA010000000
VERSION     DATMJA010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569379"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VNT12_06395"
     CDS             complement(<1..843)
                     /locus_tag="VNT12_06395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI45782.1"
                     /translation="MEHRRLGRLDRENSVLIFGGAALAEATEEDSDVAIDQALDAGVD
                     HFDTAADYGDSELKYGRWMPEIRDRIFLSTKTGLREKDAAKRQIQDSLERLRVDNVDL
                     LQLHSVGDIEDLDRATGPGGSLEAAIEAKEEGLIGAIGITGHGNEAPATHLEALRRYP
                     FETVLTPWNYILSTDEGYRADYEALVEEVRRQDAGLMIIKTISRRNWPEGDPTGDQRY
                     TTWYEPFDRQEYIDAAVSWVLSHDEVTGLAMVGDLTLVPMMLKAENRRMSREEAEQVL
                     SRAPD"
     gene            complement(867..>2757)
                     /locus_tag="VNT12_06400"
     CDS             complement(867..>2757)
                     /locus_tag="VNT12_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HWI45783.1"
                     /translation="IAHDFENPGQDYNGNITPHDIYGTWRNFEGKRHHPESLFSFGWG
                     DGGGGPSEQMLENYARMKSFPAMPRLRMAHVEEFFESLPKEGLPRWTGELYLELHRGT
                     LTTQARVKKLNREAEHRLLEAEALATLAALHGGEYQREELEAAWKTLLLNQFHDILPG
                     TSIGEVYDVTHKELADVVRTARKLRDSALSPPPEAEPALLVANVSLNPRPLSVLLDAE
                     SKPEGTSGVADADGRPLPTQVTEGGELLVHAPDLSVPGIGWSTLTARHESAPARREGQ
                     DVGAEVAGGRAVLDNGLLRVEVGEDGTLHGVYDREAGREVLAGRANQLWAYTDKPRNW
                     EAWDVDEGYEKEGEEISSVESIEVTESGPLRASVGVIRSWRDSIIAKTYRLLSGSRRL
                     DVEAYVDWRERRVLLRTLFPLAIRSHETTSETMFGALRRPTHHNTPWDATRFEVSAHR
                     FCDISEPGYGVALLNDGKYGHSAKGNVVGISLLRSPLYPDPFADEGGHRFTYSLFPHP
                     GDWTTAGVAREAFALNAPLFPVRSQEDALPRGFSLVEAEGPELALGSLKLAEDGDSVI
                     LRLYEPHGARGECTVRFARPVDRVERVNLLEEAEKPVEVHRGAVRLRVRPFEVVTLRV
                     HWERE"
BASE COUNT          426 a          882 c          897 g          552 t
ORIGIN      
        1 gtcgggggct ctggacagga cctgttcggc ctcttcgcgc gacatccggc gattctcagc
       61 cttgagcatc atcgggacca gcgttaagtc cccgaccatc gcgagcccgg tgacctcgtc
      121 gtgagagagg acccacgaga cggcggcgtc tatatactcc tgcctgtcga agggttcgta
      181 ccaggtggtg tacctctggt cgcccgtcgg atcgccctcc ggccagttac ggcgcgagat
      241 ggtcttgatg atcatgagcc cagcatcctg gcgccgcacc tcttccacaa gcgcctcgta
      301 gtcggcgcgg tagccctcgt cagtagagag gatgtagttc cagggcgtaa gtaccgtctc
      361 gaatgggtaa cgccgaaggg cttcgagatg ggtcgctggg gcctcgttgc cgtgtcctgt
      421 gatgccgatg gccccgatga ggccctcttc cttcgcctcg atggcggcct ccagggagcc
      481 gccggggcct gtggcgcggt cgaggtcttc gatgtcgcct acagagtgga gttgaaggag
      541 gtcgacgttg tccacgcgca ggcgttcgag ggagtcctgg atctggcgct tcgccgcgtc
      601 tttctcgcgc agcccggtct tggtggagag aaagatgcgg tccctgatct caggcatcca
      661 gcgcccgtat ttcagctctg aatctccata gtcagccgcc gtatcgaagt ggtctacgcc
      721 ggcgtcgagc gcctggtcaa tggccacgtc cgagtcttct tcagtggctt ccgcaagggc
      781 ggccccgccg aagataagca cggagttctc gcggtcgaga cgtcccaaac gacggtgttc
      841 catgcctcgc cctttcctcg cttaccctat tctctttccc aatgcactct cagcgtaacc
      901 acctcgaaag gccgcaccct gaggcgaacg gcacctctat gaacctcgac gggcttttcg
      961 gcttcttcga ggaggttgac gcgctccacc ctgtcgacgg ggcgagcgaa gcgcaccgta
     1021 cattcaccgc gggcgccatg gggctcgtag aggcgcagga tcacagagtc cccgtcctcc
     1081 gcgagcttga ggctcccgag cgcaagctcc ggtccttcgg cctccacgag agagaatccc
     1141 cgcggcaggg cgtcctcctg cgacctaacg ggaaagagag gcgcgttgag ggcgaaggct
     1201 tcgcgtgcga ctcccgccgt ggtccaatcg ccggggtggg gaaagaggga gtaggtaaag
     1261 cggtgcccgc cctcgtcggc gaacgggtcg gggtagagcg ggctgcggag caggctgatg
     1321 cctaccacgt tcccctttgc gctgtggccg tacttgccgt cgttgaggag tgccacgccg
     1381 tagcctggct cggagatgtc acagaagcgg tgggccgaga cctcgaaacg ggtggcatcc
     1441 caaggtgtgt tgtgatgcgt cggccgtcgc agggccccga acatcgtctc gctggtagtc
     1501 tcgtgggagc ggatggcgag cgggaagagt gtcctgagta ggaccctccg ctcgcgccag
     1561 tcgacgtagg cctctacgtc cagccgtcgc gagccggaga ggagcctgta ggtcttcgca
     1621 atgatcgagt ctcgccaact ccggatcaca cccacggagg ccctgagtgg gccgctctcc
     1681 gtgacctcga tgctctctac agaggagatc tcctccccct ctttctcgta cccctcgtcc
     1741 acgtcccacg cctcccagtt gcggggcttg tccgtgtagg cccagagctg gttggcgcgt
     1801 ccggccagaa cctcacggcc ggcctccctg tcgtagacac cgtggagcgt gccgtcctcc
     1861 cctacctcca cgcgcaagag tccgttgtca agaacggcgc gcccgcctgc cacctcagca
     1921 ccgacatcct gaccctcgcg ccgggccggc gcggattcat gcctggccgt tagcgtactc
     1981 cagccgattc ccggcacgct gagatccggg gcgtgcacga gcaactctcc gccctcggtc
     2041 acctgggtgg gcagtggcct gccatccgcg tctgcgacgc ccgaggtgcc ttccggcttg
     2101 ctctcagcat cgagcagcac ggagaggggg cgtggattca gtgagacgtt ggcgactagg
     2161 agcgcaggct ctgcttccgg tggggggctc agggcactat cgcgcagctt tcgcgctgtc
     2221 ctgacgacgt ccgccagctc cttgtgggtt acgtcgtaga cctcccctat cgaggttcca
     2281 ggcagtatgt catggaactg gttgaggagg agcgtcttcc aggctgcttc gagctcttcg
     2341 cgctggtact cgccgccgtg gagcgcggcc agcgtggcga gggcttccgc ctcgagcaga
     2401 cggtgctcgg cctcacggtt gagcttcttg accctggcct gggtggtcag ggttccccta
     2461 tgcaactcga ggtagagctc gccggtccat cttggtagtc cctctttcgg gagactttca
     2521 aagaactcct cgacgtgagc catgcggagg cgtggcatcg cggggaagct cttcatgcgg
     2581 gcgtagtttt cgagcatctg ctcgctcggt ccgccgccgc cgtcgcccca tccgaaggag
     2641 aagaggctct cgggatggtg gcgcttgccc tcgaagttgc gccaggtgcc gtagatgtcg
     2701 tgtggtgtaa tattgccatt gtagtcctgg ccggggttct cgaagtcgtg ggcgatt
//
LOCUS       DATMJA010000270         3456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_332491, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000270 DATMJA010000000
VERSION     DATMJA010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332491"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VNT12_06405"
     CDS             complement(<1..338)
                     /locus_tag="VNT12_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HWI45784.1"
                     /translation="MAWIDLYKPTQQEFESVAQELELPPRAIEGAIEPQQRPKLVRYG
                     DSLFVVLKTARYLDEAEKVEFSEIHVLVGKDFIVTVFYEEIPALGEVHRRLEGEPELL
                     RQGPQPILHE"
     gene            complement(1013..1360)
                     /locus_tag="VNT12_06410"
     CDS             complement(1013..1360)
                     /locus_tag="VNT12_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45785.1"
                     /translation="MSILFAAMYPNRTSALIMYGAFPKFNYWVPTTEALEHLLTKIEQ
                     GWGAAATLPMFAPSFVEDPQFRQWYARFERLGASPGAVKALMRMNSEMTRRARSLRGS
                     TTRYTRTYLPRCS"
     gene            1629..1820
                     /locus_tag="VNT12_06415"
     CDS             1629..1820
                     /locus_tag="VNT12_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45786.1"
                     /translation="MIGDEDVAAFRILSLWWKHPFYFPRQKPGVEAHHRTSSTGETIT
                     ILKDFERGAVRETVRVIDG"
     gene            complement(1817..2434)
                     /gene="nthA"
                     /locus_tag="VNT12_06420"
     CDS             complement(1817..2434)
                     /gene="nthA"
                     /locus_tag="VNT12_06420"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HWI45787.1"
                     /translation="MSDHTHEETGSTTNPYAARIRAIEALLLEKGVLTEPEVQDGIAY
                     MEARSPANGAKLVARAWVDPDFKELLLSDGKAAAKELGMDPEHPAEFVVVENTPEVHN
                     LIVCTLCSCYPRAILGRPPDWYKSFAYRSRAVREPRAVLREFGFEPPEGVEILVHDST
                     ADVRYMVLPMRPPGTEGMDEEELAGLVTRDSLVGAGVPRTSVRIG"
     gene            complement(2431..2721)
                     /locus_tag="VNT12_06425"
     CDS             complement(2431..2721)
                     /locus_tag="VNT12_06425"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HWI45788.1"
                     /translation="MTFEPGDRVRVLAAEKPGHLRTPEYLKGKTGWVESVIGTFPNPE
                     ELAYGLSGLPERPLYKVGFRQIDLWNDYTGPAEDFLYADIYEHWLEPERGTT"
     gene            complement(2718..3017)
                     /locus_tag="VNT12_06430"
     CDS             complement(2718..3017)
                     /locus_tag="VNT12_06430"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45789.1"
                     /translation="MGGVHDMGGSSAGPVDKGQHEVEDWERLADAINGALGAKGLQST
                     DQLRRAIESLEEYRNLSYYERWVAATEKLLVEKGVLTSDEIDERAAQIQRRWNEP"
     gene            complement(3028..>3456)
                     /locus_tag="VNT12_06435"
     CDS             complement(3028..>3456)
                     /locus_tag="VNT12_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45790.1"
                     /translation="NLFYPELTGFVQPLAGEFVADRELFSSIPFLTGYAVETGIMIDV
                     LKMVGLEAMAQVDLGTRQNRHQPLRDLSRMAYSVLRAVARRMRQEGRLNQARDPGMPD
                     SLFQLSDYLHAVATPDGLKLQEYVEELVERPPIKEVLRVG"
BASE COUNT          643 a         1054 c         1062 g          697 t
ORIGIN      
        1 atctcgtgca ggatcggttg tgggccttgg cgcaacaact caggctcccc ctccagccgg
       61 cggtgcacct cgcccaaagc cggaatctct tcgtagaata ccgtgacgat aaagtccttc
      121 cctaccaaaa catgtatctc gctgaactcc actttctctg cttcgtccag gtaacgagca
      181 gttttgagca cgacgaatag cgaatcgccg tagcgcacga gtttgggcct ctgctggggt
      241 tctatagcgc cctctatcgc cctggggggc aactctaact cctgcgcgac cgattcgaac
      301 tcctgttgtg ttggtttgta gaggtcgatc caggccactc catcgtctag tcgacgaagg
      361 gcctgatgcg tttcttgcaa agagctgggt tgttgtgcag cgcgtcgacc gtcgacatag
      421 atggcgttgt aggcaatcat agatcaataa tatcggcaaa gcgtgcctga gagtgttgag
      481 cgtcgccagg aacatatccc tgagagtaag ggctccgagc actaggcggc tcgcctctgg
      541 ccctattcac ccgagtgcgt agaagacgag ttttccgaag ttcgcctgta cggaggtctc
      601 ggaagttcag cttagggatg cggaacgcct cagcgtaagg agggtgatgt cgtcctcctg
      661 ctcccaaccc tctccaacga acgaataaag ctcctctagg agtgggtcca cttgcgatcc
      721 ttccttgcca tgctccgcca tgagcgctcc caaccgcggg aagccaaaca tctggtagtg
      781 tggattatgg gcctctacca acccatcgcc ttagaacagg acgctgtcct cctcgctcag
      841 agaggcttct ttctcctcat agctcatctc tcgggcataa gccccaaggg cgtccccgta
      901 gcccgcagat cgttagtgcc ttcgccgtga tggtgcacgt agggcaggtc atgtccggcg
      961 ttggcgtatc gaagccgacc tgcggccgga tcgaggatgg catagaaaca ggtcacgaac
     1021 atctcggaag gtatgtccgg gtatagcgcg ttgttgaccc gcgcaaggac ctcgcccggc
     1081 gagtcatctc gctgttcata cgcatcagag ccttcaccgc gcctgggctg gcgcccaagc
     1141 gctcgaagcg agcataccac tgcctgaact gcggatcctc aacgaagctc ggcgcaaaca
     1201 tgggcaaggt agcggcggca ccccagcctt gctcgatctt cgtcagtaga tgctccaacg
     1261 cttccgtcgt cggaacccag tagttgaact tcggaaacgc accatacatg atcagggcgg
     1321 acgtccggtt ggggtacatg gcggcgaaga ggatgctcat tgggcccctc cgagatgcca
     1381 aacatggcgg cttgctcgct tactacggca tccatcaccg ctcgcacgtc gtccatgcga
     1441 tcttccagcg tcgcgatgcc agccacccgg tccgagagcc ccgtctcccg cttgtccaag
     1501 aggataagcc gagagaagga agcgaggcgc tgcacaaacc ggacaacagg ggatgcttcc
     1561 cagtaatgct cgaggtgtga cacaaagccg ggcacgaaca cgaggtcgag aggagcatcg
     1621 ccgagcactt gataggcgat gaagacgtcg ccgcttttcg catactgagt ctctggtgga
     1681 aacacccctt ctactttccg cgccagaaac caggggtgga ggcccaccac cgtacgagca
     1741 gcacgggaga gaccataacg atcctcaagg acttcgagcg cggggccgtt cgagagactg
     1801 tacgggtgat agatggctaa ccgatgcgca ctgacgttct cgggacgccg gctcccacca
     1861 gagagtcgcg cgtgacgagc ccggcgagct cttcttcgtc catgccctca gtgccgggag
     1921 ggcgcatggg gaggaccatg tagcggacgt ccgcggtgct gtcgtggacg agtatctcga
     1981 cgccctcggg aggttcgaag ccgaactcgc gcaatacggc acgcggttcc ctgacggcgc
     2041 gcgagcggta agcgaagctc ttgtaccagt ctggtgggcg gccgaggatg gcccgcggat
     2101 agcaagagca cagggtgcag acgatcaggt tgtggacttc tggcgtgttc tccaccacta
     2161 cgaactcggc ggggtgctcg gggtccatgc ccagctcctt agctgcggcc ttaccatcgg
     2221 acagtagcaa ctccttgaaa tccgggtcta cccaggcgcg ggccactagc ttcgcgccgt
     2281 tggcagggga gcgggcttcc atgtaggcga taccgtcctg cacctccggc tccgtgagaa
     2341 cgcctttctc gagcagcagc gcctctatgg cccggatgcg cgcggcgtaa gggttcgtcg
     2401 tgcttcccgt ctcctcatgc gtatggtcgc tcatgtggtt cccctttcag gctccagcca
     2461 gtgctcgtag atgtcggcgt agaggaagtc ttcagcgggg cccgtgtagt cgttccagag
     2521 gtcgatttgt ctgaagccga ccttgtaaag gggccgttct ggcaggcccg agaggccgta
     2581 ggcgagttct tccgggttgg ggaacgtgcc gatcaccgac tcgacccatc cggtcttccc
     2641 tttaaggtat tctggcgtgc gtaggtggcc aggcttttcg gcggcaagca ccctgacgcg
     2701 gtcgccgggc tcgaacgtca cggttcgttc caacggcgtt ggatctgggc cgccctctcg
     2761 tctatctcgt cgctggtgag gacgcccttc tcgacgagga gcttctcggt cgccgccacc
     2821 caacgttcgt agtaggagag attccggtac tcttccaggc tctctatggc cctgcggagc
     2881 tggtctgtgc tctggagtcc cttcgccccg agcgccccgt ttatagcgtc ggcgagacgc
     2941 tcccagtcct cgacctcgtg ctggcccttg tctacggggc ctgcggagga accgcccatg
     3001 tcgtggaccc cgcccaaggc attgctccta tccgaccctc aggacttcct tgatcggggg
     3061 tcgctcgaca agctcctcga cgtactcctg gagcttgagg ccgtccgggg tggcgaccgc
     3121 gtgcaggtaa tccgagagct ggaagagcga gtccggcatg ccagggtcgc gcgcctggtt
     3181 caggcgtccc tcctggcgca tgcgcctggc gaccgcgcga aggacagagt aggccatgcg
     3241 ggagagatct cgcaggggct gatgcctgtt ctggcgggta ccaaggtcga cctgcgccat
     3301 tgcctccagc ccgaccatct tgaggacgtc tatcatgatc ccggtctcga ccgcatagcc
     3361 ggtgaggaac gggatggagg agaagagctc cctgtcggcg acgaactccc ctgcgagggg
     3421 ttgcacgaag cctgtgagct cggggtagaa gaggtt
//
LOCUS       DATMJA010000271         2707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_155145, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000271 DATMJA010000000
VERSION     DATMJA010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155145"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VNT12_06440"
     CDS             <1..234
                     /locus_tag="VNT12_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45791.1"
                     /translation="LAAIFMATAAASAEWALYPFAFAWAAVDDPAGYADRFGSVGRPS
                     YVDWTTYDILGVGISSALAQGLRMMANVNPGGR"
     gene            complement(215..484)
                     /locus_tag="VNT12_06445"
     CDS             complement(215..484)
                     /locus_tag="VNT12_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45792.1"
                     /translation="MRTITIRYTAFRECPNCEGRGDDGASWRPTECRRCRGRGRLVSG
                     VEVEDSVDGVLPRSRYEFNALGEVYLLDFDEEEEFEVVRPSDPRG"
     gene            complement(505..1755)
                     /locus_tag="VNT12_06450"
     CDS             complement(505..1755)
                     /locus_tag="VNT12_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWI45793.1"
                     /translation="MIGESLVKDPSLAPEGHRKIDWVAQHAPVLNKIAGEQLADGSLR
                     GRKVAMTIHLEAKTAYLALLLSEAGADVTVSGSNPLSTQDDVCAALAERGVRVFATHD
                     PSDEDFEGYLHKTLETGPDLIVDDGAELVGRLVEHYPDLIGNVLGASEETTTGILKLR
                     AMTAEEVLPFPVLAVNDAKMKHLFDNRYGTGHSSIVALLSNTNLFISGKAVVVMGFGW
                     VGRGLAKYADGMGARVIVCEPDPVKLLEAYSEGYEAMNSLQAAEVGDVFLTGTGNLKV
                     LGIEHFARMKDGALLANAGHYDHEFDLAALRETAARSREVRRNITEYELPDGRRLHVL
                     ARGRLVNIAAADGHPVEIMDLTFAVQALAAHHLASHAADLTPGIQPIPETIDDLVARA
                     KLAALGVEPEILTEEQISYQQSWR"
     gene            1913..2212
                     /locus_tag="VNT12_06455"
     CDS             1913..2212
                     /locus_tag="VNT12_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45794.1"
                     /translation="MSYVTIQVIWLLCIFVTPPALKLYPSKLWALPFCMVTVANGLHM
                     IFFRYEYNAVLRRAIHILPYAEFLTPLREEPRYFLPLGTAYTLFGVASIVLLMAA"
     gene            complement(2199..>2707)
                     /locus_tag="VNT12_06460"
     CDS             complement(2199..>2707)
                     /locus_tag="VNT12_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWI45795.1"
                     /translation="RVPTLGEVLEEFPGVAVNIDMKAGTPGIEETVFGVLREANALGR
                     VLVVSIPHATLKLFRKVSGGHVSTGASRWEIGVFYISSRVRLERLVRPAYDALQVPLR
                     HRGIPVVTPRFVRAAHARGVRVDAWTINQADEMRRLLDLGVDVIMTDRPGTLAEVLEN
                     RPPTFTPP"
BASE COUNT          465 a          890 c          819 g          533 t
ORIGIN      
        1 cttgccgcca ttttcatggc cacggcggcc gcatccgctg agtgggcact ctatcctttc
       61 gccttcgcgt gggcggccgt cgacgacccg gcaggttacg ccgacaggtt cgggagcgtc
      121 gggcgtccct cttacgtcga ctggaccacc tacgatattc tcggggtcgg gatctcctct
      181 gcactggcgc aaggcctgcg aatgatggcc aacgtcaacc ccgggggtcg ctaggacgca
      241 cgacctcgaa ctcttcctct tcgtcgaagt cgagcaggta gacctcgccc agggcgttga
      301 actcgtagcg cgagcgtggg agaacaccat cgacggagtc ctcgacctcg acgccggaca
      361 cgagcctgcc ccttcctctg caccgcctgc actccgttgg gcgccaggag gcgccgtcgt
      421 ccccccgccc ctcgcagttg gggcattccc ggaaggccgt gtaccggatc gtaatggtcc
      481 tcatgcttcg tcccccgtgc cgtcctacct ccagctctgc tggtaggaga tctgttcttc
      541 tgtgaggatc tcgggctcta cgccgagggc ggccagcttg gcgcgggcga cgaggtcgtc
      601 tatcgtctcg gggatgggtt ggatgcccgg cgtcaggtcg gctgcatggc tggcgaggtg
      661 gtgtgccgcg agcgcctgca cagcgaaggt gaggtccatg atctccaccg gatgcccgtc
      721 tgcggctgct atgttgacca gtcgcccacg cgccaggacg tgcagccgcc ttccgtcagg
      781 gagctcgtac tcggtaatgt tgcgcctgac ctcgcgtgag cgtgccgccg tctcgcgcag
      841 ggcggcaaga tcgaactcgt gatcgtagtg cccggcgttc gcgagaagtg caccgtcctt
      901 catccgcgcg aagtgctcaa tcccgagcac cttgaggttg cccgtgcccg tcagaaacac
      961 gtccccgacc tctgcagcct ggagcgagtt catcgcctcg taaccttcgg agtatgcctc
     1021 gagcagcttc acagggtcgg gctcgcacac gataacccgg gcgcccatgc cgtcggcgta
     1081 cttcgcgagg ccgcgcccca cccagccgaa gcccatcacc acgaccgcct tgcccgagat
     1141 gaagaggttg gtattcgaga gcaaagcgac gatggaggag tggcccgtac cgtagcggtt
     1201 gtcgaagagg tgcttcatct tggcgtcgtt gacggccagc acagggaagg gtagtacctc
     1261 ctcggccgtc atggctctta gcttaagtat gcctgtggtg gtttcctcgg aggctccaag
     1321 tacgttgcca atgaggtcgg ggtagtgttc gaccagacgt ccgacgagct cggcgccgtc
     1381 gtcgacgatc aggtcgggcc cggtctcgag tgttttgtgc aggtagccct cgaagtcctc
     1441 gtcggaaggg tcgtgggtgg cgaagaccct cacgccccgc tcggccagag ccgcgcaaac
     1501 gtcgtcctgg gtggagaggg gattgctccc ggagacggtg acatcagccc cggcttcaga
     1561 caggagcaag gccagatacg ccgtcttcgc ttccaggtga atggtcatcg cgaccttgcg
     1621 gccgcgcagc gagccatcgg cgagttgctc gcccgcgatc ttgttcaaga ccggcgcgtg
     1681 ctgggcgacc cagtcgatct tgcggtgtcc ctcaggggcg agggaagggt ctttgacaag
     1741 cgattcaccg atcatgtttc tcctactcta tataccgttc tacagccgga gtcaacctca
     1801 tctacatacc gaccagtgta caatcacggc aaactgaatg ctctcgtgtc gatcactaca
     1861 ttagaatctc accatcgacg agaagcacag atacaggatc acggcagcct gggtgagcta
     1921 cgtgaccatc caggtgatct ggctgctctg catcttcgtc acgccccctg ccttgaagct
     1981 ctatccttcg aagctctggg ccctgccatt ttgcatggtc acggtcgcca acgggctgca
     2041 catgatcttc ttccgctacg agtataacgc cgtcctcagg cgcgcgatac acatcctccc
     2101 ttacgccgag ttcctcacac ccttgcggga agagcccagg tactttctgc cgctcggcac
     2161 cgcctacacg ctctttggcg tggcttccat cgtgctgctc atggcggcgt gaaagtcggg
     2221 ggccgattct ctagcacttc agccagcgtg cctggccggt cggtcatgat cacgtcgacc
     2281 ccaaggtcga gcagccggcg catctcgtcc gcctggttta tggtccacgc gtccaccctc
     2341 acgccccttg catgcgccgc tcggacgaag cgcggggtca ctaccggtat tcctctgtgc
     2401 ctgaggggaa cctgcagcgc gtcgtaggcc gggcgaacga gacgctccag gcggaccctg
     2461 ctcgaaatgt agaaaacccc aatctcccac ctcgaagccc ccgtggagac gtggccgcct
     2521 gagaccttac ggaacagctt caaggtagcg tgggggattg agactacgag gacacgcccc
     2581 aaggcgttcg cctcgcgcaa gaccccgaag accgtctcct ctataccagg cgtaccggcc
     2641 ttcatatcga tgttgaccgc gacgccgggg aactcctcga ggacctcccc gagcgtcggc
     2701 acccgaa
//
LOCUS       DATMJA010000272         2828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_391916, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000272 DATMJA010000000
VERSION     DATMJA010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391916"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..512
                     /locus_tag="VNT12_06465"
     CDS             66..512
                     /locus_tag="VNT12_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45796.1"
                     /translation="MAVEGKVAKILGNNEIVINRGRGVGVRQGMLFEIFAPEGEEVWD
                     PDTGETLGTVEDVKAKAEVTEVKDRLAVARLQNVQDTPFGAVNIGEMQENLQRMFGQM
                     FGEGVQIRGFGATSGDDPDLESMLGAPLQDLSQVQVGDAVREIKLR"
     gene            534..1223
                     /locus_tag="VNT12_06470"
     CDS             534..1223
                     /locus_tag="VNT12_06470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWI45797.1"
                     /translation="MNDLSRLPVTFGLIAACVAVYVAVALAGQGYGYPLNVGLVTQPP
                     EVLAQGALVPAAVADGRVWLLVSSMFLHSGFIHLALNMLSLYFLGSFVEQAFGKSRFL
                     ALYLSSGLAGGIAYLYFGAFDRPAVGASGAIFGLLGGVLGYSVRRGTFSWQNPLIRQL
                     LILTALNLYFGFSVPNISNTAHIGGLVGGAAFGWLTAPSVYSSKTLRAATPTVILFGA
                     ELILLALWLLL"
     gene            complement(1429..2157)
                     /locus_tag="VNT12_06475"
     CDS             complement(1429..2157)
                     /locus_tag="VNT12_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWI45798.1"
                     /translation="MNAILMASLAAGGFAAASLLALLGGELGARGRSYAAAFAAGILL
                     ALAFVDLFPEGLELGGEAAVAGFVGGFALLFMTEAFTRAHTHHLPEEHVHKHALGPFI
                     LGLAVHNLADGFVLGVGAKAGAVTSWLVGLGVLVHQIPVGISLAAVLIAARVGRAGVM
                     RAAVLLGLAIPAAAALTVALPAPGAGTLGLMTGAAGGVLAYIGAAHLLPEAQAEDTNR
                     LAGIMFVATFVITAAGLMAFQHFS"
     gene            2249..>2828
                     /locus_tag="VNT12_06480"
     CDS             2249..>2828
                     /locus_tag="VNT12_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45799.1"
                     /translation="MRTDICKIAGGFLRVKAARLGDCGSADKFCEIFVNSLELVASGN
                     HKRLALFARFRSILSGRDWVYLSGLLVPLVVYNLVLKGIRIDSQEDLPWGFAAFALMR
                     SDLLFNLGYVFLWIGLFALTRQSRLRWAVVVLFHTVTTVVFATTTSAHVYFQETGSTL
                     SLSVITYSLSSLGEITDVIGSVTSLAIWAWVLV"
BASE COUNT          509 a          898 c          874 g          547 t
ORIGIN      
        1 gcgaccctcg ctaggagaga tataatctcg taagaccatc aggatgatcg gaaggagcca
       61 accgcatggc ggtcgaaggc aaggtagcga agattctagg aaacaacgag atcgttataa
      121 accgcggaag aggggtgggc gtccgtcagg gcatgctctt cgagatcttc gctcctgagg
      181 gcgaggaggt ctgggaccct gatacgggcg agacgctcgg caccgtcgag gacgtaaagg
      241 cgaaggccga ggttacagag gtcaaggaca ggctcgccgt tgcccgtcta cagaacgtgc
      301 aggatacgcc gttcggggcc gtcaacatcg gagagatgca ggagaatctg cagcgtatgt
      361 ttgggcagat gttcggcgaa ggcgtccaga tccgcggctt cggcgctacg tcgggcgacg
      421 accccgacct cgagtcaatg ctcggcgccc ccctgcaaga cctttcacag gtacaggtcg
      481 gggacgccgt gcgcgagatt aaactccgct agacccaaag taagtatcag gcattgaacg
      541 atctctcccg cctaccggtg acgttcggcc tcatagccgc ctgtgtggcc gtatacgtcg
      601 ccgtcgcgct cgcgggtcag ggctacggtt acccgctgaa cgtcgggctg gtgacacagc
      661 cccccgaggt gctcgcacag ggcgccctcg taccagccgc cgtcgccgat ggcagagtgt
      721 ggctcttggt gtcgagcatg ttcctgcact cggggttcat ccacctggcg ctgaacatgc
      781 tctctttata cttcctcggc tcgttcgttg agcaagcgtt cggaaagagc cgtttcctcg
      841 cgctgtacct atcgagcggg ctcgccgggg gcatcgccta cctctacttc ggcgccttcg
      901 acagacccgc cgtaggggct tccggcgcga tcttcggtct cctcggtggc gtgctcggtt
      961 actctgtgcg ccgcggcacc ttctcctggc agaatcccct gatccgccag ctcctgatcc
     1021 tgactgccct gaacctctac ttcggattct ccgtccccaa tatcagcaac acggcgcata
     1081 tcggcggtct cgtcggcggg gccgcattcg gatggctcac ggcgcccagc gtctactcca
     1141 gcaagacgct ccgcgcggcg acaccgaccg ttatcctgtt cggcgcggaa ctgatcctgc
     1201 tcgccctgtg gctgctcttg tagctggtca gcacggcggc agcgccgcct tctcagcacg
     1261 ctcaggcctt tgaggccttc gcttctcagc aggtcggctt tccccgtgct ggcccagctc
     1321 ggcagcgcac cagaagcgca gtctgtcgca ctcgggttta cgctcatggg gaggtggtgg
     1381 gaccactagc tgaaatgctg acaagccgcc cgcagggcgg cgtgctgact agctgaaatg
     1441 ctgaaatgcc atgagtcccg cggcggtgat gacgaacgtg gccacgaaca tgatgccggc
     1501 caacctgttc gtatcctcgg cctgggcctc ggggagcagg tgtgcagcgc cgatgtatgc
     1561 gagcaccccg cctgccgcac cggtcatgag acctagagtg cctgctcctg gcgcggggag
     1621 ggccacggtc aacgccgctg cggccgggat cgccagtccg agtaagacag ccgctctcat
     1681 aacccctgcc cttccgacac gcgccgcgat caggacggcg gcgagagaga tcccaacggg
     1741 tatctggtga actagaaccc cgagccctac gagccaggag gtgacggctc cagccttggc
     1801 gcctacgccg agaacgaatc cgtcggcgag gttgtggacg gccagcccta ggatgaaagg
     1861 cccgagcgcg tgtttgtgga cgtgctcctc gggcaggtgg tgcgtgtggg ccctggtgaa
     1921 cgcctctgtc atgaacagga gggcaaaccc accaacgaac ccggcgaccg cggcctctcc
     1981 accgagttcg agaccctcgg ggaagaggtc gacgaacgcg agcgcgagga gaatccccgc
     2041 ggcgaaggcg gctgcgtagg accggcccct ggctcccaac tctccaccga gtagggcgag
     2101 aaggctcgcc gccgcaaagc cccctgcggc cagcgaagcc attaggatcg cgttcatagc
     2161 cgccacccgc catctgccgt ggcgacatgc aaccctacct tacgcccctc tacctgcagt
     2221 cggagcacgt cccgtaaacc tccaggctgt gcgcacggac atctgcaaga tagcaggggg
     2281 attcttgcgc gtcaaagcag cccggttggg agattgcggg tcggctgaca agttctgcga
     2341 aatctttgta aactctctcg aactcgtggc ttccggaaat cacaagcggc tggcgctctt
     2401 tgcccgcttc cgctccattc tgagcggtcg cgactgggtc tacctttccg gattgctggt
     2461 tcctcttgtg gtctacaatc tggtgttgaa gggaatccgg atagactctc aggaagacct
     2521 tccgtggggg ttcgccgcct tcgccttgat gcgttcggac ctgctcttca acctcgggta
     2581 tgtgttcctc tggattgggc tattcgccct gactaggcag agtcgtctcc gctgggctgt
     2641 ggtagtgctc ttccatacgg tcacaacagt cgtctttgcg acgacgacct cggcccacgt
     2701 atacttccag gagaccggct cgacgctcag cctcagcgtc atcacgtact cactcagctc
     2761 actgggagag atcacagacg taatcggcag cgtaacttcg ctggctatct gggcctgggt
     2821 tctggtca
//
LOCUS       DATMJA010000273         3167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_95574, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000273 DATMJA010000000
VERSION     DATMJA010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95574"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2460
                     /locus_tag="VNT12_06485"
     CDS             <1..2460
                     /locus_tag="VNT12_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI45800.1"
                     /translation="SDPIPKDGGTEAGASEGIADEPLLDRVPPEDLPEELLLRRLGRL
                     DTRRALAEICRNVPALEEILLPRGIQADALTRKSGGGAPLLRGLARRISHDAAAWGAF
                     RTAIQEQIPPETFEALEGFSPEKLEELKQAHTTEGLLLAALSAEEEPEEGVVGTLVAA
                     WREERGEAEKRASEDARVRDLEAELERLKQDNERLSFGARAAKERAAALAEEVDVLTG
                     ERTDTSELVRKAEERAASALDLRGKLDERIEDLERRNSQLEKALDGERSAYSRAAERV
                     EELHEELGRVVEERDRIKNALQDARFTDRGFGELLVRSIKNEVAALPASLESTAQAAR
                     LMEFMGKVLQAHADLRSPGEQGRRTAEEDPDDKDPSLAGRQKERPAGSATLPRVGGYA
                     KPRPTLSFRALGGAGEVGGSSHLLDFGKTRILVDAGIKPDGRGPVTPDFGGLDGLDAA
                     VVTHAHLDHCGALPLLVRDRPKMPIYCTPPSAKLIVSALNDHAAMGGGLPGGAPIHEV
                     RKRLVPVPFGKPLDVGGAKVTLTESGHILGAASVLFSSGEATVFHTGDICMEDHFSIP
                     SARLPEVRDIDLLIMEATLADQKPQPFSESIRTMVEVINDTTLERGGTVLIPTYALGQ
                     AQEIILGIKHYGREYGLDREVFIYVDGSVVTTSERLYAEQLGYMKPYLQHSDPREIFF
                     SENIRAVANDDGARERILSNPCAIIASPVTMQGGASAFYRKRLEKSFENAVILPSNAA
                     SSYGTHRAEGEEEQWRVERVSFAAHCTQDELLGITEKLSPRQIILIHGSRRRISDLAF
                     RLAPNHKIHTPTVGETVRTVL"
     gene            complement(2542..2880)
                     /locus_tag="VNT12_06490"
     CDS             complement(2542..2880)
                     /locus_tag="VNT12_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45801.1"
                     /translation="MHEELSQYNLEPSGYSGRILGWSAEAGEAPCQTFAVYRDGVWKT
                     RGLFVTRACVRVDDPDHVLKGFCLRIMGRLIDLASLTGGAGRRWTDEPYALRDLLEGR
                     RAPTALRWPR"
     gene            2875..3078
                     /locus_tag="VNT12_06495"
     CDS             2875..3078
                     /locus_tag="VNT12_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45802.1"
                     /translation="MHEILLGLGRRAWHDRERDAGLTAWSSHGPQVERRTSVGGLYLE
                     SGLPPQYRRILEPREETLGEVAL"
BASE COUNT          628 a         1040 c         1034 g          465 t
ORIGIN      
        1 tcagatccca tacccaaaga cggaggaact gaggccggag cctcagaggg aatcgccgac
       61 gaacccctgc tcgacagggt gccgcccgag gatctacccg aggagttgct cctgcggcgt
      121 ctcgggaggc tcgacaccag gcgcgcgctc gccgagatct gcaggaacgt gcctgccctc
      181 gaagagatcc tacttccgag gggtatacag gccgacgccc tgacgcgcaa atcaggcggt
      241 ggcgcgccgc tcctgcgggg cctcgcgcgc agaatctccc acgacgcggc agcctggggc
      301 gcgttccgca ccgctatcca ggagcagatc ccacccgaga ccttcgaagc cctggaaggt
      361 ttctccccgg agaaacttga ggaactcaag caggcccaca ccaccgaggg tttgctgctg
      421 gccgccctca gcgcggaaga ggagcccgaa gagggcgtcg tcgggacgct ggtggccgcc
      481 tggcgggagg agagaggcga agccgagaaa cgggcttccg aagatgcgcg tgtcagggat
      541 ctcgaagcag aattggagcg cttgaagcag gacaacgagc ggctctcttt cggagccagg
      601 gccgccaaag agagggccgc cgccctcgcc gaagaggtcg atgtccttac cggcgaacgc
      661 actgacacct ccgagctggt caggaaggca gaggagaggg cggcctcggc gctggacctg
      721 cggggcaagc tcgacgagcg catcgaggac ctcgaaaggc gcaactccca gctcgagaag
      781 gcgctcgatg gcgagcgctc ggcctattcc agggcggccg aacgcgtcga ggagctgcac
      841 gaggagctag ggcgcgtggt cgaggagcgt gacaggataa agaacgccct gcaggacgcc
      901 cgctttaccg acagaggctt tggtgagctc ctggtgcggt ctataaagaa cgaggtcgcg
      961 gccctgcccg cttcgctcga aagcacagcg caggccgccc gcctgatgga gttcatgggc
     1021 aaggtgctac aggcgcacgc cgacctgcgc agccccgggg aacagggccg caggaccgcg
     1081 gaggaagacc cagacgacaa ggacccttct ctagcgggcc ggcagaagga aaggccggcg
     1141 ggtagcgcca cgctcccaag ggtcggcggg tacgccaagc cgaggccgac gctatccttc
     1201 cgcgccctgg gaggggctgg agaggtgggt ggcagcagcc acctcctcga cttcggcaag
     1261 acgaggattc tggtcgacgc ggggatcaaa cccgacggac gcggccccgt aacgcccgac
     1321 ttcggcgggc ttgatgggct cgacgcggcc gttgtaacgc acgcgcacct ggaccactgc
     1381 ggggcgctgc ccctgctggt gcgagacagg cccaagatgc cgatctactg tacgccccct
     1441 tcggcgaagc tcatagtcag cgcgctcaac gaccacgcgg cgatgggcgg cgggctgcca
     1501 ggcggcgcac ccatccatga ggtccgaaag cggctggtcc ccgtaccctt cggcaaaccg
     1561 ctagatgtcg ggggggcgaa ggtcaccctg accgagagcg gccatatcct gggggctgcg
     1621 agcgtcctct tctcatcagg agaagcgaca gtgttccaca ccggcgatat ctgcatggaa
     1681 gaccacttct caatcccctc ggcccgactt cccgaggtca gggatataga cctcctcatc
     1741 atggaggcga cgctggccga ccagaagcca caaccattct ccgagtcgat caggacgatg
     1801 gtcgaggtca taaacgacac gacgctggag cggggcggaa cggtcttgat ccctacctac
     1861 gccctcgggc aggcgcagga gatcatcctc ggcatcaagc actacggtag ggagtacggc
     1921 ctggacagag aagtcttcat ctacgtcgac ggttccgtgg tgacgacctc ggagaggctc
     1981 tacgccgagc agctcgggta tatgaagccc tacctccagc acagcgaccc gcgtgagatc
     2041 ttcttctccg agaacataag ggccgtcgcc aacgacgacg gggcgcgcga gcgtattctt
     2101 tcgaacccct gcgccatcat cgcctcgcca gtaacgatgc agggcggggc cagcgctttc
     2161 taccgcaagc gactggagaa gagctttgag aacgccgtta tcctgccatc caacgccgcc
     2221 tcatcttacg gtacgcaccg cgccgagggc gaggaggagc agtggagggt cgagcgggtc
     2281 agcttcgccg cgcactgcac ccaggacgag ttgctcggca tcaccgagaa actaagcccg
     2341 cgtcagatca tcctgatcca cggctccagg cgcagaataa gcgacctggc cttcaggctc
     2401 gccccgaacc acaagatcca caccccgacc gtcggcgaga ccgtccgcac cgtgctctag
     2461 atcgattcac agaagcccaa agcttctgtg aatcacgccg tcagtctgtc gtcggggtga
     2521 ggctgcgaaa gctatgatga gttatctcgg ccagcgcagt gccgtcgggg cccgccggcc
     2581 ttcgaggagg tccctgagcg cgtagggttc gtcggtccag cgtctgccag cgccccctgt
     2641 gagagaggca aggtcgatca ggcgtcccat aatcctaagg cagaagcctt tcaacacgtg
     2701 gtcgggatca tcgacccgca cgcacgcacg ggtcacgaag agccccctgg tcttccacac
     2761 gccgtcgcgg tacacggcga aggtctggca gggggcctct ccggcctccg ccgaccagcc
     2821 gaggatacgg cccgagtagc cagaaggctc gaggttgtat tgactcaact cctcgtgcat
     2881 gagatcctcc tcggtctcgg ccgccgcgcc tggcacgacc gcgaacgaga cgccggcctc
     2941 acggcctggt ccagccacgg cccgcaggtc gagcgccgca ccagcgtagg cggtctctat
     3001 ctcgaatccg gcctgccgcc ccagtaccgc cgtatactcg agccccgcga agagacgctc
     3061 ggagaagtgg cgctatagcc tccgcttcct gagcgagctc gagtgatacg tcggcctctt
     3121 cgcggtagcg gcgcagacgg gcgagcgcgc tctcctcgaa gcccaag
//
LOCUS       DATMJA010000274         6263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_332697, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000274 DATMJA010000000
VERSION     DATMJA010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332697"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VNT12_06500"
     CDS             complement(<1..1266)
                     /locus_tag="VNT12_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI45803.1"
                     /translation="MLAQLRNVSLSFPDKKVLEDVSLTVYPGDRISLVGENGAGKTSI
                     FRMLKGRLAPDSGEISLSRGVRIGYLKQDFAGMKEDRKRTCMEVALEPFGLLIELEKR
                     IETLASELGEAGESERTSELLADLGEAQQRLEVSGGYEFRSRTPSTLTGLGLSEAFWA
                     RRVSELSAGERVRLALARLLLEDHDLILFDEPTNHLDVPAREWLEGHLAGMNAAYVVS
                     SHDRRFLDAVSRKVVHLDRGKLTLYSGDYTAFRQQLKQAEEEGWRRYEKSRKLAKKLQ
                     RQAQDYERWSEAGEKKKRGAADKGFVSHKAAKVMKRSLVARRRMEEALENARTEKPFE
                     KDAVKIEFGSSKGRSLMRAEELVVGYSKERPLTRELSFDLWSGDRFAVLGPNGCGKTA
                     LLRTALGEIPPLRGDVRLAPSSKVGYFDQD"
     gene            complement(1364..2446)
                     /gene="menC"
                     /locus_tag="VNT12_06505"
     CDS             complement(1364..2446)
                     /gene="menC"
                     /locus_tag="VNT12_06505"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HWI45804.1"
                     /translation="MKLARYDLYSYSLPFSRPLTLGGITLHHREGLLLKLSGDDGSEG
                     WGETAPLLGFSEESQGEAASQLRWLAGSMTGREVEEDWVDPYRAFGRELDRVAPSVRF
                     GFELAVWNLYAATSGRTLPEVVKSSPRNLVPVNGLLAGSPADVLVKARRMRDEGYRSI
                     KLKVGTRTVAEEVALVRELGEELGEGISLRLDANRAWGYEEAAEFVRGTAGVRFEYVE
                     EPLADPARLPGFVREFGVPVALDESLVGMEPEKVEETFAVAFVLKPTLLGGISRTLRV
                     AGRALHLGVTPVISSAYESGVGTAALVALAAGIGDRSVPAGLDPYRTVAEDVLETPLN
                     LPAPGLEVREAADASRRIDVRRLARL"
     gene            complement(2932..3783)
                     /gene="menB"
                     /locus_tag="VNT12_06510"
     CDS             complement(2932..3783)
                     /gene="menB"
                     /locus_tag="VNT12_06510"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HWI45805.1"
                     /translation="MTTIEWESAGEFEDIRYEKAEGIAKITINRPEVRNAFRPLTVIE
                     MQRALEDAREDLETGAIILTGEGPDAFCSGGDQKVRGDAGYLEDPDAPRRTPGGESIG
                     RFHVTDLHVQIRRCPKPVVAMVAGYAVGGGHVLHVVCDLTIAADNAKFGQVGPKVGSF
                     DGGFGASVLTSLVGPKKAKEMWFLCRMYSAEEALEMGLVNTVVPLAALEDETVSWCRE
                     MLARSPFALRLLKASFHAHEDGYAGIQQLAHDANLLFYGSEEAQEGRDAYKQKRKPDF
                     SRFPHRP"
     gene            complement(4262..4822)
                     /locus_tag="VNT12_06515"
     CDS             complement(4262..4822)
                     /locus_tag="VNT12_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWI45806.1"
                     /translation="MGKLIYSMITSLDGYVADKDGNFDWAAPDEEVHTFVNDLERPIG
                     TYLYGRRMYEVMSYWETAHALTDQPSFMQDFARIWRAADKIVYSKTLETASSARTRIE
                     RDFYPDTVRRMKSQAERDISVGGPDLAAQALKTGLVDECHLFVTPIVVGGGKRSLPND
                     VRLELELLDERRFEGGVVHLHYRIRT"
     gene            5189..5674
                     /locus_tag="VNT12_06520"
     CDS             5189..5674
                     /locus_tag="VNT12_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI45807.1"
                     /translation="MYPQTHRSLTKAREFMRRAYGRPVSLPDVAAQANLSPYHFLRVY
                     KRAYGETPHEFLTRLRIERAKTLLARGSHNVTETCFEVGFSSLGSFSVLFAYRVGLSP
                     SEYRRYARSTISVPSTVRALFVPSCFFTMLYGTQEERNIREAGRLEPMVSSGQSSETG
                     R"
     gene            5678..6094
                     /locus_tag="VNT12_06525"
     CDS             5678..6094
                     /locus_tag="VNT12_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI45808.1"
                     /translation="MIQRQSHSTIYVIDQEEALRFYRDILGFEVNTDMPMDDSGLRRL
                     TLNPKGQPDLELILAQVTSGPMFDAGTAEEMRALVERGAFGIGVFETDDIQSDYERLS
                     SQGVEFVSPPQERFYGTEAIVKDNSGNWFSLTQSKR"
BASE COUNT         1170 a         1998 c         1865 g         1230 t
ORIGIN      
        1 gtcctggtcg aagtaaccga ccttcgagga gggtgcgaga cgaacatcgc cgcgcagggg
       61 tgggatctca ccgagcgcgg tacgcaggag ggcggtcttt ccgcaaccgt tcggaccgag
      121 aacagcgaac ctgtcaccag accagagatc gaaggaaagc tctctggtca gcggacgttc
      181 cttcgagtaa ccgaccacga gttcctcggc gcgcatgagg ctgcggccct tcgaggaccc
      241 gaactctatc ttgactgcat ccttctcgaa cggcttctcc gttctggcgt tctcgagggc
      301 ctcctccatc ctgcgacggg cgacgagcga ccgcttcatg accttcgcgg ccttgtggct
      361 aacgaacccc ttgtccgccg cgccccgctt cttcttctcg ccggcctctg accacctctc
      421 gtagtcctgg gcctgccgct gcagcttctt cgccagcttc cggctcttct catacctgcg
      481 ccatccctcc tcctcagcct gtttcagttg ttgcctgaac gcggtgtagt cgccagagta
      541 gagggtgagt ttgcctcgat ctaggtggac gactttcctg gagacggcgt cgaggaagcg
      601 ccggtcgtgg gaggacacga cataagcggc attcatccct gcgaggtgcc cctccagcca
      661 ttcgcgggcg gggacatcga ggtggttggt gggctcgtcg aaaaggatga ggtcgtgatc
      721 ctctagcaac agcctcgcga gcgcgagccg gactctctca ccagcggaga gctcggagac
      781 cctgcgagcc cagaacgcct cggagaggcc aagccccgtc agagtagacg gtgtcctcga
      841 cctgaactcg tagccacccg agacctccaa gcgctgctgc gcctcgccca ggtcggccag
      901 tagctccgag gtgcgttcgc tttctcccgc ctcgccgagc tcggatgcga gggtctcgat
      961 tcgtttctcg agttcgatca ggagcccaaa aggctccagc gccacctcca tgcaggtacg
     1021 tttgcggtcc tccttcatgc cggcgaagtc ctgcttcagg tacccgatgc gtactcccct
     1081 ggagagcgag atctcgcccg aatctggcgc caggcgtccc ttcagcatgc ggaaaatgct
     1141 cgtcttcccc gcgccgttct cacctacgag ggagattcta tccccaggat agacggtgag
     1201 ggaaacgtct tcgagtacct tcttgtcggg gaacgagagc gagacgtttc tgagttgggc
     1261 gagcacgtaa agatcctttg caggacgagg ggaacatatt gcaaaggaag ctcgctacat
     1321 gcccgagagt atactccagc gccctcgaac tcgcacagag cagctagagt ctcgccagtc
     1381 tgcggacgtc tatcctgcgc gaggcgtccg ctgcctccct gacctccaag ccaggggccg
     1441 gcaggttcag cggcgtttcg agaacgtctt cggccacagt gcggtaggga tccagcccag
     1501 caggaacaga gcggtctccg ataccggccg ccagcgccac cagggccgcc gtcccgacgc
     1561 cgctctcgta tgccgaactg ataactggcg tcacgccgag gtggagcgcc cgccccgcca
     1621 cccgcaacgt ccgcgagata ccgccaagca gcgtcggctt gagcacgaag gccaccgcga
     1681 aggtctcctc caccttctcc ggctccatcc cgaccagcga ctcgtctagc gccacaggca
     1741 cgccgaactc cctgacgaac ccgggcagcc gcgcggggtc ggcgagtggc tcctcgacgt
     1801 actcgaagcg cacccccgcc gtgccccgca cgaactcagc cgcctcctcg tagccccacg
     1861 cccggttcgc gtcgagacgc agggagatgc cttcgcctag ctcctcaccc agctcgcgga
     1921 cgagggcaac ctcctcggcg acggtccttg tgccgacctt gagtttgatg ctcctgtaac
     1981 cctcgtccct catgcgccgc gccttcacga gtacatccgc gggagatccc gcgagcaacc
     2041 cgtttaccgg aaccaggttt ctgggagagg acttcaccac ctcaggcagc gtcctcccgg
     2101 aagttgccgc gtacaggttc cagacggcaa gctcgaaccc gaaacggacg gagggtgcga
     2161 cgcggtccag ctcacgacca aacgctctgt acgggtccac ccagtcctcc tcgacctcgc
     2221 ggcctgtcat cgagccggcg agccagcgta gctgcgaggc ggcttcgccc tgactctcct
     2281 cgctgaagcc cagaagcgga gctgtctcgc cccagccttc cgaaccatcg tcgccggaca
     2341 gcttcaggag gaggccttcg cgatgatgga gcgtaatgcc cccgagcgtc aacggcctgg
     2401 agaagggcag actgtaactg tagagatcgt agcgggctag cttcatcccg gcaactcctg
     2461 acagcatacc tgtgtcatct gatgtcatat tgacagttgt cgtggacggg cgcagaacgc
     2521 aagtaggttt ccgctctcga atagagtgac tggaagacag acctagctcg tcgcgtgtct
     2581 gggggcagac tttcgcgagc ggggcagaac gtcccgaacg tggttagact cctctcgtgg
     2641 tacacgtgag gcggcgacag acggatttgg cgagccagag ggcgtcgacc ctcgagctct
     2701 tctacgacct cgtgttcgcg atcacgcagg tctcctcacc tcctgctcga gcacctgacc
     2761 gtagtactgg tggcggtgat cggatctagc catctggcac cttcgagggc caaggcagaa
     2821 ggcgaagctc cgcctcgaag cagtttgcgg aagggctgag cctcccataa gtcagttgtc
     2881 aggctactag tatcaggaat tgcagtggat agctccttgc agagggcgcg gtcagggacg
     2941 gtggggaaac ctcgagaagt cgggtttgcg tttctgtttg taggcgtcac ggccttcctg
     3001 ggcctcctcg ctaccgtaga agaggaggtt agcgtcgtgg gcgagctgtt ggatgccggc
     3061 gtagccgtcc tcgtgggcat ggaagctcgc tttcagaaga cgtagcgcga aaggcgagcg
     3121 agccaacatc tcacgacacc agctgacggt ctcgtcttcg agtgcggcga gcggcacgac
     3181 cgtgttgacg agccccatct ctagcgcctc ttcggcggaa tacatcctgc aaaggaacca
     3241 catctcctta gccttctttg gcccgacgag gctggttagg accgacgcgc cgaatccgcc
     3301 gtcgaagctg ccgaccttag gaccgacctg cccgaacttc gcgttgtcag cggcgatggt
     3361 gaggtcgcat accacgtgca ggacgtggcc tccgccgacg gcgtatcccg ccaccatcgc
     3421 aacgacgggc ttggggcagc gccggatctg gacgtgcaga tccgtgacgt ggaagcgccc
     3481 gatagactct cctcccgggg tgcgacgggg cgcgtcaggg tcttcgaggt atccggcgtc
     3541 tcctcgtacc ttctggtccc caccagagca gaaagcgtca ggcccctcgc ccgtcaggat
     3601 gatggccccg gtctcgaggt cctcacgagc atcttcgaga gccctttgca tctcgatgac
     3661 cgtcagcgga cgaaaagcgt tccttacctc ggggcggttt atggtgatct tcgcgatccc
     3721 ctccgccttc tcgtacctga tgtcctcgaa ttctccggcc gattcccact ctatcgtcgt
     3781 catgcaatgg attatatggg accaagcacg tctaacaaaa ccgggtgtag ggtagggcgg
     3841 gggctcggcc accttcctct cggccgcctc cgatcccgaa aggagcccgc gtatctccgc
     3901 acggtcagat gcgtcgagtt tggctcccga agccgcaagc aacttggcca gcttgctatt
     3961 ttcgagtacc ttgaaacctg aaggcgatgg cccgttcgct ctgtgtcttg gttctcccac
     4021 gcccccaatg agaggaactt cctgggctct tcggtgctaa gccttacatt aatgatgccc
     4081 ccgcatgaaa gtgtaacgtt cccggcaaga gtacatgtcg cacctcccaa cacgccgatt
     4141 cgaccacgag cagcgttcaa atcaccgctt ggggagtgga aggcggtttc tcgtagagcc
     4201 ccggttctag tggcttaggc tattcggggc ttgagttggt tcgtgtgacg tcgcggaccc
     4261 cttaggttct gatgcggtag tggaggtgaa ccacgccgcc ttcgaaacgg cgttcgtcca
     4321 gcagttcgag ttccaggcgg acatcgttgg ggagggaccg tttgccgcct cccaccacga
     4381 tgggcgtgac gaacaggtgg cactcgtcta ccaacccggt cttgagcgcc tgggcggcga
     4441 ggtcgggccc gcccacgctg atgtcgcgtt ccgcttgcga tttcatccgt cgaacggtgt
     4501 cagggtagaa gtctcgctcg atccgggtcc tggcgctgga tgccgtctca agcgtcttgg
     4561 agtacacgat cttgtcggcc gcccgccata tccttgcgaa gtcctgcatg aaggacggct
     4621 ggtcggtgag ggcgtgcgcg gtctcccagt agctcatcac ctcatacatg cggcgcccgt
     4681 agaggtaggt gccgatcggc cgctcgaggt cgttgacgaa agtgtgcacc tcctcgtccg
     4741 gcgcggccca gtcgaagttg ccgtctttgt cggcaacgta gccgtcgagc gacgtgatca
     4801 tcgagtagat cagcttgccc atgataagcc tccctttacg ctcaccgctt gcgattcgca
     4861 ccagcccaag aagcataata gggtaatcct cagacctaaa tacagagaac gccgatactc
     4921 tgaacttcgg acgttgcctc ttctgcgaaa ttatctacca accgcgtccc gtcttgcgtg
     4981 gcaagcactg gatctcggcc tactcttggc cggacgctgc gtcgcttggt gggcgctgcg
     5041 aagaggttat gctagcgcca gcattctccg catctcgggg tcaaagaggt tctcaggaga
     5101 ggctccaccc cgagacgatc caatagcagg aactcctctc caataacgcc gttggttccc
     5161 ggattacgtc gtgtaccctt gctgtaccat gtatcctcag acgcacagga gcctcaccaa
     5221 ggccagggag ttcatgcgcc gcgcctacgg gcgtcccgtc tctctacccg atgtggcggc
     5281 ccaggccaac ctctctccct atcattttct gcgcgtctac aagcgggcct acggggagac
     5341 accacacgag tttctaacgc gtctacgcat agagagggcg aagacgctgc tggcgagagg
     5401 aagtcacaac gttaccgaaa cctgcttcga ggttggcttc tccagcctcg gcagcttcag
     5461 cgttttgttc gcgtatcgcg tcgggctatc cccctccgag tacaggcgct acgcccgctc
     5521 gacgatctcc gttccttcta cggtcagggc gctgttcgta ccttcgtgct ttttcaccat
     5581 gttgtacggg actcaggaag agcgcaatat tcgagaagca gggaggctgg agccgatggt
     5641 atcttcgggc cagagcagcg agaccgggag gtaagccgtg atccaacgcc agagccactc
     5701 tacgatctac gtgatcgacc aggaagaagc cctcaggttc taccgcgaca ttctgggctt
     5761 cgaggtaaac actgacatgc ccatggatga ctcggggctt cgcaggctga cgttgaaccc
     5821 gaaaggccag cccgacctcg agctgatcct ggcccaggtg acctcaggac cgatgttcga
     5881 cgccgggacg gccgaagaga tgagagcgct ggtcgagaga ggcgcgttcg gcatcggcgt
     5941 cttcgagaca gacgacatcc aaagcgacta cgagcgcttg agcagccagg gtgtcgagtt
     6001 cgtgtcgcca ccccaggaac ggttctacgg cacagaggcc atcgtcaaag acaactcagg
     6061 gaactggttt agtctcaccc agagcaaacg atagaccatc caaataacgc cagagcaagg
     6121 aggtgtatgc cgccggggtc tcggcgtgca cggtatgtcc cactccgggg acgactgcgg
     6181 attttattcg tgggttgatg ctcgccatgc gcgatgagat cccggcgtac ttctcatcca
     6241 gccctccggc gacggcgagc gcc
//
LOCUS       DATMJA010000275         3394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_865761, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000275 DATMJA010000000
VERSION     DATMJA010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865761"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VNT12_06530"
     CDS             complement(<1..818)
                     /locus_tag="VNT12_06530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWI45809.1"
                     /translation="MIRFADRFVLEHELGSGGMARVFLGRDEVLDRPVAVKLLNPVHG
                     GTDIGDRFEREGRTAARLAHPNIVQVYDAGEAEFNGRETSYIVMEYVPGGDLKGLIDR
                     RGRLPGPELARLGEEICAGLAHAHERGVIHRDIKPHNILLDENGHVKVTDFGIARALD
                     TTQATRTGSFLGTALYSSPEQLQGQKVTPKSDVYSLGATLYQAAAGEPPFTGQSPIEI
                     ASQHVSQNPTPPRSRSADVSVGLEALILNCLAKTPDDRPTAEEARSRFEAEIRPA"
     gene            complement(883..2511)
                     /locus_tag="VNT12_06535"
     CDS             complement(883..2511)
                     /locus_tag="VNT12_06535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWI45810.1"
                     /translation="MGPVGSMGRLGDRFVLVRELGSGGMSRVFLGRDEVLDRPVAVKI
                     VEPDPDDPEIGLRFQREGRSAARLSHPNIIRVFDAGEDELDGRAVSYIVMEYVPGGDF
                     EDLIDRNGPLPETILLRIGADVASGLAHAHERGIIHRDIKPHNILLDERGSPKLTDFG
                     IARALEGTTSHNRASSYLETAAYSSPEQLRGERVTPKSDIYSFGATLYHAAVGVQPFS
                     GNLMEVANQHILKVPVSPRERGARIGKRLETLILGCLAKRPSERPDASLLHDRLLQNG
                     AAEATAALVGPATRVRDGGLPRAAGTSRQTGEAGASGVFRRGPAAQSPSSGPPESTIS
                     LPTRTFRAGYQQRTMLAFLVAALLLLLLTVAGAWALLGQEEQGGGQGPGRNEQAAGGG
                     PQKSKEEPGKGAGEAVQNPSGGSGASDSAQNENQAPAPPLVRAEKVVYNLYYQESFNR
                     VDASWAYLSQRLQNEIGSPERWAEREDIYTLTYMEFTSLPVASAVGDTAEVTFEVRLD
                     HTWGSESLSGTWVCVNEGGEWKLDSLEHAQRVRV"
     gene            complement(2520..>3394)
                     /gene="aroF"
                     /locus_tag="VNT12_06540"
     CDS             complement(2520..>3394)
                     /gene="aroF"
                     /locus_tag="VNT12_06540"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWI45811.1"
                     /translation="SSSCIASATLRSSAGSFPGNMDVSAGVKTKVKTTNGMIRPDTFR
                     VIAGPCTVESYEQFVASAKAVRQAGFAYVRGGAFKPRTSPYSFQGLGEEGLKILSEVS
                     GDLGLKVVTEVMDPYDIEMVMEHAEILQIGARNMANFSLLRHVGGAIAGTDHGVVLKR
                     GLSATVEEWLLAAEYITAAGGDNVVLCERGIRTFETSTRFTLDLSAVIVAKRLTDLPV
                     IVDPSHAAGRRDLVVPLSKAAVAAEADGLMVESHHEPQEALCDGEQALPVEALMGMKD
                     VLQPFASAMGREVI"
BASE COUNT          579 a         1107 c         1055 g          653 t
ORIGIN      
        1 gcgggacgga tctccgcttc gaacctggaa cgggcttctt cggcggtcgg acgatcgtcg
       61 ggggtctttg cgaggcagtt gaggatcaaa gcctccaggc ctacgctcac gtcagcgctt
      121 cgtgagcgtg gcggggtagg attttgcgag acgtgctggc ttgcgatctc tatggggctc
      181 tggccggtga acggcggttc acccgccgct gcctggtaca gcgtcgcccc cagcgaatag
      241 acgtcgctct ttggtgtcac cttctgccct tgcagttgtt cgggcgagga gtacagggcc
      301 gtccccagga aggagccggt acgcgtcgcc tgcgttgtgt ccagagcgcg ggctatgccg
      361 aagtcggtta ccttgacgtg cccgttctcg tcgagcaaga tgttgtgggg cttgatgtcg
      421 cggtggatca caccgcgctc gtgggcgtgg gccagcccgg cgcaaatctc ctcaccgagc
      481 ctggctagct cgggcccggg taaccggccc ctccggtcta taagcccctt gaggtctccg
      541 ccaggaacgt actccatcac gatataggag gtctccctac cgttgaactc ggcttctcct
      601 gcatcgtaga cctgcactat gttcgggtgt gcgaggcggg ccgcggtgcg tccttcacgc
      661 tcaaagcggt cgccgatgtc cgtacctcca tggacagggt tgagcagctt cacggccacg
      721 ggacggtcca gtacctcatc acgtcccagg aagacgcggg ccatgcctcc agagcccagt
      781 tcgtgctcca agacgaacct gtctgcgaac ctgatcacgc gcgtgattat agggtaagga
      841 cggcgcgatg ccgaacggcc gagacatcct gtaccgggtc ggctacacgc gcaccctctg
      901 cgcgtgctcc agactgtcca gcttccactc cccaccctcg ttgacgcaga cccatgtccc
      961 ggagagagat tcagatcccc aggtgtgatc caacctcacc tcgaaggtga cctcggcggt
     1021 gtcaccaacg gcgctggcca caggcaacga ggtaaactcc atgtaggtca acgtgtagat
     1081 gtcttcacgc tcagcccacc gctctggaga acctatctcg ttctgtaacc gctgggagag
     1141 gtacgcccac gaagcgtcta ccctgttgaa agactcctga tagtagaggt tgtatacgac
     1201 tttctcggcc ctcacgagcg gcggggctgg agcctggttc tcattctggg cactgtccga
     1261 ggcccctgag ccacccgagg gattctggac cgcctcaccg gctcccttcc ccggctcttc
     1321 tttggacttc tgcggtccgc cgcccgcggc ctgttcgttc ctgccggggc cctgtccgcc
     1381 gccttgctcc tcctgaccta acagcgccca ggcgcccgca acggtaagta gcaagaggag
     1441 gagggcagcc acgagaaagg ccagcatcgt cctctgctga tagccggccc tgaaggtgcg
     1501 tgtcgggagg cttatcgtac tctcgggtgg gcctgaagaa gggctctgag cggccggccc
     1561 tcgtctgaag acaccagaag ccccggcctc ccctgtctgg cgtgaggtcc ccgcggcacg
     1621 agggaggccg ccgtccctaa ctctggtcgc cggtccgacc agtgcggcgg tcgcctcggc
     1681 cgcgccattc tggagcagcc tgtcgtggag aagggacgca tcggggcgct cggaaggcct
     1741 tttggcgagg caaccaagga tcagagtctc gagccgcttg ccgatccgcg ctcccctctc
     1801 cctgggagat acgggaacct tcagtatgtg ctggtttgcg acctccatga ggttgcccga
     1861 gaagggctgt accccgaccg ccgcgtggta gagggtagca ccgaacgagt agatgtcgct
     1921 cttgggggtg accctctcac ccctcagctg ctctggggag gaataagctg ccgtctcgag
     1981 gtaagagctg gcgcgattgt ggctcgtcgt cccttcgagg gcccttgcta tgccgaagtc
     2041 cgtaagtttg ggactccccc gctcatcgag cagaatgttg tgcggcttta tgtcgcggtg
     2101 gataatgccc cgctcgtggg cgtgggcgag tcccgatgcg acgtcggccc caatccgcaa
     2161 gagtatcgtc tcggggagag gtccgttcct gtcgatcaga tcctcgaagt cgcccccagg
     2221 gacgtactcc atgacgatat aggacactgc gcgcccatcg agctcgtcct cgcccgcgtc
     2281 gaagaccctg atgatgttgg ggtgggagag cctggcggct gacctaccct cgcgctggaa
     2341 tcgcagaccg atctcagggt catcgggatc cggttctacg atcttgacgg cgacgggacg
     2401 gtcgagtacc tcgtcgcgtc ccaggaagac acgggacatc ccgccagagc ccaactcgcg
     2461 cacgagcacg aacctgtccc ccaggcggcc catagagccc acgggaccca tgcccgagac
     2521 tagataacct ccctgcccat ggcactggcg aaaggttgca acacgtcctt catacccatg
     2581 agggcctcga cggggagcgc ctgctccccg tcacaaagcg cctcctgcgg ttcgtggtgg
     2641 ctctccacca tcaggccgtc ggcctcggcc gcgaccgcgg ctttagacag cgggacgacc
     2701 aggtcgcgcc gtccggcggc gtgcgaaggg tccacgatga cgggcagatc cgtcagcctc
     2761 ttggccacga ttaccgccga gagatccagc gtgaaacgcg tgctcgtctc gaaagtccgg
     2821 attccgcgtt cgcacaagac tacgttgtcc ccacccgccg ccgtgatgta ctcggccgcg
     2881 agcagccact cctcgaccgt cgccgagagc cccctcttga gaacgacgcc gtgatccgtg
     2941 ccggctatcg ccccgccgac gtgcctcagc agggagaagt tggccatgtt gcgggcgccg
     3001 atctgcaata tctccgcgtg ctccatgacc atctctatgt cgtaagggtc cataacctcg
     3061 gtgactacct tgaggccaag gtcccctgag acttccgaga gtatcttgag cccctcctcg
     3121 ccgagtccct ggaaggagta aggagaggtg cggggcttga aggcgccgcc gcggacgtag
     3181 gcgaagcccg cctgccttac ggccttcgca gaggcgacga actgctcgta actctcgact
     3241 gtacaggggc ccgcgatcac cctgaaggtg tctggccgga tcatgccgtt ggtggtcttt
     3301 accttggtct ttacgcctgc cgaaacgtcc atgttacctg ggaaactccc cgcgctggat
     3361 ctcaaggtcg cggatgcgat gcaggatgag ctcg
//
LOCUS       DATMJA010000276         4433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_36676, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000276 DATMJA010000000
VERSION     DATMJA010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36676"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VNT12_06545"
     CDS             <1..1319
                     /locus_tag="VNT12_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWI45812.1"
                     /translation="SENTAPTTAIAANLNGFKELTGINVNVLQLELGALVQKVALDIG
                     SGEGAYQIVYADPYQIMAPYHEALADLNEFDSDDDLPSIPKGIDDFIPTQLAAAGRFG
                     NEKNLYALPYDCPTMIWMYRKDLFDKYGDQISQDLGFDPQPSPSLTWEQYYQIANWFD
                     ENQDEVPYGTGHQARQYDSLMCDFSNILWAYGGDYFDSAGIGSLGTTNPGPCTLDEPK
                     ALEAAAFYKKLLSIAHPGSVSWDWNATDEAFRTEQVAMVPNWHEFAAVDQDPAESTIV
                     GKIGYSPLPKGPARNANMWGGTGVGINGASPINEQKAAWLFLVWATSPDTMLMGLKSD
                     VGGGTPTRGSLYELPEVKKAMEPPSNLPNLLTYDAVSEAWKPENIGLRPKIQSWNECD
                     TAIYTEVSKMLAGQKSPEQAMKTAKQYFDEAQARVKNLASGPSGVA"
     gene            1319..2248
                     /locus_tag="VNT12_06550"
     CDS             1319..2248
                     /locus_tag="VNT12_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWI45813.1"
                     /translation="MAATQTEGRGPSTNMKGAGGGGGISLETKMLVPVLLLLAVLSLF
                     PFFYIIWMSLNIVSLINGITFDFAGLANWTRMFTDEVVGAAWARAALYFVATVGLEMI
                     LGIAIALLVHELVWGKNLVLSLILLPMFMAPVIVGLLGRFLTDSSYGLYAFVLRELGI
                     FESDILGTASSAMPAVILMDVWEWTPLIVLIVLAGLASVNQYVIEAAKVDGANYLERL
                     RYIVAPSIAGVVIVALLVRSMDAIRYFDIITVTTNGGPGNTTKIIPIRLYETAFRFFD
                     LGYAAAIGLGMLVFSILVANVFVRILESRGLAK"
     gene            2245..3090
                     /locus_tag="VNT12_06555"
     CDS             2245..3090
                     /locus_tag="VNT12_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWI45814.1"
                     /translation="MTTSSTSGRGSGKQVILYAILFLVLLWTLVPILWMVLASFKPGI
                     AQLSSTPVFIFKPTTQHYVALFTGGNLGSYFLNSVLAAGLSTVIAVSLGCLAGYGLAR
                     SHFRGKRHVAFWIISTRMAPIAAIVLPLFILFRYVNLLNSIPGLVVAYLTFNLPFAIW
                     LMNAFFTDLPPALEEAAMVDGATRFQAFYRVALPLVTPGIVTTAILCLVFSWNDYAFA
                     ATFSGPNSQTLPIAAAQLNTQTGLSWGQLSAIGTIVVLPMMVVGLAVRRYLVKGLTLG
                     AVTGE"
     gene            3118..4140
                     /locus_tag="VNT12_06560"
     CDS             3118..4140
                     /locus_tag="VNT12_06560"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWI45815.1"
                     /translation="MQNKAAVMHATHDVRLEDVPVPEPGPKEVMVEIRAVGVCGSDVH
                     YYEEGRIGSFVVREPLILGHESMGTVVALGERATRHEVGERVTLEPGVPDGTCRECRA
                     GRYNLCPNVRFFATPPIDGAFTNYVSIHEDFAFHLPDSLSDNAGALMEPLSVGIWACK
                     KARVTAGDHVLVTGAGPIGLLAMQVAFAQGATEVTVTDVAGERLEIARKLGATRALNV
                     AEEPLEVAGLEADVLIECSGNERALGDGIRCVRPAGFAVVVGMGPNEETSVPLAFIQT
                     SEITLTGTFRYANTYPTAIDLAATGKVDLDAMVTSHYGLAETEEALQASGKDPANVKP
                     MVIPSS"
BASE COUNT          841 a         1411 c         1348 g          833 t
ORIGIN      
        1 tctcggagaa cacggccccg actactgcca tagccgcgaa cttgaacggc ttcaaggagc
       61 tcaccgggat caacgtcaac gtgttgcagc tggagcttgg ggcgctggtt cagaaggtcg
      121 cgctcgacat cggttcggga gagggcgcct accagatcgt ctacgcggac ccctaccaga
      181 tcatggcccc ctaccacgag gcgctagccg accttaacga gttcgatagc gacgacgatc
      241 taccttccat ccccaagggc atcgacgact ttatcccgac ccagctcgcg gctgcggggc
      301 gcttcgggaa cgagaagaac ctctacgcgc tcccctacga ctgtccgact atgatctgga
      361 tgtaccgtaa agacctcttc gacaagtacg gcgaccagat cagccaggac ctcggcttcg
      421 acccgcagcc ctcgccatcc ttgacgtggg agcagtacta ccagatcgcc aactggttcg
      481 acgagaacca ggatgaggtc ccctacggca cgggccacca ggccaggcag tacgactccc
      541 tcatgtgcga cttctccaat atcttgtggg cctacggcgg cgactatttc gacagcgccg
      601 ggatcgggag cctggggacg acgaatccag gcccgtgcac gctcgatgag ccgaaagcgc
      661 tggaggccgc cgcgttctac aagaagttgc tgtccatcgc gcaccctggc agcgtatctt
      721 gggactggaa cgccacggat gaggctttca ggacggagca ggtcgcgatg gtaccgaact
      781 ggcacgagtt tgccgccgtt gaccaggacc ctgccgagtc gacgatagta ggcaagatcg
      841 ggtactctcc cctccccaaa ggcccagcta gaaacgccaa catgtggggc gggacgggcg
      901 tcggtataaa cggcgcctcg ccgatcaacg agcagaaggc agcgtggctg tttttagtct
      961 gggccacgtc gcctgacacg atgctgatgg gcctgaagag cgacgtcggg ggcggtaccc
     1021 cgacgcgggg gtccctctac gaactaccgg aggtgaagaa ggcgatggaa ccaccgtcga
     1081 acctgccgaa cctgctcacc tacgacgccg tctccgaggc gtggaagccc gagaacatcg
     1141 ggctcaggcc caagatccaa tcgtggaacg aatgcgacac ggccatctac accgaggtct
     1201 ccaagatgct ggccggccag aagtccccgg agcaagcgat gaagacggcc aagcagtact
     1261 tcgacgaggc gcaagcgagg gtcaagaacc tcgcctcagg accgtcgggg gtcgcgtaat
     1321 ggccgccaca cagacagaag gacgcggtcc ttccactaac atgaagggtg ccggcggcgg
     1381 gggtggaatc tcgctggaga cgaagatgct cgttcctgtc ctcttgctgc tcgctgtcct
     1441 ctcgctcttc ccttttttct acatcatctg gatgagcctg aatatcgtct cgctcataaa
     1501 cggcatcacc ttcgacttcg ccgggctcgc caactggacc cgcatgttca ctgacgaggt
     1561 cgtcggcgcg gcctgggcgc gggccgcact gtacttcgtg gccaccgtag ggttggagat
     1621 gatcctgggc atagccatag ccctgctcgt ccacgagctc gtctggggca agaacctggt
     1681 gctctcgctc atcctcctgc ccatgttcat ggcgccggtc atcgttgggc tcctcggacg
     1741 cttcctcact gactcgtcct atggcctcta cgccttcgtc ctgcgcgagc tcggcatctt
     1801 cgagagtgac attcttggga cggccagctc ggcgatgccg gccgttatcc tgatggacgt
     1861 ctgggagtgg acccccttga tagtcctgat cgtgctggca gggctcgcct cggtgaacca
     1921 gtacgttata gaagcggcca aagtggacgg ggcaaactac ctggagaggc tgcgctacat
     1981 cgtggccccc tccatagcgg gcgtcgtgat cgtggctttg ctcgtgcgtt cgatggacgc
     2041 catccgctac ttcgacatca tcaccgtcac taccaacgga ggaccaggta acacgaccaa
     2101 gatcatcccg ataaggctct acgagacggc cttccgcttc ttcgacctcg gatacgctgc
     2161 ggccatcggg ctggggatgc tggtgttctc gattttggtg gcgaacgtgt tcgtgcggat
     2221 cctcgagagc agggggcttg cgaaatgacg acttcttcta cttcgggccg cggttctggc
     2281 aagcaggtaa tactctacgc catcctcttc ctcgtgctgc tctggacgct ggtgccgata
     2341 ctgtggatgg ttcttgcgtc gttcaagcca ggtatcgccc aactctcatc gaccccggtc
     2401 tttatcttca agccgaccac gcaacactac gtggcgctgt tcaccggcgg caacctcggg
     2461 tcctacttcc tgaacagcgt gcttgcggcc ggactctcga cggtcatagc cgtgtccctc
     2521 ggttgcctcg ccggttacgg gctcgcgagg agccacttcc gcggcaagcg gcacgtggcg
     2581 ttctggatca tctcgacgcg catggccccg atagcggcga tcgtactgcc gctcttcatc
     2641 ctcttccgct acgtaaacct gctcaactcg atacctggcc tcgtcgtcgc ctacctgacg
     2701 ttcaacctgc ctttcgcgat ctggctcatg aacgctttct tcacggactt gccgcccgct
     2761 ctggaagagg cggcgatggt cgacggcgcc acgaggttcc aggctttcta ccgcgtcgcc
     2821 ctgccactgg ttacacctgg catcgtgacg acggccatac tctgcctggt cttctcctgg
     2881 aacgactacg cgttcgcggc gaccttctca ggccccaaca gccagaccct ccctatcgcc
     2941 gcagcccagc tcaacaccca gacaggcctg agctggggcc agctctcggc gatcggtact
     3001 atcgtcgtgc tccccatgat ggtcgtcggc ctcgccgtga gacgttacct ggtaaagggc
     3061 ctcacgctcg gcgcggtaac gggagagtaa gagccgcact agaaaggaag cttctctatg
     3121 caaaacaaag cggcggtcat gcacgctacg cacgacgtca ggctggaaga cgtgcccgtc
     3181 cccgaacctg ggccgaagga agtaatggtc gagatccggg ccgtcggggt ctgcggctcc
     3241 gacgtccact actacgaaga aggacgcatc ggctccttcg tcgtaaggga gcctctcatc
     3301 ctcggccacg agtcgatggg tactgtcgtg gccctaggag agagagcgac caggcacgag
     3361 gtaggcgagc gcgtcaccct ggagcccggc gtccccgacg gaacctgccg cgagtgccgg
     3421 gcaggccgct acaacctctg cccgaacgtg cgcttcttcg caacgccccc catcgacggg
     3481 gccttcacca actacgtcag catccacgaa gacttcgcct ttcacctacc tgacagcctt
     3541 tcggacaacg ccggcgcgct gatggagcct ctttcggtcg gtatctgggc ctgcaaaaag
     3601 gcaagggtga cggcgggcga ccacgtcctg gttaccggtg ctgggccgat agggctcctt
     3661 gcgatgcagg tggctttcgc ccagggtgcg acggaggtca cggtcactga cgtcgcgggc
     3721 gagcggctgg agatcgcacg caagctcggc gcgacccgcg ccttgaacgt ggccgaagag
     3781 cccctggaag tggccggatt ggaggccgac gtcctgatcg aatgctcggg caacgagcgg
     3841 gccctcggcg acggcattcg ctgcgtcagg ccagccggct tcgccgtggt ggtagggatg
     3901 ggtccgaacg aggagacgag cgtgccgctc gcgttcatcc agaccagcga gatcactctg
     3961 acgggcacgt ttcggtacgc gaacacctac ccgacagcta tagacctggc cgcgacgggc
     4021 aaggtcgatc tggacgccat ggtgaccagc cactacggcc tcgcggaaac cgaagaggcg
     4081 cttcaggcca gcggcaagga ccccgcgaac gtcaaaccca tggtgatacc ttccagctag
     4141 tcagcctgtc agcacgccgc taggcttcgc cagacggctt gtcagcattt cagcttgtgg
     4201 ttttcgcttt tggggtgttg gacagtccgt actgcacagg tcaaggctgc aggagaaccg
     4261 gtagtgggag ctactctgaa aagagcctgt ctctcaccac gaagtataac ccccgtcgat
     4321 gacgaggtta gtgccggtgg cgaagctcgc ggcgtcagag gcaaggtaga ggacggcggg
     4381 ggctatctca tgcggctctg cgaggcgtcc aagcggcgtc gaagagagcc aca
//
LOCUS       DATMJA010000277         6832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_865787, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000277 DATMJA010000000
VERSION     DATMJA010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865787"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..852)
                     /locus_tag="VNT12_06565"
     CDS             complement(76..852)
                     /locus_tag="VNT12_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45816.1"
                     /translation="MLLEGKTAVVYGGGGKVGGAVARAFASEGARVFLAGRTIGTLEE
                     VAEDIASAGGVAKTAQVDALDEQAIEKHIGEVVERAGSVDISFNAISIQDVQLIPLVE
                     MTREDFMRPISTGTSTHFVTARAAARRMGTQGSGVILTFSASSVRAFFPGVYVGGFGI
                     ACTAIEALTKQLAAEIGPDGIRVNCLRSEGIPESWEGASTGEWSAPPEEIGAFLKQRS
                     LLGRVTTLADVGNAATFLASDRAAATTGTVFNLTSGTVVD"
     gene            complement(894..2387)
                     /locus_tag="VNT12_06570"
     CDS             complement(894..2387)
                     /locus_tag="VNT12_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWI45817.1"
                     /translation="MTQYEVGRHEESHTETMGVGRRWIALYVLCVGMLMIVLDATVVN
                     VALPAIQDDLGFSQSGLAWVVNAYLIAFGGLLLLAGRLGDLISRRGMFLAGIGVFTAA
                     SLLCGLARSQELLVAARFVQGAGGAMTSAVILGMIVTMFPEPREQAKAIGVFAFVASA
                     GGAVGLLVGGVLTQSISWHWIFFVNVPIGIATAVLAGRLIEGDKGIGFGDGADVAGAV
                     LVTGSLMLGVYTIVNPAAEYGWGSGRTLTIGALSLVLLAAFVAREATARNPLVPLRIF
                     RSRNVTGANLIQVLSVAGMFGMFFLGALYLQRILAYDALQTGLAFLPVTILMGTLSVR
                     YTDRLVMRFGARKLVLGGLALFMAGLALFARAPVDGDYVVHILPVMLLLGIGAGLCFP
                     ALMTLAMSGATPSEAGLASGLINTTAQVGGALGLAVLATLSASRSDQLMGDGESVAAA
                     LTSGYHLAFGIGAGLVVVAIAVALTVLRPERQTEEEFQAEPADCEAA"
     gene            2525..3190
                     /locus_tag="VNT12_06575"
     CDS             2525..3190
                     /locus_tag="VNT12_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWI45818.1"
                     /translation="MVTRRTYDDGCAAAHALDLVGERWALLVVRELLLGPKRFTDLRA
                     GLPHASPNVLAQRLRGLEEAGIVRRRRLPPPAASKIYELTDWGEELEAIIIRLGRWGA
                     RSPSKPDYAPLGVDSLILSFRTMFEPGAAEGFEASYELRMGEDRFRAEVSGNSFEVER
                     GSVERPDATIEAESATLAELVYQDRPLTEALRSGDVKIEGDEAAVERLLTLSPLPEPA
                     ALV"
     gene            complement(3253..3492)
                     /locus_tag="VNT12_06580"
     CDS             complement(3253..3492)
                     /locus_tag="VNT12_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45819.1"
                     /translation="MVFRPAAFEFPPSRGRRSFELKPDGALLEGRIGPTDRPLETQGT
                     WELQGDSLVLHRGPLEAPRIMRIASVEDDRLTIEK"
     gene            complement(3585..4502)
                     /locus_tag="VNT12_06585"
     CDS             complement(3585..4502)
                     /locus_tag="VNT12_06585"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HWI45820.1"
                     /translation="MEEQAVPSRRKCGAAAAYFLAVEAQPAYRQRQRELEARTAQRMA
                     LGPEALAPEAPITIPVVVHVVYNDDSENIPDGQIQSQITVLNQDYGAENADKANVPEV
                     WEALVTDTNIRFVLASEDPAGNSTPGITRTQTNRTGFGINDTVKSADTGGADPWPTDR
                     YLNMWACTLDGGLLGYAQFPGGPPETDGVVILNTAFGTTGTAAAPFDLGRTATHEVGH
                     FFNLYHIWGLSDDCTGGDRVSDTPNAEGPNYGEPNFPQISCNNSPYGDMYMNYMDYVD
                     DRAMCMFTAQLVARMMATLDGPRSGLGQS"
     gene            complement(4714..5889)
                     /locus_tag="VNT12_06590"
     CDS             complement(4714..5889)
                     /locus_tag="VNT12_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWI45821.1"
                     /translation="MGRERGPVWVLLGLAPLLGLGVLLAAIVLNGAGIDRKDRPPVED
                     LTIDRVSLPTPDEIVVSVTNGGPEPVRVSQVTVNEALWDFRAEPDAKIPPLGSAEITL
                     PYSWVEGEAYGITLITATGTTFSAEVPAAVITPTFSAETFWRYALIGTYVGVIPVALG
                     LLWYPFLRRLGSAGIGAVLALTLGLLVWLVVDTLLEAVEVAGRLPGVFSGVPLVLVAA
                     LLALLGLLGTERLFRRRGETSRLSTSYRIALGIGLHNLGEGLAIGAAFALGEAALGTF
                     LVVGFTLHNITEGVGIAAPILEERPHLAHFAGLALLGGGPAVLGTWIGGFAYSNLASA
                     VLLAVGAGAILQVIYEVTRLLLKDSERSKSPALSAPTLGGFTAGVAIMYATALLVSV"
     gene            complement(5893..>6832)
                     /locus_tag="VNT12_06595"
     CDS             complement(5893..>6832)
                     /locus_tag="VNT12_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWI45822.1"
                     /translation="LGHDGEEHGANHGASHGAADSGGVAAHGGNGTVGDVDLSRFDPS
                     KFMRHFYWGEERTEGGRTVREYELSAEAVEVEVAPGVMYPAWAYNGQVPGPTLRAREG
                     DLLRVVFKNRDAHPHTIHFHGFHPAGMDGVFELVAPGQEFVYEFDAEPFGCHLYHCHV
                     MPLKKHIEKGLYGAFVIDPREGREEADELVMVLNGFDTNFDGANEVYAVNTVAFHHQR
                     HPVELKLGQLVRIYVVNVLEFDFVNSFHTHANFFDYYPTGTRLKPSEFTDTKIFGQGE
                     RGIMEFSYDFPGLFMFHAHVSEFAELGWMGLFDVKE"
BASE COUNT         1293 a         2267 c         2045 g         1227 t
ORIGIN      
        1 caaccagctc gtcgatgact tcctgaacct gcttctggat ttcggtcatg atcccacccc
       61 tttcgaaggc tacgtctagt ctacaaccgt gccgctggtc agattgaaga ccgttcctgt
      121 tgtcgcggcg gcccggtccg aggccaggaa cgtcgcagcg ttgcccacgt cggccagggt
      181 cgtcacccgc ccaagcaaag atctctgctt gagaaacgcc ccgatctcct cgggtggagc
      241 cgaccactct ccggtcgaag cgccctccca ggactccggg atcccctccg aacgcaggca
      301 gtttacgcgg atgccgtccg gcccgatctc agccgcaagc tgctttgtga gagcttcgat
      361 cgccgtgcac gcgatcccga agccccccac atagacacca ggaaaaaacg cgcggacgga
      421 cgaggcggag aacgtcagga tgacgcccga gccctgtgtg cccatgcgcc gcgccgctgc
      481 cctggcggtc acgaaatgcg tcgaggtacc ggtactgatc ggacgcatga aatcctctcg
      541 ggtcatctcg acgagcggga tcaactgtac gtcctggatc gagatggcat tgaaggagat
      601 atctacgctc cccgctcgct ccaccacctc gccgatgtgc ttctcgatgg cctgctcgtc
      661 cagggcgtcg acctgcgccg tcttggccac tcctccagca gaggctatgt cctccgcaac
      721 ctcctcgagc gttccgatag tgcgccctgc gaggaagacc ctggctccct cgctggcgaa
      781 cgcacgggcc accgcgcccc ctacttttcc tccacctccg tagaccactg cggtcttgcc
      841 ctcgagcagc atgttcatcc tcctgtttgt tgaattcccg gcgcgctgga ggcctacgcc
      901 gcttcgcaat cggcgggctc cgcctggaac tcttcctccg tctggcgttc gggccgtagc
      961 acagtgaggg cgacagcaat cgccacgacg actagtccgg ctccgatccc gaacgcgagg
     1021 tggtagccgc tcgtcagcgc cgccgcgaca gactcgccgt cccccatcag ctggtcgctc
     1081 cgcgacgccg acagggtcgc gaggacggcc aggccaagag caccgccgac ctgcgctgtg
     1141 gtgttaatca agccggaggc caaccccgct tcgctgggcg tggcgccaga catcgccagc
     1201 gtcatgagcg cagggaagca gaggccggcc ccgatgccga gaaggagcat cacaggcagg
     1261 atgtggacca cgtaatctcc gtccaccggc gcccgcgcga acagcgctag cccggccatg
     1321 aatagcgcga ggccgcccag caccaacttc ctggccccaa agcgcatgac caggcggtcg
     1381 gtgtagcgga ccgacagggt tcccatcaag atagttacag gcagaaacgc gagaccggtc
     1441 tgcagggcat cgtaggcgag gattcgctgc aggtagagcg cgccgaggaa gaacataccg
     1501 aacatcccag cgacgctcag cacctggatc aggttcgccc ctgtgacgtt gcgggagcgg
     1561 aagatccgta gcggcaccag cgggttgcgc gctgtcgctt cgcgggcgac aaaggccgcc
     1621 aacagcacga gcgagagggc gcctattgtc agggtacgac cggagcccca gccgtactcc
     1681 gccgccggat tgacgatcgt gtagacgccc agcatcagcg agccggttac cagtacagcg
     1741 ccggcgacgt ccgcgccgtc accgaacccg atgcccttgt cgccctcgat cagccgtccg
     1801 gcgagcaccg ccgtcgcgat accgatcggg acgttcacga agaagatcca atgccaactg
     1861 atggactggg tcaggacgcc gccgacgagc aggcctaccg cgccaccggc ggaggcgacg
     1921 aaagcgaaga cgccgatggc tttcgcctgc tcccgcggct cggggaacat ggtgacgatc
     1981 atgcccagga tcaccgctga tgtcatggcg ccgccagctc cctgaacgaa gcgggccgcg
     2041 accaataact cctggctccg cgcaaggcca catagcaagg aggccgcggt gaaaacccca
     2101 atgccggcca ggaacatgcc cctgcgggag atgaggtctc ccagccgccc agcgagcagc
     2161 aacaggccgc cgaaggcgat caagtaggcg ttgacgaccc acgcaaggcc cgactgcgag
     2221 aaaccgaggt cgtcttgaat cgccggcaac gccacgttga ccaccgtggc gtcaagcacg
     2281 atcatgagca tccccacaca aaggacgtat agagcgatcc aacgccgccc aacacccatc
     2341 gtctcagtat gcgactcttc gtgtcttcca acctcatact gcgtcatccg gatcgtctcc
     2401 gtttcccgag atcgcaacat catctggttt gagcatacgg cggtagtcct aaaaaacaac
     2461 cattgatctt cgaaacctga tcagatatgt agacatcagt acttacggtg gtatactcat
     2521 tcgtatggtg acaagacgca catacgatga cgggtgtgct gcggcgcatg cgctcgacct
     2581 tgtaggtgag cgctgggcgc tcctggtagt acgcgagttg ttgttggggc cgaagcggtt
     2641 cacggacttg cgggctggac tgccgcacgc cagtcccaac gtcctcgccc agcgcctgcg
     2701 cgggttggag gaggctggga tcgtgcgccg tcgaaggctt cctccgccgg cggcgtctaa
     2761 gatctacgag ttgaccgatt ggggtgagga attggaggcg atcattatcc gtctcggcag
     2821 gtggggtgcc cgttccccgt ccaaacctga ctacgctcca ctaggtgttg actcccttat
     2881 cctctccttc aggacgatgt tcgaacctgg cgcagccgag ggcttcgagg cgagctacga
     2941 acttcggatg ggggaagacc gcttcagggc cgaagtgtca gggaacagct tcgaggtcga
     3001 acgcggcagt gtggagcggc ctgacgcgac catcgaggcc gagtcggcta cgctggccga
     3061 gctcgtctac caagatcggc cactgacaga agcgctgcga tcgggggacg tgaagatcga
     3121 aggcgacgag gcggcggtag aacgccttct aacgctctcc cccctgcctg agcctgccgc
     3181 actcgtataa aggtagcgtc ccaacccgcc agggcggtgt tctttacaca taggcggcgt
     3241 ttacaacgcg acttactttt ctatagtcaa ccggtcgtct tccaccgaag ctatccgcat
     3301 aatgcggggc gcctctaaag gaccccggtg gagaacgagg ctgtcaccct ggagctccca
     3361 ggtaccctgc gtctctagag ggcgatcggt cgggccgatc ctgccctcca gaagggcgcc
     3421 gtcgggcttc agctcgaacg accttcggcc acgggagggc gggaactcga aggccgccgg
     3481 ccggaacacc atctccctgt tcgtgtcttc ttcgtgcgaa tgtacccagc gccgccgcaa
     3541 aatcttttcg ctgtctccct ctctcatcgc gacgcggcgt aggtctacga ttggccgaga
     3601 ccgcttcgtg gaccatccag cgtggccatc atccgcgcca ccagttgggc agtgaacatg
     3661 cacatggctc tgtcgtccac gtagtccata tagttcatgt acatgtcgcc gtatgggctg
     3721 ttgttgcagg agatttgcgg aaagttcggt tctccgtagt ttggtccctc agcgttcggc
     3781 gtatccgaga ccctatctcc tccggtgcag tcgtcactga ggccccagat gtgatacagg
     3841 ttgaagaagt gcccgacttc gtgagtcgcc gttcgtccca ggtcgaacgg ggcggccgcc
     3901 gtacccgtcg tgccgaatgc cgtgttgagg atgacgaccc catcggtctc cggtggacca
     3961 ccgggaaatt gggcgtagcc gagcaatccg ccgtcgagtg tacacgccca catgttcagg
     4021 tacctgtcgg tcggccacgg atcggccccg ccggtgtccg ccgacttcac cgtgtcgttg
     4081 atgccgaaac ccgttcggtt ggtctgcgtc cgggtgatac ccggggtgct gttaccagca
     4141 gggtcctccg acgcgaggac gaatcggatg ttggtatcgg tgaccaatgc ttcccacact
     4201 tcgggcacgt tggctttgtc cgcgttttcg gccccgtagt cctgattcag tactgtgatc
     4261 tggctctgga tctggccgtc agggatgttt tcggagtcat cgttgtagac aacgtggacg
     4321 acgaccggga tggtgatcgg cgcttctggt gcgagggctt ccgggccgag ggccatcctc
     4381 tgcgcagtcc tagcctccaa ctctctctgt ctctggcggt acgcgggttg cgcctcgaca
     4441 gccaggaagt aggcggctgc cgcgccgcat ttacgccttg aaggtacagc ctgttcttcc
     4501 atcacagctc cttggggttg gtgtgtacag ggagattgac ctatggccct tgctggtggc
     4561 ctttacctcc ttcgtctagt cagacaatta cctcttgaac cggcaggcga tgaattccga
     4621 atgaatcaac catggttgat cgaacaaggc gggaaaacat cgaagcgtag actcctcgcg
     4681 gagaaggacc ctcgtcgagc cccgactttc agcctaaacg ctcaccagca aagccgtggc
     4741 gtacatgatg gccaccccgg cggtgaaccc gcccagggtc ggggcggaga gggcggggga
     4801 cttcgaccgt tccgaatcct tcaggagaag ccgcgtgacc tcgtagatca cctgtagtat
     4861 ggcgccggcc ccgacagcga gcaacacggc cgaggcgaga ttggagtagg cgaagccacc
     4921 gatccaggtc cccaggacag caggcccacc gccgagaagc gcaaggcctg cgaagtgggc
     4981 gagatgtggc ctctcctcga ggatgggcgc agcgatgcct acgccctctg taatgttgtg
     5041 taacgtgaac ccgaccacga ggaacgtccc cagcgccgcc tccccgaggg cgaacgccgc
     5101 gccgatggcc agcccctccc ccaggttgtg cagccctatc cccagtgcaa tccggtatga
     5161 ggtcgagagt cggctcgtct ccccgcgtct tctgaacaac cgctcggtcc ccaacagccc
     5221 gaggagggcg agcagtgccg ccactagcac cagcgggacg ccggagaaga ccccgggcaa
     5281 ccgcccggct acctccactg cctcgagcag ggtgtcaacg accagccaga ccaggagtcc
     5341 cagcgtgagg gccagtacag cccctatccc cgcggagccg aggcgtctca gaaacgggta
     5401 ccagagcaac ccgagggcca ccggtatcac gccgacgtag gtcccgatca gcgcgtagcg
     5461 ccagaacgtc tccgccgaga acgtcggtgt gatcaccgcc gcaggcacct cagcggaaaa
     5521 ggtggtccct gtggccgtga tgagggttat cccgtatgcc tcgccctcga cccaggagta
     5581 aggcaaggtg atctccgccg aaccaagcgg cgggatcttg gcgtcaggct ccgcccggaa
     5641 gtcccagagc gcctcgttca ccgtcacctg gctcacccta acaggctcgg gaccgccgtt
     5701 ggtcacgctc accactatct catctggcgt gggcaacgac acccgatcta tggtgagatc
     5761 ctctacgggc ggacggtcct tacggtctat ccccgccccg ttcagcacta tggcggccag
     5821 cagtacgccc aaccccagca gcggcgctag ccccaggagc acccagaccg gtccacgctc
     5881 tcgtcccatc tcctactcct tcacgtcgaa gaggcccatc caaccgagct cggcgaactc
     5941 gctgacgtgg gcgtggaaca taaagagtcc ggggaagtcg tagctgaact ccatgatgcc
     6001 gcgttcccct tggccgaata tcttggtgtc tgtgaactcg gaaggcttga gcctggtccc
     6061 cgtcggatag tagtcgaaga agttggcgtg cgtgtggaag ctgttgacga agtcgaactc
     6121 gagcacgttg accacgtaga tcctcaccag ctgcccaagc tttaactcga cggggtggcg
     6181 ctggtggtgg aaggccactg tgttcacggc gtagacctcg ttcgcgccgt cgaagttggt
     6241 gtcgaagccg ttcaagacca tcacaagctc gtcggcctcc tcgcgcccct ccctcgggtc
     6301 tatgacgaaa gcaccgtaga gccccttctc gatgtgcttc ttgaggggca tgacgtggca
     6361 atggtagagg tggcacccga acggctcggc gtcgaactcg tagacgaact cctgccccgg
     6421 cgcgacaagc tcgaacaccc cgtccattcc cgcagggtgg aagccgtgaa agtggatcgt
     6481 atgcgggtgc gcatccctgt tcttgaacac cacccgcaac aggtcgccct ccctggcgcg
     6541 caacgtgggc ccaggcacct gcccgttgta ggcccacgcc gggtacatca ccccaggcgc
     6601 cacctctacc tccaccgcct ccgcactcaa ctcgtactct cgaaccgtcc tgccaccctc
     6661 ggtccgctcc tcaccccagt agaagtgcct catgaatttc gagggatcga acctcgagag
     6721 gtccacatcc ccaaccgtcc cgttgccccc gtgcgcggcc acgccgccgg agtctgcagc
     6781 cccgtggctc gccccatggt tcgccccatg ctcttctccg tcgtgcccga gg
//
LOCUS       DATMJA010000278         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_451143, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000278 DATMJA010000000
VERSION     DATMJA010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451143"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VNT12_06600"
     CDS             <1..178
                     /locus_tag="VNT12_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45823.1"
                     /translation="RMSGGLIEKFEERMLQGIPLHRFGNPEDLKGVVIFLASPAAAYI
                     TGQTVVVDGGATAW"
     gene            234..1520
                     /locus_tag="VNT12_06605"
     CDS             234..1520
                     /locus_tag="VNT12_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI45824.1"
                     /translation="MKRTTVPVMKSVELPNGVAVPYAEQGDTSGYPVVLLHAIADSWR
                     SFERVLSDLPESIHAFALTQRGHGDASRPARGYHSRDFAADLAAFMDALHLEAAVVVG
                     GSSGGFVARRFAIDHPERTLGLVLLGSPAILRDKPGVLEMWDSTISELKDPISPDLVR
                     EFAEGTLTRPVPQAFLETIVQENLKAPARVWRATFEGLLEDDSLEELDKIKAPTLIVW
                     GDRDAILPRSDQETLATAITGSRLLVYPGAGHAFYWEDPGRVASDLAAFIAELETKYH
                     PSEGRINRMSVERNKATFRRYIEEVWKDEQLDIADEIFAEKYLSHQSDGTALERGPED
                     VKKFVMEYRTAFSDIEDIVEDMIGEGDRVVTRWTLRVTHTGEFRGIPATGKRITITGI
                     GIFRFSEEGKVVESWDSLDQLGMLRQLGVPAHQHFS"
     gene            1762..>2433
                     /locus_tag="VNT12_06610"
     CDS             1762..>2433
                     /locus_tag="VNT12_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI45825.1"
                     /translation="MATIDGWLEAFLHGPTALWVVLLVSLALGLRHASDPDHLAAVTT
                     FIASEKDGRGTRKAGSMGLSWGMGHGTSLVLIGLPLVLLGRYLPGPVQQAAEALIGVI
                     IILLAVRLLIRWRRGHFHAHAHTHDGEPVHRHVHSHESDYSHEHAHHVSLRTPLSSYG
                     IGLVHGIGGSGGLTLLLLSTIPDPAQATGALVIFACGTAVSMALLSTAFGLAIASGPI
                     GRNFER"
BASE COUNT          457 a          750 c          748 g          478 t
ORIGIN      
        1 ccgaatgagc ggcggcctga tcgagaagtt cgaagagaga atgctccagg gcattcccct
       61 ccatcgcttc ggcaaccccg aggacctcaa aggagtagtg atcttcctcg cctcgccagc
      121 cgccgcctac atcacaggcc agaccgtcgt cgtggacggc ggcgccacag cctggtaagt
      181 cgttatgccc actcgtggtc gatacgccgg ctcgctattc tgaggagaaa acgatgaaac
      241 gaaccacggt gccagtcatg aagtccgtcg agcttcccaa cggcgttgcg gtcccctacg
      301 ctgagcaggg ggatacgtcg ggctatcccg tcgtgctcct gcacgccatc gcggactcct
      361 ggcgatcctt cgagcgcgtg ctctctgatc tgcccgagtc catccacgct ttcgcgttga
      421 cacagagggg ccatggtgat gccagccgtc ccgcgagggg ctaccattcc cgcgatttcg
      481 cggcggatct ggcggcgttc atggacgcgc tccatctcga ggctgccgtc gtcgttggtg
      541 gttcaagtgg cggcttcgtc gcccggcgct tcgcgataga ccaccccgag cgcaccctcg
      601 gactcgttct tcttggctcg ccagccatcc tgcgagacaa accgggcgtg ttggagatgt
      661 gggactcgac gatctcggag ctgaaggacc cgatctcccc tgacctcgtg cgcgagtttg
      721 cggagggcac gttgaccagg ccggtgccgc aggcattcct cgagactatc gtacaagaga
      781 acctgaaggc cccggcgcgg gtgtggcgcg ccacgttcga gggactgttg gaagacgatt
      841 ccctggagga actagacaag atcaaggccc ccacccttat cgtatgggga gatagggatg
      901 caatccttcc gcgcagcgat caagagactc tggcgacggc gatcacaggc tcccggctcc
      961 tggtgtatcc cggcgccggt cacgccttct actgggagga tccaggccgt gttgcgtcag
     1021 atctcgcggc cttcatcgcg gaactcgaga caaaatatca cccatcggaa ggaaggatca
     1081 acagaatgtc agtcgagagg aacaaggcta cctttaggcg ctacatcgag gaggtctgga
     1141 aggacgagca gcttgacatc gcggacgaga tcttcgccga gaagtatctc tcccaccagt
     1201 ccgacggcac cgctctggag cgcggcccgg aagacgtcaa gaagttcgtt atggagtacc
     1261 gcaccgcttt ctcggatatc gaagacatcg tcgaggacat gatcggagag ggggacaggg
     1321 tggtgacacg ctggacgctg cgcgtcaccc acacaggcga gttcaggggc atccctgcca
     1381 caggcaagcg gattacgatc accgggatcg gcatcttccg tttctcggag gagggtaaag
     1441 tggtagagag ctgggacagc ctcgaccagc tcggcatgct gcgccaactc ggtgttccag
     1501 cacatcagca tttcagctag tcagcctttt gttttcgctc atgcggccat gagcgattcg
     1561 cagtagatca atatccctgc aaatcgctct aatcctccag ccgacaagtt gaaatgctga
     1621 cttgctgagg tggtggcttg ctgctaagct gacatgcctg attttgtgcc gtttgtgatc
     1681 caggtttacg gcgcaagcgt atacgagtcg ccagaagaca gaggcgggcc aagggagccg
     1741 gtcttggaga agggagaggg tatggcgacg atagacggtt ggctcgaggc cttcttgcac
     1801 gggcctacgg ctctctgggt agtactgctg gtaagcctgg cgctgggttt gcgtcacgcc
     1861 tcagaccccg accacctggc cgcggttacc accttcatcg cctccgaaaa ggatggtaga
     1921 gggacgagaa aggccggctc gatgggtctc tcctggggga tggggcacgg cacatccctg
     1981 gttctcatcg gccttccgct cgtgttgttg ggacgttacc tgccggggcc tgtgcagcag
     2041 gctgcggagg ctttgatcgg agtcatcata atccttctcg ccgtacggtt gctcattcgc
     2101 tggcggcgcg ggcacttcca cgctcacgcc cacacccacg acggcgaacc tgtgcaccgc
     2161 cacgtccact cccacgaatc cgactactcg cacgagcacg cacatcacgt ctccctccgc
     2221 acaccgctct cttcctatgg tatcgggctg gtgcatggca tcggcgggtc gggtgggctc
     2281 acgctgctgc tactctcgac catccccgac ccggcgcagg ccacaggggc gctcgtgatc
     2341 ttcgcctgcg ggacggcggt ctcgatggcg ctcctttcga ccgcattcgg gctcgccata
     2401 gccagcggtc ccatcggcag gaacttcgag cgt
//
LOCUS       DATMJA010000279         2352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_865878, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000279 DATMJA010000000
VERSION     DATMJA010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865878"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VNT12_06615"
     CDS             <1..743
                     /locus_tag="VNT12_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWI45826.1"
                     /translation="ALCMETAWIFGAEPSYVLPSAAAIELIHTYSLIHDDLPAMDDDD
                     YRRGRPTAHREFGEAMAILAGDAFFGEALTLITIHQKGSCEQILEVVRELAGSTGVNG
                     MVGGQVMDMDQTGRAEGTDPETLYMIHEYKTGALIKSSARIGAILAGATGEEQAAVSE
                     YASQLGLCFQIVDDVLNATSTREELGKSAGSDAEQGKATFAGVFGLEGAKREADGAAK
                     RALAALSKVDGDISGLQELAYFVRHRES"
     gene            753..1556
                     /locus_tag="VNT12_06620"
     CDS             753..1556
                     /locus_tag="VNT12_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWI45827.1"
                     /translation="MESSPKGSPRRLDALLVERGLAASRSSAQGLILSGSVRVGDVVV
                     TKAGSRIGADEPLSVDQAERFVSRAGEKLDAALGEFGVRVEGRSCLDAGASTGGFTDA
                     LLQRGAERVLAVDVGYGQFDWSLRNDPRVTVMERTNVRTLAAEDLPFDTDLLVADLSF
                     ISLTVALESLLSTTPALREAILLVKPQFEAGPESVGRGGLVRDREVHVSVIQSVTRYF
                     STLGFGAVGATQGAIAGRKSGNLEYPVHLLRGAEPALDEARIREVVGGG"
     gene            1549..>2352
                     /locus_tag="VNT12_06625"
     CDS             1549..>2352
                     /locus_tag="VNT12_06625"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWI45828.1"
                     /translation="MGETMKAARIRRVGIVANSRVSNGYVDRLVAVLEKNRVEISEVR
                     GDDQPDGARDVDLVFVLGGDGTMLRASRMYPGQVLLGVNLGKVGFMSGMLPEELETGV
                     EKVLGDGLEVQEYRMLDVRVGDEELGLAANDAVLIKKRPHQLISVDVSIGGEELFAFR
                     CDGFIAATPLGSTAYALSAGGPIISGDAGCYVLVPIAPHALVSRPLVLGEDHVAELAL
                     VEREALLSLDGAEPREIPAGGKMEIRLSSESVRIGRTEDWSWWRAVRRTL"
BASE COUNT          437 a          693 c          818 g          404 t
ORIGIN      
        1 cggctctttg catggagacg gcctggatct tcggggccga accctcctac gtgctcccat
       61 ccgcggccgc catcgaactc atccacacct actcgcttat ccacgacgac cttccagcta
      121 tggacgatga cgactacagg aggggaaggc cgactgccca cagggagttc ggcgaggcga
      181 tggccatact cgcaggcgat gccttcttcg gtgaggccct gaccctgatc acgatccacc
      241 agaaggggag ctgcgagcag attctcgagg tcgtgcgaga gctggcaggc tcgacggggg
      301 tcaacgggat ggtcgggggg caggttatgg acatggacca gactggccgc gccgagggga
      361 ccgaccccga gaccctgtat atgatccacg agtacaagac gggcgccctg atcaaatcct
      421 cagcccgtat cggggcgata ctggcagggg ccaccggcga ggaacaggcc gccgtctcgg
      481 agtacgcctc gcaactcggc ctctgcttcc agatagtcga cgacgtcctc aacgccactt
      541 cgacgcggga ggagctcggc aagagcgcgg gttccgacgc cgagcagggc aaggccacgt
      601 tcgcgggcgt cttcggtctc gaaggcgcaa aaagggaggc tgacggtgcc gcaaagaggg
      661 ccctcgccgc actctccaaa gtcgacggag acatctcggg tctgcaagag ctggcgtatt
      721 tcgtgcgcca cagagagagt tagcctactc ccatggagag tagcccgaaa gggagtccca
      781 ggcgattgga tgcgctgctc gtcgagcggg gcctcgccgc gagcaggagc agcgctcagg
      841 gtctgatcct ctcaggcagc gtgcgcgtgg gtgatgtggt agtgaccaaa gcaggctcgc
      901 gcatcggcgc tgatgagcct ctgtctgtcg accaggccga acgctttgtg tctcgggccg
      961 gcgagaaact ggacgccgcc ttaggcgagt ttggcgtgcg ggtggaggga cgttcctgcc
     1021 tcgatgccgg agcatcgacg ggagggttta ccgacgcact cctgcaacga ggagccgaga
     1081 gggtgctcgc cgtcgacgta ggctacggcc agttcgactg gtcgctgcgt aacgatccac
     1141 gcgtcaccgt tatggagcgg accaacgtgc gcaccttggc cgcagaagat ctgccgttcg
     1201 acaccgacct actggtggcc gacctctcgt tcatctccct cacggtcgcg ctcgaaagcc
     1261 tactctcgac cactcccgcg ctgcgggagg cgatcttgct ggtcaagccc cagttcgaag
     1321 cgggacccga gtccgtagga cgcggaggcc tggtaaggga cagggaggtg cacgtctctg
     1381 tgatacaaag cgtcacccgg tacttcagta cccttggttt cggcgccgtc ggtgcgacac
     1441 agggggctat cgcaggaagg aagtcgggga atctggagta cccggtgcac ctgctcaggg
     1501 gagcggagcc ggccctggac gaggcgcgca tcagagaggt ggtcggcggt gggtgaaacc
     1561 atgaaggctg cccggatccg cagggtagga atcgtcgcca actccagggt gagcaacggc
     1621 tacgtcgacc ggcttgtggc cgtcctcgag aagaacaggg tcgagatctc cgaggtcagg
     1681 ggcgacgatc agccggatgg agccagggac gtagatctcg tcttcgtgct cggcggcgac
     1741 ggtaccatgc tgcgggcatc gaggatgtac cctgggcagg tgctgctcgg ggtaaacctt
     1801 ggtaaggtcg gtttcatgtc ggggatgctc cccgaagagc tcgagacagg cgtcgagaag
     1861 gtactcggag atgggctcga agtccaggaa tacaggatgc tcgatgtgcg ggtcggagac
     1921 gaggagcttg gcctcgcggc caatgacgcc gtgctcatca agaagcggcc tcaccagctc
     1981 atctccgtcg acgtctccat cggcggggag gagttgttcg cctttcggtg cgacggcttt
     2041 attgcggcga cccccctcgg ttcgaccgcc tacgcgctct cggcgggtgg gccgatcatc
     2101 tcgggtgacg ccgggtgcta cgtgctggtc cccatagcgc cacacgcgct ggtcagcagg
     2161 cccctggtgc tcggagaaga ccacgtcgcc gagctggcgc tcgtcgagcg ggaggcgctg
     2221 ctctcgttgg acggggccga accgcgtgag atccctgccg gtggaaagat ggagatcagg
     2281 ctctccagcg agagcgttag aataggccgt acggaagact ggagttggtg gcgagcagtt
     2341 cggaggactt ta
//
LOCUS       DATMJA010000280         2570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_214587, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000280 DATMJA010000000
VERSION     DATMJA010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214587"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VNT12_06630"
     CDS             complement(<1..601)
                     /locus_tag="VNT12_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45829.1"
                     /translation="MATIEGWLEGFLHGPTTLWVVLLVSLALGLRHATDPDHLAAVTT
                     LIASESGNGARKAGSMGLSWGIGHGTSLVFIGLPLVLLGRYLPGPVQQAAEALIGVII
                     IILAARLLARWRRGHFHAHAHSHDGEPVHRHVHSHESDSWHEHAHNVALRTPLSSYGI
                     GLVHGIGGSGGLTLLLLSTIPNPVQATGALVIFAGGTAVS"
     gene            853..1665
                     /locus_tag="VNT12_06635"
     CDS             853..1665
                     /locus_tag="VNT12_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI45830.1"
                     /translation="MPYATNALDGSRVYFEDDGGDGTPVVLHGGIIDSVDLVRESNIA
                     QALQELPTDEFRLIYVAHRGVGRSDKPHEVEAYAMPLRVADAVAVLDELGIERAHFIG
                     NSWGGRLGFGIGEHAPERVLSLVIGGQQPYAIDPDGPLAHAATETLAESRREGSMEPF
                     VEMLESFAGTRFPDALRERWLDNDPAAIEAAWSAAVAEEAISEDLGAWELRCLIYVAT
                     GDVDFHDQAQQAANEILNAEFISIEESDHVGAHLAEVDPVLAAVLRTLRASV"
     gene            complement(1712..2488)
                     /locus_tag="VNT12_06640"
     CDS             complement(1712..2488)
                     /locus_tag="VNT12_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45831.1"
                     /translation="MNALDLFRLDGSTVIVTGGGRGLGRYMAEALSDAGAGVVLCSRK
                     IEPLEEVRSEIEAGGGRALAVECDVTDPEDVERVVAQAEEAFGSLDIVVNNSGATWGA
                     PPAEMPLERFDQVLRVNVRGTFLMSQAAGRRMIERGSGGTIINISSVAGLVGGHPDYM
                     QTVGYNSSKGAIISMTRDLATSWAPHGITVNAIAPGWFPTRMSGGLIEKFEERMLQGI
                     PLHRFGNPEDLKGVVIFLASPAAAYVTGQTLVVDGGATAW"
BASE COUNT          496 a          787 c          827 g          460 t
ORIGIN      
        1 tcgagaccgc cgtcccaccg gcgaagatca cgagtgcccc ggtcgcctgg accgggttgg
       61 ggatggtaga gagtaacagc agggttagcc cgcccgaccc gccgatgcca tgcaccagcc
      121 cgataccata ggaagagagc ggcgtgcgga gggcgacgtt atgggcatgc tcgtgccagg
      181 agtcggattc gtgggagtgg acgtggcggt gaacaggttc gccgtcgtgg ctgtgggcgt
      241 gggcgtggaa gtgcccgcgc cgccagcgag cgagtaaccg tgcggcgagg atgattatga
      301 tcacaccgat caaagcctcc gcggcttgct gcaccggccc cggcaggtaa cgtcccaaca
      361 acacgagcgg aaggccgatg aaaaccaggg aagtgccgtg cccaatcccc caggaaagac
      421 ccatcgagcc ggcctttctc gccccgttac cactttcgga ggcgataagg gtggtaaccg
      481 cggccaggtg gtcggggtcg gtggcgtgcc gcaagcctag cgccaggctg accagcaata
      541 ctacccagag agtcgtaggc ccgtgtaaga agccctcgag ccaaccctct atcgtcgcca
      601 taccctctcc cttcctccgc acaaccagcc tctttcgtcc ggcgatgcgt atacgcttga
      661 cgcgcaaact cggatcacaa acgttacatt ttcagatagg ttcgaaggcg aatcctcagg
      721 aagtccgcaa cgccgagttt cgaacagcgt ccgagcaaca ctcaaaggta gtcctttcaa
      781 gggcgagcac cgtggaacgg ctcagagcga gtggaataca atggtaatga ggtgatgaga
      841 ggaggagcgc tcatgcctta cgcgacgaac gctctggatg gaagccgggt gtacttcgag
      901 gatgacggcg gcgatggaac cccggtcgtc ctgcatggag ggataattga ctcggtagat
      961 ctggtgcgag agtcgaacat cgcccaggca ctgcaggagc tgcctacgga cgagttccgc
     1021 ctcatctacg tcgcccaccg cggggtcgga cgcagtgaca agcctcacga ggtggaggca
     1081 tatgcgatgc cgttgcgcgt ggcggatgcg gtggcggtgt tggatgaact aggcatcgag
     1141 cgcgcacact tcatcggtaa ttcatggggg ggaaggctcg gcttcgggat cggcgaacat
     1201 gcgcccgagc gcgtgctctc gctcgtaatc ggcggccagc agccttatgc catcgacccc
     1261 gacggcccgc ttgctcacgc cgccacggag actctggccg agtcgcgaag ggaagggagt
     1321 atggagccct tcgttgagat gctggagtcg ttcgccggta cccgtttccc agatgccctg
     1381 cgggaacggt ggctcgacaa cgaccccgca gccatcgagg cggcgtggag cgcagcagtt
     1441 gctgaggagg cgatctcaga agaccttggg gcgtgggaac tgagatgcct catctacgta
     1501 gcgaccggcg atgtcgattt ccacgaccag gcgcagcagg cggcgaatga gatcctcaac
     1561 gcggagttca tctcaattga agaatcagat cacgtagggg cgcacctggc cgaggtcgat
     1621 cctgtgctcg ccgctgttct gcgcacgctg cgtgccagtg tctgaaaacc tgatggcaga
     1681 gccggacgca tccccaaatg aggtatcttt gctaccaggc tgtcgcgccg ccgtccacga
     1741 cgagggtctg gcctgtgacg taggcggcgg ccggcgaggc gaggaagatc actactcctt
     1801 tgaggtcctc ggggttgccg aagcgatgga ggggaatgcc ctggagcatt ctctcttcga
     1861 acttctcgat caggccgccg ctcattcggg tcgggaacca gcctggggct atggcgttga
     1921 cggtgatgcc gtgcggggcc cacgaggtcg ccaggtcacg ggtcatggag atgatggcgc
     1981 ctttggacga gttgtagcct acggtctgca tgtagtcggg gtggccgccg accaggcctg
     2041 ccacgctgga gatgttgatg atggtaccgc cggatccgcg ttcgatcatg cgccgcccgg
     2101 ccgcctggga catgagaaag gtccctcgca cgtttaccct gagtacctga tcgaagcgct
     2161 ccaaaggcat ctccgcgggc ggcgctcccc acgtagcccc gctattattg acgactatat
     2221 cgagactccc gaaagcctcc tcggcctgcg ccacgactct ctccacgtcc tcagggtcgg
     2281 tgacgtcgca ctccacggcc agcgccctgc cgcctccagc ctcgatctcg ctccttactt
     2341 cctcgagagg ctctatcttg cgcgagcaca gcacgacgcc agccccggca tcagagaggg
     2401 cctctgccat gtagcggccc agaccgcgcc cgccaccggt gacgatgacc gtactgccgt
     2461 cgagacgaaa gagatccagg gcgttcaaga cattctcctc ctaactccga ggtgttgcct
     2521 ctgtcttggc gcgttcctcg tctaccagga cgcggcgcaa tagcttgccc
//
LOCUS       DATMJA010000281         3960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_214652, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000281 DATMJA010000000
VERSION     DATMJA010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214652"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VNT12_06645"
     CDS             <1..496
                     /locus_tag="VNT12_06645"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWI45832.1"
                     /translation="DEAGVSKAILPYYFDSKENLTLLTMRWVLARVAGRIREAIDVVE
                     TAEEKVAAMVDAIFVSPESNRRFYLVFFDFLGYAARNDRFADVGATFHEICNGLYAEV
                     IRLGQEEGVFPGCDAREGATVVRALVDGLFTQWIQEKDWEKTHADYREICKRSVLTYL
                     TAGA"
     gene            515..1513
                     /locus_tag="VNT12_06650"
     CDS             515..1513
                     /locus_tag="VNT12_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI45833.1"
                     /translation="MSVAPSYLGTQPIGETSRVSDGVFQVKLPVPFPLKFVASYLVED
                     RDGWTVIDPGFDYPPARETWERGAAEVGLDLERDLARIIVTHLHPDHIGLARWLEERS
                     GAPVWMLEGEIENARRVWDPDRGTEDFVRYLIRNGMDAQTAEPTAETTRLGVRLPERL
                     LPLQQGDSIDLGGSEARVVHTPGHSDFHFILHDEQRRLLFAGDHLLLEITPNIGLWPY
                     TAPRPLRRYLDSIASLRGLDVDIVFPGHGPLFHDLEGRVDELLAHHEERLSVMLAALD
                     ERLATPFEVARRVFPEDLTDHQLRFALAETLAHLEHLEGEGYVEQVADGVVSYQAV"
     gene            1519..1659
                     /locus_tag="VNT12_06655"
     CDS             1519..1659
                     /locus_tag="VNT12_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45834.1"
                     /translation="MLANEEEHTRLVETARGDRPADLFVRGPLGLLDVKSSETLAAAE
                     RL"
     gene            1883..3496
                     /locus_tag="VNT12_06660"
     CDS             1883..3496
                     /locus_tag="VNT12_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HWI45835.1"
                     /translation="MKTVLPGQGFRFELDGRSLTIEDVFAFSRRSESASCALSAEAVE
                     KIKATRALKRDLIGREVPIYGVTTGFGDSAHRQISASKTAGLQQNMLRFLGSGTGPIA
                     SPEVTRAVMLLRANCLARGNSGVRLELVERLLFYLNNDVLPLIPERGSCGASGDLVPL
                     SYVGAALVGEVEVLHDGEVKDSKELLGELGLEPLELEAKEGLALTNGTSFMSAFAALA
                     VWDAEELALVSDLCTAMASEALHGNRGHFNAFIHEQKPHPGQVESARVIRNLLEESEL
                     ALDSEEIFAGDGLGDKEFLELERQVQDKYSIRCAPHVNGALRDTLEWVRRWVEVEINA
                     SDDNPLFDAASGRVRSGGNFYGSHITQAMDSLKIAVANLCDLMDRQLELVVDEKFNAG
                     LTPNLIPFFDEDSPEAGLHHGFKGMQLCCSAMTAEALKLSNPASVFSRSTEAHNQDKV
                     SMGSIAARDARSIIELAQNVAAIHVLAVCQALDLRGVQKASPKTRAAHELVRSRVPFL
                     DGDRRMDTDIKEIVDLIRSGELSKRTLPT"
     gene            3633..>3960
                     /locus_tag="VNT12_06665"
     CDS             3633..>3960
                     /locus_tag="VNT12_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI45836.1"
                     /translation="MLDAKKPWLSIYGDRLSGEPLGGSLTEFLEEAVEKYRANTALTR
                     GEQKISYGELLDLTETFAAALYEAGVRKGDRVGLMLPNCPEYVVAFFGTMRIGATATQ
                     VNPVYVG"
BASE COUNT          731 a         1186 c         1315 g          728 t
ORIGIN      
        1 ggacgaggcc ggggtgagca aggccatcct cccgtactac ttcgattcca aggagaattt
       61 gacgctcctg accatgcgtt gggtgctcgc ccgcgtcgcc gggcgcatcc gcgaggccat
      121 cgacgtggtc gagaccgccg aggagaaggt cgcggccatg gtggacgcca tcttcgtgag
      181 tcccgagtcc aaccgcaggt tctacctcgt cttcttcgac ttccttggct acgcggctcg
      241 taatgaccgc ttcgccgacg tgggcgcaac gtttcacgag atctgcaacg gcctatacgc
      301 cgaggtcatc aggctcggcc aggaggaggg cgtcttcccc ggatgcgacg ctcgggaagg
      361 tgcgactgtg gtgcgggccc tcgtggacgg gctgttcacg cagtggatcc aggagaagga
      421 ctgggagaag acccacgcgg attaccgtga gatatgcaag cgttccgtac tgacctatct
      481 gaccgccggt gcttagaggc gtgcccgtga tctcgtgagc gtcgctcctt cttatcttgg
      541 cacacagccc atcggcgaga cgtcgcgtgt ctcggacggc gtctttcaag tcaaactgcc
      601 tgtgcctttc cccctgaagt tcgtcgcgtc gtaccttgtc gaggaccgcg acgggtggac
      661 agtcatagac cccggcttcg actatccgcc agcccgcgag acgtgggaga gaggcgcagc
      721 ggaggttggc ctggacctgg agcgcgacct ggcacggatc atcgtgacgc atctgcaccc
      781 ggatcacata gggctcgccc gttggctgga agagcgtagc ggggcgccag tctggatgct
      841 cgaaggtgag atcgagaacg cccgccgcgt ctgggacccg gacaggggaa cggaagactt
      901 tgtacggtac ctgatccgga acggcatgga cgcccagacg gctgaaccga cggcagaaac
      961 cacgcggcta ggcgtccgac tgccggagcg cctgctgccg ttgcagcagg gagattcgat
     1021 cgacctcgga ggcagcgagg ccagggtggt gcacacgccg ggccactcag attttcactt
     1081 catcctgcac gacgaacaac ggcggctcct gttcgcagga gaccacctgc tgctcgagat
     1141 cactccgaac atagggctgt ggccatacac ggccccgcgg ccgctccggc gttacctcga
     1201 ctcaattgca agcttgcggg gtctggacgt ggacatcgtc ttcccggggc acggtccgct
     1261 ctttcacgac ctggaagggc gcgtggacga gctcctcgcg caccatgaag agcgcctctc
     1321 ggtaatgctc gcagccctcg acgagaggct ggcgacgcca ttcgaggttg cgcgccgcgt
     1381 tttccccgag gatctcaccg atcaccagct acgcttcgcg ctcgccgaga cgctggccca
     1441 cctcgaacac ctggaggggg aaggatacgt agaacaggtg gcagacggcg tggtcagcta
     1501 ccaggcagtc taacctacgt gctggcgaac gaagaggaac acacgaggct cgtggagacg
     1561 gcgcggggtg atcgtcctgc tgacctcttc gtgcgtggcc ccctgggcct gctcgatgtg
     1621 aaatcgtccg agacactggc ggcggccgag cgtttgtagt ctggatcacc gtaccacgcg
     1681 agccgcattc gcccggcgcc gataccgagg tggcttcgtg gcgttgcggg agacggatct
     1741 cggcgtcgtg aactctcgcc ccggcttcgt cgagtgcctc gccgtgtttc cagggacggt
     1801 tcgggttaaa atactggttg tccgaccggt caatcggtgt cgggacgtgg ggaagcacag
     1861 ccagtacaag gggtgatctg cgatgaagac cgttctaccg ggacagggct tccggttcga
     1921 gctcgacggg agatcgctaa cgatcgagga tgtgtttgct ttctcccgca ggagtgaatc
     1981 tgcgtcgtgc gccctatccg ctgaggcggt ggagaagatc aaggcgacgc gtgcgctgaa
     2041 gcgggatctg atcgggcgcg aggttccgat ctacggcgtc acgacaggct tcggggacag
     2101 cgcgcaccgc cagatctccg cgtccaagac cgccggtctg cagcagaaca tgctccgctt
     2161 tctgggcagc ggaacgggcc cgattgcctc gccggaagtt acgagggcag tgatgctcct
     2221 gcgcgccaac tgcctcgcca ggggcaactc cggtgttcgc ctcgaattgg tggagcgcct
     2281 cctgttctac ctgaacaacg atgtactccc tctcatccct gagcgtggct cctgcggcgc
     2341 cagcggagac ctggtgccgc tttcgtatgt gggcgccgct ctcgttggcg aagtggaggt
     2401 cctgcacgac ggcgaagtca aggactcgaa ggagcttttg ggtgagctgg gcttggagcc
     2461 gctggagctc gaggccaagg aggggctcgc cctgaccaac gggacctcgt tcatgagcgc
     2521 gttcgccgct ctggcggtgt gggacgcgga ggagctggcc ctcgtatccg acctgtgcac
     2581 ggcgatggcg tctgaggcgt tgcacggcaa ccggggacac ttcaacgcct ttatccacga
     2641 gcagaaaccg catcccggtc aggtggagag cgcacgtgtc atccggaacc tgctggagga
     2701 gtccgagctg gcgctggatt ccgaggagat cttcgccggc gacggcctcg gcgacaagga
     2761 gttcctggag ttggagagac aggtgcagga caagtattct attcgttgcg cgccacacgt
     2821 gaacggcgct ctgcgcgaca ctctggaatg ggtgaggcgc tgggtggagg tcgagatcaa
     2881 cgcctccgac gacaaccctc tcttcgacgc tgcgagtggc cgggtgcgga gcggaggcaa
     2941 cttctacggc agccacataa cccaggctat ggactcgcta aagatcgccg tagccaacct
     3001 ctgcgacctg atggaccggc agctcgaact ggtcgttgac gagaagttca acgctggcct
     3061 gaccccgaac ttgatccctt tcttcgatga ggacagcccc gaggcgggtc tgcaccacgg
     3121 cttcaagggt atgcagctct gctgctcggc gatgacggcc gaggcgctca agctctccaa
     3181 cccggcgagt gtcttctccc gctccaccga ggcgcacaac caggacaagg tgagcatggg
     3241 ctctattgcc gcccgcgatg ccagatccat catcgagtta gcgcagaacg tcgcggctat
     3301 acacgtcctc gccgtctgcc aggcactaga cctgcgcggc gtacagaagg cgagcccgaa
     3361 aaccagggcg gcgcacgaac tcgtgcgctc tcgcgtgcct ttcctggatg gcgaccggcg
     3421 aatggacacg gatatcaagg agatcgttga cctcatccgg tccggcgagc tctcgaagag
     3481 gaccctcccg acctgagcgg tcctcagttc gactcgtgct gtagcgaagg tcatggtcgg
     3541 ggaagttggc aatatcgtgt agcagttgat caagaagagg cgaaggctat gctacacggc
     3601 atgtgcggtg caagcacgcg gaggtggtga ccatgttgga cgcaaagaag ccctggctct
     3661 cgatctacgg agataggctc tcgggtgagc ccttgggcgg ttcgctgacg gagtttctag
     3721 aagaggcggt agagaagtac agggctaaca cggcgctgac tcggggtgag cagaagatat
     3781 cttacggcga gcttctggat ctgaccgaga ctttcgccgc cgcgctctac gaagccggag
     3841 tacggaaggg cgaccgtgtg gggctgatgc ttcccaattg tccagaatac gtcgtcgcgt
     3901 tcttcgggac gatgaggatc ggggccacgg ccacgcaggt caacccggtc tacgttggcc
//
LOCUS       DATMJA010000282         6768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_96228, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000282 DATMJA010000000
VERSION     DATMJA010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96228"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VNT12_06670"
     CDS             complement(<1..529)
                     /locus_tag="VNT12_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWI45837.1"
                     /translation="MTPPAEMSGQHETARQQSRAAWGAVARGWYAQREELWKVSRPVS
                     EWMVRRLDPQPGDTVLELAAGLADTGLMAARLVGEAGRAIVTDFTPEMVAAARRRAEE
                     LGVQNAEFRLLDAERMDLKTDIVDGVLCRWAYMLMIDPPAAFAETRRVLRPGGRLTFS
                     VWAARERNPALSLVGG"
     gene            1180..2286
                     /locus_tag="VNT12_06675"
     CDS             1180..2286
                     /locus_tag="VNT12_06675"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWI45838.1"
                     /translation="MSVIQPSTSSVTPPQAQPTEGSPARATQSSEQPSAEGSKETNVY
                     AATTDTQVGDRLASIPERVYSPNVGDATVDVIDPETFEIVDHYAVGEIPHHVTPSWDM
                     KKLYVNNEASSSLTVIDPKTGKPTDTVSVPYPYNLYFTPDGEKAIVVVERLQTLEFRD
                     PDTWRLLGSVYIPSPGVDHMDFSAEGDYLLASSEWGGVVTKVDTKEMETTGSIEVGGN
                     PVDVKLSPDGKVFYVANQGEGYGGVHVIDPRAMKQLKFIPTGAGAHGLYVSRDAKSLY
                     VANRLAGTISVIDFATRKVTATWEVGGSPDMLQLSPDGTQLWYADRYNGTVSVVNARS
                     GKLIHRIAVGYCPHGLTYFPNVGRFSLGHNGVYR"
     gene            complement(2513..5173)
                     /locus_tag="VNT12_06680"
     CDS             complement(2513..5173)
                     /locus_tag="VNT12_06680"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HWI45839.1"
                     /translation="MVAGTGDAEHGGGTGATPLEERVRRLEKDVEELREQVRTHARAQ
                     TQVRAIAETQNGARVETPKETPGEAATPTEERPAQSEIVSSPFAEATRNPVRHRGGNV
                     RERGPLLGLFPDGLPPATWWVARAGVVLLLVGVSFLLRMGVEQGWLTPAVRVAGGVVI
                     GGVLSAFGLLARTSRPAYSQLLVGGGVVAFYLSAFGAWSVWQLVPYEAAFAFCVLTTA
                     FAFAAAVRLSAEWLALLGVTGGYATPFMLLSPDSNVPGLVAYSLLLLGGYLTVYVLRG
                     WRPVYGTAIFGIWAILAVSDLGARAYTTSTEGVLPADAAWSVSMGALIAWLTILFLTS
                     ARRYLLADAGASVSSENGRSGSGVMTEAAAASVAPLVALFFAWSAWPSENLSGVHLAS
                     LAVAMALVHLVAYAIVANKALLSSSSSVAGTSRGWQGNVSGQAYSGFFEVSALAGLLE
                     DRSTETSRESYGEPSRESGISLALRYARTQAFASLALLTLAVVFLLEGAALASALAVE
                     GALLAYLVRPAESDDAVMRLAGRRLITAKSWLLLTFAALYSLGTVAWRAADPFGATGA
                     GDDAAYLPFLNGDAVCLLVVVASLFFVGRMGEGADPSPWRGVAAGARLFGHLLLALGV
                     VSEFARSDLPSGASFAFLALYALALALTRFVSDRNMLTTGESHLPSPALWRPPSWVAS
                     WLDVGAVLCVIAPWLWERLDASGMWAAYPTAVSQALSEPGAHLANLVGVLVLFGVAGL
                     LFLWGDARRGSSTAWEDERLLPSAAVLLLAASTFFALYWTWTVLDPLLGGSALVSVAW
                     GVLSAALLAGPALLLAPSPRETGSAGLIYWSSKVGYTVLALVVAKLFLYDLAAVDPIL
                     RILLFCGFGTAFLLAAYLFGGERSRTRSDT"
     gene            complement(5307..5933)
                     /locus_tag="VNT12_06685"
     CDS             complement(5307..5933)
                     /locus_tag="VNT12_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWI45840.1"
                     /translation="MRKLIVSNMMSLDGYYEGPGKNVMVLFDYRRHYPTDESFDAYNA
                     ERLRAADTLLLGRTSYDGFKGFWPSVADDPNATPLQREISRLNNTIDKVVISDTLTSK
                     ETEPWLNTRIIGRAEAHEQLAELKGRVGRDILVFGSRTLWSDLLANNLVDELHLMISP
                     VVLGAGTSLFDGQPAVSLGLVDTRMWDGSGNVLLRYEVRHQNPKAPVL"
     gene            complement(5977..6732)
                     /locus_tag="VNT12_06690"
     CDS             complement(5977..6732)
                     /locus_tag="VNT12_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45841.1"
                     /translation="MLLENKNAVIYGAGGAIGCAVARAFAREGAKVYLTGRTLESLEV
                     VAEEIRSTGRVAERAQVDAIDEQAVDQHADAVAASAGGIDVSLNLISVGDVQGTPLAE
                     MPLKDFERPVITAVRTQFLTSRAAARHMISQGSGVILFFGGYGDPAPDYYLGGFQVAL
                     NAIESLRRQLASELGGHGIRAVTLQTGGVPETIPESFAGREEITEGIVGQTMLGRAAT
                     LEDVGNVAAFAASDHARTMTATALNISCGAIVD"
BASE COUNT         1374 a         2166 c         2057 g         1171 t
ORIGIN      
        1 ccccgcccac gagcgagaga gccgggttgc gttcgcgggc ggcccagacg gagaaagtta
       61 gccgcccccc cggacgcagc acgcgccggg tttccgcgaa agccgcaggg gggtcaatca
      121 tcagcatgta ggcccagcga cagaggacgc cgtcgacgat atccgtcttg aggtccattc
      181 gctcggcatc gagtagccta aactccgcgt tctgcacgcc cagctcctcc gcccgccggc
      241 gagctgcagc gaccatctcg ggcgtgaaat ccgtgactat ggcacgccct gcctcaccca
      301 ccaagcgtgc cgccatgagc ccggtgtcgg ccagaccggc ggccagttcg agcacggtgt
      361 cccccggctg cggatcgagc cttctgacca tccactcgct gactggacga gaaaccttcc
      421 agagttcttc gcgctgagcg taccaaccgc gagccacggc tccccaggcg gccctgcttt
      481 gctggcgtgc cgtttcatgc tgccctgaca tctcggcagg cggtgtcatg tcactctccc
      541 ttttgcgtct cgctcgtgta ccaagataga gagccgacac attcggcgca atcggtttgc
      601 attcagaccg ggttttgtta cacctccaat cctcgcgcca ccttcactgc tcagatacgc
      661 ggcggcgtta gcgggggccg ttgtgcgacc gactccgcgt cggtcgacgc cgattgcgac
      721 aacggcgcta cccttcatgg tatgcctcgg ccctggagga gcgaccacgg tgacgtttga
      781 cagcgcaggg tgggcccgcg aggaggcgga ggtagggtag gtgtcctcga ctcccacagt
      841 agttgcccga ggtatggtgc cgacctcctg aacctttctt agatccgaaa tatggtgcta
      901 caaagcaatc tcacaggcat gtgtagtatt tctttgtcgt accgccgcat cgccaagctt
      961 tatagtgagc caagatctgt gtagacaaag ctgggagact gcgctacctt tgctccaatg
     1021 tatgggaggg gaagaaagaa ctctacaaag cttctccatc cccgtctcga tcccctcaga
     1081 aagcgtcttt accactactg gttgttgccg gctgtcctgg tcgtggtgtt tctgtgcggc
     1141 ctgcgattct cctctcatca ggtggtccac cacagggtta tgtcggtgat ccagccctct
     1201 acgtcttcag tgacgccacc tcaggcgcag ccgaccgagg gatcacctgc tagggctacc
     1261 cagagcagcg agcagcctag cgcagaaggc tcgaaggaga caaacgtcta cgccgcaacg
     1321 acggacacgc aggtaggtga tcggcttgcc agcatccctg aaagggtcta cagcccaaac
     1381 gttggggacg ccacagtgga cgtcatagac cccgaaacct tcgaaatcgt ggaccattat
     1441 gcagtaggcg agattcccca ccacgtaacc ccctcttggg acatgaaaaa actctatgtc
     1501 aacaacgagg cttcgagctc gcttacggtg atcgacccca aaaccggcaa gccgactgac
     1561 accgtctcag tcccctaccc ctacaacctc tacttcactc cagacggtga gaaggcaatc
     1621 gtggtcgtcg agcgcctgca gacactcgag ttccgcgacc ccgacacgtg gagactgctg
     1681 ggcagcgtct acatcccgtc ccccggcgta gaccacatgg acttctcagc ggagggcgac
     1741 tacctgctcg ccagcagcga atggggcggg gtggtgacca aggtggatac caaggagatg
     1801 gagaccacgg gttcgatcga agtcggcggg aatcctgtag atgtaaaact ctcgcctgat
     1861 gggaaggtgt tctatgtagc gaaccagggg gaaggctacg gcggcgtgca cgtgatcgat
     1921 cccagggcca tgaagcaact aaagttcatc cccaccggcg ccggagccca cggcctgtac
     1981 gtttccaggg acgcgaaatc cctctacgtc gccaaccgcc tcgcaggaac catctcagtg
     2041 atagacttcg cgacccgcaa ggtaaccgcc acgtgggagg tcggcgggag tcccgacatg
     2101 ctccagctct cgcccgatgg aacacagctc tggtacgccg accgttacaa cggcaccgtc
     2161 tccgtggtga acgctcgcag cgggaagttg atccacagga ttgcggtcgg ctactgcccg
     2221 cacggcctaa cctactttcc gaacgtcgga cgcttcagtt tagggcacaa cggcgtctac
     2281 cgctgatctt cctcggctag aggaaggagc cgacgaccga gttcaccttg accgcctcgg
     2341 ctagctgttc ttggcggatg ctaggctgtc aggattgacg ctgcgacttt gcgtcttata
     2401 ggagcgccgc tctcgcgaac tgaggggtgc gctttctccg aagacctttc ccaagaactg
     2461 ctcgaacaca ttccgaaaag ccctacaaca cacgaatgcg tgttttcggc aatcaggtgt
     2521 cggaacgggt tctactcctt tcgccgccga acaagtacgc cgcgagaagg aacgccgttc
     2581 cgaagccgca gaacaagagt atgcgcagga tgggatccac cgcggcgagg tcgtagagga
     2641 acagcttagc gaccaccagt gcgagcacgg tgtagcccac tttggaagac cagtagatta
     2701 gccccgccga accagtctct cgtggtgagg gggcgagcag gagggcggga ccggcgagga
     2761 gtgcggcgga tagtacgccc cacgcgacgc taacgagcgc gctgccgccc aagagtgggt
     2821 caagaaccgt ccacgtccaa tacagcgcga agaaggtgga agccgcgagc aacagcaccg
     2881 ccgccgaggg cagcaggcgc tcatcctccc acgcggtcga ggagcccctg cgggcatcgc
     2941 cccacaagaa gaggagccca gcaacgccga acagcacgag gacgcctaca aggttggcga
     3001 gatgggcacc gggctcggac aacgcttggg agacggcggt gggataggcc gcccacatcc
     3061 cgcttgcgtc aaggcgctcc cacagccatg gcgcgatcac gcaaagcact gcgccgacat
     3121 cgagccaaga tgccacccac gacggcggac gccatagcgc aggcgagggc aggtgcgatt
     3181 cgcccgtggt cagcatgttg cgatcggaga cgaatcgcgt gagcgccagc gcgagtgcat
     3241 agagcgcgag aaaagcaaaa gaggcaccgg aagggaggtc cgagcgggcg aattccgaga
     3301 cgacacccaa agccagcagc aggtgaccga acaggcgggc tcccgcggcc acgccgcgcc
     3361 agggcgaggg gtccgccccc tctcccatcc ggccgacgaa gaacagggag gcgacaacca
     3421 caagcaagca gacggcgtcg ccgttcagga agggcaggta ggcggcatcg tcccccgccc
     3481 ccgtcgcgcc gaacgggtcg gctgcgcgcc acgccacggt gcccaaagag tagagcgcgg
     3541 cgaacgtcaa gaggagccag gacttcgcgg tgatgaggcg gcgtccggcc agcctcatca
     3601 ccgcgtcgtc actttcggca ggacgcacga ggtaggcgag aagcgcgcct tccacagcga
     3661 gagcgcttgc cagcgccgcc ccctcgagga ggaacaccac ggccagcgtg aggagggcta
     3721 aggaagcgaa cgcctgcgtc ctggcgtaac gcagtgcgag ggagataccg ctttccctag
     3781 aaggctcccc ataagattca cgagacgttt ccgtcgagcg gtcttccagg agccctgcaa
     3841 gagcggatac ctcgaagaac ccactgtacg cctgcccgct gacgttgccc tgccaccccc
     3901 tggacgtacc ggcgacggag gaagaagacg aaagaagggc tttgttagca acgatggcat
     3961 aggcgacgag gtggacaagc gccatcgcca cagcgaggga ggcgagatgg acgccggaga
     4021 ggttttccga tggccacgcg ctccacgcga aaaacaaagc gacgagtggg gcgacgctgg
     4081 cggcagccgc ctccgtcatg actccactac cgctcctccc gttttccgaa gagacacttg
     4141 ccccggcgtc cgctagcagg tagcggcggg cgctggtgag gaagagtatc gtcagccagg
     4201 cgatgagcgc ccccatggac accgaccatg cggcgtcggc gggcaatacg ccctctgtag
     4261 aggtggtata ggcgcgcgcg ccgaggtcgg acacggcgag aatcgcccat atgccgaaga
     4321 tggcggtgcc atacaccgga cgccaccccc tcaggacgta gaccgtcaaa tagccaccga
     4381 gtagcagcag ggagtaggcg accaggcctg gcacgttgga gtcgggggag agcagcatga
     4441 acggcgtcgc gtagccgccc gtgactccaa gcagcgcgag ccactccgcg ctcagacgca
     4501 ccgccgccgc gaatgcgaac gcagtggtga gcacgcagaa ggcaaatgcc gcctcgtagg
     4561 gcacgagttg ccacacgctc cacgcgccga aggcggaaag gtagaacgcc acgacaccgc
     4621 cgcccacgag caattgcgag taggcgggac gggaggttct ggcaagcagc ccgaaggcag
     4681 agagaacgcc gccaatgacc acgccgcccg ccacgcgcac ggctggcgtg agccatcctt
     4741 gctcgacgcc catccgcaag aggaaggata cccccacgag aagcaggacg acgccagctc
     4801 tcgctaccca ccacgttgcg ggcggcagcc catcggggaa cagccctaag agcggtcccc
     4861 tttcccggac gtttccaccc ctgtgccgca cgggattgcg ggtggcttcg gcgaaggggg
     4921 aggagacgat ttcactttgc gcggggcgct cttcggtggg agtcgccgcc tcgccaggag
     4981 tttccttagg cgtttccaca cgtgccccgt tttgcgtctc tgcgatagcc cgcacttgcg
     5041 tttgcgcccg cgcgtgggtt cgtacctgct ctcgcagttc ttccacgtcc ttttccaacc
     5101 gccgcacgcg ctcttcgagg ggcgtcgcac cggttccccc gccgtgttct gcgtccccgg
     5161 ttcccgcgac catctcacct ccgtccgacc gtttacttac aacgtaagtc cattttagca
     5221 tatacagggg tagctctgca attggggact tagaccttcc gccgataggt cggcagtgct
     5281 tcggggcagg ggtctgtcgg atagaactag agcaccggcg ccttagggtt ttggtggcgg
     5341 acttcgtacc gtaggagtac gttccctgag ccatcccaca tgcgtgtgtc gacgagtcca
     5401 agcgaaactg ccggctgacc gtcgaatagt gatgtgcctg cgcccaatac aaccggactg
     5461 atcatcaggt gtagttcgtc gaccagattg tttgccagca agtcgctcca gagcgttcgg
     5521 ctaccaaata ccaaaatgtc tctgccgact cggcctttga gttcggcaag ctgctcgtga
     5581 gcctctgcgc ggccgatgat gcgggtgtta agccatggct ccgtttcttt tgaggtcagt
     5641 gtatctgata tgaccacctt gtctatggtg ttattgagcc gtgatatctc ccgttggagg
     5701 ggtgtggcgt tgggatcgtc tgccacggac ggccagaaac ccttgaagcc atcgtatgaa
     5761 gtacgtccca gcagcagcgt atcagccgca cgcagccgtt cggcgttgta ggcgtcgaag
     5821 ctttcgtccg tcgggtagtg ccgacgatag tcgaacagga ccatgacatt cttacccggc
     5881 ccctcgtaat agccatccag cgacatcatg ttgctgacga ttagtttacg cattacacgc
     5941 ccccttatgc atctatcgag acaagagcac ctccgcctag tctacgatcg cgccacagct
     6001 gatgttgagg gctgtcgcgg tcatagtgcg ggcatgatct gaggcagcga aggcggccac
     6061 attgccgaca tcctcaagcg tcgctgcccg ccccagcatc gtctgcccaa cgattccttc
     6121 ggttatttcc tcgcgaccag cgaagctctc cgggatcgtc tctggcaccc cgcccgtctg
     6181 cagcgtcacg gcgcggatac catgccctcc gagctcggag gctaactgac ggcgcaggga
     6241 ttcgatggcg ttgagtgcaa cttggaatcc tcccaggtag tagtcgggcg ccgggtcccc
     6301 gtagcctccg aagaagagaa tcacccctga tccctgagag atcatatgac gtgctgccgc
     6361 cctggaggtc aaaaactgtg tcctcacggc ggtaatgacc ggccgctcga agtctttgag
     6421 gggcatctcg gcgagcggcg tcccctggac atccccgacc gagatgaggt tgagggagac
     6481 atcgatccct ccagcgctcg cggccacagc gtcagcgtgt tggtcgacgg cctgctcgtc
     6541 gatggcatcg acttgcgccc tctcggccac tcttccagtg gagcggatct cctcggccac
     6601 cacctcgagg ctctcaaggg tacgaccggt gaggtaaacc ttcgctccct cgcgggcgaa
     6661 ggcgcgcgcc accgcgcaac ctatagcccc tcccgccccg tagatcacgg cgttcttgtt
     6721 ctcgagcaac acgtccgtcc tcctctgcta gcaacgacat cgacgtcc
//
LOCUS       DATMJA010000283         3314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_37509, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000283 DATMJA010000000
VERSION     DATMJA010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37509"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VNT12_06695"
     CDS             <1..1038
                     /locus_tag="VNT12_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWI45842.1"
                     /translation="GRPMEGKTRRPVTVVTGFLGSGKTTLLARVLSAPSMANTAVLVN
                     EFGEVGLDHHLLRRVDERTVLLGSGCVCCTTREDLVGALMELLDMDQRGEIPRLDRVV
                     IETTGLADPAPILHTIYANPILQHHFSVDLVLTTVDAVNGALHLDRNPESVKQVAAAD
                     KVVVTKTDIAEPGIVPGLLSRLGKINPSAWTCEAAFGEVDVHELFRPDGAGTHAAPAA
                     FTAESGDPHDVGDTHSVSVTFDGAIDWTAFGIWFSMLLNARGEDVLRVKGLLDVGEAG
                     PVVINGVQHIIHPPEHLGEWPDEDRRSRIIFITRYIHPEELLDSLLAFRGLLGARPLP
                     LHAAEQRVPFL"
     gene            complement(1105..1551)
                     /locus_tag="VNT12_06700"
     CDS             complement(1105..1551)
                     /locus_tag="VNT12_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45843.1"
                     /translation="MIGRRVGDPPRTSEHILRMDPTLDLERRRLLISAALTTKARRHL
                     GSSLAASVSVGELLDLACGVSEGAGTIERAYSLCFDPPYPGVTAGPQSVGGTPRLMLA
                     CSVRDRVGTPIGVVFTTLIAGRPPQVSVAPAGASIPEGWRSPGDFH"
     gene            complement(1548..1967)
                     /locus_tag="VNT12_06705"
     CDS             complement(1548..1967)
                     /locus_tag="VNT12_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HWI45844.1"
                     /translation="MTLTIGTGPFGDQGDKTFNFEVQAPRDHVLFFEDSPRRVRVMFG
                     GQTVADSRRVKLLHEQGHLPVYYFPREGVRMDLLQATNHTTQCPFKGEAFYWSVRVGD
                     RVAENAVWGYPDPIDSAPPLADYLAFYWNEMETVSLE"
     gene            2248..2949
                     /locus_tag="VNT12_06710"
     CDS             2248..2949
                     /locus_tag="VNT12_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI45845.1"
                     /translation="MSNESEDVIRVAVVDDQRLFTRGISGLVDMLPGVEVVGVAYDGE
                     EAIALCRKEEPDVVLMDISMPKMDGISATREIKALLPQTAVVILTGHEEDEHVFEGIK
                     AGAQGYLLKDSEPEDLSRAIHTVYTGDTIVAPDLAQKMLSTFEGGRPGGAHLAPPLTE
                     RELEVIKALGQGMSDRQIAHALGISEKTVRNHTSNIYRKLHIFDRTQAVIYAVREGVI
                     DVGDLEYRPPREPLP"
     gene            complement(2897..>3314)
                     /locus_tag="VNT12_06715"
     CDS             complement(2897..>3314)
                     /locus_tag="VNT12_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI45846.1"
                     /translation="RPFLESVESLVELNQQAPPEREIRLVVEDGFPVGLPGKESVEVL
                     RVLQEALTNARRHSEARNVEVGLRTDDEAILIEVADDGQGFDPGSARTGIGLSAMRER
                     VEGLGGKIEISSRPGEGTEVMVRVPVGGGTPDPRRL"
BASE COUNT          630 a         1073 c         1016 g          595 t
ORIGIN      
        1 ggtaggccca tggagggcaa gaccaggcgt ccggtgacgg tcgttaccgg tttcctcggc
       61 agcggcaaga cgacccttct cgcgagagtg ctctcggcgc cgtcgatggc caacacggcc
      121 gtcctcgtca acgagttcgg cgaggtgggg ctcgaccacc acctcctgcg ccgggtggat
      181 gagcgcacgg tcttgctcgg gagcggttgt gtctgctgca ccacccggga ggacctcgtc
      241 ggagcgctca tggagctcct cgacatggac cagaggggcg agataccgcg gctggatcgc
      301 gtcgtcatcg agacgaccgg cctggcggac ccggccccta tcctccacac catctatgcc
      361 aaccccatcc tgcagcacca cttctctgta gacctggtac tcaccaccgt ggacgcggtc
      421 aacggcgccc tgcacctcga ccgcaacccg gagtcggtca agcaggtcgc ggcggcagat
      481 aaggtcgtcg tgaccaagac ggacatagcg gagccgggca tcgtaccggg cctgctgtcc
      541 cggctgggca agatcaaccc gtcagcctgg acctgcgagg cggctttcgg cgaggtggac
      601 gtccacgagc tctttcgccc ggatggcgcg ggaacgcacg cggcgccggc ggcctttact
      661 gcggagtccg gcgatcctca cgacgtgggc gacacgcact ccgtctcggt gacgttcgac
      721 ggggccatcg actggacggc gttcgggatc tggttcagca tgctgctgaa tgcccggggt
      781 gaggacgtgt tgcgtgtcaa aggacttctt gacgtcggcg aggcagggcc cgtcgtcatc
      841 aacggcgtgc agcacataat ccatccgccg gagcacctcg gcgagtggcc cgacgaggac
      901 cgccggtccc ggataatctt catcaccagg tacatccatc ccgaagagtt gttggactcc
      961 ctgctcgctt tccgcgggtt gctcggagcg cgacctttac ccctccacgc cgccgagcag
     1021 agagtaccgt tcctctagtc gcttgaagga tctcgcaaaa tggtcgtggg accatcagcc
     1081 acgcgggtcg agtggcgtgg ggtctcagtg aaagtcgccc ggcgaccgcc aaccttcggg
     1141 aatgctcgct ccagcaggcg ccaccgacac ctggggaggg cggccggcga tgagcgtggt
     1201 aaagaccaca ccaataggag ttccgacccg atcgcggacg ctgcacgcga gcatgagcct
     1261 gggggtcccc ccgacgctct ggggtccggc cgtgacgccg gggtagggcg gatcgaagca
     1321 gagactgtac gcgcgctcga tcgtgcccgc tccctcgctc accccgcagg ccagatccag
     1381 gagttccccg acgctaacgc tggcagcgag gctgcttccc aggtgccgcc gggccttcgt
     1441 ggtcagcgcc gcggatatga ggaggcgtct cctctcgagg tcgagggtag gatccatgcg
     1501 caagatgtgc tcgctcgtac gtggcgggtc gcccactctc ctgccaatca ttctaacgat
     1561 accgtctcca tctcgttcca atagaacgcc aggtaatcag caagcggcgg agcagagtct
     1621 atcgggtcag ggtagcccca cacggcattc tccgcgacgc ggtcgccgac cctcacagac
     1681 cagtagaacg cttcaccctt gaaagggcat tgcgtcgtgt ggtttgtggc ctggagaagg
     1741 tccatccgca cgccctctct ggggaagtag tagactggca ggtgtccctg ttcgtgcaag
     1801 agctttaccc gcctgctgtc agctacggtc tggccgccga acattacccg cacccgtcgt
     1861 ggagaatcct cgaagaacag cacgtggtct ctgggcgcct gcacctcgaa attgaacgtc
     1921 ttgtcgccct ggtcgccgaa cggtcccgtc ccgatcgtaa gggtcatcgc tcctctcctc
     1981 tctagcggcc gcagctcgac aaaagcagct ggcagacccg catcttccgg cctttatagt
     2041 acacgcgcac aatgggtcgg gtgacctact cagaattacc cggcttggcg tggtccaaat
     2101 atccgacgat aacgctgccc gtgcggacga tcattcgaga gacagggcac tcagtatctc
     2161 caccggttca tccgtgacac gccaggtgga cagcggatac agttagagtc gtactggact
     2221 tcgatgcggt aaaggggcgt gaggtttttg agcaacgaaa gcgaagatgt catccgcgtg
     2281 gcggtagtgg acgaccagcg gctcttcacc aggggtatct ccggcctcgt agacatgttg
     2341 cccggcgttg aggtggtggg tgtagcctac gacggcgagg aggccatcgc gctctgccgc
     2401 aaggaagagc cggatgtcgt cctgatggac atctccatgc cgaaaatgga cggtatcagc
     2461 gccacgcgcg agatcaaggc tcttctgccg cagaccgcgg tcgtcatcct cacgggccac
     2521 gaggaagatg agcacgtctt cgagggcata aaggcgggcg cgcaaggcta tctgctaaag
     2581 gactcagaac ccgaagatct ctcgcgtgcg atccacacgg tctacacggg ggatactata
     2641 gtcgccccag acctcgccca aaagatgctc agcaccttcg agggcggcag gccagggggg
     2701 gctcatctcg ctcctccgct gaccgagagg gagctcgaag tcataaaggc cctgggccag
     2761 ggcatgagcg acaggcagat agcccacgct ttaggtatct cggagaagac ggtcaggaac
     2821 cacacttcca acatctacag gaagctgcac atcttcgaca gaacccaggc ggtcatctac
     2881 gccgtgaggg aaggcgtcat agacgtcggg gatctggagt accgcccccc acgggaaccc
     2941 ttaccataac ctctgtaccc tcaccgggcc ggctgctgat ctcgatcttc cctcccagac
     3001 cctcgacacg ctcgcgcatg gccgagagcc cgataccagt cctggccgac ccaggatcga
     3061 atccctgtcc atcgtcggcg acctctatga gaatcgcttc gtcatccgtc cgcaacccca
     3121 cctcgacatt ccttgcttcg gagtggcgcc tggcgttggt aagggcctcc tgcagtacgc
     3181 gcaacacctc tacgctctcc tttccaggga gacctacagg gaatccatcc tcgacgacta
     3241 accgtatctc gcgctctggt ggtgcctgct ggttcagctc caccaacgat tcgacggact
     3301 ccaagaacgg ccgc
//
LOCUS       DATMJA010000284         6325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_333808, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000284 DATMJA010000000
VERSION     DATMJA010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333808"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..686
                     /gene="hpt"
                     /locus_tag="VNT12_06720"
     CDS             135..686
                     /gene="hpt"
                     /locus_tag="VNT12_06720"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWI45847.1"
                     /translation="MSSMMPDVKEVLITSQEIEEKVREIGARITEDYRGENPLLIGVL
                     RGAVVVMSDLMRNIDLQCELDFMDISSYGSGTSSSGVVRILKDLEEDITDRHVLIVED
                     IIDTGLTLSYLRRSLLARGPASLEICALLSKPSRRRTDLDVKYLGFDVPDEFVVGYGL
                     DYAGAYRNLPDICILKPEVFATG"
     gene            764..2671
                     /gene="ftsH"
                     /locus_tag="VNT12_06725"
     CDS             764..2671
                     /gene="ftsH"
                     /locus_tag="VNT12_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWI45848.1"
                     /translation="MGRILRSGGLLYFVILIVLAVVLVNMLSRGNPDVEELNSQQWKQ
                     AVQDKSFVTDLPKTSENYLTILDEDQTVTGELKTGNGDTKKFEYSYTQMTDVASQLDK
                     AGVAYSVDPQNTGFWLTLLGTLAPILLIVLFFILFMSSMQGGGNRVMSFGKSRARRMT
                     KDQPKVTFSDVAGADEAVQELTEIKEFLEAPQKFQKLGARIPKGALLVGPPGTGKTLL
                     ARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRQ
                     RGAGLGGGHDEREQTLNQLLVEMDGFDSKSGIIMLAATNRADILDPALLRPGRFDRQI
                     VVDRPDLPGREKILRVHTRGKPLGPDVQIGTLARGTPGFTGADLANLVNEAALLAARH
                     DKNQVDMSEMEEAIDRVIAGPERKTRLISDKEKEITAYHEGGHAIVGALLPDADPVHK
                     ITIIPRGQALGVTMSLPTEDRFMMSRGQLMAQLAMMLGGRAAERVVFDEITTGASNDL
                     ERVTQTARQMVTRFGMSEKLGPMALGHQQGQVFMGRDFNNQPDYSDEIAFQIDKEIRR
                     IVDESYDTAEDILVRNRELLDKLSAELIEYETVDAEHLKRLIEEYAVDNISPNGAVPD
                     SDGRYRGSYQD"
     gene            2672..3532
                     /gene="folP"
                     /locus_tag="VNT12_06730"
     CDS             2672..3532
                     /gene="folP"
                     /locus_tag="VNT12_06730"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWI45849.1"
                     /translation="MGRTARNTDRAGTLTAGPGPFIVGILNVTPDSFSDGGDFLNPEA
                     AAEHAATMLDEGADVLDVGGESTRPGSDPVSQEEEIRRVVPVLERILSVRPEAVISVD
                     TYRSGTATAALEAGASLVNDVTALRGDPRMVSVIEEAACPVILMHMQGEPKTMQKEPH
                     YEDVVGEVRDFLAERAEYAVAAGVRPENIIVDPGIGFGKNLEHNLALLHNLDAIVDLG
                     FPVLIGASRKSFIGRITGVQLARDRVSGTVATTVLAYERGATFFRVHDVRANREALAV
                     AEAVLHAPRR"
     gene            3529..4032
                     /gene="folK"
                     /locus_tag="VNT12_06735"
     CDS             3529..4032
                     /gene="folK"
                     /locus_tag="VNT12_06735"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWI45850.1"
                     /translation="MSRAFLSLGSNLGDRLAYLRSVLAALGRGPRMRITGTSKVYETV
                     PVEVEEEQPDYLNCVVELECGIPAIELLRFCQGVEASLGRERGEAGEKAPRTVDIDVL
                     LFGEVTIMEPDLRVPHAGVTRAFNLRGLADIDMDLYIPGHGTVLELLGRAEEGGILTF
                     GEAEELC"
     gene            4032..4796
                     /locus_tag="VNT12_06740"
     CDS             4032..4796
                     /locus_tag="VNT12_06740"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWI45851.1"
                     /translation="MAVDVGNTQTVLGLIDGEELRGQWRIATEAHRTADELAIFFAGL
                     LALEGLSPDEVSAMIVSSVVPGLTRAYRNLASGVFGVPYYGVSTRMETGLDNRYDDPE
                     EVGADRIVNAVATGRHYGFPAIIVDIGTATTVEAVDGKACYLGGAILTGLYVSLDALV
                     SKTAKLPSVDLEEEPPRVIATNTPDSIRSGFIYGYAGAIDALIRRSRDELGEDGVKVV
                     ATGGPAGVIVPYCREIQEFDPDLTLKGLLALYEMNA"
     gene            4928..5416
                     /gene="greA"
                     /locus_tag="VNT12_06745"
     CDS             4928..5416
                     /gene="greA"
                     /locus_tag="VNT12_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWI45852.1"
                     /translation="MSDKNQELVTPLGLEKLQQELAYLTETRRKEVANRIRQAREFGD
                     ISENSEYDEAKNEQGLLERRIGELQRRVRNVRVVDPSEAEEDAVDLGTRVTLRAVGRE
                     GQERTFQIVGANESDPTSGKLSHASPVGRAVLKRKVGEKVMVSTPRGATEYEIVNVEA
                     AS"
     gene            5474..>6325
                     /gene="lysS"
                     /locus_tag="VNT12_06750"
     CDS             5474..>6325
                     /gene="lysS"
                     /locus_tag="VNT12_06750"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWI45853.1"
                     /translation="MSETGRSTGIHIPGWAERVADSLGAGPHVVVSGISVSGNIHAGN
                     LREVLVAEAVANALRQRGEEVRLIFHADTIDPLRKIAPGIPKSYEDYIGHSLSHIPDP
                     EGCHVSYAEHFLVPFEEALTEMEMDIEVLRSHELYEQGVYTSVTSEAIENTDELREIL
                     QEVTNRKMPENWSPYLPRAASGKLTGMRVLEHLPEQTSVVFADEDGWEDSADYSRGEG
                     KLGWRVELAARWKALGVTFEPFGKDHTSRGGSTDTADRMSREVFHYPVPGRYEYEWIG
                     LKGRGDMS"
BASE COUNT         1315 a         1894 c         2026 g         1090 t
ORIGIN      
        1 cacagaacgc ccgtcgtggt gggcgggagt ggcgaggtgg cctggatcgt gggcggcgag
       61 atcggcgagg ggtacaaggt aggcgagacg accgagaaaa ttctgaggct ggaggtggag
      121 agactctcgt gagtatgtcc agtatgatgc ccgacgtgaa agaggtgctc ataacctcac
      181 aggagatcga ggagaaggtg cgtgagatcg gggcgcgcat caccgaggat tacaggggcg
      241 agaatccgct cttgatcggt gtactgcgcg gcgcggtcgt ggtgatgagc gacctcatgc
      301 gcaacataga tctgcagtgc gagttggatt tcatggacat aagctcatat ggtagtggca
      361 cctcatcgag cggcgtcgtt cgcatcctca aggacctcga agaggacatc acagaccgtc
      421 acgtcctgat cgtcgaggac atcatagaca cggggctgac gctctcgtac ttgaggcgtt
      481 ccctgctcgc ccgcgggccc gcctcgctcg agatctgcgc tcttctctcc aagccttcca
      541 ggcggcggac ggacctcgac gtgaagtatc tcggcttcga tgtgccagac gagttcgtcg
      601 tcggatacgg gctcgactac gcgggagcct accgcaacct ccccgatatc tgcatcctca
      661 agcccgaggt cttcgcgacc ggctgaaaga aggcgcatcc cattcagttc gcaggtagta
      721 taattcccat gtcctagacg cacttcatgg aaggtaagaa gctttgggca gaatcttgag
      781 aagcggcggg ctgctgtact tcgtcatcct aatagtcttg gccgtcgtcc tggtaaatat
      841 gctgagccgg ggcaacccgg acgtggagga actcaattcc cagcagtgga agcaggcggt
      901 acaggacaag agtttcgtca ccgacctccc caagaccagc gagaactatc tgacgatcct
      961 cgacgaagac cagacggtaa cgggtgagct gaaaaccggc aacggggata ccaagaagtt
     1021 cgagtactcc tacacgcaga tgaccgacgt ggcgagccag ctcgacaagg cgggcgtggc
     1081 ctattcggtg gacccgcaga acacgggctt ctggctcact ctgctaggca ccctcgcccc
     1141 gatcctgctc atcgtattgt tcttcatcct gttcatgagc tccatgcagg gtggcggaaa
     1201 cagggtgatg agcttcggca agagccgtgc ccgcaggatg accaaagacc agcccaaggt
     1261 caccttctca gacgtcgctg gcgccgatga ggccgtacag gagctaaccg agataaagga
     1321 gttcctggaa gcgccccaga agttccagaa gctcggggct cgtattccta aaggcgcgct
     1381 actcgtaggc cctcctggga caggcaagac gttgctcgcc cgcgctgtcg ccggggaggc
     1441 cggtgttccg ttcttctcca tctcgggctc ggacttcgtc gagatgttcg ttggtgtagg
     1501 cgccagccgc gtgcgcgacc tcttcgagca ggccaagcag aactccccct gcatcatctt
     1561 catggacgag atcgacgctg taggacgcca gaggggcgcg ggcttaggcg gcggccacga
     1621 cgagagagag cagaccctaa accagctcct tgtggagatg gacggtttcg actccaagag
     1681 tgggatcatc atgctcgccg ccaccaaccg cgccgatatc ctcgaccccg cgctcctaag
     1741 accagggcgt ttcgaccggc agatcgtggt cgaccgtccc gacctgcctg ggagggagaa
     1801 gatcttaagg gtgcacacaa gaggcaagcc ccttggcccc gacgtgcaga tcggaactct
     1861 tgccaggggg acccctggct tcaccggagc cgatcttgcc aacctcgtca acgaggccgc
     1921 gctccttgcc gcccgtcacg acaagaacca ggtcgacatg tcagagatgg aagaggccat
     1981 agaccgcgtg atcgcaggtc cggagaggaa gactaggctc atctccgaca aggagaagga
     2041 gatcactgcc taccacgagg gcgggcacgc catcgtggga gccctccttc ccgatgccga
     2101 ccctgtgcac aagatcacca tcatccctag aggtcaggct cttggcgtaa ccatgagcct
     2161 gccaacggag gaccgcttca tgatgagccg cggccagctg atggctcagc tggccatgat
     2221 gctaggcggt agggctgcag agagggtggt cttcgatgag atcacaacag gggcctcaaa
     2281 cgacctagag cgggtaacgc agaccgccag gcagatggtc acccgcttcg ggatgagcga
     2341 gaagctagga cccatggcct tggggcatca gcaggggcag gtcttcatgg ggcgcgactt
     2401 caacaaccag cctgactact cagatgagat cgccttccag attgacaagg agataagaag
     2461 gatcgtcgat gaatcctacg acacagcaga ggacatactg gtgcgtaatc gtgagctgct
     2521 cgacaagctc tctgcggagc tgatcgagta cgagaccgta gacgcagagc acctgaagcg
     2581 cctgatcgag gagtacgccg tggataacat ctcaccgaac ggtgccgtcc ccgactccga
     2641 cggccgctac aggggtagct accaggacta aatgggccgc acggcccgga acacagatag
     2701 ggccggaacc cttacagcgg gtcccggtcc tttcatcgtc ggcatcctga acgtcacccc
     2761 tgattccttc tccgacggcg gcgatttcct caatccggaa gccgcggcag agcacgccgc
     2821 taccatgctc gacgaggggg ccgacgtcct cgacgtcggt ggagagtcta cccgccccgg
     2881 ttcggaccct gtctcgcagg aggaggagat acgtcgcgtc gtccctgtac tcgagagaat
     2941 actctccgtg cgtcccgagg cggtgatctc cgtcgacacc taccgctccg gcacggctac
     3001 ggccgccctc gaagccggcg caagtctcgt caacgacgtt acagcgctcc gtggcgaccc
     3061 ccgcatggtc tctgtaatag aggaagcggc gtgcccggtg atactgatgc acatgcaggg
     3121 cgagccgaag accatgcaga aggaacccca ctacgaggac gtggtgggcg aggtcaggga
     3181 cttcctcgct gagagggccg agtacgctgt cgctgcaggc gtcaggcccg agaacataat
     3241 cgtggatccc ggcatcggat tcgggaagaa tctcgagcac aacctggcgc tcctccacaa
     3301 cctggacgcc atcgtagatc tgggtttccc tgtcctgatc ggagcctcgc gcaaaagctt
     3361 catagggagg atcacaggcg tgcaattagc gagagatcgc gtctccggca cggtggcgac
     3421 gacggtgctc gcctacgaac ggggcgcaac gttcttccgg gtgcacgacg tacgcgccaa
     3481 ccgcgaagcg cttgcagttg cagaagccgt gctccacgcc ccccgccggt gagcagggcc
     3541 ttcctgagcc tgggtagtaa cctgggcgac cgtctggcct acctgaggag cgtactcgcc
     3601 gccctcggtc gaggccctcg catgaggatc acaggtacgt cgaaagtcta cgagacggtg
     3661 cctgtcgagg tcgaggagga gcagccggat tatctgaatt gcgtggtcga gctggagtgt
     3721 ggcattcccg cgatcgagtt gctccgcttc tgccaggggg tcgaggcctc gctcggccgc
     3781 gagcgaggcg aagccggcga gaaggcccca cgcacggtgg atatagacgt gctgctcttc
     3841 ggagaggtga ccatcatgga acctgatcta cgggtgccgc acgcgggggt gacgcgtgcc
     3901 ttcaacctca ggggactcgc ggacattgat atggatctgt atattcccgg tcacggaacg
     3961 gtcctggagc tacttggaag agcggaagag ggaggaatcc tgacgttcgg ggaggctgaa
     4021 gagctttgtt gatggctgtg gacgtaggga atacgcagac tgttctgggg ctcattgatg
     4081 gcgaggagct gcggggacag tggcgtatcg ccacggaggc gcacaggacg gccgacgagc
     4141 tcgccatctt cttcgcgggg ctacttgctc tcgaaggtct aagccccgac gaggtgagcg
     4201 cgatgatcgt ctccagcgtc gtgcctggtc tcacccgtgc ctaccgtaac ctcgcctccg
     4261 gggtcttcgg ggtaccctac tacggggtgt ccacgaggat ggagactggc ctcgataacc
     4321 gctacgacga ccctgaggaa gttggggccg acaggatcgt caacgccgtg gcgacaggga
     4381 ggcactacgg gttccccgcg atcatcgtcg atatcgggac ggcgacgacc gtcgaggccg
     4441 tcgacggtaa agcatgctac ctcggaggcg ccatactgac aggcctctac gtttcgctgg
     4501 atgcgctcgt ctccaaaacg gccaagctcc ctagcgtcga ccttgaggaa gaaccgccga
     4561 gagtcatagc caccaatact ccagactcta tccgcagcgg tttcatctac ggctacgctg
     4621 gcgccatcga cgccctgatc aggaggtccc gcgacgaact gggcgaagac ggcgtcaagg
     4681 tcgtcgccac aggaggtcct gccggggtca tagtgcccta ctgccgcgag atccaggagt
     4741 tcgatcccga cctcaccctg aaaggcctcc tcgcccttta cgagatgaac gcgtgagagc
     4801 gttcattcag caagtcagcc agcacacgaa tgctgacaat gcggcggagc cgccgtgctg
     4861 aatcgctgcc tgcggcagcg attcctcgtt gacacccctt gcacccgccc ttataatctc
     4921 gcggcttatg agcgacaaga accaggagct cgttaccccg ctgggtctcg agaagctcca
     4981 gcaggaactc gcatacctga ccgagacgcg tcgcaaagag gttgcaaacc gcatccgcca
     5041 ggcccgtgag ttcggggaca tctccgagaa ctccgagtac gacgaggcga aaaacgaaca
     5101 gggcttgctg gagcgccgga tcggtgagct ccagcgcagg gtacgcaacg tgagggttgt
     5161 cgacccttcg gaggccgagg aagacgcggt cgacctcggt actcgggtaa cgctgagggc
     5221 cgtcggcaga gagggacagg aacggacgtt ccagatcgtc ggcgccaacg agtcggaccc
     5281 gacgagcggg aagctcagcc acgcctcccc ggtgggccgg gccgtgctca agaggaaggt
     5341 gggggagaag gtcatggtat ctaccccccg cggcgcgacg gagtacgaga tcgtcaacgt
     5401 cgaggcggcc agctagggcg ccgggagccc gcttctccaa agttccaacc accgaaccca
     5461 attggagtac cgcatgagcg agaccggcag atcaaccggc attcacatac ccgggtgggc
     5521 cgagagggtc gctgattctc ttggcgcggg gccgcacgtg gtcgtgagcg ggatcagcgt
     5581 ctctggtaac atccacgccg gcaacctgcg cgaggtcctc gtcgccgagg cggtcgcgaa
     5641 cgctctgcgg caacgcggcg aagaggtccg tttaatcttt cacgcagaca cgatcgaccc
     5701 gctgcgtaag atcgccccag gcatccccaa gagctacgag gattacatcg gccacagcct
     5761 ctcgcacata ccggaccctg agggctgcca cgtctcctac gccgagcatt ttctagtgcc
     5821 gttcgaggag gcgctcactg agatggagat ggacatagag gtgctccgct cgcatgagct
     5881 gtacgagcag ggcgtctaca ccagtgtaac cagcgaggct atcgagaaca cggacgagtt
     5941 gcgcgagatc cttcaagagg tcacgaatcg aaagatgccc gagaactggt cgccctacct
     6001 gccgcgggcg gcatcgggga agcttactgg gatgcgcgtc ctggagcact tgccggagca
     6061 gaccagcgtc gtcttcgcgg atgaggacgg ctgggaggac agcgccgatt actccagagg
     6121 cgagggcaag ctgggctggc gggtcgagct cgcggcccgg tggaaggcgc tcggcgtcac
     6181 cttcgagcct ttcgggaagg accacaccag ccggggcggc tcgaccgaca ccgccgatcg
     6241 gatgtcccga gaggtattcc actaccctgt gcccggccgt tacgagtacg agtggatcgg
     6301 gctcaagggt cgcggggaca tgagc
//
LOCUS       DATMJA010000285         4313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_689213, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000285 DATMJA010000000
VERSION     DATMJA010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689213"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..571
                     /locus_tag="VNT12_06755"
     CDS             203..571
                     /locus_tag="VNT12_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45854.1"
                     /translation="MEGFLQASLCGDMETLLELLSDDVTLYSDGGGKTRAALRPVYGA
                     DNVARFLTGILRNIPPGFAVRHTRVNGRPGLVGYFGDGSPQSVVSIEVAEGSIRAIRL
                     VVNPEKLGNVPPLEREEDSR"
     gene            731..1168
                     /locus_tag="VNT12_06760"
     CDS             731..1168
                     /locus_tag="VNT12_06760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWI45855.1"
                     /translation="MNDATQTLEPFVGQWAVLLTTYKRDGTPVGTAVNIAVQGDRAYF
                     RTWDTAWKLRRIRNNPEVEFAASTARGRPTAPAIGARARVLEGEGSARAGRLLARKYP
                     ALHGLLVPLVHRLRGNKTMHVELTPAEAPSQITSAPSGSHRPA"
     gene            complement(1642..3387)
                     /locus_tag="VNT12_06765"
     CDS             complement(1642..3387)
                     /locus_tag="VNT12_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWI45856.1"
                     /translation="MVEILLQKNVPAEMRDGATLMSNVYRPAGGGGYPVLLTRLPYGK
                     DLPSGASVLDPVTAAEAGYIVVVQDVRGRYRSEGKFVPFVKEYEDGYDTVEWAAKLPD
                     SNGSVGMYGLSYFGKTQWHAAVMRPPSLKSMVPGLTWGNHLNGVQMRGGVQELGLMQF
                     WAQTALTLDLLFRKYAGDRELIGEKLPEVIHTIDTLLAGGGYDVLPLTELPNPDELVP
                     FVEGGFGRGVHHEDWEYLNIEGKYENVDAPTFHIGGWYDCFIGETLRQYEAMKECSRE
                     AGLRPPHLLVGPWTHGDFGSTFGELDFGIGSSGMFLDYRGDLTDAHLRWFDATIKGDE
                     SALEGTPPVKVFVMGENRWRGYEEWQPPRARTEDWHLRSDGSLCREPGGARDEPARFD
                     YDPEDPVPTVGGPVLMAAIHGSGPRDQRSIESRPDVLVYTSPTLERPLTVLGSVYVTL
                     FAASSAPDTDFVARLVDVYPDGRSICVADGILRASARESYPAPGVVTPVAPSPIEPGE
                     VYEYVIDLWATGITFLPGHRLRVEITSSSHPRWERNLNTGESTLASSRTEVAHQTIFN
                     DAERRSRISLTVVEG"
     gene            complement(3387..4124)
                     /locus_tag="VNT12_06770"
     CDS             complement(3387..4124)
                     /locus_tag="VNT12_06770"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI45857.1"
                     /translation="MKVIGAGFGRTGTWSLKAALEVLGFGPCYHMTEVFAHPGHADFW
                     SSAWRGEPVDWEGVLGGYEATVDWPACTFYEELMERHPEAKVLLSVRDPEPWYESTRS
                     TIYELSMLLESSPIARLIFGLISLLVFGGFAGGRSSIVNDMIWQGTFDGRFEDKAYAI
                     EVFERHNEEVKRRVPSERLLVYEVTEGWVPLCEFLGVPEPEETFPHLNDATLLRRGTK
                     AVRTLATAVPVALALSIAAVLVLRRRA"
     gene            complement(4126..>4313)
                     /locus_tag="VNT12_06775"
     CDS             complement(4126..>4313)
                     /locus_tag="VNT12_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HWI45858.1"
                     /translation="VLREGESATEDELHTHLEVDFARFWLPDAYEFVEAIPMTATGKF
                     QKLKLREQFEGYTSPKS"
BASE COUNT          764 a         1398 c         1380 g          771 t
ORIGIN      
        1 cctggtgttg ctcgagagcc tcaccccggt cgagcgggcc gtgttcttgc tcagggaggt
       61 cttcgactac gagtatgcgg agatagcagc cctcgtcgga aagagcgagg ccaactgccg
      121 ctagatctca cgccgcgcca ggcagtcggt ggcggccagg cgaccccgct tcgagagctc
      181 cccgcagcag gaggaacgcc tgatggaggg tttcctgcag gccagcctct gcggcgacat
      241 ggagaccctg ctcgagctgc tctccgacga cgtgaccctc tactcagatg gtggagggaa
      301 gacacgggcc gcgctgagac ccgtctacgg cgccgacaat gttgcccggt tccttaccgg
      361 aatactgcgc aacatcccgc ccggcttcgc cgtaaggcat acgcgcgtca acggaagacc
      421 tggcctcgtc ggctacttcg gggacggcag tccccagagc gtggttagca tcgaagtggc
      481 cgaagggagc atcagggcca tcaggctcgt cgtcaacccc gagaagctcg gtaacgtgcc
      541 gccgctcgaa agagaggagg actcgagatg aacccgccgc gagtcctcgt cacgggaggg
      601 acaggggtgc tcggccggcg cgtgcgttcc gcgaaggcgc gctggtgtgc ccggatcagg
      661 cttacggaaa gttcggctgg gaagacttct tgtgcccgcg gatagcgaca gataaggtgg
      721 taaccgggcg atgaatgatg cgacgcagac cctcgaacca ttcgtcgggc agtgggcggt
      781 cttgctgacc acgtacaagc gcgacggtac gccggtcggc acagcagtca acatcgcagt
      841 gcagggggat cgtgcctact tccgtacctg ggacacggcg tggaagctga ggcgcatccg
      901 caataaccca gaagtcgagt tcgccgcttc cacggcccgc ggtaggccta cggcacccgc
      961 gataggggcc cgcgccaggg tgctggaagg tgaaggatct gcccgcgcag gaaggctgct
     1021 cgcccgcaag tatcctgccc tgcacggcct ccttgtccca ctggtccatc gcctcagggg
     1081 caacaagacg atgcacgtcg agctaacgcc cgcggaagct ccgtcgcaga ttacctcggc
     1141 gccatccgga tcgcaccgtc cagcctgatg acctcgccgt tgagcatctc gttctcgaag
     1201 atgtgcttga cgagatccgc atactcctcg ggcctgccaa gccgtggagg ggcagaacct
     1261 gtttgcccag aggggcacgg gcttcttctg gcaggccggc catcatcggg gtgtcgaaga
     1321 tgccaggtgc gatggtcatg acgcggatac cactgccggc tacgagaaac atgttctcct
     1381 ctatgcgcac cttctacctc cccggttgag gatatgccgt tctcaaaacc gcgtcccagg
     1441 ccctgtctgg caggagacgc ctgagccgca taaacaggtg cgccgaaggt gtgacggcgt
     1501 agcggctcct ggggttgcgg tcggagaccg catgctctat agcctcggcg accagcccac
     1561 gtccacaagg cgctcggcgg tggcccatcc gatgcctgag gaacagccgg tgatcagcac
     1621 cgctttcgag acgtcatccc tctacccttc tactacggtc aggctgatcc tgctccgtcg
     1681 ctccgcgtcg ttgaagatgg tctggtgggc tacttcggtg cgggaggacg ctagtgtgct
     1741 ctcgcccgta ttgaggttgc gctcccagcg gggatgggag ctggatgtga tctccacccg
     1801 cagcctgtgc cccgggagga aggtgatgcc cgtagcccaa aggtctatga cgtactcgta
     1861 gacctcacct ggctcgatgg gggatggcgc cacaggcgtg accactccag gcgcggggta
     1921 gctctccctg gcgctcgccc gcaggatgcc atcagcgacg cagatagacc gcccgtcagg
     1981 gtagacgtcg acgagccgcg ccacgaagtc cgtgtcgggg gctgaggagg ccgcgaagag
     2041 cgtgacgtag accgagccca ggaccgtcag aggcctctcc agggtcgggc tcgtgtatac
     2101 gagcacgtcg ggcctgctct ctatagaccg ctgatccctc ggccccgagc cgtgaatggc
     2161 ggccataagc acgggaccac ccacggtcgg gacgggatcc tcggggtcgt agtcgaagcg
     2221 cgcaggctcg tccctcgctc cacccggttc gcggcacagc gagccatcgg agcgcaggtg
     2281 ccaatcctct gtacgcgccc ttggaggctg ccactcctcg tagccgcgcc aacggttctc
     2341 tcccatgacg aacaccttta ccgggggcgt accttcgagg gcgctctcgt ctccctttat
     2401 ggtggcgtcg aaccagcgga gatgggcgtc tgtcaggtcg cccctgtagt ccaggaacat
     2461 gcccgagctc ccgatgccga agtctagctc cccgaaggtg ctgccgaaat ccccgtgcgt
     2521 ccacgggccg accagaaggt gcggcggacg tagccctgcc tcgcgagagc attccttcat
     2581 cgcctcgtac tggcggagcg tctccccgat aaagcagtca taccaaccgc cgatgtggaa
     2641 ggttggggcg tctacgtttt cgtacttgcc ctcgatgttg aggtactccc agtcttcgtg
     2701 gtgtacgccg cgtccgaagc ctccctccac aaaagggacc agctcgtccg ggttcggcag
     2761 ctctgtgagt ggcagcacgt cgtagccgcc gcctgccagg agggtgtcta tggtgtggat
     2821 tacctcaggg agcttctccc cgatgagctc tcggtcgccg gcatacttgc ggaagaggag
     2881 gtcgagggtg agtgccgtct gcgcccagaa ttgcatcagg ccgagctcct gtacgccgcc
     2941 gcgcatctgg acgccgttaa ggtggttgcc ccaggtgagg cctgggacca tgctcttcag
     3001 cgagggcggc cgcatgacgg ccgcgtgcca ctgggtcttc ccaaagtacg agaggccgta
     3061 catgccgacc gacccgttgg aatcggggag cttcgcggcc cactcgacgg tatcgtagcc
     3121 atcctcgtac tccttgacga aagggacgaa ctttccttcc gaccggtaac gtccgcgcac
     3181 gtcctgcacg acgacgatgt agccggcctc cgcggccgtt acagggtcaa ggaccgacgc
     3241 gccagatggg aggtccttgc cgtatgggag gcgtgtcagc aggacgggat acccaccgcc
     3301 gccggcaggc ctgtagacgt tggacatcag ggtcgcgccg tcgcgcattt cggcaggcac
     3361 gttcttctgg agcaggatct ctaccatcag gcgcgccttc ggagcacgag aacagccgcg
     3421 atcgagagcg ccagtgcgac cggtacggcg gtggcaagcg tcctcaccgc tttcgtcccg
     3481 cgacgcaaca gggtcgcgtc gttgaggtgc gggaaagtct cttctggctc gggcacgccg
     3541 aggaactcac acaacggcac ccaaccctcc gtcacctcgt agaccagcag gcgttcggaa
     3601 gggacgcggc gtttgacctc ttcgttgtgc cgctcgaaga cctcgatggc gtacgccttg
     3661 tcctcgaagc gcccgtcgaa ggtgccctgc cagatcatgt cgttcacgat gctgcttctg
     3721 cccccggcaa acccgccgaa gacgaggagg ctgatcaggc cgaagatcag acgcgctatg
     3781 ggggagcttt cgagaagcat gctcaactcg tagatggtgc tgcgggtgct ctcgtaccac
     3841 ggttccggat cgcgcacgct aaggagcacc ttcgcctctg ggtgccgctc cataagctcc
     3901 tcgtagaagg tgcaggccgg ccaatccacg gtggcctcgt agcccccgag aactccttcc
     3961 caatccaccg gctctccgcg ccaggccgag ctccaaaagt ctgcgtgccc ggggtgggcg
     4021 aagacttccg tcatgtggta acaagggccg aagccgagca cctcgagcgc cgccttcagt
     4081 gaccaggttc ccgtccggcc gaagccggcc cctatgacct tcacgctagc tcttcgggga
     4141 cgtatagcct tcgaactgct cgcgcagctt caacttctgg aacttgcccg ttgcggtcat
     4201 cgggatggcc tccacgaact cgtaggcgtc gggcagccag aacctggcga agtccacttc
     4261 caggtgggtg tgcagctcat cctccgtcgc gctctcgcct tccctgagga cga
//
LOCUS       DATMJA010000286         5411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_689216, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000286 DATMJA010000000
VERSION     DATMJA010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689216"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="meaB"
                     /locus_tag="VNT12_06780"
     CDS             complement(<1..825)
                     /gene="meaB"
                     /locus_tag="VNT12_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWI45859.1"
                     /translation="MAKKGLVERLLAGDWRALARVISKIEREDEETAGIIAEIYPETD
                     DALTVGFTGPPGVGKSSIIAKLIELYRQEDRRVGVVSVDPSSPFSKGAILGDRIRLTD
                     HFLDAGVFIRSMGSRGHLGGLAGGSRLAAIAMAAGGYDVILYETVGVGQGEVEVASAA
                     DTVVLCLQPGTGDSVQALKAGVMEIADIFCINKADNPQAKNAASEVRSILEIGHELDP
                     DPWFPPIVMTRGDTGEGLQELKDTIAKHHRFLEQDGRLGKRRRAALKDFVISWATNR"
     gene            complement(841..1350)
                     /locus_tag="VNT12_06785"
     CDS             complement(841..1350)
                     /locus_tag="VNT12_06785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWI45860.1"
                     /translation="MGRRAEFLDIQKPLKKKYRNEPGSSRITLEAKASQQNTAISCSV
                     DVGRAVYEAEAHAGTGGTGEVACSGDLLLGALAACAQVTCQMVAEAMGIETERIEVTV
                     TGEMDLAGTLGVFEDVPVGFETIRTSFDIVAPEATEEQLVNLRQKTEQYCVVFQTLAE
                     TPDLQTEWA"
     gene            complement(1598..1822)
                     /locus_tag="VNT12_06790"
     CDS             complement(1598..1822)
                     /locus_tag="VNT12_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45861.1"
                     /translation="MKSACRYAPGTAAPNVNGEVHAELDTFALAVYGVPWRGKAGKTD
                     RDVLLALVGRARRREERQRQRHAADAIGFT"
     gene            complement(2018..2467)
                     /locus_tag="VNT12_06795"
     CDS             complement(2018..2467)
                     /locus_tag="VNT12_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI45862.1"
                     /translation="MEQSMDALSQIIKSERAIRGWSQDELAKRSGVSRKTISDIENGS
                     LKRAPQLRTVHKLLWVLPTEAPPPGAKSPEEAWEEIKGFVDLLDKTFEGGTTDLLATC
                     SVLGLRIVRVLEENAPTALFLHQEQTEYAIRRAISRDYLEPKDELAE"
     gene            complement(3059..4360)
                     /gene="odhB"
                     /locus_tag="VNT12_06800"
     CDS             complement(3059..4360)
                     /gene="odhB"
                     /locus_tag="VNT12_06800"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HWI45863.1"
                     /translation="MAAEIHVPELGESVADATVGRWLKQEGEAVKSGEALVELETDKI
                     NFEVEAEQDGVLQSISKDEGETVNVGEVIGTIGEGDGAAPAEVQEEQPQVEEEREEQP
                     QAEAEQQEPEDSGDETSNGHRELEDGAGRASPSVRKLAQEYEVDLAEVTGSGSGGRIT
                     RDDVERLIREQSSPTAPEKTEEPERDGEAQPAPAASADGRAALEERVRVSRRRQTIAQ
                     RLVESQQTAAMLTTFNEVDMSAVMELRKRRKESFQERTGARLGFMSFFTKAAIGALKA
                     FPKVNAELQGSELLLKKYYDIGVAVSTDEGLVVPVIRDADHKNFAEIEQGIADLATKA
                     REGKLTLEDLRGGTFTITNGGIFGSLVSTPILTMPQVAILGMHKIQERPVVVEGEVVV
                     RPMMYLALSYDHRVIDGAEAVSFLVRIKELVEDPESLLLEG"
     gene            complement(4384..>5411)
                     /locus_tag="VNT12_06805"
     CDS             complement(4384..>5411)
                     /locus_tag="VNT12_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HWI45864.1"
                     /translation="DTERGTFSQRHAVLYNAEADERYVPLQNLPQSGASFSIYNSPLS
                     EIAVLGFEYGYSVNSDDALVLWEAQYGDFANVGQPMIDQFIVSGQAKWGQTSGLVLLL
                     PHGYEGQGPEHSSARLERYLQLAAHENVRIANLTTAAQYFHLLRAQAALKDNKRPLVI
                     MTPKSLLRHPMAASRLEDLSEGSFQPVLDDEGVRERAESVERLILCSGKIYTELAGSE
                     YRGEAVDTAIVRIELLYPFPEDRVREVIDGYPNLREILWVQEEPKNMGAWTFIEPLLR
                     EISRGELPVIYVGKPPRPSPAQGSTKFHKQEHADIVRSAFESVRQDFDDVQVVEETQV
                     RASAPSE"
BASE COUNT          922 a         1730 c         1613 g         1146 t
ORIGIN      
        1 gcggttcgtg gcccatgaga tgacgaagtc tttcagggcg gcccgccgcc tcttaccgag
       61 cctgccgtcc tgttcgagaa accgatggtg cttcgctatg gtgtctttga gttcctggag
      121 gccttcgccc gtatcgccgc gggtcatcac gatgggcgga aaccacgggt cggggtcgag
      181 ctcgtgtccg atctccagta tggaacgcac ctctgaggcg gcgttctttg cctgagggtt
      241 atccgccttg ttgatgcaga agatgtccgc tatctccatg accccggcct tgagcgcctg
      301 caccgagtca cccgtacccg gctgcaggca aagcaccacc gtgtctgcag ccgaggcgac
      361 ctcgacctct ccttgaccaa ccccgacggt ctcgtagagg attacgtcgt aacctccagc
      421 cgccatcgcg atggcggcga gtcgcgagcc tccggcgagc ccgcccaagt gcccgcgact
      481 acccatcgag cggatgaaca ctccggcgtc gaggaagtgg tccgtaagcc ttatgcggtc
      541 accgagaatg gcacccttcg aaaaagggct cgaaggatcg acagagacca ccccgacccg
      601 tctgtcttcc tgcctgtaga gctctattag cttcgcgatg atgctgctct ttccgacgcc
      661 gggcggtccc gtaaagccta cggtcagagc atcgtcagtc tccgggtaga tctctgcaat
      721 tatcccggcc gtctcttcat cctcgcgctc tatcttcgag atcacccgcg ccaacgcccg
      781 ccagtcgccc gcgagcaacc gctctacgag ccctttctta gccaagctgg gtccgatctt
      841 ctacgcccac tccgtctgga ggtccggtgt ctctgccagc gtctggaaga cgacgcagta
      901 ctgctctgtc ttctggcgca ggttcaccaa ctgttcttcg gtagcctcag gcgccacgat
      961 gtcgaagctg gtgcggatag tctcgaagcc gacggggacg tcctcgaaga cgcctagcgt
     1021 tccggcgaga tccatctccc ccgtcacggt gacctcgatg cgctcggtct ctatccccat
     1081 ggcctcggcg accatctggc aggttacctg ggcacaggct gccaacgctc ccagcagaag
     1141 gtctccggaa caagcgacct ctcctgtacc gcctgtgccc gcgtgggcct cggcctcgta
     1201 gacggcgcgg cctacatcca cggagcagga gatcgcggtg ttctgctggc tcgctttggc
     1261 ttcgagcgtt atccgggaag atcccggctc attccggtac tttttcttaa gcggcttctg
     1321 tatgtccaaa aactccgccc gtctgcccat ctgccctaac ccatcccatc gctttaatgc
     1381 ccgactaaca gatgatactc ctgaagtatc gctaatgcgc gcccggacgc cctaacacgc
     1441 cctgtaggtg cctacgtagc cgttgtaggc gttcggtgtc ggcgcggata ggtcacccat
     1501 gacccgcacg cccggaagct gagagcgggc cgcatggata ccgctggcgt gagcgtggcg
     1561 gcttctcatc catctgcccg ctcgctttct attcggctta agtgaaacca atagcgtccg
     1621 ccgcgtggcg ctgccgctgg cgctcttcgc ggcggcgtgc tcgcccgacg agagcaagca
     1681 acacatcgcg gtcggtcttg ccagccttcc cgcgccacgg gacgccgtag acggccagcg
     1741 cgaaggtgtc tagctcagcg tgaacttcac cgttaacgtt cggcgctgcg gtacctggag
     1801 cgtaacggca agcgctcttc acgatgccgt ctatctccgt gtgctccagc ggtggtgagc
     1861 acctattggc atcttgccta accggtcact cgacgcgcta gccctggtct gttgttagcg
     1921 tatccccctt cgtctatcgc ttacgtgatt ttaagccccg gtctaccccg ccagcactat
     1981 gtcattcaca gatatatcgt ttgccgatat aatatcccta ctcagcgagt tcgtctttcg
     2041 gctcaaggta gtcgcgagag atagccctgc ggatcgcgta ctctgtctgc tcctgatgga
     2101 gaaacagggc ggtgggtgcg ttctcttcca gcacgcgaac gatgcgcaag cccaggacgc
     2161 tgcacgtcgc taacagatcg gtcgtgccgc cttcgaacgt cttatccagc aggtccacga
     2221 atcccttgat ctcttcccac gcttcttcgg gcgacttcgc gccgggtggc ggcgcttcgg
     2281 ttggtaatac ccagagcagc ttgtgaacag tgcgtagctg cggcgcacgc ttcagagaac
     2341 cgttctcgat gtcggaaatc gtcttgcgac tcacgccgga gcgcttggca agctcgtcct
     2401 gcgaccagcc acgtatggcg cgctcgctct ttatgatctg cgaaagagcg tccatcgatt
     2461 gttccatgcc catcccccta gagcggtgtc tcgttcaccc gctcattgta ggcttcagac
     2521 atgattataa caagtattga gtagaaaact tatatacatc tatctagttc tatgcactta
     2581 cggtataatg tggggagtag aaaccggccc ggcgggtgca gcaacacccc tggagcgcgg
     2641 cccacaggaa ggataagaaa ctgtggacaa ccagagtcta gcccgatccg ggcgcgaaac
     2701 caacgcagag agcactcacg gctgtcccgc ctgctatgaa ggacacagtg gacgcggatg
     2761 tagagctgga aataatgggt tacgtgccgg acgtggatga tttggatgaa agaaagccgc
     2821 gcaaagcggc ctctgcaccg accggagatc caagatcttc ctccaaagat ggctgccctt
     2881 ccgcagtgta ttgtagccgt ttggttgagg agaaggattg cacggcgcga gacacgttcc
     2941 tgatcctctg gcaacgatgg atgcgcggct ggcgcagact ccgggcatcc gcgctgtcgt
     3001 cgaggtctac gggtagacta aggggctccc aaatggaagc ccctgaccac gtccttcact
     3061 agccttctag cagtagcgac tcggggtctt cgaccagttc tttgatgcgg accaggaagc
     3121 tgaccgcctc ggcgccgtct atgacccggt ggtcgtagga gagcgcgagg tacatcatag
     3181 gacggaccac gacctcacct tcgacgacga cggggcgctc ctggatcttg tgcatcccca
     3241 gtatcgcgac ctggggcatg gtgaggatgg gcgtagagac cagcgagccg aagatgccac
     3301 cgttggttat ggtgaacgtt ccgccacgca ggtcttcgag ggtcagcttg ccctcgcggg
     3361 cctttgtggc caggtctgct atgccctgtt cgatctccgc aaagttcttg tgatcggcgt
     3421 cgcggatgac ggggacgacg agtccctcgt ccgtggagac ggccacgcct atgtcgtagt
     3481 acttcttgag aaggagctcg ctcccctgca gctcggcgtt gaccttgggg aaagccttca
     3541 gcgccccgat ggcagccttg gtgaagaagc tcatgaagcc gagccgcgcg cccgtccgct
     3601 cctggaagga ctccttgcgg cgcttcctta gctccatgac cgcgctcatg tcgacctcgt
     3661 tgaaggtggt tagcatcgcc gccgtctgct gcgactcgac caacctctgg gcgatggtct
     3721 ggcgcctgcg ggagacgcgc acccgctcct ccaacgccgc cctgccatca gcagaagcgg
     3781 ccggcgctgg ctgcgcttcg ccgtcccgct cgggttcttc ggtcttctcg ggggccgtgg
     3841 ggctggattg ttcccggatc aatcgctcca catcgtcgcg ggtgatgcgt ccgccggaac
     3901 cgctgccagt gacctcggcg aggtcgactt cgtactcctg ggccaacttt ctaacagaag
     3961 gggaggcgcg gccggcgccg tcctcgagct cgcggtgacc gttggacgtt tcgtcgccgg
     4021 aatcctcggg ctcctgctgt tcggcctcgg cttgcggctg ttcctcccgt tcttcctcga
     4081 cctgcggctg ctcttcttgt acttcggcgg gagcggctcc gtcgccttcg cctatggtgc
     4141 ctatgacctc gccgacgttg acggtctcgc cttcgtcttt ggagatggac tggaggacgc
     4201 cgtcctgctc ggcctcgact tcgaagttga tcttatctgt ttcgagctcg acgagcgcct
     4261 cgccggactt tacggcttca ccctcttgct tgagccagcg tccgaccgtc gcgtcggcga
     4321 ccgactctcc aagctcggga acgtgtatct cagcagccac tgctcactct tcctctctaa
     4381 ctctcactcg ctcggggcgg aggcgcggac ctgcgtctcc tccacgacct gtacgtcatc
     4441 gaagtcctgc ctgacgctct cgaaggccga cctcacgatg tcggcgtgct cttgcttgtg
     4501 gaacttggtc gatccctggg cggggcttgg gcgcgggggc tttccaacat agattacggg
     4561 taactcgccg cgtgaaatct cacgcagcag cggctcgatg aacgtccagg cgcccatgtt
     4621 cttcggctcc tcctggaccc acaggatctc ccgcaggttc gggtaaccgt cgatgacttc
     4681 ccttacccga tcttcgggga aggggtagag gagttcgata cggacgatgg ccgtgtccac
     4741 cgcctcgccc ctgtactcgc tgcccgccag ctccgtgtag atcttgcccg agcacaggat
     4801 gagacgctcg acagactcgg ccctctcgcg caccccttca tcgtcgagta cgggctggaa
     4861 ggagccttca gagaggtcct ccagcctgga ggcggccatg gggtgcctga ggaggctctt
     4921 gggcgtcatg atgaccaggg gacgcttgtt gtccttgagc gcggcctggg cgcggagcag
     4981 gtgaaaatac tgggccgccg tggtgaggtt cgctatacgt acgttctcgt gcgcggcgag
     5041 ctggaggtaa cgctccaggc gcgcgctgga gtgctcgggt ccctggccct cgtagccatg
     5101 cggcaggagc agcacaagcc ccgaggtctg gccccacttc gcctggccac tcacgatgaa
     5161 ctggtcgatc atcggctgcc ctacgttggc gaagtccccg tactgcgcct cccagaggac
     5221 gagcgcgtca tccgagttga ccgagtagcc gtactcgaaa ccgagcacgg cgatctcgga
     5281 gagcgggctg ttgtagatcg agaacgaagc gcctgattgg ggcaggttct ggagcgggac
     5341 gtagcgctca tcggcctcgg cgttgtagag cacggcgtgg cgctgggaga aggtcccgcg
     5401 ctccgtgtcc t
//
LOCUS       DATMJA010000287         2950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_274850, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000287 DATMJA010000000
VERSION     DATMJA010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274850"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..384
                     /locus_tag="VNT12_06810"
     CDS             220..384
                     /locus_tag="VNT12_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45865.1"
                     /translation="MQSPYLPALLVRVAVTVADGGDAILDLTIIQARPEPEVPTVLLK
                     QLFVPDHQKP"
     gene            complement(525..1415)
                     /locus_tag="VNT12_06815"
     CDS             complement(525..1415)
                     /locus_tag="VNT12_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI45866.1"
                     /translation="MALTIGTGSFGDQEQGRRRRIRERDEMLRETGIAEVNGARIYYE
                     VAGEGDPLVLVHAGIADSRMWEDQLMAFADRYRVIRHDMRGFGMTAMVEGPFSHHEDL
                     RALLDSLDVERAHLVGCSMGGGAVLDFALEYPQRSGNLVLVGSAVGGFGPDIDPPRQW
                     DEILAAEEAGDLERVSELEVQVWVDGPGRSPEDVDASVRDLVREMNLIALRNEASGLG
                     DEWEPEPPAADRLPDVQAPTLLIVGDEDQPSVFAAADLLERELPNVRKFVMHGTAHVP
                     NMERPEEFNRIVLDFLRDYR"
     gene            1780..2016
                     /locus_tag="VNT12_06820"
     CDS             1780..2016
                     /locus_tag="VNT12_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI45867.1"
                     /translation="MLPGVEVVGVAYAGEEALALCRKEEPDVVLMDISMPKMDGISAT
                     REIKALLPRTAGYPSTCSRRKEAPGEPRSNAGER"
     gene            complement(2239..2685)
                     /locus_tag="VNT12_06825"
     CDS             complement(2239..2685)
                     /locus_tag="VNT12_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI45868.1"
                     /translation="MADRAKLIGFNHVAIEVGDIEEALAFYGHLFEIELRGRGPRMAF
                     IDAGDQFIALAEGENRSSDEHRHIGMVVNDRRVVRQALKEMGVEILPGRGLDFLDPWG
                     NRWQVVDYSEIQFTKAPEILKGMGQESLKKSEKALEELRRKGLAPE"
     gene            complement(2732..2917)
                     /locus_tag="VNT12_06830"
     CDS             complement(2732..2917)
                     /locus_tag="VNT12_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45869.1"
                     /translation="MAQELLLRNDEPVSFDGEFYRLNDALLISRSPRPGLQWLEMDEI
                     DALEAMAHSVLPQVSGA"
BASE COUNT          540 a          984 c          835 g          591 t
ORIGIN      
        1 cttcggaggt cctcggggtg ctacgcgagg accggaagcg gacgaaacgc gtcaccttcc
       61 tgctcgaccc ggaaggcagg atctccagga tctaccctga agtcaccccg gatacccacg
      121 ccgaggagat cctgcaagat gccgcctcga tttaatctct gaaggtggag acgcggtcat
      181 agcatggcaa gcgtcagcca accaggcaga tgatccggcg tgcagtcgcc ctatctccca
      241 gccctcctcg tcagggtagc cgtgacggtg gccgatggcg gcgacgcaat cctcgatctc
      301 accatcatcc aggcacgccc agaacccgag gtcccaaccg tgctcctcaa gcaactcttc
      361 gtccccgatc atcaaaagcc ctgacgcctc gttcgggtgc aggttgagtt ccatctggaa
      421 aagctcatct tcacaggaca ttcccacata ctacgcgccc accacccgaa aattcctgtg
      481 agccctgaca ctacgggttc gtagggtctt tctagatgcg gcccctagcg gtagtctctc
      541 aggaaatcca gcacgatccg gttgaactct tctggccttt ccatgttcgg cacgtgtgcc
      601 gtgccgtgca tgacgaactt gcgcacgttc ggaagttccc tctctagcag gtcggctgcg
      661 gcgaagacgc tcggctggtc ctcgtctccg actattagca acgtcggagc ctggacgtca
      721 ggtagccggt ccgctgcggg cggctctggt tcccactcat cccccagccc tgaggcttcg
      781 tttctgaggg cgataaggtt catctcacgg accagatccc tgacggaggc gtcaacgtct
      841 tcgggggatc tcccggggcc gtcgacccag acctggacct ccagctcgga gactcgctcc
      901 aggtccccgg cctcttcggc ggcgaggatc tcgtcccact gccttggagg gtcgatatca
      961 ggcccgaacc cgcccacggc agatcccacg agcacgaggt taccgctcct ctgcgggtac
     1021 tcgagtgcga agtcgagcac ggctccgccc cccatcgaac agcccacgag gtgcgcccgc
     1081 tcgacgtcca gagagtcgag tagagcgcgc aggtcctcgt ggtgggagaa cggtccctcc
     1141 accatcgcgg tcatcccgaa gccccgcatg tcgtggcgga tgaccctgta gcggtcggcg
     1201 aaagccatga gttggtcttc ccacatcctg ctgtccgcga tccccgcgtg tacgaggacc
     1261 agcgggtccc cttctcccgc aacctcgtag tagatccgcg cgccgttgac ctcggcaatg
     1321 cctgtctccc gaagcatctc atccctttcc ctgatacgtc tcctccgacc ctgttcctgg
     1381 tcgccgaacg atcccgtccc gatcgtaagg gccatcgcac cattcctttc tttcggccgc
     1441 agctcgacaa gagcagctgg caggcccgca tcttccggcc cttatggtac aggcgcacaa
     1501 tgggtcgggt gacccactca gaattaccca gcgtggtgtg gtccaaatat ccgaagataa
     1561 cgctgccagc gcagacgatc attcgaagta cagggcactc agtatctcta cggttcatcc
     1621 gtgacacgct tggtgaacag cggatacagt tagagtcgca ctggacttcg aagcggcaaa
     1681 ggggcgcgag gttttgagca ccgaaagcga agatgtcatc cgcgtggcgg ttgtagacga
     1741 ccagcggctg ttcaccaagg tatctccggc ctcgtgggca tgttgcccgg cgttgaggtg
     1801 gtgggcgtgg cctacgccgg cgaggaggcc ctcgcgcttt gccgcaagga agagccggac
     1861 gtcgtcctga tggacatctc catgccgaag atggacggca tcagcgccac gcgcgagatc
     1921 aaggctcttc tgccacggac cgcaggatat ccgtcaacct gcagtcggcg aaaggaggcc
     1981 cctggtgagc cgcgctcaaa cgcgggtgag cgctaaccag gggcctgagc atgacgagac
     2041 tggtcgtctc gtaggttcgc atcacaacgt cgttggaagg cctggctcct atgccgggcc
     2101 ttctccttgt ttcttcgtcc ctgtacatca tgcgcacggt ggtcatccac tcatcccgca
     2161 gccgggactt cgggtttcag cgttcggctc acactcttga tggcggccgg cacaaagact
     2221 caggcactcc cttctgactc actcgggggc gaggcctttc ctgcgcagtt cttcgagcgc
     2281 cttctctgac ttcttcaaac tctcttggcc catgcccttg aggatctctg gggctttggt
     2341 gaactggatc tccgagtagt caacgacctg ccagcggttg ccccaggggt cgaggaagtc
     2401 gaggcccctg ccaggaagga tctccactcc catctccttg agcgcctgcc gaaccaccct
     2461 cctgtcgttc accaccatcc cgatgtgcct gtgttcgtcg gacgaacggt tctcgccttc
     2521 ggcgagcgcg atgaactgat ctcctgcgtc tatgaacgcc atcctgggtc cgcgtccccg
     2581 cagctcgatc tcgaagaggt gtccgtagaa cgcgagcgcc tcctctatat ccccgacctc
     2641 gatggccaca tgattgaacc cgatgagctt cgcgcggtcg gccatcacgc cattatctac
     2701 tcgtcagggt cgtcccgcca ccccgcaaac gtcatgcgcc gctgacttgc ggcaggaccg
     2761 agtgcgccat ggcctccagg gcgtctattt cgtccatctc cagccactgc aaccctggcc
     2821 gcggcgagcg cgatattagc agagcgtcgt tcagccggta gaactcgccg tcgaaagata
     2881 caggctcatc gttgcggagc agcaactctt gcgccaacgg agcattcgtg cgagtagctc
     2941 tacttcacga
//
LOCUS       DATMJA010000288         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_749192, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000288 DATMJA010000000
VERSION     DATMJA010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749192"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VNT12_06835"
     CDS             complement(<1..538)
                     /locus_tag="VNT12_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI45870.1"
                     /translation="MPNPRATHETQKHPLLLNPILTQQGAYPLLRLDERRWELEEKGA
                     ELFDFGTGDPREPTDVRIRQALIDGIPEVSRYPSTAGKQGLREAFCGWMERRHGVTLD
                     PESEVLPATGSKEAIFHAPLAFLHHSHERRGVAYGTPGYPVYERGTLLAGGESLPVEL
                     KWENGFLLQPEAIDPERTR"
     gene            614..1864
                     /gene="lysA"
                     /locus_tag="VNT12_06840"
     CDS             614..1864
                     /gene="lysA"
                     /locus_tag="VNT12_06840"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWI45871.1"
                     /translation="MDDFHYEGGGLRCEDVPLSEIAASAGTPTYVYSHAALERAYTEL
                     DSAFAGLDHLVCYAVKANGNLAVLRTLAALGAGADIVSGGELYRVMRAGFDPKKVVFA
                     GVGKTEKELMSGLGERILLFNVESASELEHLDRICARFGKRARVALRVNPDVDAGTHE
                     HVATGRGGDKFGIPVDEALALAERVADYRCIDLIGVHQHIGSQITKLAPYTESVERSA
                     GLVDELKTRGFDIRYFNIGGGLGIPYKDEETLTPKELVDAIRPMLEAAGTKILCEMGR
                     YIAGAAGVLMTSVVCRKKSGEKSFIVADAGMNDLLRPSLYDAHHEVLSVDEDGSVANA
                     DLVGPVCESGDYLARDRELPDASEGDVLAIMDAGAYGFSMASNYNSRPRPAEVMVKGD
                     SWAVVREREHSADLVKGELVPAFL"
     gene            complement(1999..2484)
                     /locus_tag="VNT12_06845"
     CDS             complement(1999..2484)
                     /locus_tag="VNT12_06845"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HWI45872.1"
                     /translation="MVLVRVLIGAVWLNGGLEKLLNPSFPGQFAASLQAGGFVSQAPP
                     FFQDFMKGYVVPNAELFAQFMRAGELMLGIALILGLLTNLAAIGSVGLSTVILLSQGG
                     VGLGTGLGSPEFLTINVLVALISVVILFSPAAKAISLDSRLAERSPGLSPILINRRRE
                     D"
BASE COUNT          513 a          849 c          907 g          486 t
ORIGIN      
        1 tccgggtgcg ttcggggtct attgcctcgg gttgcagcag gaaaccgttc tcccatttca
       61 gttccacggg cagagactcg ccaccggcca gaagcgtgcc ccgttcgtag acggggtagc
      121 cgggcgtgcc gtaggcgacg ccgcgccgct cgtgcgagtg gtgcaggaag gccagaggag
      181 cgtggaagat ggcctccttg gagcccgtgg ctggcaggac ctcgctctca gggtcgagcg
      241 tgaccccgtg gcggcgttcc atccagccgc agaaggcctc gcgcagccct tgtttgcctg
      301 ccgtgctcgg gtacctgctg acctcaggga tgccgtctat cagggcctgg cggatcctca
      361 cgtccgtcgg ctcgcgcggg tcgccggtgc cgaagtcgaa gagctcagcc cccttctcct
      421 ccagctccca gcggcgttcg tcgaggcgca ggagcgggta ggcgccctgc tgcgtgagta
      481 tagggttgag gagtaagggg tgcttctgtg tctcgtgtgt cgcgcggggg ttcggcacgg
      541 agtatattct ccctacggct ccatctgcag agccagaaat atagcacgcc ccggctacat
      601 gaggaggtac gttttggacg actttcacta cgaggggggc ggactccgct gtgaggacgt
      661 gccgctctcc gagatagccg cgtcggcagg gaccccgacc tacgtctaca gccacgccgc
      721 cctcgagcgc gcgtacacgg agctcgatag tgcgtttgcg ggcctggacc atctggtctg
      781 ctacgcagtc aaagcgaacg ggaacctcgc cgtgttgcgg acgctcgccg ctttgggggc
      841 aggagcagat atcgtctccg gcggggagct ctacagggta atgcgggccg ggttcgaccc
      901 gaagaaggtg gttttcgccg gggtcgggaa gaccgagaag gagctcatgt cggggcttgg
      961 ggagcgcatc ttgctcttca acgtcgagtc ggcctccgag ctggagcacc tcgaccgcat
     1021 ctgcgcccgc ttcggcaaga gggcccgcgt ggcgctgcgc gtcaacccgg acgtcgacgc
     1081 cgggacccac gaacacgtcg caacagggag aggaggggac aagttcggta tccccgtgga
     1141 cgaggcgctg gctctggccg agagagtagc ggactaccgg tgcatcgacc tcatcggggt
     1201 ccaccagcac atagggtccc agatcaccaa gctcgcccct tacacagagt ccgtcgaacg
     1261 ctccgccggt ctcgtcgacg agctaaagac ccgcggtttc gatatccgct acttcaacat
     1321 cggcggcggc ctcggcattc cttacaagga cgaggagacg ctgaccccga aagagctcgt
     1381 ggacgccatc aggcccatgc tggaggcggc ggggacgaag atcctgtgcg agatgggccg
     1441 ctatatagca ggcgccgccg gagtactcat gaccagcgtc gtctgccgca agaagagcgg
     1501 cgagaagagc tttatcgtcg ccgacgccgg tatgaacgac ctcctgcgtc cgagcctcta
     1561 cgacgcacac cacgaggttc tctccgtaga cgaagacggc tccgtggcga acgccgacct
     1621 cgtcgggccc gtgtgcgagt caggtgacta cctcgcccgc gaccgcgagc tgccagacgc
     1681 ctccgagggc gacgtgctcg ccatcatgga cgccggcgct tacgggttct cgatggcctc
     1741 caactacaac tcccgcccca ggcccgcaga ggtcatggtc aagggagata gctgggccgt
     1801 cgtgcgcgaa cgcgagcaca gcgccgacct cgtgaaaggc gagctggttc cggcgttttt
     1861 gtaggcgtca gcctacaggt atcaggtgtc aggtttcggg ctccttatgt cctggtgcgc
     1921 catcccgtcg cggcagggag atcttgctga ggggagagaa ggttatgctg cgggctatag
     1981 agcgcgagga gtggaggact agtcctcccg gcgacggttg ataagaatag gtgagagtcc
     2041 ggggctacgc tccgcgaggc gggagtccaa ggagatagcc ttcgcggccg ggctgaacag
     2101 gatcaccacc gagataagcg ccacaagcac gtttatagtg aggaactcgg gggagccgag
     2161 gccagtcccg agccccacgc ctccctggga gagcaggatc acggtgctca acccgacact
     2221 gccgatcgct gcgaggttgg tgagcaaccc gaggataagg gcgatgccta gcatcagctc
     2281 tcccgcgcgc atgaactggg cgaagagctc ggcgttcggc accacatagc ccttcatgaa
     2341 gtcctggaag aaaggcggag cctgggagac gaagcctccc gcctggaggc tggctgcgaa
     2401 ctggccgggg aagctcgggt tcagcagttt ctccagacca ccgttcagcc agacggcccc
     2461 gatcaggacg cgcaccaaca ccatgccgtt cacgtgccgg actcctctca cagcggtgtt
     2521 tggaagggtc gacccttcca aacaccgcgc tggtcagcat ttcacccatt cagctttttg
     2581 tttgctgatg gctcgtcgag ctttaggcct ggaccttatg cacggcgctg tcacagactg
     2641 ccacgctctc ctatcctaca tacgcaccgg agagagttta ggatctagct gaaatgctga
     2701 aatgctaaac acaaccaatg ccggatacgc tgcgtatatc atggagaccc ccggt
//
LOCUS       DATMJA010000289         6755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_393746, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000289 DATMJA010000000
VERSION     DATMJA010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393746"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VNT12_06850"
     CDS             <1..354
                     /locus_tag="VNT12_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI45873.1"
                     /translation="GENQEELDALISGWTSQRNAEEVLEIMKEAGVPAGKLFTAEDML
                     SDEQYAARQNIVTVEDPEIGPFPMQNVVPRLSETPGEVRWTGPKLGQHNDQVFKEVLG
                     MAEEDLDGLRERGIV"
     gene            354..1235
                     /locus_tag="VNT12_06855"
     CDS             354..1235
                     /locus_tag="VNT12_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HWI45874.1"
                     /translation="MRIEIVDVGPRDGLQNEDRTLSPEVRAELCEKLAAAGVPRVEAA
                     SFVNPKRVPQMAGAEEVMSAIERSPGTTYAGLVLNEKGYERAIEAGVDEVRYAFPVTK
                     TFAKRNQNTTVADATQLAARLVERARLDGVRVSITLSAAFGDPFEGWVDPEHVLRIAA
                     SVAASGPDEIVLADTIGVGVPSQVRKLVEGVLVHGVTVACHFHDTRNTGIANAVAAVE
                     SGSTVLDASVAGTGGCPFAPRATGNIATEDLVYLLHNMGYDTGIDLDALIEVATWLAQ
                     QLNKELPGQVYKAGNFA"
     gene            1312..1518
                     /locus_tag="VNT12_06860"
     CDS             1312..1518
                     /locus_tag="VNT12_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45875.1"
                     /translation="MVGDDGSVDAGATESKREEIKASRGESSGFDHGDLPPLDEQRRM
                     IAETRRKFNAWLSGELGKARSNGR"
     gene            1750..3003
                     /locus_tag="VNT12_06865"
     CDS             1750..3003
                     /locus_tag="VNT12_06865"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWI45876.1"
                     /translation="MNDTRSTLTHLQGALSGERYPADELIGLDPVDERPLLARYDIEK
                     AARTLTEKSLVERRTGGLWRWAELLPVRDRSNVVYLGEGDTPLLPQPRLGEAFGVPRL
                     ATKAEGLNPTGSFKARGMAVAVSRAAELGVTSFIAPSAGNAAGALAAYGAAAGAKVTV
                     LTPVDATLVNQQEVLAMGARLVLVRGLISDCGKISTALAPKIGAFDVSTLKEPYRVEG
                     KKTMGFELAEQRGWRLPDVIVYPTGGGTGLVGMWKAFQELQTLGLIGPDRPRMVSVQS
                     EGCAPIVRAFEAGDRFAEPWQNAKTRASGIRVPSAVGDFLILDTLRESGGTAVAVGED
                     SISEMQKFAGRMGAGYVSPESAAALAAVPVLRAKGEIGHDDEVVVFDCGIGQKYPPPQ
                     NLPEPPVVDPADLDIDALTHSLRAQ"
     gene            complement(3094..4674)
                     /locus_tag="VNT12_06870"
     CDS             complement(3094..4674)
                     /locus_tag="VNT12_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HWI45877.1"
                     /translation="MAARPDYEKSGSNGEEEPVMHVRRNVYALRPGGARIRALKDGVR
                     AMKDTSAFPNNDPRSWAYQAAIHGSNRRPPDPQAQDSWNMCQHASFFFLSWHRMYIYF
                     FERILRAASGDPNLALPYWNYSDPAQRALPVPFREPSGAANPLFVSQRNAGINAGAEL
                     LGSDVSTTQMFLARNFLPREGSRLSFGGGRVPGPVHFSSFTGLLEAQPHNTVHVKVGG
                     WMGDPNTAAQDPIFWLHHANIDRLWERWLDRGEGRANPVNNGVWMDTEFAFFDEDGDQ
                     VEMSAREIIQTSSQLDYVYDDEPAGALGATGFSGAAARVGGAEVFVATSSVANGDAEE
                     PTLIGRTGGRGEGEMIELGAEPTRVAVELGAPAAAAAEMVEAQAAARAAGEQAPEGGE
                     EQRIVLGLDGVQYDESPGVTYEIYLNLPEGQQPDYTSDYYVGNLGFFGMEAGGHGEGG
                     HGHPANLSFDVTDNVRALRAKGEWHEREANVSFFARGLIPPPGEETSVAEAEARAASD
                     TAEPAGSPRVERVVFSVE"
     gene            complement(4893..5363)
                     /locus_tag="VNT12_06875"
     CDS             complement(4893..5363)
                     /locus_tag="VNT12_06875"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWI45878.1"
                     /translation="MSNDEPDNPGIKVPPPLIYLLPLLLGLMLDRKAHLPFLPSGVVR
                     IVGWPLIGCGAMIGVWWRKTMRDADAPVRTDRPVPRLTTAGPFDYSRNPAYLSLAMIY
                     AGIAALRNSLWAIIFLPLVLIVIQREAIGREERYLERTFGEEYLAYKARVRRWV"
     gene            5653..>6755
                     /locus_tag="VNT12_06880"
     CDS             5653..>6755
                     /locus_tag="VNT12_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWI45879.1"
                     /translation="MKHLFELIGLTVIVVSSAVFWMGADRVVVVPCDQDLDAMVNADD
                     PMIGTRFQLEGPCIYPVDAMVELNEGDEIAGPQATFIERPPAFDPEPTVTIVGSEGLS
                     NVIRAQGTVHLEWVNIVGGTGQYSSGGSPVAGTGSALAMGMASNTSTLYAVHITGTDA
                     AGITNAHGTFERIELDDTTQDPNFLGFTGSGLKAINEVEVKNSYIHDNQGNGLWCDVF
                     CHDSADHPNGFWVHENLVVNNGRAGIRFERVGDVADAGEALIENNEVHQNSLELSRGG
                     IDIRDAQNALVRNNVFGAKSIADVAYLPNASGVAIRATDSGRLDRPDLLNVDIVGNVL
                     NGEVIKGCESPDEIVYCFEAAAPPSLTDTTPPET"
BASE COUNT         1333 a         2121 c         2108 g         1193 t
ORIGIN      
        1 ggcgagaacc aggaagagct ggacgccttg atctctggct ggaccagcca acgaaacgcg
       61 gaggaggtac tcgagatcat gaaagaggca ggtgtcccgg cagggaagct cttcaccgcc
      121 gaagacatgc tctcggacga acagtacgcc gcccgccaga acatcgtcac cgtcgaggac
      181 ccggagatcg gaccattccc gatgcaaaac gtagtccccc gcctctccga aacaccagga
      241 gaggtgcgct ggaccggacc gaagttaggc cagcacaacg accaagtgtt caaagaggtg
      301 ctcgggatgg cagaggagga cctcgacggt ctgcgcgaac ggggcatcgt ctaatgcgca
      361 tcgagatagt cgacgttgga ccccgagacg gcctgcagaa cgaagacagg acgctctccc
      421 ccgaagtccg cgccgagctc tgcgagaaac tggcggccgc cggcgtccca cgcgtagagg
      481 ctgccagctt cgtcaacccg aaacgcgtcc cgcagatggc cggcgcagaa gaggtaatgt
      541 cggccataga gcgttccccg gggacgacct acgccggcct cgtcctcaac gagaagggct
      601 acgagcgggc catcgaggcc ggggtagacg aagtacgcta cgccttcccc gtgaccaaga
      661 ccttcgccaa gcgcaaccag aacaccaccg tggctgacgc gacgcagctc gccgcgcgac
      721 tcgtggaacg ggcccggcta gacggggtgc gcgtctccat cacgctctct gccgccttcg
      781 gagacccatt cgaaggatgg gtcgaccccg agcacgtact ccggatcgcc gccagcgtag
      841 cggcatcggg tccggacgag atcgtcttgg cagacaccat cggcgtggga gtgccaagcc
      901 aggtgcggaa gctcgtagag ggcgtcctgg tccacggcgt cacggtcgcc tgccacttcc
      961 acgacacgcg caataccgga atagccaacg ccgtagccgc cgttgagagt ggctccacag
     1021 tgctcgacgc ctccgtcgcc ggtacgggcg gctgcccctt cgcccctcgc gccacaggca
     1081 acatcgctac agaagacctg gtctacctcc tgcacaacat gggctacgac acaggaatag
     1141 acctcgacgc cctcatagaa gtagcaacct ggctcgcaca gcaactgaac aaagaacttc
     1201 ccggacaggt ctacaaagca ggaaacttcg catgaggacc ccgaggcaat agccaaagac
     1261 gttcgctgga gcctcatcag ccccgaagcc gcagagcgcg tctacggcgt cgtggtcggt
     1321 gacgacggct ccgtagacgc gggagcgaca gaatctaagc gagaggagat aaaggctagc
     1381 cgtggcgagt catcggggtt cgaccacggg gatctgccgc ccctcgatga gcagcggcgc
     1441 atgatcgccg agacccgccg caagttcaac gcgtggctct cgggggagct cggcaaagcg
     1501 cggagcaacg ggaggtaaca cccgtagtcc ccagcgaggc cgtaggcgac cgcaacccgg
     1561 acgcccacaa ggcgaacctc tacgacatag acgccaagta cggcgacgta gtccccgttc
     1621 gggagacgct cgattatttg gaggaggtcg ccgcggcaca cgctcgttga gcgccgatga
     1681 ttgaggatag ccttcccttc gacacgcgag tagaattgcc gcggttctga caggctttgg
     1741 ggggttgatt tgaacgacac ccgttctacg ttaacgcatc tgcaaggcgc gctttcgggg
     1801 gagaggtatc cggcggacga gttgatcggg ctcgacccag tggacgaacg ccccctgctc
     1861 gcccgctacg acatcgagaa ggcggctaga accttgacag agaaatcgct ggttgagcgc
     1921 cggacgggcg gcctgtggcg ctgggctgag ctgctgccgg tgagggaccg aagcaacgtc
     1981 gtgtatctgg gagagggcga tacgccactc ttgccgcaac cacggctcgg cgaggcgttc
     2041 ggcgtcccgc gcctcgccac gaaggcagag ggcctgaatc ccacgggctc cttcaaggcg
     2101 cgggggatgg ccgtcgcggt ctcacgggcg gcagagttgg gcgtgacctc gttcatcgcg
     2161 ccttcggcgg ggaacgccgc aggggcgctt gctgcctacg gcgctgccgc cggcgcgaag
     2221 gttacggtgc tgacgcccgt ggatgctacg ctcgtcaacc agcaggaagt gctcgcgatg
     2281 ggggcgcggc tcgtcctggt gcgaggtctt atctccgact gcggcaagat ctccaccgcc
     2341 ctcgccccga agatcggagc gttcgacgtg agcacgctca aggagccgta ccgggtcgag
     2401 ggcaagaaga ccatgggctt cgagctcgcc gaacagcgcg gctggcggct accggatgtg
     2461 atcgtctacc cgacaggcgg cggcacgggc ctcgtgggga tgtggaaagc tttccaagag
     2521 ctgcaaacac taggtctgat cggacccgac aggccaagga tggtgagcgt gcaatcagag
     2581 ggctgtgcgc cgatagtccg tgcattcgaa gcgggggaca ggttcgccga accctggcag
     2641 aatgcgaaga ccagagcctc aggtatcagg gtcccttccg ccgttggcga cttcctgatc
     2701 ctggacaccc tgagagaatc gggagggacg gccgtcgcgg tgggcgaaga ctccatctcc
     2761 gagatgcaga agttcgcagg acgcatggga gccggctacg tcagccccga gtccgccgcg
     2821 gccctcgccg ccgtccccgt actgcgcgcc aagggagaga tcgggcacga cgacgaggta
     2881 gtcgtcttcg actgcggcat cggacagaag tacccgccgc ctcagaacct tcccgaacct
     2941 cccgtcgtcg accccgccga cctggatata gacgccctga cacacagcct gcgcgctcag
     3001 tagcagcgaa ccaggaaagg tcgcctgtgg ctttcccatc tgttgtcgca agcgcttcgc
     3061 gcctccaacc agaatcgatc tacggattgc gtattactcc acgctaaaga ccacgcgctc
     3121 cacacgcggg ctgcctgcgg gttcggccgt atctgaggcc gctctcgcct ctgcctcggc
     3181 taccgaagtc tcctcgccgg gcggcgggat caaccctcgg gcgaagaagc tcacgttggc
     3241 ttctcgttcg tgccactccc cttttgccct gagagcgcgg acgttgtccg tcacgtcgaa
     3301 gctcaggttg gcgggatggc catgcccgcc ttctccatgt ccgcctgctt ccattccgaa
     3361 gaagccgagg ttgcccacgt agtagtcgct cgtgtagtca ggctgctgcc cttccggcag
     3421 gttgagatag atctcgtagg ttacgccggg gctctcgtcg tactggacac cgtcgaggcc
     3481 aagcacgatg cgctgctcct cacccccttc gggggcttgc tcgcctgcgg ccctcgccgc
     3541 cgcttgcgcc tccaccatct cggccgctgc ggcggccggt gccccaagct cgaccgcaac
     3601 cctcgtcggt tcggctccca gctctatcat ctccccttct cctcgtcctc cggtgcgtcc
     3661 tatgagcgtc ggttcttccg catcgccgtt ggccacggat gaggtggcca cgaagacttc
     3721 agccccccca accctggccg ctgctcccga aaatccggta gcgcccaggg cgccggctgg
     3781 ctcgtcgtcg tagacgtagt ctaactgaga ggaggtctgt atgatctccc gggccgacat
     3841 ctcgacctgg tcgccgtcct cgtcgaagaa ggcgaactcg gtatccatcc acaccccatt
     3901 gttcacgggg ttcgcacggc cctcgccgcg gtcgagccaa cgctcccaga ggcggtcgat
     3961 gttggcgtgg tgcagccaga agatggggtc ctgggccgcc gtgttcgggt cgcccatcca
     4021 acccccgact ttcacgtgaa cggtattgtg cggctgggct tcgaggaggc ctgtgaagct
     4081 cgagaagtgc accggcccag ggactcttcc tcccccgaag ctcaggcgcg agccttctct
     4141 cggcaggaaa ttgcgggcca ggaacatctg tgtggtggag acgtccgagc ccagaagctc
     4201 ggcaccagcg ttgatgccgg cattccgctg cgacacgaat agcgggttgg ccgcgccgga
     4261 gggctcgcgg aatggcacgg gcaacgctcg ctgggcggga tctgaatagt tccagtaagg
     4321 aagtgccagg ttggggtctc cggaagccgc ccgcagtatg cgttcgaaga agtaaatgta
     4381 catgcggtgc caggagagaa agaagaagct ggcgtgctgg cacatgttcc agctatcttg
     4441 ggcctgggga tcgggcggcc tccggttgga gccgtggatg gcggcctggt aggcccagct
     4501 tctcgggtcg ttgttgggga aagcactggt gtctttcatc gcccggaccc cgtccttcag
     4561 ggctctaatc ctcgccccgc ccggacgcag ggcgtaaacg ttgcggcgta cgtgcattac
     4621 cggttcctcc tctccattgg aacccgactt ctcgtagtcg ggccgcgcag ccatatcttc
     4681 caacgtcctc acaataaagc aggcgtcgat gaatccgcga tgaaatgtgg atgatttcgg
     4741 gcgaaaaagg agcacttcga tacagtttga acgctagagc tcgaaacgcc tggcgaagct
     4801 gactatcgtg gctcttcctc tattcaccac accctgtaga aaactcattg tgcgaacttc
     4861 gccttgaacg gggttctcgg aagttgcgcc cgctacaccc agcggcgcac cctcgcctta
     4921 taggccaggt actcctcccc gaaggtgcgt tccaggtaac gctcctcgcg ccctatcgcc
     4981 tcgcgctgga tcacgatcag caccaacggt agaaagatga tggcccacag cgagttccta
     5041 agggcggcta taccggcgta gatcatggcc agagaaagat aggcggggtt gcggctgtag
     5101 tcgaaaggac ctgccgtggt cagcctcgga actggcctgt cggtgcgcac aggcgcgtcg
     5161 gcgtcgcgca tggtcttgcg ccaccagacc ccgatcattg ccccgcaacc tatgagtggc
     5221 cagccgacga tacgcaccac gccgctcggc aagaagggga ggtgtgcctt tctgtcgagc
     5281 atcaacccca acagcagtgg tagcaggtag atcagaggtg ggggcacctt gatgcccggg
     5341 ttgtctggct cgtcgttgga catgtgtcct cctccttcac tcgctttccg cagcacacca
     5401 tcctatgtta ttcatccaat tgtacgggtc gcgtgcggaa accgacacgg taaaagaccc
     5461 acgggcatat gtggaacgac taactttctt cgaagtttcg cgcttggctc gcgagaagtc
     5521 tcaatagtaa tcgtgcaaag gagacgctct ttccctcgaa gttcatcgcg aagacatcgg
     5581 cattgtagta atgtgggttc ataaagcctg ggggaggcgt ctccgcgtag ccatccgagg
     5641 aaaggtggag gattgaagca cctcttcgaa ctgatcggcc tcactgtgat cgtggtctcg
     5701 tctgcggtgt tctggatggg tgccgatagg gtagtcgtcg taccgtgcga ccaagacctc
     5761 gatgccatgg tcaacgccga cgacccgatg ataggaaccc gatttcagct tgagggaccg
     5821 tgcatctacc ccgtcgatgc gatggtggaa ctcaacgaag gcgacgagat cgcaggcccg
     5881 caggccacct tcatagagcg cccccccgcc ttcgacccgg aacccacagt aacaatcgtg
     5941 ggtagtgagg gtttgagcaa cgtgatccgc gcccagggca cggttcatct ggagtgggtc
     6001 aacatcgtcg gtggcacggg tcagtattct tcgggtggct caccggtagc cggcacgggc
     6061 tccgctctcg ccatgggcat ggcgtccaat accagcactc tctacgctgt acacatcacg
     6121 ggcaccgatg cagcaggcat caccaacgcc cacggcacgt tcgagcgtat cgagttggac
     6181 gacaccaccc aggatcctaa cttcttaggc ttcacgggtt ctgggttgaa ggcgatcaac
     6241 gaggtcgagg taaaaaactc ctacatccac gacaaccagg gcaacggcct gtggtgcgac
     6301 gttttctgcc atgactccgc cgaccatccg aacggtttct gggtacacga gaacctcgtt
     6361 gtcaacaatg gtcgggctgg tatacgcttt gagcgggtgg gcgacgttgc cgacgcgggc
     6421 gaagcactca tcgagaacaa cgaggtacac caaaactctc tggagctctc caggggtggg
     6481 atcgacatcc gtgacgcgca gaacgcgctg gtgcgcaaca atgtgttcgg agcgaagagc
     6541 atcgccgacg ttgcctacct acccaacgcc agcggagtgg cgatacgcgc aacggacagc
     6601 gggcgattgg acaggccaga cctattgaac gtggacatcg tgggcaacgt cctgaacggc
     6661 gaggttataa agggctgcga gtcgcccgat gaaatcgtct actgcttcga ggctgctgcg
     6721 cctccttcac tcactgacac cacgccgcct gagac
//
LOCUS       DATMJA010000290         9661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_216188, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000290 DATMJA010000000
VERSION     DATMJA010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216188"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            554..1000
                     /locus_tag="VNT12_06885"
     CDS             554..1000
                     /locus_tag="VNT12_06885"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWI45880.1"
                     /translation="MSDDTIALSDKQLDQLARYFNDEVTFSRHIGAKVKEVEPGRSVI
                     TIDVKDIHLNGAGTLHGGVYASLIDTAMGLSVLALVGVRTATIEMNVHFLGAVSDGRI
                     TCQSQVVHRTRRTATAEAKVRDSEGNLVAMGTGAFRVFERKGNAIV"
     gene            complement(1262..2023)
                     /gene="fabG"
                     /locus_tag="VNT12_06890"
     CDS             complement(1262..2023)
                     /gene="fabG"
                     /locus_tag="VNT12_06890"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWI45881.1"
                     /translation="MGRLDGRVAMVTGASRGIGEATARRFAREGARVCLTDVKLEGAF
                     STARQLVNEGMDAFAARVDVTSRTEVENAVKEIVDRYGRLDILVNNAGVTRDDLLYKM
                     TDEDWRTVLNVHLGGAFLCTRAAQRHMVERNYGRIINISSTSALGNSGQTNYSAAKAG
                     IQGFTKTLAIELGRYGVTVNAVAPGFIETEMTRETAARLGVDFDDFVAQRVESIPVGR
                     AGWPEDVAAAILYFASEEAAFVSGQVLYVAGGPRD"
     gene            complement(2232..3290)
                     /locus_tag="VNT12_06895"
     CDS             complement(2232..3290)
                     /locus_tag="VNT12_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWI45882.1"
                     /translation="MSETIDVREGEDFDLRAVEQYLRSHVEKVPEGELEVSQFPSGAS
                     NLTYLLKVGDWEGVLRRPPLGPVPPKAHDMGRESGILARLNAVYPLAPKPYFFCEDES
                     VIGAPFYVMERRKGVVLDDTFPDGVEHDEELCRGISRTLVDKLVELHAVDAREAGLGD
                     LGKPDGFLQRQTEGWISRYEKAKTDEIEEVAPLTDWLARDIPESPPPAVIHNDYKLNN
                     LVLNPEDLTGVRAVLDWEMATVGDPLFDLAVSLSYWIEPGDPDELAAVMPTVTVTPGF
                     MTRKELIDRYESESGRNLSEMHWYVVFGYFKLAGILQQIYARYKNGQTTDERFATFGE
                     RVRTLILHAENLSRTGEV"
     gene            complement(3287..4093)
                     /locus_tag="VNT12_06900"
     CDS             complement(3287..4093)
                     /locus_tag="VNT12_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HWI45883.1"
                     /translation="MHETGVEDFLKRLRVLMTRQEVVPGRLSGATAVVFDVFMATTTL
                     LTILENGARNVYPVASLEEADQVKETLDTGSLLRGGEQDAARIEGYDHGPFPEEYAPE
                     VVRAKDVIFVTTNGTRAIADAAPADTVFLGTLRNARAVASHLEVSGADSIYLVCAGAG
                     GRFNLEDFLGAATVLSYMDVDGFEFNDGARLALDFAERHGQNIYETLKRGRAGRWFVD
                     NERMDAFDFVADVGASGLVPEVVGGKLRRANKRESPTPLGVSDAGQEGKS"
     gene            4234..4671
                     /locus_tag="VNT12_06905"
     CDS             4234..4671
                     /locus_tag="VNT12_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45884.1"
                     /translation="MSDLERRKREESKRGGGNGYLYLTFGIGFVVAAIMVIATAGVLA
                     AGVGFVIPAAIFLGLAVNDFQKQRHALPQPANKERELLSAIRTNGGSITPAEAAMETS
                     LTVREADGMLSELAGGGHLAVESRDGALFYSLPGRRSPELEGR"
     gene            complement(4712..5497)
                     /locus_tag="VNT12_06910"
     CDS             complement(4712..5497)
                     /locus_tag="VNT12_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWI45885.1"
                     /translation="MYETVLYEKDKGVANIALNRPEKLNAFDGTMHDELYRALDSVAE
                     DEEIRCVVLRGEGRGFSAGADLAQIVKEADGDPDLGKYLRDTYSRLVKRMVRIEKPII
                     AALHGPVYGAGVGLALACDLRVAAHSAKFSVAFIKIGLMPDAGVTFLLPRVIGLGRAM
                     EMSMLGDAVDAEEAYRIGLVNRVVADNSLTEEARNLANRLAVMPTVALGRIKHALYTS
                     FERDVETALEAEAEGQTFCGSTQDHKEGVTAFFEKREARFTGR"
     gene            complement(5563..6120)
                     /locus_tag="VNT12_06915"
     CDS             complement(5563..6120)
                     /locus_tag="VNT12_06915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI45886.1"
                     /translation="MITGMLDYGTRILGVSQNIMLAAVLISSFAQIFFLTGWSALSDR
                     LGRRPVYLAGAALLGLWAFPMFWLVNTKSAFLITLALFIGQMFLSMMYGPQAALFSEM
                     FSARVRYSGASIGYQGASVFAGGLAPIIMVSLLESTGTSLSVSLYVFAMAVVTFVSVY
                     LITETYGDEMTRDQARETEEVATTG"
     gene            complement(6120..6920)
                     /locus_tag="VNT12_06920"
     CDS             complement(6120..6920)
                     /locus_tag="VNT12_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI45887.1"
                     /translation="MTDSSAQAAAPRPSTVRKVALAAMAGSAIEWYDFFIYLTAAALV
                     FGPLFFPGASEVAAVLASFSTAAVGFVARPVGGILFGHFGDKLGRKPTLVIALLTMGT
                     ATTLVGLLPTYATIGVAAPIILFVLRFCQGLAVGGQWGGAVLLATEYAPEGKRGFYGS
                     FAQVGVPVGLVLGNSAFLILTAIMGESAFGAWGWRIPFLLSVVLIGVALYIQLRLEDT
                     PAFRQLEERQQEGEESEAGSPVLEVLREHPKQVLLAGARSSSSTAPTT"
     gene            complement(7125..9107)
                     /locus_tag="VNT12_06925"
     CDS             complement(7125..9107)
                     /locus_tag="VNT12_06925"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HWI45888.1"
                     /translation="MTETAEGTPLWEPSEQLKENARISNYMDWLKDEKDLSFSDYNEL
                     WEWSVGDLEGFWASIWEYCGIKASRPYERVLGKREMPGAEWFPGAELNYAEHVLRHAT
                     DRQDEPAILHQSELRSLGEVSWGELQEKTATLAAGLRELGVERGDRVAAYLPNVPEAV
                     IALLACASIGAVWSSCSPDFGAGSVVDRFKQIEPKILIAVDGYRYGGKDYNRIGVVAR
                     LQQEIPTLQKTVVLPYLSEDPDTGPLEDVVMWDELLAGHEGAEISFEQVPFDHPLWVL
                     YSSGTTGLPKAIVHSQGGILIEHLKKAVLHLDLEPDDRFFWFTTTGWMMWNLVVAGLL
                     TGSTILLYDGNPGHPDMNVLWEFAEKTGMTCFGTSASYITACMKADIEPGKDFDLASL
                     ESIGSTGSPLPPEGFEWAYEHIKEDLWLFSTSGGTDMCTAFVGGVPLLPVRAGELQAR
                     SLGARVEAFDEEGNPQIDEVGELVVTEPMPSMPIYFWNDEEGERYGESYFDVYPGVWR
                     HGDWIKVKENGACVIYGRSDSTINRGGIRMGTSEIYSAVDKVEEVADSLVVDVPREAG
                     SSFMPLFVVLQEGIELDDDLKNRIKGSIKENTSPRHVPNEIFAVPEIPKTLNGKKLEV
                     PVKKILSGTPPEEAASRESLSNPESLDRFAELAEEI"
     gene            complement(9135..>9661)
                     /locus_tag="VNT12_06930"
     CDS             complement(9135..>9661)
                     /locus_tag="VNT12_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45889.1"
                     /translation="VVNNAGLQHVAAVEEFPPERFSMILRIMLEAPFRIVQKALPGMY
                     ERGWGRVINISSIHGMVASPYKSAYISAKHGLEGFSKTVALEGGARGVTSNCICPAYV
                     RTPLVENQISDQASTHGISEDEVVEKIMLTEPAIKRLIEPEEVAEFAAFLCAPEASFI
                     NGASLVMDGGATAH"
BASE COUNT         1837 a         3059 c         2870 g         1895 t
ORIGIN      
        1 ccttcggtta ggaaagcgcg aaaccgtagc tcagtcgttc tttcaacttc ggggatttgt
       61 gacgagcaac acggctaccg aaatctcgcc ctctgagaag ggacccttga ggcagaaaat
      121 tcctcgaaag tgctagcatc aacacgatgg aagccactat ctgcgcctga ccattctgaa
      181 cgacgatgac aggcttcgct cggacacggg actccaacca cgtacaaact agataatgat
      241 ccacgtggat cattatctgc taagaagtta tctggcacct tgtctcagca agggtgcggc
      301 aacgagaccc tctttgaatc gatcgcggta aggtggaaga gcacccagca ccgcgctcga
      361 tcagcgagac accgaagtgt aaactgccga ctgtcaaagc cgccaatgta cctgcttctc
      421 aggaggcgag tggaagcgag aagccccaag ctcatattcc cactcgacct cacctggggc
      481 tccgcatcgg tagaaggttg gtgacctgaa tggccccagc ggcctgtggt gccgaaatag
      541 aagggagcgc ccggtgagcg acgatacgat agccctgagc gataagcagc tggaccagct
      601 cgcacgatat ttcaacgatg aggtcacctt ctcaagacac ataggagcca aggtcaagga
      661 ggtcgagccg ggacgctcgg tcattaccat agacgtcaag gacatccacc tcaacggggc
      721 gggcaccctc cacggcgggg tctacgcctc cctcatagat actgctatgg gcctatcggt
      781 gttggccctc gtcggcgtga ggactgcgac catagagatg aacgtgcact tcctcggggc
      841 ggtcagcgac gggcgcataa cttgccagag ccaagtcgtt caccgtaccc gccggacggc
      901 gaccgccgag gcgaaggtac gcgattcgga aggcaacctc gtcgccatgg ggactggcgc
      961 gttccgcgtg ttcgagagga agggcaacgc gatcgtttga gccacagcct cttatagccg
     1021 atctgcaagc cgccgagtgg ccaatcaatc ggccctcagc aattccaagc cctcagcgcc
     1081 cacactactc aggaaagtgc ggaatctcac ctcggtgggt tttgtaatct tcggctgata
     1141 tgaccttctc gtcaaagtac cgagcgagtc gggtcaactg tcctttgtcc agactcccta
     1201 cagtttcttc cataagctct ccttctggcc cgatcccaaa aacgcagcgt agagcaaccc
     1261 tttaatctct cggcccgccg gcgacgtaga gcacctggcc gctcacgaac gccgcctcct
     1321 cggaggcgaa gtacaggatg gcggcggcca cgtcctcggg ccatccagcg cggccgacgg
     1381 ggatgctctc tacgcgctgg gcgacgaagt cgtcaaagtc caccccgagt ctggccgcgg
     1441 tctcgcgggt catttccgtc tctataaagc ctggggcgac ggcgttcacg gtaaccccgt
     1501 agcgaccaag ctcgatggcg agggtcttgg tgaagccctg gatgcccgct ttcgccgcgg
     1561 agtaattggt ctggccgctg ttgcccagcg cgctcgtcga tgagatgttg atgatcctgc
     1621 cgtagttcct ctcgaccatg tgcctctggg cggccctggt gcacaagaac gcaccgccaa
     1681 ggtggacgtt caacacggtg cgccagtcct cgtctgtcat cttgtagagg aggtcgtccc
     1741 gagttacgcc ggcgttgttg acgaggatgt caagccgccc gtagcggtct acgatctcct
     1801 ttaccgcgtt ctcgacctcg gtgcggctcg tgacgtccac tctggccgcg aaggcgtcca
     1861 taccttcgtt cacgagctgg cgcgccgtgg agaacgcacc ctccaacttc acgtccgtaa
     1921 ggcatacccg cgccccctcg cgcgcgaagc gccgcgctgt agcctcacct ataccacgtg
     1981 acgctcccgt caccatcgca accctgccgt cgagacggcc caatgtgtgt cgctcctttc
     2041 agtcgctaag caattcagca cgcatcggcc tttcaggcct ccgctttcag catttcagct
     2101 ttttggtgtc tgttcgccgc ctgtgcccga ccacgaatca ctccctcctt aaccgcctct
     2161 tcaaacacgt catctcctcg ccgtccgctg ccaagctgac tagctgaaat gctgcctggc
     2221 gcagccaggc attagacttc gcctgttcgg gagaggtttt cggcgtgcag gatcaaggtt
     2281 cgtacccgct cgccgaaggt ggcgaagcgc tcgtctgtgg tctggccgtt cttgtagcgg
     2341 gcgtagatct gctgcagtat cccggccagc ttgaagtacc cgaagacgac gtaccagtgc
     2401 atctcggaga ggttgcgccc actctcactt tcgtagcggt cgataagctc tttgcgggtc
     2461 atgaatcctg gcgttaccgt gacggtcggc atgacggccg caagctcgtc gggatcgccg
     2521 ggttcgatcc agtacgagag cgagacggcg aggtcgaaga gcgggtctcc gacggtcgcc
     2581 atctcccagt cgaggaccgc acgtacaccc gtcaggtctt ccgggttcag taccaggtta
     2641 ttgagcttgt agtcgttgtg gatgacggcc gggggtgggc tctcggggat gtcgcgggcc
     2701 agccagtccg tgaggggggc gacctcttct atctcgtctg tcttcgcctt ttcgtagcgt
     2761 gagatccaac cttccgtctg ccgctgcagg aaaccatcgg gcttgccgag gtcccctagc
     2821 cctgcctcac gggcgtcgac ggcgtgcagt tcgacgagct tgtctacgag ggtgcgtgag
     2881 attccacggc acagctcctc gtcgtgctcc acgccatcgg ggaaggtgtc gtcgagaacg
     2941 accccctttc tgcgctccat tacgtagaag ggcgccccta ttactgactc gtcctcgcag
     3001 aagaagtacg gtttcggggc gagcgggtag acggcgttga gcctggccag gatgcccgac
     3061 tcgcggccca tgtcgtgggc cttcgggggg accggaccga gcggtgggcg gcgcagaacg
     3121 ccctcccagt ctccgacctt caagaggtac gtcagattgg acgcgcctga ggggaactgg
     3181 ctgacctcca gttccccttc tgggactttc tccacgtgtg accgcagata ttgctcgacc
     3241 gctctgagat cgaagtcttc gccttcgcgc acgtcaatgg tctcgctcaa ctttttcctt
     3301 cctggcctgc gtcggagaca ccgagagggg ttggactctc acgcttattc gcacgtcgga
     3361 gctttcctcc taccacctct ggcaccagcc cgctcgcgcc tacatcagcg acgaagtcga
     3421 aggcatccat cctttcgttg tcgacgaacc atcgtcccgc ccggccccgt ttgagtgtct
     3481 cgtagatgtt ctgcccgtgg cgctcggcga agtcgagcgc cagcctggcg ccgtcgttga
     3541 attcgaagcc gtccacgtcc atgtaggaga gaaccgtcgc cgctccgagg aagtcctcga
     3601 gattgaagcg gccacccgcg cccgcgcaaa cgaggtagat cgagtccgcc ccagagactt
     3661 cgaggtggct tgcgacggcc ctggcgttgc ggagggttcc gaggaaaact gtgtctgcgg
     3721 gggccgcgtc ggcgatggcg cgggtgccgt ttgtggtcac gaagatcacg tccttggcac
     3781 ggacgacctc gggcgcgtac tcttctggga agggaccgtg atcgtagccc tctatgcgcg
     3841 cagcatcctg ttcgccgccg cgtaagagcg agccggtgtc tagcgtctct ttgacctgat
     3901 ctgcctcctc caggctcgcc acggggtaga cgttccttgc gccattctcc aggatagtga
     3961 gcagggtcgt cgtcgccatg aacacgtcga agacgacggc cgtggcacca gagagtctcc
     4021 caggcaccac ttcctgccgc gtcatcagaa cccttagtct cttcaagaaa tcttcgaccc
     4081 ccgtctcgtg caagcccgca ccattctaca aaaacacgca ggtaggaacc tcatgggctg
     4141 cgcttgcacc gtggatggta gagactagaa tggtgtcgaa cgcgcgggtt ctctaacgcg
     4201 aatgactcgg agcttgagta aaccggaggc gtggtgagtg atctagagcg gcgcaaaagg
     4261 gaggaatcga agcgtggcgg gggcaacggg tacctgtacc tgacctttgg gatcggtttc
     4321 gtggtggctg cgataatggt cattgccacc gcaggagtac tcgcagcggg cgtcggcttt
     4381 gttataccgg cggcaatttt tctcgggctg gccgtaaacg actttcagaa acaacgacac
     4441 gccctgccac agcccgccaa caaggagcgg gagctccttt cagcgatcag gaccaacggc
     4501 ggcagcataa cgcccgcgga ggcggctatg gagacctcgc ttaccgtgcg cgaggcggac
     4561 gggatgctat cggagctcgc gggaggcggg catctcgcgg tggagagcag ggacggagca
     4621 ctgttctact ccctgcctgg cagacgcagc cctgagctcg aaggaaggta gtcggggcgt
     4681 ttcgcgtgta agggcgtttc gcgaaacgcc cctaccgtcc ggtgaacctc gcctcgcgct
     4741 tctcgaagaa cgctgttacg ccctccttgt ggtcctgcgt ggagccacag aacgtctggc
     4801 cctcggcctc ggcctccaga gccgtttcca cgtccctctc aaagctggtg tagagggcgt
     4861 gtttgatcct acctagggcc accgtcggca tcacggcgag gcgattggcc aggttccgcg
     4921 cttcttcggt gagggaattg tcggctacta ccctgtttac gagtcctatc ctgtaggctt
     4981 cttctgcgtc aacggcgtcg ccgagcatac tcatctccat agcccttccc aaaccgatga
     5041 cgcggggcag caagaatgtg acccccgcgt cgggcatgag ccctatcttg ataaaggcca
     5101 ccgagaactt cgcgctgtgt gctgcgacgc gcaggtcgca cgcgagcgcc aggccaactc
     5161 ccgccccgta gacaggcccg tgcagggcgg cgatgatggg cttctcgatc ctgaccatcc
     5221 gctttacgag ccggctgtac gtgtcgcgca ggtatttccc gaggtcagga tctccgtcag
     5281 cctccttgac gatctgcgcc aggtcggcgc ccgcggagaa accgcggcct tcgccgcgga
     5341 gcacgacgca gcggatctct tcgtcttccg cgacgctatc gagtgcacgg tacagttcat
     5401 cgtgcatcgt gccgtcgaag gcgttcagct tctccggacg gttcagggcg atgttggcca
     5461 cccctttatc cttttcgtag agcacggtct cgtacaactt ccctccttcg agaacggtgc
     5521 cccccgcatc cgaggggcac aaagacgaag cgggtggcga agctatcccg tcgtagccac
     5581 ctcctcggtc tcccgtgcct gatcccgggt catctcgtcc ccgtaggtct ccgtgatcag
     5641 gtagaccgac acgaaggtga cgacggccat cgcgaagacg tagagggaga ccgacagcga
     5701 cgtgcccgtg gattccagca gcgagaccat gatgatgggc gcgagacccc ccgcaaagac
     5761 agacgcgccc tggtagccga tcgaagcccc cgagtagcgc acccgcgcgc tgaacatctc
     5821 ggagaagagc gcggcctgcg gaccgtacat catgctcagg aacatctgcc cgataaagag
     5881 cgcaagggtg atcaagaacg cgctcttggt gttaacgagc cagaacatgg gaaaggccca
     5941 gagccccaac agcgccgcgc ccgcaaggta cacgggacgg cgtccgaggc ggtccgagag
     6001 tgcagaccat cctgtcaaga agaagatctg ggcgaaactg gagatcagaa cggccgccag
     6061 catgatgttc tgggacaccc caaggatcct cgtaccgtag tcgagcatgc cggtgatcat
     6121 cacgtagtag gcgccgttga cgacgaagaa cgcgcccccg cgagcaacac ctgcttggga
     6181 tgttcgcgga gcacctcgag cacgggcgag ccggcctcgg attcctcgcc ctcttgctgc
     6241 cgctcctcca gttgcctgaa cgctggggtg tcctccagac gcaactgtat gtacagagcc
     6301 acaccgatca ggacgacgct gagcaagaat ggtatgcgcc agccccacgc accaaacgca
     6361 ctctctccca tgatcgccgt gaggatgagg aaggcgctgt tcccgaggac gagcccgacg
     6421 ggaaccccaa cctgggcgaa gctcccgtag aagccccgct tgccctcggg ggcgtactcc
     6481 gtcgccagca agaccgcccc gccccactgg ccgccgacgg cgaggccctg acagaagcgc
     6541 aacacgaaga ggattatcgg ggcggcgacc ccgatggtgg cgtacgtcgg caagagaccg
     6601 accagcgtgg tcgccgtgcc catagtcagc aacgctatga ccagagtggg cttacgaccc
     6661 aacttgtccc caaagtggcc gaatagtatg cctcccaccg gacgcgcgac aaagccaaca
     6721 gcggccgtgc tgaacgaggc caagactgct gcaacctcgc tggcgccggg gaagaacagc
     6781 gggccgaaga cgagggccgc tgccgtcagg tagataaaga agtcgtacca ctcgatagcc
     6841 gaaccggcca tcgccgccag cgctaccttc cgcaccgtgg acggtctcgg cgcagccgcc
     6901 tgcgcgctag aatctgtcat acacgcctcc cttcaaacac cgtgttcctg acaacctcgc
     6961 gggctgaacc cgtaagtagc gctctcttga accaggttat acgatcccct ttccccaatg
     7021 catctctgtg agcagattag gccactaccg agttagagtc aatcgttact tcaaggatgc
     7081 aaagatgctg cccggcgcgc ggttcgcgcg ccgggtctat ggggttatat ctcctcggcc
     7141 agctccgcga aacggtcgag ggattcgggg ttgctaagag actccctgct tgcggcctcc
     7201 tcgggcgggg tgcctgagag gatcttcttg acgggcacct cgagcttctt gccgttgagg
     7261 gtcttgggta tctcggggac ggcgaagatc tcgttcggca cgtgcctcgg ggaagtgttc
     7321 tccttgatgc tcccctttat cctgttcttc aaatcgtcgt cgagctcgat accttcttgc
     7381 aggaccacga agagcggcat aaaggagctt cccgcttctc tggggacgtc gacgacgagg
     7441 ctgtcggcca cctcctcgac cttgtctacg gcggagtaga tctcgctcgt ccccatcctt
     7501 atgccgccac ggtttatggt ggaatcggag cgcccgtaga tcacgcaggc gccgttctcc
     7561 ttgaccttga tccaatcccc gtgacgccac acgccgggat agacgtcgaa gtagctctcc
     7621 ccgtaacgct ccccttcctc atcgttccag aagtagatcg gcatggaggg catgggctct
     7681 gtgacgacga gctccccgac ctcgtctatc tggggatttc cctcctcgtc gaaggcttcg
     7741 accctcgccc ccagggaacg ggcctgtagc tcccccgcgc ggaccggcag gaggggtacc
     7801 ccaccaacga acgcggtgca catgtcggtg cccccgctgg tcgagaagag ccagagatcc
     7861 tccttgatgt gctcgtaggc ccactcgaag ccctcgggag gcaacggcga acccgtagaa
     7921 ccgatcgact cgagtgaagc gaggtcgaaa tccttgcccg gctctatatc cgctttcatg
     7981 caggctgtga tgtagctcgc gctcgtacca aagcaggtca tgcctgtctt ttctgcgaac
     8041 tcccagagga cgttcatgtc agggtggcca gggtttccat cgtagagcag aatggttgat
     8101 ccggtcagca ggcctgcgac gaccaggttc cacatcatcc agcccgtcgt ggtgaaccag
     8161 aaaaagcgat cgtcaggttc gagatctaag tgcagcacgg ccttcttgag gtgctcgatg
     8221 agaatgccgc cctggctgtg aactatggcc ttcggaagac ccgtcgtgcc tgacgagtag
     8281 agcacccaga gcggatggtc gaaaggcacc tgctcgaaag agatctccgc gccttcgtgc
     8341 ccggccaaca gctcgtccca cataacgacg tcttcgagcg ggcccgtgtc aggatcttct
     8401 gaaagataag ggaggacgac ggtcttctgg agcgtcggta tctcctgttg cagcctcgcg
     8461 acgacaccta tcctgttata gtccttgccg ccgtaacggt agccgtcgac cgcgatgagg
     8521 atcttaggct ctatctgttt gaaacggtcc acgacgctgc cggcgccgaa gtcgggggag
     8581 cagctcgacc agaccgcgcc tatggaggca caggctagca gggcgatcac ggcttcaggg
     8641 acgttcggca ggtaggcggc gaccctgtca ccccgctcga cgcccaactc cctaaggccg
     8701 gctgcgagcg tggcggtctt ctcctgcagc tcgccccagc tcacctcacc cagtgacctc
     8761 aactcggact ggtgcaggat cgcgggctca tcctggcggt ccgtggcgtg cctcaagacg
     8821 tgttcggcgt aattcagctc ggccccgggg aaccactctg ccccaggcat ctcgcgcttt
     8881 ccgagcaccc tctcgtaagg cctcgaggct ttgatgccgc agtactccca gatcgaagcc
     8941 cagaaccctt cgaggtcgcc aacggaccac tcccagagct cgttgtagtc gctgaaagag
     9001 agatccttct cgtctttcag ccaatccatg tagttggaga tcctggcgtt ctccttgagt
     9061 tgttccgagg gctcccagag tggggtccct tccgcggttt cggtcacgag tctccctttc
     9121 cgagttcgaa ctctctaatg ggctgtcgcc ccgccgtcca tgacgagtga cgcgccgttt
     9181 atgaacgatg cctcaggcgc acagaggaag gcggcgaact ctgcgacttc ctctggttcg
     9241 atcaggcgtt ttatcgcggg ctcggtcagc atgatcttct cgaccacctc gtcctccgag
     9301 atcccgtgcg tactagcctg gtcagagatc tgattctcca cgagcggcgt ccttacgtag
     9361 gcaggacaga tgcagttgga ggtcacgcct ctggcccctc cctccagggc gacggtcttg
     9421 gagaagccct cgaggccatg cttggccgag atgtaggctg acttgtaagg cgaggcgacc
     9481 atgccgtgga tggaggagat gttgatgacg cgtccccagc ccctctcgta catccccggc
     9541 aaggccttct gcacgatcct gaaaggcgcc tcaagcatga ttctgagtat catggagaag
     9601 cgctcgggcg ggaactcctc tacggcagcg acgtgctgca gccccgcgtt gttgaccact
     9661 a
//
LOCUS       DATMJA010000291         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_97559, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000291 DATMJA010000000
VERSION     DATMJA010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97559"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2725
                     /gene="gltB"
                     /locus_tag="VNT12_06935"
     CDS             <1..>2725
                     /gene="gltB"
                     /locus_tag="VNT12_06935"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWI45890.1"
                     /translation="DLEKLSPVILPGQSDSASFDNALELLHLAGRSLPHAVSMMIPEA
                     WENDELMDPDRRAFYQYHSALMEPWDGPAAVAFTDGRIVGATLDRNGLRPARYSVTRD
                     GRVIMASEDGALRVPAEDVIERWRLQPGKMLVVDTERHELLHDEDVKGPLFGRQPYRR
                     WLEEGEIHLNELPEPDADPRPEPSSLLERQRAFGYTIEDQRILLAPMAQNGKEPDGSM
                     GTDTPLAVLSERPQLLFSYFKQHFAQVTNPPIDPLREELVMSLKMSLGPEQNLFDETK
                     KHCRRVLIDQPVLTAAELGKIRALGDERASSTTLSTLFPVSTGEEGMEEALGKLCEKA
                     ERAVKAGSAVLVLSDRGVNETQAPIPSLLATASVHHHLVRAGIRTATTLVVETAEARE
                     VHHFALLVGYGATAVNPYLAFETIEDLAGSHLMEGVGPEEAAKNYVKAVGKGLLKVIS
                     KMGISTLFSYCGAQIFEAVGLNQKLIDRCFTGTASRVGGVGMDALARETLERHRSAFS
                     AAWDNAEELDVGGEYQLRAQGQYHQWNQTSIPPLQRAVKTGNFETFKEFTRHFDEHSA
                     RFATLRGLFRFEESPVAIDEVESAREIVKRFTTGAMSLGSLSREAHETLAIAMNRIGG
                     KSNTGEGGEDPRRFTPDENGDSRRSAIKQVASGRFGVTTEYLVNADQLQIKMAQGSKP
                     GEGGQLPGYKISEYIAKIRYSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPRAKV
                     SVKLVAEVGVGTVAAGVAKAKADHITISGHDGGTGASPLSSIKHAGLPWELGIAEAQQ
                     VLVENGLRGRVILQTDGQLKTGRDVTVAALLGAEDFAFSTAPLVATGCIMMRVCHLNT
                     CPVGIATQDPELRKRFTGTPEHVINYFFFIAEEVREYMARMGFRTFEEMIGR"
BASE COUNT          534 a          860 c          879 g          452 t
ORIGIN      
        1 ggacctggag aagctctcgc cggtcatctt gccgggccag agcgactcgg cctccttcga
       61 taacgccctg gagcttttgc acctcgccgg gcgaagcctg ccgcatgcgg tctccatgat
      121 gatcccagag gcctgggaga acgacgagtt gatggatccc gataggcgcg ccttctacca
      181 gtaccacagc gccctcatgg agccttggga tggtcctgct gcggtcgcgt ttaccgatgg
      241 tcgtatcgtc ggcgccacgc tggaccggaa cgggttgcgt ccggcgcgtt actccgtcac
      301 cagggacggc agggtgatca tggcgtcgga ggatggtgcg ttgagggtgc ccgccgagga
      361 tgtgatcgaa cgctggaggc tccagccggg caagatgctc gtcgtcgaca ccgagaggca
      421 cgagctgctg cacgacgagg acgtcaaggg gccgctcttc gggcggcagc cgtacagacg
      481 ttggctcgag gagggtgaga tccacctcaa cgagctaccc gaacccgacg ccgacccgcg
      541 tcccgaaccg tcgtcgctcc tcgagcgcca gcgggccttt gggtacacta tcgaagacca
      601 gcgcatactc ctcgccccga tggcccagaa tggcaaggag cctgacggtt ccatgggcac
      661 ggacacgcct ctggccgtgc tctccgagag gccgcagctc ctgttttcgt acttcaagca
      721 gcacttcgcg caggtcacca atcctccgat agaccccttg cgcgaggagc tggtcatgtc
      781 gctcaagatg agcctcgggc ccgagcagaa cctcttcgac gagacgaaga agcactgcag
      841 gcgggtgctc atcgaccagc cggtcctcac ggcggcggaa ctggggaaga tccgggccct
      901 aggcgatgag cgggcgagtt ccacgacgct ctccaccctg ttccccgtct cgaccggcga
      961 ggaggggatg gaggaggctc ttggcaagct ttgcgagaag gccgagcgcg cagtcaaggc
     1021 agggtcagcg gttctggtcc tgagcgacag aggcgtgaat gagacgcagg ccccgatacc
     1081 tagcctcctc gccaccgcga gcgttcacca ccacctggtg cgggcaggca taaggacggc
     1141 cacgaccctc gtcgtcgaga ctgctgaggc gcgtgaggtc caccacttcg ccctccttgt
     1201 cggctacggc gcgaccgccg ttaaccctta cctcgccttc gagacgatag aagatctcgc
     1261 aggctcgcac ctcatggagg gtgttggtcc cgaggaggcg gcgaagaact acgtcaaggc
     1321 cgtggggaag ggcctgctca aagtcatctc caagatgggc atctccacgc tcttctccta
     1381 ctgcggggcc cagatcttcg aagccgtagg cctgaaccag aagctcatag acagatgctt
     1441 tacaggcacc gcctctcgcg ttggcggcgt cgggatggac gctttagcgc gcgagaccct
     1501 ggaacgccac aggagtgcct tctcggctgc ctgggacaat gcggaggagc tcgacgtcgg
     1561 gggtgagtac cagctcaggg cgcagggcca ataccaccag tggaaccaga cgagcatccc
     1621 tcccctccag cgcgccgtaa agacagggaa tttcgagacc ttcaaggagt tcacccgtca
     1681 cttcgacgag catagtgcgc gcttcgctac attgcgcggc ctcttcaggt tcgaggagag
     1741 ccccgtcgcg atagatgagg tcgagtctgc aagagagatc gtcaagcgct tcacgaccgg
     1801 ggcgatgtct ctcggctccc tctccaggga ggcgcacgag accctcgcca tcgcgatgaa
     1861 ccgcataggc ggcaaatcca acactggtga gggcggcgag gaccccaggc gcttcacgcc
     1921 ggacgagaac ggcgatagcc gtcgcagcgc cataaaacag gttgctagcg gccgcttcgg
     1981 cgtcacgacc gagtacctgg tcaacgcgga ccagttgcag atcaagatgg cgcaagggtc
     2041 caagccgggc gagggcggcc agttgcccgg atacaagatc agcgagtaca tagcgaagat
     2101 acggtactcc acgcctggcg tgggtctgat ctcaccgccg ccccaccacg atatctactc
     2161 gatagaggac cttgcgcagc tcatccacga cctgaagaat gccaacccaa gggccaaagt
     2221 tagcgtcaag ctcgtagccg aggtcggggt cgggactgtc gcggcaggcg tagccaaagc
     2281 gaaggccgac catataacca tctcgggtca cgacggcggg acgggggcct ccccgctctc
     2341 ctccataaag cacgcggggt tgccgtggga gctcggtatc gccgaggccc agcaggtgct
     2401 cgtcgagaac gggctgcggg ggcgtgtgat cttgcaaacc gacggccagc tcaagacggg
     2461 ccgcgacgtc accgtggcgg cgctcctcgg ggccgaggat ttcgctttct cgacggcgcc
     2521 gctcgtggcc acgggctgca tcatgatgcg cgtatgccac ctgaacacgt gcccggtggg
     2581 tatcgcgacc caggatcccg agctaagaaa gcgatttacc ggtacgcccg agcacgtcat
     2641 caactacttc ttcttcatcg ccgaagaggt gcgggagtac atggcgagga tgggcttcag
     2701 gaccttcgag gaaatgatcg gccgc
//
LOCUS       DATMJA010000292         6050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_630805, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000292 DATMJA010000000
VERSION     DATMJA010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630805"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VNT12_06940"
     CDS             complement(<1..334)
                     /locus_tag="VNT12_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HWI45891.1"
                     /translation="MNLTFYIVDVFAEEKYAGNQLAVVRGGAGLPDERLQKIALEINY
                     SETAFVLSEEETEGGYDVRIFTPGNEVPFAGHPTLGTAYVIQHEILACPVERITLKLK
                     AGEIPVTFG"
     gene            complement(343..1167)
                     /locus_tag="VNT12_06945"
     CDS             complement(343..1167)
                     /locus_tag="VNT12_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HWI45892.1"
                     /translation="MKIYISADMEGVTGVTHSQDVIPGRSQYERFRRLLTADVNAAIE
                     GAAQADVTEFLVNEAHDGMRNILLEDLDPRAEVIVGQRKPYSMMQGFEGADLVFFVGY
                     HARAGTEGVLSHTFDSPTVVTGVWLNGEPCSEARMNATLAGLKGIPVGLVTGDDLACA
                     EAESLYPGVQTARVKTVVDRYTARCLSPQVAKERIREAALRAVGVGDLSPYAPEPPYS
                     FTIEFATASSAASVLFFPGLERVDDRRISWTHEDYETAFKMFIGVMRLSRSDPDYG"
     gene            complement(1238..2680)
                     /locus_tag="VNT12_06950"
     CDS             complement(1238..2680)
                     /locus_tag="VNT12_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWI45893.1"
                     /translation="MSGAGVAGASLLAGCGSTSVLPDLPGKTPTADRNTNVVLIIMDS
                     LRKDHIGAYGNEKIQTPNLDALAEESLLFSRAYPESIPTICARRAIHTGLRTWPFEDW
                     RRYKGIDVGLQGWQPIPNDQITLAETMRSAGYETLFVTDNLQQYDASMNFQRGFEAFD
                     FIRGQTTDSYKPLYTCPPGKVEQALVNRAILGTGSRAYFQRYFANTADRRSEEDWFAP
                     QVFTRASEFLEATTSERPFFLTVDCYDPHAPWDPPEEYVDMYDEGYDGPEPFAPVDGS
                     SEYLTGRQIRRMSALYSGEVTMTDRWLGRFLDKMEELDLFDNTLLILLSDHGIAHGEH
                     GVVGKLPSTLWPEVTDIPFLIRHPEGRGAGTTSDYYASTHDVAPTILGFLEIEPPLPL
                     DGQDLSVMLEGGEPEARPHFTLGYHDHVFTRDEEYAMMSSNDGTETRLYDLREDPGMY
                     EDVAGDLPEIARRMFDDYVLEDAGGPLPRY"
     gene            2858..3106
                     /locus_tag="VNT12_06955"
     CDS             2858..3106
                     /locus_tag="VNT12_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45894.1"
                     /translation="MTVFRYGQQHFEEGEYVRRVEATPGDDLDPVVRIVEDLGTVEIP
                     CGKVDCRSNCLEYLVRATSRRNDYTLAECKISALVSII"
     gene            complement(3272..3814)
                     /locus_tag="VNT12_06960"
     CDS             complement(3272..3814)
                     /locus_tag="VNT12_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45895.1"
                     /translation="MILLRLVGIGAIYGVGFMFVKSLFPEPFVLLILQGGQSSEGDLT
                     TLALVYMGVGLLAGLIAGPIFGGALILRRGRSEGSGPRLVLSLALALLTGLISGILTL
                     IVYAVGILPGGGVLDPLRLIEDSNHPPGVPLLVAWTIARDLLPAGLAGLFLSPLAGGA
                     LQQLYAAERPPAQKRYSWEE"
     gene            3966..5126
                     /gene="queG"
                     /locus_tag="VNT12_06965"
     CDS             3966..5126
                     /gene="queG"
                     /locus_tag="VNT12_06965"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWI45896.1"
                     /translation="MRPVQDQAETRRVLEERAREAGFDLVGVARAEPLREGGERLREW
                     QEAGMAADMGYMHRPVELLSDPKKLQKSARSVVSLGVSYYPGDHPENEGGGRVARYAW
                     GLDYHQVIKGRLFRLREELEEELGVRIRARGFTDAVPLLERSAAQHAGLGFFGRNSCL
                     INNEIGSYFFIADLIVDLDLDPNPPGEGTCGRCTRCMDRCPTGAIKAPGVVDARLCIS
                     YLTIENRGEIPRHLRSKVGDWAFGCDVCQEVCPYNKTKASGSRWPEFSREAGSGPYLE
                     IEEVLKIRTDEEFEDRFGGTPLTRPGRAGLLRNCCVAAGNLKLARAVPALVRALRGDP
                     SPLVRGHAAWALGEIGGAKESLAEASETEKDTWCLQEIGLALGGGTTNATLG"
     gene            5139..5972
                     /locus_tag="VNT12_06970"
     CDS             5139..5972
                     /locus_tag="VNT12_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWI45897.1"
                     /translation="MADQINYYEVLGVPREASQAEIRNAYRNLAKERHPDHPGGSAEE
                     FSRLQEANAVLSDPDRRRQHDEAIDLAHAADQLAGLNLDFGSVEDELSAKRRERESGG
                     PSLGERLRGRFGRKEEDPDESLRRETRGSGRGSGRRTRGRYDVSEARWYEPHRFDPEP
                     VTLKSGAISFVAAFLAFIVIAQIGLWATAPEQVDPGPFSLVAALAPFMPILYSLVGLV
                     VAYFTFRAAGYAALALAFVAALVVGGQGPTGLLQFATVGIIVLLVVVYLGNRRESAAR
                     R"
BASE COUNT         1104 a         1824 c         1987 g         1135 t
ORIGIN      
        1 caccgaaggt aacggggatc tcaccggcct tcaacttcag ggtgatacgc tcgacggggc
       61 aggcgagtat ctcgtgctgg atcacgtacg ccgtaccgag ggtggggtgg ccggcgaagg
      121 gtacctcatt accaggggtg aagatgcgga cgtcgtagcc accttcggtc tcttcctccg
      181 acaaaacgaa ggcagtctca gagtagttaa tctccagggc tatcttctga agtctctcat
      241 ctggcaagcc ggcgccgcca cgaacgacag cgagttggtt gcccgcgtat ttctcttcgg
      301 cgaagacgtc cacgatgtag aaagtgaggt tcacggtcgg tcctaaccgt agtccgggtc
      361 gctgcgggat agccgcatta cgccgatgaa catcttgaag gccgtttcat agtcctcgtg
      421 cgtccatgag atgcgccggt cgtccacgcg ctccaggcct gggaagaaga ggacgctcgc
      481 ggcagagctg gcggtcgcga actcgatggt gaaggagtag ggcggctcag gtgcgtaagg
      541 actcaggtct cctacaccca ccgcgcgcag ggccgcctcc ctgatgcgct ctttggccac
      601 ctgcggggag aggcaccggg ccgtgtagcg gtcgacgacg gtcttcaccc gcgcagtctg
      661 cactccgggg tagagggatt ccgcctccgc gcaggccagg tcgtcaccgg tcacgaggcc
      721 gacgggaatg cccttgagcc cggcgagcgt cgcgttcatt cgcgcctcgc tgcacggctc
      781 gccgttcaac catactcccg tgacgacggt agggctgtcg aaggtgtggc tcaggacccc
      841 ttccgtgccg gcgcgggcgt ggtagccgac gaagaagacg aggtcggcgc cttcgaagcc
      901 ctgcatcatg gagtagggtt tgcgctggcc gacgatcacc tcagcccgcg ggtcgaggtc
      961 ctcgaggagg atattccgca tgccgtcgtg agcctcgttg accaggaatt ctgtgacgtc
     1021 ggcctgcgcc gcgccttcga tggcggcgtt cacgtcggcg gtcaggagcc tccgaaagcg
     1081 ctcgtactgc gagcggccag gtattacgtc ctgggagtgc gtcacccccg tcacgccctc
     1141 catatccgcc gagatgtaga tcttcatgac atcgcctcct ctcctggata ttcaggggtc
     1201 ggcacactac ggagtactgt ggtcttccgt gcctgaatca gtacctgggc agtggccctc
     1261 cggcatcttc taggacgtag tcgtcgaaca tacgtcgcgc gatttcggga agatctcccg
     1321 cgacgtcctc gtacatgcca gggtcttctc tcagatcgta aagcctggtc tccgtcccgt
     1381 cgttgctgct catcatggcg tactcctcgt ccctggtgaa gacgtggtca tggtatccga
     1441 gcgtgaagtg gggcctcgcc tcgggctctc cgccttccag catcacggag aggtcctggc
     1501 catccagcgg tagcggaggt tcgatctcga gaaacccgag tatcgtaggc gcgacgtcgt
     1561 gggtggaagc gtagtagtcg ctagtcgtcc ccgctcccct gccctccgga tggcggatca
     1621 ggaacgggat gtccgtaacc tcaggccaga gagtggaggg tagcttaccg acgacgccgt
     1681 gttccccgtg ggcgatgccg tggtcggaga gaagtatgag gagggtgttg tcgaagagat
     1741 ccagttcttc catcttgtcc agaaagcggc cgagccagcg atccgtcatc gtcacctcgc
     1801 ccgagtagag ggcgctcatc cgccgaatct gccgcccggt aaggtactcg ctgctcccgt
     1861 cgaccggcgc gaaaggctcg gggccatcgt aaccctcatc gtacatatct acgtactctt
     1921 cgggagggtc ccacggcgcg tgaggatcgt agcagtccac cgtaaggaag aaaggcctct
     1981 ccgatgtggt ggcttccagg aactcggagg cccgggtgaa gacctgcggg gcgaaccagt
     2041 cctcctccga tctgcggtcc gccgtattgg cgaagtaccg ctggaagtac gctctgctcc
     2101 ccgttcccaa aatggctctg ttcaccagcg cctgctcgac cttcccgggt gggcaggtat
     2161 agagtggttt gtagctgtct gtggtctgtc ctcggataaa atcgaaggct tcgaagccgc
     2221 gctggaagtt catagaggcg tcgtactgct gcaggttatc ggtgacgaac agcgtctcgt
     2281 agcccgcgct tcgcatagtc tcagcgaggg tgatctggtc gttggggatc ggctgccagc
     2341 cctgaagacc gacgtctatg cctttgtaac gtcgccagtc ctcgaaaggc caggtcctga
     2401 ggcccgtgtg tatggccctc ctcgcgcaga tcgtcggtat ggactcaggg tatgccctac
     2461 tgaagagcag gctctcctct gcgagagcgt ccaggttggg ggtctggatc ttctcgttgc
     2521 cgtaggcgcc gatgtgatcc ttcctcaggc tatccatgat gatcagcacc acgttggtgt
     2581 tcctgtccgc ggttggggtc ttgcctggga ggtcagggag tacgctcgta cttccgcacc
     2641 cggcgagcaa cgatgccccg gctacccccg cgccagacat cttcaagaac tctcttcgcg
     2701 ttaacgtccg cctcagtctc tcctcctgca taccgctcgc ttgcacagac aatcatagag
     2761 caagttaagt gggaagcctc atcgtacccg ggatctctgc gggcgaagca ttcggctgag
     2821 cgatgtgatc caggtcaagc cggtgtacaa tctgggcatg acggtctttc gttacgggca
     2881 gcagcacttc gaggaagggg agtacgtacg ccgcgtggaa gcgacgccgg gcgacgacct
     2941 cgaccccgta gttcgcattg tggaggactt ggggaccgtc gagatcccct gcggtaaagt
     3001 agattgccgc agtaattgcc tggagtactt ggtcagggcc accagcaggc gcaacgacta
     3061 caccctggcc gagtgcaaga tttccgcgct ggtaagcatc atatagcatt tcagctagtg
     3121 agcacgcctg cttcgcagcc ttgtcagctt gcctgtgtcc aagtcttggc tgactggctg
     3181 acaaggcggc gtagccgccg tgctgataag cgaaggcgcc agcctgagcg tgctgacaag
     3241 cgtaggccac aggccgaagc gtgcttaaag gctattcctc ccaggagtag cgtttctgtg
     3301 ccggtggtct ttcggcggcg tagagctgtt ggagcgcgcc tccagccagg ggggagagga
     3361 agagaccggc gaggcctgct ggcagaaggt ctcgggcgat ggtccaggcc acaaggagcg
     3421 gcactcctgg cggatggttg gaatcttcta tgaggcgtag agggtcgagg acgccgccgc
     3481 cggggaggat acctacagcg tagacgatga gggttaggat gccggagatg agccctgtca
     3541 gcagcgcaag cgcgaggctg agaactaagc ggggaccgga accttccgaa cggccgcgcc
     3601 tcaggatcaa agcgccgccg aatatggggc ctgcgatgag ccccgcgagc aatccgacgc
     3661 ccatgtagac gagggccagc gtggtcaggt cgccctcgct gctctgtccg ccctgcaaga
     3721 tcaggagcac gaaaggctca gggaacagcg atttgacgaa catgaagccc acgccataga
     3781 tggcacctat ccccaccaga cgcaggagga tcatggtcta cgtgccgccc ggtcggggaa
     3841 gataaggcat tgaaggctag tattcgtcat catgccctag accttaacct ccaacggccg
     3901 aaacgtcagc aagagctttc acgaggcgtc gtctttacgc gccgacgggt acaatcggca
     3961 gcagcatgcg accagtacag gaccaggcag agacgcggcg cgttctcgag gagcgggcgc
     4021 gagaggctgg cttcgacctc gtaggcgtgg cgcgcgctga gcctctgcgg gagggcggcg
     4081 agcgtctgcg ggagtggcag gaggcgggaa tggccgccga catgggctac atgcacaggc
     4141 ccgtggagtt actttcagat ccgaagaagc tccagaagag cgccaggagc gtggtctctc
     4201 tcggggtctc ttactatcct ggggaccatc ccgagaacga aggcggaggc agggtggcgc
     4261 gctacgcgtg gggcctcgac taccaccagg tcataaaagg gaggctgttc cggctgaggg
     4321 aggaactcga ggaagagctg ggcgtcagga tacgagcccg cggtttcact gatgccgtgc
     4381 cgctcctgga gcgttccgcg gcccagcacg cggggctagg ttttttcggg cggaactcct
     4441 gcctgataaa caacgagatc gggtcttact tctttatagc ggacctgatc gtggacctcg
     4501 acctcgaccc caatccgccc ggcgagggga cctgcggacg ctgtacgcgc tgcatggaca
     4561 ggtgccccac cggcgccata aaagcccccg gcgtcgtgga cgccaggctc tgtatctcgt
     4621 acctcacgat cgagaaccgg ggtgagatcc cccgccacct ccgttcgaaa gtgggtgact
     4681 gggcctttgg gtgcgacgta tgccaggagg tctgtcccta caacaagacg aaggcgtcgg
     4741 ggagccgctg gccggagttc tccagggagg ccgggtcagg accgtacctg gagatagaag
     4801 aggtcctcaa gataaggaca gacgaggagt tcgaggatcg ctttggtggg acccccctca
     4861 cgcgccctgg ccgggcaggc ctgttgcgca actgctgcgt ggccgcaggg aacctgaagc
     4921 tcgccagggc tgtccccgcg ctcgttcggg ccttgcgtgg ggacccttcg ccgctcgtac
     4981 gtggacacgc ggcgtgggcg ctgggcgaga tcgggggtgc taaagagtcg ctagcggagg
     5041 catccgaaac ggagaaggat acgtggtgcc tgcaggagat cgggctcgcg ctggggggcg
     5101 ggaccacgaa cgctaccttg ggttagaata aggaagcgat ggcggaccag atcaactact
     5161 acgaggtgct cggcgtccca cgcgaggcct cccaggccga gatccggaac gcttaccgca
     5221 acctggcgaa ggagcgtcac cccgaccatc ccggtgggag cgcggaggaa ttctcccggc
     5281 tgcaggaggc caacgcggtc ctctccgacc cggaccgtcg tcgtcagcac gacgaggcaa
     5341 tcgacctggc ccatgcagcc gaccagctcg ccggcctcaa cctggacttc ggctccgtgg
     5401 aggatgagct ctccgccaag cgcagggagc gcgagtcagg cggtccctcc ctgggggagc
     5461 gcctgagggg tcgcttcggc agaaaggaag aggaccctga cgagtctctg cggagagaga
     5521 cgcgcggctc cggccgcgga agcgggcgta gaaccagggg ccgctacgac gttagtgagg
     5581 cccgctggta cgaaccccac cgcttcgacc ctgagcctgt aaccctcaag agcggcgcca
     5641 taagcttcgt cgctgccttc ctcgcattca tcgtgatcgc ccagatcggg ctgtgggcaa
     5701 cggccccgga gcaggtcgac cctggtccat tctctcttgt agctgccctg gccccgttca
     5761 tgcccatcct gtactcgctg gtcgggctgg tcgtggctta cttcaccttc agggctgcgg
     5821 gttatgcggc gctggccctg gccttcgtgg ccgccctcgt cgtcggcggc caggggccga
     5881 cggggctttt gcagttcgcg accgtgggca tcatcgttct gctggtagtg gtttacctcg
     5941 gcaaccgccg cgagagcgcc gcccggcgct gatcccgacc ccttagaagt agcttttggc
     6001 cataaccacc tcggaactgg agcgctttgt ctccgacttc gagagacgca
//
LOCUS       DATMJA010000293         4423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_749759, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000293 DATMJA010000000
VERSION     DATMJA010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749759"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1389)
                     /locus_tag="VNT12_06975"
     CDS             complement(295..1389)
                     /locus_tag="VNT12_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45898.1"
                     /translation="MATVTVLGSGMMATALAWPLSDNGHEVRLVGTHLDRDVIDSIKE
                     SGVHPKLDLKVPEGVRAYQLEEAEEAFEGAQIVMSGVNSFGVRWASEQLALLLRQGVM
                     VIAVTKGMDADEDGNLRTLPEVLAEQVPQDVREQVSWSAIVGPSIAGEVAVRRDTCVV
                     FTGEDAGVLEKLAATFRTDYYHVWTSTDFVGCEVCAAIKNCYAFGAGFMKGILDRKGE
                     SEAKYRNFNYGAALFGQATREIGLWMKLLGGKPETPYGLPGVGDMFVTSMGGRNVKAG
                     RFVGAGVPFSEVQDEQMKGVTLEGVAAIRVVGGALPKLMERGVISQEDFPLMRHLYEV
                     VALDEPLDMPWSAFFGGESRGKAEARTITK"
     gene            complement(1441..3039)
                     /locus_tag="VNT12_06980"
     CDS             complement(1441..3039)
                     /locus_tag="VNT12_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWI45899.1"
                     /translation="MAEMKSGSTAEGPYLLGMDIGTGGVRVGIFDRKGTPIVFEGVEF
                     ETHHPRPGRAEQDPETWWSCLVTATRRALEEGGVSPREILGIGVDATASTLLAVDEQG
                     EPLLPAIMWMDVRASEEAARVEETGDPALKYSGYGAVSAEWGLPKVLWLKENEREVYD
                     GAGRVCDCLDWINHRLTGEWASSINVVSTKYFYDRDEGGWPESLYGTVGADDALEKFP
                     PAVLDLGSVVGGLRREAAEELGLTTGTPVAQGALDAYSGALGLGVVEPGKLALITGSS
                     HVMIGQSADPVHGQGLWGAYTDAMIPGQYTVEAGQVSTGSVVAWFKNRYAGEAVTAEA
                     QKRGVDPYVVLTEMAEKAPIGSDGLVVLDYFQGNRSPHTDPLARGMMWGLSLAHTPGH
                     MFRAIIEGICYGTEHILRTMRARDFEPRITVVSGGPTKNGLWMQIHADVSNLPISFTR
                     VKEGSVLGSAMLAAVGAGIYPDIPAASDNMVHTEKTIYPNHEAHEAYNFYVDRYIETY
                     PRMRELMHEMTRHEAKCNRAPADA"
     gene            complement(3032..4027)
                     /locus_tag="VNT12_06985"
     CDS             complement(3032..4027)
                     /locus_tag="VNT12_06985"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWI45900.1"
                     /translation="MSISVVWPGQEGVEVKPRDVPPPGLGELKIRIEASGLCGTDLHI
                     ASGEYPFARPGVTIGHEFAGTIVEVGPDVEGFGVGERVVVDPNIPCRTCEYCHDSRPH
                     LCVRPQALGVTLNGGLSEFAVLPAPQAYRVPEGLPPEAAALTEPLACTLHAVDVGGLR
                     PGECALVLGAGPIGVLCAALLVAAGSSEVLISEPDPGRRDRVREVGAVPVEPESLTEG
                     TADRVFECVGRPETMRTAVETAKPGGTVVWVGVAPPEAEVSIRPYDVFRRELTIRGTY
                     TNPFTMGRSLALLASGRIDWRSIVTHRIPIARFEEAWETHREGTGLKVCVYPGDG"
BASE COUNT          773 a         1535 c         1255 g          860 t
ORIGIN      
        1 cctctagtaa gggatatagt ggctcgcttc tatgtctcat agtccctgcc tccagctcca
       61 aggttggtag aggtacctta gtccctgact aatctcgtgc ctggcgcttc gtcgggctga
      121 atccctccac tgctctgcac aatccgctcg tagtctttga ttaaacggcg gttccgcacc
      181 agccatccaa atgtccgctc cgcgatccac ctatgccgct gaaccatgcc ggccccacca
      241 cgatcagctc gaagggccgg tccccgacac tctccaaata caatctctcg tggttcactt
      301 cgttattgtt cttgcttcag cttttccgcg gctctctccg ccgaagaacg cgctccaggg
      361 catatcgagg ggctcatcta gggcgacgac ctcatacagg tggcgcatca acgggaagtc
      421 ttcctgggag atgacgccgc gctccatcag cttcggcagc gcgccgccga cgaccctgat
      481 ggcagccact ccctcaagcg tcacaccctt catctgttca tcctggacct cagagaacgg
      541 tacgcctgcg ccgacgaaac gtcccgcctt cacgttgcgc ccgcccatgc tggtgacgaa
      601 catgtccccg actcccggca gcccgtaggg cgtctcgggc tttccgccaa gaagcttcat
      661 ccacagcccg atctcgcgcg tcgcctgccc gaagagcgcc gctccatagt taaagttgcg
      721 gtactttgcc tccgactcac cctttcggtc caggatgccc ttcatgaaac cggccccgaa
      781 ggcgtagcag ttctttatgg ctgcgcacac ctcgcacccg acgaagtctg tgctagtcca
      841 gacgtgatag tagtcggtac ggaaggtcgc agctagcttc tctagaacgc ccgcgtcttc
      901 gccggtgaag accacgcagg tgtcccgtcg cacggccacc tcgccggcta tcgacgggcc
      961 gacgatggct gaccaggaga cctgctctct cacgtcctgg ggtacctgtt cggccaggac
     1021 ctcgggcaga gtccttagat tgccgtcctc gtcggcatcc atccctttgg tgaccgcgat
     1081 caccatcacg ccctgtctca agagcaacgc cagttgctcg ctggcccagc gtaccccgaa
     1141 agagttgaca ccgctcatca cgatctgggc gccctcgaaa gcctcctcgg cctcctctaa
     1201 ctgataggcg cgcactcctt ccgggacctt gaggtcgagc ttcgggtgta cgccactctc
     1261 cttgatgctg tcaatgacgt cgcggtcgag gtgcgtgcca acgaggcgca cctcgtggcc
     1321 gttgtccgaa agcggccacg caagcgccgt cgccatcatg cccgatccca agacggttac
     1381 cgttgccatc ttcctcctct atctacgtcg aaatagactg cctggacccg gctgcgagac
     1441 tcaagcgtcc gctggggccc tgttgcactt tgcctcgtgt cgcgtcatct cgtgcattag
     1501 ctctcgcatc cgtggatagg tttcgatata gcggtccacg tagaagttgt atgcttcgtg
     1561 ggcctcgtgg tttggatata tggtcttctc ggtgtggacc atgttgtccg aggccgctgg
     1621 gatgtccggg tagatcccgg ctccaaccgc ggccagcatc gccgatccca gcaccgagcc
     1681 ctccttgacc cgcgtaaagg agatcgggag gttcgagacg tcggcgtgga tctgcatcca
     1741 cagcccgttt ttcgtggggc cgccggagac gacggttatc ctcggctcga agtctcgggc
     1801 gcgcatggtg cggaggatat gctcggtacc gtagcagatg ccctcgatga tcgcccggaa
     1861 catgtgtccg ggggtgtggg cgagcgagag accccacatc atgccgcgcg ccagcgggtc
     1921 ggtatggggc gacctatttc cctgaaaata gtcgagcaca acgagcccgt ccgagccgat
     1981 gggtgccttt tcggccatct ccgtgaggac cacgtaaggg tctacaccgc gcttttgggc
     2041 ctctgcggtc acggcttcgc cggcatagcg gttcttgaac caagccacta ccgagccagt
     2101 ggagacctgc ccggcctcga cggtgtactg gcccggaatc atggcgtcgg tataggcgcc
     2161 ccacagtccc tggccgtgga ccgggtcggc ggactgcccg atcataacgt gtgaggaacc
     2221 cgtgatgagg gccagcttgc cgggttcgac caccccgagc ccgagcgccc cagagtaagc
     2281 gtccagggct ccctgggcca ccggcgtgcc ggtcgtcaga ccaagctcct ccgccgcctc
     2341 ccgcctcagc cccccgacca cgctgccgag gtcgagcacg gctggcggga atttttccag
     2401 cgcatcgtcg gcgcctaccg tcccgtagag gctttccggc cagccgcctt cgtcacgatc
     2461 gtagaaatac tttgtactga ccacgttgat ggaagatgcc cactcaccgg tcagccggtg
     2521 gttaatccag tcaagacagt cgcagacacg tccggctcca tcgtagacct cccgctcgtt
     2581 ctccttgagc cagagaacct tgggcagacc ccactcggca gagaccgcgc cgtacccgct
     2641 gtacttgagc gctgggtcgc ccgtctcctc tacccgagcc gcctcctcgg aggcgcgaac
     2701 gtccatccac atgatggcgg gcagcagggg ttcgccctgc tcgtccacag ccagcagcgt
     2761 ggatgccgta gcgtccacac cgatgcccaa tatctcccgc ggcgagacgc ctccctcctc
     2821 caacgcccgt cgtgtcgccg ttaccaggca tgaccaccac gtctcgggat cctgctccgc
     2881 tcgtccgggg cgggggtggt gcgtctcgaa ctctacgccc tcgaagacga tcggcgtccc
     2941 cttgcggtcg aagatgccca ccctaacgcc gcccgtcccg atatccatac ccagcaggta
     3001 tggaccttcg gctgtggatc cggacttcat ctcagccatc gcccggatat acgcagacct
     3061 tcaagccggt tccttcgcgg tgcgtctccc aggcttcctc gaaccgggcg atagggatac
     3121 gatgcgtgac gatagacctc cagtctatgc ggccggaggc gagcagggcc agcgagcgtc
     3181 ccatcgtgaa cgggttggtg taggtaccac ggatggtcaa ctcgcgccgg aagacatcat
     3241 atggccttat ggagacctcg gcctctggtg gagccacgcc cacccacacg accgtcccgc
     3301 ccggtttggc ggtctccacc gcagtacgca tcgtctcggg gcgaccgacg cactcgaaca
     3361 ccctgtccgc agtgccttcc gtcaacgact ccggctccac gggcacggcg ccgacctcgc
     3421 ggacgcggtc ccgcctgccg ggatcaggct cgctgatgag tacctcagac gagcccgccg
     3481 ctacgagaag cgcggcgcac agaaccccta tcgggccggc gccgaggacc agggcgcatt
     3541 cccccggacg taggcccccc acgtccaccg cgtgcagcgt acatgccaga ggttctgtga
     3601 gtgcggcggc ctcaggtggt aagccttccg gcacccggta tgcctgcggc gcgggtagca
     3661 ccgcgaactc ggacagcccc ccgttcaggg ttacgccgag ggcctggggc cttacgcaga
     3721 ggtgcggacg cgagtcgtga cagtactcgc aggtgcggca cgggatgttg gggtctacga
     3781 cgaccctctc ccctacgccg aagccttcca cgtccggtcc gacctccact atggtccccg
     3841 cgaactcgtg cccgatggtt acgcccggcc gggcgaacgg gtactcaccg gatgcgatgt
     3901 gaaggtcggt cccgcacaat cccgaagcct cgatccggat cttcaactcg ccaagacccg
     3961 gtggcggtac gtcacggggc ttaacttcta ccccttcttg cccgggccag accacactta
     4021 tgctcatgat ttaaccatcc cctgtgtctt cgaatttcac actctctcct tcacctccga
     4081 caaccgcact tagagtcagc gcgtaccaag tagcgcctga tagcgaggcc cgcggttgcc
     4141 atcggcaacg ccaccgctgt ccctatcgcc gtcagctgac cccggtctgc gttatcagct
     4201 gggaggcgat cgccagcgag gtctgactac ctggtcccgc gaacgtttcc gagacgcaac
     4261 acgatggcta catctctcaa agctacacgt ccttagaacg cgagcacttc cgagaatcgg
     4321 attacgtcca gaaatcgtcc gaaagtctcg gaggtgcctg cggcgtagcc ctacgaaccg
     4381 tcgaaagggt acttttcggc ccacttcttc ctccgtaaac cgg
//
LOCUS       DATMJA010000294         7446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_97961, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000294 DATMJA010000000
VERSION     DATMJA010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97961"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..648
                     /locus_tag="VNT12_06990"
     CDS             223..648
                     /locus_tag="VNT12_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45901.1"
                     /translation="MEASVSEIHRYPLRPEGFEEPLFPTRESRLAVLDHLLRAPWLSR
                     ASEIEERGGQAAHARWEPVEWENRHGRVRIVWASGRRRCRKRRRVIEVLGRWREVRAW
                     WDETQAKDRSLARLLLSDGSVVDLARESDGEWFLVGVTD"
     gene            645..3923
                     /locus_tag="VNT12_06995"
     CDS             645..3923
                     /locus_tag="VNT12_06995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWI45902.1"
                     /translation="MNGESSDGFVHLHVRSGFSFGFGVARPEELVEAAARMGMSSLAL
                     TDRDGLYGIPRFLEAADEAGILPIVGAQVSTAGGHVVLLAESIEGYRSLCRLITDYRT
                     SSEDRRRPACPLGKILEHREGLICLTGAVPFGLIPSLILAGQRKEATEVLRRLHEAFG
                     DRLYVELTDDETATGRRSMGKVAAFARDAGVPIVAAGDVAYLFPSDHRLHDVLVAAAN
                     LTALPGQEYRPTDRLHLRSGKEVQRLFEGYPDALANVADVARWCAGAVKLSGKVHMPA
                     AILPTGKTAGKMLLELTLAGARRRYGRRAGDGPTAEELKSRLGRELRCIHALGFAPYF
                     LIAHEAKEIAKSKGIPVTGRGSAANSLVSYCLGLSEPEPFSNRLFFERFLHEGRKDPP
                     DVDLDFCSVRRDEVRFEMIHRYERRGVAEAATVQTMSLRGAVRVAARALGHSPQEIDT
                     LSRQVPTRFQDRDRIYAGLSGWEESLAEPAMYGHPLQDTERYRLLLELSAGLAGRIKE
                     GGTHSGGMVFGTAEHHLSELVPLEPSGTEGLLRCQYDKDDLERVGLPKLDLLGLRMHT
                     ALHKAGELASRRLGRRVDPYDLPPDDKRTYALIRTGRNAGMFQLESPGQMHLSQRLGP
                     RRLADLVAQISLFRPGPVRGDLVTPYVKRRNGREPYSVILPELEEVLRPTYGVLVFQE
                     QVLAVANAVAGFTLAEGDLLRRAMTKDRGPGAMRELRLEFMRRARENGVLAAKADEVF
                     SWMEGFSVYGFSAAHAASFAQLAYASAYMRRHYPAEFFAAVLDSQPMGFYSPRLLLNE
                     ARRIGLSVLPPDVHLSGEGFAVEEDGSALRVGLRYCKGLSEKAISSIVAERRKKPFVS
                     VADLYGRTAVEKDALENLIRGGFLDSLAGGSERYRLLGESAALPKKRRRGRQGEIPIE
                     HPASWWAMRERRSVGHLPLTDTARERMEWEMLGLNIHRHPLVPHRDALRELRVVPSVG
                     IRALPHGTSACAAGLIECLQSPPTKSGYRVYFLLIEDEWGLLQTTIFRSVYERCGDIL
                     HHEGAFLVEGRVEQTIEKGFAFLVHNIESLRDVLFEARVPVPRVASAPGAFLRAGRRS
                     RKAG"
     gene            complement(4071..4490)
                     /locus_tag="VNT12_07000"
     CDS             complement(4071..4490)
                     /locus_tag="VNT12_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI45903.1"
                     /translation="MKITDADHTNWRVKDVERSLGFYRDVLGLEPFGLDEYERGEHPL
                     VSLRVTPGFILHLRPDPTFETVSTGGYDHLALVVEGTNLDVLAEHLTEAGVEIERRSE
                     NVVGARGSGEALYVRDPDGYLIEFKLYTNESGSPRAE"
     gene            complement(4739..5107)
                     /locus_tag="VNT12_07005"
     CDS             complement(4739..5107)
                     /locus_tag="VNT12_07005"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HWI45904.1"
                     /translation="MNDEPLAQIERKILRWDGVFKKRDEDGPGGIGVTGYRYGDPEKG
                     GPQIGHVHDDGHADFRFPREVRDELIRTGRAIPHPAFPNSRTTASYRIQSAEDVAEAL
                     ELFRMNYERRKQRNGPAKAG"
     gene            complement(5299..5730)
                     /locus_tag="VNT12_07010"
     CDS             complement(5299..5730)
                     /locus_tag="VNT12_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWI45905.1"
                     /translation="MTGANLITHDKEEDVETLERIFLRGVILPGQERLELREFDRPEP
                     GHGQVLVKMKASGLCGSDLRAIYHKHTGSGAERYQNVIAGHEPSGRVEAVGPGVGGFE
                     LGDWVVVYHILGCGRCVKLAQDATRGSRSCGGNCAGTAVGF"
     gene            5825..6568
                     /locus_tag="VNT12_07015"
     CDS             5825..6568
                     /locus_tag="VNT12_07015"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HWI45906.1"
                     /translation="MREGLARRRVGEEGFRWRGEDVSRIEGFSDAVFAFAVTLLVVSL
                     EVPKTFDELLATMRGFFAFAICFALLLSVWYDHYKFFRRYGLKDTPTRWLNSTLLFLM
                     LLYVYPLKFLFTMLIDEWLGFGNSEMIESSQIPLLMVIYGAGFVAIQLVFFLMYLRAY
                     SLRTALELDSQELSVTREEMQGFFLNILVGLTSVAIAALGGVGATSWAGLVYLLVFPL
                     QTINSRAMGSRRRRYRDALTKAGAGGEGP"
     gene            6627..7364
                     /locus_tag="VNT12_07020"
     CDS             6627..7364
                     /locus_tag="VNT12_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWI45907.1"
                     /translation="MTEQARRTRLRDRAADQLLEMVISGGLEPGERLPPERELCAHLG
                     VSRTVVREALNLLEARGLISIEHGRGAVVSGGSPRAVRDTLGLLLRVQPKTLWELLEI
                     RKILEVEVAGLAAERAGPDDVATMRLQLDKMRDFIDAPEGYVDADVEFHALLARATCN
                     EVLLTMLEPVVELLRASRRVSAARPGNALRALGEHEEILRRVEARDAGGAREEMRAHL
                     AKTAEDIEAAISEGVLDAGELLEKERV"
BASE COUNT         1313 a         2278 c         2434 g         1421 t
ORIGIN      
        1 atactatttt gccgtagact gcaaacaata gtttgtggta gcgtaaagag aagaaaagca
       61 aagcaggagc gcccccaccc cgggcctcgc tggccggccg gcgtccaacc agtaccggcc
      121 tgcgagggtt agaggaggcg aggacgcctg ctccccggat tatatccgga ggcgcgggtt
      181 tctcatcccc cggctcaagc gctccgtgga gggaggagag agatggaggc cagcgtctcg
      241 gagatacacc gctatccctt gcggcccgag ggttttgagg agccgctgtt ccctacccgt
      301 gagtcgaggc tggcggtcct tgaccacctg ctgcgtgccc cctggctctc gcgggcctcg
      361 gagatagaag agaggggagg acaggctgca cacgcccgat gggagccggt cgagtgggag
      421 aaccgccacg ggcgtgtgcg gatcgtgtgg gcttcaggcc ggagacggtg ccgcaagagg
      481 cgccgcgtca tcgaggtcct cggccgctgg cgggaggtcc gggcgtggtg ggacgagacg
      541 caggcgaaag acaggagcct cgcgaggctc ctcctctccg acggctcggt cgtcgacctg
      601 gcgcgggaga gcgacgggga gtggttcctc gtaggggtaa cggattgaac ggagagtcct
      661 cagacggctt cgtccatctg cacgtcagga gcgggttctc cttcggtttc ggggtcgcga
      721 ggccagaaga gctcgtggag gccgcggcga ggatggggat gtcctctctg gcgctcacgg
      781 accgcgacgg actctacggc atcccccgct tcctcgaggc tgcggatgag gctggcatcc
      841 ttcccatcgt cggggcccag gtctcgacgg cgggtggaca cgtcgtgttg cttgccgagt
      901 ccatagaagg ctaccgttcc ctgtgccggc tcatcacgga ttacaggaca tcttctgagg
      961 acaggcgccg tcccgcctgt ccgctgggga agatcctgga gcatagagaa ggtttgatct
     1021 gcctgaccgg agccgtcccg ttcgggctga tccctagcct gatccttgcc ggacaaagga
     1081 aagaagcgac cgaggttctg cgccgcctgc atgaggcatt tggggaccgg ctctacgtcg
     1141 agctgaccga tgacgagacg gcgaccgggc ggcggagcat gggaaaagta gcggcctttg
     1201 cacgggacgc tggcgtaccg atcgttgccg ctggcgatgt agcttacctc ttcccctctg
     1261 accacaggct gcacgacgtc ctcgtggccg ccgcgaacct cacggcgctc ccaggccagg
     1321 agtacaggcc gaccgaccgg ttgcatctca gatcagggaa ggaggtacag cggcttttcg
     1381 agggctatcc cgacgccctg gcaaacgttg cggatgtcgc gcggtggtgc gcgggggcgg
     1441 tgaagctgtc gggcaaagtc cacatgccgg cggcgatcct gcctacgggc aagacggctg
     1501 ggaagatgtt actggagctg acgctcgcgg gcgccagaag acgctacggg agaagagcgg
     1561 gggacggtcc cacggcggag gagttgaagt cgcgactcgg acgagagttg cgctgtatcc
     1621 acgctcttgg gttcgcgccg tacttcctga ttgcgcacga ggccaaagag atcgcgaagt
     1681 caaagggcat ccccgtcacg ggccgaggaa gcgccgcgaa cagcctggtc tcatattgtc
     1741 tggggctgag cgagcccgag cctttctcga accggctatt cttcgagcga tttctgcacg
     1801 agggacgcaa ggacccgccc gatgtagacc tggacttctg ctccgtaagg cgtgacgagg
     1861 tgcgcttcga gatgatccat cgctacgagc gtcgaggcgt cgcagaagcg gcgacggtcc
     1921 agacgatgtc actgcgagga gctgtgaggg tggcggcgcg ggcgctcggg cattctccgc
     1981 aggagatcga caccctcagc cgccaggtcc ccacccgctt ccaggaccgc gacaggatct
     2041 acgccggcct ctccggctgg gaggagtcgc tcgccgagcc ggccatgtac ggccatcctc
     2101 tccaggacac ggagaggtat cgtctcctgc ttgagctctc ggccggactc gccggccgca
     2161 tcaaggaggg aggcacccat agcgggggta tggtgttcgg gacggcggag catcatcttt
     2221 cggagctcgt gccgctggag ccttccggga cggaggggct cctgcgctgc cagtacgaca
     2281 aggacgacct ggagcgcgtg gggctcccga agctggacct gctcggcctg aggatgcaca
     2341 cggcgctgca caaggccggg gagttggctt cgaggcggct ggggcgcagg gtcgaccctt
     2401 atgacctgcc gcccgacgat aagaggacct acgccctcat aaggaccggg cgtaacgcag
     2461 ggatgttcca gctggagagt ccgggacaga tgcacctgag ccagcggctc gggcccagga
     2521 ggctcgctga cctggtagca cagatctcac tcttcagacc tgggcctgtg agaggagacc
     2581 tcgtcactcc ctacgtgaag cgccggaacg gcagggagcc gtactcggtg atcctacccg
     2641 agctagagga agtactgcgt cccacctacg gcgtcctggt cttccaggaa caggtcctcg
     2701 ccgtcgcgaa cgccgtcgca ggcttcaccc tggccgaggg cgacctcctg cgacgcgcca
     2761 tgaccaagga caggggaccc ggcgcgatgc gtgagctacg cctggagttc atgcggcggg
     2821 cgagagagaa cggggtcctc gccgccaaag cggacgaggt cttctcgtgg atggagggct
     2881 tctcggtcta cggtttctct gccgcgcacg cagccagctt cgcgcagcta gcctacgcga
     2941 gcgcgtacat gaggcgccac tacccggcgg agttcttcgc tgccgttctc gactcccaac
     3001 ccatgggttt ctacagcccg aggctgctcc tgaatgaggc gcgtaggatc gggctcagcg
     3061 tgctcccgcc cgacgtccac ctctccggag aaggcttcgc tgtagaagaa gacggctctg
     3121 ctctccgggt tggcctcagg tactgcaagg ggctatctga gaaggccatc tcctccatcg
     3181 tagccgagcg gcgcaaaaaa ccgttcgtct ccgtcgccga cctttacggg aggacagccg
     3241 tcgaaaaaga cgcgttggag aacctgatca gaggaggctt cctcgactct ctggctggag
     3301 ggtcggagag ataccggctg ctcggcgaga gtgcggctct tccgaagaag cgcaggcgtg
     3361 ggaggcaagg ggagatccct atagagcatc ctgcgagctg gtgggcgatg cgggagagga
     3421 ggagcgttgg gcatctcccc ctcactgaca cggcgagaga gaggatggag tgggagatgc
     3481 tcggcctcaa catccacagg cacccgctcg tgcctcaccg cgatgcgctc cgagagctcc
     3541 gggtggttcc gagcgtgggg atcagggctt tgccgcacgg caccagcgcc tgcgcggcgg
     3601 gtttgatcga atgcctccag agtcccccga cgaagagcgg ataccgcgtg tacttcctgc
     3661 tgatcgaaga cgagtggggc ctgctccaga cgacgatctt ccgctccgtc tacgagaggt
     3721 gcggcgacat cctgcaccac gaaggagcat tcctcgtaga aggcagggta gagcaaacca
     3781 tcgaaaaggg cttcgccttc ctcgtccaca acatagagag cttgcgagac gttctattcg
     3841 aagccagggt gcctgtgccg agggtggcgt ctgctccagg agcgttctta cgtgccggga
     3901 ggagaagtcg gaaggctggg taagaccttc acaaacatgt atcttgctca atcttgtgag
     3961 ctgctacgct tcttttcgtg ccagtcctaa accagctcgg gttgcctttc ggctaccgga
     4021 caccttctca acaaaccctg aagctcctat cttcgtcaac ccgtcgtacc tcattcggcg
     4081 cggggagacc cggactcgtt ggtgtacagc ttgaactcta tcaggtagcc gtcggggtct
     4141 cgcacgtaca gggcttcacc cgatcctcgg gcgcctacga cgttctctga cctgcgttcg
     4201 atctccacgc ccgcttccgt gaggtgttcg gccagcacat ccaggttcgt accttcgacc
     4261 accagggcga ggtgatcgta acccccggtg gagacagtct cgaaggtggg atccggcctc
     4321 agatgcaaga tgaacccagg cgttacccgc agcgagacga ggggatgctc tccgcgttcg
     4381 tactcgtcga ggccgaacgg ctcgagaccc aggacatcac gatagaatcc tagagacctc
     4441 tcgacatcct ttacccgcca gttggtgtgg tcagcatcgg ttatcttcat cgtctttctg
     4501 agtcctttcg tagtgcttcc taccagtcgt ggcactgtat ctccgttggt cagaggtgtc
     4561 ggccgcgact agcccctcag gccgcttccc accagcgaga tcgccagtcc cgtccacagc
     4621 aggtggaagt cgcggtggcg caggggacga aatgccgtgg gcaccgctcc cagcacggtt
     4681 actaccccac catcgcgaac cgcgtcgccc gtactattcg catgcctacc actctactct
     4741 atccggcctt cgcaggaccg tttcgttgct tccttcgctc gtaattcatg cggaacagtt
     4801 ccagggcttc tgccacgtct tccgcgctct ggattcggta gctcgctgtc gtccggctgt
     4861 tcggaaacgc gggatgaggg atcgcccgcc cggtgcggat caactcgtcc ctgacctctc
     4921 tggggaatcg gaaatccgcg tggccgtcgt cgtggacgtg cccgatctgg gggcctcctt
     4981 tctcgggatc tccgtacctg tagccggtga cgccgatccc acctggacca tcctcatccc
     5041 tcttcttgaa gactccatcc cagcgcaaga tcttgcgctc tatctgcgcc aacggttcgt
     5101 cgttcatctg tttcttcctc accctgtccg accccatatt acttgcaggt tgcgaacagg
     5161 ttcgaatctc cttgccagca tttcaacttc ttgtttttgc tgatcgcttg tagccgtatg
     5221 cgcgattcgc ggtaggtcga ttcctctgca aatcgcttta gcggtcccta tacactgcgt
     5281 gtctccgtcc gaacgacttc agaacccgac cgccgtaccg gcacagttcc caccacaaga
     5341 ccgggaacca cgggttgcgt cttgggctag ctttacgcag cgcccgcagc ccaggatgtg
     5401 gtatacgaca acccagtcgc ctagctcgaa accgcccacg ccgggtccaa cggcctcgac
     5461 ccttccggat ggttcgtggc ccgcgatgac gttctggtag cgctccgccc ccgagcccgt
     5521 gtgcttgtgg tagatggcgc gcaggtcgct cccgcagagc cccgaggctt tcatctttac
     5581 gagcacctgg ccgtggcccg gttcagggcg gtcgaactct ctcaactcta acctttcttg
     5641 tccgggtagg atcacccctc gcaagaagat cctctccaaa gtctctacgt cctcttcttt
     5701 atcatgggtg atgagattgg ctcccgtcaa ggtgcgatcc tgtgcgccct ggggtcgctc
     5761 ctttatcatg gtgtccgagc cgggtggaga tcggctgacg tcgagcggga gtgaacgggc
     5821 ggtgatgcgc gaaggattgg cgcggaggcg agttggcgag gaaggcttcc gctggcgcgg
     5881 ggaggatgtc tcgcgcatag agggcttctc tgacgccgtc ttcgccttcg cggtcacact
     5941 gctggtggtc tccctggagg tccccaaaac cttcgacgag ctgctcgcaa ccatgagagg
     6001 cttcttcgcg ttcgcgatct gcttcgcgct gctcctcagt gtgtggtacg accactacaa
     6061 gttcttcaga cgctacggcc tgaaggacac ccctacccgg tggctgaact cgacgctgct
     6121 cttcctcatg ctcctgtacg tctaccccct gaagtttcta ttcaccatgc tgatagacga
     6181 gtggttgggt ttcggcaaca gcgagatgat agagtcttcc cagataccgc tcttgatggt
     6241 catctacggg gcaggcttcg tcgctatcca gctcgtgttc tttctgatgt acctgcgggc
     6301 ctactcgctg cgcaccgccc tggagctcga ctcgcaggag ctctcggtga cgcgcgagga
     6361 gatgcagggc ttcttcctca acatcttagt cgggctgact tcggttgcca tagccgccct
     6421 cggaggtgtc ggggcgacct cctgggcagg gttggtctac ctgttggtct tcccgctgca
     6481 gacgattaac agtcgcgcta tgggctcccg taggaggagg tacagggatg ccctgaccaa
     6541 agcgggagcc ggcggcgaag gaccgtgaag cacccggcga tcgtcgcgtt tgacaggcat
     6601 ctcgattggt atgacaatgt cttaaggtga cggaacaggc acgacgaacc aggctcaggg
     6661 accgggcggc ggaccagttg ctggagatgg tgatctcggg cgggctggag ccgggagagc
     6721 gtcttccgcc cgagcgtgag ctgtgtgcgc atctcggggt gagcaggacg gtcgtaaggg
     6781 aggccctcaa cctgctggag gcccgcggcc tcatcagcat cgagcatggc cgcggcgccg
     6841 tggtaagcgg cggtagtccg cgggccgtgc gcgacacgct cggtctgcta ctgcgcgtac
     6901 agccgaagac gctatgggag cttctggaga tacgcaagat ccttgaggtc gaagtcgccg
     6961 gcctcgcggc ggagcgtgcc gggcccgatg acgtagcgac tatgcggcta cagctcgaca
     7021 aaatgcggga tttcatcgat gcgcccgaag gctacgtcga cgccgacgtc gagttccatg
     7081 cgctgctggc gcgggcgacc tgcaacgagg tcttgctgac catgctcgag cccgtggtcg
     7141 agctgctgcg ggccagccgg cgggtgagcg ccgccaggcc cggcaacgcg ctgcgggcgc
     7201 tgggggagca tgaggagata ctgcgccggg tcgaggcgag agatgccggg ggagcgcggg
     7261 aggagatgcg cgcccacctc gcgaagacgg cggaggacat cgaggccgcc atcagtgaag
     7321 gagtgctcga cgccggggag ctcttggaga aggagagggt atgaaggctc acgagctatt
     7381 gtccgggctg gggttgcccg gacgcgacct ctacgatctg cccgactcgg agaagcgttt
     7441 ccctga
//
LOCUS       DATMJA010000295         9290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_690564, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000295 DATMJA010000000
VERSION     DATMJA010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690564"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(525..1124)
                     /locus_tag="VNT12_07025"
     CDS             complement(525..1124)
                     /locus_tag="VNT12_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWI45908.1"
                     /translation="MPVSGMTALNGLRDAGKIQPEQKVLIIGAAGGVGTYAVQLAKAF
                     GAVVTGVCSRSKAELVWAIGGDEVIDYTREDFTDGTRRFDLILDTAGRRPLSQLRRAL
                     TPQGTLVIVGGEGGDRWLGGFQRQIFAPLRSRFSEQKLLGLMYKQRQQDLLTLKDLIE
                     AGKLTPVIDRTFPLKEAPQAIRYLEQGHARGKVVVTVEK"
     gene            complement(1276..1503)
                     /locus_tag="VNT12_07030"
     CDS             complement(1276..1503)
                     /locus_tag="VNT12_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45909.1"
                     /translation="MKAIVQDSYGSANVLELGEVEKPQPRDDDLLIRVHAAGVDLGVW
                     HLMTGLPYLVRVMGFGLRKPKTGRSWHRLRR"
     gene            1667..2527
                     /locus_tag="VNT12_07035"
     CDS             1667..2527
                     /locus_tag="VNT12_07035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI45910.1"
                     /translation="MERSLREQNRRSWNAVVGAHESHRGNLAAFLRAGGTTLFPEERE
                     LLGNLAGQTVAHLQCNWGGDSLSLATLGASVVGVDLSDQAVRSARQLSERAGIPACFE
                     HADVYDWLAAAARRGRRFDVVYSSYGVICWLPDLESWATGIAAVLEPGGRFVLVDFHP
                     AAEMLDAHWNHARAYPAGGEPLRLEEGVGDYVGESGGGLSPAGFSEGVQGFENPASCH
                     LFRWGLGEIVTALAASGLRISVLEEYPYANGERHFSGMRELPGRRMVPPEGVPDVPLM
                     YGVRAERTEG"
     gene            2895..3287
                     /locus_tag="VNT12_07040"
     CDS             2895..3287
                     /locus_tag="VNT12_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI45911.1"
                     /translation="MATAVRALGEVALRVNDLDKSQAFYAEVIGLELMRRFEHAAFFR
                     VAEGYAGHTTILALFDRKTSVEQAHTTVDHLAFTIALSDYEAEKRWLEELGLSVTTAT
                     HDWVHWRSLYLEDPDGNEVELVCYDPKA"
     gene            complement(3827..4168)
                     /locus_tag="VNT12_07045"
     CDS             complement(3827..4168)
                     /locus_tag="VNT12_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45912.1"
                     /translation="MQARPDKGEPTLACDFTVMDTEQRERYRALRPQLSKDLHEVREL
                     EDGYAFRHSSKAEVLIGLAEYVTLERLCCPFFDFAIEVGRDGGEVWLKMTGGQEVKRI
                     LQAAQRYDQDS"
     gene            4563..5513
                     /locus_tag="VNT12_07050"
     CDS             4563..5513
                     /locus_tag="VNT12_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HWI45913.1"
                     /translation="MSDGMQPLYDRDSAHVFDEHRALLNSVAYRILGSVTDAEDTVQE
                     AWLRWSGVNPSDVIDSRAFLVRVTTRLAIDRLRRAKTRRESYVGSWLPEPILTGRDVA
                     EDVALAESVSMAMLVVLETLSPLERAVFVLREAFGMPYAEIADILGRKEDAVRQLGRR
                     ARDHVQERRSRFDTDESEQRRVTERFLEATSTGNLDALMAVLSPEVTLVADGGGRALA
                     PRRPVRGAEKVARFLLAVATEERMARFLRSVGSEPIVADLRVRVALVNGGPGILVVSG
                     DKPISALVLDVSEGVVQTIHLVANPEKLAGIRGSATPRRS"
     gene            5620..6948
                     /locus_tag="VNT12_07055"
     CDS             5620..6948
                     /locus_tag="VNT12_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI45914.1"
                     /translation="METRFSQTDVVVVGGGMAGLTAATYLAREQIDVTVIEKASYLGG
                     RAATQDFDGFRFNRGGHALYTGGAASRVLMELGVSYDYGIPKQTFVMQGGKLSPFPVD
                     PLGLLRADLLNAGDKLALVGLLVALGAAKPQALANTSVQGWLDQNVRRPQLRRLMMGL
                     ARTFVYSTALDLVSAELFVEKFQRALRHPVHYVDGGWRTLVDGLRAAAERAGARIVSN
                     VQVESVEIDNGHARSVRLRDGSVVRASAVVVATSPRDAAKLVDGGDHPAMRQIVEGLI
                     PARIACLDVALNRLPVPGRPIVQDLDGPRFMSAQSVYSRVAPEGAALIISFKQLDPRH
                     PGDPREDERDLEDLLDAAQPGWRDALIKRQYLPRIEAVGTLPTAREGGFAGRPDPRVT
                     GLDNLYLAADWVGPEGFLVDASMASARRAAELVLEDGWLSRVKVAAGSVG"
     gene            complement(7055..7255)
                     /locus_tag="VNT12_07060"
     CDS             complement(7055..7255)
                     /locus_tag="VNT12_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HWI45915.1"
                     /translation="MPLVELVAIGRKSGKERSTMLAVPIVDSDRLVLVASFGGDDRHP
                     AWYLNLQLTQRYAPRLQARLAP"
     gene            7266..7610
                     /locus_tag="VNT12_07065"
     CDS             7266..7610
                     /locus_tag="VNT12_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWI45916.1"
                     /translation="MPKIRPFDEPLLAEVFGFIEDRYREHISLRDVARAVSLSPGHLT
                     TVVRRKTGRTVQEWIAERRVAEARRLLVQTDLAVEEIGRSVGYIDAGYFVRSFRHAHG
                     MTPLGWRRASRP"
     gene            complement(8102..8782)
                     /locus_tag="VNT12_07070"
     CDS             complement(8102..8782)
                     /locus_tag="VNT12_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI45917.1"
                     /translation="MEDQLTTTSYAVLAQVAVRPWSTYELVQQRVRYFRYVWPRAESA
                     IYREAKRLSSMGLIEGKKEYTGKRARTVYSITEEGKEALREWLGTPISPFSMNFEAIL
                     RIFAAPLGTKEEIIHTVEQVRADAQEMLQFAGEVKQEFIDGINVTQDQVYIRALPVDF
                     FISLLNTVEAWAERTIAEIETWDDIMPSDEKNARGLEKIRQLPVDTPEEALKDVSIPP
                     KSQTRSRS"
     gene            8880..>9290
                     /locus_tag="VNT12_07075"
     CDS             8880..>9290
                     /locus_tag="VNT12_07075"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI45918.1"
                     /translation="MTDRPRSEYIETLVIGGGQSGLVMGYHLSRRGLPHAIVDANDRI
                     GDAWRNRWDSLRLFTPNRLNGLPGMPFPGYRWGFPSKNEMADYLESYARRFDIRVETG
                     VRVERLSREGDLFVSTAGNRRFEADNVVVAMSSWQ"
BASE COUNT         1764 a         2787 c         2909 g         1830 t
ORIGIN      
        1 tgtgttagct ctgcatggat atccgctgtg gtgaatggga gggggagctc tacacacacg
       61 gggaggtgga actctcttgt ggggggactt cgcctcctcg attgtgcgta ccctctagag
      121 cttgccacaa ctggtttgca aaggaagcgg cgaggcgtaa tcaacggaca cttgacccta
      181 gtttgaccct aacccggacg caataccctg caataccggg caactgaggg aacagaagat
      241 cgcttactta tgcgggatat gcaagcgtgt gcaaccctca gcaaccactg accgcacact
      301 cgtagctgga gcaggggtaa gcggttcgag tccgctcgtc ggctctctcg aatcggctag
      361 gacgacagct cttcaggaac ggggagtcgg caccgttcta ggtggcatta tctgaggtgg
      421 ccatcatcgt gaggaggtcg acccccgaag catggagccg ctgaagttgg aagagtcgac
      481 cgcctcgtgg gccatgacac atccgctcaa gctctagggc ccgttcactt ctcgacggtg
      541 accacgacct tgccacgtgc gtggccttgc tcgaggtagc gaatggcctg aggggcctcc
      601 ttcagaggaa aggtcctgtc gatgacgggt gtgagcttgc cggcttctat caaatctttg
      661 agggtcaaga gatcttgctg ccgttgctta tacatgaggc cgagcagctt ctgctccgag
      721 aagcgcgagc gcaggggggc aaagatctgg cgttggaagc caccgagcca gcggtctccg
      781 ccttcgccac cgacgatgac gagggtgccc tgcggcgtga gggcccggcg cagttgcgac
      841 aacggccgcc ggcccgcggt gtcgaggatc aggtcgaatc ggcgcgtgcc gtcggtgaag
      901 tcctctcggg tgtagtcgat gacctcgtcg ccgccgatgg cccatactaa ctccgctttc
      961 gacctgctgc acactcccgt gaccaccgct ccgaacgctt ttgccagctg cactgcgtac
     1021 gtccccacac cgcccgccgc accgataata agcaccttct gttcgggctg gatcttgcct
     1081 gcgtcgcgca ggccgttgag ggcggtcatg cctgagaccg gcacggccgc ggcctgctcg
     1141 aagctgaggt tcgacggctt gggcgcgacg cgctccgcct tggcgcaggc gtactctgca
     1201 aattagccgt cgcacgttcc atacacctga tcaccctgct tgaaccgcgt aacgtttctc
     1261 ccggccgcct cgacgctacc ggcgaagtcg gtgccacgaa cgccccgtct tgggcttgcg
     1321 caagccgaag cccataacgc gaaccagata gggtaggcca gtcatcagat gccagacgcc
     1381 tagatcgacg cccgcggcat gcacgcggat gagcagatcg tcgtccctgg gttgcggttt
     1441 ctcgacctcg ccaagctcga gcacgttggc cgagccgtag ctgtcttgaa cgatggcttt
     1501 catgactgct atgaccctcg gtcgacggaa gacggttctt tcgcccttgt tccatgcccg
     1561 caatcgcagc gcggaccact gataatacgg agcgttaccg tgtgcaagga gactctgcgt
     1621 aattaccccg acgacgcagc actcaacagc gaggtagtat ccagccgtgg aacgtagtct
     1681 cagagagcag aaccgccgct cgtggaacgc cgtggtcggg gcacacgaga gccaccgggg
     1741 gaacctcgcc gctttcctgc gcgcgggcgg taccaccctg tttccggagg aacgtgagtt
     1801 gctcggtaac ctcgccggcc agactgtggc gcacctgcag tgcaactggg ggggtgacag
     1861 cctgagcctg gccacgctcg gtgcatcggt ggttggggtg gatctcagcg accaggccgt
     1921 gagatctgcc cgccagctct ccgagagagc cgggatcccc gcgtgtttcg agcacgcgga
     1981 cgtctatgac tggctcgcag ccgccgcgcg ccgcgggcgt cgtttcgatg tcgtctattc
     2041 ctcctacggc gtcatctgct ggctaccgga tctggaatcc tgggcaacgg gcattgccgc
     2101 cgtcctggaa ccaggcggtc gcttcgtgct cgtggacttc caccccgcag cggagatgct
     2161 cgacgcgcac tggaaccacg cccgcgccta ccctgccggc ggggagccgc tgcgcctcga
     2221 agagggtgtc ggggattacg taggagagtc tgggggcggc ctctcgcctg cgggcttctc
     2281 cgagggggtg cagggtttcg agaacccggc ttcgtgccac ttgtttcgtt gggggctcgg
     2341 ggaaatagta acggcgctgg ccgcatcggg gctcaggatc tccgtcctcg aagagtaccc
     2401 ttacgccaac ggcgagcggc acttctcggg catgcgcgag ctgcctggta ggcgcatggt
     2461 acctcctgag ggagtacccg atgtaccgct gatgtacggc gtccgggccg aaaggaccga
     2521 gggctgacgg ccggtaccta catacggcga aaagggaggc gagcgtggcg atgacataga
     2581 cgccgcagag catcaggcct tagagccagt tggactcgcc atcgaggacg atagtcagcg
     2641 gtttgagtcc gctcgtcggc tctccagatt cggtctatta gtctgaatac atgaagcacg
     2701 ggaggctcct gtcactcatc catgagcata ttgacactac tgacgccgag gagggatgct
     2761 cgtctagtag cgagcaaaga ttgtcatatc ccgaggctac cttgcgttgc gttacagcag
     2821 gttgcgatga cgctgcgccg ggtagcatag tagcgtgaga gcatattcga tggacctcag
     2881 aacggaggcg attaatggcg acggctgtgc gggctctagg tgaggtggca ttgcgtgtca
     2941 acgatctcga taagtctcaa gctttctatg cggaagtgat cggtcttgaa ctcatgaggc
     3001 gctttgagca tgctgccttt ttccgggttg cggagggcta cgccgggcac acaactatac
     3061 tagctctctt cgaccgcaag acctccgtcg agcaggcgca taccacggtc gaccatctgg
     3121 cgttcaccat cgcactttcg gactacgagg cggagaagcg gtggctggag gagcttgggc
     3181 tcagcgtgac gacggccact cacgactggg tgcactggag atccctctac ctagaagatc
     3241 ccgacggcaa cgaagtcgag cttgtctgct acgatccgaa ggcgtgacgg ccgtgccgcc
     3301 ccggagagac ccaggagagc cgaacatccg aacgtttatc tactaaagac atgtatgctt
     3361 gtatccgcga aagcggagcc ggatttttcg gtggaggcgg gcgagggaat cccccaaata
     3421 cttttcgcaa cccaccccgg ctcacaattc agcgaattga ctccccgcgg acgccagtcg
     3481 cagctagcgc gttcccatcc ctactcgtgt attgagagca agggaactgg cgacgtgtaa
     3541 tacacagact cgtggtacca ctctgacacc accgggaagc aatactgtgc aacacggagc
     3601 aaagcagaga agaggaaacc ctttagatat gggccgtttg caagcctgtg caaacccctg
     3661 caacgcctga gtgatcactc gtatgagcag gaaagcggtc cgggtccgct cgtctgctct
     3721 ctcaattcgg cttggataag cactatatgc agaataggaa attcccggtg cgcagcgagt
     3781 ggcctattga caccactccg atgtgcttaa gagtgcattt caaatactag ctgtcctgat
     3841 cgtacctttg tgcggcctgg aggatgcgct tgacctcctg tccgcccgtc atcttgagcc
     3901 acacttcgcc cccgtcgcgt ccgacctcga tggcgaagtc gaagaacggg cagcacagcc
     3961 gctccaaagt gacgtactcc gccagcccga tcagtacctc ggccttcgac gagtgcctga
     4021 aagcgtaacc gtcttccagt tcccgcacct cgtggaggtc cttgctcaac tgaggccgca
     4081 acgcccgata ccgctcgcgc tgctcggtgt ccatcaccgt gaagtcgcag gctagggtcg
     4141 gttcgccctt gtcgggtctc gcttgcatct gcttctcctt ccttgtccta ccccctcttt
     4201 acgaacaacg acaactatca gtcagcccac cgctcttcct acattcctag ctcagccgca
     4261 cggacgcggg aatccggcga attaggtggc ttgaaagacc ccaggaacca gtaggtcggt
     4321 ggcctggtgg agtactgcca gtagtagtta ggggtgtctt gcacgacctc gggataaagg
     4381 atctcgtccg atgacgagcc acgacggttg gtgcgaaatc acggcaacac aggggactgc
     4441 gcatcccagg tagaaacacc accacaatat cagcgaccca gaggttacca tgtcacgttt
     4501 cagacgatca atgcgtcttc taggcagcgg atacgttcga gtctgcgcgg ttaggaggta
     4561 gggtgtccga cggaatgcag cccctgtacg atcgggactc agcccacgtc ttcgacgagc
     4621 accgcgctct gctgaactcg gtagcttacc gcatcttggg cagcgtgacc gacgccgagg
     4681 acaccgtgca agaagcctgg ctgcggtggt ctggcgtgaa cccttcggac gttattgatt
     4741 ctcgcgcctt cctcgtgcgt gtgaccactc ggctggccat agatcggcta cgccgcgcga
     4801 agactcggcg cgagtcctac gtcgggtcct ggctgcccga accgatcctc accggccgcg
     4861 atgtcgcaga ggacgtggcg ctggctgagt ctgtatctat ggcgatgctc gtggtgctgg
     4921 agacgctctc gccgctagag cgggcagtat ttgtgttgcg ggaggccttc gggatgccgt
     4981 acgccgagat cgccgatatc ctcggccgca aggaggacgc ggtccgacag ctcggtcgcc
     5041 gcgcccgcga ccacgtccag gaacgcagat cccgcttcga caccgatgag tccgagcagc
     5101 gccgagtgac cgagcgcttc ctcgaagcaa cctccaccgg aaacctggac gcgctaatgg
     5161 cggtcctctc gcccgaagta acgctcgtgg ccgatggcgg tggacgtgcg ctcgcgcctc
     5221 gtcgccccgt ccggggggca gagaaggtcg cccgcttcct gctcgccgtc gcgaccgagg
     5281 agcggatggc ccggttcctg aggtccgttg gaagcgagcc gatcgtcgcc gacctgcgcg
     5341 tccgtgttgc gctggtaaat ggcgggccgg gcattctggt cgtctccggg gataagccca
     5401 tctcggcgct tgtcctcgac gtgtccgagg gggttgtcca gaccatccat ctcgtggcca
     5461 accccgagaa gctggccggc atccgcggaa gcgcgacgcc gcgacgctcg tgacccagcg
     5521 gaagaacata ccagcttctg ccacatgtca cgcgcgcaag gggctgtttc gtctagaagg
     5581 taagagcagc taacgacggt cgggaaggag caccataaag tggagacaag attctcgcag
     5641 acggacgttg tcgtggtcgg aggcgggatg gccggcctga ccgcagccac ctacctggca
     5701 cgtgagcaaa tagacgtgac cgttatcgag aaggcgtctt acctgggtgg tcgcgcggca
     5761 acccaagatt tcgacggctt ccggttcaac cgcggcgggc atgctctcta caccggcggt
     5821 gcggcctcgc gagtactgat ggagctcggc gtctcatacg actacggcat cccgaaacag
     5881 accttcgtaa tgcagggagg caagctgagc cccttcccag tggatccgct cgggttactg
     5941 cgtgccgacc tgctgaatgc gggagacaag ctggcgctcg tcggcctgct cgtggcgctc
     6001 ggcgctgcca aaccgcaagc cctggcgaac acaagcgtgc aaggatggct ggaccaaaat
     6061 gtccgtcgcc cacagctgcg ccggctgatg atgggactcg cgcgcacctt cgtctacagc
     6121 acagcgctgg acctcgtcag tgcagagcta ttcgtcgaga agtttcaacg ggcgctcagg
     6181 caccctgtcc actacgtgga cggcggatgg cgcacgctgg tggacgggtt gcgcgccgcg
     6241 gcggagcggg ccggggcacg tatcgttagc aatgtccaag tcgagagtgt cgagatcgac
     6301 aacggccacg cgcgcagcgt ccgcctgcgc gatggtagcg tcgtgcgagc ctcagcagtc
     6361 gttgtcgcga ccagcccacg ggacgccgcg aagcttgtgg atgggggaga ccacccggcc
     6421 atgcgccaga tcgtggaggg cctgatcccg gcacgcatcg cctgcctcga cgttgccttg
     6481 aaccgtctgc cggttccggg ccgtccgata gtacaagacc tggacggtcc gcggttcatg
     6541 agcgcccagt ccgtctactc gcgcgtcgct cccgaagggg cagccttgat tatctccttc
     6601 aagcagctcg atccgcggca tccaggggac ccgcgcgagg acgagcgtga tctcgaagac
     6661 ctgctcgacg cggcgcagcc tggatggcgt gacgcgctga tcaagcgaca atacctaccg
     6721 cgtatcgagg ccgttggcac gctcccaacc gccagagaag gcggcttcgc cggtcgaccg
     6781 gatccccggg tgacgggctt ggacaacctc tacttggccg cagactgggt cggcccggag
     6841 ggcttcctgg tcgacgccag tatggccagc gcccgacgtg cggcggaact ggtattggag
     6901 gacggctggc tctcgcgggt aaaggtggct gccggttcgg ttggctagtt cgatgtgcgg
     6961 acttttaaga tgaatagcgt cggcgtagcc ctcgaaaacc gaggtgatcc ggggccagag
     7021 ttcggcccgc tcctcttctg tgcgatgcgg gcgatcatgg tgcgagtcga gcctgcaaac
     7081 gtggcgcgta cctctgggtt aactggaggt tgaggtacca cgccgggtgc cggtcgtcgc
     7141 cgccgaacga ggccaccagc accaagcggt cgctgtcgac gattgggacc gcgagcatag
     7201 tcgagcgttc cttgcccgac ttgcgtccga tggcgacgag ttcgacgagg ggcatcccga
     7261 aggctttgcc aaagatccgc cccttcgacg agcccctgct ggccgaggtc ttcggcttca
     7321 tagaggatcg ctaccgcgag cacatctcgc ttagggacgt ggcgcgggcg gtgagcctgt
     7381 cgccgggtca tctcacgacg gtcgtaaggc gcaagacggg acggacagtg caggagtgga
     7441 tcgctgagcg ccgtgtggca gaggcacgca ggttgctcgt acagacggat ctggcggtgg
     7501 aggagattgg ccgaagtgtc ggctacatcg atgcgggcta cttcgtaagg tccttcaggc
     7561 acgcccacgg catgaccccg ctcggctggc gccgcgccag ccgcccataa gccatgaaaa
     7621 tggagccgcc gccaaggcta ttttcacacc agtgttccaa agcccttgcc gggccacccc
     7681 gaagccatga aaatgaactg tagccaaggc tattttcacg ccagtgccta gaaggtgaat
     7741 tctccgaagt ctatatccag cagtctgcaa ggagcgcctg gtaggatcga ctgaggagcc
     7801 ctttactccg ctcctatgca tagaaattaa cgctatatgt gctactcgtg cataattatc
     7861 cctcctggta aatgggatgg gaaggcctcc gcagaggtgg aaatctcacg tcgaaacttc
     7921 gcctcatcga ttcggcggac cctctagctc ttgccacctc tcaattgcaa aagggccgac
     7981 gaggggtaat ccacggactc tcctgatacc acttgaccct aatttgaccc taacccggat
     8041 gcaatgccct gcaacagtgg gcaaggcagg gaacaggaaa cttgatgtcg acaaccaacg
     8101 gtcaggagcg ggaccgcgtt tggctctttg ggggtatgga tacatctttg agtgcctctt
     8161 ctggggtgtc gacgggaagc tggcgaatct tttcgaggcc ccttgcgttc ttctcgtctg
     8221 agggcatgat gtcgtcccag gtctcgattt ctgcgatggt gcgttcggcc catgcctcga
     8281 cggtgttgag cagactgatg aagaagtcga ccggcagggc tctgatgtag acctggtctt
     8341 gggtgacgtt gatgccgtcg atgaactctt gtttgacttc gccagcgaat tggagcatct
     8401 cttgggcatc ggcgcggacc tgttcgaccg tatggatgat ctcctctttg gttcccaggg
     8461 gagcggcgaa gatgcgcagt atcgcctcga agttcatcga gaacggagag atcggagttc
     8521 ccagccactc ccgtaacgct tctttgcctt cttcggtgat cgagtagacg gttcgggctc
     8581 tctttccggt gtattccttt ttgccctcta tcagtcccat cgaggagagc cgcttcgctt
     8641 cccggtagat ggcgctctcg gcccggggcc agacgtatcg gaagtagcga actcgttgct
     8701 ggaccagctc gtaggtggac cagggtcgca ctgctacctg ggccagtact gcatacgagg
     8761 tggtcgtcag ttgatcttcc atccctacct ctctcttgac atactccaaa ttagagtata
     8821 ctatacccca aaatactcca aattggagta taggggatcg ggtccgagga ggtaacgaca
     8881 tgactgatcg acctagaagc gagtacatcg agactctcgt gatcggaggt gggcagtccg
     8941 ggctcgtcat ggggtatcac ctctcacggc gcggcctccc tcatgcgatc gtcgatgcca
     9001 atgaccggat cggtgacgct tggcgtaacc ggtgggactc gctgcggctg ttcaccccca
     9061 atcggctcaa cgggctaccc gggatgccgt ttcccggata ccgctggggg ttcccgagca
     9121 agaacgagat ggccgactat ttggagtctt acgcccggcg cttcgatatt cgcgtcgaga
     9181 ccggtgtaag ggtggagaga ctctctcgag agggcgacct cttcgtgtcg actgccggaa
     9241 accgacggtt tgaggccgac aacgtggtgg ttgcgatgtc gagctggcaa
//
LOCUS       DATMJA010000296         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_217175, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000296 DATMJA010000000
VERSION     DATMJA010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217175"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VNT12_07080"
     CDS             <1..553
                     /locus_tag="VNT12_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45919.1"
                     /translation="SLYKIGDAYFVQDGNHRVSVARQRGVEMIDAEVIELRSRVPVDS
                     ALTARDLLHKLEHRRLLERLPIDRVLPEVEVEFSDVADYRRLATYMEAHGFRVSQLWK
                     RYVSSEEVLRDWYEYDYCPIAEMIREEHILDAFPDRTELDLYLWIVYHRERLALEARD
                     EKISPQAAKDDILKNAPRRRRRP"
     gene            707..1744
                     /gene="sppA"
                     /locus_tag="VNT12_07085"
     CDS             707..1744
                     /gene="sppA"
                     /locus_tag="VNT12_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWI45920.1"
                     /translation="MDTFADGPSRQPERPAGEPRRRRRWPFVVGGLMALGLIGLVIVA
                     VVLILAAGGPTAPPAYVEEYVAGDGPYKIAAVPVEGEIASADDTLGGTQPTATPEGLA
                     DALRQAGQDTSVVAVVIEVNSPGGGVTASDEMHQSILNFKKNTGKPVVVSMGDTAASG
                     GYYISTAADRIVANETTLTGSLGVIIPLNNFAEAAEKYGIKQVVIKSGKYKDMGSAFR
                     EMRPDEREIFQSIVDQEYAEFVDVISEGRGIPEDRVREIADGRVYSGIQARDLGLVDS
                     FGSLDEAAATAGRLAGAKDTTVVRYVQEPTLTETLLARLAPQEPQAQQIMEAAGLDLE
                     PKPYYLYLPGA"
     gene            complement(1835..>2034)
                     /locus_tag="VNT12_07090"
     CDS             complement(1835..>2034)
                     /locus_tag="VNT12_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45921.1"
                     /translation="HNRWAKDIYLRARDRGCDHAHAIRILGRAWVRVIFRMWRDGLPY
                     DPARHGGLQRLRSAEELPQGV"
BASE COUNT          407 a          654 c          626 g          347 t
ORIGIN      
        1 gagcctgtac aagatcgggg acgcctactt cgtccaggac ggcaaccaca gggtctcggt
       61 tgcccgccag cggggggtcg agatgatcga cgctgaggtc atagagctcc gcagcagggt
      121 gccagtggac tcggcgctaa cggcccgcga cctgctccac aagctcgagc accgccgcct
      181 tctggagcgg ctgcccatcg accgcgtgct acccgaggta gaggtcgagt tctccgatgt
      241 cgctgactac aggaggctcg caacctacat ggaggcgcat ggcttcaggg tctcccagct
      301 ctggaagcgc tacgtctcat ctgaagaggt ccttagggac tggtacgaat atgattactg
      361 cccgatcgca gagatgatca gggaggaaca tatcctcgac gccttccccg accgcaccga
      421 gctcgacctt tacctctgga tcgtctacca ccgcgagcgt ctggcgctgg aggcccgcga
      481 cgagaagatc tcaccccagg ccgccaagga cgacatcttg aagaacgccc ctcgccggcg
      541 acgccgaccc tgaaccagga cctaccgccg agaactatcg agctacgccg gctcgtgcgc
      601 cggctcaacc ggccttataa ttttcgctgt cggggtccgc ctcgctggtc aggcccgcga
      661 cctcaacgca aagatagggc gagcacacag cagtgaaggg agcattatgg acactttcgc
      721 cgacgggcct tcccggcaac ccgagaggcc tgcgggagag cccaggagac ggcgcaggtg
      781 gccctttgta gtcgggggtc tcatggcact cggtctgatc ggcctcgtca tcgtggcggt
      841 agtcctgatc ctggcggctg gcggcccgac ggcccccccc gcctacgttg aagagtatgt
      901 ggccggcgac ggaccgtaca agatagccgc cgtgcctgtt gagggtgaga tcgcctcggc
      961 tgatgacacc ctgggcggga cgcagccgac ggccacgccg gaaggactcg cagacgccct
     1021 gcgccaggca gggcaggaca cgagcgtcgt cgccgtggtg atagaggtca actctcctgg
     1081 tggcggggtc acggcgagcg acgagatgca ccagagcatc ctgaatttca agaagaacac
     1141 gggcaagccg gtcgtcgtct ccatgggaga tactgctgcc tcgggcggct actacatctc
     1201 gaccgcggcc gaccgcatcg tcgccaatga gacaaccctg acaggctcgt taggcgtcat
     1261 aatcccgctc aacaacttcg cagaggccgc cgagaagtac ggcataaagc aggtcgtcat
     1321 aaagagcggc aagtacaagg atatgggtag cgccttccga gagatgaggc cggacgagcg
     1381 tgagatcttc cagtcgatcg tggaccaaga atacgccgag ttcgtcgacg tgatctccga
     1441 gggccgcggc atcccagagg atagggtgcg tgagatcgcc gacggccgcg tgtactcagg
     1501 catccaggct agggatcttg ggctcgttga ctccttcggg agcctcgatg aagcggccgc
     1561 caccgcgggc aggcttgccg gcgctaaaga tacgaccgtg gtccgatacg tgcaggagcc
     1621 aaccctgacc gagaccctgc tggcacgcct agccccgcag gagcctcaag ctcagcagat
     1681 catggaggcc gcagggctag atttggaacc gaagccttac tacctctact tgcccggagc
     1741 ctgaccccta cggcgtcgtg cataaggctc tcgagagcct tatgcacgac gagcatttca
     1801 gcaagtcagc acgctccggc ttcgcctccg cttgtcagac gccctgtggc aactcctccg
     1861 ccgatcgcag acgctgcaaa cctccgtggc gagctggatc gtagggtagc ccatcgcgcc
     1921 acatcctgaa gattaccctc acccacgccc tccccaagat gcggatagcg tgcgcatgat
     1981 cgcaacctct gtccctcgcc cttaggtaga tgtccttagc ccatctgttg tgat
//
LOCUS       DATMJA010000297         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_868509, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000297 DATMJA010000000
VERSION     DATMJA010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868509"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VNT12_07095"
     CDS             complement(<1..525)
                     /locus_tag="VNT12_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWI45922.1"
                     /translation="MSVQIGLDVGTGGARVVAVDESGEIVAEASSEYPLHSPRPGWTE
                     QDPEDWWEGAKETIKKVATETGEQIAGIGLTGQMHGSVFLDSSGEVVRPALLWNDQRT
                     HRQCEEITSAVGEERLISIAGNPALTGFQAPKIIWLREEEPDNFGRVSHVLLPKDYVR
                     LQLTGEYATDASDAA"
     gene            complement(522..1601)
                     /locus_tag="VNT12_07100"
     CDS             complement(522..1601)
                     /locus_tag="VNT12_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWI45923.1"
                     /translation="MIKNSVGIWAFGPAVTRFVPPGYHNEVADESMVEKTERVAGGLH
                     DLLEGLEYHYPGEINEENVEEVLRVLAAHGMDLPVIAAGLHPDPTYALGAFINPDKGL
                     RRKGIDTLRRGIDLCARVGANFIIWPGAEGYNYTFQRPYAEIWRRFVEGVAELTDHAN
                     EKGVKVFLEHKNSEPAMKILMQNIGMTLFTIQKVNTLGVDTTNLQVNMDWQHLIMNGE
                     NLAEYADLLASEHKLGHQHGNDGWGTFDDDNVVGTNFFMQTLELAQTLQDTGYGEDGE
                     IMGFDLYPYTEDQVAAVRRAILQWEFVWDLARKIDRTGLREAKTRADALAGQKAVYAA
                     LGMDEGYEAEISSRRKEAREGRGEA"
     gene            1781..2551
                     /locus_tag="VNT12_07105"
     CDS             1781..2551
                     /locus_tag="VNT12_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45924.1"
                     /translation="MDLGLENKSFVVGGASSGLGRAVAEQLVAEGARVLLVARSEDAI
                     EEVARELGEQAEQCMADVSQSSGVDKVATVAIDRFGTLDGVLVNAGGPPFGPALELTD
                     EQWLDAFRLLLGGPVRLLRTLVPQMNDGASVLFITSSSVREPIQGLDTSNVLRPGVAA
                     LVKCLAREVGPRVRVNSMAPGRIDTARSRSLDEARAQDFGISTEEQRRNVSEGIPLGR
                     YGEPAELGRAAAFLLSPAASYITGVSLQVDGGLVSAVP"
     gene            2681..>3544
                     /locus_tag="VNT12_07110"
     CDS             2681..>3544
                     /locus_tag="VNT12_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI45925.1"
                     /translation="MVFELSKKTRGFRERVEAFMDELVYPNEAKYHEQLEASGSRWDV
                     PQVMEEIKAEAKARGLWNLFLPDSELGAGLTNLEYAPLCEVMGRSPIAPEAFNCNAPD
                     TGNMEVLVRYGTEAQKERWLKPLLDGEIRSCFAMTEPDVASSDATNIRARIEREGDEY
                     VVNGRKWWISGAGDPRCRISIFMGKTDPDAPRHEQQSMILVPLDTPGVEVERTLPIFG
                     YDDAPHGHAEISFHDVRVPLENIIWDEGKGFAIAQGRLGPGRIHHCMRLIGVAERALE
                     LMCERVKGRETF"
BASE COUNT          606 a         1103 c         1130 g          705 t
ORIGIN      
        1 cgccgcgtcc gaggcgtccg tggcgtactc gcccgtgagc tggagcctca cgtagtcctt
       61 gggcaggagc acgtgggaga cccgcccgaa gttatccggc tcttcttctc ttagccagat
      121 gatcttcggt gcctgaaagc ctgtgagggc cgggttgccg gcgatggaga tcagacgctc
      181 ctcccccaca gcactagtta tctcttcgca ctgcctgtgg gtgcgctggt cgttccataa
      241 cagcgccggc cttactacct cacctgatga atcgaggaac acggagccgt gcatctgccc
      301 tgtgagcccg atgcctgcta tctgctctcc tgtctctgtg gcgactttct ttatggtctc
      361 tttggctcct tcccaccagt cttcgggatc ttgctcggtc cagccagggc gcgggctgtg
      421 gagcgggtac tctgaggagg cttcggcgac tatttctccc gactcgtcca cggcgacgac
      481 cctcgcgccg ccggtcccga cgtcgaggcc gatttgaaca ctcacgcctc tcccctgccc
      541 tcacgcgcct ctttgcggcg gctggagatc tccgcctcat aaccttcgtc catgccgagc
      601 gctgcgtaga ccgccttctg gccggccagg gcgtcagcac gggtcttggc ctcccgaagc
      661 ccggttctgt ctatcttccg cgccaggtcc cagacgaact cccactgcag gatggcccgc
      721 ctgacggcgg ccacctggtc ttccgtatag ggatagaggt cgaagcccat gatctcccca
      781 tcttccccgt agcccgtgtc ctggagcgtc tgggccagct cgagggtctg catgaagaag
      841 ttggtgccga ccacgttgtc gtcgtcgaag gtgccccatc cgtcgttgcc gtgctggtgg
      901 ccgagcttgt gctccgaggc caagaggtct gcgtactcgg ccaggttctc gccgttcatt
      961 atgaggtgct gccagtccat gttgacctgc agattggtcg tgtccacccc gagggtgttg
     1021 accttctgga tcgtgaagag cgtcataccg atgttctgca tcaggatctt catagcgggc
     1081 tctgagttct tgtgctccag aaagaccttc acgcccttct cgttggcgtg gtcggtcaac
     1141 tcggcgacac cctcgacaaa gcgacgccag atctccgcgt aaggacgctg gaaagtgtaa
     1201 ttgtagccct cggcgccagg ccagatgatg aagttcgctc ctactcgtgc gcagaggtct
     1261 atgccgcgcc tcagggtatc tatgcccttg cgacggagtc ccttgtcggg gtttatgaac
     1321 gcgcccagtg cgtaggtcgg gtcggggtgg aggccagcgg cgatcacggg caggtccatc
     1381 ccgtgggcgg cgaggacacg cagcacctcc tcgacgttct cttcattgat ctcaccagga
     1441 tagtggtact cgagcccttc caggaggtcg tggagcccgc cggcgacgcg ctccgtcttc
     1501 tcgaccatag actcgtccgc gacctcgttg tggtagcccg gcggcacgaa gcgggtcaca
     1561 gcagggccga atgcccatat cccaacagag ttcttgatca tctggctcct ccttgcctct
     1621 aatctacaag agacgcgctg atacccccgt accatcatag cccgaacctt gcaagcattt
     1681 cagtttccaa tcattacttt cagcccaccg ccctcatgca tcacgagagg gtgtaggatt
     1741 ggtcccggtt ggcgtactca aggcgaggag gcgaacgact gtggatctag gcctggagaa
     1801 caagtcattt gtggtaggag gcgcgagcag cgggctcggc agggcggtag cggagcagct
     1861 ggtcgcggag ggcgccaggg ttctcctggt cgcacgcagt gaggacgcca tcgaggaggt
     1921 ggcgcgagag ctcggcgagc aggccgagca gtgtatggct gacgtctccc aatcgtcggg
     1981 cgtagacaag gtcgccacgg ttgctatcga ccgcttcggt acgctcgacg gcgtgctcgt
     2041 caacgccggt ggcccgccct tcgggcctgc gctggagctg accgacgaac agtggctcga
     2101 tgccttccgt ctgctcctgg gcgggcccgt gcggttgctg cgcacactcg tcccgcagat
     2161 gaacgatggc gcctccgtgc tctttatcac ctcatcatcg gtgcgcgagc cgatacaggg
     2221 cctcgacacc tcgaacgtgc tgcgccccgg ggtggcggcc ctcgtcaagt gcctggcgag
     2281 agaggtcggg cccagggtca gggtcaacag catggcccca gggcgcatcg acaccgcccg
     2341 ctcccgctcg ctcgacgaag cgcgggccca ggacttcggc atctcgaccg aggagcagcg
     2401 ccgcaacgtg agcgagggaa tccccctcgg ccgctacggt gagcctgcgg agctaggaag
     2461 ggcggcagcg ttcttgctct cccccgccgc ctcttacatt accggcgtct ctctgcaggt
     2521 cgacggtggg ctcgtctctg ccgtgccttg atgcagaaag cttcaggttt cagggtttgc
     2581 acctctggtg ttctacggcc ccagggccct cgcccggatt gcgacaccca ggatgctgtg
     2641 ccagaatcgg atctccggct gttaggtgag aggagaggtt gtggtcttcg agctttccaa
     2701 gaaaaccagg gggtttcggg agcgggtcga ggcgttcatg gacgagctcg tctacccgaa
     2761 cgaggcgaag taccacgagc agttggaggc gtcggggagc cggtgggacg tgccccaggt
     2821 catggaggag atcaaggcgg aggcgaaggc gagagggctg tggaacctct ttctgcccga
     2881 ttcggaactc ggcgccggcc tcaccaacct ggagtacgca cctttgtgtg aggtcatggg
     2941 ccgtagcccg atagcccctg aggctttcaa ttgcaacgcc cccgacacgg gcaacatgga
     3001 ggtgctcgtt cgctacggca cagaggcgca gaaagagcgc tggctgaagc cgttgctcga
     3061 cggtgagata aggtcctgtt tcgcgatgac cgagccggat gtggcatcct cggacgcgac
     3121 caatatcagg gcgcgcatag agagggaagg ggacgagtac gtcgttaatg gcaggaagtg
     3181 gtggatctcc ggtgccggag acccgcggtg cagaatctcg atcttcatgg gcaagacgga
     3241 ccccgacgct ccgcgccacg agcagcagtc catgatcctt gttccgctcg atacgcctgg
     3301 cgtagaggtt gagcggaccc tccccatctt cggctacgac gacgccccac acgggcacgc
     3361 ggagatctcc ttccacgacg tgcgcgtgcc gctagagaac attatctggg atgagggcaa
     3421 aggcttcgca atcgcccagg gacgcctcgg accagggcgc atccaccatt gcatgaggct
     3481 cataggcgtg gccgagagag cactggaatt gatgtgcgag cgggtcaagg ggcgggagac
     3541 gttc
//
LOCUS       DATMJA010000298        10502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_158361, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000298 DATMJA010000000
VERSION     DATMJA010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158361"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VNT12_07115"
     CDS             <1..197
                     /locus_tag="VNT12_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45926.1"
                     /translation="TLRGTRKDWLTFLQPEPPPFYNDLLAMNSRIPSFSIEGDRAAFV
                     RHLRTLSKLFHLAQLMGSHA"
     gene            190..1044
                     /locus_tag="VNT12_07120"
     CDS             190..1044
                     /locus_tag="VNT12_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI45927.1"
                     /translation="MPEFDPITGRYAYLDVAGARNRVYFEEAGEGRPLLCLHTAGADS
                     RQWRHLLCDAEVTGRWRVLAFDMPYHGRSTPPVGWWEKEYLLTTETYARTVMAFVRAL
                     DLEQPVVLGCSMGGAVVLELASRHSAELSAVIGLEAASKIEDRFSDWTVIPDLDGSIV
                     AASWTYGLMAPQSPEAARREVWWTYAQGGPGTYRGDTFFYSVDWDFRGREEKIDTATC
                     PVHLLTGEYDYACTPEESAATAAAIPDAKFTKMRGIGHFPMAENYPRFREHLLPILRE
                     LEGLLDGP"
     gene            1077..1508
                     /locus_tag="VNT12_07125"
     CDS             1077..1508
                     /locus_tag="VNT12_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWI45928.1"
                     /translation="MATQEEKQAYIDEMVRKRGYVLDYHKVMAAHDYEVLQAANGLVN
                     AAYLKQRRLDRKTKELIFIASLTVMRASKGHIQSHIQVALDLGLSKEEILEAIEIALP
                     EAGVVAFQEGFEAWREAVGAEGIEPTVAAHQGGSGSEDNQD"
     gene            complement(1505..2302)
                     /locus_tag="VNT12_07130"
     CDS             complement(1505..2302)
                     /locus_tag="VNT12_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HWI45929.1"
                     /translation="MEQKLRTDSTRVQEVRARIRDGEHTGPTSGLAHGFVHANLVLLP
                     EEFAFDFLKFCVRNPKPCPILEVTEAGSPEPVTTAPGADLRTDVPRYRVYEDGELMEE
                     PQDILRHWRDDLVGFLIGCSFTFEAALLGAGLRVAHLDQGRNVPMYITDRQCVPSGPF
                     SGPLVVSMRPYEPEELPLVVSVTGRYPSMHGAPLHVGDPEALGIRDLAKPEFGEPVGI
                     ERGQMPVFWACGVTPQAVAMKARPPLMVTHSPGHMFITERRHSEYEV"
     gene            complement(2377..3162)
                     /locus_tag="VNT12_07135"
     CDS             complement(2377..3162)
                     /locus_tag="VNT12_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HWI45930.1"
                     /translation="MSGGSPFQVYAIKYATREARSGEHFYGGDPHDAPMPMDYYVWAV
                     VSPERTIVVDAGFTAEVAARRGRKHLRCPTEGLEALGIDCARVPYVILTHLHYDHVGN
                     LDKFPEATFVVQEAEMAFWTGRYAGKEHFRNIVETDDVVYLVRENFEGRLRFVAGSEE
                     VVPGIEVHWAGGHSSGLQVVRVKTARGFVVLASDATHFYANIEEDRPYSIVSELAQMY
                     GAFDVVRSLADSPAHVVPGHDPLVMQRYPAAKKGLDEVAARIA"
     gene            complement(3321..4727)
                     /locus_tag="VNT12_07140"
     CDS             complement(3321..4727)
                     /locus_tag="VNT12_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI45931.1"
                     /translation="MGCTVTTWFSRPPHKVSSLDICYSTDVNSILAHFNFSGRSAREI
                     ASGIEAAVRTGDIEPGERLPSVRRLAVELEVSPATVASAYRDLRSRGIVTGRSRGGTT
                     VSLRPPLRVRSEAPVPVGTRDLATGNPDPRLLPDLEEALRLVAMRTRLYREPLVVAEF
                     AAAAHVRLEEIGLDGSEFTVVGGAMDGIERVLQAHLRPGDRVAVEDPGYSGVLDLLAA
                     MGFERHPVRVDNEGMIPDTLAEALRFGVSAVVLTPHAQNPTGAFITPARAADLRAVLD
                     SAPEVTVVEDDHLGLSADAPYGTLVDRRSRWAFVRSVSKTLGPDLRVALLTGDPVTVS
                     RVEGRMSLGAGWISGVLQRLVAALWNDPGVDALVTQAARTYSARRQALIAALRLRNIT
                     AFGASGLNVWIPVTQESSVVAGLLERGWAVRAGEAHRLTAPPAVRVTTATLEPEEAEA
                     FSEDMLAVLHPPHRTRIA"
     gene            4706..6178
                     /locus_tag="VNT12_07145"
     CDS             4706..6178
                     /locus_tag="VNT12_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWI45932.1"
                     /translation="MSLRYSPSVARLTPYAPGLSAQDVASEFGISPSEVVKLGSGENP
                     FGPSPAAARAISEDLGLLNRYPEWTSAELGRSKGMQDLIAFDMGGTTAKVGVIQDGRP
                     RLAREFYADRYVGGDDLGGYAIRSPVIDLAEIGAGGGSIAWLDPAGVLKVGPQSAGSQ
                     PGPACYGRGGGSPTVTDAHCALGTLPSDSSEGETPSLRPDLARAAIEAQIARPLGWTV
                     ERSAHAILEIATANMAEMVRLVTVRRGLDPRDFALLAYGGAGPLHAGDIAREVGVEKV
                     VIPPYPGMFSAIGTVMCEVKYDLVQSLLRKVDVLEPEGVREAFRRLEHRAQGLLADEA
                     VPVDPESVRYSRHLDLRHEGQLHELGIALRRDGIPSHAEIEKDFRNAYEDSYGYRLPG
                     SAVELVNVRLEVVFRLWKPGFFPKDAQRIRTLTERERAIAEADGSEVQWRVLARHELV
                     PGEEVYGPTLIEDEGSVVRVLAGQSARRGDEGVLIVEERR"
     gene            6213..7850
                     /locus_tag="VNT12_07150"
     CDS             6213..7850
                     /locus_tag="VNT12_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWI45933.1"
                     /translation="MTTLEVVRGALGATTEEMKSIVMRASFSPLLTLSGDLSCAVLDG
                     KGQVVTQGNDIPVHLGAMPFTAQGILSKIPAEDIAPGDVLITNDPYVGGTLLSDVTVM
                     SPVFDEEGLVGFVASRVHWPDVGGASAGSSKATDGAIKEGLRIPPAKLVEENELDDKF
                     VELLLANMRVPSERRGDLEAQLAGNRRGVWRIEELVSRYGGGTVREVFEESQRYSQIL
                     VERALREIPDGEYQFEERLDGDGFEEEDAPPELTIRVTVAKTGGEIHFDFDGSSPCSR
                     GPVNAPFAVTASSVYYVVLAVLGGHIPPNSGAYRPVRIMAPEGTLVNAAYPAPVVAGN
                     TETSNRIIDVLLGALAGAAPERVTAGSYGSAGVYTLGGWEPARRRSFVHYETVGGGMG
                     AFRGGEGLSGMRVHMGNTMNLPIETVESRLPVRIRRYELISDSGGHGRWRGGRGVRKV
                     VQALCDGIDFSVLGERARTPAPGICGGATGKPANFFIRTGEGKEIALRSKASSPRLMQ
                     GWELWLETAGGGGYGDDAGEAQGVKARSLPGVEKKKM"
     gene            7975..9498
                     /gene="tcuA"
                     /locus_tag="VNT12_07155"
     CDS             7975..9498
                     /gene="tcuA"
                     /locus_tag="VNT12_07155"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HWI45934.1"
                     /translation="MRGNDVVIVGAGNAAFCAAHAARERVERVVVLEKASREWAGGNT
                     YFTAGAFRTTFWNLEELRPLLDDVSEELLAKTEVPPYTEDDFLADMRRVTQGRCDEEL
                     TGILVAEAAEAVRWLHEKGIRWRLMHERQSYKVDGKRRFWGGLVLGTVGGGKGLVEQH
                     TTAASRTGIEIRYGCEVTGLLRDDSRAVVGVACEGPEGREEIEAGAVVLAGGGFESDP
                     QARAQYLGPNWDIAKVRGTPHNTGEVLRMALEAGAQPYGHWSGCHAIAWDAAAPPVGD
                     RELTNLFSKQSYPLGILVNAEGQRFVDEGADFRNYTYAKYGAEILSQPGVIAYQLFDS
                     KTESLLRQDEYTAPGVSRFEADTMRALAEEIGVAPEALEKTIEEFNAAVQPGEFDPTI
                     RDGKCTEGVEPPKSNWAQPLDSPPFYGFAVTCGITFTFGGVRIDEAARVLDRAGRPIK
                     GLHAAGELVGGLFYHNYPGGSGLTSGAVFGRRAGATAATHAGARTSGFRNLAQENSR"
     gene            9495..>10502
                     /locus_tag="VNT12_07160"
     CDS             9495..>10502
                     /locus_tag="VNT12_07160"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI45935.1"
                     /translation="MSVRDRKVGFVGLGNMGGAMVEAILADGYRVWVYDIDAAAASAL
                     EPLGAQEAPGPGALAAQADVILTSLPDARAVRAVYLGEEGLVSGARPGTVLVELSTID
                     PATIEELERVVSERGAVLLDVPVSGSPDEARRGELVLTAGGEREALAAVEPILNCFGR
                     TIQYVGPAGAAKIVKIVNNMMTMGNVLVAAEAFCVGVKAGIAPDLLFEVLSGSGGRSH
                     HFTKRFPNALAGDFKPGFSVALGEKDLGLALELSRSLDMPSPTTGLVRQVYRIAMSEG
                     LSSEDIVAVIKLFEKWSGVQARGAARREASGGDHEVEAVGEGPNLTTELFRRIEAKLN
                     ED"
BASE COUNT         1988 a         3191 c         3481 g         1842 t
ORIGIN      
        1 tgacgctacg cggcacgaga aaggactggc tgaccttctt gcagccggag cctcccccct
       61 tctacaatga cctgctcgcc atgaacagcc gtatcccgtc gttctccata gagggcgacc
      121 gtgcggcgtt cgtgagacac ctgcgcacct tgagtaagtt gttccacctc gcgcagctga
      181 tgggtagcca tgcctgaatt tgatccgatc acgggccggt acgcgtacct ggacgtcgca
      241 ggcgcccgca accgcgtcta ctttgaggag gctggggagg gccggccgct cctgtgcctc
      301 cataccgcag gggccgactc acgccagtgg cggcacctgc tctgcgacgc agaggtgacc
      361 ggtaggtggc gggttctcgc cttcgacatg ccttaccacg gacgatctac gccgcctgtg
      421 ggctggtggg agaaggaata cctgctcact accgaaacct acgcgcgtac ggtaatggcc
      481 tttgtgcggg ccctagacct ggagcaaccc gtggtgctcg gatgctccat gggtggtgcc
      541 gtcgtgctcg agctagcgag caggcactcc gcggagttga gcgccgtgat cggccttgaa
      601 gccgcgagca agatagaaga ccgcttctcg gactggacgg tgataccgga cctggacggc
      661 tcgatagtgg ctgcctcttg gacctacggc ctcatggctc cccagagccc ggaggcagcc
      721 cgccgggaag tgtggtggac ctatgcccag ggcggcccgg gcacctaccg cggcgacaca
      781 ttcttctata gcgtcgactg ggacttccgc ggacgggagg agaagataga caccgccacc
      841 tgcccggtcc acctcttgac cggggagtat gactatgcct gcacgccgga ggagagcgct
      901 gcaaccgcgg cagccatacc cgacgcgaag ttcacgaaga tgcgcggtat cggtcatttc
      961 ccgatggcgg agaactaccc gcggttcagg gagcacctgc tgccgatact ccgcgagcta
     1021 gaagggctgc tcgacggccc gtgacctttg cccaatacag gaaggagcga acacagatgg
     1081 cgacccagga agagaagcag gcgtacatag acgagatggt gaggaagcgg ggctacgtcc
     1141 tcgactacca caaggtcatg gccgcccatg actacgaggt cttgcaggcc gccaacggcc
     1201 tcgtgaacgc cgcctacctc aagcagcggc gcctcgaccg caagacgaaa gagcttatct
     1261 tcatagcgag cctcacggtg atgcgggcca gcaaggggca catccaaagc cacatccagg
     1321 tagctctgga cctcggcctc tccaaggagg agattctcga ggctatagag atcgcacttc
     1381 ccgaagcggg tgtggtcgcc ttccaggagg gtttcgaggc atggagggag gcggtgggtg
     1441 ccgagggcat cgagccaacc gtcgcggccc accagggtgg ctccggctca gaggataatc
     1501 aggactaaac ttcgtactcc gagtgccggc gctcggtgat aaacatgtgt ccggggctgt
     1561 gggtgaccat gagcgggggc ctggctttca tggccaccgc ttggggtgtc accccacagg
     1621 cccagaacac gggcatctgg ccccgttcga tcccgaccgg ctctccgaac tccggcttcg
     1681 cgaggtcccg aatccccaac gcttcaggat cccccacgtg tagcggggcc ccatgcatag
     1741 aggggtagcg ccccgtcacg cttacgacca gcggcagctc ctccggctcg tagggtcgca
     1801 tgctcacgac cagcggccct gagaagggcc ccgatgggac gcactgcctg tcggttatgt
     1861 acatggggac gttgcggccc tggtctaggt gcgctactct caggcccgcc ccgagcagcg
     1921 ccgcctcgaa cgtgaaactg cacccgatca agaaccccac gaggtcgtct cgccaatggc
     1981 gtagtatgtc ctgcggctcc tccattagct cgccgtcctc gtagacgcgg tacctgggta
     2041 cgtcggtccg caagtccgcg ccgggggccg tggtcacagg ctccggtgag cccgcctcgg
     2101 tgacctcgag gatcggacac ggcttcgggt tcctgacgca gaacttcagg aagtcgaagg
     2161 cgaactcctc tggcaggagg acgaggttcg catgcacaaa accatgcgcg agcccgctgg
     2221 ttggccccgt atgttcaccg tcgcgtatgc gggcgcgtac ctcttgtacc cgggtgctgt
     2281 ctgtccttag tttctgctcc atgaatcggt acctccttga ttcgcaagat acataaatct
     2341 tagcgcaatg aaaacagcat tttcgacggg caaccctcag gcgatgcggg ccgctacctc
     2401 atcaaggccc tttttagccg ccgggtaacg ctgcataacc agaggatcat ggcccggcac
     2461 tacatgggcg ggtgaatccg ccagggaacg cacgacgtcg aaggcaccgt acatctgcgc
     2521 gagttcgctg acgatgctgt agggacgatc ttcttcgatg ttcgcgtaga agtgcgtggc
     2581 gtccgatgcc aaaactacga agccccgagc ggtcttgacc cgcaccactt gtagccccga
     2641 cgagtgtcca cccgcccagt gaacctcgat gccaggcacg acctcctcgc ttcccgctac
     2701 gaagcgcaaa cgaccctcaa agttctccct tacgaggtac accacatcgt ctgtctccac
     2761 aatattgcgg aagtgttcct tgcccgcgta gcgaccggtc cagaaggcca tctccgcctc
     2821 ctgcactacg aaggtggcct ctgggaactt gtcgaggttc ccaacgtggt cgtagtggag
     2881 gtgggtgaga atcacatagg gaacccgcgc gcagtcgatg ccaagagcct ccagtccctc
     2941 ggtggggcag cgcaggtgct tccgtccgcg gcgcgccgct acctcggcgg tgaagcccgc
     3001 gtccacgacg atcgtgcgct cgggcgaaac caccgcccat acgtagtagt ccatcggcat
     3061 gggagcgtcg tgcggatcgc ccccgtagaa gtgctccccg ctgcgtgcct cgcgcgtcgc
     3121 gtacttgatg gcataaacct ggaatggcga tccgccactc atgtcataac gctccgtagc
     3181 cgctgctcaa acggatacct tcttgagggg aaatctcctt cgcctctggc gagcctcgaa
     3241 gaagcctagc atacgagcgg cgttcgcaac aggcgactcc gcaaggagcg ttaacgtaag
     3301 ggcgtggcaa cagaccatcg ctaggcgatg cgggttcggt gaggaggatg aaggaccgca
     3361 agcatatctt cggagaacgc ctccgcctct tccggttcta gggtggccgt ggtgacgcgc
     3421 acggcgggtg gcgcggtcaa tctatgcgct tcaccagcgc ggaccgccca ccccctttcg
     3481 agaagcccgg cgacgacaga gctctcctgg gtgaccggta tccagacatt caacccggag
     3541 gcaccgaagg ccgtgatgtt tcgcagtcgc agcgccgcga tcaacgcctg ccgccgagcc
     3601 gagtaggtac gcgcagcttg cgtcaccaga gcgtcgacgc ccggatcgtt ccatagcgcc
     3661 gccacgagcc gttgcagcac accgctgatc caaccggcgc cgagggacat ccggccttcg
     3721 acacggctga cggtgaccgg gtccccggtg agcaaagcca cccgcaagtc gggcccgagg
     3781 gtcttcgaca ccgagcgtac gaaagcccag cgagaccgcc gatcgacgag cgtgccgtac
     3841 ggagcgtcag cgctcagacc caggtggtca tcctcgacca cggtcacttc cggggccgaa
     3901 tccagcacgg cgcgcaggtc cgccgccctg gccggcgtga tgaaagcgcc cgtggggttt
     3961 tgcgcgtgcg gcgtcagcac caccgccgag accccaaagc gcagagcctc cgcaagcgtg
     4021 tcgggtatca tgccctcgtt gtccacgcgg accgggtgcc gttcgaaccc catcgccgcg
     4081 agcaggtcca gtacgccaga gtagccgggg tcctcgacgg cgacccggtc tcccggtcgc
     4141 aggtgcgcct gcagaacccg ctcgataccg tccatcgcac cccccaccac ggtgaactca
     4201 ctgccgtcca ggcctatctc ctccagccgg acatgggccg cagccgcgaa ctccgctacg
     4261 accagcggtt cccggtacag gcgcgtccgc atagccacca accggagcgc ctcttcgaga
     4321 tcgggcaaca gccggggatc ggggttgccg gtggcaaggt cgcgcgtgcc caccggaacc
     4381 ggggcctcgg accggaccct cagcggcgga cggaggctca cggtggtccc gccgcgcgag
     4441 cgccccgtca ctatgccccg agaccgtagg tctcgatagg ctgaggctac cgtagccgga
     4501 ctgacttcga gctccaccgc cagccgccga acagagggca atctctctcc cggctcaata
     4561 tcaccagtcc ttacggcagc ctcgatgccg gacgctatct ctcttgcgga ccttccagaa
     4621 aagttaaagt gtgctaatat agaattcaca tctgtactat aacagatatc gagagacgaa
     4681 accttgtggg gaggtcgcga gaaccatgtc gttacggtac agcccatcgg tggcgcgcct
     4741 cactccctac gctcctggtc tgagtgctca ggacgtggcg tccgagtttg gtatctcgcc
     4801 ttcggaagtc gtcaaactgg gctcggggga gaatcccttc gggccttctc ctgcggctgc
     4861 acgggcgatc tccgaggatc tcggcttgct caacaggtac ccggagtgga cctccgccga
     4921 gctcggccgg tcgaaaggca tgcaggatct aatagctttc gacatgggcg ggacgaccgc
     4981 gaaggtcggg gtgatccagg acggacgacc acgcttggcg cgggagttct atgccgaccg
     5041 ctacgtcggc ggggatgatt tgggcgggta tgccatcaga agccctgtca tagacctcgc
     5101 ggagatcggg gctggcggag ggagcatcgc gtggctcgac cccgccggcg tgctgaaggt
     5161 cggtccccag agcgccggtt cccagccagg accagcttgc tacggacggg gcggcgggtc
     5221 cccgactgtc acagacgccc actgcgcgtt gggcacgttg ccctccgact cctcggaggg
     5281 agagactccg agcctgcggc ccgacctcgc acgcgctgcc atcgaagcgc agatcgcccg
     5341 cccgcttggc tggaccgtag aacgatccgc gcacgcgatc ctggagattg ccacggccaa
     5401 catggcggag atggtcaggc tcgtcacggt gcggcggggg ttagatcccc gcgacttcgc
     5461 gctcttggct tacggtggcg ccggccctct tcacgcgggc gacatcgcca gggaggtggg
     5521 cgtggagaaa gtggtcattc cgccctatcc gggcatgttc agcgccatcg gaaccgtcat
     5581 gtgcgaggtt aaatacgatc tcgtccagag cctgctccgg aaggtcgatg ttctcgaacc
     5641 agagggcgtt cgagaagcct ttcgtaggct ggagcaccgg gcccagggtc tcctggccga
     5701 cgaagcagtc ccggtcgacc cggaatcggt gcgttactct cgccatcttg accttcgtca
     5761 tgagggacag ctgcacgaac tgggcatagc tttgcggcgg gacgggattc cttcgcacgc
     5821 agagattgaa aaggacttca gaaacgctta cgaggacagc tacgggtacc ggctaccggg
     5881 aagcgccgtc gagctcgtaa acgtacgcct ggaagtcgtt ttcaggctgt ggaagcccgg
     5941 gttctttccg aaggatgccc agagaataag gaccctgacc gaacgcgagc gtgcgatagc
     6001 cgaggcggac ggatcagagg tgcagtggcg tgtcctggca cgccacgagc tggttccggg
     6061 agaggaggtc tacggaccta cattgatcga agacgaagga tccgtggtcc gggttcttgc
     6121 gggccaaagc gcccggcgcg gcgatgaggg ggtgctcatc gttgaggaga ggcggtagcg
     6181 cgaagggatc catcctcgct tctgaggtcg gcttgacgac cctcgaggtc gttcgcggag
     6241 cactgggcgc tacgacggag gagatgaaga gcatcgtgat gcgggcctca ttctcgccac
     6301 ttttaacgtt atccggcgat ctatcctgcg cggtcctgga cggtaagggg caggttgtca
     6361 cgcaagggaa cgacatccct gtgcacctgg gcgccatgcc gttcaccgcc cagggtattc
     6421 tgtccaagat tccagcggag gacatagctc cgggcgacgt gctgattacc aacgacccat
     6481 acgtgggcgg aacccttctc tcggacgtca ccgtcatgtc tcccgtcttc gatgaagaag
     6541 ggttagtcgg cttcgtggcg agccgagttc actggcccga cgtagggggc gcatcggccg
     6601 gcagctcgaa ggcgacggat ggggcgatca aggaaggcct gcggatcccc ccggccaaac
     6661 tcgtagagga aaacgagctc gacgataagt tcgtcgaact attgctcgcg aacatgcggg
     6721 tgccttccga acggcggggc gatctcgaag cccagctggc gggcaaccgt cgcggggtct
     6781 ggcgtataga ggaactggta agtaggtacg gtggcggaac agttcgtgaa gtattcgagg
     6841 agtctcagcg atactcgcag atcctcgtcg aacgagcgct tcgggagatc cccgacggcg
     6901 agtaccagtt cgaggaacgt ctcgacggcg atggcttcga agaggaagat gcaccgcccg
     6961 aattgaccat ccgggtcacc gtcgcaaaaa cagggggcga aatccacttc gacttcgacg
     7021 ggtcctcgcc gtgcagccgc ggaccagtga acgcaccctt cgccgtcacc gcatccagcg
     7081 tgtactacgt cgtgctggcg gtgctcggag gccatatccc cccgaattcc ggcgcctacc
     7141 gacccgtgcg gatcatggct ccggagggga cgttggttaa cgctgcttat cctgcgccgg
     7201 tcgtggccgg caacacggag acgtctaacc ggatcatcga cgtgctcctc ggggcgcttg
     7261 ctggggccgc acccgagagg gtaacggccg gcagctacgg atccgcaggc gtctacacgc
     7321 taggcggctg ggaacctgca cgacgacgtt ccttcgtcca ctacgagaca gtcggcgggg
     7381 gtatgggagc gtttcgcggg ggagaaggcc tgagcggtat gcgggtacat atgggcaaca
     7441 ccatgaattt gccgatcgaa acggtggaat ccaggttgcc cgtgcggata cgacgatacg
     7501 aactcatctc cgattccggc ggccatggac ggtggcgcgg cgggcgcggg gttcggaaag
     7561 tggttcaggc gctctgcgac gggatcgact tctcggtctt gggagagcgc gcccgaacgc
     7621 ccgctcccgg aatctgcgga ggcgcgaccg ggaaaccggc gaacttcttc atccgaacag
     7681 gcgaaggtaa agaaattgcc ctgcggtcga aggccagctc gccgaggctt atgcaagggt
     7741 gggagctctg gctggagact gcgggaggcg gaggatacgg cgacgacgct ggtgaagctc
     7801 agggggttaa ggcacggtcc ctcccgggtg tggaaaagaa gaagatgtag gagagcgagg
     7861 aggcgtaacg atgagtgctg aaatgtcgag gctcgattac cggttgcgga tcggggtcct
     7921 acctaatggc ggagatcaac gtgtgtgctc cttcataact gaacgggagg tgatgtgagg
     7981 ggcaacgacg tcgttattgt gggagccggc aacgcggctt tctgtgcggc gcatgccgcc
     8041 cgcgagcggg ttgaacgggt ggtggtcctc gagaaggctt cgagagagtg ggctggcggt
     8101 aacacctact ttacggccgg cgcgttccgt acgacctttt ggaacctaga agagttgcgt
     8161 cctctcctcg atgacgtaag cgaagagctg ttggccaaga ctgaagtacc gccttacacc
     8221 gaggatgact tcctcgcgga tatgagacgg gtgacgcagg gacggtgcga cgaggagcta
     8281 acgggcatcc tcgttgctga ggcggccgag gccgtgcgtt ggctccacga gaagggtata
     8341 cgctggcgtt tgatgcatga gcgccaatcg tacaaggtag acggcaagag gcggttttgg
     8401 ggaggactcg tattgggaac ggtgggcggt ggcaagggcc tcgtcgagca gcacaccacc
     8461 gccgcaagca ggactggcat cgagatccgt tacgggtgtg aggtgaccgg ccttctgcgg
     8521 gacgattcga gggctgtggt cggggtggcg tgcgaaggcc ccgaagggcg agaggagatc
     8581 gaagccgggg cggtggtact cgcgggtggt ggtttcgagt ctgacccgca ggcgcgcgcg
     8641 cagtaccttg gacccaactg ggacatagcg aaggtgcgcg gcacgccaca caacacggga
     8701 gaggtattgc gcatggctct ggaggccggc gcccaaccct acggtcattg gagcggctgc
     8761 cacgcgatag cctgggacgc tgccgcgccc ccggtcggcg atcgcgagct aacgaacctc
     8821 ttctccaagc agtcgtaccc gctcggaatc ctcgtcaacg cggagggaca gcggttcgtg
     8881 gacgagggtg cggacttccg caactacaca tacgccaagt acggcgccga gatcttgagt
     8941 cagccagggg tgatcgccta ccagctcttt gattcgaaga cagaatcgct gctgcgtcag
     9001 gatgaataca ccgccccggg cgtgtctagg ttcgaggccg acacgatgcg tgcgcttgcc
     9061 gaagagatag gggtggctcc ggaggccttg gaaaagacaa tcgaagagtt caacgccgcc
     9121 gtccaacccg gcgaattcga tccgactatc agagacggca agtgtacgga aggcgtcgag
     9181 ccgcccaagt ccaactgggc ccagcctctc gactctccac ccttctacgg gttcgcggtt
     9241 acgtgcggca tcaccttcac cttcggtgga gtgcgcatag acgaggccgc ccgggtgctg
     9301 gacagagcgg gccggccgat aaagggtttg cacgccgccg gagagctcgt cggcggcctt
     9361 ttctaccaca actaccctgg aggaagcggg ttaacctccg gcgcggtgtt cgggcggcgc
     9421 gcgggggcta cggccgccac gcatgccggt gctcggacgt ccggcttccg taaccttgcg
     9481 caggagaatt cgaggtgagc gtaagggatc gtaaggtcgg tttcgtgggt ctgggtaaca
     9541 tgggcggcgc catggtggag gctatcttgg cggacggcta ccgggtctgg gtgtacgaca
     9601 tcgatgcggc ggcggcttct gcattggaac ctctaggggc tcaagaggcc cctggtcccg
     9661 gagccctagc ggcccaggca gacgtcattc tcaccagctt gcccgatgcc cgagctgtac
     9721 gggctgtcta tctcggggaa gaagggctcg tctcgggggc gcgtcccggc accgtgctcg
     9781 tggagttgag caccatcgat cccgccacga tcgaggagct cgaacgggtg gtctcggagc
     9841 ggggagcggt gctcctcgat gtccccgtga gcggtagccc ggacgaagct cgccgggggg
     9901 agctggtgct taccgctggg ggagagcggg aggcgctcgc ggccgtcgag cctatactga
     9961 attgctttgg gagaacgatc cagtacgtcg gacctgcggg cgcggccaag atcgtcaaga
    10021 tagtcaacaa tatgatgacc atgggtaatg tgctggtcgc ggccgaagcc ttctgcgtgg
    10081 gggtgaaggc gggaatcgct ccggacctcc tattcgaggt tctaagcggg agcggtggac
    10141 gctctcacca cttcacgaaa cgtttcccca acgctctggc cggcgatttc aaaccgggct
    10201 tctcggtcgc tttgggagag aaggatctag gactcgcgct agagctctct cggagcctgg
    10261 acatgccttc gcccacgacc ggcttggtgc gccaggtgta ccggatcgcc atgtcggagg
    10321 gactctcctc ggaggacatc gtggccgtta taaagctctt cgagaaatgg agtggggtcc
    10381 aagctcgagg cgcggctcgt cgggaggcca gcggtggcga ccacgaggtc gaggcggttg
    10441 gcgaggggcc aaatttgacg acggagctgt ttcgtcggat agaggccaag ctgaacgaag
    10501 ac
//
LOCUS       DATMJA010000299         3501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_513639, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000299 DATMJA010000000
VERSION     DATMJA010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513639"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VNT12_07165"
     CDS             complement(<1..337)
                     /locus_tag="VNT12_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWI45936.1"
                     /translation="MAVVKRRPLSRQRILEAAVRFVDREGLEALSMRKLGSELGVEAM
                     SLYNHVPNKGVLLEGMVEILLGKLQIPPESEGWERRVKDAYRAFRRLAHEHPNVFPLL
                     LVRPPDTMDG"
     gene            complement(633..914)
                     /locus_tag="VNT12_07170"
     CDS             complement(633..914)
                     /locus_tag="VNT12_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45937.1"
                     /translation="MERPVTQATGHNNTIVSVYEDRVELRTGWQSQNVESVGLRDVAV
                     IGLKGLVNVTLTIETNTGRVYQLNRMALPDARRIKNTFEQQKRRAGLYE"
     gene            complement(1040..2455)
                     /locus_tag="VNT12_07175"
     CDS             complement(1040..2455)
                     /locus_tag="VNT12_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWI45938.1"
                     /translation="MRRLALLSTTVLFLVLALPGLAFAQKGPSTADLSLAMDTMWVIV
                     AACLVLFMQAGFAMLEVGFSRMKNVGSVVAKILVNLAIAALLFWAFGFALAFGSGNAL
                     LGTEGWFLAVPSGQVNDVYAGLSWTQVPLSAKFLFQVAFCAVSLAIVWGTMLERTKFA
                     VYVVFAIVFAGLIYPMVGHWIWGGGWLTELGMQDFAGSTVVHLSGAMAALAGTLLLGP
                     RLGKYDDDGRPVNIPGHNMPLAVLGVLILWIGWWGFNPGSTMAAVPDIADIALTTNLA
                     AAAGVLGAMGMSYLYRRNVDVGMGGNGAIAALVAITAPCAFVAPWASIVIGCVAGIIM
                     YTTLVYVDRIGVDDPLGAIAAHGMGGIWGTLSCGLFTTPALAAVGEPGLFYGGGLYQL
                     GIQALGIVAAGGFVFLASLAVFATLKATIGIRVKPEQELDGLDIHEHGVFGYPDLVAT
                     DYQNGNGKISDPMEGVPEGTA"
     gene            complement(2610..>3501)
                     /locus_tag="VNT12_07180"
     CDS             complement(2610..>3501)
                     /locus_tag="VNT12_07180"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI45939.1"
                     /translation="ERIRLETELGARGDFVDDLVSGHYGSVELLLQRARYLGADIAGG
                     ALVMVVDIDDFARYLARRKPKEPAIQELKRRLADAVRLQTRQLFSNFLLGPRSDNVIL
                     FLGRAEGEGPEELPEKAQLLAKGIQRYVKGLLPDLTVSVGIGGFKQDPSQLPEAYSEA
                     GVALEIGHRIHGPSSVSTFGKTGTYKLLFRVLQENPEELEAFYGETLEPVVHYDSRYG
                     TELVQTLITYLGNDASTVRTAGDLFAHRHTIRYRLDRVSELTGLDVDKSEDRERLTLG
                     IKAMQLLGRVPEGPSSFADR"
BASE COUNT          678 a         1059 c         1116 g          648 t
ORIGIN      
        1 ccccgtccat ggtgtccggc ggacggacga ggagcagcgg gaagacgttc gggtgctcgt
       61 gggcgagccg ccgaaacgcc cggtaagcgt ctttgacccg tctctcccaa ccttcgcttt
      121 cgggcggtat ctgaagtttg ccgagcaaga tctcgaccat cccttccagc aagacaccct
      181 tgttcggcac gtggttgtag agcgacatcg cctcgacccc aagctcactc ccgagcttcc
      241 gcatcgagag agcctccaac ccctcccggt ccacaaaccg taccgcagcc tcgagtatcc
      301 gctgcctgct caacggccgc cgcttgacaa ccgccatcac aaacctccct ctgctagaac
      361 gcttgatagc ttacagcatg agcgccgtgt ttacgacgta agcatgccga ggcggaggat
      421 agagaggggt aaggcctggg gagtggaaag gcaccccgaa tggttacggt tgtgtcactc
      481 tggggtgact ggctgtagtg tactagagcg attctcgcag tggtatcgac ttaccgtgaa
      541 tcgctcattc gagtaggagc catcggtgaa aacgaaaagc tgaaatgctg acgagcggag
      601 gcatcagccg gagcgtgctg aagtgcttgt ctctactcgt aaaggccggc cctacgtttc
      661 tgctgttcga aggtgttctt gattctacgc gcatccggga gggccatgcg attcagttgg
      721 tagacgcgac ccgtattagt ctctatcgtc agggtgacgt tgacgaggcc cttgaggccg
      781 atcacggcga cgtcgcgcag gccgacgctc tctacgttct gggattgcca gccggtcctc
      841 agctcgaccc ggtcctcgta gacgctgacg atggtgttgt tgtggccagt ggcttgcgtt
      901 acaggtcttt ccaagctgtc ctccgtgccg cctgcggacc gcctctaaca tcaggataca
      961 agacggcgtt accgatttgg ggtggacgtg aaaaaacacc ggccggtgga gatccggccg
     1021 gtgccaaagt gtttctgccc taggctgtac cttctggcac gccttccata ggatcggaga
     1081 tctttccgtt cccgttctgg taatcggttg cgacgagatc ggggtagccg aagactccgt
     1141 gctcgtggat gtcgagcccg tcgagctcct gctcgggctt gaccctgatg ccgatggtgg
     1201 ccttgagcgt cgcgaagacg gcgagcgagg ctaggaagac gaagccccct gccgcgacga
     1261 taccgagagc ctgaataccg agctggtaga ggccgccgcc gtagaacagg ccgggctcgc
     1321 cgacagcggc gagggccggg gtggtgaaca ggccgcagga gagcgtgccc cagataccgc
     1381 ccataccgtg cgccgctatg gcgccgagcg gatcgtcgac cccgatccta tccacgtaga
     1441 ccagtgtggt gtacattatt atgccggcga cgcagcctat cacgatcgag gcccaaggcg
     1501 cgacgaaagc gcagggcgcg gtgatggcga ctagggccgc aatggcgccg ttgccgccca
     1561 tcccgacgtc tacgttgcgc ctgtagaggt agctcatgcc catcgccccg aggaccccgg
     1621 cggccgcggc gaggttcgtc gtcagggcga tgtcggcgat gtcgggaacg gcggccatcg
     1681 tcgatccagg gttgaagccc caccagccga tccagagtat gaggacgccg aggacggcga
     1741 gcggcatgtt gtggcccggg atgttcaccg ggcggccgtc gtcgtcgtac ttgccgaggc
     1801 gcggaccgag gagcagagta ccggcgagcg ccgccatcgc gcccgagagg tggacgaccg
     1861 tcgagcctgc gaagtcctgc atgccgagct cggtcagcca gcctccgccc cagatccagt
     1921 gtcccaccat cgggtagatc aagcccgcaa agacgatggc aaagaccacg tagaccgcga
     1981 acttggtcct ctccagcatc gtgccccaca cgatggccag tgagactgcg cagaacgcaa
     2041 cctggaagag gaacttggcc gagagcggga cctgtgtcca ggaaagtccg gcgtagacat
     2101 cgttgacctg cccggacgga accgcgagga accagccctc ggtgcctaag agcgcgttac
     2161 ccgagccgaa ggccagagcg aagccgaagg cccagaacag cagggccgcg atcgccaggt
     2221 tgacgagtat cttggcgacc acactcccga cgttcttcat tcgggagaaa ccgacctcca
     2281 acatagcgaa tcctgcctgc atgaagagga ccagacacgc cgctacgatc acccacatcg
     2341 tgtccatcgc cagcgagaga tccgccgtcg aaggaccctt ctgcgcgaac gccagcccgg
     2401 gtagcgcgag caccaaaaac aagaccgtgg tcgataataa cgcgagcctc cgcaaaagct
     2461 tcctccatat cttcagggta tattctcttg aaacatagtc taggcaccgg ccacagaagg
     2521 gtctttagcc gaatgtacaa agatgggtgc cccgctcgaa gctcccgttc tagcggatgt
     2581 acaaggaggc gggggagcat gctgacatgc tatctatccg cgaagctcga tggtccttcg
     2641 gggacgcggc ccaggagttg catcgccttt attccgaggg tgaggcgttc gcggtcttcg
     2701 ctcttgtcga cgtcgaggcc tgtcagctcg ctgacgcggt cgaggcggta gcggatcgtg
     2761 tggcgatggg caaagagatc gcccgctgtc ctgacggtcg aggcgtcgtt gccgaggtag
     2821 gtaatcaggg tctgaaccaa ctcggtcccg tagcgggagt cgtagtgtac gacgggctcc
     2881 agtgtctcgc cgtagaaggc ctcgagttcc tcggggttct cctggagcac cctgaagagg
     2941 agtttgtatg tccctgtctt cccgaaggtc gagacgctgg atggcccgtg gatgcggtgt
     3001 ccgatctcga gcgctacccc ggcctcggag taggcctcgg ggagttggga ggggtcctgc
     3061 ttgaagcctc cgatcccgac ggagaccgta aggtcgggta ggaggccttt gacgtagcgc
     3121 tgtatccctt tggccagcag ttgcgccttc tccggcaact cttcgggacc ttcgccttcc
     3181 gcacgaccca gaaaaaggat aacgttatcc gagcgtgggc cgaggaggaa attcgagaag
     3241 agctggcggg tctgcaagcg taccgcatct gccaggcgtc gcttgagctc ctgaatggcg
     3301 ggctccttcg gtttgcggcg tgcgaggtag cgggcgaagt cgtctatgtc gacgaccatt
     3361 accagggccc cgccggcgat gtcggcgccg aggtatcttg cgcgctgcag gaggagctcg
     3421 actgagccgt aatggccgct cacgaggtcg tccacgaagt ctcccctggc acccagttcc
     3481 gtctccaggc gaatacgctc c
//
LOCUS       DATMJA010000300         3692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_572888, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000300 DATMJA010000000
VERSION     DATMJA010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572888"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VNT12_07185"
     CDS             <1..452
                     /locus_tag="VNT12_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI45940.1"
                     /translation="ATTEDAGGIHGLARELAETVGDEPPTEEAIRARLEELLDEPRAR
                     VLVAENEAGIVGGASFWIKPDLAHGDTVIEVPMLVVAEDHRRAGVGKLLMEEVRNVAS
                     DNGASQIELVATRANVTAREFYRSLGFVEADVVSLEFVGSQEDPPEE"
     gene            complement(465..1004)
                     /locus_tag="VNT12_07190"
     CDS             complement(465..1004)
                     /locus_tag="VNT12_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45941.1"
                     /translation="MAKRGYTRDTIRGGASEWDVSRPLSSYARTLAMLLVHPVRFFEL
                     LPRIPDMRAPALFLIFSGLPAAILWLIFWGPYPALVAVGLPLPLSFLLAGLYHLGALG
                     GRHGYVVTWRVLAYPLGFYLPFAAVPLLGQLGAAYSGLVLMGLGLAEVQEIARLWTGL
                     FCTVVVAVFGAAYYFLSVA"
     gene            1068..2684
                     /locus_tag="VNT12_07195"
     CDS             1068..2684
                     /locus_tag="VNT12_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HWI45942.1"
                     /translation="MNPSSGDRIKPLRRRSAALLGGAVAIFVGILYIGTLAPTVLPYG
                     TPDTLDSPMLQTEVTVLGVGHPTGYPTYMMLTHLFTYLPIGDPAYRVNLASAVYGVAA
                     VFAVYLAGLRLGGRAVAAAAGALAFGLSGTFWSQAVIAEVYTLEALLVALVILFLLAW
                     RDRGDGRYLLLCAFVIGLSLTHHLTSVLLVPAALVFVFLTDRRVFSRTGLMLKSLGFF
                     LLGLIPLLYLPIRALMHAPLNEADPSTLWRFLLLVTGGSFLAESSEKERQCSPSSLAL
                     ADPAIKLQLLGDHLLGQFPLFLIAVGALAAVYLLFTDRATAVLLGTLFLGCLVQAAVY
                     LQLGIEDFYVFLIPALLAFGLLISAGLGTLLRLAESLVVDSAARKGLLVVLSGLMLIV
                     PLPGVRDSYAAHDRSGDYGGRRVMEAVAGNVEKGATILHHRSPLWYMVLVERRRRDLT
                     LMDPFCTSWDRHTDVVWPDQISASEAADHYGTDDTTGVEAARRAAGTGPVYLLDNGRT
                     RLNSFREAGFDVVPVEKDGFLYELVPRSRG"
     gene            2780..>3692
                     /locus_tag="VNT12_07200"
     CDS             2780..>3692
                     /locus_tag="VNT12_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWI45943.1"
                     /translation="MTNDPFSARTTLKTGSGDVELYRLKTLDESVDGDVFSLPFSIRV
                     MLESLLRNAGGKFVSKEDVEALASWPESVGSELAYLPARVVMQDFTGVPAVVDLAAMR
                     SAMESVGGDPGKINPLVPTDLVIDHSVQVDAFGTPAALQFNAEREFERNRERYEFLKW
                     GQQAFDNFSVVPPATGIIHQVNLEYLAKGVIVKDGVAMPDTLVGTDSHTTMINGLGVL
                     GWGVGGIEAEAVTLGQPYYMLVPEVIGFKLTGALQEGVTATDLVLTVTQMLRSHGVVG
                     KFVEFYGDGLSKLSLPDRATIANMSPEF"
BASE COUNT          673 a         1164 c         1158 g          697 t
ORIGIN      
        1 acgcgaccac cgaggacgcc ggtgggattc acggtctcgc ccgcgagctg gccgagacgg
       61 taggcgatga gccgcccacg gaagaggcca tcagggcgcg gctcgaagag ctcctggacg
      121 aacccagggc gcgggtcctc gtcgccgaga acgaggccgg gatcgtggga ggggcgagct
      181 tctggatcaa acccgacctc gcccacggtg acaccgtcat cgaggtgccg atgctcgtcg
      241 tcgcagagga ccacaggcgg gccggggtgg gcaagctcct tatggaggag gtccgcaacg
      301 tcgcttccga caacggcgca tcccagatcg aactggtcgc cacccgcgcg aacgtaacgg
      361 cccgtgagtt ctaccgctcc ctcggcttcg tcgaggccga tgtggtctcg ctggagttcg
      421 taggatctca ggaagacccg cccgaagaat gaccttcgcc cggatcaggc aacgctcagg
      481 aagtagtaag ccgccccgaa tacggctact acaaccgtac agaagagccc ggtccagagc
      541 cttgcgatct cctgcacctc agcgagccct agccccatga gcacgagccc tgagtaagcg
      601 gctcccagtt gcccgagcaa agggactgcg gcgaagggca ggtagaaacc gagcgggtaa
      661 gcgaggaccc tccaggtcac cacgtacccg tgccggccgc cgagagcgcc tagatggtag
      721 aggccggcga ggaggaagga tagcggcaat ggcagtccga ccgctaccag cgcaggatag
      781 ggcccccaga agatcagcca caagatcgcg gcggggaggc cggagaagat gagaaagagg
      841 gcaggcgccc tcatgtccgg aatccttggc aagagctcga agaatctgac cgggtgcacg
      901 agtagcatgg ccaacgtccg cgcgtagctc gaaagaggcc gcgagacatc ccactcgctg
      961 gcgccgcctc ggatcgtgtc ccgcgtgtag ccgcgcttag ccacgggccg agtatacaga
     1021 agcccatcag gtagggcggt gcaagtacgt agtattctgc ccctgcgatg aatccttcct
     1081 cgggtgacag gatcaaacct ctcaggcggc ggagcgcggc tttgctcgga ggggcggtcg
     1141 cgatcttcgt cggtatcctc tacatcggga ccctggcgcc cacggtcctg ccttacggta
     1201 ctcctgacac cctcgactcc ccgatgctcc agaccgaggt cacggtgctc ggtgtcggcc
     1261 atccgaccgg ctacccgacc tacatgatgc tgacgcacct ctttacttac ctgccgatag
     1321 gcgaccccgc ctacagggtg aacctcgctt ccgccgtcta cggcgtcgcg gctgtgttcg
     1381 ccgtgtacct cgcgggactg aggcttggag gacgggctgt cgccgcggcg gccggggcgc
     1441 tggccttcgg gctttccggg accttctgga gccaggctgt tatcgccgag gtctacaccc
     1501 tcgaggcact cctggtcgcg ctcgtcatcc tcttcctgct cgcctggaga gaccgcggcg
     1561 acggccgata cctgctgctc tgcgcctttg tgattggcct ctcccttacc caccacctga
     1621 cgagcgtcct gctcgttcct gccgcgctgg ttttcgtgtt ccttaccgac aggcgtgtgt
     1681 tttcgaggac gggtctgatg ctgaagagtc tcggattctt cctgctaggc ctcattccgc
     1741 tcctttacct cccgatacgg gccctgatgc acgcgcctct gaacgaggct gatccctcga
     1801 ccctctggcg cttcctgcta ctcgtcacgg gcggcagctt tctggctgaa tcttcggaga
     1861 aggagaggca atgcagccct tcgagcctcg cgctcgctga cccagctatc aaactacagc
     1921 tgttggggga ccatctcctc gggcagttcc ctctcttcct gatagctgtc ggggcactgg
     1981 cggccgtcta cctgctattt acggaccgtg cgacggccgt acttcttggt acgctcttcc
     2041 tcgggtgtct cgtccaggcc gccgtctacc tccagttggg gatcgaggac ttctacgtgt
     2101 tcctcatccc cgctcttctg gctttcggac tgctaatctc ggcaggcctc ggaacattgc
     2161 tgcggttagc agagagcctc gtggtcgact cagctgcgag gaagggcctg ctcgttgtcc
     2221 tctcgggcct gatgctcatc gtaccgcttc cgggcgtgcg cgactcctat gccgcgcacg
     2281 atcgtagcgg ggattatgga ggacgcaggg tgatggaagc cgtggccggc aacgtggaga
     2341 agggcgcaac tattctgcat cacaggagcc cgctctggta catggtgctt gtcgagagga
     2401 ggaggcggga tctcaccctc atggaccctt tctgtacatc gtgggaccgc cacaccgatg
     2461 tcgtctggcc ggaccagatc agcgcgtccg aggctgctga tcattacggt accgacgaca
     2521 caacaggcgt cgaggctgca cgcagggctg ccggaaccgg gcccgtctac ctcctggata
     2581 acggccgtac ccgactgaac tcctttcggg aggccggttt cgatgtggta ccggtcgaaa
     2641 aggacggttt cctctacgag ctcgtgcccc gaagccgcgg gtagactacg ctagaggtgg
     2701 gcatgtcccg tagggcatcc cggatcatct ataatcggat aaggccacgc atacgagttt
     2761 cggagatgag gttacgaaca tgacgaacga tcccttcagt gcgcgcacga cgctgaagac
     2821 tggttccggt gacgtagaac tctatagact taagaccctg gatgagagtg tggacggaga
     2881 cgtgttctcg ctgcccttct ccatcagggt gatgctcgag tccctcctac gcaacgccgg
     2941 tgggaaattc gtctccaaag aagacgtcga ggcgctggcc agctggcccg agtcggtcgg
     3001 cagcgagctt gcgtatctgc cggccagggt cgtaatgcag gacttcaccg gggtacctgc
     3061 ggtcgtcgac ctggcggcta tgcggagcgc gatggagagc gtgggcggag atcccgggaa
     3121 gatcaaccct ctcgttccaa ccgacctggt tatagaccac tctgtccagg tcgacgcttt
     3181 cgggacgcct gcggcactcc agttcaacgc tgagcgggaa ttcgagcgca accgcgagcg
     3241 ctacgagttc ctgaagtggg gccagcaggc attcgacaac ttctcggtcg taccgccggc
     3301 cacggggatc attcaccagg tcaacctgga gtacctggcg aagggtgtga tcgtaaagga
     3361 tggcgtagcg atgccggata cgctcgtcgg cacagacagc cacaccacca tgatcaacgg
     3421 cctcggcgta ctcggttggg gcgtcggggg catcgaggcc gaggccgtca cccttggcca
     3481 gccctactac atgctcgtcc ccgaggtgat tggcttcaag cttacgggcg ctctacaaga
     3541 gggcgtcacg gccacagacc tcgttctcac agttacccag atgctgcgat cccacggcgt
     3601 agtaggcaag ttcgtggagt tctacggcga tgggctcagt aagctctcgc tacctgacag
     3661 ggccaccatc gcgaacatgt cccccgagtt cg
//
LOCUS       DATMJA010000301         9412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_98996, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000301 DATMJA010000000
VERSION     DATMJA010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98996"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            656..3142
                     /locus_tag="VNT12_07205"
     CDS             656..3142
                     /locus_tag="VNT12_07205"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWI45944.1"
                     /translation="MEKTNEKERLERIDIPVTGMTCASCVRRVERALSKQEGVTEASV
                     NFAAEKASVTYEPTTRSPDELIWAIQDVGYGADVRQMTLGVTGMTCASCVGRVEQALK
                     KVPGVLEASVNLTNEKATVEYLASEVELRDLEKAVEGAGYGVVREESSVGDTREREYE
                     KLRADFLLAAGLTTLILVGSLPMMFGFMLPVPLGWLNFGLLLLVTPVQFWAGWRFYRG
                     AWGALKHGQANMNTLVVMGTSAAYLYSAVATLAPGLFAAGRADVYFDTSALIITLILL
                     GRLLEARAKGRTNEAIKKLAGLQAKIARVLRDGEEVDVPVEDVDVGEVVVVRPGEKVP
                     VDGRVISGESAVDESMITGESIPVTKRAGDEVIGATMNTSGSFRFTATKVGEETALHQ
                     IMRMVEEAQGSKAPIQRLADRISAVFVPAVIGVAAVTFLIWFLFGPEPALTFALLNTV
                     AVLIIACPCAMGLATPTSIMVGTGKGAESGILIKGGEALEGAHKLDTVVLDKTGTLTR
                     GVPELTDLVVGDGIDEVELLRLVASAEWTSEHPLGEAIVRGAKERALSLVEAEGFEAL
                     SGSGVRARVGGREVVVGNRRFLSELGVSEDCLLPKGEELAREGKTLVFVAVDGEPAGL
                     VAVADVVREESREAIERLHSLGLEVAMLTGDNRRTAEAIARQLGVDRVVAEVRPQDKA
                     NEIRRLQAEGQKVGMVGDGINDAPALAQADVGIAIGTGTDVAMEAADLTLISGDVRGV
                     ARAIDLSKATVRNIKQNLFWAFAYNVALIPVAAGVLYHLFPDGSVPEILRPVLGEYGF
                     LNPVLAAAAMALSSVTVVSNALRLRRVKVG"
     gene            3160..3477
                     /locus_tag="VNT12_07210"
     CDS             3160..3477
                     /locus_tag="VNT12_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWI45945.1"
                     /translation="MDATQDNEQVPAHGYIKARDKEVLQNRLRRIEGQVRGVQRMVDE
                     EAYCVDVLTQIASVVAALEKVGTILLKDHVEHCVRESIEKGEDAGEKIEELTAAVERF
                     LRV"
     gene            3507..3716
                     /locus_tag="VNT12_07215"
     CDS             3507..3716
                     /locus_tag="VNT12_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HWI45946.1"
                     /translation="MTEKTLKVPDMSCAHCKAAVEGELNRLSGVQRANADVDKGTVEV
                     TYDEGKVTTEDLKGAIEEAGYGVAA"
     gene            complement(3901..4200)
                     /locus_tag="VNT12_07220"
     CDS             complement(3901..4200)
                     /locus_tag="VNT12_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45947.1"
                     /translation="MCGEANPQRPTLGEQPQKQPEKADGEHGEVLGARPMSGSMIRGV
                     LVCGTHRTGRGLVLRCVIRVTGALRRVADQVQGPEDPVAEEQRADDEHGQLPDAE"
     gene            4419..4904
                     /locus_tag="VNT12_07225"
     CDS             4419..4904
                     /locus_tag="VNT12_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45948.1"
                     /translation="MPPGAYGYKVLVTVLFSRTMPLIRYEMSDSVRLASSPLCPCGRP
                     FAFIDGIQGRAEDVLRFPATSGGQVSVQPIIFHRVMDTVPAGGWQVVQSSEGLTVLLS
                     GVREGFAEATLIDSLRHELGAQGAIVPPVKVRHVPTIPRTTVGKAPLIKARVPGPRRK
                     P"
     gene            4945..5556
                     /locus_tag="VNT12_07230"
     CDS             4945..5556
                     /locus_tag="VNT12_07230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI45949.1"
                     /translation="MSRKWWFILAAMLLTATLYRNRLRAYLEQASANVRDFDLPSAGA
                     YDALVATVLEGLYARVAGEVGAAHPGGNLLEVGSGPGRLAVRLAREAPGMTLTGVDIS
                     DVMVELAARRAARAGLSERVRFEVGDVAVLPLSDEDVDGVVSTSPCTTGRTRPAGGPR
                     STGSSSPAGRHGSTISPTGCGDLRGKGTDSPSWQPRARSARGK"
     gene            5671..8124
                     /locus_tag="VNT12_07235"
     CDS             5671..8124
                     /locus_tag="VNT12_07235"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWI45950.1"
                     /translation="MTNNDPRAVATADHQHVHVGRGTFELRGVYCLCCAGAVERALKE
                     QPHVIDVRLDWKNDVVHVGYDPARIGQEDIEDVITQTGCDCNPTGVEEEHHHEVMAPP
                     ERRMQHLGHGVDTQPISMGTKHDRMQYEMPATHADHGDHMDHDMSDPLMAATMERDMR
                     NKFFVALLLTIPTVLYSPLGMNLLGVRLPTFGLGENLIMLLLSTPVVFYSGWIFLSGS
                     YHSLRRRTLNMSVLIATGVLAAYLFSVLITFLGGESFYEAAAMLVTFVLFGHWMEMRS
                     RRGTNDALRALFDLVPPQATVIRDGEEMTIPSSEVQLGDTVVLRPGDKVPVDGEVIEG
                     ETSIDEALVTGESVPVTKRPGDAVIAGSINRSSSVRFEATAVGAHTTLAQIVDLVQKA
                     QNSKAPGQRLADRAAEYLVILAVGSGIITFFAWYFLGGAAAITAMTFAISAVVIACPD
                     ALGLATPTAVAVGTGIGARHNILIKDAATLENVSRIDAIVLDKTGTLTEGEPSLTDVY
                     TLDGRSEVDLLRLVAPAEKNSEHPLAEAIVAGARDRGIEPKDASRFEAVAGHGIRAEV
                     DSHVVLVGNRKLMDESEVDVFALEERAVTLAEAGKTPMFVAVDGQPSGLVAVADTIKP
                     SAPETLRRLKQMGIEAVMITGDNRRTAEAVARELGIERVFAEVLPADKARYVEELQEE
                     GKHVAMVGDGVNDAPALAQADIGIAIGAGTDVAIETAKVVLMKSDPLDVIRAVVLSKG
                     TVRKMKQNLVWASVYNVLAIPIAAGVLFPAYGIMLRPEWSALLMSVSSIIVAVNAVLL
                     KGVESELEEPKSVDGIVPE"
     gene            complement(8425..8661)
                     /locus_tag="VNT12_07240"
     CDS             complement(8425..8661)
                     /locus_tag="VNT12_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45951.1"
                     /translation="MSDGLKVLLGVLGGAVLVLLLISVLGGGGMMGSMGSMMSGGMMG
                     GGLFGMLFVLLFWVLVIALIVALVVRIVTQTQRR"
     gene            complement(8796..>9412)
                     /locus_tag="VNT12_07245"
     CDS             complement(8796..>9412)
                     /locus_tag="VNT12_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWI45952.1"
                     /translation="EHMNHMEMGHHHHMDHQMGPARLPDGSEFHLVRLVVAEKTDYSR
                     SVPETLSEIPQADLDRADTRPITISMTTDDQGMRWLINGNTHRMDEFPIVVQRGAREI
                     WEIHNDEKSMPHPAHLHGFQFRVMERAGTPEQVAHLAVDEKGRMVTDVGSKDTVLLWP
                     GETVKCAMDFSHSFEGEQLYMFHCHILEHETGMMLNLKIVDPPG"
BASE COUNT         1802 a         2863 c         3054 g         1693 t
ORIGIN      
        1 ggtatttcca gttccacgag ggcggtaagc gtaagaagct ctacctcggc aagactagcg
       61 acccggagag tactttggct gccaagcgag catagctctc aggcaaatag attgctaccc
      121 caactcttca cgtagcccag ctttcagcgt tgtgcacctt cagcatgccc acctgcgcca
      181 agcggagtat ctcgtcatgc tcgcaggcaa tccgaagggt cgatttggag gacgagagca
      241 gatctttgcg gtaggctaga ggctggacga tccagcgggg tgcagtacgc ttggcgcaac
      301 ttcggcgagg acgagcaacc gtgtctcgca ggcgatctaa gaaaccgtct ctgcaaccgg
      361 gctaatacgc gctggcacgc aacgcacacc agagcggttc tagtacgccg aaatccgcgt
      421 aaataagccg attcaccgga cgcaccagga ctggctggaa cgtaccgaag gcaagttcgt
      481 aaagaggtta ggtcagcact tcgagtccgc tcgtcggctc tccgtattcg ggatagataa
      541 gagtgtgtcg cgctccctcc gcgacacgga gatgagaagc acctggcttg acctataggg
      601 tacaggggta tagcataaag ccaactcgaa cgagcgtgaa ggcgcggagc actacgtgga
      661 gaagaccaac gagaaggagc ggcttgagcg aatcgacatc cctgttacgg ggatgacttg
      721 cgcctcctgc gtgcggcggg tcgaacgagc actttccaag caggaggggg tcaccgaggc
      781 gagcgtgaac ttcgcagccg agaaggcgag cgtgacctac gaacccacca ccaggagccc
      841 ggacgagttg atctgggcca tacaggacgt cggctacggc gctgacgtgc gccagatgac
      901 cctcggcgtg acaggcatga cgtgtgcgag ctgcgtgggg cgggtcgaac aagccctcaa
      961 gaaggtgccg ggcgttctgg aggcgagtgt gaacctcacc aatgagaagg ctacggtcga
     1021 gtaccttgcg agcgaggtgg agctgcgaga cctggagaag gctgtcgagg gcgcaggcta
     1081 cggcgtggtg cgggaggagt cctccgtcgg ggatacccgc gagcgcgagt acgagaaact
     1141 cagggcagat ttcctcctcg cggctgggct cactacgctc atcctcgttg gcagcctccc
     1201 gatgatgttc gggtttatgc ttccggttcc gttggggtgg ctcaactttg ggttgctcct
     1261 ccttgtcacg ccggtgcagt tctgggctgg gtggcggttc taccgggggg cgtggggcgc
     1321 gctcaagcat ggccaggcga acatgaacac gttggtggtc atgggcacca gcgccgccta
     1381 cctctacagt gctgtggcga cgcttgcgcc tgggctcttc gcggccggca gggccgacgt
     1441 gtacttcgac acctcggcgc tcatcatcac cctgatcctt ctggggcgcc tgctcgaggc
     1501 gcgggcgaag ggtcgcacca acgaggccat aaagaagctt gccggtctcc aggcgaagat
     1561 cgcccgcgtt ctgcgggatg gggaagaggt agacgtaccc gttgaggatg tggatgtcgg
     1621 ggaggttgtg gtcgtcaggc ctggggagaa ggtgccggtt gacggtcgcg tcatctcggg
     1681 cgaatcagcg gtggatgagt ctatgatcac cggcgagtcc atccccgtca ccaagagggc
     1741 gggcgacgag gtcattgggg cgacgatgaa cacgagcggg tcgttccgct ttaccgcgac
     1801 aaaggtcggt gaggagaccg ctcttcacca gatcatgagg atggtcgagg aggcgcaggg
     1861 ctcaaaggcc ccaatccagc gcctcgccga caggatcagc gcggtgttcg tgcccgcggt
     1921 gatcggcgtg gccgccgtta cgttcttgat ctggttcctg ttcgggcccg agccggcact
     1981 cacgtttgcg cttctgaaca cggtggcggt cctgatcatc gcctgcccgt gcgctatggg
     2041 gcttgcgacg ccgacctcca taatggtcgg caccggcaag ggcgccgagt ccggcatcct
     2101 catcaagggc ggcgaggccc tcgagggcgc ccacaagctc gacacggtcg tactggacaa
     2161 gacgggcact ctcaccaggg gcgtgcccga gctgaccgac ctggtcgtcg gggacgggat
     2221 cgacgaggta gagctcttga ggctcgttgc ctccgcagag tggacgagcg agcaccccct
     2281 cggcgaggcc atcgttagag gtgcgaagga gcgggctctc tccctcgtcg aagccgaggg
     2341 gttcgaggcc ctctcgggta gcggcgtccg cgctcgcgtc gggggtcgcg aggtcgtggt
     2401 cggtaaccgt cggttcctct cggagttggg tgtctcggag gactgcctcc tcccgaaggg
     2461 tgaggagctg gcgcgggagg gcaagaccct cgtgttcgtc gccgtagacg gcgagcccgc
     2521 cggcctcgtg gcggtcgcgg acgtagtgcg cgaggagtcc cgggaggcca tcgagcggct
     2581 gcactcacta gggctcgaag tcgccatgct caccggcgat aaccgccgca ccgccgaggc
     2641 gatcgcgcgt cagctcggcg ttgaccgcgt cgtggcagag gtcaggcccc aggacaaggc
     2701 gaacgagatc aggaggttgc aggcggaagg ccagaaggtg ggcatggtcg gagacggcat
     2761 caacgacgct ccggcactcg cccaggccga cgtcggcatc gccatcggca ctggtaccga
     2821 cgtcgccatg gaggcggccg acctgacgct catctccggc gacgtgcgcg gggtggcgag
     2881 ggcgatagac ctttccaagg ctacggtgag gaacatcaag cagaacctgt tctgggcctt
     2941 cgcctacaac gtcgccctca tccccgtagc ggccggggtc ctctaccacc tcttccccga
     3001 cgggagcgta ccggagatcc tgagacccgt gcttggggaa tacgggttcc tcaacccggt
     3061 cctcgcagcg gcggcgatgg cgctctccag cgtgaccgtg gtgagcaacg ccctcaggtt
     3121 acgcagggtg aaggttggct agtagaaagg ctaaaagaga tggacgcaac acaggacaac
     3181 gaacaggtgc cggcgcacgg ttacataaag gccagggaca aagaggtgct ccagaaccgc
     3241 ctcaggcgga tcgagggcca ggtcaggggc gtacagcgga tggtcgacga ggaggcttac
     3301 tgcgtggacg tcctcaccca gatcgccagt gtcgtcgcgg ctctggagaa ggttggcacc
     3361 atactcctca aggaccacgt cgagcactgc gtcagggagt ccatagagaa gggcgaggat
     3421 gccggcgaga agatagagga gctcaccgcc gccgtcgagc gcttcctgag ggtttaggaa
     3481 gcgtcgtcca aaaggaagga gaggtgatga ccgagaagac gttgaaggtt ccggacatga
     3541 gttgcgccca ctgcaaagcc gcggttgaag gtgaattgaa caggctctcc ggagtgcaga
     3601 gagccaacgc cgacgtcgac aagggcacgg tcgaggtcac ctacgacgag ggtaaggtga
     3661 ccactgagga cctaaagggt gccatcgagg aggccggcta cggcgtagct gcttagaagg
     3721 acagcaccac ggccgctaac tgggaccgga catcggcgtt gaaatgacat tggtcgaagc
     3781 tacggaatgg ctcgcgaaga ggagaatctc cttggagggg gtagacgaac gaccatccct
     3841 gtcaagcagt caaatggctc cgcccgtgac gagtaagaca ggaccagatc accgatgaag
     3901 ctactcggca tcaggcagct gtccgtgctc atcatcggca cgctgctctt ctgccacggg
     3961 atcttcgggg ccctgcactt gatctgctac cctccgcagt gcgccggtga cgcggatcac
     4021 gcagcggagc accaggccgc ggccggtgcg gtgggtgccg cacaccagca ccccgcggat
     4081 catggaacca gacatgggac gagcaccgag tacttcgccg tgctcgccgt cggccttctc
     4141 gggttgcttc tggggttgtt ccccaagcgt gggccgttgc ggattagcct cgccgcacat
     4201 tggcctgtgg tccttagccg gatgcccgct gtatttcgtc cttcaccaac ccccacacca
     4261 ctcactcttc aggagttcag gctctgagag gctcttcgcc ccgtaacgcg ggagcaaggg
     4321 acctaacccg aacactatac gaatgggggt tgtcttgaag acacttctga cgaaggcctg
     4381 gctgttcgcg ttgctcctgg gatgagaaca acagacccgt gccaccgggc gcctacggat
     4441 acaaggtgct tgtcacggtg ctgttctctc gcaccatgcc cctgatccgc tacgagatga
     4501 gcgacagtgt gaggctggcc tcttctcctc tctgtccctg cgggcgacct ttcgccttca
     4561 tagacgggat acagggtcga gcggaggacg tactgcgctt ccctgccaca tccggggggc
     4621 aagtctcggt gcagcccata atcttccacc gcgtgatgga caccgtgccc gccggagggt
     4681 ggcaggtggt tcagagttcc gaagggctga cagtacttct gagcggtgtg cgtgagggct
     4741 tcgcggaggc taccctgatc gactcgctgc ggcatgagct cggggcccag ggagcgatcg
     4801 tgccaccggt taaggtgagg cacgtgccga ctatcccccg caccaccgtc ggcaaggcgc
     4861 ctctcatcaa ggcccgcgtg cccgggcccc ggagaaagcc atgaacccgc caccctacca
     4921 ctcgatggtc tatggagggt aaggatgtct aggaaatggt ggttcatcct cgccgcgatg
     4981 ctgcttacgg cgacactcta ccgtaaccgg ctgcgtgcct atctcgaaca agcctccgcc
     5041 aacgtccgcg acttcgacct gcccagtgcc ggggcctacg acgcgctggt cgccaccgtc
     5101 ctggaaggcc tctacgcccg cgtcgctggc gaggtcggcg ctgctcaccc aggcggtaac
     5161 ttactggagg tcgggagtgg cccgggccgg ctcgccgtgc ggctggcgcg agaggctccc
     5221 ggcatgacgc tcaccggcgt cgatatctcg gacgtgatgg tcgagcttgc ggcccgccgc
     5281 gcggctagag ccggtctctc agagagggta cggttcgagg ttggcgacgt cgcggtgctg
     5341 ccactctccg acgaggacgt cgacggggtg gtgagcacgt ctccctgcac cactggtcgg
     5401 acccggccag cgggcgggcc gagatccacc gggtcctcaa gcccggcggg gaggcacgga
     5461 tctacgatct cgcctactgg ctgtggcgac ctgcgcggga agggaaccga ctcgcccagc
     5521 tggcagccgc gagcccgttc ggcaaggggg aagtgaaggt agtccgctgg ccgggttcca
     5581 tgccggcgtt cgtcatgctg cgactgcggc accaccctct gccgtaggaa gctataggat
     5641 gtgcaacgtg ctaaggagga tgtagccacc atgaccaaca acgaccccag ggctgtggca
     5701 acagccgatc accagcatgt gcacgtagga cgaggcacgt tcgagctccg tggcgtgtac
     5761 tgcctttgct gcgccggtgc cgttgagcgg gccttgaagg agcagccaca cgtcattgat
     5821 gttcgactgg actggaagaa cgacgtcgtc cacgtcggct acgaccccgc gaggattggc
     5881 caggaggata tcgaggacgt catcacgcag accggatgcg actgcaaccc gaccggggtc
     5941 gaggaggagc accaccacga agtcatggcg ccaccggagc ggcggatgca gcacctcggg
     6001 catggcgtgg atacgcagcc catctcgatg gggacgaagc acgaccggat gcaatacgag
     6061 atgccggcca cccatgcgga tcacggcgac catatggacc acgacatgtc agaccccctc
     6121 atggcggcaa cgatggaacg cgacatgcgc aacaaattct tcgtcgcgct gctcctcact
     6181 atcccgacag tgctctactc gccgctgggc atgaacctgc taggcgtgcg gctgcccacg
     6241 ttcgggctgg gcgagaacct gatcatgctg ctgctctcca cgcctgtcgt gttctattcg
     6301 ggttggatct tcctctccgg ctcataccat tccttacggc ggcgtacgct caacatgagc
     6361 gtgctcatag ccacgggcgt gctcgcagcc tacctgttca gcgtcctgat caccttcctc
     6421 ggcggagaga gcttctacga ggccgccgcc atgctcgtca ccttcgtcct ttttgggcac
     6481 tggatggaga tgcgctccag gcgtggcacc aacgacgccc tgcgggcgct cttcgacctg
     6541 gtgccgcccc aggcaaccgt gattcgggac ggcgaagaaa tgacaatccc gagcagcgag
     6601 gtgcagctcg gcgacacagt ggtgttgaga cctggcgaca aggtgccggt cgatggcgag
     6661 gtcatcgagg gcgagaccag catagatgag gcgctggtga cgggcgagag cgtcccggtc
     6721 acgaagcgcc cgggcgacgc cgtgatcgca ggatctataa accgcagcag cagcgtccgc
     6781 tttgaggcaa cagccgtggg cgcccacacc actctagccc agatcgtgga tctcgttcag
     6841 aaggcccaga actctaaggc gccgggacag cgtctggccg accgcgcggc ggagtacctt
     6901 gtgatcctgg ccgtcggctc gggcatcatc accttcttcg cctggtactt cctgggtggc
     6961 gctgccgcca tcacggcgat gacgtttgcg atctcggccg tggtcatcgc gtgcccggac
     7021 gccctgggcc tcgcgacccc gacagcggtg gccgttggca ccggcatcgg agcgaggcac
     7081 aacatcctga tcaaggacgc cgccaccctg gagaacgtct cacgtataga cgccatcgtc
     7141 ctggataaga ccggaaccct cacggagggc gagccctcac tcaccgacgt ttatactctg
     7201 gacggcagga gcgaggtcga cctcctgcgc ctcgttgccc cggccgagaa gaactccgaa
     7261 catccgctgg ctgaagcgat agtggcgggc gcccgcgatc gaggcatcga acccaaagat
     7321 gcttcgcgct tcgaggccgt cgccggccac ggcatccggg cggaggtgga tagccatgtt
     7381 gtgctggtgg gcaaccgcaa gctgatggac gagagtgaag tggacgtgtt cgcgctggaa
     7441 gagagggctg tcactctcgc cgaggcgggc aagacgccta tgttcgtcgc ggtcgacggg
     7501 caaccctctg ggcttgtggc agtagcggac accatcaagc cgagcgcgcc cgagacgctg
     7561 cgccgcctca agcagatggg catcgaggcg gtcatgatca cgggcgacaa caggagaacc
     7621 gccgaagccg tggcgcgcga gctcggtatc gagcgcgtct tcgccgaagt gctgcctgcc
     7681 gacaaggcac gctacgtcga ggagctccag gaagagggca agcacgtagc gatggttgga
     7741 gacggcgtca acgatgctcc tgccctcgcg caggctgaca ttggcattgc catcggcgca
     7801 ggaaccgacg tggccatcga gacagcaaag gtggttctga tgaagagcga tcccttagat
     7861 gtgatccgcg ctgtggtgct ctctaagggg accgtcagga agatgaagca gaacctggtg
     7921 tgggcttcgg tatacaacgt gctcgcgatc ccaatcgcag caggagttct cttccccgcc
     7981 tacggcatca tgctcagacc cgagtggtcg gctttgttga tgtcggtttc ctcgatcatc
     8041 gttgcggtca acgccgtgct cctcaagggg gtcgaatcgg agctcgaaga accgaaatca
     8101 gttgacggta tagtgcccga gtaaccacca tcgaacatct tctcgcaaga cagccaggag
     8161 gagtgtctgg tttcatccgt cccaagcagc aagatatagt ttggttctct agctgcggtc
     8221 acttttttgt gccaaccctg ccggcgctct cgtacccacg agctggttga tcagctccgc
     8281 agcccgccga gcaccgtccg tgggaatcgg cgggcaattc gcctcgtgcc caagcagcct
     8341 aaccattgtc tcggccagct tctcggacgt agtctcggag tagagcagcc tctggcctgc
     8401 acggtgtcaa tggagcatcg gcctctagcg gcgttgagtc tgcgtaacta tccggacaac
     8461 cagggcaacg atcagggcta tgaccagcac ccagaacagc agcacgaaca gcatgccgaa
     8521 gagtccaccg cccatcatgc caccgctcat catagacccc atgctgccca tcatgccgcc
     8581 tccacccagg acagagatga gcagtaggac aagaaccgct ccacccagta cgccgagcag
     8641 aaccttcaag ccgtcgctca tggcttataa acctccttcc tgctcttgca gtctcaactt
     8701 acagccacaa ggggatctcg gcatgaccaa gatggctcat tcacatagac aatcttgctc
     8761 atccgccgca ccgcatacct cccgcgtgcc aatggctacc cgggcgggtc gacgatcttg
     8821 aggttgagca tcattcctgt ctcgtgctcg agtatgtggc agtggaacat gtagagttgc
     8881 tcgccctcga agctgtggga gaagtccatg gcgcacttca cggtctcccc cggccaaagt
     8941 agcacggtgt ccttggagcc cacatccgtt accatcctgc ccttctcgtc caccgcgagg
     9001 tgtgcgacct gctcgggggt gccggccctt tccatcactc tgaactggaa gccgtgaagg
     9061 tgcgcaggat gaggcatgct tttctcgtcg ttgtgaatct cccagatctc tcttgcgccc
     9121 ctttggacca cgatagggaa ctcgtccatc ctgtgggtgt tgccgttgat gagccagcgc
     9181 atcccctgat cgtcggtggt catagagatc gttatgggcc gagtgtccgc cctgtccaag
     9241 tccgcttgcg ggatctcgga gagggtctcc ggcacgcttc ttgagtaatc cgtcttctct
     9301 gcaaccacca gcctcactag gtggaactcg ctgccgtctg ggagccgtgc tgggcccatc
     9361 tggtggtcca tatgatgatg gtggcccatc tccatgtgat tcatgtgctc ca
//
LOCUS       DATMJA010000302         5331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_869191, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000302 DATMJA010000000
VERSION     DATMJA010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869191"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VNT12_07250"
     CDS             <1..1045
                     /locus_tag="VNT12_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWI45953.1"
                     /translation="EMMQFHPTGMVWPPGVRGLLVTEGVRGEGGLLRNSEGVRYMENY
                     DPEKMELSTRDVVARANYTEVQEGRGSEHGGVYLDITHLGYDGIMKKLPTMYEQFLKL
                     ADIDISREPMEVFPTVHYTMGGIKIDPESAASSVPGLFAAGEVAGGLHGANRLGGNSP
                     SDLLVFGRRAGEGAAAYIEESVHSSEIDEGEVLHEIGRVLEPLEKKEEGESPYLIQQE
                     LQESMMEHANLMRDGDSLKEGLGKILALKDRLPQISVPGSRLFNPGWHTAQDVRYLVQ
                     ISEIIIRTALERKERRGAQWRLDFDGPDEELGKINFIVRKDGQGEVGIERREIPPMPE
                     HLASLVEEAKAKA"
     gene            1042..1854
                     /locus_tag="VNT12_07255"
     CDS             1042..1854
                     /locus_tag="VNT12_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HWI45954.1"
                     /translation="MSEGNGNNPGGVTSKAHSPVPNEELGTSDKMAKLRIFRGDAEGG
                     EEVEYEIPLVEGMVVLDAVLAIQAQQAPDLAVRWNCKAAHCGSCSAEINGRPKLLCKT
                     RVEEYEGAEIHVGPMRAFPVIKDLVSDVSWNYEVSKGIKPFQTSEQAPFTMYEEDVER
                     LYEPKKCIECFMCQDVCHVLRTHHKHPEFAGPRFFVRNQWLEMHPMDEADRLADLKED
                     NGLGFCNITKCCTEVCPVDIHITDNSIIPLKERVADEYYDPAKWLMRKIRGS"
     gene            complement(2076..2148)
                     /locus_tag="VNT12_07260"
     tRNA            complement(2076..2148)
                     /locus_tag="VNT12_07260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2113..2115),aa:Gly,seq:ccc)
     gene            2201..2737
                     /locus_tag="VNT12_07265"
     CDS             2201..2737
                     /locus_tag="VNT12_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI45955.1"
                     /translation="MEVEHRFSEYKFCPRCGNRLGVRLLKRGDPERLVCESCGFVFYL
                     GPKLVAGAIVELNGGIVLIQRDIEPGYGKWTFPGGFVERGERAEAAAEREVLEESGLE
                     IEVEEIVGLYTYDGQIPAIAVFAAEVTGGEPTPLDETMDIRSFPTEDLPWTEMAFPST
                     EQALKDYLRKVYSASDKS"
     gene            complement(2800..3342)
                     /locus_tag="VNT12_07270"
     CDS             complement(2800..3342)
                     /locus_tag="VNT12_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HWI45956.1"
                     /translation="MASVLVGITGGIAAYKAPGVIRRLSEAGHEVRAVATEAAFRFIP
                     EETLSVAVGSRVYTDLTWWERSGRVEHVSLARWADLVLVAPATADSMARAAIGLGDDL
                     LSATILAGAKRVLWAPAMNPEMWSSPATVRNRETLKGWGHRFVGPAEGLMASTEEKPG
                     AGRLADEAEIVAAVEEILAS"
     gene            3563..4165
                     /locus_tag="VNT12_07275"
     CDS             3563..4165
                     /locus_tag="VNT12_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI45957.1"
                     /translation="MGGKRERLGLVWIDCSYPVVAAGLKEALEGRARVHVGQTAPTDA
                     EVPCCAVFDASGAESISEGIERIRGVDPDISILVFSLHVDLPLARAALRHGARGFIHA
                     GMELDQIARAVEVAHEGEIVAPRKLLEYLIANDDPTDIDALTIRQREILQLVAQGLSN
                     AQIAKNLFLSESTVKQHLRATYKVLGVSDRKEAARLINGR"
     gene            4469..4645
                     /locus_tag="VNT12_07280"
     CDS             4469..4645
                     /locus_tag="VNT12_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45958.1"
                     /translation="MVSKTQSLRWLENLKRSQRLTAREERRKEEIMEIFRELYDLSLR
                     HQDPSIFPPWHPSW"
     gene            4694..>5331
                     /locus_tag="VNT12_07285"
     CDS             4694..>5331
                     /locus_tag="VNT12_07285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI45959.1"
                     /translation="MERALKGKVAVVTGASSGIGEATAKELARRGASVVLASRAVDKL
                     EALQRGISASGGVALAVETDVSDRGSVEAMVGRAVGELGSLDVLVNNAGLGLSGRITE
                     VLAEDVRHVFEVNAIGPLNCIQSALVHMGKGGRIINVSSVVGRRAIPKVGAYCASKAA
                     LGALSDALRVEVAGRGITVTSVYPGTTRTSFRENSRRTRDEKRGWRPKGVTAG"
BASE COUNT         1087 a         1589 c         1766 g          889 t
ORIGIN      
        1 ggagatgatg cagttccatc cgacagggat ggtctggcct ccaggcgtgc gcggtctgct
       61 cgtcaccgag ggggtgcgcg gcgagggagg actgctccgc aactccgagg gtgtgaggta
      121 catggagaac tacgacccgg agaaaatgga actctccacg cgcgacgtgg tggctcgagc
      181 caactacaca gaggtacagg agggccgcgg cagcgagcac ggcggggtct acctggatat
      241 cacgcacctc ggctacgacg ggatcatgaa gaagctgccg accatgtacg agcagtttct
      301 caaactcgcc gacatagaca tctccagaga gccgatggag gtcttcccga ccgtccacta
      361 cacgatgggt gggatcaaga tcgaccccga gagtgccgcc tccagcgtgc ccgggctctt
      421 cgccgccggc gaggtcgccg gtggtctgca cggcgcgaac cggctcgggg gcaactcgcc
      481 ctccgacttg ctcgtcttcg gccggcgtgc cggggagggc gccgcggcgt acatcgagga
      541 gagcgtgcat tcgtcggaga tagacgaggg cgaggtgctg cacgagatag ggcgtgtgct
      601 cgagccgctg gagaagaaag aagaaggcga gagcccgtat ctgatccagc aggagctcca
      661 agagtctatg atggagcatg cgaacctgat gcgcgatggg gactccctca aagagggctt
      721 gggcaagata ctcgcgctca aggaccggct gccgcagata agcgttcctg ggtccaggct
      781 cttcaaccct ggctggcaca cggcccagga cgtacgctac ctggtccaga tctcggagat
      841 catcatcagg acggccctgg agcgcaagga gcgccgcggg gcccagtgga gactagactt
      901 cgacgggcct gacgaggagc tcggcaagat caactttata gtcaggaagg acgggcaggg
      961 cgaggtaggc atcgagcggc gcgagatacc gccgatgccc gaacacctgg cgtcgctcgt
     1021 agaggaggcg aaggcgaagg catgagcgaa ggcaacggca ataatccagg gggggtgacc
     1081 tccaaggccc actccccggt accgaacgag gaacttggca ccagcgacaa gatggcgaag
     1141 ctgaggatct tccgcggcga cgcggagggc ggcgaggagg tcgagtacga gatacccctg
     1201 gtcgagggca tggtcgttct cgacgcggtg ctcgcgatac aggctcaaca ggcccccgat
     1261 cttgcggtgc gctggaactg caaggcggcc cactgcggct cgtgtagcgc cgagatcaac
     1321 ggcaggccca agctcttgtg caagacgcgc gtcgaggagt acgagggggc agagatacac
     1381 gtaggtccca tgcgggcctt ccccgtgata aaggacctgg tctccgacgt ctcctggaac
     1441 tacgaggtgt ccaagggcat caagcctttc cagaccagcg agcaggctcc cttcaccatg
     1501 tacgaggagg atgtcgagcg tctctacgaa ccgaagaagt gcatagagtg ctttatgtgc
     1561 caggacgtct gccacgtctt gaggactcac cacaaacacc ccgagttcgc aggaccacgc
     1621 ttcttcgtca ggaaccagtg gctggagatg cacccgatgg acgaagccga ccggctggcc
     1681 gacctcaagg aggacaacgg cctcgggttc tgcaacatca ccaagtgctg cacagaggtc
     1741 tgcccggtcg acatccacat caccgacaac tccatcatcc ccctcaagga gcgcgtcgcc
     1801 gacgagtact acgacccggc caaatggctg atgcgcaaga taagagggag ctagtcagca
     1861 cgctcccttc ggtcgcttgt cagcatttca gcacgctgcg ggccttcggc cctcgcttgt
     1921 caggaagtcg tccctgggag tcgccatggg cgtccgaccg catcgcgacc gacggcgact
     1981 ctccatatgc aaccgagcga ggctggaaag ggaaaaggct gacaagcgaa gcccgcaggg
     2041 tgctgaaatg ctgaagtgct gactagctac aacagagcgg acgacgggct tcgaacccgc
     2101 gacctccagc ttgggaagct ggcgctctac caactgagct acgtccgcgt aggcgggtat
     2161 tctagcacgc tgggcgtagc tctagtaacc tgagaacacc ttggaagtag agcatcggtt
     2221 ctccgagtac aagttctgcc cccggtgtgg caaccgcctc ggagtccgct tgctcaaacg
     2281 tggcgatccg gagcggctcg tgtgcgagag ttgcggcttc gtgttctacc tggggccgaa
     2341 actcgtagcg ggggctattg tcgagctgaa cggcggtata gtcctgatcc agcgcgacat
     2401 cgaaccgggc tacggcaagt ggacttttcc tggcgggttt gtcgagcgcg gtgagcgggc
     2461 cgaggccgca gcggagcgtg aggtgctcga ggagtcgggg ttggagattg aggtggagga
     2521 gatcgtcggc ctctacacgt acgacggaca gataccggcc atagccgtct tcgcagccga
     2581 ggtcacaggc ggcgaaccta cgcctctcga cgagacgatg gacatcagga gcttcccaac
     2641 agaggatctc ccgtggacgg agatggcctt cccgagcacc gaacaggccc tgaaagacta
     2701 cctgcgaaaa gtttacagcg cttctgacaa gagctgagcc atgaagtgcc agagcaggtg
     2761 tactcagcaa ggtggccatc cacttcaaat cttgatgcct cagcttgcga gaatctcttc
     2821 gacggccgcc actatctcag cttcgtcggc gagacgcccg gcgcctggtt tctcctccgt
     2881 tgacgccatg aggccttcgg cgggaccgac aaagcgatgc ccccagccct tcagcgtctc
     2941 gcggttgcgg acagtggccg ggctgctcca catctccggg ttcatggctg gagcccagag
     3001 gactctcttc gcgccggcga ggatcgttgc cgagagcaga tcatccccga gcccgatggc
     3061 ggcccgcgcc atcgagtctg cggtcgccgg ggctacgagg acgagatcgg cccagcgggc
     3121 gagggagacg tgctcgacgc ggcccgaacg ctcccaccag gtcaagtccg tgtagacgcg
     3181 ggaaccgact gccacggaga gcgtctcttc cggaataaag cggaaggcag cttctgtggc
     3241 gacggccctg acctcgtgtc cggcctcgct gaggcgccgg atcacacccg gcgccttgta
     3301 cgccgcgatg ccgcccgtta tcccgacgag tacgctggcc acttagatag tatacagagc
     3361 cggtacagac gacaacccgg cgccggacac gtgttggcgg actgcagacc aggggtagtg
     3421 cttgtgccag ggtcgatctg gatggggggc taacccgagg tgttagtacc cggacattct
     3481 ttggtaagat gcaaacagtg tcctactaca acctgatacg tgtacggcct tgcaggacct
     3541 actatagaga gaggcgacat tgatgggcgg caagcgtgag cgtttggggc ttgtctggat
     3601 agattgctcg tatcccgttg tggccgccgg gctcaaggag gcactggaag ggcgggcgcg
     3661 ggtacacgtt ggacaaaccg ctcctactga cgccgaggtt ccctgttgcg ccgtattcga
     3721 cgcgagtggc gccgaaagca tctccgaggg tatagaacgc atcagggggg tcgaccccga
     3781 catctccatt ctggtcttca gcctgcacgt ggatctgccg ctcgcccgtg ccgccctgcg
     3841 acacggagcc aggggtttca ttcacgccgg gatggagttg gatcagatag cccgcgccgt
     3901 ggaggtggcc cacgagggcg agatcgtcgc ccccaggaaa cttctcgagt acctcatagc
     3961 gaacgatgac cccacggata tcgacgcgct caccatccgc cagcgggaga tactgcagct
     4021 agtcgcccag ggactcagca acgcacagat cgccaagaac ctcttcctct ccgagtcgac
     4081 ggtaaagcaa catctgcgcg ccacctacaa ggtgctcggc gtgagcgacc gtaaggaagc
     4141 cgcgaggctc attaacggtc gctaggtttg caaagaggtt gaaaacctct ttgcaagcat
     4201 ttcatcacgc tcaggcttcg ccttcgcttc tcagcacgtc gcccgttcta ggcgacttgt
     4261 cagcatttca gcttcccggt ggcggttcgc cgcccgaaat agctgactag ctgacaagcg
     4321 aagcccgaag ggcgtagcgt gccgacaagc tggcgtagcc agcttgctga gaggcgcgaa
     4381 gcgccgtgct actatacgtg cccctctcga tggaggggca agcaccccaa gggaagaagg
     4441 ctcgccagga gcggaaggac ggaggatagt ggtcagcaag acgcagagcc tgcgctggct
     4501 ggagaacttg aagcgatcgc agaggctgac cgcccgcgag gagcggcgca aggaggagat
     4561 catggagata ttccgcgagc tctacgacct ctccctgcgg caccaggacc catctatttt
     4621 cccgccctgg catccgtcct ggtgaggggc ttcaggagaa caagacgacc tgctcctccg
     4681 agatagtctt cctgtggagc gagcactgaa aggcaaggtg gcggtcgtca ccggagcttc
     4741 gagcggcatc ggcgaggcga cggccaagga gctggcgcgg cgcggcgctt cggtcgtgct
     4801 cgcctccagg gccgtggaca aactcgaagc gctgcagcgt gggatctcag cctccggcgg
     4861 cgtggcgctg gccgtcgaga ccgacgtttc ggacaggggc tccgtcgaag cgatggtcgg
     4921 acgggccgtc ggggagctag ggtccctgga cgtactggtc aacaatgccg ggctgggact
     4981 ctcgggtagg atcaccgagg tgctggctga ggacgtgcgg cacgtgttcg aggtcaacgc
     5041 cataggcccg ctgaactgca tccagtccgc cttagtgcat atggggaagg ggggacgcat
     5101 aatcaatgtc tcgtccgtag tcggcaggcg ggctatcccc aaggtcggcg cctactgcgc
     5161 gagcaaagcc gccctgggcg ccctctccga cgcgctcagg gtggaggtcg cgggcagggg
     5221 catcaccgtc acgagcgtgt acccgggcac cacccgcacg tctttcaggg agaactccag
     5281 gcgcacgaga gatgagaagc gcggctggcg cccgaagggc gtgacagccg g
//
LOCUS       DATMJA010000303         3483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_691798, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000303 DATMJA010000000
VERSION     DATMJA010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691798"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VNT12_07290"
     CDS             complement(<1..543)
                     /locus_tag="VNT12_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45960.1"
                     /translation="MDQLTLEGVRALRDAGLSVRTYALLNVVVDKIFQLVWFVVGVLI
                     FWRRSDDRMALLVSMFLVSFGPVSVDITDAEALISSQPAWRLPVLGVEIVGNVCVVLF
                     FLLFPSGRFAPRWTRWLAAAFCAFLVSQSLFPDFYSRSLALTMVSLLVFLGIVVSLVW
                     SQTYSYHRFSSPVQRRQTKWV"
     gene            1128..1922
                     /locus_tag="VNT12_07295"
     CDS             1128..1922
                     /locus_tag="VNT12_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWI45961.1"
                     /translation="MGFSIGEVSQAVGLAPQTLRLWERERLIKPRRTDRGYRVYTEPD
                     VRRLREIKQLRKVEGLNFAAIRKHLGSAPETTSPNGPALGGPSGAPGERLRRLRIKAH
                     KTLKEVSEATGLSISFISALERGGSGASVASLRLLAGAYGANMREVFGADLEQSSPLV
                     RSAERPVMQWDNGVRFEEMASGEQVMDPSYIRVPSGAGSEGFYSHNGEEFIYVISGRL
                     FVELKDHGTFQVAPGDTLYFPSTTPHRWWAGKEPVEAVYVNTPPTF"
     gene            1929..>3483
                     /locus_tag="VNT12_07300"
     CDS             1929..>3483
                     /locus_tag="VNT12_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI45962.1"
                     /translation="MSLDLRLDTLLSIPALFGPRVSPDGKWVAWSWSRLGPAADVFAA
                     PSDGSQPPLRLTETVEGDTMVISWTSDSDAVLVSQDNDGDERVRLFRVRLADPGVMEP
                     LTAASPNYYLRGGQLHRDGRWLIYAANLDAESGEEIETDRLYRHDLQTGEMMVLARPE
                     KGSIPWPELNRQGTHVLYSRNDVHPAGQQVWVVDIDGLEDREILNFGPRIKVSASWFS
                     DGRRVLFVAEAESYRRLGVWSMDQKSVRWLVEDPTRNIEYSFVPPNGGPVVVVGVEQA
                     GMRASLLDVHNGTETSLQHTRGNLVPLAQSDNGGWICTHYDALHPVDLVRYSEEDGER
                     ASLTGLPERMDIDEERLVAAEDFRWRSVDGMEVQGWLYRTRGEKLGTIVLIHGGPTSH
                     AEDRFNAQIQYLASRGFDVLAPNYRGSTGFGLPFQESIKQDGWGGREQEDIRSGIEAL
                     IEAGTAEPERVGVTGTSYGGYSAWWAITHFDPETVAAAAPICGMTDLVVDYYATRPDL
                     RPYSEEMMGG"
BASE COUNT          753 a         1053 c         1100 g          577 t
ORIGIN      
        1 aacccacttt gtctggcggc gttgaaccgg agaggagaac ctgtggtagg aataggtctg
       61 ggaccagacc aggctcacca cgatccctag gaacaccaac aatgagacca tcgtcagggc
      121 tagcgaacga gagtaaaaat ccggaaagag actttgggaa actagaaagg cgcagaacgc
      181 agccgcgagc cagcgcgtcc agcgaggagc aaaccgtccg ctggggaaaa gcaagaaaaa
      241 cagcacgaca cagacattac ccacgatttc cacgcccagg acgggcaacc gccaggctgg
      301 ctgggaggag atcaaggctt cggcatccgt gatatcgacc gagaccggcc cgaaggacac
      361 cagaaacatc gagaccagca gcgccatccg atcatcggat cgtcgccaga agatgagcac
      421 tcccaccaca aaccacacaa gctgaaagat cttgtccacc acaacgttca gaagcgcata
      481 ggtgcgcacc gacaacccgg cgtcgcgtaa tgcccgcacg ccttccagtg taagctgatc
      541 cacggcccgc tcggaacata cctcggatgc ggcggtgcac acgctactat agtgttcgaa
      601 actgctcgga acactgaata cgactatcgc cagcgacgtg atggccacca caatccatgc
      661 cacgcgggca aataggagcc agggtccgcg cagagtagtg gaaggcggag aggcgcgtgg
      721 gctcatctca ctgattcctc gatctggcga ggatgagtgt gggccccggc cgcgtaggca
      781 aagcggaacg ccatcctggc gccaggctgg cctgtcgtac tcccgactat cttctcactc
      841 cttccgagtt gcccggaggt tctctttgtc tcaccaccat agcggacggg cgtctgccaa
      901 gcgttacgaa gagcgttacg atcgagaatt tctgaaaacc acgcgagatt gccttcgcat
      961 tgcagccctc gactcgcagt gcgacgacag cccttcttca cccggtgggc cctaccgaaa
     1021 ggtggatgct tgcttttatg gagaaacctt tacaataagg gttcaagcat acagggactg
     1081 gcattgcggg cacgacggga gatgcattgg cacagggaag cggttcgatg gggttttcca
     1141 taggcgaggt ttcacaggcg gtagggcttg cgccgcagac cttgaggttg tgggaaaggg
     1201 aacgcctgat caaaccccgc aggacagacc ggggatacag ggtgtacacg gaacccgacg
     1261 taaggcggct gcgcgagatc aaacagctca ggaaggtcga gggcctgaac ttcgcggcga
     1321 tacgcaagca cctgggcagc gcacctgaga cgacaagccc caacggaccg gcgctcggcg
     1381 gaccgtcagg ggcgcctggg gagcgcttgc ggagactcag gatcaaggcc cacaagaccc
     1441 tgaaggaggt ctcggaggcc actgggctct ctatctcgtt tatctccgcg ctggagcggg
     1501 gaggttcggg agcttcggtc gcgtctttgc ggctgctcgc gggggcctac ggggcgaaca
     1561 tgcgcgaggt gttcggcgcc gacctcgagc agagctcgcc gctggtgcgg agcgccgaaa
     1621 ggcccgtgat gcagtgggat aacggcgtgc gattcgagga gatggcctcg ggtgagcagg
     1681 tcatggaccc ttcctacata agggtgcctt ccggcgcggg gagcgagggt ttctactccc
     1741 acaacggaga agagttcatc tacgtgatct ccggccggct gttcgtggaa ctaaaagacc
     1801 atgggacgtt ccaggtcgcc cctggggaca cgctctattt cccctcgacc accccgcatc
     1861 gctggtgggc cggcaaagag cccgtcgagg ccgtctacgt caacaccccg ccgacgtttt
     1921 agccagtggt gtccctagat ctcaggctcg acaccctgct ctccatccct gcgctcttcg
     1981 ggccgcgcgt ctcacccgac gggaaatggg tggcttggag ctggtcgcgc ctcgggcctg
     2041 ccgccgacgt gttcgcagca ccatccgacg gctcgcagcc gcccctccgc ctcactgaga
     2101 ccgtagaggg ggacacaatg gtcatctcct ggacctcgga cagcgacgcg gtcctcgtat
     2161 cgcaagacaa cgacggagat gagcgcgtac ggctcttccg ggtcagactc gctgatccag
     2221 gggtgatgga gccactcacc gcggcgagcc cgaactacta tctcaggggc ggccagttgc
     2281 atagagacgg ccgctggctc atctacgctg ccaacctcga cgccgagagc ggagaggaga
     2341 tcgaaaccga ccgactctac aggcacgatt tacagacggg agaaatgatg gtgctggcca
     2401 gacccgagaa ggggagtatt ccctggccgg agctgaaccg tcagggtacc cacgtactct
     2461 actcgcgcaa cgatgtgcac ccggccggac agcaagtctg ggtggtcgac atagatggcc
     2521 tcgaagatcg cgagatcctg aacttcggcc cgcggatcaa ggtctctgca tcctggttct
     2581 ccgacgggcg cagggtcctc ttcgtcgccg aggctgaatc ttatcggagg ctgggtgtat
     2641 ggagcatgga ccaaaagtct gtgcgctggc tcgtagaaga tccaacccgc aacatcgagt
     2701 actccttcgt gccgccgaac gggggacctg tagtcgtggt gggggtcgag caagccggga
     2761 tgagggcctc gctgctcgac gtgcataacg gcaccgagac ctcactacag cacacaagag
     2821 gcaaccttgt accgctggcc caatccgata atggcggttg gatctgtacc cactacgatg
     2881 ccctccaccc tgtggacctc gtgcgctaca gcgaggagga tggcgaacgg gccagcctca
     2941 cgggcctgcc ggaaaggatg gacatagacg aggagcggct cgtcgccgcc gaagacttcc
     3001 gctggcggtc tgtcgacggc atggaggttc agggttggct ctacagaaca cgtggcgaga
     3061 aacttggaac catcgtcttg attcacggcg gcccgacaag ccacgccgaa gaccggttca
     3121 acgcccagat ccagtacctt gcctcgcgcg gcttcgacgt gctggcgccg aactacaggg
     3181 gcagcacggg gttcgggtta cctttccagg agtccataaa acaggacggc tggggcggcc
     3241 gggagcagga agatataagg agtgggatcg aggcgctcat cgaggccggc accgcagagc
     3301 cggaacgcgt tggcgttacc ggcacctctt acggcggata ctcggcgtgg tgggcgatca
     3361 cccacttcga ccccgagacg gtcgccgccg cggcaccgat ctgcggcatg accgacctcg
     3421 tggtcgacta ctacgccaca cgccccgacc tcaggcccta cagcgaagag atgatgggcg
     3481 gct
//
LOCUS       DATMJA010000304         2552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_336778, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000304 DATMJA010000000
VERSION     DATMJA010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336778"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /gene="mce"
                     /locus_tag="VNT12_07305"
     CDS             <1..389
                     /gene="mce"
                     /locus_tag="VNT12_07305"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWI45963.1"
                     /translation="LGYAVEDIEAASRFYEENFGAVPGEPEVIEEQGIIAIMFSVGES
                     SIELVQPTRPDSPVGRFLQKRGEGFHHVAFVVEDLSGTLAELKRNGVEIIDEEPRRGA
                     GGSRMVFVHPRGAFGVLTELVELPHA"
     gene            410..2008
                     /locus_tag="VNT12_07310"
     CDS             410..2008
                     /locus_tag="VNT12_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWI45964.1"
                     /translation="MDTEKRQDERRTESGIEVKPLYKPEDLEGFDYPERLGDPGAYPY
                     TRGPYPSMYRGRPWTMRQYAGFGTARETNARFKYLIDNGQTGLSVAFDLPTQMGRDSD
                     HALALGEVGKVGVAIDSLLDMRDLFEGIPLSEVSTSMTINATASILLLLYSLVAEEQG
                     VEADGVTGTTQNDILKEYLARGTYIYPPGPSMRITTDMFAYCAAESPRWNTISISGYH
                     IREAGSTAVQELAFTLSNAIAYVEAALDAGLEVDEFAPRLSFFFNAHNDLFQEVAKYR
                     AARRMWAKIMRDRFGASDERSRKLRFHTQTAGSSLTAQQAENNIVRTTVQALSAVLGG
                     TQSLHTNAFDEALALPTERSARIALRTQQILASEGGLTGTADPLAGSYYVESLTDALE
                     EAGWAYIRRIDELGGSVKAVEKRFMQREIEDAAYRYQREVEREDRVIVGVNRFTSGGT
                     DDPDMELHTVDETIRERQKERLSELKVSRDNAAVEKALASLKGAAEGSENLLYPMKDA
                     LAELATLGEVSDTLRDVFGEYRPS"
     gene            2198..>2552
                     /locus_tag="VNT12_07315"
     CDS             2198..>2552
                     /locus_tag="VNT12_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HWI45965.1"
                     /translation="MVDWFRRRKAGPPDDVGRAAHVGAEAAEELVDRTSEPADGFSEA
                     GIREALRDVRDPEIGRDLVSLNMVRSIDIAGSHVTVGVSLTTAGCPLKHRITTDIRDR
                     LKMIEGIEEVEVDFGV"
BASE COUNT          526 a          763 c          839 g          424 t
ORIGIN      
        1 atctcggtta cgcggtggag gacatcgagg ccgcctcgag gttctatgag gagaacttcg
       61 gggccgtgcc cggagagcct gaggttattg aggagcaggg cataatcgcc atcatgttca
      121 gtgtcgggga atcgagcatc gaactcgtcc agcccaccag gccggactca cctgtcggga
      181 ggtttctgca gaaacgcggc gagggcttcc accacgtcgc tttcgtggta gaggatctca
      241 gcgggacgct ggcggagctc aagcgtaacg gcgtcgagat catagacgag gaaccgagga
      301 ggggggcagg ggggagcaga atggtcttcg tccaccccag gggtgccttc ggggtactca
      361 ccgagctagt agaattgcca cacgcctgag tcgagcgggg aggaggagcg tggacaccga
      421 gaaacgccag gacgagcgcc gtacggagtc cgggatcgag gtcaagcccc tctacaagcc
      481 agaggacctc gaaggcttcg actaccccga acgccttggt gacccaggag cgtaccccta
      541 cacccgtggt ccctacccga gcatgtatcg cggcaggcca tggaccatgc gacaatacgc
      601 ggggttcgga acggcgcgcg agacgaacgc ccgcttcaag tacctcatag acaacggcca
      661 gaccgggctg tccgtcgcct tcgacctgcc cacgcagatg gggagggact ccgaccatgc
      721 gctcgccctc ggggaggtgg gcaaggtcgg cgtggctatc gactcgcttt tggacatgcg
      781 cgacctcttc gaaggaatcc ccttgagcga ggtctcgact tcgatgacga taaacgcgac
      841 ggcctcgatc ctgttgctcc tctactcgct agtcgccgaa gagcagggtg tggaggctga
      901 tggcgtcacg gggaccaccc agaatgacat actcaaggag tatctggccc gaggcaccta
      961 catctacccg cctgggcctt cgatgaggat caccaccgac atgttcgcct actgtgcagc
     1021 agagtcgccg cgctggaaca ccatctcgat cagcggatac cacataaggg aggctgggtc
     1081 caccgctgtc caggagctcg ctttcaccct ctccaacgcc atagcctacg tcgaggctgc
     1141 cctcgacgcc gggcttgagg tggacgagtt cgcgcccagg ctctcgttct tcttcaacgc
     1201 ccacaacgac ctgttccagg aggtggccaa gtacagggcg gcgaggagga tgtgggcaaa
     1261 gatcatgcgc gaccgcttcg gcgcaagcga cgagaggagc agaaagctaa ggttccacac
     1321 ccagaccgcg ggctcctcgc tgacggccca gcaggccgag aacaacatcg tccgcaccac
     1381 ggtccaggcg ctctccgcgg tcctcggcgg gacccagagc ctgcacacca acgccttcga
     1441 cgaggccctc gcgctaccga cggagcggag cgcgcgtatc gccctcagaa cccagcagat
     1501 actggcgagt gagggcggcc tgacagggac ggcagacccg cttgccggct cctattacgt
     1561 cgagtcgctc acggacgcgc tcgaggaggc aggctgggcc tacatacggc gcatcgacga
     1621 gctgggaggc tcggtaaagg ccgtcgagaa gcgctttatg cagcgtgaga tcgaggacgc
     1681 cgcctaccgc tatcagcggg aggtggagag agaggatcgt gtcatcgtcg gcgtcaaccg
     1741 cttcacctcg ggtggaaccg acgacccgga tatggagctc cacaccgtcg acgagaccat
     1801 ccgcgaacgg cagaaggaaa ggctctccga gctcaaagtg agcagggata acgctgccgt
     1861 cgagaaggcc ctcgccagct tgaagggcgc ggccgagggt tccgagaacc tcctttaccc
     1921 gatgaaagat gccctggcag agctcgcgac cctaggcgag gtctccgaca ccctccgcga
     1981 tgtcttcgga gagtacaggc cttcttgaaa gcggtgtgcg gaaggattgg atcggggcca
     2041 ggatctacgc cccgagcgct tcgcgtagag gggccgaaag gcacgtgcgg cgctgtgatt
     2101 gacctgtgtt aatattggcc aaaagcagga agttcctacc gttctttcga gctagtttag
     2161 aatcaccggt ccgtggcgcc ggaggaggtt agaagacgtg gttgactggt ttcgcagacg
     2221 taaggccggg ccgcccgacg atgttgggcg ggcggcgcat gtcggggccg aggcggcaga
     2281 agagctcgtg gaccgtactt ccgagccggc cgacgggttc tctgaggccg gtattcgtga
     2341 ggctttgagg gacgtaaggg accctgagat cgggcgcgac ctcgtctcgc tgaacatggt
     2401 ccgctccatc gacatagcgg gttcgcatgt cacggtaggc gtctccctca cgaccgcggg
     2461 ctgccctttg aagcacagga tcaccactga cataagggac cgcctcaaga tgatcgaagg
     2521 catagaagaa gtcgaggtcg acttcggcgt ta
//
LOCUS       DATMJA010000305         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_159260, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000305 DATMJA010000000
VERSION     DATMJA010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159260"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..408
                     /locus_tag="VNT12_07320"
     CDS             217..408
                     /locus_tag="VNT12_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45966.1"
                     /translation="MPKEPQDRSADVSVQQVGPGEEPARTTPEEVAEPGRKRTAADTE
                     WLSMATADPAHFLKDLHAE"
     gene            complement(638..907)
                     /locus_tag="VNT12_07325"
     CDS             complement(638..907)
                     /locus_tag="VNT12_07325"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HWI45967.1"
                     /translation="MDENRLDGNAAAGVLAEIFTFEVTTALAACARCGAAGEIGSQMA
                     YVSEIGTVVRCATCDNALIRVARQDDGPQRYWLDLKGIEYLQLGS"
     gene            complement(910..1656)
                     /locus_tag="VNT12_07330"
     CDS             complement(910..1656)
                     /locus_tag="VNT12_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HWI45968.1"
                     /translation="MASVPRKLAWRVGEVVETSPDTPRTKSLFLEVPGWEGHRAGQHV
                     DVRLTAEDGYQAQRSYSIASAPEDGKVELLVERLDDGEVSPYLTDELRAGDGLELRGP
                     IGGWFAWDASEGGPLLLVAGGSGIAPLMAMIRHRQAAGNDTPVRLLYSSRSYEDVMFR
                     EELERLAAEDETLEITHTLTRSAPPGWKGYDRRIDEEMLGKVAFSPDERPLAFVCGPT
                     PLVEAVATDLVGLGHEPARVKTERFGPTGG"
     gene            complement(1658..2269)
                     /locus_tag="VNT12_07335"
     CDS             complement(1658..2269)
                     /locus_tag="VNT12_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HWI45969.1"
                     /translation="MSGGFISRGFRGRRRRAEGDDRVPPGQYLVEGFPVLSAGPTPRT
                     PLEEWDFTIEGEVDGPRTWSWEEFRALPSEEITRDIHCVTKWSKLGTTWEGVSVDTLL
                     EGVDTAAVYVVAFSDGGYTTNLPLEEVTGGKAWVAHTYEGEPLAPEHGGPARLLVPHL
                     YFWKSAKWVRGLRLVAEDEPGFWESLGYHNHGDPWREQRYWGD"
BASE COUNT          474 a          875 c          791 g          453 t
ORIGIN      
        1 tcgacgaggt cgcccagatc gtcgccggag gcgccgcctc caccacggcc ctcaccggct
       61 cgacagagga agcccagttc gacagtccgg agagccccgt cacaggcctc cccggctcca
      121 ccgagaacga gcaattcgtg aagtaggtgt caggcttcag gttacaggca tcaggggagc
      181 cgggaacccc ggctccctct agcaaaggag gagaccatgc ctaaagagcc acaggaccgc
      241 agtgcggacg ttagcgtgca gcaggtcggg cccggggaag agccggcccg tacaacgccc
      301 gaagaggttg cggagcctgg ccggaagcgg accgcagcgg acacagagtg gctatctatg
      361 gcaaccgcag acccggccca tttcctcaaa gacctacacg cggagtaata ccgagtccac
      421 tcgaaggaga gcgcgttcga gctctgctgg cacgccgagg cctctcccat acgtcattcc
      481 cgcgcaagcg ggaatccact gtgacgcagg tgttcagtac ctccaggacc gatccaccca
      541 atcatctttg aaaagctgaa ccgctgacga gcgaaggcga agcctgagcg tgccgaaatg
      601 cttttaagcc gcctggcgaa gccaggcggc gtgctgacta gctaccaagc tgcaggtact
      661 ctatgccctt gagatccaac cagtaacgct gcggtccgtc gtcctggcgg gcgacgcgga
      721 tcagggcgtt gtcgcacgtg gcgcagcgca ccaccgtacc tatctcgctc acgtaagcca
      781 tctgggagcc gatctctccc gccgcgccgc aacgggcgca ggcggcgagg gccgtggtga
      841 cctcgaaggt gaagatctcg gccagtacgc ctgccgcagc gttgccgtcg agcctgtttt
      901 cgtccatcgc tatcctccag tcggtccgaa gcgttcggtc ttgacgcgtg ctggctcgtg
      961 gcctagcccg acgagatccg tcgcgacagc ctcgaccagc ggggtaggac cacacacgaa
     1021 ggccagaggc ctctcatctg gggagaaggc gaccttcccg agcatctcct cgtcgatcct
     1081 gcggtcataa cccttccagc ctggcggcgc cgagcgggtg agtgtgtggg ttatctcaag
     1141 cgtctcgtcc tcggccgcga gccgctccag ctcttcgcgg aacatgacat cctcatacga
     1201 acgggaggag tagagcaggc ggacgggggt gtcgtttccc gcggcctggc ggtgacggat
     1261 catcgccatg agcggcgcta tgcccgatcc tccggcgacg aggagcaagg gtcctccctc
     1321 actggcatcc caggcgaacc agcctccgat cggaccccgc aactccaacc cgtcgccggc
     1381 cctgagctca tcagtcaggt agggcgagac ctcaccatcg tccagccgct cgaccaggag
     1441 ctcgaccttg ccgtcttccg gcgccgaggc tatggagtag ctgcgctgag cctggtagcc
     1501 gtcctcggcg gtaagcctga cgtcgacgtg ctggcccgct ctgtgcccct cccagcccgg
     1561 tacttccagg aacagactct tcgttcgcgg cgtgtcgggg ctagtctcga ctacctcgcc
     1621 gacacgccag gcgagcttgc gcgggacgct ggccatctca gtctccccag tagcgctgct
     1681 cgcgccacgg gtcgccatgg ttgtggtagc cgagggactc ccagaagcca ggctcgtcct
     1741 cggccacgag cctgaggccc cttacccact tggcgctctt ccagaagtag aggtgcggga
     1801 cgagaaggcg ggcggggccg ccatgctcag gcgcgagtgg ttctccttcg taggtgtggg
     1861 cgacccaggc cttgcccccc gtgacctctt caagaggaag gttcgtcgtg tagcccccgt
     1921 ctgagaaagc gaccacgtac accgccgccg tatccactcc ttctagcaat gtgtccacgg
     1981 agacgccctc ccaggtcgtc cctagcttcg accacttggt gacgcagtgg atgtccctgg
     2041 tgatctcctc cgagggcaac gcgcggaact cttcccacga ccacgtcctc ggtccgtcga
     2101 cctcgccctc gatcgtaaag tcccactcct ccagaggtgt gcgtggcgtc gggcctgcag
     2161 agaggacggg gaagccctcc accaggtact ggcccggcgg gacccggtcg tctccctcag
     2221 cccgcctgcg ccggcctcga aagccgcgag agatgaatcc tccactcacg aatgtacctc
     2281 ctgccgtcgt actccgccga gaatacatcc attcttgcac gccgccgaag gcagggtcaa
     2341 acgtgcgtac ctgacgggtc tccgtgccgg aggtcccgca cgttcgaagc agttcgcgta
     2401 cccgctatac gctgggaaac actcaatggc tggatgcgta cagaagatgg gtgccctgat
     2461 atctgatagt cgtcttgcaa tccgagtggt ggctggcgct gcgatgctca tagccatact
     2521 accacggagg acgccgtccc agaattgtct cgagcaactc ttcagctgtg cgttgcaatt
     2581 ggatgcctgt atc
//
LOCUS       DATMJA010000306         4369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_395896, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000306 DATMJA010000000
VERSION     DATMJA010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395896"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VNT12_07340"
     CDS             <1..681
                     /locus_tag="VNT12_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HWI45970.1"
                     /translation="SIMQSDRLGQVRTGAASGVATKHLAREDANTLGIYGAGWQAESQ
                     LEAVAAVRDLDRVIVYSRSEGSRKEFAEKMGGRLGMEIETTHAAEEPAAQDIVVTVTS
                     SREPVLLGEWLRPGAHINAAGSNFLFKSEIDGEVVRRASFVCADSREELGLEAGDLMQ
                     SLETGAILPEAVYELGQVIAGQVPGRRGPEDITLFASQGLALEDMAAARVVYDRAIER
                     DVGRDIAF"
     gene            complement(834..959)
                     /locus_tag="VNT12_07345"
     CDS             complement(834..959)
                     /locus_tag="VNT12_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45971.1"
                     /translation="MYAAVETAARAGPVLDHDGLSVHRDVQPTVRHPEKHEGWHE"
     gene            1226..3658
                     /locus_tag="VNT12_07350"
     CDS             1226..3658
                     /locus_tag="VNT12_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HWI45972.1"
                     /translation="MSETFEKQPETGGDYKPLGAAELLDHDDRGLRVRAGSTTVEVAA
                     LAPDLFRVGMFPAGGPPRYDSEGVAKVDWGTVEVSMQESDEELTLSTTAATARISLNP
                     LRIGFTDPSGRKFAVDDEELGMSTVETPEADVFSEPLGCPVRLYKRREAGERYFGCGE
                     RTSGLEKTGSHQVFYNVDPPLGHTASFNNLYSSIPFTFSITNGKAHGLFFDNTHRVEF
                     DLALEDENRAYYGAEGGAIVYYVFCGPTPREVVDRYTELTGRTPMPPLWTLGNQQCRY
                     SYMNEEEVREVARGFRERDIPCDVIYLDIHYMDGYRVFTWNEDRFPDPRRLISDLGEQ
                     GFRVVTIVDPGVKVDENYFVYTEGRERDLYCKTREGEEYHNAVWPGVCAFPDFTNPET
                     REWWGEKQRVLTDVGVAGIWCDMNEPALFIPDGATMPDDVVHPGGRAGEARWHAQIHN
                     TYGSLMARAAREGLLALRPDERPFVITRAGYAGLQRHAMQWTGDNSSWWEHLWMSMPQ
                     LQNLALSGVAWAGVDVGGFGGDTNGELLARWTEFGIFQPYCRNHTTIGTRRQEPWVFG
                     EPYESVCREMIKLRQRLLPYLYSLFDECHRTGAPILRPLLFEYPADEMTYTADDEFLL
                     GDALLVAPITRPGIEHRHVYLPEGTWFHYYSGEQFEGPVHILAHAPLGEPALYVKGNS
                     AIPMGPDAAHTGERPNDPLTLLVYPAKGKGESTIYEDAGNGFAYEEGEYARRWISCES
                     SDDRIILRLGERQGSFVPERREVRLELRGITTAQGVTVNDEEHAPDRVEGDALTVALG
                     EEAAPTTVEVIL"
     gene            3686..>4369
                     /locus_tag="VNT12_07355"
     CDS             3686..>4369
                     /locus_tag="VNT12_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI45973.1"
                     /translation="MLGRMLVILIILSVGYFGVGLLVVLWMTSPSRRSPEATPASVGL
                     GYREVEFLSTDGIRLSAWWVPVEGSSLAAVLVPGWGGYKFDEHLLQTLPVYQDAGYSV
                     LLLDLRAQGESDGARRTLGYREVRDVHGALAWLQRQGYALDQVVLHGWSMGGATALRA
                     APGTGVAAVIEEAGYADLPILLKGEIPEFVRFGRPLRPAILLAGRLFPDFDPWDVVPK
                     KDAAKLSDEG"
BASE COUNT          812 a         1381 c         1442 g          734 t
ORIGIN      
        1 tctatcatgc agtcagacag gctcggacag gtgcgcacag gcgcggcgag cggggtggcg
       61 accaagcacc tcgcccgcga ggacgcgaac actctgggca tctatggggc tggctggcaa
      121 gccgagagcc agctagaggc cgtagcggcc gtcagggacc tggaccgggt aatcgtctac
      181 agccgcagcg aggggtcgcg caaggagttt gcggagaaga tgggcgggcg gctcgggatg
      241 gagatagaaa cgacccacgc ggccgaggag cctgccgccc aggacatcgt cgtcaccgtg
      301 acatcatcga gggagcctgt actcctcggc gagtggctca gaccaggggc gcacatcaac
      361 gccgcagggt ccaacttcct gttcaagtcc gagatcgatg gcgaggtcgt aaggcgggcg
      421 agcttcgtat gcgccgactc gcgtgaggag ctcggtctcg aggcaggaga cttgatgcaa
      481 tccctcgaaa ctggcgccat ccttcccgag gccgtctacg agctcggcca ggtcatcgcc
      541 ggacaggtgc ctggccgccg cggccccgag gacataaccc tcttcgcaag ccagggtctc
      601 gccctggaag acatggccgc agcccgtgtc gtctacgacc gcgcaatcga gcgcgacgtg
      661 ggcagggata ttgcgtttta ggacgagacc cctgggagtg gtgcatgtat ggggctcgta
      721 agggcgcttc gcgaagcgcc cttacccgaa ccgcccctat ggtcctcgtc gaaggtgcgg
      781 ttgtgagggg cactaccgca catcacaggc cggcgctggt gccgaacctg ttcttactcg
      841 tgccagccct catgcttctc ggggtggcga accgtgggct ggacgtctcg atgaacgctc
      901 aggccgtcgt ggtcgagaac agggccagcc ctcgccgccg tctccaccgc agcgtacaca
      961 gacttcctcg tcggcccgcc ctttatcggg ttcctcgccg aactgacagg ccctggctac
     1021 gccctctacc tcgtcgtagt cctcagcgcc gccatagtag tcttcgccgg agccgtaaag
     1081 acaggaggca gcaaggcaaa gtagtagatt agaatgccag cctcaaccaa aaagctgaaa
     1141 tgctgacaag cgcccgacag ggcgcgtgct gactagccgc ctgcaaggcg gcgtgctgac
     1201 cagcttctga ccgcagggag gaacattgtc cgagaccttc gagaagcagc ccgagacagg
     1261 gggcgactac aagccgctcg gcgccgcgga gttgctcgac catgacgaca ggggcctgcg
     1321 ggtgagggcc gggtcgacga ccgtcgaagt agcagcactg gcccccgacc tgttccgggt
     1381 cgggatgttc cctgcgggcg gcccgccgag gtacgactcg gagggcgttg ccaaagtgga
     1441 ctggggcact gtggaggtct ctatgcagga atccgacgag gagctcacgc tctcgacgac
     1501 agcggcgacg gcccgcatct cgttgaaccc cctgcgcatc gggttcaccg atccttccgg
     1561 caggaagttc gccgtcgacg acgaagagct cgggatgagc accgtcgaga cccccgaggc
     1621 cgacgtcttc tcggaaccct tgggctgtcc ggtacggctg tacaagaggc gagaggcagg
     1681 agaacggtac ttcgggtgcg gcgagcggac gagcgggctg gagaagacgg gctctcacca
     1741 ggtcttctac aacgtcgacc cgccgctggg gcacaccgca tccttcaaca acctctacag
     1801 ctccatcccg ttcaccttct cgattacgaa cggcaaggcc cacggcctct tcttcgacaa
     1861 cacccacaga gtcgagttcg acctcgcgct ggaagatgag aaccgcgcct actacggggc
     1921 tgagggtggg gccatcgtgt attacgtctt ctgtgggccg accccaaggg aggtcgtcga
     1981 ccgctacacc gagctcacgg gccgtactcc gatgcctccg ctgtggacct tggggaacca
     2041 gcagtgccgc tactcctaca tgaacgaaga agaagtgcgc gaggtcgcgc gtggttttcg
     2101 ggagcgggac atcccgtgcg atgtgatcta cctcgacatc cattacatgg acggctaccg
     2161 cgtcttcacc tggaacgagg atcggtttcc cgacccgcgg cgcctgatct ccgatctggg
     2221 agagcagggc ttccgggtcg tcaccatcgt cgatcccggt gtgaaggtgg acgagaacta
     2281 ctttgtatac accgagggac gggagaggga tctgtactgc aagacgcgcg agggcgagga
     2341 gtaccacaac gccgtctggc ccggcgtgtg cgccttcccc gacttcacca accccgagac
     2401 gcgcgaatgg tggggcgaga agcagagggt tcttaccgat gtcggggtgg ccgggatctg
     2461 gtgcgacatg aacgagccgg cgctcttcat accagacggg gccaccatgc ccgacgacgt
     2521 ggtccatccc ggcgggcggg ccggcgaggc caggtggcac gcccagatcc acaacaccta
     2581 cggctccctg atggcccgcg ccgccagaga gggccttctg gccctgcggc ccgacgaacg
     2641 gccgttcgtg atcacccgcg ccggctacgc tggcctgcag cgccacgcca tgcaatggac
     2701 cggggacaac tccagctggt gggagcacct gtggatgagc atgccacaac tccagaatct
     2761 cgccctctca ggcgtcgcct gggccggggt cgacgtggga ggattcgggg gcgacactaa
     2821 cggagagctc ctggcccgct ggacagaatt cgggatcttc cagccttact gccgcaacca
     2881 cactaccata gggacccgcc gccaggagcc atgggtcttc ggcgagccct acgagtcggt
     2941 gtgcagggag atgatcaaac ttcgccagcg actcctcccc tacctctact cgctcttcga
     3001 cgagtgccac aggacaggcg cgccgatcct gcgccccctg ctgttcgagt atcccgccga
     3061 cgagatgacc tacacggccg acgacgagtt cttgctcggc gacgccctgc tcgtcgcccc
     3121 cataacccgt cccggtatcg agcaccgcca cgtctacctg cccgaaggca cctggttcca
     3181 ctactacagc ggcgagcagt tcgaagggcc ggtccacatc ctggcgcacg cgcccctggg
     3241 cgagcccgcc ctctacgtaa agggaaattc tgccattccg atggggcccg acgcggccca
     3301 cacgggcgag aggccgaacg acccgctgac gctgctggtc tacccggcca agggtaaagg
     3361 agaatccaca atctacgagg atgcggggaa cggcttcgct tacgaagaag gtgagtacgc
     3421 caggcgttgg atctcctgcg agtcctcgga cgaccgaatc atcctccgcc tcggtgaacg
     3481 ccagggctcg ttcgtacccg aacgcaggga ggtacgtctt gagttgcgag gcatcacaac
     3541 ggcgcagggt gtaaccgtca acgatgagga gcacgcgcca gaccgagtag agggtgatgc
     3601 cctaaccgta gcgctcggcg aggaggccgc tccgacgacg gtggaggtga tcctctagct
     3661 gccgggcgct aggaggatgc gaacgatgct aggccgaatg ctcgtcatcc tcatcattct
     3721 gtcggtgggg tacttcggcg tgggattgct agtggtcctg tggatgacct ccccgagccg
     3781 cagatccccg gaggcgacgc ctgcgtccgt cggtcttgga tacagggagg tcgagttcct
     3841 gagcaccgat gggatccgac tcagcgcctg gtgggtgcct gttgagggtt cgtcactggc
     3901 agcggtactc gtccctggct ggggcggata caagttcgac gagcacctgc tgcagaccct
     3961 tcctgtctac caggacgccg gctacagtgt gctgctgttg gatctgcggg cgcaggggga
     4021 atcagacggg gcgcgccgca ccctcggtta cagggaagtt cgcgacgtgc acggtgcgct
     4081 cgcctggtta cagcggcaag ggtatgcgct ggatcaggtg gtgctccacg gctggtctat
     4141 gggaggagcg acagccttgc gcgcagcccc ggggacgggt gtcgccgcag tcatcgaaga
     4201 ggccgggtac gcggatcttc cgatccttct gaaaggcgag attcctgagt tcgtgcgctt
     4261 cggaaggccc cttcggccgg cgatcctgct cgcagggagg ctcttccccg atttcgaccc
     4321 ctgggacgtg gtaccgaaga aggatgccgc gaagctctcg gatgagggt
//
LOCUS       DATMJA010000307         6215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_455192, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000307 DATMJA010000000
VERSION     DATMJA010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455192"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VNT12_07360"
     CDS             <1..500
                     /locus_tag="VNT12_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWI45974.1"
                     /translation="AIRGTLLGSLILCVFAIIGDVLLEALGIGLPAFRIAGGMLLFLL
                     SLDMVFARPSGMRSATVQEQEEEEYQQDISVFPLAIPLLAGPGGITTVLLFTGGSSAG
                     QVALFMVVLLVVLLLTLGSLLLAPRVMRLLGETGANVLSRVLGVLLAALAVQFVIDGL
                     QSSLA"
     gene            522..1268
                     /locus_tag="VNT12_07365"
     CDS             522..1268
                     /locus_tag="VNT12_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45975.1"
                     /translation="MSGAPIVIVGGATWWPGSYRNLASMLQEISGSEAYIVPVTPLDW
                     TLGYFRGFGQLVFEVASAVDRALLESEAKKAVLVGHSAGGLACRVYIGGDPPYGGRRY
                     SGHRRVESLITLGTPHTVANRQRLAPITRVNELFPGAFHAGAGLRYVSVAGSVADGGS
                     SPRVRKRYERFVEDGRVAGDGVVPVDAAVLPGSESLIVDGIHHNRRLGRWYGSDTETV
                     GRWWPQELRAGGSLVADTRARLARRGMDAT"
     gene            1258..1884
                     /locus_tag="VNT12_07370"
     CDS             1258..1884
                     /locus_tag="VNT12_07370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWI45976.1"
                     /translation="MPPDGTDRREYPWKIREAEADALRSAMSHYPTGVTVVTSGREEK
                     VEGMTANAVISVSLDPLLFLVSVHKDARINQRIREEGSFTVNLLADDQEGLSRLFASP
                     ERSSGLPALHSLGGGYSSVGAPLAAGALAAIDCELAEIYYGGDHDLFLGRVVEVRLGD
                     TRKGPLIFHEGDYPALKAAPRPEESGAFVDSVRGFDARLNRLRDRRRR"
     gene            1885..2712
                     /locus_tag="VNT12_07375"
     CDS             1885..2712
                     /locus_tag="VNT12_07375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI45977.1"
                     /translation="MIRKNLLSIVCPRPSDTGGLVYAGGIERDGMILAGETRSAATGE
                     TYKIRDGYLDLLKARTGADSLANLTNFLPGAGRGYEPLWRLHSLSFLTGEPFPNEREL
                     EIIKRLVRPVRGGRYLDLGCSAGLYTRSLSGNLDSGVAVGIDISPSMLREAARRARGI
                     GANPSFVRANAKHLPFLDTSFAGAVCGGSLNEFGDPARVLRETYRILEPGGRLAIMGI
                     LRAGTPRGRRLQRILSTGGIRFFDPDELQSLLDHAGFEPDPPQTYGPVFFSGSTRRG"
     gene            complement(2728..3633)
                     /gene="rbsK"
                     /locus_tag="VNT12_07380"
     CDS             complement(2728..3633)
                     /gene="rbsK"
                     /locus_tag="VNT12_07380"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HWI45978.1"
                     /translation="MADVFVVGSINQDFVLIVERRPAPGETVTDARLATHNGGKGANQ
                     AAAAALLGASVAFLGRVGDDGFGGPLVRSLADKGVDTTLVQEVPGSSTGAAFITVTPD
                     GENAITVAPGANRCLTAEDVDDASGSIGEARVLVVQMEVPSEVVLRAVEVAAGSGTRA
                     LVNLAPPFEVPRTLLEKLDPLVINEHEAAFLLGERVEGVDEALDAAPRLLSLGPRSLV
                     VTLGEDGAVFSGGESAQHLTAPKVKVVDTTGAGDAFVGALAVRLATDAPLEDAVGYAV
                     RAGAAAVTEAGAQGALPTPETVEAL"
     gene            complement(3668..4195)
                     /locus_tag="VNT12_07385"
     CDS             complement(3668..4195)
                     /locus_tag="VNT12_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45979.1"
                     /translation="MATSGVEENAMRLLGAFYDLSGGKLTEPVPIGGPEMPGQGAAPS
                     ADLDPDSPECDIAARYLVDQKFIAPADAASKYVITVAGFDRVREMRGIGGPTAPAGRS
                     RMSDKTQQRILTLLSIGISIGLSQPLARFIGEKIPERRGIKDDLTEAVLQGLVRAVAL
                     VVASVIVRALAASRR"
     gene            4399..6012
                     /locus_tag="VNT12_07390"
     CDS             4399..6012
                     /locus_tag="VNT12_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWI45980.1"
                     /translation="MSEARKTVLDNRYDVVRPLGSGGMGEVYLARDRVLGRDVALKVL
                     RKQYAGDDEFAERFKREAMSAASLSHPNIVQVYDRGQTEEGDSYIAMEYVPGGTLKER
                     ISREGPLETADAAGLGAQVAEALGAAHDRGMVHRDIKPQNVLLAARGGAKVADFGIAR
                     AGSSATISRTGSVMGTAGYMSPEQALGKPATPKSDLYSLGVVLYEALTGELPYTADNP
                     IAVSMKHVNEPLRPPIELNPRIPRGMNALVTKLLAKDPEDRYADADELADDLWRVNRG
                     LELVSAGVGRKQGPSTQRTAVASPNVTSEIPKPRPGPVPYRRRRGLPWLLIALAAILF
                     TLGVLGSVQAVLGPNAMKGWFDVFQGDKRSPGQPAGPEKVKVPEVDGLTRSVAEKRLT
                     EVGLEVGKVTSFPSDSVATGRVIEPGIVAGTPVEPGTDVNLTISSGPQPSSSASSSAS
                     AQSSPESGATASASASAQPAPREERQAVQTPEAGANDPRANVPRSKVQKNPPAQSQNA
                     AAPASEPVDDSGGQGRGRGRGRGGGPGED"
BASE COUNT         1141 a         2031 c         1962 g         1081 t
ORIGIN      
        1 cggcaatcag gggtacgctg cttggctccc tcatcctttg cgtgttcgct atcatcggcg
       61 acgtgctgct cgaagcactc gggatcgggc ttcccgcatt ccggatcgcc ggtggaatgc
      121 tgctctttct gctctcactc gacatggtct tcgccaggcc ttcgggaatg cgttccgcga
      181 ccgtgcagga acaagaggag gaagaatacc agcaggacat ctccgtcttc cccctggcca
      241 tcccgcttct cgctggtcct ggtggtatta ccaccgtcct cctcttcacc ggcggcagca
      301 gcgcgggcca ggtagcgttg ttcatggtgg tgttactcgt ggtcctgctt ctgaccctgg
      361 gctccctgct gctcgcccca cgcgtcatga ggctcctggg ggagacgggt gccaacgtgc
      421 tctcgcgcgt gctcggcgtt ctactcgccg cgctcgccgt gcagttcgtg atcgacggcc
      481 tccaatcaag cctcgcctga agggcggata tactcacggg catgtcgggc gcacccatag
      541 tgatcgtcgg tggggctacc tggtggccgg gtagttaccg caacctcgcg agcatgctgc
      601 aggagatctc cggctccgaa gcgtacattg tccccgtcac gccccttgac tggaccctag
      661 gatacttccg gggcttcggg caactggtct tcgaagtcgc gagtgcggtg gacagggcgc
      721 tgctcgagag cgaggcgaaa aaggcggtgc tcgtgggaca ctcggcgggc ggcctcgcgt
      781 gcagggtgta tatcggcgga gatcccccgt acggaggcag gcgttactcg ggacacagga
      841 gggtggagtc cctgatcaca ctaggcaccc ctcatactgt cgcaaataga caacgcctcg
      901 ccccgatcac ccgcgtgaac gagctcttcc cgggcgcctt ccacgctgga gctggtttgc
      961 gctacgtctc ggtcgctggt agcgtcgccg acggcggctc ctcacccagg gtccgcaagc
     1021 gttacgagcg gttcgtcgaa gacgggcggg tggctggaga cggggtcgtg cctgtcgatg
     1081 cggccgtgct tcccggctcg gagagcctga tcgtagacgg catccatcac aacaggcgtc
     1141 tcggccgttg gtacggctcg gacacggaga cagtgggacg atggtggccg caggagttgc
     1201 gcgcgggagg ctctctcgtg gcagacacgc gggcacggct tgctagaagg ggtatggatg
     1261 ccacctgacg gtacggacag gcgagagtat ccctggaaga tcagggaggc cgaggccgac
     1321 gccctgcgga gcgcgatgag ccactatcct accggcgtga cggtcgtgac gagcggaagg
     1381 gaggagaaag tcgaaggcat gaccgcgaat gctgtgatct ccgtctccct cgacccgctg
     1441 ctctttctgg tgagcgtcca caaagacgcg cgcatcaacc agcgcatcag ggaggaaggc
     1501 tcttttacgg tgaacctcct ggcggacgac caggagggac taagcaggct cttcgcctcg
     1561 ccagaacgct ccagcggact accggcgctc cactctctcg gcggcggcta tagctcggtg
     1621 ggggcgccgc ttgcggccgg cgcactcgcg gcgatcgact gcgagctggc cgagatctac
     1681 tatggaggcg accacgacct gttcctcggc agggtcgtcg aggtccgtct gggtgatacc
     1741 cgcaagggac cactgatttt ccacgagggc gactacccgg ccctgaaagc cgcccctcgt
     1801 cctgaggagt caggcgcctt cgtcgactcc gtcaggggct tcgacgcccg cctgaaccgc
     1861 ctgcgcgaca gaagacggcg ctagatgata cgcaaaaacc tgctctctat cgtctgtccc
     1921 cgtccctcgg ataccggggg tcttgtctac gccggtggta tcgagagaga cggtatgatc
     1981 ctggccggcg aaacgcgctc tgcggcaacg ggcgaaacct acaagataag agacggatat
     2041 ctggatctcc tgaaagcccg caccggcgca gatagtctcg ccaacctcac caacttcctg
     2101 ccgggcgcag gacgcggcta cgaacccctc tggcgcttac attccttgag cttccttacc
     2161 ggcgaaccct tcccgaacga gcgggagctg gagatcatca aacgcctcgt ccgccctgtc
     2221 agaggcggcc gctacctcga cctgggttgc tccgcaggcc tctacacccg cagcctcagc
     2281 ggcaacctcg acagcggtgt cgccgtcgga atagacatct ccccgtccat gctgagagaa
     2341 gcggcccggc gagcacgcgg gattggggcg aatccttcct tcgtcagggc gaacgcgaaa
     2401 caccttcctt tcctcgacac ctctttcgcg ggcgcggtct gcggcggctc cctcaacgag
     2461 ttcggcgacc cggcccgtgt cctgcgggag acatatagga tcctcgaacc tggcggacgg
     2521 ctcgccatca tgggcatact gcgtgcaggc acgccgcgtg gacgccggct ccagcgcata
     2581 ctctccacgg gcggcatcag gttcttcgac cccgacgagc tacagagcct gctcgaccac
     2641 gcgggcttcg agccagaccc gccccagaca tacggtcccg tctttttctc ggggtctacc
     2701 cgacggggtt gacgctaccg ctgcgctcta caaagcctcc actgtctctg gagtcggtag
     2761 cgctccctgg gcccccgcct ccgtcacggc ggccgccccg gcccgcacgg cgtacccaac
     2821 cgcgtcttcc agaggggcgt cggtggcaag ccttacggcc agggccccga cgaacgcgtc
     2881 tcccgccccc gtagtatcca cgactttcac tttcggggcc gtaaggtgtt gcgcggattc
     2941 gccgcccgag aacacggcgc cgtcttctcc gagcgtgacc acgagggatc ttggccccag
     3001 ggacaggagc ctcggggcgg catcgagtgc ctcatcaaca ccttcgaccc tctctccgag
     3061 gaggaaggct gcctcgtgct cgttgatcac caggggatca agcttctcga gcagtgtccg
     3121 cggaacctcg aacgggggcg ccaggttgac cagcgcgcgg gtcccgctcc ctgccgcgac
     3181 ctctacggcc cgcaggacga cttcggacgg gacctccatc tggactacga gtactcgcgc
     3241 ctctccgatg gacccggagg cgtcgtctac gtcctccgct gtgaggcagc ggttcgcgcc
     3301 tggtgcgacc gtgatcgcat tctccccatc cggagtgacg gttatgaagg ccgcgcctgt
     3361 ggacgaaccg ggaacctcct gcacaagggt cgtatcgacg cccttatcgg cgagcgaccg
     3421 taccagcggc ccgccgaacc cgtcatcgcc gacgcggccg aggaatgcaa cgctcgcgcc
     3481 gaggagggcc gcggcggcgg cctggttggc ccccttgccg ccgttgtggg tcgcgagacg
     3541 ggcgtccgtg accgtctcac cgggcgcggg acggcgctcc acgatcagga cgaaatcctg
     3601 gtttatagaa ccaacgacga agacgtcagc cactttgatt tcgccagaag ctttgggtgc
     3661 tggtggccta cctgcgactc gcggccagtg cccgaacgat cacggaggcc accaccagcg
     3721 ccaccgccct gactagtcct tgcaagacag cctcggtcag gtcgtccttt atgcccctgc
     3781 gttcggggat cttctcccct ataaacctgg ccagtggctg gctgaggccg atggagatcc
     3841 ctatggaaag cagcgtcaga atccgctgct gggtcttgtc gctcatcctg ctccttcctg
     3901 ccggcgccgt aggaccgcct atcccgcgca tctctctcac cctgtcaaag ccggcgacgg
     3961 tgatcacgta cttggacgcg gcatcggccg gtgcgatgaa tttctgatcg accaggtacc
     4021 tggccgcgat atcgcactct ggggagtcag ggtcgagatc ggcgctcggc gccgccccct
     4081 gtcctggcat ctctgggcct ccgattggga ccggctccgt taacttccct cctgagaggt
     4141 cgtagaaagc gccaagaagc cgcatcgcgt tctcttctac cccgcttgta gccaagatcc
     4201 cacccttaca tgcctccacc atctcgtcgt ctgcctcatc gccccaccga gtccgttatc
     4261 gcattcgacc gggcaccttc acagtacccg accctggcgc tgcctaaccc tcatcctcct
     4321 taaggcagcc ttcgtaaggt tccgaccgga agtgtagaat gacgcgggta acgttttaga
     4381 agccggacca agggcaggat gtcagaggca agaaaaacag tcttggacaa ccgctacgac
     4441 gtggttcgac cactcggtag cggaggcatg ggcgaggtct acctggcccg cgacagggtg
     4501 ctcgggcgcg acgtggcgct gaaggtgctt aggaaacagt acgccggcga cgatgagttc
     4561 gccgagcgct tcaagcgcga ggccatgagc gcggcgtcac tctcccaccc gaacatcgtc
     4621 caggtctacg acaggggcca gacggaagaa ggggactctt acatagccat ggaatacgtg
     4681 cctggcggca cgctcaaaga gaggatctcc agggagggcc cgctcgagac ggcggacgcc
     4741 gccgggcttg gtgcgcaggt cgccgaggcg cttggagcgg cccacgacag gggaatggtc
     4801 caccgcgaca taaagcccca gaacgtacta ctcgcagcca ggggcggagc gaaggtggca
     4861 gacttcggca tcgcaagggc cggctcctcc gcgacgatct ccaggacggg gtccgtgatg
     4921 ggtaccgccg gctacatgtc ccccgaacag gcccttggca agcctgcgac ccctaaaagc
     4981 gacctctact ccctcggagt ggtgctctac gaagcgctca caggcgaact gccgtacacc
     5041 gccgacaacc cgatcgccgt ctccatgaag cacgtgaacg agccgctacg gccgcccata
     5101 gaactaaacc cccggatacc cagaggcatg aacgccctcg tgacgaagct cctggcaaaa
     5161 gacccggaag accgctacgc agatgccgac gagcttgcgg acgacctctg gagggtgaac
     5221 cgtggactcg agctggtctc ggcgggtgtc ggtaggaagc agggaccgag cacgcaacgt
     5281 actgcggtcg cgtcgccgaa cgtcacgagc gagataccca aaccccggcc aggcccggta
     5341 ccatatcgac gcaggcgagg cttgccctgg ttgctgatcg cgctggctgc aatcctgttt
     5401 accctcggcg tgctcggcag cgtccaggct gttctgggtc cgaacgccat gaaaggctgg
     5461 ttcgacgtct tccagggaga caagcgatct cctggccagc ccgctggacc ggagaaggtg
     5521 aaggtacccg aagtggacgg gctcaccagg agcgtggcag agaagaggct caccgaagtg
     5581 gggctcgagg tcgggaaggt cacctcgttt ccgagcgata gtgtcgccac aggcagggta
     5641 atcgagccag gcatcgttgc aggcacccca gtcgagcctg gcacagacgt gaacctgact
     5701 ataagctcag gaccacaacc ttcctcatcc gcgagctcgt cagcgtctgc ccagtctagt
     5761 cccgaatccg gcgccaccgc ctcggcatcc gcttctgcgc agcctgctcc gcgcgaggag
     5821 cgccaggcgg tccagacgcc ggaggccggg gcgaatgacc ccagggccaa cgtacccaga
     5881 tccaaggtgc aaaagaatcc acctgcacaa agccagaacg cggctgcacc ggcaagtgag
     5941 cctgtggatg acagcggggg acaaggtcgc ggtcgaggac gtgggcgcgg tggaggaccc
     6001 ggggaggatt gatgcgattg gcagaggtat cgacctgctg cgattcacgc atccgcccac
     6061 gagtgttatc gctaaaagcg ataacaagaa gctgacaagt tgaggcctga aaggccgaag
     6121 cgtgcttact ggctgaattg ctgacacaat agtgggtgtg taggccgagt tcatttcggg
     6181 agggagagaa gcatgaaggc gatccggata cacga
//
LOCUS       DATMJA010000308         4370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_692030, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000308 DATMJA010000000
VERSION     DATMJA010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692030"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VNT12_07395"
     CDS             <1..820
                     /locus_tag="VNT12_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI45981.1"
                     /translation="AVSCERVSFSYPGSGRPALSEVSLDLRPGEYVGVVGPNGGGKST
                     LVRLLNGLLRPESGRVLVSRRDPVTEPFEVRKHLGVLFQNPENGLVAPFVEDDVAFGL
                     ENLGVPREEMRERVARAIRTVGLEGYERREPHTLSGGEKQRVALAGLLAVEPQILVLD
                     EPTSMLDSAGRREVLKYLQDLRSEKTVLHVTHHLEELTGADRILVLNGGELVADETPE
                     RFFSNADLLRENRLVLPVVPRLALELGLAPAKLRTPEELAEAILARTQSGTRSR"
     gene            817..1632
                     /locus_tag="VNT12_07400"
     CDS             817..1632
                     /locus_tag="VNT12_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI45982.1"
                     /translation="MKLELRDVRHVYARGTPFEVEALRGVSLVVEPGEVLAIVGGTGS
                     GKSTLVQHMNLLLVPTSGEVLLDGVDATTLKKSELRRRVGLVFQFPESALFAPTVEED
                     VAFAPRQLALSEEEVRERVLESLGALGAEELAAHSPFALSSGEKRRAAIAGVLAMRPE
                     VLVLDEPTAGLDPATREDLLTLILGLRESGVSVVLVSHDLDEVAEVADRVCALHEGRV
                     SAVGTAAEVFYGDPTQAPATVQVVSLLRGSRPEIGDPVRFGETLDALRALQGT"
     gene            1634..2422
                     /locus_tag="VNT12_07405"
     CDS             1634..2422
                     /locus_tag="VNT12_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HWI45983.1"
                     /translation="MTSRSLGQFYPVSSPVHALDPRAKLLATAVFVVGLFLIDSTVGL
                     LFVAVALVALVAASRIPAGTFLRLLRPVLFVVALTVLFQVFFSREGVMLFEWGFLEVH
                     SGGLRLGFFLALRILLLVSSAGLLTATTAPVALADGIEDLLSPLKRVRFPAHEVAMMM
                     TIALRFIPTLGEEAEKIKGAQAARGADFSEGGPLRRARSFLPVLIPLTVGAFRRADEL
                     ADAMESRGYRGGEGRTRYRELRFRVSDALALALTLFLLLVGILL"
     gene            2419..3180
                     /gene="truA"
                     /locus_tag="VNT12_07410"
     CDS             2419..3180
                     /gene="truA"
                     /locus_tag="VNT12_07410"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWI45984.1"
                     /translation="MKLAGLVEYEGTDFAGWAAQPERRTVEGVLCEALSTVLRQPLKM
                     SVAGRTDAGVHASGQVVSFEVDTELRPALISYKTTAVLPKDVALRRCIAVPDAFDART
                     DARSRTYEYRLVNDEIRSPLERHHAGYAPQRLDLDLLSAAARLVEGTHDFKAFTPQKS
                     YHVRFERIVTSSRWTRRDDLLRYRITADSFLYGMVRTLVGTMLEVAGGKRYLREFDRL
                     LSGGKRSESGPAVSSRGLTLVGVGYDDPNLWRSER"
     gene            3177..3845
                     /locus_tag="VNT12_07415"
     CDS             3177..3845
                     /locus_tag="VNT12_07415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HWI45985.1"
                     /translation="MNTHDTRELLREIDTYVEDLFSPSDKILEAALRDSRRAGLPEIN
                     VSPNEGRLLQLLTEIAGARRILEIGTLGGYSAIQFARALPDDGALISLEIDEHHAGVA
                     RSNIERAGLSEKVEIRVGDAHELLASLIEDDEGPFDVIFIDADKESYPEYLDASLRLV
                     RPGSLILGDNTIRGGTVLDPKDTTARATREFNERIARDPRLLSIALPLIRERIDGLTI
                     ARVM"
     gene            3971..>4370
                     /gene="rplM"
                     /locus_tag="VNT12_07420"
     CDS             3971..>4370
                     /gene="rplM"
                     /locus_tag="VNT12_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWI45986.1"
                     /translation="MMKSYMARPLEVERKWYVVDAEGKHLGRLATEIVRVLRGKNKPQ
                     YTPHVDVGDFVVVVNADRVAVTGRKAEQRVYRRHSGYPGGMKETSYEQMITRKPTEVL
                     RKAVYGMMPKTRLARKQFKKLKIYAGPEHPH"
BASE COUNT          806 a         1404 c         1401 g          759 t
ORIGIN      
        1 cgccgtctct tgcgagagag tatccttctc ctaccccggc tccggacggc ctgccctctc
       61 ggaggtgagc ctcgaccttc gcccgggaga atacgtcggc gtggtaggcc cgaacggcgg
      121 cggcaagtcc accctggtcc gcctcttgaa cggcctcctg aggcccgagt cgggccgcgt
      181 gctcgtgtca cgccgcgacc ctgtcacgga acctttcgag gtccgcaagc acctcggcgt
      241 ccttttccag aatcccgaga acggcctcgt cgcccctttc gtcgaggacg acgtcgcttt
      301 cggcctggag aaccttggcg tcccccgaga ggagatgcgc gagagggtcg ccagggccat
      361 acggaccgtc ggcctcgaag gctacgagcg ccgcgagccc catactctct cgggcggcga
      421 gaagcagcgc gtcgcccttg ccggcctcct ggccgtcgaa ccccagatac tcgtcctcga
      481 cgaacccacc tccatgctcg actccgcggg acgcagggag gttcttaagt acctccagga
      541 cctgcggtca gagaagaccg tccttcacgt aacccaccat ctcgaagagc tcacaggcgc
      601 cgaccgtatc ctcgtcctga acgggggaga gctcgtggct gacgaaacac ccgaacgatt
      661 cttctccaac gcggaccttc tgcgggagaa caggctggtt ctgcccgtcg tgccgaggct
      721 cgcactggag ctcggcctcg cgccggcgaa gctacgcacg cccgaagagc tggccgaggc
      781 catactcgcc agaacacaga gcgggacgag gtctcgatga agctggagct cagggacgtc
      841 aggcatgtct acgcaagggg cacccccttc gaggtcgagg cgttgcgagg cgtgtcgctc
      901 gtcgtggagc cgggagaggt gctcgcgatc gtcggcggta cgggctcggg caagagcacg
      961 ctcgtacagc acatgaatct gctcctcgtt ccgacgagtg gcgaggtgct cctcgacggc
     1021 gtggatgcga cgacgctcaa gaagagcgag ttgcgacgca gggtcggcct cgtcttccag
     1081 ttccccgaat cggcgctctt cgccccgacc gtcgaggagg acgtggcctt tgccccacgt
     1141 cagctcgctc ttagcgaaga ggaggtcagg gagcgggtgc tggagtcgct gggggcgctt
     1201 ggggcggagg agctcgcggc gcattcacct ttcgccctct cgagcggcga gaagcggcgg
     1261 gccgccatcg ccggggtgct cgcgatgagg ccagaggtcc tcgtcctcga cgagccgaca
     1321 gccggcctcg acccggccac cagggaggat cttctgaccc tgatcctggg cctgcgcgaa
     1381 tcaggagtct ccgtcgtcct cgtctctcac gacctggacg aggtcgctga agtcgccgac
     1441 agggtgtgcg cgctacacga aggcagggta agcgccgtcg ggactgcggc cgaggtgttc
     1501 tacggagatc ctacacaggc cccggcgacc gtacaggtgg tctccctgct cagagggtcg
     1561 agaccagaga tcggtgaccc cgtgcgcttc ggggagacgc tcgacgcgtt gagggctctg
     1621 caaggtacct gaggtgacga gcaggagcct cggccagttc tatcccgttt cctcgcccgt
     1681 gcacgcgctc gacccgaggg ccaagctcct ggccacagcc gtgttcgtcg tcgggctgtt
     1741 cctcatagac tcgacggtgg ggctcctctt cgtcgccgtg gcgctcgtgg cgctcgtggc
     1801 ggcgagccgt attccggcag gcaccttttt gcgactcttg cggcccgtgt tgtttgtcgt
     1861 cgcgctgacg gtcttgttcc aggtcttctt ctcgcgggag ggtgtcatgc tcttcgagtg
     1921 gggcttcctg gaggttcatt ccggcggatt gaggttgggt ttcttcctcg ctttgcggat
     1981 actcttgctc gtctcatcgg cagggttgct tacggcgacg acagccccgg tggccctcgc
     2041 tgatgggatc gaggaccttc tttcgcccct gaagagggtg cggttcccgg cgcacgaggt
     2101 cgcgatgatg atgaccatag cgctccgttt catccccacg ctcggggagg aggccgagaa
     2161 gatcaaaggt gcccaggccg cccgcggcgc ggacttctcc gagggagggc cgcttcgcag
     2221 ggcacgctcc ttcctccccg tactcatacc gctgaccgtc ggcgccttcc ggcgcgccga
     2281 cgagctcgcc gacgcgatgg agagcagggg ctataggggc ggcgaaggcc gcacccgcta
     2341 ccgtgagctg cgcttccggg taagcgacgc gctcgcactg gcactcacgt tgttcctcct
     2401 gctggttggg atcctgcttt gaagctcgcc gggctcgtcg agtacgaagg caccgacttt
     2461 gcaggatggg ccgcccaacc agaacgaaga accgtcgagg gtgtactctg tgaggcgctc
     2521 tctaccgttc tgcgccagcc gctcaagatg agcgtcgcag gccgtactga tgcaggggtg
     2581 cacgcgagcg ggcaggtagt ctccttcgag gtggacacgg aactcaggcc ggccttgatc
     2641 tcctacaaaa ccacggcggt actgcccaaa gacgtggcac tccgtcgttg catcgctgtc
     2701 ccggacgcgt tcgatgcccg cacggacgcg aggagccgga cgtacgagta taggttggtc
     2761 aacgatgaga tcaggtcacc cctggagcgc caccacgcgg gctacgcacc gcagagactc
     2821 gacctggatc tcctctctgc agcggcaagg ctggtggaag ggacccacga cttcaaggcc
     2881 ttcacccccc agaagagcta ccacgtccgc ttcgaacgaa tcgttaccag ctcgcgctgg
     2941 accaggcgcg acgacctgct aaggtaccgc ataaccgccg actcgttcct gtacggtatg
     3001 gtccgcaccc tggtcggcac catgctggag gttgcaggcg gaaagaggta cctgagggag
     3061 ttcgaccggc tgctctcggg aggaaagcgc agtgaatcag ggcccgccgt tagcagccgc
     3121 gggctcacgc tggtcggcgt gggctacgac gacccgaacc tatggaggag tgagcgatga
     3181 acacacacga tacgagagag cttttgcggg agatcgacac ctacgtcgag gaccttttct
     3241 ccccatcgga caagatcctc gaagcagccc tgcgagactc caggcgggcc ggactgcctg
     3301 agataaacgt ctcgcccaac gaaggaaggc tgctgcaact gctcaccgag atagccgggg
     3361 cgcggcgcat actggagata ggcaccctgg gcggctacag cgccatccag ttcgcccgtg
     3421 cccttcccga cgacggagcc ctgatctccc tggagataga cgagcatcac gccggagtcg
     3481 cccgcagcaa catcgaacgt gccggactct ccgaaaaggt cgagatccgg gtaggcgacg
     3541 cccacgaact cctcgcttcg ctaatcgaag acgacgaagg ccccttcgac gtgatcttca
     3601 tagacgccga caaagagagc taccccgagt acctagacgc gtccctgcgc ctagtacgac
     3661 caggctctct catcctcggc gacaacacca taagaggcgg caccgtactc gaccccaagg
     3721 acacaacggc ccgggcgacg cgcgagttca acgaacgtat agccagagac ccaagactcc
     3781 tcagcatcgc gctacccctg atccgagagc gcatagacgg cctcacgata gcacgggtca
     3841 tgtaacatca agtcacggca tacggaaggc ccgaggcctt ccgtatgacg ccaaacgctg
     3901 gtccttttgc cgatgtatgc tactatttga tccgttcgaa ggcctttgaa gttcgcacaa
     3961 gctccggagg atgatgaaga gttacatggc gcggcccctg gaggtcgagc gtaagtggta
     4021 tgtcgtcgac gccgagggaa agcacctcgg gcgcctggcg accgagatcg tacgcgttct
     4081 gcgcggcaag aacaagccgc agtacacgcc gcacgtcgat gtaggcgact tcgttgtcgt
     4141 tgtgaacgcg gacagggttg ccgtcacggg caggaaggcc gagcagaggg tctacaggcg
     4201 gcactcgggt tatcccgggg gcatgaaaga gaccagctat gagcagatga tcacgcgcaa
     4261 accgaccgag gtcttgcgca aggccgtcta cgggatgatg ccgaagacgc gcctcgctag
     4321 aaagcagttc aagaagttga agatctacgc gggacccgag cacccacact
//
LOCUS       DATMJA010000309         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_219242, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000309 DATMJA010000000
VERSION     DATMJA010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219242"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..85
                     /locus_tag="VNT12_07425"
     tRNA            12..85
                     /locus_tag="VNT12_07425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Ile,seq:cat)
     gene            407..847
                     /gene="mraZ"
                     /locus_tag="VNT12_07430"
     CDS             407..847
                     /gene="mraZ"
                     /locus_tag="VNT12_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWI45987.1"
                     /translation="MALLGEYEHTLDEKGRMTLPSRLRSYFEGGIVVTKGVDRCLFAF
                     PPEEWAAFKANIKANADLSARGRQLSRMFFSMAFEATLDRQGRVLIPTKLARYAGLQR
                     DVTITGVDDRLEIWDTEEWDRYVSGADDSFSEIVEEFAGRDLGG"
     gene            847..1782
                     /gene="rsmH"
                     /locus_tag="VNT12_07435"
     CDS             847..1782
                     /gene="rsmH"
                     /locus_tag="VNT12_07435"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWI45988.1"
                     /translation="MATGHHPVMLERAVAALGLAPEDSVVDATFGGGGHSRLIIAGLG
                     PGGSLIGIDRDPEAAGRASDLLQDPRFEFVSGAYDLALWAMVEERRRVDAILFDLGLS
                     SFQVDEPGRGFSYVHEGPLDMRMDPRSGTTAADFLNTVEVGELSRMLAEYGDVQRGEA
                     GRVAREVVRRRPLAVTTDLYEAVRAAVGWKERGGNPAKRIFQAVRIRVNDELGSLSRA
                     LDASERLLTPGGRLVVITFHSGEDRLVKRFISGREGRCVCPPELPVCVCGAKPVFRRG
                     VVLTPSVREVEENPRSASARLRAAIRTAEPPVDAS"
     gene            1782..2219
                     /locus_tag="VNT12_07440"
     CDS             1782..2219
                     /locus_tag="VNT12_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45989.1"
                     /translation="MAASQRRYKATLPTEPAWPAQKRAQDRSQDRILRRKAASRRRRF
                     AMLVVVPVLLMLGSVYLNTLAAGLDARAAELDVRLDRAREVGERLEVKVAELSGAERI
                     RALAAEKLGMHEPKSTTLKVYGVRAEDGKSDGAPQEGGRPRQR"
     gene            2419..>2601
                     /locus_tag="VNT12_07445"
     CDS             2419..>2601
                     /locus_tag="VNT12_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI45990.1"
                     /translation="MVAAVFAVTVLLLGGRAVHISLTEDASYRAFAEERAGGTTPVAA
                     PTRGSIVSADGRELATS"
BASE COUNT          497 a          748 c          905 g          451 t
ORIGIN      
        1 ggtaccccct agggcccata gctcagttgg ttagagcatc cgactcataa tcggacggtc
       61 ccaggttcga gtcctggtgg gcccacaaac gcagtccaaa atatcacccc cgcactagac
      121 cccaacttat ttccacacac ccaatatgtt gggggccttc tcatatgcga ccgacatgtt
      181 acacctgggt tacagttcca ccatgtagag gaaccttccg taagctagca aatcgcgtgc
      241 tctaagtagg ggtcctatgc cctggggtag agggatcaag gcacctcaac ggacgaaata
      301 cggtctttca gaggcgttct cggcctctca gattgcgaat tttccgtgca tatgcaagaa
      361 tttcgctgtt gtgggtcaaa gtggagcgta gtgggaggac acggctctgg cccttttagg
      421 cgagtacgag cacacgctgg atgagaaagg gcgcatgacc ctcccttcgc gactccgctc
      481 ctatttcgag ggtgggatcg ttgtaaccaa gggggtggac aggtgtctgt tcgccttccc
      541 gcctgaggag tgggcggcgt tcaaggccaa cataaaagcc aatgcggatc tctcggccag
      601 ggggcgccag ctctcgcgga tgttcttctc catggctttc gaggccacgc tagacaggca
      661 gggtcgggtc ttgatcccaa ccaagctcgc caggtacgcg ggactgcagc gtgatgtgac
      721 catcacgggt gtggatgacc gcctggagat ctgggacacg gaggaatggg accgctacgt
      781 ctcgggtgct gacgactcct tctccgagat cgtcgaggag ttcgcaggaa gggatctcgg
      841 cgggtgatgg caacaggaca ccatcccgta atgctcgaga gagcggtggc cgccctgggg
      901 ctggctcccg aagactctgt ggtagacgcc accttcggcg gtggcggcca ctccagactc
      961 ataatcgccg gtctcgggcc gggtggtagc ctcattggca tcgaccgcga ccccgaggcg
     1021 gcagggcgag cctctgatct gctgcaggac ccgcgcttcg agttcgtctc gggcgcctac
     1081 gacctggcgt tgtgggcgat ggtcgaggaa cgccgacgtg tcgatgcgat cctgttcgac
     1141 ctcggcctct ccagtttcca ggtcgacgag ccaggacgcg ggttctcata tgtgcacgag
     1201 ggaccactcg acatgcgtat ggacccacgc tcgggtacaa cggccgcgga cttcctgaac
     1261 accgtcgagg tcggggagct gtcgaggatg ctcgcggagt acggcgacgt gcagcgcggt
     1321 gaggcagggc gtgtggcgcg ggaggtcgtc cggaggcgac cgctcgcggt tacgacggac
     1381 ctttacgagg ccgtgcgggc ggccgtgggc tggaaggaga gggggggcaa cccggcgaag
     1441 aggatcttcc aggccgtgcg gatcagggta aacgacgagt tgggcagcct cagccgggcc
     1501 ctggatgcct cagaacggct actcacacca ggtgggaggc ttgtggtcat cacgttccac
     1561 tcgggggagg atcgcctcgt aaagcgattc atctcagggc gggaggggcg gtgtgtgtgt
     1621 ccgccggagc tgccggtgtg cgtttgtggt gcaaagcccg tcttccggcg aggagtggtt
     1681 ctgaccccat cggtacgtga ggtagaggag aacccgcgca gcgcatcggc caggctgcgg
     1741 gcggcgataa ggacggccga gcctccggtg gatgctagct gatggctgcc tcccagcgca
     1801 ggtacaaggc tacgctccct accgaaccgg cgtggcccgc acagaagcgc gcccaggacc
     1861 gctcgcagga tcgcatccta aggcggaagg ctgccagccg gcgtcgccgg tttgcgatgc
     1921 tggtcgtggt gcccgtgctg ctgatgctgg ggagcgtcta tctgaacacg ctcgccgcgg
     1981 gactcgacgc cagggccgcc gaactcgacg tcaggcttga tagggccaga gaagtagggg
     2041 agagactcga ggtcaaagtc gccgaactct cgggcgcgga gaggatccgg gccctcgcgg
     2101 cggagaagct tggtatgcac gagccgaaga gcactacctt gaaagtctat ggcgtaagag
     2161 cggaggatgg gaaaagcgat ggggcaccac aggaaggggg aagaccgcgg cagcgttgat
     2221 cggccagggc ctcgcaacgc aaggattcgc cgtggcaaga agaccccccc tcgcaccaaa
     2281 aacaaagggc ctaagtcctc tggtggcaaa gtcataccgc tccgcggcca gaggcgggcc
     2341 agcggcgaga ccagggcgcg tcgcgcgtcg tcgatggccc ccgaagctag gactactgtg
     2401 ggccacagga ggctgtggat ggtagcggcg gtgttcgcgg tgacggtact tcttctcggt
     2461 gggagggccg tgcacataag cctcacggag gacgcgagtt acagagcctt cgccgaggag
     2521 cgggccggtg gaacgacccc ggtcgccgcg ccgacccgcg gcagcatagt gagtgccgac
     2581 ggaagggagc tcgcgaccag c
//
LOCUS       DATMJA010000310         2417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_396706, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000310 DATMJA010000000
VERSION     DATMJA010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396706"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VNT12_07450"
     CDS             <1..1285
                     /locus_tag="VNT12_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWI45991.1"
                     /translation="PVWYGPDGEVMASKESPGAGFEQDPDVFDTWFSSSLWPIATLGW
                     PDKDAEDLRYFYPTDLLSTAREIMYLWVARMIMMGLHFEGEIPFEKVNVHSIVLAEDG
                     TKMSRSKGNTIDPLELFDEYGTDAVRFGLLYQSSTQDFPYSRERAEMGRSFVTKLWNA
                     MRFVLAYPEAEENGELSPSDRWILSEFSRTVREYDAYLEECEFSEAMRRLYAFAWNQF
                     ADWYIEIAKAAPSSATPRVLREVFHGILKLLHPVMPFATEEMASVMGEQALLATQRYP
                     ESDPALEDEEADRLLDRTRRAVSAVRSFRAESKVDGELEGLMPKGIDEGVFAALAGVR
                     PAEGGNGASRASLPAGDVVVEILLSDEMRRGEIERLGREISRAEQEVARAQSKLANEK
                     FVTNAPERVVAGERKKLEVNSRMLETLSRRLEEYI"
     gene            1304..>2417
                     /locus_tag="VNT12_07455"
     CDS             1304..>2417
                     /locus_tag="VNT12_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HWI45992.1"
                     /translation="MTSFAQVCAELDRRRHITLGFERIEAMLTAMGHPERDLPGLVQV
                     VGTNGKGTTALALGVALEEMGHPSGAYLSPHVLSYTERVVLRGGQVSEEDFAAAMSET
                     IELADRKGIPASQFELLTAGALGMFAAAGLSWAVLEAGLGARHDATSVVRPDAVVLTN
                     VSIDHTEYLGETVEEIAEEKLASLSPGATLFLGTDEGRIIDLAHSVCRRHRASLVLPG
                     EAGENASYSGLAPYAAGDVRLGIAVAESLLGRSIPAEVEHKVALAVGEALPGRFEVHE
                     VEGVPVVVDGGHNAAGLSAALTGMRAVYGERPLGVVFGVLREKDAAIMLAGLKGEADV
                     IVLTRPESERAADPAHLEHSLLDRGERRALVVESPVR"
BASE COUNT          451 a          749 c          793 g          424 t
ORIGIN      
        1 accggtgtgg tacggccctg acggtgaggt aatggcttcc aaagagtcgc cgggcgctgg
       61 tttcgagcag gaccccgacg tgttcgacac ctggttctcc agcagcctgt ggcccatcgc
      121 gaccctgggg tggcccgaca aagacgcaga agacctgcgg tacttctacc cgaccgacct
      181 cctctcgact gcgcgtgaga ttatgtacct ctgggtggcc cgcatgatca tgatgggcct
      241 ccatttcgaa ggcgagatcc ccttcgagaa ggtgaacgta cactctatcg tgctcgctga
      301 ggacgggacg aagatgagcc ggtccaaggg gaacaccata gatcccctgg agctcttcga
      361 cgaatacgga accgacgccg tccgcttcgg cctcctctac cagtcctcga cccaagactt
      421 cccgtactcc cgcgagcggg ccgagatggg cagatccttc gtcacaaagc tctggaacgc
      481 catgcgcttc gtactcgcct acccggaggc tgaagaaaat ggagaactct cgccttctga
      541 ccgttggatc ctctctgagt tcagccgcac ggtgagggag tatgacgcct acctcgagga
      601 gtgtgagttc tcggaggcga tgcgtcggct ctacgctttc gcctggaacc agttcgccga
      661 ctggtatatc gagatagcga aggcggcccc ctcgtcagcg acgccccgcg tcctgcgcga
      721 ggtcttccac ggtatcctga agctcctcca ccccgtcatg cccttcgcga cggaggagat
      781 ggcaagcgta atgggagaac aggcactgct cgcgacgcaa cgttatcctg agtctgaccc
      841 cgcgctcgaa gacgaggagg ccgaccgtct gctcgaccgc acaaggaggg ccgtctcggc
      901 ggttcgctcg ttcagggccg agtcgaaggt agacggcgag ctcgaagggc tgatgccaaa
      961 gggcatcgac gagggcgtct tcgccgctct ggccggggtg agacccgcgg agggcgggaa
     1021 cggcgcgagc cgcgcttcgt tgcccgcggg ggacgtggtc gtggagatcc tgctctccga
     1081 cgagatgcgc cgcggcgaga tagagcgcct ggggcgcgag atctctcgcg ccgagcagga
     1141 agtggcacgc gcacagagca agctcgccaa cgagaaattc gtgaccaatg cgccggagag
     1201 ggttgtcgcc ggcgagcgca agaagctgga agtcaactcc aggatgctcg agacgctctc
     1261 tcgccgtctc gaagaataca tctaggagcc ggccattcct accatgacct ctttcgccca
     1321 ggtctgtgcc gagttggacc ggcgccgtca catcaccctc ggtttcgagc ggatagaagc
     1381 catgctcaca gccatgggtc atcccgagag ggacctccct ggcctggtac aggtcgttgg
     1441 cacgaacggt aagggcacta cggccctggc tctcggggtg gcactggagg agatggggca
     1501 tccgtcaggc gcttacctct caccgcacgt cctctcctac accgaacggg tcgtactccg
     1561 aggcggccag gtatcagaag aggatttcgc agccgctatg agcgagacca tagagcttgc
     1621 ggacagaaag ggcatccctg cctcgcagtt cgagctgctt accgctggcg ccctcgggat
     1681 gttcgcggca gcagggctgt cgtgggctgt cctcgaggca ggcctcggcg cgcgccacga
     1741 cgccacctct gtcgtcaggc ccgacgccgt ggtgctcacc aacgtttcca tcgatcacac
     1801 cgagtacctg ggtgagacgg tcgaggagat agccgaagag aagctcgcca gcctgagtcc
     1861 cggcgcgact ctgtttctcg gtacggacga agggcgaatc atcgacctcg cgcacagcgt
     1921 atgccgccgg cacagagcta gcttggttct gcccggcgaa gccggcgaga atgcctcata
     1981 ttcaggcctc gcaccgtacg ccgctggcga cgtgcgcctg gggatcgcgg tagccgagtc
     2041 actgctggga agaagcatcc ccgccgaggt cgagcataag gtcgcgctgg ctgtcggaga
     2101 ggcgcttccc ggacggttcg aggtacacga ggtagagggt gtccccgtgg tggtcgacgg
     2161 tgggcataac gcggccgggc tctcggctgc gctcaccggg atgcgcgccg tgtatggcga
     2221 gcgtccattg ggtgtcgtct tcggcgtctt gcgagagaag gacgccgcaa ttatgcttgc
     2281 cgggttgaaa ggtgaagccg acgttatcgt gctgacgcga cctgagagcg aaagggccgc
     2341 cgacccagcg cacctcgagc acagcctcct cgaccgcggt gagaggagag ccctggtagt
     2401 cgagagccct gtgagat
//
LOCUS       DATMJA010000311         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_337781, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000311 DATMJA010000000
VERSION     DATMJA010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337781"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VNT12_07460"
     CDS             complement(<1..709)
                     /locus_tag="VNT12_07460"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI45993.1"
                     /translation="MSKRADEKGVVGERRWLWPSFALPGVVWLIVLFVTPLYAILAMA
                     MGVSDPIFGDTLPVWNPLQWNYATFGDVLGELFTGQLGVVFLRTLLYVVVATALSLLI
                     GYPVAYYVARHAGRYRVLLLLLIIAPFWINYLMRMLAWVNLLSTDGYVNQVLMFVGIL
                     DAPREWLVGKPETVVLGLVYGYVPFLILPLFAALDRIDQSVIEAARDLGASPAQAFYR
                     VTLPLSYQGMLAGCVIIT"
     gene            complement(714..1979)
                     /locus_tag="VNT12_07465"
     CDS             complement(714..1979)
                     /locus_tag="VNT12_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI45994.1"
                     /translation="MAFHPRERSPWPRRMSRREALRLMAGTAAAGGMLAGCGSGAGSS
                     TSASRVVVGTREDPVKQPLFDDNPMIDSGLEPEKGPLRLYNWSDYIYTRVLKDFEEEF
                     GVEVELTTFYNLEEATQKLRTGKVDFDVFVPTAEIIPKFVAGKLLQPLNHDYLPNLKK
                     NVWPMLADPYYDRGSRYTVPYTVYLTGIGWRTDKVDADVAAMDNPWEAFWDPEYKGLV
                     GLYDDYRETIGVGMFKSGITEINSANPDDLKAAQNNLIELTDLVDIRYTIDGAYSKLP
                     EGVFGIHHAWSGDMIYAPYYMPKGDDPSVLRYVWPPKAQGGAGGYVSNDNLAVLNGAE
                     NPVLAHMFLNYMLDDKVAIKNFGWNAYQPPMKTLDPSKLVSDGTIRKNLATTVIVQSD
                     FDMGQIPQQLTPDEDKRWLDTWSKVQQGG"
     gene            complement(2026..>2688)
                     /locus_tag="VNT12_07470"
     CDS             complement(2026..>2688)
                     /locus_tag="VNT12_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI45995.1"
                     /translation="RPKLTQKRRTQMLEAAMEVIAERGLCETRIADVAERAGLSPALV
                     VYYFGSKETLLTEALAYAEDLFYMEAFRELTGIEDASRRLVRLIELACPPEVRRESDE
                     YWTLWVELWSRALRNDEAARKREALERRWRNTIAEVVREGQRSGEFGQCDPERFATYL
                     SALMDGLALQVLFGDPAVNGELVYEMCLDAAARELGFEPDASRRRDKEREAEQEELAA
                     EG"
BASE COUNT          488 a          895 c          808 g          497 t
ORIGIN      
        1 gcgtgatgat cacgcacccc gcgagcatcc cctggtaaga gaggggcagc gtgacgcggt
       61 agaaggcctg cgcggggctg gccccgaggt cgcgcgccgc ctctatgacg ctctgatcga
      121 tcctgtccag ggcggcgaag aggggcagga tcaggaacgg cacgtagccg tagacgaggc
      181 ctagcacgac ggtctcgggc ttgcccacga gccactcccg cggcgcgtcg aggatgccca
      241 cgaacatcaa aacctggttt acgtagccgt cggtggagag caggttcacc cacgcgagca
      301 tccgcatgag gtagttgatc cagaacggcg cgatgatgag cagcaagagc aatacccgat
      361 accgcccggc atgcctagcg acgtagtagg ccacggggta cccgatgagc aggctcaagg
      421 ccgtcgcgac gacgacgtag aggagcgtgc gcaggaagac caccccgagc tggccggtga
      481 acagttcgcc gagcacgtcc ccgaaggtcg cgtagttcca ctgcagcggg ttccaaacgg
      541 gcagcgtgtc cccgaagatg ggatctgaga cccccatcgc catcgcgagg atcgcgtaga
      601 ggggcgtaac gaagaggact attagccaga ccacgccagg aagggcgaag gagggccaca
      661 accagcgtct ctcgccgacc actcccttct cgtcggccct tttgctcacg gtgctagccg
      721 ccctgctgaa ccttcgacca tgtatccagc caccgcttgt cctcatctgg cgtgagctgc
      781 tgcggtatct ggcccatgtc gaagtcgctc tgcacgataa ccgtcgtcgc caggttcttg
      841 cgaatggtcc cgtccgacac cagtttgctc gggtccaggg tcttcatagg cggctggtag
      901 gcgttccaac cgaagttctt gatggcaact ttgtcgtcta gcatgtagtt gaggaacatg
      961 tgggccagga cagggttctc ggcgccgttg agcaccgcga ggttgtcgtt ggatacgtag
     1021 cctcccgccc cgccttgcgc cttcggaggc cacacgtagc gcagcaccga agggtcgtca
     1081 ccctttggca tgtagtaggg cgcgtagatc atgtcgcccg accatgcatg gtggatgccg
     1141 aagacgcctt cgggcagctt ggagtaggca ccatcgatgg tgtagcggat gtctaccagg
     1201 tccgtgagct cgataaggtt gttctgggcg gccttcaggt cgtccgggtt tgcgctgttg
     1261 atctcggtga tgccgctctt gaacatcccg accccgatcg tctccctgta gtcgtcgtag
     1321 agtcctacca atcccttgta ctcgggatcc cagaacgcct cccaggggtt gtccatggcc
     1381 gcgacatcgg cgtcgacctt gtccgttcgc caaccgatcc cggtcaggta gacggtgtag
     1441 ggcaccgtgt agcgcgagcc tctgtcgtag taggggtccg caagcatcgg ccagacgttc
     1501 ttctttaaat tcggcaggta gtcgtggttc aggggctgaa gcaacttccc cgccacgaac
     1561 ttcgggatga tctcggccgt ggggacgaag acgtcgaagt cgaccttgcc ggtcctcagc
     1621 ttctgggtgg cctcctccag gttgtagaag gtcgtgagct cgacctcgac gccgaactcc
     1681 tcttcgaaat ccttgagcac gcgggtgtag atgtaatccg accagttgta aaggcgcaaa
     1741 ggcccctttt ctggctccaa cccggagtcg atcatcgggt tgtcgtcgaa caggggctgc
     1801 ttgacggggt cttccctggt gccgacgacc acgcgcgaag cggacgtcga ggagccagcc
     1861 cccgagccgc aaccggctag catcccgcct gctgcagccg tccccgccat cagcctaagc
     1921 gcctcgcgac gcgacatgcg gcgcggccag ggagatcttt ccctggggtg gaatgccatc
     1981 tctatcaacc tcctattggg gcgtccggcc cagacagccc cgtccctacc cctcggccgc
     2041 gagctcctcc tgctcggcct ccctttcctt atcacgccgc ctcgacgcgt ccggctcgaa
     2101 gcccaactcc cgggccgccg cgtccagaca catctcgtag accagttctc cgttcaccgc
     2161 agggtcgccg aacaatacct ggagcgcgag gccgtccatt agcgcggaga ggtacgtcgc
     2221 gaagcgctcg gggtcgcact gcccgaattc gccggaccgc tggccctccc ttaccacctc
     2281 agctatggtg ttgcgccagc ggcgctcaag cgcctcgcgc ttgcgggccg cctcgtcgtt
     2341 gcgtaaggcc cgcgaccaca actcgaccca caacgtccag tactcgtcac tctcgcgccg
     2401 tacctccggc ggacaggcca gctcgatcaa ccgcaccagc cgacgcgaag catcctcgat
     2461 gcccgtcagc tcccggaaag cctccatata gaaaagatcc tccgcgtagg cgagcgcctc
     2521 ggtcagaagc gtctccttgg acccgaagta gtacaccacc agggccggac tcaaaccagc
     2581 cctctccgcc acatcagcga tacgcgtctc gcacagaccc cgctccgcga ttacctccat
     2641 ggccgcctcc agcatctgcg tgcggcgctt ctgcgtcaat ttgggcct
//
LOCUS       DATMJA010000312         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_811787, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000312 DATMJA010000000
VERSION     DATMJA010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811787"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VNT12_07475"
     CDS             <1..1057
                     /locus_tag="VNT12_07475"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWI45996.1"
                     /translation="AYGMVIKKPIGVCAAIVPYNFPLTLMGTKVGPALAAGNTIVVKP
                     ANTTPLATLRVAELMQEAELPPGVLNVVTGPGKEVGEALATHPDVRRVALTGSTQTGR
                     RMMETAAPKFKRVSAELGGSDPVIVFPDADVDAAVRGINIGRFFNAGQACLAAKRVYV
                     FEEIYDEFMEGLTKRVSRYELGDGMEKAEKPKIRMGPLNAARHRDVLADQLQDAVDGG
                     AKIAVGGNGHDGKGYFFVPTVIEDAPHDSRVVREETFGPLLPVFKVNDVDEAIRLAND
                     SAYGLGSSVYTHNVKWIHRAAQEIEAGMTWVNQLHFGYDELPFGGVKDSGIGREHGKE
                     AIEHYLEQKSVVVGGLD"
     gene            1139..1909
                     /locus_tag="VNT12_07480"
     CDS             1139..1909
                     /locus_tag="VNT12_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWI45997.1"
                     /translation="MAVEYSREGSVGYITLDNPPANSYDLSFMEELNECVRSAAEDKG
                     AGAVILRSGSERFFSAGADIKAFDANSVEDNMRMISQAHEGLSQIARTPKVFIAQIGA
                     TALGGGLEMALACDLRFGAEGEYVLGLPEATLGLLPGNGGTQRLPRLIGVSKALDLMV
                     TGRTISPSEAHELGILDRLFPADALEEETRKYAQKLAGGASEAIGSIKLAVYGGIERP
                     LDEGLQTERTLVEPLFDSEDAREGISAFMEKRRPEFSK"
     gene            1965..2600
                     /locus_tag="VNT12_07485"
     CDS             1965..2600
                     /locus_tag="VNT12_07485"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI45998.1"
                     /translation="MATSDTLIRASGREGEALAPKQARLIRSAYKVMGEKGLRHLSLQ
                     DVADEAGVSKAILPYYFESKENLILLTMRWVLARVAGRIREAIADASSAEEKVSAMID
                     AIFLSPESNRRFYLVFFDFLGYAARNDRFADVGATFHEICNGLYAEVINLGQEEGVFL
                     EVDSREGATVVRALVDGLFTQWIQEKEWGKTHAEYREMCKRSVLTYLTAGA"
     gene            2619..>3521
                     /locus_tag="VNT12_07490"
     CDS             2619..>3521
                     /locus_tag="VNT12_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI45999.1"
                     /translation="MSVAPSSIGTQPIGETSRVSDGIFQVKLPVPFPLKFVASYLVED
                     RDGWTVIDPGFDYGPARETWERCAAEVGLDLELDLARIIVTHLHPDHIGLARWLEERS
                     GAPVWMLEGEIENARRLWDPDRGTEDFVRYLMLNGMDEQTAGPTAGSTRLGVRLPERL
                     LPLQQGDSIELGSSETRVLHTPGHSDFHFILHDERRRLLFAGDHLLLEITPNIGLWPY
                     TAPRPLRRYLDSIAILLGLDVDLVFPGHGPLFHDLEGRVDELLAHHEERLSVMLAALD
                     GRQATPFEVARRVFPEDLTDHQLRF"
BASE COUNT          684 a         1084 c         1157 g          596 t
ORIGIN      
        1 ggcctacggc atggtcatca agaagccgat cggcgtgtgt gccgcgatcg tgccctacaa
       61 cttcccactc acgctgatgg ggaccaaggt cggaccggcg ctcgccgctg gcaacacgat
      121 cgtggtgaag ccggccaata ctaccccgct cgccacgcta cgagtcgccg agctgatgca
      181 ggaggccgag cttcctcccg gggttctgaa cgtcgtaaca ggccctggca aagaggttgg
      241 agaggcgctg gccacccacc ctgacgtcag aagggtcgcc ctcacgggca gcacgcagac
      301 ggggcggagg atgatggaga cagcggcccc caagttcaag cgcgtttcgg cggaactcgg
      361 tggatccgat cccgtgatcg tcttccccga cgcggacgtg gacgcggccg tgcgtggcat
      421 caacataggc cgatttttca acgcgggcca ggcttgcctc gccgccaagc gggtctacgt
      481 cttcgaagag atctacgacg agtttatgga gggccttacc aagagggtct cccgctacga
      541 actaggagac gggatggaga aggccgagaa gcccaagata aggatgggtc ccctcaacgc
      601 cgccaggcac cgtgacgtgc tggcagatca gctccaggac gcggtggacg ggggcgcgaa
      661 gatcgccgtc gggggtaatg ggcacgacgg caaaggctac ttcttcgtgc cgaccgtgat
      721 cgaggacgcc ccacacgaca gccgcgtggt gcgcgaggag actttcgggc cactgctgcc
      781 ggtcttcaag gtgaacgatg tggacgaggc gataaggctc gccaacgact cggcttatgg
      841 tctggggtcc tccgtctaca cgcataacgt gaagtggata caccgtgctg cccaggagat
      901 agaggcaggc atgacctggg tgaaccagct ccacttcggt tacgacgagc ttccgttcgg
      961 aggggtgaag gactcgggca taggccgcga gcatggcaag gaggccatcg aacactacct
     1021 ggagcagaag tccgtagtcg tcggaggact ggactgagag aggtatcagg tcgcggacct
     1081 ctgtagggtc cgcggcccca taccttcggc tagccgttcg agaactagag gaggacaaat
     1141 ggccgtcgag tattcgagag aaggcagcgt agggtacatc accctggaca acccgccggc
     1201 gaacagctac gacctatcgt tcatggagga gctaaacgag tgcgtgcgct ccgcggcaga
     1261 ggacaaaggg gccggagctg tgatcttgcg cagcggtagc gagaggttct tttccgcagg
     1321 ggcggacatc aaagcgtttg atgccaacag cgtcgaggac aatatgcgca tgatcagcca
     1381 ggctcacgag ggcctatccc agatagcccg caccccgaag gtgttcatcg cccagatcgg
     1441 ggcgaccgcc ctcggaggtg gactggagat ggccctggcc tgcgacctgc gcttcggtgc
     1501 cgagggcgag tacgtactcg ggctgcccga ggccaccctc ggcctgctcc caggcaacgg
     1561 cggtacccag cgcctgccgc gccttatcgg ggtgagcaaa gccctcgatc tcatggtgac
     1621 gggacggacg atctcaccct cagaggccca cgagctcggc atcctcgacc ggctgttccc
     1681 tgccgacgcg ctggaagagg agacccgcaa gtacgcacag aagctggcgg gcggcgcgag
     1741 cgaggccatt ggatccataa agctcgcggt atacggcggt atcgaaaggc cgctggacga
     1801 aggtctccag acggagcgca cgctcgtcga gccgcttttc gacagcgagg acgccagaga
     1861 aggcatctcc gcgttcatgg agaagcgaag gccggagttt agtaagtagg ctgtaacagt
     1921 ttgctccctt taggtacaat ccggcacatg gaggtgagag aaccgtggcc acatccgaca
     1981 ccctgatccg cgcctcaggc cgcgaaggcg aggcgctggc gccgaagcag gcacgcctga
     2041 tacgcagcgc ctacaaggtc atgggcgaga aagggttgcg ccacctctct ctgcaggacg
     2101 tggcggacga ggccggggtg agcaaggcca tcctacccta ctacttcgag tccaaggaga
     2161 acctgatcct cctcaccatg cgctgggtgc tcgcccgcgt cgcaggacgc atccgcgagg
     2221 cgatcgccga tgcgtcctcc gcggaggaga aggtctcggc gatgatcgac gccatcttct
     2281 tgagcccgga gtcaaaccgc aggttctacc tcgtcttctt cgacttcctg ggctacgcgg
     2341 cccgcaacga ccgcttcgct gacgtgggcg ccacgtttca cgaaatctgc aacggcctct
     2401 acgccgaggt catcaacctc gggcaagagg agggtgtctt cctcgaagtc gactctcgag
     2461 aaggcgcgac cgtggtgcgg gccctcgtgg atgggctgtt cacgcagtgg atccaggaga
     2521 aggagtgggg gaagacccac gcggagtacc gtgagatgtg caagcgctcc gtgctcacct
     2581 atctgaccgc cggtgcttag aggcgtgccc gtgatctcgt gagcgtcgct ccttcttcta
     2641 ttggcacaca gcccatcggc gagacgtcgc gtgtctcgga cggcatcttt caggtcaagc
     2701 tgcctgtgcc tttccccctg aagttcgtcg cgtcgtacct cgtcgaggac cgcgacgggt
     2761 ggacggtcat agaccccggc ttcgactatg ggccagcccg tgagacgtgg gagagatgcg
     2821 ccgctgaggt tggcctggat ctggagctcg accttgcacg gatcatcgtg acgcacctgc
     2881 acccggatca catagggctc gcccgctggc tggaagagcg tagcggggcg ccggtctgga
     2941 tgctcgaagg tgagatcgag aacgcccgcc gcctctggga tccggacagg ggaacggaag
     3001 actttgtacg gtacctgatg ctcaacggca tggacgagca gacggctgga ccgacggcag
     3061 gatcaacgcg gctaggcgtc cgcctgccgg agcgcctgct gccgctgcag cagggagact
     3121 cgatcgagct cggaagcagc gagaccagag tgttgcacac gccgggccac tcggattttc
     3181 acttcatcct gcacgacgag cgacggcggc tcctgttcgc cggagaccac ctcctgctcg
     3241 agatcactcc gaacatcggg ctgtggccgt ataccgcccc gcggccgctc cggcgttacc
     3301 tcgactctat tgctatcctg ctgggtctgg acgtagacct cgtcttcccc gggcacggtc
     3361 cgctctttca cgacctggaa gggcgcgtcg acgagctcct cgcgcaccat gaagagcgcc
     3421 tctcggtaat gctcgcagcc ctcgacggga ggcaggcgac gccattcgag gtcgcgcgcc
     3481 gcgttttccc ggaggatctc accgatcacc agctacgctt c
//
LOCUS       DATMJA010000313         3304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_219369, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000313 DATMJA010000000
VERSION     DATMJA010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219369"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VNT12_07495"
     CDS             <1..552
                     /locus_tag="VNT12_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWI46000.1"
                     /translation="LHSMRSSTVSRLYLQVPNGTDLADWPDDRIWDELATRLGHGQDG
                     WKLTPGPITEKSVLPMRSFVQAPMSYGRLFLAGDAAHIVPPTGAKGMNLAVADIRVLA
                     HALTEFYRSEDKGLLDAYSETCLRRVWRAQRFSWWMTSMVHRFDRSDPFQLKVQQAEL
                     DYVTTSRAAATTIAENYVGAALG"
     gene            593..1426
                     /locus_tag="VNT12_07500"
     CDS             593..1426
                     /locus_tag="VNT12_07500"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWI46001.1"
                     /translation="MSRGRISGKTGLLALVGQPVGHSLSPAMHNAAFAADGLDLVYVC
                     LDVDPDELPAAVRGLEALKLRGFNVTMPHKRAMIPLVDELDEEARISGAVNTVVIEAS
                     GLRGFNTDGGGMVMACQEAGIELSGKSVLLLGAGGTAAAIAVAFGKVGVGELHIANRS
                     VEHAAYLRDKLRGTGMTGLVVHPLDTLPDAEIVINATPLGMKEGDLMPVPPAYVREGR
                     GFCDAVYRPGTETPLVRLARERGVPVVAGDRMLLYQGVLAQKLWTGREPNVKAMDRAI
                     S"
     gene            1619..2077
                     /locus_tag="VNT12_07505"
     CDS             1619..2077
                     /locus_tag="VNT12_07505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWI46002.1"
                     /translation="MEESEQKIAERQEIRARYPHFLRVPTRWMDNDVYGHVNNVIYYS
                     YFDTVVNEYLIRSGVLDIERSPVIGLVVETQCLFFEPLTFPDTVHAGLRVARLGRSSV
                     RYEIGLFRNDGEMAAAQGHFVHVYVERESRKATPLPLGMRAALEKILASE"
     gene            complement(2081..2569)
                     /locus_tag="VNT12_07510"
     CDS             complement(2081..2569)
                     /locus_tag="VNT12_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWI46003.1"
                     /translation="MSEKTGLREDLRSEVEDLVASDAPDEEVLNQVVRRVHEAHPVWE
                     WSGIYLLVGDTLVLGPRTAPVDHSRIEIGEGVCGTAVSEDRNQVVEDVREVENYLACS
                     IHTRSELVVLIRNQGKIVGEFDIDSDTVGAFTQKDEALLDEMGLLISGRVASLAGSYE
                     SV"
     gene            complement(2735..2959)
                     /locus_tag="VNT12_07515"
     CDS             complement(2735..2959)
                     /locus_tag="VNT12_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46004.1"
                     /translation="MTEGQRDAPDRPEAERYYAQHASLDAGEAGSSEAALQEAINEGA
                     RQSWKLVGVTQDPTSGGVILVWDTSGFFSG"
BASE COUNT          665 a         1031 c         1004 g          604 t
ORIGIN      
        1 ctgcactcga tgcgttcgtc cacggtgagc cgcctctacc tgcaggtgcc gaacggcacc
       61 gacctggcgg actggcccga cgaccggatc tgggacgaac tcgcgacccg gctcggccac
      121 ggccaggatg gctggaagct caccccaggc ccgatcaccg aaaagagcgt gctgccgatg
      181 cgcagcttcg tacaggcccc gatgtcgtac gggcggctct tcctggccgg ggacgccgcg
      241 cacatcgtcc cgccgactgg agctaagggt atgaaccttg ccgtcgcaga tatacgggtc
      301 ctcgcccacg ccctgacgga gttctacaga tccgaggaca aggggcttct cgatgcgtac
      361 tccgagacct gcctgcgccg ggtgtggagg gcgcagcggt tctcctggtg gatgacctcc
      421 atggtgcacc gcttcgacag gagcgaccct ttccagctca aggtgcagca ggccgagctc
      481 gactacgtca cgacctcgcg ggccgcggca acgaccatcg ccgagaacta cgtcggagcg
      541 gccctgggtt gagcgatgag atcacaatct cccacccttt ataataggcg gcgtgtcgag
      601 agggaggatc agcggtaaga cagggttgct ggctctcgtc gggcaacccg tcggccacag
      661 tctgagcccg gctatgcaca acgcagcctt cgccgcggac ggcctggact tggtctacgt
      721 ctgcctggat gtagatcccg acgaactgcc ggcggcggtg aggggcctgg aggccctgaa
      781 gctcaggggc ttcaacgtta cgatgccgca caagcgggcg atgatcccgc tcgtggacga
      841 gttggacgag gaagctcgca tctcgggcgc tgtcaacacc gtggtgatcg aagcctccgg
      901 attgcgcggt ttcaacacgg acggaggcgg gatggtgatg gcctgccagg aggccggcat
      961 agaactctcg ggcaagagcg tcctgctcct cggtgccggg ggtaccgccg cggccatcgc
     1021 ggtcgccttc ggtaaggtcg gggtaggtga gttgcatatt gccaaccgga gcgtcgagca
     1081 cgccgcatac ctaagggaca agctgcgcgg gacgggcatg acaggtctag tagttcatcc
     1141 cctcgacacg ctccctgacg cggagatcgt tatcaacgca acccctctgg gaatgaagga
     1201 gggggacctt atgccggtac cacccgctta cgtgcgggag ggtagggggt tctgcgatgc
     1261 ggtctaccgt ccaggaaccg agacaccgct tgtccggctg gcccgcgagc ggggcgtccc
     1321 cgtcgtcgcc ggcgacagga tgttgctcta ccagggcgtt ctggcgcaga agctctggac
     1381 cggacgcgag ccgaacgtga aggccatgga ccgggctatc tcttgaagcc tgtggccgtg
     1441 gtttgcttgt aggggcgttt cgcgaaacgc ccctacatta aaatggatag acgactactc
     1501 caagcgcgga gttcacgccc gaacttctcg gtacccaaac aagatcggtt tcgacacacc
     1561 gccgacgagt ccccataatg agttaacatg cctcgtcaaa gcgaaggagc aagggcggat
     1621 ggaagagtcc gaacagaaga tcgcggaacg tcaggagatc agggcacgct acccccactt
     1681 cctgcgtgta ccgacgcgct ggatggacaa cgacgtctac gggcacgtca acaacgttat
     1741 ctattactcg tacttcgata cggttgtgaa cgagtatttg atccgctcgg gcgtactgga
     1801 catcgagagg agtccggtta tcgggctcgt cgtcgagacg cagtgcctgt tctttgagcc
     1861 cctgaccttc cccgacaccg tccacgcggg cctgcgggtg gcacgtctgg gacgcagcag
     1921 cgtgcgctac gagatcgggc tattcagaaa cgacggcgag atggccgctg ctcagggaca
     1981 tttcgtccac gtctacgtcg agcgcgagtc tcgcaaggcg accccgcttc cgctggggat
     2041 gcgagcggcg ctcgaaaaga tcctcgccag cgagtagccg ctagaccgat tcgtatgagc
     2101 cggccagcga ggcgacacgg cccgagatca ggagccccat ctcatcgagc agggcttcgt
     2161 ctttctgcgt gaaggctccc acggtatccg agtcgatatc gaactcgccg actatcttgc
     2221 cttggttcct gatcaggacg accagttcgg agcgggtgtg gatcgaacac gccaggtagt
     2281 tctcgacctc gcgcacgtcc tctacgacct ggttacggtc ctcggagacc gccgtgccgc
     2341 atacgccctc cccgatctca atgcgcgagt ggtccaccgg cgccgtgcgg ggaccaagca
     2401 cgagggtatc ccccacaagc agatagatgc cgctccactc ccacacagga tgcgcctcgt
     2461 gcaccctccg cacgacctgg ttcaatacct cctcgtccgg cgcgtccgag gccacgagat
     2521 cctctacctc ggatctcaag tcctcacgaa gccctgtctt ctcactcatc gatggagtcc
     2581 ttccgtaaat ggaacatcta ccaccaataa taacgccgcc cgggagagaa atccgtgaag
     2641 atcctaccga agggacctga tgacgcggtt tgcctgtagg agaggcgctt cgcgaagcgc
     2701 ccctacccaa actgcccatg tatgaagcgc cctgctagcc ggagaaaaac cctgacgtgt
     2761 cccacaccag aatgacgccg ccgctcgtag ggtcttgcgt tacgccgacc agcttccagc
     2821 tctggcgcgc tccttcgttg atcgcctcct gcaaggcagc ctccgaagag cctgcctccc
     2881 cggcgtccag tgaagcgtgc tgcgcatagt agcgctcggc ctccggacgg tcgggagcat
     2941 ctctctgtcc ttcagtcatg tccttcctcc atactttctg ttgccccttg ggcaaacttt
     3001 caacacaaac atgatgccat ccacacgagc cacgtgcacc caccagaggt ccagcagact
     3061 attgccgagc tgttattaca acattacagt tctctatcat ggcggtggga aggtaaatag
     3121 acggagcgca tactcgaaat gcacgaataa agtcgccgtg ctagactgcc cgcacctaaa
     3181 accgccgaat gtaagggaaa aatgcggcgg atgtaaatac gttgacagcg gcctggggaa
     3241 agccgctaga acgtgaattt cgggcgagtc gtacagacgt tgagtccgtt tcggggaaag
     3301 gaga
//
LOCUS       DATMJA010000314         4819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_811898, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000314 DATMJA010000000
VERSION     DATMJA010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811898"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VNT12_07520"
     CDS             complement(<1..822)
                     /locus_tag="VNT12_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWI46005.1"
                     /translation="MKSPHDRALRDIEEVFGDRLKRGGRPAPPGTPDTGEALASVSPL
                     SAEEVEFLVEAAGRYSVPLVALGAGTAPTPEPQGGSILVHFDLMRGLRLPEREEPWVE
                     AEPGASWLQLDDLLRGRGWGLAVYPTSAPRTTVGGWLTQDGLGVGSFEYGWLSENVLS
                     TSVVLPDGELVEVAGEVMQSFVGPGAAGIIVGAKLRTRRADADVLFGATFESASEMVG
                     AVTGIVEASMPLWHIAFLNPSMAHARNLGESFLLFGAYSAERAGGIEDGLRSVVAA"
     gene            complement(924..1667)
                     /locus_tag="VNT12_07525"
     CDS             complement(924..1667)
                     /locus_tag="VNT12_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWI46006.1"
                     /translation="MEPAGATTVFLFALLTAVATGFGAVPFAFAKHPTRRWLGVSNAV
                     AAGLMLAASFGLIYEGMNYGPLRALAGALLGLVFIVVARRFLRHEEHPAVFASMSGLD
                     ARKALLIVGVMTLHSFTEGVGIGVSFGGGEALGLFISLALAVHNIPEGLAISLVLVPR
                     GVGWDRAALWSIFSSLPQPLMAVPAFLFVEVFRPLLPYGLGFAAGAMIWMVFSELVPD
                     ALEEASSSLVAVVVTVSVVAMVAFQVFIR"
     gene            complement(1713..2201)
                     /locus_tag="VNT12_07530"
     CDS             complement(1713..2201)
                     /locus_tag="VNT12_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46007.1"
                     /translation="MAESEFVWRLGETGYGRINGRVGILDLLACAGLLVHEGLVLTRR
                     AHEEFLRTSGVLLDIEAARRGEDARRQAAEIRSRHTSYFVEGGLNLAICEALIGLNAR
                     AVIVLSADFAKSGLRSIPEVKDAVRDAWLSLRGLERQVEAAARGKDIPTWPLLVYSQA
                     KI"
     gene            complement(2401..3072)
                     /locus_tag="VNT12_07535"
     CDS             complement(2401..3072)
                     /locus_tag="VNT12_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWI46008.1"
                     /translation="MDEDRQLRELPEAVKNLIEARTGDAVYVVDPDYRIVHWDSRMES
                     LTGALSQEALGKPCYEAVMGEREDGGPFCAHGCSVMHLAQAHRPVSNFEMRIKTRSGD
                     RRWMNVSNLTVETEEGPYLVHLLRDFQGTHDALEMARGLIQLSSKEEAPAPKRKDIPA
                     LTPRQLEVLQLLSEGKSVKEIGKKLYLSEATIRNHVRSLLQTLGAHSQLEVLAKAREM
                     GILGG"
     gene            complement(3196..3651)
                     /locus_tag="VNT12_07540"
     CDS             complement(3196..3651)
                     /locus_tag="VNT12_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46009.1"
                     /translation="MNDIPEVALRTIEDTFGTRFVRHADGEAEPDAGQLFASVFPENA
                     SEVESLTKLAARHRIPLIARGAGTALNPGRDRPLRPAVRSYGARGLPQRSRSSSASLA
                     IQGTVSRWGEVALLAFDPAKGSADLVDLSATTDIELVRLAGRSWMAERR"
     gene            complement(3683..4285)
                     /locus_tag="VNT12_07545"
     CDS             complement(3683..4285)
                     /locus_tag="VNT12_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46010.1"
                     /translation="MMDIQLEVLLAVGYAIFLLGVAVGLDLLARHSHARSERYRTAGF
                     TYHPSHDAWVCPEDQLLLRSEVDHERRLVRYRGRPQICNYCPAKAECTDSDEGREVVR
                     PMDPWPHSEAGRFHRGISLAVALIAGLILAVEAIRNHAPFELAALGAVLAVVALVAWH
                     LHAAFRATPANFPAPRVAPSSKIPTDPVQMGYRKGKRVER"
     gene            complement(4287..>4819)
                     /locus_tag="VNT12_07550"
     CDS             complement(4287..>4819)
                     /locus_tag="VNT12_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWI46011.1"
                     /translation="WNFLGAGIFGFLINLPIISYYQIGTTLTANHGHAAMMGVYGMLA
                     AGLALFCLRYLIPEELWSERAAKISFWSLNIGLAWMCFATLFPLGILQLYQSLSVGYY
                     EARTLEFLTNETNALLEWMRLPGDVVFIVGGVLPLLYLCYLGVRHTVPRVVMEEPEDI
                     LFTELTEPEATGPRER"
BASE COUNT          896 a         1629 c         1474 g          820 t
ORIGIN      
        1 ggccgcgacg acgcttcgga gaccgtcctc gattcctccc gcccgttctg ccgaataggc
       61 tccaaagagc aaaaagctct ccccaaggtt tctggcgtgt gccatgctag ggttcaaaaa
      121 agcgatgtgc cagagcggca tgctcgcctc gacgatgccc gtcacggcgc cgaccatctc
      181 gctcgccgat tcgaaggtgg cgccgaagag cacgtccgcg tccgcccgcc gggtgcgcag
      241 cttcgcaccc acgataatcc ccgccgctcc gggtccgacg aaggattgca tcacctcccc
      301 cgctacctcc acgagttctc cgtccggcag caccacgctc gtcgacagaa cgttctcgct
      361 cagccaccca tactcgaagg accctactcc caggccatcc tgggtgagcc agccgccgac
      421 ggtggtccga ggggcgctcg tagggtagac ggcgaggccc catccccgcc cgcgcaggag
      481 gtcgtccagc tgcagccacg acgctcccgg ctcggcctcc acccaaggct cctcgcgctc
      541 cggtagccgc aggccacgca tcaggtcgaa gtggaccagt atgctcccgc cctgcggctc
      601 cggtgtgggg gccgtcccgg cgcccagggc gaccagcggc acggagtaac gtccggccgc
      661 ctccacgagg aactcgacct cctcggcgct cagcggcgac acggaagcta gagcctctcc
      721 agtgtccggt gttcccggcg gagccggtcg tcctccgcgc ttcaaccggt ctccgaagac
      781 ctcctcgatg tctctcaggg ctcgatcgtg gggagatttc atcgcctcca cctttctatt
      841 cggttacatt cggaacttac taccgcgtgg ggagcaattc ctgagcaatc ttgacggttt
      901 gtcccatcca tgttgatcaa tacttaccgg ataaagacct gaaaagccac catagcgacg
      961 acggagaccg tgaccaccac agccacgagg ctgctcgaag cctcctcgag ggcgtcgggc
     1021 acgagctcgg agaagaccat ccagatcatg gccccggccg cgaaacccag cccgtaaggc
     1081 agcagcggcc ggaagacctc gacgaagagg aacgccggca ccgccatcag gggttgcggc
     1141 aggctggaga agatgctcca cagcgcagcg cgatcccatc ccacgccgcg cggcacgagg
     1201 accaggctga tggcgagtcc ctctggtatg ttgtggacgg ccagggccag gcttatgaac
     1261 aggccaaggg cctcaccccc gccgaaagac acccctattc ccaccccttc ggtgaacgag
     1321 tgtaacgtca tgacgccgac gatgagcagc gccttgcggg cgtcgaggcc gctcatactc
     1381 gcgaagaccg ccgggtgctc ctcgtgccgg aggaatcggc gcgcgaccac gatgaacacc
     1441 agcccgagca gggcgcccgc cagcgcacgc aggggaccgt aattcatgcc ttcgtagatc
     1501 aggccgaagc tcgccgcgag catgagtccg gccgcgacgg cgttggagac cccgagccac
     1561 cggcgtgtag ggtgcttcgc gaaagcgaag ggcacggcgc caaagccggt ggccaccgcc
     1621 gtgagcagcg cgaagagaaa gaccgtagtc gcgcccgccg gttccatgcc cctacacccc
     1681 gaggatccgc cgtcgcccgc gagctcgcat tctcagatct tggcctgtga atagaccagc
     1741 aacggccagg taggaatatc cttcccgcgg gcggccgctt cgacttgcct ctccaaccct
     1801 cgaagggaga gccacgcatc ccgaacagcg tccttcacct cggggatgct cctcaaccca
     1861 ctcttcgcga aatctgcgga gaggacgatc accgccctag cgttcaaccc gatgagcgcc
     1921 tcgcagatcg ccaggttcag ccctccctct acgaaatacg aggtgtgtct cgatcgtatc
     1981 tccgcagcct gccgccgggc atcttctccc ctacgggcgg cctcgatgtc cagcaagacg
     2041 ccgctggtcc gcaagaactc ctcgtgcgcc cgccgggtga gcaccagccc ctcgtgaacg
     2101 aggagacctg cgcaggcgag taggtccaag atccccacgc gcccgttgat tcggccatac
     2161 cccgtctctc cgagacgcca gacgaactcg ctctccgcca tttgagattc cttccaactc
     2221 tgttgtctgc gtcactattc ccgcctccgc gagacgagcg ctctcatgaa gagtccgctc
     2281 gacctcgtcg gccgtcgcta taccgtaccg gacgcaaggc ggctggccat gaccagggtt
     2341 gcccatctgc ctagcaagga ttgaccaaac atggcgtcag tagacgcgaa gggtgagggt
     2401 ttagccgccg aggatgccca tctcgcgggc cttggccaac acctccagct gggagtgcgc
     2461 ccccaacgtc tgcaacaggg agcgcacgtg gtttctgatc gtggcctcgg acaggtagag
     2521 cttcttgccg atctccttca ccgacttgcc ctctgacagc aactgcagca cttcgagttg
     2581 cctcggggtg agggcgggaa tgtccttcct cttcggggct ggggcctcct ccttggaaga
     2641 aagctgtatg agcccgcggg ccatctctag ggcgtcgtga gtcccctgaa agtcgcgaag
     2701 aaggtgtacc agatacggtc cctcctcggt ctctaccgtg aggttgctga cattcatcca
     2761 tcgcctgtct cccgagcggg tctttatacg catctcgaag ttcgagacgg gacgatgggc
     2821 ctgggccagg tgcatcaccg agcaaccgtg ggcgcagaaa ggtcccccat cctcgcgctc
     2881 gcccatgacg gcttcgtaac aaggctttcc taaagcctcc tgggagagcg caccggtgag
     2941 agactccatc cgagaatccc agtggacgat cctgtagtcg ggatccacca cgtagacggc
     3001 gtcgcccgtg cgggcctcga taaggttctt caccgcctcg ggaagctctc gcagctgcct
     3061 atcctcgtcc atcacaggcc tattgtatag tccgcctccg gaggatcaat cgtcgcgagg
     3121 tcccgagcct atggaccttg agccgacaaa ttccttctta cctagctcag ggcctcgatt
     3181 ctcgaccact cccctctacc gccgctcggc catccaggag cggccggcca gccgcaccaa
     3241 ctcgatgtcc gtagtggcgg acaggtccac aagatccgcg ctccccttgg ccggatcgaa
     3301 agctagaagc gccacctcgc cccaccggga taccgttccc tgaatcgcca agctcgcgct
     3361 cgagctccgc gagcgttggg gtaagccgcg cgccccgtat gaacgcacgg ccgggcgtag
     3421 gggtcggtcc cttcccggat tgagggcggt gcctgcaccc cgggctatca gtggtatgcg
     3481 gtggcgggcg gcgagtttcg ttagcgactc gacctctgaa gcgttctctg ggaacacgga
     3541 ggcgaatagc tgcccggcat ccggttccgc ttcgccatcc gcgtgccgca cgaatcgggt
     3601 tccgaacgta tcttcgatgg ttctcagggc cacctcgggt atgtcgttca taagaggcct
     3661 ttctaccact cgtgcatcac gttcaacgct ccacccgttt gcccttcctg taacccatct
     3721 gaacgggatc ggtaggaatt ttagacgacg gggcgactcg cggagccggg aagttggccg
     3781 gggtggcgcg gaaggcggcg tgcaggtgcc aggccacaag cgcgacgacc gcgagcacgg
     3841 caccaagggc cgcgagttcg aatggagcgt ggttgcggat cgcctcaacc gcgaggatca
     3901 ggccggctat gagcgccacc gcgagcgaga tgccccggtg gaaacggccc gcctccgagt
     3961 gcggccaggg gtccatgggg cgcacgacct ccctcccctc gtcggagtcc gtgcactcgg
     4021 ccttggccgg gcagtagttg cagatctggg gcctgccacg gtagcgaacc agccgacgct
     4081 cgtgatccac ttcgctcctg aggaggagct ggtcctctgg gcagacccag gcgtcgtgcg
     4141 agggatggta ggtgaacccg gcggtgcggt agcgctccga gcgggcatga gagtggcggg
     4201 ccaaaaggtc gagcccgacg gcgactccaa gcaggaagat cgcgtatcct accgcgagga
     4261 ggacctcgag ttgaatatcc atcatgttac cgctccctcg gcccggtggc ttcgggctcg
     4321 gtgagctcgg tgaagagaat gtcctccggc tcctccatca ctacacgcgg aacggtgtgg
     4381 cgcacgccga ggtagcagag gtacaacagc ggaagcacgc cgcctacgat gaagaccacg
     4441 tcgcccggca ggcgcatcca ctccaggagg gcgttcgtct cgttggtcag gaactccagt
     4501 gtgcgggcct cgtagtagcc cacgctcagg gactggtaga gctgcaggat gcccaatggg
     4561 aagagcgtgg cgaagcacat ccaggcgagc ccgatgttga gcgaccagaa gcttattttg
     4621 gctgcccgct ccgaccagag ctcctccggg atcaggtacc gcaggcagaa cagggccaac
     4681 cccgccgcga gcatcccgta aacgcccatc atcgcggcgt ggccgtggtt ggcggtcagg
     4741 gtggtgccga tctggtagta ggagatgatc ggcaggttta tgaggaagcc gaagatcccc
     4801 gctccgagga agttccaga
//
LOCUS       DATMJA010000315         3482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_160504, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000315 DATMJA010000000
VERSION     DATMJA010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160504"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VNT12_07555"
     CDS             <1..997
                     /locus_tag="VNT12_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI46012.1"
                     /translation="LEEWTGVPATNIVQEEAERLLSLESMLHERVVGQEPAVSAVAEA
                     IRRARAGIKDPRRPVGSFIFLGPTGVGKTELARTLAEYLFGEEDAMIRIDMSEFQEGH
                     TVSRLVGAPPGYVGYEEAGQLTEQVRRRPYSVVLFDEIEKAHPDVFNTLLQVLDDGRL
                     TDAKGRTVNFENTVVIMTSNVGSQHLVSTRQFGFTSRDGVDFRETERRAREALERAFR
                     PEFLNRVDEIIVFQPLTKEDVLKIVDIMLSRLNKHLESQRVQVEVTDAAKEFLAEEGY
                     DPKFGGRPLARAIRRYVENPLSSRIIGGEFGPGDTVVVDRADEGLTFESKVPAAH"
     gene            1070..2041
                     /locus_tag="VNT12_07560"
     CDS             1070..2041
                     /locus_tag="VNT12_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI46013.1"
                     /translation="MMELPEGYTTRAPAREDAEVVADLISACQIADTGASDMSVEEML
                     DDWHSLDLAEEAVILTAPDGRIAAYADVFNRSFVIVSIYGYVHPDYREVGLGSYLVAW
                     GERWTRDHMPQAQENARVVAQHYINSANEAARLLLVNSGYTPVRGIYVMETTLDDPPP
                     LPHWPADISVRTFVPGRDERAVYEAVEDAFRDLRGRPRNPFERFVRETEKENFDPSLW
                     FLAVEGDEIAGLTLCKTLAGEGWVDVVAVRRPWRNRGLGLALLRHAFTEYHRQGTHRV
                     SLSVDAESITGAPRLYGRAGMRVRESYIIHLKEFRPGVDLGMRLDED"
     gene            2107..2754
                     /gene="lexA"
                     /locus_tag="VNT12_07565"
     CDS             2107..2754
                     /gene="lexA"
                     /locus_tag="VNT12_07565"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWI46014.1"
                     /translation="MGEALHPRRVEILRYLAGRASVEGPPPSVREIGAAVGLSSSQTV
                     HHHLGRLEEEGYVMRVGRGARTLGLTGKGWEAVGHMALLGRIAAGRGLEAVAFGDEAY
                     SLAAELLSVRSGRRRYLLRVVGQSMVGARIEDGDLLVVEEDEIPPDGAVVVALLGDGD
                     EVTVKRLYREGAKVRLRPENGGHKEILVPAEEVRVQGRVVYVVHPPRLGPSGDGS"
     gene            2761..3297
                     /locus_tag="VNT12_07570"
     CDS             2761..3297
                     /locus_tag="VNT12_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46015.1"
                     /translation="MGYVLDLLWGLLSYVAFTGAGGLVGYFFARKRTEHEVSYSRRVE
                     TVEVIQDSVASIVEELWAAREYVSGSGPFDGAPAKRIDRVLDEFEGYYVRREIWLNRE
                     TYARLESFTLTLRAYQRELASLPLYYEDPDFEREHERVGHELDRWLKTDLPAAREGLA
                     DAFRGMLGVGRWRRGDLR"
BASE COUNT          643 a         1002 c         1201 g          636 t
ORIGIN      
        1 cctcgaggag tggaccggcg tgccagcgac caacatcgtc caggaggagg ccgagcggct
       61 tctcagcctg gagtccatgt tgcacgagcg ggtcgtcggg caggagcccg cggtcagcgc
      121 ggtggcagag gccatcaggc gggccagggc cggcatcaag gaccctaggc gccctgtcgg
      181 cagcttcatc ttcctcgggc ctaccggggt tggtaagacc gagcttgcgc ggacgctcgc
      241 cgagtacctc ttcggcgagg aggacgccat gatccgcata gacatgtccg agttccaaga
      301 gggccacacg gtgagccgcc tcgtcggcgc gcctccaggc tacgtcggct acgaagaggc
      361 tggacagctc acggagcagg tgcggcgtcg tccctactcg gtcgttctct tcgacgagat
      421 cgagaaggcc caccctgacg ttttcaacac gctcctgcag gtgctcgacg acgggcgcct
      481 cactgacgcc aagggcagga cggtcaactt cgagaacacg gtcgttatca tgacctcgaa
      541 cgtgggcagc cagcacctcg tctccacgag gcagttcggc ttcacctcgc gcgatggcgt
      601 ggacttccgc gagaccgagc gccgggcgcg cgaggcgcta gagcgtgcct tcaggcccga
      661 gttcctgaac agggtagatg agatcatcgt cttccagccc ctcacaaagg aggacgtgct
      721 gaagatcgtg gacatcatgc tctcccgcct gaacaagcac ctcgaaagcc agcgcgtcca
      781 ggtcgaggtc accgacgccg cgaaggagtt cctcgccgag gagggctacg atccgaagtt
      841 cggcggacga ccgctggcgc gggccataag acgctacgtc gagaaccccc tctctagtcg
      901 catcatcggc ggcgagttcg gccccggcga caccgtcgtc gttgaccgcg cggatgaggg
      961 actaaccttc gagtcgaagg tgcctgcagc gcactagaac cacacacatc gtccgcgtga
     1021 ggagggcttc ccacataggg gagcccttct ctgtgttagg tttgtcgcca tgatggagtt
     1081 accggaaggc tacacgacga gggcgcccgc gcgcgaggac gcggaggtcg ttgctgatct
     1141 tatctcagcc tgccagatcg cagacaccgg cgcgtcggat atgagcgtgg aggagatgct
     1201 cgacgactgg cactctctcg atctcgctga ggaggccgtg atcttgaccg cgcccgacgg
     1261 ccgcatcgcc gcctacgccg acgtgttcaa ccgctccttt gtgatcgtct ccatctatgg
     1321 ttacgtccac ccggactatc gggaggtggg gcttggcagc tatcttgtcg cgtggggaga
     1381 gcgctggacg cgcgaccaca tgcctcaagc ccaggagaat gcacgcgtcg tcgcgcagca
     1441 ttacatcaat tcagccaacg aagcagcgcg actcctgctg gtgaactcag gctacacccc
     1501 ggtacgcggg atatacgtga tggagaccac cctcgatgat cccccgccac taccacactg
     1561 gcccgcagat atctccgtcc gtaccttcgt cccaggcagg gacgagcgtg cggtctacga
     1621 ggccgtcgag gatgcgttca gagacctgcg gggccgccct cgtaaccctt tcgagagatt
     1681 cgtcagggag acggagaagg agaacttcga cccatccctc tggtttctgg ccgtggaagg
     1741 cgacgagata gcaggactca ccctgtgcaa gacgctcgcc ggcgagggat gggtcgacgt
     1801 agtggcagta aggcgcccgt ggcggaaccg cgggttgggg ctagccctcc ttcgtcatgc
     1861 ctttaccgag taccaccggc aaggtaccca tagggtcagc ctcagcgtgg acgccgaaag
     1921 catcacaggc gctcccaggc tctacggaag ggccgggatg cgtgtcaggg agagctacat
     1981 catccacctc aaagagtttc gcccgggcgt cgatctcggc atgagactcg atgaagatta
     2041 gtctttatcc cacggcatca aacaaatgtt tgactaaggt actctcttgc gctagggtgt
     2101 gtatagatgg gagaggcatt acatccgagg cgggtggaga tcctacgcta ccttgcgggg
     2161 cgggcgagtg tggaggggcc tccgccgagt gtgcgtgaga tcggggctgc tgttggtttg
     2221 agtagttcgc agactgtaca ccaccacctt ggcaggctgg aggaagaagg atacgtgatg
     2281 agggtgggga gaggtgcgag gacgctcggg ctgacgggga agggttggga ggcggtgggc
     2341 cacatggctt tgcttggcag gatcgccgcc ggtcgtggcc tcgaggcggt ggcgttcggt
     2401 gacgaggcgt actcgctggc tgcggaactg ctctcggtgc gttccggacg gcggcgttat
     2461 ctcttgcggg ttgtggggca gagcatggtg ggggccagga tagaggatgg ggatctcctg
     2521 gtggtggaag aagacgagat tccgccggac ggcgcggtgg tcgtcgcgtt gctcggtgac
     2581 ggggatgagg taaccgtcaa gcggctctat cgcgaaggcg cgaaggtgcg gctgaggccg
     2641 gagaacgggg ggcacaaaga gatcctggtc cccgcggaag aagtaagggt acaggggagg
     2701 gtggtctatg tggtccatcc accgcggcta ggtccttccg gggacgggtc ttgagtccgg
     2761 atggggtatg ttctggacct gttgtggggt ctgctctcgt acgtggcatt cacgggcgcc
     2821 ggaggcctcg tggggtactt cttcgccagg aagcgcacag aacacgaggt gagctactct
     2881 cgtagggtag agaccgtcga ggtcatccag gactcggtcg cttccatagt cgaggagctc
     2941 tgggcggcac gcgagtatgt ctcggggagc ggccccttcg acggggcgcc tgccaagagg
     3001 atagatcgag ttctcgacga attcgagggg tactatgtgc ggcgtgagat ctggctgaac
     3061 cgtgagacct acgccaggct cgaatcattt accctcaccc ttcgcgccta ccagcgggag
     3121 ctcgcctccc tgccgctcta ctacgaagac cctgacttcg agcgggagca cgagcgcgta
     3181 ggccatgagc tcgaccggtg gctaaagaca gatctccccg ccgcccgcga agggctcgcc
     3241 gacgctttca gggggatgct cggggtcggg cgctggcggc ggggagatct gcggtaacct
     3301 ctgtaagaac atatcttgcg ggccacgtgg acgccgggta acatggcaat atacggggcg
     3361 tcccgtgcgg atagtccgat ggaaaggttc gtggagcggt gagcgcaacg ttatcggtgg
     3421 cggatgcgtc cgtggccgtg tactgcacgt tgctcgtggg ttggagtctc tcataggccg
     3481 gt
//
LOCUS       DATMJA010000316         4511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_693374, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000316 DATMJA010000000
VERSION     DATMJA010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693374"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VNT12_07575"
     CDS             <1..799
                     /locus_tag="VNT12_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWI46016.1"
                     /translation="VLGVPREASQAEIRNAYRNLAKEHHPDHPSGSAEEFSRLQEANA
                     VLSDPNRRRQHDEALDLAHAADQLAGLNLDFGSVEDELSAKRRERESGGPSLGERLRG
                     RFGRKEEDPYESETRDSGRGGGRRTRGRYDVSEARWYEPHRFDPEPVTLKSGAVSFVA
                     AFLAFIAIGQIGLWATAPEQVNPGPFAFVTILAPFMPILYSLVGLVVAYFAFRAAGYA
                     ALALTFVAVLVVGGQGPEGLLQFATVGIIVLLVVVYLGNRRESAARR"
     gene            827..2425
                     /locus_tag="VNT12_07580"
     CDS             827..2425
                     /locus_tag="VNT12_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HWI46017.1"
                     /translation="MTTTWELERFISDFERRMAPLERAVDEAWWNLATLGTDEAQEEF
                     VRAGKKYNGLFSDQNEYLTLRSWYENLEVLESPLLQRQVEVLYRTFAERQGDEEILGR
                     IEELEAEANAVYGNHRSLVRDREMGENEVRELLRTSADQDLRRETWEASKSVGRAVEE
                     TVRELARLRNRLAREQGYANHYARSLELQEIQAGELSKIMGSLESATDAPFRELKSDI
                     DTKVGQEFGVDAVMPWHLSGLFFQRATDIADIDLDRYFEEEDLEDLTRRTFDALGLDV
                     RGVISSSDLYERESKNQHAFCARLGREYPYDVRVLANVRSDAYWMDAMLHEFGHAVYD
                     RHVNPRLPYLLRTYAHANTTEAIALMMGALVDDPGWLRSVAGANRERLDQDSGKLAAH
                     RRADKMILTRFVLVMYHFEQALYADPDSADLNSVWWDLVERLLFVDRPPGRDEPDWAA
                     VIHVAVAPVYYHNYMLGELISAQLRNYLESHITQGPFYQNEVAGRYLLESFFGPGARE
                     NWRDTVLRATGEPLNPEYFVKSLG"
     gene            complement(2496..3467)
                     /locus_tag="VNT12_07585"
     CDS             complement(2496..3467)
                     /locus_tag="VNT12_07585"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HWI46018.1"
                     /translation="MTRRGLELRRTSALLLPAILAAALCAGCLNAVPEVQSVEASAGA
                     ASGGPAETSKENMSGAEGMPEATTIGASQIEGDLAGATDASSGVPFVAKTQASGGSGY
                     DADTVLAVRYKVNEDYERAVIDLGTGLEPASPVPEWSLVSQDGNGLLRVTLPSASATC
                     VSDGEFGDGLLESFHVVRAPEGGIFMDIFTRETFLYRVVELKDPARLVVDFKPKGDLK
                     APLPAEGGDTVLVEPRPSAKISDTLTVSGYSRNSEAAITIILLDSNGKVLMRRTVLSN
                     DGSHTWGYFEATLDLPSFSGKADLKVGTESAHNGSFEGTKIPVWASQ"
     gene            3589..>4511
                     /locus_tag="VNT12_07590"
     CDS             3589..>4511
                     /locus_tag="VNT12_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HWI46019.1"
                     /translation="MLVLTLGAEEHENMIGLRDIEAARRRLQGVALRTPLVPCPRGEQ
                     DRALYFKPESLQPTGAFKLRGAYNKISSLSPEDRRRGVVAHSSGNHAQAVAYAALALG
                     VKAAIVMPLGASRVKLDATAELGPEVVLVGPGSAERSRKAEELAEERGYIPVPPYDDE
                     VLIAGQGTIGAEILEDLPDVGTVLVPVSGGGLIGGISAAIKLARPEVRVIGVEPELAA
                     DARASLGSGRLVEFPADQVARTVADGLRVQKLGEAPFEHIRAFVDDIVAVTEEEILEA
                     MRRLALRVRLVAEPSGAVTFAAYLFHREELPT"
BASE COUNT          802 a         1449 c         1457 g          803 t
ORIGIN      
        1 ggtgctcggc gtcccacgcg aggcctccca ggccgagatc cggaacgctt accgcaacct
       61 ggcgaaggag catcaccctg accatcccag cgggagcgcg gaggaattct cccggctgca
      121 ggaggccaac gcggtcctct ccgacccgaa ccgtcgccgc cagcacgacg aagcgctcga
      181 cctggctcat gctgccgacc agcttgccgg cctcaacctg gacttcggct ccgttgagga
      241 cgagctctcc gccaagcgca gggagcgcga gtcaggcggt ccctccctgg gcgagcgcct
      301 gaggggccgc ttcggcagga aagaagaaga cccttacgag tccgagacgc gcgactccgg
      361 acgcggaggt gggcgcagga ccaggggccg ctacgacgtt agtgaggccc gctggtacga
      421 accccaccgc ttcgatcctg agcctgtgac cctcaagagc ggtgccgtaa gcttcgtcgc
      481 ggccttcctg gcgtttatcg cgatcggtca gatcgggctg tgggcaacgg cgccggagca
      541 ggtcaaccct ggtcccttcg ctttcgttac gatcctggct ccgttcatgc ccatcctgta
      601 ttcgctggtc gggctggtcg tggcttactt cgccttcagg gccgcgggtt atgcggcgct
      661 ggccctgacc ttcgtggccg tcctcgtcgt cggcggccag gggcccgagg ggcttttgca
      721 gttcgcgacc gtgggcatca tcgttcttct cgtagtggtt tacctcggca accgccgcga
      781 gagcgccgcc cggcgctgat cccgaccctt tagaaggagc tttttggtga caaccacctg
      841 ggaactagag cgcttcatct cggacttcga gaggcgcatg gctcccctgg agagggcggt
      901 ggacgaggct tggtggaacc tggctacttt ggggacggat gaggcgcagg aagagttcgt
      961 gcgcgccggc aagaagtaca acgggctctt ctcggatcag aacgagtacc tgactctccg
     1021 tagctggtac gagaacctgg aagtcctgga gagcccgctc ctgcaacgcc aggtcgaggt
     1081 gctctacagg actttcgcgg agcggcaggg ggacgaagag atcctgggcc gcatcgagga
     1141 attggaggcc gaggccaacg ccgtctacgg taaccaccgg agcctagtca gggacaggga
     1201 gatgggagag aacgaagtca gggagctgct gcgaaccagc gcagaccagg acctgcgccg
     1261 ggagacgtgg gaggcctcga agtccgtcgg ccgcgcggtc gaggagaccg tcagggagtt
     1321 ggcccgcctc aggaaccggc tggcccgcga gcaggggtac gcgaaccact acgcccgctc
     1381 gctggagctt caggagatcc aagccggaga gctctccaag ataatgggtt cgctcgagtc
     1441 agcgacagac gcaccgttca gggaactaaa gagcgacatc gacacgaagg tcgggcagga
     1501 gttcggggtg gacgccgtca tgccctggca cctctcaggc ctattcttcc aaagggccac
     1561 cgatatagcg gacatcgacc tcgaccgcta cttcgaggag gaggacctgg aggacctcac
     1621 ccgcagaacc ttcgacgccc tgggattgga tgtgcggggt gtgatctcat ctagcgacct
     1681 ctacgagagg gagagcaaga accagcacgc gttctgcgcc cgcctgggac gtgaataccc
     1741 gtacgacgtc agggtgctgg ccaacgtcag atccgacgcc tactggatgg acgcgatgct
     1801 ccacgagttc ggccacgctg tttatgatcg tcacgtcaac cctaggctcc cctacctcct
     1861 gcgtacctac gcccacgcca acacgaccga ggccatcgcc ctcatgatgg gcgcccttgt
     1921 cgacgacccc ggctggctcc gttccgtcgc cggcgcgaac cgagagaggc tggaccaaga
     1981 ctcaggcaaa ctcgccgccc accgccgggc cgacaagatg atcctcaccc gtttcgtgct
     2041 tgtcatgtac cacttcgagc aggcactcta cgccgaccct gatagtgcag acttgaactc
     2101 cgtgtggtgg gatctcgtcg agcggctgct gttcgtggac cgtccgccag gcagggacga
     2161 gcccgactgg gccgccgtca tccacgtcgc cgtagccccc gtctactacc acaactacat
     2221 gctcggggag ttgatctcgg cccagctacg gaattacttg gagtcccaca taacccaagg
     2281 ccccttctac caaaacgagg tcgcaggccg ctacctcctc gaatccttct tcggtcccgg
     2341 cgcccgcgag aactggcgcg ataccgtact acgcgccacc ggcgagccac taaaccccga
     2401 gtactttgtg aagtctctcg gctagtcagc acgctcaggc ttcgcttgtc agcttcattt
     2461 tctgcttggg cgagggccgg gctatcgctg accgcctact ggctggccca gacggggatt
     2521 ttggtgcctt cgaaggagcc gttgtgggcg ctttcggtcc cgactttcag gtccgccttt
     2581 ccagagaacg atggcagatc cagtgtggct tcgaagtatc cccaggtatg gctcccgtcg
     2641 ttgctcagca cggttctgcg catcaggacc tttccgttcg aatcgagcaa gatgatggtg
     2701 atcgcggctt cggagttgcg agagtagccg cttactgtga gggtgtcaga gatcttggcg
     2761 ctcgggcgtg gctcgacgag gaccgtgtca ccgccctccg cggggagcgg cgccttcagg
     2821 tctcccttcg gcttgaagtc cacgacgagg cgtgcggggt ccttcagctc gacgacccta
     2881 tagaggaacg tctcgcgcgt gaagatgtcc atgaatatgc ctccctcggg ggcgcgcacg
     2941 acgtggaaac tctcgagcaa cccatcgccg aattcgccat ccgatacgca cgtcgcgctg
     3001 gccgaaggca gggttacgcg cagtaggccg ttgccatcct ggctcacgag gctccactcc
     3061 ggcacgggac tggcgggctc cagtccggtc ccgaggtcga ttaccgcccg ctcgtagtcc
     3121 tcgttaacct tgtagcgcac cgcgagcact gtgtcggcat cgtagccgga accgccgctc
     3181 gcctgcgtct tcgcgacgaa aggaaccccg gaggaggcgt cagtagcccc agcgagatcg
     3241 ccctcgatct gcgaggcccc gatggtagtg gcctcgggca tgccctccgc acccgacatg
     3301 ttttctttcg aagtctccgc gggaccgccc gacgcggccc cggcagaagc ctctacggat
     3361 tggacctccg gaactgcatt caggcagcct gcgcagagcg cggccgccag gatcgcgggc
     3421 agcaagaggg cggaagtccg cctcagctcc aacccgcgtc gggtcatgct cgcacgtcca
     3481 catcataatg ttcggtcatc ggcgcgggcc attataggtg cgttcacctt gcgagatgtt
     3541 taacgcggtg ttgcgctcct gttacgatgc tggagtatcc accgtactat gctggtcctc
     3601 actctcggcg cagaggagca cgaaaacatg atcggattac gggatataga agccgcgcgg
     3661 aggcgtttgc agggagtggc gcttcgcacg cccctcgtgc cgtgtccgcg cggcgagcag
     3721 gacagggcgc tgtacttcaa gcccgagagc ctccagccta cgggcgcttt caagttgcgc
     3781 ggcgcctaca acaagatctc ctccctctcc cctgaggatc gccgccgcgg ggtcgtcgcc
     3841 cactctagcg ggaaccacgc tcaagccgta gcctacgcgg ccctggccct cggagtgaag
     3901 gccgctatcg tcatgccgct gggggcatct cgtgtgaagc tcgacgcgac ggcagagctc
     3961 gggcctgagg tcgttctggt cggccctggc agcgcagagc ggtctcgcaa ggcagaggag
     4021 cttgcggagg agcggggcta cattccggtg cctccatacg acgatgaggt tctcatagct
     4081 ggccagggaa ctatcggggc ggagatactc gaggacctgc ccgacgtggg gaccgtgctc
     4141 gtgcccgtca gcggtggcgg gctcatcggt gggatctcgg ccgccatcaa gttggcccgt
     4201 cctgaagtgc gagtgatcgg ggtggaaccc gagctcgcgg ccgacgcaag agccagcctg
     4261 gggagcggaa ggctcgtcga gttcccggcg gatcaggtcg cccgcacggt tgccgacggc
     4321 ttgcgcgtac aaaagctcgg cgaggcgccc ttcgagcata tccgcgcctt cgtcgacgac
     4381 atcgtcgccg taacggagga ggagatcctg gaagctatgc gccgcctggc gctgcgggtc
     4441 aggctggtcg cagagccgag cggggctgtg acgttcgcgg cctacctgtt ccacagagaa
     4501 gaactgccca c
//
LOCUS       DATMJA010000317         4613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_160767, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000317 DATMJA010000000
VERSION     DATMJA010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160767"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VNT12_07595"
     CDS             <1..376
                     /locus_tag="VNT12_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI46020.1"
                     /translation="DAVFLSAGGYHHHIGLNTWMSRNADPAPPHSAGLFHLAILYPER
                     QDLALALKWLLESNYPIDGASDHGVSEALYLQDPDGNGVELYRDRPQEQWPRTPEGGL
                     AMVTRPLDVEGLLAELDHEETS"
     gene            433..651
                     /locus_tag="VNT12_07600"
     CDS             433..651
                     /locus_tag="VNT12_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWI46021.1"
                     /translation="MGLEGVGKLLIGGAVALLVLGGALFLLGRFGVDRLPGDLVFRRG
                     NFTLYFPIGLMILLSVVGTIVINIVFRR"
     gene            653..1663
                     /gene="queA"
                     /locus_tag="VNT12_07605"
     CDS             653..1663
                     /gene="queA"
                     /locus_tag="VNT12_07605"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWI46022.1"
                     /translation="MLTSQLDYELPEDLIAQSPAEPRDASRLMVVDVRRGTIGHHTFR
                     DLPRFLLPGDALVLNETKVLPARLGAKKPTGGEVELLFLRDLGPDRGGVWEVLARPSK
                     RLRPGLGLLAGGDRLEIVESLGDGHWAVSAPDVWGLLERGGRMPLPPYIRPTPEAEGR
                     YQTVYARNEGSAAAPTAGFHFTEKVLEGAERAGATIGRITLHIGAGTFMPVRTKRLEE
                     HRMHAEHYSVPPEAARIIEGARRVVAAGTTVARTLETWNVTGEAEGESELFVYPGYRW
                     RSVDTLLTNFHLPRSTLLAMVMSFGGEDLVREAYMTAVRERYRFYSFGDAMLLLDGGR
                     QR"
     gene            1678..2799
                     /gene="tgt"
                     /locus_tag="VNT12_07610"
     CDS             1678..2799
                     /gene="tgt"
                     /locus_tag="VNT12_07610"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWI46023.1"
                     /translation="MEVNARDGEARAGVLSTPRGQVETPAFMPVGTKGTVKGLTPEDL
                     REAGAGIVLGNTYHLYLRPGSDLIKEAGGLHAFMGWYGPILTDSGGYQVFSLAQTRRI
                     SEEGVEFASVYDGSPHVFTPELTTRVQEDLGSDVAMVLDECPPAKAGREYHEESLRRT
                     GRWARRCKEAHGRRDQALFGIVQGGLYPDLREQSLGLTIEIGFDGYAVGGLSVGESRE
                     EMLRTLALTAPRLPAEKPRYFMGIGDPVGILKVIALGVDMFDCVLPTRLARHGAALTQ
                     DGRINLKNARYRTDFGPLDPGCACEACAGYSRAYLAHLVRENELLGHRLVTLHNVRFS
                     VDLCLRARREIGAGRYDEWSRAWISRYEDPDRAGSDKLE"
     gene            2812..4305
                     /gene="secD"
                     /locus_tag="VNT12_07615"
     CDS             2812..4305
                     /gene="secD"
                     /locus_tag="VNT12_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWI46024.1"
                     /translation="MSSRRNNLIVLSLVVVLLGVAAYFIFIHEPVTKSTQLGLDLQGG
                     VSAQLKGSQTGGGKVTRQEMEQSADLIRQRIDRLGVAEPDVRVQSENQIVVQIPGVKN
                     PDEVIRIIGSTAQLGFYQVLASDPSLTEPPQDVAADEVDKTEEDIRANLQDDDAYKVG
                     KTKILFEETPSSDGKGTSVIGYIVNKEPDLTGDALKQNGANVEFDQTTGKRIVSLELT
                     AEGGRQMGELTQNMLADAAASGEPARLAIALDQDIQSAPTVQEPLGRNISISNDGLPD
                     GLPEDEAKQLETVLNTGALPVNMEVISETTVGPTLGLSSLKSGLLASLVGFGLVLLLL
                     LVIYRALGVVADVALLIYAFLLWGLIVIIPITVTLPGIAGIVLSIGVAADANVVIFER
                     IKEEIRRGKTPRSAIQAGYDKGYRAILDGNITTLITAFILFALSSGSVRGFAVLLAIG
                     VVLSMFTAIVITRALLGLLSDRGIHVSPALMGVSKASISATQQARVR"
     gene            4308..>4613
                     /locus_tag="VNT12_07620"
     CDS             4308..>4613
                     /locus_tag="VNT12_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46025.1"
                     /translation="MFENIDFVGGWKLYFSLSTILLLAGAVAIALGGLNFGIDFQGGA
                     QFTVANAERQLSDEQVRAALPEELAEESVVTTLGQNGYEVRTPVISSQQEQSQVRNSL
                     "
BASE COUNT          861 a         1430 c         1499 g          823 t
ORIGIN      
        1 cgacgcggtc ttcctctccg cgggcggcta ccaccaccac atcggcctga acacctggat
       61 gagccgcaac gccgacccgg ccccgcctca ttctgcaggg ctcttccacc tcgccatcct
      121 ctaccccgag cgtcaggacc tcgcccttgc cctcaagtgg cttttggaat ccaactaccc
      181 catcgacgga gcctcagacc acggcgtctc cgaggctctg tacctgcagg atcccgacgg
      241 caacggcgtc gagctatacc gggaccgtcc gcaagagcag tggccgcgta cccccgaagg
      301 cggacttgct atggtcacaa ggcccctcga cgttgagggc ctcctcgctg agctcgacca
      361 cgaagagacc tcctgagcca acagccttgt cgtctgtgtg gacatcgggc gcggcgggct
      421 tagaatagga ccgtgggact cgaaggcgta ggaaagttgt tgatcggcgg cgcggtcgcg
      481 cttctcgtgc ttggcggagc gctctttttg ctcggtcgtt tcggggtaga ccggctgccc
      541 ggcgacctgg tattcaggcg gggtaacttc accctctatt tcccgatagg gctcatgatc
      601 ctgctctccg ttgtcggcac catcgtcatt aacatcgtct tccgccgcta gggtgctcac
      661 ttcccagctc gactacgagc ttcccgaaga tctcatagcc cagagcccgg cggagccacg
      721 cgacgcctcg cgcctgatgg tcgtagacgt ccggcgcgga acgatagggc accacacttt
      781 cagggacctg ccacgcttct tgctgccagg cgacgcgctc gtcctgaacg agacgaaagt
      841 gcttcccgcc aggctcgggg ccaaaaagcc gaccggtggt gaggtggaac tgctcttcct
      901 gcgagatctt gggccggatc gcggcggcgt gtgggaggtc ctggccaggc cgagcaaacg
      961 cctcagacca ggactcgggc tcctggccgg tggggatagg ctggagattg tcgagagcct
     1021 cggcgacggg cactgggccg tctctgcccc tgacgtatgg ggactgttag agcgcggcgg
     1081 taggatgccg ttgccgccct acatccgtcc aacgccagag gcagagggcc gctaccagac
     1141 cgtctacgcg cgcaatgagg gttccgccgc cgcaccaacg gccggcttcc actttacaga
     1201 gaaggttctg gaaggcgcag agcgggcagg agccacgatc ggcaggatca ccctccacat
     1261 tggagctggc acattcatgc ccgtgagaac caagaggctc gaggagcaca ggatgcacgc
     1321 cgaacactac agcgtgccgc cggaagcagc gcggatcata gagggtgccc gacgcgtcgt
     1381 cgcggcgggc actaccgtag cccgtacact cgagacgtgg aacgtgaccg gcgaagcgga
     1441 aggggagtcg gagctcttcg tctatcccgg ctaccgctgg cggtccgtag acacgttgct
     1501 gacgaacttc cacctgccgc gctccacgct tctggcgatg gttatgagct tcgggggcga
     1561 agatctggtc agagaggcgt acatgacagc ggtaagggag cgctaccgtt tctactcttt
     1621 cggcgacgcg atgctccttc tggacggcgg acggcagcgg taggcgcacc cgtctccata
     1681 gaggtaaacg cacgtgacgg tgaggcgcgc gccggtgtcc tgagtacgcc gcgcgggcag
     1741 gtggagacgc cagccttcat gccggtaggc acgaagggaa ccgtcaaggg actcacccct
     1801 gaagacctgc gggaggccgg ggccggcatc gtgcttggga acacgtatca tctctacttg
     1861 aggccaggat cggacctcat caaagaggcc ggtgggcttc acgccttcat gggctggtac
     1921 ggacctatcc tcacagactc gggcggctac caggtcttct cgctggccca gacgcggcgt
     1981 atctcggaag agggggtgga gttcgcgtcc gtgtacgacg gctcgccgca cgtgtttacc
     2041 cccgagttga cgacgagggt acaggaggac ctcggctcgg atgtggcgat ggtcctcgac
     2101 gagtgtccgc cggccaaggc gggccgcgaa taccacgagg agtcgctgcg gcggactggg
     2161 cgatgggcgc ggcggtgcaa ggaggcacac gggaggcgag atcaggcgct cttcgggatc
     2221 gtgcagggtg gtctttaccc cgacctgagg gaacagagcc tcgggctgac gatcgagatc
     2281 gggttcgacg gctacgctgt gggagggctc tcggtaggtg agtcgcggga ggagatgctg
     2341 aggacgttgg cgctaaccgc gccaaggctg cccgccgaga agccaaggta ctttatgggg
     2401 atcggggacc ccgtcgggat cttgaaggtc atagcgctag gggtagatat gttcgactgc
     2461 gtgcttccga cgcgtctggc ccgccacggc gcggccctga cccaggatgg ccgcatcaac
     2521 ctcaagaacg cgagataccg cacggacttc gggccgctag atcccgggtg cgcctgcgaa
     2581 gcgtgcgcag gttacagcag ggcctatctg gctcatctcg taagggagaa cgagctactt
     2641 ggccataggc tcgttacgtt gcacaacgtc cgctttagtg tagatctttg cctgagggcg
     2701 cggcgtgaga taggggcagg ccgctatgat gagtggtcgc gggcttggat ctcccgctac
     2761 gaagatcctg accgtgcggg gtcagataag ctagaatgat tctccgatct tatgagtagt
     2821 cgtcgaaaca acctgatcgt actctccctt gtagtcgtcc tactcggggt ggccgcctac
     2881 ttcatcttta tccatgagcc tgtgacgaag tccacccagc tcggcctcga cctccagggc
     2941 ggcgtcagcg cgcagctgaa gggttcccag acaggcgggg gcaaggtgac ccgccaggag
     3001 atggagcagt cggccgatct catccggcag cgcatagacc gcctcggcgt ggccgagccc
     3061 gacgtcaggg tgcagtcgga gaaccagatc gtggtccaga tccccggcgt gaagaacccc
     3121 gacgaggtga tccgcattat cggcagcacg gcccagctcg gcttctacca ggtcctcgcc
     3181 tctgacccct cgctgacgga gccgccccag gatgtcgccg ctgacgaggt ggacaagacc
     3241 gaagaagata tcagggcgaa cctccaggac gacgacgctt acaaagtggg gaagaccaag
     3301 atcctcttcg aagagacgcc gtcctcggac ggcaagggta cctcggttat cggctacatc
     3361 gtcaacaaag agcccgacct gacgggcgat gcgctcaagc agaacggggc taacgtggag
     3421 ttcgaccaga ccaccgggaa gcgcatcgtg agcctggagc tcacagcgga gggcggcagg
     3481 cagatggggg aactgaccca gaacatgctg gctgatgccg ccgcgagcgg cgagccggcg
     3541 cggctcgcca tcgcgctcga ccaggacatc cagagcgcgc ccaccgtgca ggaaccactg
     3601 ggccggaaca tctctatctc caacgacgga ctacctgatg gtcttcccga ggacgaggcc
     3661 aagcagctgg agaccgtgct caacacgggc gcgcttcccg tcaacatgga ggtgatctcc
     3721 gagaccacag taggtcctac gctaggcctt agttccctga agagcgggct tctggcctcg
     3781 ctcgtcgggt tcggactcgt gctcttgttg ctgctcgtga tatacagggc gctcggcgtc
     3841 gtcgccgatg tcgcgctgct catttacgcg tttctgctct ggggtctgat cgtgatcatc
     3901 cctatcacgg tgacgctgcc gggaatcgcc ggcatcgtgc tctccatcgg ggttgccgcc
     3961 gatgccaacg tcgtcatctt cgagcgtatc aaagaggaga taagacgcgg caagaccccg
     4021 cggagcgcga tccaggccgg ctacgacaag ggttacaggg cgattctgga cggtaacata
     4081 acgaccctca ttacggcctt catcctgttc gcactctcca gcggatcggt caggggcttc
     4141 gccgtgttgc tcgccatcgg ggtcgtgctc tcgatgttca cggcgatcgt gatcacacgc
     4201 gcactcctcg ggttgctctc tgaccgcggc atccacgtct cgccagccct gatgggggtc
     4261 tccaaggcaa gtatctccgc gacgcaacag gccagggtga ggtaggcgtg ttcgagaaca
     4321 tagacttcgt cggcggctgg aagctgtatt tctcgctctc aaccatcttg ctgcttgcag
     4381 gggccgtcgc catagcgctc ggcggcctca acttcgggat agacttccag ggcggggccc
     4441 agttcaccgt tgccaacgcc gagcgccagc tcagcgacga gcaggtacgc gctgcgctgc
     4501 cagaggaact tgcagaggaa tccgtcgtca cgaccctggg gcagaacggc tacgaggtgc
     4561 gaactccggt cataagctct cagcaggagc agagccaggt acgcaactcc ctg
//
LOCUS       DATMJA010000318         5580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_634466, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000318 DATMJA010000000
VERSION     DATMJA010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634466"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VNT12_07625"
     CDS             complement(<1..329)
                     /locus_tag="VNT12_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46026.1"
                     /translation="MALLVALLVSSACSSGDEGKGGNEDVIAGASKQRTMDLPPKHTE
                     TAGGIGDGVETGNLRFRIFEVRAKDRVYAMSGPGKRPVTRGNISSEYVAIDYLAKNLS
                     GSPLTTGA"
     gene            complement(398..1150)
                     /locus_tag="VNT12_07630"
     CDS             complement(398..1150)
                     /locus_tag="VNT12_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWI46027.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGALFGKLSKAISVAAREGGGDPE
                     MNPALGLAVQKAKDANMPNDNIQRAIDKGTGAGSDADTYERITYEGYAPGGVAVLVDV
                     LTDNRNRAASDVRYLFSKNGGKLGTTGSVAYLFERKGVILVPKDSTDEDELMEVALEA
                     GAEDVEEQENDYRVVTAPEDFTAVREALGGADIAYENAEITMEPQNSIDLDAGTARQT
                     LRLIDALEENDDVQEVYANFDISEEVMAEVVS"
     gene            complement(1147..1755)
                     /gene="pdxT"
                     /locus_tag="VNT12_07635"
     CDS             complement(1147..1755)
                     /gene="pdxT"
                     /locus_tag="VNT12_07635"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWI46028.1"
                     /translation="MAGNGVAQGEKGQKKIGVLALQGDFREHAEILRGLGVEPVEVRA
                     VEDLEGLAGIIVPGGESTTIGKMMVSSGLLDGIRSYYYKGGPVWGTCAGMVLAASATT
                     GPRQPLLGLMNALVERNGFGRQVHSFEKDLEVDGFDEPFTGVFIRAPFFEDVGPGVEV
                     LSRIGDRVVAARGENILVTAFHPELTDDVRFHEYFLREVCSI"
     gene            complement(1745..2641)
                     /gene="pdxS"
                     /locus_tag="VNT12_07640"
     CDS             complement(1745..2641)
                     /gene="pdxS"
                     /locus_tag="VNT12_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWI46029.1"
                     /translation="MAEYGQLTGTFRVKSGMAQMLKGGVIMDVVDAEQARIAEEAGAV
                     AVMALERVPADIRADGGVARMSDPEKIVEIQESVTIPVMAKARIGHFVEAQILEALEV
                     DYIDESEVLTPADEKHHIDKAVFDVPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGN
                     VVEAVRHMRAIVGGIRRLGALDHEEMMTEAKNLGAPYELVRWVAENGRLPVVLFTAGG
                     IATPADAALMMQLGADGVFVGSGIFKSGDPALRARAIVKATTHFKDAKAVADASRGLG
                     VAMMGREMGDLAEGERLATRGW"
     gene            complement(2688..2891)
                     /locus_tag="VNT12_07645"
     CDS             complement(2688..2891)
                     /locus_tag="VNT12_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46030.1"
                     /translation="MRGRALLQRVGEFARKEVTLALPAAQRAASHGVQAFLKKFSEEY
                     ANERKKLKSGTGARSRFGGQGRR"
     gene            complement(2924..3127)
                     /locus_tag="VNT12_07650"
     CDS             complement(2924..3127)
                     /locus_tag="VNT12_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46031.1"
                     /translation="MAEEKRRKGTLLGVIAYVLMPGERRVRAAGHFRKAGFETLKGVG
                     ELVRPEKSQASSGPASRERIEIE"
     gene            complement(3120..4289)
                     /locus_tag="VNT12_07655"
     CDS             complement(3120..4289)
                     /locus_tag="VNT12_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HWI46032.1"
                     /translation="MRTILYTGKGGVGKTSVAAATALQASLAGKKVLVMSTDPAHSLA
                     DVFDTEIGQDPKEMASGLWAQEMDHTSMIEENWSAIQGYMTTLFEWQGADTLAAEELA
                     MLPGMDELFGLLMVRRHNQEGRYDALILDAAPTGETLKLLSLPDHMNWYVEKIFPIQR
                     RAAKLVRPFASRARSLPPLPEDSVFAAGQRFYEAIASVEEILTDRESASVRLVLNAEK
                     MVIAEARRAYTYLNLYDYGVDALVVNRLLPESVSDPYFARWHVAQARHMRTIEESFDP
                     IPILTARLFDREMFGLEALGALAEDVFEDAEPLGVLFRGATHDIVKNGDGYDVVFHLP
                     LAQKRNVDLSKKGAELLVKVGNYRRSILLPDALARLPAAGASFEEGSLKVRLKDG"
     gene            complement(4291..5160)
                     /locus_tag="VNT12_07660"
     CDS             complement(4291..5160)
                     /locus_tag="VNT12_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWI46033.1"
                     /translation="MDHRFSGDERREPIFRSDLEGPRPEPRYEPLRPEGGLWSVIKRW
                     VAPLAAVGLLLAKFKGLALLLLKVKFLGTALTMLVSIGAYALLFPVWFAVGIVVLIWV
                     HEMGHVLQLRREGIPASAPMFIPFLGAFVAMKQMPKDALAEARVGLAGPVLGTLGGLA
                     ALGLYAATREPLFLGLAYFNFIINLFNLAPLLPLDGGRAVGAMSLVFQLLGLVVMVAL
                     FFVAPIMAFIALLGLPELFHRWKTRNTPEGREYYDLPLRQRIGVGMVYLGLIAVLGLL
                     TGATFVPDPAALQ"
     gene            complement(5186..>5580)
                     /locus_tag="VNT12_07665"
     CDS             complement(5186..>5580)
                     /locus_tag="VNT12_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWI46034.1"
                     /translation="PVHNDEIDHQFVERQRERLLGVRDELLRIREGVAKDERDLGEKE
                     GDFTEHDFGDMSQDMFTREMDASIWEQAERRLGEVDRALQKIDEGTYGLSDDSGEPIP
                     RGRLEAVPEAIRTVEEQQRFEQERRPPI"
BASE COUNT          964 a         1922 c         1584 g         1110 t
ORIGIN      
        1 gcccccgtgg ttagtggtga gcctgagagg ttctttgcca gataatctat agcgacgtat
       61 tcgctggaga tgttcccccg cgttacgggt ctctttccag gccctgacat ggcgtagacg
      121 cggtctttgg cccgcacctc gaagatccgg aaacgcaggt ttccagtctc cacgccgtcg
      181 cctatccctc cagcggtttc cgtatgtttc ggtgggagat ccatggtgcg ctgtttggag
      241 gcgccggcga tcacatcctc atttccaccc ttcccctcgt caccggaaga gcaggccgaa
      301 ctcactagca gcgccacgag cagggccacg aggatcttct tgatggaggg tcccagtccg
      361 acccggccag agaaagtgcc ggcgaaagca ggacgcccta cgaaaccacc tccgccatga
      421 cctcctcgga gatgtcgaag ttcgcgtaga cttcctgcac gtcgtcgttc tcttcgaggg
      481 cgtcgatcag gcgcaacgtc tgcctcgccg tcccagcgtc gaggtcgatg ctgttctggg
      541 gctccatggt gatctccgcg ttctcgtacg cgatgtccgc tccaccgaga gcctccctga
      601 cggccgtaaa gtcctccggt gccgtcacga ccctgtagtc gttctcctgt tcttctacgt
      661 cttcggcccc ggcttccagg gccacctcca tcaattcgtc ctcgtctgtg gagtctttgg
      721 ggaccaggat cacgcccttc cgctcgaaga ggtacgcgac cgagccggtg gtcccgagct
      781 tgccgccgtt cttggagaag aggtagcgca cgtcggaggc ggccctgttg cggttatcag
      841 tgagcacgtc gaccaggacg gcgaccccgc ccggcgcgta gccctcgtag gtgatccgct
      901 cgtatgtgtc agcgtcggag ccggcgcctg tacccttgtc aatggcgcgc tggatgttgt
      961 cgttgggcat gttggcatcc ttcgcctttt gtacggcgag gccgagcgcg gggttcatct
     1021 cggggtcgcc gccgccctct cgagccgcga cgcttatagc cttggacaac ttgccgaaga
     1081 gcgccccgcg cttcgcgtcg gcggcacctt tcttgcgctt gatcgtcgac cacttactat
     1141 gaccgctcat atagaacaaa cctcccgcaa gaagtactcg tgaaacctga catcatccgt
     1201 aagctcggga tggaacgccg ttaccagtat gttctcgccc ctggccgcca cgacccgatc
     1261 tccgatcctg gatagcacct cgacccctgg ccccacgtcc tcgaagaacg gcgcccgtat
     1321 gaagacgccc gtgaacggct cgtcgaagcc atctacttcg aggtccttct cgaaagaatg
     1381 gacctgccgc ccgaacccgt tgcgctcgac gagcgcgttc atcaggccga gcagcggttg
     1441 gcgcggaccc gtcgtcgccg acgcggcgag gaccatcccg gcgcacgtgc cccacacagg
     1501 accacccttg tagtagtagc tcctgatgcc gtcaaggaga ccagaggaga ccatcatctt
     1561 ccctatcgtc gtcgactctc cgccaggaac gatgatcccg gccaggcctt cgaggtcctc
     1621 cacggcccgc acctcgacag gctcgacccc aagcccacgc aggatctcgg cgtgctccct
     1681 gaagtctccc tgcaaggcga gcaccccgat cttcttctgg cctttttctc cctgcgccac
     1741 gccgttacca gccacgggtc gcgagccgct ctccttctgc caaatcaccc atctccctgc
     1801 ccatcatcgc cactccgagt cccctgctcg catcggccac ggccttcgcg tctttgaaat
     1861 gggtcgtagc cttcacgatg gcccgagccc tgagggcagg gtcaccactc ttgaagatgc
     1921 cgctccctac gaacacccca tcggccccga gctgcatcat cagggccgcg tccgctgggg
     1981 tggctatgcc gccggccgtg aacagcacga caggaagccg cccattctca gcaacccatc
     2041 gcacgagctc gtatggagcc ccgaggttct tcgcctccgt catcatctcc tcgtgatcga
     2101 gggcaccgag cctgcgaatg ccgccgacta tggcgcgcat gtgccgcacg gcctccacca
     2161 cgttgcccgt gcctgcctct cccttggaac ggatcatggc cgccccctcg ccgatgcgcc
     2221 gcagcgcctc tccgaggttc gtagcgccac acacgaatgg cacgtcgaag acggccttgt
     2281 ctatgtgatg cttctcgtca gcgggagtca ggacctcgga ctcatctatg tagtcgactt
     2341 cgagagcctc cagaatctgg gcctccacga agtgcccgat gcgtgccttc gccatcacgg
     2401 ggatggtgac ggactcctgg atctcgacga tcttctccgg gtcgctcatc ctcgcgaccc
     2461 cgccgtcggc ccttatgtca gcgggtacgc gctccagggc catgacggcc acagcccccg
     2521 cctcctcggc gatcctggcc tgctcggcgt cgacgacgtc catgatgaca ccgcccttga
     2581 gcatctgggc catcccgctc ttgaccctga aggtccctgt taactgtccg tactcggcca
     2641 tataattctc ccgttggcat ctactagaat ttgtaaagga tatcttacta ccggcgcccc
     2701 tgaccaccaa accgggaccg tgcgccggta ccacttttca gcttcttccg ctcgttcgca
     2761 tactcttcgg agaacttctt caagaacgcc tgtacgccgt ggctcgcggc ccgctgcgcc
     2821 gcgggtaggg ccagggtaac ttctttcctg gcgaactccc ccaccctttg caacaatgcc
     2881 cgtcctcgca ctactctctc ctgctgccca tcaccggtct gccctactcg atctcgatac
     2941 gttccctgct cgcagggccg gatgaggcct gcgacttctc gggcctcacc agctctccga
     3001 cgcccttcag cgtctcgaag cccgctttgc ggaagtgccc ggcggcacgc acacgccgct
     3061 cgccgggcat gaggacgtag gcgatcacac ccagcagcgt gcccttgcgt cgcttctctt
     3121 cagccatcct tcaacctcac tttcagcgac ccttcctcga agctcgcccc agccgcaggg
     3181 agacgcgcga gcgcatccgg cagaaggatg ctcctccggt agttgccgac ctttactagc
     3241 aattcggccc ccttcttcga tagatccacg ttccgcttct gggcaagcgg gaggtggaag
     3301 acgacgtcgt agccgtcgcc gttctttact atgtcgtgcg ttgcacccct gaatagtacc
     3361 cccagtggct ccgcgtcctc aaagacgtcc tcggcgagcg cgccgagcgc ctcgagtccg
     3421 aacatctcgc ggtcgaagag ccgcgcggtc aggatgggga tagggtcgaa agactcctcg
     3481 atcgtcctca tgtgccgcgc ctgcgcaacg tgccagcgcg cgaagtaagg gtcgctcacg
     3541 ctctcgggca ggagccggtt gacaacgagg gcgtccacgc cgtagtcgta gaggttgagg
     3601 taggtgtagg cgcggcgggc ctctgctatg accatcttct cggcgttgag caccaggcgc
     3661 acagacgcgc tttcgcggtc ggtcaggatc tcttcgacgc tcgctatggc ctcgtagaac
     3721 ctctgccccg cggcgaagac gctgtcttcg ggcagcggcg gcagggacct cgccctgctc
     3781 gcaaacggcc tgaccagctt cgcggcgcgg cgctgtatgg ggaagatctt ctcgacgtac
     3841 cagttcatgt ggtcgggaag gctgaggagt ttcagcgtct cgcccgtagg cgcagcgtcg
     3901 aggatcaggg cgtcgtagcg accctcctga ttgtgtcgcc tgaccatcag gagcccaaac
     3961 agctcgtcca tcccaggcaa catcgccagc tcctcggccg cgagtgtatc tgcgccctgc
     4021 cactcgaaca gcgtggtcat gtacccctgt atcgcggacc agttctcctc gatcatggaa
     4081 gtgtggtcca tctcctgcgc ccacaggccc gaggccatct ccttggggtc ttgccctatc
     4141 tcagtgtcaa agacgtcggc gagcgagtgc gcggggtcgg tgctcatcac gagcaccttc
     4201 tttcccgcaa gcgaagcctg cagggccgtt gctgcggcga cgctagtctt gccgacgccg
     4261 cccttgcctg tgtagagaat cgtacgcaag ctactggagc gccgccggat ccggcacgaa
     4321 agtagccccc gtcagcaatc cgagtaccgc gatcaaaccc aggtagacca tgccaacccc
     4381 gatgcgctgg cgcagtggaa gatcgtagta ctcccgtcct tccggcgtgt tgcgcgtctt
     4441 ccagcggtgg aagagctcag gcaggccgag cagcgcaata aaggccatga tcggggccac
     4501 gaagaacagc gcgaccatca cgacgagccc gagcaactgg aagaccaaag acatcgcccc
     4561 gacggccctg cccccgtcga gcgggagcag cggcgcgagg ttgaacaggt tgataatgaa
     4621 gttgaagtat gcgagcccca ggaacagcgg ctcccgtgtg gcggcgtaga gcccgagggc
     4681 cgctagcccc cctagcgtac cgagcacagg accggcgagc ccgacgcgtg cctcggccag
     4741 ggcatctttg ggcatctgct tcatcgctac gaatgccccg agaaatggta tgaacatcgg
     4801 ggctgaagcc gggattccct cgcgtcttag ctgcaggacg tgacccatct cgtgcaccca
     4861 gatcaggacg actataccta ctgcgaacca gacgggaaac agcagagcat aagcccctat
     4921 gctgacgagc atggtcagtg ccgtgccgag aaacttcacc tttagaagga gaagcgcaag
     4981 gcccttgaac ttcgcgagca gaagccctac cgcggcgagc ggcgcaaccc agcgcttgat
     5041 gacgctccac aggccgcctt ctggcctcag gggctcatac ctgggttctg gtctcggccc
     5101 ttcgaggtcg ctgcggaaga tgggctctct tctctcgtcg cctgaaaaac gatgatccat
     5161 agacagatta taccgccggg tgaccctaga tcgggggtcg tcgctcctgc tcgaagcgct
     5221 gctgttcctc gacggttctt atcgcttcgg gtacggcctc gagcctgccc cgaggtatgg
     5281 gctccccact gtcgtcggag agcccgtagg taccctcatc aatcttctgc agcgcccggt
     5341 cgacttcccc gaggcggcgc tcggcctgct cccatatgga ggcgtccatc tcccgggtga
     5401 acatgtcttg gctcatgtcg ccgaaatcat gctccgtaaa atcgccctcc ttttcgccca
     5461 gatctcgctc gtccttggcc acgccttcgc gtatgcgcaa cagttcgtcg cgcacgccta
     5521 atagcctctc tcgctggcgc tctacgaact gatggtctat ctcatcgttg tgcacgggtt
//
LOCUS       DATMJA010000319         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_871555, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000319 DATMJA010000000
VERSION     DATMJA010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871555"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1420
                     /gene="glmU"
                     /locus_tag="VNT12_07670"
     CDS             11..1420
                     /gene="glmU"
                     /locus_tag="VNT12_07670"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWI46035.1"
                     /translation="MSESSPIFAVILAAGKGTRMKSNRAKVLHTLCGVPMVNYVIEAT
                     RPLVPEGLFVVVGHQAELVEAVLPEDAKPVLQEEQRGTGDAVRVALDALEGEDESVLL
                     VVNGDGPLISDRTLGELVERHSSAGVGATVLVAELEDPSGLGRVVEDAGVVRIVEERD
                     ATESERQIRVVNLGIYAFRLSEIRDTIGGLTSENQGGELYLTDVLETIGRRSRAVTYR
                     LKNPEEANLVNDRSQLARAEDILRRRILDAHMRDGVTVRDPASTHIEASVEIGRDTVI
                     LPGSLLRGETRIGTDCVIGPSTDLQDTRVGDGALVEHSVGRGAEVGDGASVGPYSFLR
                     PATVLDPGAKVGAYCEVKNSRVGAGSKVPHLSYVGDAEIGADANLGAGTITANYDGEN
                     KYRTLVGDGVFTGVNTNLIAPVTVGEDAYTGAGSVVNKDVPAGKLAVGAPARAIRESP
                     QRRRKRAQQGELAKNETEG"
     gene            1428..2423
                     /locus_tag="VNT12_07675"
     CDS             1428..2423
                     /locus_tag="VNT12_07675"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWI46036.1"
                     /translation="MMQNGYLTQTTEKRLMIVSGQGYPELSERIAERLDLELSGVELR
                     AFPNGELYARYLESVRGADMFIVQSLCDPVNENLMQLLIMIDAAKRASAKRIVAVIPW
                     YAYSRQDRKTKPREPITARLIANMIQAAGAERVMTMDLHSGQIEGYFSFPVDHLTAMH
                     TFVDYFVDQGFREAEDAVVVAPDTGEVKVAKQLAGHLRLPWAIVNKMRDRPGHSEVTH
                     VIGNVAGKRVVMIDDVIATGGTLVNAAKRLVEQGALEVSAAATHGEFSGDAYRKIEDS
                     PLKEVVVTNTLPLKEDEPRSKIQTLNIAPILASTIKNVFTDESVSEVFMGENQLF"
BASE COUNT          512 a          726 c          869 g          416 t
ORIGIN      
        1 gtgcgagtac atgtctgaga gttcgccgat cttcgccgtg atcctggcgg ctggcaaggg
       61 caccaggatg aagtcgaacc gggcgaaggt tctgcacacc ctgtgcggtg tcccgatggt
      121 caactacgtg atcgaagcca ccaggcccct cgtccctgag gggctattcg tcgttgtagg
      181 ccatcaggct gagcttgtcg aggcggtcct gcccgaggac gcgaagcctg ttttgcagga
      241 ggagcagcgc gggaccggtg atgccgtgcg tgtcgccctg gacgcgctcg aaggggaaga
      301 cgaaagcgtc ctcctcgtgg ttaacggcga cggtcccctt atctctgaca ggaccctcgg
      361 agaactggtc gaacggcaca gttccgccgg cgttggggcg acagtcctcg tggccgagct
      421 ggaggacccg agcggcctcg ggagggtagt cgaggatgcc ggcgtggtca ggatagtcga
      481 ggagagggac gcgaccgagt ccgagcgaca gatcagggtc gtcaacctcg gcatctacgc
      541 tttcaggctc tccgagataa gggatacgat cgggggtctc acctcggaga atcagggggg
      601 tgagctgtac ctgacagacg tactcgagac gatcgggcgg cgcagccggg cggtcactta
      661 caggctcaag aaccctgagg aggcgaacct cgtcaacgac cgctcccagc ttgcgcgggc
      721 cgaggatatt ctcaggcgcc gcatcctgga cgcccacatg agggacgggg tgacggtgag
      781 ggatccagct tctacgcata tagaggcctc ggtcgagatc gggcgcgaca ccgtgatcct
      841 gcctggaagc ctgttgcgcg gtgagacgag gatcggtacg gactgcgtga tcgggccttc
      901 caccgacctc caggacacac gtgttggtga cggcgctctc gtcgaacact cggtcggacg
      961 cggggccgag gtcggcgacg gggcgagcgt cggtccctac tctttcctgc ggcccgctac
     1021 ggtcctcgac ccaggcgcca aggtcggggc ttattgcgag gtcaagaact cgcgggtagg
     1081 cgcggggagc aaggtccctc acctctcgta cgtgggggac gcggagatcg gggccgacgc
     1141 caaccttggg gccgggacca taacggcaaa ctacgacggc gagaacaagt accgtacctt
     1201 ggtgggagac ggtgtcttta caggggtaaa tacgaaccta atcgcgcccg ttacggtggg
     1261 cgaggacgct tacactgggg ctgggtcggt ggttaacaaa gacgttccag cgggcaaact
     1321 ggccgtgggg gcaccggcgc gtgcgatccg ggagtctccc cagcggcgta ggaagcgggc
     1381 gcagcaaggc gagttggcca agaacgagac ggagggctag cgcgaggatg atgcagaacg
     1441 gctatctgac gcagaccacg gagaagcggc tgatgatagt cagcggccag ggctatcccg
     1501 agctatccga gcgtatcgcc gagcggctcg acctcgagtt gagcggggtc gagctgaggg
     1561 cgttccccaa cggcgaactg tacgccaggt atctggagag cgtcagggga gctgacatgt
     1621 tcatcgtgca gtcgttgtgc gaccctgtca acgagaacct gatgcaactc ctcatcatga
     1681 tagacgccgc aaaaagggcc tctgccaaga ggatcgtggc cgtcatcccg tggtacgcct
     1741 actcgcgcca ggaccgcaag accaagccgc gcgagcccat aaccgcgcgc ctgatagcga
     1801 acatgatcca ggccgcaggg gctgagcggg ttatgacgat ggatctgcac tcgggccaga
     1861 tcgagggcta cttctctttc cctgtggatc acctgacggc gatgcacacg ttcgtcgact
     1921 atttcgtgga ccagggcttc cgggaggccg aggacgccgt cgtggttgcc cctgacacgg
     1981 gcgaggtcaa ggttgccaag cagctcgccg gacacctaag gctcccctgg gccatagtga
     2041 acaagatgcg cgacaggccg ggacactctg aagtgacgca cgtcataggg aacgtggcgg
     2101 gcaagcgggt cgtcatgatc gacgacgtaa tcgccacggg tgggaccctg gtcaacgccg
     2161 cgaagcgcct tgtggaacaa ggcgcactcg aggtcagcgc cgccgccacc cacggcgagt
     2221 tctcggggga tgcctaccgg aagatcgaag actcacctct caaggaggtc gtggttacca
     2281 acacgctgcc cttgaaggag gatgagccgc gttctaagat acagacactc aatatcgcac
     2341 cgatccttgc gagcaccatc aaaaacgtct tcacggacga gtcggtgagc gaagtgttta
     2401 tgggcgagaa ccaactcttc tagaccgtta ggagcgagac gtggcagata acgtggatct
     2461 acaggcgcgg gagcgcgaca cgcggggcaa gaacgcggcc cgacgcctca gggcctcggg
     2521 gat
//
LOCUS       DATMJA010000320         3698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_753389, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000320 DATMJA010000000
VERSION     DATMJA010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753389"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..564)
                     /locus_tag="VNT12_07680"
     CDS             complement(250..564)
                     /locus_tag="VNT12_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HWI46037.1"
                     /translation="MLHGLAVPSATSTETKPGIEIERSVTQEPPYKVILHNDDYTPME
                     HVVAVLQKVIPRMSVKRAVDIMMEAHTKGKAVATKCHKELAELYQDGLNNEGLISTIE
                     PD"
     gene            complement(656..1831)
                     /locus_tag="VNT12_07685"
     CDS             complement(656..1831)
                     /locus_tag="VNT12_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HWI46038.1"
                     /translation="MAAHTLNGQAAIVHDLALEDYGFGGDHPFNPLRVRLALELCEAL
                     GLLEDYPFVSPGPATDEDLTAVHSLTYVRMVQKAGRGAGDLSDLMDYGLGTPDNPIFP
                     HMHEACARVVGGVLAACRLVTTGEADHAICISGGLHHAMRSRASGFCVYNDAGVAISR
                     LKEEYPGIRIAYVDTDAHHGDGVQWMFYSDPDVLTVSMHESGRYLFPGSGGVDEKGRN
                     GGVGYSVNVPLEPFTDDESWISCFEGVVPAALRAFDPDLILSQNGCDGHALDPLTHLS
                     ATTRFYEYVPHRIHDLAHELCGGRWVATGGGGYDIWRVVPRAWTSLWATVSHQELPEK
                     VPEPWLSKWSGKSPVGLPPLMRDNPEDYPQGPRASKIAERNLRTVEDVVENVLSRIQ"
     gene            complement(1813..2442)
                     /locus_tag="VNT12_07690"
     CDS             complement(1813..2442)
                     /locus_tag="VNT12_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HWI46039.1"
                     /translation="MTSEIVTLAPDETVGTALALCRERRIRHLPVLTEGRLVGIVSDR
                     DLRSATPAFGDQERAAALQEILVEDVMTSDVVSVLPDDPIEQAANTMRERRIGSLPVV
                     ESGELVGIITSSDVMSALVYLVGANEPGSRMEVSVPDRPGSLAGAAGVFGMCGINIVS
                     AAMGPIRGPREEGAVRERVVVFRVDTIDTTEVVRYLEEAGYSVLWPPTP"
     gene            2540..2914
                     /locus_tag="VNT12_07695"
     CDS             2540..2914
                     /locus_tag="VNT12_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46040.1"
                     /translation="MPEYLNQLLADSATALVHENFTGVSAPWWWERRLGGGIEVCQEF
                     DPEGVSHEISAKTGREVSEVRRAIVEELGLEDAEPVVLTFEIAGETETRRVAQMLGER
                     SSEPEGLAAGLYRRIEKIVRNG"
     gene            complement(2918..3247)
                     /locus_tag="VNT12_07700"
     CDS             complement(2918..3247)
                     /locus_tag="VNT12_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI46041.1"
                     /translation="METSSYENITPRALKSRLDAGERPVLLDVRELWEFELARIEGSE
                     LIPMSQLEGRFTELDPDAETVVICHHGNRSSYVAMALQRAGFESVLNLEGGLDAYSSV
                     DESVPRY"
     gene            complement(3252..3542)
                     /locus_tag="VNT12_07705"
     CDS             complement(3252..3542)
                     /locus_tag="VNT12_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HWI46042.1"
                     /translation="MVDHLVFFAVREEASEEEVEDLVSSIRALRDEVPSTVDLSVGED
                     FSGRSGGYTHALVARFEDTAGLREYMEHPAHLAVVEKLDATTTGRIVADYEF"
BASE COUNT          677 a         1131 c         1165 g          725 t
ORIGIN      
        1 cccagcaact ccgctatctc tggtgggata cgacacggta cccggttggc gaagtcctgc
       61 agttcttcat cggagaggcc aggaaaaagc tccaacgtta cgacgttgcc atcttcgtcg
      121 atgagcgtgc ctgcctgtgc ccggcggatg atttccagag gagtcataac tggtttcttg
      181 gccaaacgcc tttgcacgat catacaggcg cactacgcgc ttcccaacgc ctcttttggt
      241 gacgtgtacc tagtcgggtt ctatagtgga aattaggccc tcgttgttga gcccgtcctg
      301 gtacagctcg gctagctctt tgtggcactt ggttgcgacg gctttgccct tggtgtgggc
      361 ctccatcatg atgtctacgg ccctcttcac ggacatgcgc gggatcactt tttgcaacac
      421 ggcgacgacg tgttccatcg gcgtgtagtc gtcgttgtgc agaattacct tgtaaggggg
      481 ctcctgggta acgctgcgct ctatctctat ccccggctta gtctcggtcg aagtggcgga
      541 agggactgct aggccatgca acatcggtat gctttgcact gtcatctccg ggtcgccgaa
      601 ctggtcacct agggcaaaat accgcgacga ggcccgatgc gcaaccctgt ttgtcctact
      661 gaatcctgga caatacgttc tctacaacgt cctcgacggt gcgaaggttg cgttccgcta
      721 tcttcgaggc acgcggtccc tgcggataat cctcaggatt gtcgcgcatg agaggcggta
      781 gcccgacagg gctctttccg ctccatttcg agagccaggg ttcaggcact ttctcgggga
      841 gttcctggtg ggagacggtg gcccagagcg aggtccaggc gcgagggacg acgcgccaga
      901 tgtcgtagcc tccgccgccg gtcgcgaccc agcggcctcc gcagagctcg tgggccaggt
      961 cgtgtatccg atggggcaca tactcgtaga agcgggtcgt tgcggagagg tgcgtaaggg
     1021 ggtcgagggc atggccgtcg cagccgttct gggagaggat caggtcaggg tcgaaggcgc
     1081 gaagggccgc ggggaccacg ccctcgaagc aggagatcca ggactcatca tcggtaaatg
     1141 gttcgagcgg cacgttgacg gagtagccga cgccgccgtt gcggcccttc tcatcgactc
     1201 cgccgctacc tgggaagagg tagcgtcctg actcgtgcat cgagacggtc aggacgtcgg
     1261 ggtcgctgta gaacatccac tgcacaccgt cgccgtggtg ggcgtcggtg tcgacgtagg
     1321 cgatacggat gccgggatat tcctctttga ggcgtgagat cgcgactccg gcgtcgttgt
     1381 atacgcagaa gccgctggcc ctggatctca tagcgtggtg cagcccgcca gagatgcata
     1441 tggcgtggtc tgcctcacct gtagtcacga gccggcatgc ggcgagcacg ccccctacca
     1501 cgcgggcgca agcttcgtgc atatggggga agatggggtt gtccggggtc ccgaggccgt
     1561 agtccatcag gtcggagagg tctcccgctc cccgtccggc cttctggacc atacgcacgt
     1621 aggtgaggct gtggacggcc gtcaggtcct cgtcggtcgc cggtccaggg gagacgaagg
     1681 ggtagtcttc cagcaacccc agggcttcgc agagctcgag tgcgagccgc acgcgcaggg
     1741 ggttgaaagg atggtcaccg ccgaagccgt agtcctccag ggccaggtcg tggacgatgg
     1801 cggcttggcc gttcaaggtg tgggcggcca caggacggag tagcctgcct cctccaggta
     1861 tcggaccacc tcggtggtgt cgatggtgtc taccctgaaa acgacgacgc gttccctgac
     1921 cgctccttct tccctgggcc cccttatcgg ccccatcgcc gcgctgacga tgtttatgcc
     1981 acacatcccg aagacgcctg ccgcgccggc cagggaccct ggcctgtcag ggaccgagac
     2041 ctccatgcgg ctccctggct cgttggcgcc aaccaggtag accagtgcac tcattacgtc
     2101 agaggaggtg atgatcccga caagttcccc gctctcgacg acggggaggc tgccgatcct
     2161 gcgctctcgc atcgtgttcg ccgcctgctc tataggatcg tcggggagga cactcaccac
     2221 gtcgctggtc atcacgtcct ccaccaggat ctcctgcagc gcggcggccc gctcctggtc
     2281 cccgaacgcg ggggtcgccg agcgcaggtc cctgtccgag acgatcccga cgagcctccc
     2341 ttccgtaagg acaggtaggt gtctgatgcg cctctccctg cacagggcga gcgccgtccc
     2401 caccgtctca tccggcgcga gcgtcacgat ctcactggtc atcgagtctc ttacaagaag
     2461 cattggataa ctccttccct caccgcattg tatagtagcg gatcgccgcg gttaagcgtg
     2521 cggctggtat aaatcaccca tgcccgaata tctcaaccaa cttctcgccg acagcgcgac
     2581 ggcgctggtg catgagaact ttacaggcgt cagcgccccg tggtggtggg agcggcgtct
     2641 gggaggaggc atcgaggtct gccaggagtt cgaccctgaa ggcgtgagtc atgagatctc
     2701 agcgaagaca ggccgcgagg tctccgaagt ccgccgggct attgtcgagg aactcggcct
     2761 cgaagacgcc gagcccgtgg tcctcacttt cgagatagcg ggcgagaccg agacccgccg
     2821 cgtcgcccag atgctaggag aacgttcctc agaaccggag ggcctcgccg cagggctcta
     2881 ccgacggatc gagaagatcg tcaggaacgg gtaggagtca atacctcggg acagactcgt
     2941 ctacggagga gtaggcgtcg agaccgcctt ccagattgag gacgctctcg aatccggccc
     3001 gctggagggc catggcgacg taagagctgc ggttgccgtg gtggcagatc acgacggtct
     3061 cggcatcagg gtctagctcc gtaaagcgcc cctccagttg ggacatgggg atgagctctg
     3121 agccctctat gcgggcgagc tcgaactccc agagctcgcg cacatcgagc agcacgggac
     3181 gctctccagc atcgagcctg ctcttcagag cgcgcggcgt tatgttttcg taggaggagg
     3241 tctccatgtc gctagaactc gtagtctgcg acgatgcggc ctgtcgtggt ggcgtcgagc
     3301 ttctcgacta cagccaggtg cgctggatgc tccatgtact cccgaagacc ggcagtgtcc
     3361 tcgaacctgg cgacgagagc gtgggtatat cctccggagc gccccgagaa gtcttcaccc
     3421 acggagaggt caaccgtgct aggcacctca tctctcaacg cacggataga cgagacgaga
     3481 tcttcgacct cttcttccga ggcctcttcc cgaacggcga aaaacacgag atggtccacc
     3541 ataactctct cccctcgatc ccgaacatgc cctgcgaagt ctagcaggaa ggccgcaaaa
     3601 ggatgcgggg agctggctca acaatcttat ggtcgccgat ggaaggggct catctggtcc
     3661 caagatctgt tcccggagaa tccctgacac ctgaagcc
//
LOCUS       DATMJA010000321         6183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_753464, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000321 DATMJA010000000
VERSION     DATMJA010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753464"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VNT12_07710"
     CDS             <1..490
                     /locus_tag="VNT12_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWI46043.1"
                     /translation="GRARERAADLDGDAVVVAEPVVENSVGAGTQLCSTFEELGEVAR
                     RGGVRVCVDTAHAFAVGYDLSSENGGRGVAEELVNAVGEAVALIHLNDAKNELGSHRD
                     GHSKVGEGCIPVDVWPGFFDGLPGVPVVMETPYGSPEADSEEVLLVKKLAGGLRKAPI
                     GR"
     gene            501..800
                     /locus_tag="VNT12_07715"
     CDS             501..800
                     /locus_tag="VNT12_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI46044.1"
                     /translation="MKTLEISPERHAGDLLSTIGETLRAERNEQGLTLKQVAEGAHVS
                     VSYLAEIERGEKDPSSRVLESIASGLDIEVSELLVRIASALEPAPVVPVTDSLAA"
     gene            819..1307
                     /locus_tag="VNT12_07720"
     CDS             819..1307
                     /locus_tag="VNT12_07720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWI46045.1"
                     /translation="MRFTLKPITPSDARAISRWRYEEPYSIYDGDPTSVDALLQPRFS
                     YHSVYDERGDLAGYFCFGEDAQVAAGKRLGVYEVEPALDVGLGMRPDLTGRGLGAEFL
                     RAGLRFAREVYSPPAFRLTVAAFNHRAIRVYERAGFEIVETFGAPTLGAEREWLLMRC
                     GA"
     gene            complement(1324..2355)
                     /locus_tag="VNT12_07725"
     CDS             complement(1324..2355)
                     /locus_tag="VNT12_07725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWI46046.1"
                     /translation="MLTSVGVMQYLLSSLATMVFIGVLALLAQWSRKSRRAEISLLIV
                     LVFLSLLLFATGALLGGVWVSGQMPPEVYPQQLLAVTAVPVAMSGLIGLALCVPTLLK
                     IVRGRPNGEFWTDPPIFLALWLFATVLLANNLVGIVGFEQLNQVDAFSLGTGGRIPPV
                     AILASQLPFVVIALLGVGAGIRRGPRETLVRLGYGPISLRHVGIVVLFIVGAFAISVA
                     ADALFSQLQPDVYRRVGEISKALFDPKGLSVVAAVLFALLIGVGAGLGEETLFRGAVQ
                     PVLGIPVTSVLFASMHVQYGPSLLLGYIFVLSIGLGLLRRYVNTTASFLAHAGYNTLG
                     ILAAYFFGM"
     gene            complement(2500..2790)
                     /locus_tag="VNT12_07730"
     CDS             complement(2500..2790)
                     /locus_tag="VNT12_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46047.1"
                     /translation="MHTDPPVELRLPLEEAEALHALLERLLESGEQDPRLEHSYRLLG
                     WRMLAAKGGKGLTGRIADLAREADSLEEYEAAREKQLGPVLHGLERGENRDP"
     gene            complement(2792..3139)
                     /locus_tag="VNT12_07735"
     CDS             complement(2792..3139)
                     /locus_tag="VNT12_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46048.1"
                     /translation="MGNRRVALKPHASKIRQWVEEGRGDTWIAQELNTTPSSVQSFRS
                     RNSIYRRDPVRRGQLSEHPAVLEEAEGGIVLKTDATDSEVFDREWRHYLRGSPEDLQV
                     VITQDRIYLEKVR"
     gene            complement(3439..4440)
                     /locus_tag="VNT12_07740"
     CDS             complement(3439..4440)
                     /locus_tag="VNT12_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI46049.1"
                     /translation="MSKNQRSCVVVGAGVSGLLAAKSLNEKGWTVTVLDKSRGVGGRM
                     ATRRVGEGNFDLGAQFFTVRGERFAGLVEDWLGAGVATEWTRGFADSEGRPNEDDYPR
                     YRGAEGMTSIPKYISCGLDVRTGERVVRVDDNDNGWEVVCESGSRVAGNALVLTAPVP
                     QTLDLASSGSYSLPDGARRHLQAVSYDPCLALMVLLDDSTGVPEPGGMQIKGEPLDWI
                     GDNRRKGISEAPAVTVHAGPEWSRSHFEDDEMEITESMISRAGDHLGTDLAPAVVETS
                     LARWRYSWVTNPHPNPCLVASDDPPLVFCGDAFGQPKVEGAALSGLAAADHLLGRNA"
     gene            complement(4437..5372)
                     /locus_tag="VNT12_07745"
     CDS             complement(4437..5372)
                     /locus_tag="VNT12_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWI46050.1"
                     /translation="MKVFYSDHFVLPLPEGHRFPMVKYSMLRERVTRDGICGPGELLT
                     PRAVTDQEILRAHAPDYLKRVASGTLTEREVRRIGFPWSERMVERSRRASGGTLGACL
                     AALDDGFAANLAGGTHHAFSDRGEGFCVFNDSAIAARAVQAAGLVERVVVIDTDVHQG
                     NGTAAILRGDPTVFTYSIHGAKNFPFHKEESDLDAPLPDGAGDTEFLATLERGLEAAL
                     HAVDADLAIYLAGADPFERDRLGRLSATKSGLADRDRIVLETCRERGIPVAVTMAGGY
                     AYEVADTVDVHFQSIRRAAELLESTADRSKETHLT"
     gene            5490..>6183
                     /locus_tag="VNT12_07750"
     CDS             5490..>6183
                     /locus_tag="VNT12_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWI46051.1"
                     /translation="MDDSAQQRLFAETPGSIEPPEEKLYLGTSGWSYADWEGTVYPPG
                     TTPAGRLAEYVKHFTTVEIDSTFYGTPRVTTVERWRNAAPRGFLFAAKFPQEVTHEGN
                     LVNIRSEAEGFVHTMQTLEDKLGPLLLQLPPDFSVEGMGVLEDFLESLPEGPRYAVEV
                     RHRSWIGSDLPELLRECGAALTLVDYPRMPRMQEATTEFVYIRWLGNRREFPEGHTHL
                     KKDRDDDLLWWSD"
BASE COUNT         1175 a         1908 c         1961 g         1139 t
ORIGIN      
        1 ggggcgtgct agggagcggg ccgctgatct cgacggagac gcagtagttg tggccgaacc
       61 tgtagtcgag aactccgtcg gggccggtac gcagctctgc tcgacgttcg aggagctcgg
      121 ggaggtcgcg cgacgcggcg gggtgagggt ctgcgtggac acggcccacg ctttcgctgt
      181 tggctacgac ctgtcgtcgg agaacggagg gcggggggtc gcggaggagc tcgttaacgc
      241 agtgggagaa gcggtcgcgc tgattcacct caacgacgcg aagaacgaac tcgggagcca
      301 ccgcgacggg catagcaagg tgggggaggg gtgcataccc gtcgatgtct ggccagggtt
      361 tttcgacggt ctgccaggag ttcccgtggt catggagacg ccttacggga gccctgaggc
      421 tgactccgaa gaggtgcttc tggtcaagaa gttggcgggt gggttgcgca aagcgccgat
      481 tggaagatag aatctagggc gtgaagactt tggagataag cccggagcgg catgcaggag
      541 accttctctc gaccatcggc gagaccctgc gggcggaacg caacgagcag gggctgacgt
      601 taaaacaggt cgctgaaggc gcgcacgtct cggtctcata cctcgccgag atagaacgtg
      661 gcgagaagga cccctcctcg agggtacttg agagcatcgc cagtggattg gacatcgagg
      721 tcagcgagct gctcgtccgg atcgcctcgg cgctcgagcc cgcgccggtc gtccccgtca
      781 ccgactctct ggcggcctag actcccaaga agccgacgtt gcgcttcacc ttgaagccga
      841 tcacgcccag cgacgcacga gctatctcgc gctggcgcta cgaagaaccc tactccattt
      901 acgacggaga tcccacttct gtggatgcgc tcctgcaacc gcgcttctcc taccactctg
      961 tctacgacga acgcggagat ctggcgggct acttctgctt cggcgaggac gcgcaggtcg
     1021 cggccggaaa gcggctcggg gtgtacgagg tcgagcccgc gctcgatgtc gggctcggga
     1081 tgaggccgga tctcacagga cggggccttg gcgcggagtt tctgcgcgcc ggcctccggt
     1141 tcgcacggga ggtctattca cctccggctt tccgcctgac cgtggccgcc ttcaaccacc
     1201 gcgctatccg cgtctatgaa agggccggat tcgagatcgt agagactttc ggcgctccaa
     1261 cgctgggcgc cgaaagggaa tggctgctga tgagatgcgg ggcgtaggat cagccgccgt
     1321 tctctacatg ccgaagaagt aggcggccag gatgccgagt gtgttgtagc ctgcatgcgc
     1381 caggaagctc gccgtagtgt tgacgtagcg gcggaggagt ccgaggccga tggagagcac
     1441 gaagatgtag ccgagcagca gcgatggacc gtactgcacg tgcatcgagg caaaaaggac
     1501 agaagttacc ggtatcccca ggaccggctg cacggcgccg cggaagagcg tctcctcacc
     1561 gagtcctgca cccaccccga tcagcaaggc gaagagcacc gccgcgacca cgctcaaacc
     1621 tttcgggtcg aagagcgcct tggagatctc acctaccctg cgataaacgt caggctgcaa
     1681 ctgcgagaac agggcgtcgg cggcaaccga gattgcgaac gcacctacga taaacagcac
     1741 gacaatacct acgtgcctga gggagatggg accgtagccg aggcggacga gcgtctcgcg
     1801 cggtccgcgg cggatgccgg cgccgacccc cagaagggcg atgacgacga agggcaactg
     1861 gctcgccagg atggcgactg gtgggatcct accaccggtt cccaaagaga aggcgtcgac
     1921 ctgattcaac tgctcgaatc cgactatgcc taccagatta ttggcaagca acacagtggc
     1981 gaacagccag agagccagaa agatcggtgg gtctgtccag aactccccgt tcggcctgcc
     2041 gcgaacgatc ttcagcagcg tggggacgca cagcgccagc ccgatcaaac ccgacatcgc
     2101 aacaggtact gccgtgaccg ccaacagctg ttgcggatag acttcagggg gcatctggcc
     2161 cgagacccac actccaccaa gcaatgcccc cgtggcgaag agcaggagcg agaggaacac
     2221 gagcacgatc aagagcgata tctcggccct cctgctcttt ctcgaccatt gagcgaggag
     2281 cgcgagtacc ccgatgaaga ccattgtcgc gagcgaactg aggagatact gcatgacgcc
     2341 aacagaggtt aacacatgca agataccgga ccgcgcatga tccgcggccc gggctgctct
     2401 tctgggccag gtaaggggaa agagcccagt gcccggaaaa atagagcacc gaacttacaa
     2461 taccgctacc ccaaggcaac agtttgctaa caagttaggt catggatccc ggttctcgcc
     2521 gcgctccagg ccatgtagca cgggacccag ctgtttttcg cgcgccgctt cgtactcttc
     2581 gaggctgtcc gcttcccgcg ccaggtctgc tatacgtccc gtgagacctt tgccgccctt
     2641 tgcggccagc atacgccagc ccaggagcct gtaggagtgt tcgaggcgcg ggtcttgttc
     2701 cccgctttcg agaaggcgct ccagaagcgc gtgcagtgct tctgcctcct ccagagggag
     2761 ccgcaactcg acggggggat ctgtgtgcaa gctagcggac tttctccagg tagatcctgt
     2821 cctgcgtgat caccacttgc aggtcttccg gcgagccccg caggtagtgg cgccactcgc
     2881 ggtcgaagac ctccgagtct gtggcgtcgg tcttcaggac tatgccacct tctgcttcct
     2941 cgagcaccgc aggatgctcc gagagctggc cgcgccggac ggggtccctg cggtatatcg
     3001 agtttcgcga ccggaagctc tgtacgctag acggcgtggt gttcaactcc tgcgcgatcc
     3061 acgtgtcgcc ccgaccctct tcgacccact gcctgatctt ggacgcgtgc ggctttagcg
     3121 ccacccttct gtttcccaag gcccctttct ccttcacact gtgttgtttt ctaggtcata
     3181 tgtaccactt tttgttaccc gacgctagat gcatccgtac acttctcgct cacagtgagt
     3241 tgcagaaggt caatccttcc gcaactcgcg ctgtcagcac tcagcaatca gcactcagcc
     3301 ttttggttgc caaccgctga tggccaactg ctgatagctc gtcgtgcata agggttggcc
     3361 cctatgcacg gcgctgtagt agccgctggg acgcggggtc gccgcgacgt gaacgaggaa
     3421 caacaaggac acggggtatc aggcattgcg gccgaggaga tggtctgcgg cggctaggcc
     3481 tgagagggct gcgccctcga ccttgggctg cccgaaggca tcgccgcaga acaccagtgg
     3541 cgggtcatcg gaggccacga ggcacggatt ggggtggggg ttcgtaaccc agctgtagcg
     3601 ccacctggca agggaagtct cgacgacagc gggtgcgagg tcggtgccga gatggtcgcc
     3661 agcgcgcgag atcatagact ctgtaatctc catctcgtcg tcctcgaagt gcgagcggct
     3721 ccattctggt ccggcgtgga ccgtgacggc cggggcttcg gagatgccct tgcgtctgtt
     3781 gtcgccgatc cagtcaagcg gttcgccctt gatctgcatc cccccgggct ccggaacacc
     3841 ggtcgaatcg tcgagcaaga ccatcagggc gaggcatggg tcgtaggaga ccgcttggag
     3901 gtggcgcctg gcgccatccg gcagactgta gcttcctgac gaggcaagat ccaatgtctg
     3961 aggaacagga gccgtgagca cgagggcatt ccctgctacc ctcgatccgg actcgcacac
     4021 gacctcccag ccattgtcgt tgtcatcaac ccttacaacc ctctctccgg tgcgtacgtc
     4081 gaggccgcag gagatgtatt tcgggatgga ggtcatgccc tcagcccccc tgtagcgagg
     4141 ataatcgtct tcgttcggtc gaccctctga gtcggcgaag ccgcgcgtcc attcggtggc
     4201 gacgcctgcc ccgagccagt cttcgacaag gccggcgaag cgttcgcccc tgacggtgaa
     4261 gaactgggcg ccgagatcga agttaccttc cccgacccgc ctggtagcca tcctgccgcc
     4321 gacgccacgg ctcttgtcca gaaccgtaac ggtccaacct ttttcattca gtgacttggc
     4381 cgcgagcaga ccggagacgc cggcccctac gacgacgcag gatctctgat ttttgctcac
     4441 gtcaggtgtg tctctttcga tctatccgct gtactctcca gcagctcggc cgccctacgg
     4501 atgctctgga agtgtacgtc caccgtgtcc gctacctcgt aggcatagcc tcccgccatg
     4561 gtaaccgcaa cagggattcc gcgttccctg caggtctcta ggacgatccg gtctcggtcg
     4621 gcgaggcctg acttcgttgc ggagagccgg ccgaggcggt ctctctcgaa agggtcggcg
     4681 cctgcgaggt agatggcgag gtctgcgtcc acggcgtgga gcgccgcttc caggcctctt
     4741 tcgagcgtcg ccaggaactc cgtatcgccg gcgccgtcgg ggagtggcgc gtccaggtcg
     4801 ctctcctctt tgtggaaggg aaagttcttc gcgccatgga tcgagtacgt aaacaccgtg
     4861 gggtctcccc gcaggatcgc cgccgtcccg ttcccctggt ggacatccgt gtcgatgacg
     4921 actacgcgct ccacgagacc tgcagcctgc acggcgcggg cggcgatggc cgagtcgttg
     4981 aagacgcaga atccctcgcc cctgtcggag aacgcgtggt gcgtcccacc agcgaggttg
     5041 gctgcgaagc cgtcgtcgag ggcggcgagg cacgccccga gcgtgccgcc cgaggcgcgg
     5101 cgggagcgtt ctaccatcct ctccgaccac gggaaaccga tgcgccgcac ctccctttcc
     5161 gtgagcgttc cggaggcgac gcgtttcagg tagtcggggg cgtgggcgcg caggatctcc
     5221 tgatccgtta ccgcgcgtgg cgtcaaaagc tctcccgggc cacagatccc atcgcgcgta
     5281 accctctcgc gcagcatcga gtacttgacc atcgggaacc ggtggccctc gggcaggggt
     5341 agtacgaagt ggtcgctgta gaagaccttc attgggtctg gcgtctctcg tccatggcac
     5401 caatgtagta catgcggtgg gagtgaacag gaagagtttt catacaaacc gcagggactc
     5461 gtatggtagc atctcaactt cgagggcgga tggacgattc tgcacaacag cggcttttcg
     5521 cggagactcc cggcagcatc gagccgccgg aggagaagct gtacctgggc acctcaggct
     5581 ggtcatacgc ggactgggag ggtactgtct accctccggg gactactccc gccgggaggc
     5641 tcgccgaata tgtaaagcac ttcaccacgg tagagattga ctcaaccttc tacggcaccc
     5701 ccagagtaac gaccgtagag cggtggcgga atgccgctcc ccgtgggttt ctcttcgctg
     5761 ctaagtttcc tcaagaggtg acgcacgagg gaaacctcgt caatatccgt agcgaggccg
     5821 aaggcttcgt ccacaccatg cagaccctcg aagacaagct cggtcccctg ctgctccagc
     5881 ttccgccgga cttcagtgtc gagggcatgg gtgtcctcga agattttcta gaaagcctgc
     5941 ccgagggacc acggtatgcc gtggaggtac gccacaggag ttggattggc tcggacctcc
     6001 ccgagttgtt gcgagagtgt ggtgcggcac tcaccctggt ggactacccg aggatgcccc
     6061 gcatgcagga ggcgacaaca gagttcgtct acatccgctg gctcggcaac cggcgcgagt
     6121 tccctgaggg acacacccat ctcaagaagg accgagacga tgacctattg tggtggtcgg
     6181 atc
//
LOCUS       DATMJA010000322         4079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_279600, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000322 DATMJA010000000
VERSION     DATMJA010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279600"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VNT12_07755"
     CDS             <1..473
                     /locus_tag="VNT12_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46052.1"
                     /translation="ASEAPFPGRDLIIFLTFCVILATLVVQGLSLPVLIRALGLEDDR
                     IGDKEETHGRIRIAEAALERLDELADEAWVREDTAERIRGLYSYRRDRFASRFEGDPD
                     GVEERSAAYQRLMVELLGAQRLRLITMRDEGSIGDEVMHRIERDLDLEESRLEL"
     gene            complement(568..1545)
                     /locus_tag="VNT12_07760"
     CDS             complement(568..1545)
                     /locus_tag="VNT12_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003772115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HWI46053.1"
                     /translation="MIAARLQRGDEVRIVSPAVSLGFIPEDQQLTARERLEGLGLRCS
                     FSPNAEVMDRFDSSPVEARVSGLHDAFADPGVKGILTTLGGYNSNGLLGYLDYDLIRA
                     NPKIFCGFSDITALATAINARTGLVTYSGPHFTTFGMKRGIDYTKEYFERCLMREEPF
                     EVLPADYWSDDLWYQDQENRDFVPNSGYEVMYEGEAEGKLLGGNLGTLCLLFGTPYMP
                     NLQGSILLLEDDEEVNAVHFDRELQSLIHQPGFEGVRGIMFGKFQRASNMDQNTLMAI
                     VESKHELDGMPVIAGASFGHTTPQFTFPIGGYGSLHAGEGTARLYIDVH"
     gene            complement(1558..2580)
                     /locus_tag="VNT12_07765"
     CDS             complement(1558..2580)
                     /locus_tag="VNT12_07765"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWI46054.1"
                     /translation="MRSGTHTFLQTTRVHFGVDALENLEEEARSYDRAFVITGRSLNE
                     KTGLISRIEAMLGGRHAGTFAGIGQHTPGGAVEKAAVEAELAAANLLVSVGGGSVIDG
                     TKAVARELGYPQQVAIPTTLSGAEWANRAGVTDERSGRKRGFADPKTVPRVVVLDPEA
                     TVFTPEKLWLSTGIRALDHAVEGFLYGDDHPITDLTGLEGARRLMESLPLSRSTPEDL
                     EVRQELQLAAWLSYFGPANTPMGLSHELGRRIGASYEVPHGITSCIILAPSLQVMKER
                     VQDDRWNMLSRTLDGEPPERVSSLVEELGLPGRLREVGVPEEDLEDIAGEYGDGEALA
                     ILRAAY"
     gene            complement(2668..3528)
                     /locus_tag="VNT12_07770"
     CDS             complement(2668..3528)
                     /locus_tag="VNT12_07770"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HWI46055.1"
                     /translation="MILVSGATGQQGGAVARSLLGRGIGVRALTRDPEKPEAKELVDL
                     GAEVASGDLEDRSSIERVLDGVHGVFSVQQSWETGVEGEIRQGVLLADAAKAAGVDHY
                     VYSSVGSAHRETGIPHFESKWEVEEHVRGSGVPYTVLRPVFFMQNWEFLREPILGGTL
                     PQPLDPDKSFQMVDANDIGVFAAMAFENPGEWIGREVDLAGDEMTMPQIADTFSRVIG
                     RQVDYFQVPWYQFEEQMGEEYAVMYRWFDEHGYEADIAALKEEHPGLVAFEQYLRANG
                     WENAGSSPEG"
     gene            3681..>4079
                     /locus_tag="VNT12_07775"
     CDS             3681..>4079
                     /locus_tag="VNT12_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI46056.1"
                     /translation="MGRGRIQGAGWSLDHLGRPSGCEAERSDAARNRRVILRTARTLF
                     EERGVTCVTMEEIARASGVGKGTLYRRFPHKGFLCQALLDEPTRHLQEQTLEILREPG
                     TPAVEKLDAFLDRLVAFTAENLDLLYGGNEP"
BASE COUNT          726 a         1318 c         1240 g          795 t
ORIGIN      
        1 cggcgtctga ggcgccgttc cctggccgtg acctcatcat ctttctgacg ttttgtgtga
       61 tcctggcgac gctcgtagtc cagggtctct ccctaccggt cctgatccgg gccctcggcc
      121 tggaggacga ccgcatcggg gataaggaag agacccacgg ccgcatcagg atcgctgaag
      181 ccgccctgga gcgcctggac gagcttgcgg acgaggcctg ggtgcgcgag gacacggctg
      241 agcgcattcg cggcctctac agctaccgcc gtgaccgctt cgcctcccgc ttcgagggcg
      301 atcctgacgg cgtcgaagaa cgctctgccg cctaccagcg cctcatggtc gagctgctcg
      361 gggcgcagcg cctgaggctc attacaatgc gcgacgaggg aagcatcggg gacgaggtca
      421 tgcaccgcat cgagcgcgac ctcgacctgg aagagtcgcg cctggagttg tagtgcactt
      481 tgcaagcagt tcagctttct cgtgcgcgag tcctggctga cttgctgacc agcgcggttc
      541 caagacgttc tcttcctaaa actgctttca gtggacgtct atgtagaggc gagcggttcc
      601 ctctccggcg tgcagactgc cgtaaccgcc gatcgggaac gtgaactgcg gcgtggtgtg
      661 gccgaaactc gcgccggcga tgacaggcat accgtccaac tcatgcttgc tctcgacgat
      721 ggccatcagg gtattctggt ccatgttgga agccctctgg aacttcccga acattatgcc
      781 ccgcacgccc tcgaaaccag gttggtgaat gagagattgc aactcccggt cgaagtggac
      841 ggcattgacc tcctcgtcgt cctcgaggag gaggatggaa ccctgcaggt tcggcatgta
      901 cggggtgccg aagagcaggc acagggttcc caggttcccc ccgagcagct tgccctccgc
      961 ctcgccttcg tacatgacct cgtagccgga gttgggtacg aagtcacggt tctcctggtc
     1021 ctggtaccag agatcatcgc tccagtagtc agcgggaagg acttcgaagg gctcctcgcg
     1081 catgaggcac cgctcgaagt actccttcgt gtaatcgatg ccgcgtttca tgccgaaggt
     1141 cgtgaaatgc ggccctgagt aggtgacgag gccggtcctg gcgtttattg ctgttgcaag
     1201 agccgtaatg tccgagaaac cacagaagat cttggggttc gcgcggatca gatcgtagtc
     1261 cagatacccg agtagcccgt tgctgttgta gccacccagg gtggtgagta tgcctttgac
     1321 accgggatcc gcgaaggcat cgtgcagacc ggagacacgc gcctctacag gggaggaatc
     1381 gaagcgatcc atcacctcgg cgtttgggga gaacgagcaa cggaggccta gaccttcgag
     1441 ccgctccctg gctgtcagct gctgatcttc tggtatgaat ccgaggctga cggccggtga
     1501 gacgatcctt acctcatcgc ccctctggag tctcgcggcg atcattgccc gcgcatccta
     1561 gtacgcggcc ctcaggatcg ccagcgcttc cccgtcgcca tactccccgg cgatatcttc
     1621 caggtcctcc tcaggcaccc cgacttcgcg aagccgaccg ggcaatccga gctcctcgac
     1681 cagagatgac acgcgctctg gcggttctcc atctagagtc cgagagagca tgttccaccg
     1741 gtcgtcctgt acgcgctctt tcatgacctg caggcttggt gcgaggatga tacaggaggt
     1801 tattccgtgc gggacttcgt agctggcccc gatcctgcgc cccagctcgt gcgagagacc
     1861 catcggcgtg ttcgccggtc cgaaatacga gagccaggcc gcgagctgta gctcctggcg
     1921 cacctccagg tcttcgggtg tgcttctgga aagcggcaga gactccatca ggcgccgcgc
     1981 cccttcgaga ccggttaggt ctgtgatggg atggtcgtct ccgtagagaa agccttcgac
     2041 cgcgtgatcc agggccctta tgccggtgga gagccagagc ttctctggcg tgaagaccgt
     2101 agcctcagga tccagtacga cgaccctcgg aacggtcttc gggtcggcga agcctctctt
     2161 cctaccggat ctctcgtccg taacccctgc cctgttggcc cactccgcgc cggagagggt
     2221 ggtcggaatc gccacctgct gaggataacc gagctcccgc gccaccgcct tggtgccgtc
     2281 tatgacgctt cccccaccga cgctgaccag gagattcgcc gccgccaatt ccgcctcgac
     2341 ggcggctttc tcgaccgccc caccaggcgt atgctgcccg ataccggcga aggtccccgc
     2401 gtgcctgccc ccgagcatcg cctcgatgcg gctgatgaga ccggtcttct cgttcaggct
     2461 gcgcccggtg atgacgaagg cccggtcata cgaccgggcc tcttcttcca agttctccag
     2521 agcatccacg ccgaaatgaa cgcgcgtggt ctgaaggaag gtgtgggtgc cgcttctcac
     2581 gctcctatcg tacagctatg cgcggctcca ggcgaccgcg acgaagcgct actcatttac
     2641 ccggatgagc tggcacctgt tctcagatca accttccggg gaagatccgg cgttctccca
     2701 gccgttggcg cggaggtact gctcgaaggc gacgagcccc gggtgctcct ctttcagagc
     2761 ggcgatatcg gcctcgtagc cgtgttcgtc gaaccaccgg tacatgacgg cgtactcctc
     2821 gcccatctgc tcctcgaact ggtaccaggg cacctggaag tagtcgacct ggcgtccgat
     2881 cacgcgcgaa aacgtatcgg ctatctgggg catggtcatc tcatcccccg cgaggtctac
     2941 ctcacgtccg atccactcgc ccgggttctc aaaggccata gcggcgaaga cgccgatgtc
     3001 gttggcgtct accatctgga aagacttgtc ggggtcgagc ggctgaggga gcgtgccgcc
     3061 gaggatcggc tctctcagga attcccagtt ctgcataaag aagaccgggc gcaagaccgt
     3121 gtaaggtacg ccactgccgc gcacgtgctc ctcaacctcc cacttgctct cgaagtgcgg
     3181 aattccagtc tccctgtgcg cgctgccgac cgagctgtag acgtagtggt cgacgccagc
     3241 agccttcgcg gcgtcagcga gcagaacacc ctgcctgatt tcaccctcga cgcctgtctc
     3301 ccaggactgc tggacggaga acaccccgtg gacgccatcg agcacccgct cgatggacga
     3361 gcggtcctcg aggtccccgc ttgcgacctc ggcccccaga tcgacgagct ctttcgcctc
     3421 gggcttctcg ggatctcggg tgagcgcacg taccccgatc ccgcgcccta agagactccg
     3481 cgccaccgct cccccttgct ggcccgtggc cccgctgacc aggatcaaac gcccccatta
     3541 cgcccgtctt ccataattac tcctacactc tgatcgttct acgactgact acaccataaa
     3601 agcggacgtg agtccgcttt aaagtgcgag ggtagcgcgg tagactgccg gtgtcaagga
     3661 cttaatggag agtgatagtc gtggggcgtg gacggataca gggggcgggg tggagcttgg
     3721 atcacctggg gcgtccctct ggctgcgagg ctgagcgcag cgatgcggct cgcaacagac
     3781 gtgtgatcct gcgtacggcg cgaacgctct tcgaggagcg cggcgtcacg tgcgtcacga
     3841 tggaagagat cgccagggcc tcgggtgttg gaaagggtac gctctaccgg cgctttccac
     3901 acaagggttt cctgtgccag gcgcttctcg acgagcctac caggcatctc caggagcaga
     3961 cgctggagat cctgcgcgaa ccagggacgc cggccgtcga gaagctcgac gctttcctgg
     4021 accggcttgt ggcctttacc gcggagaacc tggacctgct ctacggcggc aacgaaccg
//
LOCUS       DATMJA010000323         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_516804, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000323 DATMJA010000000
VERSION     DATMJA010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516804"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VNT12_07780"
     CDS             <1..281
                     /locus_tag="VNT12_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWI46057.1"
                     /translation="EFGAPEAVLRYCIQKGSICVDGISLTIVSIGEGTFTVSILPQTR
                     ANTNLGELGAGSRVNLESDLIGKYVERLLEPRLVGRLSTFERSAADAF"
     gene            271..1473
                     /locus_tag="VNT12_07785"
     CDS             271..1473
                     /locus_tag="VNT12_07785"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWI46058.1"
                     /translation="MPFSPIEEIVEDIKQGKMVIVCDDEDRENEGDLTMAAELVTADD
                     INFMAAYGRGLICLPMAGEIVDRLDIPQMVTHNASRMGTAFTASIEAKEGITTGISAA
                     DRAHTCRVAVDEATGPEDLVMPGHVFPLRAKDGGVLQRAGQTEAAVDLARLAGFKPAG
                     VICEIMKEDGTMARVPDLERFSREHGVKLVTVAQIIEYRHAFETQVRCAVETRLPTPF
                     GKFRLRAYENDIDELTHVALVMGEPEGKDDVLVRVHSACLTGDALHSLRCDCGEQLEA
                     AMGRIAAEGEGVIAYMQQEGRGIGLLNKMRAYHLQDEGMDTVEANQKLGLAPDLRDYG
                     VGAQILKDLDLKRIRLLTNNLTKVVGLRGFGLEITERVPIEMEPNGHNERYLKTKREK
                     LNHVFEKF"
     gene            1585..2103
                     /gene="ribE"
                     /locus_tag="VNT12_07790"
     CDS             1585..2103
                     /gene="ribE"
                     /locus_tag="VNT12_07790"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWI46059.1"
                     /translation="MEGVNHIEGNLSAEGRSFGIVASRFNDFVVKALLEGAIEAIRRH
                     GGVAEAVDVVWVPGSYEIPVVARELALSGRYDALICLGAVIRGATAHFDYVAGGAAGG
                     ISNVALETGVPVIFGVITTESIEQAIERAGTKMGNKGFEAAVSAMEMADLMAKLRARA
                     SQDDTSTTLYNP"
     gene            complement(2107..2322)
                     /gene="rpmB"
                     /locus_tag="VNT12_07795"
     CDS             complement(2107..2322)
                     /gene="rpmB"
                     /locus_tag="VNT12_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWI46060.1"
                     /translation="MAKVCYSCGKKPDFGNARSHSLRATRRRRDPNLQKVRIQEGSAT
                     RREWVCTSCLKRFKVTKASVGAPSPSV"
BASE COUNT          536 a          749 c          854 g          434 t
ORIGIN      
        1 gggagttcgg ggcgcccgag gcggtgctcc gctactgcat ccagaagggc agcatctgcg
       61 tcgacggcat aagcctgacg atcgtctcga tcggggaggg cactttcaca gtctcgatac
      121 taccccagac ccgcgcgaac acgaacctgg gagagctcgg ggcggggagc agggtgaacc
      181 tcgagtccga cttgataggt aagtacgtag agcggctgct cgagccgcgc ctggtaggga
      241 gactttcaac cttcgagagg agtgccgcag atgcctttta gtcccataga ggagatcgtc
      301 gaggacatca agcagggcaa gatggtcatc gtttgtgacg acgaggaccg tgagaacgag
      361 ggcgacctga ctatggccgc cgaactcgta accgcggatg acatcaactt catggccgcc
      421 tacggtcgtg gtctgatctg cctaccgatg gcaggggaga tcgtcgaccg cctcgacata
      481 ccacagatgg tcacccacaa cgcttcgcgg atgggtacag cgttcacggc ctcgatcgag
      541 gcgaaggagg gcatcacgac gggcatatcc gccgccgaca gggcccacac ctgcagggtc
      601 gccgtggacg aggcgacagg cccggaagac ctcgtgatgc caggacacgt attccctttg
      661 cgggcgaagg acgggggcgt cctgcagcgc gccggccaga ccgaggccgc cgtcgacctc
      721 gccaggctgg ccggattcaa gcctgcgggt gtgatctgcg agatcatgaa agaggatggc
      781 acgatggccc gtgtacccga cctcgagagg ttctccaggg agcatggcgt caaactggtg
      841 acggtggccc agatcatcga gtaccgtcac gccttcgaga cacaggtcag gtgcgccgtc
      901 gagacgcgtc tgccaacccc cttcgggaag ttcaggctgc gcgcctacga gaacgacata
      961 gatgaactta cccacgtcgc gctcgtcatg ggcgagcccg agggtaagga cgatgtgctc
     1021 gtcagggtcc actcggcatg cctgaccggc gacgcattgc actccctgcg ctgcgactgc
     1081 ggcgaacaac tggaggccgc gatggggcgc atcgcggcgg aaggcgaagg cgtcatagca
     1141 tacatgcagc aggaaggtcg gggcatcggg ctcttgaaca agatgagggc ttaccatctg
     1201 caggatgagg ggatggacac ggtcgaagcg aaccagaagc tcggcctcgc ccccgacctg
     1261 agggactacg gggtaggggc ccagatcctc aaggatctcg acctcaaacg catccgtctt
     1321 cttacgaaca acctgacgaa ggtcgtcggg ctcagaggct tcgggctcga gatcaccgag
     1381 cgtgtcccga tcgagatgga gcccaacggc cacaacgagc gctacctgaa gacaaagcga
     1441 gagaagctga accacgtctt cgagaagttc taggcttcag gcagcacctt gaggaattct
     1501 tgggggaaca gagctaggga cgagagggcg atgcatacca tctgggcgct ccgaccgatc
     1561 cgaaaagcac tgtaggaggc aagcgtggag ggagtcaacc acatcgaggg gaacctatct
     1621 gccgagggca gatccttcgg gatagtcgcc tcgcgcttca acgattttgt cgtgaaggcg
     1681 ctgctggaag gggctatcga ggcgatccgg cgccacgggg gcgtagcgga ggccgtcgac
     1741 gtcgtctggg tgccaggttc ctacgagata ccggtcgtcg ccagagagct ggccctttca
     1801 ggacgctacg acgcactcat ctgcctcggg gccgtaatca ggggcgcaac ggcccacttc
     1861 gactatgtcg ccggaggggc cgccggtggc atctcgaacg tggcgctgga gaccggggtg
     1921 cccgtgatct tcggcgttat taccacggag tcgatcgaac aggccataga acgcgccggc
     1981 acgaagatgg gcaacaaggg tttcgaggcg gcggtttcgg cgatggagat ggctgacctt
     2041 atggcgaaac ttcgtgcacg cgcctcgcaa gacgacacgt ccaccacgct ctacaaccct
     2101 tgagggctag acgctcggcg aaggcgctcc cacggaggcc ttcgttacct tgaacctttt
     2161 gaggcatgag gtgcagaccc actcgcgccg tgtggccgag ccttcctgaa tacgaacctt
     2221 ctgcaggttc gggtctctgc gccggcgcgt cgccctcagc gagtgactgc gcgcgttgcc
     2281 gaagtctggc ttctttccgc acgaataaca aactttagcc acgaaacttc tcctgtgcta
     2341 ggataccggt cgaatttggg gaccccggaa agcacgatac gcaagccgtc tccggacgtc
     2401 gggcggtgat tgtaccagat tgccgacgtt agcctagagt gggagaggac cgctcacggg
     2461 ctggtctgcc ggaagcccga tgagaggagc gatggaaggt ctttatcgat ggcggtagag
     2521 gtggccctca tggtcgccgc ggcccacacg gccgtcaaag cgcaggtcgc cag
//
LOCUS       DATMJA010000324         2508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_694366, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000324 DATMJA010000000
VERSION     DATMJA010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694366"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VNT12_07800"
     CDS             <1..1184
                     /locus_tag="VNT12_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI46061.1"
                     /translation="HLTGNSWYAFGKMDDEAVFEVDKDAITERVLSDLGPCVRCPILD
                     LDSTAEIVARLPERIDPSRDQSWGYREDGTLSGWKVVKTREGRQEAELQEDREGLIER
                     LARSVRSGGAGVAAASGTAPGSRNVPATRYEDVGGMRETIDLVRESVELPITHPEIFR
                     KLHIRPHKGILFYGPPGTGKTLLARAVAHESGAHFVAVSGPEILNKFWGQSEAKLRSI
                     FNEARAKAPAIILFDEIDSFASSRDMMSESFEATLVSQLLSLMDGLNNLGRVCVIATT
                     NRPGALDLALRRPGRFDHEIEIGLPDAEARLHILGIHTREMPTDPDLDLDQIAGLTGS
                     YSGADLEALCREAALVCMRRTINLRDFEKRIASHQLSALSVTTYDFRTAVKRIGPSIR
                     R"
     gene            complement(1744..2028)
                     /locus_tag="VNT12_07805"
     CDS             complement(1744..2028)
                     /locus_tag="VNT12_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46062.1"
                     /translation="MRPRRQAGTGSSYGTTYSPSPLDRAGDGPFELVVAGETPGEDRK
                     EGVRIVSAFEEAGVTWWIESIDPWRFGWTEGEPWPSEEMRRRVRQGPPEA"
BASE COUNT          462 a          858 c          745 g          443 t
ORIGIN      
        1 accatctcac gggcaactca tggtacgcct tcggcaaaat ggacgatgag gccgtcttcg
       61 aggtggacaa ggacgctatt accgagcgtg tcctttcgga cctcggcccg tgcgtcaggt
      121 gccccattct ggacctcgac tcgaccgccg agatagtggc ccgcctaccc gagagaatag
      181 accccagcag ggaccagtcc tggggttaca gggaagacgg aaccctctcg ggctggaagg
      241 tcgtgaagac gcgggaaggg cgccaagagg ctgagttgca ggaggatagg gagggtctta
      301 tcgaacgcct ggcgcggagc gtgcgtagtg ggggagcggg ggtcgctgcg gcgagcggca
      361 cggcgccggg cagccgcaac gtgccggcga cccgctacga ggacgtaggc gggatgcgcg
      421 agaccataga cctggtccgc gagtcggtgg aactgcccat aacccaccct gaaatcttcc
      481 gtaagctcca catcaggccg cacaagggaa tcctcttcta tgggccgccg gggacgggca
      541 agacgctcct ggcgagggcc gtcgcccacg agagcggggc ccacttcgtg gccgtctcgg
      601 ggcctgagat cctcaacaag ttctggggcc agagcgaggc gaagttgcgc tccatcttca
      661 acgaggcccg cgccaaagcg ccggcgatca tcctcttcga tgagatagac tccttcgcct
      721 catcgcgcga catgatgagc gagagcttcg aggctaccct cgtctcccaa ctcctctccc
      781 tcatggacgg gctcaacaat ctgggcagag tctgcgtcat agccaccacc aacaggccag
      841 gggcgctcga ccttgccctc aggcgtcctg gccgcttcga ccacgagatc gagatcgggc
      901 tccccgacgc agaagcgcgg ctgcacatat tagggatcca cacccgcgag atgcccacgg
      961 accccgacct cgacctggat cagatagcgg gcctcacggg cagctactcc ggcgccgacc
     1021 tggaagccct ctgccgcgag gccgccctcg tgtgcatgcg ccgcaccata aacctccgcg
     1081 atttcgagaa gcgaatagcc tcccaccagc tctcagccct ctccgtcacc acctacgact
     1141 tccgcaccgc cgtgaagcgc ataggcccaa gcatccggcg ctagagctgt actactcagg
     1201 cgaaggaacg gccggttcgt tctctccgcg catcagtcgc cgccgcgcgg tctccctccc
     1261 tccgaccgcc ttgatcgtga tctgaagcgc ggaagtggag cgaccgatga ccttcgtggt
     1321 tgaggtagac gagctcgcat cctctagacg gcagctttga tttccacctc accttcgagc
     1381 ccgaccacag ggcggaacac cactgtcggc attgtcccac ctccacgaag gtgtggtctt
     1441 gtctgtcggt tcgcctcttc cgaccatcgc tatgtccctt caccctacca ggtgtgctcg
     1501 acgagagcct agcagaggca cctggattga tcagccagcc gcattggacg tcggttcttt
     1561 ggtgggttgc ctactgcctc cggttggccg atctcctcag agggcctgcc aggtagcgag
     1621 tctcagctcc attacgctat cgaccgccat agtaatgcga aacggtacgg ccgtcgtcgt
     1681 aaccggcatg gaccgatacg acgtcggtcg ctacaactgt acccgctggc gcctgttcgt
     1741 gtctcatgcc tcgggcggtc cctgccgcac tcgacggcgc atctcttcgg acggccaggg
     1801 ttcgccctcc gtccacccga aacgccaggg gtcgatgctc tctatccacc aagtgacgcc
     1861 agcctcctca aaggccgaca cgatgcgcac gccttccttc cggtcctcgc ccggcgtctc
     1921 ccctgctacc accagctcga aaggtccgtc ccccgcgcgg tccaggggag aaggcgagta
     1981 ggtggtccca taagaagaac ccgtcccagc ctgcctcctc ggcctcacgg gccaactccg
     2041 tgatcactcg ggcgtccacc cctaccccga agttgggcac gctcacgccg tagcgcacgc
     2101 tgtgctcctc accgatcgtt tcactgaggc tcggtagccg cgctgctgca agcatactgc
     2161 aggagtcgca cggccactag gggtatgaat tgttcgtcct tcctccacag cgctcgtgcc
     2221 cgccggggcc ttccccgcag cacttccccg atagtcggag accggctccc acaccagctc
     2281 ggcccactcg acgaccagcg ccgtcgtcct gaccggcgtc gaccgacacg gaggctgtcg
     2341 cgacaacagt cgccaccggc gcaccgaagt aggtagcttc tccgaagttg gctttggcag
     2401 cgggaggtgt ttgtgcttgg ggcacacgct atcctggaga ggatgcatag cgcaagccgt
     2461 aggaggtaga caccgtatgg ttacgatctc gaaggacaac gatgtggt
//
LOCUS       DATMJA010000325         2877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_754289, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000325 DATMJA010000000
VERSION     DATMJA010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754289"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1794
                     /locus_tag="VNT12_07810"
     CDS             142..1794
                     /locus_tag="VNT12_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HWI46063.1"
                     /translation="MKFLRKDQTLNKVEKIKQERHPLEVYGAIIETYSKDLSAMDAVP
                     GEVERLKWVGIYPQKQGGDAFMMRIKIPGGVLTPEQTRVIGKIAADFARGPVPNPHFG
                     NNFLDLTTRQDVQMHWIKMENVPEIWRRLEEAGITTVQACGDSARNVLCCPVSGLGHD
                     EAIDAYPVAQAISAYFTGNREYANLPRKFKMSVTGCLEDCAQAEINDVGMLPARMEDG
                     TVGFNLRVGGGLSDGPRMASDIDVFVRPEDAVEITRGIAQVFGELGDRENRWTARMRY
                     LVQELGPEGFREELAKRVSVGLTPAGEDLTKRYRGDHIGVHPQKRKGGSGTALYYVGL
                     NVPVGRMSGEQFEEAGRLAGEYGDGVRLATDQNLIFTGVPKDRLDELLAEPLLARYSP
                     KPGAFERGVVACTGSEFCRFGIVETKVRALEWAREMDERVGDPGQETVRMHFSGCSAS
                     CAQPQIGDIGFRGETAKTLDAIVEGVDIGLGGSLGKDAAFIDWVEGAKPAGEVPDALV
                     AIFERFEEERREGERFHEWARRKRNAELREALRKPVESGVGQ"
     gene            1794..>2877
                     /locus_tag="VNT12_07815"
     CDS             1794..>2877
                     /locus_tag="VNT12_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HWI46064.1"
                     /translation="MAGFDIREMMNDIDEAPGKVWFWDLEKAVIDADRCVQCGVCVAA
                     CPTDSIGIGDDDLPQLVKMCTGCSLCWDFCPRGGLQYESTWKITGGSNGKSIEGMGRV
                     EESYTARVRKRIDGVQDGGFVSALLISLLEAGEIDGALLARESATERWKGEAFLATTP
                     EEVRECAGSFYNQTLALGHVDFDAHDLPPNPRIAVVGTPCEIEGIKAMQARPWTWGSS
                     KVEAITLTIALLCTKSFNYEKLMLEEIRDKRNVDLDNVGRVDVIRGKMIVQDLEGVTI
                     FEEPIRDFHGAALKGCDECADFMGHAADISVGSVGSADGYSSVLLRSEEGLAAFDSVR
                     DGLELRPLDKPQALDKLDGLDKRVAFN"
BASE COUNT          580 a          816 c          999 g          482 t
ORIGIN      
        1 accacgacga ggatcccagg gccggacggt gggcaggtac cgaaaagacg gagtgcggca
       61 tccacgtcga aggcggccag atcagccgcg cgggcgcctg aatatcagag aatttctctc
      121 caagagcgca ggttctacaa catgaaattc ctcaggaaag accagacctt gaacaaggtc
      181 gagaagatca agcaggagcg ccatccccta gaggtttatg gggccataat agagacctac
      241 tcgaaagacc tctcagccat ggacgctgtc cctggcgagg tcgagcggtt gaagtgggtc
      301 ggtatctacc cgcagaagca gggcggcgac gcgttcatga tgcggatcaa gatccctggc
      361 ggcgtgctga cgcccgagca gaccagggtc ataggcaaga tagcggccga cttcgcgcgg
      421 ggccccgtgc ccaacccgca cttcggaaac aacttcctcg atctgaccac gcgccaagac
      481 gtacagatgc actggatcaa gatggagaac gtgcccgaga tctggcgccg cctggaagag
      541 gccggcatca cgaccgtgca ggcctgcggc gactcggccc gcaacgtcct ctgttgcccc
      601 gtctcgggcc tcggccacga cgaggctata gacgcctacc ccgtggcgca ggctatctcc
      661 gcctacttta ccggtaaccg cgagtacgcg aacctgccga ggaagttcaa gatgagcgtc
      721 acgggatgcc tggaggactg cgcacaggcc gagatcaacg acgtaggcat gctccctgcc
      781 cgaatggaag acggcactgt gggcttcaat cttcgcgttg gcggcggcct ctccgacggc
      841 ccgaggatgg cctcggatat cgacgtgttc gtcagacccg aagatgcagt ggagatcacc
      901 cgcggcatag cccaggtctt cggcgagctt ggggacaggg agaatcgctg gacggcgagg
      961 atgcgctacc ttgtccagga gctcggtcct gagggcttcc gcgaagagct cgcgaagcgc
     1021 gtttccgtgg ggctcacacc cgccggagag gacctgacga agaggtaccg cggggatcac
     1081 atcggcgtcc acccccagaa gcgtaagggt ggttctggaa ccgcccttta ctacgtcggc
     1141 ctgaacgtgc ctgttgggag gatgagcggg gagcagttcg aggaggctgg taggcttgcc
     1201 ggggagtacg gggacggggt ccgactcgct acggaccaga acctgatctt tacgggcgtt
     1261 cccaaagacc ggctcgatga acttctggcc gagccccttc tggcgcggta ctccccgaag
     1321 cctggggcgt tcgagcgtgg cgtcgtagcc tgcacgggga gcgagttctg ccgcttcggc
     1381 atcgtcgaga ccaaggtcag ggccttggag tgggcccgcg agatggacga gagggttggg
     1441 gatcctggcc aggagacggt gaggatgcac ttctcgggct gctcggcctc ctgcgcgcag
     1501 ccccagatcg gggatatagg tttccgcggc gagaccgcga agacgctgga cgcgattgtg
     1561 gagggcgtgg acataggtct tggcggctct cttggcaagg atgctgcgtt tatcgactgg
     1621 gtcgagggcg cgaagccggc tggggaggtc cctgacgccc tcgtggctat cttcgagcgg
     1681 ttcgaggagg agcggcgaga gggtgagcgc ttccacgagt gggccaggcg caagcggaac
     1741 gccgaactgc gcgaggcgct gcgcaagccg gtcgagagcg gggttggtca atagtggccg
     1801 gcttcgacat cagagagatg atgaacgaca tcgacgaggc tccaggcaag gtctggttct
     1861 gggacctgga gaaggcggtg atcgacgccg accgctgcgt gcaatgcggc gtgtgtgtcg
     1921 cggcctgccc taccgactct atcggcatcg gcgatgacga ccttccccag ctcgtcaaga
     1981 tgtgcaccgg ctgctcgctg tgctgggact tctgcccccg cggcggactg cagtacgagt
     2041 cgacctggaa gatcaccggc ggctctaatg gtaagagcat cgagggcatg ggacgcgtcg
     2101 aggagagtta cacggcccgc gtcaggaaga gaatagacgg cgtgcaggat ggtggcttcg
     2161 tcagcgcgct tctgatctcg ctgctcgagg ccggcgagat cgacggcgcc ctgttggcgc
     2221 gcgagagcgc gaccgaacgc tggaagggcg aggcttttct ggctacgacg cccgaggagg
     2281 tgcgtgaatg cgcggggagc ttctacaacc agacgctcgc gcttggacac gttgacttcg
     2341 atgcccacga cctgccgccg aaccccagga tagccgtcgt ggggacgccg tgtgagatcg
     2401 agggcatcaa ggcgatgcag gcgaggccat ggacctgggg cagctcgaag gtcgaggcca
     2461 taacgctcac catagccctg ctctgcacca aaagcttcaa ctacgagaag ttgatgctag
     2521 aggagatcag ggacaagagg aacgtcgacc tggataacgt gggacgggta gacgtcatac
     2581 gagggaagat gatcgtccag gatctcgaag gcgtaacgat cttcgaggag ccgatacgtg
     2641 actttcacgg ggcggccctc aagggttgcg acgagtgcgc ggacttcatg gggcacgctg
     2701 ccgacatctc cgtagggagc gtcgggagcg cggacggtta ctcaagcgta ctcctccgga
     2761 gcgaagaagg cctggccgcc ttcgacagcg tcagggatgg attggagctt cgtccccttg
     2821 acaagccgca ggctttggat aagctagacg gcttggacaa aagggtggcc ttcaata
//
LOCUS       DATMJA010000326         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_102432, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000326 DATMJA010000000
VERSION     DATMJA010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102432"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="VNT12_07820"
     CDS             complement(<1..2193)
                     /locus_tag="VNT12_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46065.1"
                     /translation="MVVFYDAGEEKEARKDALTLMEAGAATARVVEWAADAPHGTDVN
                     GRLVEDPEGFEEWLCGVIEGAKPLASDLTAKRTHRGGEPDSYGAEDEAASEDEAEERK
                     PTQAELLVRCASGADLFHTPAGDSYVTVPVRGHRETHPMKAKGFRRWLVRAYYERYGR
                     PPTNQALQDALTLLEARALFDGSEREVYVRVAGHAGNIYIDLVNEGWEVVEITPAGWR
                     VVSGEDTPVRFRRPRGMLALPTPLPVGDGDDGCDDLLRRFFNVSDEKELRLIVAWLVA
                     ALRPVGPYPALLFQGEQGSAKSTAERFARAVVDPSAAPLRTTPRNEHDLYIAADNAHV
                     IALDNISTLQPWLSDALCRLSTGGGFSTRTLYENREVELFEGMKPVILNGITDVATRP
                     DLLDRALIVSLPPIPDEERRAEAELWEEFEQLRPTILASLFDAVSGALRSVEDVRLEG
                     MPRMADFALWATAAEEFLGWDTGTFMTAYSGNRQEATDSALDADPVAAAVLDFMADRD
                     HWAGSASELWTALGELVYEGVRKTKAWPGAPNALTGRLRRLAPTLRGVGIEYGEYRSG
                     RSREKVLTKNKPARDRHDRHQRHDEEFSAKESQMRGDDPGDDLSVGDDVERHNDDPAQ
                     ETVTQQSLIDKGNAGGGDGRDGDDGDMQAESKAPLRDGRPLSEALEPGQSATLPELAA
                     WKRVEAAGAQMRCAKSGPALALKTYLEKPSAQRLEWLTKAVLRVLDEDT"
     gene            complement(2139..>2490)
                     /locus_tag="VNT12_07825"
     CDS             complement(2139..>2490)
                     /locus_tag="VNT12_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46066.1"
                     /translation="LAVHYKSKARNDDNKRWQKWGDYADDLPPKGHGKKPDDPAKVIP
                     FGMETLEGLEPGAQVILFCGEEDALSAARPATLRYPRRGRGSWSRPTPESSRASRWSS
                     STTPARRKRRERTR"
BASE COUNT          399 a          842 c          749 g          500 t
ORIGIN      
        1 cgtatcctcg tcgaggaccc tgagtaccgc cttcgtcagc cactccaagc gttgggcact
       61 aggcttctct aggtaggttt ttagtgccaa agccggcccc gacttcgcgc agcgcatctg
      121 tgcaccggcg gcttcgacac gcttccaggc tgccagctcc ggcaacgtgg cggactggcc
      181 gggctcgagc gcctcagata gtgggcgtcc gtcgcgaagg ggtgccttag attcagcctg
      241 catatcaccg tcatcaccgt cacgaccgtc accgccgcca gcattccctt tatctatgag
      301 gctttgctgt gtgacggtct cttgtgcagg gtcgtcattg tgacgctcta catcgtcacc
      361 aactgaaaga tcgtcaccag gatcgtcacc acgcatttgg ctttccttgg cggaaaactc
      421 ctcatcgtga cgctgatgac ggtcgtgacg gtctcttgcg ggcttgttct tggtgagcac
      481 cttctcgcgc gagcgtccgc tgcggtattc gccgtactct atgcctactc cccgcaacgt
      541 gggtgccagg cgcctgaggc ggccggtgag agcgttgggc gccccgggcc aggctttggt
      601 cttacggaca ccttcgtaca cgagctcacc cagggccgtc cacagctcgg aagcactccc
      661 cgcccaatgg tctctgtcgg ccatgaaatc tagcacggcg gcggcgacgg gatcggcgtc
      721 gagggcgctg tcggtagctt cctgccggtt gcctgagtag gctgtcatga acgtccctgt
      781 atcccagccc aaaaattcct cagcggccgt ggcccagagc gcgaagtccg ccatcctcgg
      841 cataccttcg agtcgtacat cctcgactga acgcagcgcc cccgatacag catcgaagag
      901 ggatgccagt atggtggggc ggagctgctc gaactcttcc cacagctccg cttctgccct
      961 gcgctcctcg tccggaatcg ggggcagcga gacgatgagc gcccggtcca gaaggtccgg
     1021 cctggtcgct acgtctgtga tgccgttcaa gatcacaggc ttcatgccct cgaagagctc
     1081 cacttcgcgg ttctcgtaga gcgtgcgggt ggaaaagcct ccgccagtag agagccgaca
     1141 caaggcatca gagagccagg gctgcaaagt gctgatgttg tccagggcga tgacgtgggc
     1201 gttgtccgcc gctatgtaga ggtcgtgctc gttgcgcggc gtcgtcctga gcggcgccgc
     1261 tgagggatcg acgactgcac gggcaaacct ctccgcagtg ctcttggcac ttccctgttc
     1321 tccctggaat agaagcgcag gatacggccc cacgggacgc agcgccgcga cgagccaggc
     1381 tacgatcagg cgcaactcct tctcatccga gacgttgaaa aagcgccgca agagatcgtc
     1441 acagccgtca tcgccgtcac ccaccggtag cggggtaggt agcgccagca tgccccttgg
     1501 cctcctgaat cgcacagggg tgtcttcgcc tgagaccacg cgccagcctg cgggcgtgat
     1561 ttccaccacc tcccacccct cgttaactag gtcgatgtag atgttcccgg cgtgtccggc
     1621 tacacggacg tagacctcgc gctcggagcc gtcgaacaat gcacgggcct ccagcagcgt
     1681 gagcgcgtcc tgtaaggcct ggttggtagg cggacggccg tagcgctcgt aataggcgcg
     1741 cacgagccac cgcctgaagc ccttcgcctt catcggatgc gtctcgcggt ggccgcgcac
     1801 cggaaccgtg acgtaggagt cgcccgccgg tgtatggaac aagtcagccc cgcttgcgca
     1861 ccggacgagg agctccgcct gtgtgggctt gcgctcctcg gcctcgtctt cagaagcggc
     1921 ttcgtcttcc gcgccgtagc tgtctggctc tcctccccga tgggtcctct tcgccgtcag
     1981 gtcggaggct agcggcttcg ctccttcgat cacgccgcag agccattcct cgaaaccctc
     2041 ggggtcctcc acgagccggc cgttgacgtc cgtcccgtgc ggtgcatccg ccgcccactc
     2101 gaccactcgt gccgtcgcgg cgcctgcttc catcaaagtc agcgcgtcct ttcgcgcctc
     2161 tttctcctcg ccggcgtcgt agaagacgac cacctcgagg cccgcgaact ctctggcgta
     2221 ggccggctcc aggagccccg cccccgcctg ggataccgaa gtgtggccgg cctggcggcg
     2281 ctcagagcgt cctcctcgcc gcagaacagg atcacttggg ccccaggttc gagaccctcc
     2341 agggtctcca tgccaaacgg gatgaccttg gccgggtcgt cgggtttctt gccgtgcccc
     2401 ttcggcggca gatcgtcggc gtagtctccc catttttgcc aacgtttatt gtcgtcgttg
     2461 cgggctttgc tcttgtagtg aaccgccaac
//
LOCUS       DATMJA010000327         3151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_221492, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000327 DATMJA010000000
VERSION     DATMJA010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221492"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VNT12_07830"
     CDS             complement(<1..443)
                     /locus_tag="VNT12_07830"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HWI46067.1"
                     /translation="MRAAIFLNGSPDSSDLLRRVAGLADLIVAADGGARYALEAGVVP
                     DLVVGDMDSLGEDLAREVVKLGGSLERHPVRKDKMDGQLAVLAARARGATTADLFCAV
                     GGRLGAFFAVPQILLAAERIGLRSTVVANRGRIFVIEAGSRTVQG"
     gene            complement(445..1002)
                     /gene="thiT"
                     /locus_tag="VNT12_07835"
     CDS             complement(445..1002)
                     /gene="thiT"
                     /locus_tag="VNT12_07835"
                     /inference="COORDINATES: protein motif:HMM:NF021062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HWI46068.1"
                     /translation="MNAFRDTRVLTEAALAVGLAFVLGFVVLFRMPFGGSVSLEMIPL
                     ILLALRQGWKVGVVAGAVYGLLDLAIDPYVVHPVQFILDYPLAFGALGLAGLFKPTVR
                     GAVLGATVAVLARFACHFLSGVVFFASVAPEGWNPYLYSAAYNAAYLAPSLGIVLVVT
                     VVLLKALERAQPSPRQLYYRGSKTS"
     regulatory      complement(1039..1141)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1147..1725)
                     /locus_tag="VNT12_07840"
     CDS             complement(1147..1725)
                     /locus_tag="VNT12_07840"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI46069.1"
                     /translation="MAFILPRMGAESWRTLAREYFYQSPWCAFRVDEVELPGGARIEY
                     GVLESGGFAAVVAVTGGGDVVLVRQWRQPLGAFTLELPSGGVDADEAPERAAHRELFE
                     ETGYRAEGLEHLVSVHTSTGRSTEVCHLFRCAAVRDERGPRPEPTEFIRVVELPLGKA
                     LQMVSEGGITDAATVLGLLWSTAGGASGGGLH"
     gene            complement(1885..2118)
                     /locus_tag="VNT12_07845"
     CDS             complement(1885..2118)
                     /locus_tag="VNT12_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWI46070.1"
                     /translation="MTYVITEPCIGTKDQSCVEVCPVDCIYDAGDHFMINPEECIDCG
                     ACEPECPVEAIYPEDEVPDDMESYTTKAADYFE"
     gene            complement(2268..3020)
                     /locus_tag="VNT12_07850"
     CDS             complement(2268..3020)
                     /locus_tag="VNT12_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWI46071.1"
                     /translation="MYGENAPPSWARLPGARCRAVEHLTKLDGLKEFELFEGLEEGFF
                     QDLAIAAEVLELGADTALYREGEPSGAVYFVGEGRVKLYRTSGGPKARDQILGVVGDG
                     SVLGLGAAIEGRTQGQGATALTDCVLYAVFRDDLFEIMGRFPDGAVRLSRALAARTRE
                     LEDLVGDLVFRSAPQRVARMLLNLATEEGRVTKRGVVFAPSLSRQEMAEATGISREAL
                     SRALSRLAKEGILDLGGKTITILMPAELRDRT"
BASE COUNT          607 a         1062 c          935 g          547 t
ORIGIN      
        1 tctccttgca ccgtcctgga gccggcctcg atcacgaaaa tccgccctcg gttcgccacg
       61 acagtggaac gcaggccgat gcgctcggcg gcgagcaaga tctgtgggac cgcgaagaag
      121 gcccccaacc tcccgcccac ggcgcagaaa aggtcggctg tggtcgctcc ccgcgccctg
      181 gcggcaagta cggcgagctg gccgtccatc ttgtctttcc tcaccggatg acgctccagg
      241 gagccaccga gtttcacgac ttctcgggcg agatcttctc ccaacgagtc catatccccg
      301 acgacgagat caggcacaac cccggcctcg agcgcgtaac gggccccacc gtcagcggcg
      361 acaatgaggt ctgcaagtcc ggcgacgcgc ctgagcagat ctgacgagtc aggagatccg
      421 ttcaggaaga tggcagcccg cacgctacga ggttttcgag ccccggtagt acagttgtct
      481 tggcgaaggc tgcgcccgtt ccagagcctt cagcagcacc acagtgacca ctagcacgat
      541 gcccaggcta ggggccagat acgcggcgtt gtacgctgct gagtagaggt aagggttcca
      601 gccctcgggc gcgacggagg cgaagaagac cacgccagag aggaagtggc acgcgaagcg
      661 cgccagaacg gccactgtgg cgccaaggac ggcaccccgc accgttggtt tgaagagtcc
      721 tgcgaggcca agggcgccga aggccagagg gtagtcaagg atgaactgca caggatgtac
      781 cacgtaagga tcgatggcga gatccagcag accgtaaacc gctcccgcga cgacgcccac
      841 cttccagccc tgcctgaggg cgaggagtat aagggggatc atctccaggg agacggagcc
      901 gccaaatggc atcctgaaga gcacgacgaa tccgagaaca aaagccagcc cgacggcgag
      961 cgccgcctcg gtcagtaccc tggtgtctct gaaggcgttc aattgcgcat cctccttccg
     1021 gtctacgggg acgcgccgat aagagactcc gggatctccc tgcgccggaa ttacccggat
     1081 caggttcagc gggtcggcac ctcggtgccc tctcagcctc acggctcccc ggtcttgctg
     1141 ttgcccctag tgtaacccac ctccggaagc accaccagcc gtgctccaga gcaacccgag
     1201 gacggtggcg gcgtcggtga tcccaccttc agacaccatc tgcaaagcct tcccaagcgg
     1261 caactccacc acccttatga actctgtagg ctcgggccgc ggaccacgct cgtccctcac
     1321 cgccgcacac ctgaaaagat ggcagacctc cgtgctccgc cccgtgctgg tatgtacgga
     1381 gacgagatgc tccaatccct cggccctata gcccgtctcc tcgaagagct ccctatgagc
     1441 cgcccgctcg ggcgcttcgt cggcgtcgac cccgccgctc ggaagctcca gcgtgaaggc
     1501 gccaagcggc tgcctccact ggcgcacgag caccacgtct cccccgcccg tgaccgctac
     1561 tacagcggcg aacccgccgc tctcaaggac accgtattcg atccgtgccc cacctggcag
     1621 ctccacctcg tccacacgga aggcgcacca aggactctgg tagaagtact cccttgcgag
     1681 cgtccgccag ctctctgctc ccatgcgggg aagtataaat gccatgcgca caggtattgg
     1741 cccaccgcga atcgcgcatt cgaatacgag caacaagaaa atgctgaagt gctgaaaagc
     1801 ggaggcatca gccggagcgt gctgacatgc ttacaaaaag gaggggcctg agcccctccc
     1861 gaatactgcc tcatgccctg atttctactc gaagtagtcg gcggccttgg tggtgtagct
     1921 ctccatgtcg tcggggactt cgtcctcggg gtagatggcc tcgaccgggc actcgggttc
     1981 gcaggcgccg caatcgatac actcttcggg gttgatcatg aagtggtcgc cggcgtcgta
     2041 gatgcagtcg accggacaga cctcgacgca actctggtcc ttcgtgccga tacacggctc
     2101 cgttatcaca taggtcaaaa gggcctcctc tctgaatctc gtaccggctt tataccagcc
     2161 ggcccccacc cccaacaccc gccgttgacc ggcgtcatca cagcccatga ttagggtcat
     2221 acggtcgtgt gaccttggtc aaaaacggcc cttgctgcga gccgttctca ggtacggtcg
     2281 cggagttcgg cgggcatgag tatggttatg gtcttgccgc cgaggtccag gatgccctcc
     2341 ttggcgagcc gggagagggc gcgtgagagg gcttcgcgcg agatgcccgt cgcctcggcc
     2401 atctcctgcc tgctgagaga aggcgcgaag acgacgcccc tcttggttac gcggccctcc
     2461 tctgtggcga ggttcagcag catccttgcc accctctgtg gggcgctgcg gaagaccagg
     2521 tcgccgacga ggtcctctag ctcgcgggtc ctcgctgcca gcgccctgga gagcctgacc
     2581 gcgccgtcgg ggaagcggcc catgatctcg aacaggtcgt cgcggaagac ggcgtatagc
     2641 acgcagtcgg taagagcagt ggcgccctgg ccctgggtcc tgccttcgat ggccgccccg
     2701 aggccgagca cggagccgtc gccgaccacg ccgaggatct gatcccgcgc cttcggtccg
     2761 cccgatgtac ggtagagctt cactcgcccc tcaccgacga agtacacggc ccccgaaggc
     2821 tcgccctccc tgtacagggc ggtgtctgcg cctagctcga gcacctcggc cgctatcgcg
     2881 agatcctgga agaatccctc ttccagccct tcgaaaagtt cgaactcctt gagtccgtcc
     2941 agcttcgtca ggtgctcgac cgcccggcaa cgcgcgccgg gcaaccgcgc ccaggaaggc
     3001 ggtgcgttct caccgtacat cgttatgtgt tccttccggc gcacgtgatc tcacagaccc
     3061 tttatacgct aagtggggat aatttgcact gagagcgctt caggcatcat gtttcaggaa
     3121 ttggctgtga tgctagcgac agtaactatt c
//
LOCUS       DATMJA010000328         2890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_635797, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000328 DATMJA010000000
VERSION     DATMJA010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635797"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1861
                     /locus_tag="VNT12_07855"
     CDS             182..1861
                     /locus_tag="VNT12_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HWI46072.1"
                     /translation="MKTNFDAYLRYGELTRALHAFAEDHPGFCKVESIGKSYEGREIW
                     LAELTNLKTGPAETKPAFWVDGNTHAGEVTGSMAALYLIEHLLEKHGSEDLPTRLLDE
                     QAFYVLPRLSPDGAERFLTTAYTLRATLRPWPEPEEEPGLHPEDVDGDGNILQMRIVD
                     AGGEWRVSDKDPRLMVPRAPDEADPNTTYYRLYREGTFKDYDGFGRRIARPLYGLDMN
                     RQYPYHWQAEDEQKGAGPYPLSEPESRAQVDFLLGHKNIFGVHTYHTFCGAILRPYSN
                     RPDDEMPEHDLAVFKALGKRGTQITGYPNISVYHDFRYDPKKPITGAFDDWAYDSYGV
                     FAFTIEFWSMARAAGVEVKDFIEFFRDPPEEASLKMLAWNDRELGGDGFVSWRPFDHP
                     QLGRVELGGWKTKFTAQNPPPKFLRAECEKLTHFALSHAATAPRLRTRLETEELSQGL
                     RRIELVVENAGYLPTHVTSVAADKKLTRPVEVGIELPQGASLVSGEWEVELGHLAGRS
                     ALAGNRWKSPAFFEGLPSDYASRTVWVVRGEGPVHVEVRGGRAGTARLETK"
     gene            complement(2049..2249)
                     /locus_tag="VNT12_07860"
     CDS             complement(2049..2249)
                     /locus_tag="VNT12_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46073.1"
                     /translation="MLKERFESIPVNVPRSEIEAMGVEIGLDDPTEAARIFDRLKGIS
                     WRGDYVRSEEGWVAAWVVEVIC"
     gene            complement(2418..>2890)
                     /locus_tag="VNT12_07865"
     CDS             complement(2418..>2890)
                     /locus_tag="VNT12_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI46074.1"
                     /translation="LVVFFGSLLVPLVILLAVPLTTVGAFGAMYLTNTAVSVPSLLGI
                     LLLIGIVVSNAILLVDFAIKGRHRYATTDEAILAAGQARLRPILMTAFATIFALMPLA
                     LGLSGGGNGLISSSLAITVVGGLATSTFLTLLVVPVGYSLLKSGRRRKEKAKKG"
BASE COUNT          595 a          920 c          887 g          488 t
ORIGIN      
        1 gaccctggaa tgcgataagt atatatgccc gaagacgctc caggtacact accagagagc
       61 gactgtgacg ttacccaaca tgctggagcg tgtcaaactt gcatgccaca tctagtggct
      121 catttcagcc caaaggggcc atcttgggcg gcgtttcttg cacatggagg agaagtagag
      181 catgaagaca aacttcgacg cttacctgcg atacggggag ctcacacggg ccctgcacgc
      241 gttcgcggag gatcaccccg ggttctgcaa ggtcgagtcc ataggcaaga gttatgaggg
      301 gcgtgagatc tggctcgcgg agcttaccaa cctaaagaca ggccctgccg agacgaagcc
      361 tgccttctgg gtagacggca acacacacgc cggcgaggtt acgggctcga tggcggcact
      421 ctacctgatc gaacacctcc tcgaaaagca cggctcggaa gacctcccga cccgcctcct
      481 cgacgagcag gccttctacg tgctcccccg tctctccccc gacggcgccg agcgcttctt
      541 gacgacggcc tacaccctgc gggcgactct gagaccctgg cccgagcccg aagaggagcc
      601 tggcctccac cccgaggacg tcgacggcga cggcaacatc ctccagatgc gcatcgtcga
      661 cgctgggggc gagtggcgcg tctccgacaa ggaccccagg ctcatggtcc cccgggcccc
      721 cgacgaagcc gaccccaaca ctacatacta ccggctctac agggaaggga ccttcaagga
      781 ctatgacggc ttcgggcggc ggatagcgcg tcccctctac ggcctcgaca tgaaccgcca
      841 gtacccctat cactggcagg ccgaggacga gcaaaagggc gccggaccct acccgctctc
      901 ggagccggag tcgagggcgc aggtcgattt cttgctcggg cacaagaaca tcttcggcgt
      961 gcacacctac catacgttct gcggtgcgat cctgaggccc tactccaaca ggccagacga
     1021 cgagatgccc gagcacgacc tcgctgtttt caaagctctt ggcaagcggg gcacacagat
     1081 cacaggctac ccgaatatct ccgtctacca cgacttccgc tacgacccga agaagcccat
     1141 aaccggcgcc ttcgacgatt gggcttacga ttcctacggt gtcttcgcat ttaccatcga
     1201 gttctggtcc atggcgcggg ccgccggtgt agaggtgaag gactttatcg agttcttccg
     1261 cgacccgccc gaagaggcgt cgctgaagat gctcgcgtgg aacgacaggg agctcggagg
     1321 tgatggcttc gtctcctgga ggcccttcga ccaccctcaa ctcggacgcg tggagttggg
     1381 gggctggaag accaagttca cagcccagaa tccgccgccc aaattcctca gagcggagtg
     1441 cgagaagctt acccatttcg ccctctccca tgcagccacg gccccgcgcc tgcggacccg
     1501 cctggagaca gaggagctct cccaggggct ccgtcgtatc gagctggtcg tcgagaacgc
     1561 cggttacctg ccgacgcacg taaccagcgt agcagccgac aagaagctca ccaggcccgt
     1621 cgaagtcggg atagagttgc cgcaaggcgc gagcctcgta tccggagagt gggaggtcga
     1681 gctgggacac ctcgccggac gttcggcgct cgccggcaac aggtggaaga gcccggcctt
     1741 cttcgagggg ctgccctcgg actacgccag ccggacggtc tgggtcgtgc ggggcgaggg
     1801 acctgtccat gtcgaggtgc gcggaggaag ggcaggtacg gcacggctcg aaacgaaata
     1861 gcgtgtcagc aaatcgtccc gttgccttgc cacgagcgtc gtacccgacc gcctagggca
     1921 gcggcacctc gtgcgcggca gggcgaggca gcaacagaaa aagctgaaat gctgacgagc
     1981 gaggccgcag gccgaagagt gctgagaggc ggcggagccg ccgtgctgac atgctgaagt
     2041 tcaggacttc agcagatcac ttctacgacc caggcggcga cccagccttc ttccgacctc
     2101 acgtagtcgc ccctccacga gatacccttg agcctgtcga agatcctggc cgcttctgtc
     2161 gggtcgtcga gcccgatctc gacgcccatc gcctcgatct cgctacgcgg aacattgacc
     2221 gggatgctct cgaacctctc cttaagcacc tcgcgcatcc tgatctgctg ctgcctgtcg
     2281 ggggtccgat atctgccttc tctgatggcc gacctcctct tcgtacaagg acagtctgcc
     2341 cttccccaag gaatgaaact gtcgtacaaa acactccgta aggtctaagt tcggcagatc
     2401 tgtagacaag cgttaactca tcccttcttt gctttctcct tccgtctacg gccgctcttg
     2461 aggagcgagt agccgacggg cacgacgagc agcgtcagga acgtcgaggt cgcgaggcct
     2521 ccgacgaccg ttatggcgag gctcgatgag atcaggccgt taccgccgcc ggagagcccg
     2581 agggcgagtg gcattagggc gaagatggtg gcgaaggccg tcatcaagat gggcctgagc
     2641 cgcgcctgac cggccgccag gatggcctca tctgtggtcg cgtaacggtg gcgtcctttg
     2701 atggcgaagt cgacgagcag gatggcgttg gagaccacga tcccgataag caagaggatg
     2761 ccgagcagcg acggcacgga gacggcggtg ttcgtgaggt acatcgcgcc gaaggccccg
     2821 acggtcgtga ggggcacggc gagcagtatc accagcggaa cgagtagcga gccgaaaaac
     2881 actaccagca
//
LOCUS       DATMJA010000329         3427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_873111, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000329 DATMJA010000000
VERSION     DATMJA010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873111"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..443
                     /locus_tag="VNT12_07870"
     CDS             150..443
                     /locus_tag="VNT12_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46075.1"
                     /translation="MISYYGGDASGLGDEFHVNRQQPVSARVESLPLRDAPAWTLERL
                     VDVGLRWLTSGRLRTEGIVTPVVPFEESVEAYREIDERPERSIKLGISFPRAQ"
     gene            complement(834..2258)
                     /gene="menE"
                     /locus_tag="VNT12_07875"
     CDS             complement(834..2258)
                     /gene="menE"
                     /locus_tag="VNT12_07875"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HWI46076.1"
                     /translation="MRDETPSVPCPLRSAALAAPKTAALVGASGITSYEELDRMVAAA
                     ALHLGELEPGSRVALHLPKDERYVALVLALIRAGHVACPVSDRLPQQGVALLLERAAC
                     SALISEDQELLQTAGVGLLRPAPEALLREGKQRAEPADFQPERSATIIFTSGSSGVPK
                     AALHTFGNHYYSALGSNANIALQPGDRWLHSLPLYHVGGLSILFRCLLAGATVALPQP
                     GTSPGESITSLGATHVSLVSTQLSRLLRESADLGRLQAVLMGGGPVPPSLVDEALAHE
                     LPLYTSYGLTEMASQVTTTPPGACPEQLRTAGRVLPRREVSVSERGEILVRGETLFAG
                     YAEGEELDRPVDEEGWFHTRDFGELDESGYLRVGGRMDNLFISGGENVQPEEIEEALC
                     RLEGIDEAVVVPVPDEEFGARPVAFVRISDAEPRDLARELEPVLPRFKIPISFHPWPE
                     EARRGMKADRAALGEQARRLRSGV"
     gene            complement(2263..>3427)
                     /locus_tag="VNT12_07880"
     CDS             complement(2263..>3427)
                     /locus_tag="VNT12_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI46077.1"
                     /translation="SSPRRSGATGSPSSPPAQRVRLALARLLLEDHDLILFDEPTNHL
                     DVPAREWLEGHLAGMDAAYVVASHDRRFLDAVSRKVGHLDRGELTLYSGDYTAFRQQL
                     RQAEEEGWRRYEKSRKLAKKLQRQAQDYQRWSDAGERKKRGAADKGFVGHKAAKVMKH
                     SLVARRRMEEAVENARTEKPFEKDAVKIEFGSSQGRSFVRAENLVVGYAKERPLTTEL
                     SFDLWAGDRLCILGPNGCGKTALLRTALGEIPPLRGDVRLAPSSKVGYFDQDNRLVPP
                     DVTALEAVLATGRDETLVRTVMGRMGVRRETVNKKVAKLSSGERAKVLLAGLILGDNN
                     LLVLDEPTNYLDIETQDVLLEALRDFPGGILFVSHDRYFVEELATETLTPGLT"
BASE COUNT          599 a         1100 c         1072 g          656 t
ORIGIN      
        1 ggtccgcctt cctctgtcgc catgaaatgt tcgtgtgtca ggtctgctaa cttatgacaa
       61 gaatcggcaa aagtcgttgg acgtatgggg ggagcattgc tgcgctgcat catgccatcc
      121 gcgccacgag gttcggaggc acgatctgca tgatctccta ctacggagga gacgccagcg
      181 ggctcggcga cgagtttcac gtcaaccgcc agcagcccgt ctcagcccgc gtagagagcc
      241 ttccgctgcg cgacgccccc gcatggacgc tggaaaggct ggtcgacgtc gggctgcgct
      301 ggctgacttc aggccgtctc cgtacagaag gtatcgtgac gccggtggtg cctttcgagg
      361 agagtgtcga ggcataccgt gagatcgacg agcgccccga acggtccatc aagctcggca
      421 tcagcttccc acgagcacaa taggtgacgg tatggaactg ctgtgcttat gactcacttt
      481 cccatcacaa agtcgtatct agccaccgcc gcgttggctg gtcggcttta agatgagtat
      541 gatctcgccg atgttcgctg tcggctgatt tcggcaacgc tgctgatgtt caactcgatg
      601 ccgtcgaatc caatgccttc cagccaggct atttgtccgg ccaactcatc atccctgctc
      661 ttgtactgtt tacaccccca tggatcaagt accaagatct ccgagacgca gcgaagagcc
      721 gcctgtacag gcccgtgccg gccacagggc gctcgaactg gctcatgccg ctgtcgcatc
      781 tttcgagacc ggaaggcgcg tggccacgat ctgatccagg acagcgagcc ctctcagact
      841 ccgctacgca accgccgggc ctgctcaccc agggccgccc tatctgcctt catgcctcgt
      901 cgtgcctcct caggccacgg atggaaggag atggggatct tgaaccgcgg caagacaggc
      961 tcgagctctc ttgcaagatc tcttggttct gcgtcactta tcctgacgaa ggcgacaggt
     1021 ctcgccccga actcctcgtc gggcacaggc acgaccaccg cctcatcgat cccctccaac
     1081 cggcagaggg cctcctctat ctcctcgggc tggacgttct cgccgcccga gataaagagg
     1141 ttatccatgc gtcctccgac ccgcaggtag ccgctttcgt cgagctcgcc gaagtctctg
     1201 gtatggaacc aaccttcctc gtccacagga cggtctaatt cctcgccctc ggcgtagccc
     1261 gcgaagagcg tctcgccccg gaccaggatc tctcctcttt cggagacgga gacctcgcgc
     1321 ctgggcaata cacgacctgc ggtacgcaac tgctcagggc aggcacccgg cggtgtcgtc
     1381 gtgacctgcg aggccatctc cgtgaggccg tagctggtgt agagcggtag ttcgtgggca
     1441 agggcctcgt ctacgagcga gggcgggacc ggacctcccc ccatgaggac ggcctgcagc
     1501 ctccccaggt cggcgctctc ccgcagcagc ctcgagagct gggtagagac gagcgagacg
     1561 tgcgtggcgc cgaggctggt gatggactcg ccgggtgaag tcccaggctg tggaagcgca
     1621 accgtcgccc ccgcgagcag gcaccggaac agtatggaaa gccccccgac gtgatacagc
     1681 ggcaacgaat ggagccacct gtcgccgggc tggagcgcga tgttggcgtt cgagccgagt
     1741 gcgctgtagt agtggttgcc gaacgtgtgg agcgccgcct tcgggacgcc gctgctccct
     1801 gaggtgaaga tgatggtcgc agaacgctcg ggctgaaaat ccgccggctc cgcacgctgc
     1861 ttgccttctc ggagcagagc ttcaggcgct ggcctgagca ggccgacgcc tgccgtttgc
     1921 aggagttctt ggtcttcgga gatcagggcc gagcaggccg ccctctcgag gagcagcgcg
     1981 acgccttgtt gtgggaggcg gtcgctgacg gggcaggcta cgtgtccggc acgaatcagg
     2041 gcaagcacca acgccacgta tcgctcgtct ttgggtaaat ggagcgccac ccgacttccc
     2101 ggttcgagct ctccgagatg gagcgccgcg gccgcgacca tccggtccag ctcttcgtaa
     2161 gaggtaattc ctgacgcacc gacgagggcg gcggttttcg gagcggcgag agcagcggac
     2221 cgcaaaggac agggtactga tggagtctcg tctctcacgg cactatgtta gcccgggcgt
     2281 cagggtctct gttgcgagct cttccacgaa gtagcggtcg tgggagacga acagtatccc
     2341 gccggggaag tctctgaggg cttcgagtaa tacgtcctgg gtctctatgt cgagataatt
     2401 cgtgggctcg tcgaggacga gtaggttgtt gtccccgagg atgagcccgg ccaggagtac
     2461 cttggcgcgc tcgccggagc tcaacttcgc caccttcttg ttgacggtct ccctcctgac
     2521 acccatcctg cccatgacgg tacggaccag ggtctcgtcg cgaccggtcg cgaggaccgc
     2581 ctccagggcc gtgacgtcag gaggcacgag gcggttgtcc tggtcgaagt agccgacctt
     2641 cgaggagggt gcgagacgaa cgtcgccgcg caggggtggg atctcaccga gcgcggtacg
     2701 caggagcgcg gtcttgccgc aaccgttcgg acctaggata catagcctgt ccccagccca
     2761 gagatcgaag gacagctctg tggtcaaagg acgttccttc gcgtaaccga ccacgaggtt
     2821 ctcggcgcgc acgaagctcc ggccctgcga ggacccgaac tctatcttga ccgcatcctt
     2881 ctcgaacggc ttctccgtcc tggcgttctc gacggcctcc tccatcctgc ggcgggcgac
     2941 gagagaatgc ttcatgacct tcgcggcctt gtggccgacg aaccccttgt cggccgcacc
     3001 ccgcttcttc ctctcgccgg cgtctgacca cctctggtag tcctgggcct gccgctgcag
     3061 cttcttcgcc agcttccggc tcttctcgta cctgcgccat ccctcctcct cggcctgcct
     3121 cagttgttgc ctgaaggcgg tgtagtcgcc agagtagagg gttagctcgc ctcggtcgag
     3181 atgacccact ttcctggaga cggcatcgag aaagcgcctg tcgtgggagg ccacgacgta
     3241 ggcggcatcc atccctgcga ggtgcccttc gagccattcg cgggcgggga catcgaggtg
     3301 gttggtcggc tcgtcgaaaa ggatgaggtc gtgatcctct agcaacagcc tcgcgagcgc
     3361 gagccgcact ctctgagccg gcggagagct cggagaccct gtggccccag aacgccgcgg
     3421 agaggac
//
LOCUS       DATMJA010000330         4304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_162815, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000330 DATMJA010000000
VERSION     DATMJA010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162815"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VNT12_07885"
     CDS             <1..789
                     /locus_tag="VNT12_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWI46078.1"
                     /translation="PRQTPPGGGSPGMNERPALLLEEVYKSFGENEVLKGIDLTVEPH
                     EVVCLIGASGSGKSTLLRCINLVEPIDSGRVVVDGEEITAQGVDANRIRRRIGIVYQS
                     FNLFPHMTVLRNITLAPREVLGTDRAEAEKNARALLERFGLLDKQDEYPDRLSGGQQQ
                     RVAIVRALAMRPHLMLLDEVTSALDPELVAEVLAVIKELAREGMTMLIATHEMGFARD
                     IADRVCFLEAGRILEQGKPERIFTAPEHPRTRQFLGRIIEAGRL"
     gene            complement(1182..1370)
                     /locus_tag="VNT12_07890"
     CDS             complement(1182..1370)
                     /locus_tag="VNT12_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HWI46079.1"
                     /translation="MPETRKITWEPEADRRIKRSPFLMRRFIRKRIEDRVASRGRTHV
                     TEADVDESASMYRRYRFK"
     gene            1508..2470
                     /locus_tag="VNT12_07895"
     CDS             1508..2470
                     /locus_tag="VNT12_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI46080.1"
                     /translation="MRPETENAPPALEAKNISRLYKSSGRGVSDVGLRVERGEVFGLM
                     GPNGSGKSTLLRVLSTATAPDSGSFFVGGVDGLKEKREVRARMGLMVDRPTHYDDLTG
                     RANAYFFARAYGMEEAKADRALAELFEYFDLAEYANDKVKTYSYGMGKKLAMIEALAH
                     GPEVLLLDEPSLGLDYTSQLAYQSRIRDLAGSGVAVLLASNQVDEVEALCDRAAFLHR
                     GEVVAEGTPEDLISRVEGIRRIVATLRNPVEYGRVAEVGGVVRVVPEGRDLIFHCEER
                     PGIVADVVAGIVRSGGDIVNLSVQRPSLEDVFVELTGEALRDEV"
     gene            2460..3182
                     /locus_tag="VNT12_07900"
     CDS             2460..3182
                     /locus_tag="VNT12_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI46081.1"
                     /translation="MKFDLPDAYWKKDVALFFSRKFALILKMLYPMAIIVPVAASGMP
                     AKYAAAVIGIVAVMAGTFGAGESLTIDLNDGILVRVALTPLSPYRIVFEILAVNAVLD
                     LMQLLPALFIVYLLHPVSVGWVFAATLAIFATLVTANCIGIFIANFTSSPADVLLYAA
                     AILFPLIYLSGFFRNQHPTGILQTLRGFIPFAYTDDALRTVFGMSTGTTALEILVYPT
                     IICAVIAGAVCLTAPRLLSQRM"
     gene            3300..>4304
                     /locus_tag="VNT12_07905"
     CDS             3300..>4304
                     /locus_tag="VNT12_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HWI46082.1"
                     /translation="MNNGLRRYGIWVVVALVGAVCWGMLALARGETVNAGWMLFAALA
                     SYAIAYRFYARFIRDRVLGADNSRATPGERLNNGRDFEPMDRRVLFGHHFAAIAGAGP
                     LVGPVLAAQMGYLPGTIWIIVGVILAGAVQDMTTLFFSMRRNGKSLGQMAREEIGPVG
                     GAAALIAVLSIMVILLAVLALVVVNALAASPWGSFSLAMTIPIALLMGVYLRFLRPGR
                     VLEVSLIGVGLLILAIIGGGWISESSPYAAAWTFTPNQLTFALIVYGFVASVLPVWVL
                     LAPRDYLSTFMKIGTIALLAVGIAITLPPMQMPRFTEFAFNGQGPVFAGSLFPFVFII
                     I"
BASE COUNT          767 a         1318 c         1322 g          897 t
ORIGIN      
        1 ccgcgacaaa cgccgccggg aggcgggagc cctggcatga atgaacgtcc cgccctcctc
       61 ctggaagagg tctacaaatc cttcggggag aacgaggttc tgaaagggat agatctgacg
      121 gtcgagccgc acgaggtggt atgcctgatc ggggcctcgg gctcgggcaa gtccacgctg
      181 ctcaggtgca tcaacctggt cgagccgata gattctggcc gggtcgttgt agatggggag
      241 gagatcaccg cccagggcgt cgacgcgaac cgtatccggc gccgcatagg catcgtctac
      301 cagtccttca acctctttcc acacatgacg gtcctgcgca acatcaccct cgccccgcgt
      361 gaggtcttgg ggacggatcg cgccgaggcc gagaagaatg cccgtgccct actagaacgt
      421 ttcgggctcc tggacaagca agacgagtac cctgacagac tctccggcgg ccagcagcag
      481 cgggtcgcca tcgtaagggc gctcgcgatg cgccctcacc taatgctcct cgacgaggtc
      541 accagcgccc tcgaccctga actggtcgcc gaggtcctcg ccgtcatcaa ggagctggca
      601 cgtgaaggga tgaccatgct catcgcgacc cacgagatgg gcttcgcccg cgacatagca
      661 gaccgcgtct gctttctcga ggccggtcga atcctcgaac agggtaagcc cgagaggatc
      721 ttcaccgccc ctgaacaccc ccgcacccgc cagttcctcg gacgcatcat cgaggccggc
      781 aggctttgag cggttcgctt cgcaaaccgc gacttcaggc atcaggattc aggtgttggg
      841 atttttctgt gaacagagat aagacccgga gggcgatgtg tattaccttg atgggtcaac
      901 caatacgcaa ggatttgcag gccctgacgg tatttggtgg tgctatcgta cggggtgcac
      961 gaaatggttc gaaagttgta gcgcggggct cgtgtgagat ggtaccatga ccctggttgt
     1021 tgcacctcga ctaggaggaa agggtccagt ggactagcaa gccaacaacc atctgcggac
     1081 ccctacaggt ccgcggaccg ggtcggtggt cgttcgccat aagagcggct ttccggcccg
     1141 gatatttttg ggcagttcgc gaaccgcccc tacatgtacg tttatttgaa ccggtaccgg
     1201 cggtacatgc tggcgctttc gtctacgtcg gcttcggtga cgtgggtacg gcccctcgaa
     1261 gctaccctgt cctcgatgcg cttgcggata aaacggcgca tcaggaacgg gctgcgcttt
     1321 atcctgcggt cggcctcggg ctcccaggtg atctttctag tctcaggcat gcatcttctc
     1381 ctctgagttt acagagggat tatattccca cgcttctggc tccgtcagct ccagtctggc
     1441 cttgctgccc tgtacggtga tgcgtctgac aactgtcccg cggggagggc gttacaattc
     1501 tcgtgccatg agacccgaaa cagagaatgc tccacccgcc ctcgaggcga agaacataag
     1561 ccgcctctac aagagcagcg gacgtggggt ctctgatgta ggtttgcgcg tggagcgcgg
     1621 tgaggtcttc ggtctcatgg gaccgaacgg ctcgggtaag agtacccttt tgagagttct
     1681 gtctaccgcc accgcgcctg actcgggcag cttcttcgta ggcggggtag acggcctgaa
     1741 agagaaacgg gaggtccggg cccgcatggg tcttatggtc gaccgtccga cgcactacga
     1801 cgacctcacg ggccgggcga acgcgtactt cttcgcccgc gcctacggga tggaggaggc
     1861 caaagccgat cgtgcgctcg cggagctctt cgaatacttc gacctggcgg agtatgcgaa
     1921 tgacaaggtc aagacctatt cgtacgggat gggcaagaag ctcgccatga tcgaggcgtt
     1981 ggcgcacggg ccggaggtgc tgctgttgga cgagccctcg ctcgggctcg actacacctc
     2041 gcagctcgcc taccagagca ggatcaggga cctcgccggc tcaggcgtcg cggttctgct
     2101 cgcgagcaac caggtcgatg aggtcgaggc tctgtgcgac agggcggcct ttctgcaccg
     2161 tggagaggtc gtcgccgagg gaacgccgga ggatctcatc tcccgtgtcg aaggtatcag
     2221 gcgcatcgtg gcgacgttgc gcaatcccgt ggagtacggt cgagtggctg aggtcggcgg
     2281 tgtagtgcgc gtcgttccgg agggacggga tctcatcttc cactgcgagg aacgtccggg
     2341 catagtagcc gacgtcgtgg ccggcatcgt ccgctcgggc ggtgacatcg tgaacctgtc
     2401 tgtacagagg cccagcctgg aggacgtgtt cgtagagctg accggggagg cgctcaggga
     2461 tgaagtttga tctccccgac gcctattgga agaaggacgt cgcgctgttc ttctccagga
     2521 agtttgcgct aatactgaag atgctctacc ccatggccat aatcgtgcct gtcgcggcga
     2581 gcggcatgcc ggcgaagtac gcggccgccg taataggtat cgtcgcggtg atggcaggga
     2641 ccttcggggc cggtgagagc ctgaccatag acttgaacga tggcatcctg gtgcgcgtcg
     2701 ccctgacccc gctctcgccg tacaggatcg ttttcgagat cctcgccgtg aacgccgtcc
     2761 tcgacctgat gcagctcctg ccggcgctct tcatcgtcta cctcttgcac ccggtctctg
     2821 taggttgggt cttcgcggcc accctcgcca tcttcgcgac tctcgttacc gcgaactgca
     2881 tcgggatctt tatagccaac ttcacctcat cgcccgcgga cgttctcctt tacgcggcag
     2941 cgatactgtt tcccttgatc tacctctcag gcttcttccg caaccagcat cctaccggca
     3001 ttcttcagac actccgaggc ttcatcccct tcgcctacac ggacgatgcc ctgaggacgg
     3061 tcttcggcat gagtacgggc acgactgcgc tcgagattct cgtctacccc accataatct
     3121 gcgccgttat agcaggcgcc gtctgcctca ccgccccacg gctactctcc cagcgtatgt
     3181 agcggccgag acacggctga gatctaacga gaggcggcgg gtctccgcat tcgatacgat
     3241 ttttcgctat cataatgcat atgcttcggt agggccttca gggaaaggca ggaggagtta
     3301 tgaacaacgg tttgcgtcgg tacgggatct gggtcgtagt cgccctcgtc ggggcggtct
     3361 gctggggaat gctggccctc gcacgagggg agacggtaaa cgccggatgg atgctctttg
     3421 cggcactggc atcttacgct atcgcctacc gcttctacgc acgctttatc cgggacaggg
     3481 tactgggagc ggacaattcc cgggcgacac cgggagagcg actcaacaac ggtagggact
     3541 tcgagccgat ggaccggagg gtgctcttcg ggcaccactt cgccgcaata gccggggcgg
     3601 ggccgctagt cggacccgtt ttggctgcgc agatgggcta cttgccgggg acgatctgga
     3661 tcatcgtcgg cgtcatactg gccggcgccg tccaggacat gaccacgctc ttcttctcca
     3721 tgcgaagaaa cggcaagtct ctgggccaga tggcgagaga ggagatcgga cccgtaggag
     3781 gggcggccgc gctcatcgcc gtgctctcca tcatggtgat cctgctcgcc gttctcgccc
     3841 tcgtcgtagt caacgcgctc gcggcctccc cgtgggggtc gttctcgctc gcgatgacca
     3901 tccccatagc cctgctcatg ggcgtttatc tgcgcttctt gcgtccggga cgggtcctcg
     3961 aggtctctct gatcggtgta ggtctactga tactcgccat catcggggga ggttggatct
     4021 ccgagagttc cccctatgcc gcagcgtgga cgttcacccc gaaccagctc accttcgccc
     4081 tcatagtata cggcttcgta gcctcggttt tgccggtgtg ggtcctcctg gcaccccgcg
     4141 actacctctc gaccttcatg aagataggga ccatagcact gctcgccgtg ggcatagcca
     4201 taaccttgcc cccgatgcag atgccccgct ttacggagtt cgccttcaac ggacaggggc
     4261 ctgtcttcgc cggctcgctc ttcccgttcg tgttcatcat tatc
//
LOCUS       DATMJA010000331         5231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_873358, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000331 DATMJA010000000
VERSION     DATMJA010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873358"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..1734
                     /locus_tag="VNT12_07910"
     CDS             439..1734
                     /locus_tag="VNT12_07910"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HWI46083.1"
                     /translation="MAQKPAVHIELRGEEWAVVREGNKRATSTHPTQSEAAERGRELA
                     RRDKSEFFLHSQDGQIREHRDYREGQPSADEGIVDTVSKTMGTVTDVVGGTTGVAAQA
                     AGGASQETGTTAAREDDQDDSVADAASSGESSGRGVGDEVRGLTRAGRTEPLGTPEER
                     YAEYGVYDRYGERIGPLSDLFVDENDEPEYVGVETGLPGNRSVLVPAEAITVDDRLRR
                     MVVSHPRSLVETAPSLGHEDEVTPELERRVRVHYGLDTEERAGSGALYTGAAEPTEAR
                     SADPAPDMSGHEEDDVRVRRSEEEIRVQTREREAGEMRVRKRVRAERERLTVPKKHVE
                     VTVERVPVEGAVPEGDEAATTPQIGEEEIVVPVVEEEIVVEKQPVVKEEIRIRKRVVE
                     EVEVVEEDVRREEVEIDDQTGRDTGFNTDPKESTERDDR"
     gene            complement(1751..2146)
                     /locus_tag="VNT12_07915"
     CDS             complement(1751..2146)
                     /locus_tag="VNT12_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46084.1"
                     /translation="MKRDLLKAEEVGRFFRGNLHCHSNKSDGLVDPEEVVGAYREAGY
                     HFICLSDHFEAEYGWQITDTRPLRDESFTTIVGAEFSSAPWEERDAYWVTAAGIPMDF
                     DLAPFRGSYCRVTVVDIAGRRAWSNPIWP"
     gene            complement(2272..2703)
                     /locus_tag="VNT12_07920"
     CDS             complement(2272..2703)
                     /locus_tag="VNT12_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI46085.1"
                     /translation="MTKKVIELFRERKGRSVRVGNTAEASGRATEGVLGREPDKTGCT
                     DDKAGALARMVLDHREGLGLSQREFAKESGISRTILSKVEQGELYPGGKVRRKLAAAM
                     DLTPMDLWWIGPDAEHRRENSRDGFSGPGRGTRHTEQREAS"
     gene            2996..3053
                     /locus_tag="VNT12_07925"
     tRNA            2996..3053
                     /locus_tag="VNT12_07925"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3021..3023,aa:Asn,seq:gtt)
     gene            3258..4739
                     /locus_tag="VNT12_07930"
     CDS             3258..4739
                     /locus_tag="VNT12_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWI46086.1"
                     /translation="MINGKDILALGWPAGKVIGLGLETVRALESRGLSRDEVLAELED
                     VRQEPGAALERESDGPMAELAREWVRIEAAETGASEEELRAEPLPYHAWGEADVDDAA
                     RLQMETALRLPVAAGGALMADAHVGYGLPIGGVLAVREAVIPWAVGLDIACRLRLSVF
                     ELSSHVLGQKKNEFKRALDDETFFGAGVRHDGHSSHEVLDDPAWGATRFLRGLKDTAR
                     VQIGTSGSGNHFVEFGAFTVPEDLGAEKARVLAPLEPGRSYLALLSHSGSRGVGNKIA
                     TRYSKIAEERRSGLEGEAKKLAWLELSSEEGQEYWLSMRLAGRFAAANHAVIHDRLAR
                     RIGAEAAFVTEHHHNFAFTEQWRGEEMIVHRKGATPAGSGVLGIMPGTMADTGYLTLG
                     LGVEDSVNSASHGAGRRMSRTRAFKELDGDAWREDLQEKGITLLGGALDEAPAAYKDV
                     DGVMAAQADLVEAIGEFSPRIVRMDAGSKKNRRSRKSGRRASR"
     gene            complement(4959..>5231)
                     /locus_tag="VNT12_07935"
     CDS             complement(4959..>5231)
                     /locus_tag="VNT12_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWI46087.1"
                     /translation="QMIGMYAENPPQGFAFSDTAFRIFVLMASRRLNSDRFLTSDYNE
                     GVYTKAGIEWVNDTDMSTVVLRHYPNLAPALRGVTNAFWPWSKVSP"
BASE COUNT         1002 a         1524 c         1755 g          950 t
ORIGIN      
        1 caactcgagg gtctcgttcg ggtcgaggcc cctcggaccg agctccccgg cgtattggag
       61 gcgacggaag acggccctga cgtgggcgac gccgtcctcg tcacgaagca ctatggggac
      121 gcggatcaca tcgtctatgg agtaagtctg gagctctatc ctgtctcgcg gcattcaaac
      181 ctcctgtaaa gatgatgcaa gagagtatgc ctcaatctac gccccgggaa cactccatca
      241 ggcaacaggc ttcatgattt gtgtgggtgt gtatctgctt ggtgggcaga ccgaaatgaa
      301 aggttatttt ggaagagcac cggtgcgggg tctggtgtaa ccctatgctc tcctctttaa
      361 tctaacagat gtaaagccgt ggcagccaca tgtcgacgtc gcagcctcgc gaagaccgac
      421 tgaaaaggag tagacgatat ggcgcaaaaa cctgccgtgc acatcgagct tcggggggag
      481 gagtgggctg tggtgcgcga gggaaacaag cgggcgactt caacccatcc tacccagtcg
      541 gaggcagccg agaggggcag ggaactcgcc cgtcgggaca agagtgagtt cttcctccac
      601 tctcaggacg gacagatcag agagcaccgc gattacaggg aagggcaacc ttccgcagac
      661 gaggggatcg tggacacggt gtcgaagacc atgggaacag tcaccgatgt ggttggcggg
      721 acaacaggcg tcgcggcgca ggccgctggc ggtgcgagcc aggagacggg gaccacggca
      781 gcccgggagg atgaccagga cgatagcgtc gctgacgccg cttcgagcgg tgagagttcc
      841 gggcgggggg ttggagatga ggtgcgcggc ttgaccagag ccgggcgcac tgagccgtta
      901 gggacgccag aagaacggta tgcggaatac ggagtctacg accggtacgg agagaggatc
      961 ggcccgctta gcgatctgtt cgtcgacgag aacgacgagc ctgagtatgt gggggtcgag
     1021 acggggctgc ccgggaaccg gtcggtgctc gtccctgcag aggccatcac cgtcgacgac
     1081 cggctgcggc gcatggtggt ctcacacccc cggagcctgg tggagacagc gccgagcctg
     1141 ggccacgaag acgaggttac gcccgagttg gagcgacggg tgcgcgttca ctacgggctc
     1201 gacacagagg agagggctgg ttccggtgca ctctacaccg gcgcagccga gccgacggag
     1261 gccaggagtg ctgacccggc gccagatatg tccggccatg aagaagacga cgtcagagtg
     1321 cggcgctcgg aggaggagat cagggtccag acgcgggagc gcgaggctgg agagatgcgg
     1381 gtgcgcaaac gggtgcgtgc cgagagggag cgcctcaccg tgccgaagaa gcatgtcgag
     1441 gtaaccgtgg agcgtgtacc cgtcgaagga gccgtgccgg aaggggacga agccgccacc
     1501 acgccgcaga ttggggaaga agagatcgtg gttcccgtgg tcgaggaaga gatcgtggtc
     1561 gaaaaacagc ctgtggtaaa agaggagatc cgtatccgca agcgagtcgt cgaggaagtg
     1621 gaggtcgtgg aggaagacgt ccgcagggaa gaggtcgaga tagacgacca gaccggtcgc
     1681 gatacaggat tcaacacgga cccgaaggag agtacagagc gcgacgacag gtagtttcgc
     1741 agggcgaggg tcagggccag atcggattgc tccatgctcg tcttcccgcg atatcgacga
     1801 cggtgacgcg gcagtaggag ccgcggaagg gggcaagatc gaagtccatt ggaatgcctg
     1861 cggccgtgac ccagtaggca tcgcgctcct cccacggggc ggagctgaac tcggcgccta
     1921 ctatcgtggt gaaactttcg tcgcgcaagg gacgggtgtc ggtaatttgc cagccgtact
     1981 cggcctcgaa gtggtcggag aggcagataa agtggtagcc ggcctcgcga taggcgccta
     2041 cgacttcctc cggatcgacc aggccgtcgg atttgttgga gtggcagtgg aggttgccgc
     2101 gaaagaagcg acctacttct tcagccttta gtagatccct cttcacgaca gtattctacg
     2161 tgaccatttt gctgccggat acggcgtgag ctttgtgacg gggtgttctt tggggccatt
     2221 cggcttgcgt ccgcagcaag aaccaaaccc cccgatctct cgggaggtcc tctatgatgc
     2281 ttctctttgt tctgtgtgcc gtgtcccgcg ccctgggccg ctaaagccgt cgcgggagtt
     2341 ctcccgccga tgctcggcgt cagggccgat ccaccagaga tccatgggcg tgagatccat
     2401 ggccgcagcc agcttgcgtc tgaccttgcc acctggataa agctcgccct gctccacctt
     2461 cgatagtata gtgcggctga tacccgactc ttttgcgaac tctcgttgcg agagccccag
     2521 cccttccctg tggtcgagca ccatccttgc caaagccccc gctttgtcgt cggtacagcc
     2581 ggtcttgtct ggctcccgac ccaaaacacc ctctgtggcc cgccccgatg cctccgccgt
     2641 atttcccacg cgcacgctcc ttcctttgcg ttctctgaat aactcgatta ccttcttcgt
     2701 catgctatga tcatccttct cgtggtctgt gccccaggct cccgccgaaa cgccaccccg
     2761 gcaacaccca tattctacag gatcggtcca tcaatggaca actttttgta gaaatttggt
     2821 tctcaaacgg tttataaatg gtccagtttc ggtatactac ctgttgcgcg accgagagga
     2881 acgcgtttgg cggtctttgg gcctggtccc ctgcggcctt tgaaaatcgg atagcccaca
     2941 gattcatgta gtagcccctg gtcgggtctt tcctggcggg agagctttcc ggcgcagttc
     3001 agtgggcaga atatcgccct gttaaggcga gggtcgcggg ttcgagcccg gccggtctcc
     3061 ctggatcggg atcgactccc gtcttacagg gtccgtagtt caggggagac gcgctgcact
     3121 caaactgcga tgcccgtaac gggcgtgaga gttcgaccct ctctggtgcc accacgtttc
     3181 ggcgagcaga agcaacggcg aggttggcgg cggggctatc cggttttctc caccactatc
     3241 gtaagatggg aggcgacatg atcaacggca aagacatact ggcgcttgga tggccggcag
     3301 ggaaggtgat cgggctcgga ctggagaccg tccgcgctct ggaatctcgc ggcctctcca
     3361 gagacgaagt actcgcagag ctggaggacg tcaggcagga gcccggcgcc gcgctggagc
     3421 gcgagtccga cgggcctatg gccgagctcg cccgggagtg ggtgcgcatc gaggcagccg
     3481 agaccggcgc ctctgaggaa gagcttcgcg ccgaacctct gccctaccac gcgtggggcg
     3541 aggccgacgt cgacgatgcc gccaggctcc agatggagac ggccctgaga ttgccggtgg
     3601 ctgccggcgg cgccctgatg gcggacgccc acgttggcta cgggctgccc attggaggtg
     3661 tgctcgctgt gcgcgaggcc gtgatcccct gggccgttgg cctggatatc gcgtgcagac
     3721 ttcgtctgag cgtcttcgag ctctcctccc acgtgctcgg tcagaagaag aacgaattca
     3781 agcgcgccct agacgatgag actttcttcg gcgctggcgt gcgccacgac gggcactctt
     3841 ctcacgaggt tctcgacgac cccgcctggg gagctacccg ctttctgcgg ggcctcaagg
     3901 acacggcgcg tgtgcagata ggaacctctg ggagcggtaa tcacttcgtc gagttcgggg
     3961 ccttcaccgt gcccgaggac ctcggggccg agaaggcgcg ggttctcgcg ccgctcgaac
     4021 caggtcgctc gtacctggcg cttctctccc actcggggag caggggcgtg ggaaacaaga
     4081 tcgccacccg ctactcgaaa atcgccgagg agcgtcgttc tggcctcgaa ggggaggcga
     4141 agaagctggc gtggctggag ctgtcttcgg aggaaggcca ggaatactgg ctctcgatgc
     4201 gcctggctgg ccgcttcgcc gcggcgaacc acgcggtgat ccacgatcgc ctcgcccgca
     4261 ggatcggggc cgaggcagcg ttcgtcaccg agcaccacca caacttcgcc ttcaccgaac
     4321 agtggcgcgg ggaggagatg atcgtccacc gcaagggcgc cacccccgcg gggtctggcg
     4381 tgctggggat catgccaggg acgatggccg acacaggcta cctcacgctg ggcctcggcg
     4441 tcgaagactc ggtcaacagc gcctctcatg gggccggccg gcgcatgagc cgcacgcggg
     4501 ctttcaagga gctcgacggg gatgcctggc gggaggacct tcaggagaag gggatcacac
     4561 ttctcggcgg cgctctcgac gaggcgcccg ccgcctacaa ggatgtcgac ggggtgatgg
     4621 ccgcccaggc cgacctggtg gaggccatcg gagagttctc gcccaggatc gtacgtatgg
     4681 acgccggctc gaagaagaac cggcgctcgc ggaagtcggg gcgtcgggcg agccgctagg
     4741 ccgaggaagg atgaccacaa ggaggcgcta cgctagtcag caggacatca cgcttcggct
     4801 ggcggccttc gcttgtcaac tactcagcga tagaagagaa gtggggatta gaccctcgac
     4861 gctgacgaaa gtcgttcagt cggagatagc aggcgctgcg accttcagtt tgtatttcac
     4921 gtcgtcgttg tgggccattt cgtctctcgt cggttcactc acggactcac cttcgaccag
     4981 ggccagaaag cgttcgtgac gccccgaagc gcaggggcca ggttggggta gtgccttagc
     5041 accactgtgg acatgtcggt gtcgtttacc cactctatcc ctgccttggt gtagaccccc
     5101 tcgttgtagt cgctcgtgag gaagcggtcg ctgttgagcc gccgggatgc catcaacacg
     5161 aagatgcgga acgccgtgtc gctgaacgcg aagccctgcg gcgggttctc cgcgtacata
     5221 ccgatcatct g
//
LOCUS       DATMJA010000332         2797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_281437, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000332 DATMJA010000000
VERSION     DATMJA010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281437"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VNT12_07940"
     CDS             <1..398
                     /locus_tag="VNT12_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI46088.1"
                     /translation="TFDEWTEEDQFRRREEVNAHIAGHVRTRPAEHWFGVFDEVGIWY
                     APVNDYEDVEEDPQVVHNQAILTFEHPRAGTVRVLSHPVRYDGEAPPVRILPPELGAH
                     TEEILSELGYGQGEIENLKAEGVVRAEEH"
     gene            382..831
                     /locus_tag="VNT12_07945"
     CDS             382..831
                     /locus_tag="VNT12_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWI46089.1"
                     /translation="MQRSTDAKKAVDRAIPLEVGDSVTFSKTVGESDVYLFAGITGDF
                     SPNHINKEVMSGTPYKERIAHGVLSIGFASTTSTLMIEKSGGKAVSLGYDRIRFVGPI
                     FIGDTVTVTYTISEIDEENLRTRADIEVANQRGEVCTVAQHILKFLA"
     gene            1010..1720
                     /locus_tag="VNT12_07950"
     CDS             1010..1720
                     /locus_tag="VNT12_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI46090.1"
                     /translation="MLEDKVTLITGASQGLGRSLALAFAREGARVAINARSEESIAPV
                     AEEVEDAGAEVLALAADVSKSADVERMVGETVERFGRIDVLVNNAGLLGPRVRIEEYP
                     EDQWREVIDANLTGPFLVSKAAIPHLPEGGSVINVVSGVSVDGRAGWGAYSVSKFGIE
                     GLTQILAAELAGRGIRVNAVDPGGMRTEMRAAAYPEEDPATRITPEENTAVFLYLASD
                     ESKDVTGERFRAQEFQRD"
     gene            complement(1733..2524)
                     /locus_tag="VNT12_07955"
     CDS             complement(1733..2524)
                     /locus_tag="VNT12_07955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI46091.1"
                     /translation="MHAARLRRASDRTGRADRDIGEGGVGSNAVDDGLLEGQLAYYRA
                     RAGEYDEWFLRRGRHDRGPEWNRRWFLELDRVRQELDRFGPMGRVLELACGTGLWTVE
                     LARHAASMTAVDASPEVLAINRARLQEARPEVPVRYIRADLFDWRPDDAYDVVFFGFW
                     LSHVPPGRFAAFWKLVLAALRPGGRAFFVDSLGPETPAEKRRLGWRPGDHTMLRRLND
                     GREFRIVKIFYDPSGLEARLADMGWRFSVRRTENHLLYGFGESSR"
     gene            complement(2449..>2797)
                     /locus_tag="VNT12_07960"
     CDS             complement(2449..>2797)
                     /locus_tag="VNT12_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWI46092.1"
                     /translation="THAAIHLEGTGFPWDNEANVYIAGHRLGYPNTESWLTFWDLSSV
                     VGGDKIYVTDANGTEYTYNVFKTFVVSPSDTHVTDTLPGKNILTLQSCTLPDYGERLI
                     VQAELTETSEKAA"
BASE COUNT          562 a          828 c          932 g          475 t
ORIGIN      
        1 atacctttga cgagtggacc gaggaggatc agttccgcag aagggaagag gtgaacgccc
       61 acatagcggg acacgtcagg acgagaccgg cggagcactg gtttggggtg ttcgatgagg
      121 tcggcatctg gtacgcgccg gtcaacgact acgaggacgt cgaggaggac ccgcaggtcg
      181 tccacaacca ggcgatattg accttcgagc acccgagggc gggcacggtg cgggtgctct
      241 cccaccctgt ccgctacgac ggcgaggcgc ccccggtgcg catcctgccc cccgaactcg
      301 gagcgcacac ggaggagata ctctccgagc taggctacgg gcaaggggag atcgaaaacc
      361 taaaggcgga aggagttgta cgtgcagagg agcactgacg cgaaaaaggc ggtggatcgg
      421 gccatacccc tggaggtcgg cgactcggtt actttctcca agaccgtcgg cgagtccgac
      481 gtgtacctgt tcgccggcat cacgggcgac ttcagcccga accacatcaa caaagaggtc
      541 atgtcaggga cgccatacaa ggagcgcatc gcccacggcg tgctctccat aggcttcgcc
      601 tcgacgacct cgacgctcat gatcgagaag tcagggggca aggccgtctc tttgggctac
      661 gaccgcatca ggtttgtagg gccgatcttt atcggggata cggtcacggt cacctacact
      721 atctccgaga tcgacgagga gaacctgagg acgcgtgcgg atatcgaggt tgccaaccag
      781 cgcggcgagg tgtgcaccgt ggcccagcac atcctcaagt tcctggcgta gaaaggcaca
      841 ccgtttgacg gaagtgagga gatccgccga cgggatgcgt atgagcgcga ggccgatttt
      901 cgtactaagc tgacaagctg aagtgctgac aagcggaggc ctgagcgtgc tgagatgctg
      961 acaagcgccc gacagggcgc gtgctgacat gctaccgaag ggaaggaaca tgctagaaga
     1021 caaagtcacg ctcatcaccg gcgcgagcca gggtctcggc aggtcgctcg cgctcgcctt
     1081 tgcgagagag ggcgccaggg tggcgatcaa cgcccgcagt gaagagtcta tcgctccggt
     1141 cgccgaagag gtcgaggatg ctggggcgga ggtcctggcc ctcgcggcgg acgtgtcgaa
     1201 aagcgccgac gtcgagagga tggtcggtga gacggtcgag cggttcggga ggatagacgt
     1261 gctcgtcaac aacgccggtc tgcttggtcc cagggtcagg atagaagagt atcccgagga
     1321 ccagtggaga gaggtcatag acgccaacct caccgggcct ttcctggtct cgaaggcggc
     1381 cattccacac ctccccgagg gcggctccgt gatcaacgtg gtaagcggtg taagcgtcga
     1441 tggcagggcc gggtggggcg cctactcggt gagcaagttc gggatagaag ggctcaccca
     1501 gatcctggcc gcagagctcg cggggcgcgg aatccgcgtg aacgctgtcg acccgggcgg
     1561 gatgcgcacg gagatgcgtg ccgccgccta ccccgaggag gaccccgcca cccgcataac
     1621 cccggaagag aacaccgccg ttttcctgta tctggcctca gacgaatcga aagacgtaac
     1681 gggagagcgt ttcagggcgc aggagttcca gagggactag ccgacgcggc gattacctgc
     1741 tggactcgcc gaaaccgtag agcaggtgat tctccgtcct gcgcaccgag aatcgccaac
     1801 ccatatcggc gaggcgcgct tcgagacctg aggggtcgta gaagatcttg acgatcctga
     1861 actcccgccc gtcgttgagt cgtctgagca tggtgtggtc tcctggcctc cacccgagcc
     1921 gtctcttctc tgcgggagtc tccgggccca gagaatctac gaagaaggca cgtccgcctg
     1981 gccgtaacgc cgccaggacg agcttccaga aagcggcgaa gcgccctgga ggcacgtgcg
     2041 agagccagaa gccgaagaac accacgtcgt aggcgtcatc tggtctccag tcgaacaggt
     2101 cggccctgat gtagcgtacg ggcacctcgg gacgggcctc ctgcagcctg gcgcggttta
     2161 tagcgagtac ctctggcgag gcgtcaacgg cggtcatgct ggcggcgtga cgcgcgagct
     2221 ccacggtcca cagaccggtc ccgcacgcga gctcgagcac cctgcccatt ggcccgaacc
     2281 ggtcgagttc ctggcggacc cgatccagct cgagaaacca cctgcgattc cactccggac
     2341 cgcggtcgtg acgtcctctg cggagaaacc actcatcgta ctctccggcg cgtgcccgat
     2401 agtaggctag ctgcccttcg aggagcccat cgtctacggc gttggatcct acgccgcctt
     2461 ctccgatgtc tcggtcagct cggcctgtac gatcagacgc tcgccgtagt cgggcagcgt
     2521 gcaggactgg agggtgagta tgttcttgcc tggcagggta tctgtaacgt gggtatcaga
     2581 agggctaaca acgaaggtct tgaagacgtt gtaggtgtac tcggtaccgt tagcgtcggt
     2641 gacgtagatc ttgtcgccgc cgacgacgct gctcaggtcc cagaaggtca gccagctctc
     2701 ggtgtttggg tagcccagcc tgtggcccgc gatgtagacg ttggcctcgt tgtcccaggg
     2761 aaagccggta ccttcgaggt ggatggcggc atgcgtc
//
LOCUS       DATMJA010000333         3933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_873726, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000333 DATMJA010000000
VERSION     DATMJA010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873726"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VNT12_07965"
     CDS             <1..642
                     /locus_tag="VNT12_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46093.1"
                     /translation="EQLCTYCGAKSTSKSAEEGECEHCGGRTIPKRIHFYGHENVGAA
                     IARGAMHRLAYPKDDVDAVAHLVANHMRPYGYATSKDPWSDAAVRRFIRDTYLARGDR
                     ELANVDMLLRLARADITGSAPRRKRIAEESWRSLRSRVDEVRAEDAVEKLQSPLSGND
                     LMEQFGLGPGRWIKGLKDYLQNEVVEGRLGKDDKERARGLAEAYAREHDIMEE"
     gene            646..1524
                     /locus_tag="VNT12_07970"
     CDS             646..1524
                     /locus_tag="VNT12_07970"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWI46094.1"
                     /translation="MEKQDPLVSTDWLAAHLDDEKVRVVDIRGYVDKTDLGGGRQKAA
                     YLAACEEYDEAHIPGAVYVDWTSDITDPGDPVPAQIAPPERFADLMGSLGIGDETSVV
                     VYDHAGGQFATRLWWALTYYGHDGVFVLDGGWKKWTAEDRPTTAEVPEPEPAFFTPEL
                     RSGWRTDAEGLLATLAGGDALVLDAREEGQYTGAVARGEGRAGRVPGAAHLHADSLLD
                     PESGTFHSDEELARMLKEAGVPEDRDEPVVAYCNGGVAATVPLFALHRLGYKRLTNYD
                     GSWNEWGVREDLPAER"
     gene            1577..2233
                     /locus_tag="VNT12_07975"
     CDS             1577..2233
                     /locus_tag="VNT12_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWI46095.1"
                     /translation="MKKFNVHSSRARNVISWVLSLLMIVAGVALIASFFLAGRLDSTA
                     TNSDNPGGFNVPKLETTNGENTSNTGPSDKTLELTVPAMARIENDEVPYTSGNDEEAL
                     KTHAAIHLEGTGFPWDNEANVYIAGHRLGYPNTESWLTFWDLSNVVGGDKIYVTDANG
                     TEYTYNVFKTFVVSPSNLRVTETLPGKNILTLQSCTLPDYGKRLIVQAQLTDTSEKAA
                     "
     gene            2245..2949
                     /locus_tag="VNT12_07980"
     CDS             2245..2949
                     /locus_tag="VNT12_07980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI46096.1"
                     /translation="MDDRLLEGQLAYYRARAGEYDEWFLRTGRYDRGPEWNRRWFLEL
                     DRVRQELDRFGPAGRVLELACGTGLWTVELARHAAGITAVDASPEVLDINRARLQEAL
                     PEVPVRYVRTDLFDWRPDGAYDVVFFGFWLSHVPPGRFAAFWDLVRAALRPGGRAFFV
                     DSLRTETWAEKESAGRASRDHTTLRRLNDGREFRIVKVFYDPSCLEARLADMGWRFSV
                     RTTEKHLLYGFGESSK"
     gene            complement(2962..3672)
                     /locus_tag="VNT12_07985"
     CDS             complement(2962..3672)
                     /locus_tag="VNT12_07985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI46097.1"
                     /translation="MLEDKVTLITGASQGLGRALALAFAREGARVAINARSEGSIGPV
                     AEEVEDTGAEVLALAADVSKGADVERMVGEALERFGRIDVLVNNAGLLGPRVRIEEYP
                     EDEWREVIDANLTGPFLVSKAAIPHLPEGGSVINVVSGVSVEGRTEWGAYSVSKFGIE
                     GLTQILAAELTGRGIRVNAVDPGGMRTEMRAAAYPEEDPATRITPEENTAVFLYLASD
                     RSKDVTGERFKAQEFQRD"
BASE COUNT          774 a         1278 c         1226 g          655 t
ORIGIN      
        1 gaacaactct gcacctactg cggggcgaag agcacaagca agagtgccga ggagggggag
       61 tgcgagcatt gcggagggcg cactatcccc aagaggatcc acttctacgg ccacgagaac
      121 gttggggccg ccatagcgcg cggggccatg caccgcctcg cctaccccaa ggacgacgta
      181 gacgccgtcg cccacctcgt cgccaaccac atgcgtcctt acgggtacgc cacgagcaag
      241 gacccgtgga gcgacgccgc ggtcaggcgc tttatcaggg acacctacct cgcgcgcggt
      301 gacagagagc ttgcgaacgt tgacatgctc ctcaggctcg ccagggccga catcacgggg
      361 agcgcaccgc gccggaagcg catcgccgag gagtcctggc gcagcctcag gagcagggtt
      421 gacgaggtaa gggccgagga cgccgtcgag aagcttcaga gccccttgag tggcaacgac
      481 ctcatggagc agttcggcct ggggccaggg cgttggatca aaggtctcaa agactacctg
      541 cagaacgagg tggtcgaagg acgtctcggc aaagacgata aagaaagggc ccgcgggctc
      601 gccgaagcct acgcccgcga gcacgacatc atggaggagt agaagatgga gaaacaggac
      661 cctctcgtct ccaccgactg gctcgccgcg catctcgatg acgagaaggt gcgtgtagtg
      721 gatatcaggg gttatgtaga caagacggac ctcggcggag gccgccagaa ggccgcgtac
      781 ctcgccgcct gcgaggagta cgacgaggcc cacatcccag gcgccgtcta cgttgactgg
      841 accagcgaca tcaccgaccc cggcgacccc gtgccggcgc agatcgcacc gccggaacgt
      901 ttcgcggatc tcatgggatc tctcggcatc ggcgacgaga ccagcgtcgt ggtatacgac
      961 cacgcgggtg gccagttcgc cacacgcctc tggtgggccc tcacgtacta cggccacgac
     1021 ggtgtcttcg tcctcgacgg cggctggaag aagtggacgg ccgaggaccg gccgaccacc
     1081 gccgaagtcc ccgaaccgga gccggccttc ttcacccctg agcttcggag cggctggcgg
     1141 acggatgccg aaggcctcct cgccaccctc gctggtggcg acgctctcgt tctcgatgcg
     1201 cgcgaggagg ggcagtatac cggtgccgtg gcgagaggag agggacgggc gggacgtgta
     1261 ccaggggccg cccacctcca cgccgacagc ctccttgatc ccgagagcgg taccttccac
     1321 tccgacgagg agctggcgcg gatgttgaag gaggcaggtg tgcccgagga tagagacgag
     1381 cccgtcgtcg cctactgcaa cggtggggtc gcggccacgg ttccgctctt tgccctgcac
     1441 aggctcggtt acaagcgact gaccaactac gacggctcgt ggaacgagtg gggtgtcagg
     1501 gaggatctgc ccgcagagcg ttgaacggcg cgaccagaga gcagagagcg acttcggtgt
     1561 tagaatctcc ggcggcatga agaagttcaa cgttcacagt tcgagggcaa gaaacgtcat
     1621 ttcctgggtg ttgagcctgc tcatgatagt ggccggcgtc gcgctcatag cctccttctt
     1681 cctggccgga cgcctggaca gtaccgccac taacagtgac aaccctggcg gtttcaacgt
     1741 gcccaagcta gagaccacaa acggcgagaa cacgagcaac accggcccca gcgacaagac
     1801 cctcgagctt accgtccccg cgatggccag gatcgagaac gacgaagtac cctacacgtc
     1861 ggggaacgac gaggaggcgc tgaagacgca cgccgccatc cacctcgaag gcaccggctt
     1921 tccctgggac aacgaggcca acgtctacat cgcgggccac aggctgggct acccaaacac
     1981 cgagagctgg ctgaccttct gggacctgag caacgtcgtc ggcggcgaca agatctacgt
     2041 caccgacgcc aacggtaccg agtacactta caacgtcttc aagacatttg tcgtcagccc
     2101 atctaatctc cgcgttacgg aaaccctgcc gggcaagaac atactcaccc tccagtcctg
     2161 cacgctgcca gactacggca agcgtctgat cgtacaggcc cagctgaccg acacatcgga
     2221 gaaagcggcg taggatccaa cgccgtggac gatagactcc tcgaagggca gctagcctac
     2281 taccgggcac gcgccgggga gtacgacgaa tggtttctcc gcacgggacg ttacgaccgc
     2341 ggtccggagt ggaatcgcag gtggtttctg gagctggatc gggtccgcca ggaactcgac
     2401 cggttcgggc cggcgggcag ggtgctcgag ctcgcgtgcg gcaccggtct gtggacggtg
     2461 gagctcgcgc gtcacgcggc cggcataacc gccgttgacg cctcgccaga ggtactagat
     2521 atcaaccgcg ccaggctgca ggaggccctt cccgaggtgc ccgtccgcta cgtcaggacc
     2581 gacctgttcg actggagacc agacggcgcc tatgacgtgg tgttcttcgg cttctggctc
     2641 tcgcacgtcc ctccagggcg cttcgccgca ttctgggacc tcgtccgggc ggcgttacgg
     2701 ccaggcggac gcgccttctt cgttgactcc ctgcgcaccg aaacatgggc cgagaaggaa
     2761 agcgctgggc gagcctccag ggaccacacg acgctcagac gactcaacga cgggcgggaa
     2821 ttcaggatcg tcaaggtctt ctacgacccc tcatgcctcg aagcgcgcct cgccgacatg
     2881 ggctggcgct tctcggtgcg cacgacggag aaacacctgc tctacggttt cggcgagtcc
     2941 agcaagtaat cgccgcgtcg gttagtcccg ctggaactcc tgcgccttga aacgctctcc
     3001 cgttacgtct ttcgatctgt ctgaggccag ataaaggaaa accgcggtgt tctcttccgg
     3061 ggttatgcgg gtggctgggt cctcttcggg gtaagcggca gcacgcatct ccgtgcgcat
     3121 cccgcctgga tcaacggcgt ttacgcggat cccgcgtccc gtgagctctg cggccaggat
     3181 ctgggtgagc ccctctatcc cgaacttgct taccgaatag gcgccccact cggtccttcc
     3241 ctcgacgctg acaccgctga ccacgttgat cacggagccg ccctcgggga gatgtgggat
     3301 cgccgccttc gagaccagga atggcccggt aaggttggcg tctatgacct ctctccactc
     3361 gtcctcggga tactcttcta tcctgaccct tggacccagc aaaccggcgt tgttgacgag
     3421 cacgtctatc ctcccgaacc gctcgagcgc ctcaccgacc atcctctcga cgtcggcgcc
     3481 cttcgatacg tccgccgcga gggccaagac ctccgcgccc gtatcttcga cctcttcggc
     3541 gaccggaccg atagaccctt cgctgcgggc gttgatcgcc accctggcgc cctccctcgc
     3601 aaaggcgagc gcgagcgccc tgccgaggcc ctggctagcg ccggtgatga gcgtgacttt
     3661 gtcttctagc atgttctttc ccttcggtag catttcagcc agtcagcact tcagcatttc
     3721 agcttaaacc tttatgcgaa ccgttacccg cgataagccc ctcttcgcgt agcaagtgtc
     3781 ctggctgaca agctgaagtg ctgacaagcg gaggcctgag cgtgctgaga tgctgacaag
     3841 cgcccgacag ggcgcgtgct gacatgctac cgaaaggaag gaacatgcta ggagacaaag
     3901 tcacgctcat caccggcgcg agtcagggcc tcg
//
LOCUS       DATMJA010000334         2761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_873793, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000334 DATMJA010000000
VERSION     DATMJA010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873793"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VNT12_07990"
     CDS             complement(<1..184)
                     /locus_tag="VNT12_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWI46098.1"
                     /translation="MKRTEGRICEGAVLVRERDDDRRDASPLVLLGRREAGRAFYPGV
                     WDVLGGHLEPGETTEQA"
     gene            complement(421..705)
                     /locus_tag="VNT12_07995"
     CDS             complement(421..705)
                     /locus_tag="VNT12_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46099.1"
                     /translation="MGSEVEIPDEKIIALGEYATSPFYDEKERAALEYADAVTLSDRD
                     VGDDLFERVREHVGDDALVELTAVIAWENSSSKFNRALRVPSQGLWRHER"
     gene            complement(724..966)
                     /locus_tag="VNT12_08000"
     CDS             complement(724..966)
                     /locus_tag="VNT12_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46100.1"
                     /translation="MTRVEGVVPERTDKRIRTVLAAQAEKWGAPLLNHLIYARRPTIF
                     HGARAMWGGLYASGLLEPTLVALINRRVASLNGCVF"
     gene            complement(1241..1798)
                     /locus_tag="VNT12_08005"
     CDS             complement(1241..1798)
                     /locus_tag="VNT12_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI46101.1"
                     /translation="MGGSFSKVVLLALCFAVLSSLACSPGETHRPEPVAQRESWYHHF
                     HALPWSHELVGYNRDTTELYMRHFYDHWVGNKDSVRQKEFEAIVDTFARPDAIKASFG
                     WYRATYEEETGPSAHAPEDPISIPTVVRWGELDPVKPASWAEGIEETFPNLDFRFVPG
                     AGHFVPFEAPEETVSAIRTTVSMAR"
     gene            complement(1799..1930)
                     /locus_tag="VNT12_08010"
     CDS             complement(1799..1930)
                     /locus_tag="VNT12_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46102.1"
                     /translation="MVLTTGDEPISALVLEVSDSMVRTVRLVANPEKLAGVRAVGTP"
     gene            complement(1949..>2761)
                     /gene="sigJ"
                     /locus_tag="VNT12_08015"
     CDS             complement(1949..>2761)
                     /gene="sigJ"
                     /locus_tag="VNT12_08015"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HWI46103.1"
                     /translation="VPGRGGRGLSERTSGADRPDAARLFNQHRELLISVAYRVLGSVT
                     DAEDAVQEAWLRWSGVDHSQINNPRAFLVRVTTRLAIDRLRRAKARRESYIGPWLPEP
                     ILTRQDISEDAAMAESVSMAMLVVLETLSPLERAVFVLREAFGMPHSEIADVLGRKEE
                     AVRQLARRAREHVRERRSRFEADQSGQRRVTERFLKATSTGDLEGLMAILSPGVTLVA
                     DSGGKALAPRRPVHGAEKVVRFLLAVATEERTARFLESVGSEPSGELRVHLA"
BASE COUNT          475 a          915 c          886 g          485 t
ORIGIN      
        1 gtgcctgctc ggtggtctcc cccggctcga gatgcccgcc gagcacgtcc cacacgcccg
       61 ggtagaatgc tcgtcccgct tcgcgtctgc cgagcaggac aaggggcgaa gcgtcgcgtc
      121 gatcatcgtc gcgttcgcgg accagcacag cgccctcgca gattcgaccc tcagttctct
      181 tcacgcccat ccttagttct taggagaagg cccgatccgg ctggttcgac aggctcgctc
      241 ctgggacgtc gcacacccgc ccgcgcgtga gacatggccc gggcctcggg tggcggtgtt
      301 gagctgccag gctggtcaca gctcacctcg ctaggaccca agtgtcccgc ccacgaacgc
      361 ccgagagaag cccgcccgga aggtgtggat agcaactcga ccaatagctt cgggttgacc
      421 tcaccgttcg tgcctccaca acccctgcga gggcacgcgc agggcgcggt tgaacttgct
      481 cgacgagttc tcccaagcga tgaccgcagt aagctctacc agggcgtcgt cgccgacgtg
      541 ttcgcgcacg cgttcgaaaa ggtcatctcc gacgtccctg tccgagagcg tgacggcgtc
      601 cgcgtactcc agcgctgccc tctccttctc gtcgtagaac gggctcgtcg cgtactcccc
      661 cagggcgatg atcttctcgt ccggtatctc cacttcgctg cccacggcag cgtttaagtc
      721 ctgtcagaaa acgcagccgt tgagcgaggc caccctgcgg ttgatcaggg cgacgagagt
      781 cggctccagg agacccgagg cgtaaagacc gccccacatc gccctggcac cgtggaaaat
      841 ggtcggacgg cgggcgtaga tgaggtggtt gaggagaggc gcgccccatt tctctgcctg
      901 cgctgccagt acggtcctga tccgtttgtc ggtcctttcc ggaaccactc cttctacgcg
      961 cgtcatccaa cttcctcctt ccagacttcg cttgtaagtg caggacataa gacaggagca
     1021 aggatagcgg ttgtgcgcaa acactgccga tcccgtcagg agcgggcggc gggcctgggg
     1081 ccgtgtcatg gccgcatgga cgcacgtaag tcggacgggt gtccggaagg ggaggggcaa
     1141 cttgaggacc ttccgggcaa tctcgtctcc tagacgtgcc gctggaaggc ttcgctcgaa
     1201 gtcgccggcc tgcgagggag ctgaagagga tagagaacgc tcaccgtgcc atcgagacgg
     1261 tcgtgcggat ggccgatacg gtttcctctg gggcttcgaa cgggacgaag tgtcccgcgc
     1321 ccggcacaaa ccggaaatcc aggttgggga acgtctcctc tataccttcc gcccaggacg
     1381 caggcttcac ggggtccagt tctccccacc tcaccacggt ggggatgctt atcgggtctt
     1441 cgggtgcatg ggccgacggg cccgtctctt cttcgtacgt agcccggtac cagccgaagc
     1501 tggcctttat ggcgtcgggc cgggcgaagg tgtcgacgat cgcctcgaat tccttctggc
     1561 gaacgctgtc cttgttgccc acccaatggt catagaagtg gcgcatatac agctcggtcg
     1621 tgtcccggtt gtagccgacg agttcgtgag accacggcag cgcgtggaag tggtggtacc
     1681 aggactcgcg ctgggcgacg ggctccggcc ggtgtgtttc tccgggagag caggcgaggg
     1741 acgatagcac agcgaagcag agggccagaa gcactacctt ggagaacgac ccgcccacct
     1801 acggtgtccc aacagcgcgg acgccggcca gcttctcggg gttggccact aggcggacag
     1861 tccgcaccat actgtccgaa acttcgagga cgagcgccga gatcggctcg tctccggtgg
     1921 tgagcaccac accaggctca ccgttgacct aggccaagtg tacgcgcaac tcgcccgacg
     1981 gctccgatcc gacggactcc aggaaacggg ccgtccgctc ctcggtcgcg acggcgagca
     2041 ggaaccggac taccttctcc gccccgtgga ccgggcgccg gggcgcgagc gccttcccgc
     2101 cactatcggc aacgagtgtc accccgggtg agaggatcgc catcagtcct tcgaggtccc
     2161 ccgtggaggt cgccttaagg aagcgctctg taactcggcg ctgcccgctc tggtcggcct
     2221 cgaagcgaga ccgccgctcg cgaacgtgct cacgggcgcg cctcgcgagc tgcctgaccg
     2281 cctcctcctt gcgtccgagg acgtctgcga tctctgagtg gggcatcccg aaggcctccc
     2341 tgagaacaaa caccgcccgc tccaaaggtg agagcgtctc cagtaccacg agcatggcca
     2401 tagaaaccga ctcggccatc gccgcatcct ccgagatatc ctgccttgtg aggatgggct
     2461 ctggcagcca ggggccgatg taggactcgc ggcgggcctt ggcgcggcgc aagcgatcta
     2521 tggccagccg ggtagtgacg cgcacgagga aggcgcgggg gttgttgatc tgggagtggt
     2581 ccacccccga ccagcgcagc caggcctcct gcacggcgtc ctcggcgtct gtgacgctgc
     2641 cgaggacgcg gtaggccacg gagatcaaga gctcgcggtg ctggttgaag aggcgggccg
     2701 cgtccggccg atccgcgcct gaagtacgtt cgctcagccc tctacctcct cgtcctggca
     2761 c
//
LOCUS       DATMJA010000335         4880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_222709, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000335 DATMJA010000000
VERSION     DATMJA010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222709"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VNT12_08020"
     CDS             <1..576
                     /locus_tag="VNT12_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46104.1"
                     /translation="VLVSAMMMFGVLFALYAIFDVIVRAFGYGGDRLEAVGNARLALG
                     RMEREIRAAYPYDLTSDPPRRYLFLDPTDPTRPAVPTATRITFGNETGGDRRIDGSEV
                     IGYYLSGHDLKRSKGGSAQTLVDSVPTDGLRFTPCRSVDDCPPALAITDEAEITLLRI
                     ELAVEVRRRGQDPTRQTLATDVYLRNRAEQP"
     gene            573..992
                     /locus_tag="VNT12_08025"
     CDS             573..992
                     /locus_tag="VNT12_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46105.1"
                     /translation="MIWRLLPEERGMALGLAIIVVVIVGVMGAGLLTFVATDLEAVVA
                     VNRGEQAFELAEAGIEVAKVHLANDSSPADWSSGELHMAGMVEGSVSVTVEYDAESGT
                     FKAVSTGQYRETSRKIEATFSIEEGKPKLLSWRELYE"
     gene            1053..1310
                     /locus_tag="VNT12_08030"
     CDS             1053..1310
                     /locus_tag="VNT12_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46106.1"
                     /translation="MDSKTRTRVGGPEDPREGLEAVVALRRTLEALEAAQVENAFVAG
                     WSWARIAEVLGVSKQAVHKKHARRIRERYPGRPPGRKGRGR"
     gene            1307..1816
                     /locus_tag="VNT12_08035"
     CDS             1307..1816
                     /locus_tag="VNT12_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWI46107.1"
                     /translation="MMFTRFTDRSRTSVEAAFDEARMLGHDSLGDEDLLLGILRADEG
                     IAAEALSSLGVTLEGAREESEEMLFGALASIGISLEEVRREAGDAFELRIPDKRRIPI
                     SPWAKKALVEARKEMRRLGDKYLGTEHILLGLLRNADGMAFRMLARMGVSPEMVEERL
                     FELRGRAAG"
     gene            1987..2421
                     /locus_tag="VNT12_08040"
     CDS             1987..2421
                     /locus_tag="VNT12_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46108.1"
                     /translation="MEEREERGGAVRVGMIWGGIGGVVGLLVSLLGSLVGIMVAGFIG
                     FSCGRRAAVAGNRAGALSGLVGGVVAAPVYVLGSTLGALLAARQIGTAGIATTLSDML
                     GKQISADEAWIYFLFSLILSAILEAAILILVSAAAGAWAKRE"
     gene            complement(2446..2922)
                     /locus_tag="VNT12_08045"
     CDS             complement(2446..2922)
                     /locus_tag="VNT12_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWI46109.1"
                     /translation="MDRQLQIQILLDHYEKPRHRGALEDADVAMPGGNPGCGDVVTVY
                     LKAADDQKHIERATYEGEGCTISMAASSMLLEDVQRGDLTMDQVLELDYHEMIEQLGR
                     QVVASRPRCATLGLGTLKAAIRKYQKDRRLERAGVTRPDDGEFVFGEHAAEAARKQ"
     gene            3026..4108
                     /gene="ychF"
                     /locus_tag="VNT12_08050"
     CDS             3026..4108
                     /gene="ychF"
                     /locus_tag="VNT12_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWI46110.1"
                     /translation="MKVGIVGLPNVGKSTVFNSLTRAGAEAQNYPFTTIDPNLGVAAV
                     PDERLVAVAGVIGSKRVVPATVDFVDIAGLVRGASIGEGLGNQFLGHIRECDAIAHVV
                     RCFEDENVVHVHAGVDPSYDIETIDTELLLADLASVERRLERVGRAAKGGDARRQTEA
                     SELERLRDHLSDGSPARVYPATEALAEAMSTLITAKPTLYVANVDEDSVAEGNRYSSQ
                     VERHSSSEGAEAVRICAKLEAEVAELPEDEAAEYLAMLGVETTGFEEFVRAAYRLLGL
                     VTFFTAKEKESRAWTVREGSTVRQAAGRIHTDMERGFIGAEVGDWQDIVAAGSWARAK
                     VEAKVRLEGRDYVVRDGDTVLVRFNV"
     gene            4120..4515
                     /locus_tag="VNT12_08055"
     CDS             4120..4515
                     /locus_tag="VNT12_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HWI46111.1"
                     /translation="MQHFVRPKKPLDRSHLALVTTGGVHLPDQPRFDIDDPAGDCSYR
                     EIPTSANELTWTHAYYRPDEGSDLDAVFPLETLRRLVREGIVGGLSARHFSFMGAIHD
                     PTLLVESTAPEVAGKLVEDQVDVVLLTPS"
BASE COUNT          951 a         1464 c         1571 g          894 t
ORIGIN      
        1 gtactcgtct ccgcgatgat gatgttcggc gtgctcttcg ccctgtatgc gatcttcgac
       61 gtgatcgtga gggccttcgg ttacggcggc gacaggctgg aggccgtcgg gaatgccagg
      121 ctcgcccttg gcaggatgga acgtgagatc agggccgcct atccgtacga tctgacatcg
      181 gaccccccca ggagatacct gttcctggac cccacggatc ccacaaggcc cgcagtacct
      241 acggcaaccc gcatcacctt cggcaacgag accggaggag accgccgtat cgacggctcc
      301 gaagtcatag gctattatct cagcggccac gatctgaaac ggtccaaggg cggcagcgcc
      361 cagacgctcg ttgattcggt tcccaccgac ggactgcgct ttaccccgtg caggagtgtg
      421 gacgattgtc ctcctgctct cgctatcact gatgaggcag agatcacact cctccggata
      481 gagctggccg tcgaagtcag gcgccggggt caagacccaa ccaggcagac gctcgcgacc
      541 gatgtctacc tcagaaaccg cgcagaacaa ccatgatctg gcgcctcctg cccgaggaaa
      601 ggggtatggc actagggctc gcgatcatcg tcgtggtgat cgtcggcgtc atgggagccg
      661 gccttctgac cttcgtcgcc acggacctgg aggccgtcgt ggcagtcaac aggggagagc
      721 aagccttcga gctggcagaa gcagggatcg aggtcgccaa agtacacctc gcgaacgact
      781 cgagcccggc cgactggtcc tcgggcgagc tccacatggc cggcatggtt gaaggctccg
      841 tctccgttac cgtcgaatac gacgccgaaa gcgggacctt caaggcagtc tccacaggcc
      901 agtaccggga gacaagccgt aagatcgaag ctaccttctc catcgaagaa ggcaagccaa
      961 agctcctgag ctggcgagaa ctctacgagt gagataggga aaaatcgtca ataaaggttg
     1021 acagacactg tgtcgtcaac tatgattgac gcatggatag caagacgagg acacgggttg
     1081 gtgggcctga ggaccctcga gagggactcg aagcggtcgt tgcgctgagg cgtacgctgg
     1141 aggctttgga ggcggcgcag gtggagaatg ctttcgtcgc gggatggtcc tgggcgagga
     1201 tcgccgaggt cttgggggtt agcaagcagg ctgtgcacaa gaagcacgcg aggaggatca
     1261 gggagaggta ccctggaaga ccgccaggga gaaagggtcg gggcagatga tgttcacccg
     1321 cttcaccgat agatcccgca cgagcgtaga ggctgccttc gatgaggcca ggatgcttgg
     1381 tcacgactcg ctcggtgacg aggacctctt gcttggcata ctgcgcgcag atgagggtat
     1441 agccgccgaa gcgctctcct cactcggggt gacgctcgaa ggtgcgcgag aggagtcaga
     1501 ggagatgctt tttggtgccc tcgcttcgat agggatctcc ctcgaagagg tgcgcaggga
     1561 ggctggtgat gccttcgagt tacgcatccc ggacaaaagg cgcatcccga tctccccctg
     1621 ggccaagaag gcattggtcg aagcgcgtaa agagatgcga aggttgggag acaaatacct
     1681 cggcaccgag cacatcctgc ttggattact acgcaacgca gatggtatgg ccttcaggat
     1741 gctggccagg atgggcgtct cgcctgagat ggtggaggag cggctcttcg agttgcgcgg
     1801 ccgggcggcc ggctgagaca actcgctgtg gccacagcgt gtcagtagga cggctgtgcc
     1861 gccggttgtc cggctcatgc agagctgaca tgctgaagtg ctgtcaagcc gcctggcgaa
     1921 gccgggcggc gtgctgacat gctgacatgg gcgaccagag gccgcccatg tcgctagaat
     1981 gctggcatgg aggagagaga agaacgcggt ggagccgtca gggtcggaat gatctggggc
     2041 ggcatcggcg gggtggttgg cctgctggtc tcgttgctcg gttcactggt cgggatcatg
     2101 gtcgccggct ttatcggctt ctcctgcggg agacgggctg ccgtggcggg aaatcgggct
     2161 ggcgcgctct ccgggctggt cggtggggtc gtcgccgcgc ccgtgtacgt gctcgggtcc
     2221 acactcgggg cgctcctggc tgcccgccag atcgggaccg ccgggatcgc cacgaccctc
     2281 tcggatatgc tcggtaagca gatctcagcc gatgaggcct ggatctactt cctgttcagc
     2341 ctcattctct ctgccatcct cgaggccgct atcctgatcc tggtctcagc ggccgccgga
     2401 gcctgggcga aaagggagta ggaagagcct cccggcgccc acggctcatt gctttctggc
     2461 cgcctccgcc gcgtgctccc cgaaaacgaa ctccccgtcg tcgggccgcg tcacgccggc
     2521 cctctccagg cggcggtcct tctggtactt cctgatagcg gccttcaggg tccccagacc
     2581 cagggtcgcg cacctggggc gagaggcgac gacctggcgt cctagctgtt cgatcatctc
     2641 gtggtagtcc agctccagta cctgatccat ggtcaggtcg cccctttgaa catcctcgag
     2701 aagcatgctg ctagccgcca tgctgatggt acagccctca ccctcgtagg tcgctcgctc
     2761 tatgtgcttc tggtcgtcgg ccgccttgag gtagaccgta accacgtctc cgcagccggg
     2821 attgccgcca ggcatggcca cgtcggcgtc ctccagggcg ccccgatgcc gcggcttctc
     2881 gtagtgatcc agcagtatct gtatctgtaa ctgcctgtcc aacacgtaac cttccacttg
     2941 ttatcccata gattttctaa cgatgattct atcgcaagct cgtccgtcgt cacgtaaact
     3001 tgctacagtt tgatacactt acttcatgaa agtaggtata gtcgggctgc cgaacgtcgg
     3061 caagtccacg gtattcaata gccttacccg ggcaggggcc gaggcccaga attacccttt
     3121 cacgaccatc gatccgaacc tcggtgtcgc cgctgtcccg gacgaacgcc tggtcgccgt
     3181 ggccggggtg atcggcagca aacgcgtcgt ccccgcgacg gtcgacttcg tggacatcgc
     3241 aggactggtg cggggcgcga gcattgggga ggggctggga aaccagttcc ttggccacat
     3301 cagggaatgc gacgccattg cgcatgtcgt ccgctgcttt gaggacgaga acgtcgtaca
     3361 cgtccacgcc ggtgtagatc ccagttacga catcgagacc atcgacaccg agctgttgct
     3421 cgcagacctc gcgagcgtcg agcggaggct ggaaagggtc gggcgggctg ccaagggtgg
     3481 cgatgccaga cgccagaccg aggcgtccga gttggaacgg ctacgcgacc atctctctga
     3541 cggaagtcct gcgcgggtct acccggcgac ggaggcactg gccgaggcca tgagcaccct
     3601 cataacggcc aagccgacgc tctacgtggc taacgtggat gaggattctg tggcagaagg
     3661 taaccgctac agctcgcagg tcgagaggca cagcagtagc gagggtgctg aggccgtgag
     3721 gatctgcgcg aagctcgaag ccgaggtcgc cgagctgccg gaagacgagg ccgctgagta
     3781 cctggcgatg ctcggggtag agacgacggg tttcgaggag ttcgtgcggg cggcgtaccg
     3841 gctgctcggg ctcgtcacgt tctttaccgc gaaggagaag gagagccggg cctggaccgt
     3901 acgagagggc tcgacggtgc ggcaggctgc cgggcgcatc cacaccgaca tggagcgcgg
     3961 ctttatcggg gccgaggtcg gagactggca ggatatagtc gccgccggct cctgggccag
     4021 ggccaaagtg gaggcgaagg tccgtctcga aggccgcgac tacgtggtaa gagacggcga
     4081 cacggtactc gtccgcttca acgtgtaatg gcatggccgg tgcagcattt cgtcagaccg
     4141 aagaagcccc tcgataggtc acaccttgcg ctcgtcacca caggtggcgt tcacctgccc
     4201 gaccaaccgc gcttcgatat cgacgaccct gccggtgatt gctcctaccg tgagatacca
     4261 acgagtgcca acgagctcac ctggacccac gcctactaca ggccggacga gggcagcgac
     4321 ctggacgccg tctttcccct cgagaccctc cgtagactgg taagagaagg tatagtaggc
     4381 gggctatcgg cacgtcactt ctcctttatg ggagccatcc acgacccgac cctgctggta
     4441 gaaagcacgg cccccgaagt cgccggcaaa ctggtagaag accaggtaga cgtcgtgctg
     4501 ctcacgcctt cgtgacccct gtgccaccgg tccgtcgggc tggtctgcaa cgcgatagaa
     4561 gaacggggca tccccaccgt tacccttgct atggagcacg gcgtcaacgc gccgcgggtc
     4621 ggcttcgtac cctttccata caacctcccg ttaggcaaac cccatgatcg cgaaggacac
     4681 aagcgcatag cgctcgccgc tctcaccctg ctctacgaac tagagcagcc cggcgaagtc
     4741 agcatttcac ctagtcagca ttctcgctcg cgagacctgg ctgacaaggc ggcgcagccg
     4801 ccttgctgaa atgctgaaaa acaggaggcg aaagccggat cgtgctgaca tgctgagata
     4861 taatcgacgt acatattgac
//
LOCUS       DATMJA010000336         3285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_696183, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000336 DATMJA010000000
VERSION     DATMJA010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696183"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VNT12_08060"
     CDS             complement(<1..313)
                     /locus_tag="VNT12_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46112.1"
                     /translation="METEARGSVGRPAPGGFQRERLDRALARATDAPLREGNHLELLR
                     NGPDTYEDWLAAISGAERWVHLDNYIFSDDEVGERFARALIRKAEEGISVRVLYDWFG
                     CL"
     gene            complement(418..825)
                     /locus_tag="VNT12_08065"
     CDS             complement(418..825)
                     /locus_tag="VNT12_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWI46113.1"
                     /translation="MQSFYREILLDHFEHPRNRRELEDADIHEHIDNPLCGDVVTVYA
                     KVQDGAFEEVSFTGRGCSISQASASMLTEQLTGKSREEAQEKIEAFLEMMRTEEREEL
                     GELAALKRIVQTPNRIRCATLAWDAMRRALPTL"
     gene            complement(873..2837)
                     /locus_tag="VNT12_08070"
     CDS             complement(873..2837)
                     /locus_tag="VNT12_08070"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HWI46114.1"
                     /translation="MVRTSTLPAILVVVFAVLIAGPLAAPAEAQDKSFELPRAKVVAD
                     VQPDGSVLVTEEITYLFSGSFEGGYREIPLKEGMSVSDVSVSEYGKAYAPGASAELGS
                     SGAPGTYGTANLGDAYRIVWHYRASYEERTFTVRYRLNGLAVAHDDVVDLYWQAWGDE
                     WEEPLGSLEATVVLPGDAGKGEVKVFGHPASVSGKTSLGPDGVSPTLIASDVPPGQFV
                     EMRVVFPRELLSSTGGARVQPGDGLQQIMDDEAAEARSEARQAWLQAWLVRLQPLFGL
                     LLVVVSVGMIAFVYLRYGREPKVDYSELYEREPPTNDPPAVIGAIISQRPSVGTREFT
                     ATLFDLIRRGVLGAQPVSVKKDSLWGEKTMTDLRVDVGSSDPGSIEDFERSVLTLEDF
                     ERRVLNVAERVLSHGPVNLTDFEERMKRIYDRQANRSSYESFRDAVKREVERRDLVER
                     SPGRWLGPAALVLGLAGAAWFVLSLLGVNVGALISLIFSSFIGLVGAVLVVWVVFRRV
                     FGRADSALGSALHRIWVRRTPKGALLHARWQAFRRYLNDFSRVEDSPPASLALWEQFL
                     VYGIALGVAEQVLEAARLHAPPEVAEGGSFYSPGYDGSVSGPDGFSFSNLENGFSSAF
                     TPPSSSGSGSGGGFSGGGGGGGGGGGGGAW"
     gene            complement(2831..>3285)
                     /locus_tag="VNT12_08075"
     CDS             complement(2831..>3285)
                     /locus_tag="VNT12_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWI46115.1"
                     /translation="NAWSQVDVQLSRRYDLIPNLVATVKAYASHESETFGAVVRARDG
                     ARSAATIEEQGKAESALTEAIGRLLAVAEAYPELRSEERFAELQEELARTEDKISVSR
                     HIYNDTTLNYNDAVSTIPSSLVARLAGFSGRDYFQAEERSRHTPEVKW"
BASE COUNT          565 a         1085 c         1058 g          577 t
ORIGIN      
        1 cgaggcagcc gaaccagtcg tagagcacac ggacgctaat gccctcctca gccttccgga
       61 tcaaagccct cgcgaacctc tccccgacct cgtcgtcgct gaagatgtaa ttgtccaggt
      121 gcacccatcg ctcagcccct gagatggccg cgagccagtc ctcataggtg tcggggccgt
      181 tcctgagaag ctccaagtgg ttgccctccc ggagcggcgc gtcggtggca cgcgccagtg
      241 cgcggtccag cctctcgcgc tggaacccgc ccggcgccgg tcttcccaca gatcctcgcg
      301 cttccgtctc caaccccggc ctcctcgcga agggtatagt cctactctac ccggttgcta
      361 cgctcacgtc gacgaatgat ggtcctgcga cgtggcaatg tacggtaccg gcggtactta
      421 tagagtgggg agagcgcgcc tcatggcatc ccaggcgagg gtggcgcacc tgatgcggtt
      481 gggggtctga acgatacgct tgagggcggc gagctccccg agctcttctc tttcctccgt
      541 gcgcatcatc tcgaggaagg cttcgatctt ctcctgcgcc tcctcgcggc tcttgcccgt
      601 gagttgctcc gtgagcatcg aggccgaagc ctgcgagatg gagcatccgc gtccggtaaa
      661 gctcacctct tcgaaggcgc catcttgcac cttcgcgtag acggtcacca cgtcgccgca
      721 gagaggatta tcgatgtgtt cgtggatgtc ggcatcctcc agctcgcgcc ggttgcgcgg
      781 atgctcgaag tggtcgagca ggatctctct gtagaagctc tgcatcacgt tacagtggat
      841 tgtatcaggg gcgagagagg ggactcctgc ggctaccagg ctcctccgcc accgcctccg
      901 ccacctccgc caccgcctcc gctgaaccct ccgccgctcc ccgagccgga tgaggaggga
      961 ggagtgaagg cgctagagaa gccgttctcg aggttggaga acgagaaacc atcgggtccg
     1021 ctgacggatc cgtcgtagcc cgggctgtag aagctgccgc cctcggcgac ctcgggcggg
     1081 gcgtgcagtc gggcggcttc tagcacctgc tcggctacgc cgagcgcgat gccgtagacc
     1141 agaaactgct cccacagggc gagcgaggca ggcggagagt cctccacgcg gctgaagtcg
     1201 ttgaggtagc ggcggaaggc ttgccagcgg gcgtggagca gggcgccttt cggcgtgcgt
     1261 ctcacccaga tccggtgcag agccgagccg agagccgagt cggcgcggcc gaagacgcgc
     1321 cggaatacca cccagacgac taggaccgcg ccgaccaagc cgatgaagct ggagaagatg
     1381 agggagatca aagccccaac gttcacgccc aagagggaga gtacgaacca cgcggcgcca
     1441 gccagcccaa gcacgagggc cgccggaccc aaccatctac cggggctgcg ctcgacgaga
     1501 tcgcgccgct cgacttcccg cttcaccgcg tccctgaagg actcatatga cgaacggttg
     1561 gcctggcggt cataaatcct tttcatgcgc tcctcgaaat ccgtcaggtt gaccgggcca
     1621 tgggagagca cgcgttcggc cacgttgaga accctgcgct cgaagtcttc gagcgtgaga
     1681 acgctgcgct cgaagtcttc gatggagccg ggatcgctgg agcccacgtc cacgcgaagg
     1741 tcggtcatgg tcttctcgcc ccagaggctg tccttcttta ccgagacggg ctgcgccccc
     1801 aggacgccgc gccggatcag gtcgaacagg gtagcggtga actccctggt tcccaccgag
     1861 ggcctctggc tgatgatcgc gccgattacc gctggcggat cgttcgtggg cggctcccgc
     1921 tcgtagagct cggagtagtc caccttgggc tcccgaccgt agcgcagata gacgaacgct
     1981 atcatcccca ccgacacgac gacgagcagc agcccgaaca gcggctgcag gcgcacgagc
     2041 catgcctgga gccatgcctg gcgggcctca gaacgggcct ccgcggcctc gtcgtccatg
     2101 atctgttgca gcccgtcgcc cggctgcact ctcgccccgc ccgtggaaga gagaagctcc
     2161 ctcgggaaga cgacccgcat ctcgacgaac tgaccgggtg gcacgtcgct tgcgatgaga
     2221 gtgggggaga caccgtccgg gccgaggctc gtcttcccgc tcaccgaagc gggatgtccg
     2281 aagactttga cctcgccctt cccggcatct ccggggagga caacggtcgc ctcgagcgag
     2341 ccaagtggct cttcccactc atcgccccag gcctgccagt agaggtcgac cacgtcgtcg
     2401 tgggccacgg ccagaccatt caggcggtag cggacggtga aagtacgctc ctcgtaggag
     2461 gccctgtagt gccacacgat cctataggcg tcgcccagat tggcggtgcc gtaggtgcca
     2521 ggggcgccgg atgatcctag ttccgctgat gctcctggcg cgtacgcttt accgtactcg
     2581 ctgacggaga cgtcgctcac gctcatcccc tctttcaggg gtatctcgcg gtaccccccc
     2641 tcgaaggagc cactgaagag gtaggtgatt tcctcggtga ctaagaccga gccatcgggt
     2701 tgtacgtcgg caactacctt agccctgggc aactcgaagc tcttgtcctg ggcctcggcc
     2761 ggggcggcga gcgggcccgc tatcaggact gcgaagacga ccaccagtat cgccggcagc
     2821 gtcgaggttc tcaccatttc acctcagggg tgtgcctgga cctctcctcg gcctggaagt
     2881 agtcgcgccc ggagaagccc gccaggcggg cgacgaggct cgaggggatc gtgctaacgg
     2941 cgtcgttgta gttgagggtg gtgtcgttgt agatgtggcg ggagacggag atcttgtcct
     3001 ccgtccgggc gagctcctcc tgcaactctg cgaatctctc ctcggagcgc agctcggggt
     3061 acgcttcggc gacggccagg aggcgcccga tggcctccgt cagggcactc tcggccttgc
     3121 cctgctcctc gatcgtggcc gcggagcggg cgccgtccct ggccctcacg acggctccga
     3181 aagtctccga ctcgtgggag gcataagcct tcacggtcgc gacgaggttg gggataagat
     3241 cgtagcggcg gctgagctgc acgtcgacct gcgaccaggc gttgt
//
LOCUS       DATMJA010000337         4080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_755913, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000337 DATMJA010000000
VERSION     DATMJA010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755913"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1632
                     /gene="radA"
                     /locus_tag="VNT12_08080"
     CDS             250..1632
                     /gene="radA"
                     /locus_tag="VNT12_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWI46116.1"
                     /translation="MATKTLYVCSNCGHAEPKWLGRCPDCGEWSTFVEEVRESKKVVG
                     FAERATRQKAGVAGRTMVLSEVPVIEGGGRMDAGVGELNRVLGGGIVPGSMVLVGGEP
                     GVGKSTLLLQVLGHLGEGCLMVSGEESPRQVALSARRLGVEGRGFKVLSETDVDVIEA
                     TILQERPRAVVVDSIQTLYSPELSGAPGGVGQVRECAARLMRLAKSEGIAVVLVGHVT
                     KEGSIAGPRVLEHMVDTVLQFEGDRFQSFRVLRALKNRFGSTNEVGVFEMTGRGMVEV
                     EDPSAFFLSKREEEVPPGVVTVCLLEGTRPMLVEIESLVAPSPLANPRRIANGVDTGR
                     VNMLCAVLSRRAGLALQDQDVYVNVTGGVRVDEPAADLGVALAIASAMRDRPVGKGTA
                     CFGEVGLTGDVRYVSGAQRRTSEMAKMGFGRIIRPEGTSDGPPDKETRREASVVEVST
                     LVEAVAVALL"
     gene            1629..2714
                     /gene="disA"
                     /locus_tag="VNT12_08085"
     CDS             1629..2714
                     /gene="disA"
                     /locus_tag="VNT12_08085"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HWI46117.1"
                     /translation="MSPQRRHLPAELARALELVAPGTDLREAIDNVMRAHNGALIVLD
                     NPDKLESLGIISGGMKVEVEFAPMRLYELAKMDGAIVVSPDISAIHYANVQLSPDPSL
                     ESEETGMRHLAAHRTAQQTGSLVIAVSERRRVVSLYQGSYGPLVLEEIGVVLSKANSA
                     IATLEKYTRRLREEARALTVHEYDGAVTLREVVGAVGTFEYSARIAEEIEAYVRELGS
                     EGRLVEMQLEQAFHGVPEQYEALLRDYVAEEADYRGVREELRTLSAEQLSDSAEIAHA
                     LGYGPDGQMEELFIKPRGYRQLERVPRLPRRVAERLIREFGSLEEILGATEEELDEVE
                     GVGQARARTIQRGLERQRSLETTGEVS"
     gene            2732..3427
                     /gene="ispD"
                     /locus_tag="VNT12_08090"
     CDS             2732..3427
                     /gene="ispD"
                     /locus_tag="VNT12_08090"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWI46118.1"
                     /translation="MTLVLAGGTGRRMGRPKQFIELLGRPALHYTLQAFEDSPEVARI
                     YAVGDRQRIEALAQAAGISKYTACAEPGEARSFSTRNGLLLCEEDPGTIVLVHDGSRC
                     LVTPDLIKYVVRAANGASEPDGVIPGVPVSDTIKVAREGTVVETLDRTNLWAIQTPQA
                     FRLGLLRRLHDSSEELLRAATDDASLVERDGGRVEVVAGEKTNIKLTSPEDLVFAEAI
                     LAEREGAAREVRP"
     gene            3427..3906
                     /gene="ispF"
                     /locus_tag="VNT12_08095"
     CDS             3427..3906
                     /gene="ispF"
                     /locus_tag="VNT12_08095"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWI46119.1"
                     /translation="MRVGLGVDVHAFAAPEEGRRLVLGGVEIPSDRGLLGHSDADVLA
                     HAITDALLGAAGLEDIGHYFPDTDERYRDADSIELLREVRGLIGGSWGVANVDAVVIC
                     ERPKIRDHREAMRKNLAAALGTEESRVGVRGTTTERLGFPGRGEGIAAQAVCLLERR"
     gene            3885..>4080
                     /locus_tag="VNT12_08100"
     CDS             3885..>4080
                     /locus_tag="VNT12_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWI46120.1"
                     /translation="MPAGTALSGVRVRFAPSPTGLLHLGAARTALFNYLFAGYFGGDL
                     ILRIEDTDRERSRPEFEHAQL"
BASE COUNT          729 a         1222 c         1427 g          702 t
ORIGIN      
        1 gccggggcgc atgtcccggc cttttctttg cccgctctcg aacatttacg tgttcgtcgt
       61 caccggtcta ggaaccgtgg cgatagcggg gttgctgaac gtgccgtggc cccagcctta
      121 gctcccacaa catctccggc gggacaaaca ggcctacaga acccccggtc ttctcaagag
      181 gcgggagtat gacgaacctc tcgacctctt gtttgctgta ctttttcgcc gatccagtac
      241 aatcgaaacg tggctacgaa gactctttat gtttgttcca actgcgggca cgccgaaccg
      301 aagtggctcg ggcggtgccc tgattgcggg gagtggagca ccttcgtcga ggaggtgcgc
      361 gagagcaaga aggtcgtcgg tttcgcggag cgggcgacga ggcagaaggc tggagtcgcg
      421 ggtaggacta tggtccttag cgaggtgccc gtcatcgagg gtgggggcag gatggacgcc
      481 ggggtaggcg agctgaacag ggttctcggg ggtgggatcg tcccagggtc gatggtcctc
      541 gtagggggag aacccggcgt cggcaagagc acccttctgc tccaggtttt gggacacttg
      601 ggggagggct gccttatggt ctcaggcgag gagtcgcccc ggcaggtagc cctgagcgcc
      661 cgtcggctcg gggtcgaggg aaggggcttc aaggtcctct ccgagacgga cgtggatgtt
      721 atcgaggcga ctattctcca ggagcggccg agggctgtgg tcgtggattc gatccagacg
      781 ctctactcac ccgagctctc gggggcgccg ggcggagtcg ggcaggttcg cgagtgcgcg
      841 gcgaggctca tgcggcttgc caagagcgag ggcatcgcgg tggtccttgt gggtcacgtg
      901 acgaaggagg ggtccatcgc aggcccgcgc gtactggagc atatggtcga cacggtgctc
      961 cagttcgagg gggatcggtt ccagagcttc agggtcttgc gggccctcaa gaaccgcttc
     1021 gggtcgacga acgaggtggg ggtcttcgag atgacaggcc gcgggatggt cgaggtcgag
     1081 gacccttctg cgttcttcct ctcgaagagg gaggaagaag tgccgcccgg cgtcgtaacg
     1141 gtctgtcttc tggaggggac gcggccgatg ttggtcgaga tagagagtct cgtcgcgccg
     1201 agcccgctcg ccaacccgcg ccgaatcgcc aacggggtgg acacagggcg ggtcaacatg
     1261 ctctgcgcgg tgttgagccg gagggcaggc ctcgcgctcc aggatcagga cgtttacgtc
     1321 aacgtcaccg ggggtgtgcg ggtcgacgag cctgcggccg acctcggggt ggcgctagcc
     1381 atcgcttcgg cgatgcgcga cagacctgtg ggcaaaggta ctgcctgttt cggcgaggtc
     1441 gggctcactg gggacgtgcg ctacgtctca ggcgcccagc gccggacctc cgagatggcc
     1501 aagatgggct tcggtcgtat aatcaggccg gagggtacgt cagatggccc gccggacaaa
     1561 gagacccgta gagaggcgag cgtcgtggaa gtaagcacct tggtggaagc cgtggcggtg
     1621 gcgttgctat gagtcctcaa cggcggcacc taccggcgga gctggcccgc gccctcgagc
     1681 tcgtggctcc tggtacggat ctcagggagg ccatagataa cgtcatgagg gcacacaacg
     1741 gggccctgat cgtcctcgac aaccccgata agctcgaaag cctcggcatc atctcgggcg
     1801 ggatgaaggt cgaggttgag ttcgcgccga tgcgcctcta cgagctggcg aagatggacg
     1861 gcgcgatcgt ggtctcgccc gatatctccg cgattcacta cgcgaacgta cagcttagtc
     1921 cagacccctc cctcgaatct gaggagaccg gaatgcgcca cctggcggcc caccgcacgg
     1981 cgcagcagac aggcagcctc gtcatcgcgg tctccgagcg taggagggtg gtgagcctct
     2041 accagggctc ctacgggccg ctcgttctgg aggaaatcgg ggtagtgctg tccaaggcga
     2101 actccgcaat cgcgacgctc gagaagtaca ccaggcggct gagggaggag gcgcgggccc
     2161 tgaccgttca cgagtacgac ggggcagtga cgctgcggga ggttgttggc gccgtgggaa
     2221 cattcgagta ttcggccagg atcgccgagg agatagaggc ttacgtcagg gagcttggta
     2281 gcgagggccg gctggtcgag atgcagctcg agcaggcctt ccacggcgtt cccgagcaat
     2341 acgaagcctt gctgagagac tacgtcgccg aagaggcaga ctacaggggc gttagggagg
     2401 agctcaggac tctatcggcc gagcagctct cggactcggc cgagatcgcc catgccctcg
     2461 gctacggccc ggacggccag atggaagagt tgttcatcaa gccccgtgga taccgtcagc
     2521 tagagcgcgt gccacgcctg ccgcgcaggg tcgccgagcg cctcattagg gagttcggct
     2581 ctctggaaga gatcctcggc gcgacggagg aggagctcga cgaggtagag ggggtcggcc
     2641 aggcccgcgc tcgcaccatc caacgcggtc tcgaacgtca gcgcagcctg gagaccactg
     2701 gcgaggtctc atagaagacg ggggatcagc aattacgcta gttctcgccg gcgggacggg
     2761 ccgcaggatg ggccgcccca agcagttcat cgaactcctc ggcaggccgg ccctccacta
     2821 cacgctgcag gccttcgaag attctcccga agtcgcccgt atctacgccg tcggcgacag
     2881 gcagaggatc gaagccctcg cccaagcggc cggcatcagc aagtacaccg cctgcgccga
     2941 gcctggcgaa gcccgctcct tctcgaccag gaacggcttg ctcctctgcg aggaggatcc
     3001 ggggactatc gtgctggtgc acgacgggtc tcgttgcctg gtcacgcccg acctcataaa
     3061 gtatgtcgta agggcggcaa atggagctag cgagcctgac ggtgtcattc caggggttcc
     3121 cgtctctgac accatcaagg tcgccaggga aggcaccgtc gtcgagaccc tggacaggac
     3181 gaacctgtgg gcaatccaga cgccccaggc gtttcgcctc ggcttgctgc gccgtctcca
     3241 tgactcatcc gaagagttgc tccgtgccgc gaccgatgac gcctccctgg tcgagcggga
     3301 tggaggtcgt gtagaggtcg tcgccgggga gaagaccaac atcaagttga cctcccctga
     3361 ggatctggta ttcgccgagg ccattctcgc cgagcgagag ggtgctgccc gtgaggtcag
     3421 gccttgatgc gagtaggact cggtgtcgat gtccacgcct tcgccgcgcc ggaggagggc
     3481 cgcaggctcg tactcggggg ggtcgagatc ccctccgaca gaggcctcct cggccactcc
     3541 gacgccgacg tgctggccca cgccattacg gatgccctcc tcggggcggc gggcctcgaa
     3601 gacatcggcc actacttccc cgacacggac gagcgttaca gagacgcgga ttctatcgag
     3661 ttgctgcgcg aggttcgcgg gctcattggg ggatcgtggg gggttgccaa tgtggatgcc
     3721 gtcgttatct gcgagcggcc aaagatcagg gaccacagag aggccatgcg gaagaacctc
     3781 gccgccgccc tcggaacaga agagtcccgc gtcggtgtac gcggcaccac gaccgagcgt
     3841 ctcggcttcc ctggcagggg cgaaggcata gccgcccagg cagtgtgcct gctggaacgg
     3901 cgttgagcgg ggtcagggtc cgcttcgccc cgagcccgac agggctcctc cacctcggcg
     3961 cggcccgcac cgcgctcttc aattacctct tcgccgggta tttcggggga gacctaatcc
     4021 tccgtatcga ggacaccgac agggagcgga gccgcccgga gttcgaacac gcgcagctca
//
LOCUS       DATMJA010000338         3549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_222976, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000338 DATMJA010000000
VERSION     DATMJA010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222976"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VNT12_08105"
     CDS             complement(<1..852)
                     /locus_tag="VNT12_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWI46121.1"
                     /translation="MTETQAKNFLSGMSRRAVESLGDTVASLPPREATLVKLPDLRPE
                     LSEALRKMGASRLFSHQLEAYERVLAGENVVVATATASGKSLYYKIPAFQNALGRAKS
                     RAVFLYPTKALAQDQLGKIKAFDLRGVHPATYDGDTPHVLRADIRRRANILLTNPDML
                     NIGILPNHEAWADFLRNLEIVAVDEAHVLRGVFGSHVAAVLRRFRRVAMLHGGDPRFV
                     LTSATIANPQELAESLTGLPFTLVDNDGASTGERRVIFRNPPLLDKEKGERQSLLTEG
                     ALVFANLV"
     gene            complement(945..1544)
                     /gene="recR"
                     /locus_tag="VNT12_08110"
     CDS             complement(945..1544)
                     /gene="recR"
                     /locus_tag="VNT12_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWI46122.1"
                     /translation="MATYARPVERLITELSKLPSIGPRSAQRIAFHVIRGKKEDAIGL
                     AEALQEVKEKIKPCRRCFNLTEAEECEICRDTRRDESVICVVEDPYDIGPIERTNEYR
                     GLYHVLGGALSPLDGIEPEDLRIAELLERVRNEGTTELVVATNPNTTGEATALFIAQE
                     VKDLPVRVTALASGLPVGGDLEYADEVTLGRAFAGRREL"
     gene            complement(1552..1884)
                     /locus_tag="VNT12_08115"
     CDS             complement(1552..1884)
                     /locus_tag="VNT12_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWI46123.1"
                     /translation="MSPRQNVNKMMQQVQQMQQQMQQAQEELANETVTASAGGGAVKA
                     TMTGGMELVSIEIDPEVVDPEDVEMLQDMVLAAVNEAMTSSQELANKKLGGITGGLGD
                     LGLNLPGL"
     gene            complement(1918..2160)
                     /locus_tag="VNT12_08120"
     CDS             complement(1918..2160)
                     /locus_tag="VNT12_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46124.1"
                     /translation="MTLEELRSRLEALEETRESARRGLSELASWRERLRALERDKELL
                     LQSYTTRAPEALDSLGPEERRTVYSMLGLRVARQEP"
     gene            complement(2739..3239)
                     /locus_tag="VNT12_08125"
     CDS             complement(2739..3239)
                     /locus_tag="VNT12_08125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI46125.1"
                     /translation="MTPDDASEIGGIVTAPGQAEQKGRNGTWRVLAKLSVSSEPGNER
                     LAMERVAAVVEELGLSEQRLERLKTAVAEATMNAMEHGNRYRPEVPVVIEVLSSGADL
                     SVRINNQGGIRDPAPDKEMPELEAKLEGLQAPRGWGLFLIRNMVDEIYTTSDEVHHTV
                     GLIMHL"
BASE COUNT          607 a         1198 c         1014 g          730 t
ORIGIN      
        1 gacgaggttc gcgaagacca gagcgccctc ggtcagaaga ctctgcctct cccccttctc
       61 cttgtcgagc aagggagggt tgcggaagat gacgcgtcgc tcgcccgtgg aagcgccgtc
      121 gttgtccacg agggtgaaag gcaggccagt gagactctcg gcgagctcct gcgggttggc
      181 tatcgtggcg ctcgtgagta cgaaacgggg atctccaccg tgcaacatcg ccacccgcct
      241 gaaccgcctt agtacggccg ccacgtgcga gccgaagacg ccgcgcagga cgtgcgcctc
      301 gtcgacggcc acgatctcta ggttgcgcag gaagtcggcc caagcctcgt ggttaggcaa
      361 gatgccgatg ttcagcatgt cagggttggt gagcaggatg ttcgccctgc gcctgatgtc
      421 cgcacggagg acgtgtggcg tgtcgccgtc gtaggtggca gggtggacgc cgcgcaggtc
      481 gaaagccttt atcttaccta gctggtcctg ggccagagcc ttcgtcgggt agaggaagac
      541 agcgcggctc ttcgcccggc ccagcgcatt ctggaaggcc gggatcttgt agtacagcga
      601 tttcccgctc gccgtcgccg tcgccacgac gacgttctca ccggccagca cccgctcgta
      661 agcttccaac tggtgcgaga acaacctcga agcgcccatc ttacgcagcg cctcagagag
      721 ctcaggccgg aggtcgggga gctttaccag cgttgcctca cgcggcggaa ggctcgccac
      781 agtatcccca agggactcga ccgcccggcg gctcatcccg ctcagaaagt ttttcgcctg
      841 cgtctcagtc atggatcttc tgcaaagcta tcggcacgaa cccaaaaagt cgatagttga
      901 tagctgacgg agaggtattc ggaagcccac ttcccaaaac cgttctaaag ctcacgtcta
      961 ccggcgaagg cccgtcccag ggtcacctcg tcggcgtact ccagatcccc gccgacgggg
     1021 aggccgctcg cgagggccgt caccctgacc ggcaggtcct tgacctcctg cgctataaac
     1081 agggcggtcg cctctcccgt cgtgttcggg ttggtcgcga cgacgagctc ggttgtgcct
     1141 tcgttgcgga cacgctcgag gagctcggct atccgcaggt cctcgggttc gatcccgtcc
     1201 aacggagaca gggccccgcc gaggacgtgg tagaggcctc ggtactcgtt ggtcctttct
     1261 attggaccta tatcgtaggg gtcttctacg acgcagatca ccgactcgtc ccgacgggtg
     1321 tcacggcaga tctcacactc ctctgcctcg gtcaggttga agcagcgcct gcacggtttg
     1381 atcttctcct tgacctcctg cagcgcctcg gcgagcccta tggcgtcctc cttcttgccc
     1441 ctgataacgt ggaaggcgat gcgctgcgcg ctcctcggcc cgatggaggg tagcttggag
     1501 agctctgtta tgagcctctc tacaggcctt gcgtaggtcg ccaaagaacc cttacagacc
     1561 cggaaggttc aggccgaggt ccccgaggcc gccggtgatc ccaccaagct tcttgttggc
     1621 cagctcctga gagctcgtca tcgcctcgtt gacggccgca agcaccatat cctgcagcat
     1681 ctcgacgtcc tcgggatcta ctacctcagg atctatctcg atggagacca gctccattcc
     1741 gccggtcatc gtcgccttca cggccccacc accggcgctt gcggtaaccg tctcgttcgc
     1801 aagctcctcc tgggcctgct gcatctgctg ctgcatctgc tgaacctgct gcatcatctt
     1861 gttgacattc tgtcgtggtg acaaactaaa ttctcctctc cgaatgcgcc ctgtactcta
     1921 aggctcttgt ctcgctacac gaagtccgag catagagtat accgttcgac gctcctcggg
     1981 tccgaggctg tccagtgcct ctggagcgcg tgtcgtgtag gactggagga gcagttcctt
     2041 gtctcgttcg agtgcccgga ggcgctcgcg ccaacttgcc aactctgaga gcccccgtcg
     2101 ggcgctctca cgcgtttctt cgagggcctc aagcctggac ctcaactctt caagcgtcat
     2161 ggcaccgcgg acagcctccg ccccggacga cctaacaagc tacgctgcct caatctctgc
     2221 cgagcgtacg tcatctgcca gccaatccag gactacggcg gcggtccagg actgctctcc
     2281 agagcctagt gcctcgccgg agaacggttc gaagtactcg gcgaagcccc cttcggcgat
     2341 ctgatcaagg ctggtccggc gtatttgggc cgcgcgctct gcttctccgg ctcggattag
     2401 cgaccaccaa aggagccagt tggcgacggg ccaggcaggg ccgcgccaat agctgcgccg
     2461 gtgaaagctc ggctcttcgg gactagtgct gcctgcgagc gttcgcaggc cgatgttgag
     2521 gtcgcacagc tttgcggccg cgatctgttc ttggttcttg atcagcggta tcgagcgccg
     2581 cagatctctt ccgagaccta gttccgggtc cttcttgaga atctggggcc ctatctctcg
     2641 tagtccctga ggtccgggtg cggctcaagc cggcgacggc cacgccggga tcttggtaga
     2701 cgctcgcctc cagctgttta gccggtatct tcgcctcctt acaggtgcat gatcagcccg
     2761 acagtgtggt ggacttcgtc gctggttgtg tatatctcgt cgaccatgtt cctgatcagg
     2821 aacagacccc agcctcgcgg cgcctgcaat ccttccagct tggcctccaa ctccggcatc
     2881 tccttatcgg gagccgggtc acggatcccg ccctgattgt tgatgcgcac cgacagatcc
     2941 gcgcccgaag agagcacctc gataaccacc ggcacctcgg gccgataccg gttaccatgc
     3001 tccatggcgt tcatcgtcgc ctcggccacg gcggtcttga gcctctcgag gcgctgctcg
     3061 gaaagaccta gctcctccac caccgcggcg actctctcca tcgccaggcg ctcgtttcct
     3121 ggctcactcg atacgcttaa cttcgccagg acgcgccagg taccattgcg tcccttctgc
     3181 tccgcttgtc ctggagccgt cacgatacct ccgatctctg aagcgtcatc aggggtcatg
     3241 tcgtctccca gctcccggac acccccgacg aagcgactcc gtcggacata tgcgtcttgc
     3301 cttctgccac ctcatgagcc taggtcacac ctgccgatcc acccatcgaa acgaaccctt
     3361 cgtgaactac tcatagcctt tcctattcct ctctctgcgc gccagtcgaa ctcctgccaa
     3421 ccgggcgccc agagagtgag cttggcgcgt taaggtgcgc cccttcggcc acccctacgc
     3481 ccagccggac gaccagctct cctccgagga aacctcctag cgaggctaac cccacggcca
     3541 cgaaggaga
//
LOCUS       DATMJA010000339         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_104091, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000339 DATMJA010000000
VERSION     DATMJA010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104091"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VNT12_08130"
     CDS             complement(<1..980)
                     /locus_tag="VNT12_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWI46126.1"
                     /translation="MNLRSQRDLFEIPEDIAYLNCAYMSPQLRPAREAGERAISRKSR
                     PWEITPDLFFEDAEEIRALFARLVGGDAEGVAIVPSVSYGISVAAANVPVREGEKIII
                     LEDQFPSNVYAWRELAERSGARLVTLPRPEDLDWTRALLGEIDADTAVVAVPNCHWTD
                     GSLVDLASVGERVREAGAALVVDAIQSLGAHPFDVSDVRPDFLVAASYKWLLGPYGVG
                     FMYVGEEYREGKPIEHNWINRRGSQDFSGLVVYQDAFQPGARRYDVGERSNFALLPMA
                     AEALRQLLDWGVENVSETIGTLTDLIEQKAEEHGITAIPKERRARHMIGL"
     gene            complement(1010..2419)
                     /locus_tag="VNT12_08135"
     CDS             complement(1010..2419)
                     /locus_tag="VNT12_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI46127.1"
                     /translation="MVVVGGGIGGLAAAALIGRAGHEVTLLEANPWVGGKSRRIELDG
                     RRMDTGPSLLTFPGVWEEFLRHWDTAAGQPGEAAEIADLRLKRLPEVGRYYYRGETAS
                     LPVEEGHPWRAAWERFAGIHGGLGPEVTRLLTADPADRRTLPALGRLLRVYGAKLTTR
                     SYLDSLQWLPEVLREIIAIHTLNAGVAPTLTPALYASMPAIMAVDGVYVPEGGVYEIV
                     LSLYRLARHAGVEVKTGEPVQEIGRDRVVTTEREYKADRVVSDLDPGRLESLLDPERK
                     RAKERLSCSGVAVYATLREELPAGVGVHSVVLPSNPFALYTSLETGKEPRESMAFLNH
                     YRGGEIYPNDGGTLAVLLTAPANGQEYGLEDPFVRREIERVSAAAGLQEPVTRYFGEY
                     EVLDPGYFGAWGSAGGALYGAVRPFWKSGPLHRPLYNDRRRPWLWRVGASVHPGGGIP
                     AVLGGAMISTGRMLRSLVK"
     gene            complement(2425..3267)
                     /locus_tag="VNT12_08140"
     CDS             complement(2425..3267)
                     /locus_tag="VNT12_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWI46128.1"
                     /translation="MILRLFYISRPVLWINTLGPATIGMWLAGDLFAWGALPVLLWLT
                     LPFNLLIYGVNDVFDQETDAKNPRKGTLEGARIDRSEVRPLVFGVIFTNTPFLIYSLL
                     FVPFSATAWMLLYTLLFLGYSIPPARFKARPYLDSLSNAAYAFPLVFVPLALGEGPIW
                     SAALGLMAWSAAKHTFDAVQDVDEDRRTGITTTAVRLGPGGVVLWSGALWVLATVSFA
                     LVSVPVALLNATIAGTLLYVLGQNPTPEMGHWLYRYSIAFPYVAGTFAGVQLVAALSL
                     GAYP"
BASE COUNT          634 a         1149 c         1015 g          592 t
ORIGIN      
        1 atcagcccga tcatgtgccg cgcccgccgc tctttgggga tcgccgtaat accatgttct
       61 tctgccttct gttctatgag gtccgtcaac gtaccaatgg tctccgagac gttctctact
      121 ccccagtcga ggagctggcg cagcgcctcg gccgccatag ggagcagggc gaagttgctc
      181 cgctccccta cgtcgtagcg tctggcgcca ggctggaagg cgtcctggta aactaccaac
      241 cccgagaagt cctggcttcc gcggcggttg atccagttgt gctctatggg cttcccctcc
      301 cgatactcct ccccgacata cataaacccg acaccatatg gtccgagcag ccacttgtac
      361 gatgcggcga cgagaaagtc aggtcgaaca tccgagacgt cgaagggatg tgcccccagc
      421 gactgtatcg cgtcgacgac cagcgcagcc cccgcttcgc ggaccctctc gccgacgctt
      481 gcgaggtcga cgagcgatcc gtcggtccag tggcagttcg ggacggcaac aacggcggtg
      541 tcagcgtcga tctctccgag caaggcacgg gtccagtcga gatcttccgg tcggggtaag
      601 gtgacgagcc tagccccgct ccgctcggcc agctcgcgcc acgcgtagac gttggatggg
      661 aactggtcct cgaggattat tatcttctct ccttctcgca caggcacgtt tgccgcggcg
      721 acggagatcc cgtagctgac ggaagggacg atcgcgacgc cttcggcatc cccgccgacg
      781 agccgcgcga acagcgcccg gatctcttcc gcatcctcga agaacagatc tggtgtgatc
      841 tcccagggcc gtgacttgcg cgatatcgcc ctctccccgg cctcccgcgc cggacgtagc
      901 tgcggcgaca tgtaagcgca gttcagataa gcgatgtcct cgggtatctc gaacaggtca
      961 cgctggcttc tgaggttcat ccctaacgtc ctcccgtccc tttcttgtct cacttaacca
     1021 gagatcggag cattctgccc gtcgagatca tagccccccc gagaacggcc ggtatgccgc
     1081 ctccgggatg caccgaagcc ccgacccgcc acaaccacgg acgtctacga tcgttgtaaa
     1141 gaggcctgtg caacggtccg ctcttccaga aaggacgcac ggccccgtac agcgccccgc
     1201 ccgcgctccc ccaggcgccg aagtaccctg ggtcgaggac ctcatactct ccgaaatatc
     1261 tcgtcaccgg ttcttgcaat ccggccgcag cggagacgcg ttcgatctcc cgcctgacga
     1321 atgggtcctc caaaccgtat tcctgcccgt tcgccggcgc cgtgagcaga acagccagcg
     1381 tccccccgtc gttggggtag atctcacccc cacgatagtg attgaggaac gccatggatt
     1441 cccgcggctc tttgccagtc tcaaggctcg tgtaaagcgc aaaggggttg gaaggtagca
     1501 cgacgctgtg caccccgacc ccggcgggca actcttcccg aagcgtcgcg tacacggcca
     1561 ccccggaaca agacagtctc tccttcgcac gctttctttc ggggtcgagc aagctctcca
     1621 gcctgcccgg gtcgaggtcg cttaccaccc tgtccgcctt gtactctctt tccgtagtca
     1681 cgaccctgtc gcgccctatc tcctgcacag gctcgcctgt cttgacctcg acgcctgcgt
     1741 gtcgggcgag ccggtagaga gagagcacga tctcgtagac gccgccctct ggcacgtaaa
     1801 caccgtcgac ggccatgatc gcaggcatgc tcgcgtatag cgcaggcgtc agcgtcggcg
     1861 ccaccccggc gttgagtgtg tggatggcga tgatctccct caacacctca ggcaaccatt
     1921 gaaggctgtc caggtaggaa cgggttgtca gcttcgcccc gtataccctt agaaggcgtc
     1981 ccagcgcggg aagcgtcctg cggtctgcgg ggtcagccgt cagtagccgc gtgacctcgg
     2041 gcccgagccc gccgtgtatg ccggcgaacc gctcccacgc agcacgccac ggatgacctt
     2101 cctccacagg cagcgacgcc gtctcgccgc ggtagtaata ccgtcctacc tcgggcagcc
     2161 gtttcagcct gagatctgcg atctcggcag cctcgccggg ctgaccggcc gctgtgtccc
     2221 agtgcctcag aaactcctcc cacacaccgg ggaaggtgag gagtgagggc cccgtatcca
     2281 tccgccggcc gtcgagctcg atgcgccggc tcttcccgcc aacccaggga ttggcttcca
     2341 gcagcgtaac ctcatgcccg gcgcgtccga tcagggctgc cgcggccaga cctccgatgc
     2401 cgccgcctac cacgaccacc cggctcaagg gtaagccccc agagacagcg cagcaacgag
     2461 ctgcacgccg gcgaaggtcc cggccacata ggggaaggcg atggagtacc tgtagagcca
     2521 gtgtcccatc tccggcgtgg ggttctggcc caggacatac aacagcgtac ccgctatcgt
     2581 ggcgttcaga agcgcgacgg gcacgctcac gagggcgaag gacacggtgg cgagcaccca
     2641 gagagcaccg ctccagagga cgacgccgcc agggccgagc ctgaccgcgg tcgtcgtgat
     2701 gccggtcctg cgatcctcat caacgtcctg cacagcgtcg aaggtgtgct tggccgcgct
     2761 ccacgccatg agcccgagcg cggcggacca gatgggccct tcgccgaggg cgaggggaac
     2821 gaagacgagc gggaaggcgt aggccgcgtt gctcagtgag tcgaggtatg ggcgggcctt
     2881 gaagcgcgcg ggtgggatgg agtaaccgag gaagagcagc gtgtagagta gcatccaggc
     2941 tgtggcggaa aaaggcacga acaggagaga gtagatcaga aacggcgtat tcgtaaagat
     3001 gacgccgaac accagggggc ggacctcgga gcggtctatc cttgcccctt ccagggtgcc
     3061 tttgcggggg tttttggcgt cggtctcctg gtcgaatacg tcgttgacgc cgtagatgag
     3121 aagattgaaa gggagtgtaa gccacaacag caccggcagc gctccccacg cgaaaaggtc
     3181 cccggcgagc cacatcccga tcgtggccgg acctaaggtg ttgatccaga gcacaggccg
     3241 cgagatgtag aatagacgca ggatcattgc cctaatatat accagcggca cccccgcaga
     3301 ccctttaaca ttgcaagggt gttgacccag ctccgccgca ccacaccgcg acccctgatc
     3361 ctcgcctgcg ccctctccct cgtggcagcc
//
LOCUS       DATMJA010000340         3223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_400376, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000340 DATMJA010000000
VERSION     DATMJA010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400376"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(682..1641)
                     /locus_tag="VNT12_08145"
     CDS             complement(682..1641)
                     /locus_tag="VNT12_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI46129.1"
                     /translation="MIRVRGLRKGYGEVIAIDALNLDVHRGEVLGIFGPVGTGKTTTL
                     EIIEGLRDPDAGDIEVAGYDALRERDKMKRIIGVMPQRVVFPASLTLKDVLRAFANRR
                     GVNVSPESVRGLLNMAALAGEADLRIDEISYGQQRRLWFALALVGDPKILLLDDPTAE
                     LDWRERREFWRVVHSIRDGRMTVIVATRHAEEAGELCDRVALMDRGRVLVCDTPHALI
                     SSLDEEAVVSVGLEGCVDVVRLEPNLNLLHGVLSWDVDSEEISLRTDDAEATVAGLHT
                     LASELGVSLTVPSIRSPGLEDAFVSYAGRRSERAFEPFDCVSA"
     gene            complement(2212..2901)
                     /locus_tag="VNT12_08150"
     CDS             complement(2212..2901)
                     /locus_tag="VNT12_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI46130.1"
                     /translation="MSGGRQGAGWGRPVRVLIVEDQALVRQGMRTLLELEEGLEVVGE
                     AADGFEALERAPDLVPDVALVDVRMPRMNGIELIKRLSEEYPQVRCLILTTFDDDRYV
                     FDGLRAGAKGYLLKNTPSEDLAVAIKKVDRGEAVLGGEITTKVVEELGRLSKAPGEQH
                     PGSESLSEREMEVLSLVAKGLSNREIAKRLYITEGTVKNHISNVLRKLGFRDRTQAAL
                     YAQEHGWIEHP"
     gene            complement(2898..>3223)
                     /locus_tag="VNT12_08155"
     CDS             complement(2898..>3223)
                     /locus_tag="VNT12_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI46131.1"
                     /translation="VQGEERELSPEADLLLYRALQEGLTNALKHSGASRIEARLAFEP
                     SHVRLTVADNGHGTTDSDLGKGLAGFGLLGLRERVAELGGTFKAGGSEGGGFVLTVEL
                     PVRPT"
BASE COUNT          584 a         1052 c          952 g          635 t
ORIGIN      
        1 gaagacgacc gaggagcggg cgaactggtc taggtgcggc gtgtgaatgt aagtgtttcc
       61 gaatgctccc aagtggtcgc gacggaaggt gtccgagacg atgaccacca cgttgggtcg
      121 agtcgacata tgcatgcctc cagcaaaagg taagctcgac gattaccgat gcaagagggg
      181 cggcgcgcgt cggccggctt ggggacgcga gggcgtcata accaggctag gggtttcgcg
      241 gcgggtcgcc atttcgcggg agtgcgttcg cttctcttcg caaaacatcg ctaagccaca
      301 cggcgcaaga gtccgtcggg accacgcccc agggcctcga caaatccatc catgacgttt
      361 cctccctgca acttacgcga tgagcttacg cgtgctaccc tcgggagaca tcgcgctaaa
      421 gtaacgtttc ggaacgagag tgggctccat ctttcggtac atgactttgg ccggctgcgc
      481 agcggcgcga gtatatctcg tgcaagattc tcgcccgtgc ggtcaggtac atgaacctgt
      541 ggtagcgccc atgcagacca cgccgtcgac ccgagtctgc ctgataatga gcgtctgagt
      601 ctgcatgcgg cagacgccga gcctatgttc ccgctggtgg ccgtgacgtt actgaactgc
      661 gcagccgtgg cacgttgccc ctcatgcgga gacacaatca aaaggttcga acgcacgttc
      721 tgatctcctg cccgcgtagg acacaaacgc atcttccagg ccggggctcc ttatggaggg
      781 tacggtaagg ctcacgccta gctcagaagc caaggtatgg agtccggcga ctgtggcctc
      841 ggcgtcgtcg gtacggagac tgatctcctc actgtccaca tcccagctca ggaccccatg
      901 gagcaggttg aggttgggct ccagtctcac cacatccacg catccttcca agccgacgga
      961 gactaccgcc tcctcgtcga gcgagctgat gagagcgtga ggcgtgtcgc agaccaggac
     1021 cctgccacga tccatcaggg cgacccggtc gcacagttct cctgcctcct ccgcgtgccg
     1081 cgttgccacg atcacggtca tccttccgtc ccgtatgctg tgcaccaccc gccagaactc
     1141 ccgtcgctcg cgccagtcga gctctgccgt cgggtcgtca agcaagagaa tcttgggatc
     1201 gcctaccagc gccagcgcga accagagccg ccgctgctgg ccgtatgaga tctcgtctat
     1261 gcggagatcc gcctccccgg cgagagctgc catattcaac agccccctga cactctccgg
     1321 cgagacgttt acgccgcggc ggttcgcaaa ggccctcaga acatccttca gggtcagcga
     1381 ggccgggaag accaccctct ggggcatgac cccgattatc cgtttcattt tgtccctctc
     1441 ccggagagcg tcgtagcccg cgacctcaat atcgccggcg tccggatctc tgagcccctc
     1501 gattatctcc agcgtcgtgg tcttgccggt gccgacggga ccgaagatgc ccaggacctc
     1561 gccgcggtgc acgtctaggt tcagggcgtc gatcgcgatc acctctccgt aacctttgcg
     1621 cagacccctc acgcggatca cggtttcctc tgggcccgtg aaatactttc tctcgctaat
     1681 agtcgtcgcg ggtcccttac gaaccgcccg agccacactc gcctccttga tcggcagatt
     1741 ctctaatgct ctcaaacgct caaggctaga cccgcacgga cggccgcgca tctgcggaaa
     1801 gtcggatcca ggtcaggtta tggattgtgt cctaggtcat gtgcccggcc gcgagcgccg
     1861 ggtccatcga aggacacatg actttcgata gacccgaagt ggctatctga tcctgacttt
     1921 cgtgcgatta tctcggaccg ctgcgagcgt agtctacttg cacggtaacg cgttctagat
     1981 gcagttagta gaaaggaggt tcccagaagg atgcccaata cagaggttcg aatagcgatt
     2041 gagggaacgc tggcagaggc gattactagg cggggagagc atgcaaggcc tggcaaggcg
     2101 gtgcatccga cgcaagacga gaggagcgtc tgggtcgact cttgtctgga gcgtctggag
     2161 cacatctccc catcgtcctg acttgggagg cttcccggac cagcgaggca atcaggggtg
     2221 ctcaatccag ccatgttctt gggcgtagag ggcggcctgg gtgcggtccc tgaagccgag
     2281 tttcctcagg acgtttgaga tgtggttttt gaccgtgcct tctgtgatgt agagcctctt
     2341 cgctatctcg cggttggaga gtcctttggc cacgagggag agtacctcca tctcacgctc
     2401 cgagagtgac tcggacccgg ggtgctgctc gccaggagct ttcgaaagcc gccctagctc
     2461 ctcgacgacc ttcgtggtga tctctccacc gagcaccgcc tcgccccggt ccaccttctt
     2521 tatggcgaca gcgagatctt ccgagggggt gttcttgagc aggtagccct ttgcgcccgc
     2581 gcgcagcccg tcgaagacgt agcggtcgtc gtcgaatgta gtgaggatca ggcaccgcac
     2641 ctgcgggtac tcctcggaga ggcgctttat caactcgatc ccgttcatac gcggcatccg
     2701 cacatccacc agggcgacgt cggggacgag gtcgggcgca cgctcgagcg cctcgaaccc
     2761 gtcggccgcc tccccgacta cctccaggcc ttcttcgagc tctaggagcg ttctcatgcc
     2821 ctggcgcacg agcgcctggt cttccacgat aaggacgcgc acagggcgtc cccacccagc
     2881 gccctgccgc ccgccgctca cgtcggcctt accggcagct ccaccgtcaa cacgaaccct
     2941 cctccttcgc ttccgcccgc cttaaacgtg cctcctagct cggccaccct ctccctcagc
     3001 cccagcaacc cgaagccggc gaggcccttc cccaggtcgc tatcggtcgt cccgtgccca
     3061 ttgtccgcga cggttagcct gacatgtgag ggctcgaagg cgagccgtgc ctcgatccgg
     3121 ctcgcgccgg agtgcttgag agcgttcgtg agcccttcct ggagggctcg gtagaggagc
     3181 agatccgcct caggggagag ttcccgttcc tcgccctgca ccc
//
LOCUS       DATMJA010000341         8729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_756476, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000341 DATMJA010000000
VERSION     DATMJA010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756476"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..509
                     /locus_tag="VNT12_08160"
     CDS             60..509
                     /locus_tag="VNT12_08160"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWI46132.1"
                     /translation="MQRSGENQGVRPPLPPFDEESARQKVQMAQDAWNTRDPEKVSLA
                     YTEDSKWRNRDEFFEGREAIRDFLKRKWARELDYRLEKNLWCFTENRIAVRFEYESHD
                     KDGQWWRSHGNELWEFDENGLMRRRVFLRQVRLPRPPQTTGSAAIGG"
     gene            complement(825..1163)
                     /locus_tag="VNT12_08165"
     CDS             complement(825..1163)
                     /locus_tag="VNT12_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46133.1"
                     /translation="MMDHERTEKELEEIFEEIQHSYKTFADDALALQERTLQFARELL
                     ENPAGRGASGMRDTLENLAERSNGERERFERLARKAGEAYARVLKGPVDEHHHKIEEA
                     EAELKEAGSS"
     gene            complement(1431..2243)
                     /locus_tag="VNT12_08170"
     CDS             complement(1431..2243)
                     /locus_tag="VNT12_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI46134.1"
                     /translation="MVGVSGKERADPARPARITWLGHSTVLVELDGTRVLTDPVLRDR
                     VAHLRRTKPADAGMLYALDGILVSHLHYDHLDFPSLERLGRSVPVVVPSGAGRLLRRR
                     RFEQVVEVETGEEVRIGGLVVRATHAEHEGGRGFLGTEVAALGYLIRGSHQIYFAGDT
                     DLFEGMATLAPDLNIALVPIWGWGPSLGPGHLDPRRAAEALRLLQPLLAVPIHWGTYA
                     PLGFGRLQTAMLTDPVTEFRRHAAELAPEVEVRVLDLGGTLHLDAIPGGYPS"
     gene            complement(2231..4312)
                     /locus_tag="VNT12_08175"
     CDS             complement(2231..4312)
                     /locus_tag="VNT12_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWI46135.1"
                     /translation="MRRLVGRHAVLRVLVVWVITAVTLYVLAAFIPEIVLPGWGSAFA
                     IAAVIGLLNAVVWPLFVRFALPITVLTLGVAALLLNGVIVSIASRIVDGFGVGVLWGI
                     VATFVLTVVNTVVTSLLGIDDDDFYYRNVIQRAAARQGATSSDVPGVVFLEIDGLAHE
                     VVVRALRNGNMPMVGRWMREGSHHLIRWETDWSSQTGACQAGLLHGNSDDWPAFRWWE
                     KEHRRPIVTNHPKDAAELERRHSNGRGLLHEDGASRANILSGDAKHSLLTMSTVLRRD
                     RPGRIGQDYFAYFANPYNITRTVVFALADIAQELYYAAQQRQRDIRPRIKRTLTYALV
                     RSWATVIQRDLQVSAIIGDLYAGRPVAYTTFLAYDEVAHHSGIERPDALSVLRKIDRQ
                     FARIEQAAKEAPRPYHLVVLSDHGQSQGATFLERYGTSLEDVVRKACATDSIHMDEGG
                     SNEAAGYLGASLTEAGSADNVVGRTIRAATKSKRVDGAVQLGEKSREETVRMREAAQE
                     GQMPELSVMASGCLGLISFPREPGRLTLERIECLYPELVPALRDHPGIGFMLVRSERH
                     GAVAIGAHGTHYLDEDRVEGEDPLAPFGPNAARHVRRADGFEHVADIMVNSTYYAEMD
                     EVAAFEELVGSHGGMGGSQSFPFVVLPAGWRLPQQATVGPDNMHRHMRRWLADLGHER
                     YQEASPEAWSG"
     gene            complement(4309..4449)
                     /locus_tag="VNT12_08180"
     CDS             complement(4309..4449)
                     /locus_tag="VNT12_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46136.1"
                     /translation="MDRYPDRQYLLRMLPGNLRERPLDKAEDRTNGIRTLDQCVVGRG
                     DS"
     gene            complement(4704..5090)
                     /locus_tag="VNT12_08185"
     CDS             complement(4704..5090)
                     /locus_tag="VNT12_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46137.1"
                     /translation="MNPTDEHLRRFLVRYGEALSAGDLKAISGCYAVPALVLSDEGSV
                     PISAREEIEAAFDGAAERYRAQGLVGLRPTLVASEALSERLVSVDVRWEYLDEQGRSA
                     QQNGYRYVLRLEDEASPKIQVVIGTP"
     gene            5240..6661
                     /locus_tag="VNT12_08190"
     CDS             5240..6661
                     /locus_tag="VNT12_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWI46138.1"
                     /translation="MGDNRQDTPRLPLAKPLDEAAIQAFSSRVRGQLIRPEDDGYDEA
                     RTVFYGGFDRRPALIVRVKDATDISHVIALARESGLELAIKSGGHSVAGHSTIDGGIV
                     LDITEMKNLELDVEGRTAWAQTGLTAGEYTAAAGAHGLATGFGDTGSVGIGGITLGGG
                     VGYLVRKHGLTIDDLLAAEVVTADGELLRADAKSHPDLFWAIRGGGGNFGVATRFQFR
                     LHEVDEIVGGMLILPATPEVIASFVTEAEAAPEELSTIANVMTAPPMPFLPAEVHGQL
                     VIMAMLVYAGEEVEAGRRAVAPFRALAEPIVDMLKPMPYPQIYPPEEEDFHPTAVART
                     MFIDSIDRDVSQTILEHLRASEAPMRAAQIRVLGGAMARVPAEATAFAHRSSRIMVNV
                     AAFYEGTQERPAREAWVSRFAAALRQGEGGAYVGFLGEEGEERVREAYPESTWDRLAE
                     IKRRYDSTNLFRLNQNIPPAIEQ"
     gene            7156..8274
                     /locus_tag="VNT12_08195"
     CDS             7156..8274
                     /locus_tag="VNT12_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46139.1"
                     /translation="MVSFLAFPIVGALIASRHPKNPVGWICLAIGLFWMLIFLGDSIP
                     GTGPYPVTTDALTQATWIPPVGLLGIYLILLFPDGKLPSRRWRPLAWLSGMVMVLASL
                     GITFAPGPLEEHPGVRNPLGLEALGHPWVATAVFIVLLLLPLCMLASALSLVLRYRRS
                     GGEEREQIKWIAFAASVVGLLYMITMVSSLTFSGVWGAAGTPLWLGILQQAALVSFTT
                     VPIAVGFAVLKYRLYDIDIVINRALVYGALTALLVTVYVGSVVSLQGALRALSGQESQ
                     LAVVASTLLIAAIFNPLRRRIQAFVDRRFYRRKYDARKTLEAFSAKLRDETNLVALSD
                     DLVGMVRETMQPAHVTLWLRPELPPREGEHKSRWSTLR"
     gene            complement(8372..>8729)
                     /locus_tag="VNT12_08200"
     CDS             complement(8372..>8729)
                     /locus_tag="VNT12_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46140.1"
                     /translation="GGVSAVNAFFYPGTIGVLSMLIAYMVTNVGALRFLFLSGRVRAW
                     EAVFPVIALVILVYVIYANVYPVPDFPFNLFPYIVAAWLMVGLGIILLVPGLARRIGA
                     NLAEREGLAVEESPRL"
BASE COUNT         1591 a         2711 c         2775 g         1652 t
ORIGIN      
        1 tctgtgctct acctatcgtg tacgcttatc gtagagaggc ggaaaaggag gaccgaagta
       61 tgcagcggag cggcgagaat cagggagtta gacctccgct accacccttc gacgaggaga
      121 gcgccaggca gaaggtgcag atggcacagg acgcctggaa cacccgcgac cctgagaagg
      181 tctccctcgc ctacaccgag gactcgaagt ggcgcaaccg cgacgagttc ttcgagggcc
      241 gcgaggcaat cagggacttc ttgaagcgca agtgggctcg tgagctcgat taccgactcg
      301 aaaagaacct ctggtgcttc acggagaacc gcatcgcggt caggttcgag tacgagtcac
      361 acgacaagga tgggcaatgg tggcgtagcc acggtaatga gttgtgggag ttcgacgaga
      421 acggcctgat gcgccgccgc gtcttcctca gacaggttcg cctacctcgc cctccacaga
      481 cgacgggatc cgctgcgatc ggcggttgag caggtacgta tcgggaacgc ataaactcgc
      541 gtgtcgagcc gtgggtccct gcacaggagc tctcctacgc gccgtggccg ggtcccggtg
      601 acagcgaggg ctacgactag atggctctga ccgggtttcc cttgcagcgg gacgtgacca
      661 ccccgcgtgt gggcagcatt gtttgccttt ctaccttcat cggatgctgg ttgctcgcga
      721 gcggggcgat gcaactagtt gggcagcgct tggtagtagg gctataggac cccgtaggga
      781 ttccgaagta gggcgaggag gagttacgtc tctcacccga gaggttagga ggaacccgcc
      841 tcctttagtt ccgcctcggc ctcctcaatc ttgtgatgat gctcatcgac ggggcccttg
      901 agcaccctcg cgtaagcttc gccagccttc ctcgccagcc tctcgaaccg ctcgcgctcg
      961 ccattgctcc tttcggccaa gttctccaga gtgtcccgca tgcctgaagc cccccggccc
     1021 gcggggttct ccagcaactc tcgggcgaac tggagggtgc gctcttggag ggcgagggca
     1081 tcgtccgcga atgtcttgta ggagtgctgg atctcctcga agatctcctc gagttccttc
     1141 tccgttctct cgtggtccat catctcttgt gatcactccc ttctcttgtg gaggcgtggc
     1201 ggtagtacag tggaaattct agagaaaaga ctttacagcg caagcggcgg gacgagagat
     1261 gcacgtgccc tgtgcgcctt ggggtacgca tctggctgcg gcgagcccgc tacgagcttg
     1321 ccataggtaa gagacgacgt cttgcgctga ccgtcgagac cggcgcaact ttcgcccccg
     1381 ggtctttagc caactccggt gcaacggtag ccggccactg cttgctcggc ttagctcgga
     1441 tagccaccgg ggatcgcatc gagatggagc gttccgccca gatccagaac acgcacctca
     1501 acctcgggcg cgagctcagc tgcgtggcgg cgaaactccg ttacggggtc agtgagcata
     1561 gccgtctgca aacgtccgaa gccgaggggc gcataggttc cccagtggat cgggacagcg
     1621 aggagcggct gcaggagccg gagcgcctcg gcggcgcggc gcggatcgag gtggccaggg
     1681 ccgagcgaag gaccccaacc ccagatcggt accagcgcga tatttagatc gggtgcaagc
     1741 gtggccatcc cctcgaagag gtcggtgtcc cccgcgaagt agatctggtg cgagccgcgg
     1801 atcaggtaac ccagggctgc cacctccgtt cccagaaacc ctcgtcctcc ttcatgttca
     1861 gcgtgggttg cgcgcacgac gaggcctccg atccgaacct cctcgccagt ctcgacctcg
     1921 acgacctgtt cgaagcgacg cctgcggagc agcctgccgg cgccgctcgg gacgacgacg
     1981 ggcacagacc ggccgaggcg ctcgagcgag ggaaagtcaa ggtgatcgta gtggaggtgg
     2041 gagacgagga tcccgtcgag agcgtagagc atcccagcgt ctgccggttt tgtacgccgg
     2101 aggtgcgcca cgcggtcgcg gagcaccggg tcggtcagta cccgcgtgcc gtctaactcg
     2161 accaggacag tgctgtggcc gagccaggtt atgcgtgcgg ggcgcgccgg gtcggcacgc
     2221 tccttaccac tcaccccgac cacgcctcag gcgatgcctc ctggtaccgc tcgtgaccga
     2281 ggtcggcgag ccagcggcgc atgtgccggt gcatgttatc ggggccgacg gtagcttgtt
     2341 ggggcaggcg ccagcccgcg ggcaggacga cgaacgggaa cgactgggaa ccacccatgc
     2401 cgccgtgaga gccgacgagc tcctcgaagg cggcaacttc atccatctcc gcgtagtagg
     2461 tgctattgac catgatgtcg gcgacgtgct cgaagccgtc cgcgcgcctg acgtgccggg
     2521 cggcgttcgg gccgaagggc gccaaaggat cctccccttc cacccggtcc tcatcaaggt
     2581 aatgcgttcc gtgggcgcct atcgccacag ccccatgccg ctcggagcgc accagcatga
     2641 agccgatgcc ggggtggtcg cgcagcgccg ggacgagctc tgggtagaga cactcgatgc
     2701 gctcgagcgt aagtcgcccc ggctcgcgtg ggaacgagat gagcccgagg caacccgagg
     2761 ccatcaccga cagttcgggc atttggccct cctgcgccgc ttcgcgcatc cttacggttt
     2821 cttcgcgaga tttctcgccg agttgcacag caccgtctac ccgcttgctc ttcgtcgccg
     2881 cgcggatcgt gcggccgaca acgttgtctg cggaccccgc ttcggtcagc gatgcgccaa
     2941 ggtatccggc tgcctcgttg cttcctcctt cgtccatgtg gatgctgtcg gtggcgcacg
     3001 ccttgcgcac gacatcctcc agcgacgtgc catagcgctc gaggaaggtg gcgccctgtg
     3061 actgaccgtg gtcggagagg accacgagat ggtacgggcg tggggcctcc ttcgccgcct
     3121 gctcgatccg cgcgaactgc cgatcgatct tgcgcagcac cgacagcgca tcaggccgct
     3181 cgatgccaga gtggtgggcg acctcatcat aggcaagaaa ggttgtgtag gcgactggcc
     3241 ggccggcgta gaggtcgccg atgatcgccg acacctggag gtcgcgctga atcacagtag
     3301 cccacgagcg cacgagcgcg taggttagcg tgcgcttgat ccgcgggcgg atgtcgcgct
     3361 ggcgctgctg ggcagcatag tagagctcct gagcaatatc ggccaaggcg aacacgacgg
     3421 ttctcgtgat gttgtaaggg ttggcgaagt aagcgaagta gtcctgacca atgcgcccgg
     3481 gtcgatcgcg cctcagcacc gtgctcatag tcagcaggct gtgtttcgcg tcgcccgaga
     3541 ggatattcgc ccggctggcg ccgtcttcgt gcaacaggcc gcgtccgttc gaatgacgac
     3601 gctccagctc ggcggcgtcc ttgggatggt tcgtgacgat cggtcgccta tgctccttct
     3661 cccaccagcg aaacgccggc caatcgtcgc tgttgccgtg cagcagtcct gcctggcatg
     3721 cacccgtctg cgacgaccag tccgtctccc accggatgag atggtgtgat ccttcgcgca
     3781 tccatcggcc caccatcggc atgttgccgt tgcgtagcgc gcgcaccacg acctcgtgcg
     3841 cgaggccgtc gatctcgagg aagaccacgc cggggacgtc ggaagaagta gcaccttggc
     3901 gtgcggcggc tctctggatg acgttgcggt agtagaagtc gtcgtcgtcg attcccagga
     3961 gcgacgtgac gaccgtgttt accaccgtga ggacgaaggt cgctacgatg ccccagagca
     4021 ccccaacccc gaacccgtcg acgatcctcg atgctatcga cacaatgacg ccgttaagca
     4081 gtagcgcggc gaccccaagg gtcaacacgg tgataggcag cgcgaacctg acgaacagcg
     4141 gccacaccac ggcgtttagg agaccgatca cggccgcgat agcaaaagca gagccccagc
     4201 ccggaaggac tatctcgggg atgaaggcgg cgaggacgta gagcgtcacc gccgtgatca
     4261 cccacacgac caacacccgg agcacagcgt ggcggccgac caagcgcctc atgagtctcc
     4321 tcgtccgacc acgcactggt ccaaagtgcg gatgccgttc gtgcgatcct ctgctttatc
     4381 gagcgggcgc tccctcaagt taccagggag catccgcagc agatactgcc tgtcggggta
     4441 gcgatccatc gcaacatcct ttcgaggaga gccggtcttc tgatggccgc cgttttgcaa
     4501 tagcgctatt ttagagtaag cctcagcaag gtggtgagtc atcattcgta gccggatagc
     4561 agcaccccac ttaactgtgg agagtagtca agccgcgtta gcggcctcca acatctgggt
     4621 cgtctcccgt tacggacctg taggtaagcg tgaaattgtc ggaagttcca gagtactaag
     4681 gaattctcgg acgtccgctt agtctaggga gtccctatga cgacctggat cttgggagac
     4741 gcctcgtcct ctagccggag cacgtagcgg tagccgtttt gctgcgcgct gcgaccctgc
     4801 tcgtcgaggt attcccaacg cacgtccacg gagaccagcc tctcgctaag agcctccgag
     4861 gctacaaggg ttggccggag gcccacgagc ccctgcgcgc ggtagcgttc ggccgctccg
     4921 tcgaaggcgg cctctatctc ctcgcgtgcg gagatcggga cgctgccctc gtccgacaac
     4981 actagggcag gaacggcgta gcagccggag atggccttca ggtcccccgc ggacaaagcc
     5041 tccccgtagc ggacgaggaa acgtcgcagg tgttcatccg ttgggttcat cctcgatcct
     5101 cctgctcagt cgctctgacc aatcttacga ccacgctgca aggatctcca tcccctgatc
     5161 cttcttttgg gggatgccac taccccggat ggccagtatg tttcttatct gaagcaaacc
     5221 taggatcgga ggtcagatca tgggagacaa taggcaagat accccgcggt tgccccttgc
     5281 caagcctctc gatgaggcgg ctatccaagc gttttcgtcc agagtccgcg gccagctgat
     5341 acgccccgag gacgacggct atgacgaggc gcgcaccgtc ttctacggcg gcttcgaccg
     5401 ccgcccagcg ctaattgtcc gggttaagga cgctaccgac atctcccacg ttatcgctct
     5461 ggcgcgtgag agtgggctgg agctcgccat caagagcggc ggccacagcg tagccggtca
     5521 cagcaccata gacgggggga tcgtgctcga catcacggag atgaagaact tagagctcga
     5581 cgtcgagggg cgtaccgcgt gggcccagac cggcctcacc gcgggcgagt ataccgctgc
     5641 cgcgggcgcg cacggacttg cgacgggctt tggagacacc ggctcggtcg ggataggggg
     5701 gatcacccta ggcggcggcg tcggctacct ggtgcgtaag cacggcctca ccatagacga
     5761 cctgctggca gccgaggtcg tgactgcgga cggagagctc ctccgcgcgg acgccaagag
     5821 tcatcctgac ctcttctggg cgatccgtgg cggcggaggc aatttcggcg tcgcgacccg
     5881 gttccagttc aggctgcacg aggtcgatga gatcgtcggc ggcatgttga tcctgccggc
     5941 gactcctgag gtcatagcct cgtttgtgac ggaagcggag gctgcaccgg aggagctctc
     6001 gaccatcgcc aacgtgatga cggcaccgcc gatgccgttc ttgccggccg aggtgcatgg
     6061 ccagctcgtc atcatggcga tgctggtgta tgctggcgaa gaggttgagg ccggccggcg
     6121 cgccgtcgcg ccgtttcggg cactcgccga gccgatcgtc gacatgctca agccgatgcc
     6181 ctacccgcag atttaccccc ctgaggagga ggacttccac ccgaccgcgg tagcgcgcac
     6241 gatgttcatc gactccatag accgcgacgt ttcgcagacg atcctcgagc accttcgggc
     6301 ctccgaggca ccgatgcgtg cggcgcagat ccgggtcttg ggtggggcga tggctcgcgt
     6361 acccgcggag gccactgcct tcgcccaccg ctcctcacgc attatggtaa acgtcgccgc
     6421 tttctatgaa ggtacacagg agcggcctgc gcgcgaggcg tgggtaagtc gcttcgcggc
     6481 ggctttgcgt cagggtgagg gtggtgcgta cgtgggcttc ctgggcgagg agggcgagga
     6541 gagggtccgc gaggcctatc ccgagtctac ctgggatagg ctcgctgaga tcaagcgtcg
     6601 ctatgactcc actaacctct tccggctcaa ccagaacatc ccgccggcga tcgagcagta
     6661 ggacgcaaag ccaggaacct cgagctcgac ccgcactgta tcatcgcagc gagcagggac
     6721 gcgctcgatc tggtggtcga gggcgaggcc tcgacgttga cggatgaagc ctggctccat
     6781 cgcgttgccc atttccaagt acggttggcc agtgaccgtt cgcgacggcg ccgtttacgc
     6841 tgacggtgcg ccgaccgcgg gtccgccccc gtacatcacc gagggtgggc aaaatcgatc
     6901 catatgttgc tgcctcgtaa cgcttctcgt aacatgttgg aggtgttagt gctgtagagt
     6961 gatgctgtga cacaaggggc aagggaacaa ggtcgaggtg tcgctaagtt gaggacccgc
     7021 gcctccgcat ggctagcctg gtcgctagcc ggtctctccg tgggcatatt cctagccagc
     7081 gtcgtactct atgtcctcgt acgttcatcg caggagggtc cgagcactag cggcgctctc
     7141 agcgagctgt tgatcttggt ttctttccta gccttcccca tagtgggtgc cctgatcgcc
     7201 tctaggcacc ccaagaaccc agtaggctgg atctgcctgg ccatcggtct gttctggatg
     7261 ctcatctttc taggggactc catccctggc accggacctt accccgtgac gaccgacgcg
     7321 ctgacccagg cgacatggat accccccgtt gggctgctcg gcatctacct tatcctgctc
     7381 ttcccagacg gtaagctacc ttcgaggaga tggcgtccgc tggcctggct ctccgggatg
     7441 gtgatggtgt tggccagctt gggaatcacc ttcgcccccg ggcctctgga agaacatccg
     7501 ggggtgcgca acccactcgg gcttgaggcg ctagggcacc cttgggtggc gactgccgtg
     7561 tttattgtcc tcctgctgct ccccttgtgc atgctcgcct cggctttgag cctggtgttg
     7621 cgctaccggc gttctggggg tgaggagcga gagcagatca agtggatagc ctttgctgct
     7681 tcggtggtgg gcctgcttta catgatcacc atggttagct cgttaacctt ctccggggtc
     7741 tggggtgcgg caggcacgcc gttgtggcta ggtatcctac agcaagcggc gttagtgagc
     7801 ttcacgacag tcccgatcgc tgtgggcttc gctgtcctca agtaccgcct ctacgacata
     7861 gacatcgtca taaaccgcgc actggtctac ggcgctttga cggcgctgct ggtaacggtg
     7921 tacgtgggaa gcgtggtgtc gttgcaggga gcattgcggg cgttgagcgg gcaagagtct
     7981 cagttagcgg ttgtagcatc tacgctgctt atcgcagcga tcttcaaccc cctgagacgc
     8041 cgtatccagg cgttcgtgga tcgtcgcttc taccgccgta agtacgatgc tagaaagacc
     8101 ctagaagcct tctccgccaa gctcagggac gagacaaact tagtcgctct gagcgacgac
     8161 ctggtgggta tggtaaggga gacgatgcaa ccagcccatg ttactttgtg gttgagaccc
     8221 gagctacctc cgagggaagg cgaacacaag agtaggtggt ccacccttcg ctgagggggc
     8281 cggcacggag ttattgtgac ggctaacgac aagcctctcc acgaggtacc tacggaggtc
     8341 cttcgcgcag cgctccaggg ttgccgcagg gctataatct agggctctct tcgaccgcga
     8401 gtccctcccg ctctgcaagg ttcgcgccga tacgccgcgc cagtccaggc accagcagga
     8461 tgatccccag tcccaccatc aaccatgctg ccacgatgta agggaagagg ttgaatggga
     8521 agtccggcac cgggtagacg tttgcgtaga tcacgtagac caggatcacg agcgcgatca
     8581 cggggaagac tgcctcccag gcgcgcaccc tgccgctcag gaacaagaac ctgagggcgc
     8641 ccacgttcgt gaccatgtag gctatcagca tgctcagcac ccctatagtg cccggataga
     8701 agaaagcgtt caccgcggag acgccgcct
//
LOCUS       DATMJA010000342         5191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_638208, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000342 DATMJA010000000
VERSION     DATMJA010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638208"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VNT12_08205"
     CDS             <1..306
                     /locus_tag="VNT12_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HWI46141.1"
                     /translation="APEIQERETGTAEVRQTFRVPNVGVVAGCYVTSGEIFRNNRVRV
                     VRDGAVVYEGNIASLKRFRDDVRSVREGFECGVGIENFNDVKVGDVLEFFETVEVPR"
     gene            316..597
                     /locus_tag="VNT12_08210"
     CDS             316..597
                     /locus_tag="VNT12_08210"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWI46142.1"
                     /translation="MFCAVRLELELPFSSSLKDKRQTLRSLKDRLRRKNVSVVESDHQ
                     DLWQRATIEIALAAVSRGAAEEKREELRRTLLGYEELSILDWREEFVKL"
     gene            594..953
                     /gene="rbfA"
                     /locus_tag="VNT12_08215"
     CDS             594..953
                     /gene="rbfA"
                     /locus_tag="VNT12_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWI46143.1"
                     /translation="MSGRTRKVESQLKEITGEEVASLSDPRIQGLVTVTGVRVSPDLA
                     HATVYYSVLAGEDEMAANEGLQSAAGRIQAAVGAQMRLKRTPRLRFEPDPIVERATRI
                     EAALREVRTDEDTHEAE"
     gene            928..1893
                     /locus_tag="VNT12_08220"
     CDS             928..1893
                     /locus_tag="VNT12_08220"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HWI46144.1"
                     /translation="MKTRTKQNNTLPEIAEFLSSLDHVAVTTHVGSDGDAIGASAALV
                     RLMRRLGATAVFCHAEGVPEYLGWLLPDEPLRELPPDHDLLVADTSRADRTGVPVPES
                     GARLNIDHHEDNPLYAEYNLVSPRAAATAEIVADLYAELDVPIDRPTAEALYVGIRTD
                     TGGFNFRNISPRAHELVADLLRAGVVPAEVNERINRRGSIEQLRIVGVSLANAVRYGE
                     ALISTVENSDYERTGATEIDSKESIDQLRTVAGVDVVAHLREVPQGTKGSLRSETVDV
                     GEIARRFGGGGHRLAAGYTTPRRPDEAREELLGILEGVLDLGGGR"
     gene            1923..2771
                     /gene="truB"
                     /locus_tag="VNT12_08225"
     CDS             1923..2771
                     /gene="truB"
                     /locus_tag="VNT12_08225"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWI46145.1"
                     /translation="MDKPPGITSARAVSGVKRLLPRKTRIGHTGTLDPLASGLLVLLV
                     GRATRLSRYVTNLEKSYTATARFGAVSDTLDAEGYITDLDVPLPPEEALRDALPAFTG
                     DLLQVPPMASALKQRGVRLYDLHRRGISVEREARPVTVHSLELASLDPTRQTAVFEIS
                     CTSGTYIRTLINDLATSLGSGAYLTALRRTSVGHLTVDNASSSQDLLPETLHNHIIPS
                     LDVIAHLPEVAVSEETGRGLVCHGRRIERGRVDGSYRVMYGGELLAVYRDDEDAGRAE
                     VVLCAP"
     gene            2759..3655
                     /locus_tag="VNT12_08230"
     CDS             2759..3655
                     /locus_tag="VNT12_08230"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWI46146.1"
                     /translation="MCPVVVALGNFDGVHLGHQEVLRRAVEEGRRRSMKVVAATFHPH
                     PRTVLGAGDPPKLLTPLELRCEMLLSYGADEVWVIPFDVALSRKTPEQFVKDVLIGEG
                     GAGVVVVGENFRFGHKAAGHFSDLLRLMQKAGGTAVGVQVRGGGMEGGISSTRIRALV
                     SGGEVAEAAGFLGRPYVLRGEVVVGDKRGRSIGFPTANVLPDVDAVIPARGVYAGFVR
                     FGEEEYGACTNVGVAPTFGRTESRIEAYLLDFKGDLYGRVVDLSFVKRIREERRFSGV
                     DELTEQIRRDVKEARIIAGGPG"
     gene            3759..4025
                     /gene="rpsO"
                     /locus_tag="VNT12_08235"
     CDS             3759..4025
                     /gene="rpsO"
                     /locus_tag="VNT12_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWI46147.1"
                     /translation="MAVVEHKEEIIQEHRTHEGDTGSPEVQVAVLTRRIGHLTEHLRE
                     HKHDYHSQRGLLKMVGRRRRLLKYLQKKDVERYRALIAKLGLRR"
     gene            4061..>5191
                     /locus_tag="VNT12_08240"
     CDS             4061..>5191
                     /locus_tag="VNT12_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWI46148.1"
                     /translation="MRLDIQVGERSIVLETGKLAKQAGGSVTARLGDTMILSTATRSQ
                     APRPGATFLPLSVDIEERMSSAGKIPGGFIKREGRPSERAILTSRLTDRPLRPLFPKN
                     YHYEIQVIGTVLVADQDTPYDTVSMVGASAALALSDIPFYGPIGAVRVGRSLDGEFIL
                     NPTYAQIAESDLDLVVAGTKEAITMVEAGANEVPENVIVEALQLAQGAVKAQAEAIEE
                     WAAEYGEEEQEIEVAEEHPFLVDFRGRYFDRIKEGIVQTDRRARHDVLQELREELIED
                     REEEEIPQITAALSTLEKEAFRALYLEDRKRTDLRGLDEIRPTSAEVQVLPRVHGTGL
                     FTRGETQVLSSLALADLGLAQRLDTIEPLTGKRYMHHYNFPPF"
BASE COUNT         1044 a         1586 c         1690 g          871 t
ORIGIN      
        1 gcccccgaga tacaggagcg ggagaccggc accgccgagg tacgccagac cttcagggtt
       61 cccaacgtcg gtgtggtagc aggctgctac gtcactagcg gggaaatctt ccgcaacaac
      121 cgcgtgcgcg tggtgcgaga cggcgccgtg gtctacgagg gcaacatagc ctccctgaag
      181 cggttcaggg acgatgtccg ttcggtacgt gagggtttcg agtgcggcgt cggcatcgaa
      241 aacttcaacg acgtcaaagt gggagacgtt ctggagttct tcgagaccgt cgaggtcccg
      301 aggtagagtc gacaattgtt ctgtgcggta aggctggaac tggagctgcc tttctcttcg
      361 agcctgaagg ataagcgaca gacgcttcgc tccctcaagg accggctgcg ccgcaagaac
      421 gtctccgtgg tggagtcgga tcaccaggac ctctggcagc gcgccacgat agagatcgca
      481 ctcgccgcgg tctcgcgcgg ggcagccgag gagaagcgag aggagcttcg gaggacgctc
      541 ttaggctatg aggagctctc tatactggac tggcgcgagg agttcgtaaa gctgtgagcg
      601 gtcgcacacg caaggtggag tcgcagctaa aggagatcac cggcgaggag gtagcctcgc
      661 tctcggaccc gcgcatccag ggcctcgtca ctgtgaccgg cgtcagggtg agccccgacc
      721 tcgcccacgc tacggtgtac tacagcgtgc tcgccggcga ggatgagatg gccgcaaacg
      781 agggcctgca gagcgccgcc ggccgcatcc aggccgcggt aggggcccag atgcgcctca
      841 aacgcacgcc aaggctgcgc ttcgagcctg accctattgt cgagcgcgcc accaggatag
      901 aggccgcatt gagggaggtt aggacagatg aagacacgca cgaagcagaa taatacgcta
      961 ccggagatag cggagttctt gagctccctg gaccacgtcg ccgtaacgac tcatgtaggt
     1021 tcagacggcg acgccatcgg cgcttcggcc gctctcgtgc gtctcatgcg ccggctgggg
     1081 gccacggccg tattctgcca cgcggaaggg gtgcccgagt atctgggctg gctcctgccc
     1141 gacgaacccc tgcgcgagct tccgccggat cacgaccttc tcgtcgccga cacatcccgc
     1201 gcagaccgaa ccggcgtacc cgtgccggaa tcgggcgccc gcctcaacat cgaccaccac
     1261 gaggacaacc ctctctacgc cgagtacaac ctggtgagcc cgagggccgc cgcgacggct
     1321 gagatcgtcg ccgatctcta cgccgagctc gacgttccta tcgacaggcc gaccgccgag
     1381 gccctctacg taggcatcag aaccgacacg ggcggcttca acttccgtaa catctccccc
     1441 cgcgcacacg agctcgtcgc cgaccttctt cgggccggcg tcgtgcctgc cgaggtcaac
     1501 gagcgcatca atcgcagggg cagcatcgaa cagctcagga tcgtcggcgt ctcgctcgcg
     1561 aacgccgttc gttacgggga ggccctgatc tccacggtcg agaactcgga ctatgagaga
     1621 accggcgcaa cggagatcga ctcgaaagaa tccatcgacc agctccgcac cgtcgccggg
     1681 gtcgacgtcg ttgcccacct ccgcgaggtg ccgcagggta cgaagggttc cctccgctcc
     1741 gagaccgtcg acgtcggaga gatagcgcgt cgctttgggg gaggcggcca ccggctcgcc
     1801 gccggataca ccacgccgcg ccgccccgac gaggccaggg aagagcttct gggaatactg
     1861 gaaggggtcc tagatctcgg aggcgggcga taacggagag ggcgctctca ggcggcctcc
     1921 tgctggacaa gccgccaggc ataacgagtg ccagggcggt ctccggggtc aagcgcctgc
     1981 tcccgaggaa gaccaggatc gggcacacag gaacccttga ccccctggcc tcgggccttc
     2041 tcgtgctcct cgtcggacgc gcgacccgtc tgtcgcgcta cgtgaccaac ctcgaaaagt
     2101 cctatacggc caccgcccgc ttcggcgccg tctcggatac gctcgacgca gaaggctata
     2161 tcaccgacct cgacgtccct ctccctccgg aagaagctct tcgcgacgcg ctccccgcct
     2221 ttacgggtga cctcctccag gttccgccga tggcctcggc cctcaaacag agaggcgtca
     2281 ggctctacga tttgcaccgc cgcggcatca gcgtcgagcg ggaggcacgt cccgtcacgg
     2341 tacatagtct cgaactcgcc agcctcgacc ccacaagaca gacggcagtg ttcgagatct
     2401 cctgcaccag cggtacctac ataagaaccc tcataaacga cctcgccaca tccctcggct
     2461 caggcgccta cctcaccgcc ctgcgccgca cgagcgtcgg acacctcacc gtagacaacg
     2521 ccagttcctc ccaagatctc ctccctgaga ccttacataa ccacataata ccatcgctcg
     2581 atgtgatcgc ccatctccct gaggtggctg tttcagagga aacgggccgg ggtctggtat
     2641 gccacggcag gcgcatagag aggggccggg tcgatggcag ttaccgggtg atgtatgggg
     2701 gcgagctctt ggccgtgtac agggacgacg aggacgcggg gcgggccgag gtggtgctat
     2761 gtgccccgta gtggtagccc tcgggaactt cgacggcgtg cacctcggcc accaggaggt
     2821 cttgaggcgg gccgtcgagg aggggcggcg gaggagcatg aaggttgtag cggccacctt
     2881 ccacccgcat ccacggaccg tattgggggc tggtgatccg ccgaagctgc ttacgccttt
     2941 agagttgcgg tgtgagatgc tgctcagtta cggggctgat gaggtgtggg tgataccgtt
     3001 cgatgtcgcc ctgtccagga agaccccaga gcagttcgta aaggacgtgc tgattggcga
     3061 gggtggggcc ggggtggtcg tagtaggcga gaactttcgt ttcgggcata aggctgccgg
     3121 ccacttcagt gacctcctgc ggctcatgca gaaggcgggt ggaactgccg tcggcgttca
     3181 ggtacgcggt gggggcatgg agggtgggat cagctccacc cgcatacggg ctctggtctc
     3241 cggtggagaa gtggccgagg ccgccggctt ccttggacgt ccctacgtac tcaggggcga
     3301 ggtagtggtc ggagacaagc gcggcaggtc gatcggcttc ccgacagcca acgtgctgcc
     3361 cgatgtggac gccgtgatac cggcccgcgg agtctacgcc ggattcgtcc gcttcggcga
     3421 agaagaatat ggcgcatgta caaatgtcgg ggtggctccc accttcgggc gcaccgagag
     3481 ccggatcgag gcctatctcc tcgactttaa gggggatctc tacgggcgcg ttgtagacct
     3541 cagcttcgtg aagcgtatta gggaggagcg acgattctcg ggcgtcgacg agctcacaga
     3601 gcagattcgg cgcgacgtga aggaggccag gatcatagct ggtggaccgg gctgacctgg
     3661 ggtctttggc acacgatact aattgatgca tgtggtagca taaagggtcg aacgagttaa
     3721 ccttaagaga gaagtgaaca aagaggagaa agaagaagtt ggctgtagtt gagcacaaag
     3781 aggagatcat tcaagagcac cgcacgcacg agggggacac ggggtctccc gaggtgcagg
     3841 tcgcggtgct cacgcggcgc ataggtcacc ttacggagca cttgcgcgag cacaagcacg
     3901 actaccattc gcagcgtgga ctgctgaaga tggtcggcag gcgccgcagg ttactcaagt
     3961 atctgcagaa gaaggacgtc gagcgctacc gggcgctcat cgccaagctc ggcctacgcc
     4021 ggtagtccgg gtaacaagaa aagcaagagg agaaaacaac atgcgactag acatacaagt
     4081 aggtgagcgt tcgatcgtgc tcgaaaccgg gaagctagcc aagcaagccg gtggctcggt
     4141 gacggcgaga ctcggcgaca ccatgatact ttcaactgcg acgcgctcgc aggccccgcg
     4201 cccgggcgcg acgttcttac cccttagcgt ggacatcgag gagcgcatgt cctcggcggg
     4261 taagatccct ggcggcttta tcaagcgcga gggtaggccc tcggagcggg ctatcctgac
     4321 ctcgcgcctc acggataggc ctctcaggcc gctcttcccc aagaactacc actacgagat
     4381 acaggtaata ggcaccgttc tggtcgccga ccaggatacc ccatacgaca ccgttagtat
     4441 ggtcggggct tctgcggcgc tcgccctctc cgacataccc ttttacggcc cgatcggggc
     4501 cgtacgcgtc gggcgttcgc tggacggcga gttcattttg aatcctacat atgcccagat
     4561 tgccgagagc gatctggacc tcgtggtcgc agggacgaag gaagccataa ccatggtcga
     4621 ggccggggcg aacgaagtgc ccgagaacgt catagtcgag gccctgcagc ttgcgcaggg
     4681 ggccgtcaag gcccaggcag aggcaataga agagtgggcc gcagagtacg gagaagagga
     4741 gcaagagata gaggtagccg aggagcaccc gttcctcgtg gactttcgcg ggcggtactt
     4801 cgaccggatt aaagagggca tcgtccagac cgatcgaagg gcccgtcacg acgtgctcca
     4861 ggagttgcgt gaagagttga tcgaggaccg cgaagaagag gagatacccc agatcaccgc
     4921 ggccctctcg acactcgaga aggaggcttt ccgcgcgctc tacctcgagg accgcaagcg
     4981 tacggatctg cgtgggctgg acgagataag gccaacctca gccgaagtgc aggtacttcc
     5041 gcgggtccac ggtacggggc tctttacgag gggcgagacg caggtcttga gctctctggc
     5101 tctggccgac ctcggtctgg cgcagcggct cgacaccatc gagccgctga cgggcaagcg
     5161 ctacatgcac cactacaact tcccgccctt c
//
LOCUS       DATMJA010000343         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_638331, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000343 DATMJA010000000
VERSION     DATMJA010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638331"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /gene="trkA"
                     /locus_tag="VNT12_08245"
     CDS             <1..1123
                     /gene="trkA"
                     /locus_tag="VNT12_08245"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HWI46149.1"
                     /translation="ASSSDEVNIIACLAAKAQGVSRTVARIHNPDYYESGEPFAREML
                     GIDFIIHTEQMAALEIKEALLVPGAINVDSFAEDTIEVAEVILNEGSEAVGRTLSDVR
                     LPDESLIVGVVRGGEALVPKGGTVLEQRDHVLLISGRRQISEAVGALATDTAPVRDVT
                     IYGGGRIGLRLALSLEKAGMSVRVIESDEGRARYVASQLRRGFVLHDEGISRDFLLQE
                     GVDRTDAFVAVTGDDRANLLAAMYARQLGARMTVAGISRGEFAPLADALGVDLTISPR
                     MLAAEAILRFVRKGEVIDVALLASGAEMIELRVPERCRVAGRPLSEVGFPEGAIVGAL
                     LRKGTVVIPTGKEILRPGDDAVVFTVEDAVEEVENLFAT"
     gene            1127..>2384
                     /locus_tag="VNT12_08250"
     CDS             1127..>2384
                     /locus_tag="VNT12_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HWI46150.1"
                     /translation="MNPRVVIRVNAGVILAIGLALLVPLALSVLYRDGSWTSFLLPAT
                     LMVAVGIVCFRLSGPRGRAPEYVSNRDVYLSVTLAWTLAALLGGTPFLVEGTFHSLLD
                     STFEAMSGFTTTGATLLSDIEAQTPSILFWRSMTHWLGGIGIVVLFVAVAPLLGVGAA
                     RLIGAEMSGLTQPRLTPRIADTAKALVIIYLAISVAETVALLLARMPLYDAVVHTFGT
                     VATGGFSNKTASVGFYDSLAIEAIIGFFMVASGVSFSLYYLLFTRRRLDVLLDRELLA
                     YLGIIAAASLFIWGILALEGDYGDSWGRALRDSAFAVSSIITTTGFITADFDRWDAAA
                     KITLVLLMFIGGCAGSTAGGIKVIRILIIFRTILQDVFRMIHPRAVTPLQIGGRVVPE
                     TVRIAVLGLFAAWIGVFGIATFLVALQ"
BASE COUNT          385 a          758 c          787 g          454 t
ORIGIN      
        1 tgcgagtagc tcagacgagg tgaacatcat cgcttgcctg gcggcgaagg cccagggagt
       61 ctcccgcacc gtggcgcgca tacacaatcc cgattactac gaatcgggag agcctttcgc
      121 cagagagatg cttggtatcg acttcataat ccatactgag cagatggcgg cgctggagat
      181 caaggaagcg ctgctggtgc ccggcgcgat caacgtggat agcttcgccg aggacaccat
      241 cgaagttgcc gaggtaatcc tgaacgaggg ctcggaggcc gtgggccgca cgctcagcga
      301 cgtgaggttg cccgacgaga gcctcatagt cggcgtcgtg aggggcgggg aggcgctcgt
      361 gccgaagggt ggaacggtgc tggagcagag ggaccacgtc cttctgatca gcggccgaag
      421 gcagatttcc gaggccgtgg gcgccctggc cacggacacg gcgccggtaa gagatgtgac
      481 catctacggc ggaggacgca tcggcttgcg gctggccctc tcgctggaga aggcaggcat
      541 gtcggttagg gtcatagaga gtgacgaggg cagggcccgc tacgtggctt cccagctcag
      601 gaggggtttc gtgcttcacg acgaggggat ctcccgcgat ttcctcctcc aggagggcgt
      661 ggaccgaacc gacgccttcg ttgccgtcac tggggacgac agggccaacc tgctggccgc
      721 catgtacgcc cgccagttgg gggcccgcat gaccgtcgct ggcataagcc gcggcgagtt
      781 cgcgccgctc gcggacgccc tcggggtaga cctgacgatc tccccgagga tgctcgcggc
      841 cgaggccata ctccgcttcg tacgcaaggg ggaggtcatt gatgtcgccc tgctggcaag
      901 cggggccgag atgatcgagc tcagggtccc cgagcgatgc agggtcgctg gccgcccgct
      961 ctctgaggta gggttccccg aaggcgctat cgtgggggct ctgctgcgca aagggaccgt
     1021 ggtcatcccg acgggcaagg agattctcag gccaggcgac gacgccgtgg tgttcaccgt
     1081 cgaggatgcc gtggaagagg tcgagaacct cttcgccacc tagcctttga accccagggt
     1141 cgtcatcagg gtgaacgccg gtgtgatcct ggcgatcggg ctggcgctcc ttgtccccct
     1201 cgcgctctct gtgctctacc gcgacggctc gtggacgagc ttcctgcttc ctgcaacgct
     1261 gatggtcgcg gttggcatcg tatgcttccg cctctccggg cctcggggcc gcgcacctga
     1321 gtacgtgtcg aacagggacg tatacctctc cgtaacgctg gcctggacgc tcgccgccct
     1381 gctgggtggt acgccgttcc tcgtcgaggg caccttccat agccttctgg actccacctt
     1441 cgaggcgatg agcgggttca cgaccacggg ggccacgctg ctctcggaca tcgaggccca
     1501 gacgccctcc atactgttct ggcggagcat gacccactgg ctcgggggaa tagggatcgt
     1561 ggtcctcttc gtcgcggtcg caccgctcct tggcgtcggg gcggcgcgcc tgatcggggc
     1621 tgagatgtca gggctcacgc agcctcgcct gacgccccgc atcgcggaca cggcaaaggc
     1681 cctcgtgata atctacctag ccatctcagt cgccgagacc gtcgcgctgc tacttgcgag
     1741 gatgccgctt tacgacgccg ttgtccacac gttcggcacc gttgcgacgg ggggtttcag
     1801 caacaagacc gcgtctgtcg gcttctacga ctcgctcgcc atagaggcga tcatcggctt
     1861 tttcatggtg gcctcgggcg tctcgttctc gctctactac ctcctcttca ccaggcgccg
     1921 gctcgacgtg ttgctcgaca gagaactgct ggcttacctc ggaataatcg ctgcagcgtc
     1981 actcttcatc tggggcatcc tcgccctgga aggcgactac ggggattcct ggggcagggc
     2041 gctcagggac tcggcgttcg cggtctccag cataatcacc acgacgggat tcataacggc
     2101 cgacttcgac cggtgggacg cggccgccaa gatcaccctc gtcctgctca tgttcatagg
     2161 gggatgcgcc ggttcgaccg cgggcgggat caaggtcatc cgcatcctca tcatcttccg
     2221 cacgatcctc caggacgtct tcaggatgat ccatccgcgc gcggtcactc cgcttcagat
     2281 aggcgggcgc gtggtgcccg agaccgtgcg catcgccgtc ctcggcctct tcgcggcctg
     2341 gatcggggtc ttcggtatag cgacgttcct cgtggccctg cagg
//
LOCUS       DATMJA010000344         6093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_460846, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000344 DATMJA010000000
VERSION     DATMJA010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460846"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VNT12_08255"
     CDS             <1..327
                     /locus_tag="VNT12_08255"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HWI46151.1"
                     /translation="VQTEVPEGTSVWLSSRDKEKVSNGFDRMATQIKNQLGGEKPKLV
                     FQFECGTRGKMMFREQEKLQILRRFRQSLDPDAPWVGFYTVGEIGPVEEHNLRHLYTS
                     VVLALG"
     gene            complement(405..1694)
                     /locus_tag="VNT12_08260"
     CDS             complement(405..1694)
                     /locus_tag="VNT12_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HWI46152.1"
                     /translation="MREDLQEIFEAGLTAADPQRAVRRALELEDGAILAGEKRFEPRR
                     VFVLAAGKAAGAMAQAAEELLGEKISGGLVVTKDGHDPGPDSFETIFASHPEPDERSV
                     EAARKVQEVVESLEEEDLLLALISGGASALLADPAPPIELADLKKLTSDLLKSGADIG
                     EINTVRKHVSVLKGGGLVRLAHPAPTIALLLSDVVGDEPSSIASGLTAPDPTTLDNTR
                     RVLERYGIEPPDSVADHLQNAEETPASDDPIFENVVNCICGGGRHAAEAAAKKAGELG
                     YTPLLLTTTLTGDALGAASMYAAIIREVLASGNPIAPPCAIVSGGEATVTVRGSGTGG
                     PNQEFALALAVELDGVEGWTAFSADTDGNDGSTDAAGGIVDGKTSKRIRDAGMDSEGA
                     LSDNDSYAALEAGEALVVTGPTGTNVNDVRVALVSTR"
     gene            1764..2570
                     /locus_tag="VNT12_08265"
     CDS             1764..2570
                     /locus_tag="VNT12_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWI46153.1"
                     /translation="MRFSANVSILYKEVPLLERFGRAAAAGFSAVEFWWPGHEAEPGA
                     VERAARDAGLDVALFNFDAGDMAAGDRGLVSDPERQHLFRDNVPVALDLARALECTRM
                     NVLVGHEIPGMDREEQLALARENVRFAAEKAEAANVTVMVEAVNTFENGPYLLYTTEQ
                     AVEFVERVRRENVRIQHDFYHMQRMEGNLVANLREHRDHIGHVQIADSPGRGEPGTGE
                     IHYPFVLGELERLGYDGYVGLEYNPTTETTEESFGWLPKELRGRDADLGL"
     gene            2582..3481
                     /locus_tag="VNT12_08270"
     CDS             2582..3481
                     /locus_tag="VNT12_08270"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HWI46154.1"
                     /translation="MADAVGFIGLGIMGRPMAKNLMDAGYELVVHNRSPEKAEELAEE
                     GNATAAGSPREVAEACHIVITMLPDSPDVEAVVAGEDGVLEGIRDGALLVDMSTISPV
                     VTEELSEKVRKQGASMLDAPVSGGDVGAIEGALSIMVGGSEEDFERARPLFDVMGKVA
                     THVGPTGAGQVVKACNQIVVALTIEALSEALVLGSKGGVAPEKLVEALSGGLAGSAVM
                     EAKKEKFFSHDFEPGFRIELHHKDLGIALAAGREYGVALPVTAIVDQMLEASKAKGRG
                     DRDHSALLTLLEEAAQHKIGEGG"
     gene            3507..4961
                     /locus_tag="VNT12_08275"
     CDS             3507..4961
                     /locus_tag="VNT12_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWI46155.1"
                     /translation="MERSRLIREMEGIVGAEGVIHEHEQLRTYECDGLMNYRVIPDLV
                     VLPETGEHVQKVVKLCYDEGIPFVARGSGTGLSGGALPVESGVLIVLSRMRKILEVDI
                     PNQRIVVEPGVVNMWVSQEVADEGYFYAPDPASQIVSSIGGNLAENSGGVHCLKYGFT
                     TNHVMGAEVVLPDGSVTHIGGGRTPDAPGYDLLGAFVGSEGTLGIATRITLRLCRVPE
                     AVRTLLAGFEDTEGAGGAVSGIIGEGIIPAAIEMMDSLTMEAVEAAVHPGLPEAGAVL
                     VIELDGPEAEVEEQFGRVLEICKENEATEIRVAEDDDERALFWKARKAAFGAMGRISN
                     DYYVQDGVIPRTELPAVLGKISELAGRYGLRVANVFHAGDGNLHPLVLYNNDTEGEAE
                     NAEKLAGEIVFACLEHGGSLTGEHGIGEDKKKYMPRMFTDDDMNVMQLLRCAFDPHQL
                     ANPGKIFPTPRLCGERPGPFHMHPLQKAGIVEQF"
     gene            4961..>6093
                     /locus_tag="VNT12_08280"
     CDS             4961..>6093
                     /locus_tag="VNT12_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWI46156.1"
                     /translation="MQTEKGLTEDLRRIVGPENVHEATEQDAVEGVEPDFVVEPGSVE
                     EISDVMKLAARDGLAVAPRGSGTKMHFGDPPRRLDLIVSTARMNEVLEHTPGDQIVRL
                     QAGVKLEDLQEYVSGSDQMLAIDPPESGATVGGIVAANSSGPRRYRYGTIRDLIIGIT
                     VVLHDGTVAKAGGKVVKNVAGYDLSKLFTGSLGTLGIIATANFRLHPRHEASRTVAVE
                     VTEPQQAQAAAQAIVHSQVEATAVELHYGENEKLLAVLLESIPGGIGAKAETASFLLK
                     QFGEVRTLSDEEADHVGPLTPPEVADEVVLKIGAPPVDLAAVLESVLGAAERKGLAHP
                     RITGRAGTGVTLVGFSEENEDGAAGFVEEMREIWVRRGGNVTL"
BASE COUNT         1153 a         1844 c         2063 g         1033 t
ORIGIN      
        1 gtacaaacgg aggttccaga aggcacgagc gtctggctat cgagccgaga caaggagaag
       61 gtctccaatg ggttcgatag gatggcaacc cagatcaaga accaacttgg gggtgagaaa
      121 cccaaactag tgttccagtt cgagtgtggc accagaggga agatgatgtt ccgtgagcag
      181 gagaagctgc agatcctaag gcggtttcgg caatccctag atccggatgc accgtgggtg
      241 ggtttttaca ctgtgggaga gatcggtccg gtagaggagc acaatcttcg tcatctctac
      301 acgtcagtcg tgcttgctct aggttgacgg tagaaggagc catactagcg aaacgcgttt
      361 gattgcactt tgcgtgaccc ggcgcaagcg cccgagacgc cacctcagcg ggtagagacg
      421 agcgccaccc ttacatcgtt gacgttggtg cctgtcggcc ccgtgacgac caaggcctcg
      481 cccgcctcca gcgccgcgta cgagtcgtta tctgacagcg ccccctccga gtccatgccg
      541 gcatccctga tcctcttcga cgtcttcccg tcaacgatcc cgccagccgc gtcggtcgat
      601 ccgtcgttgc cgtcggtgtc ggccgagaat gccgtccagc cctcgacacc atccagctcg
      661 accgcgagcg ccaaagcgaa ttcctggttc ggtccgccgg tgccgcttcc gcgcacggtc
      721 accgtcgcct caccgcccga gacgatggcg cacggtggcg cgatggggtt gccgctcgcc
      781 aatacctctc taattatcgc cgcgtacatc gaggccgccc cgagggcgtc gcctgtgagg
      841 gtggtggtga gcagcagcgg tgtatatcca agctctccag ccttcttcgc cgcagcctcg
      901 gccgcgtgcc tgccgccgcc gcagatacag ttaacgacgt tctcgaagat tgggtcgtcc
      961 gatgccgggg tctcctcagc attctgcagg tgatctgcga cgctatccgg tggttctatg
     1021 ccgtagcgct ccagcacgcg cctggtgtta tcgagggtcg tcgggtcagg ggccgtgagg
     1081 ccgctcgcga tggaagaggg ctcgtcgcca accacgtccg agagcaggag ggcgatggtc
     1141 ggggccggat gggccaaccg caccaaaccg ccgcccttta atacagagac gtgcttcctt
     1201 acggtgttga tctcccctat atccgccccg ctcttgagga ggtcggacgt aagcttcttc
     1261 aggtccgcaa gctcgatagg aggagccggg tcggcgagca gcgccgaagc cccacccgag
     1321 atgagagcga gaagcagatc ttcctcttcc agggactcga ccacttcctg caccttccgg
     1381 gccgcctcta cgcttctctc gtcgggctcg ggatgggagg cgaagatggt ctcgaagcta
     1441 tcgggacctg gatcgtgacc gtctttggtg acgacgagtc cgcctgagat cttctcccca
     1501 agaagctcct cagcggcctg agccatcgct cccgccgcct tgcccgccgc gagtacgaac
     1561 acgcgccgcg gctcgaaacg cttctctcca gccaggattg cgccgtcttc gagttctaac
     1621 gcgcgccgca cggcccgctg cgggtcggcg gcggtgagac cggcctcaaa tatctcctgg
     1681 aggtcctcgc gcacaagggc attgtaacca tccgggagtt cggcgcccgg ttgggttatg
     1741 cttgtggcgg aaggagggtt ggcatgaggt tcagcgcgaa cgtttccatc ctgtacaagg
     1801 aggttccgtt actggagcgg ttcgggcggg cggccgcagc aggcttctcg gcggtcgagt
     1861 tctggtggcc cggccatgag gcagaaccgg gggcggtgga gcgcgccgcc cgagacgccg
     1921 ggctcgacgt ggcgctcttc aacttcgatg ccggagatat ggcggccgga gacaggggac
     1981 tggtgagcga tcccgagcgt cagcatctgt ttagggataa cgtgccggtc gccctggatc
     2041 tggcgcgtgc gctcgaatgt acgaggatga acgtgctcgt ggggcatgag attcccggca
     2101 tggaccgcga agagcagctc gcgctggcgc gcgaaaacgt acgcttcgcc gcagagaagg
     2161 cagaagcagc aaatgtcacg gtcatggtcg aggctgtcaa caccttcgag aacgggccct
     2221 atctgctgta cacgacggag caggccgtag agttcgtaga gcgtgtcagg cgggagaacg
     2281 tgcggataca gcacgacttc tatcacatgc agcggatgga agggaacctg gttgccaacc
     2341 tgcgcgagca ccgcgaccac atcgggcacg tccagatcgc agattctcca ggccgcggtg
     2401 agccggggac gggggagatc cactacccgt tcgtcctcgg cgaactggaa cggctcggct
     2461 acgatggcta cgtcggcctg gagtacaacc cgaccacaga gacgaccgaa gagagcttcg
     2521 gctggttgcc gaaggaactg cgcggcaggg acgctgacct gggactttag agaggagcaa
     2581 catggctgac gcggtaggtt ttatagggct tgggatcatg ggcaggccga tggcgaagaa
     2641 cctgatggac gcgggctacg agctggtcgt ccacaaccgc agcccggaga aggcggagga
     2701 actggcggag gaggggaatg ccaccgccgc cggaagcccg agggaggtgg ctgaggcgtg
     2761 ccacatcgtc atcaccatgc tccccgattc accggacgtt gaggccgtgg tggccggaga
     2821 ggacggtgtc ctcgaaggca tccgcgacgg tgcgctgctc gtggacatga gcaccatctc
     2881 gccggtagtc accgaggaac tttcggagaa ggtccgcaaa cagggtgcct cgatgttgga
     2941 tgcgccggtc tccggaggcg acgtgggggc tatagagggc gcactgtcca tcatggtcgg
     3001 tggttcggag gaggacttcg aacgcgcccg cccactattc gacgtgatgg gcaaggtggc
     3061 gacgcatgtg gggccaaccg gggcagggca ggtggtcaag gcatgcaatc agatcgtggt
     3121 cgcgcttacc atcgaggctc tctccgaggc gctcgtgttg ggctccaagg gcggggtcgc
     3181 gcctgagaag ctggtggagg cgctctccgg ggggctggcg ggcagcgcgg tgatggaggc
     3241 caagaaggag aagttcttct cacacgactt cgaaccgggt ttcaggatag aattgcacca
     3301 caaggatctg ggtatagccc ttgcagcggg cagggagtac ggcgtggccc taccggtcac
     3361 ggccatcgtg gaccagatgc tcgaagcgtc gaaggccaag ggccggggcg accgggacca
     3421 ctcggcgcta ctcaccctgc tggaggaagc cgcgcaacac aagatagggg agggaggctg
     3481 agacttccca ccgaaggagg gcacacatgg agcgtagccg gctcataagg gagatggaag
     3541 gcatcgtggg cgcggagggc gtgatccacg agcacgagca gctacgcacc tacgagtgcg
     3601 acggcctgat gaactaccgc gtcatccccg acctcgtcgt cctccctgag accggcgaac
     3661 acgtccagaa ggtagtcaag ctctgctacg acgaggggat tccctttgtt gcccgcggct
     3721 cggggaccgg cctctcgggt ggggccttgc ctgtcgagtc gggcgttttg atcgtgctct
     3781 ccaggatgcg caagattctg gaggtagaca tcccgaacca gcgcatcgtg gtcgagcctg
     3841 gcgtcgtcaa catgtgggtg agccaggagg tcgccgacga gggctacttc tacgccccag
     3901 acccggccag ccagatcgtc tcctccatcg gcggcaacct tgccgagaac tccggcggcg
     3961 tccactgcct gaagtatggc tttaccacga accacgtgat gggtgcggag gtcgtgctgc
     4021 ctgacggctc ggtgacgcac atcggtggcg gccggacgcc ggatgcacct ggctacgacc
     4081 tgctcggcgc ttttgtcggc tcggagggga cgctgggtat cgccaccagg atcacgctcc
     4141 ggctgtgccg cgtccctgag gcggtccgaa ccttgctggc cggcttcgag gataccgagg
     4201 gggccggcgg tgcggtctcg gggatcatcg gcgaggggat aatcccggcc gccatcgaga
     4261 tgatggacag cttgaccatg gaggccgtcg aggcggccgt gcatccaggc ctgcccgaag
     4321 ccggcgcggt gctcgtcatc gagctggatg gacctgaagc tgaggtagag gaacagttcg
     4381 gacgggtgct ggagatctgc aaggagaacg aggccacgga gatccgggtg gccgaggacg
     4441 acgacgaacg cgcactcttc tggaaggccc gcaaggccgc tttcggtgct atgggccgca
     4501 tctccaacga ctactacgtc caggacggcg tcatcccgcg cacggagctc cctgcagtcc
     4561 tgggtaagat ctccgagctc gccgggaggt acggcctgcg ggtcgccaac gtcttccacg
     4621 ccggggacgg aaatttgcat cccctggttc tctacaacaa cgacaccgag ggcgaggccg
     4681 agaatgccga gaagctggcg ggcgagatcg tcttcgcctg cctggagcac ggtgggtcgc
     4741 tcaccgggga gcacggcatc ggggaggaca agaagaagta tatgcccagg atgttcaccg
     4801 atgacgacat gaacgtcatg cagctcctgc ggtgcgcctt cgatccgcat cagttggcca
     4861 acccgggcaa gatcttcccc accccccgcc tctgcggcga acgtccaggc cccttccaca
     4921 tgcaccccct gcagaaggcc ggcatcgtgg agcagttctg atgcagacgg agaaaggact
     4981 cacagaggac ctgagacgca tagtcggccc ggagaacgtc cacgaggcta cggaacagga
     5041 tgccgtcgag ggcgtcgagc ccgacttcgt ggtcgagcct ggatccgtcg aggagatcag
     5101 cgacgtaatg aagctcgccg cccgcgatgg cctggccgtc gcgccccggg gcagcggcac
     5161 gaagatgcac ttcggcgacc cgccgcgtcg gctcgacctc atcgtaagca ccgcacgaat
     5221 gaatgaggtg ctggagcaca cgccaggaga ccagatcgtc cgtctccagg ccggggtgaa
     5281 gctagaagac ctgcaggaat acgtctccgg ctcggaccag atgctcgcca tagacccgcc
     5341 ggagagcggg gccactgtag ggggaattgt cgcggccaac tcgtccgggc caagacggta
     5401 caggtacggc accataagag acctgatcat cggcataacc gtcgtcctgc acgacggcac
     5461 cgtggccaaa gcaggcggca aggtcgtcaa gaacgtggcc ggctacgacc tgtcaaagct
     5521 cttcaccggc tccctgggta ccctgggtat catcgccaca gccaacttcc gcctccaccc
     5581 gcgtcacgaa gcttcacgca ccgtggccgt cgaggtcact gaaccacagc aggcgcaggc
     5641 ggcggcacag gctatagttc attctcaggt ggaggctacg gcagtcgagt tgcactacgg
     5701 cgagaatgag aaactcctgg cagtgcttct ggagagcatc cccggtggga taggcgccaa
     5761 ggcggagacc gcctccttcc tcctcaagca gttcggggag gtgcggacgc tctcggacga
     5821 ggaggcggac catgtcggtc ccctcacgcc gcccgaggtc gccgacgagg ttgtcctgaa
     5881 gattggggct ccgccggtgg atctggccgc agtgctcgaa tcggtcctgg gcgcggccga
     5941 gcgcaaggga ctcgcgcatc cgcgcatcac gggtcgcgcg gggaccggcg ttaccctcgt
     6001 cgggttctcc gaggagaacg aagatggtgc cgccgggttc gtggaggaga tgcgtgagat
     6061 ctgggtccgc cgcggcggaa acgtgaccct gca
//
LOCUS       DATMJA010000345         4500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_342728, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000345 DATMJA010000000
VERSION     DATMJA010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342728"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..229)
                     /locus_tag="VNT12_08285"
     tRNA            complement(151..229)
                     /locus_tag="VNT12_08285"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Met,seq:cat)
     gene            complement(793..1923)
                     /locus_tag="VNT12_08290"
     CDS             complement(793..1923)
                     /locus_tag="VNT12_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWI46157.1"
                     /translation="MRARELGIRVGDLSPGARGAITDVEGVRVGHSTIVCGEGPLVVG
                     EGPVRTGVTVVCPREGLARDEPVFAGSHRFNGNGEMTGLEWIREAGVLATPVAITNTH
                     SVGIVRDALVRAEIRARNDEEEYWCLPVVAETYDGTLNDINGQHVTADHAFEALDSAS
                     IGPVEEGSVGGGTGMICHEFKGGIGTASRRLPEEQGGWTVGSLVQANYGSRSMLRVDG
                     LPVGRVLGTELIPSPFEDEAETQPAGTGSIIVVLATDAPLLPTQCARLAVRAGIGLGR
                     MGGGLSNGSGDIFFAFATGNRGIPRAGLSAPPATVPLQMVTNKYMTPLFHAAAEATEE
                     AILNALLAAGDMSGRDGITARGLTPDLLLGALEEVRARVPGT"
     gene            complement(1930..2703)
                     /locus_tag="VNT12_08295"
     CDS             complement(1930..2703)
                     /locus_tag="VNT12_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI46158.1"
                     /translation="MMEYLRIKNAEVPSLGLGTYRLTGEACVRAVGRALSMGYRHVDT
                     AQMYDNEAEVGRGIGDAGVDREEIFLTTKVWPSDFAHDRVIRKTRESLKKLHTEYVDL
                     LLMHWPGDGVPLGETLGAMRELQEEGSVSHVGVSNFSPSLVEEAAEHAQIFCNQVQYH
                     PYRSQDALLEQAQEMDYLLTAYTPLSRGGVQRDKILREIGEAHNKTATQAALRWLLQQ
                     DKVCAIPKATSEEHLRENLDVFDFELSDAEMGRTFSLGR"
     gene            complement(2748..3221)
                     /locus_tag="VNT12_08300"
     CDS             complement(2748..3221)
                     /locus_tag="VNT12_08300"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWI46159.1"
                     /translation="MKNPPALTRLEIRYGDLDPYGHVNNAVYLEFFEKVRICYWRALA
                     DMAGIENIEAGDVPGARYVIAETTVRYKAPIFFEDTLHGAARMRTVGNRSYAMDFVLR
                     TGESYRGGTLVAEGSAAHVFFDPQENTVQPRPDWFLPAVAKLEGRPEASFSPEDS"
     gene            3301..>4500
                     /locus_tag="VNT12_08305"
     CDS             3301..>4500
                     /locus_tag="VNT12_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI46160.1"
                     /translation="MEYEEYLEQGETLASEGLVEEALSRFEQALETAPENPEVIEAVG
                     RALLNLGRLEEAEASFLDALELDPEWVAPRMGLAMVALSRDEPFKIVHHLERAIETDS
                     GYPDAYVELGRYYGFMGEPALAKATFERWTNRVPEDADMLINAGLTLFDAADYAEALE
                     FFEKAIEAATDDVQKCGARTFRANALDMLGRYAEAVGAYEEVIAGASEWWEAHANLGI
                     CHARNGDLEKAETAFRRGLEDCPGSPEIRDELAAHLLALRKNLPEALRLSEEAVALGR
                     DEIRHLITLGEARLALGDEAGAAEAYDAVLTLDPENPDAHLELGILNERNGEMERAEE
                     HFVESLKIDPANPRALYSYASLYYTADDLEVAEEILERAIAADARYSPALSALASIQA
                     RRGEYGDA"
BASE COUNT          802 a         1459 c         1421 g          818 t
ORIGIN      
        1 gctcagttcc ctaggcatct acggcaacct cacccctctg ctctcacagt atttgctcca
       61 aaacagctta ccccagaacc cctcgctcca ctcggcctca agctcctttc tgatcgtccc
      121 aattctatcc taagcctgcc acacgaagat tggtagtgcg ggcaggattt gaacctgcga
      181 ccttcgggtc atgagcccga cgagctacca gactgctcca tcccgcgccg ccgtggaagg
      241 gaggatcgtg cgacacagca aggctgcagt ctgatcggca aggcccgcga ggagagggaa
      301 tcgggttcgc ttctaccgcc ttcgtgtggg taaaggcggt gtggagggcg ttcgggggcg
      361 ggtctggctt cgcccatgca atctggttta tgatcttgaa cctcatcgtc tgcagggcga
      421 aaccgaggct gaagatgata tggtgcgtgc cggtgaccca gatggtgcca tcaggcttga
      481 ggatgcggca cgcctccttc agccacctga cgttgaatcg gtgatccccc gcgaacccca
      541 tggacctgtc ccaggcaccc ttgtccacag gggcgagcct gccgctcttg accgtaacgc
      601 cgcccgtgga gagccggtaa ggagggtcgg cgaagaccac gtccacgctg cctgggggta
      661 tgatcttcat gagatccata cagtcagcga ggtagaccat agcccccggc tcgccgaacg
      721 tctgcaagtc ccttagctcc acaccaccat tttaggcagc ggcgtccgtc gactttagcg
      781 atggtagagc gttcaggtgc caggaacgcg ggcgcggacc tcctcgagcg cgccgagcag
      841 gagatccggc gtgaggccgc gggccgtgat accgtcgcgc ccactcatgt cgccggcagc
      901 gagaagcgcg ttcagaatgg cctcctccgt agcctccgct gcggcgtgga agagcggcgt
      961 catgtactta ttggtcacca tctgcagggg cacggtcgcc ggtggcgctg agagtccggc
     1021 gcgcgggatg ccgcggttgc ccgtggcaaa ggcgaagaag atatcgccgc tcccgttgct
     1081 gaggcctccc cccatccggc cgaggcctat accagcgcgc acagcgagcc gcgcacactg
     1141 ggtaggcagg agcggcgcgt cggtagcgag gaccacgatt atggaacccg tccccgccgg
     1201 ctgggtttcc gcctcgtcct cgaaaggcga agggatgagc tcggtaccaa gtacccgtcc
     1261 taccggtaag ccgtccacgc gcagcatgga gcggcttccg tagttcgcct gcacgagaga
     1321 gccgaccgtc cagccgcctt gctcttccgg aagccggcgc gaagccgtgc cgataccgcc
     1381 cttgaactcg tggcagatca tacccgtccc ccctccgaca gagccctcct cgacgggtcc
     1441 gatacttgcg gaatcgagcg cttcgaatgc gtgatccgcc gtgacgtgct ggccgttgat
     1501 atcgttcagg gtgccgtcgt aggtctcggc caccacgggg aggcaccagt actcctcctc
     1561 gtcgttgcgg gccctgatct cggcccttac cagggcgtcg cgcacgatac cgacgctgtg
     1621 ggtgttggtg atcgcgaccg gtgtcgccag gacgccggcc tccctgatcc actccagccc
     1681 cgtcatctca ccgttgccgt tgaacctgtg agagcccgcg aagacgggct cgtcgcgggc
     1741 aagcccctcg cggggacaga ctaccgtaac gccggtcctt actggcccct ctccgacgac
     1801 gagcggccct tcgccgcata cgatggtcga gtgccctact ctcacgccct cgacgtcggt
     1861 gatcgcaccg cgagcgcccg gcgagagatc cccgaccctt attcccagct ctcgggcgcg
     1921 cacgcggcct cagcggccca gggagaaggt acggcccatc tctgcgtcgg agagctcgaa
     1981 atcgaagacg tcgaggttct ccctcaggtg ctcctcgctg gtcgccttgg ggatggcaca
     2041 gactttgtcc tgctgcagga gccagcgcag ggccgcctgc gtcgccgtct tgttgtgcgc
     2101 ctctcctatt tcccttagga tcttatccct ttgaacgccg ccgcgggaga gtggcgtgta
     2161 ggcggtcagc aggtagtcca tctcctgcgc ttgttcgagg agtgcgtcct gactcctgta
     2221 gggatggtac tggacctggt tgcagaagat ctgggcgtgc tccgccgcct cttcgaccag
     2281 cgaaggggag aaattgctca ccccgacgtg cgatacgcta ccctcctcct gcagttctcg
     2341 catggccccg agggtctcgc cgagaggcac gccatcgcct ggccagtgca taagcaagag
     2401 gtcgacgtat tcggtgtgga gcttcttcaa gctctccctg gttttccgta tgacgcggtc
     2461 atgggcgaag tcgctgggcc agaccttggt ggtcaggaag atctcctccc tgtctacccc
     2521 cgcatctccg atgccacgtc cgacctcggc ctcgttgtcg tacatctggg ccgtgtccac
     2581 gtgcctgtag cccatggaga gggcccgccc gacggcccgt acgcacgcct ctcccgtaag
     2641 cctgtaagtg cccagtccaa gcgaagggac ctccgcgttc tttatcctca gatactccat
     2701 catgacctcc tctagagtaa ggcaatgtta ttgctctgta acacgttcta gctgtcctcc
     2761 ggggagaatg acgcttcggg gcggccttcc agcttcgcga cggcggggag aaaccagtca
     2821 ggacgtggtt gaaccgtatt ctcctgaggg tcgaagaaga cgtgggccgc agagccttcg
     2881 gcgacgaggg tgccgcccct ataggactct ccagtgcgca atacgaagtc catcgcgtag
     2941 gagcggttgc cgaccgtgcg cattcgcgcc gcgccatgga gggtgtcctc gaagaagatc
     3001 ggggctttgt agcgtacggt ggtctcggcg atcacatacc tggcgcccgg gacgtcgccc
     3061 gcttcgatgt tctcgatgcc ggccatatcc gcaagtgcgc gccagtagca gatgcgcacc
     3121 ttctcgaaga actccaggta gacggcgttg ttgacgtggc cgtaagggtc gaggtccccg
     3181 tagcggatct cgagccgcgt gagtgcgggc gggttcttca cagaggcgtt cccttcctta
     3241 caggtttccg cggacgattg tacacattca cttcacgccc catctgattt aatgagggcg
     3301 atggaatacg aggagtacct ggagcagggc gagaccctgg cgagcgaagg tctggtagag
     3361 gaggccctct cgcgcttcga gcaggccctc gagaccgcgc ccgagaaccc cgaggttatc
     3421 gaggccgtgg gacgcgctct cttgaacctg gggcggctcg aggaggccga ggccagcttt
     3481 ctcgacgccc tggagctcga ccccgaatgg gtcgcgccgc gtatggggct agcgatggtc
     3541 gcgctctcgc gcgacgaacc gttcaagatc gtgcaccacc tcgagcgggc catagagacg
     3601 gactcaggtt accccgacgc gtatgtggag ctaggccgct attacggctt tatgggggag
     3661 cccgcgctgg cgaaggcaac cttcgagcgc tggacgaacc gcgtgccaga agacgccgac
     3721 atgctcataa acgctggcct gaccctcttc gacgccgccg actacgccga agccctcgag
     3781 ttcttcgaga aggcgataga ggccgccaca gacgacgtgc agaagtgcgg cgcgcggacc
     3841 ttcagggcca acgccctgga tatgctaggc cgctacgccg aggccgtagg ggcctacgag
     3901 gaggtaatag cgggggcgtc cgagtggtgg gaggcccacg cgaaccttgg gatctgtcac
     3961 gcccgcaacg gcgatctgga gaaggccgag accgctttca ggcgaggcct cgaagactgt
     4021 cctggctcgc ccgagatcag ggacgaactg gcagcccatc tgctcgccct gaggaagaac
     4081 ctgccagagg ctctgaggct ctcagaggag gccgtggcgc tgggacggga tgagatccgg
     4141 cacctcataa ccctcgggga ggccaggctc gccctgggag acgaagccgg agccgccgag
     4201 gcctacgacg cggttttgac tctggaccct gagaacccag acgcccacct cgagctcggc
     4261 atcctcaacg agcgcaatgg ggagatggag cgggcagaag agcactttgt ggagtcactg
     4321 aagatagatc ccgcgaaccc ccgcgccctc tactcatacg cgagcctcta ctacacggcc
     4381 gacgacctgg aggtagcgga ggagatcctg gagcgggcca tagccgctga cgcgagatac
     4441 tcccccgccc tctccgcgct tgccagcatc caggcccgcc gcggcgaata cggcgacgcg
//
LOCUS       DATMJA010000346         3396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_461295, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000346 DATMJA010000000
VERSION     DATMJA010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461295"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..510)
                     /locus_tag="VNT12_08310"
     CDS             complement(145..510)
                     /locus_tag="VNT12_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46161.1"
                     /translation="MPDANELKTLASRQEQRFHEKFGYPAGEIPSIWVGQQVAVNMLG
                     GRGGDRQLAPTRASGMLEAISLDGVVLSVEERVLFIPRESILQMELYETRGRGSRLRL
                     ERETPEPADPSDPGTIGIE"
     gene            917..1528
                     /locus_tag="VNT12_08315"
     CDS             917..1528
                     /locus_tag="VNT12_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWI46162.1"
                     /translation="MKVAVVNLKGGSGKTLTSFFLATALAGRGRTLVVDADPQGSALS
                     WAAGAEEDGGTLPFAVVGLPTRDVHRRLSPVAPDYEHVVIDTPPGELAVARSALLAAE
                     VALVAVPPTPIDLDRMIPTLELVADVEDMTALSLYVLLTRVRRISREGRDARDAMREM
                     ELPLLATEIPALRFYSEAFGRAVGDTGDYENVARELLGEEVVS"
     gene            1525..1806
                     /locus_tag="VNT12_08320"
     CDS             1525..1806
                     /locus_tag="VNT12_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46163.1"
                     /translation="MTEKKGGSARRALQRGSRRAQPGGETPATGSAGTGRGGTKRITV
                     DLPRSEHKFLRDFAYDNDTDGMRVVRALLLELRDDPRLSPRVLARMGEL"
     gene            complement(2220..>3396)
                     /locus_tag="VNT12_08325"
     CDS             complement(2220..>3396)
                     /locus_tag="VNT12_08325"
                     /inference="COORDINATES: protein motif:HMM:NF021637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HWI46164.1"
                     /translation="YELLEILGKTHAGKNYDDLRDSLDRLSETSIYAENAFYSKEDED
                     FRSHRFRMWSVHFSRQKRKGRASEHHTLRFDDILVRSYNASYLKSLDSDFYYGLKRPL
                     ARSLYKLVDVKRKEALSWSVELEGLRQLLSMPESYKYPSAIKRQLEPAHRELVEKGFL
                     SRADFEERGGGATRANLVRYRVSQRFVRERSRPAPELSDGERFATDALVAQGVWPQAA
                     QELAVKHGAGHCLHYVEALPYQEGVKNPAGWLRTHIENAWPVRVPEEPPVLPTTEPNG
                     VVIDVPHLASQDSEKEATTGTQNATGGNPADYAPEAAREAQEEVHRRLASGKFDAAIE
                     AFETLPYEEYTKFIGHAVRHLDGNRYYVNLDGELYVYSGGSDPEHRHFLRRLDRYST"
BASE COUNT          605 a          974 c         1159 g          658 t
ORIGIN      
        1 tagcctagac atagagcact ctgttgattg atcaccacgg cctaacgtcg tggcacaggc
       61 tagcgaagct cggaggtcag cgaagtatat tcgccccaac catcggcttc gctggccttc
      121 tctgagttct ctgggcagag agagctactc gatgccgatg gtacccgggt ctgagggatc
      181 ggcaggctct ggggtctcac gttcgagcct cagcctgctg ccgcgcccgc gtgtttcgta
      241 gagctccatt tggaggatgg attcccttgg tataaagagg actctctcct caactgaaag
      301 gacgacgccg tcgaggctta ttgcttccag catgccgctg gccctggtgg gagcgagctg
      361 acggtccccg ccgcggccgc caagcatatt caccgcgacc tgttgtccta cccagatgct
      421 tggtatttcg ccggcgggat agccgaattt ctcgtgaaac ctttgttcct gtcttgaagc
      481 cagcgtcttg agttcgtttg cgtcaggcac agtcctccca tcggtcgttt gcgggcatct
      541 taccctttcc agaggtcttc gcgcccgtcc cgatgcccag gatcggggct acaggccctt
      601 agaagccatc ctcgcagccc agaaggcgca ggatgtaggc tagcttcggg aggacgagga
      661 aggtagggaa ggcaccttgg ttgatatccc cgactcgtcc cgctccagca gggacgggga
      721 tggcgcgccc gagagcctgc tgtagagggg acagccgacg gcggggacgc catacagaac
      781 atggagcaga aaagagctga gtactgaaat actgatgtac tgatgtacgt atcaccgtac
      841 atacgtatct ccgtacatac ttatacactg ctaaacttac gaactgaggt cagtacgaac
      901 ccagccgagg ggaacgatga aggtcgcggt agtgaacctg aagggcggca gcggcaagac
      961 gctgacgagc ttctttctgg cgacggctct ggccgggcgg ggacggaccc tggtagtgga
     1021 cgccgacccg cagggctcgg ccttgtcgtg ggcggctggt gcggaggagg acggtggcac
     1081 gctgcctttc gccgtggtcg gcttgccgac tcgggacgtt catcgcaggc tgtctcccgt
     1141 ggctcccgac tacgagcacg tggtcataga caccccgccc ggggaactcg ccgtagcccg
     1201 cagcgcgctt ttggccgctg aggtggcgct ggtggccgtg ccgccgacgc cgatagacct
     1261 cgacaggatg ataccgaccc tggagctcgt ggccgacgtg gaggatatga cagctctcag
     1321 cctctacgta ctgcttacca gggtcaggcg catctcccgg gagggcaggg acgcgcgcga
     1381 tgccatgcgg gagatggagc tgcccctctt ggccacggag attccggcgc tcaggttcta
     1441 ctccgaggcc ttcggacgtg ccgtcgggga cacgggagat tacgagaacg tggcccggga
     1501 gctgctaggc gaggaggttg tatcgtgacg gagaagaagg gcggtagcgc ccggcgtgcc
     1561 ctgcagcgcg gcagccggcg ggcgcagccg ggtggcgaga cgccggcgac cggctccgct
     1621 gggaccgggc gcgggggaac gaagcggatc acggtcgacc tgcccaggag cgagcacaag
     1681 tttcttaggg acttcgccta cgacaacgac acggacggta tgcgcgtcgt gagagcgctg
     1741 ctcctcgaac tccgagacga cccgaggcta tcgccccgcg tgctcgctag gatgggagag
     1801 ctatgagcct cggggtaggc caatccttgc ggatggaatg aggaaggtgc aatggaagcg
     1861 agactttctt ggcatgaacc tgctagcttc gctgctctcg ccgacgttgc gcgccaggaa
     1921 tccctctcgt ggccctgtga gcccctctga gggccttccc tggcttcggt agggtatccg
     1981 gatagggccg gggcttgcgg gcgattctcg cgcctctgaa gagagaccag gatcttccgc
     2041 cccaccgccc gataaccgtg cttgggccgc gctagggtac ttgcaccgta tatgtatcta
     2101 taatactaac aggagacagg gtgcaagcaa ggaccggaag gaggccaagc cagaacgcaa
     2161 ggaataaggg gaaaggtgca agaacggaga aagacaggag accagtttgt tcgctggtat
     2221 tatgttgaat aacggtcgag gcggcggagg aagtgacggt gctcgggatc cgagcccccc
     2281 gagtagacgt agagttcgcc gtcgaggttg acgtagtaac ggtttccgtc gaggtggcgg
     2341 acggcgtggc cgataaactt ggtgtactcc tcgtagggga gggtctcgaa ggcctctatg
     2401 gccgcgtcga acttgcccga agcgaggcgt ctgtgtactt cctcttgggc ctctctggcc
     2461 gcttcgggcg cataatcggc gggattacca ccggtagcgt tttgtgtccc cgtggtggcc
     2521 tctttttctg agtcctgaga ggcgaggtgg ggaacgtcta tcacgacgcc gttcggctcg
     2581 gtggtcggga ggacaggggg ctcctcgggg acgcgcacgg gccaggcgtt ctcgatatgg
     2641 gtcctcagcc agccggccgg attcttgacg ccctcctggt aggggagcgc ctcgacgtag
     2701 tggaggcagt ggccggcccc atgcttgacc gccagctcct gggcggcttg cggccacacc
     2761 ccctgggcga cgagggcgtc cgtcgcgaag cgctcgccgt ccgagagctc cggggcaggc
     2821 cttgagcgct cgcggacgaa cctttgggag acccggtagc ggaccaggtt ggctctggtg
     2881 gcgccccctc cgcgctcctc gaagtctgcg cgggagagga agcccttctc tacaagctcc
     2941 cggtgggcgg gttccagctg gcgctttatg gccgaggggt acttgtagct ctcgggcatg
     3001 gagaggagct gcctgaggcc ctccagctcc acggaccacg agagcgcctc cttgcgcttg
     3061 acgtcgacga gtttgtagag gcttcgggcc aggggccgct tgaggccgta gtagaagtcc
     3121 gagtcgaggc tcttcaggta gctggcattg taggagcgca cgaggatgtc gtcgaagcgc
     3181 agggtgtggt gctcgctcgc gcgacccttg cgcttttgcc tcgagaagtg gacgctccac
     3241 atgcggaagc gatgcgagcg aaagtcctcg tcttctttgc tatagaacgc gttttcggcg
     3301 tagatggagg tctcggagag cctgtcgagg gaatccctga ggtcgtcgta gtttttgcca
     3361 gcgtgtgtct tgcccaggat ctccagcagc tcgtag
//
LOCUS       DATMJA010000347         4390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_224693, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000347 DATMJA010000000
VERSION     DATMJA010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224693"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VNT12_08330"
     CDS             <1..281
                     /locus_tag="VNT12_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWI46165.1"
                     /translation="GKGDTVAEFDQATFGAERGEIVGPVQTEFGYHILQVTDVKPEQT
                     RSLQEVEPQIRSQLATQKQSEAFNKWIEEQKKERDVKYLKGYEPPDQG"
     gene            508..1809
                     /gene="eno"
                     /locus_tag="VNT12_08335"
     CDS             508..1809
                     /gene="eno"
                     /locus_tag="VNT12_08335"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWI46166.1"
                     /translation="MTEIIAVHGREILDSRGNPTVEVELATVSGFVGRASVPSGASTG
                     QNEAVELRDGEVERYGGKGVLNAAENVDTELAQVVVGLDAADQRTLDLAMIEADGTHN
                     KGRLGANAILGVSLAAAKASAEAAALPLYRYLGGVGAYTLPVPCANILNGGAHAANNV
                     DFQEFMVVPVGFDTYTEALHSVALVYQVLKKLLAERGLSGGIGDEGGFAPDLESNGAA
                     LGLISEAVERSGYSLGDQIAFALDPAASEFYENGEYDLAGEGKKLSREEMVDYYVGLC
                     DEYPILSIEDGLAEDDWDGWAMITEQLGDRVQLVGDDLFVTNPKILARGIGEGVGNSI
                     LIKVNQIGTLTETFETMELAHKSGYTTMISHRSGETEDATISDLAVAVNAGQIKSGAP
                     ARGERVAKYNQLLRIEEALGSSAFYPGLAAFNPRVKPGREG"
     gene            1812..3236
                     /gene="pyk"
                     /locus_tag="VNT12_08340"
     CDS             1812..3236
                     /gene="pyk"
                     /locus_tag="VNT12_08340"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWI46167.1"
                     /translation="MRRTKIVATLGPATSSEEAIGSLVRAGVDVTRFNFSHGEHSMHL
                     KNAEIVRSAARELGRNVAILQDLQGPKIRTGEVEGETELVEGSRVVIVPGDFTGDASR
                     LSTSYEKLADDVQPGHRILIDDGLIGLRVESVEDGEVVCEVLEGGPVSSHKGLNFPDS
                     TLSIRGLTPKDVEDLRFGMEEVHPDWVAMSFVRTAVEVREVKERIKEFGGNAPVISKI
                     EKHEAIDNIEEIIAASDGIMVARGDLAVELSAERVPIEQKRMVARCRRLGKPVIVATQ
                     MLDSMIRNPRPTRAEVSDVANAIFDRADAVMLSGETAVGRHPMQSVQAMERICRAAEG
                     AINYGRDIPASASWGRGDRYDAVTHAACELAEDLEAEAILTSTQTGLSCIRVARFRPP
                     NKILAISPVEHTVRMLALVWGVVAIHGEQAGSLEDRFKRAIEAAREAGHLKDGDEVIL
                     TGGTAGSVPGSTNVVEVSTVGEDI"
     gene            3258..3539
                     /locus_tag="VNT12_08345"
     CDS             3258..3539
                     /locus_tag="VNT12_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWI46168.1"
                     /translation="MSVPRRLRPLVVAIYAAFLGLLLASYVPPLQDILHNRDQIPALE
                     QKLQKTRTQNIANERLIKELQTPAGIERAARERYGMVRPGEKVYIIPEE"
     gene            3669..4121
                     /locus_tag="VNT12_08350"
     CDS             3669..4121
                     /locus_tag="VNT12_08350"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HWI46169.1"
                     /translation="MADTFKDYDAVARQLGRRPRPFRVAARCPFGLPSVIENERTREM
                     PTSFWVTCPSLVAAISRVESTGGVREAREAVGEAAVEKTHSDHRSRYGTRVAGVRDAA
                     RGGVKCLHAFTALHVSGAIPNPVAEWTLRRLESPYPEDRCCCAEDTDE"
     gene            4146..4229
                     /locus_tag="VNT12_08355"
     tRNA            4146..4229
                     /locus_tag="VNT12_08355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4180..4182,aa:Leu,seq:taa)
BASE COUNT          912 a         1295 c         1464 g          719 t
ORIGIN      
        1 tcggcaaggg cgataccgtt gccgaattcg atcaggcaac cttcggggcc gagcggggtg
       61 agatcgtcgg tcccgtccag accgagttcg gttaccacat cctccaggtt acggacgtaa
      121 agcccgagca gacccgctca cttcaggagg tcgagccgca gataaggtcc cagctcgcta
      181 cccagaagca gtccgaggcg ttcaacaagt ggatcgagga gcagaaaaaa gagcgcgacg
      241 tcaagtacct gaagggctac gagccgccgg atcaggggta ggcttcaggt gagtgcccgt
      301 gggaggagca aaacctggaa gtccgttata ttggtggcgc cgcgcgacgt ttgcgcgtag
      361 agttacgctc agaatggttg gcaagcggcc tatcgttggt aaggattggt gaagacgggt
      421 aaacatctgc gagcgccgga acggtacgcg aaaacaagac ctgacggctg aagcctgtta
      481 cccgaaacct aaaatcaggg gaggatcatg acggagatca tcgctgtcca cgggcgtgag
      541 atcctggact ccaggggaaa tccgacggtc gaggtggagc tggctacggt gagcgggttc
      601 gtcggccggg cctcggtccc ctcgggagcc tctaccggcc agaacgaggc ggtcgaactg
      661 cgtgacgggg aggtggaacg ctacggcggc aagggtgtac tgaacgccgc ggagaacgtg
      721 gacaccgagc tggcacaggt ggtcgtcggc ctcgacgctg ccgaccagag gactctagac
      781 cttgccatga tcgaagcgga cggcacccac aacaagggtc gccttggggc caacgcgatc
      841 ctcggcgtct cgctagcggc ggccaaggca tctgcggaag ccgccgcgct cccgctgtat
      901 cgttacctgg gcggagtcgg agcttacacc ctacctgtcc cttgcgccaa catcctgaac
      961 ggcggcgccc acgctgccaa caacgtggac ttccaggagt tcatggtcgt gcccgtaggt
     1021 ttcgacacct acacggaggc gctccattcg gtcgcgctgg tttaccaggt gctcaagaag
     1081 ctactcgccg agcgggggct ttcaggaggc atcggcgacg agggtggctt cgcgcccgac
     1141 ctcgagagta acggcgcggc gctcgggctc atctctgagg ccgtcgagag gagcggatac
     1201 tctttagggg atcagatagc cttcgcgctc gaccctgccg cctccgagtt ctacgagaac
     1261 ggcgagtacg atcttgccgg cgagggtaag aagctctcca gggaggagat ggtcgactac
     1321 tacgtggggc tgtgcgatga gtatcctata ctgagcatag aggacggcct cgccgaggac
     1381 gactgggatg ggtgggccat gatcaccgag cagctgggag acagggtgca gctcgtcggc
     1441 gacgacctct tcgtaacgaa cccgaagata cttgcccgtg gcatcggcga aggagtcgga
     1501 aactccatcc tcataaaggt caaccagatc ggcacgctca cggagacttt cgagacgatg
     1561 gagctcgcgc acaagtcagg ttacaccacg atgatcagcc accgcagcgg tgagaccgaa
     1621 gatgcgacga tctccgacct cgccgtggcg gtgaacgcgg gccagatcaa gtcgggcgcg
     1681 cctgcccgtg gcgagcgtgt cgcgaagtac aatcagctcc tgcgcataga ggaggccctc
     1741 ggaagctcgg ccttctaccc cggcctggcg gctttcaacc cgagggtgaa gccgggtaga
     1801 gagggctgac gttgcgcagg acgaagatag tcgcgacact gggtcctgcc acctcctctg
     1861 aggaggccat cgggtcgctt gtgcgtgccg gggtagacgt gacgaggttc aacttctccc
     1921 acggcgagca cagcatgcat ttgaagaacg ccgagatcgt acgcagtgcg gccagggagc
     1981 ttgggcgcaa cgtcgccatt ttgcaggatc tccagggacc caagatccgg accggtgaag
     2041 tggagggcga gacggagctg gtagagggca gcagggtcgt tatcgtcccc ggggacttca
     2101 cgggggacgc gagccggctc tcgacctcat acgagaagct ggccgacgac gtacagcccg
     2161 gccatcgtat cctgatagac gacggcctca tcgggttgcg ggtcgagagc gtcgaggatg
     2221 gcgaggtcgt atgcgaggtc cttgaaggcg gccctgtctc gtctcacaag gggctcaact
     2281 ttcctgactc taccctctcc ataaggggcc tcacacccaa ggacgtcgag gatctgcgat
     2341 ttgggatgga ggaggtccac cctgactggg tggccatgtc ctttgtgcgc acggcggtcg
     2401 aggtcaggga ggtcaaggag cggataaagg agtttggcgg caacgcgccg gtcatctcca
     2461 agatcgagaa gcacgaggca atagacaaca tcgaggaaat aatagcggcc tcggacggca
     2521 tcatggtcgc gcgcggggat ctcgcggtcg agctctccgc cgagagggtc cccatagagc
     2581 agaaacgcat ggtcgcgcgt tgcaggcgcc tcggcaagcc ggtcatcgtg gccacccaga
     2641 tgctcgattc tatgatccgg aacccccgtc cgacgcgcgc cgaggtctct gacgtcgcca
     2701 acgctatctt cgatcgggcc gacgcggtaa tgctctcagg ggagacggcc gtcggacgcc
     2761 acccgatgca gagcgtgcag gcgatggagc ggatctgccg ggctgcggaa ggggctataa
     2821 actacgggcg ggacatcccg gcctcggctt cctgggggag gggtgaccgc tacgacgccg
     2881 ttacccacgc ggcctgcgag ctcgcagaag acctcgaggc cgaagccatc ctgacctcga
     2941 cccagacagg gctatcttgc ataagggtcg cccgcttcag gccgcccaac aagatcctgg
     3001 ccataagccc cgtcgagcac acggtcagga tgctcgcgct ggtgtggggt gttgtcgcca
     3061 tccatgggga gcaggccggc tccctggagg accgcttcaa gcgggccatc gaggccgcca
     3121 gggaggccgg acacctcaag gacggcgacg aggtcatcct cacgggcggg acggcggggt
     3181 ccgtaccagg ctcgacgaac gtcgtcgagg tttccacagt gggcgaggac atatagaagg
     3241 agcatggtga gccccaggtg agcgtaccca ggagactgag gccgctggtg gtcgcaatct
     3301 acgcggcctt cctgggcctc ctcctcgcct cctacgtgcc cccgctccag gacatactgc
     3361 acaaccggga ccagatcccc gccctcgaac agaaactaca gaaaacacga acacaaaaca
     3421 tcgcgaacga gcgactcata aaagagctgc aaacccccgc tggcatcgag cgcgccgccc
     3481 gcgagcgtta cgggatggtc aggcccggcg agaaggtgta tatcattccg gaggaatgat
     3541 tgctatcagc gggcggcagt cagcgatcag caagtgaact atgaggctaa gcgctcaatg
     3601 atgccctgtc gctaattgtt actacttcgt gctgtggaga accgattgcg acaagaaaag
     3661 agagactgat ggctgacacc ttcaaagact acgacgccgt cgcgcggcag ctcgggcgtc
     3721 ggccgcgccc gttccgcgtc gcggctcgtt gcccgttcgg cctgccgagt gttatcgaga
     3781 acgagcggac gcgcgagatg ccgacgagct tctgggttac ctgcccgagc ctggtcgccg
     3841 ccatctcgcg cgtggagtcg accggcggag tccgggaggc acgagaagcg gtcggcgaag
     3901 cagccgtgga gaagacccac agtgaccatc gctcccgcta cgggacgcgt gtcgcaggag
     3961 tccgtgatgc agcccgcggc ggcgtcaagt gcctccacgc tttcacggcc ctacacgtct
     4021 cgggcgccat accgaacccc gttgccgaat ggaccctccg gcgcctcgag agcccctacc
     4081 cggaagaccg ctgctgctgt gcggaggaca cggatgagta gctgctatac tcccgaccga
     4141 aacccggggg cgtggcggaa tcggcagacg caccggactt aaaatccgga tcctgagaag
     4201 gagtgtgggt tcgagtccca ccgcccctac tggcgaataa cctgcaaaag agcactttta
     4261 cacagacggc agaaagcacc cggacccgat tgcagggagc tttggcacct cgtaacgttt
     4321 gtcgtaaggc tttagagacg ggagtgctgt agagtggggt tgtgacagaa ggggcgaggg
     4381 aacagtatgg
//
LOCUS       DATMJA010000348         7754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_698448, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000348 DATMJA010000000
VERSION     DATMJA010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698448"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /gene="solA"
                     /locus_tag="VNT12_08360"
     CDS             complement(<1..1074)
                     /gene="solA"
                     /locus_tag="VNT12_08360"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWI46170.1"
                     /translation="MNRTGKKAFEYIVVGCGGIGSAAVYWLSRAAGSEVLGLEQFHLG
                     HDRGGSEDHSRIIRLSYHDQAYTTLTPHTYESWSEIEDESGIQLVFETGGLDLESVED
                     EPTFINHYADAMAAAGIPYEELSADEVMERFPQFTLDESVLGVYQKEAGFVDAGKANA
                     VHIALARSRGAKVIDEAPVYGVRMAGEGVEVHTAAGTFSARKLVVAAGAWTNRVLGHL
                     GYGIPITCTQEQVTYFRTPHLREFAPDRFPVWIWHGGPDYTFYGLPVYGAVGSKAGQD
                     TGGDVVTVDTRTFIPNPRVLATLRDFLQRYIPRSLGPELYTKTCLYDMPPDREFVLGT
                     VPGVPQVAFFCGAGHAFKFAGLAG"
     gene            complement(1139..2134)
                     /locus_tag="VNT12_08365"
     CDS             complement(1139..2134)
                     /locus_tag="VNT12_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWI46171.1"
                     /translation="MSEGILQRLSEDVVLGDGGYLLELERRGYVQAGPFVPEPSITKP
                     QALAGLHREFVDAGVDVLQTLTFYASEDKLATVGLEGRVDDMNREATRIARRVAGEAE
                     RDVLVAGNLSCAWVYRPEDPKTHRRTRELFDRQLDVMIGEGVDFVIAETFNWLGEAMI
                     AAEAIKATGLPAMVTMGPDNDPNSFEGYSPGEQVKRLADAGVDIVGANCFRSPEYMLP
                     IMEEMRAAVPDSVHMAAQPVAFHGTDERPNYVSLPEFPYELEKITLPRATMGEFALRA
                     RDLGYNYIGSCCGSVAAHVREMARALGKTSSDERAWRTRTGIAQSGYELHRMKEQ"
     gene            complement(2202..3092)
                     /locus_tag="VNT12_08370"
     CDS             complement(2202..3092)
                     /locus_tag="VNT12_08370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWI46172.1"
                     /translation="MSDYGRELEFGVSVEPLADPPDWAARVTKAADRAGLDLVGIQDH
                     PYQRRFLDTWTLISTLVPVTEHIRFFPDVANLPLRPPAMLAKAAASLDVLSAGRVEMG
                     LGAGAFWDAVAGMGGPRRSPGQAVRSVEEAIKVMRLVWSEQRSLRFDGEFYSLKGMRP
                     GPHPVHDIGIWVGAYGPKMLDLIGRLADGWVPSYGHSPPGLLPEMHRRIDEGAERAGR
                     EPREIRRAYNLSGSIGAVDEGPLEGPPSRWVETLTGFALEHGMDTFIFWPSGDHERQV
                     EVFAGEVVPAVKEAVQSGRS"
     gene            complement(3089..4510)
                     /locus_tag="VNT12_08375"
     CDS             complement(3089..4510)
                     /locus_tag="VNT12_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWI46173.1"
                     /translation="MSVEEVATEKVEVPRRLLIGVLISAVFVSVLNSSMVNVVIPVIG
                     RDYGVTEAQVGWVITGYLLTYAVGIPLYGRAADVFSIRRAFIVGLIAFAVGSLICASA
                     PSLGALVFGRIVQGAGGAAIPALSSVSVTKVLPPGERGAALGLIVSSVGIGAAVGPIL
                     GGAVGALAGWHYLFVGTLILTAALIPAALYVLPDDSPEEARGFDLPGGILLGLAAGLF
                     LFGITRGQVSGFGSAGSWGSFAGSALAALGFGWRIRRSAEPFVSPTLFENRAYVSAVV
                     VGYFCMLANVSALVFIPLLVSEVNGLSPGQAGLVLTPGAVALAIISPLAGRWSDRIGV
                     RPLIFTGLVTMLLSVLSISTFGAGTTPVVVSLGMLGVGAGFALANSPNINAAASALGP
                     EEVGAGLGIFNGAFFLGGGTGPAVIGAFLAARKEADAGALNPLYALDAAAYSDAFLAL
                     TLALLISLVAALGLRSTTAKETT"
     gene            complement(4963..5895)
                     /locus_tag="VNT12_08380"
     CDS             complement(4963..5895)
                     /locus_tag="VNT12_08380"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWI46174.1"
                     /translation="MHSIFNTALPFFALIFCGYAAGRLKLLDETAAAGVNTFVFYFAL
                     PAFLFGLMSSSPIGEVVNAPFIGAYVTASLALFALTALAGLRLFEIGRAEAAVQGLAA
                     VLANTGYMGIPLISAVFGREAAVPIVVGLTLDGVLMIPLGIVIIESSKGHAEGPIHTA
                     ITTFSALTRNPLILSIFAGLAISALSLTPPEPFTNFLDLLGGTAGPCALFALGATLAG
                     RSVAGGTAEVSYMTFMKLVLHPATLFFTTTVIFSVDPLWATAALLGAALPIAANVFIV
                     ARQYNVYVARTSSAILVSTAISMVSVSALLLVLQ"
     gene            complement(5932..7107)
                     /locus_tag="VNT12_08385"
     CDS             complement(5932..7107)
                     /locus_tag="VNT12_08385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWI46175.1"
                     /translation="MPTIVDRLRADLSTGVIRKPSVNTDPLERVKRRTYLIATLAGLP
                     AVLLVWISMGPDGLVANLAFSLFVLFYLACILAIWSRVVSIRLAERVMFLGVVLFALA
                     HLSYVLFTNGSLADSRTIITEVTYTTLTVLYVVAYLIFDNRTALRISLTLFGLELFAV
                     LAKALSEVASGPNPAEILWLLRMHAFMGAVIALIYASSYLKVQLFKQREMAEVMHRLA
                     HTDPLTGVANRRELYSELRKETDESERYERPLSIIFFDLDHFKSVNDAYGHDCGDNLL
                     CEVVRSVESVLRATDRLGRWGGEEFVVLAPETDLREASRLAERLRVEIASHRYRSAPT
                     ITASLGLAQYEAGDTPETLIKRADQALYKAKILGRNRAEHANVRTGRPILTPPRTRT"
     gene            complement(7219..>7754)
                     /gene="selD"
                     /locus_tag="VNT12_08390"
     CDS             complement(7219..>7754)
                     /gene="selD"
                     /locus_tag="VNT12_08390"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWI46176.1"
                     /translation="AGDTLVLTKPLGLGILTTALKRDIVSEEEIEDAVKTASLLNKAA
                     SDAMRKIGVSAATDVTGYGLLGHLTEMLEASGVGAVVRRGSVPVWERAVPLASDGVYP
                     GGLKSNREYLEGRVTADGVDQDDLLPLYDPQTSGGLLVAVPRARASALVGALEKRGVM
                     GAVVGEVVERAGVLVTV"
BASE COUNT         1483 a         2437 c         2462 g         1372 t
ORIGIN      
        1 gccggcgaga ccggcgaact tgaatgcgtg tccagcaccg cagaagaagg cgacctgggg
       61 taccccaggc acggtgccaa gcacgaactc gcggtctggc ggcatgtcgt agagacaggt
      121 cttggtgtag agctcgggtc cgagcgagcg tgggatgtag cgctggagaa agtcgcgcag
      181 ggtggcgagg acgcgtggat tcgggatgaa cgtgcgggta tctacggtca cgacgtcgcc
      241 gccggtatcc tggccggctt tggagcctac cgcgccgtag acggggagcc cgtagaacgt
      301 gtagtcaggg ccgccgtgcc agatccagac cggaaacctg tcgggcgcga actcccgcaa
      361 gtggggcgtc cggaagtacg tcacctgctc ctgggtgcaa gtaatgggga tcccgtaccc
      421 tagatgaccg agcaccctgt tggtccacgc tccagccgcg acgacgagct ttctggcgga
      481 gaaggtccca gccgccgtgt ggacctcgac gccttcaccg gccatacgca caccgtacac
      541 cggcgcctcg tcgatgacct tcgcgcccct ggaccttgcc agggctatgt gtacggcgtt
      601 ggccttgccc gcgtccacga agccggcctc cttttgatag acgccgagca cgctctcatc
      661 gagcgtgaac tgcgggaaac gctccatcac ctcgtctgcg gagagttcct catacgggat
      721 acctgccgcc gccatcgcat cggcataatg gttgatgaaa gtgggctcgt cctccacgga
      781 ttccaggtcg aggccgcccg tttcgaagac cagctgtatc ccggactcgt cctcgatctc
      841 ggaccaggac tcgtaagtat ggggcgtcag ggtggtgtag gcctggtcgt ggtaggagag
      901 gcggatgatc cgcgagtggt cttcagaacc gcccctgtcg tggccgaggt ggaactgttc
      961 gaggccgagg acctcagagc cggccgcgcg ggagagccag tacacggcgg cactcccgat
     1021 gccgccgcaa cccacgacga tgtactcgaa tgccttcttt cctgttctgt tcacgcccgc
     1081 ctcccgccca ggctcctatg tggggcttcc cagtgggctc gacgccgctt ttcgcagcct
     1141 actgctcttt cattcggtgc agctcgtagc cggactgggc tatgcccgtc cttgtacgcc
     1201 acgcgcgttc atcggaggat gtcttgccga gggcccttgc catctccctg acgtgggccg
     1261 cgaccgagcc gcagcaggag cctatatagt tgtagccgag atcgcgggcc cgtagtgcga
     1321 actctcccat cgtggcgcgg gggagggtta tcttctccaa ctcgtaaggg aactcgggga
     1381 gcgatacgta gttgggccgc tcgtctgtcc catggaacgc tacgggttgg gccgccatgt
     1441 ggaccgaatc ggggacggcg gcgcgcatct cctccatgat ggggagcatg tactctggtg
     1501 agcgaaagca gttggcgcct acgatgtcca cgcctgcgtc cgccagacgc ttgacctgct
     1561 ctcccggcga atagccctcg aaggagttgg gatcgttgtc agggcccatg gtcaccatcg
     1621 cggggagccc tgtggccttg atggcttcgg cggcgatcat ggcctcgccg agccagttga
     1681 acgtctccgc gatgacgaag tccacgccct ccccgatcat cacgtcgagc tggcggtcga
     1741 agagctcgcg ggtacggcgg tgggtcttgg ggtcttcggg cctgtagacc caggcgcaac
     1801 tcaggttgcc agcgacgagc acgtctcttt cggcctcgcc tgctaccctg cgtgcgatcc
     1861 tggtggcctc gcggttcatg tcgtcgaccc tgccctcgag cccgaccgtg gccaacttgt
     1921 cctcgctggc atagaaagtg agcgtctgga gcacatcgac ccctgcgtcg acgaactccc
     1981 tgtgtagccc cgccagggcc tggggtttcg tgatgctggg ttcaggcacg aacggaccgg
     2041 cctgcacgta gccccggcgc tccaactcta gcaaataccc accgtcaccg aggacgacat
     2101 cctcggataa ccgctgcaga ataccctcgc tcatggacgc cctccttctc tatgtaggcc
     2161 gtgttgctta ctgtgcagcc taggaagtct atcatcgcgc ctcaggagcg ccccgactgt
     2221 acggcctcct ttacggcggg cactacctcg ccagcgaaga cctcgacctg ccgctcgtgg
     2281 tccccggagg gccagaagat gaaagtatcc ataccgtgtt ccagggcgaa ccctgtgagc
     2341 gtctcgaccc acctcgatgg cgggccttcg aggggacctt cgtctacggc gccgatgctg
     2401 cccgagaggt tgtacgcccg gcggatctcc cgtggctccc ttcctgctct ttcagcccct
     2461 tcgtcgatcc gtcggtgcat ctcagggagc agccccggcg gcgagtggcc gtacgagggc
     2521 acccagccgt ccgcgagcct ccctatgagg tcgagcatct tcggcccgta ggccccgacc
     2581 cagatcccta tgtcatgcac cgggtgcggg cctggccgca tgcccttcaa ggaatagaac
     2641 tcgccgtcga agcgcagcga acgctgttcg ctccacacta atcgcataac ctttatggct
     2701 tcttcgacgg agcgcacggc ctggcctggg ctccttcgcg gtccgcccat gccggccacg
     2761 gcgtcccaga aagctccggc gccgagcccc atctccaccc tccctgccga gagcacgtcg
     2821 aggctggcgg ccgccttcgc aagcatggcc ggcggcctga gcgggaggtt ggctacgtcg
     2881 gggaagaagc ggatgtgttc ggtcacgggc acgagcgtcg agatgagcgt ccaggtatcc
     2941 agaaaacgtc gctggtaggg atggtcttga atacccacca ggtcgagacc ggctctgtcc
     3001 gcggccttcg tgacgcgggc cgcccagtca ggcgggtccg cgagcggctc cacgctcacg
     3061 ccgaactcga gttccctgcc gtaatcgctc acgtggtctc ctttgcggtc gtgcttctca
     3121 gccccagcgc cgccaccagc gagatgagga gagccagggt aagggcgagg aaagcgtcag
     3181 agtacgcggc ggcatcgagt gcgtagagag gattcagcgc ccctgcatca gcctccttgc
     3241 gggccgcaag gaaagccccg atcacggccg gtcccgtccc gccgccgagg aagaacgcgc
     3301 cgttgaagat gccaaggcca gcgcccacct cttcgggtcc aagggcggag gcggcggcgt
     3361 tgatatttgg ggagttggcg agggcgaacc cggcgcctac gccgagcata ccgagcgaga
     3421 cgaccacggg tgtagtcccg gccccgaacg tagaaataga gagcacagaa agcagcatag
     3481 tcaccagccc ggtgaatatg agcggccgca ccccgatcct atccgaccac cgtcccgcca
     3541 gcggcgagat tatggcgagc gccaccgccc ctggcgtgag cacgagcccg gcctgccctg
     3601 gcgagagccc gttgacctcg gagacgagca acggaatgaa gacgagcgct gacacgttgg
     3661 cgagcatgca gaagtacccg acgaccacgg cggagacgta cgccctgttc tcgaagagcg
     3721 tgggggagac gaaaggctcg gcggatctcc tgatgcgcca tccgaaaccg agggcggcca
     3781 gggccgagcc ggcgaagctg ccccaggagc ccgccgagcc gaaaccggag acctgaccgc
     3841 gggtgatccc gaacaggaac agtccagctg cgagcccgag cagaatgccg ccgggcaggt
     3901 cgaagcctcg cgcctcctcg gggctgtcgt caggcaggac gtagagcgcg gccggaatca
     3961 gggcggccgt caaaatgagg gtcccgacga acagatagtg ccagcctgcc agcgcaccga
     4021 cagcgccgcc gagaatgggg cctacagcag ccccaatgcc aacactggag acgataagac
     4081 ctagcgccgc cccccgctcg ccgggaggga gtacctttgt gacggagacg ctagagagcg
     4141 ccggtatggc agccccgcca gcaccctgca caatccgccc gaagacgagc gcaccgaggc
     4201 ttggcgccga agcgcagatg aggctcccca cagcgaacgc aatgagacct actatgaaag
     4261 cgcggcggat gctgaatacg tccgcagcgc gcccatagag cgggatgccc acggcgtagg
     4321 tcagtaggta gccggtgatc acccacccga cctgagcctc tgtgacgccg tagtcccttc
     4381 ctattacagg aatcacgacg ttgaccatag agctgttgag cacagagacg aagacggcgg
     4441 agatcaaaac ccctatcagg agccgcctcg ggacctcgac cttctctgtc gctacttcct
     4501 cgacgctcaa accacggcat cctcggacgc agcaagcgcc cgcctgagcg ggccgaggtc
     4561 cgtcgagccc actcgcaacg tgcctctgat acctgcaacc ctcaaacgtc gatctccgaa
     4621 tgccaatctc gtcatctcca aaatatgctg tctaaaaaag tttgttcgtg ttcttcttta
     4681 tatcttggac cgttgccgcg cgtcattcta ccgacctgct gttcggccag ctctcgtctc
     4741 caccccaggc aaccccttta cctcacgctc gatggtctcc ctgatacttg ccatgttgcc
     4801 ttcctctctt accgactcta cttggccggc taaacaagca ataccaccga cgaggttgat
     4861 atcaagcctt gatgtgcgcc cacatcgtat gaggagggtg ggtctatcat tacaccggac
     4921 gaagggtgga atcccacctg gaccttggta cggttttccg ccctattgga ggaccaacaa
     4981 caacgccgag acagagacca tggagatcgc agtggagacg aggatggcgc tagaggtacg
     5041 ggcgacgtag acgttgtact ggcgggcgac gataaagacg ttggcggcga tgggaagggc
     5101 tgcgccgagc agggctgcgg tagcccagag ggggtcgacg ctgaagatta cggtggtcgt
     5161 gaaaaagagg gtagccgggt ggagtacgag cttcatgaac gtcatatagg agacttcagc
     5221 tgtgccgccc gccacggagc gtcctgcgag ggttgcgccg agggcgaaca gggcacaggg
     5281 gcctgctgtg ccgccgagca gatcgaggaa attggtgaaa ggttcgggcg gtgtcaggct
     5341 taaagctgag atggccaggc cggcgaagat ggagaggatc agggggtttc gggtaagggc
     5401 cgagaacgtg gtgattgcgg tgtggatggg gccttctgcg tggcctttgg aggactcgat
     5461 gatcacgatg ccgaggggga tcatcagcac gccgtcgagg gtgagcccga cgacgatggg
     5521 gacggcggcc tccctgccga agacggcgga gatcaagggt atacccatat agcccgtgtt
     5581 cgccagcacc gccgcgagtc cctgcaccgc cgcctcggcg cgtcctatct cgaagagccg
     5641 gagtccggcg agcgcagtca gcgcgaagag ggcgaggctc gccgtcacgt acgcccctat
     5701 aaacggcgcg ttgacgacct ctcctattgg cgaggaggac atcaggccga acaggaaggc
     5761 cggcagggcg aagtagaaga cgaacgtatt tacccctgct gctgcggtct cgtccaagag
     5821 cttcagacgt cccgcggcgt agccgcagaa gatcagggcg aagaaaggca gcgcggtgtt
     5881 gaatatagag tgcaaggccc gctaatctta cacggacggt tcgaaccttc gttacgtccg
     5941 ggttcgaggc ggggtgagga ttggtctacc cgtccttacg ttcgcatgct ccgcgcggtt
     6001 gcggccgagg atcttggcct tgtagagggc ctggtcggcg cgtttgatca gcgtctcggg
     6061 ggtatccccc gcctcatact gtgcgagacc aaggctcgcc gttatggtgg gtgcggagcg
     6121 gtaacggtgg ctggcgatct ccaccctgag gcgctctgcc agccggctcg cttctcgaag
     6181 gtcggtctcg ggtgcgagga cgacgaactc ttccccgccc cagcggccta gccggtcggt
     6241 cgcgcggagt acggattcca cagatcttac gacctcgcac aatagattgt ccccacaatc
     6301 gtgtccgtag gcatcgttca cgctcttgaa gtggtccaga tcgaagaaaa tgatggagag
     6361 cggtctctcg tagcgttccg actcgtccgt ctccttgcgc aactcggaat agagctcgcg
     6421 gcggttggcc actcccgtca acggatctgt gtgggccagc cggtgcatga cctcggccat
     6481 ctccctctgc ttgaacaact ggactttgag gtacgacgag gcgtagatca gggcgatcac
     6541 ggctcccatg aaagcgtgca ttctaagcaa ccaaaggatc tctgcagggt tcggtccaga
     6601 agcgacttcc gagagagctt tggcgaggac cgcgaacagt tccaggccga aaagcgtaag
     6661 tgatatccgc agggccgtac ggttgtcgaa gatcaggtaa gccaccacgt aaaggacggt
     6721 caaggtggtg taagtgacct ctgtgatgat ggtacgggag tcggccagac taccgtttgt
     6781 gaagagaacg taggacagat gggcaagcgc gaacaggacg acgcccagga acatcacacg
     6841 ctcggccagc cgtatcgaaa cgaccctact ccatattgcc agaatgcagg ccaggtagaa
     6901 gagtacgaac agcgagaagg cgaggttggc gacgaggcca tcggggccca tggagatcca
     6961 gaccaggagt acggcgggca ggccggcgag ggtggctatc aggtaggtcc ttcgctttac
     7021 ccgctcgaga ggatccgtgt tcacggaagg tttacggatg acgcccgtgg acagatcagc
     7081 ccgcaaccga tctacaatcg tcggcatctt tgaatccacg ctcctcatgc ttgcagatag
     7141 aggataatgc ttcggcctga ccgttgcata caccaaatga gctattttgg ctacaccggc
     7201 gagacatttg tcttcgcact acacagtcac cagcaccccg gcgcgctcaa ccacctcgcc
     7261 gacgacagcc cccattacgc cacgcttctc taacgcgccg accagcgcgg aggcccgagc
     7321 ccttggcaca gcgacgagca accctccgct cgtctgcggg tcgtagagcg ggagtaggtc
     7381 gtcctggtct acgccgtccg ccgttacacg gccctccagg tactctctgt tgctcttcag
     7441 gccgccaggg tagacgccgt cgctcgcaag cggcacggcc cgctcccaga caggaacgct
     7501 gcctcgacgt acgaccgccc cgacgccgct cgcctccagc atctccgtca ggtggccgag
     7561 gagaccgtag cccgtcacgt ccgtggccgc ggagacgccg atcttgcgca tcgcatcgct
     7621 ggcggccttg ttgaggaggg aggctgtctt tacggcatct tctatctcct cctccgagac
     7681 gatgtctctc ttgagcgccg tggtcaggat gccgagaccg agcggtttgg tcagaaccag
     7741 ggtgtctcca gctt
//
LOCUS       DATMJA010000349         4315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_876766, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000349 DATMJA010000000
VERSION     DATMJA010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876766"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..521)
                     /locus_tag="VNT12_08395"
     CDS             complement(99..521)
                     /locus_tag="VNT12_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWI46177.1"
                     /translation="MHANPDLREIRDLLQRSGTVAVVGLSDRPSRTSHAIAGALQGFG
                     FKIYPVNPNLRGPVLGEEPYETVQNIPTPVDIVDVFRRSGKVMPVAQDAVAAGAKVLW
                     MQSGVINEEAAAYAEEHGLTVVMDRCIKVDYATLVGVS"
     gene            complement(767..1534)
                     /locus_tag="VNT12_08400"
     CDS             complement(767..1534)
                     /locus_tag="VNT12_08400"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWI46178.1"
                     /translation="MSEMNNERFEDLKDAYVLGALPEEERLSFEDYLAAHPERQAEVD
                     ELGAVAGLLAFSPQEQEPSPELRNRIMEVVEAEAAPRRDTRRSVSARIGEYLSVRSLA
                     LGAAALLVIGLLSWNVMLQGQVEDLQGRVQDAQGQIQDLQGELEIAQAQQSQTIKLKG
                     SWAEQGAIAEVASMQKHQIILVARNMPSVPEDKTCQIWVIKGDVPKASGLFQPDGNGT
                     ATPITNSIRKADAIAVTVERAGGSKKPTSTPVLSAKL"
     gene            complement(1531..2118)
                     /locus_tag="VNT12_08405"
     CDS             complement(1531..2118)
                     /locus_tag="VNT12_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI46179.1"
                     /translation="MAERGRKYLILADEDLISLVEAADAEAFATLYDRHSRAAFSLAY
                     RMMGERQASEDLAQDAFLKVWRGASSYRADRGSVRTWILSIVHNRGIDQIRSRASRRR
                     TEERIEASAPRSQPSEAFAETLRNSQRDQVREALDTLPPEQLKILELAYFSGYTHMEI
                     SDLLDLPLGTVKGRMRLGLKKIRDYFESRDAAVPR"
     gene            2240..3064
                     /locus_tag="VNT12_08410"
     CDS             2240..3064
                     /locus_tag="VNT12_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI46180.1"
                     /translation="MRLSVLSSGSSGNATYMETDSGGLLVDAGLPPRRIGALLASIGR
                     SLADVNAVLLTHGHTDHTCGVPALVRERNIEIYAAPGVGESLGANTVAPGEPCSVEDL
                     GVMFFEVPHDSMTYGVRVSDGSNAVAMATDLGEAGIDVLRSMSGAEAVVLESNHDPDW
                     LSRGPYSARLKRRIRSRHGHLSNRQAAAAAVALAPHGLKDLVLAHLSKTNNSPTRACG
                     TVDRALREAGHGSVRVRAAIAGLPTPWIEVGAPLLVPEHVYRYGEQGAAGQLFGIE"
     gene            3061..3534
                     /locus_tag="VNT12_08415"
     CDS             3061..3534
                     /locus_tag="VNT12_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWI46181.1"
                     /translation="MIRRCTLVSVGKTKGWAAEASKDYASRLRRYFPVNIIEVAEEDI
                     NRRSHKEVLAAEGERILKRLPKDAYVISLDREKGKQRPSEKLALKLEDLGASGRSHVA
                     FVIGGPLGLSPDVLQRSNDIWSFGSITLPHSLARVVLLEQLYRAAKIHRNEKYHW"
     gene            3577..3816
                     /locus_tag="VNT12_08420"
     CDS             3577..3816
                     /locus_tag="VNT12_08420"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HWI46182.1"
                     /translation="MLTYTYMRVQIDRFEDNGWAVLLPYPDGRTTFDVPRELLPEEVS
                     AGDVFDVRFEYDREETLRIAEENRRLLDELLGGEE"
     gene            3813..>4315
                     /gene="argS"
                     /locus_tag="VNT12_08425"
     CDS             3813..>4315
                     /gene="argS"
                     /locus_tag="VNT12_08425"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWI46183.1"
                     /translation="MSFEERLASAVRQAVYRTYGVELDGIHVERPNDPAHGDFATNVA
                     LANARVLRRNPREVAENLVDALDAPFVREAEVAGPGFVNFRLSPEALWDEVASLLREG
                     DRYGRREPSGDPVLLEFVSVNPTGPMHVGHGRQAAYGDSLARIMEAAGRNVSREYYFN
                     DGGNQIRL"
BASE COUNT          804 a         1386 c         1294 g          831 t
ORIGIN      
        1 taaccagcgg cgaaccatca cccaaacgag cccctctcag cgcacccctc ggatcagtcg
       61 aaccatcaaa gtagcccatc ggcgtgctga catgctgcct agctgacacc tacaagcgtc
      121 gcgtagtcta cctttataca gcggtccatg acgacggtca ggccgtgctc ttcagcgtag
      181 gcggcggcct cttcgttgat cacgccggat tgcatccaaa gtaccttggc gccggcggcc
      241 acggcgtctt gggccaccgg catgaccttc ccgctacgcc ggaacacgtc gactatgtcc
      301 accggcgtgg ggatgttctg gaccgtctcg taaggctctt cgccaagtac aggaccacga
      361 agattcgggt tgacggggta gatcttgaat ccgaagcctt gcagtgcgcc ggcgatcgcg
      421 tggctggtgc gggagggcct gtccgaaagt ccgacgacag ccacggtgcc cgagcgttgc
      481 agaagatccc ttatctctcg aaggtcaggg tttgcgtgca tgtcactctc ttcctcaggg
      541 acctcaggtc ccgatgttat acgatctcca cgcccatcgg cgcaagggcc tgctctttgc
      601 ccttggcctc gaccctcggc tacggtccag acgcgctcgt cgttctccag cgcgaggtag
      661 cgcaggatcc gcgcctcggt acgccataga caggccagaa agctgacatg ctgccagtgc
      721 tcaccagcgc gggatgcggg aattgctggc aattcccgca tcccacttac agtttggccg
      781 aaagtacggg ggtgctcgtc ggcttcttgg agcccccggc ccgctcgacg gtcaccgcta
      841 tcgcgtcggc cttccttatg gagttcgtga tcggcgtggc cgtcccgttc ccgtcgggtt
      901 ggaagagtcc gctggctttg ggcacgtcgc cttttatgac ccagatctgg cacgtcttgt
      961 cctcaggtac cgaaggcata ttcctggcca caaggatgat ctggtgcttt tgcattgagg
     1021 ccacttcggc aatggcaccc tgctctgccc acgatccctt gagcttgatc gtctggcttt
     1081 gctgggcctg cgcgatttcg agctcgcctt gcagatcctg tatctggccc tgcgcgtcct
     1141 gaacccggcc ttgtaggtcc tctacctggc cttgcaacat cacgttccac gatagcaacc
     1201 cgatcacgag cagggccgca gcgccgagcg cgagactccg gacgctgaga tactccccaa
     1261 ttcttgcaga cacggaccgt cgcgtgtcgc ggcggggcgc cgcctcggcc tcgactacct
     1321 ccatgatcct gttgcgcagc tccggcgatg gttcttgttc ttgcggggag aacgcgagca
     1381 ggcccgctac ggcgccaagc tcatcaacct cggcttgccg ttcggggtgg gcggcgaggt
     1441 aatcttcgaa actcagcctt tcctcctcgg gcaaagcgcc gaggacgtag gcgtctttga
     1501 gatcttcgaa gcgttcgttg ttcatctcac tcatctcggc accgccgcgt ctctagactc
     1561 gaagtagtcc ctgatctttt tcagtcccaa acgcatcctg ccctttaccg ttccgagagg
     1621 caggtcgaga aggtcagata tctccatgtg tgtgtacccg gaaaagtacg ccagctccag
     1681 tatcttgagc tgctcgggcg ggagggtgtc aagcgcctcc ctgacctggt cgcgctgcga
     1741 gttcctcaag gtctccgcga aggcctcgct cggttgggag cgtggggctg aggcttcgat
     1801 cctttcctcc gtcctgcgcc ggctcgcccg ggaacggatc tggtctatgc cgcggttgtg
     1861 cactatggag aggatccagg tccgcacgct gccgcgatct gcacgatagc tgctagcgcc
     1921 ccgccagact ttcaggaacg catcttgcgc caggtcttcg gaggcttgac gttcccccat
     1981 catcctgtag gccagggaga aggctgcgcg gctgtggcgg tcatacagcg tcgcaaacgc
     2041 ctcggcgtca gccgcctcga ccagggagat caaatcctcg tcagcaagaa tcaggtactt
     2101 gcgtcccctt tcagccatac cctgattcta cggcaatacg cccagcccgg atcaacgccc
     2161 ccgtgccaat tcgggcgtgc taagatctaa acgggtctcg ttcccgctga tcaaacgccc
     2221 aacctgatag aggacggatt tgcggctgag tgttctttcg agcggaagct cgggcaatgc
     2281 gacctacatg gagacggatt caggcggcct cctcgtcgac gccggcctcc cgccacggcg
     2341 cataggtgcc ctcctcgcca gtatcggcag gagcctggcc gacgtaaacg ccgtcctgct
     2401 gacccacgga cacaccgacc acacttgcgg cgtccctgct ctggtgcgcg aacgcaatat
     2461 cgaaatctac gccgcgcctg gtgtcggtga gtctctcggc gcgaacacgg tagccccagg
     2521 agaaccctgc tccgtcgaag accttggagt gatgttcttc gaggtcccgc atgactcaat
     2581 gacgtacggg gttagggtat ccgacggcag taatgccgtg gcgatggcga cggatctcgg
     2641 ggaggctgga atcgatgtac tgcggagcat gagtggggcc gaggcggtgg tcctcgagtc
     2701 gaaccacgat ccagactggc tcagtcgggg cccatactcg gcgagactga agcgccggat
     2761 ccgctcacgg cacggccacc tctcgaaccg gcaagcggca gccgccgccg tggcgctggc
     2821 tccgcacggg ctcaaggacc tcgtccttgc ccacctctcc aagaccaaca actctccgac
     2881 ccgggcctgt ggcaccgtag accgggcgct tagggaagca ggccacggct ccgtcagggt
     2941 tcgggcggcg atcgccggcc ttccaacacc ctggatcgag gtaggagccc cgctgctggt
     3001 ccccgaacac gtctaccgct acggcgagca aggcgcagca ggtcagctct tcggtatcga
     3061 gtgatacgcc gctgcaccct cgtctcagtc gggaaaacaa aaggctgggc cgccgaagcc
     3121 tcgaaagact acgcaagtcg cctgcgccgc tatttccctg taaacatcat cgaggtcgcc
     3181 gaggaagaca taaaccgccg ctcccacaaa gaagtgctcg ccgcggaagg agagcgtatc
     3241 ctcaagcgcc tcccaaaaga tgcgtacgtg atctccctcg acagggagaa aggaaagcag
     3301 cgcccatcag agaaactcgc cctgaaactg gaagacctcg gcgcctcagg ccggagtcac
     3361 gtcgcctttg tgatcggcgg cccgctcggc ctctcccctg atgtactgca aagatccaac
     3421 gacatctggt ccttcggcag catcacactc ccgcactccc tcgccagggt cgtcctcctc
     3481 gaacaactct accgcgcagc aaaaatacac cgcaacgaga aatatcactg gtagctccgc
     3541 ttcgcaggtc agcacgctcc ggctgccgaa ggcagcgtgc taacatacac atatatgcgt
     3601 gtgcaaatag acaggttcga ggacaatggt tgggccgtgc tcttgcccta ccctgacggg
     3661 cgtacgacct tcgacgtgcc gcgcgagttg ctacctgaag aggtctccgc gggggatgtt
     3721 ttcgacgtcc gcttcgagta cgacagggag gagaccctgc ggatcgccga ggagaaccgg
     3781 cggcttttgg acgagcttct cgggggtgaa gaatgagctt cgaggagcgt ctggcgagcg
     3841 ccgtgcggca ggccgtctac aggacctatg gggtcgagct ggacggtatc cacgtcgagc
     3901 ggccgaacga ccccgcccac ggcgacttcg cgaccaacgt ggcactcgcc aatgcaaggg
     3961 tattgcggcg gaacccgagg gaggttgctg agaacctcgt ggacgccttg gacgccccgt
     4021 tcgtgcgtga ggccgaagtc gccggaccag gcttcgtcaa cttcaggctg tctcccgaag
     4081 cgctctggga tgaggttgcc tctctgctcc gtgagggtga ccggtacgga aggcgagagc
     4141 cttcgggaga tcccgtactg ctcgagttcg taagcgtcaa cccgaccggg cctatgcatg
     4201 ttgggcacgg acgtcaggca gcctacgggg actcattggc caggatcatg gaggctgccg
     4261 gcaggaacgt ctcaagggag tactacttca acgacggagg gaaccagatc cgact
//
LOCUS       DATMJA010000350        10243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_876968, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000350 DATMJA010000000
VERSION     DATMJA010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876968"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /gene="gltX"
                     /locus_tag="VNT12_08430"
     CDS             complement(<1..1456)
                     /gene="gltX"
                     /locus_tag="VNT12_08430"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWI46184.1"
                     /translation="MENDIKIRVRYAPSPTGRLHVGGVRTALFNWLFARKNRGTFVLR
                     IEDTDLERSTEESVEQLGRSMRWIGLDWDEGPEAGGPHPPYRQSERFDLYREAARKLL
                     DSGAAYYDFTTPEDLAGLRERARVEGRQPIYTGGEYREMDPDEAGEKIAAGEPYTVRF
                     KTPREGQTVVQDMIRGPVTFENANLEDFVLMKSTDTPTYNFAAAFDDAEMEISHVIRG
                     DDHLSNTPRQILIHEALGNPLPAFAHVPQVLGPDKKKLSKRHGAASVEDFAERGYLPE
                     ALFNYLALLGAGYAADEEIFTPEELAERFRIDRVSGNPAILDEKKLRSVNAVYLRRRS
                     PEELATLAAPMLIESGAATEEELDRDPARLEDIMDLLKERIALTTEIPDSVGYFYGGS
                     LDYDEAEFEKQFGKEFVRENFPELVERLTALPEWTETAIEEVVRGLAAEKEKGARHLI
                     HPLRFAVTGRTVSAGLFETMQLLGRERSLLRAGDA"
     gene            complement(1569..3173)
                     /locus_tag="VNT12_08435"
     CDS             complement(1569..3173)
                     /locus_tag="VNT12_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HWI46185.1"
                     /translation="MTGHLELWVLGLLAAVAGLVMLSNRLGVPYPVFLVLGGLALGLV
                     PIVPEIGLPPDLVLLIFLPPLLYSAAFFSSPRDLKANLRPIASLSIGLVLLTTITVAL
                     VAHLLVGLPWAAAFVLGAIVSPTDPVAATAIAGRLGAPRRIVTILEGESLINDGTALV
                     LYSVAVSAVVTGTFSLPHAVLEFVLYGVGGTAIGLVVGWVITRVRRWIGDPLVEITVT
                     LLTPYAAYIPAEELGVSGVLATVATGLYLGWRNPETTAPRNRLQLFGLWDVLPFLLNS
                     VLFILIGLQLPNILESISGEYSMMSVVLYAALVSLAVIGTRLLWTFPATYLPRYLSRS
                     IRERNPSPPWQQVAAVAYTGMRGAISLAAALAIPLTVQNGASFPGRDQIIFLTFCVIL
                     VTLVVQGMSLPLLIRRLGLAGAEGEEEREETEARLRAAEAALARLDELADEARVREDT
                     TTRMHDLYEYRRRRFAARHSEQTESGEEDDGYEERSLAYQRFRRELLGAERAELLRLR
                     SEGRISDEVRRRVERDLDLEEARLEI"
     gene            complement(3240..3713)
                     /locus_tag="VNT12_08440"
     CDS             complement(3240..3713)
                     /locus_tag="VNT12_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46186.1"
                     /translation="MSTSMGDIAANVGKGLFAGVAGTAVMTVSSTLEMKLSGRGASQT
                     PAEAAEKVLKVEPEDEGAEARFSNLVHWGYGTGWGAVRGLLASAGLCGPVATAAHLGL
                     VWGAEQVVLPALDVSAPVFKYGSKATATDLLHHVVYATATGLAYSYLDRSQSRPG"
     gene            complement(4114..4770)
                     /locus_tag="VNT12_08445"
     CDS             complement(4114..4770)
                     /locus_tag="VNT12_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HWI46187.1"
                     /translation="MATARETILWRIRHATRDVPEAERPEDVSVEREYRKEDGAEREE
                     IVARFAERTAEYEADVRRVGHDVLPRAIEEALKRRGVRRLVIPPYLPREWIPEGIEIL
                     RDAARSRLTNQELDESDGVLTGCALGIAQTGTIVLDAGRAQGRRTLTLLPDYHLCVVR
                     EQQIVGLVPEAFASLERRVKGEGRAITFISGPSATSDIELDRVEGVHGPRSLEVLIAG
                     "
     gene            complement(4770..6233)
                     /locus_tag="VNT12_08450"
     CDS             complement(4770..6233)
                     /locus_tag="VNT12_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HWI46188.1"
                     /translation="METKKSDAFSPGPTPTFEESAKESLSDSQLRRNLGKATQTIRHK
                     RALAVEEMPDWEGLREAGRALKERVLRHLDEYLLQLEESVTRAGGEVHWARDAEEANR
                     IISGIVREGGAEEVVKVKSIATDETKLNEHLEREGIKAFETDLAELIIQLAGETSSHI
                     LVPAIHKNRAEIRELFMEELGAQDLSDRPSDLTEAARLYLREKFLKAKIGISGANFAV
                     AETGTICVVESEGNGRMCTTLPPVLVTLMGIEKVIPTWRDFEVFMQLLPRSSTAERMN
                     PYTSFWTGVSGDAGDGPREFHLVLMDNGRTDVLADEIGRQALNCIRCSACLNVCPVYE
                     RTGGHAYNSVYPGPIGAILTPQLVGVGDPGSDSLPYASSLCGACYQVCPVKINIPEVL
                     VHLRGRVVRHKQYMGGPRSKLDPENVAMQTVAKLFSDPQLYEGAQRLARAGQWPFARG
                     GSISRLPGRLAGWTAVRDLKPIPQQTFREWWRNRRSV"
     gene            complement(6233..7099)
                     /locus_tag="VNT12_08455"
     CDS             complement(6233..7099)
                     /locus_tag="VNT12_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWI46189.1"
                     /translation="MSPTVTPLLVRRERSLREGNEGRRALEWSIMVLLDAEEKGTIAV
                     SVALFITCFNDTLFPETGQATVEVLERLGVEVDFPYAQTCCGQMHFNTGYQREAIPLV
                     RRFVEIFGGYEAVVAPSGSCVSMVRELYPMAADLAGDGRLTEQVEELGPKVFELSEFL
                     VRKLGVSDVGAYYPHRVTYHPTCHSLRMLRVGDAPLELLRNVRGIDLVELSGAEECCG
                     FGGTFAVKNADTSAAMLSDKIRRVLDTDAEVCTAGDNSCLMHIGGGLARLRTGVETVH
                     LAQILASKAEEG"
     gene            7284..8000
                     /locus_tag="VNT12_08460"
     CDS             7284..8000
                     /locus_tag="VNT12_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWI46190.1"
                     /translation="MKGGRIVGVRGRSVDRVNKGRLDPKDLFGWQANNSEDRLTRPLV
                     RKDGELVEASWDEAFDLIVSRSKALLEEKRPLAFGFYTTGQLFLEEYYTLGVIGKAGL
                     GTPHMDGNTRLCTATAAQALKETFGTDGQPGSYADIDHADTILHFGHNLAETQAVTWM
                     RVLDRLAGPNSPKLVVVDPRPTPVAKRVTVHLPIKSGTNVALLNAFLHEILENGLYDQ
                     EYVEAHTKAHPIREEIIHLG"
     gene            8389..9078
                     /locus_tag="VNT12_08465"
     CDS             8389..9078
                     /locus_tag="VNT12_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46191.1"
                     /translation="MEEKLMPDFDTRKPQGSNEPNRPRIALIANRLSRLLSASKVRIL
                     SIASTLRRFLMANRLRTLLIGGGMMLFVAAAALGLLIYSSIELADGGQKVTDLQQKIE
                     GATLTPLEGCPVGGDINQSKIRDMVHGFSSTKKIVFVRGSDITYLYYLGVGGTIDIVA
                     NIELYMISTDGTSLTRLTKTTAKEYGANRLPDGRRMNILWDTDGRTIRGISYFDSEPG
                     TTLSGRPPIPT"
     gene            complement(9784..>10243)
                     /locus_tag="VNT12_08470"
     CDS             complement(9784..>10243)
                     /locus_tag="VNT12_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46192.1"
                     /translation="RIGPPAPGPDTRLTETTPAMVRLAASRPVAKEARRSLGLQAGSA
                     ELLDNLEVSRVRGTTFIDLSYRGTDRKKAAQTVNALASVASERLSEAPGKNLTAIVWE
                     EAQVPPAVPEPKPLRNGLLTLVGVWALYAGLTLAMLVRLEAVRAVGGEGT"
BASE COUNT         1848 a         3251 c         3211 g         1933 t
ORIGIN      
        1 tggcgtcccc ggcgcggagc aggctgcgtt cgcgtcccag gagctgcatg gtctcgaaga
       61 gacccgcgct gacggtgcgg cctgtaacgg cgaagcgcag ggggtgtatc aggtggcgcg
      121 cgcccttctc cttttcggcg gcgaggccac gcaccacttc ctcgatcgcg gtctccgtcc
      181 actcgggaag agccgtgagg cgttcgacga gctcggggaa gttctcgcgg acgaactcct
      241 tgccgaactg cttctcgaac tcggcctcat cgtagtcgag ggagccgccg tagaagtagc
      301 cgacggagtc ggggatctcg gtcgtcaggg cgatgcgctc cttgaggagg tccatgatgt
      361 cctcgaggcg cgccgggtcc cgatctagct cctcctccgt cgccgcgcca gactcgatga
      421 gcatgggggc ggcgagagtg gcgagttcct ccgggctcct gcgcctgagg tagacggcgt
      481 tcacggacct tagcttcttc tcgtcgagaa tcgcagggtt gccgctcaca cggtcgatgc
      541 ggaagcgctc tgctagctcc tcgggggtga agatctcctc gtcagcggcg tagccggctc
      601 cgaggagcgc gaggtagttg aagagcgcct cgggcaggta gccccgctcc gcgaagtcct
      661 cgacgctcgc cgcgccgtgg cgcttgctga gcttcttctt gtctggtccg aggacctggg
      721 ggacgtgggc gaaggcagga agcgggttgc ccagggcctc gtggatcagg atctggcgcg
      781 gagtgttgga aaggtggtcg tcgccgcgga tgacgtggct tatctccatc tctgcgtcgt
      841 cgaaggcggc agcgaagttg taggtcgggg tgtcggtgga cttcatcagg acaaagtctt
      901 ccaggttggc gttctcgaac gtgacgggac cacggatcat gtcttgcacc actgtctgac
      961 cctcgcgcgg ggtcttgaag cggacggtat agggctcgcc cgccgcaatt ttctcccccg
     1021 cctcatctgg gtccatctcg cgatactcgc cgccggtgta gatcggctgc cgtccttcga
     1081 cccgcgccct ttcacgaagc ccggcgaggt cctcaggcgt tgtgaagtcg tagtaggctg
     1141 cgcccgagtc gaggagcttc cttgcggcct cccggtagag gtcgaagcgc tcggactggc
     1201 ggtagggggg atgggggccg ccggcttcgg ggccctcgtc ccagtccagg ccgatccaac
     1261 gcatcgaccg cccgagctgc tcgaccgact cctccgtgga acgctccaga tccgtgtcct
     1321 ctatccgaag cacgaacgtg ccgcggttct tgcgcgcgaa gagccagttg aagagcgcgg
     1381 tccgcacacc gccgacgtgc agtcgccctg tcgggctagg cgcgtacctg accctgatct
     1441 ttatgtcgtt ctccatgagc taaacctaac acacgttggt cgaggccgtg cagcattaac
     1501 ggtctgctac ccggttgtgc gccttcaatt ggtcccgggg cagaacgccg aggttatccg
     1561 ggccgcggct atatctccag ccgcgcttct tcgaggtcga gatcccgctc gacgcgtcgt
     1621 ctcacctcgt cgctgatacg tccttcgctc ctgagccgga ggagctccgc acgctcggcg
     1681 cccagcagct cccgcctgaa acgttggtat gccagagagc gttcctcgta gccgtcgtcc
     1741 tcttcaccgc tctcggtttg ctcgctgtgg cgggcggcga agcgccggcg acggtattcg
     1801 tagagatcgt gcatccgggt ggtcgtgtcc tcccgcaccc gagcttcgtc cgcgagttca
     1861 tcgagcctcg cgagcgccgc ctcagccgcc cgcagccgcg cctctgtctc ctcgcgctcc
     1921 tcctcgcctt ctgctcctgc caggcccagg cggcggatga gcagcggcag gctcatcccc
     1981 tgtaccacga gggtgacgag gatgacgcag aaggtcagaa agatgatctg gtcgcgcccg
     2041 gggaacgagg cgccgttctg tacggtgagg gggatggcca gcgcggctgc cagtgagatg
     2101 gcccccctca tgcccgtgta ggcgacggcg gcgacttgtt gccagggagg agaggggttt
     2161 cgctcgcgta tggaccggct caagtagcgg ggcaggtagg tagccgggaa cgtccagagc
     2221 agccgcgtcc cgatcaccgc caggcttacg agcgcggcgt acagcaccac cgacatcatc
     2281 gagtactcgc cggagatact ctcgaggatg ttgggaagct gcaacccgat gaggatgaac
     2341 agcaccgagt tgagcaagaa tggcagcacg tcccataacc cgaacaactg caggcggttg
     2401 cgcggggcag tcgtctcagg attgcgccag ccgaggtaga gtccggtcgc gaccgtggcg
     2461 agcacgccgg agacgccgag ctcttcggcc ggaatgtagg cggcgtaggg cgtgaggagc
     2521 gtgacggtaa tctccacgag cgggtccccg atccagcgcc gcacgcgcgt gatcacccac
     2581 cccacaacga gccctatcgc cgttccaccg accccgtaga gcacgaactc gagcaccgca
     2641 tggggcagcg agaaggttcc cgttacgacg gccgacacgg ccacgctgta gagaaccagc
     2701 gccgtcccgt cgttgatgag gctctcgccc tcgaggatcg tcacgatccg gcgcggcgcg
     2761 cccaggcgcc ctgcgatggc cgtcgccgcc accgggtcgg tcggcgagac gatggccccc
     2821 agaacgaacg ccgccgccca cggaaggccg accagtaggt gcgccaccaa ggcgacggtg
     2881 atggtagtga gcagtacgag gccgatggac agggacgcta tcggtctcag gtttgccttg
     2941 aggtcgcgtg gggaggagaa aaaggccgcc gagtagagga gcggcgggag gaagatcaga
     3001 aggaccagat ccggcggtag cccgatctcg ggcacgatgg gaacaaggcc caacgctagt
     3061 cccccgagga cgaggaagac cgggtaaggc acccccaggc ggttggagag catcaccagt
     3121 ccggccacgg ccgcgagcag cccgaggacc cagagttcca gatgccccgt catcgcgccg
     3181 tctcgccgga actacgtctc atcgtaaaaa agccctccag cctgcgacga atcgcctcct
     3241 caacccgggc gggactggct cctgtccagg taggagtagg cgagacctgt ggccgtggcg
     3301 tagacgacgt ggtgcaacag gtcggtcgcc gtcgccttcg agccgtactt gaacaccggg
     3361 gccgagacgt caagggccgg cagcacgacc tgctctgctc cccacaccag cccgaggtgc
     3421 gccgcagtgg caacagggcc gcagagtccg gccgaggcca gaagtccccg cactgcgccc
     3481 cagcccgtac cgtatcccca gtggaccagg ttggagaacc gggcctcggc tccttcgtcc
     3541 tcgggctcga ctttaaggac cttctcggcg gcctcggccg gggtctggct cgcgccgcgt
     3601 ccgctgagtt tcatctccag ggtgctggag accgtcatca cggccgtccc agccacgcct
     3661 gcgaacagtc ccttgccgac attcgcggct atgtctccca tactcgtgct cattaggtgc
     3721 tcctttcgga tggtttgtat tgccttttct tcatcgaacg agcaagtcgg gggttacagg
     3781 aaggtcgcgg acgagtaggt cagtcgcgtg aatgactaca gccttcatgt atcacctcct
     3841 cgcaggatgc ggacccggtc gatcgaggcc cgaccttgtt tgccagcact cacgggcggc
     3901 aggcctgccc tttgtggcag gcgcctctgg ccaccggacc tttcggcgtg ctacacggcc
     3961 atccaggact agatgaggac cctggtgtgg ccgccaatgc gcacttgaaa agtccgtacc
     4021 cggaccagac cctgggataa ccacgcatcc gtccttccgg atgaaaatgg cccaaagtat
     4081 gcaggtggac cgggttgggc ctcttgcctt ccgtcagccg gcgatcagca cttcgagcga
     4141 gcgcgggccg tgtacgccct cgaccctgtc gagctcgata tccgaggtcg ccgacgggcc
     4201 tgagataaag gtgatagccc tgccttcgcc cttgaccctc cgttccaggc tggcgaaggc
     4261 ctcgggtacg aggcccacta tctgctgctc gcgcacgacg cacaggtggt agtcagggag
     4321 taaggtgagc gtccggcggc cctgcgccct gccggcatcg agcacgatgg tgcctgtctg
     4381 ggcaataccg agggcgcagc ccgtcagcac gccgtcgctc tcatcgagct cctggttcgt
     4441 aagccgggaa cgggcagcgt cgcgaaggat ctctatgccc tccgggatcc actctctcgg
     4501 gaggtaaggg gggatgacga ggcgtctcac gccgcgccgc ttcagggctt cttctatggc
     4561 cctggggagc acgtcgtggc ccacgcgacg gacgtctgcc tcgtactccg ccgtgcgctc
     4621 ggcgaagcgc gcgacgatct cctcgcgctc cgcgccgtct tccttgcggt attcgcgttc
     4681 gacggatacg tcttcgggac gctccgcctc tggtacgtcg cgggtggcgt gtcttatacg
     4741 ccacaggatc gtctctctcg cggtcgccac tagaccgacc tcctgttccg ccaccactct
     4801 cggaaggtct gctgtgggat aggcttgagg tcgcgtactg cggtccaacc ggcgagcctg
     4861 cctggaaggc gactaataga gcctccgcgg gcgaagggcc actggcctgc cctcgccaat
     4921 ctctgggctc cctcgtagag ctgtgggtcg gagaacaact ttgcgaccgt ctgcatcgcg
     4981 acgttctcag ggtcgagctt cgagcgcggg ccgcccatgt actgcttgtg acggacgacc
     5041 ctgcctcgga ggtggacgag gacctctggg atgttgatct tgaccggaca cacctgatag
     5101 caagccccgc aaagcgacga ggcgtagggc agcgagtccg atcctgggtc gccaacgccg
     5161 acgagctggg gagtcaggat cgcgcctatc gggcccgggt agacggagtt gtaggcgtgt
     5221 cctccggtcc gctcatagac ggggcagacg ttgaggcacg ccgagcagcg gatgcagttc
     5281 agggcctggc gccctatctc gtcagccagc acgtcggtac gcccgttgtc catgaggacc
     5341 aggtggaact ctcgcggccc gtcaccagcg tcgcccgaga cccctgtcca gaacgaggtg
     5401 taagggttca tccgctccgc tgtcgaggag cgggggagga gctgcatgaa gacctcgaag
     5461 tcccgccagg tagggatgac tttctctata cccatgaggg ttacgaggac cggcggcaac
     5521 gtcgtgcaca tcctgccgtt gccctcggac tcgacgacgc agatagtccc cgtctcggcc
     5581 acagcgaagt tcgcccccga gataccgatc ttagccttga ggaacttctc gcgcaggtac
     5641 aggcgggccg cctccgtcag gtcggaaggc ctgtcggaga ggtcctgtgc gccaagctcc
     5701 tccataaaga gctccctgat ctccgcccgg ttcttgtgga tcgctggcac caggatatga
     5761 gaagaggtct cccctgcgag ctggatgatg agctcggcga gatccgtctc gaatgccttt
     5821 ataccctccc tctccaagtg ctcgttgagc ttcgtctcgt cggtagcaat ggacttgact
     5881 ttgacgacct cttctgcgcc cccctctctt acgatcccgg agattatgcg attcgcttcc
     5941 tcggcgtcgc gcgcccagtg gacctcgcct ccggcgcgcg tcacggactc ttcgagctgc
     6001 agcaggtact cgtcgaggtg gcgcaggacg cgctccttga gggcgcgtcc tgcctccctg
     6061 agtccttccc agtcaggcat ctcttcgacg gcgagggcgc gcttgtggcg gatagtctgg
     6121 gttgccttcc cgagattgcg tcttagctgc gagtcggaga ggctctcctt cgccgactcc
     6181 tcgaacgtcg gagtcgggcc aggggagaag gcgtctgact tcttcgtctc cactatccct
     6241 cctcggcttt gctggcgagg atctgggcga gatgcacggt ctccacccct gtacgaaggc
     6301 gggctagtcc gccgccgatg tgcatcaggc acgagttgtc ccctgctgtg cagacttcgg
     6361 catcggtatc gaggacgcgc cggatcttat ccgagagcat cgcggcggag gtgtccgcgt
     6421 tcttgaccgc gaaggtcccg ccgaacccgc agcactcctc ggccccggag agctctacga
     6481 ggtcgatgcc ccgcacattg cgcaacagct ccagcggagc atctccgacc ctgagcatcc
     6541 tcagtgagtg gcaggtcggg tggtaggtaa cccggtgcgg gtagtaggcg ccgacgtcgg
     6601 agacgccgag ctttctgacc aggaactcgg agagctcgaa gactttcggg ccgagttcct
     6661 cgacctgttc ggtgagacgc ccgtccccgg cgaggtcagc ggccatcggg tagagctcgc
     6721 ggaccatgct cacgcaggag ccggagggcg cgacgacggc ctcgtaaccg ccgaagatct
     6781 cgacgaagcg cctcaccagg gggatggcct cccgttggta gccggtgttg aagtgcatct
     6841 ggccgcagca cgtctgcgcg tagggaaagt ctacctccac accgagacgt tcgagaacct
     6901 ccacggtagc ctgtcccgtc tctggaaaga gcgtgtcgtt gaagcaggtt ataaagagcg
     6961 ccacgctcac cgctatagtc cccttctcct ctgcgtctaa gagcaccatg atagaccatt
     7021 ccagggccct gcggccctcg ttgccctcgc ggaggctgcg ctcgcgccgg accaagagcg
     7081 gggttacggt gggactcatg tggagataat ggagtcatgg ccacgtcctc ggcggtcgag
     7141 tacgcgcgtt cccggcacgc cagtacggcg tccataaaac cagagtcgcg ttcctggaag
     7201 ccggcatctc cgaagaggac gtagaccgct gggtccagac ggcctccatt ctgcactcca
     7261 acggtgacgc cttcgacatt gctgtgaagg gcggccgcat cgtcggggtg cgcggcagga
     7321 gcgtggatcg agtcaacaag ggacgccttg accccaaaga cctcttcggc tggcaggcca
     7381 acaactcaga agaccgcttg acaagaccgc tcgtgcgtaa agacggagag ctcgtcgagg
     7441 cgagctggga cgaggccttc gacctcatcg tcagcaggtc gaaggctctg ctcgaggaga
     7501 agaggccgct ggcgttcggg ttctacacga cgggccagct ctttctcgag gagtactata
     7561 ctctaggagt tatcggtaag gctgggcttg gtacgccgca catggacggc aacacccgcc
     7621 tttgcacggc gaccgcggcc caggcgctca aggagacctt cggcaccgac ggacagccag
     7681 gttcctacgc cgacatagat cacgccgaca ccatactcca cttcgggcac aacctcgccg
     7741 agacgcaggc ggtgacctgg atgcgcgtcc tcgacaggct cgccggaccg aactcgccga
     7801 agctcgtcgt cgtagatccg cgtcccacac ctgttgcgaa aagggtgacc gtccacctcc
     7861 ccataaagag cggtaccaac gtcgcgcttc tcaacgcgtt cttgcacgag atcctggaga
     7921 acggactcta cgaccaggag tacgtcgagg cccataccaa agcacacccc atccgtgaag
     7981 aaatcatcca tttggggtag aaagaaatca cccattttgg gtatgtgctc aggcccgcct
     8041 cggtactaca atctgactaa tcgtcaagcc gggcaccgtc tgaatctacc ccctttcctc
     8101 caaagagcag cccggctttt ctcttgcctc tgccggaggt cctgggccgt ccaaaccgga
     8161 gcctggtgaa gatactcatt tctgactatg ctttgatgtt ttcgagttaa cgctcaacca
     8221 acaccccgcc tctacgatgg cccatacaac atgcaaggga gagaaaggtg agtctgagag
     8281 cggcagccgc atgggcggag gagctgctgg tggctggttt actggattca ttgtggattc
     8341 atccacctct gctccaatgt tggcgcgcga agagcaaccc acgggcggat ggaagagaag
     8401 ctgatgccgg atttcgatac gcgaaagcct cagggatcta acgaaccaaa caggcctcgt
     8461 attgcgttga tcgcaaacag gctgagcagg ctgttgagcg cgagcaaagt tcgcatcctg
     8521 tcgattgcga gcacgcttcg ccgtttcttg atggcgaaca ggcttcgtac tttgttgatt
     8581 ggtggtggca tgatgttgtt cgtggcggct gccgcacttg ggctgctcat ctactcgtcc
     8641 atcgagttgg ccgatggtgg ccagaaagtc accgatttgc agcagaaaat cgaaggcgcg
     8701 acgctcacgc cgttggaagg gtgtcccgtt ggcggtgaca taaatcaaag caagatcagg
     8761 gatatggtac atggattttc cagtaccaag aagatagtct tcgtacgcgg ctccgacatc
     8821 acttaccttt actatttggg tgtcgggggc acaatcgata ttgtcgccaa catcgaactg
     8881 tacatgatca gcaccgacgg cacctccctt actcggctca ccaagactac ggcgaaggaa
     8941 tacggtgcca accgcttgcc cgacggcagg aggatgaata tcctctggga taccgatggc
     9001 agaacgatac gtggcataag ctacttcgat agcgaaccag gcaccactct cagcggtcgc
     9061 ccccccatac ccacctgacg ctattgacat gtatgaagtc aacgtcgatg gtaccggcct
     9121 gacccacctc accaacaccg aaactcagga gggcatactt acctgggtct ggaagtaagg
     9181 acggcgggac gctaagcgta acggccaggg cacctaatca accctggcgc ttccctcgca
     9241 aattgccgga gtgccttcga cgaaaatgtc acgactgcgc tcagaaatgg tgtagacggc
     9301 gttgtcggtg tagaccacta agcagaataa acaacaccgt ctacaccagc aacaccgttt
     9361 cgtgagggac atatcctctt gacggcagtg cacttccgcg gcggggtatc cttcggggtg
     9421 tcccggccct tcactttgct acgacgggtg ggcgacagac tagcatccca cccgtcgcgg
     9481 catcaaagca cacatctgcc cgcatcgaaa cgtgttttaa aggcactact acggatatta
     9541 acaacgctta atggccagga tgttagatta ggtagtccat agtccggccg gggcttcccc
     9601 ccctccgtcc cgaccggacc ttcgcatcga ccaggccggg gctcgaaagc cccggccttt
     9661 atactgcttt tggccgcgct ctacccccat cctcttgcag cgcggccatc attttagggt
     9721 ttatccatgg ggttgttttc cggtgaacgc ttcgccccgg cccgatgccc taagcttagg
     9781 gcactaggtc ccctcccctc cgactgccct tactgcctca agacgaacga gcatagcaag
     9841 ggtgagtccc gcgtacagtg cccacacccc caccagcgta agcagcccgt ttcttaaggg
     9901 tttgggctcc ggcacggccg ggggcacttg ggcctcctcc cagacgatgg ccgtgaggtt
     9961 cttgccgggc gcctcggaga ggcgttcgga cgcaacgctg gctaaggcgt tgaccgtctg
    10021 ggcggccttc tttcggtcgg tgcctcggta tgataggtct atgaaagttg tgccccgcac
    10081 ccgacttact tccaagttgt ctagtagctc agcggaaccc gcttgcagcc cgaggcttcg
    10141 cctggcctct ttggccacgg ggcgactggc tgctaggcgg accatcgccg gggtagtttc
    10201 ggtgagcctc gtgtccggcc ctggagcggg tggcccaatt ctt
//
LOCUS       DATMJA010000351         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_106806, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000351 DATMJA010000000
VERSION     DATMJA010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106806"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1255)
                     /gene="coaA"
                     /locus_tag="VNT12_08475"
     CDS             complement(347..1255)
                     /gene="coaA"
                     /locus_tag="VNT12_08475"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HWI46193.1"
                     /translation="MRLSREDWARLPADASFALSDDDLRKIGAHLSREEATQVYLPLS
                     RLLHLHVGATQNLYRAARAFLAREEKEVPYVVGIAGSVAAGKSTVARVLRALISRWPG
                     SPEVGLVSTDGFLYPNRVLRSRGLMERKGFPESYDLPLLLRFLADIKSGRDGVSAPIY
                     SHLTYDILPDKTQVLGRPDVLVVEGLNMLEAGLSEPSEGHRVFVSDYCDFSIYVDAQE
                     RHIKEWYLERFLRLREEALGDNTAYFHRFAELSVEQAKAEALRVWDEIDHPNLKENIE
                     PTKDRARLILAKGHDHSVQSVRLRKI"
     gene            complement(1554..>2582)
                     /locus_tag="VNT12_08480"
     CDS             complement(1554..>2582)
                     /locus_tag="VNT12_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWI46194.1"
                     /translation="IAEVLLDSELRGHESHGVHLLGFLADAYRGGGMNPQPRIRVLRE
                     TQGALLLDGDRSHVVGAVRAMRWCIERARERKGMAVAGVRNSQLIVPGFYARMAAEAG
                     LICFACANAVPMVAPPGGRTPTFGTNPFAYAIPAGRYPPVVLDVATTTGAAFKVRLAS
                     QRNRPVPEGMILDGEGRPTMDPNEFIHGGLMAPLGSPAAPHKGFGLALVFDALAGVLT
                     GAAFARDFPSEPVTAGSAFFWALDVEAFLPREEFLGRMEAQIDQVKAGERLAGVDELF
                     LPGERSHRRYSELTTRGTVPLSGSTWEALTKACASLSIAPPPALAAEPSQLDSEEASR
                     ERGTHYKP"
BASE COUNT          470 a          803 c          837 g          472 t
ORIGIN      
        1 ctgagatctg gacgatccaa cccggcgggg gtggtaggca gcaagtcacc gacaacgcta
       61 atagggacat cgatccttac tggggcagtc aatagcgatt gcttcctccg ggtagagccg
      121 gagaagtgag tttcgcagag agtaaggaaa tagggagccc ggaggcgcca tcgcgcgtat
      181 tccgggctct tctttgttgg aggttgctcc gtctcgtgcg gcctctcctc agcaccggag
      241 tggcgagccc agtgggtaga aaggatgtag tggtatcgaa acactctctg tgaagagatc
      301 acccaattgc gctagagaga aatctcgagc cttccgcaat tcgctgtcag atcttcctca
      361 ggcgcacgct ctggacggag tgatcgtgtc ccttcgcgag gatcagacgc gccctgtctt
      421 tcgtcggctc gatgttctct ttgaggttgg gatggtcgat ctcgtcccag acgcgcaggg
      481 cctcggcttt ggcctgctcc accgagagct ccgcgaaacg gtggaagtaa gctgtgttat
      541 ccccgagcgc ctcttctcgc aggcgcaaga aacgttccag ataccattct ttgatgtgcc
      601 tctcctgcgc gtccacgtag atggagaagt cgcagtagtc ggatacgaag accctgtgtc
      661 cttccgaggg ctccgatagt ccggcctcca gcatgttgag gccttccacg acaaggacat
      721 caggacgccc taagacctgg gtcttgtcgg ggaggatgtc gtaggtcagg tgcgagtaaa
      781 tcggggcgct caccccatcg cgaccggatt tgatatctgc gaggaaccgc agaaggagcg
      841 gcaggtcgta gctctcgggg aaacctttgc gttccatcaa cccccgagat cgcagcacgc
      901 ggttggggta gagaaaaccg tcggtgctca ccaacccgac ctcggggctc cccggccagc
      961 gggagatcag ggcgcgcagt acccgggcca ccgtactctt tccggcggcg acgctgcctg
     1021 cgatgccgac gacgtagggc acctccttct cctccctggc cagaaacgcg cgtgctgccc
     1081 ggtacagatt ctgcgtggcg cccacgtgga ggtgcaggag ccgcgagagg gggagataga
     1141 cctgtgtcgc ttcctccctg gagaggtgcg cccctatctt ccgcagatcg tcatcagaca
     1201 aagcgaacga ggcgtctgcc ggcaacctgg cccagtcctc gcgggagaga cgcacaaaag
     1261 ggtggagact gctctgcctg gtgtccgtgg tagcgtcctc ctctcatcct ctctacggag
     1321 tcccgacgac agtcttacac aaactctggc atcaggtatc gtgatgatgt gcgaccggta
     1381 atccccctcg ccgtaagcac tgtggacttc cacgctgatc ggctcgtaca cggttctcct
     1441 cggcccgagg gtccggccgg aacggagggg ggaaagtccc ggccgaatcc cgcggttacc
     1501 gagaccctat caccctgagt gtcagatgcc gttaatcgta ggcgaacgtt acgttaaggt
     1561 ttgtagtgtg tacctcgttc tctgctagcc tcctcgctat ctaactgact gggctcggcc
     1621 gcaagggcgg gaggcggtgc gatagacagg gaggcgcagg ccttcgtgag cgcttcccag
     1681 gtcgaccccg agagcgggac cgtgccacga gtggttagct cgctgtagcg gcggtggcta
     1741 cgttcgcccg gcaagaacag ttcatcgacg cctgccagcc gctcgccggc ctttacctga
     1801 tcgatctggg cctccatccg ccccaggaac tcctcgcgcg gtaagaatgc ctccacgtcg
     1861 agcgcccaga agaaggcgct gccggccgtg acgggttcgc tgggaaagtc gcgggcgaag
     1921 gcggccccgg tcaagacgcc ggccagcgcg tcgaagacca gtgctaggcc gaagcccttg
     1981 tggggcgctg ccggggaacc gagcggtgcc atgagcccac catggatgaa ctcgttcgga
     2041 tccatggtcg ggcggccttc gccgtccagg atcatgcctt cgggcaccgg acggttgcgt
     2101 tgcgacgcga gccggacctt gaacgcggca ccggtggtcg tggccacgtc gagtaccacc
     2161 ggtgggtagc gtccggccgg gatagcgtag gcgaacgggt tggtgccaaa ggtaggtgtg
     2221 cggccgccgg gcggtgcaac catcgggacg gcgttggcgc aagcgaagca gatcaggccg
     2281 gcctcggccg ccatccgggc gtagaaccca ggcacgatta gctgagagtt gcgcacgccg
     2341 gcgaccgcca tgcccttgcg ctcgcgggct cgctcgatgc accaacgcat cgcccgcacc
     2401 gccccaacca cgtggctgcg gtccccgtcg aggagcagcg ctccctgcgt ctcccgcagc
     2461 acccggatgc ggggctgcgg gttcatgccc ccaccgcggt aggcatcggc caggaagccg
     2521 agtaggtgaa cgccgtggct ctcatggcca cgcagttccg agtccagcaa gacctcagcg
     2581 at
//
LOCUS       DATMJA010000352         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_106841, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000352 DATMJA010000000
VERSION     DATMJA010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106841"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VNT12_08485"
     CDS             <1..1043
                     /locus_tag="VNT12_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46195.1"
                     /translation="EIVVRDGPEVAIRDSTVQAKSVSKELRSYPRDMLSSPPDVREAS
                     FTLAPGDGSVHNDAAGRTAERVGTSFGGVTGRIDELVSVERLSAAVVALSILAAFFWG
                     AAHALTPGHGKAVVAAYLIGARGTARHAGILGLTVTLTHTAGVFVLGAVTLYLSRYIL
                     PEVLYPWLSVASGLLVVLIGLALLYRHLDKMSHDKKAGRTHVEHTHSHDGEHSPGGSH
                     AHDHHTHSHGGRTHSHLPADDSKITWRGLMALGVSGGLVPCPAALVLLLGAISLDRLG
                     FGMVLVLAFSAGLAVVLTGIGLLMIYARKLFERFTFEARGPRLLPVASASIITLAGVG
                     IALGALHQTGIV"
     gene            1271..1834
                     /locus_tag="VNT12_08490"
     CDS             1271..1834
                     /locus_tag="VNT12_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWI46196.1"
                     /translation="MKTGTGVGIACAIGVAGGIMIGSRAVKIYDWRTEWTIPAPMPFV
                     YKAMTSRQAVREWWPDMELVEDNGDDDLQVGSTVSFRVHQSPEVARLAPPFRIHCLYT
                     DVEPEKRLRETVNGDLSGVLETLFHEQQDGTQITFSWYVRVTNPALNLLGYAAERMYR
                     HSHDSVMRSGEKGLSEYCRRRTSAQVV"
     gene            complement(1921..2079)
                     /locus_tag="VNT12_08495"
     CDS             complement(1921..2079)
                     /locus_tag="VNT12_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46197.1"
                     /translation="MGRRRDYDEGYEDARRYMMDQETGSRRGSDGAFTAAVFIKYAAI
                     VIVVLIIA"
     gene            2215..>2600
                     /locus_tag="VNT12_08500"
     CDS             2215..>2600
                     /locus_tag="VNT12_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI46198.1"
                     /translation="MPNVTIIGAGLAGSEAAWQAAELGCSVELWEMRPERETPAHRTE
                     HFAELVCSNSLGNRSLETASGLLKEELRRLGSVILHCADANPIPAGGALGVAREDFPR
                     AVTETIHAHPNIKVIRKEVTYIPQGPT"
BASE COUNT          477 a          804 c          829 g          490 t
ORIGIN      
        1 gtgagatcgt ggtcagggac ggacccgaag tggctatcag ggactctacg gtacaggcga
       61 aaagtgtgtc gaaggaactg cgctcctatc cgcgagacat gctctccagc ccgcccgatg
      121 tccgcgaggc gtccttcacg ctcgctcctg gcgacgggtc ggtccacaac gacgcggcgg
      181 gccggacggc ggagcgcgtc ggaacgagct tcggcggcgt gacagggcgg atcgatgagc
      241 tggtctccgt agagcgtcta tcggcggcgg tcgtagcgct gtcgattttg gccgcattct
      301 tctggggagc ggcgcacgcc ctgaccccag gtcacggcaa ggcagtcgtg gcggcgtacc
      361 tgatcggggc ccgtggaacc gccaggcacg cgggcatcct cggactgacg gtgacgctaa
      421 cccacacagc aggagtgttc gtcctcggag ccgtgaccct ctacctgtca cgctacatcc
      481 tgcccgaagt cctctatccc tggctgagcg tagcctccgg actgctcgtc gtcctgatcg
      541 ggctcgccct cctttaccgc catctggaca aaatgtccca cgacaagaaa gccgggcgca
      601 cgcacgtgga gcacacacac tcgcatgatg gcgaacactc ccccggtggt tcacacgccc
      661 acgaccatca tacccattct cacggcgggc gtacgcacag tcacctgccg gcggatgact
      721 ccaagataac gtggcgtggg ttgatggcgc ttggcgtcag tggtgggctc gtgccgtgtc
      781 cggctgcgct cgttctgctt cttggtgcga tctcgctcga caggctgggg ttcgggatgg
      841 tgctcgtcct cgcgttcagc gcggggttag cggttgtgct gaccgggatc ggactgctga
      901 tgatctacgc tagaaagctc ttcgagcggt ttactttcga ggcgaggggg ccgcgcttgt
      961 tgccggtcgc gagcgcctcg atcatcacat tggccggggt gggtatagcg ctcggagctc
     1021 tccaccaaac cgggatcgtt tagtcgagaa tcccggcttg ttacactggt tctgaggtac
     1081 cgggtcctgg cgtagtcggc ccaggtgctg gggcgattac gggacgtctc gagaaggaga
     1141 gtagcatgaa gccggaacgc gtgggcgaac cgggcgtgga tgaaagattg gctcgacttc
     1201 cgtcgctgga tgctctgcgg gagcgccgct cgacctccag atccggaggg ccgaagagga
     1261 ggctcgtccg atgaagaccg gaacaggtgt cggcatcgcc tgtgctatcg gcgtggcggg
     1321 gggtatcatg atcgggtctc gcgccgtcaa gatctacgac tggcgcacgg aatggactat
     1381 tcccgcgccg atgcccttcg tctacaaggc catgacctcg cgccaggcgg tacgtgagtg
     1441 gtggcctgac atggaactcg tcgaggacaa cggagatgac gacctccagg ttggcagcac
     1501 cgtctctttc cgggtgcacc agtcccccga ggtggcacgc ctcgctccgc ccttccgcat
     1561 ccactgccta tacacggacg tggagccaga gaagaggctg agggagacgg tgaacggaga
     1621 cctgagcggt gtgctagaga ccctgttcca tgaacagcaa gacggcaccc aaataacatt
     1681 cagctggtac gtgcgcgtca ccaaccccgc tctcaacctc ctggggtacg ccgccgagcg
     1741 catgtaccgt cacagccacg acagcgtaat gaggtcaggt gagaaagggc tctcggagta
     1801 ctgccggcgc cgaacgagtg cacaggtcgt ctgacgcccg atgacacaac taccctgaca
     1861 agctgaatcg ctgaccagct aaactgctcc ccgtatggca ccgacgattc ggagcaccac
     1921 ttaggcgata atgagcacga ctatgactat agcggcgtac ttgatgaaga cggctgccgt
     1981 aaaggcgccg tcgcttccgc gccgcgagcc ggtctcctgg tccatcatgt acctgcgggc
     2041 gtcttcgtag ccctcatcgt agtctcttct tctgcccatg tatctccctc tggaaggtgt
     2101 tctggtagcc tctattacag ctgctgcccg acccagaagg acctttccgg agcacggcct
     2161 tgacttggcg tagaataacc tccctgcaaa gccacgagcg gaggatcttc ttcgatgccg
     2221 aacgtaacca ttatcggtgc cgggctcgcc gggagcgagg cggcctggca ggccgcagaa
     2281 ctcggctgct cggtcgagtt gtgggagatg cggcctgaaa gagagacccc tgcccaccgc
     2341 acggagcact tcgccgagct cgtgtgctcg aactcgctcg gtaatcgttc cctggagacg
     2401 gcctctgggt tgctcaagga ggagctcagg cgtcttgggt cggtgatcct acactgcgcc
     2461 gacgccaacc cgattccggc gggcggcgcc ctcggggtgg ctcgcgagga ctttccacgc
     2521 gccgtcaccg agacgatcca cgcccacccg aacataaagg tgattcgtaa agaggtcacg
     2581 tacatccctc agggtccgac
//
LOCUS       DATMJA010000353         3197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_166405, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000353 DATMJA010000000
VERSION     DATMJA010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166405"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VNT12_08505"
     CDS             <1..516
                     /locus_tag="VNT12_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HWI46199.1"
                     /translation="VTLLGKAVEEAMNEQIKNELYSAYQYLSMAAYCESENLPGFAQW
                     MRAQSREETEHAMKFYDFILDRNGRVVLREIDGPVVEFGSPLEVFEQALEHEKEVTAM
                     IHDLYGLAVRENDYASQMFLQWFVTEQVEEEKNAGDVVETLKMVGDKSEALFLLDREL
                     GQRRTEGQPSG"
     gene            583..729
                     /locus_tag="VNT12_08510"
     CDS             583..729
                     /locus_tag="VNT12_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWI46200.1"
                     /translation="MTNEEYRSFVLARARTAVLGTVRADGRPHAAPIWFDLDGDTLVL
                     DRRV"
     gene            991..1686
                     /locus_tag="VNT12_08515"
     CDS             991..1686
                     /locus_tag="VNT12_08515"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWI46201.1"
                     /translation="MNERREDSGGNHGLNDLANRLREAVSRPLAEWREVYESFSPVDL
                     ESGERRPRVPPPGADTRRAAVLVPIIVESEDVRVVYTVRKDDLADHAGQISFPGGSIE
                     ARDTSLLETALREAEEEIDLDPELVEVVGELEEMYIPPSNFRVSPFVGILTPEAEMVL
                     APDEVEEIFTVPLKILTAPETFRKVLWRREGRDYKVPVFAIEGPVPRNIWGATAAMTA
                     ALLARLGWREYRR"
     gene            1696..2088
                     /locus_tag="VNT12_08520"
     CDS             1696..2088
                     /locus_tag="VNT12_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46202.1"
                     /translation="MLRVVAMVLFGLMFLAEAGDLYGLILTLADPVPAADRFGITASA
                     EVLRSTLLLSIALVVCFGALLALVGLLARMPALFHTSALVCAIGYLVYGLFQVADGAL
                     QLGSAIVVVAGLIYVVLGGLAYAMYRSV"
     gene            2211..>3197
                     /locus_tag="VNT12_08525"
     CDS             2211..>3197
                     /locus_tag="VNT12_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI46203.1"
                     /translation="MATETERDEGVRRVYKDHNLHVLWGVTLMAVLGTSSVTPAFPTI
                     VREFGISSGQVGLLITFFTLPGIVMTPLLGVLSDRYGRKKILVPALLLFGFAGGACAF
                     ARNFDVLLALRFLQGMGAAALGTLNVTVIGDIYDGRERSNALRYNCSVLSVGTASYPA
                     IGGLLATFGWFYPFAFPFAAISIAIVVMFSLHNPEPRNDDRLRDYFGSVWENLHDREV
                     FGLICASLLTFIVLFGPQISYLPILMNARFDAPSYVIGAVLSGASLTTALTSTQLGRL
                     TGRFSEKSLLKVAFVLYAIALIVVAYTPSLPFLALPAVLFGVAQGINLPNVFS"
BASE COUNT          559 a          972 c          995 g          671 t
ORIGIN      
        1 gtcaccttgc ttggcaaagc ggtcgaggaa gcgatgaacg agcagatcaa gaacgagctc
       61 tattcggcct accagtatct ctcgatggcc gcgtattgcg agtcggagaa ccttcccggc
      121 ttcgcgcagt ggatgcgcgc tcagtccagg gaggagacgg agcacgcgat gaagttctat
      181 gatttcatcc tcgaccgcaa cggacgcgtc gtcttgcggg agatagacgg gccggtggtc
      241 gagttcggct cgccgctgga ggtcttcgag caggcgctgg agcacgagaa agaggtcacc
      301 gcgatgatcc atgatctcta cgggctcgcg gtaagggaga acgactacgc gagtcagatg
      361 tttctgcaat ggttcgttac ggagcaggtc gaggaggaga agaacgcggg ggacgtcgtc
      421 gagaccctga agatggtcgg tgacaagagc gaagcgctct tcctgctcga cagggagcta
      481 ggccagcgcc ggacagaggg gcagccatcc ggctgacgaa ccgattttct ctatggctac
      541 ctgcttctag cgtgtcggtg ggggattaca gtggcgcaac agatgacgaa cgaagagtac
      601 aggagcttcg tgctcgcccg ggccaggacg gcggtcctcg gtacggtgcg tgctgacggc
      661 aggccccatg cggccccgat ctggttcgac ctggacggcg atacgctggt attagatcgt
      721 cgcgtataag aacatttctg attgacatcg ggttttggaa gggctgccct tccaaaaccc
      781 acgctggcca gcatttcagc aagtacctgc cagcgaggct ccctggcaga cgatgcgggc
      841 ctcatacacc gcagagtggg caccacaaac gcaagccgct cccaaaaatg ctgactagct
      901 gaagtgctga caagcgaagg ccgcaggccg agcgtgctga ctggctgacc agttgaaatg
      961 ctggtttgtt ggtacgctat gatctcggta atgaacgaac gtcgggagga cagcggcggg
     1021 aaccacggct tgaatgatct cgcgaatcgg ctgcgtgagg cggtctcccg cccgttagcc
     1081 gagtggcgcg aggtctacga gagcttctcg cctgtggatc tggagtcagg tgagagacgt
     1141 ccgcgggtgc cgccgcctgg tgctgacact cgccgtgcgg ccgttctggt tccgatcatc
     1201 gtagaaagcg aggatgtgcg ggtcgtctat acggtgagga aggatgatct cgccgaccac
     1261 gccggccaga tctcctttcc cggcggcagc atagaggccc gtgacacttc gctcctggag
     1321 accgccttac gcgaggccga ggaggagatc gacctcgacc ccgaactggt cgaagtggta
     1381 ggagagctgg aggagatgta catcccgccg tcgaacttcc gcgtcagccc gtttgtcggg
     1441 atcctgaccc ctgaggccga gatggtactg gctcctgacg aggtagagga gatcttcacg
     1501 gtccctctga agattctgac cgcccctgag acgttccgca aggtgctctg gcgccgcgaa
     1561 ggccgcgact acaaggtgcc tgtgttcgcc atcgagggtc ctgtcccgcg aaacatctgg
     1621 ggtgcgacgg cggcgatgac cgcagccctg ttggcacgcc tgggctggcg ggagtacagg
     1681 cgctaggggg acggtgtgct acgagtggtc gccatggtgc tcttcggcct catgttcctg
     1741 gccgaagccg gggacctcta cgggctcatc ctcacgctcg ctgatcccgt gcctgcggcc
     1801 gaccggttcg gcatcaccgc cagtgccgag gtcctgcgca gcaccttact cttgagcatt
     1861 gcccttgtcg tttgctttgg cgcgctcctt gccctggtcg gattactcgc ccggatgccg
     1921 gcgctctttc acacgagcgc cctggtgtgc gcgatcggat acctcgtgta cggcctcttt
     1981 caggtggctg acggcgcgct gcaactcggc tcggccatag tggtcgttgc cggtttgatc
     2041 tacgtggtgc tcggcggtct cgcttatgcg atgtaccggt ctgtgtaata gagcgcttca
     2101 gcatttcaac ttgtcgcatg cggggccctt gttgacaggc ggaggccgaa gcgtgctgag
     2161 agtgtgtctg atcccacctc cgaacgcacc acctgataga ttctcctacc gtggctacgg
     2221 agaccgagag agacgagggc gttcgccggg tctataagga ccacaacctc cacgttctgt
     2281 ggggcgtaac tttgatggcg gtcctcggaa cctcgagcgt aacgcctgcc tttcctacca
     2341 tcgtacggga gttcggcatc tccagcggac aggttgggct cctgatcacg ttcttcacac
     2401 tccccggcat cgtgatgaca ccgctcctcg gcgttctctc agaccgttac ggaaggaaga
     2461 agatcctggt ccccgccctc ctcctattcg gctttgccgg tggcgcctgc gccttcgccc
     2521 gcaacttcga tgtgctcctt gccctccgct tcttacaggg tatgggggca gccgccctcg
     2581 gaacgctcaa cgtcacggtg atcggcgaca tatacgacgg ccgcgagcgc tcgaacgccc
     2641 tcagatacaa ctgcagcgtc ctctccgtgg gaacggcgag ctaccctgcg atcggcggtc
     2701 tgctggcgac cttcgggtgg ttctaccctt tcgcctttcc cttcgctgcg atatccatag
     2761 ccatcgtggt catgttctcg ttgcacaatc cggagccccg caacgatgat cggctgaggg
     2821 actacttcgg gagcgtgtgg gagaacctcc acgacaggga ggtcttcgga ctgatctgtg
     2881 cgtccctgct gacgtttatc gtgctgttcg ggccgcaaat atcgtacctc ccgatcctca
     2941 tgaacgctcg cttcgacgcc ccttcctacg ttatcggggc tgtcctctcc ggtgcctccc
     3001 tgacgaccgc gctgacctcg actcagctcg gccgcctgac cggccgcttc tcggagaaga
     3061 gcctcctgaa agtggctttc gtactctatg cgatcgccct catcgtagtc gcttatactc
     3121 ccagcctgcc ctttctcgcg ctccccgccg tcctttttgg cgtggcgcag gggatcaacc
     3181 tccccaacgt cttctcg
//
LOCUS       DATMJA010000354         3877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_640101, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000354 DATMJA010000000
VERSION     DATMJA010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640101"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VNT12_08530"
     CDS             <1..973
                     /locus_tag="VNT12_08530"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWI46204.1"
                     /translation="LFQSVMEIKTPPCASTSDVEKHLRELRSRVGSWAAACGASLASA
                     GTHPFSRYRDQKVTDQERYKNVLRTLRWIAQREVIFGQHVHVAVPGPEEAIIAHNRLA
                     EQTPILLALSANSPFWQGFDTGFESARVKIFESFPRAGLPPAFPDYEAFEGYVDLMVE
                     SGAIEDYTFCWWDVRPHPKIGTIELRVLDSQTNLKNTLALTALTQCLVAQVLDEVGPH
                     EPYNMYLAVENKFRAARYGIDAAFYDVHDKITVPARDLARNLVERLKPHAQDLGCESE
                     LEGVLEIVEGGTGSQRQREIYKESGNFLDVVAFLIEGTRPALAEEQS"
     gene            complement(1019..1300)
                     /locus_tag="VNT12_08535"
     CDS             complement(1019..1300)
                     /locus_tag="VNT12_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWI46205.1"
                     /translation="MVTAIVLVRTERERVQEAAQAMLAIDGVTEAYSVTGPYDLVVMV
                     RIPDFESLADVVPERIAQVPGVARTETMVAFRAYSNYDLDRAWSLGFEK"
     gene            complement(1436..2527)
                     /locus_tag="VNT12_08540"
     CDS             complement(1436..2527)
                     /locus_tag="VNT12_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46206.1"
                     /translation="MGVRIERRSHRAAPDLRRQRPSGRPRRPRSGPKERRRRTLPAWL
                     TFVGSILLLVVVFGFIFFVARGCVATQQSTQVRKYVTSADSFLSDSANAGRGELQRIL
                     GNAGGNPARLDEEALAQAANRTEQQYVQALRQEEIPPEFEDAHHYVVTALGIRAQATD
                     KLVQAATGDASGFAGELATAVEDYRTSDSIVANYYIPAVKHSLETVGQTSDQTYLEEP
                     QSFMDYGALGFDTAPVSGSARSDPNALHGVRVTAVKVAGKQMYSGGNVVLTGADEPTF
                     AVTVENDGDVPETGVEVEVILNTSAERQSESKTIARLEPKKMTMVEVGGFIPGELHET
                     ARVTVEARPVEYEKRTANNTLTGRVTFGI"
     gene            complement(2539..2622)
                     /locus_tag="VNT12_08545"
     tRNA            complement(2539..2622)
                     /locus_tag="VNT12_08545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2586..2588),aa:Leu,seq:cag)
     gene            2759..>3877
                     /locus_tag="VNT12_08550"
     CDS             2759..>3877
                     /locus_tag="VNT12_08550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWI46207.1"
                     /translation="MTETRYIEGQGRYALAEMVGRGGFATVWRARSLKGHLRGKDVAV
                     KVIPVYSAGERSRALREGQIAEGLRHKNIVETLEVIPGDHEIYLVTEYVRGMPLDEAA
                     KGYDVEEIVDSLAQILEALVYAHGQGIIHRDIKPQNALVDARGRVKLTDFGVAFRAGD
                     TRLTRVGFAVGTPGYIAPEILDGADPTALTDIYAVGATARTLLASQPYEPPPRLREFV
                     DRTTSPNPDHRPQSAWAAVKLLTGRKAPAGSISPGGRKVPDRLSGGFKDGALRIINGV
                     AAGCLGYLLAAQILADGAQALGIAAGLGVLAYLLPRLGALGVIVALAVLLIRNGAGLG
                     FSALLPVLGGVWVLGAGSANGDVRRLPLGPALAMPLALL"
BASE COUNT          675 a         1268 c         1211 g          723 t
ORIGIN      
        1 gctgttccag tcggtgatgg agataaagac tccaccctgc gcttcgacct cagatgtcga
       61 gaagcacctg cgcgagctac gcagcagagt cggctcgtgg gccgcagcct gcggcgcctc
      121 gcttgcctcc gcggggaccc atcccttctc ccgctacagg gaccagaagg tcacggacca
      181 ggagcgctac aagaacgtcc tgaggacgct gcgctggatc gcccagcgcg aggtgatctt
      241 cggccagcac gtccacgtgg ccgtcccagg cccggaggag gcgatcatcg cccataaccg
      301 tctggccgag cagacgccga tcctcctggc tctctcggca aactctcctt tctggcaagg
      361 attcgacaca ggcttcgagt cggcaagggt gaagatcttc gagagcttcc cccgtgccgg
      421 cctccctccc gccttccccg actacgaggc attcgagggt tacgtggacc tgatggtcga
      481 gtccggcgcg atagaggact acacattctg ttggtgggac gtgcgcccgc accccaagat
      541 cgggaccata gagttgaggg tcttagactc ccagaccaac ctgaagaata ccctggcgct
      601 taccgccctg acccagtgcc tcgtcgccca ggtactcgat gaagtcggcc cgcacgaacc
      661 ctacaatatg tatctggcgg tagagaacaa gtttcgcgcc gccaggtatg gtatagatgc
      721 tgcgttctac gacgtccacg acaagatcac ggtcccggcc agggacctcg cccgtaacct
      781 cgtcgagcga ctgaagcccc acgcccagga cctcggctgc gagagcgagc tagaaggcgt
      841 cctggagata gtggaaggcg gcacgggcag ccaacgccag cgcgagatct acaaggagag
      901 cggtaacttc ctcgacgtcg tcgccttcct catagagggt acccgccccg ccctcgccga
      961 ggaacagagt taggtgtcag gtttcgggag gatttgctga tgcctgacac ccgtagcctt
     1021 acttttcaaa ccctaagctc caggcgcggt ccagatcgta gttggagtaa gcgcggaagg
     1081 cgaccatggt ctcggtgcgg gcgacgcctg ggacctgggc tattctctcg gggactacgt
     1141 cggccaagct ctcgaagtcg gggatacgga ccatgacgac gagatcgtag gggcctgtga
     1201 cggagtaagc ctcggtgacg ccatctattg cgagcatggc ctgggcagct tcctgcacgc
     1261 gctcccgctc cgtcctgacg agcactattg ccgttaccat gtagactcct taaagcccgt
     1321 ttcgaaccat cgaagtagta cacatcgctt tccggccctc agagatctgt tcccagagaa
     1381 atcctgaagc ctgttgccgt gctgtacgga ggtccaatct atcggcacag cgcacttaga
     1441 tcccaaacgt taccctcccc gtgagcgtat tgttggccgt acgtttctcg tactccacag
     1501 gcctggcctc gacggtgacc ctggcggtct cgtggagctc gccgggtata aagccgccga
     1561 cctcgaccat cgtcatcttc ttgggttcga ggcgcgctat ggtcttgctc tcggactggc
     1621 gctcggcgct cgtgttgagg atgacttcga cctcgactcc cgtctctggc acatcaccgt
     1681 cgttctccac ggtaacggcg aaggtcggct cgtccgctcc tgtcaggaca acgttcccgc
     1741 ccgagtacat ctgcttcccc gcgaccttga cggccgttac ccgcaccccg tgcagggcgt
     1801 tcgggtcgct ccttgcgcta ccagatacgg gggccgtgtc gaaaccgagc gccccgtaat
     1861 ccataaagga ttgcggttct tcgaggtagg tttggtcgct tgtctggccc acagtctcca
     1921 gggaatgctt gacggccggg atgtagtagt tcgcgacgat gctgtcgctt gttctgtagt
     1981 cctcgacggc cgtcgcgagc tccccggcaa acccgctcgc atcccccgta gccgcctgca
     2041 ctagtttgtc agtggcctgg gcccttatgc ccagcgctgt gacgacgtag tgatgggcgt
     2101 cctcgaactc tggaggtatc tcctcctgcc tcaacgcctg tacgtattgc tgttccgtac
     2161 ggttggccgc ctgggcgagc gcctcctcgt ccagcctggc agggttccct ccggcgttgc
     2221 ccaggatgcg ctggagttcc cctctgcccg cgttggcgga gtcgctcaag aagctgtccg
     2281 cgctcgtgac gtacttgcgg acctgggtcg actgctgggt tgccacgcag ccgcgggcga
     2341 cgaagaagat aaacccgaag accaccacta ggaggaggat ggagccgacg aaggtgagcc
     2401 aggcagggag cgtcctcctg cggcgctctt tcggaccgct gcggggtctc ctgggacgac
     2461 ccgacggcct ctgccggcgg aggtcggggg cggccctgtg agatctacgc tctatacgaa
     2521 cccccaaaga acatgtcgtg ggcgaggggg gactcgaacc cccacggcct ctcggccact
     2581 agaccctgaa cctagcgcgt ctaccaattc cgccactcgc ccgcaggccc tctccaggaa
     2641 acgtcctgaa ctgcggggga ttgtaccagc atttcagcaa ggcgccgtta gcaccttgct
     2701 gacaggcggt ttgcgatgca aaccgcctgt cataggctat acaatagcct atcaagtagt
     2761 gacagagacg cgctacatcg aaggacaggg acgctacgcg ctggccgaga tggtcgggcg
     2821 cggcggcttc gcgaccgtgt ggcgcgcccg ttctctcaag ggccacctca ggggcaaaga
     2881 cgtggccgtc aaggttatcc ccgtttacag tgccggcgag cgctcgcgcg cccttaggga
     2941 gggccagatc gccgaagggc tcaggcacaa gaacatagtg gagaccctcg aggtcatccc
     3001 cggcgaccat gagatctacc tcgtaacgga gtatgtgcgg gggatgccgc ttgatgaggc
     3061 ggccaaaggg tacgacgtgg aggagatcgt ggattcgttg gcccagatcc tcgaagccct
     3121 ggtctacgcc cacgggcagg gcataatcca ccgcgacata aagccgcaga acgcgctcgt
     3181 ggacgcccgt gggcgggtaa agttgacgga cttcggggtc gcgttcaggg cgggggacac
     3241 gcgtcttacg cgcgtcggct tcgccgtggg cacgcctggc tacatcgcgc cggagatcct
     3301 ggacggcgcc gacccgaccg cgctcacgga catctacgcc gtcggcgcca cggcccgtac
     3361 cctgctcgcg agccagccct acgagccacc cccccgcctc agggaattcg tcgacaggac
     3421 cacctcgccc aacccggacc accgtccgca gagcgcgtgg gccgccgtca agctccttac
     3481 gggcaggaag gccccggcgg gcagcatctc gccaggtgga cgcaaggtgc ccgacaggct
     3541 ctcgggcggc ttcaaagacg gtgcgttgcg catcatcaac ggcgtagccg cagggtgcct
     3601 cggttacctc ctcgcggccc agatcctcgc ggacggggcg caggcgcttg ggatcgccgc
     3661 cgggctcggt gtgctggcct acctgctgcc gcgcctgggc gccctcggtg tcatagtcgc
     3721 tctggccgtt ctgctgatac ggaatggggc cggcctcggg ttctctgcgc tcttgcccgt
     3781 cctcgggggc gtctgggtgc tgggcgccgg ttcggccaat ggcgatgtga gaaggctacc
     3841 gcttgggcct gcgctcgcca tgccgctggc tctcctc
//
LOCUS       DATMJA010000355         2828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_877633, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000355 DATMJA010000000
VERSION     DATMJA010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877633"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VNT12_08555"
     CDS             complement(<1..1157)
                     /locus_tag="VNT12_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HWI46208.1"
                     /translation="MTATANLSLVPSMGEARRLARSYDVVPLYAEFIGDLETPISAVL
                     RFAEEDTVFLLESAESAERFGRYSFLGFDPKRTLSYRDGLYTVVDADGVREVPAGDPF
                     RGLAEIVGKKSVAPLPNLPAFVGGAVGYFSYDAVRYLERLPDAPADDLAVPEACFAVT
                     DTLIVFDHLRHKVLVVSLVDAAGLRKVEGDGFSAAYRRAADDIRRIAERLSAPLVRRM
                     PAFGPGVEISSNFTREGYEEAVEAAKEYIRAGDAFQIVPSQRFAAEIGDLDPLLLYRG
                     LRTVNPSPYMTYLKLGGLTIVGASPEPLVRVEGRRVMTRPVAGTRGRGSTPEEDAALA
                     EELLADEKERAEHVMLVDLGRNDLGRVSEVGSVGLESFMEIERYSHVMHIVS"
     gene            complement(1141..1776)
                     /gene="hisIE"
                     /locus_tag="VNT12_08560"
     CDS             complement(1141..1776)
                     /gene="hisIE"
                     /locus_tag="VNT12_08560"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HWI46209.1"
                     /translation="MISAPDRVKFDDNGLVPVIAQDVATGDVLTLAYANREAVEKTLS
                     SGEAYYYSRSRSELWRKGATSGNTQRVVEVKLDCDEDALLYRVQPRGPACHTGQNSCF
                     FTTLAGERVGAADDREVPFGTMVERLAGTIARRHREMPEGSYTASLIQGGKKRLAQKV
                     GEEAVEVVVAALSDERLSEEAADLVYHLLVLLEERGVGTEDVAKVLHDRHG"
     gene            complement(1787..2587)
                     /gene="hisF"
                     /locus_tag="VNT12_08565"
     CDS             complement(1787..2587)
                     /gene="hisF"
                     /locus_tag="VNT12_08565"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWI46210.1"
                     /translation="MSLKVRIIPCLDVDAGRVVKGTNFTNLRDAGDPVELAALYDREG
                     ADEIVFYDITASHEQRETAAALARAAAEEVFVPYTIGGGVRTVGDMRAMLRAGADKVS
                     INSAAVRNPYLITEGAERFGSQCVVLSVDVKRRGLSPGWEVYLNGGRVNTGMDAVGWL
                     VEGERRGAGEFVLNSMDADGTETGYDLDLISAVAEKTTLPIVASGGAGSPEHMIAAVN
                     AGAGAVLAASIFHFGEYSIAEVKEHMMQAGIPVRFVDQGSDVVSGAEG"
BASE COUNT          461 a          969 c          868 g          530 t
ORIGIN      
        1 gagacgatgt gcatgacgtg cgagtagcgc tctatctcca taaagctctc caacccgacc
       61 gagccgacct cgctgacgcg cccgaggtcg ttgcgcccga ggtccacgag catgacgtgc
      121 tcggcccgct ctttctcgtc ggcgagcagt tcttcggcca gggcggcgtc ttcctccggc
      181 gtgctgccgc gcccacgggt gccggctaca ggacgggtca tcacgcgtct tccctcgacc
      241 cgcaccaggg gttcggggga tgcgcccact atggtgaggc cgccgagctt gaggtaggtc
      301 atataggggg atgggtttac cgtgcgcagg ccgcggtaga ggaggagagg gtcaagatcc
      361 cctatctcgg cggcgaacct ctgggaaggg acgatctgaa aggcgtcccc agccctgatg
      421 tactctttcg ccgcctctac ggcctcctcg tagccctctc tggtgaagtt cgaggagatc
      481 tcgaccccgg ggccgaacgc gggcatccgg cgcaccagcg gggcggagag gcgctccgcg
      541 atcctgcgta tgtcgtcggc cgcccgcctg taggccgccg aaaagccatc accctctact
      601 ttgcgcaggc ccgctgcgtc gaccagtgag acgacgagca ccttgtgcct gaggtggtcg
      661 aagacgatca gggtgtccgt aacggcgaag caggcctcgg ggacggcaag atcgtctgct
      721 ggggcgtcgg gaaggcgttc gagataacgc acggcgtcgt aggagaagta ccccacggcg
      781 ccgcccacga acgccgggag gttgggaagc ggagctacgc tcttcttgcc cactatctca
      841 gccagtcccc tgaaggggtc gcctgctgga acctcccgca caccgtctgc gtcgacgacc
      901 gtgtagaggc cgtccctgta ggagagcgtg cgtttggggt cgaatccgag gaaggagtag
      961 cggccgaacc tctcggccga ctcagcgctc tccaggagga ataccgtgtc ctcctccgcg
     1021 aacctgagga cggccgagat cggcgtctcg aggtctccga taaactcggc gtacagcggt
     1081 accacgtcgt aggaacgggc gagtctgcgg gcctcgccca tcgaagggac caggctgagg
     1141 ttagccgtgg cggtcatgca gtaccttcgc tacatcctca gtgcccacgc cccgctcctc
     1201 gagtagcacc agcaggtggt agacgaggtc agccgcctcc tccgatagcc tctcatcgga
     1261 gagcgcggca accacgacct ccaccgcctc ctcccccacc ttctgggcca gccgcttctt
     1321 tccgccctgt atcagggagg ctgtgtatga tccttcgggc atctccctgt gacgccgcgc
     1381 tatcgtaccc gccagccgct cgaccatcgt cccgaaaggg acttccctat cgtctgcggc
     1441 cccgactctt tcccctgcca gggtggtgaa gaagcagctg ttctggcctg tatggcaggc
     1501 tggcccgcgc ggctggactc tatagaggag tgcgtcttcg tcgcagtcca gcttcacctc
     1561 gactaccctc tgcgtgttgc cactcgtcgc gcccttgcgc cagagctcgg agcgcgagcg
     1621 ggaatagtaa taggcctcgc cgctggagag cgtcttctcg acggcctcgc ggttggcgta
     1681 ggccagcgta agcacgtcgc ctgtcgccac atcctgggcg atgacaggca ccagaccgtt
     1741 atcgtcgaac tttactctat ctggcgctga gatcatcctt ctatcatcat ccctccgcgc
     1801 cgctaaccac gtccgacccc tggtcaacga accggaccgg gatgccagcc tgcatcatgt
     1861 gctccttgac ctcggctatg ctgtactccc caaagtggaa gatgctcgcg gcgagcaccg
     1921 cgcctgctcc ggcgttcacg gctgcgatca tgtgctccgg actccccgcc ccaccggacg
     1981 cgactatggg gagggtcgtc ttctcggcga cggcggagat aaggtcgagg tcatatccgg
     2041 tctccgtccc gtctgcatcc atgctgttga gcacgaactc ccccgccccc cgcctctctc
     2101 cttcgactag ccaccctacg gcgtccatcc ccgtgttgac ccgcccgccg ttcaggtaga
     2161 cctcccagcc aggggagagg ccgcgccgct tcacgtccac gctcaacacg acgcactggc
     2221 tcccgaagcg ttccgctcct tcggtgatga gatacgggtt ccgaacggcg gcgctgttta
     2281 tggagacctt gtcagccccc gcgcgcagca tggcgcgcat gtcgccgacc gtccttacgc
     2341 cgcctccgat cgtgtatggt acgaagacct cctcggcagc agcgcgggca agagcggcgg
     2401 ccgtttccct ctgttcgtgg gaggcggtga tatcgtagaa gacgatctca tctgccccct
     2461 ccctgtcgta gagggccgcg agctccaccg ggtctccggc gtccctcagg ttggtgaagt
     2521 tcgtcccctt taccacccgc ccggcgtcca cgtcgaggca gggtatgatg cgtaccttga
     2581 ggctcaccgg gggctcttct cccgcctgcg cccaagacga gccaggcgct ccccaaagag
     2641 ggcgcgtatg tggcgctgcg ccgatggcag ggccacgttc aacgaaacga ccttcaccgc
     2701 ttttccggct ccttcctggt agctcattac agggtattgt acgaggctac acgtagggca
     2761 caccggcgtt gccgcattct tcggccaggc gctcgcgcag ggcgttcctc gctggtagca
     2821 gaacggag
//
LOCUS       DATMJA010000356         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_285511, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000356 DATMJA010000000
VERSION     DATMJA010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285511"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /gene="pheT"
                     /locus_tag="VNT12_08570"
     CDS             complement(<1..1266)
                     /gene="pheT"
                     /locus_tag="VNT12_08570"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWI46211.1"
                     /translation="MRVPLSWLHEYVDFDLSADELVDLFSLRSQEIDGVYRLGVTGGE
                     VVVGEVIEFGPHPNADRLFVAKVDLGGREVQIVAGASNPYPGARVPVVLPGSMMSDGT
                     KLREAKLRGLQSYGMMMSERELGISQDHEGILLLDDSYEVGRPVAEYFPVGETVLDID
                     VTPNRPDLWGMIGVARELAAILGTEYRVPEPTFETGGKPTHDFGLRVEAEDLCPRYDL
                     RRISDLQAGQEAPLWMRRKIYAAGMRPINAIVDATNYVMLETGQPIHAFDAAMVREGI
                     VVRRGRPQEKITLLDGSTRSLDEETLVIADEERGLVIAGIMGAEDAEVGEETTDALIE
                     VANFEGRNILQTSQKLGLRTDASGRFERGLDPNLVAYAIDRVSALLTQAPGGRVADDT
                     LSHYPEPVEPWRVPLRLSRAELLLGMPVPE"
     gene            complement(1279..2307)
                     /gene="pheS"
                     /locus_tag="VNT12_08575"
     CDS             complement(1279..2307)
                     /gene="pheS"
                     /locus_tag="VNT12_08575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWI46212.1"
                     /translation="MTVSERIEQLKSEALSAVSSSASTADLEDIRVRFLGRSAELTEI
                     KKGIGTLSPEERKEIGRSSNLASRDIEAALDSRTEEVAAREQEERLRAEAVDVTLPGV
                     PFQRGHLHPSQMVIDDVVNFFIGLGYRVAEGPEVETDYYNFTALGIPPGHPARTMQDT
                     FFVDDGLVLRTQTSPMQIRTMLAQEPPVYVVCPGRTYRRDSDPTHTPMFHQVEGLAVD
                     RGLTLGHLKGTLAAMARHIFGDDVEIRLRPSYFQFTEPSVEMDMSCFACGGGDPNCKV
                     CKRAGWLEMLGAGMVDPGVLEEVGYDSEKYTGYAFGMGPDRMAMVKYGVPDLRLFFEG
                     DLRFLRQF"
     gene            complement(2317..>2647)
                     /locus_tag="VNT12_08580"
     CDS             complement(2317..>2647)
                     /locus_tag="VNT12_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWI46213.1"
                     /translation="LYNPKTVRASMGSIFHTPVARELESVDFLAEAGSAGFGLVAAVP
                     EAGRKPAALPGGRLVVCIGAEGSGLPEEVVGACEMEVGIPSQAPSLNASVAASILLYE
                     AYSRVLP"
BASE COUNT          494 a          866 c          795 g          492 t
ORIGIN      
        1 ctctggcacg ggcatgccga gcaggagctc ggcccggctg agccgcagcg gtacacgcca
       61 gggctccact ggctcggggt agtggctcag ggtgtcatcg gcgacccttc caccgggagc
      121 ttgcgtgagt agggcactca cccggtctat cgcgtacgcc accaaattcg ggtcgaggcc
      181 gcgttcgaag cggccggagg cgtcggttcg caggccgagc ttctgggagg tctggagaat
      241 gttgcgccct tcgaagttgg ctacctcgat aagggcgtcc gtggtctcct cccccacctc
      301 agcgtcctcg gcgcccataa ttccggcgat cacgagccca cgctcctcgt cggcgatcac
      361 gagcgtctcc tcatcgagcg agcgcgtgga accatcgaga agtgtgatct tctcctgggg
      421 cctcccacgc ctgacgacga tgccttcacg caccatggca gcgtcgaagg cgtggatggg
      481 ctggcccgtc tccagcatga cgtagttggt cgcgtctaca atagcgttaa tgggccgcat
      541 gccggcggcg tagatcttgc gccgcatcca gagcggcgcc tcctgccccg cttggaggtc
      601 agagatgcgc ctgagatcgt atcgcggaca caaatcctcg gcctcgaccc tgagtccgaa
      661 gtcgtgggtc ggcttccctc ctgtttcgaa agttggttca gggaccctgt attccgtccc
      721 aaggatggcg gccagctccc tggccacccc gatcataccc cacaagtcag gcctgttcgg
      781 ggtaacgtcg atgtccagaa ccgtttcccc gacgggaaag tactcggcga cgggacgccc
      841 tacctcgtac gaatcgtcga gcaggagtat cccttcatga tcctgtgaga ttccgagctc
      901 gcgctcgctc atcatcatcc cgtaggattg caggccgcgc agtttcgcct ctctaagctt
      961 cgtcccgtcg gacatcatgc tgcctggcag cacgacgggc actcgggcgc cggggtaagg
     1021 gttcgaggcg ccggccacga tctgcacctc cctgcccccg aggtcgacct tcgcgacaaa
     1081 gagcctgtcg gcattgggat gcggcccgaa ctcgattacc tcgcccacga ctacctcacc
     1141 gcctgtcaca ccgaggcggt agacgccatc gatctcctgg cttcgtaggg agaagagatc
     1201 tacgagctca tcggcggaga ggtcgaagtc gacgtactcg tgtagccagc ttagcggaac
     1261 gcgcaaggtg aaccccttct agaactgacg cagaaagcgg aggtcgccct cgaagaacag
     1321 acgaagatct gggacaccat acttgaccat cgccatcctg tcagggccca tcccgaaggc
     1381 gtagccggtg tacttttcag agtcgtagcc gacctcctcg agcacaccgg gatccaccat
     1441 cccggccccc agcatctcga gccaacccgc ccttttgcag accttgcagt tcgggtcccc
     1501 gccgccgcag gcaaagcagc tcatgtccat ttccacgctc ggctcggtga actggaagta
     1561 gctcggacga agccggatct cgacatcatc cccgaagatg tgacgggcca tcgcggcgag
     1621 cgtccccttc aggtggccaa gcgtgaggcc cctgtccacg gcgagacctt caacctggtg
     1681 gaacatcggc gtatgcgtcg ggtcggagtc gcgcctgtac gtgcgtcccg gacacactac
     1741 atagacaggc ggctcctgtg cgagcatcgt cctgatctgc atcggcgacg tctgcgtacg
     1801 caaaaccagc ccatcatcca cgaagaacgt gtcctgcata gtacgtgcag gatgccccgg
     1861 cggtatcccc agggccgtga agttatagta gtcagtctcg acctcgggcc cctcggcgac
     1921 gcgatagccc aggccgataa agaagttgac aacgtcatct attaccatct gggatggatg
     1981 caggtggccc ctctggaacg ggacaccagg cagcgtaacg tcgacagctt cagcccgcag
     2041 acgctcctcc tgttcacgcg ccgcgacctc ttcggtcctg gagtcgaggg ccgcttcgat
     2101 gtcacgcgag gcgaggttgg aggacctgcc gatctccttg cgctcctcgg gagatagtgt
     2161 acctatgccc ttctttatct ccgtaagctc cgcggagcgt ccgagaaatc gtacgcggat
     2221 gtcttcgaga tccgcggtag aagcgctgga ggagacggcc gagagtgcct cggactttaa
     2281 ctgttcgatg cgctcgctca ccgtcattgt gggcgattat ggtagcacac gcgaatacgc
     2341 ctcgtagagc aggatcgacg ccgccacaga tgcgttcaac gagggcgcct gcgacggtat
     2401 accgacctcc atctcgcagg ctccgacgac ctcttcaggc agccctgaac cctcagcgcc
     2461 gatacagact acgagtctgc cgccgggaag agccgctggc tttcgtcctg cctcggggac
     2521 ggccgcgacg agaccgaaac cggcggagcc tgcctcggcc aggaagtcga cggactcgag
     2581 ttcccgcgcc acaggggtgt ggaagatgga ccccatcgac gccctgaccg tctttggatt
     2641 gtagagg
//
LOCUS       DATMJA010000357         3298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_167274, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000357 DATMJA010000000
VERSION     DATMJA010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167274"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1428
                     /locus_tag="VNT12_08585"
     CDS             79..1428
                     /locus_tag="VNT12_08585"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWI46214.1"
                     /translation="MDLRERVYGSREELLGELYEFLRMPSISAREGESRDFRNCAQWV
                     AGKLEDAGAEARIMETEGHPVVYAEVGTGKPTLLSYGHYDVQPPEPLDLWESEPFEPT
                     IRDGGLYARGVADDKGDVLARIQALRLYMEEHGPPPFKLKFLIEGEEEVGSPSLAPFV
                     RSNADLLSADACLWEGSMKDEAGRPMIFCGTKGMAYVELRARGASHDLHSMYGGIAPN
                     PVWRLIQALRTIKDENGEITLDGLDALAEEPSEKDLEAISRIPFHDAALRESWGVEAL
                     DRSLTGQEALHEMLVRPTANIAGIQSGYTGPGSKTIVPSEAFVKMDFRLVAGQSPGPV
                     VELIRSHLRKRGFDDVEVVDLHGVEPAKTPIDSPVVRTALQTWQDLGVDEAVVYPTIG
                     GSGPTSLLVTDLRIPTIMTGSVANVGSRLHSPNEWVRLDDYFEGIRYFARFFERFAL"
     gene            1425..2009
                     /locus_tag="VNT12_08590"
     CDS             1425..2009
                     /locus_tag="VNT12_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46215.1"
                     /translation="MTEKKGRGLDLPERVRQSLAGAVADRGVQARVISTQGRESMRPP
                     QGHIIIAAGESEVEAVRGPARIVRRLEFYKTFYGPVVRLALSVYPIEGKPLSAGTALH
                     VSQVSGDAALTGLGRQKSIYIHFYSADEGDLTYAFSKEIPNAQEQRGETKKVLKMARD
                     AFAETPEERRSFRSAVGLAERQFELPVPEPDAEG"
     gene            2043..3182
                     /locus_tag="VNT12_08595"
     CDS             2043..3182
                     /locus_tag="VNT12_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46216.1"
                     /translation="MQAGNAVEDFVEKISQALEGAWRRGIHVTAGAGSCDLEAQQFGG
                     LPEEEESFPTGRPHHLESPVGSRDLGGHDGTSQLCYFLDGVQSSREIGRIGMSPVIVS
                     TVAAAIVNRCGRRFSRMPLESPPAVVQAVILPRSAGDASVEAFWDLLLAAGFSELDPD
                     EVPSSPHLVLDSAEYIADADPSDYVGMRERARGRVRSLRERLEGGLLRRWEGDDRVLE
                     DSEAWIAVDGQLKDIRESNRRAIGLIKSVARPEFVGKDIGMLLDLGPGMRTTSFIPDW
                     QLRRDQGERRTSWYLRMWPPQPGADALGSLMRVEAPRDTEPEQVDEISRWILAERAPL
                     AKPDPRWPAMIYPIQYVEKILKPLVQGPERAYARLERQLASNGRN"
BASE COUNT          643 a         1047 c         1080 g          528 t
ORIGIN      
        1 cataagacac gaagtgggga cgcgaaggcc gggcgcgaaa gtgctaacct catcgcaggc
       61 acaaaggagg tctgctgaat ggatttgcgt gagcgcgtgt acggttcgag agaagagtta
      121 ctcggggagt tgtacgagtt cctcaggatg ccttcgatct ccgcccgcga gggcgagagc
      181 cgagacttcc gcaactgcgc acagtgggtt gcggggaagc tggaagatgc cggcgcagag
      241 gcccgcatca tggagaccga aggacacccc gtggtctacg cggaggtcgg aacgggcaaa
      301 ccgacgcttc tctcttacgg ccactacgac gtgcagccgc ccgagcctct ggatctctgg
      361 gagagcgagc cgttcgagcc gacgatcagg gacgggggcc tctacgcccg cggcgtcgcc
      421 gacgacaaag gcgacgtgct cgcccggata caggctttgc gtctatacat ggaggagcat
      481 ggcccgccgc ccttcaagct caagttcctc atcgagggcg aggaggaggt cggcagcccc
      541 agcctcgctc ccttcgtccg ttccaacgcc gatctcctct ccgccgacgc ctgcctctgg
      601 gagggctcca tgaaagacga ggcggggcga cccatgatct tctgcggcac caaagggatg
      661 gcctacgtgg aactgcgcgc ccgcggcgcc tcccacgacc tgcacagcat gtacggtggc
      721 atagccccga acccggtgtg gcgcctcatc caggccttga ggaccatcaa ggacgagaac
      781 ggtgagatca cgctggacgg cctcgacgcc ctggccgaag aaccctcgga gaaggatctc
      841 gaggccataa gccgcatccc gttccacgac gccgcactgc gggagtcttg gggcgtcgag
      901 gccctggacc gcagtctgac cggccaggaa gcgctgcacg agatgctggt gcgcccgacc
      961 gcgaacatcg cgggcataca gtctggttac accggtcctg gctcgaagac catcgtacct
     1021 tcggaggcgt tcgtaaagat ggacttcagg ctcgtcgcgg gccagagccc cggtcctgta
     1081 gtcgagttga tccggtccca cttgagaaag cgcggtttcg atgatgtcga ggttgtcgac
     1141 ctgcacggcg tcgagccggc gaagacgccc attgactcac ccgtagtccg aaccgcactg
     1201 cagacgtggc aggatctcgg cgtcgacgag gcggtcgtgt atccgaccat cgggggaagc
     1261 ggaccgacct ccctcctggt caccgacctc agaataccga ccatcatgac cgggagcgtg
     1321 gctaacgtag ggagccggct ccactccccg aacgagtggg tccgcctcga cgactacttc
     1381 gagggcatca gatacttcgc ccgcttcttc gagaggttcg ccctttgacc gagaagaagg
     1441 gtagaggtct cgacctgccc gaacgcgtcc gtcagagcct cgccggagcg gtcgcagaca
     1501 ggggcgtgca ggcgcgcgtg atctccacgc agggccgcga gtccatgcgt cccccgcaag
     1561 gacacataat catcgccgcc ggagagagtg aggtggaagc ggtccgcggc cctgcccgca
     1621 tagtacgccg gctggagttc tacaagacgt tctacgggcc cgtcgtccgc ctcgccctca
     1681 gcgtctaccc gatagagggc aaaccgctct ccgccggcac cgccctccac gtctcccagg
     1741 tcagcggtga tgcggccctc acgggactcg gccgccagaa gagcatctac atacacttct
     1801 actccgccga tgaaggcgac ctcacctacg ctttctccaa agagatcccc aacgcccagg
     1861 agcaacgcgg cgagacgaag aaggtcctca agatggcccg cgacgccttt gcggagacac
     1921 cggaggagcg caggagcttc agatctgccg taggactcgc cgagcggcag ttcgagcttc
     1981 ctgtacctga acctgacgca gaaggatagt cttctgcggc gagcccgcgt atactgccac
     2041 tcatgcaggc ggggaacgcg gtagaagact ttgtagaaaa gataagccag gccctcgaag
     2101 gggcttggcg cagaggcatc cacgtaaccg ccggagcggg ttcatgcgat ctggaggccc
     2161 agcagttcgg cggccttcct gaggaagaag agtcgttccc taccgggcgt ccgcaccatc
     2221 tggagtcccc ggtgggatcc agggacctcg gtggccacga cggcacctcg cagctttgct
     2281 atttcctaga tggggtacag agctcgcggg agatcgggcg gatagggatg tccccggtga
     2341 tcgtatcgac ggtggcggcc gcgatcgtca accgctgcgg gaggcggttc tcacggatgc
     2401 cgctggagag cccgcccgca gtggtgcagg ccgtgatcct accccgcagc gccggcgacg
     2461 cgagcgtcga agcgttctgg gatctgctgc tcgcagcagg attctccgaa ctcgaccctg
     2521 acgaggtccc ctcctccccc cacctcgtcc tcgactccgc ggagtatata gccgacgcag
     2581 acccgtccga ctatgtaggt atgagagaga gggcgcgcgg aagagtcagg tcgctaaggg
     2641 agcggctgga gggaggactg ctcaggcggt gggagggtga tgacagggtt ctcgaggatt
     2701 ccgaagcctg gatcgcggtt gacggacagc tcaaggacat cagggaatcc aacagacgcg
     2761 ccatcggcct cataaagagc gtggcccgtc ctgagttcgt tggaaaggac atcgggatgc
     2821 tgctcgacct ggggcctggg atgcggacga cgagcttcat ccctgactgg cagcttcgtc
     2881 gcgaccaggg cgagcggcgc acctcctggt acctcaggat gtggcctccg cagcccggcg
     2941 ccgacgccct cggctcgctc atgcgcgtcg aagcgccgcg cgacaccgaa ccagagcagg
     3001 tcgacgagat atcgcgctgg atcctcgccg agcgggcgcc cttggcgaag cccgacccga
     3061 ggtggccggc gatgatctac ccgatccagt acgtcgagaa gatcctgaag ccgctggtgc
     3121 agggcccaga gagggcctac gcacggctgg agcgccaact cgcatcgaac gggaggaact
     3181 agatgctgca cgatagtttt ctggaccaag cctcgagccc gataggccgg gtcgttacca
     3241 gtgaggagag cccggccacg gcccatgagt tcttcttctg gaccgcagag accgaagc
//
LOCUS       DATMJA010000358         3219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_404021, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000358 DATMJA010000000
VERSION     DATMJA010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404021"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VNT12_08600"
     CDS             complement(<1..749)
                     /locus_tag="VNT12_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HWI46217.1"
                     /translation="MRARRFSQVARCLGTSALVLLVLALASCGNSETKSAPSSDGAVS
                     VGRETAGVGSPAGSGPDLAQSSEGAAGITEDPASLGEFGRKVVKTADLGLRSEEVRQS
                     AARAQQVAATAGGTVLSSQVYRSDDSITADLVLSVPSEEFERVLDELRSLGEKVTTDS
                     ISGQDVTEEYVDLKSRERNLRATEESLLRLYDRAENVEEALSIQRELTGVRGEIELVQ
                     GRIKYLDQRSTYPQITLNIQPVTSPPPPRPT"
     gene            complement(866..1654)
                     /locus_tag="VNT12_08605"
     CDS             complement(866..1654)
                     /locus_tag="VNT12_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46218.1"
                     /translation="MPDRELERELRELGALIDYPPTPDVARAARNLLDEEDSRPQRFL
                     RMPFPTMRWAAVAAAFVLVVAVPTLSPSLRATISDWFVAEHFQSAGGHAVDAGSPERQ
                     SETGAPAAGVSKSGTSTALAPPFSGERISLREARARMNGALLLPRTTKLGKPEEIYAG
                     GTSRKDGVRLVYSEGLPPLGDTGISLVLTEIPNDLEPAYLRGKTTVKSELERVSVDGG
                     PGYWSAAGHRLPGHVLLWEQGGVALRLEADVQKEQAIRIAESVR"
     gene            complement(1647..2174)
                     /locus_tag="VNT12_08610"
                     /pseudo
     CDS             complement(1647..2174)
                     /locus_tag="VNT12_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            2401..>3219
                     /locus_tag="VNT12_08615"
     CDS             2401..>3219
                     /locus_tag="VNT12_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWI46219.1"
                     /translation="MQDERLGENTTAGKLEGLGHLREAAAHPAPEQAVERQRERGKLT
                     ARERIELLLDTGTFVELDRYRVHRSYNFGLEENRPLGDGVITGYGEVSGRKVCVFSQD
                     FTVFGGSLGEVYAQKICKVMDLAISTGCPIIGINDSGGARIQEGVVSLAGYADIFHRN
                     VLASGVVPQISVIVGPCAGGAVYSPAITDFVFMVEETSHMFITGPDVIKSVTGEDVSF
                     EDLGGAATHNTRSGVAHFSAPDEETCFDDVRYLLSFLPENNLESAPYFRPTDDPE"
BASE COUNT          519 a         1083 c          999 g          618 t
ORIGIN      
        1 caggtaggcc ggggtggcgg cgggctcgtg acgggttgga tgttcagggt gatctggggg
       61 taggtgctgc gctgatccag gtactttatc ctgccctgca ctagttcgat ctccccgcgg
      121 acgcccgtca actcgcgctg gatcgagagc gcctcctcga cgttctcggc cctgtcgtag
      181 agcctcagca ggctctcttc ggtggccctg aggttacgct cgcgggactt gagatctacg
      241 tattcctcgg tcacatcttg cccgctgatg gagtccgtcg tgaccttctc gcccaggctc
      301 ctcaactcgt cgagcaccct ctcgaactcc tcggagggga ccgagagtac gagatccgcc
      361 gtaatggagt cgtcgctcct gtagacctgc gagctgagta cagtgccacc ggcggtcgcg
      421 gcgacctgct gcgcccgcgc ggcgctttgg cggacctcct ccgagcggag gccgagatcg
      481 gcggtcttga cgaccttgcg gccgaattcc ccgagagaag caggatcttc ggttatgccg
      541 gcagcaccct cggaggactg cgcaagatcc ggtccagacc cggccggtga gcccacacct
      601 gccgtctccc gccctaccga caccgcgcca tcggacgatg gcgccgattt cgtctcgctg
      661 ttcccgcacg acgcgagagc cagaacgagg agcacgagcg cgctcgtccc caggcacctt
      721 gccacctgtg aaaaacgtct ggctctcatt gcatccacct ctttcggacg tgtacctccc
      781 gcggagcacc ttctggctcc gaagggcccg tctacggccc cacttactcc ctgtacactg
      841 ccagcccccg atcggttccc cgttttcaac gaaccgattc ggcgatgcgg atcgcctgct
      901 ccttttgcac gtccgcttcg agccggagcg ctacgcctcc ttgctcccag agcaggacat
      961 ggccggggag gcggtgtccg gcagcgctcc agtatcccgg acctccgtcc acgctcaccc
     1021 tctctagctc cgacttgaca gttgtctttc cacggagata cgccggttcg agatcgtttg
     1081 ggatctcggt caggacgagg ctgatcccgg tgtctccgag gggcggcaac ccttcgctgt
     1141 agacgagtct gacgccgtcc ttcctggatg tcccgcccgc gtagatctcc tctggctttc
     1201 ctaacttcgt cgtccggggc aggaggagcg cgccattcat tctggcccgc gcctcacgca
     1261 ggctgatacg ctcgcccgag aacggtggag ctagcgcggt ggatgtgcca cttttggaga
     1321 cgccagcggc gggtgcgccc gtctccgact gtctctcggg tgagccggca tcgacagcgt
     1381 gcccaccggc gctttggaag tgctctgcta cgaaccagtc gctgattgtg gcacgcagac
     1441 ttggtgagag ggttgggacg gcgacgacga gcacgaacgc cgccgctacc gccgcccaac
     1501 gcatcgttgg aaagggcata cggaggaacc tttggggccg gctgtcctct tcatcgagca
     1561 ggttgcgggc ggcgcgcgcg acgtccggcg tcggaggata gtcgatgagg gccccgagct
     1621 ccctcagctc tctctcgagt tctctatcag gcatctccct ccttcgcaag ctcggtgcgc
     1681 aagctcttga gcgcccgcga gagccgcgac ttgacggttc ctcgcgcgca acccaggatc
     1741 gcggccgtct cttcttcgga gaggcccagg aggtagcggc agccaatggc ccaccgcgcc
     1801 tcctcacgca ggctgcccag ggcctcgagc aactcttctc gccgctccgc cgccagaacc
     1861 gtcgcctcag gggaggaagc gtaattctca ggcgtgcctt cccgcgtcgc ccgcaggaac
     1921 aggccggcgc gacgcgcggc cgccctgcgc tggtttctgg cctcgttggc gactatcgcg
     1981 aacagccagg gcctgaaacg ggcgcccgat ctgaagcgtc ccagggcgcg gtacgccttc
     2041 acgaaggcct cctgggtcgc ttcctcggcc tccgaggcgt cgcctgtgat gaggtaagcg
     2101 gtgcggaagg ccacctcctg gtagccgcgc accagcctct catacgccgc agtgtctcca
     2161 cccttcgccc gctctacgag cttcgcgtca tccgaggtga cggccctcct cctcgacgtc
     2221 gctctctacc tccatctaca acgccgggcg caaaaaggtt cccggcgtga tggctaatga
     2281 tgacgcctca gtcgagtctc tgcgagtgcc ccagggtgta gaaaagttct ttgcgtctgg
     2341 ggttttcgtg atctgcgcgc cgggaaaccg ccatttccct gcgtgctaga atgcctgccc
     2401 atgcaggacg aacgactcgg ggaaaatacc acagcaggga agttagaggg tctggggcat
     2461 ctgcgagagg ctgcggccca tcctgccccc gagcaggccg tcgagaggca gcgagaacgc
     2521 ggcaagctga cggcacgcga gcgcatcgag ctcttgctcg acactggcac gttcgtcgag
     2581 ctggaccgct acagggtgca ccgctcttac aacttcggcc tggaggagaa caggcccctc
     2641 ggggatgggg tcatcacggg ctacggtgag gtttcggggc gcaaggtctg cgtcttttcc
     2701 caggacttca ccgtgttcgg gggttccctg ggcgaagtct acgcgcagaa gatctgcaag
     2761 gtcatggacc tcgccatctc gacgggttgt cccattatcg ggatcaacga ctcgggaggg
     2821 gcgcgcattc aggagggtgt cgtctcgctg gccggctacg ccgacatctt ccaccgtaac
     2881 gtgctcgcct cgggtgtagt gccccagatc tcggtgatcg tcggaccctg cgcgggcggt
     2941 gccgtatact cgccggcgat cacggatttc gtcttcatgg tcgaggagac gagccacatg
     3001 ttcatcacgg gccccgacgt cataaagagt gtcaccggcg aggacgtctc cttcgaggac
     3061 ctcggtggcg cggccaccca caataccagg tctggcgtag cccacttctc ggcacccgat
     3121 gaggagacct gcttcgacga cgtgcgctac ctgctatcct tcctgcccga gaacaatctg
     3181 gaaagcgccc cttacttcag gccgactgat gacccggag
//
LOCUS       DATMJA010000359         2340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_760113, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000359 DATMJA010000000
VERSION     DATMJA010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760113"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VNT12_08620"
     CDS             complement(<1..1040)
                     /locus_tag="VNT12_08620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWI46220.1"
                     /translation="MPKPVGSSQRYMPGLDGLRAIAVLAVIAYHLNPAWAPGGLLGVG
                     VFFTLSGYLITDLLLGQRAAVGSLRLGDFWLRRARRLLPALFLMLAVVVAWVTLLDRS
                     LLPGLRGDVLAAVGYVSNWWNILREASYFARFGPPPPLDHLWSLAVEEQFYLIWPWLL
                     WLGLRYVPWRYTLAGLTLAGAALSAAAMALIYEPGVDPTRVYEGTDTRASGLLIGAAL
                     AMVWPSRNLRADLAVRRRLLLNGAGVAGLAVIALLIWRTSQYSPFLYLGGNVLLSVST
                     VGVVAALAHPASWLGPALGWAPLRWLGVRSYGIYLWHFPIIVLTTPVVQRSVNLPLQI
                     LQVGATIAVAALS"
     gene            1317..1874
                     /locus_tag="VNT12_08625"
     CDS             1317..1874
                     /locus_tag="VNT12_08625"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWI46221.1"
                     /translation="MRLDDRARKLEGERIELRRHARENYHLYAEWYGDPEIWHLTSWA
                     PSPLSRSAVERLFEDRELSNTDDSFAIHIRGDDEPIGVISLMNKSEANESAELSVIVG
                     HQDDRHRGYGTEAIDRLLRYAFEEGGLNRIGLSVFDFNEEAISAYEKLGFVVEGRFRQ
                     AIKRRSGFHDAIIMSILKSEWEPSF"
     gene            complement(1864..>2340)
                     /locus_tag="VNT12_08630"
     CDS             complement(1864..>2340)
                     /locus_tag="VNT12_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46222.1"
                     /translation="PAARPYLHPGRAAMVLVGEEEAGWVGELHPEVAERFELHGWPVA
                     ALELDPALCHPDPGPRFEPFVNIPTVARDLAVLVEERVPVGDMLRLVQDSPSPLLTEV
                     RVFDVYTGSPVPEGRKSVALGFTFQGEKTLTDEEVDAEVGRILSMLEVDFDARVRS"
BASE COUNT          488 a          757 c          735 g          360 t
ORIGIN      
        1 gacagggcgg ccacggcaat ggtcgccccc acctggagga tctgcagggg aaggtttacg
       61 ctcctctgca cgaccggggt cgtgagcacg atgatcggga agtgccacag gtatatgccg
      121 taggagcgca ccccgagcca gcgcagcggg gcccacccca gcgccgggcc cagccagctg
      181 gcgggatggg caagggcggc cacgaccccg accgtactca ccgacagcag cacgttcccg
      241 ccgaggtaga gaaacggcga gtactggctc gtacgccaga tcaggagcgc aatgactgcc
      301 agcccggcga cccccgcccc gttcagaagc aagcgacgcc ggacggcgag gtccgcgcga
      361 aggttgcggc tcggccacac catggccagg gcggcgccta tgaggaggcc agaggcgcgg
      421 gtgtcggttc cttcgtagac tcgcgtcggg tcgacgcctg gctcgtagat cagggccata
      481 gcggcggcgg agagggctgc gccagccagc gtcaggccgg ccagggtgta gcgccaaggg
      541 acgtaacgca gcccgagcca gagcaaccag ggccagatca gatagaactg ctcctccaca
      601 gccagggacc acaggtgatc tagtggcggc ggtggcccga agcgggcgaa gtaggatgcc
      661 tcccggagga tgttccacca gttgctcacg tagccgacgg cggccagcac gtctcccctg
      721 aggcccggga gaagagaccg atccagcaat gtaacccacg ccaccaccac cgcaagcatc
      781 aggaaaaggg cgggcaggag ccgccgcgcc ctgcggagcc agaagtcacc tagccggagg
      841 ctccctacag ccgctcgttg gccgaggagc aaatccgtta tcaggtagcc gctcagggta
      901 aagaacacac caacgccgag cagcccccca ggtgcccagg ccggattcag atggtacgcg
      961 atcacggcaa gcaccgcaat ggcccgcagc ccgtcgagcc ctggcatgta gcgctgggaa
     1021 ctccctaccg gcttaggcat gatgccctcc ccaggcacca ataaggcacg gacgaatact
     1081 caccttgacc accccgagcg tcctatcagg gccgcagggt ataacgagtc gacgatccga
     1141 tgccttattg aggagtacgc tacggctccg gcccctccac tctactacaa cggctccgca
     1201 tggcccccca ttgtacccac gatctccgcg aggatggcag tgtgccctga gaaacgtgtt
     1261 ctggagcaca aaatgtagcg tggcacatcc ccgccaaacc cggtatattc ctggccgtgc
     1321 ggcttgacga cagagccaga aagctggaag gcgagaggat cgagcttcgg cgccacgccc
     1381 gcgagaatta ccacctctac gcggagtggt acggggaccc ggagatctgg cacctgacga
     1441 gttgggcgcc atcgcccctg agccgttcgg cggtcgagag gttgttcgag gacagggagc
     1501 tctctaatac ggacgactct ttcgccatcc acatcagggg cgatgatgag cctataggtg
     1561 tcatcagtct catgaacaag agcgaggcca acgagtcggc ggagctctcc gtgatcgttg
     1621 gtcatcagga cgaccgccac cggggttatg ggacagaggc catcgaccgg ctgttacgct
     1681 acgctttcga ggagggtgga ctcaaccgca tcggtcttag cgtcttcgat ttcaacgagg
     1741 aggccatctc ggcttacgag aagcttggct tcgtggtaga gggacgcttc cgccaggcca
     1801 taaagcgccg ctccggcttt cacgacgcca ttatcatgag catcctgaag tcggagtggg
     1861 aacctagctt ctgacgcgtg catcgaaatc gacttcgagc atgctgagga tacgcccgac
     1921 ttcagcgtcc acctcttcgt ccgtgagggt cttctcaccc tggaacgtga aacctaaggc
     1981 cacactcttt cgcccttctg ggacggggct tcccgtatag acgtcgaaga cgcgcacctc
     2041 ggtcagcaag gggctcggcg agtcctgaac caggcgcaac atgtcgccta ccggtacccg
     2101 ctcttcgacg aggacggcca ggtccctggc gacggtcgga atattgacga aaggttcgaa
     2161 gcgcggccca ggatccggat ggcacagggc gggatccaac tcaagagcag cgacaggcca
     2221 gccgtgcaac tcgaacctct ctgctacctc gggatgtagt tccccgaccc aaccagcctc
     2281 ttcctcgccg accagcacca tcgccgcgcg tccgggatgc agatacggtc tcgccgcggg
//
LOCUS       DATMJA010000360         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_108507, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000360 DATMJA010000000
VERSION     DATMJA010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108507"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..989
                     /gene="gluQRS"
                     /locus_tag="VNT12_08635"
     CDS             96..989
                     /gene="gluQRS"
                     /locus_tag="VNT12_08635"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWI46223.1"
                     /translation="MSVGRFAPSPTGPLHLGNLRTALLAWLFARSAGARFLVRVEDLD
                     RSRVRPGVEEIQLSDLRALGLDWDGPVVRQSERMELYEEAIARLDASGLLYPCYCTRA
                     EIRAAASAPHGIPASDRYPGNCRELTAPQRAVREASGRPPALRVRSDDVRVAFEDRLL
                     GRCEEEVDDFVVRRNDGTPAYQLAVVVDDAAQGIGEVVRGADLADSTPRQILLARLLG
                     LPEPRYAHVPLVIGPDGRRLAKRHGAVTLSDRDEGPAEVRAWMARTLGLARVGERPSP
                     DDLIARFDPERLPREPTVWSP"
     gene            1157..>2230
                     /locus_tag="VNT12_08640"
     CDS             1157..>2230
                     /locus_tag="VNT12_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWI46224.1"
                     /translation="MSQRFEGLQAEVEEKFGEQIVALRRDIHREPELNFDTERTAQKV
                     LAALDGLPLDIETGVAWNGIVATLRGGEGPTVALRADMDALPMEEDTGLPFSSEFEGR
                     MHACGHDGHTSMLVGAAHALAEMRDRLDGTVKFVFQPAEEGGGGGRVMVDEGVIDDVS
                     SIFALHLWPGLPFGQVATKAGPIMAAADGFEMEITGSGGHGAMPHLAADAVVIAAQVV
                     TALQILVSREVDPVEPAVLTVGKIGAGTAFNIIPEKARLGGTVRTLNADLRKRMPGRI
                     EVLARGVAKGMGGDANLDYTFSYPVTANDEGAAGRALSVAEELFGGQSVLELPNPSMG
                     GEDFAYFLEKVPGAFIWLGVGEDI"
BASE COUNT          400 a          693 c          757 g          380 t
ORIGIN      
        1 ctacctaccg aatcaccggt ccgagtctgg attccgtagg cttctcccac ggagcgctcc
       61 caccccgata gcagcgaggg tacagcgaga aggacatctc cgtcggacgc ttcgcgccga
      121 gccccactgg cccgctccac cttgggaacc tgcggacagc tctcctggcg tggctgttcg
      181 cgcgttcggc cggggcgcga tttctggtac gtgtggagga cctcgaccgc tcgcgggttc
      241 gtcctggcgt ggaagagata cagctctccg acctcagggc cctcgggctc gactgggacg
      301 gtccagtggt gcgccagtcg gagcggatgg agctgtacga ggaggccata gcccgcctcg
      361 atgctagcgg actgctctac ccttgctact gtacgcgcgc ggagatccgg gccgccgcat
      421 ccgcgccgca cggcatcccg gcttcagacc gttatcctgg caactgccgt gaactaacag
      481 cgccacagcg tgccgtgcgc gaggcctcag gacgccctcc ggcgctgcgg gtacgttcgg
      541 atgacgtccg agtagccttc gaggacaggc tcctcggtcg ctgtgaggaa gaggtggacg
      601 acttcgtcgt gcgccgcaac gacggcactc cggcctacca gctcgccgtt gtggtcgatg
      661 acgccgcgca ggggataggc gaggtggtga ggggtgcgga tcttgcggac tcgaccccgc
      721 gccagatcct gctggcacga ctgctcggct tgccagagcc ccgatacgcc cacgtcccgc
      781 tcgtgatcgg ccctgacgga cggcgtctcg ccaaacgcca cggcgccgtc accctatcgg
      841 atcgggacga gggaccggcc gaggtacgcg cctggatggc ccgcacgcta gggctcgcca
      901 gggttggaga gaggccgtca ccggacgacc tcatcgcccg cttcgatccg gaacgactgc
      961 cgcgcgagcc gaccgtctgg tcaccataga gcgcggtctg accgcgctct atggtaactt
     1021 tcccttgcag ccccacggcg gtgtcagccc ttcagcaaac aaaaagctga ctagctgaaa
     1081 tactgactag ctgaccagcg cggattgggg aagggagacc cttcctcaat ccgccaagag
     1141 aggagacttt gtggacgtgt cccagagatt cgaagggttg caggcagagg tcgaggagaa
     1201 gtttggtgag cagatcgtcg ccctccgccg cgacatccac cgcgagcccg aactaaactt
     1261 cgatacggag aggaccgcac aaaaggtgct cgcggcactc gacggtctac cactcgacat
     1321 agagacgggc gtcgcgtgga acgggatcgt cgccacgctc cggggaggag aaggacctac
     1381 ggtagcgttg cgggcagaca tggacgccct ccccatggag gaggatacgg gcctgccttt
     1441 ctcctcggag tttgaaggca ggatgcacgc ctgtggacac gacgggcaca ccagcatgct
     1501 cgtcggcgcg gcccacgccc ttgcggagat gagggatcgg ctcgacggca ccgtcaagtt
     1561 cgtgttccag cccgcggagg aaggtggcgg aggcgggaga gtcatggtcg atgagggggt
     1621 gatagacgat gtctcctcca tcttcgcgct gcatctgtgg ccagggttgc ctttcgggca
     1681 ggtggcgacg aaggctggtc cgatcatggc cgctgccgac ggtttcgaga tggagatcac
     1741 aggctcagga gggcacggcg cgatgcccca cttggccgcc gacgccgtag tcattgcggc
     1801 acaggtggtt accgcgttgc agatcctggt ctcgcgggag gtagacccgg tcgagcctgc
     1861 cgtccttacc gtggggaaga tcggggcagg aacggccttc aacatcatcc cggagaaggc
     1921 acgcctggga gggacggtca ggaccctgaa cgctgacctc cgcaagagga tgccaggacg
     1981 tatagaagta ttggcccgcg gcgtcgcgaa ggggatgggc ggcgacgcaa atctggatta
     2041 cactttttct taccccgtca ccgccaacga cgaaggtgct gcgggccgtg ccctgagcgt
     2101 ggcagaggaa ctcttcggcg ggcagtccgt tctagagttg cccaacccgt cgatgggcgg
     2161 cgaggacttc gcgtacttcc tggagaaggt gcctggggcc tttatctggc tcggcgtcgg
     2221 ggaagatatc
//
LOCUS       DATMJA010000361         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_168090, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000361 DATMJA010000000
VERSION     DATMJA010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168090"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VNT12_08645"
     CDS             complement(<1..724)
                     /locus_tag="VNT12_08645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566095.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWI46225.1"
                     /translation="MPFNLIRDKGREGRRGYTLPKPDVEAKEPESILPESVLRKEKPR
                     LAEVDEPTVIRHYTNLARENEGIDTTFYPLGSCTMKHNPRANEAAAVVPGFAGLHPLQ
                     PEAQAQGALRVMHQLQGFLAEVSGLPYVSLQPLAGAQGELTSILMVKKFFEDAGDDGR
                     TAVLVPSTAHGTNPATASMAGFGAKEVGLDEHGCVDVDELREMVDESTAALMITNPNT
                     CGVYERNIAEIAAVLHEAGAFLY"
     gene            complement(714..2003)
                     /gene="gcvPA"
                     /locus_tag="VNT12_08650"
     CDS             complement(714..2003)
                     /gene="gcvPA"
                     /locus_tag="VNT12_08650"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWI46226.1"
                     /translation="MARFTPHTEADVQEMLDAIGVETLDDLFADVQPRYEGELDLPPA
                     LSEYEALREVDALAKENAWGLPIFLGAGAYDRIVPSAIGAIISRGEFLTSYTPYQPEI
                     SQGTLQSIFEFQSMISELTGLEISNASVYDGANAVVEAALMTARLAKRDPKVAVSAGL
                     NPRYREVIETYRVEVVDLPFEDGATDFSNLPDDVSGVFVQSPNFFGVVEDIGAASEAA
                     HDVDALCVAVCDPISLSVLEAPGNLGADVAVGEAQPLGMPLMFGGPYAGYMATREDFV
                     RQLPGRITGETIDKDGKLAYVLTLRGREQDIRRARANSNICTNQALTALAATVYTALM
                     GPEGLREVAKLSISKAHYLAERLQASGFGLRYPDAPFLWEFAVELPDVPRANEALLEA
                     GIVGGLDLGDGAMLVAVTEKRTREELGAFVEVVADAL"
     regulatory      complement(2110..2225)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(2282..>2576)
                     /locus_tag="VNT12_08655"
     CDS             complement(2282..>2576)
                     /locus_tag="VNT12_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWI46227.1"
                     /translation="IADGFVPGIFQRHERLVDTALTVDSEEAVEEMRRLARKYGLFVG
                     PSSGANLIATRKVRESYPDLQNIVTLFCDEGEKYISDHFAERDSVTVGREAYT"
BASE COUNT          420 a          831 c          804 g          521 t
ORIGIN      
        1 tgtagaggaa ggcgcccgcc tcgtgcagga cggcggcgat ctcggcgatg ttgcgctcgt
       61 agacgccgca ggtgttgggg ttggtgatca tgagcgcggc cgtcgactcg tccaccatct
      121 cccttagttc atccacatct acgcacccat gctcgtcgag cccgacttcc tttgccccga
      181 acccagccat cgaggctgtc gccggattgg tcccatgcgc cgtcgaaggc acgaggactg
      241 cggtgcgccc gtcatcccct gcgtcctcga agaacttctt gaccatcagg atgctggtca
      301 gctctccctg cgccccggcg agaggctgca aagaaacgta aggcagcccg gagacctcgg
      361 cgaggaagcc ctgtagctga tgcatgactc gtagcgctcc ctgggcctgg gcttcgggct
      421 ggagcggatg gagtcccgcg aagccaggga ccacagcggc tgcttcgttg gcgcgtgggt
      481 tgtgcttcat cgtgcacgag cctaaagggt agaacgtcgt gtcgataccc tcgttctcgc
      541 gcgcgaggtt ggtgtagtgc cggatcacgg tcggttcgtc cacctctgct aggcgcggct
      601 tctctttacg gagcacggac tcgggcagga tgctctctgg ctccttcgcc tccacgtcgg
      661 gtttagggag cgtgtagcct ctgcggcctt cccggccttt gtcccggatc aggttaaagg
      721 gcatctgcta ccacctccac gaaggcgccg agttcttctc tggtgcgctt ctccgtcaca
      781 gccacgagca tcgccccgtc cccgaggtcg agcccgccta cgatcccggc ctccagcaat
      841 gcctcgttgg cccgtgggac atccggcaac tccacggcga actcccaaag gaaaggcgca
      901 tccggatatc gaagcccaaa tcccgaagct tgaagcctct ctgccagata atgggccttc
      961 gagatggaga gtttggccac ttcgcgcagt ccctctggtc ccatcagggc cgtgtaaacg
     1021 gtcgcggcca gggccgtgag cgcctggttg gtacagatgt tggagttcgc cctggcgcgc
     1081 ctgatgtcct gctcgcggcc ccgcagggtg aggacgtagg ccagctttcc gtctttgtct
     1141 atggtctctc ccgtgatacg tcccggaagc tggcggacga aatcctccct ggtcgccatg
     1201 tagcctgcgt acgggccacc gaacatgagc ggcatcccga gcggctgggc ctcgccaacg
     1261 gccacgtctg ccccgaggtt ccctggggct tcgagtacgg agagggagat cgggtcacac
     1321 accgcaacgc agagcgcgtc cacatcgtgc gccgcctccg aggcggctcc tatgtcctcg
     1381 acgaccccga agaagttcgg actctgcacg aagactcccg agacgtcgtc agggaggttg
     1441 gagaagtccg tggcgccgtc ttcgaatggg aggtcaacga cttcgacgcg gtacgtctct
     1501 atgacctcgc ggtagcgggg gttgaggccg gcggagactg cgactttggg gtcacgcttc
     1561 gccagcctcg ccgtcatgag ggcggcttcc acgacggcgt tcgcgccgtc gtatacggat
     1621 gcgttggaga tttcgagccc tgtcagctcg gagatcatgg attggaactc gaagatcgac
     1681 tgcagggtac cctggctgat ctccggctgg tacggagtgt acgaggtcag gaactcgccg
     1741 cgcgagatga tcgcgcctat ggcggagggt acgatcctat cgtaggcccc cgcaccgagg
     1801 aagatcggga gcccccaggc gttctccttg gccagggcgt ctacctctct caacgcctcg
     1861 tactccgaga gggccggcgg aaggtcgagc tctccttcgt acctcggctg aacgtcggcg
     1921 aagaggtcgt ctaaggtctc gaccccgatg gcgtccagca tctcctgtac atcggcctct
     1981 gtgtgcggtg tgaatcgcgc cacgcgccta taccccctgt ctcgggtgag cgttgctctt
     2041 tccgccctgt cccaagcctg agagtgttac cccgtcggcg ccggtccgtg ccggactctc
     2101 caggacgccc tccggctcgc ggtccttctg cctgagagat tcgggccgct atcgcaaccc
     2161 tttcaccttc ggcggccgtc acgaggtttc tccaccctca tacgactctc tcccgcggcc
     2221 ctcatcgggc aagttcggac tatagcataa gggacgtggc accggatgtg tcaacgtctc
     2281 tctacgtgta cgcttcgcgc cctaccgtga ccgagtcccg ctccgcgaaa tgatcgctta
     2341 tgtacttctc gccctcgtcg cagaagaggg tcacgatgtt ctgaaggtcg gggtaggact
     2401 cgcggacctt tcttgtggcg atgaggttgg cgccgctcga agggccgacg aagaggccat
     2461 atttcctggc gaggcggcgc atctcctcta cggcctcttc ggagtcaact gtcagggcgg
     2521 tatcgaccag tctctcgtgg cgctggaaaa tgccgggcac aaagccgtca gcgatg
//
LOCUS       DATMJA010000362         4190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_819728, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000362 DATMJA010000000
VERSION     DATMJA010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819728"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..372
                     /locus_tag="VNT12_08660"
     CDS             115..372
                     /locus_tag="VNT12_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46228.1"
                     /translation="MEREPLSSEADALWRRLWEIWQDNDEEDVVLDSTELAELEEEIP
                     GLEKRMKTALAYLQRARYIQYRSGVGEDGIEPILYDVYEPR"
     gene            complement(420..1064)
                     /locus_tag="VNT12_08665"
     CDS             complement(420..1064)
                     /locus_tag="VNT12_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI46229.1"
                     /translation="MNENSGKHAAVNGLDLYYEIHGEGEPLILLHGGVGAIEMFGEVL
                     PLLAEGRRVIAVDLQAHGRTVDIDRPMTFEAMADDVAALIEHLGLGEADVMGYSLGGG
                     VALRTAIQHPEVVRKLVLVSTPFKRDGWYPEVLAGMEQMGPEAAEPMKQTPMYQLYSS
                     VAPRPEDSGGREQDYGPNEKGERGRQYRAADDLAQLAVDGELLGRGHPCGERHG"
     gene            complement(1061..1621)
                     /locus_tag="VNT12_08670"
     CDS             complement(1061..1621)
                     /locus_tag="VNT12_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI46230.1"
                     /translation="MTRQLLFVHGGGEGAYEEDRKLAASLRDALGGGYEVQCPKMPDE
                     DSPQYAAWRDRIASELAELDGEIFLVGHSLGASILLKYLSEERTATSISGTFLVAAPY
                     WGVEDWEVDEYVLREDFTSKLPEGLPIFFYHSRDDEWVPFAHLGLYAEKVPHARFREF
                     YGRGHQFDDDLSEVAHDIEGVERSES"
     gene            complement(1864..2484)
                     /locus_tag="VNT12_08675"
     CDS             complement(1864..2484)
                     /locus_tag="VNT12_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWI46231.1"
                     /translation="MGKVTSGLTMSLDGFIAGPNDGPEHPLGEGGMRLFDWYSSGDTD
                     YVVPSGGMTFKVSSQAAEMLREVFSSIGAIVTGRRTFDITNGWGGRHPIGEHVPIFVL
                     THSVPEGWDYEGSPFTFVTDGVESAVEQARAAAGEKNVAVGAASLVQQCLRAGLLDEV
                     HVDVVPVLLGDGVRLFDHLGTEHIELERTGLIADPDVTHMTFRVVR"
     gene            complement(2661..3773)
                     /locus_tag="VNT12_08680"
     CDS             complement(2661..3773)
                     /locus_tag="VNT12_08680"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWI46232.1"
                     /translation="MTEIQNQVQEVIDELVESGAERGLQVAVYRHGEQVADAVAGVAD
                     PESGRPVESDTPFYCYSVGKGATSTVAHVLVERDLFGYDTPVVDLWPEFGAHGKETAT
                     VRHVLTHTVGVPGIPADTTPEDLCDWDKMCSAIAEAEPWWEPGTKVAYHAYPFGYIVG
                     EIVRRTTGKPISQVLREEVAVPLSIADEIYFGVPESELGRLARLEDMEGSAEMLASMP
                     DDSPIFKLGPRQVTPTAEFGNRPDILMADIPAGGKMSARAVARMYAALVDEVDGVRLV
                     SPERLREISAGAFSGVDEIMGFPTTWALGYSVGRLGADPQETKSVLGIGGVGGSFACA
                     DTATGVTWALTKNRLTAGFEAAEQISEIVTKAVGED"
     gene            complement(3800..>4190)
                     /locus_tag="VNT12_08685"
     CDS             complement(3800..>4190)
                     /locus_tag="VNT12_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI46233.1"
                     /translation="HGHTGLGRNPDVLHLVRPRVFPGVYVGGFGIACTAIEALTKQLA
                     AEVGPDGIRVNCLRSEGIPESWEVASTGEWSAPPEEIGAFLEQRSLLGRVTTLADVGN
                     AATFLASDRAAATTGTVFNLTSGTVVD"
BASE COUNT          780 a         1430 c         1175 g          805 t
ORIGIN      
        1 cgagcctgcc agggtcgcgc aacgcagtca ggctcgcggt agaaaaactc ctcgttccag
       61 agctggccca catcgtgttc gagcttcgca agcatgagga aagcgggtag agagatggaa
      121 agagaacccc tatcgtccga agcagatgcg ctctggcgca ggctgtggga gatctggcag
      181 gacaacgacg aggaggacgt agtcctcgat tccaccgagc tggccgagct cgaagaggag
      241 atccccggcc tcgaaaaacg gatgaagacg gccctcgcct acctccagcg cgcccgctat
      301 atccagtacc gctccggggt cggcgaggac ggcatagagc ctatcctcta cgacgtctac
      361 gagccgcgct gagcccgtga aaacctagtg gagtgaggag tatgacctgg ctactcttgc
      421 tacccgtggc gctcgccgca gggatggccg cgcccaagca gttcgccatc aacagccagc
      481 tgcgccaggt cgtcggcggc cctgtactgg cgtccgcgct ctcctttctc gttgggaccg
      541 taatcctgtt cgcgaccacc gctgtcttcg ggcctcggcg cgacgctgga gtagagctgg
      601 tacatcggcg tctgtttcat cggctctgcg gcctccgggc ccatctgttc catccctgca
      661 aggacctcgg ggtaccatcc atcgcgcttg aacggggtcg agacgagcac tagcttcctt
      721 accacctcgg gatgttggat cgccgttcgc agggcgaccc cgccgccgag agagtagccc
      781 atgacgtcgg cctctcccaa ccccaggtgc tcgatcaggg ctgccacgtc gtccgccatc
      841 gcttcgaaag tcatcggacg gtcgatatcg actgtgcgac catgggcctg cagatccacg
      901 gcgatgaccc tgcgaccttc tgccagcagg ggcaacacct ctccgaacat ttcgatagcg
      961 ccgacaccgc cgtgcagcag gatcaggggc tcgccttcgc cgtgtatctc atagtaaagg
     1021 tcgagaccgt tcaccgcggc gtgtttaccc gagttctcgt tcatgattcg ctcctctcca
     1081 caccctcgat atcatgggcg acctctgaga gatcgtcatc gaactgatgc ccgcgtccat
     1141 agaactcgcg gaatcttgca tgcggaacct tttccgcata gagcccgagg tgtgcgaacg
     1201 gcacccactc gtcgtcgcgg ctgtggtaga agaaaatcgg caggccctcg gggagcttcg
     1261 aggtgaagtc ttctcgcaga acatactcat caacctccca gtcctcgaca ccccagtagg
     1321 gtgccgcgac caggaacgtt ccggatatgg atgtggccgt cctctcctcg gagaggtact
     1381 tcagcaggat cgaagccccc agagaatggc cgacgaggaa gatctcgcca tcaagctcag
     1441 cgagttcact tgctatccgg tctctccacg ccgcgtactg cgggctatcc tcatctggca
     1501 tcttcggaca ttgcacctcg tagccgccgc ccagcgcgtc tcgcaagctc gccgccagtt
     1561 tcctgtcttc ctcgtacgct ccttctccgc caccgtgaac aaacagcagt tgtctagtca
     1621 ttcacgtgtt cctgactggt ctactttctt cccatcggcg aacgatagac cgttcgcaac
     1681 cagggcatgc cggagttgcg caagcgcctt tggccccacg ccatgtagtc tctcaacctc
     1741 cgcttcgctc accctggtaa gctgctcgag ccagcggtag ccagcgttga tgagcgcccg
     1801 ctgcgcaggc ttggacaatc cgggtggaag gtcggtttcc tgctgaggat tcatggtaac
     1861 cctctacctg acgacccgga aagtcatgtg ggtcacgtct gggtctgcta tcagcccggt
     1921 cctctccagc tcgatatgtt cggtccccag gtgatcgaac aacctcacgc cgtcccccaa
     1981 cagcacgggg acaacgtcta cgtgtacttc gtccagtagc cctgccctga gacactgctg
     2041 gacgaggctc gcagcgccca ccgccacgtt cttctcgcca gccgccgccc tggcctgttc
     2101 gaccgcgctc tccacgccgt cggtgacgaa ggtgaacggc gacccctcgt aatcccatcc
     2161 ttcggggacg gagtgggtca agacgaagat cggaacgtgt tcgcctattg gatgtctgcc
     2221 gccccaaccg ttcgtgatgt cgaaagtcct ccgtcccgtc acgattgctc caatcgagga
     2281 gaacacctcg cgcagcatct ctgcggcctg ggacgagacc ttgaacgtca tcccgccgct
     2341 cggcacgacg taatcggtgt cgccgctgga gtaccagtcg aagagccgca ttcctccttc
     2401 accgagcgga tgctccggcc cgtcgttcgg cccggcgatg aacccgtcca gcgacatcgt
     2461 gagtcccgaa gttactttgc ccatgtctac tctccttcct atatctagtt acgggcgggt
     2521 atctgctgca cacaccagcg gtttcccacc gggaccgtca cgacctctat cttccaaacc
     2581 atagtgcttc ttcctttcgc cagactagac gctgattccc cgacctcaac gtcctctcca
     2641 gtagagggct cgagttcgcc ctagtcctct ccgactgcct tggtaacgat ctcggagatc
     2701 tgctcggcgg cctcgaaacc ggcggtgagc ctgttcttcg tcagggccca tgtgacgcct
     2761 gtcgcggtgt ccgcgcatgc gaagctcccg ccgacgcccc cgattcctag cacgctcttc
     2821 gtctcttgcg ggtccgctcc caggcgaccg acagagtaac cgagcgccca ggttgtcggg
     2881 aagcccatga tctcatctac accgctgaac gccccggcgg atatctcgcg cagccgctcg
     2941 ggcgagacga gccggacgcc gtccacctcg tccaccaagg cggcgtacat tcgcgctacg
     3001 gcccgcgctg acatcttgcc gccggccggt atgtcggcca tcaagatgtc gggacggttg
     3061 ccgaactcgg cggtcggggt cacctgccgg ggccccagct tgaagatagg agaatcatcg
     3121 ggcatcgagg caagcatttc cgcactacct tccatgtctt ccagcctggc cagcctgccc
     3181 aactcagact cggggacgcc gaagtagatc tcatcggcga tactgagcgg aacggccacc
     3241 tcctcacgaa gcacctgcga gatgggcttg cccgtagtac ggcgcacgat ctccccaacg
     3301 atgtagccga acgggtatgc atgataggca accttcgtac cgggctccca ccagggctcg
     3361 gcttcggcga tcgcggagca catcttgtcc cagtcacaca ggtcctctgg cgtcgtatcg
     3421 gccggtatgc caggcacccc gaccgtatgc gtcagcacgt gccgcacggt agcagtctct
     3481 ttaccgtggg cgccgaactc gggccaaagg tccacgaccg gtgtatcgta gccgaacaaa
     3541 tcacgctcga cgagcacatg cgccaccgtc gaggtcgcgc ccttgccgac cgagtagcaa
     3601 tagaaaggcg tgtcagactc gacgggacgc ccggactccg ggtcggcgac accagccact
     3661 gcgtcggcta cctgctcgcc gtgccggtag acagcgacct gcagaccccg ctcggctcca
     3721 gactcaacca gctcgtcgat gacttcctgt acctgattct ggatttcggt catgatccca
     3781 cccctttcca aggctacgtc tagtctacaa ccgtgccgct ggtcagattg aaaaccgttc
     3841 ctgttgtcgc ggcggcccga tccgaggcca gaaacgtcgc agcgttgccc acgtcggcca
     3901 gggtcgtcac ccgcccaagc aaagatctct gttcgagaaa cgccccgatc tcctcgggtg
     3961 gagccgacca ctctccggtc gaagcgacct cccaggactc cgggatcccc tccgaacgca
     4021 ggcagttcac gcggatgccg tccggcccga cctcagccgc aagctgcttt gtgagagctt
     4081 cgatcgccgt gcacgcgatc ccgaagcccc ctacatagac accaggaaaa acgcgcggac
     4141 ggacgaggtg gagaacgtca ggattacgcc cgagccctgt gtggccatgc
//
LOCUS       DATMJA010000363         3875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_641798, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000363 DATMJA010000000
VERSION     DATMJA010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641798"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..1424)
                     /gene="rodA"
                     /locus_tag="VNT12_08690"
     CDS             complement(324..1424)
                     /gene="rodA"
                     /locus_tag="VNT12_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWI46234.1"
                     /translation="MAHRLLRSDVLLFLTAVALGGIGVFAIYAAEADYRQLYAVNQAL
                     GLVAGLVGAVVLALLDYRWLGRHLRFVYGATIVMLVVVLLAGFSVNGAQSWIGVGPVE
                     VQPSEFAKPLSIVVLAGYVAANSFAHTMTFLKALGIMSVPLLLVLAQPDLGTAMVFGA
                     LFVAVVFVGGARWYQLGGLFAAGCVTVYFAIKLRILEDYQIARLTSFLDPQSVPGVSY
                     QVENSKMAIGSGGLMGKGLGSSGTLGDLGYLPEDRTDFIFSNLAEKVGFVGSVVVLIL
                     FFLLVWRVLHAATISCDRFGVLIAVGVATMLTFHALINIGMAMGMMPVTGLPLPFVSY
                     GRSNLLVSMMSVGLVQSIVIQARLKSEGNPRT"
     gene            1676..1975
                     /gene="rplU"
                     /locus_tag="VNT12_08695"
     CDS             1676..1975
                     /gene="rplU"
                     /locus_tag="VNT12_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWI46235.1"
                     /translation="MFAVVKSGGKQYKVREGQELVVDRLEGNVGDTVELPVGFSVDEG
                     GFDLAEKRARVEILDHLRGEKIHVYKYKPKKDYRKKTGHRQELTRVKVLEVSDGS"
     gene            1965..2240
                     /gene="rpmA"
                     /locus_tag="VNT12_08700"
     CDS             1965..2240
                     /gene="rpmA"
                     /locus_tag="VNT12_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWI46236.1"
                     /translation="MAHKKGGGSSRNGRDSAGRRLGVKAYGGQVVSAGSIIVRQRGSK
                     VHPGQNVGKGSDDTLFARVSGRIDFDRSRGKSVVSVIEDPVLQEATA"
     gene            2272..3510
                     /gene="obgE"
                     /locus_tag="VNT12_08705"
     CDS             2272..3510
                     /gene="obgE"
                     /locus_tag="VNT12_08705"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWI46237.1"
                     /translation="MQFVDEASFIVRAGRGGDGSVSFNREKYKPRGGPDGGRGGDGGD
                     VVLISTEDLSTLEPHAYRNVVKAGRGGHGSSNNKAGERGKDAVLEVPVGTQVFDDDSF
                     LADLSKPGESFVVAGGGEGGRGNGSFATSTRQAPAFREKGLPGEERGISLELKVLSDV
                     GLVGLPNAGKSSLLRTLSAARPRVGEYPFTTLSPQLGVVDERTYRERFVVADIPGLIS
                     GASEGRGLGNRFLRHVARARLLALVLDASEDPEGAEGTLRAELHAARLSEKPTVVVLN
                     KVDLLDEELRAYLREVFSDAPQVSATTAEGVAALRDTFEQRLREMGEHAVGEMLEREH
                     RVFRPTWKGVRVEHENGGYVVSGEGVERLALKTDWDNREGVERFQHELQRIGVLGALR
                     RAGAEPGDEVRIGETVFDFQ"
     gene            3518..>3875
                     /locus_tag="VNT12_08710"
     CDS             3518..>3875
                     /locus_tag="VNT12_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWI46238.1"
                     /translation="MRIGIFGGTFDPVHQGHMIVAEQVMTELDLARVVFVPGGIPPHK
                     EASSVRATAPDRLAMVEAAVEENERFSVDRVEIDSGRPMHSVEIVSILGERSPDDELF
                     FITGADEVSNLLAWSDP"
BASE COUNT          779 a         1159 c         1286 g          651 t
ORIGIN      
        1 agaaagaatc gggccgctcg cgaacgatca cgggccaagc ttagcagctt ggcagctcgc
       61 tgcctctggc agcttgtcag cacgctcagg ctgacgcctt cgcttgtcag cccgccgcct
      121 cgctctgctc ggcggcttct cagcacttca gcaagtcgtc tcgtagcctc cccacgaacg
      181 tagaacccga ctgccgctgg tagcggttcg tcgtgcgcgg cagggcaagg ttcggaacgg
      241 gagaagctga aatgctgaca agcgagggcc ttataggccc gaagagtgct gacaaggcgc
      301 gtagcgccgt gctgaccagc tgctcaagtc cgagggttcc cctcggactt gagcctcgcc
      361 tggatcacga tactctgcac tagcccgacc gacatcatgc tgactagcaa attgctgcgc
      421 ccgtagctca cgaaggggag tggcagcccg gtaacgggca tcatgcccat ggccatcccg
      481 atatttatga gcgcgtggaa cgtcagcatc gtcgccaccc caaccgcgat gagcacgccg
      541 aagcgatcgc acgagatcgt cgcggcgtgc agcacgcgcc agaccagcaa gaagaaaagg
      601 attagcacaa ccacagaccc tacgaatccg accttctccg cgagattgga gaatatgaag
      661 tccgtgcggt cctccggcag atagccaagg tcaccgagag tccccgacga tccgagaccc
      721 ttgcccatga gcccccctga gcctatggcc atcttggagt tctcgacctg gtagctcacc
      781 ccagggacgc tctggggatc gaggaacgag gtgagacgtg ctatctggta gtcttccagg
      841 attctgagct tgatggcgaa gtagacggtc acgcacccgg ctgcgaagag ccccccaagc
      901 tggtaccacc tggctccacc tacgaagaca acggcaacaa agagggcccc gaacaccatc
      961 gcggtcccga ggtctggttg cgcgagcacg agcaaaagtg gcacggacat gatgccgagg
     1021 gctttcagga aagtcatggt gtgcgcaaaa ctgttcgcgg ctacatatcc ggcgagcacc
     1081 acgatcgaca gcggcttggc gaactcggaa ggctgcacct cgacagggcc gaccccgatc
     1141 cagctctgcg ccccgttgac cgagaagcct gcgaggagca cgaccaccag catcacgatc
     1201 gtcgccccgt agacgaacct gaggtgtcgc ccaagccacc tgtaatccag cagcgcgagc
     1261 acgacggcgc cgacgagacc ggccacaagc ccgagggcct ggttgacagc gtagagctgc
     1321 ctgtagtcag cctcggcagc atagatggcg aagaccccga tcccacccag cgctacagca
     1381 gtgaggaaaa ggagcacgtc ggagcggagt agccgatgcg ccatgcctac cttctcttgt
     1441 ttcttgcccc tggagacagg gaccgctgga ttggtcctta ctgtacgcat gcctcaaaga
     1501 tacacaatcc ccgcacctta ggcaacgccc gccagaagaa ccctcaacca acctgttgcc
     1561 cgcacggtaa gccggcctcg gcgtgtggtc gacgcggagc atggcggtta tactgttgcc
     1621 ttgtgggatg cgttttgtaa gcccgtcgca gcctgataaa ttctaggaga tcgagatgtt
     1681 tgcggttgta aagagcggtg gcaagcagta caaggtgaga gagggccagg aactggtcgt
     1741 cgaccgcctt gagggcaacg tgggggacac tgtagaactt cctgtcggct tctcggttga
     1801 tgaaggggga ttcgacctcg ccgagaagcg ggccagggtc gagatcctgg accatctgcg
     1861 gggagagaag atccacgtct acaagtacaa gccgaaaaag gactacagga agaagaccgg
     1921 gcacaggcag gagctgaccc gggtaaaggt gctggaggta tctgatggct cataagaagg
     1981 gcgggggctc ctcgcgtaac ggccgtgatt cggctggcag gaggctcgga gtaaaggcgt
     2041 atggaggcca ggtggtcagc gcagggagca taatcgtgcg ccagcgcggc tcgaaggtgc
     2101 acccgggaca gaacgtgggc aagggctcag atgacaccct ctttgccagg gtctcgggca
     2161 ggatcgactt cgaccgctct cggggcaaga gcgtcgttag cgtgatcgag gatcccgttc
     2221 tccaggaggc cacggcctga ccatcgtgcg ttacgggggt ctttagcccc tttgcagttc
     2281 gtagatgaag ccagcttcat cgtccgcgcg ggacgcgggg gcgacggcag cgtctcattc
     2341 aaccgcgaga agtacaagcc gcgcggcggc ccagacggcg gtcgcggcgg cgacggcggg
     2401 gacgtggtcc tgatctccac tgaagacctc tctactctcg agccgcacgc gtaccgcaac
     2461 gtcgttaaag cgggccgcgg cgggcatggt tcatccaaca acaaggctgg cgagcgcggc
     2521 aaggacgcgg tcctcgaggt ccccgtaggg acccaggtct tcgacgatga cagcttcctg
     2581 gccgacctct cgaaaccggg agagagcttc gtcgtcgccg gcggcggcga gggcggacgc
     2641 ggcaacggat ccttcgcgac ctccacccgt caggcgccgg cattcaggga gaagggtctg
     2701 ccgggcgagg agcgagggat cagcctcgag ctcaaggtcc tctcggacgt cggcctcgtc
     2761 ggtctgccca acgcggggaa gtcttcgctg ctccggacac tcagcgccgc tcgtccgagg
     2821 gtgggcgagt atcctttcac taccctctcg ccccagctcg gggtggtaga tgagagaacc
     2881 taccgcgaac ggttcgtggt ggcggatatc cctggcctta tctctggggc cagcgagggc
     2941 aggggcctcg gcaaccgttt tctcaggcat gtggcgcggg cgcgactcct tgcgctcgtg
     3001 ctcgacgcga gcgaagaccc ggaaggggcc gaaggcacgc tgcgggccga gctccacgca
     3061 gccaggctct cggaaaagcc cactgtcgtg gtgctgaaca aggtcgacct cctcgacgag
     3121 gagctcaggg cgtacctgcg ggaggtcttt tcggatgccc ctcaggtctc cgcgaccacg
     3181 gcggaaggtg ttgctgcgct gcgcgacacc ttcgagcaga ggctgcgcga gatgggcgag
     3241 cacgccgtcg gtgagatgct tgagcgtgag catcgcgttt tcaggccaac ctggaagggg
     3301 gtcagggtcg agcacgagaa cggtggctac gttgtctcgg gggagggcgt cgagcggctc
     3361 gcgctcaaga cagactggga caacagggag ggtgtcgagc gtttccagca cgagctgcag
     3421 aggatcgggg tactcggagc cttgcggcgg gcaggtgcag agccaggcga tgaggtcagg
     3481 atcggcgaga cggtattcga cttccagtga ggtgggcgtg aggattggga tcttcggcgg
     3541 gactttcgac cccgtgcatc aggggcatat gatcgtcgca gagcaggtca tgaccgagct
     3601 cgatctggcg cgcgtcgttt tcgtgcctgg cggcatcccg ccgcacaagg aagcctccag
     3661 cgtcagggct accgcgcccg accggctcgc catggtcgag gcggccgtcg aagagaacga
     3721 acgcttctcc gtggacagag tggagattga ctccgggcgt cccatgcaca gcgtggagat
     3781 cgtcagcatc cttggggagc gctcgcccga tgacgagttg ttctttatta cgggcgcgga
     3841 cgaagtgtcg aacctgctcg cctggagtga tccag
//
LOCUS       DATMJA010000364         5692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_760729, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000364 DATMJA010000000
VERSION     DATMJA010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760729"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VNT12_08715"
     CDS             complement(<1..759)
                     /locus_tag="VNT12_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI46239.1"
                     /translation="MEYRNLGRTGVKVSELCLGCMMFGGRTGEEDSADIIDLAIDGGV
                     NFLDTANVYSTGASEEAVGKALKRNGKRDAMVLATKVHGAMDKDDPNAAGNHRRHIIE
                     QCEASLRRLQVDYIDLYQIHRPSSDVPIDETLRALDDLIRAGKVRYVGTSTYAAWQLV
                     EALWAAQELGLNRFVSEQPPYHLLDRRIERELIPFAQTYGVALIPWSSLAGGFLTGKY
                     TRDESQSPGDSRYGLQPRRMGRNHFTDEAFGVLEA"
     gene            905..1897
                     /locus_tag="VNT12_08720"
     CDS             905..1897
                     /locus_tag="VNT12_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWI46240.1"
                     /translation="MSKVRWGILGTGAIARQFVQGLNSLPEAKVLAVGSRSKASAERF
                     AAKRGIPRRHASYDNLASDPDVDVVYVATPHPFHAENATLCLEAGKAVLCEKPFSVNA
                     AEAQRVVGLAREKGLFLMEGMWTRFFPLMEELRRLVSEGAIGELRMLNVDFGFRAGLD
                     PASRLFDPKLGGGALLDVGVYCVSLASMILGRPSGSVGLSHLGETGVDEQASIVLEHE
                     GGRLANLSIGIRTATPQEATIMGTEGYVRIHAPWWRPKSMTISRPGAEDETVDAPVSG
                     NGFNYEAAEVMRCLEAGKMESDIMPLDETISVMRTMDGIRAAWGLTYPGEEVAS"
     gene            1894..2868
                     /locus_tag="VNT12_08725"
     CDS             1894..2868
                     /locus_tag="VNT12_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI46241.1"
                     /translation="MNYARVPGVEKPVARIVQGTVMIRSEEEGQEERSFALLDEVFAQ
                     GGTTFDTAHQYGGGDCERTLGRWVKDRGLREEVVVIGKGAHHNEDRKRVTPFDITSDL
                     YDSLARLGFDYIDLYVLHRDDPSVPVGPIVEVLNEHKEEGRIHAFGGSNWRSERLREA
                     NDYARENGLTPMVASSPNLTLAVQVKEPWPDCVSISGDGGEKDRRWYGETRMPLFTWS
                     SLAGGFFSGRFTRDNLDTFDATLDRICVETYCYEENFGRLDRAGVLARAKGLTVPQVA
                     LIYVLAQPLEIFALVGCNVGEEFRANVEAGAVVLTPEEIAWLENGDTP"
     gene            2837..3034
                     /locus_tag="VNT12_08730"
     CDS             2837..3034
                     /locus_tag="VNT12_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46242.1"
                     /translation="MPGSRTATPLRGGEEIVRVVQAGLGIWGRSWSGLVAGAPGVELA
                     AAVDPDHVDVAALLAARGAKL"
     gene            3087..4088
                     /locus_tag="VNT12_08735"
     CDS             3087..4088
                     /locus_tag="VNT12_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWI46243.1"
                     /translation="MARPVTLFTGQWADLALEELARKCGEWGFDGLELACWGDHFDVD
                     KALSESGYIEGRWEILRSNGLNCWAISTHLVGQAVCDPIDARHGATLPPDVWGDGDPE
                     GVRRRAAEKVMDTARAAARFGVTQVNGFTGSSIWHLLYSFPPNDFEEIERGYQDFAER
                     WNPIIDVFDEEGVRWGLEVHPTEIAYDFVTTRNALDAIGNREGFGINFDPSHFAHQFL
                     DSAAFIEEFADRIYHFHIKDSRKRLNGRRSILGSHLDFGSPERGWDFVSPGHGDVNLE
                     GCIRALNRIGYEGPLSIEWEDSGMDREYGARDALAFARRTDFEPSTQAFDAAFARKE"
     gene            4091..5263
                     /locus_tag="VNT12_08740"
     CDS             4091..5263
                     /locus_tag="VNT12_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWI46244.1"
                     /translation="MERGEIGVGLVGYKFMGRAHSNAYRQVARFFDVDPTPRMAAICG
                     RDEAGVREAADTLGWDSYETDYRKLIARDDIDLVDVVTPGNTHREIVLTALEAGKHVL
                     CEKPLANNLSTAQEMFIAARNAGTVNMVCFNYRRAPAVQLAKKLVEEGRLGEIRHWRA
                     TYLQDFIMDPSFPLIWRLQKEVSGSGALGDIGAHIIDLAHFVVGPISEVTGMTETFVE
                     ERPLEETSAGGGLSASGGPEMGEVTVDDAAAFLARFENGAIGTFEATRFAAGRRNQNA
                     FEVNGSKGSVAFDLERMNELEVFFTEDEPDVQGFRTVNVTEPDHPYTGAWWPPGHILG
                     YEHTFVHTIKDLMDGIKVGKSPAPTFEDGYRCQAVLDAVERSAASGEWRRPEMEGI"
     gene            5266..>5692
                     /locus_tag="VNT12_08745"
     CDS             5266..>5692
                     /locus_tag="VNT12_08745"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWI46245.1"
                     /translation="MAEEPRVTIWNEYRHEKSDEQVAKIYPEGMHAVLAAALTEAGFE
                     VHAATLDEPEHGLSDEVLDATDVLLWWGHMAHDEVSDETVEKVHQRVLGGMGLIVLHS
                     GHFSKIFKKLMGTTCDLKWRHDDTERLWVVAAGHPITEGL"
BASE COUNT         1096 a         1743 c         1892 g          961 t
ORIGIN      
        1 ggcttcgagt acgccgaagg cctcgtctgt aaagtggttg cggcccatgc gcctgggttg
       61 caggccgtag cggctgtcgc cgggggactg gctctcgtcg cgagtgtact ttccggtaag
      121 gaagccgccg gcgagggagg accaggggat cagggccacc ccgtaagtct gggcgaaggg
      181 gatgagctcg cgctcgatac gccggtcgag gaggtggtag ggaggctgct cgctcacaaa
      241 gcgattcagc ccgagctcct gtgcggccca taacgcctcg acgagttgcc aggcggcgta
      301 ggtagacgtg cctacgtagc gcaccttgcc ggcacggatc aggtcgtcga gagcccgcaa
      361 cgtctcgtcg attgggacgt cagagctcgg gcggtggatc tggtagaggt cgatgtagtc
      421 cacctgaaga cgcctcagcg aggcctcgca ctgctcgata atgtgccgcc tgtggttgcc
      481 cgcggcgttg ggatcatcct tgtccatcgc cccgtgcacc ttggtggcga gcaccatggc
      541 gtcgcgcttc ccgttccgtt tcagcgcctt gccgacggcc tcctcgctgg cgcccgtgct
      601 gtacacgttc gccgtgtcga ggaagttgac gcccccgtcg atggcgagat ctatgatgtc
      661 tgcagaatcc tcttctcccg tgcggccgcc gaacatcatg caccccaggc agagctcgct
      721 gaccttaaca ccggttcttc cgaggtttct atactccacg cttcgctcct tcgaggtaga
      781 cgtataccca ttcgatgcac atcgtaacgt tcgcgtaacc tccccggttt gattgtgcgc
      841 accttagtat gtagcatcgc ggcttgtgta tctggccgtg tgagggggaa ggcgcgcaga
      901 gatcgtgagc aaggtccggt gggggatact cgggacgggc gccatcgcga ggcagttcgt
      961 gcagggcctg aactccttgc ccgaggccaa ggtgctcgcc gtcgggtccc gctccaaagc
     1021 ttcggccgaa aggttcgccg ctaagagggg catcccgcgt cgccacgcaa gctacgacaa
     1081 cctcgcctca gaccccgacg tcgacgtcgt ctacgtcgct acgccccacc ccttccacgc
     1141 ggagaacgcc acgctgtgcc tcgaggccgg caaggccgta ctgtgcgaga agcccttttc
     1201 cgtgaacgcc gcggaagccc aacgggtggt cgggctcgcc agggagaagg ggctcttcct
     1261 catggagggt atgtggacca ggttcttccc gctgatggaa gagttgcggc ggctggtctc
     1321 ggaaggagcc ataggcgagc tgcgtatgct gaacgtggac ttcggcttcc gggcggggct
     1381 cgaccctgct tcccggctct tcgacccgaa gctcggcggg ggcgccctgc tcgacgtcgg
     1441 ggtctactgt gtctctttag cctcgatgat cctcggccgg ccttcggggt ctgtcgggct
     1501 ctctcacctc ggggagacag gcgtcgacga gcaggcctcc atcgtcctcg agcacgaagg
     1561 cggaagactg gccaacctgt ccatcggcat caggaccgcg acgccccagg aggcgacgat
     1621 catgggtacc gaagggtacg ttaggatcca cgccccctgg tggcgtccga agtccatgac
     1681 aatctccaga ccaggcgcag aggacgagac cgtcgacgcc ccggtctccg gcaacggctt
     1741 caactacgag gccgccgagg tcatgcggtg cctagaggct ggcaagatgg agagcgacat
     1801 catgccgctg gacgagacga tctccgttat gaggacgatg gacggcataa gagccgcgtg
     1861 ggggctcacg taccccggcg aggaggttgc gtcttgaact acgcgcgggt gcctggcgtg
     1921 gagaagccgg tagcgcggat cgtgcagggc accgtaatga tcagatccga agaggaagga
     1981 caggaagagc ggagcttcgc gctgctggat gaagtcttcg cgcagggcgg taccacgttc
     2041 gatacggccc atcagtacgg cggaggcgac tgcgagcgaa ccctcggccg gtgggtaaag
     2101 gaccgcggcc tgcgcgagga ggtcgtggta ataggcaaag gcgcccacca caacgaggac
     2161 cgcaagcggg taaccccctt cgatatcacc tccgacctct acgactccct ggctcgcctg
     2221 ggcttcgact acatagacct ctacgtcctc caccgcgatg acccttccgt gcctgtcggc
     2281 cccatcgtcg aggtcctaaa cgagcacaag gaggaaggca ggatccatgc cttcggcggc
     2341 tcgaactgga gatccgagcg cctccgcgaa gcgaacgact acgccaggga gaacggcctt
     2401 acgcccatgg ttgcgagcag tcccaacctc accctcgccg tgcaggtcaa ggagccctgg
     2461 ccagactgcg tgagcatcag cggggacggg ggagagaagg accgccgttg gtatggggag
     2521 accaggatgc cgctgtttac ctggtcgagc ctcgcaggtg gtttcttctc cggccgcttt
     2581 acccgcgata acctggacac tttcgacgca accctcgaca ggatctgcgt cgagacctac
     2641 tgctacgagg agaacttcgg gcgcctcgac cgcgccggcg ttctggccag ggcgaaaggg
     2701 ctgaccgtgc ctcaggtcgc gctgatctac gtcctcgctc aaccactgga gatcttcgcg
     2761 ctcgtcggct gcaacgtggg cgaggagttc agggccaacg tcgaagccgg cgccgtagtg
     2821 ctgaccccag aagagattgc ctggctcgag aacggcgaca ccccttagag gaggagagga
     2881 aatcgtccgc gtagtccagg caggactcgg aatctggggc cgtagctggt ctgggctggt
     2941 ggcaggagcc cctggcgtcg agctcgccgc cgccgtagac cccgaccatg tcgacgtggc
     3001 cgcgcttctg gccgcgaggg gcgctaagct gtaggggcgg gtcggggctg gtccgcgagg
     3061 tagagagggt aagaggaggt tggaacgtgg ccagaccggt aacacttttt acgggacagt
     3121 gggctgacct tgcgctggaa gagctcgcca gaaagtgcgg ggagtggggt ttcgacgggc
     3181 tcgagctggc gtgctggggg gaccacttcg acgtagacaa agctctctcg gagagcggtt
     3241 acatagaggg acgctgggag atactgcgca gcaacggcct gaactgctgg gccataagca
     3301 cccacctggt cggccaggcg gtctgcgacc ccatagacgc ccgccacggg gcgacgctcc
     3361 ctcccgacgt gtggggcgac ggcgacccgg aaggcgtgcg ccggcgtgcc gccgagaagg
     3421 ttatggacac tgccagggcc gcagcccgct ttggggtaac ccaggtcaac ggctttacgg
     3481 gatcgagcat ctggcacctt ctctactcgt tcccgcccaa cgacttcgag gagatagagc
     3541 gcggctacca ggacttcgcg gagcggtgga acccgatcat cgacgtcttc gacgaggagg
     3601 gcgtcaggtg gggcctcgag gtgcacccca cggagatcgc ctacgacttc gtgaccaccc
     3661 gcaacgctct cgacgccatc ggcaaccgcg agggcttcgg catcaacttc gatccaagcc
     3721 acttcgccca ccagttcctg gactccgctg cgttcatcga ggagttcgcc gataggatct
     3781 accacttcca cataaaggac agccgcaaga ggttgaacgg ccgcaggagc atactgggaa
     3841 gccacctgga cttcggtagt cccgagcgcg gctgggactt cgtctcccca ggccacggcg
     3901 acgtgaacct ggagggctgc ataagggcgc tgaaccgtat cggctacgag ggtccactca
     3961 gcatcgagtg ggaggactcg gggatggatc gcgagtacgg tgccagggac gcgctggcat
     4021 tcgccagacg cacggacttc gagccatcga cacaggcctt cgacgccgct ttcgcgagga
     4081 aggagtaagc atggagcggg gagagatcgg ggtcgggctc gtcggctaca agttcatggg
     4141 tcgggcccac tccaacgcct accgacaggt cgccagattc ttcgacgtcg accctactcc
     4201 cagaatggcc gctatctgtg ggcgcgacga ggccggcgtg agggaggcgg cggacactct
     4261 gggctgggac tcctacgaga cggactaccg caagctcata gcgcgcgatg atatagatct
     4321 cgtggacgtc gtcacaccgg gcaacacgca ccgcgagatc gtgcttacag ccctcgaggc
     4381 gggcaagcac gtcctttgcg agaagccgct cgccaataat ctctctacag cccaggagat
     4441 gttcatcgcg gcccgcaacg cggggacggt caacatggtc tgcttcaact acaggcgcgc
     4501 ccccgccgta cagcttgcga aaaagctcgt cgaggaagga aggctcgggg agataaggca
     4561 ctggcgggcc acgtatttgc aggacttcat catggaccct tctttccccc tgatctggcg
     4621 cctccagaaa gaggtctcag gctcaggcgc cctcggcgac ataggggccc acatcatcga
     4681 cctggcccac ttcgtggtcg ggcctatctc cgaggtaaca ggcatgacgg agacgtttgt
     4741 agaggagcga cctctcgaag aaacgagtgc tggcggaggg ctctcggcaa gcggtggccc
     4801 ggagatggga gaggtaaccg tagacgatgc cgcagccttt ctggcccgct tcgagaacgg
     4861 ggccataggg accttcgagg ccacgcgctt cgcggccggc cgcagaaacc agaacgcctt
     4921 cgaggtgaac ggctccaaag gaagcgtcgc cttcgacctg gagcgcatga acgagctcga
     4981 ggtgttcttt acggaagacg aaccagacgt ccagggcttc aggaccgtga acgtgacgga
     5041 gcctgaccac ccgtacacgg gggcctggtg gccgccgggc catatcctcg gctacgagca
     5101 cactttcgtc cacaccataa aagacctgat ggacggcata aaggtgggga agagcccggc
     5161 cccgacgttc gaggacggct accggtgcca ggctgtgctc gacgcggtcg aacgcagcgc
     5221 cgctagcggc gaatggagaa gaccggagat ggaagggatc tagacttggc tgaagagccg
     5281 cgcgtaacca tctggaacga gtacaggcac gaaaagagcg acgagcaggt cgcgaagatc
     5341 taccccgaag gcatgcacgc cgtactcgcc gcagcactca ccgaggccgg cttcgaggtc
     5401 catgcggcga ccctcgacga gcccgagcac gggttatccg atgaggtact ggacgctacg
     5461 gacgtgctcc tctggtgggg ccacatggcc cacgacgagg tctctgacga aacagtcgag
     5521 aaggtacacc agcgggtgct tggcgggatg ggcttgatcg tgctccactc aggacacttc
     5581 tcgaagatct tcaagaagct tatgggcacg acctgcgacc tcaaatggcg ccacgacgac
     5641 accgaaagac tctgggtcgt cgcggcaggg catcccataa cggaggggct cg
//
LOCUS       DATMJA010000365         4883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_641947, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000365 DATMJA010000000
VERSION     DATMJA010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641947"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VNT12_08750"
     CDS             complement(<1..675)
                     /locus_tag="VNT12_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HWI46246.1"
                     /translation="MKGVPSSRHLGGQPNRVVVLSGALFVVLISLLFYLKTLAPTVLY
                     CDPTAFPDSVVLQIQAIVLGIPHTTGYPTWVMLTHLFTYLPVGDPAYRANLSSAVYAA
                     AAVLLVYLLCLRLTRRAVPSCVGALLFGLSRDFWSQAVITEVYTLNALFVALIIFLLL
                     LWRDTRDDRYLLLACFSMGLSLTNHLMSGLLLPAGVLFVGLVDRRKLREMRLTLKGAA
                     LFLIALT"
     gene            complement(891..2357)
                     /locus_tag="VNT12_08755"
     CDS             complement(891..2357)
                     /locus_tag="VNT12_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HWI46247.1"
                     /translation="MSRKFTRQEFLGVVGAGIALLNMPGCKEDRQSHHSLPVQPSEVR
                     SFRSRPELSPPAIEITAQAQDTAPGYVFVAPKKGAGQDGPMIVDDQGQLVWFSKNRYA
                     TDFKVQTYKGELVLTWWEGKVVAGHGVGEYVIFDGSYHEIRRVRAGNGYQGDLHEFFI
                     TAEDTALLTAYVPTQTDLSLIGGPEDGAVWEGIAQELNLETGEVLFEWRSLEHVGVEE
                     TYREPLQNPGTPLDYFHINSIDIDFDGNFLISAKGTSAVYKVGRESGEILWRLGGKDS
                     SFEMGPGTRIAYQHDARRQRDGTITIFDNGASPKVHEQSRGIVVELDMDGMSATLVRE
                     FTHPNKLISTSQGNVQVLPNANLFVGWGSAPYFSEFSHEGELLFDARFLGEAQSYRAF
                     RFPWRGDPSDNPAVAVEQGPDDKVTLYVSWNGATEVESWQVLAGSTPNHLKPVGSAPR
                     RGFETTVAVRTAEPYVAVLAKSASGKVLGTTKAVEPED"
     gene            complement(2586..2912)
                     /locus_tag="VNT12_08760"
     CDS             complement(2586..2912)
                     /locus_tag="VNT12_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46248.1"
                     /translation="MPEGGLVQRESRIAEVALALPGPLESYLEDHLMLDPAYLAEWGS
                     VGGAMYGVAQPFWQIGPLHRPHYSDRKRPWCGGSGRRCTPGGGVPAVLSGAMISMGRL
                     LAKLGL"
     gene            2927..3097
                     /locus_tag="VNT12_08765"
     CDS             2927..3097
                     /locus_tag="VNT12_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWI46249.1"
                     /translation="MATTVITADEPGEAVYVILSGSAKVHVIRPDGTEVILAVLGPGE
                     VAGEMSNAISPK"
     gene            complement(3187..>4883)
                     /locus_tag="VNT12_08770"
     CDS             complement(3187..>4883)
                     /locus_tag="VNT12_08770"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWI46250.1"
                     /translation="KEGIPHVIVVNHLDRERTDFGAVVDELRERFGQAVVPLNLPIGR
                     ENDLKGIYGLLSGTSFVDGEQRAEIPEGMEDEVDAAKTQLFEAIAESDDTLLEKYLEG
                     EEITTEEAFDGIRKGIAEGLIIPVLAASAERMIGIDRLLDVIAGSAPSPSDLERWITE
                     AGDEVPCDPEGPFAAYVFKTYVDPYAGRLSVLRIVGGRCRSDEALTNPRTDSSERLGG
                     ISHLVGKERDPVDEAVAGDIIAVAKLRDTATFDTLCKPERPMSFEPVELPEPTAAFAV
                     GAKARGEEEKVFDAIRRMADEDPSLKLERTEATGEDILAGLAQMHVELALERIEHRYG
                     VEVEARPPKVPLMETITGSAQAQGRYKKQTGGRGQFGDARIELSPLPRGSGFEFENGI
                     VGGAIPRQFIPAVEKGIVEAMIGGNIAGYPVVDVKVRLHDGAFHTVDSSENAFKVAGS
                     MAFKNAVEKAHPVLLEPYVKVEILTPAELVGDIMGDLSSRRGRPMGVEQRGERQIIQA
                     EVPQIEMLTYARDLRSITGGRANFHVEQGHYEEVPANLVERVLAANEREEEKEKVG"
BASE COUNT          859 a         1557 c         1530 g          937 t
ORIGIN      
        1 cgtgagggcg atcaggaaca aggccgcacc cttcagcgtg agccgcatct ccctcagctt
       61 gcgccggtcg accagcccta cgaataagac tccggcgggc aagagcaggc cgctcatcag
      121 atggttggtc aaggacaggc ccatcgagaa gcacgcgagc agcagatacc tatcgtctct
      181 ggtgtctctc cagagcagga gcaagaagat aatgagagcc acgaaaagag cgttcagagt
      241 gtagacctcg gttatcaccg cctgcgacca gaagtctcgg ctgagcccga agagtagggc
      301 tccaacgcac gagggcaccg cgcgcctcgt cagccgcagg caaagcaggt agacaagcaa
      361 gacggctgcg gccgcgtata cagcggagga aaggttggcc ctgtaggcag ggtccccgac
      421 cgggaggtag gtgaagagat gggtgagcat cacccacgtc gggtagccgg tcgtgtgcgg
      481 gatgccaagc actatagcct ggatctgcag cacaaccgag tccgggaacg cggtcgggtc
      541 gcagtagagc accgtcggtg caagcgtctt gaggtagaag agaaggctga tgaggaccac
      601 gaagagggcc cccgagagga ccacgaccct gttcggctgc cctcccagat gcctgctgct
      661 cgggacaccc ttcatggacc gcgttttacc accggagcgc gggcctcgtg gcgaagtcgc
      721 tcctttattg gtcggcgcat cccgaagacc cggatgctgc ttgcgttttc gctcgcgcga
      781 ggtcggcttc tggggccttg gctgaacctc cgaaggatac cgagtcgccc tttcgccggg
      841 gtttgtcgtg ttcacaagga tactggcggt gagaatccgc tcgacgctgt tcagtcttcc
      901 ggctcgaccg ccttagtggt gccgagcacc ttccctgaag cgctcttggc cagtacagcg
      961 acgtacggct cggcggtgcg cacggcgact gtggtttcga agccgcgccg gggagcggat
     1021 ccaacgggct tgaggtggtt aggggtggag ccggccagca cctgccagct ctcgacctcc
     1081 gtggcgccat tccaactgac gtagagcgtc accttatcat cgggtccctg ctcgaccgcc
     1141 acagctgggt tgtcgctcgg gtctccgcgc cacgggaagc ggaatgcccg gtaagactgg
     1201 gcttctccaa ggaaacgagc gtcgaagagg agttcgccct catgcgagaa ctcggagaaa
     1261 tacggcgcgc tgccccaacc tacgaataag ttggcgttgg gtagcacctg tacattgccc
     1321 tgggacgtgg agatcagctt gttcggatgg gtaaactccc gcaccagggt agcgctcatc
     1381 ccgtccatgt cgagctccac tacgatcccg cgggactgct catgcacctt aggggaggca
     1441 ccgttatcga aaatggtgat ggtgccgtct ctctggcggc gggcgtcgtg ctggtaggcg
     1501 atccgggtgc caggtcccat ctcgaaggag ctgtccttgc cgccgagccg ccacaggatc
     1561 tcgccgctct cgcggccgac cttgtagacg gcagaggtcc ctttagcgga gatgaggaag
     1621 ttgccgtcga agtctatgtc tatggagtta atgtggaagt agtcgagggg tgtgccgggg
     1681 ttctgcagcg gctcgcggta ggtctcttca acgccaacat gttccaagct gcgccactcg
     1741 aagagcacct ctccggtctc gaggttgagc tcctgggcga tcccttccca caccgcgccg
     1801 tcctccgggc cgccgatcag ggagaggtcc gtttgcgtcg ggacgtaggc tgtcaagagc
     1861 gcggtgtcct cagcagtgat gaagaactca tgcaggtcgc cctggtagcc gttacccgcc
     1921 cggacccggc gtatctcgtg gtaagagcca tcgaagatca cgtactcgcc aacgccgtgg
     1981 ccggccacga ccttgccctc ccaccaggtc agtactagct ctcccttgta ggtctgcacc
     2041 ttgaaatccg tcgcgtacct gttcttgctg aaccacacga gctgcccttg gtcgtcgacg
     2101 atcatcggac cgtcctgccc ggcgcccttc ttcggggcga caaagacgta gccaggagca
     2161 gtgtcttgcg cctgagcggt tatctcgatg gcaggagggc taagctctgg gcgcgagcgg
     2221 aaggatctca cctcgctcgg ctgcaccggg agagaatggt ggctctgccg atcctcttta
     2281 caccctggca tgttcagtag cgcgatacca gcccctacaa cgcctaggaa ctcctgtcgc
     2341 gtgaactttc tgctcaaggt gatgaccgtt gctcggtagt catattcccc aaacgcgagt
     2401 cgaagagggt gcataggcta cagacgaaga gcatgagcaa taggtcattg cttgaggttg
     2461 acacaagttc aggacaatca caagagccgc tggcaactgt aaccatcaaa gcgagacgag
     2521 cttgtaagaa ctcctcaggg cgaagttcgc agaaccctcc ttctacgcac tcgggtggat
     2581 agaagctaca atcctagctt cgccaacagc ctgcccatgg agatcatggc cccgcttagc
     2641 acggcgggca ctccccctcc cggggtgcac cgacgccccg acccgccaca ccagggtcgc
     2701 ttcctatccg agtaatgcgg acggtgcagg ggtcctatct gccagaacgg ctgggccaca
     2761 ccgtacatag ccccgcccac gctcccccac tcggctaagt acgccgggtc tagcatgagg
     2821 tgatcttcga gatagctctc cagggggccg ggcaaggcga gggccacttc tgctatgcgc
     2881 gactccctct gcacgagccc gccctcgggc accccacgcc ccctcggtgg cgaccaccgt
     2941 cataaccgcc gatgagcccg gcgaggcggt ctacgtgatc ctctccggca gcgcgaaggt
     3001 gcacgtgatc cgtcccgacg gcacagaggt gatcctggcc gtgctcggcc ccggcgaggt
     3061 agcgggcgag atgagcaacg ctatctcgcc gaagtgatcg tgcttcgaga gggtcgccac
     3121 tgcgacctcg tcctctcctt taccccgcgc cgcaacccgt ctctaccacg tcagccgcag
     3181 ccttgtctag ccgaccttct ccttctcttc ctccctctcg ttggcggcga ggactctctc
     3241 taccaggttc gcggggactt cttcgtagtg tccttgctcg acgtggaagt tggcgcgacc
     3301 gccggttatg gagcggaggt cgcgggcgta ggtgagcatt tcgatctggg gcacctcggc
     3361 ctgtatgatc tgacgctctc cgcgttgctc gacgcccatc ggccttccac ggcggctgga
     3421 gaggtcgccc atgatgtcgc cgaccagctc cgcaggcgtg agtatctcga ccttcacgta
     3481 cggttccagc agcacgggat gggctttctc gacggcgttc ttgaaggcca tggatcccgc
     3541 gaccttgaac gcattctcag aggagtcgac ggtgtgaaag gcgccgtcgt ggaggcgcac
     3601 ctttacgtca accacggggt acccggcgat gttccccccg atcatcgctt cgactattcc
     3661 cttctccact gcggggatga actggcgcgg gatggcgccg ccgacgatgc cgttctcgaa
     3721 ctcgaagcca gagccgcggg gcagcggtga gagctcgatc ctcgcgtcgc cgaactggcc
     3781 cctgccaccg gtctgctttt tgtagcggcc ctgggcctgg gccgagcctg tgatcgtctc
     3841 catgagaggc accttcggcg gcctcgcttc gacctcgacc ccgtagcgat gctctatgcg
     3901 ctccagggcg agttcgacgt gcatctgggc gagcccggcc aggatgtcct cacccgtcgc
     3961 ttcggtgcgc tccagcttga gcgaggggtc ctcatcggcc atgcgcctta tcgcgtcgaa
     4021 gaccttctct tcctcaccgc gcgccttggc gccgacggcg aacgccgccg tcggctcggg
     4081 gagttcgacg ggctcgaagg acatcggcct ctcgggcttg caaagggtgt cgaaggtcgc
     4141 cgtatcgcgg agcttggcga cggcgatgat gtcccccgcg acggcctcgt ctacagggtc
     4201 gcgctccttg ccgacgaggt gcgagatgcc tccaagtctc tccgatgaat cggtgcgggg
     4261 gttggtgagc gcctcgtccg agcggcaacg accgcccacg atcctaagga cgctcaggcg
     4321 gccggcgtac gggtccacgt aggtcttgaa gacgtaggcc gcgaaggggc cttcagggtc
     4381 gcacgggacc tcatcgcccg cttccgtgat ccaacgctcc aggtcggagg ggcttggggc
     4441 gctaccggcg atcacgtcga gcaggcggtc tatgccgatc atacgctccg cgctcgccgc
     4501 gaggacggga ataatgaggc cctcggcgat ccccttgcgg atcccgtcga aagcctcctc
     4561 ggtggtgatc tcctcgcctt cgaggtactt ttcgagcaac gtgtcgtcgc tctccgctat
     4621 ggcctcgaag agttgcgtct tggccgcgtc tacctcatct tccataccct cggggatctc
     4681 tgcacgctgc tcgccgtcca cgaaggaggt cccgctgagg agcccataga tacccttgag
     4741 gtcgttctcg cgtccgatgg gaaggttcag gggtacgacg gcctgcccga agcgttctct
     4801 gagctcgtcg accacggccc cgaagtccgt gcgctccctg tccaggtggt tgacgacgat
     4861 gacgtggggg atgccttctt tct
//
LOCUS       DATMJA010000366         5104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_287339, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000366 DATMJA010000000
VERSION     DATMJA010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287339"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VNT12_08775"
     CDS             <1..747
                     /locus_tag="VNT12_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI46251.1"
                     /translation="GFDAADFSYRYAIGPLARRRPVLAFDWPGYGGSDKPNNRYDLAY
                     YARILSHLMDSLGIQRAALVGYSMGGGAALSFTLCSPERVEKLVLVASYGLGKDIPHG
                     RLGYFLVHAPLAADLLYTLLRRSRRTLRSGLRRMVCDPRVVSDELVDEARRLLDQPAS
                     GRAFRSFRKSEVGWSGLKTDLSDRLGKVATPTLLVHGERDRVVPVEWARRAHERLPDS
                     ELRVLLGCGHWPPRECPDTFNRVVTDFLAR"
     gene            complement(855..2201)
                     /locus_tag="VNT12_08780"
     CDS             complement(855..2201)
                     /locus_tag="VNT12_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWI46252.1"
                     /translation="MRTLSGPSGPKGHLLGDNLREYARDPLGFLSHCAREYGDIVQLR
                     FMGQTFYLLSHPDLIEYVLVENNRNFTKTKILKRNGRLLGEGLLTSQGEFWRRQRRLA
                     QPAFHRKRITTYGEVMGNFAERSLESWRDGQTVDVHEEMTRLTLEIVAKCLFDADVGV
                     EAKDVGRAMKVALEDFSSQRRLIRIPKNIPTPHNLRFERAARRLDAIVNAIIEERRKS
                     GEEDRGDLLSMLMLAEDESGERMTDKQLRDEVMTLFLAGHETTANALAWTFWLLSSNV
                     AAEADLAEELERVLGGRTPTMADLPRLPYVERVIKESMRLYPPAWVMGREALGECEVG
                     GYRMPAGTTALMSQWVVHRDPRYHHDPQRFDPDRWAAGYENELPRFAYFPFGGGPRQC
                     IGAGFAMTEARLVLATVAQRFGMEFVPGQKVEPYASITLRPKEGIRMTLAARSQGS"
     gene            complement(2433..2633)
                     /locus_tag="VNT12_08785"
     CDS             complement(2433..2633)
                     /locus_tag="VNT12_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46253.1"
                     /translation="MTKSEAARTFSVSRSSVKSYAKLVEEGRPLAPKKRPGSKLKMFM
                     AISSPLYIMVRASQSRCVWVGG"
     gene            complement(3089..4024)
                     /gene="sppA"
                     /locus_tag="VNT12_08790"
     CDS             complement(3089..4024)
                     /gene="sppA"
                     /locus_tag="VNT12_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWI46254.1"
                     /translation="MALGLIGLAIVAVVLILAAGGPTAPAVYAEEYVAGDGASRIAVV
                     PVEGTIAPADNTVGGIQPTTTPEGLADALRQAGQDTSVVAVVIEVNSPGGGVTASDEM
                     HQSILDFRQNTSKPVVVSMGDTAASGGYYISTAADSIVAHETTLTGSLGVIIPLPNFS
                     EAADKYGIKQVVIKSGKYKDMGSSFREMTPEERDILQSFVDDLYAQFVDVISEGRDIP
                     KDRVREIADGRVYSGLQAKKLGLIDSFGGLDEASAIAGNLAGTSDTTVVRYVQQPSFT
                     DTLLARLALEKPQAEQIMDAAGLNLEPKPYYLYRP"
     gene            complement(4275..>5104)
                     /locus_tag="VNT12_08795"
     CDS             complement(4275..>5104)
                     /locus_tag="VNT12_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46255.1"
                     /translation="ARRRAFLRRIGAYLRRDPGSNQLLSFDEVKGALGAVSQVYLGLR
                     EVPVSKIVGSVGRHREFDRAFLPSKPNLGTRWRRIDEIMNRDEELPPVSLYKIGDAYF
                     VQDGNHRVSVARQRGVEMIDAEVTELRSRVPVDSALTARDLLHKLEHRRLLERLPIDR
                     VLPEIEVEFSDVADYRRLATYIEAHGFRVSQLWKRYVSPEEVLRDWYEYGYCPISEMI
                     REERILDAFPDRTELDLYLWIVYHREHLALEARDEKISPQAAKDDILKNVPRRRRQP"
BASE COUNT          888 a         1565 c         1590 g         1061 t
ORIGIN      
        1 gggttcgatg ccgccgactt ttcgtaccgg tacgccatcg gacctttagc tcgccgtcgc
       61 cctgtactcg ctttcgactg gcccggatac ggagggagcg acaagccgaa taaccggtat
      121 gatctcgcct attacgcacg catcctctcg cacctaatgg actctttggg gattcagcgc
      181 gcggcccttg tcggctactc tatgggaggt ggagcggcgc tctccttcac gctgtgttcg
      241 ccggagcggg tcgagaagct cgtcttggta gccagctacg ggcttggcaa ggatatccct
      301 catggaaggc tcggatactt cctcgtgcat gctccgttgg ctgcggacct gctctacacg
      361 ctcttacgcc ggagccggcg cacgctgcgt tctgggttgc gtagaatggt ttgcgacccg
      421 cgggtggtga gcgacgaact cgtcgatgaa gcccgtcgcc ttctggacca gcccgcatcg
      481 ggtcgggcgt tccgctcgtt tcgcaagagc gaggtagggt ggagcgggct gaaaaccgac
      541 ctttcggacc gcttaggtaa agtagccacg ccaaccctcc tggttcacgg cgaacgcgac
      601 cgcgtggtgc ctgtagagtg ggcacgacga gcgcacgaac gacttccgga ttcggagctg
      661 cgcgttctgc tgggctgtgg ccattggccg ccgcgcgagt gcccggacac attcaaccga
      721 gtggttacgg actttctggc tcgctgaacg gaggcatggt ttccggcagc ggtcggggtt
      781 gcttcgacgc ggaaagggtc ggggcgcgtc acagccgcca gtgagaacgg taaccctgag
      841 gtcaagcgct ggagttagct tccctgagag cgcgccgcca aggtcatccg tattccttcc
      901 ttcggtctta gcgtgatgga ggcataaggc tccaccttct ggccaggaac gaactccatc
      961 ccaaaccgct gggccaccgt ggccagaacc agacgagcct ctgtcatcgc gaagcccgcg
     1021 ccgatgcact gtctgggccc cccgccgaac gggaagtacg cgaagcgcgg cagctcgttc
     1081 tcgtacccgg cagcccagcg gtccgggtcg aaccgctgtg ggtcgtggtg gtaacgcggg
     1141 tcgcggtgta ccacccactg gctcatcagc gcggtggtgc cggcgggcat ccggtagccg
     1201 ccgacctcgc actccccgag tgcttccctg cccattaccc aagcaggtgg atagaggcgc
     1261 atcgattcct tgatgacgcg ctctacgtag ggcagccgag gcaaatcggc catggttggg
     1321 gtcctgccgc caagcacgcg ttcgagctct tcggccaaat cggcctcggc ggcgacgttc
     1381 gaagatagaa gccagaaggt ccaagcaagg gcgttggcgg tggtctcgtg gccggccaga
     1441 aacaaggtca tcacctcgtc tcttagctgc ttgtccgtca tccgctcacc gctttcgtcc
     1501 tcggccagca tcagcatgga gaggagatca cccctgtcct cctctccgct cttgcggcgc
     1561 tcctcgatga tggcgttgac gatcgcatcg agcctgcggg ccgccctctc gaagcggagg
     1621 ttgtgcgggg tggggatgtt cttggggatc cggataaggc gccgctgtga agagaagtct
     1681 tccagggcca ccttcatcgc tctccccacg tccttggcct ccaccccaac gtcggcatcg
     1741 aagaggcact tggccactat ctcgagcgtc aagcgcgtca tctcttcgtg gacgtcgacg
     1801 gtttggccat ctcgccagct ctcgagcgag cgctcggcga agttgcccat cacctccccg
     1861 taggtggtta tcctcttgcg gtggaaagcc ggctgggcga gccgtcgctg gcgtcgccag
     1921 aactcgccct ggctcgtcaa gagcccttcg ccgagaaggc ggccgttcct cttgaggatt
     1981 ttggtcttgg tgaagttacg gttgttctcg acgagcacgt actcgattag atccggatgt
     2041 gagagcaggt agaacgtttg acccatgaag cgcagctgta ctatgtcccc gtactctcgg
     2101 gcacagtgag aaagaaagcc taaaggatcc ctcgcgtact cccgcagatt atccccaagg
     2161 aggtgaccct tggggccaga tggaccgctc agtgtcctca ccggatcgtt tgctcctgaa
     2221 ggttgcagag atccatggtc aaagtgatgc ttgcgaaggt cggctcatca ccgagtatac
     2281 cgcgccgagt tgacggagaa cggcatttcg gtgaccttct atcgctggga ctgcctagac
     2341 aaggtgttga tccggaaggc ttcgggtaac gttgtgtgct gcttgagatg acacacggac
     2401 cacccaagct acccaaaggc taccgcctgg acttatccgc cgacccagac gcacctgctt
     2461 tgcgacgccc ggaccatgat gtagagcggc gatgagattg ccataaacat cttgagtttg
     2521 gagccggggc gctttttggg ggcaagcggg cgaccctcct cgaccagctt ggcgtagctc
     2581 ttgaccgagg agcgactgac cgagaaggtg cgagcagcct cactcttggt catgccccga
     2641 cggagggctc ctacgaactt gttccttagg tcttcggagt aggcattcat cgctctataa
     2701 tcgcaggtgg accatctgca ttgcaaaccg ctctagattg tgtgaagaga tcttcttcaa
     2761 ttagtgcgtg tctcagttaa cttggatcct ctatgtcgat cagcaccttg ccttgcgatt
     2821 cttttgcggc gtttgttacg taagccttcc catccaagac gatacgatct cccttttggg
     2881 gcatgagagc gcaatctacc cttcgcacgc tcaatctcag ggcctcgccg cttgcatcgc
     2941 tttcttcggc gtcatgtaca tagaacgttc gggcgtattc ggtagggtct ttcgctcctt
     3001 cagaaacgca gcaattcggc aaggacgaaa agccgaccgg ctgaaaagct tgcgatgggg
     3061 ttgaacccca tcgcaagctg ctccaggctc agggacggta gaggtagtag ggtttcggtt
     3121 ccaggttcag gccagcggcg tccatgatct gctcggcctg cggcttctct agggcgaggc
     3181 gtgccagcag cgtgtcggtg aagctcggct gctgcacgta gcggaccacg gtcgtgtcgc
     3241 tcgtgccggc gaggttgccc gcgatggcgg aggcttcgtc gaggccaccg aaggagtcga
     3301 tgagccccag cttcttcgcc tggaggcccg agtacacgcg gccgtcggcg atctcgcgca
     3361 ccctatcctt gggtatgtcg cggccttcgg agatcacgtc aacgaactgc gcgtacaaat
     3421 cgtctacgaa ggactgtaag atatcacgct cctccggcgt catctcgcgg aaggagcttc
     3481 ccatatcctt gtacttgccg ctctttatga ccacctgctt tatgccgtac ttgtcggcgg
     3541 cctctgagaa gttggggagc gggatgatca cgcccaacga gccggtgagg gtcgtctcgt
     3601 gggcgacgat gctgtcggcc gcggtcgaga tgtagtagcc gcccgaggcc gccgtatctc
     3661 ccatggagac gacgacgggt ttactcgtgt tctgcctgaa atccaggatg ctctggtgca
     3721 tctcgtcgct cgccgtcacc ccaccaccgg gcgagttgac ctctatcacg acggcgacga
     3781 cgctcgtgtc ctgccccgcc tggcgcaagg catccgcgag gccttccggc gtggtcgtcg
     3841 gctgtatccc gcccacggtg ttatcggcag gggcgatcgt accctcgacg ggcacgacgg
     3901 ctatcctgga cgctccgtcg ccggccacgt attcctcagc gtagacggcg ggggctgtcg
     3961 ggccgccagc cgccaggatc aggactaccg ccacgatggc caggccgatc agaccgagtg
     4021 ccatgagacc tcccactaca aagggccacc tgcgccgcct cctgggctcc actgcaggcc
     4081 cctcgggttg ccgggacggc ccgttagaag tagtgtccat tgtgctccct tcgctgctga
     4141 gtgctctcta attttgcgcc aagatcgcgg gcccggtcga cagagcggcc ccgaccacga
     4201 ggattataag gccgatgcag ggtggcgcac gagccggtct gcccggcata gctcgatcgt
     4261 cgccgatgcc aggttcaggg ttggcgtcgc cggcggggga cgttcttcaa gatgtcgtcc
     4321 ttggcggcct ggggtgagat cttctcgtcg cgcgcctcca gcgcaagatg ctcacggtgg
     4381 tagacgatcc agaggtacag gtcgagctcg gtgcggtcgg ggaaggcgtc aaggatacgt
     4441 tcctccctga tcatctctga aatcgggcag taaccgtatt cgtaccagtc cctaaggacc
     4501 tcttcaggtg agacgtagcg cttccagagc tgagagaccc tgaagccgtg cgcctctatg
     4561 taggttgcga gcctcctgta gtcggcgaca tcggagaact cgacctctat ctcgggtagt
     4621 acgcggtcga tgggcagccg ctccagaagg cggcggtgct cgagcttgtg tagcaggtcg
     4681 cgggccgtta gcgccgagtc cacaggcacc ctgctgcgga gctctgtgac ctcggcgtcg
     4741 atcatctcga caccccgctg gcgggctacc gagactctgt ggttgccgtc ctggacgaaa
     4801 taggcgtccc cgatcttgta caggctgacg gggggcaact cttcgtccct gttcatgatc
     4861 tcgtcgatcc gcctccagcg ggtgccgagg ttgggtttgg aaggcaggaa ggccctgtcg
     4921 aattctctgt ggcgtccgac gctaccgacg atcttggaga ctggcacctc gcgcagcccc
     4981 aggtagacct gcgagacggc cccaagagcc cccttgacct cgtcgaagga gagcaactgg
     5041 ttggagcccg gatctcttcg caggtacgcg ccgatccttc gtagaaaggc ccttctgcgg
     5101 gcgc
//
LOCUS       DATMJA010000367         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_346467, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000367 DATMJA010000000
VERSION     DATMJA010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346467"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VNT12_08800"
     CDS             complement(<1..471)
                     /locus_tag="VNT12_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWI46256.1"
                     /translation="MLLVKDSMTREIVTLAPDETVGTALALCRERRIRHLPVLTEGRL
                     VGIVSDRDLRSATPAFGDHARAAALQEILVEDVMVSDVVSVLPDDPIEQAANTMRERR
                     IGSLPVVEGGELVGIITSSDVMNALVYLVGANEPGSRMEIAVPDRPGSLAGAAGV"
     gene            548..934
                     /locus_tag="VNT12_08805"
     CDS             548..934
                     /locus_tag="VNT12_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46257.1"
                     /translation="MPEYLKALLADSATALVRESFTDVSAPWWWERRLGGGIEICQEF
                     DPEAVSREISAKTGREVSEIRQAIAEELGLDDAEPVVLTFEIAGETETRRVAEMLAER
                     SSEPEGLAAGLYNRVEEAVKTNPRDS"
     gene            complement(941..1717)
                     /locus_tag="VNT12_08810"
     CDS             complement(941..1717)
                     /locus_tag="VNT12_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HWI46258.1"
                     /translation="MERMGRARSYAKPWRPPPAVPALEGDEVHVWRVDVGSAYAFRDV
                     LWSFLAGDERQKAADFLFEGDRERFVVSRGVLRTLLGRYLRRHPGSLGFGYNPCGKSH
                     LAGDFGICFSISHSHGLVLLAFARGREVGVDIERVRADLGLEEIAACCFSPREIATLH
                     SLRKDLREEAFFACWTRKEALAKAEGKGLALPLCRFEVTLAPGEPAMMLDTKGELLET
                     TKWTLQELIPDPGYVAALAVEGYGLRLWCWQYTDEFCCDE"
     gene            complement(1719..>2321)
                     /locus_tag="VNT12_08815"
     CDS             complement(1719..>2321)
                     /locus_tag="VNT12_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI46259.1"
                     /translation="AEKLVEIARARGLPVCVYRPSLITGDSRTGAWNTDDFTCKMIRT
                     WVGLGTAPKMDMELNMVPVDYLSRAIVRLSLREESLGRRFHLANPHPVSVNDLVAWIE
                     AFGYPLERVPYDKWRSDLVNPTKGLRQDALYSMAPLLSMSAAADGPALVDKVPEFDCR
                     NTTEALSDTEISCPQVDGELMENYLVNLVRRGFLHSPTGG"
BASE COUNT          439 a          758 c          703 g          421 t
ORIGIN      
        1 gacccccgct gcgccggcca gggaccctgg cctatcagga accgcgatct ccatgcggct
       61 tcccggctcg ttggcgccga caaggtaaac cagcgcattc attacgtcag aggaggtgat
      121 gatgccgacc agttccccgc cctcgacgac ggggagggag ccgatcctgc gctcgcgcat
      181 ggtgttcgcg gcctgctcta tcggatcgtc ggggaggaca ctcaccacgt cgctgaccat
      241 cacgtcctca accaggatct cctgcagcgc ggcggccctt gcgtggtccc cgaacgccgg
      301 ggtggccgag cggaggtccc tgtccgagac gatccccacc agcctccctt ccgtaaggac
      361 tggcaggtgc ctgatgcgcc tctccctgca cagggcgagc gccgtcccca ccgtctcatc
      421 cggcgcgagc gtcacgatct cacgggtcat cgagtctttt acgagaagca tcgaattact
      481 ccttccctca tcgcattgta tagtagtgga tcggcgcagg taagcccgcg gctggtataa
      541 agtccatatg cccgagtatc tcaaagcact tcttgccgac agcgcgacgg ccctggtgcg
      601 tgagagcttt acagacgtaa gcgccccgtg gtggtgggag cggcgcctgg gaggcggcat
      661 cgagatctgc caggagttcg acccggaagc cgtgagccgt gagatctcgg caaagacagg
      721 ccgcgaagtc tccgaaatcc gccaggccat cgccgaggaa cttggcctcg acgacgctga
      781 gcccgtggtc ctcaccttcg agatagcagg cgagaccgag acccgccgtg tcgccgagat
      841 gctagcagag cgttcctcag aaccagaggg cctcgctgca ggcctctaca accgtgtcga
      901 ggaggcggtc aagacaaacc ccagagactc gtagtgggat tcattcgtcg caacaaaact
      961 cgtccgtgta ctgccagcac catagccgca agccatatcc ttcaacggct agggcggcga
     1021 cgtagccggg gtctgggatc agttcttgta aggtccactt cgttgtttcg agcagctccc
     1081 ccttggtgtc aagcatcatc gccggctccc ctggggccag ggtaacttcg aagcggcaaa
     1141 gggggagcgc gagtcctttg ccttcggcct tcgccaaggc ctctttccgc gtccaacagg
     1201 cgaagaacgc ttcctcacgt agatctttcc ggagcgagtg gagcgtggcg atctcccggg
     1261 gtgagaagca gcacgccgcg atctcctcga gaccgagatc ggctcgcacg cgctctatat
     1321 ccacgccgac ctctctgcca cgggcgaaag cgaggagtac caaaccgtgc gagtgggata
     1381 tgctaaagca aattccgaag tcgccagcaa ggtgagattt tccgcaagga ttgtagccga
     1441 agccaagact gcctgggtgc cgtcgcaggt aacgccccag aagagttcgc aacacccctc
     1501 tagagacgac gaaccgttcc cggtcaccct cgaagaggaa atcggcggcc ttctgtcgtt
     1561 cgtcgccggc caagaagctc cacaagacgt cccggaaagc gtaggcggac cctacgtcta
     1621 cgcgccacac gtggacttcg tctccttcca gcgccggcac ggccggtggt ggcctccagg
     1681 gcttggcgta ggatctggcg cgccccattc tttccatgtc agccgccggt gggggagtgc
     1741 aagaagcccc tcctgacgag gttcacaagg tagttctcca tgagctctcc atctacctgc
     1801 gggcacgaga tctcagtatc cgagagcgcc tccgtggtat tcctgcagtc gaactcgggc
     1861 accttgtcga ccagcgccgg cccgtccgcc gccgcgctca tggaaagcag gggcgccatg
     1921 gagtacaggg cgtcttgccg cagacctttc gtggggttga ccaggtccga tcgccacttg
     1981 tcgtacggga cacgctcgag cgggtagccg aatgcctcga tccaggcgac caggtcgttc
     2041 acgcttacgg ggtgggggtt cgccaggtgg aaacgcctgc cgagcgactc ctctcgcagc
     2101 gacaggcgca cgatcgcgcg gctcaagtag tcgacgggga ccatattcag ctccatgtcc
     2161 attttaggcg cggtaccgag gccgacccag gtccttatca tcttgcacgt gaagtcgtcc
     2221 gtgttccaag cccccgtcct gctgtcgccc gtgatcaggg aaggccgata gacgcacacg
     2281 ggtagccctc tcgcgcgcgc gatctccacc aacttctcgg c
//
LOCUS       DATMJA010000368         7051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_228046, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000368 DATMJA010000000
VERSION     DATMJA010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228046"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            878..1579
                     /locus_tag="VNT12_08820"
     CDS             878..1579
                     /locus_tag="VNT12_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46260.1"
                     /translation="MAGMVGFCGSRDLPVSAADSALVSGVVGSVLSGGRGVAFGCAVG
                     ADALVVSSVLALGASSRLCVFAAFGPFSLSWPAACFSAPGASSSVSSVSGVTEALVAG
                     ASVSWWAGGGPSVPLAARLASRSSALVSAVAASGAGRGFVGFVSSPCPAGLGPSPSPS
                     ACFSGSGSGSWGSLALAVGLELPVVVFPVPPSGYGRSPDLPAFWPGSWVPLSGVWAGG
                     FRFVPVPPPQGALFS"
     gene            1729..2160
                     /locus_tag="VNT12_08825"
     CDS             1729..2160
                     /locus_tag="VNT12_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46261.1"
                     /translation="MSKQSVLQELADRAAVELVRVAAARPALSSRCERAAGILANHAV
                     SPRRGLIRVQVRGGELVGYLVRGSGGAVYRVEAKGSWRCSCPDHHGRHRRRDSSRACK
                     HGLACWALWRAAVGASEATPAVVAVETEIRRERVGRERVAV"
     gene            2157..2534
                     /locus_tag="VNT12_08830"
     CDS             2157..2534
                     /locus_tag="VNT12_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46262.1"
                     /translation="MMAVEDRAAVHAHHGAARCLLFLYDRAAEGSGPWSDFDAEAERW
                     NVNYRVSRDELAAMVEGSVRELPAADHLRLHQDAGDFARWGRRGGLRTLALYGRPYFS
                     LLARLRWGLVEVEALVGYRETAR"
     gene            2531..3109
                     /locus_tag="VNT12_08835"
     CDS             2531..3109
                     /locus_tag="VNT12_08835"
                     /inference="COORDINATES: protein motif:HMM:NF042459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HWI46263.1"
                     /translation="MRAATATLRDLRSARDRARAKYLAATRQHSYSRDTAARLALAEV
                     PPNECGIPSSNRTDAMTDHSTPADFWGEPIHVYTRADALADDVLVDVTLTAREASFRI
                     PVALTANVWADVNDLSGPYVLTGQSTEGRLWDLLFMAANAARRPENRDRSEFVYALIM
                     PVGTRNYYRAKCHVGPGDEGEPVVTILRPNED"
     gene            3122..3541
                     /locus_tag="VNT12_08840"
     CDS             3122..3541
                     /locus_tag="VNT12_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46264.1"
                     /translation="MRLIEDYVHAFRGPSGVRSACRVRLYLPDMAKPGEALDDAPLVI
                     VSELADNPGTSVTNAIEQIAAEVMDSHDLRRVPVFVEHYPPESTGGREETFDLVVFAH
                     CEVREVMRGGLWRKEIGPPSWKPLGRRAVEVLVGCPV"
     gene            3614..3958
                     /locus_tag="VNT12_08845"
     CDS             3614..3958
                     /locus_tag="VNT12_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46265.1"
                     /translation="MEVEKIISGLEKLDRAQLACVEAALSRRMQSLGQDAPTSKADEG
                     RPVSGVVEYRPHADGTLQKEVRRYYRKDGTPKEQGPYWYFRYHEGGKQKKLYLGRTDD
                     PEGELARKRTDG"
     gene            complement(4351..5985)
                     /locus_tag="VNT12_08850"
     CDS             complement(4351..5985)
                     /locus_tag="VNT12_08850"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWI46266.1"
                     /translation="MKEQVLDHEEIEWQLEAVDLALVENWLKEHPSAAEVAVVPGGAR
                     ELNDVYYDTEDWRFYRAGYRMRVRRDGESAQATMKALDSAEGALRRRREISEPIDGIK
                     SPKGLSGPVGERVRRLAGTADLRPLFEVRTRRRTFVLRTETPSSGEIVEDGSGNIRQQ
                     NSEQDAIVAEIALDESEIFANGGGSTHLSRVEVEVGSDAHIHDGVGDFVEVLKESLKL
                     RPTLISKFRTGLSVSGLSPEVAPDLGPTKIDATLSAGQVAFAILRRHFGEMLAHEPGV
                     RLGEDPEELHDMRVATRRLRAALKLYSDVLPKRAERYERDLRWVAGALGEVRDLDVHL
                     EGLSEEASSDGEVLEEIAALLRERRVQARRGMLEALDSNRYERLIASFSATLRRGRSP
                     SPTDPILEAAPHLLRERYKKVRKSANRLSEDSPPEHYHDLRKKGKRLRYALEPLQEIY
                     GKQAKKMVKLLKKTQDDLGDHQDLIVASGLMEELGVAGDRPPQVAFSMGTLSEGYGRE
                     ASEIRAGFVGSKSLRTLKDGKQWKKLRKKLEKRAGG"
     gene            complement(6127..6327)
                     /locus_tag="VNT12_08855"
     CDS             complement(6127..6327)
                     /locus_tag="VNT12_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46267.1"
                     /translation="MSASDLKFVIFRDLKDGYRWRLSSASGQTLERSERGHRNKGECA
                     QEVFGLKEDRYPNAKVLDAAIG"
     gene            complement(6575..>7051)
                     /locus_tag="VNT12_08860"
     CDS             complement(6575..>7051)
                     /locus_tag="VNT12_08860"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HWI46268.1"
                     /translation="PSPWGYTSNRETPNSKERQSMSEGSGVAKAREESTRTFFDRFAG
                     RCSEFVSRAPFFTFCVLLVVLWAPTYFLVGNFDTYQLIINTPTTIITFLLVALLQNTQ
                     KRSEQALQHKLDAVADGLADLMEHFAVEDPERRAAKEDLAGDIEDLKAAVGLEEEM"
BASE COUNT         1192 a         2288 c         2196 g         1375 t
ORIGIN      
        1 cagtggacac gtgtgtattg ataagttgcc gaaaaacccc taataggcgt tattcccgga
       61 cgcacaagga cgggctagta tatacgcaag caaactcgta gctggagcag gtcagcggtt
      121 cgagtccgct cgtcggctct caaaaagtgc taatttgcag gtaaaacgta gatgataaag
      181 tgaatgccgg gatgtctccc ggctccatgt actgcaaccc gtactgcaac ccgtttggcc
      241 caagggctta tccgcctggc gttttgaggg cttcaacaac cctctctcag agcgtacatc
      301 tgggcttagc gagagcagct gtcggggctg accagctcac ttcgatggca cgttgctcta
      361 gatccacgtg gctgggtttg ggccgcaggc cgcccctgat gggcggtccc tgccctcgtt
      421 gaggcccgtg cagatgatta gaggtcgctt cgctgttcga ggcgctggtc ttcaaggcgg
      481 cgatctttgc tgtgcagtcg ctcctcagga ccccctgagc ccagcccggc ggtcagcgag
      541 acgtgcgaga gggggtttgg aacctgactg aaaagctagt ttggttatct ccaaaccccc
      601 tctcccgctc tcccccagag atcagatgcc agccgcccaa gctctgaaac ccccgccggg
      661 ccggcaatta tagcgccggc cgtcgtcccc gcaagggcac cccaaaaacg cgtaactcag
      721 cggtaaccac catactccgc gatttttggg gccccaccct tgcgtgtacg cctgatccgg
      781 cgcttatgag tgcccgtctc ggcggcgggg gtttcattcc tcgctccggc ggctggcagc
      841 tttgacagtg tacgcgtccc ctgtggaggt ggtctttgtg gctggtatgg tcggtttctg
      901 tggctctcgt gatctgccgg tgtcggcagc cgactccgcg ctggtctcag gcgtcgtcgg
      961 ctccgtctta tctggcggcc ggggcgtggc ctttgggtgc gccgtcggtg ctgacgccct
     1021 cgtcgtctcc tcggtgctcg ccttgggcgc ctcctccagg ctctgtgtct tcgccgcgtt
     1081 cggtcccttc tccctatcct ggcctgccgc ttgtttctct gctccaggcg cctcttcctc
     1141 cgtctcctcc gtctccggag tgaccgaagc cctcgtcgcc ggcgcctccg tctcctggtg
     1201 ggccgggggc ggtccctcag ttccgctggc agcccgcctc gcttcccgct cctcggccct
     1261 cgtctcggcg gtcgccgcct ccggcgcggg gcgcggcttc gtcgggttcg tctcttcgcc
     1321 ctgcccggcc ggtctcgggc cttctccttc cccctcggcg tgcttctcag gctccggctc
     1381 ggggtcgtgg ggctccctcg cgctcgccgt tggcctggag ctccccgtcg tcgtcttccc
     1441 cgtgccgcca tccggatacg gtcgcagccc cgaccttccc gcattctggc cgggctcgtg
     1501 ggttccgctc tccggcgtgt gggctggcgg ctttcgcttc gttcccgtcc cgcctccaca
     1561 gggcgcgctc ttctcctgat gcaaaagatt ttcctgcaaa acaagaaaaa acatgttggg
     1621 aaattgcctt cggacctgta tatttactgt gcgcgcacaa gtattcagga cgtgtaaatg
     1681 gcctggcggc gctggcatcg ccccaggccc ggcccaagga ggtaagcctt gagcaagcag
     1741 agtgtacttc aggagttggc ggatcgtgcg gcggtagagc tggtgcgggt ggcggctgcc
     1801 cgtccggcgc tttcgtcgcg gtgcgagcgg gcggcgggca tcctggcgaa tcacgccgtt
     1861 tccccgcgcc gggggctcat ccgggtgcag gttcgcggcg gtgagttggt cggctacttg
     1921 gtgcgagggt ccggcggcgc ggtctaccgg gtcgaggcga agggctcgtg gcgctgctcg
     1981 tgcccggacc atcacgggcg gcaccgtcgg cgcgactctt cgcgggcgtg caagcacggg
     2041 ctcgcgtgct gggcgctgtg gcgggcggcg gtgggggcca gcgaagctac ccccgccgtc
     2101 gtggccgtcg agacggaaat ccggcgtgag cgtgtcgggc gcgaaagggt ggcggtgtga
     2161 tggccgtcga ggacagggcc gccgtccacg cccaccacgg ggcggcgcgc tgcctgctct
     2221 tcctctacga ccgggcggct gagggctcgg gtccgtggtc ggacttcgat gccgaggccg
     2281 agcggtggaa cgtgaactac cgcgtctcgc gcgatgagct tgcggcgatg gtcgagggct
     2341 ccgtccgcga gctccccgcc gccgaccacc tgcggctgca ccaggacgcc ggcgacttcg
     2401 cgcgctgggg aaggcgcggg ggccttcgga cgctggcgct ctacggccgc ccttatttct
     2461 cgctgctggc ccgtctccgc tggggcctcg tcgaggtcga ggcgctggtg ggctaccggg
     2521 agacggcccg atgagggccg ccaccgccac cctccgggat ctcaggtcgg cgcgagaccg
     2581 ggcgcgggct aagtacctgg ccgcgacccg ccagcatagc tattcccgcg acaccgccgc
     2641 gcggctggct ctggcggaag ttcctccaaa cgaatgcggc ataccttcga gcaataggac
     2701 agacgcaatg accgaccaca gcacgcccgc cgacttctgg ggcgagccga tccacgtcta
     2761 cacccgcgcc gatgccctgg ctgatgacgt cctcgtggac gtgacactta cggcccgtga
     2821 agccagcttc cgtatccccg tcgccctcac cgccaacgtg tgggccgacg tgaacgacct
     2881 ctccggtccc tacgtgctca ccgggcagag taccgagggg cggctctggg acctgctctt
     2941 catggcggcc aacgccgcca ggaggcccga gaaccgtgac aggagcgagt tcgtctacgc
     3001 gctcatcatg cccgtcggca cccgtaacta ctacagggcg aagtgccacg taggacccgg
     3061 cgacgaaggc gaacccgtcg tcaccatcct gcgtcccaac gaggattgag ggggataacc
     3121 gatgcgcctg atcgaagact acgtccacgc cttccgtgga ccctccgggg tccgctcggc
     3181 ctgccgcgtc aggctctacc tgcccgacat ggcaaagcca ggtgaggccc tcgacgacgc
     3241 cccgctggta atcgtctccg agctcgccga caaccctggt acctctgtca ccaacgccat
     3301 agagcagata gccgccgagg tcatggactc acacgatctc aggcgcgtcc cggtcttcgt
     3361 cgaacactac ccgcccgagt ccaccggagg acgcgaagag accttcgacc tggtcgtctt
     3421 cgcccactgc gaggtcaggg aggttatgcg tggcgggctc tggcgcaagg agattggtcc
     3481 gccgagctgg aagcccctcg gccgccgcgc tgtcgaggtc ctcgtcggat gcccggtgta
     3541 ggcacgcgtt gaccgtccgg caacaacggc gcacaataaa ttgcgggtgt gcgcgcacag
     3601 accgaggagc cccgtggaag tagagaagat catctccgga ttggagaagc tggatcgggc
     3661 gcagctcgcc tgcgtcgagg ccgcgctcag tcgccggatg caaagtcttg ggcaggacgc
     3721 tccaaccagc aaagccgatg agggccgacc ggtctccggg gtggtcgagt accgtcccca
     3781 cgcggacggg acgctccaga aggaggtccg caggtactac cgcaaggacg gcacgccgaa
     3841 ggagcaggga ccttattggt acttccgcta tcacgagggc ggcaagcaga agaagctcta
     3901 cctcggccgc accgatgatc cagagggaga actcgcccgt aaacgaacgg acggatagtg
     3961 gggcgtcgca ataggcatgg tgccagtgag acgatactgg cccgacaggt aagaacatac
     4021 gtcggcgagg agatcggtcg ggtagactgg ttcatacaat aggtggcaga agatagtgcc
     4081 agtctaggag gggacatggt caggatcagg gacgagacgg acctcgacac cgtcaagaaa
     4141 gacctcgttg gcgtggtgag ggacaccgtg cagcccgccc acgtcacaat ttccggacgc
     4201 accagtacgg gctagaacgt ccgcacgcaa actcgtagct ggagcagggg taagcggttc
     4261 gagtccgctc gtcggctctc cattttgtcc ggtttgctcg taaagtcgat aggcggcctg
     4321 gtggacgagg tacaactccc caagcccagc ctaccctccg gcccgcttct ccagcttctt
     4381 ccgaagcttc ttccactgct tgccgtcctt cagggtccgg agggacttgg atccgacaaa
     4441 gcccgccctt atctccgacg cctcgcgccc gtagccctcg gacagcgtgc ccatcgagaa
     4501 ggcaacttgc ggcgggcgat caccggcgac ccccaactcc tccatgagcc cagaggcgac
     4561 gataaggtcc tgatggtcgc ccagatcgtc ctgtgtcttc ttgaggagct tcaccatctt
     4621 cttagcctgc ttgccgtata tctcctgcag gggctccaag gcgtagcgca gtcgcttgcc
     4681 tttctttctt agatcgtggt agtgctctgg gggcgaatcc tcgctcagcc tattggcgct
     4741 cttgcgaacc ttcttgtaac gctccctgag aaggtgcggc gcagcctcca ggatgggatc
     4801 ggtggggctc gggctacggc cgcgacgcag tgttgcagaa aagcttgcga taaggcgctc
     4861 gtagcggttg gagtcgagtg cctcaagcat gccccttctg gcttggaccc gccgctccct
     4921 caggagagct gcgatctcct ccaggacctc cccgtcgcta gatgcctctt cagagagccc
     4981 ttcgagatgc acgtccagat cccgcacctc tcccagagcc cctgcgaccc atcgcaggtc
     5041 tctctcgtag cgttctgctc gcttgggcag gacgtctgag taaagcttga gcgcggctct
     5101 aaggcggcgt gtggccacgc gcatgtcgtg gagctcctca ggatcttcgc ccagccggac
     5161 gcctggctcg tgcgccagca tctccccaaa gtgacgccta aggatggcga aggcgacctg
     5221 gccagcagac agagtagcgt ctatcttggt gggaccgaga tccggagcca cctcggggct
     5281 caagccggat accgatagcc ccgtcctgaa cttggagatc agagtcggac ggagcttaag
     5341 agactcctta agcacctcga cgaaatcccc gacaccgtcg tggatgtggg cgtcagaacc
     5401 aacctcgacc tccacacgac tcaggtgggt actcccccct ccgttagcga agatctctga
     5461 ctcgtcgagt gcaatctcgg cgacgatggc atcttgctcc gagttctgtt ggcggatgtt
     5521 gcccgagccg tcctcgacga tctcccctga ggagggtgtt tcggtacgaa gcacgaaggt
     5581 gcgccttcgg gtacgaacct caaagagcgg gcgcagatct gcggtgccgg cgagtctcct
     5641 gacgcgttca ccgaccggcc cggagagccc ctttgggctc ttgatgccgt cgatgggctc
     5701 ggagatctcg cggcgccggc gcagagcacc ctcggcggag tccagggcct tcatcgtcgc
     5761 ctgtgcgctc tcgccatccc ggcggacgcg catacggtag ccagcccggt agaaacgcca
     5821 gtcctccgta tcgtagtaga catcgttcag ctctctcgcc cctccgggta cgacggccac
     5881 ttcggcggca gaaggatgtt cctttagcca attctcgacc agtgcgagat cgaccgcctc
     5941 gagctgccac tcgatctcct cgtggtccag cacttgctct ttcatagagt tggaaatcac
     6001 cctagaaatc acctcaacta atgtcgtggg taagggaaga ggctatcgta gcatccgccg
     6061 aaaatatgtc taacgagtgc cggcgctcgt gggtgagctt cgcaagtagc gaggcctgcg
     6121 tgcaattcag ccgatggccg cgtctagcac cttggcatta gggtagcgat cttctttgag
     6181 gccaaacact tcctgggcgc actcaccctt attgcggtgt cctctctcag agcgctctag
     6241 agtctgtcct gatgcggagc taagcctcca gcggtagccg tccttcagat cgcggaagat
     6301 aacgaacttc aggtcgctag cagacacaaa tattccctcc tacaagtagt tgtcccattc
     6361 tccagtcagc agcttacccg cactgccaag gtgctaaaac gctcagccaa aggtgtaggt
     6421 ttaaccctct gggccctcag atgtacgcaa cggaagccca ttcaccgaag tgctcggaag
     6481 gtgaattctc cgacgttggc actttggtgg cgcaaaaggc gccgaaacag cgcttctggc
     6541 atccaaatgg gcttcctcaa aacactaaca aagctcacat ctcctcttcg aggcccaccg
     6601 cggccttcag gtcctcaatg tcgcctgcca ggtcctcctt tgcagcccgc cgctcgggat
     6661 cctccacggc gaagtgctcc atcaggtctg ccaggccgtc tgccacggcg tcaagcttgt
     6721 gctggagggc ctgctcgctg cgcttctggg tgttctgcaa gagcgccacc agcaagaagg
     6781 tgatgatcgt ggtgggggtg ttgatgatga gctggtaggt gtcgaagttg ccgaccagga
     6841 agtaggtggg cgcccatagc accactagca gcacgcagaa ggtgaagaaa ggcgccctgc
     6901 tgacgaactc agagcaccga ccggcgaacc ggtcgaagaa cgtcctggta ctctcttctc
     6961 tggccttggc tacgccgctg ccttcgctca tgctctgccg ctccttcgag ttcggggtct
     7021 ctctgtttga ggtatatccc caaggcgaag g
//
LOCUS       DATMJA010000369         3817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_464756, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000369 DATMJA010000000
VERSION     DATMJA010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464756"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VNT12_08865"
     CDS             <1..1221
                     /locus_tag="VNT12_08865"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HWI46269.1"
                     /translation="NSHIRRSRGMQAVMETYKRLGIAPTGGEGSWLFDSEGNRYLDFI
                     GGIATNSLGHGHPELIGAIQEQAGELIHCSNLYEIPLQAEVAEMLSGATDFDRVFFCN
                     SGAESVEAAIKLSRKYAHDTYGPNKHEILTFSKSFHGRTYGGLTATAQPALQDGFGPM
                     LPGMVHASFGDLEAAQALVGPQTAAVLVEPIQGEGGINEPPEGFLEGLRGLCDEHGAL
                     LIFDEVQTGAGRTGYLYAYQGTDVVPDVLTSAKGLGGGFPVGAMMVKEEYAALGPGNH
                     GSTFGGNPLAMAAVKAVLGVVNDPAFLEEVRFKGTVLKNALEKLAEQIPGAEVRGEGL
                     LLGLDLGNAGLAGEVFEHCLDQGVLVNLVGGTTIRMAPPLTVSRTEVRAALDTFRGSV
                     YQAVEETVPEAAVA"
     gene            1224..2180
                     /gene="argF"
                     /locus_tag="VNT12_08870"
     CDS             1224..2180
                     /gene="argF"
                     /locus_tag="VNT12_08870"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWI46270.1"
                     /translation="MMIPDLQNASPPPSPLSGRDCLTLGEFIPEEMSLILDEAGRIKA
                     LQKSRIPYRPLRGRTLAMVFQKPSNRTRVSFEVGMYQLGGHALHLSPEEIQIGKRETP
                     SDTGRVLARYIDAIMVRTFDHGDLEELADAADVPVVNGLSDTHHPCQAIADLMTVREE
                     FGSVEDINISYVGDGNNVAHSLAIACALSGAELTIAHPKGHGPEKEIVELAESLGAAP
                     HLTEDPDEAVMGARVVYTDVWASMGQETEAEERKEKFAPYQVDEELMALAAEDAIFLH
                     CLPAHRGQEVTAGVIDGPRSRVFDQAENRLHAQKALLYLLMG"
     gene            complement(2273..2476)
                     /locus_tag="VNT12_08875"
     CDS             complement(2273..2476)
                     /locus_tag="VNT12_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWI46271.1"
                     /translation="MAQGTVKWFSDEKGYGFISPDDGGEDLFVHHTGIAGEGFKTLDE
                     GAKVTYEATQGRKGMQAENVTPA"
     gene            complement(2718..>3817)
                     /locus_tag="VNT12_08880"
     CDS             complement(2718..>3817)
                     /locus_tag="VNT12_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HWI46272.1"
                     /translation="TGYMERGGANLHGVFPTSTETEEILADARMAAADFLGAASDEIS
                     FGANMTTLTFEVSRALARGWDRDSEIVVTELDHRANVDPWLIAAAEQGARVRWIRVDP
                     ETLTLDENDLEQNITGRTKLVAIGLASNAVGTVNDVAAVAERANSAGALVAVDAVHAA
                     PHLPLDRDAIGADIITCSAYKFFGPHVGLTAIRRDLFEMMGVYRLDPAPDYIPDKLET
                     GTQNHEGIAGVKGALDFISSHGEGTSQREKLTSAMKAVEEQEAALAENFRRALRDIPG
                     VKLYAAPDGVRKTPTIAFTVQGKTPEEVCLQMLEHGFFIAAGDFYASTLAAKLGIGDS
                     GGFIRAGLAPYNTEEEVERFVEALERIIRET"
BASE COUNT          707 a         1217 c         1181 g          712 t
ORIGIN      
        1 aactctcata tccgaaggag tcgcggcatg caagcagtga tggagaccta caaacgtctg
       61 gggatagcgc caacgggagg tgagggaagc tggctcttcg atagcgaggg taaccgttac
      121 ctggacttca taggcgggat cgccacaaac tccctgggtc acgggcaccc ggagctcatc
      181 ggggctattc aggagcaggc cggggagctc atccattgct ccaaccttta tgagatccca
      241 ctgcaggccg aggtagcaga gatgctctcc ggtgcgacgg acttcgacag ggtcttcttc
      301 tgcaactcag gagccgagag cgtagaggcg gcgatcaaac tctcacgcaa gtacgcccac
      361 gacacctacg gtcccaacaa acacgagatc ctgacgttct cgaagtcctt ccacgggcgc
      421 acctacggcg gccttactgc cactgcccag cccgcgctac aggacggctt cggcccgatg
      481 ctgcccggga tggtacacgc ctccttcgga gatctcgaag ctgcacaggc gctggtcggt
      541 ccccagaccg cagccgtgct cgtcgagccg atccagggcg agggcggtat aaacgagcca
      601 ccggagggct ttctcgaagg gctacgcggg ctttgcgacg agcacggggc gctcctcatc
      661 ttcgacgagg tccagaccgg cgcgggacgc acgggatatc tgtatgccta ccaggggacg
      721 gatgtcgtcc ccgacgtgct caccagcgcc aaggggctgg gtggcggttt ccccgtaggg
      781 gcgatgatgg tcaaggagga gtacgccgcg ctcgggccgg gaaaccacgg ctccaccttc
      841 gggggcaacc ccctggcgat ggccgccgtg aaagcggtgc ttggcgtggt gaatgatccg
      901 gccttccttg aagaagtacg tttcaagggg accgtgttga agaacgctct cgaaaagctg
      961 gcggaacaga tccccggtgc ggaggtgcgg ggcgaagggc tcctgctcgg gctcgacctt
     1021 gggaacgccg gcctcgccgg agaggtcttc gagcactgtc tggatcaggg cgtcctcgtc
     1081 aatctggtgg gcggcacgac catccgcatg gcgccgccac tcaccgtaag cagaacggag
     1141 gtacgggctg cgctcgatac ctttcgaggc agtgtttacc aggctgtaga ggagacagtg
     1201 cccgaggcgg cggtagcgtg agcatgatga tccctgacct ccagaacgct tcgcctcccc
     1261 cttcccccct atcgggtcgc gactgcctga cactcggaga gttcatccca gaggagatgt
     1321 ccctgatcct ggacgaggcc ggtcgcataa aggcgctgca gaaaagtcgc atcccctaca
     1381 ggcccctgcg cggtcgtacg ctcgcgatgg tcttccagaa gccatccaac aggacccgcg
     1441 tctccttcga ggtaggaatg taccagctag gcggccacgc gctccacttg agccccgagg
     1501 agatccagat cggcaaacgc gagacgcctt ccgacacggg ccgcgtcctc gcccgctaca
     1561 tcgacgccat catggtccgc accttcgacc acggggacct ggaggagctc gccgacgcag
     1621 ccgatgtccc cgtagtaaac ggtctctcgg acacccacca cccttgccag gccatcgccg
     1681 acctcatgac cgtgcgcgag gagttcggtt cggtcgaaga catcaatatc tcctacgtcg
     1741 gcgacggcaa caatgtggcc cactccctcg ccatagcctg cgccctgagc ggcgcagagc
     1801 tcaccatcgc ccaccccaaa ggacacggtc cggaaaaaga gatagtcgag ctggcagaat
     1861 ctctgggcgc tgcaccacac ctcaccgaag accccgacga ggccgtcatg ggcgccaggg
     1921 tcgtctacac cgacgtatgg gccagtatgg gacaggagac cgaggccgag gagcgtaagg
     1981 agaagttcgc cccctaccag gtcgatgagg aactaatggc cctcgccgca gaagatgcca
     2041 tcttcctgca ctgtctgccg gcgcacaggg gtcaggaggt cacggccggg gtcatagacg
     2101 gtccgaggag ccgcgtcttc gaccaggccg agaaccgtct gcacgcccag aaggcgttgc
     2161 tctaccttct gatgggatga tcctaggtaa ggacggttcg cgaaccgccc ctacgcacca
     2221 caaaggccga ggtttgtacc cccggccttt gcgttctctt gcttctcggg ttctacgccg
     2281 gagtaacgtt ctccgcctgc attcccttac gcccctgggt cgcctcgtac gttaccttgg
     2341 cgccctcgtc gagagtcttg aagccctcgc ctgctatgcc cgtgtggtgc acgaagaggt
     2401 cctcaccccc gtcgtcgggg gagatgaagc cgtagccctt ctcgtcgctg aaccacttta
     2461 ccgttccttg ggccatatac cggagcccac ctttcgtgtt cctgggctcc actaaacgaa
     2521 aaagccaccc tctatctcac ggggtggcgc tccgtctact gtaaacccac cttacgttac
     2581 agagaaagat tataccagaa tctcgaagag ggttgtatca ggccagcggc ctcccggacg
     2641 tcctcggggg tggggttcat caggaaggag ccgccttcga tctccccttg acgatcccaa
     2701 gcttcgtggg ccggatctca cgtttcgcgt atgatcctct ccaacgcttc gacgaacctc
     2761 tccacctctt cttcggtgtt gtacggagcg aggccggcgc ggatgaaccc accgctgtcc
     2821 ccgataccga gctttgcggc gagcgtcgag gcatagaagt cgccggcggc tatgaagaaa
     2881 ccgtgctcga gcatctgcaa gcagacttcc tcaggcgtct ttccctgtac ggtgaaggcg
     2941 atggttgggg tcttgcggac gccatcaggg gcggcgtaga gctttacgcc ggggatgtct
     3001 ctgagtgctc tgcggaagtt ctcggcgagt gcggcttcct gctcttcgac ggctttcatg
     3061 gcgctggtga gcttctcccg ctgcgaggtg ccttcaccgt gggaggagat gaagtccagg
     3121 gcgcccttta cgccagcgat accctcatgg ttctgggtac ccgtctccag tttgtctggg
     3181 atatagtccg gcgccgggtc gagcctgtag acgcccatca tctcgaacag gtcgcgcctt
     3241 atggccgtca aaccgacgtg agggccgaag aatttgtagg ccgaacaggt gatgatgtcg
     3301 gcccctatag cgtcgcggtc caaggggagg tgcggggcgg cgtgcacggc gtcgacggct
     3361 acgagggcgc ctgcgctgtt ggctctctcg gcgacggcgg ccacgtcgtt cacggtaccg
     3421 accgcgttcg aggccaggcc gatagctacg agtttcgtac gcccggtgat gttctgttcc
     3481 aggtcgttct cgtcaagcgt aagggtctcg ggatctaccc tgatccagcg cactctcgcc
     3541 ccctgctctg ctgcggcgat caaccaggga tccacgttgg cacggtgatc gagctctgtg
     3601 acgacgatct ccgaatctct gtcccaccca cgggcgagcg cgcgggagac ctcgaaggtc
     3661 agtgtagtca tgttggcccc gaaggagatc tcgtcggacg cggcaccgag gaaatccgcc
     3721 gccgccatgc gggcatctgc cagtatctcc tcggtctccg tgctcgtcgg gaagacgccg
     3781 tggaggttcg ccccgccgcg ctccatgtag cccgtga
//
LOCUS       DATMJA010000370         5891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_109717, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000370 DATMJA010000000
VERSION     DATMJA010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109717"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VNT12_08885"
     CDS             <1..260
                     /locus_tag="VNT12_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HWI46273.1"
                     /translation="TESVKGLETRLERVESKLDRLLAALEGGSQDGALAAETNGSGVE
                     VMATDAARRKAREMGVNLAEVVETGADGQVTVEDVRKKGES"
     gene            261..1361
                     /gene="phaC"
                     /locus_tag="VNT12_08890"
     CDS             261..1361
                     /gene="phaC"
                     /locus_tag="VNT12_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HWI46274.1"
                     /translation="MTETANYGAVPTSGDAGIPVDVENFFDKYSRGMKIVVEGAQADT
                     GQTPKETVWTKNKAKVYHYAPNAQKKHPVPILMVYALINRPYILDLMPGNSFIEYLVG
                     EGFDVYLLDWGVPGDEDKDLTFENYVLDYLPRAVKKVLRHSHSDELTMLGYCMGGTMS
                     AMYASLFPENLKNLILMTAPVEFPEGEMGLYSLFTSEKYMNPGLLADAFGNIPGDLID
                     TGNRMLKPVTNYVGTYVNMWERIFEEKPMETWLAMNKWVNDGPPFPGAAFRQWITEFY
                     QQNKLARGEMVLRGRRIDLSNIRVPLLNIAGKKDHICTLPQAEATMNLISSEDKEFFV
                     LDAGHVGLMTGRGAKKGLWPKVSDWLGTRSGG"
     gene            1495..1932
                     /locus_tag="VNT12_08895"
     CDS             1495..1932
                     /locus_tag="VNT12_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWI46275.1"
                     /translation="MQEQSGQLERLGEDWAAAELRGDTLTLGRIFADDFVAVGPRGFM
                     LSKEQWLSRHDSGNLTYEAFEWDEVTVRVHGDAAVMIGRETASAVYEDREVRHEIQDQ
                     FRATLVFVEEQGRWLLLGLQLSPIAGPPAGRPVQNPNEAEERS"
     gene            1929..2810
                     /locus_tag="VNT12_08900"
     CDS             1929..2810
                     /locus_tag="VNT12_08900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWI46276.1"
                     /translation="MKLGVQLNSFDWSGGPEQFGKNLADIGRVAEEGGFDRIGVADHL
                     WQHPIMNGPEANEPECYTTLAFLAANTERIRLTAMVSGVHFRHPAVLVKAVSTLDVLS
                     GGRACFGVGSGHYPEESRGLGIPFPPQKERFEMLEETLQIALRMWSGEHGDERPFEGE
                     YYTLDRPLNLPQSLSRPHPPIMIAGEGEQKTLRLVARYADACSLRPGPQVPGKLAVLR
                     RHCDAEGRDYEDIEKTCAFAFDVGESGEKVEELIGQLRWLSGMGIETVIGFVPGVDRI
                     TPLEIIGRDVIPAVADL"
     gene            2850..3281
                     /locus_tag="VNT12_08905"
     CDS             2850..3281
                     /locus_tag="VNT12_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46277.1"
                     /translation="MIESTGDPGSAMKFVVGILSAVYAVTFFCGALLHLGVQIPLGAA
                     VLAEPTILPAAIVESLCGLALSFGAFAVFARWNRAWTAVFAAHAFALGGVLLGVTALA
                     IGAGPSTDLNTMYHRVMLVGLVAVLALLLTPAGSAAFRREA"
     gene            3339..4559
                     /locus_tag="VNT12_08910"
     CDS             3339..4559
                     /locus_tag="VNT12_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWI46278.1"
                     /translation="MNERVKGDNPQGDSSLSRFPRGGRLLGPLFGLVFLIYPVRAVLT
                     SDPTPARVSLALVGAALFAGVFLWLMWMQEPLWSASAESSEVRKRRATIAFLAVLALA
                     LNLGLGSEWRVLFFHVNVAAGIMLLSRDAYVAIVGLAATTLALGFGSGMAWLALPTAA
                     IGLWATAFVRQVAAVAELRSAREELARLAVSEERLRFARDLHDLLGHSLSLITLKSEL
                     AGRLLPTAPEKAATEVHDIEDVARQALREVREAVAGYRRPTLDEELAGASEMLEAAGI
                     SCQIENQTGVLPKAVDAVLAWAVREGTTNVIRHSSAKHCRIVLARENEEIYAEITDDG
                     GGFQKESGQDSGSGLSGLTERVATFAGRVETDSLPNGGFRLRVVLPARNAVERPAWPE
                     PEFDDSFTGEDRRR"
     gene            4556..5164
                     /locus_tag="VNT12_08915"
     CDS             4556..5164
                     /locus_tag="VNT12_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI46279.1"
                     /translation="MTVRVLLAEDQAMVRGALSALLTLEDDIEIVAEASRGDEVLPAA
                     LDTLPDVALLDIEMPGGDGLEAAAVLHERLPSCLVVILTTFGRSGYLRRAMESGAVGF
                     LLKDAPASELAEAIRRAVKGERVVDPALAAAALSEGDNPLTEREREVLAASEGGATIE
                     DIAARLYLSEGTVRNYLSTAIKKLGARNRVEAARLAEKKGWL"
     gene            complement(5192..>5891)
                     /locus_tag="VNT12_08920"
     CDS             complement(5192..>5891)
                     /locus_tag="VNT12_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HWI46280.1"
                     /translation="TEVGDTGVFIDEPQIEFMEQQMLERGYLDNRKMANMFNLLRSND
                     LIWSNVVNNYLLGQKPPAFDLLYWNSDGTRMARDAHSFYLRNTYLENNLIEPGKVEIK
                     GRPIDLSRIEGDIYAVGAEKDHIVPWASAWKISRLANGTTRFTLANSGHIAGMINPPS
                     KGKGRYWATENGGAFDAPADWRENAREHEGSWWEDWSKWLDAHSGAMVTPPDTGSENY
                     PPIEDAPGTYVKEK"
BASE COUNT         1100 a         1780 c         1941 g         1070 t
ORIGIN      
        1 cgaccgagtc cgtcaagggg cttgagacac ggctcgagcg ggtcgagagc aagctcgaca
       61 ggctgctcgc ggcgctcgag ggcggaagcc aggacggcgc gttggcggcg gagaccaatg
      121 ggtctggcgt cgaggtcatg gccacggacg ccgcaaggcg caaagcgcgt gagatgggcg
      181 tgaacctggc tgaggtagtc gagaccgggg ccgacggcca ggtcaccgtc gaggacgtgc
      241 gcaagaaggg agagagctag atgaccgaga cggccaacta cggcgcggtt cctacctcgg
      301 gcgacgccgg cataccggtg gacgtagaga acttcttcga caagtacagc cgcgggatga
      361 agatcgtcgt cgagggcgca caggccgata cgggccaaac tccaaaggag acggtctgga
      421 ccaagaacaa ggcgaaggtc taccattacg cccccaacgc ccagaagaag catcctgtcc
      481 caatactcat ggtctacgcc ctcatcaaca ggccttacat cctcgacctc atgccaggca
      541 acagctttat cgagtacctt gtcggcgagg gcttcgacgt ctacttgctc gactggggcg
      601 tccccggcga cgaggacaag gacctcacgt tcgagaacta cgtcctcgat tatttgccga
      661 gggctgtaaa gaaagtgctc aggcactcgc actccgacga actcaccatg ctcggctact
      721 gcatgggcgg cacgatgagc gccatgtacg cctcgctgtt tcccgagaac ctcaagaacc
      781 tcatcctcat gacagccccg gtggagttcc ccgaaggcga gatgggcctc tacagcctgt
      841 ttacgagcga gaagtacatg aaccccggtc tgctcgccga cgccttcggc aacatcccgg
      901 gcgacctcat cgatacgggt aacaggatgc taaagcccgt gaccaactac gttggcacct
      961 acgtcaacat gtgggagcgc atcttcgagg agaagccgat ggagacctgg ctcgcgatga
     1021 acaagtgggt caacgacggg cctcccttcc ctggtgcggc gttcaggcag tggatcacgg
     1081 agttctacca gcagaacaag ctggccaggg gcgagatggt cctcaggggc cgccgcatcg
     1141 acctctcgaa catcagggtc ccgctactca acatcgccgg caagaaggac cacatctgca
     1201 ccctcccgca ggccgaggcc acgatgaacc tcatcagcag cgaggacaag gagttcttcg
     1261 tcctcgacgc cggccacgtc ggcctcatga ccggccgcgg cgccaaaaag ggcctctggc
     1321 ccaaggtcag cgactggctc ggcacccgct cgggcgggta gcggaagggc tacccttcca
     1381 aaccccgcta tgacattcgt cacccggaaa ccacgacgat cttcactacc tgccgcaaag
     1441 gtgcgctcat acactcctct gtagaaacgt gaaggagctt cagaggaggt acttatgcag
     1501 gagcaaagcg ggcaactgga gaggctagga gaggactggg ccgcagccga gttgcggggg
     1561 gatacgctca cgctcggtcg tattttcgcg gacgacttcg tcgccgtagg accacgcggg
     1621 ttcatgctga gcaaagagca gtggctctcc aggcacgact ccggaaatct cacctacgaa
     1681 gccttcgaat gggatgaggt aacggtccgg gtccacggag acgccgctgt gatgatcggc
     1741 cgggagacgg ccagtgcggt gtacgaagac agggaggtaa ggcatgagat ccaggaccag
     1801 ttccgcgcga cgctcgtctt cgtagaggag cagggacgct ggctgttgct cggcctgcaa
     1861 cttagtccca tcgccggacc ccccgcagga cgtccggtgc agaacccgaa cgaggcggaa
     1921 gaacggtcat gaagctcgga gtgcaactca acagcttcga ctggagcggt ggaccggagc
     1981 agttcggaaa gaacctcgcg gacatcggga gagttgcgga agagggtggc ttcgaccgta
     2041 tcggggtggc cgaccacctg tggcagcacc cgatcatgaa cggcccagag gcgaacgaac
     2101 cagaatgcta cacgacgctc gctttcctgg cggcgaacac ggagcggata aggctcacgg
     2161 cgatggtgtc tggggtgcac ttccgccatc ctgccgtcct tgtaaaggct gtcagcaccc
     2221 tggatgtgct ctccggcggg cgggcgtgct tcggcgtagg ttctggccac tacccagagg
     2281 agtcgcgcgg gctggggata cctttccctc cgcaaaagga gcgtttcgag atgctcgagg
     2341 agacgctcca gatagcgctg cggatgtgga gtggcgagca tggtgacgaa cgtcccttcg
     2401 agggtgagta ttacactctg gatcgtccgc tcaacctccc gcagagtctg agcagacccc
     2461 accctcccat catgatagcc ggcgagggag agcagaagac gttgcgcctg gtcgcacgtt
     2521 acgccgacgc ctgcagcctc cgacccggcc cgcaggtgcc cggcaagctc gctgtgttgc
     2581 gcaggcactg cgacgccgaa ggcagggact acgaggacat agagaagacc tgtgctttcg
     2641 ccttcgacgt cggcgagagt ggtgagaagg tagaggagtt gatcgggcag ttgcgctggc
     2701 tctcgggcat gggcatagag acggtgatcg ggttcgtgcc aggcgtggat aggatcacac
     2761 cgctcgagat aatcggtaga gatgtgatac ctgctgtcgc ggatctttga cggcgggaaa
     2821 tggttttgtt cgcgaggagg gaagctacga tgatcgaatc aactggagat cccgggagcg
     2881 caatgaagtt tgtggtcggt atcctttccg ccgtctatgc ggttacgttt ttctgcggcg
     2941 ctctgttgca tctcggcgtg cagattccgc tcggggccgc cgtactcgcc gaacccacga
     3001 tactcccggc tgcgatcgtg gagagcctct gcgggttggc gctgtcgttc ggcgctttcg
     3061 cggtgttcgc acgctggaat cgggcctgga ccgcggtctt cgccgcccac gccttcgccc
     3121 ttggaggcgt gttgctcggc gtgaccgcgc tcgctatcgg ggcaggtcct agcacggacc
     3181 tgaacacgat gtaccaccgc gtcatgcttg tcgggctcgt tgctgtcctg gcccttctgc
     3241 tgactccagc aggaagcgca gcgttccgtc gcgaggcgtg agcaatagta atacaatcgc
     3301 gttgaatgcg gggggctcct cagggagttc gagatctcgt gaacgagaga gtaaagggcg
     3361 acaaccctca gggcgatagc agcctctccc gcttcccgcg tggcgggcgg ttgctaggtc
     3421 cgctgttcgg attggtcttt ctgatctacc cggtcagggc cgtcctcacg tcagacccta
     3481 cgcccgcacg ggtatcgctt gctctagttg gggctgcact attcgccggt gtgttcctgt
     3541 ggctgatgtg gatgcaagaa ccgctctggt ctgcatccgc agagtcctca gaggtacgca
     3601 agcgccgggc tacgatagcg ttcctcgctg tgcttgcgct cgcgctcaac cttggcctgg
     3661 gcagcgagtg gcgcgtgctc ttcttccacg tcaacgtcgc cgcggggata atgcttctga
     3721 gcagggacgc ttacgtggcg atcgtgggtc tggcggccac cacgttggcg ctcggcttcg
     3781 gctctggtat ggcgtggctc gcgctgccga ccgccgccat cggcctctgg gctacggctt
     3841 tcgtccgcca ggtcgccgcc gtcgccgagc tgcggtcggc gagggaagag ctcgcccgtc
     3901 ttgccgtcag cgaagagaga ttgcgcttcg ccagggatct gcacgacctc ctcggccaca
     3961 gcctctccct catcacactc aagagcgagc tggccggcag gctccttccc acggcgccgg
     4021 agaaggcggc gacagaggtt catgacatcg aagacgtggc gcgtcaagcc ctgcgcgagg
     4081 tccgcgaggc cgtcgccggc taccggaggc ccacgctcga cgaggagctc gccggtgcca
     4141 gcgagatgct cgaagcggca ggcatctcct gccagataga gaaccaaaca ggcgtgctgc
     4201 cgaaagcggt cgacgccgtc ctcgcctggg cggtgcgcga ggggacgacg aacgtgatcc
     4261 ggcacagcag cgcgaaacat tgccggatcg tcctggcgcg cgaaaacgaa gaaatctatg
     4321 ccgagataac ggacgatgga ggtggctttc agaaagagag cggacaggac tcgggcagcg
     4381 gcctctcggg actcaccgaa cgtgtggcaa ctttcgccgg gcgcgtggag accgattcgc
     4441 tgccgaatgg cgggttccgc ctgcgcgttg tcctgccggc gagaaacgct gtggagcggc
     4501 cggcctggcc cgaacctgag ttcgacgaca gcttcaccgg agaggatagg cgtcgatgac
     4561 cgttcgcgtg ctcctggcag aggaccaggc gatggtgcgt ggcgcgctct ccgccctgct
     4621 cactctggag gatgacatcg agatcgtggc cgaggcatcg cgcggggacg aggtacttcc
     4681 cgccgccctg gataccctgc ccgacgtggc gctgctcgac atagagatgc cgggcgggga
     4741 cggtctcgag gccgcggccg tcctgcatga gcggctgcct tcgtgcctgg tggtcatcct
     4801 gacgaccttc gggcgctcag ggtacctcag gcgggccatg gagagcggcg cggtcgggtt
     4861 cttactcaag gacgcccctg cctctgaact ggccgaggcc atccgcaggg ccgtgaaagg
     4921 cgagcgggtg gtagaccccg ccctggcggc cgccgccctc agcgagggcg acaaccctct
     4981 caccgaacgc gagcgcgaag tgctcgcagc ctcagaaggc ggagcaacga tagaagatat
     5041 agcggccagg ctctacctct ccgaaggcac cgtgcgcaac tacctctcca cggccatcaa
     5101 gaaactcggg gcgcgaaacc gcgtcgaggc agcgcgtctg gccgagaaga aaggctggtt
     5161 gtagccgtca gttcttcctg atgcctgaaa cctatttctc cttcacgtac gtgcctgggg
     5221 catcctctat gggcgggtag ttctcgctgc ccgtatccgg gggggtcacc atcgctcctg
     5281 agtgggcgtc gagccacttg ctccagtctt cccaccagga tccttcgtgc tccctggcgt
     5341 tctcgcgcca gtctgcgggg gcatcgaagg cgccgccgtt ctcggtcgcc cagtagcggc
     5401 ccttgccctt ggatgggggg ttgatcatgc ccgctatgtg gcccgagttg gctaaggtga
     5461 agcgggtggt gccgtttgcg agcctggaga tcttccacgc cgaggcccag gggacgatgt
     5521 gatctttctc ggcgccgacg gcgtagatgt cgccctcgat cctggagagg tctatggggc
     5581 ggccctttat ctctaccttt cctggctcta tgaggttgtt ctcgaggtag gtattgcgca
     5641 ggtagaagct gtgggcgtcg cgtgccatgc gcgtcccgtc cgagttccag taaaggaggt
     5701 cgaaggcagg aggcttctgc ccgaggaggt aattgttgac gacgttggac cagatcaggt
     5761 cgttggagcg gagcaagttg aacatgttgg ccatcttgcg gttgtcgaga tagccgcgct
     5821 cgagcatctg ctgctccatg aactctatct gcggctcgtc tatgaagacc cctgtgtccc
     5881 ccacctccgt g
//
LOCUS       DATMJA010000371         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_465011, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000371 DATMJA010000000
VERSION     DATMJA010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465011"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1034
                     /locus_tag="VNT12_08925"
     CDS             51..1034
                     /locus_tag="VNT12_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI46281.1"
                     /translation="MRDPGNRFRFELAGSQWSENRGEWRMPVMITESTSIDYDINGDG
                     PPLVLISGLGFGRWGWFKQVPALSRHFRTITFDIRGERTLENGVHDLVRDVVSLLDHL
                     GVKKAHVFGASLGGFVGQELALLRPDLVDRLVLVSTSYGGRGPESMSMQALSDMLGWG
                     SLSPEGVVRRGLETATSGTYRAKRGEEFEQIVSWRLADSPSSAAYYEQARAGAGFDLS
                     RGVEHITSPTLVIHGAEDRYVPVANAAALAGAIPGARLRVLDRAGHLVFIERFADVNR
                     GVVGFLKPRGSRRQAGANVQRARRRDRVLGWIREVPRSLWRWSGRLWDSIF"
     gene            1146..>2524
                     /locus_tag="VNT12_08930"
     CDS             1146..>2524
                     /locus_tag="VNT12_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HWI46282.1"
                     /translation="MSEGKTGNRSSDVTPEMAPFDVWSEWMRANMGPMTATPGASVPW
                     LMMPGVRTGEEVAELPEGAMRNDPLLSTVEKLWDANPMQSVLPIDWVEITRALQTLWA
                     REMSDPARALERATDFNARLFGATMRSWQEATNRLWGLQTSQEEDEGRPDKRFSAPDW
                     EQNPFYETLKESYLLASEYLLDEAEKTDGEDTPEQRRLKFHLKQFVDAMAPVNYLLTN
                     PAALKRAFETGGASLAEGARNLLADLQEGRMSMTDANAFQPGENLAITPGKVVYRNEL
                     IELIQYEPTTDKVHETPIMFIPPWINKYYILDLQPKNSFVKYLVDEGYTVFMISWKNP
                     DASMEDTKFEDYMTKGPLAAAEVVRDITGSEKVNPVGYCVGGTLLAIALSWIAAGEDE
                     NPFGPPTFMVALHDFTEVGDTGVFIDEPQIEFMEQQMLERGYLDNRKMANMFNLLRSN
                     DLIWSNVVN"
BASE COUNT          499 a          746 c          826 g          453 t
ORIGIN      
        1 gtgttgcatc taagtgcaga gtgcatttat cctattagaa ggagatgctg gtgcgggatc
       61 cgggcaaccg gttccgcttc gagctggccg gctctcagtg gagcgagaat cgaggtgagt
      121 ggcgaatgcc ggtgatgatc accgagagca cgagcatcga ctacgacata aatggggatg
      181 ggccgcctct tgtgctcata agcggccttg gcttcggccg ctggggttgg ttcaagcagg
      241 tccccgctct ctcgcgccac ttccggacca taaccttcga catcaggggt gagcgcacgc
      301 tcgagaacgg cgtccatgac ctggtccgtg acgtggtgtc gctgctggac cacctgggtg
      361 taaagaaggc ccatgtcttc ggagcttcgc tcggcggctt tgtggggcag gagctggcgt
      421 tgctcaggcc agatctggtc gaccgtctag tgctggtctc caccagctac ggtggcaggg
      481 gaccggagtc catgtctatg caggcccttt cggacatgct tggatggggt tccctttctc
      541 cagagggagt cgtcagacgg ggtctcgaga cggccacctc ggggacctat cgcgccaagc
      601 gcggtgaaga gttcgagcag atcgtaagct ggcgtctcgc tgactcgccc tccagcgctg
      661 cctactacga gcaggccagg gcaggggcgg gcttcgacct ctcccggggt gttgagcaca
      721 taacctcacc cacgcttgtg atccacggtg ctgaggaccg ctacgtgccc gtcgccaacg
      781 cggcggccct cgcaggggcc atcccaggcg ccaggctacg ggtcctcgac cgcgcgggcc
      841 acctcgtctt tatcgagcgg ttcgccgacg tgaacagagg ggtggtcggc ttcctcaagc
      901 cgcgcgggtc ccgcaggcag gccggcgcca acgtacagcg ggcgaggcgg cgggacaggg
      961 tactgggttg gatcagggag gtgccgcggt cgctctggcg ctggtcgggg aggctgtggg
     1021 acagcatctt ctgagttacc ttgcaggccg tttttgggcg acgggcacac tgggtagaat
     1081 agtacggccg aatacaagct ggcggcgctg gccgcccggt gaatctctct gaggaggaag
     1141 actagatgag cgaaggcaag accggaaacc gcagttccga tgttacgccc gagatggcgc
     1201 cattcgacgt atggtcggag tggatgcggg cgaacatggg gcctatgacg gcgacgccgg
     1261 gcgcgagcgt accctggctc atgatgcctg gtgtcaggac aggagaagag gtcgcggagt
     1321 tgcccgaggg ggctatgcgg aacgatcctt tgctctctac cgtcgagaag ttgtgggacg
     1381 ccaaccctat gcagagcgtt ttgcccattg actgggtcga gatcacacgc gcgctgcaga
     1441 ccctctgggc gcgcgagatg tccgaccccg cgcgcgccct ggagcgggct acggacttca
     1501 acgccaggct gttcggcgcc acgatgagga gctggcagga ggccacaaac cgtctctggg
     1561 gcctccagac atcgcaagag gaagacgagg gccgtcccga caagcggttc tcggcgccag
     1621 actgggagca gaacccattc tacgagaccc tcaaggagtc atacctgctc gcctcggaat
     1681 atctgctcga tgaggcggag aagaccgatg gggaggacac tcccgagcag cggcgtctaa
     1741 agttccacct caagcagttc gtagacgcca tggctcctgt caactatctg ctgaccaacc
     1801 ctgcggcgct caagagggcc ttcgagaccg ggggagccag cctcgccgaa ggcgcccgca
     1861 acctgctcgc ggacctgcaa gaaggccgca tgagcatgac cgacgccaac gccttccagc
     1921 cgggagagaa cctggccatc acaccgggca aggtcgtcta ccgcaacgag ctcatagagt
     1981 tgatccagta cgagccgacc acagacaagg ttcacgagac gcctatcatg ttcataccgc
     2041 cttggattaa caagtactac atcctcgatt tgcagccgaa gaacagcttc gtcaagtacc
     2101 tggtggacga gggctacacg gtgttcatga tctcctggaa gaacccggac gcctcgatgg
     2161 aggacaccaa attcgaggac tacatgacaa agggaccgct cgccgcggcc gaggtcgtga
     2221 gggacatcac gggttcagag aaggtcaacc ccgtcggcta ttgcgtgggg ggtactctgc
     2281 tcgcgatcgc gctctcctgg atcgccgccg gtgaagacga gaaccccttc gggccgccta
     2341 ccttcatggt cgcgttgcac gacttcaccg aggtggggga cacaggggtc tttatagacg
     2401 agccgcagat agagttcatg gagcagcaga tgctcgagcg tggctacctc gacaaccgca
     2461 agatggccaa catgttcaac ttgctccgct ccaacgacct gatctggtcc aacgtcgtca
     2521 acaa
//
LOCUS       DATMJA010000372         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_228379, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000372 DATMJA010000000
VERSION     DATMJA010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228379"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VNT12_08935"
     CDS             complement(<1..543)
                     /locus_tag="VNT12_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HWI46283.1"
                     /translation="MIDLRSDTVTRPTEGMRRAMLEAPVGDDVFGEDPTVNRLEEYVA
                     DLLGKEAALYAPSGTMTNQVGVQVNTNRGEEVLLHEGSHIFVYEAGAPAMLSGVQLRT
                     LPGKNGVIDPETVRAAVRPEDVHFPRSRLLCLENTHNTSGGKVFPLEDFAAVATTAKE
                     LDLKVHLDGARLFNAQAATGV"
     gene            complement(540..1478)
                     /locus_tag="VNT12_08940"
     CDS             complement(540..1478)
                     /locus_tag="VNT12_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46284.1"
                     /translation="MNPPTEVPPDLRSLLDREGVEIAGSRAIDHGTQYDLARDEETAK
                     LNVYRTGKISAGGKASRLRELLEGWRTSQTSVGGTRASTDRAGRRPLLDGTPRLGIDE
                     AGKGDYFGPLVVAGVRITHAVTASKLQEIGVRDSKEIRGPGISRISGRILEAVGRNNV
                     HVISLPPPEYESRRAAAGNVNRLLSELDATIMGKLVDKVEIVVVDEFARSARSYLEPF
                     VPDGVRLEVRTRAEDDAAVAAASIVARARQLEEIERLSERVGFGLPLGATHVVEAGRR
                     VVGELGEDGLAEVAKIHFATTRRVLETAEQSDGGDL"
     gene            complement(1475..1759)
                     /locus_tag="VNT12_08945"
     CDS             complement(1475..1759)
                     /locus_tag="VNT12_08945"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWI46285.1"
                     /translation="MQNSDLQRAHVRVSGQVQGVFFRDSTRQKAKELGLAGWVRNVPD
                     GQVEALFEGPSKKVEEMVRWCEEGPQRASVENVDTDFESAGGDLEGFEVR"
     gene            1845..>2695
                     /locus_tag="VNT12_08950"
     CDS             1845..>2695
                     /locus_tag="VNT12_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWI46286.1"
                     /translation="MKADKQAVTGGPERGRSQEKELLTLVVPTRNEAENIPKLVHELR
                     ESLLDLDYKVVFVDDSTDETPEVIQGLSEQDGRVQLVRREGTERYGGLSTAVTTGMEL
                     FSGASEYTCVMDADLQHPPEKVREMLEIARRSDADVVVASRYGPGGSYAGLSGRTRRA
                     VSVGSKYLARILFKEARKTNDPMAGFFLIKNQAISGIQFRPTGFKVLLEILVCAPELK
                     VLETPYAFQVRHAGVSKATMRQGFEYLTHILSLFWYVPSAGRIWKFLLVGASGVLVNM
                     VTLIVLA"
BASE COUNT          461 a          892 c          813 g          529 t
ORIGIN      
        1 gacgccggtc gcagcctggg cgttgaagag cctggcaccg tcgaggtgaa ccttcaggtc
       61 aagctctttc gccgttgtgg cgaccgcggc gaagtcctcg agcgggaaga ccttgccgcc
      121 tgaggtattg tgggtgttct ccaggcagag cagcctggag cgcgggaagt ggacgtcctc
      181 gggcctgaca gcagcgcgga cggtctccgg gtcgatgacg ccgttcttcc cggggagggt
      241 gcggagctgg acaccggaga gcatcgccgg cgctccggcc tcgtagacga agatgtggga
      301 gccttcgtgc aggaggacct cttctccgcg gttcgtgttg acctggaccc cgacctggtt
      361 ggtcatcgtc cctgaaggcg cgtacagcgc cgcctccttg cccagcaggt ccgccacgta
      421 ctcctcgagc ctgttcaccg tcgggtcctc accgaacacg tcgtctccaa ccggcgcctc
      481 gagcatcgcc cgccgcatac cctccgtagg acgggtcacc gtgtcgctgc gaagatctat
      541 cacaggtctc ctccatcact ctgttcggcc gtctccagca cccgacgcgt ggtggcgaag
      601 tggatcttcg ccacctccgc cagaccgtct tccccgagtt ccccgaccac ccgccgaccg
      661 gcctccacaa cgtgcgtcgc tcctagtggc aatccgaacc ccacccgctc cgagagacgc
      721 tcgatctcct cgagttgccg cgcccgcgcc actatcgacg cagcagccac ggctgcatcg
      781 tcctcagccc gcgtgcgtac ctccagccgc acgccgtcag gcacgaacgg ctcgagatag
      841 gagcgggcgg atctggcaaa ctcgtccacc actactattt caactttgtc cacaagttta
      901 cccatgatcg tggcgtcgag ctcgctgagc agcctgttga cgtttccggc ggcggcccgg
      961 cgggactcgt actccggagg cgggagcgat atgacgtgga cgttgttccg gcctacggct
     1021 tccaggatgc gccctgagat ccgggagatg cctgggcccc tgatctcctt tgaatccctg
     1081 acccctatct cctgcagctt actcgccgtc actgcgtgtg ttatccggac cccggcgacg
     1141 accagaggac cgaagtagtc gcccttcccg gcctcgtcga tgccgagcct cggcgtaccg
     1201 tcgagaaggg gtctccttcc cgcgcggtcc gtcgaagccc gcgtaccacc aacactggtc
     1261 tgcgacgtgc gccagccttc gagcagctcc cgcaaccttg aagctttacc tcccgcagag
     1321 atcttgcccg tgcgatagac gttcagcttc gcggtctcct cgtcgcgggc caggtcgtac
     1381 tgagtcccgt gatctatcgc tctgctaccc gctatctcaa caccttctct gtcgaggagc
     1441 gagcgcaggt ctggagggac ttcggtgggt gggttcaacg gacctcgaag ccttcgagat
     1501 ctccgcctgc gctctcgaag tccgtgtcca cgttctccac cgaggcgcgc tgagggcctt
     1561 cttcgcacca gcgaaccatc tcttcgacct ttttagaagg gccttcgaac agggcttcga
     1621 cctgcccgtc aggcacattc ctcacccatc ccgccaggcc gagttctttg gccttctgcc
     1681 tggtcgagtc cctgaagaag accccctgca cctggcctga gacccggacg tgcgcgcgct
     1741 gtagatcgct gttttgcact ttgcgtacct ccgtggggag cctttacggt cagtttatcc
     1801 cctattttgg gcaggttaat ccgatatgct agcctagagc ggccatgaaa gcagacaagc
     1861 aagcggttac cggcgggccg gaacgcggga ggagccagga gaaggaactc cttaccctgg
     1921 tcgtgccgac acggaacgag gccgagaaca tcccgaagct tgtgcacgag ttgcgcgaga
     1981 gccttttgga cctggattac aaggtggtct tcgtggatga ctcgacggac gaaacgccgg
     2041 aggtgataca gggcctctca gaacaggacg gacgcgtcca gctggtccgc cgagaaggga
     2101 cagagcggta cggtggcctc tcgacggctg tgacgaccgg tatggagctg ttctctggcg
     2161 cgagcgagta cacctgtgtg atggacgcgg acttacaaca ccctcccgag aaagtgcgtg
     2221 agatgctgga gatcgcacgc cgctccgacg ccgacgtcgt tgtggcgagt cgctacggcc
     2281 cgggcggcag ctacgcaggc ctctctggca gaacgcggcg ggcagtgtcc gtgggaagca
     2341 aatacctggc ccggatcctc ttcaaggagg cccgcaagac gaatgatccg atggcgggct
     2401 tcttcctcat aaagaaccag gcgatctcag gcatccagtt caggcctacg gggttcaagg
     2461 tgctgctcga gattctcgtg tgcgcacccg agctcaaggt actcgaaacg ccttacgcgt
     2521 tccaggttcg ccatgccggg gtatccaagg cgaccatgcg ccagggcttc gagtacctga
     2581 ctcacatact gagccttttc tggtacgttc cttcggcggg gcgcatctgg aagttcctcc
     2641 tcgtcggggc ctccggcgtg ctggtcaaca tggttaccct catcgtgctc gccga
//
LOCUS       DATMJA010000373         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_169465, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000373 DATMJA010000000
VERSION     DATMJA010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169465"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..366
                     /locus_tag="VNT12_08955"
     CDS             91..366
                     /locus_tag="VNT12_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWI46287.1"
                     /translation="MRKLCQIFVCATPGEGRCGAKGGGELFEMFKEEVELRGIPPSAV
                     LRNACTRRHEEGPVVFVYPDDVWYTCVTPEDVPEIVDRHLAPIRVET"
     gene            complement(390..1658)
                     /locus_tag="VNT12_08960"
     CDS             complement(390..1658)
                     /locus_tag="VNT12_08960"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI46288.1"
                     /translation="MARNVRRSAQVVVLGAGFAGASLLRHLSPVLRRPGKTLLIDRRE
                     EYTFVPLIHEVAVGRVHPRTVSSPIAPLCAGVCEFLHSEVSGIDLAKKTLETSEGPVG
                     YEYLVLNPGSVAAEPGPRLQDYFHSFWSLDDALRLRTALGDAWRRATSPGPRAPQGIL
                     TVAIVGGGATGVEVAAEVAALFDYLRKRSHRRPSEEPRVVLLEATHRLMDWLDPYFDS
                     VARKTLSGLGVEVRLNTPVETASAEGVLAGENWLKAATRVWTAGVEASPLARDLPGEH
                     DAAGRSHVGEHLTLADHPEVYVLGDAGVYTDPRLGPLPPTASVAVQQGPWVARDLKRR
                     LRGAESKRGRPPFRFFDRGYVVSLGPESAVADAVGLRLRGPAAQALYRSVLLYYMKSR
                     RDRLLTGADWAMERTLGRVGFGSDRPSAKT"
     gene            1875..2201
                     /locus_tag="VNT12_08965"
     CDS             1875..2201
                     /locus_tag="VNT12_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46289.1"
                     /translation="MVMKEGMGLGDIAPGLIVRHTNRRLGKLDPYEACERLISGRISF
                     SEFVDQVGEEYRELLDFMMDNDLHPGRPEEVLFCIDCHAGLCDRTLLRSQLECLYSHD
                     RRERIG"
     gene            2364..>2695
                     /locus_tag="VNT12_08970"
     CDS             2364..>2695
                     /locus_tag="VNT12_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI46290.1"
                     /translation="MGITLIVLFVVVAVILAASIVLGRDAREVESARDTEYLELEGVW
                     IRYDVIGAGPAVVLVHGWLSSSRIWEQLAARLAQRFTVYTLDLSGFGESDKPLSGYGI
                     RNGSRLLYA"
BASE COUNT          461 a          838 c          909 g          487 t
ORIGIN      
        1 tcaggagcgc gccgaaaccg gactgacggc gaccagcttc tagaggctac agcgtctgga
       61 cttctcctgg ctgcggcata gaatctgggc atgaggaagc tgtgccagat cttcgtttgc
      121 gccacgccgg gtgagggacg ctgcggcgcg aaggggggcg gcgaactgtt cgagatgttc
      181 aaggaagagg tggagctgcg cggcataccg ccgtccgccg tgctccgtaa cgcatgtacc
      241 cgccgccacg aggaaggtcc cgtcgtgttc gtctaccctg acgacgtctg gtatacctgc
      301 gtaacacccg aagacgtgcc agaaatagtg gatcgtcacc tggcgccaat tcgggtcgag
      361 acgtagagca gcacttcaac tggtcagctt catgttttgg ctgacggccg atcgctcccg
      421 aagccgaccc ttccgagggt ccgctccatg gcccagtctg cgccggtgag tagcctgtcg
      481 cgccggctct tcatgtagta gaggaggacg ctcctgtaga gcgcctgtgc ggcggggcct
      541 cgcagcctga ggccgacggc gtcggccacg gcgctctctg ggccgaggct cacgacgtag
      601 ccccggtcga agaagcggaa aggtggcctc ccgcgcttgc tctcagcgcc acgaaggcgg
      661 cgcttgaggt cgcgtgccac ccaaggcccc tgctgcacgg cgacggaggc ggtggggggc
      721 aggggaccga ggcgcggatc ggtgtatacc ccggcgtcac cgagcacgta gacctcggga
      781 tgatccgcca gggtgaggtg ctcgccaaca tgcgagcggc ccgccgcatc gtgctcccca
      841 ggcaggtccc tcgccaaggg cgaggcctca acgccggccg tccataccct ggtggccgcc
      901 ttcagccagt tttcgccggc gaggacgccc tcggcgctcg ccgtctctac gggggtgttc
      961 aggcgcactt cgacgccgag gccagagagc gttttcctcg cgacgctgtc gaagtagggg
     1021 tctagccaat ccatcagccg gtgcgtagcc tcgagcagca ctacccgcgg ttcttcggac
     1081 ggcctgcgat gggagcgttt ccggagatag tcgaagaggg cggccacctc ggcggcgacc
     1141 tccacgcctg tcgccccccc gccgacgatg gccacggtca ggatgccctg cggcgcccgg
     1201 ggccccgggc tcgtggcacg gcgccaggca tcgccaagtg cggtgcgcag gcgcagcgca
     1261 tcgtccaggc tccagaagga atggaagtaa tcctgcagcc gcggccccgg ctcggctgcg
     1321 acactgccag ggttgagtac cagatactcg tagccgacgg gcccttcgga ggtttcgagg
     1381 gtcttcttgg cgaggtcgat acccgagact tccgagtgca ggaactcgca gaccccggcg
     1441 cagagcgggg cgatgggtga gcttaccgtc ctcggatgca cgcggccgac tgcgacctcg
     1501 tggatcaggg gcacgaaagt gtactcttcc cggcggtcga tcagcagggt cttgccgggt
     1561 cttcgcagga ccggagagag atgcctgagt agagaggcgc ctgcgaaccc tgcgcctaga
     1621 acgaccacct gcgcactgcg tcggacgtta cgggccatac cccagaatgt ccttccggca
     1681 cgtccttgtt tccttcgcta caccaggcat cttccgggag tatatcgttc ggggtgcccc
     1741 ctctcggcga gacgctttac aaaacgcgcg cctgaacgta gactgcacgg atcgcctgct
     1801 gatggggtgc cggcggtcta aggggtgtca ggaaaacggg gaacgacccc tccgggggct
     1861 agaaagggac cgtcttggtg atgaaggagg gcatgggatt gggggacatc gcccctgggc
     1921 tcatcgtgag gcacaccaac cgtcgtctgg gcaagctgga tccctacgaa gcgtgcgagc
     1981 gtttgatctc tggccgcatc tcgttctcgg agttcgtgga tcaggtcggc gaagagtaca
     2041 gggagctcct cgatttcatg atggacaacg acctgcaccc tggccgtcct gaggaggtgc
     2101 tcttctgcat cgactgtcat gcaggtctct gcgatcgtac tctccttcgc tcgcaactgg
     2161 aatgcctcta cagtcacgac aggcgcgaaa ggatcggctg accggaaatg cgacgtcaca
     2221 ggccacgtga ggcctggctc tgcggcaccg attcgcggtt cgtgaggtca aagtagggag
     2281 agggccaacc ccgcgaaggc aggacgagta gggcgcgtcg tgcctgtatc ctgttggcgc
     2341 gtttttgcgc tatcatggcc tctatgggca tcacattgat agtcttgttc gttgtggttg
     2401 ctgtgatcct cgccgcctcc atcgtgctcg ggcgtgacgc cagggaggtg gagagcgccc
     2461 gtgacacgga gtacctggag ctcgaagggg tctggatccg ctacgacgtc atcggcgcgg
     2521 gacccgcggt cgtactcgtg cacggctggt tatcctcgtc ccgcatctgg gagcaactcg
     2581 cagcgcggct ggcgcagcgc ttcaccgtgt ataccctcga cctgagcggt ttcggagagt
     2641 cggacaagcc cctctccggc tatgggatac gaaacgggag caggcttctc tacgc
//
LOCUS       DATMJA010000374         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_228740, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000374 DATMJA010000000
VERSION     DATMJA010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228740"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2750
                     /gene="treS"
                     /locus_tag="VNT12_08975"
     CDS             <1..>2750
                     /gene="treS"
                     /locus_tag="VNT12_08975"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWI46291.1"
                     /translation="DFRAFVRKAHELGLKVITELVINHTSDEHAWFQRARKAPKDSAK
                     RNFYVWSDNQDKYEGTRIIFTDTERSNWTWDEEAGQYYWHRFFSHQPDLNHDNPQVFK
                     AIMRVMRFWLDAGVDGLRLDAIPYLIEREGTDNENLPETHEVLKQMRAEMDARYGNRL
                     FLAEANQWPEDVLPYFGEGDECHMAFHFPLMPRIFMALAQEDRHPIVEIMHQTPEIPE
                     SCQWAIFLRNHDELTLEMVTDRERDYMYQIYATDPRMRVNVGIRRRLASMMENDRPRI
                     ELLNSLLMSMPGTPIIYYGDEIGMGDNIFLGDRDAVRTPMQWTSDRNAGFSRTDPARL
                     YLPPIMDPVYGYESVNVDAQSRSAGSLLNWMKRIISVRKAHRAFGRGTLEFLHPGNRK
                     VIAYLRAHDDESILCVANFSRSAQPVELDLARFKGRVPVELLGNSSFPPLGDLPYFLT
                     LPGHGFYWFRLAKEAVAPRWHQESPRYAEPPVLVLPAGRETAHRVYSTPLRLPSRQLA
                     QLEREALPNFLGTRRWFAGKGGEIERVEVVAQAELADKGLLTLLRVHVDDESPEETGP
                     QLYFLPLAAAWEGGTEEDGVSVLMPHALAKLRRRARMGLLFDAMADEQFCRHVVETMG
                     KDREVGIGQGRIRFSGTDVFGELIGEESVSELSVRRAEVEQTNSSVILGERLILKAYR
                     RLQKGTNPDLEIGRYLTEEVRFENTPPLAGAIEYEGPDGETTTLGLLQGFVENQGDGW
                     SYVLDYLGRYLEDRLVTSWSPEGEEDVEAEREERGAEDAFFASLVRILGQRTGELHAA
                     FAAPTDDSAFAPEHASPEEVAGWAEDILAEARRTLDLLKRRRGRLPEEDGPDADRLLT
                     LRRELTRRIKDVSREGFGVVKTRHHGDYHLGQVLVVGNDFQIIDFEGEPARPLAERRR
                     KHS"
BASE COUNT          537 a          877 c          891 g          445 t
ORIGIN      
        1 gacttcaggg ccttcgtgcg caaggcgcac gagcttggac tgaaggtcat aaccgagctg
       61 gtcataaacc acacctccga tgagcacgcg tggttccagc gcgcccggaa ggcaccgaag
      121 gactctgcga agcgcaactt ctacgtctgg agcgacaacc aggacaagta cgagggcacc
      181 cgcatcatct tcaccgacac agagcgctcc aactggacct gggacgagga ggccggccaa
      241 tactactggc accgtttctt ctcccaccag cctgacctca accacgacaa cccgcaggtc
      301 ttcaaggcga taatgcgcgt gatgcgcttc tggctcgacg caggcgttga cggcctcagg
      361 ctcgatgcga tcccttacct gatcgaacgc gagggcacgg acaacgagaa cctccccgag
      421 acgcacgagg tcctcaagca gatgcgcgcc gagatggacg cccgctacgg gaaccgcctc
      481 ttcctcgccg aggcgaacca gtggccggag gacgtcctgc catacttcgg ggagggtgac
      541 gagtgccaca tggccttcca cttcccgctc atgccccgca tcttcatggc gctcgcgcag
      601 gaagataggc accccatcgt ggagatcatg caccagacgc ccgagatccc cgagagctgt
      661 cagtgggcga tcttcctccg aaaccacgac gagcttaccc tcgagatggt cacggacagg
      721 gagcgcgact acatgtacca gatctacgct accgacccgc ggatgcgcgt gaacgtgggc
      781 atcagacgtc gtctcgcctc catgatggag aacgacagac cccgcatcga gctcctgaac
      841 agcctgctca tgagcatgcc aggcacgccc atcatctact acggggacga gatcgggatg
      901 ggcgacaata tcttcctcgg cgaccgggac gccgtaagga cgcccatgca gtggacgagc
      961 gataggaacg ctggcttctc ccggacggat ccagcccgcc tgtacctgcc gcccatcatg
     1021 gaccctgtct acggctacga gtcggtgaac gtcgatgccc agagccgcag tgcgggctcg
     1081 ctcctgaact ggatgaagcg catcatctcg gtccgcaagg cgcacagggc cttcggacgt
     1141 ggaaccctgg agtttttgca ccccggcaac aggaaggtca tcgcctactt gagggcacac
     1201 gacgacgaat ccatcctttg cgtggcgaac ttcagcaggt cggcccagcc tgtggaactc
     1261 gacctcgcgc gcttcaaggg ccgggttccg gtcgagttgc tcggcaacag cagcttccct
     1321 cctctgggag acctgccata tttcctcacc ctgccggggc acggctttta ctggttccgc
     1381 cttgcaaaag aggccgtggc cccgagatgg caccaggaga gcccgcggta cgccgagccg
     1441 cccgttctgg ttcttcccgc cggacgggag acagcccaca gggtctacag tacccccctg
     1501 cgtctcccga gccgtcagct cgcgcaactg gagagggagg cgctgccgaa ctttctcggc
     1561 acccgtcggt ggttcgcggg gaagggtggt gagatcgagc gcgtcgaggt cgtagcgcag
     1621 gccgagctcg ccgacaaagg cttactgacc ctgcttcggg tgcacgtgga cgacgagtct
     1681 cctgaagaga ccgggcctca gctgtacttc ctcccgctcg ccgcggcctg ggaggggggc
     1741 accgaggagg acggcgtctc ggtgttaatg ccccacgctc tggcgaagct ccggcgccgg
     1801 gccaggatgg gcctcctctt cgacgccatg gctgacgagc agttctgccg tcacgtggtt
     1861 gagacgatgg gtaaggatag ggaggtgggc attggacagg gcaggatccg cttctcaggc
     1921 acggatgtct tcggtgagct tatcggggag gagtctgtct ccgaactgag cgtgcggcgc
     1981 gccgaggtgg agcagaccaa tagctccgtg atactcggcg agaggctcat cctgaaggca
     2041 taccggcgcc tccagaaagg gacgaatccg gacctggaga tcggacgcta cctgaccgag
     2101 gaggtacgct tcgagaacac gccgccgctg gccggggcca tcgaatacga agggcccgac
     2161 ggcgaaacga ccaccctcgg cctcctgcag ggcttcgtgg agaaccaggg tgacggatgg
     2221 tcctacgttc tcgactacct cggccgctat ctggaagacc gcctggtgac ctcgtggagc
     2281 cctgagggcg aggaggacgt cgaggcggag cgggaggagc ggggtgctga ggacgccttc
     2341 ttcgcgagtc tggtacgcat cctggggcag cgtacaggtg agctgcacgc ggctttcgcc
     2401 gcgcccacgg acgactcggc cttcgccccc gagcacgctt ctccagagga ggtggccggc
     2461 tgggccgagg acatactcgc ggaggccagg cgcacgttgg atctactgaa gagacgcagg
     2521 ggcaggttgc cagaggagga cggccccgac gcggaccgcc ttctgacgct gcgcagggag
     2581 ttgacgaggc gcataaagga cgtctccaga gaaggtttcg gggtcgtaaa gaccaggcac
     2641 cacggcgact accacctcgg gcaggtgctc gtggtcggca acgacttcca gatcatagat
     2701 ttcgagggcg agccggccag gcccctcgcc gagcggcgca ggaagcactc
//
LOCUS       DATMJA010000375         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_821136, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000375 DATMJA010000000
VERSION     DATMJA010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821136"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VNT12_08980"
     CDS             <1..289
                     /locus_tag="VNT12_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46292.1"
                     /translation="PLPLSFVLAGLYHLGALGGRHGYVVTWRILAYPLGFYLPFAAVP
                     LLGPLGAAYSGLVLMGLGLAEVQEIGRLWSGLFCTSVVAVCGGAYYFLSVA"
     gene            complement(302..778)
                     /locus_tag="VNT12_08985"
     CDS             complement(302..778)
                     /locus_tag="VNT12_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI46293.1"
                     /translation="MGFNYKVREASVEDAAEICGLARELAETVGDEPPTEKATRARLE
                     ELLDEPRARVFVAENEAGIVGGVSFWIKPDLAHGDTVVEVPMLVVAEDHRRTGVGKLL
                     MEEVRNVASDNGASQIELVATRSNVTAREFYRSLGFVESDVVSLEFVGSQEDPPDE"
     gene            865..2073
                     /locus_tag="VNT12_08990"
     CDS             865..2073
                     /locus_tag="VNT12_08990"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HWI46294.1"
                     /translation="MERRRMGRRVVVIVLDGLGAGKAPDSADFGDEGANTLANTAGST
                     GGLRTPNLQALGLGNIEEIEGIAPSASPAASHGLMVELSAAKATLAGHWEMMGLVLED
                     PLPTYPEGFPDRVLRRFEKVTGRGVLGNRPASGTRIIEELGPEQERTGAWILYTSADS
                     VFQIAANTGVIPLDELYEASEKAHKMLIDAGEIMVERVIARPFHGEPGAYVRENENRH
                     DYGITPPSETYLDRIKDAGHETVAVGKIKDIFDGRGITEHLPAPPDDAAKVDAILDAL
                     GHVESGLIFANLVDFDAKYGHRRDPEGMAENIQRFDERLPELLDALTEQDLLIITADH
                     GNDPTFRGTDHTRERVPLLVIGSGEPHALGIREGFSDVGASVATWLRVEQGGLPGRSF
                     VQGAGDSSTR"
     gene            2113..>2601
                     /locus_tag="VNT12_08995"
     CDS             2113..>2601
                     /locus_tag="VNT12_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46295.1"
                     /translation="MGSGNAILDAIEAKKFGGELSDGLIRDVVGGYVMGEVPDYQMAS
                     LLMAIFIQGMSYGETLALTRAMAESGETYSFPECVDKHSTGGVGDKVSLTALPIVAAC
                     GAPVAKLSGRGLGSTGGTIDKLESIPGFSCNLSEEQFRNQVEEVGLAIIEAADLAPAD
                     KEI"
BASE COUNT          481 a          820 c          834 g          466 t
ORIGIN      
        1 gccgctgccg ctatccttcg tcctcgccgg cctctaccat ctcggcgctc tcggtggccg
       61 gcacgggtac gtggtgacct ggaggattct cgcgtacccg ctcggtttct acctgccctt
      121 cgccgcagta cctttgctcg ggcccctggg agccgcttac tcggggctcg tgctcatggg
      181 gctagggctc gctgaggtgc aggagatcgg aaggctctgg tccgggctct tctgtacgtc
      241 tgttgtagcc gtatgcgggg gggcttacta cttcctgagc gttgcctgat ccgggcgtgg
      301 gtcattcgtc gggcgggtct tcctgagatc ctacaaactc cagcgagact acgtcggact
      361 cgacgaagcc gagggagcgg tagaactcgc gggccgttac gttcgatcgg gtggcgacca
      421 gttcgatctg tgatgctccg ttgtcggaag ctacgttgcg cacctcctcc atgaggagct
      481 tgcccacccc agtccgcctg tggtcctccg cgacgacgag catcggcacc tcgacgacgg
      541 tgtcgccgtg ggcgaggtcg ggtttgatcc agaagctcac cccgcccacg atcccggcct
      601 cgttctcggc gacgaagacc cgtgccctgg gttcgtccag gagctcctcg aggcgcgccc
      661 tggtggcctt ctctgttggc ggttcgtcgc cgaccgtctc ggccagctcg cgggcgagac
      721 cgcagatctc cgcggcgtcc tcgacgcttg cctctcgaac cttgtaattg aagcccatgc
      781 tactcctcat ctcgtagtct ggagatatta cttaccctac ggagcgcggc gacgaacgga
      841 tgaaggcgct aagatgatcc agacgtggaa aggagacgca tgggacgcag ggtggtcgtg
      901 atcgtgctcg acggactcgg cgcgggcaaa gctcctgatt cggcggactt cggcgacgaa
      961 ggcgccaaca ccctggcgaa cacggcagga tccacaggcg gcctccgaac gccgaacctc
     1021 caggccctcg ggctcggtaa catcgaggag atagaaggca tagctccctc cgcctctcct
     1081 gcggcttccc acggcctgat ggtcgagtta tcagcggcca aggccaccct cgcaggacac
     1141 tgggagatga tgggcctcgt acttgaagac ccgctcccta cctatccaga aggctttccc
     1201 gacagggtgt tgcgtcgctt cgaaaaggtg acggggcgcg gggtattggg gaacaggcca
     1261 gcctcgggga ccaggatcat cgaggagctg ggtcccgagc aggagaggac aggggcctgg
     1321 atcctctata cctccgctga ctccgtcttc cagatcgcgg caaacaccgg cgtgatcccg
     1381 ctcgatgagc tgtatgaggc ttccgaaaag gcacacaaga tgctgatcga cgcgggtgag
     1441 atcatggtcg aacgggtcat cgcccgtccc ttccacggcg agcccggcgc ctacgtccgc
     1501 gaaaacgaga accgccacga ctacggcata accccgccct cggagaccta tctggaccgc
     1561 ataaaggacg caggacacga gaccgtggcc gtaggcaaga tcaaggacat cttcgacggc
     1621 cgtggcataa ctgagcactt gcccgccccg cccgacgatg cggccaaagt agacgcgatt
     1681 ctggatgcgc tgggccatgt cgagtcgggc ttgatcttcg caaacctcgt tgacttcgac
     1741 gccaaatacg gccaccgtcg cgaccccgag ggtatggccg aaaacatcca acgcttcgac
     1801 gagcggctgc ccgagttgct cgatgcgcta accgaacagg accttctgat catcaccgca
     1861 gaccacggca acgaccctac gttcagaggt acggaccaca cccgcgagag ggtgcctctc
     1921 ctcgtcatcg gcagcggaga gccacacgcc ctcggcattc gcgagggctt ctccgatgtc
     1981 ggagcctccg tagccacttg gctcagagtc gaacagggcg ggctaccggg caggagtttc
     2041 gtgcagggag ccggagattc gtccacccgc tagaatggca cggcggcagc gctgatccaa
     2101 ggcagggacc gtatgggctc agggaacgcg atactcgatg ccatagaggc gaagaagttc
     2161 ggcggagaac tatccgacgg gctcatccgg gacgtggtgg gaggttacgt catgggagag
     2221 gtcccagact accagatggc ctcgctgctc atggctatat tcatccaggg gatgagttat
     2281 ggggagacgc tcgcgctgac gcgggcgatg gccgaatccg gcgagactta ctccttcccc
     2341 gagtgcgtgg acaagcactc cacaggcggc gtcggagaca aggtgagcct gacggcgctc
     2401 cccatcgttg ccgcctgcgg ggcgcccgtt gccaagctct cggggcgggg tctcggctcg
     2461 acgggcggca cgatcgacaa gctggagtct atccccggct tctcgtgcaa cctctctgag
     2521 gagcaattca ggaaccaggt agaggaggtc gggctcgcca taatcgaggc ggcggacctc
     2581 gcgccagcgg acaaggagat c
//
LOCUS       DATMJA010000376         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_110258, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000376 DATMJA010000000
VERSION     DATMJA010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110258"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..807)
                     /locus_tag="VNT12_09000"
     CDS             complement(100..807)
                     /locus_tag="VNT12_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI46296.1"
                     /translation="MTDGETSRELTRVVLADDHPLFREGVASLIGRTDDMALVGEVAT
                     GEEAVCLADDLLPDVVLMDLKMPGMGGTEATRRIVGRSPHIGVIVLTMFEDDESVFAA
                     LKAGARGYVLKDADRGMLLEAIRAVARGEALLGPSVARRVLEQFAQVSPPAPESPPAA
                     PSVGPESFEELTPREVEVLSLIARGLRNREIAGRLVISQKTVGNHVSNIFAKLQVHDR
                     SQAILRALHGGLVQRDE"
     gene            complement(815..>2773)
                     /locus_tag="VNT12_09005"
     CDS             complement(815..>2773)
                     /locus_tag="VNT12_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWI46297.1"
                     /translation="IGLFLTSSGAGGQYAIYAVVSSPGSLPGGGTAAWLEAFLNGPII
                     ITMFALLLLLFPDGRPPSHRWRAVLWLDLIAAALLFVGSFKPGPMETSSLNVANPFGI
                     EQAGALLYALSSVGLYLTLAVTAVGAVSLMVRFRRSRAEERQQIKWFAFAGAIMCAVI
                     ATGPFLWSLPSSSPGVTLIWPLLIFSAVSTIPLATGIAILKYRLYDIDRLINRTLVYG
                     ALTVCIVGIYVLVVGYLGAVFASRGNLVISIFAAGLVATIFQPLRERLQRIVNRLTYG
                     ERDDPYRVLARLGRRLEATIAPEAVLPAIVENIALALRLPQVAVWLADGDTLRLGAAH
                     GSAPTSLTVQDARAVESLRHATDALHPKDLGSSGEYGDVLAGSGVSLILPLTHQGAFV
                     GALCLAPRSPGEEFSPADLLLLRDLATQSGAAAHEVQLTGALRSSLEDLRRSRERLLE
                     AQEEERRRIQRDLHDGLGPLLASMRLRLEACLEVAERTDIPLSGDLERLYELVGEATA
                     DVRLLVYDLRPPVLDQLGLVPAIRQHCERFGRETGIETELATAAELSVPAAAEVTVLR
                     LVQEALVNVQKHARASRAAIRLERQGEWLLVGVADDGKGLSANGGTTAGTGLLSMRER
                     AELLGGRLKLNSRPGGGTDLLARIPVRD"
BASE COUNT          529 a          889 c          891 g          464 t
ORIGIN      
        1 agggcactgg agcattaagg gcaagggatg tccgtcgtcc gtggattgtg cgcagccgac
       61 tcctttgcaa gctagatgca cgagcagggt attctcggct cactcgtcgc gctgtaccag
      121 cccgccgtgc aaggcgcgca agatggcctg gctgcggtcg tggacctgta gcttggcgaa
      181 gatgttggat acgtggttgc ccactgtctt ctggctgatc accagtcgcc cggcgatctc
      241 tcggttacgt agaccccggg ctatgaggct aagtacctcc acctctcgcg gggtcaactc
      301 ttcgaaggat tcgggtccca cggatggtgc ggcaggtggg gactctggcg caggcgggct
      361 cacctgagca aactgctcta gcacccgtcg ggccaccgag ggtccgagca acgcctcgcc
      421 ccgcgccacc gcacggatgg cttccaggag catgccgcga tctgcgtcct ttaggacata
      481 gccgcgggcc ccggccttca gcgccgcgaa caccgactcg tcatcctcga acatcgttag
      541 cacgatgact ccgatgtgcg ggctgcggcc aacgatcctc cgcgtcgcct cggtaccgcc
      601 catgccgggc atcttcaggt ccataaggac gacgtcgggc aacaggtcgt cggcgaggca
      661 aacggcttcc tcgccggtgg caacctcccc gacaagcgcc atgtcgtcgg ttcgaccgat
      721 caggctggcc acgccctcgc ggaagagtgg atggtcgtcg gccagcacca cccgcgttag
      781 ctccctcgaa gtttccccat ccgtcatggc cgcctcaatc cctcaccggg atgcgagcga
      841 gcaggtcggt gccgcccccg ggccgactgt tcagctttaa cctgccaccc agcagttccg
      901 cccgctcgcg catgctcagt agccccgtgc ccgcagtggt gcctccgttg gcagacaggc
      961 ccttaccatc gtctgcgact cccacgagaa gccactcgcc ttgccgctca agccgaatcg
     1021 ccgctcgcga ggcgcgggcg tgcttttgga cgttgaccag cgcctcctgg acgagtcgta
     1081 aaacggtcac ctccgcggcg gcgggcacgg acagctctgc cgctgtggcg agttcggtct
     1141 cgatgccggt ttcgcgtccg aagcgctcgc aatgctgcct gattgctggc accagtccta
     1201 gctgatcgag cactggtgga cgaaggtcat ataccagcaa ccggacgtca gcggtggcct
     1261 ctccgaccag ttcgtagaga cgctccaggt cacccgacag ggggatgtcc gtcctttccg
     1321 ccacctccag gcaggcctca agacgcagcc gcatgctcgc aaggagtggc ccgagcccgt
     1381 cgtgcaggtc ccgctggatg cgtcggcgct cctcctcctg ggcctctaga aggcgctcac
     1441 gcgagcgtcg tagatcctca aggcttgagc gcagggcgcc ggtgagctgc acctcatggg
     1501 cagccgcacc cgattgggtg gccagatccc gcaggagcag caggtcggcc ggggagaact
     1561 cttcgccggg gctacgcgga gccaggcaga gagcaccgac gaacgcaccc tggtgggtca
     1621 acggcaggat caggctcaca ccgcttccgg caaggacatc cccgtattct cccgaggagc
     1681 cgaggtcttt aggatgcaga gcatccgttg cgtggcgtag agactctacg gctcgggcgt
     1741 cctggacggt caggctggtc ggggcgctgc cgtgggccgc cccgagacgc agcgtatcgc
     1801 cgtcggcgag ccagaccgcc acctgcggca accggagtgc cagcgcgatg ttctctacga
     1861 tggccggtag caccgcctcg ggtgcgatcg tcgcctcaag gcgcctcccg aggcgagcga
     1921 gcaccctgta gggatcgtcg cgctcgccgt aggtcaggcg attgactatg cgctggagcc
     1981 tctcacgcag cggctggaat attgtggcga caagtcccgc ggcaaagatg gagatgacga
     2041 ggttgccccg cgacgcgaac actgcgccga ggtagcccac aacgagcacg tagatcccga
     2101 cgatacagac cgtcaaagcg ccgtagacca gagtgcgatt gatgaggcgg tctatgtcgt
     2161 agaggcgata cttgaggatg gcgatccctg tggcgagtgg gatggtgctc actgctgaga
     2221 agataagcaa cggccagatt aaagtgacgc cgggggagga agagggcaga gaccaaagaa
     2281 acggtcccgt agcgataacg gcacacatga tggcgccagc aaaggcaaac cacttgatct
     2341 gttgacgctc ctctgctcgt gatcggcgaa agcgcaccat cagtgagacc gcccctactg
     2401 cggtaaccgc aagcgtcaga tacaacccga cagatgagag cgcatagagc agggcaccgg
     2461 cctgctctat gccgaagggg ttggcaacgt tgaggctcga agtctccatg ggccccggtt
     2521 tgaaggatcc gacaaagagc aacgcggcag cgataaggtc gagccacagt accgctctcc
     2581 agcggtgcga gggcggccgt ccgtcgggga agagcagcaa gaggagagcg aacatcgtga
     2641 tgatgatggg tccgttaaga aatgcctcta gccaggctgc tgtccctccc ccaggaagcg
     2701 aacctgggct gctcaccaca gcgtaaatgg catactgccc gcccgcacct gaggatgtga
     2761 ggaagaggcc aat
//
LOCUS       DATMJA010000377         3681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_406379, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000377 DATMJA010000000
VERSION     DATMJA010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406379"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VNT12_09010"
     CDS             complement(<1..453)
                     /locus_tag="VNT12_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46298.1"
                     /translation="MSKAGDERGMVGERRLLWPSFALPGVLWLLVLFVTPLYAVLAMA
                     MGVTDPIFGDSLPVWNPLRWDPSTFREVLGELFTGQLGVVFLRTLVYVAVATALSLLI
                     GYPVAYYLARHAGRYRVLLLMLIIAPFWINYLMRMLAWVNLLTTDGYVN"
     gene            complement(460..1725)
                     /locus_tag="VNT12_09015"
     CDS             complement(460..1725)
                     /locus_tag="VNT12_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI46299.1"
                     /translation="MAFHPKERSPWRRRMSRREALRLMAGTAAAGGLLAGCGSGAGSS
                     SSASRVVIGTREDPVKQPLFDDNPMIDSGLEPEKGPLRVYNWNEYIYTRVLKDFEKEF
                     GVEVELTTFYNLEEATRKLRTGKVGFDVFVPTAEIIPKFVAGKLFQPLNHDYLPNLKK
                     NVWPMLADPYYDKGSRYTVPYTVYLTGIGWRTDMVDADIAAMDNPWEAFWDPEYKGIA
                     GLYDEYRETIAVGMYKDGITEINGANPEDLKTAQNNLIELTDLVDIRYTIDGAYAKLP
                     EGVFGIHHAWAGDMVYAPYYMPEGADPSVLRYVWPPKAQGGAGGNVSNDSFAVLKDAE
                     HPVLGHMFLNYMLDNKVAIKNFGWNAYQPPINAIDPNTLVSDGTIRENLASTVIVQSD
                     FDMGQIPQQLSPEEDKRWLDAWSRVQQGG"
     gene            complement(1772..2437)
                     /locus_tag="VNT12_09020"
     CDS             complement(1772..2437)
                     /locus_tag="VNT12_09020"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI46300.1"
                     /translation="MGARPKLTQKRRTQMLEAAMEVIAERGLCETRIADVADRAGLSP
                     ALVVYYFGSKETLLTEALAYAEDLFYMDAFRELTGIEGASDRLVRLIELACPPVVRRE
                     SDEYWTLWVELWSRALRNDEAARKREALERRWRNTIAEVVREGQRSGEFGPCDPERFA
                     TYLSALMDGLALQVLFGDPAVNGELANEMCLNAASRELGFDALGLRDEQENSEQEELA
                     AEG"
     gene            2626..>3681
                     /locus_tag="VNT12_09025"
     CDS             2626..>3681
                     /locus_tag="VNT12_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI46301.1"
                     /translation="MRGGYEAIVIGGGHNGLAAGAYLARDGLRTVVLEARHKVGGAAV
                     TDAPWQEAPEFNVTTYSYVMSLMPRRVIDELRLEHHGYKVYPMGPSYYAFPDGRSLTM
                     TGVDAREDHEALARFSRRDAEAYGEWRKWLGELAAIMEPLLLTTPPKIGSRHPLDLLD
                     QLRLAWRMRGLDVPRVGELTRLMTMSAWDLLDDWFESPQIKGAMAVDGIIGTWAGPAT
                     PGTAYVLMHHEIGDAGLGLSSWGYPRGGMGAVSDALKRSAETFGAEVRTNAPVERILQ
                     KDGRVTGVVLEGGEELEADLVVAATHPRITFLEQLGREELPGEFVGSIDRWSSRSGVV
                     KINLALSELPDFTADPGR"
BASE COUNT          661 a         1154 c         1170 g          696 t
ORIGIN      
        1 gttcacgtaa ccgtccgtgg tgagcaggtt cacccaggcc agcatccgca tcaggtagtt
       61 gatccagaac ggggcgatga tgagcatcaa gaggaggacg cggtagcggc cggcgtggcg
      121 ggcgaggtag taggctaccg ggtagccgat cagcaggctc agggcggtgg ccactgcaac
      181 gtagacgagc gtgcgcaaga aaacaacccc gagctggccg gtgaacagct caccgagtac
      241 ttcccggaac gtcgacgggt cccacctgag cgggttccag acgggcagcg aatccccgaa
      301 gatgggatcc gtgactccca tggccatcgc caggaccgca tagagaggcg taacgaagag
      361 gaccagcagc cagagtacgc cgggcagggc gaaggagggc cacaagagac gtcgctcgcc
      421 gaccatccct ctctcgtcgc cggccttgct cacggtcctc tagccgccct gctggacccg
      481 cgaccacgcg tctagccatc gcttgtcctc ctccggcgac agctgctgcg gtatctgacc
      541 catgtcgaag tcgctctgca cgataaccgt cgacgccagg ttctcgcgta tcgtcccgtc
      601 ggacaccagg gtgtttggat ctatagcgtt tatgggtggc tggtaggcgt tccagccgaa
      661 gttcttgatc gccactttgt tgtcgagcat gtaattgagg aacatgtggc ctaggactgg
      721 gtgctcagcg tccttgagca cggcgaagct atcattggac acgttgccgc ccgccccgcc
      781 ctgcgccttc gggggccaca cgtagcgcag caccgaaggg tcggcgccct ctggcatgta
      841 gtagggcgcg tagaccatgt cgcccgccca cgcgtggtgg attccgaaga cgccttccgg
      901 cagcttggca taggcgccgt cgatggtgta acggatgtct accaggtccg tcagctcgat
      961 aaggttgttc tgggcggtct tcaggtcttc cgggttggct ccgttgatct cggtgatgcc
     1021 gtccttgtac atcccgaccg cgatcgtctc cctgtactcg tcgtacagcc ccgctatccc
     1081 cttgtactcg gggtcccaga acgcctccca ggggttgtcc atggccgcga tgtcggcgtc
     1141 gaccatgtcc gttcgccagc cgattccggt caggtagacg gtgtagggaa ccgtgtagcg
     1201 cgaccctttg tcgtagtagg ggtccgcgag catcggccag acgttcttct ttaagttcgg
     1261 caggtagtcg tggttcaggg gctgaaacaa tttccccgcc acgaacttcg ggatgatctc
     1321 ggccgtaggg acgaagacgt cgaagccaac cttgccggtt ctcagcttac gggtggcttc
     1381 ctctaagttg tagaacgtcg tgagctcgac ctcgacgccg aactccttct cgaagtcctt
     1441 gagcacgcgc gtgtagatgt attcattcca gttgtacacg cgcagtggtc ccttttcggg
     1501 ctccagcccg gagtcgatca tcgggttgtc gtcgaacaaa ggctgcttga ccgggtcttc
     1561 cctggtgcct atgaccacgc gcgaagccga cgacgaggag ccggcccccg aaccgcaacc
     1621 ggcgagcagt ccgcccgccg cagctgttcc tgccatcagc ctcagcgcct cgcgacgcga
     1681 catgcgccgc cgccagggag atctttcctt cgggtggaat gccacctcta tcaacctcct
     1741 tttggggcgt ccggctcaga cagccccgtc cctacccctc ggccgcgagc tcctcctgct
     1801 cggagttctc ttgctcgtcc ctcagcccga gcgcgtcgaa gcccaactct ctggaggcag
     1861 cgttcagaca catctcgttg gccagttctc cgttaaccgc ggggtcgccg aacagcacct
     1921 ggagcgcgag gccatccatg agcgcagaga ggtacgtcgc gaagcgctcg gggtcgcacg
     1981 gaccgaactc gcctgagcgc tggccttccc ttaccacctc ggctatggta ttgcgccacc
     2041 ggcgttcgag cgcctcgcgc ttgcgggccg cctcgtcgtt gcgtaaagcc cgcgaccaca
     2101 actcgaccca cagcgtccag tactcgtcgc tctcgcgcct taccaccggc ggacacgcca
     2161 actcgatcaa gcgcaccagc cgatccgaag caccctcgat tcccgtcaac tcccggaaag
     2221 cgtccatgta gaaaaggtcc tccgcataag cgagcgcctc ggtcagaagc gtctctttgg
     2281 acccgaagta gtacaccacc agggctgggc tcaaaccagc cctatccgcc acgtcagcga
     2341 tgcgcgtctc gcaaagaccc cgctccgcga tcacctccat ggccgcctcc agcatctggg
     2401 tgcggcgctt ctgcgtcaat ttaggcctgg cccccattcc cctcccacac tcaatatcca
     2461 aactgtgaac gactatttaa tgtacaacga tagcataagc gcccactcca gtccacatga
     2521 acccgcattc cttggccgaa cagaaccgtc ttgccaccgt aaacgtggga tgctacattg
     2581 aatggtggtt tagaattagt cgcagactct tgagaggaga ggttgatgag gggcggttac
     2641 gaggccatag tcatcggcgg ggggcacaac gggttggcag ccggggccta tctcgcccgc
     2701 gacgggttga ggacggtggt gctggaggcc cgtcacaagg tcggcggggc ggcggtgacc
     2761 gacgcgccgt ggcaggaggc ccctgagttc aacgtcacca cctactcgta cgtgatgagt
     2821 ttgatgcccc ggcgcgtcat cgacgagttg cgtctggagc atcacgggta caaggtatat
     2881 ccgatgggac cttcttacta cgcgttccct gacgggcggt ccctgaccat gaccggggtg
     2941 gacgccagag aggaccatga ggcgctcgcg cggttctcgc gtagggacgc ggaggcgtac
     3001 ggggagtggc ggaagtggct tggagagctg gcggcgatca tggagccttt acttctgacg
     3061 acgccgccga agatagggtc caggcatccg ctcgacctcc tggatcagtt gaggctggcg
     3121 tggcggatgc ggggcctcga cgtgcctcgg gtgggcgagc tcacccggct catgacgatg
     3181 agcgcctggg acctgctcga cgactggttc gagtcgcctc agataaaggg cgcgatggct
     3241 gtcgacggca ttatcgggac gtgggccggt cctgcgactc ctgggacggc gtacgtcttg
     3301 atgcaccacg agatcgggga cgccggcctc ggccttagct cgtggggata ccccagggga
     3361 ggcatgggag ccgtctccga tgcattgaag cgttcggcgg agacgttcgg cgcggaggta
     3421 aggaccaacg cgccggtcga gcgtatcttg cagaaggacg ggcgagtaac gggcgtggtg
     3481 ctcgagggtg gcgaggagtt ggaggctgac ctcgtggtgg cggcgacgca cccgaggatc
     3541 acgttcctgg agcaactcgg acgggaagag ttgcccgggg agttcgtggg tagcatcgac
     3601 cggtggagct cccgcagcgg ggtcgtcaag atcaatctgg cgctctcgga gctacccgac
     3661 ttcaccgccg accctggtcg g
//
LOCUS       DATMJA010000378         5040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_229031, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000378 DATMJA010000000
VERSION     DATMJA010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229031"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /locus_tag="VNT12_09030"
     CDS             <1..1662
                     /locus_tag="VNT12_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWI46302.1"
                     /translation="DVALRFLHEDPWERLARLRERLPNVCLQMLLRGQNAVGYTRYPP
                     DVVRSFLDEARATGVDIFRIFDANNDVEYIRPAIEATLEAGALAEGAFCYTGDLSDPN
                     EDLYTLAYYLRLAEALVEAGSHVLCIKDMAGLVRAPAARKLISALKSEFDLPVHLHTH
                     DTSGGQLATYLAAVEAGVDAIDGAAAPLSGMTSQPSLAAIVAATDHTDRATGLSLEAL
                     GDLEPYWEAVRTLYAPFEAGLRSPTGTIYRHEIPGGQLSNLRQQADAMGLAERFEEVE
                     ALYARCDTLLGRLVKVTPTSKVVGDLALYLISANISMTDFESDPASHDLPDSVIGFLR
                     GELGTPPGGWPEPFRSRALAGRDESPDDDDLSGDDRAALGGEDRRPALNRLMLPGPAG
                     EQAAVRERYGDVSVVPTRAFLYGLEPGEELAVDLEPGVRLYVQLEAITEADGRGIRTL
                     LVTLNGQPRPIDAQDRSLQPQVPAREKADPGEEGHVAAPMTGVVTLAVGEGEEVGGGQ
                     QIGSIEAMKMESAIRAPADGAVQRLAVASGTNVEAGDLLIVLEIG"
     gene            1735..2205
                     /locus_tag="VNT12_09035"
     CDS             1735..2205
                     /locus_tag="VNT12_09035"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HWI46303.1"
                     /translation="MRKGGPLALYALNLFDLSDNDLYRQYSRRSVAAVGKHAGKVVAL
                     GRLDDAVQTQPGVEPRTVMVLVEWPSREAFDAFLEDPEHHDLHPLRERGTQNYLWWTY
                     ERLEDLRPLLKREFAGADLDHSSRNTGDRLRARIVGTRPLRSRSSSHGGAFRKP"
     gene            2242..3849
                     /locus_tag="VNT12_09040"
     CDS             2242..3849
                     /locus_tag="VNT12_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWI46304.1"
                     /translation="MSKLGTFVDRKSEAFEKNAEAFSGLLDELRGRSAEARRGGGEKA
                     QRRHEERGKLPVRERIERLLDPGTAFLELSPLAAFGMYDGRVPAAGIVTGVGRVSGRE
                     VMVVANDATVKGGTYYPMTVKKHLRAQEIAEQNYLPCIYLVDSGGAFLPLQDEVFPDR
                     EHFGRIFYNQAQMSAKGIPQVAAVMGSCTAGGAYVPAMSDEVVIVKGTGTIFLGGPPL
                     VKAATGEDVTAEELGGADVHTRRSGVADHFAENDEHALAIVRSIIENLNREKYVPWTV
                     EKSEEPLYDPGELYGIVPSDYRQGYDVREVIARTVDGSRLHEFKPLYGETLVCGFARI
                     MGHPVGILANNGILFSESALKGAHFIELCCSRGIPLVFLQNITGFMVGREYEAGGIAK
                     DGAKMVMAVANASVPKLTVIVGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGQQA
                     ANVLLTVQEESLRREDKEMSDEEREDFKAPILQKYEDEGNPYHSTARLWDDGIVDPVD
                     TRMVLALGLSAAANAPAEETHYGIFRM"
     gene            3852..4280
                     /locus_tag="VNT12_09045"
     CDS             3852..4280
                     /locus_tag="VNT12_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWI46305.1"
                     /translation="MRAAVVIVENGRVALIERVRDRHTYFKFPGGRVEDGESPQQAAV
                     REAHEELGVSVELGDLICVAYRKGREQRYYLATIAGGTFGTGRGTEMMTSGTTAKGTY
                     RPVWVDLTSLTELDVRPRALSEALASWTGDGEIRWIGEFE"
     gene            4353..>5040
                     /locus_tag="VNT12_09050"
     CDS             4353..>5040
                     /locus_tag="VNT12_09050"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWI46306.1"
                     /translation="MTMSRPDSHNALNAALIEELTRCFEEISDDDRVRVVVLAGEGRS
                     FCAGADIGYMRETALLSYEENLEDARRLAMMYWTIDECPKPVVAKVQGVAMGGGAGLL
                     AVADVVVADSEVRFAFSEIRLGIAPATISPFVLRKIGVSHARSLFLTGERFGAERAHE
                     IGLVHRVVSKDGLDEAVEEKVEELFEGGPVAQATLKGLLRRLETTEPMEAPGLTARVI
                     SELRTGEEGQE"
BASE COUNT          967 a         1534 c         1694 g          845 t
ORIGIN      
        1 gacgtcgcgc tcaggttcct gcacgaggac ccgtgggaga gactggcccg tctcagggag
       61 cgcctcccca acgtctgctt gcagatgctc ctgcgtggcc agaacgccgt cggctacacc
      121 cgctacccgc cggacgtggt gcgctctttc ttggacgagg cgcgcgcgac cggcgtggac
      181 atcttccgca tcttcgacgc caacaacgac gtggagtaca tacgccccgc catcgaggcc
      241 accctggaag ccggggcgct ggcagaggga gccttctgct acaccggcga tctctccgac
      301 ccgaacgaag acctctacac cttagcctac tacctccgcc tggcggaggc gctcgtggag
      361 gcagggtctc acgtgctgtg catcaaggac atggcaggcc tggtgcgcgc cccggcggcg
      421 cgaaagctca tctcggcact gaagagcgag ttcgacctgc cggtacacct gcacacccat
      481 gacacctcgg gcgggcaact cgccacttac ctggcggcgg tggaagctgg ggtagacgcc
      541 atagacggcg ccgcggcccc gctctcgggc atgacgagcc agccgtcgct ggccgcgatc
      601 gtggcggcca ccgaccatac cgatcgcgcg accggcctct cgctcgaagc cctgggggac
      661 ctcgaaccct attgggaggc cgtgcggacc ctgtacgctc ctttcgaggc ggggctccgt
      721 tccccgaccg gcaccatata ccggcacgag atccccggcg gacagctctc caatttgcgt
      781 cagcaggcgg acgccatggg gctcgccgag cgtttcgagg aggtcgaggc tctatatgcc
      841 cgctgcgaca cgttgctcgg ccgccttgta aaggtgacgc ccaccagcaa ggtcgttggg
      901 gacctggccc tgtacctgat ctccgcgaac atctccatga ccgacttcga gtccgatccc
      961 gcgtcacatg acttgccgga cagcgtcata gggttcctgc gcggcgagtt gggcacgccg
     1021 cccgggggat ggcccgaacc gttccgttcc cgggcactcg ccggtcgcga cgagtccccc
     1081 gacgatgatg atctttccgg cgacgatcgg gcagccctgg gaggcgagga ccgcaggccg
     1141 gcgctgaacc ggctgatgtt gccgggacct gcgggggagc aggctgcggt tagggaacgc
     1201 tacggagacg tgtcggtggt cccgacccgg gcgtttcttt acgggctgga gcccggcgag
     1261 gagttggccg tggacctgga gcccggcgtc cggctttacg tgcaactcga agccataacg
     1321 gaggccgatg ggcgtggcat aaggaccttg ctggtcacgc taaacggcca gccccggcca
     1381 atagacgccc aggaccgttc gctgcaaccc caagtcccgg cccgcgagaa ggcggacccc
     1441 ggcgaagaag gacacgtcgc cgcgccgatg acgggagtgg tgacgctcgc ggttggtgaa
     1501 ggcgaggaag tcggtggcgg acagcagatc gggagcatcg aggcgatgaa gatggagtcg
     1561 gccatccggg cccctgcgga cggggccgtc caacggctag ccgtggcctc gggcacgaac
     1621 gtcgaggcgg gagacctgct catcgtgctc gaaatcggat gacgccgaag gggccagcgc
     1681 gcctctcgac gggccgctcg aagccccaat ccacgaacat cttgccagca tccggtgagg
     1741 aaaggaggcc ctttggcact ctacgccttg aacctgttcg acctgtcgga caatgacctg
     1801 taccgacagt actcacgccg ttctgtcgcc gcggtgggca agcatgccgg aaaggtcgtg
     1861 gcgctcgggc ggctggatga tgcggtgcag acgcagcccg gcgtcgaacc gcgaacggtt
     1921 atggtcctgg tcgagtggcc ctcgcgcgag gcgttcgatg ccttcctcga agatcccgag
     1981 caccacgatc tccacccact gcgcgaacga gggacacaaa attacctgtg gtggacttac
     2041 gagcgtctcg aggacctgcg cccgctcctc aagcgagagt tcgcgggcgc tgatctcgat
     2101 cacagcagtc gcaacaccgg agataggcta cgggcacgca ttgtcggaac cagacctctc
     2161 cgctcacgct catcatcgca tggtggcgca ttccggaagc cgtagctaac atgccctcag
     2221 gaaccaggag atcggggagg tatgagcaag ctaggaacct tcgtggatag gaagagcgag
     2281 gccttcgaga agaacgcaga ggccttctcc gggctcttgg acgagttgcg cgggcgctcc
     2341 gccgaggcca ggcgtggtgg gggggagaag gcacagagac ggcatgagga gcgcggcaag
     2401 ctgcccgtgc gcgagagaat cgaacgcctc cttgaccctg gcacagcttt cctcgagctc
     2461 tcgcccttgg cggccttcgg tatgtacgac ggacgcgttc cggcggccgg gatagtcacg
     2521 ggggtcggac gagtctcagg acgcgaagtg atggtcgtcg ccaacgacgc gaccgtaaag
     2581 ggcggtacct attacccgat gaccgtcaaa aagcacctgc gggcgcagga gatagccgag
     2641 cagaattact tgccttgcat ctatctcgtt gactcgggag gcgcgttcct gcctttgcaa
     2701 gacgaggtct tccccgaccg agagcacttc gggcgcatct tctacaacca ggcgcagatg
     2761 agcgcgaagg gtatacccca ggtcgccgcg gtgatgggaa gttgcactgc tggtggggca
     2821 tacgtccccg cgatgagcga cgaggtggtc atagtcaagg ggacgggtac gatcttcctc
     2881 ggcggtcctc ccctcgtcaa ggcggcgacc ggcgaagatg taaccgcgga agagctcggc
     2941 ggcgccgacg tccacacccg ccgctcgggc gtcgccgacc acttcgccga gaacgacgaa
     3001 cacgccctcg ctatcgtccg ctccataata gagaacctta accgggagaa gtatgtgccc
     3061 tggacagtcg agaagtccga ggagcctctt tacgatccag gagaacttta cggcatcgtc
     3121 cctagcgact acaggcaagg ctacgacgtc agggaggtca tcgcgaggac cgtggacggc
     3181 agtcgcctgc acgagttcaa gccgctctac ggcgagacgc tagtctgtgg cttcgccagg
     3241 atcatgggcc accccgtcgg catcctggcc aacaacggca tcctcttctc cgagagcgcg
     3301 ctcaagggcg cgcactttat agagctgtgc tgctcccgcg gcatcccgct cgtcttcctg
     3361 cagaacatca cgggctttat ggtcggcaga gagtacgagg ccggcggcat cgccaaagat
     3421 ggagccaaga tggtcatggc agtcgccaac gccagcgtgc cgaagttaac cgtgatcgtc
     3481 ggcggctcct tcggggctgg caactacggg atgtgcggca gggcctactc gccgcgcttt
     3541 ttgtggatgt ggcccaacgc ccgcatcagc gtgatgggcg gacaacaggc cgcaaacgtc
     3601 cttctcacgg tgcaggagga gagcctgcgg cgtgaggaca aggagatgag cgacgaagag
     3661 cgcgaagatt tcaaggcccc catcctccaa aagtatgagg atgaaggcaa cccataccat
     3721 tccaccgcaa ggctctggga cgacggcatt gtagaccccg tagatacccg tatggtcctc
     3781 gccctcggcc tctccgccgc cgcgaacgcg ccagcggagg agacgcacta cggaatcttc
     3841 agaatgtgat aatgcgcgcc gccgtcgtta tcgtggagaa cgggcgggtg gccctgatcg
     3901 agcgggtgcg cgaccggcac acttacttca agtttcccgg tggcagggtc gaggatggcg
     3961 agagtccgca gcaggccgcc gtgcgcgagg cgcacgaaga gttgggcgtt agcgtagagc
     4021 ttggggatct gatctgcgtg gcttaccgta agggccgcga gcagcgatac taccttgcga
     4081 ccatcgcagg aggaacgttc ggcacgggac ggggtactga gatgatgacg tcggggacga
     4141 ccgcgaaggg aacctacagg ccggtctggg tggatctgac cagcctgact gagctcgacg
     4201 tgcggcctcg ggcgctctcc gaggcgctgg cttcctggac cggtgacgga gagatacgtt
     4261 ggataggaga attcgagtaa tgccgctacg cagagattgg ggtaccgatg cctgattcgt
     4321 tgaaggtaac caggagtggc cctttggcga ccgtgacaat gagcaggccg gattcacaca
     4381 acgcgctgaa cgcggcgctc atcgaggagt tgacgcgctg cttcgaggag atctccgatg
     4441 acgatcgggt tcgggtcgtg gtgcttgctg gcgaaggacg ctcattctgc gccggtgcgg
     4501 acatcgggta catgcgggag actgccttac tctcctacga ggagaacctc gaggacgcaa
     4561 gacggttggc catgatgtac tggaccatag acgagtgtcc gaagcctgtg gtggcgaagg
     4621 tccagggtgt ggcaatgggt ggaggggccg gtctcctcgc cgtagctgac gtggtcgtgg
     4681 ctgattcgga ggtgcggttc gccttctccg agataaggct cggcatcgct ccggccacga
     4741 tctccccgtt cgtcctacgc aagatcgggg tctcgcatgc gagatctttg ttcctcacag
     4801 gggaacgatt cggcgccgaa agggcgcacg agatcggcct agtacacagg gttgtttcga
     4861 aggacggact cgacgaagca gtcgaagaga aagtagaaga gcttttcgaa ggaggaccgg
     4921 tcgcgcaggc gactctcaag ggactgttac gcaggctcga gaccaccgag ccaatggagg
     4981 cgcccggctt gacggcacgt gttatctcgg aactgcgtac cggagaggag ggccaggagg
//
LOCUS       DATMJA010000379         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_821509, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000379 DATMJA010000000
VERSION     DATMJA010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821509"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VNT12_09055"
     CDS             complement(<1..1497)
                     /locus_tag="VNT12_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HWI46307.1"
                     /translation="MRLIVAEKPSVGRDIASALGRHRKDKGYLAGEGWTVTWALGHLA
                     ELAPPDAYGEEYKKWRLESLPILPERFKVRVNHKTREQFNVVKGLMRSPSVKEVVNAC
                     DAGREGELIFAYLYGLSGCRKPVLRLWISSLTHEAIREGFDALRQGTTMKPLEDAARS
                     RGEADWIVGMNATRAYSVRFGGRGNVLSVGRVQTPTLKLLVDREKKIEDFKPEKFWTV
                     HARFARDGSTYDGVWFRKKQNRLTERDAADRIADKVRGGTGTVRKAQKKTATEKPPLL
                     YDLTELQRNANARYGFTAERTLRAAQALYEERKLITYPRTSSRYLSKDLVGGLKKRVE
                     AAGALSDLAPFAEKLLGGQKLPISKRVVDDSKVTDHHAIVPTNKKSSGGLPSDEAKVY
                     DLVARRFVAVFFPAARFENTTVVTEVKEETFLSRGRLVLEAGWRALYPDGVGGRKEKE
                     PPVLPPIEVGQEWAVAKVGVKEGETKPPPRFSESALLGAMETAGKLVED"
     gene            complement(1885..2556)
                     /locus_tag="VNT12_09060"
     CDS             complement(1885..2556)
                     /locus_tag="VNT12_09060"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWI46308.1"
                     /translation="MSEHPDIRDLLGPYVMGTLEPHEEREVEDHLGGCASCREEAQEL
                     RLAHERLADLAYSTGTPPEDLKARVVAGIPRREASRRIPTWVAAVAAALCILAVLPVI
                     LTPDLFGGGALASADLSPTDRAPDAGGEVSIRGTGENMEMRLEVWGLPPCKGEQYYEL
                     WLVEGDERVSAGSFTVGQSGRVDVTLSAPNFAASYPSVGVTAEYDKDPRASGAKMLSG
                     ELHEL"
     gene            complement(2549..3235)
                     /locus_tag="VNT12_09065"
     CDS             complement(2549..3235)
                     /locus_tag="VNT12_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI46309.1"
                     /translation="MERLVSGSAECLAPDHGRGWDIICSVWRETGLSVLAGGRGRFWM
                     DRIEDEVLVRRIASGDERALSGLYDRYAGLVYGTGMRYLGDRETAEELVQDVFTSVWK
                     NAAGFDPTRASFATWVYRITRNRATDLIRRRKARVRTVDGESLFEPGETDPSGELSRN
                     FDVAAALSRLTPAHREVLILAYFYGFSQREISDRTATPLGTIKSRTTAALRALRENMR
                     FAEDRWSADE"
BASE COUNT          572 a         1152 c         1021 g          660 t
ORIGIN      
        1 gtcctcgacg agcttccccg cagtctccat cgccccgagc agcgcggact ccgagaagcg
       61 cgggggcggc ttggtctcgc cctccttgac gccgaccttc gcaacggccc actcctgccc
      121 cacctcgatc ggcggcaaca cgggtggttc tttctccttc cgccctccca ccccgtcggg
      181 gtagagcgcg cgccaccccg cctcgagcac caggcgccca cgggaaagga acgtctcctc
      241 cttgacctcg gtcacgacgg tggtgttctc gaagcgggca gcggggaaga acacggccac
      301 aaagcgcctg gccaccaggt cgtagacttt cgcctcatcc gacggcagcc cgcctgaaga
      361 cttcttgttc gtcgggacga tggcgtgatg gtcggtaacc ttggagtcgt ccacgacgcg
      421 cttgctgatc gggagcttct gtcctccgag cagcttctcc gcaaaaggcg caaggtcgga
      481 gagcgcccct gcggcctcga cccgcttctt caggccacct accaggtcct tggagaggta
      541 gcggctcgag gtgcgcgggt aggtgatcag ctttctctct tcgtatagcg cctgggcggc
      601 ccgcaacgtg cgctcggccg tgaagccgta gcgggcgttg gcgttgcgct ggagctcggt
      661 cagatcatag aggagaggcg gcttctcggt tgccgtcttc ttctgcgcct tgcggacggt
      721 ccccgtcccg ccgcgcacct tgtccgctat ccggtctgcg gcatctctct cggtcaggcg
      781 gttctgcttt ttcctgaacc aaacgccgtc gtaagtactc ccgtcccgcg cgaagcgggc
      841 gtggacggtc cagaactttt cgggcttgaa gtcctctatc ttcttctcac gatccaccag
      901 aagcttcagg gtcggggtct ggacgcggcc cacagagagt acgtttccgc ggcctccgaa
      961 tcggaccgag taggcgcggg tggcgttcat gccgacgatc caatcggcct ccccgcggct
     1021 gcgggccgcg tcttcgaggg gcttcatcgt agtgccctga cgtaatgcgt cgaaaccctc
     1081 ccttatagcc tcgtgggtga gagaggagat ccagagccgt agaacaggct tcctacaacc
     1141 cgagagaccg tacaggtagg cgaagatgag ctctccctct ctccctgcgt cgcaggcgtt
     1201 tacaacctcc ttcacagaag ggctcctcat cagacccttt acgacgttga actgctcgcg
     1261 ggtcttgtgg ttcaccctga ccttgaaacg ttctggcagg atcggcaggc tctccaaacg
     1321 ccacttcttg tactcctcgc cgtaagcatc aggcggtgcc agctcggcca ggtgcccaag
     1381 agcccaggta acagtccacc cttcgccagc cagatatcct ttatctttcc gatgcctccc
     1441 cagggcactc gctatgtccc ggcctacgga cggcttttcg gcgacgatga gcctcataca
     1501 gaggagtata cgtacgggcg ggacgatagc cagatgagat tgtctgagac gcggttggtc
     1561 aacgagcgga tggctctggc ggcgccggag atggatgcac cttagcacct cgcggccgtc
     1621 caggcggggc agcgaggagg accaagtcca ccagctcgtc cacggccagc ctcgtgcccc
     1681 cctcgccgtc gtccgcgcag acgaccctgt agccgtgtgc gttcaaaccg cgctccagga
     1741 agcgcgcaat cgcggcctcg tcctcgacga tgtcccgtat ggtcgaggac ctgcacttcc
     1801 tggcccgttc ggagtcggcc gcctttccgc atggagcccg tggacgcggg ggtcctgctc
     1861 gcgggcctcg agcgtcggtt gatttcagag ttcgtgcaac tcgcctgata gcatcttcgc
     1921 gccactggcg cgggggtctt tgtcgtactc ggcagttacc cccacactgg ggtaagaagc
     1981 ggcgaagttc ggtgcgctca gcgttacgtc gacccggccg ctctgtccga cagtaaagct
     2041 ccccgcgctc acccgttcat cgccctcgac gagccacagc tcgtagtact gctcgccctt
     2101 gcagggaggc agcccccaca cttcgaggcg catctccatg ttctcgccgg tgccccggat
     2161 gctcacctcg cctccagcat cgggggcgcg gtcggtgggg ctcaagtccg ccgaggccag
     2221 ggccccgcca ccgaagagat caggggttag gatcaccgga agcaccgcga gtatacagag
     2281 ggccgcggcg accgcggcca cccaggttgg gatgcgtcgg ctggcttcac gccgcggtat
     2341 ccctgccacg accctagcct tgagatcctc cggcggtgtc ccggtgctgt acgcaaggtc
     2401 agcgaggcgt tcgtgggcca gtcgtagctc ttgagcctcc tccctgcaac tcgcgcatcc
     2461 tcccaggtga tcctctacct cccgctcctc gtgcggttcg agggtgccca tcacgtaggg
     2521 gccgaggaga tctcgtatgt caggatgctc actcatcggc tgaccaccga tcctcggcga
     2581 atctcatatt ctcgcgcagg gcgcgcagtg ccgccgtggt gcggctcttt atagtaccca
     2641 ggggcgtagc ggtgcggtcg gagatctcac gctgcgagaa gccgtagaag tacgcgagga
     2701 tgagcacctc cctatgggcc ggagtgagcc gcgaaagcgc ggcggccacg tcgaagttcc
     2761 gtgagagctc gccagaaggg tctgtctctc ctggctcgaa gagcgattcc ccgtcgacgg
     2821 tcctgacacg cgccttgcgg cgacggatca ggtccgtcgc cctgttgcgg gtgatgcggt
     2881 acacccaagt ggcgaagctc gcccgcgtgg ggtcgaagcc agccgcgttc ttccacactg
     2941 aggtgaagac gtcctgaacc agttcctcag cagtctccct gtctcccagg taacgcatcc
     3001 ccgtcccgta gaccagcccg gcgtagcgat cgtagagtcc agagagggcg cgttcgtccc
     3061 cagatgcaat ccttcgcacc agaacctcgt cttctatacg gtccatccag aaccttcccc
     3121 gaccgccggc gagaacggag agtccggtct cgcgccacac ggagcagatg atatcccatc
     3181 cgcgcccgtg atccggcgcg agacattcgg cactccctga taccaacctt tccatacaga
     3241 catttatacg accggccaag cataccggat tagcttcttc gccggccacc gtattgttgc
     3301 caaaccacaa actcagggga actcaggttt cgtgctctac tctccgagag cagcctcgac
     3361 cggttagaat tggcgagatc cttgccgtag ttctcggcta gcagc
//
LOCUS       DATMJA010000380        10292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_465864, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000380 DATMJA010000000
VERSION     DATMJA010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465864"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VNT12_09070"
     CDS             <1..646
                     /locus_tag="VNT12_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWI46310.1"
                     /translation="ILVRMIWVFPATYVPRWTIRRIRERDPSPSWRSVSVIAWTGMRG
                     VISLAAALALPLQTTAGGQFLDRNLIIFLTFCVILATLVLQGLSLPVLIRTLGLEDDH
                     IGDKEETHGRIQVAEAALERLDELVDEDWVREDTAERVRGLYSYRRNRFASRFDGDPD
                     GVEVRSAAYQRLMIELLGAQRLRLVTMRDEGSIGDEVMHRIERDLDLEESRLEL"
     gene            complement(722..1111)
                     /locus_tag="VNT12_09075"
     CDS             complement(722..1111)
                     /locus_tag="VNT12_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HWI46311.1"
                     /translation="MIAARLQEGDEVRVVSPAVSLGFIPEDQKSLARERLEGLGLRLS
                     FSTNAEVMDRFDSSPVGARVSDLHEAFADSGVRGILTTLGGYNSNGLLGYLDYDLIRA
                     NPKIFCGFSDITALATAIIARFFIDVH"
     gene            complement(1124..2146)
                     /locus_tag="VNT12_09080"
     CDS             complement(1124..2146)
                     /locus_tag="VNT12_09080"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWI46312.1"
                     /translation="MISGTHTFLQTTRVHFGADALEKLEQEAQPYDRAFVITGRSLNE
                     KTGLISRVEALLDGRHAGTFAGIGQHTPGGAVEKAAVEAEMTAADLLVGVGGGSVIDG
                     TKAVARELGYPQQVAIPTTLSGAEWANRAGVTDERSGRKRGFADPKTVPRVVVLDPEA
                     TVFTPEKLWLSTGIRALDHAVEGYLFGGDHPITDVTGLEGARRLMKYLPLSKREPEDL
                     ETRLELQLAAWLSYFGPANTSMGLSHELGRRIGASYEVPHGITSCIILAPSLRVVKER
                     VQDERWHMLSQALGGEPPERVSSLVEELRLPGRLREVGVPEQDLEDIAGEYGDGEALA
                     ILKAAF"
     gene            2536..2682
                     /locus_tag="VNT12_09085"
     CDS             2536..2682
                     /locus_tag="VNT12_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46313.1"
                     /translation="MDGGLDLEYLARALLAPLDVDLFYYQHRVQGLPAERISGGVRDL
                     VPRA"
     gene            2694..3695
                     /locus_tag="VNT12_09090"
     CDS             2694..3695
                     /locus_tag="VNT12_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HWI46314.1"
                     /translation="MTDRRIEMELGGLHHMTAVTTNAAENVAFYTDVLGLRLVKKTVN
                     QDDVSAYHLFYGDEVGNPGTEITFFDWPHLRVAPNHPGAGEVSTTELRVPDRAALDWW
                     AARFQDLGVDNDGIVEGENRTYLAFTDPEGQRLRLSADEGEGVQGGVPWAESPVPREM
                     GIRGLGAVDLTVRESDPTAWVLTEVLGFRKVGEIANSGGRTITFEVGGGGPGATVRVI
                     ERPNAPWTHLGRGGVHHIAFRTPDDEEHAAWRERIRRSGLGVTPQIDRFYFRSIYFRE
                     PGGVLFEIATDGPGFATDEDAAHLGEKLSLPPFLEERREEIEAGLDPITIEDETARR"
     gene            3720..4376
                     /locus_tag="VNT12_09095"
     CDS             3720..4376
                     /locus_tag="VNT12_09095"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI46315.1"
                     /translation="MPASIASRDSDKYPYPQWLRWSRRLQAIAQDGLTYARDSYDLGR
                     YEQLRDLAAEILAAHSTGTLEEARDLLALETGPATPKVDVRAAVFREGRVLLVKEPGE
                     GGWALPGGWADVGEAPSEAAARESLEESGYRVRPVRLLAAYDRDRHGHPPIPYHVYKL
                     VFLCEILDKTPSPDVDTEGVGFFGEEQLPELSISRVTRAQISRFFEQHRDPDRPADFD
                     "
     gene            4867..6117
                     /locus_tag="VNT12_09100"
     CDS             4867..6117
                     /locus_tag="VNT12_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWI46316.1"
                     /translation="MRERLRNRPRLRPRVYGYLTEEKETLRQGFAALFLSSAGELIAG
                     IVLAGISGTLNDLVGLAVLIPAAIGMRGAIFGAMGSRLSTSIQTGLFRFDLRRGVLAE
                     NVQAAAVLSLVSGVFLAFLARVLCGILGVRTELSVIDYVVISTAGGIIAGVLLLGITV
                     FVARLSVARGWDMDNIAAPMLTAAGDILTLPSLVLATYLIGIPVFSSSLGVALVLAAV
                     AAAYFGLRVGVENLRRILAESFPILALTGTVTILVGVALQHREEQFTTLLALSILLPA
                     FLQEGGALGGILSSRLSSKVHLGLIAPSGIPQFAALRDFTLTYIFAAGVYVFIGGASH
                     LIAVALFEEGVSPGFLAMIGISALAGLIATTAAVFAAYYGSTLSYRLGLDPDTYGIPI
                     ITAAVDLLGFMSLIIALIVFGLAG"
     gene            6121..7320
                     /locus_tag="VNT12_09105"
     CDS             6121..7320
                     /locus_tag="VNT12_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWI46317.1"
                     /translation="MRETRPRNVKEMLVEAKNTSELMVDLSYAAIFYNSEDLSEEVSH
                     LEERLNDLVFDMRSLTILAARSPADAEQMAGILQVVQDIEKIGNAAVDIAKIVVKRLG
                     IPPELLHDIPEAEEIPSRVRVHPDSALDGRSLGDVDLPVETGMSPIALRSEDVWTFDP
                     EPEDVLRAGDVLFLQGPPEGVAEVRELAGARPLGDAPEPEPTPGELSEIDRAVDILIE
                     MKNLSEVAVGLAYSALLYDDAGLAREVVAIEDEMDEMRYRLERWVLLAAPRVEDSSRL
                     RGLLHLATASETIADCAMEMVWIVEKGEEVHPVLSAAIGESDEIVLKLTVSPGSPADG
                     KTLDSLKIETETGMYALAVNRGGRWTYRPRDTYRLQGGDSLLVTGAHEGLEPLAELFG
                     QELEESP"
     gene            7418..9283
                     /locus_tag="VNT12_09110"
     CDS             7418..9283
                     /locus_tag="VNT12_09110"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HWI46318.1"
                     /translation="MKRKALLVGLLLLLLCTMGFARGSQMDETEVLEQASLIPTLEEY
                     YSKPTVETTADYDGGKDLWRVVLTEQTSGKEVARFKVADDSGKVRGVKVSPRADEIKY
                     PKLSEDKAIKLAAASPEVREEFTRHGPHSGEAKYEEGAWTVRFYVDERGAIGGRPTEK
                     GKEIATVGVDDKTWVLDYVYTGDQVGWNLARGVRGAYGKQANYWWVWLPLALAFAAAF
                     WRTDRLFAMRNVDIVALLGFLVSHGFFREGVVLEAVVLWYPPLVYLLIRTLLMGFGIG
                     EKVEKTSNLPMWVLMVLAGLAGGLVLGLNVDSRVIDVGYAGVVGADRILEGTAPYGTM
                     PSDVGTGDTYGPLNYLLYVPFVLMFGFSGEWDFLPAAHALTLFSFVAGAMALFITGYR
                     LSGKEGAAALVFAWAAFPYTVYATNNNTNDIIVAAVSAIGLAAAASPIARGASIAAGF
                     AIKLYPLVLGPLWIMYEGRKRKPIIDFVLGGSGVVLVTFWVILLDGHPIEAARRFYDN
                     TLAFQDNRVSPWSLFSQVPQLAFLQRPLVAFAIFLSILVAFVPREKTLRRLAALSAAL
                     VIAFQLTVNYWFYAYITWFEPFVFVSLLLATNQKSALDSSQPSAVSDQPQEEVPT"
     gene            9285..9599
                     /locus_tag="VNT12_09115"
     CDS             9285..9599
                     /locus_tag="VNT12_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46319.1"
                     /translation="MGDASENAERPILNVEGNLVALGPLGRDLLPLYQRWINDLGTMR
                     TLGLAPLPMTSEKEQDWYDRQSKAEDDVPFTIYERETAPEAGWGRYRLLVGPRKMGQR
                     IA"
     gene            9988..>10292
                     /locus_tag="VNT12_09120"
     CDS             9988..>10292
                     /locus_tag="VNT12_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46320.1"
                     /translation="MIGRIWHGWTIPQNADKYERLLKEEIFSGIADKKVSGYKRIQLF
                     RRPLDNDEVEFVTIMWFDSWDAVKQFAGDDYERAYVPAAAQEVLARFDERSQHYEIR"
BASE COUNT         1867 a         3232 c         3214 g         1979 t
ORIGIN      
        1 catcctcgtg cgcatgatct gggtcttccc agctacgtat gtcccaaggt ggaccatacg
       61 ccgcatcagg gagcgcgatc cctccccttc ctggcgcagt gtctcggtta tagcgtggac
      121 cgggatgcgg ggtgttatct cgctggcagc ggccctcgca ctgccgttgc agacgaccgc
      181 ggggggacag ttcctcgacc gcaacctcat catcttcctg acattctgcg tgatcctggc
      241 tacgctcgtg ctccagggcc tctccctacc ggtcctgatc cgaaccctcg gcctcgagga
      301 cgaccacatc ggggacaagg aggagactca cggccgcatt caggtcgcgg aggccgccct
      361 ggagcgcctg gacgagcttg tcgacgagga ctgggtgcgc gaggacacgg ctgagcgcgt
      421 tcgcggcctc tacagctacc gccgcaaccg cttcgcctcc cgcttcgacg gcgatcctga
      481 cggcgtcgaa gtacgctccg ccgcctacca gcgcctcatg atcgagctgc tcggggcgca
      541 gcgcctgagg ctcgtcacga tgcgcgacga gggtagcatc ggggacgagg tcatgcaccg
      601 catcgagcgc gacctcgacc tggaagagtc ccgtctagag ttgtgatgct agctggtcag
      661 cacggcggct ccgccgtgtt gaccagcgaa gttttaggaa gttctctacc tgaaactgct
      721 ttcagtggac gtcgatgaag aacctggcga tgattgctgt tgcaagagcc gtaatgtccg
      781 agaaaccgca gaagatcttg gggttcgcac ggataagatc gtagtccaga tacccgagta
      841 gcccattgct gttgtagccg ccgagggtgg tgagtatacc tctgaccccg gaatccgcga
      901 acgcctcgtg cagatcggag actcgcgccc ctaccggtga ggaatcgaag cgatccatca
      961 cctcggcgtt tgtggagaac gagagacgga ggcccagacc ttcgagccgt tccctggcca
     1021 gcgacttctg atcttctggt atgaacccga ggctgaccgc cggtgagacg acccttacct
     1081 catcaccctc ctggagtctc gcggcgatca tggcccgcgc atcctagaac gcggccttca
     1141 ggatcgccag tgcttccccg tcgccgtact ctccggcgat gtcttccagg tcctgctcag
     1201 gcaccccaac ctcacgaaga cggccgggta atctgagttc ctccaccagc gacgagacgc
     1261 gctcgggcgg ttctccgcct agagcttggg agagcatgtg ccagcgctca tcctgtacgc
     1321 gctctttcac gacccgtagg cttggtgcga ggataataca ggaggttatg ccgtgtggga
     1381 cttcgtagct ggccccgatc ctgcgcccca attcgtgcga gagacccatc gacgtgttcg
     1441 ccggcccgaa gtatgagagc caggccgcga gctgtagctc caggcgcgtc tccaggtctt
     1501 cgggctcgcg cttggaaagc ggcagatact tcatcaggcg ccgcgctcct tcgagaccgg
     1561 ttacgtcagt gatgggatgg tcgcctccga agagatagcc ttcgaccgcg tgatccaagg
     1621 ccctaatgcc ggtcgagagc cagagcttct ctggcgtgaa gacggtggcc tcagggtcca
     1681 gcacaacgac cctcggaacg gtcttcgggt cggcgaaacc tctcttccta ccggatctct
     1741 cgtccgtaac ccctgctctg ttggcccact ccgcaccgga gagggtagtc gggatcgcca
     1801 cctgctgagg atagcctagc tcccgcgcaa ccgccttggt cccgtctatg acgctacccc
     1861 caccgacgcc gaccaggagg tccgccgccg tcatctccgc ctcgacggct gccttttcca
     1921 ctgccccacc gggggtatgc tgcccgatac cggcgaaggt ccccgcgtgc ctgccgtcaa
     1981 gcaacgcctc gacgcggctg atgaggccgg tcttttcgtt caggctgcgc ccggtgatga
     2041 cgaaggcccg gtcgtacggc tgggcctcct gttccagctt ctccagagcg tccgcgccga
     2101 aatgaacgcg cgtggtctga aggaaggtgt gggtaccgct tatcacgctc ctatcgtacc
     2161 gctacgcgct actccaggcg accgtgcgca cgtcagcctt tcggaggagc ccagcgttct
     2221 cccagccgtt ggcgcggaga tattgcagcc cggcgagctc tcgcctccgg cttttcggga
     2281 tctcgggtga gcgcacgtac cccgattccg cgttcgagta ggctccgcgc caccgctccc
     2341 ccttgctggc ccgttgctcc gctaacaagg atcaaacgcc cctcgttgcg cccgtcttcc
     2401 atacttcctc cttcactctg atctctctac gaccgactac actacaaaaa cggccgtacg
     2461 tccactccaa agaatgaggg tagcgcggta gactgccggt atcagggtca tgatggagag
     2521 tggtagtctt gaggcgtgga cggtggtctt gacctcgagt acctagctag ggcgctgctc
     2581 gcaccgctcg acgtggacct gttttactac cagcacaggg tacaggggct tcctgccgaa
     2641 cgaataagcg gcggtgtgcg tgacctcgta ccccgcgctt gatgagacgt gagatgaccg
     2701 ataggaggat agagatggaa ctgggcggcc tgcaccacat gacggcggtc acgacgaacg
     2761 ccgcagagaa cgtggcgttc tacacggacg tgctcgggct aaggctcgtc aagaagacgg
     2821 tgaatcagga cgacgtctcc gcctaccacc tcttctacgg agacgaggtt ggcaatcctg
     2881 ggaccgagat caccttcttc gactggcccc atctgcgcgt ggcccccaac catcccggcg
     2941 cgggcgaggt atcgactacc gagttgcgcg tccccgaccg cgcagcgctc gactggtggg
     3001 cagcgcggtt ccaggatctc ggcgtggata acgatgggat cgtagaagga gaaaaccgca
     3061 cttacctcgc cttcaccgac ccagaagggc agcgactcag gctctcagct gacgaggggg
     3121 aaggagtaca gggcggtgtt ccgtgggcag agagccccgt tccgcgagag atggggatca
     3181 ggggtcttgg ggcagtcgac ctgacggttc gggagtcgga tcccaccgcc tgggtgctca
     3241 ccgaggtgct cggctttcga aaggtgggcg agatcgcaaa tagcgggggc cggacgataa
     3301 ccttcgaggt tgggggtggc ggtccggggg cgacggtgcg ggtgatcgaa cgccccaacg
     3361 caccgtggac ccacctggga cgtggcggcg tgcatcacat cgctttcagg acgcccgacg
     3421 acgaagagca cgcggcgtgg agggagagga tccggcgctc aggactcggc gtaacgccgc
     3481 agatagaccg cttctacttc cgttcgatct acttccgcga acctggcggg gtgctattcg
     3541 agatcgccac cgacggccct ggcttcgcca cggacgagga cgccgcccac ctcggcgaga
     3601 agctttccct ccccccgttc ctggaggagc gcagggaaga gatagaggcg gggttagatc
     3661 ccataaccat agaagacgag acagcacgac gttgaatccg ttgtggattc cgctagagaa
     3721 tgccggcaag catagcttct agagacagcg ataaataccc ctacccacag tggctgcggt
     3781 ggagcaggcg cctgcaagcc atagcccagg acggcttgac ctatgccaga gacagctacg
     3841 acctcgggcg ctacgagcag ctcagggatc tggcagccga gatactcgca gcccattcca
     3901 cagggacact ggaggaggcc agagatctgc tcgcgctcga aaccggtcct gcgaccccga
     3961 aggtggacgt tcgggccgcc gtgttccggg aagggcgagt cctgctggtg aaagaaccgg
     4021 gcgaaggggg ttgggcgttg ccaggaggct gggcggacgt gggtgaagct ccgtcagaag
     4081 ccgcggctcg cgagtccctg gaggaatcgg gttacagggt gcggcctgtc aggctgctcg
     4141 ctgcctacga ccgcgacagg cacggccatc cgcccatccc ctaccacgtc tacaagctgg
     4201 tgtttttatg cgagatcctg gacaagactc cgtcacctga cgtggatacc gagggggtgg
     4261 gcttcttcgg cgaggagcag cttccggagc tctcgatctc ccgcgttacc cgggcccaga
     4321 tctcacgctt cttcgaacaa caccgcgacc cggaccggcc cgccgacttc gactagggcg
     4381 gtttgccagc tgagggattc cacttttaag cgtcgggagc cccgcagtgc cgctgatttg
     4441 gtctgcatct atactctcct tttcgaaggg atgctgcctc acggatcaaa cctcggcttg
     4501 ctgcggggac gaaatcacgc ccagctgctg catcaggctc atcatttcca actgtccccg
     4561 gaaattcacg attcttccgt cgacgatccg gtagaagacg ctgcccggtg cttcgccgcg
     4621 cttgccggtt ggagggatgc cctgcagctc acccaggtgt gtgccccgac ctgtccaggg
     4681 cgcctttacc tcgacgcgcg gtctcagcct tgcgtcgttt gttttagatc ggtgagccag
     4741 gctgctgctg gctgacatcc tccctcacca ccgctttact tcgctggctg cagagactat
     4801 acattccccg tgtccgagag agagaaaagg cgagtccggt ggcgagaggg cggccgggag
     4861 ggcggggtgc gcgagaggct gcgcaacagg ccccgtctgc gtccgagggt ctacggctac
     4921 ctcacagagg agaaggagac tctcagacag gggttcgcgg cgctgtttct ctcctcagcg
     4981 ggggaactca tcgcaggcat cgtgctcgca ggcatctcag gcacgctgaa cgacctcgta
     5041 gggctcgctg tcctgatccc cgcagccata gggatgcgcg gcgccatttt cggggctatg
     5101 ggaagcaggc tctcgacctc catccagacc ggcctattca ggttcgacct caggcgcggc
     5161 gtgctcgccg agaacgtaca ggccgcagcc gttctctcgc tcgtctccgg ggtgttcctc
     5221 gcttttctgg cgcgagtcct ttgcggcatt ctcggcgtca gaacggagct ttcggtcatc
     5281 gattatgtcg tcatatccac ggccggcggt atcatcgccg gcgtgctgct gctcgggatc
     5341 acggtcttcg tcgcccgcct ctcggtcgcc cgcggctggg atatggataa catcgcagcc
     5401 cctatgctca ccgctgccgg ggatatcctc accctcccct ctctcgtcct ggctacctac
     5461 cttatcggca tccccgtctt ctcttcttcc ctcggcgtcg cgctggttct ggccgcggtg
     5521 gccgcggcat acttcggcct cagggtaggc gtcgagaacc tgaggcgtat cctggctgaa
     5581 tcgttcccta tcctggccct gacagggaca gtgaccattc tggtcggcgt ggcgttgcag
     5641 catcgggagg aacagtttac tacgcttctg gccttgagca tccttctgcc cgccttcttg
     5701 caggagggcg gcgccctcgg cggcatcctc tccagccgcc tctcctccaa ggtccatctc
     5761 ggtctcatag cgccaagtgg gataccacag ttcgcggcct tgagagactt cacgctcacc
     5821 tacattttcg ccgccggtgt ctacgtgttc atcggggggg cttcgcacct catagccgtg
     5881 gcacttttcg aagagggtgt cagtccaggc ttcctggcga tgatcgggat cagcgctctt
     5941 gctggtctga tcgccaccac ggcggcggtc ttcgcggcct actacggctc gaccctcagc
     6001 tacaggctcg ggttggaccc agacacctac ggcatcccga taatcacggc ggcggtggac
     6061 ctgctcggct ttatgagcct tataatagcc cttatcgttt tcggattggc ggggtaagag
     6121 atgcgcgaga cgcgaccacg gaacgtgaag gagatgctcg tcgaggcgaa gaacacctcg
     6181 gagcttatgg tcgacctctc ctacgcggcg atcttctaca actccgaaga cctctcggaa
     6241 gaggtatccc acctcgagga gcgcctcaac gatctggtct tcgacatgcg ctccctgacc
     6301 atcctggccg cgaggagccc cgcggacgcg gaacagatgg cgggaatcct ccaggtcgtc
     6361 caggacatag agaagatcgg caacgccgcc gtcgatatag ccaagatcgt ggtcaagcgt
     6421 ctaggtatcc cgcccgagct cctgcatgat atcccggagg ccgaagagat accctcccgt
     6481 gtccgcgtgc accccgatag cgcccttgac ggccgctctt taggcgacgt tgacctgccc
     6541 gtggagacgg gaatgagccc catcgccctc cgctccgaag atgtctggac gttcgatccc
     6601 gagcccgaag acgtccttag ggccggcgac gtactgttcc tgcagggccc gccggagggc
     6661 gtcgccgagg tcagggagct cgctggcgcc cgtccgctcg gagacgcgcc ggagcctgag
     6721 ccaacccctg gcgagctctc ggagatcgac cgcgcggtgg acatcctgat agagatgaag
     6781 aacctctcag aggtagcagt cgggctcgcg tactcggccc tgctctacga cgacgcgggg
     6841 ctcgcccgcg aggtggtcgc catcgaggac gagatggacg agatgcgcta ccggctggag
     6901 cgctgggtcc ttctcgccgc cccccgtgtg gaggactcct cgcgcctcag gggcctcctt
     6961 cacctcgcca ccgcctcgga gaccatcgca gactgcgcca tggagatggt ctggatcgtc
     7021 gagaaaggcg aggaggtcca tcctgtgctc tccgcggcga tcggcgagtc ggacgaaatc
     7081 gtcctgaagc tcaccgtctc cccaggctcc ccggccgacg gcaagacgct tgactctctc
     7141 aagatcgaga cggagacggg gatgtacgcc ctcgccgtga accggggcgg acgctggacc
     7201 tacaggcccc gtgacaccta caggctgcag ggcggcgact cgctgctcgt tacgggcgcc
     7261 catgaaggcc tcgaacccct cgccgagctc ttcggccagg agctggagga gtccccgtaa
     7321 tccgggaagg ccgccaagag tatcacacca tccggggtca gccgctgccg gcaacattca
     7381 tatgcccgct cgccctgcca acaccgataa ggtcctcgtg aagcgtaagg cattgctggt
     7441 ggggctcctg ctgttgctgc tgtgtactat ggggttcgcg cgtggttccc agatggacga
     7501 gacggaggtg ctggagcagg ccagcttgat cccgacgctg gaggagtact actccaaacc
     7561 gactgtcgag acgacggcgg actacgacgg gggcaaggac ctctggcggg ttgtccttac
     7621 ggagcagact tctgggaagg aggtcgcccg tttcaaggtc gccgacgatt ccggaaaggt
     7681 gagaggggtc aaggtctcgc cgagagcgga tgagatcaaa taccccaagc tctcggaaga
     7741 taaggcgatc aagctcgccg cagcgagccc cgaggtcagg gaggagttca caaggcacgg
     7801 acctcacagc ggggaagcca agtacgagga gggcgcgtgg acggtccgct tctacgtcga
     7861 cgagaggggg gccatcggag gcaggccgac ggagaagggc aaagagatag ccaccgttgg
     7921 ggtggacgat aagacctggg tcctcgacta cgtttacacg ggggatcaag tcgggtggaa
     7981 cctggccagg ggcgtgcgcg gcgcctatgg caagcaggcc aactattggt gggtctggtt
     8041 accactggcg ctagccttcg ccgctgcttt ttggcgcacg gacaggctgt tcgccatgcg
     8101 aaatgtggat atcgtggcgc tgctcgggtt cctcgtctcg cacggtttct tcagggaggg
     8161 ggtagtcctc gaagccgttg tcctgtggta cccgccgctc gtctacctcc tcatcaggac
     8221 ccttctcatg ggtttcggga taggcgagaa ggtcgagaag acgagcaacc taccgatgtg
     8281 ggtcctgatg gtgctcgccg gtcttgccgg cggcctcgtc ctcggcctca acgtcgactc
     8341 ccgcgtcata gacgtaggct acgccggggt cgtcggcgcc gaccgtatcc tggaaggcac
     8401 agcaccctac ggcaccatgc cctcagacgt gggaacaggg gacacttacg gccctctaaa
     8461 ttacctgctc tatgtgccct tcgtcctcat gttcggcttc tccggagagt gggacttcct
     8521 ccccgcagcc cacgccctca cactgttctc cttcgtcgcg ggggcgatgg cgctattcat
     8581 cacgggctac aggctctcgg gcaaggaggg ggccgccgcc ctcgtcttcg cctgggcagc
     8641 cttcccctac accgtttacg cgaccaacaa caacaccaac gacatcatcg tcgccgccgt
     8701 ctcggcgata gggctcgctg cggcggcctc gcctatagcg cggggggcga gcatcgcggc
     8761 cggcttcgcc atcaagctct atccgctcgt cctgggaccc ttgtggatca tgtacgaagg
     8821 tcgcaagcgc aagcccatca tagatttcgt gctcggcggg agcggggtgg tactggtgac
     8881 gttctgggtc atcctgctcg acgggcatcc catcgaggcg gcgaggcgct tctacgacaa
     8941 taccctcgcc ttccaggaca acagggtcag tccgtggtcc ctgttcagcc aggttcccca
     9001 actcgcgttc ctgcaacgac cgttggtggc gtttgcgatt ttcctctcca tactcgtcgc
     9061 tttcgtgccg cgcgagaaga cgctgcgccg gctcgcagcg ctctctgcgg cgctcgtcat
     9121 cgccttccag ctcaccgtca actactggtt ctacgcctac atcacctggt tcgagccgtt
     9181 cgtgttcgtc agcctcctgt tggcgacaaa tcagaagtcg gcgttggata gcagtcagcc
     9241 atcagccgtc agcgatcagc cacaagagga ggtcccaacc tgagatgggc gacgccagcg
     9301 agaacgccga acgtcctata ctcaacgtcg agggaaacct cgtggcactc ggtccgctgg
     9361 ggcgggatct cctgccactc tatcagcgtt ggatcaacga cctcggcacg atgcgcactc
     9421 tgggactcgc gccgctcccc atgacgagcg agaaggagca ggactggtat gaccggcagt
     9481 cgaaggcgga agacgacgtg ccgtttacga tctacgagcg agagaccgct cctgaggcgg
     9541 ggtggggtcg ctaccgcctt ctcgtgggcc cacgcaagat gggacaacga atagcgtagc
     9601 cttccgaacg ctactgtcat gcggagaggc gtcagcaaca ctatgtgtgg ttctctagtc
     9661 tctacaggtg catgagggaa ataccgcgag gcattcgaga ggtcgacaga ggagcggacg
     9721 cctcggacgc gcggcattcg gcgggacgaa tctgctgctc atgctgccta tatgttgtag
     9781 tcgagccttt ggtctcacgc ggtcttgaag gcacgctttt cctatcggga cagcgttaca
     9841 caggatgttg tatacggact tcggagcacc ctcatctcgc gcatccgggt gaataagggc
     9901 tcaaggggaa ccgacccagc cgtttggttc ttggcgtcat agactacggt atgcctggat
     9961 gattggttac actcaaagga gggtcgcgtg atcggaagaa tctggcacgg atggacaatc
    10021 ccccagaacg ccgacaagta cgagaggctt ctcaaggaag agatcttctc gggtatagct
    10081 gataagaagg tctctggata caagagaatc cagctcttcc gcaggccgct cgacaacgat
    10141 gaggtagagt tcgttacgat catgtggttt gactcctggg atgcggttaa gcagttcgct
    10201 ggggatgatt atgagcgtgc gtatgtgccc gcggcagcac aggaggtgtt ggcaaggttc
    10261 gatgagcgtt cccaacacta cgagatcagg ga
//
LOCUS       DATMJA010000381         4885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_880871, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000381 DATMJA010000000
VERSION     DATMJA010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880871"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VNT12_09125"
     CDS             complement(<1..602)
                     /locus_tag="VNT12_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWI46321.1"
                     /translation="MRSGSTWGRTRLPIKTSLSFDDQGGFAKAVELCNGSGFCRKRTG
                     GTMCPSYMVTLDEQDTTRARANMLRSVIEGTLPPEELTGQRMKEVMDLCVGCKACKTE
                     CPSRVDVASMKTEVLYQMGREHGFSLRQKAAGHIRRQLALAALIPRLYNTIAGTRLAR
                     RAAGLAGIDTRRSLPRVAKKTFSKRFPGLPQGVGPAEVAL"
     gene            complement(851..2287)
                     /locus_tag="VNT12_09130"
     CDS             complement(851..2287)
                     /locus_tag="VNT12_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWI46322.1"
                     /translation="MSERAPRRSETGAASGHSHATRENILIVLAASMIGLIYGYDLGS
                     IATAILFLTDDFQLTAFQISVVTTAVVVGQLIGAFFAGRITNRFGRKNSMIFVALGYA
                     AFTGLQGLAPNEWFLTGVRFLLGFIIGVSIVTAPAYIAESAPKSVRGSMLVTFQIATV
                     SGICIAYFVGLALSGTESWRLILSLAAIPALIVLFLVLRLPDTARGLMMMGRRAEAVE
                     VLRRVDPDVDPEEEADIIERDLSYEEQGNFSELFRGRFRRAGTFVVGLGFLVQITGIN
                     AIVYYSPTIVQQVGVESSSGAILVTAIIQLFAVAAVVGSFFLVDRLGRRPILLGGITA
                     MGIASAVLIVAFATGPSPVLAIIGILVFTMAFNFGYGALVWAYASESFPARLRTQGGS
                     AMLTSDLAANIVIGIVFLSALGALGGAWTFAIFVVLSVVAFVFVYVLAPETKGRQLED
                     IRGYWYNGGRWPEEGEEATASATGSGRA"
     gene            complement(2523..3485)
                     /locus_tag="VNT12_09135"
     CDS             complement(2523..3485)
                     /locus_tag="VNT12_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWI46323.1"
                     /translation="MNAVGVDVGGTKIAAGVVSHEGELLNEVRYPTANVRERLLSTIA
                     EAIVEVKRGYDVGGVCLAVPGFILARENKVLSAANLEAIEGIPLKEELGGRTGLQVTV
                     ENDANAAAWGEFRFGVGKDVEDLILVSLGTGVGGGVISRGVLLRGARGTGGELGHITV
                     QPAGPRCGCGNRGCLEALASGTAIARRAQKVANERPDSALGRLATERAPLGEDVLDLA
                     RKGDEASVKVLQETGTWLGIGLATFVNIFDPEVIAIGGGVSEAGDLVLEPARRELRLR
                     SHSPSRDLVEIREATLGAKSGMLGAAALARDEDEEYVLGVSATR"
     gene            complement(3690..4685)
                     /locus_tag="VNT12_09140"
     CDS             complement(3690..4685)
                     /locus_tag="VNT12_09140"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HWI46324.1"
                     /translation="MFVQDGVFELSAVGGWSKAFGELHRRIGHRFARSEARERVKRHL
                     VGLLGRVERKNGWQMAEAIGERDPQGVQRLLNLARWDADEVRDDLREYVVEHLGDGET
                     GVLIVDETGFLKKGEKSVGVGRQYTGTAGGTVNCQVGVFLAYSSEKGAAFLDRALYLP
                     RAWTNAPARRDEAGVPAEIVFRNKVELAQEMLERAFEAEVPARWVLADSFYGRSHAFR
                     AWLEERGRPYAVMVPKTNAVPLGGRKKKIERHVERLPRDAFSEVRPARDTGGRRPWEW
                     ACMALAADPKKGMRRWLLVRRSTDDPEDLGFYQAYGPEGTTVEELVKVCQKPPES"
BASE COUNT          901 a         1654 c         1421 g          909 t
ORIGIN      
        1 aagagcgcta cctccgccgg ccccacgccc tgcggcagcc cggggaaacg cttcgagaag
       61 gtctttttgg ctacgcgtgg caacgagcga cgggtatcta tacccgcgag cccggcggcc
      121 ctgcgggcga ggcgcgttcc cgcgatggtg ttgtagagcc gggggatcag cgccgcgagc
      181 gcgagctgac gacggatgtg tcccgccgcc ttctggcgga gtgagaagcc gtgttctctg
      241 cccatctggt agaggacttc ggtcttcatg ctcgcgacgt ccacgcgcga ggggcattct
      301 gtcttgcacg ccttgcatcc gacgcagagg tccatgacct ccttcatgcg ctgtcccgta
      361 agctcctccg gtgggagggt gccttcgatc acggatctga gcatgttggc ccgagccctc
      421 gtggtgtcct gctcgtctag ggtcaccatg tatgaggggc acatcgtgcc ccccgtcctc
      481 ttgcggcaga agccgctccc gttacacaat tcgacagctt tcgcgaagcc gccctggtca
      541 tcgaaggaga ggctcgtctt tatggggagg cgtgtccggc cccacgtcga tcccgaacgc
      601 accgtttatc tgtaggtcga taaagcctgg acagataaac cccgacacct cgcgacgctc
      661 ggacggtagc tctgcggacc acggcgatcg catgacatcg cggatcttgc caccctcgat
      721 caccactgct gtctgaccta acggtacagg tgccagaaca ttaccaacga gggcataagg
      781 tccagtcaga gagaatctcc cttcgctggc atcgtagctc tggtagccct cgtcgacttc
      841 tctatgcccg ctacgcgcgc cccgaacccg tggcgctcgc ggtagcctcc tctccctcct
      901 ccggccagcg gccaccgttg taccagtagc cgcggatgtc ttcgagctgg cgtcccttcg
      961 tttccggggc cagaacgtac acgaagacga acgcgaccac ggacagcaca acgaagatgg
     1021 cgaaggtcca cgctccgccc aacgcgccga gcgcgctgag gaacacgata ccgatgacga
     1081 tgttcgctgc caggtccgag gtcagcatgg ccgaaccgcc ctgtgtgcgg agccgagcgg
     1141 ggaagctttc ggaagcgtag gcccacacga gggcgccgta accaaagttg aaagccatgg
     1201 tgaagaccag gatgccgatg atggccagca caggcgaggg gcccgtggcg aaagcaacga
     1261 tgagtacggc gctagcgatg cccatagcgg tgatcccgcc cagcaggata ggcctgcgcc
     1321 ccaacctgtc gaccaggaag aaggacccca caacggccgc gacagcgaac aactggatga
     1381 tggccgtcac gagtatcgcg ccgcttgagc tctcgacgcc cacctgctgg acaatcgtcg
     1441 gactgtagta gacgattgcg ttgatcccgg tgatctgcac caggaatcca aggcccacga
     1501 cgaaggttcc ggccctccta aagcgcccgc ggaagagctc ggagaagttg ccctgctctt
     1561 cgtagctcag atccctctcg attatgtcgg cttcctcctc aggatctacg tcgggatcga
     1621 cccgccgcag tacctccacc gcctcggccc gccggcccat catcatgagc ccgcgagccg
     1681 tgtccggcag gcgtaggacc aggaagagca cgattagggc cgggatcgcc gcgagcgaga
     1741 ggatcagccg ccaactctcg gtgccggata gcgccaggcc gacgaagtaa gcgatgcata
     1801 tgcccgagac ggtagctatc tggaaggtga ccagcatgga accccggacg ctcttcgggg
     1861 ccgactccgc gatgtacgcc ggagcggtca cgatagagac gccgatgatg aacccgagga
     1921 gaaaccttac gcctgtcagg aaccactcgt tgggagcgag gccttggaga cccgtgaagg
     1981 cggcgtagcc gagagccacg aagatcatgc tgttcttgcg tccgaacctg ttggttatac
     2041 gaccggcgaa gaaggcgccg atcagctgcc cgaccaccac cgcggtggtc accacggaga
     2101 tctgaaatgc ggtgagctgg aagtcgtccg tcaaaaagag gatcgcggtg gcgatggagc
     2161 ccaggtcata gccgtagatc agacctatca ttgaggccgc cagcacgatg agtatgtttt
     2221 ccctcgtcgc atgggaatga ccgctagcag cacctgtctc ggaacggcgt ggcgctctct
     2281 cgctcacgtt tctcctcctc caactccgca ttgtaaagtc ttccaccgaa tgttactaat
     2341 cttacctttg ttcaccctcg gaaggcaacc agagaactcg gcagcaatgt ggagcttgtg
     2401 gagtggctgt ggggcgaatc ctacccgaca ggccgcaaac cgcgatgtcg aagggttccc
     2461 ttcgacacgt cttgatcgcc catcgcctta tcttctcccc ggcagtttcc acgcgacggc
     2521 gcctagcggg ttgcggacac gcccagcacg tattcttcgt cttcgtctcg ggcgagcgcg
     2581 gcggcaccca gcatccccga tttggctccc agagtggcct cccttatctc taccaaatcc
     2641 ctcgaaggcg agtgcgaccg cagccgtagc tccctgcggg caggctctaa caccaaatct
     2701 cctgcctccg agacaccccc acctatcgcg atcacctccg ggtcaaagat gttcacgaaa
     2761 gtcgcgagac ctattccgag ccaggtccct gtctcctgca gcaccttcac agaggcctcg
     2821 tcgcctttgc gcgcaagatc gagcacgtct tccccgagcg gtgctcgctc cgtagcgagc
     2881 ctccccaaag ccgagtccgg tcgttcgttg gctaccttct gagcacgacg tgctatggcc
     2941 gtgccagagg cgagagcctc gaggcatccc cggttgccgc atccgcagcg gggaccggcg
     3001 ggctgtacgg tgatatgccc cagctcccca cctgtccccc gggcgcccct tagcagaaca
     3061 ccgcgcgaga tgactcctcc accgactccc gtccccaggc tcacgaggat caggtcctcg
     3121 acgtccttcc ccacgccgaa gcggaactct ccccaggccg cggcgttggc gtcgttctcg
     3181 accgtcacct gcagtcccgt gcggccaccc agctcttcct tgagaggtat gccctcgata
     3241 gcctcgaggt ttgcagcgct caagaccttg ttttcgcggg ccaggatgaa tccggggaca
     3301 gcaaggcaga cgcctcctac atcgtatcct ctcttgacct ctacgatagc ctcggcgatg
     3361 gtcgagagta gccgttcccg tacgtttgcc gtcgggtatc tcacctcgtt taggagctcg
     3421 ccctcgtgcg agaccacccc ggcggctatc ttcgttcccc ctacgtctac cccgacagca
     3481 ttcacgcttc gcttcaccac cctagtcgac cctgtccatg cgcccctttg acgtcgcgcc
     3541 gcaccccaag cgaacattcc atcaagactt gtagtagctg ttttgctgta ttctacttgc
     3601 agcgtaagca acataataac gtcagccaca agcgaagggg ggtgattatg cgattccaag
     3661 atcgtgtgac catcataacg ggtagcggct caggactcgg gcggtttctg gcacaccttc
     3721 accagttcct ccacggtcgt cccttcggga ccgtaggcct ggtagaagcc caagtcttcg
     3781 gggtcgtcgg tgctcctgcg gaccaggagc cagcgcctca tgcccttctt cgggtccgcc
     3841 gccagggcca tgcaagccca ctcccagggg cgcctgccgc cggtatcccg ggcggggcgc
     3901 acctcggaga acgcatctct tggcaaccgc tcgacgtggc gctctatctt cttcttgcgt
     3961 ccgcctaggg gcacggcgtt ggtcttcggg accatcacgg cgtagggcct gccgcgctcc
     4021 tccagccacg ccctgaaagc gtgcgaacgg ccgtagaacg aatccgcgag cacccacctg
     4081 gcaggcacct cggcctcgaa tgcccgctcg agcatctcct gggcaagctc gaccttgtta
     4141 cggaagacga tctccgcggg gacgccggcc tcgtcccttc tcgcaggggc gttcgtccat
     4201 gccctgggta ggtagagggc ccggtccaag aatgccgcac ccttctcgga ggagtaggcg
     4261 aggaagaccc cgacctggca gttgaccgtg ccgccggccg tgccggtgta ctggcggccc
     4321 accccgacgc tcttttcgcc cttcttcaag aaaccggttt cgtcgacgat gaggacgccc
     4381 gtctccccgt cgccgagatg ctcgaccacg tactccctga ggtcgtcgcg gacctcgtcg
     4441 gcgtcccacc tagccaagtt gagcaggcgc tggacgccct gcggatctcg ctcgccgatg
     4501 gcctcggcca tttgccagcc gttcttgcgc tcgaccctgc ctagcagacc aacgagatgg
     4561 cgcttgacac gctccctggc ctccgagcga gcgaagcggt ggcctatccg acgatgaagt
     4621 tctccgaacg ccttcgacca tccgccgacg gcggacagct cgaagacccc gtcttgcacg
     4681 aacatggcgc tcccttcctc gttccaccgg aagggtagct caggtaccaa ctaagtgatg
     4741 ctgtagagcc ccttcccaga tgttgggcaa ataagaccgg aggcgcacca agaaggcccc
     4801 ttcgccgcgg cggttgccct acgtggcggt cgggaaacgc tctatatagt cgtgttccct
     4861 gactacgagc ccgtccctga tctcg
//
LOCUS       DATMJA010000382         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_643604, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000382 DATMJA010000000
VERSION     DATMJA010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643604"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VNT12_09145"
     CDS             <1..849
                     /locus_tag="VNT12_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HWI46325.1"
                     /translation="GERCGNCDLVTTIANLQLKMGLKVMDDERLRLLTEASNYVSELM
                     NLTPDTHRPYVGRSAFAHKGGLHVDGISKDPATYEHVTPEAVGNVRRTPVGELSGKNS
                     IKTKARELGLEAKDATRILGAIKQREYEGYHYEAADASLALLIGRTTGEDRPLFELET
                     FRIISEKRAGGETTTEATIKLSVGGQRVISTAEGNGPVNALDQALRKAIGPHYPELKE
                     IHLSNYKVRILDEHRATAATTRVLIDSTDGKRMWGAVGVSENIIEASWQALVDGLEYG
                     VHGTKA"
     gene            887..2029
                     /locus_tag="VNT12_09150"
     CDS             887..2029
                     /locus_tag="VNT12_09150"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HWI46326.1"
                     /translation="MSIRTLINPNAREMLDGPYPDRTPLRFHRRLPGYEETPLVDAPK
                     LASALGVGKVFVKDESSRLGLPAFKVLGASWAVYRTLEERLGEDFGDWEEIWELEERL
                     EPLRPLSLLAATDGNHGRAVARVARLLGLGAKIFVPGGMATARREAISDEDAEVIVVD
                     GTYDEAVERSAGEAGERGLVISDMSWPGYERIPSWVIEGYSTMLWEIDDELERRNETG
                     PDLVVVQVGVGAFAAAVARHFRSPGASPRPKLMSVEPASADCLLESVEAGSIVSVPGP
                     HDSIMSGLNCGRLSLVAWPTVSRGIDLLVAVDDEPAHEAMRLAADSGIVSGETGAAGL
                     GGLLEILRTGGAEARRTLGIGRETRVLLFNCEGATDPDAYRRAMVG"
     gene            2212..>2650
                     /locus_tag="VNT12_09155"
     CDS             2212..>2650
                     /locus_tag="VNT12_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46327.1"
                     /translation="MLFSATALLLLAQGLAVLLFLRHGWDALAFPYPLNYGEGPLLDQ
                     SVRLAQFQNIYRTDLTVSPYVVSNYPPLFMLAQVPLVWLFGPALWYGRLISLLSVAAV
                     ALFVGLTLHTLTKDRIAAIAGGLIFPAMPYVVRWSSLSRVDLLG"
BASE COUNT          489 a          817 c          884 g          460 t
ORIGIN      
        1 ggggagcggt gcggcaactg cgacctcgtc acgaccatcg ccaacttgca gctcaagatg
       61 gggctgaaag tcatggatga tgagaggctg cggctgctca ccgaggcttc caactacgtc
      121 tcggagctca tgaacctgac gcctgacacg caccgtcctt acgtggggcg cagcgccttc
      181 gcccacaagg gtgggctaca cgtcgacggt atctccaagg accctgccac ctacgagcac
      241 gtcacccccg aggccgtagg gaacgtaagg cgtacgccgg ttggggaact ctcgggcaag
      301 aactccatca aaaccaaggc aagggagctc gggctcgagg ccaaggatgc aacccggatc
      361 ctcggcgcca taaagcagcg cgagtacgaa ggctaccact acgaggccgc tgacgcctcg
      421 ctcgcgctgc taatagggcg gactaccgga gaggacaggc cgctcttcga actggagacg
      481 ttcaggatca tctccgagaa gcgggccggc ggggagacga cgacggaggc gaccataaag
      541 ctctccgtag gcgggcagag ggtgatctcc accgcagagg gcaatggtcc tgtcaacgcc
      601 ctggaccagg cgctgaggaa agctatcggg ccgcactatc ccgagctgaa agagatccac
      661 ctctccaact acaaggtccg catcctcgac gagcaccgcg ccacggccgc aacaacccgc
      721 gtcctcattg actcgacgga tggcaagcgg atgtggggcg ccgtgggagt ctccgagaac
      781 atcatcgaag cctcctggca agcgcttgta gacggactgg aatacggcgt gcacggcacg
      841 aaggcgtgat cccaagcccg gactccaggt agcattcgaa caggacgtga gcatcaggac
      901 tctcatcaat ccgaacgcca gggagatgct cgatgggcct tacccggatc ggacacccct
      961 gcgattccac cgccggctgc cgggctacga ggagacgcca ctggttgacg ctccgaagct
     1021 cgcctccgcc ctgggcgtcg ggaaggtttt cgtaaaggac gaatcgagcc ggctcggact
     1081 gcccgctttc aaggtccttg gcgcgtcatg ggcggtctac agaaccttgg aggagcgtct
     1141 tggggaggat ttcggggact gggaggaaat ctgggagctc gaggagcgtc tcgaaccctt
     1201 gcggccgctc tcgctactgg ctgccaccga cggcaaccac gggcgagccg tggcgcgggt
     1261 ggcgcgacta ctggggctcg gggcgaagat cttcgtgccc gggggcatgg ccaccgcgcg
     1321 ccgcgaggcc atctcagatg aggatgccga ggtcatcgtg gtcgatggca cttacgatga
     1381 ggccgtcgag cgctctgccg gagaagcagg agagcgcggg ctcgtaatct cggacatgtc
     1441 ctggcccggc tacgagcgca taccttcatg ggtgatcgag ggctactcga cgatgctctg
     1501 ggagatcgat gacgagctgg agagaaggaa cgaaacgggg cccgacctgg tggtcgtcca
     1561 ggttggcgtc ggcgccttcg ccgcagccgt ggcccgacac ttccgcagcc caggagcttc
     1621 gccacgtccg aagctcatga gcgtcgagcc ggcgagcgcg gattgcctgc tcgagtcggt
     1681 ggaggcagga agcatcgtgt ccgtgcccgg gccacacgat tccatcatgt cgggtctaaa
     1741 ttgcggcaga ctctcactgg tcgcctggcc gaccgtgtcc aggggcatcg acctcctcgt
     1801 tgccgttgac gacgagcctg ctcacgaggc tatgcgcctc gcggcagatt cgggcatcgt
     1861 gtccggcgag actggggcgg cgggactcgg cggtctgctg gagatcctgc ggaccggggg
     1921 agcggaggcg cgccggacgc ttggaattgg cagggagacc cgtgtcctgc tcttcaactg
     1981 cgagggcgcc accgaccctg acgcctaccg gagggcaatg gtggggtagc cggacggccc
     2041 ttctcacttc cagcttcgga atggtcggca gagaacgctg ggtgtcgttc gcgcgtagcc
     2101 ggcgccaaaa gcggtaggat acagagcctg agttggagaa cacggaagga cctggcgacg
     2161 agtaagggtt taaagaaagc agcttacacg ggcgggcgga cgcgccgggt cgtgctcttt
     2221 tccgccactg cgctgttgct cctcgcccag ggtcttgcag tgctactctt cttgcggcat
     2281 ggatgggacg cgctcgcttt cccctatccg ctcaactacg gtgaggggcc gctgctcgac
     2341 cagtccgtaa ggctcgcgca gttccagaac atctacagga ccgacctcac cgtctcgccc
     2401 tacgtcgtct ccaattatcc tccgctgttc atgctcgccc aggtgcctct ggtgtggctg
     2461 ttcgggcctg cgctctggta cgggcgtctg atctcgctcc tcagcgtcgc ggctgtggcg
     2521 ctctttgtgg ggctgaccct gcacacgttg accaaagaca ggatcgcggc gatagcgggg
     2581 ggtctgatct tccctgccat gccctacgtc gtgcgctggt catcgctctc tagggtcgac
     2641 ttactggggc
//
LOCUS       DATMJA010000383         3791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_465923, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000383 DATMJA010000000
VERSION     DATMJA010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465923"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..2162)
                     /locus_tag="VNT12_09160"
     CDS             complement(117..2162)
                     /locus_tag="VNT12_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWI46328.1"
                     /translation="MGIAGDIALILVAALLGGVVARWLRLPLILGYIAAGVLVGPNTF
                     GPRVGDVHEIELLAEIGVALLLFTIGLHFSLGELAPVRRIALLGTPVQMALTIAFGYG
                     VGRMMLDLGWVESVWLGALFSLSSTAVVLKTLGEQGVLGTLSARVMIGMLIVQDLAIV
                     VLLTVLPVLENLKEGLPELGLAVLKAAAFIAAMLLLGSRILPMLLARLAAWGSRELFL
                     VSVVAIGVGVGYATYLVGLSFAFGAFVAGMVLSQSDYSHQALAEVEPLRDVFAMIFFV
                     SVGLLIDPSYLWANFGTIALVVVLVFVVKGLIFGGLTRVFGYGNIVPFAVGLGLFQVG
                     EFSFLLAREGISVDAISQETYSLVLATAAVTMALTPLAARLAPVLYGRYRRRFPRELL
                     QTFNLPAGGLQDHAVVAGYGRVGSFVARLLDRLEKPFVVVEANPNRADEAKVAGFPTV
                     YGDAVAIPVLEAAGIGGARLVVLTIPDALAARLAVERVRGLAPKADLLVRAESAAQLE
                     DLGRLGVYEAVQPELEAGLELARQALARFGVAAEEAQTFADGVRRELYAPISGEEGGD
                     RLFARLRQASQAIEVEWVRLSEGQVPEKDEGENLTKPGLLGHTIGDLGVRSETGVSIL
                     AVVRGDEVIPNPGADLALEAGDAVGVLGTPEQRAAFRTIAGANADDSPEIARKTTDR"
     gene            complement(2273..>3791)
                     /locus_tag="VNT12_09165"
     CDS             complement(2273..>3791)
                     /locus_tag="VNT12_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWI46329.1"
                     /translation="RSETVRSGARKAAVEGTFVLPEETGSRLLTEALGDLAEEVEEED
                     GLVLRRTLTGDGRSRCFVGGVSVPVRALASLGEKLVSYHSQREQTRLTEPAEQLAILD
                     DFLDADASEAKHEHGRIWRAVESDRAALQEIKTTSEARLREVEFLRYQVSELESAGYD
                     PQELTDLSRERDRLRNVTDLLQATAAATAALSSEDGTGAVDALGRAASELETASGYDD
                     SLSGLLKRLGGLSAELDDVLYELRSYVDELEADPNRLEAVEDRLAAMRALERKYGDVE
                     GYLQKSRDRLSRLENLDEETAGLEKRLAEGERRLEVIAAALRSGREAAAERLSGKVRE
                     NLEDLNLGKTTFRAELVTTERGPSGTERVEFMIQPNPGEPELPVRRYASGGELSRIML
                     AIRLAQERVEPGATYIFDEVDAGIGGETATAVGAKLRELGDSNQVLTITHLPQIASEA
                     STHVVVEKEETGGRTITRIERIDGEERRRELARMLSGRIDEASLAHASELLVGGK"
BASE COUNT          641 a         1267 c         1212 g          671 t
ORIGIN      
        1 gcaacgtgca cagagtccat gtaggtgata gacgtattat gcgggtttat ctctgtaggc
       61 gcgtaagggc tgtctggagt acattggttg tttcgtgggt ccggcgaggg tggggctcag
      121 cggtcggtcg tttttcgggc gatttcgggg ctgtcgtcgg cgttggcccc ggcgatggtg
      181 cggaaggctg cccgctgctc cggggtacca aggacgccga ccgcgtcgcc ggcttccagc
      241 gccaggtctg cacctgggtt tgggatcacc tcgtcccccc gcacgacggc caggatggac
      301 accccggtct cgctcctcac gccgaggtct cctatggtgt gaccgaggag gccaggcttg
      361 gttagattct ctccctcgtc tttctcgggg acttgtccct cggacagccg tacccactct
      421 acttcgatcg cctgagaggc ttgccgcagc ctcgcgaaga gtctatcccc gccctcctcc
      481 ccggagatgg gggcgtaaag ttcgcgcctt acgccgtcgg cgaaggtctg ggcttcctcc
      541 gccgctaccc cgaatcgcgc gagggcctgg cgcgccaatt ccaggccggc ctccagctcc
      601 ggctgcaccg cctcgtagac gccgaggcgg cccaggtctt ccagctgcgc ggcgctctca
      661 gccctgacca ggagatcggc tttaggggcc aggccccgca cccgttcgac ggcgagtctt
      721 gccgcaaggg cgtcagggat ggtcagcacg acgaggcgcg ctccaccgat cccagcggcc
      781 tccagaacgg gtatggcaac ggcgtcgccg tacacggtgg ggaagcccgc taccttcgcc
      841 tcgtcggcgc ggttcggatt tgcctccacg acgacgaatg gcttctcgag cctgtctagt
      901 agcctggcga cgaaggagcc aacccgaccg tacccggcga cgacggcgtg gtcctgaagc
      961 cctcctgccg gcaggttgaa ggtctgcagg agctctctgg gaaaccgccg ccggtaccga
     1021 ccgtagagga ccggcgcgag ccgtgccgcc aggggggtaa gggccatcgt gacggccgcg
     1081 gtggccagca cgagcgagta ggtctcctgg gagatggcat ctacgctgat cccctccctg
     1141 gcgagcaaga acgagaactc cccgacctga aagagcccca atcctactgc gaaggggaca
     1201 atgttgccgt agccgaagac cctggttaga ccaccgaaga tcaggccctt cacgacgaag
     1261 accagcacca cgacgagcgc gatggtcccg aagttcgccc agagatagga ggggtcgatc
     1321 agtagcccca cggagacgaa gaagatcatg gcgaacacgt cgcgtaaggg ctctacctcg
     1381 gccagggcct ggtgactgta gtccgattgg gagaggacca tccccgctac gaacgccccg
     1441 aaggcgaaag aaagccctac gaggtaagtc gcgtacccga cgccgacgcc tatggcgacc
     1501 acggagacga ggaagagttc gcgcgagccc caagccgcca gccgcgccag gagcataggt
     1561 agtattcgtg agcccaacag gagcatggcg gcgatgaacg ccgccgcctt gaggactgcc
     1621 aggccgagtt cgggaaggcc ttcctttagg ttctccagta ctggcaacac ggtcaggagc
     1681 actacgatag ccaggtcctg gacgatgagc atcccgatca ttaccctcgc cgacagggtg
     1741 ccgaggaccc cctgctcgcc caaggtcttg aggactacag cggtgctcga tagcgagaac
     1801 agggccccca gccacaccga ctcgacccac cccagatcca acatcatacg gccaaccccg
     1861 tagccgaaag cgatggtgag cgccatttgg acaggggtgc ccagcaaggc gatgcgccgc
     1921 acgggcgcga gctcccccag ggagaagtga agtccgatcg tgaacaggag gagcgccacc
     1981 ccgatctctg ccaacagctc tatctcgtgt acgtcaccga ccctcggccc gaaggtgttc
     2041 ggaccgacga ggacgccggc tgcaatgtag ccgaggatca ggggcagccg tagccatcgg
     2101 gccacgacac ctcccaggag ggcggccacc aggatgagcg ctatgtcacc ggcgatcccc
     2161 aaatcctggg cctcacttta ccaccgtcga agagcggcac cgtccggcgg cgatgggctg
     2221 gccggtggtt cgggcgcgcc gccattcccc ggccttccgg gccggaggat ccctatttcc
     2281 cgccaaccag gagttcggag gcgtgggcca gggaggcttc atcgatcctt ccagagagca
     2341 tcctggcgag ctccctgcgg cgctcctcgc catcgattcg ctctatgcgc gtgatcgtcc
     2401 gccctcccgt ttcttctttc tcgacgacga cgtgcgtgct tgcctcggag gctatctgag
     2461 ggaggtgggt gatggtcagg acctggttgc tatcgccgag ttccctcagc ttcgcgccca
     2521 ccgccgtcgc tgtctcgccg cctataccgg cgtcgacctc gtcgaagatg tacgtcgcac
     2581 cgggctccac gcgctcctgg gcgagcctta tggccagcat tatcctggag agctcccctc
     2641 ctgatgcgta gcgcctcacg ggcagttcgg gctcacccgg gttgggttgg atcatgaact
     2701 ccacccgctc cgtgcctgac gggccacgtt ctgtggtcac gagctcggcg cggaaggtcg
     2761 tcttcccgag gttgaggtct tcgaggttct ctcgaacttt ccccgacaac cgctctgcgg
     2821 cggcctccct gcccgagcgc agcgcggccg cgatgacttc gaggcgccgc tcgccttccg
     2881 cgagtctctt ttcgagcccg gcggtctcct catccaggtt ctcgaggcgt gagagacggt
     2941 cgcgcgattt ctggaggtat ccctcgacgt ccccatactt gcgctccagg gcccgcatgg
     3001 cggcgaggcg gtcctcgacg gcctccaacc ggtttgggtc agcctcgagt tcgtccacgt
     3061 aggaccttag ctcgtagagg acgtcatcca gctccgcgga gagcccgccc agtcgcttca
     3121 ggagcccact caggctgtcg tcgtaccccg aagccgtctc cagctctgat gcggcgcgcc
     3181 cgagggcgtc tacggctccc gtgccgtctt cggaggagag ggcagccgtt gcggctgccg
     3241 tggcctggag aaggtccgtc acgttcctga gacggtcgcg ttcacgggag agatccgtga
     3301 gctcctgcgg gtcgtagccc gcagactcca gctcagagac ctggtagcgg aggaactcta
     3361 cttcccgcag ccgcgcctcg gaggtagtct ttatctcctg aagcgcggcg cgatcgctct
     3421 ccacggcgcg ccagatccgt ccatgctcgt gtttcgcctc ggaggcgtcg gcgtccagaa
     3481 agtcgtccag gatagcgagt tgttcggccg gctcggttag acgtgtctgc tcgcgctggc
     3541 tgtggtagga gaccagtttt tctccaagag aagcgagcgc ccttacgggg accgagacac
     3601 cgccaacaaa gcacctggag cggccatctc ctgtcagcgt ccgccgcagg acgaggccat
     3661 cctcctcttc cacttcttcc gctaggtcgc cgagggcttc cgtgaggagc cgagatcccg
     3721 tctcttcagg gaggacgaaa gtgccttcga cggccgcctt gcgtgcgccc gagcggaccg
     3781 tctcggagcg g
//
LOCUS       DATMJA010000384         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_406914, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000384 DATMJA010000000
VERSION     DATMJA010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406914"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1002)
                     /locus_tag="VNT12_09170"
     CDS             complement(160..1002)
                     /locus_tag="VNT12_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46330.1"
                     /translation="MEGSNDRGLRPDEVEMILRRASELNARRWTRNSSDTPSVSPEVL
                     VKVAAAAGIPEQDVRRAMWDMASERTAEPRSFARKFYGPARLRAVRWVERPAESTREY
                     LEGLLRLQQGLKLRRKTEVGSLWDPGDELGVVRRTLDFSGDTTLLKARLVELRVESLG
                     EERCEANLTADLSNQRGEYLSLAGILGATLAALFMLAGMQSPLFFLGVLPVLAASALG
                     FRFAYSRASQELRRALDNLLDAAEESQPQEEPQDRGDRPRGQIQGLEPIPRFSPDRSK
                     RVEP"
     gene            complement(1025..1594)
                     /gene="pgsA"
                     /locus_tag="VNT12_09175"
     CDS             complement(1025..1594)
                     /gene="pgsA"
                     /locus_tag="VNT12_09175"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWI46331.1"
                     /translation="MSLANQLTLIRLVAVVPVMGALYLPFEWARPVAVVLYAAAILTD
                     YFDGIIARRNNEVTGFGKLMDSIADKALIVSLFFALVGEGSMAAWMAAIMVIREFAVT
                     GLRMVALEAGEVIAANRWGKAKMNAQSLAVFILLLGKPGAAGWQGVVSQIGWWVMLVA
                     VVLTILSGWSYLKDTPRILSITSKRDQRL"
     gene            complement(1687..>2008)
                     /locus_tag="VNT12_09180"
     CDS             complement(1687..>2008)
                     /locus_tag="VNT12_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI46332.1"
                     /translation="VPPTAQAAIQQGHLVARNVLKTIDGKGDLGEFEYKPLGQLVELG
                     SDFAVNEVMGVRFTGMIAAVFWRMAYLVRLNSPQSKVRVAADWIVGLFLRPAVTQIRS
                     SYEE"
BASE COUNT          379 a          663 c          606 g          360 t
ORIGIN      
        1 ctctacttcc acaaagagga gattcccggc cctccagacc aaagcgattt acagtggcat
       61 ccacctgctg cgaatcgcgc atccgcctcc gagcggaaac aacaagctga cttgctgaag
      121 tgctgagtag cgcccgaaag ggtgcgtgct gaaatgctct cagggttcta cgcgcttgct
      181 cctgtcaggg gagaaccttg gaatgggttc gaggccctga atctggccgc ggggacgatc
      241 gccgcggtcc tgaggttcct cttgaggctg gctctcttcg gcggcgtcga ggaggttgtc
      301 gagggcacgt ctcagctcct gggaagccct actgtaggcg aacctgaacc cgagtgccga
      361 cgccgcgagc acgggcagga cgccgagaaa gaagagcggg ctctgcatcc cggcgagcat
      421 gaacagtgcg gccagcgtgg cccccaggat gccggcgagc gagaggtact caccgcgctg
      481 gttggagagg tcggccgtca ggttggcctc gcatcgctct tcgcccaaac tctcgacccg
      541 cagttcgacc aacctggcct tgagcagtgt cgtgtccccc gagaagtcca gcgtccttcg
      601 cacgaccccg agctcgtccc cagggtccca caaagagccg acctccgttt tgcgcctgag
      661 cttgaggccc tgctggaggc gcaacaaccc ttcgagatac tcgcgggtac tctcggcggg
      721 acgctctacc caccgcaccg cccgcagacg tgcaggaccg tagaacttgc gggcgaacga
      781 gcgtggttcc gccgtccgct ccgaagccat gtcccacatc gcccggcgga cgtcctgttc
      841 cgggatcccg gccgcagcgg ccacctttac gagaacctcg ggagagacgg aaggggtgtc
      901 ggaagaattc cgcgtccacc tgcgagcgtt cagttctgaa gcgcggcgca ggatcatttc
      961 gacctcgtcc ggcctcaggc ccctgtcatt gctgccctcc atatggggcg tgattctaca
     1021 gcttttagag cctctgatct cgcttggagg taatcgacag gattcgcggg gtgtccttca
     1081 aataggacca gcccgagagt atggtaagca ctacggcgac gagcatcacc caccacccga
     1141 tctgggagac gactccctgc cagccggccg cccctggctt gcccagaagg agtatgaaaa
     1201 cggcgaggct ctgggcgttc atcttcgcct tgccccagcg gttcgcggcg atgacctccc
     1261 ccgcctcgag ggcgaccatc ctgagccccg tcacggcgaa ctcccggata accatgatcg
     1321 cggccatcca tgcggccatc gagccctccc ctacgagcgc gaagaagagt gagacgatga
     1381 gggccttgtc ggctatggag tccatcagct tcccgaagcc cgtcacttcg ttgttgcggc
     1441 gggcgataat cccgtcgaag tagtcggtca ggatcgcagc cgcgtaaaga acgacggcga
     1501 cgggacgcgc ccactcgaag ggcaggtaca gcgcgcccat cacgggaacg acagcgacga
     1561 gccggatcag ggtgagctgg ttagcgaggc tcaaacgacc cccgtctttt atgccatgtc
     1621 acctgcatct tctcctctag ccacattcaa cgtagcacct ttgtggccgc gtctcaaggg
     1681 ctcccgctat tcctcgtagg agctccgaat ctgggtgacg gctggccgca ggaacaggcc
     1741 gactatccag tccgcagcga cccgcacctt gctctggggg ctgttcaggc gcaccagata
     1801 ggccattcgc cagaaaacgg ccgcgatcat cccggtgaac cttacgccca tcacctcgtt
     1861 gacggcgaaa tcgcttccca gctcgacgag ctggccgagc ggcttgtatt cgaactctcc
     1921 cagatcaccc ttaccatcga tggtcttgag cacgttacgg gcgaccaggt gcccctgctg
     1981 gatcgcggcc tgcgcggtcg gcggaacg
//
LOCUS       DATMJA010000385         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_170325, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000385 DATMJA010000000
VERSION     DATMJA010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170325"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VNT12_09185"
     CDS             complement(<1..527)
                     /locus_tag="VNT12_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWI46333.1"
                     /translation="MPESLLDLTAYGLAREIEAGEVSASEAAEIANARVDEVEDEINA
                     FVTITPGLALERAEAVDARNGERALWEGVPLVVKDVLSTRGVRTTCGSRILENFEPLY
                     DASALLNFGENLIMLGKANCDEFAMGSSTENSAWGPSRNPWDLGRVPGGSSGGSAAAV
                     AAGEGWWALGTDTGGS"
     gene            complement(529..810)
                     /gene="gatC"
                     /locus_tag="VNT12_09190"
     CDS             complement(529..810)
                     /gene="gatC"
                     /locus_tag="VNT12_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWI46334.1"
                     /translation="MISEEQVRHVANLARLGLTDDEIEKIGGQLDAILDSIEKIQELE
                     LADVPPTANPLDLSNVLRSDEPRPELAQEEALTPAPERVDDLFAVPRID"
     gene            1123..>2026
                     /locus_tag="VNT12_09195"
     CDS             1123..>2026
                     /locus_tag="VNT12_09195"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HWI46335.1"
                     /translation="MTIHEAASESDRTGAGGAPGYQPIENYGIIGDLHTTALVGMDGS
                     IDWLCLPHHDSPSVFAAILDSAKGGRFKVSPVGGEVTTKQLYWPDTNVLVTRFFTPDG
                     VGEITDYMPIAALESGHKRHQIVRRVEVVRGTMTFRMECSPAFDYARKEHETRIVPEG
                     ATFHSLGLSLGLASSFPLEQRDNGTTAEFTLQEEQSTVFVLREIEAGEECGVCLSKMD
                     EEEIFMQTVQYWRRWLSKCTYTGRWREMVRRSALTLKLLTFEPTGAIVAAPTMGLPED
                     IGGERNWDYRYTWLRDAAFTLYGLL"
BASE COUNT          398 a          656 c          616 g          356 t
ORIGIN      
        1 gagccgcccg tgtccgtccc gagcgcccac cagccctcgc ccgccgcgac cgcagcagca
       61 gatccgcccg aggatccccc gggaacacgg cccaggtccc aggggttgcg actgggaccc
      121 caggccgagt tctctgtcga tgagcccatg gcgaactcgt cgcagttggc cttgcctaac
      181 atgatgaggt tctcgccgaa gttcagtagc gccgatgcgt cgtagagtgg ctcgaagttc
      241 tcgaggatcc tcgacccgca agtcgtcctc acgccacgcg tagaaagcac gtccttgacg
      301 acgagtggga ctccctccca gagagcgcgc tcgccgttcc tggcgtctac cgcctcggcg
      361 cgctcgagcg ccagcccggg ggtaatcgta acgaaggcgt tgatctcgtc ctcgacctca
      421 tcgacgcgcg cgttggcgat ctcagcggcc tcgctggccg agacttcgcc ggcttctatc
      481 tctcttgcga gcccgtaggc cgtgaggtcg agcagacttt ccggcatctc agtcgatcct
      541 ggggacggcg aagaggtcgt cgacacgctc gggggcaggg gtgagggcct cttcttgcgc
      601 gagctcggga cgcggctcgt cagaacggag gacgttcgag aggtcgagcg ggttcgccgt
      661 cggcggcacg tcggcgagct cgagctcctg gatcttctca atgctgtcca gaatcgcgtc
      721 gagctgcccg cctatcttct ctatctcatc gtccgtaagc cccaaccgcg ccagattcgc
      781 aacgtggcgc acctgctctt cggatatcat gccaggcctc cggtctttcg cttgtagaat
      841 cagaacttat cttagcgcag cgcctgcaag ccctcccacc agcaagcctg cgagcgccac
      901 atccttcatc tgaagccacc actgttgcac gcgcgggcca acggggttct aattgtacag
      961 gcgcccacag atatcttccc gatggaagat tgactcaccc aggagccgag agtatggccg
     1021 atcagagact ctatacaagg gaaagggctc atgaggtgac cggatgacgg aagacgacaa
     1081 gacacgatcg cgagaccccg ctgcggagga ggaagcaagg agatgacgat ccacgaggca
     1141 gcaagcgaat ccgaccgcac tggtgcaggg ggcgcgccgg gataccagcc catagagaac
     1201 tacgggatca tcggcgatct gcacaccacg gccctggtgg ggatggacgg ttccatagac
     1261 tggctgtgcc tgccgcacca cgactcccca agcgtcttcg cggccatcct cgacagcgcg
     1321 aaaggcgggc ggttcaaggt ctcaccagta gggggcgagg tcaccaccaa acaactctac
     1381 tggcctgata ccaacgtcct ggtcacgcgg ttcttcacgc ccgacggggt tggagagatc
     1441 acggactaca tgccgatagc cgctctggag agtggccaca agcgccacca gatcgtccgt
     1501 cgcgtcgagg tggtgcgcgg caccatgacg ttccggatgg agtgttcccc tgccttcgat
     1561 tacgcccgca aagagcacga gaccaggatc gtccccgagg gagcgacctt ccactccctt
     1621 ggtctcagcc tgggcctggc atcatccttc cccctggagc aaagggacaa cgggaccacc
     1681 gccgagttca cgctgcaaga ggaacagtcc acggttttcg tgctcaggga gatagaagcc
     1741 ggagaagagt gcggggtctg tctttctaaa atggacgaag aagagatctt catgcagacc
     1801 gtccagtact ggcgccgctg gctttccaag tgcacctaca ccggtcgctg gcgcgagatg
     1861 gtccgccgct cggcccttac cctgaagttg ctcaccttcg agccgacagg agcgatagta
     1921 gcagccccta ccatgggatt gcccgaggac atcggcggag agcgaaactg ggactaccgc
     1981 tacacctggc tcagggacgc ggcatttacc ctctacgggc tcctgc
//
LOCUS       DATMJA010000386         3742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_407064, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000386 DATMJA010000000
VERSION     DATMJA010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407064"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VNT12_09200"
     CDS             <1..412
                     /locus_tag="VNT12_09200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI46336.1"
                     /translation="GITSTASRWWAGRFGDRSDPRLLLAPGLLACAAGMVCLPLGGVV
                     LLIGAVLFGTGFGLLQNATLILMMGRVSKSEYGLGSTLWNAAFDTGTGIGAFSFGFVI
                     SAVGFSWSFYICSALVASALLLVLLDYSSTQDRP"
     gene            646..1131
                     /locus_tag="VNT12_09205"
     CDS             646..1131
                     /locus_tag="VNT12_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46337.1"
                     /translation="MYGQGIMGYAGGRRTMAPYGRPFPPRFMMGLVIGGLFLLFVAGP
                     AFHFFPLLFLLVWAVPFFLAPAFVTVARGLSGLSEARARKGGAVAEESKERELLEALA
                     RHGEISPALAALETSLTVAEANRMLSDLANSGHVEVRAREGRLGYALWERDRRELTEG
                     A"
     gene            1160..1726
                     /locus_tag="VNT12_09210"
     CDS             1160..1726
                     /locus_tag="VNT12_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWI46338.1"
                     /translation="MFGPKAMKGFFGGWHGRGFGHGPRRGRMFEKGDLKYVILDLLKD
                     EPSHGYEVIRKLEERSRGFYSPSPGSVYPTLQLLEDMEYVSATQRDGKKVYAITDQGR
                     KFLDENRRSVQDIWGRFGGSWDPEVASELHEIRHDVMDLGKLFGQKMREGSVDREKLA
                     RIRSVISAATREIEDILREREDPGTTSV"
     gene            1929..2654
                     /gene="pcaH"
                     /locus_tag="VNT12_09215"
     CDS             1929..2654
                     /gene="pcaH"
                     /locus_tag="VNT12_09215"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HWI46339.1"
                     /translation="MAQRKGEEAAGYGREYEGVQPPYLYPDYVATRLRAPKKPLIILP
                     GTLSDTTGPAYGRGAVGELDDDLTRQHSGEPLGERIIVTGRVLDGDGRPVRNSLVEIW
                     QANAAGRYTHHLDQHPAPLDPNFSGAGRCLTDGEGRYRFVTVKPGAYPWKNHDNAWRP
                     AHIHFSLFGTAFRSRLITQMYFPGDPLLEHDPIFQSVRDRKARQRMISSFDLQTTRPE
                     WALGYRFDIVLEGSEATPMENHR"
     gene            2651..3217
                     /gene="pcaG"
                     /locus_tag="VNT12_09220"
     CDS             2651..3217
                     /gene="pcaG"
                     /locus_tag="VNT12_09220"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HWI46340.1"
                     /translation="MSLEPTPSQTIGPFFHEALLDRNHTELVSLDHPEGIRIDGTVYD
                     GEGEVVPDAMVEIWQANRAGRYNHPADNREDLSLDEDFLGFGRSGTDRGGTFSFLTLK
                     PGPVAGPGGELQAPHVMVSVFARGLLKRLVTRIYFPDEQEANAVDPVLSSIEDQELRR
                     TLVARDEEDALRFDIHLQGEGQTAFFEF"
     gene            3220..>3742
                     /locus_tag="VNT12_09225"
     CDS             3220..>3742
                     /locus_tag="VNT12_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWI46341.1"
                     /translation="MAEELFRPIFVPERFSKAVSGRAWIGAMLEAEGALAVAQARAGL
                     IPHQAAEIIVSCCDADRFVPEEIGRKGHAQGNPVPPLVHSLTRAVTRVSEDAAGHVHK
                     GATSQDIMDTAAMLVCRNALGLVLAEIDGISAACARLANTYRDTITPGRTLLQQALPT
                     TFGKKVAGWLVSVL"
BASE COUNT          724 a         1106 c         1209 g          703 t
ORIGIN      
        1 cggcatcacc tccaccgcca gccgctggtg ggccggaagg ttcggcgacc gcagcgaccc
       61 gcgcctcctg ctcgcccccg gtctcctcgc ctgcgcggcg gggatggtct gccttccgct
      121 ggggggtgtt gtcctgctaa tcggggccgt gctattcggg acagggttcg ggttgctcca
      181 gaatgcaacc ctcatcctga tgatggggcg tgtctcgaaa tccgagtacg ggctcggcag
      241 cacactgtgg aacgcggctt tcgatacagg gaccgggata ggagccttct cgttcgggtt
      301 cgtgatctcg gctgtcgggt tctcctggtc tttctacatt tgctcagcgc tggtggcatc
      361 ggccctcctg ctcgtgctcc tcgactattc atcgacacag gaccgaccgt aaccatcgcg
      421 aactccgcga gaaatacctc ggaggacgcc gcctcagcag tacgatcgtg gcgccgtaga
      481 atcatcggat ttctctcagc attattccag ccacgcgtcg cagacttcat gagacggtca
      541 ccctcaatcg gtcccaatat ccctgttcca ccatacaaag ttctatcaag atatatcgtg
      601 atatactgca aaaccacagt ggaccgtcgg gatgaaggag gcgagatgta tggtcagggc
      661 ataatgggct acgcgggtgg gaggcggacg atggcacctt acgggaggcc ctttcctccg
      721 aggttcatga tggggttggt aataggaggg ttgtttctgc tcttcgtggc tggtcccgcc
      781 ttccacttct tcccgcttct tttcctactc gtctgggccg ttcccttctt cctggcgccg
      841 gcgttcgtga ccgtcgcgcg gggcttgtcg gggctctcgg aggcccgtgc gcggaaaggt
      901 ggggctgtag cggaggagag caaggagcgg gagcttctgg aggcgctcgc gcggcatggg
      961 gagatctcgc ctgcgctggc ggcgctggag acttcgctca ccgtcgccga ggcgaaccgg
     1021 atgctctcgg atcttgccaa cagcggtcac gtggaggtaa gggcgcgcga gggaaggttg
     1081 gggtacgcgc tctgggagcg ggaccggcgt gagttgacgg aaggggctta gtgtgcgttt
     1141 gcgaaaggag gtcgagaaca tgttcggtcc aaaagcgatg aaagggttct tcggagggtg
     1201 gcacgggcgg gggttcggcc acggtccgcg gcggggacgg atgttcgaga agggggactt
     1261 gaagtacgtg atcctcgacc tgctcaagga cgagccctcc cacggctacg aggttatacg
     1321 gaagctggag gagaggtccc gcgggttcta ctctccgagt cccggctccg tttatcccac
     1381 actgcaactg ctcgaagaca tggagtacgt gagcgccacc cagcgagacg gcaagaaagt
     1441 ctacgccatc acggaccaag ggcggaagtt cctggatgag aaccggcgct ccgtccagga
     1501 tatctggggc aggttcggag gaagctggga ccccgaggtc gcatccgagc tacatgagat
     1561 caggcacgac gtcatggatc tcggcaagct cttcggccag aagatgcgcg agggaagcgt
     1621 agacagggag aagctggccc gcataaggtc tgtgatctca gccgccacaa gagagataga
     1681 agatatcctg cgggagcgcg aagatccagg gacgacaagc gtgtgaaaca gcatttcagc
     1741 gcgctccggc ttcgcctccg cttgtcagca cggcggctgc gccgccttgt cagcctgtca
     1801 gctagggctt ttaggcacac gtgagctgaa gtgctgacaa gtcgcctgga acaggcgacg
     1861 tgctgaaatg ctgaaatgct gatattttga ggcaggtgta gagcgggacg agcaaagggg
     1921 aacgtcgaat ggcacagcgg aagggagaag aagccgctgg gtacgggcgc gagtacgagg
     1981 gcgtccaacc cccttacctc tatcctgact acgtggcgac gcggctcagg gcgccgaaga
     2041 agccgctcat catcctccct gggacactct cggacaccac tggtccggca tacgggcgcg
     2101 gcgctgtagg ggagcttgac gacgatctga cccgccagca ttccggcgag cctctggggg
     2161 agaggatcat cgttacagga cgcgtcctcg acggcgacgg acgcccggta cgtaacagcc
     2221 tcgtcgagat ctggcaggcc aacgccgcag gacgctacac ccaccatctg gaccagcacc
     2281 ctgctccttt ggacccgaat ttctcggggg cggggaggtg cctcaccgac ggcgagggtc
     2341 ggtacaggtt cgtgacggta aagcctgggg cctatccctg gaagaaccat gacaacgcct
     2401 ggcgcccggc gcacatacac ttctcgctgt ttgggacggc gttcaggagc cgcctcataa
     2461 cccagatgta cttccctggc gacccgcttc tagagcacga tcctatcttc cagtcggttc
     2521 gggaccggaa ggctcgacag cgcatgatct cgagcttcga cctccaaacg acacggcctg
     2581 agtgggctct tggctaccgc ttcgacatcg tgctcgaagg aagtgaagcc accccgatgg
     2641 agaatcacag atgagcctcg agccaacacc ttcccagacg ataggaccct tcttccacga
     2701 ggcgctcctc gacaggaacc acaccgagct cgtctcgctc gaccaccctg aaggtataag
     2761 gatcgatgga acagtctatg acggggaagg cgaggtcgtg ccggacgcga tggtcgagat
     2821 ctggcaggct aacagggcgg gccgctacaa tcatcctgcg gacaatcgag aggacctatc
     2881 cctcgacgaa gatttcttgg gcttcgggcg ctcgggcaca gatagaggcg gcacgttctc
     2941 cttcttgacg ctcaagccag ggcccgttgc cggtccgggc ggagagcttc aggcaccgca
     3001 tgttatggtc tccgtgtttg cccgcggtct cctgaagcgc ctggtgaccc gcatctactt
     3061 cccagacgag caagaggcca acgctgtcga ccctgttctt tcgtccatcg aggaccagga
     3121 gctccggagg acccttgtag cccgtgacga ggaggatgct ctgcgcttcg acatccactt
     3181 gcagggcgaa gggcagaccg cgttcttcga gttctagaga tggctgagga gctgttcagg
     3241 ccgatcttcg taccggagag gttttcgaag gcagtctctg gaagagcctg gatcggggcg
     3301 atgctcgagg ctgagggcgc tctagcggtt gcgcaggcga gggctggtct gatccctcac
     3361 caggcagcgg agatcatcgt ctcctgctgc gacgcggatc gctttgttcc cgaggagata
     3421 ggacgcaaag gacacgccca gggcaacccc gtccccccgc tcgtccactc tctcaccaga
     3481 gcggtcacaa gggtctccga agacgcagcc ggccacgtcc acaagggggc tacgagccag
     3541 gacatcatgg acacagccgc tatgctcgtg tgccggaatg cccttggcct agtactagcc
     3601 gagatagatg ggatctcggc agcctgcgcc cgactggcca atacttacag agacaccata
     3661 acgccaggcc gcacgctgct ccagcaggcg ctcccgacga ccttcggcaa gaaggttgcc
     3721 ggctggctgg tctcggtgct cg
//
LOCUS       DATMJA010000387         4200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_289081, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000387 DATMJA010000000
VERSION     DATMJA010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289081"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VNT12_09230"
     CDS             <1..659
                     /locus_tag="VNT12_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46342.1"
                     /translation="AQHLKSWTDRTGHVVPVRMLHEFNYPPLSKGWGGPDYMPRFWRR
                     WATILRGGDVDGNLRAMGQPALRSSQSVGRNDNLLLVWCILAASAGATAETNWWDYWP
                     GGQWVDICGADMYPSPDDDDRRGQFAMLNRFNDFARKHSKPLCIPEFNWAARKVISLP
                     GPTREFLDWIVRHAKRVRFTAQFDTTKDFAVVRRGDPHPELRKLYSNYYNKSAFGGDR
                     "
     gene            1068..1340
                     /locus_tag="VNT12_09235"
     CDS             1068..1340
                     /locus_tag="VNT12_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46343.1"
                     /translation="MRQGFLFEHAGEPLSRYAVEYSATTGKPKGIARPVLFGTAIALL
                     QPRHFGLEVLGEGGWLKTLRLADYAPRKARPGSLQQGLFPYHEAWG"
     gene            complement(1394..2281)
                     /locus_tag="VNT12_09240"
     CDS             complement(1394..2281)
                     /locus_tag="VNT12_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWI46344.1"
                     /translation="MFSGVKLNKARRDHEHEDREAVLPGALKGYVYRPQGWLSPVVLV
                     DGRMEGVWRHERRGDRLSVEIEPFAHQPDWVRRAEEEAERLARFVGGELELSGRTREE
                     ERQSPAGAEEPRTGLVPASERRTNPAVFTASGSATINGRHCTLKKRNAMAERPDAANP
                     ATGSVRYVDPEGLNKNPAFTNVVVVEGPARTVYVGGQDSVDASGNIVGKGDFAAQTEQ
                     VLANVRAALAAGGAGPEHVIKWNLLVVEGLSLQEGFSAFQRAWVATPNPPAITMAYVA
                     GLANPEFLVEMDAIAVVPL"
     gene            complement(2305..3219)
                     /locus_tag="VNT12_09245"
     CDS             complement(2305..3219)
                     /locus_tag="VNT12_09245"
                     /inference="COORDINATES: protein motif:HMM:NF006159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI46345.1"
                     /translation="MVPKLKGKVALVAGGTRGAGRGIAVELGAAGATVYVTGRSTRDG
                     RSEYDRPETIEETAELVDRAGGRGSAVRVDHLEPEQVRTLVGRIGDEQGRLDVLVNDV
                     WGAERLFEWNVPVWEHSLERGLRMLRLAIDTHIITSHFALPLLIKNEGGLVVEMTDGT
                     AEYNENNYRVSLFYDLAKTSVIRMAWTQAQELAPHGCTAVALTPGWLRSEMMLENFGV
                     EESNWREATAIQPHFVISETPRYVGRAVAHLAADPEVARWNGRSLSSGALAKEYGFTD
                     LDGSRPDAWRYLVEVQDAGLPADTTGYR"
     gene            complement(3524..3850)
                     /locus_tag="VNT12_09250"
                     /pseudo
     CDS             complement(3524..3850)
                     /locus_tag="VNT12_09250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
     gene            3963..>4200
                     /locus_tag="VNT12_09255"
     CDS             3963..>4200
                     /locus_tag="VNT12_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46346.1"
                     /translation="MLEYRTGRNEPRCVATEDAVGRGHNEGGGDTLISDVAGDERELA
                     VGQLKEIVKIAADSTRRLVVGCEAPSREGGQFLRE"
BASE COUNT          713 a         1355 c         1318 g          814 t
ORIGIN      
        1 ttgcgcaaca tctgaagtct tggaccgata ggacgggcca tgtcgtcccg gtgaggatgt
       61 tgcatgagtt caactaccca cccttgagta agggttgggg tggtcctgac tacatgccga
      121 gattctggcg gcgttgggcg accatcctta ggggtgggga cgtggatggc aatctgaggg
      181 ccatggggca gcccgcgctt cgtagctctc agagcgtggg acgcaacgac aacttgctgt
      241 tggtgtggtg catcctggca gcctctgcag gcgctacggc agaaaccaac tggtgggact
      301 actggccagg agggcagtgg gtggatatct gcggcgctga catgtacccc tccccagacg
      361 acgacgaccg aagggggcag tttgccatgc tcaaccgctt caacgacttc gccaggaaac
      421 acagcaagcc gctgtgcatt cctgagttca attgggcagc gcgcaaagta atcagcctgc
      481 cgggtcctac tagagaattt ctcgactgga tcgtaagaca tgccaagcgc gtgcggttta
      541 ccgcccagtt cgataccact aaggacttcg ccgtggtcag gaggggggat ccgcatcccg
      601 agctaagaaa gctttactcg aactactaca acaaatccgc ctttggggga gatcgctgac
      661 acgagtgtct acagcgacca cggcctggcc agagcacgac gcgcccgaag agctcttccc
      721 tctccctggc ccatcccgcg tgcttctcac tcatgaacca tcatcctcct agcactctcc
      781 cccaccacga cctgcgtgca ccctcctccc cccctccagc agcgggactg ggctccgcct
      841 cctccggggc cgtctccgtg gattctcgcg actctgaggc cgttttgtgc gcttggtggg
      901 gtaccgcttc gacgcctgtc tggagttttc atacagtctt gtaaggtgaa ttctcaggag
      961 ttcaacttcg cgttgacgga gttctccgaa gttgaacttc cgctttacgg agttctccga
     1021 agttcgtctt ctgggcgttc tcggaagtac cgcatagtcc cggttctatg cggcagggtt
     1081 tcttattcga gcacgccggg gagccgctca gccgctacgc cgtggagtac tctgccacaa
     1141 caggaaaacc caaggggatc gctcgtccgg tgctcttcgg gacggcgatc gcgttgctcc
     1201 agccgcggca ctttgggctt gaggtcctcg gcgaaggcgg atggctcaag acgctgaggc
     1261 tggcggatta cgccccgagg aaggcgcgtc cgggatcgct tcagcagggc ctgttcccct
     1321 accacgaggc ctggggctga gccccgatcg gaggtcgggg cgtgcccttc gaggccgcga
     1381 gccgcggcgg ggctcacaga ggcacgacgg cgatggcgtc catctccacc aggaactccg
     1441 ggttcgccaa ccccgcgacg taagccatgg tgatcgccgg cgggttcggc gtggcgaccc
     1501 acgcgcgttg aaaggcggaa aacccctcct gcagggataa cccctccacc acgagcaagt
     1561 tccacttgat tacgtgctcg ggccccgcgc ccccggcggc gagcgccgcc cgcacgttag
     1621 cgagcacctg ctccgtctgg gcggcgaagt cgcccttgcc gactatgttg ccagaggcgt
     1681 ccaccgagtc ttggccgccc acgtagaccg ttctcgcggg cccctcgacg accaccacgt
     1741 tggtgaacgc cgggttcttg ttgagcccct ccgggtcgac gtagcgcacg gacccggtag
     1801 cggggttggc ggcgtcgggt cgctcggcca tcgcgttcct cttctttaga gtacagtgcc
     1861 gtccgttaat ggtagcagag ccgctggcgg tgaagaccgc cgggttcgtc cggcgttccg
     1921 aggcggggac aagacccgtc ctcggttcct cggcacccgc gggcgactgt cgctcttcct
     1981 cacgggttcg accagagagc tctagctcgc cgccgacgaa gcgggccagc cgttcggcct
     2041 cctcctcggc gcgcctgacc cagtcaggct gatgcgcgaa gggctcgatc tcgacggaca
     2101 gccggtctcc tctgcgctcg tgccgccaga cgccctccat gcggccgtcg accagcacta
     2161 ccggcgacag ccagccctgc gggcggtaga cgtagccctt cagggcacct ggcagcacgg
     2221 cctcgcggtc ctcgtgctcg tggtcgcggc gcgctttgtt cagtttcaca cccgagaaca
     2281 tggcggcgac ttcggctctc agtactatcg gtaaccggtg gtgtccgctg gtaggccggc
     2341 gtcctggacc tctactaggt agcgccaggc gtcgggtcgc gacccgtcga ggtccgtgaa
     2401 gccgtattcc tttgcaaggg cgccgctcga gagggaacga ccgttccagc gcgcgacctc
     2461 cgggtccgcg gcgaggtgag ctacggcccg gccaacgtag cgcggcgtct cggagatgac
     2521 gaagtgcggc tggatggcag tcgcctcgcg ccagttcgac tcctccaccc caaagttctc
     2581 aagcatcatc tctgagcgta gccagccggg ggtcaaggcg accgccgtgc atccgtgggg
     2641 cgcaagctcc tgggcctggg tccacgccat gcggatcacg gaggtcttcg ccaagtcgta
     2701 gaacagcgac actctgtagt tgttctcgtt gtactcggcg gttccatccg tcatctcgac
     2761 taccagaccg ccctcgttct tgatgaggag tggcagtgcg aagtggctgg tgatgatgtg
     2821 cgtgtctatg gcgagcctca gcatccgaag gcctcgctcc agggagtgct cccacaccgg
     2881 cacattccac tcgaagaggc gctccgcacc ccacacgtcg ttgaccagca cgtccagacg
     2941 gccctgctcg tccccgatgc gccccacgag agtccgcacc tgctccggtt cgagatggtc
     3001 gacccgaacg gcgctgccac gaccgcccgc ccgatcgacg agttcggccg tctcctcgat
     3061 cgtctcggga cggtcgtact ccgagcgccc atcgcgcgtg gagcggccag tgacgtatac
     3121 tgtggccccg gccgcgccga gctcgaccgc gataccccgc ccggccccgc gcgtacctcc
     3181 ggccaccagg gccaccttcc ctttcaactt cggcaccata cgggcctcct tccgatcacg
     3241 tcaagggcat acaaggtaca cccggatcgg attgagcact tcaacgtact tccgccgaaa
     3301 gaaccactgg gcgatccagt acgacaacgt ccgtgacgaa tcgagcgata tgcgtgccat
     3361 gtcgtgtctt cttgtcacat cggctggact tggttttgtc actttatcgg ctgcgtcgtg
     3421 gggccagagt catagagtgt ctctgacgaa atcttggatg acgcgggaga gttcttcggg
     3481 ccggtcctcg gggatgagcg tgtaactgta ccgttcaccc gctttagttc cgatctctct
     3541 ggagcatcac cagtgtgatg tcgtcctcct gctcccagtc gctcccgacg aactctctca
     3601 actcggtgag gcactcgtcg atgaggtgct cgcttccgct gctgctcttc acgatctctt
     3661 ggagccgtgg aaagtcgaac atctcgcctt ggggattgtg cgcctcaacg aggccgtcgc
     3721 tgtggagcag gatgctgtcg ccgggagcga ggtaggcttc gttctcctcg taggtcatac
     3781 ctggcatggc accgagcggc attcctctcg cccgaagctc tgtgaccccg tctgccgtcg
     3841 ccacgtaggg gaggttgagc ccgcgttcgc gtagcgcagg cgaccgctat gggggtcgag
     3901 cactgcataa aggcacgtca cgaacatgtg cgcgggatgt cgggatacag gcgattattg
     3961 gcttgctcga gtaccgcacc gggagaaacg agccccggtg cgtcgccacg gaagatgctg
     4021 tgggtcgtgg tcataacgag ggcggcgggg atacccttat cagtgacgtc gccggcgacg
     4081 agcgcgagtt ggccgtcggg cagctcaagg aaatcgtaaa aatcgccgcc gacagcacgc
     4141 gccggctggt agtaggatgc gaagcgccat ccagggaggg agggcagttc cttagggagg
//
LOCUS       DATMJA010000388         6763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_407186, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000388 DATMJA010000000
VERSION     DATMJA010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407186"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VNT12_09260"
     CDS             <1..275
                     /locus_tag="VNT12_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46347.1"
                     /translation="QAEAHTEQQIIIREVTHIQPSWAERERGEPGAFTLQLVLDYGTE
                     EYILRPTVEDAEAILRLRDRIGRAYFDLQRKVLIFGNIPVQQGGSS"
     gene            412..735
                     /locus_tag="VNT12_09265"
                     /pseudo
     CDS             412..735
                     /locus_tag="VNT12_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
     gene            844..1692
                     /locus_tag="VNT12_09270"
     CDS             844..1692
                     /locus_tag="VNT12_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HWI46348.1"
                     /translation="MARILDGKAAAEVRAEVAEGVKELRSRGRAVRLDVILVGEDPAS
                     VTYVRNKQTDCAEVGMETQAHRFPQDAAQEEIEAVVERLNEDQAVSGFFIQLPLPDHL
                     DSRSLLSRIRLHKDVDGLNPESAGRLAMGLSALMPCTPHGIVQLLQRYGVSLEGEEAV
                     VLGRSNLVGKPMTQLLLRENATVTVCHSRTRDLAEVAARGDVLVVALGRREMVGAEYV
                     KEGAVVVDVGIHRRDDGGLVGDVRFEEVESKASAVTPVPGGVGPMTRAMLLFNALQAA
                     RGEVGG"
     gene            1891..2511
                     /gene="folE"
                     /locus_tag="VNT12_09275"
     CDS             1891..2511
                     /gene="folE"
                     /locus_tag="VNT12_09275"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWI46349.1"
                     /translation="MENRSLLLEDVEELYPPEHPVDLPRIERAVREILDAIGEDPDRE
                     GLRDTPARVARAYEFLFAGLHVDPTQQLTVGFQEDHHEMVLIRDIPFYSTCDHHLLPM
                     IGKAHVGYIPDGKVVGLSKLARVVEGYSRRPQLQERLTAQVADALHQSLGAKGAIVVV
                     EADHLCMTVRGVQKPGSMTVTSAVRGLYAKDQRTRQEAMSLINGQR"
     gene            2665..3306
                     /locus_tag="VNT12_09280"
     CDS             2665..3306
                     /locus_tag="VNT12_09280"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWI46350.1"
                     /translation="MRTNLIDRRRADLHEAKPSLRKRILGRRDAMDSHTRTALSRAIV
                     WDIVETSVYRRSNMIMAYASFGSELQTDEFLRHVLDQGKILLLPRVNRQRELLEVYWV
                     QDPEQDLQVGTWRIREPRPDRCARVEPAVIDFVLVPGLTFDRRGGRLGYGGGFYDKLL
                     AESLSPCAWQVAGAFESQMVEKVLVDEHDMPMDVVVTENGHYPPGPLPRWASH"
     gene            3664..6303
                     /gene="ppdK"
                     /locus_tag="VNT12_09285"
     CDS             3664..6303
                     /gene="ppdK"
                     /locus_tag="VNT12_09285"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWI46351.1"
                     /translation="MMGRGKLVYGFFEGSGEMRDLLGGKGAGLAEMTRLGLPVPPGFT
                     ITTEACRAYTEAGRLSAGLMESVAEYLEVLEGVTGKRLGDSDNPLLVSVRSGAAVSMP
                     GMMDTVLNLGLNDAAVDGLARRTGDERFAYDSYRRFIQMFGDIVLKIPHDRFEEALEI
                     LKEERGVEEDPQLEAADLKELVNVYKEIVEAEAARPFPEDPAEQLELAIRAVFDSWDN
                     ERAIAYRREFGILDSLGTAVTVQAMVFGNMGEDSATGVAFTRDPSTGEQGIFGEFLLN
                     AQGEDVVAGIRTPRPLEEMIEAMPHAFSELLETMQRLEHEYRDMQDVEFTVERDALYM
                     LQTRSGKRTAAAAIKIARDMAEEGFIRKDEALLRIDPGQLEQLLHPRMDPVAEVEVLC
                     TGLGASPGAATGKIVLTAQEAAERGGAGEAVILVRKETDPDDVEGMMRAKGVLTALGG
                     MTSHAAVVARGMGKPAVTGCSALKIDSHAGAFSVNGRVFEAGDVITIEGTSGRVIAGE
                     VPLVEPEIDEDFETILRWADRVRALGVRANADTPEDARRAREFGAEGIGLCRTEHMFM
                     EEGRLRIVREMLLSEDERALDAALEKLEPLQKEDFEKIFKAMDGLPVTVRLLDPPLHE
                     FLPDSKDLAAKVAEAEARGENAGELVRQLRIAERLEEVNPMLGLRGVRLGVLRPEVYR
                     MQARAIAAAARTVAEEGYAPVVEIMVPLVGFPSELSMIKAYIEEEVGETPVKIGTMIE
                     LPRACTVAGEIAEDADFFSFGTNDLTQMVCGMSRDDAEEKFLADYLEGGLLRENPFQT
                     LDRKGVGVFVSTACEGGREAKPALKLGVCGEHGGDPKSIAFFHEVGLDYVSCSPFRVP
                     IARLVAAQVASSAQSEKVVSAGR"
BASE COUNT         1372 a         1851 c         2324 g         1216 t
ORIGIN      
        1 tgcaagcaga agcacacact gaacagcaga tcataattcg ggaggtgacc cacatccagc
       61 cgtcctgggc tgagagggag cgcggagagc cgggagcttt caccttgcag ctagtgctgg
      121 actacggaac agaggagtac atccttcgac cgactgtgga agacgccgag gcgatactgc
      181 ggctccggga tcgcatcggg agggcctact tcgaccttca gcgcaaggtc ctgatcttcg
      241 gcaacatacc ggtgcaacag ggcggctcct cctagaggca tataagacaa ggcgatgcgg
      301 gtgcccgagg caccgcgcga gatggcgtcg gtaaggaggt gggggttccc gcctccttcc
      361 ggattgctgg acgaaagaga ggagagcaag aggatgggcg agctgacgga gatgaaatgc
      421 accgcctgcc ggggcgacga gccgacgctg acgaaccccg agatcgaaca actgcgcccg
      481 caggtctctg acgggcgcgt cctggagcgt gagggcatca agcgtctgga gcacgccttt
      541 aggttcgacg acttcgccca ggcgctgacg ttcacaaaga gggtagatgc gcaggcggaa
      601 gaggaggggc atcacccagc ccttctgacc gagtgggctt aggtgcccgt cacatggtgg
      661 acgcacaaga tcgggggact gcactggaac ggcttcatca tggcagcgag aacggacgag
      721 atcttcggag gctgaatgct cccatgcctt gtctttttgc ggaaggatcg agtggctgct
      781 acgtagagga gactgtttcc cgccgagcgg cgtcggacgg gtggtactcc gctgcggaga
      841 gccatggcta ggatactcga cggcaaagcc gccgccgagg tgcgggcaga ggtcgcggag
      901 ggcgtaaagg agctccggtc acggggcagg gcggtgcgct tggacgtgat cctggtgggg
      961 gaggatccgg cctctgtcac ctacgtgcgc aacaaacaga ccgactgcgc cgaggtcggt
     1021 atggagacac aggctcaccg cttcccccag gacgccgccc aggaggagat cgaggcggtc
     1081 gtcgagcgac tcaacgagga ccaggccgtc tctggattct ttatacagct tccgttgccg
     1141 gaccacctgg actcgcggtc cttgctctcc cggatacgcc tgcacaagga cgtggatggt
     1201 ctgaaccccg agagtgctgg gaggctcgcg atgggactgt ctgcgctcat gccgtgcacc
     1261 ccgcacggca tcgtccagct actgcagcgc tacggggtgt cgctggaggg ggaggaggca
     1321 gtggtgctcg gccgatcaaa tctggtcggc aagccgatga cccaactcct cctgcgcgaa
     1381 aacgcgaccg tgactgtctg ccactcgcgc acgcgggacc tagccgaggt tgctgcacgt
     1441 ggggacgtcc ttgtcgtcgc cctgggccgt cgggagatgg ttggggccga gtatgtgaag
     1501 gaaggcgccg tagtggtaga cgtcggtatt caccgtaggg atgacggagg actggtggga
     1561 gacgtacgtt ttgaggaggt agagtccaaa gcttcggccg taacccccgt ccccgggggc
     1621 gtcggtccca tgacgcgggc gatgctcctg ttcaatgccc tccaagcggc gcgcggcgag
     1681 gtcggaggat agtgtcgatc cggctactac ggcgtcccac ctccatgagc aggccccggc
     1741 acgcgggcgg gttaggagga ctgcgggagc gaggtcctcg tgtggctgat gcgcatccag
     1801 agggtatgcc ccacgtgcga cgagaggcta tcaagaaggt gtgtcgcgca aggcacgatc
     1861 tatcaggaca ccagaaagga aggtaaaggg atggagaaca gatccttgct cctcgaagac
     1921 gttgaagaac tgtaccctcc cgaacacccg gtagatctgc cccgtatcga gcgggccgtc
     1981 cgagagatac tggacgctat aggggaggac cctgaccgag aaggactgag ggacacgcct
     2041 gcgcgggtag cgagggctta cgagttcctc ttcgcggggc tgcacgtaga tcctacccag
     2101 cagctgaccg tcgggtttca ggaggatcac cacgagatgg tcctcattcg ggacatacct
     2161 ttctattcga cctgtgacca ccatctcctg ccaatgatag gcaaagccca cgtcggctac
     2221 atacccgacg gtaaggttgt ggggctttcg aagctggccc gggtggtaga gggttactcc
     2281 cgccgtcctc agctccagga gcgccttacc gcccaggttg ccgacgccct ccaccagagc
     2341 ctcggggcca agggcgccat cgtcgttgta gaggccgacc acctgtgtat gacggtcagg
     2401 ggtgtacaga agccggggag catgaccgtt accagcgcgg tgcgtggcct gtacgctaaa
     2461 gaccagcgca cccgtcagga ggccatgagc ctcataaacg gccagcgtta gtgaggctca
     2521 tcgaacggag tcgccgtact aattgccgag gtggcgctgg tgctgcgtcg tctttcggca
     2581 ttttcgtgga ggcgcccttc gtaagatccc gactacggcg aagattggta cgacttagat
     2641 ttcttctggg aggtaagtag aacgatgcga acaaatttaa tagaccgccg tagagcggat
     2701 ctgcacgagg ccaagccctc attgagaaag aggatactcg gtaggcgaga cgccatggat
     2761 tcccacaccc ggacggcctt gagtcgagct atagtctggg atatcgtcga gacctcggtc
     2821 tatcggcgat ccaacatgat catggcttat gccagctttg gcagcgagct acagaccgac
     2881 gagttcttgc gccacgtcct agatcagggc aagatcttgc tgcttcccag agtcaatcgc
     2941 cagagagagc tgttggaagt ctactgggta caagacccgg aacaagatct acaggtaggg
     3001 acgtggagga tacgcgagcc taggccggat cggtgcgcgc gcgtggaacc ggccgtcatc
     3061 gacttcgtgt tggttcccgg tcttacgttt gacaggcggg gcggacgctt gggctacggt
     3121 ggaggcttct acgacaaact gttggcggaa agcttatcgc cgtgtgcgtg gcaggtcgcc
     3181 ggtgcattcg agagccagat ggtggagaag gttctcgtag acgaacacga tatgccaatg
     3241 gacgtagtgg tgactgagaa cgggcattat cctccggggc cgctgccgag gtgggcgagt
     3301 cactagaagt cgtctggccg tgttgacatt ctttgataag gcgtggccgt cggtccaggc
     3361 tgctgtccag gaacgccggc ctcggcaggt cgagaaggtg gagtgccgcc tggtgcaagc
     3421 ccgaggcgtt gttgcgaaat ttgacggcct cctgttgggg gtagcttcgc atcttcaagt
     3481 ctcctaaaga aagaggtttc aaagctgatc acccttgact cagtgcagaa ctttatggta
     3541 tctgttccat tatgagcaac attaacaaat ggcgcaacaa aattgaggtg atgtggtggg
     3601 gcgtcgcaga cactcggtaa ccggacgggc tgcgtgcgaa gagcgcgtaa aggggcgcat
     3661 ccaatgatgg gtaggggcaa attggtctat ggcttcttcg aggggtcggg ggagatgagg
     3721 gatctactcg gtggcaaggg agcaggactg gctgagatga cccgcctcgg cctgccggtt
     3781 cctccggggt ttacgatcac cacggaggct tgccgagcgt acacggaagc tgggaggctc
     3841 tctgcgggtc tcatggagtc cgtggccgag tacctagagg ttcttgaggg ggtaaccggc
     3901 aagcgtttag gggattctga taatcctctg cttgtctcgg tacgtagcgg agcggcggtc
     3961 tccatgccgg gcatgatgga cacggtattg aacctcggcc tcaacgatgc tgccgtggac
     4021 gggctcgcgc gccgaaccgg ggacgagcgc ttcgcttacg actcctaccg gcgcttcatc
     4081 cagatgttcg gggacatcgt gctcaaaatc ccgcatgaca ggttcgagga ggccctcgag
     4141 atcctcaaag aagagcgcgg ggtcgaggag gacccgcagt tggaagccgc ggatctcaag
     4201 gagttggtga acgtttacaa ggagatcgtc gaggcggagg ccgcccgtcc gtttccagag
     4261 gatcctgcag agcagctcga actcgcgatt cgggcagtct tcgactcctg ggacaacgaa
     4321 agggccatag cctaccgcag ggaattcggc attctggact ccctgggcac cgccgtcacg
     4381 gtgcaagcca tggtcttcgg caacatgggc gaggactcag ccactggggt agcattcacg
     4441 cgtgacccgt caaccgggga gcaggggatc ttcggggagt ttttgctcaa cgcccaaggc
     4501 gaagacgtcg tggcgggtat ccgtacgccc cggcccctgg aggagatgat agaggcgatg
     4561 ccccacgcct tctccgaact tctcgagacg atgcagagac ttgagcacga ataccgtgac
     4621 atgcaggatg tggagttcac cgtggagcgg gatgcactct acatgctcca aacgaggagc
     4681 ggcaagagga ctgctgcggc ggcgatcaag atcgcccggg acatggctga agaggggttc
     4741 atccgcaagg atgaggctct actgcgcata gatcccggcc agctggagca gctgctccac
     4801 ccgcggatgg accccgtagc cgaagtcgag gtcctgtgca cgggccttgg agcgtccccc
     4861 ggagcggcca cgggcaagat tgtcctgacg gcccaggagg ccgccgagcg cggcggggcc
     4921 ggggaagcgg tgatcctggt acgtaaagag accgaccccg atgacgtgga gggcatgatg
     4981 cgcgctaaag gcgtgctgac ggccctgggc ggcatgacca gccatgcggc ggtggtagcg
     5041 cggggtatgg gcaaaccggc ggtcaccggg tgttcggctc tgaagataga ctcccacgcc
     5101 ggcgcgttca gcgtgaacgg cagggttttc gaggccgggg acgtcatcac catcgagggt
     5161 acgagcggca gggtgatcgc gggtgaagtc ccgcttgtgg agcccgagat cgacgaggac
     5221 ttcgagacaa ttctccgctg ggcagacaga gtacgcgcac tcggggtccg ggcgaacgcc
     5281 gacactccgg aggacgcccg tcgcgcccgc gagttcggcg ccgaagggat cggactgtgc
     5341 cgcaccgagc acatgttcat ggaggagggg cgcctccgga tagtgcggga gatgcttctc
     5401 tccgaggatg agagagcctt ggacgcggca ctggagaagc tagaacccct ccaaaaggaa
     5461 gacttcgaga agatattcaa ggccatggac gggctgccgg tgaccgtacg cctcctggac
     5521 ccgccgctgc acgagttcct ccccgactcc aaggacctag cggcgaaggt agctgaggcc
     5581 gaggcccgcg gagagaatgc cggcgagctc gtgagacaac tgcggattgc cgaacgactg
     5641 gaagaggtca acccgatgct tgggctgcgg ggggtccggc ttggagtact gcggccggag
     5701 gtctaccgga tgcaggcgcg ggccatcgct gccgccgcta gaacggtagc cgaggaagga
     5761 tacgccccgg tggtagagat catggtgccg ctggtcggtt tcccctccga gctgtcgatg
     5821 ataaaggcct acattgagga ggaagtgggg gagacgcccg taaagattgg gaccatgatc
     5881 gagctgccca gggcatgcac cgtcgccggc gagatagccg aagacgcgga cttcttttct
     5941 ttcggcacca acgatctcac ccagatggtg tgcggaatga gccgcgacga cgccgaggag
     6001 aaatttctcg ccgactacct ggaaggtggt ctgctgcgag agaatccatt ccaaaccctc
     6061 gaccggaagg gcgtcggagt ctttgtctcg accgcctgcg aaggtggtag ggaggctaag
     6121 ccggcgctca agctgggagt ctgcggcgag cacggcggcg atccgaagag catagccttc
     6181 ttccacgagg ttggcctcga ctacgtctcc tgctcaccct tccgggtgcc gatcgcccgc
     6241 ttggtcgcgg cccaggtcgc ctcgtcggca caaagtgaga aagtggtgag cgcaggtagg
     6301 tagtcttgag cgagatcggc gggacttcga cgccctttcg ttcggaggag agatagaatg
     6361 gaggtagtcc caaacgtgaa gacgtggatc gggccgcggc acgacgcccc cgatttaaag
     6421 ctgaaagctc ccaggctgtg gtcgagcaga cgaggagaac gaaaaacgta gcggctcatc
     6481 tgatggtcgt tggagcccgc acggtggtct ttaccgagaa caccgtgcgc cgctcaaaaa
     6541 gcgggcgagg gtgcttgact cggcggcggg cgtatgttag ttgtttcact tatggaaaag
     6601 cggtgcgtac tgcgcaacaa caatcggtct gctggcgtat gaggtccgtg ggggatcttc
     6661 gccggaggtg gagcgagcct cttgttcgtc ctgagttgct cgggcgttgc gtcctcgtct
     6721 ccgcgagacg tacggaggaa tagcggtgga agatagggct agg
//
LOCUS       DATMJA010000389         4269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_407390, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000389 DATMJA010000000
VERSION     DATMJA010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407390"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /locus_tag="VNT12_09290"
     CDS             <1..1890
                     /locus_tag="VNT12_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWI46352.1"
                     /translation="GIGSTLIARSEFEEAETALVEAADLFATADNAPLLSGVMLEQGA
                     LQEARGDHGTAVATVRRALHLVSGKDWSVQRVYAHLRLADLLLPDAVEAESHLLEASK
                     LAERLALPHLRYRLNERLGSLRRLQGRDEEARVLLEAAVDEIERLRGTVAHETTRASF
                     LRDKITAYEELLKLHLARGSEEDSHSAFAIAERAKSRALVDLLTGVVQGSATSADDAV
                     EGRIRDLQSDLNVTYSRMFGGTDDDRHGMPLPDLRGRAVELEREISQLRLRAAATSDI
                     FAPPAVSDGLQHLPSDVTLLAYHLVGDEIVTFFQGQGGIRVIRNPGSAATVARLVQQL
                     DVQWDRSGTGREFAERHMALLERLTQRVLTSLYRELIGPLEPLFKETADPTSDRTVAS
                     RKLVIVPHGLLHRVPFHALFDGGSYLLERFEVSYAPSAKVYSLCQKRSSRGFDKALVV
                     GVADPSIPAVAEETQAVVRHLPAAELLRDQQATVEALRLKVPGCGVLHLACHGMFRVD
                     NPMFSSLKVGDGWLAASDVIRLDLAGALVTLSACESGRNEVYAGDELMGLTRAFLGAG
                     ATTLVASLWLVQDETTAELMENWYEHLSEGVGRATALRNAQLALKDKYPHPYYWAPFV
                     LIGQR"
     gene            2184..2780
                     /locus_tag="VNT12_09295"
     CDS             2184..2780
                     /locus_tag="VNT12_09295"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWI46353.1"
                     /translation="MEGVYPLIEHYGYLIVFFGVMLGTTGIPFPSAAILLAAGVLVQQ
                     GHLDLRDVILFGILGAIIGNQIGYWIGHRAGRPFVLKWGRYVKLTPERLERVEGLFAR
                     HGGKAVFAARFFSVSRLLEALVAGMSRMHWGTFLFYSALGGAVWATAVVLLGYFFGES
                     WGSAHGWSGRAPLLLVLLLGVALGFYLTYRWTTSRRSG"
     gene            3198..>4269
                     /locus_tag="VNT12_09300"
     CDS             3198..>4269
                     /locus_tag="VNT12_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWI46354.1"
                     /translation="MAAVKEPGPEDRTVSPERRLELLAGVPAFSHLPAPVLEDLASRL
                     TEERFRSADRVVVEGDTDDRLYMIVEGRAEASTIGPSGIVPLAALGPGELFGELSLLE
                     SGSRRKATVTAVESLLLLSLRATDFRLALDAHPEGRSAFEKLADDLLVTKLLKQASPF
                     STLDGERLRRLAARLERLEVPTGETIIRQGEAGEECYVLRSGRVEVLAMGAQGDERIL
                     ATLDPGSLFGEAALLTDGPRNATVRATEPCTLLVLRRTDLLEVLGEDRQTRERMLELV
                     RMRDRPRRVRGIEVHHRTTITGETITTLKDARRDTNHRLSPAGWFVWQRLDGEHTLRD
                     LTSEYLATHEASASHAVVEAVVG"
BASE COUNT          767 a         1282 c         1386 g          834 t
ORIGIN      
        1 ggcatcggct caacgttgat cgctcgctcg gagttcgaag aagccgagac cgcactggtc
       61 gaagccgcag atctttttgc gacggccgac aatgcgccgt tgctctcggg ggtgatgctc
      121 gaacagggtg ctcttcagga ggcacgcggg gatcacggaa cagccgtcgc aaccgtgagg
      181 cgggcactgc acctcgtctc cggaaaagac tggtcagtgc aacgcgtcta cgcgcatctg
      241 cgtctcgcgg acttgctgct cccggacgct gtcgaagcag agtcgcacct tctcgaggca
      301 agcaagctgg ccgaacgtct ggctctgcca cacctgcgct accgactgaa cgagcgactc
      361 ggcagcttga gacgactgca aggtcgcgat gaggaggcgc gggtgctgct cgaggcggcg
      421 gttgacgaga tcgagcgcct gcggggcacc gtcgcccacg agacgacgcg cgcttccttc
      481 ctgcgggata agatcaccgc ctacgaggag ctattgaagc tacatcttgc aagggggagc
      541 gaagaggact cgcatagcgc cttcgccatc gccgaacgtg ccaagtcgcg tgccttggtg
      601 gacttgctca ccggggtcgt acagggatcg gcaacatcgg cagatgatgc ggtggaaggg
      661 cggatccgcg atttacagtc ggacctaaac gtgacttata gtcgaatgtt tggcggcacc
      721 gacgacgata ggcacgggat gccgctccct gacctgcggg ggcgcgcggt cgagctcgaa
      781 agggagatca gccagctgcg cttgcgcgct gccgcaacgt cagacatatt cgcgcctcca
      841 gcggtgtcgg atggcttgca gcatcttccg tccgacgtga cgttgctggc ctaccaccta
      901 gtaggcgatg agatcgtgac gttctttcag gggcaaggcg gcatccgggt gatccggaat
      961 cctggctcag cggcgacggt cgcgcgtctc gtgcagcaac tcgacgtgca gtgggatcgg
     1021 tcgggtacgg ggagggagtt tgcagagcgg cacatggcgt tgctcgagcg gttgacgcaa
     1081 cgggtgctga cttcgctata cagggagcta atagggccgc tggagccgct cttcaaggaa
     1141 accgcggacc cgacatctga cagaaccgta gcgtcgcgca aactcgtaat cgtgcctcac
     1201 ggtctgctgc atcgggtgcc gttccacgct ctattcgatg gcggatcgta tcttttagag
     1261 cggttcgaag tctcctacgc cccaagtgcg aaggtttact cgctctgcca gaagcgatcc
     1321 tcacgaggat tcgacaaggc ccttgtggtg ggcgtggccg acccttcgat accggccgtg
     1381 gcagaggaga cgcaagccgt cgtccggcac ctacctgctg ccgagctgct gagagaccag
     1441 caggccactg tggaggcgct gcgtttgaag gtgccaggtt gcggcgtctt gcatctggcc
     1501 tgtcacggca tgtttcgggt ggacaacccc atgttctcat cgttgaaagt aggcgatggt
     1561 tggctggcgg ccagcgacgt cataaggctt gatcttgcag gcgccctcgt caccttgagt
     1621 gcctgcgagt cggggcgcaa cgaggtctac gctggcgatg aactcatggg cctgacacgg
     1681 gcgttcctcg gggctggggc gacgacgctc gtggccagct tgtggctcgt gcaggacgag
     1741 acgacggcgg agctaatgga gaattggtac gaacacctga gcgaaggcgt cggacgggcg
     1801 acagccttgc gcaacgccca gctcgcgctc aaagataaat acccacaccc atattattgg
     1861 gcaccatttg tcctgatagg ccagcgataa cgctaccacc cactagacaa gaagaggtat
     1921 gcgaggtcgt tttcgatggc acgtgccagt tcgacctcgt cacctagggt ctcgagctca
     1981 gcccgatccc tactctccca ctggtatttc agcccgtatc cggtcagcag gcaaaccacc
     2041 acatcgcgga tctaccgatg tacccagatc gtttggcggc ggtagccccc gtgagtgagg
     2101 ccgcggcctc aggctgtagg atttctctgg gccagttccg agagcctctc aggagcggct
     2161 gtgatccgta tacttcgttt gccatggaag gtgtctatcc cctgatcgag cactacgggt
     2221 acctgatcgt cttcttcggc gtgatgctcg ggaccacggg catccctttc ccgagcgcgg
     2281 ccattctcct ggcggccggt gtactggttc agcagggtca cctggacctc agggacgtca
     2341 tactgtttgg catccttggg gctatcatcg ggaaccagat cggttactgg atcggccaca
     2401 gagcgggacg accgttcgtc ttgaagtggg gacggtatgt aaagctcacc ccagagcgcc
     2461 tggagcgagt cgaagggctc ttcgcacgcc acggcggcaa ggcggtcttc gctgcccgtt
     2521 tcttctcggt ctcccgcctg ctcgaagcgc tggtagccgg catgagccgc atgcactggg
     2581 gcacgttcct cttctacagc gccctcggtg gggcggtgtg ggctacggcg gtcgtcttgt
     2641 tggggtactt cttcggggaa agttggggct cggcgcacgg ttggtcagga cgcgctcctc
     2701 tcttgctagt tcttctactc ggggtggcgc ttggcttcta cctcacctac cggtggacaa
     2761 cctcccgcag gagtgggtag gcttctctca gatgtacttg ggcccatcct ttcagagcat
     2821 gatgatagct ctgggtaagc aacgcgtccg cggctcagca ccctcaccac gggctgtaga
     2881 ggtattaccc gggcgtccgc tacgacctgg tttgccggaa ggattgttca attctcgctt
     2941 gtgacctgca cgacgcagaa gcggttgccg tcgggatctt ccaacacgat ggcgcccttc
     3001 gcactcgccc cgctaccgag gcgtttctgt catggagact tgagacgacc aacgcgatgg
     3061 tccgtcccgg ccctccaacg taccttgctc tgatcgactt gggagcgtgc ccggcacgag
     3121 ccgcttcatc ttgatccgag cgcgggctgg tgttaaatga tcactggcga gaaacatcgg
     3181 aggctaggaa agggtttgtg gccgccgtga aggaaccggg accggaggat cggaccgtct
     3241 caccggagag gcgcctggag ctactggcag gcgtaccggc tttctcgcac cttcccgctc
     3301 cggtgctgga ggatctggct tcacgcttga cggaggagcg cttccggtct gccgataggg
     3361 tagtggtaga gggtgacacg gacgaccgac tctatatgat cgtggaaggc cgggccgagg
     3421 catcgacgat cgggccctca ggcattgttc ctctggcggc gctagggcct ggggagctgt
     3481 tcggcgaact atctctgctc gagtcaggaa gcaggcggaa ggccacggtt accgccgtcg
     3541 agtccctcct cttgctaagc ctgcgcgcaa cagacttccg cctggcactc gacgcccatc
     3601 ctgaaggccg atctgccttc gaaaagctgg cagacgacct gttggtaacg aagttgctca
     3661 aacaggcaag tcccttctcc acgctggacg gcgagcggct gcggcgcctc gcagcccggc
     3721 tcgagcgtct ggaggtcccc accggagaga ccatcatccg ccagggcgaa gccggcgagg
     3781 agtgctacgt gctccgaagc ggtcgtgtgg aggtgctcgc gatgggagcc cagggcgacg
     3841 aacgcattct ggcaacgtta gatcccggct ccctcttcgg cgaggcggcc ctgctcaccg
     3901 acggaccacg caacgccacg gtgagagcca ccgagccgtg cacactgctc gtgctgcgtc
     3961 ggaccgatct acttgaggtc cttggcgagg atcggcaaac gcgcgagcgt atgctcgagc
     4021 tggtgcggat gcgggatcgc cctcgccggg tgcgtgggat agaggtgcac catcgcacca
     4081 ctattaccgg agagactatc acgaccctca aagacgccag gcgcgatact aaccatcgcc
     4141 tctcccccgc cggctggttc gtgtggcagc gtctcgacgg ggagcacacc ttgcgggatc
     4201 tcacgtcgga gtacctggcc acgcacgagg cgtccgcgtc gcatgccgtg gtagaggcgg
     4261 tagtcggac
//
LOCUS       DATMJA010000390         2950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_170881, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000390 DATMJA010000000
VERSION     DATMJA010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170881"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VNT12_09305"
     CDS             <1..548
                     /locus_tag="VNT12_09305"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWI46355.1"
                     /translation="LMTPPRIRFEAEDTLRFGEFGVTAKLTGGDGPREKGAPQDTSGQ
                     TRIFRTEESSGGEIDQATATISPDEARRHGLAREIVELVLGDEKHHLEGRGPWSVGRS
                     QENDIVISDPNVSRKHARISRADNGFVVEDLGSTNGTLLDGAPIDRERIEGGDELTFG
                     QSTARFIRRIDSPGEESGGRR"
     gene            552..1037
                     /locus_tag="VNT12_09310"
     CDS             552..1037
                     /locus_tag="VNT12_09310"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWI46356.1"
                     /translation="MLELQVPLLAAKALILLLLFAFIYAVMSRSIGDLRKIPDDEPYV
                     PGRGHNGRGTNARRGSVSGMPELVVEDSEILAPETRFELGDGDTSMGRSSGSTIVLRS
                     DDYASGRHAQLTRHGGLLYVEDLDSTNGTFVNGRKTVGATPLRNGDTVRVGSTTFRYE
                     E"
     gene            1042..2235
                     /locus_tag="VNT12_09315"
     CDS             1042..2235
                     /locus_tag="VNT12_09315"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWI46357.1"
                     /translation="MPFLELQPFGLTHAGKVRQNNEDALLVGEGEDETLFVVADGIGG
                     FEAGEVASSLAVDVLKDLQPDEPFKAAIGEANRRIVAAGRVDEKLSGMGTTVVAIRFG
                     GKQGEPVVEVAHVGDSRAYLMRGGVMNPITEDHSLVAELVRSGDLTRDQAAEHPQKNL
                     ITRALGADEEVDVDTAVLSVEAGDRILLCSDGLSDMVSEAGISEILADSPDDPERAAR
                     GLLSAALDAGGNDNITVVVVDVKERPAPREADARDERERRSSGISELLKGTDARTPSR
                     GKAAPSRPRRPTRAARRKRPSRLRKALGKLVRGLALVLVLVAALTPVYLWGSSRYYFE
                     FDKGEVVAYQGLPYAPLGIELNQEWRRPGLTESEIKEPYQKPIENHKLYTRDQAERVL
                     EGLGS"
     gene            2237..>2950
                     /locus_tag="VNT12_09320"
     CDS             2237..>2950
                     /locus_tag="VNT12_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWI46358.1"
                     /translation="MTQRAFTPMILALLVTTLGFATLILVQEAASPPLVYGAYYAGTL
                     FALYLGVRLLLPHSDVLLLPIVTLLTGLGLVMIFRLTYNVEGAQELALTQAVWILMGS
                     GAMFLLVLFFRNYDRIFDYKYLLAIAAVFLIASTFTPLGYTVNGARLWVDLGPVNFQP
                     SEFARIALVVFFAGYLAEKRDVMAATSRSFLGIQLPAIKYFGPVALVWAASFGLLVFE
                     KDLGSSLLFFAVPLLMLYAA"
BASE COUNT          536 a          960 c          956 g          498 t
ORIGIN      
        1 atcttatgac tcccccaagg atacgcttcg aggccgaaga cacactccgc ttcggtgagt
       61 tcggcgtcac cgccaagctg acaggcggcg acggtccccg cgagaagggc gccccgcagg
      121 acacctcggg ccagactcgt atcttccgca ccgaggagtc gagtggcggg gagatagacc
      181 aggccacagc cacgatatcc cccgatgagg ccaggcgcca cggtctggcg cgggagatcg
      241 tcgagctcgt tcttggggat gagaagcacc acttggaagg gcgcggcccg tggagcgtgg
      301 gccgctcgca ggagaacgac atcgtaataa gcgacccgaa cgtgtcgaga aaacacgcca
      361 ggatctcacg cgccgacaac ggctttgtcg tggaggacct cggttctacg aacggcaccc
      421 tgctagacgg cgcccccata gatcgcgaaa ggatcgaagg cggcgacgag ttgaccttcg
      481 gccagagtac ggcccgcttc atccgccgca tagactcacc gggcgaggag tcgggcggga
      541 gacgttagag gatgctcgag ctccaggtgc cgctgctcgc ggcgaaggct ctgatcttgc
      601 tcctgctctt cgcctttatc tacgccgtaa tgagcaggag catcggggac ctgcggaaga
      661 taccggatga tgagccctac gtgccgggcc ggggccacaa cggacgcggg accaacgccc
      721 gccggggctc agtctctggg atgcctgagc tggtggtcga ggactcggag atcttagccc
      781 ccgagacgcg gttcgagctc ggggacgggg ataccagcat ggggcgctcg tcaggcagca
      841 ccatcgtcct gaggagcgac gactacgcct cgggacgcca cgcccagctc accaggcacg
      901 ggggtttgct ctacgtcgag gacctcgact cgacgaacgg gactttcgtg aacggacgca
      961 agaccgtggg cgccacgccc ttgaggaacg gggacaccgt cagggtcggc tcgaccacct
     1021 tcaggtacga ggagtaggac cgtgcctttc ctcgagttac agccattcgg cctcacccac
     1081 gccggcaagg ttcgccagaa taacgaggac gccctgctcg tcggcgaagg tgaggacgag
     1141 acgctcttcg tcgtcgccga cggcattggc ggcttcgagg ccggggaagt cgcgagctcc
     1201 ctcgccgtag acgtgctcaa agatctccag ccggatgaac ctttcaaagc ggccatagga
     1261 gaggcaaacc gaaggatagt ggccgccggc cgcgtcgacg agaagctctc ggggatgggc
     1321 accaccgtgg tcgccataag gttcggtggg aagcaggggg agcccgtggt cgaggtggcg
     1381 cacgtcggtg attcgagggc ctacctcatg cgcggcggcg tcatgaaccc cataacggaa
     1441 gaccactcgc tcgtcgctga gctcgtgcgt agcggcgacc tgaccaggga tcaggcagcg
     1501 gagcaccccc agaagaacct catcacccgc gccctcgggg cggacgagga ggtcgatgtc
     1561 gacaccgccg tactctccgt agaggccggc gaccgcatcc tgctctgttc tgacgggctc
     1621 tcggacatgg tctccgaggc agggatctcg gagattctgg ctgattcccc ggacgacccg
     1681 gagagggcgg cgcgcggcct gctctccgcg gccctcgacg ctggcggcaa cgacaacatc
     1741 accgtcgtcg tcgttgacgt aaaggagcgg cccgcgccgc gcgaggcgga cgcgcgagac
     1801 gagcgtgaga gacgctccag cggcataagc gagctgctaa agggcacgga cgcccgcacg
     1861 ccatccaggg gcaaggccgc cccgagccgc ccccggcggc ctaccagggc cgcgcgccgg
     1921 aagcggccta gtcgcctcag gaaggcgctc ggaaagctcg tgcgggggtt ggcgcttgtc
     1981 ctggttctag tcgccgccct gacgccggtc tacctctggg ggtcaagcag gtactacttc
     2041 gagttcgata agggtgaggt ggtggcctac cagggtctgc cctacgctcc tttaggcatc
     2101 gagctcaatc aggagtggcg caggccgggc ctcacggagt cggagatcaa agagccctac
     2161 cagaagccca tagagaacca caagctctac accagggacc aggcagagcg ggtcctcgag
     2221 ggcctgggta gttagggtga cccaacgcgc cttcacgccc atgatcctgg cactgctcgt
     2281 gaccacattg ggtttcgcga ccctcatcct cgtccaggaa gcggcgagcc cgcccctcgt
     2341 ctatggcgcg tactacgcgg gaactctctt cgcgctctac ctcggggtga ggctcctttt
     2401 accccactcc gacgtgcttc tgctgccgat agtcacgctc ttgacgggac tagggctcgt
     2461 catgatcttc cgcctgacat acaacgtgga gggggcacag gagctcgccc tgacacaggc
     2521 ggtctggatc cttatgggga gcggcgccat gttcttactg gtgctgttct tccgcaacta
     2581 cgaccgcatc ttcgactaca aatacctgct cgccatcgcg gcggtcttcc tgatcgcctc
     2641 cacgttcacg cccctcggtt acaccgtaaa cggggcgcgg ctctgggttg acctcgggcc
     2701 tgtcaacttc cagccctcgg agttcgcccg catcgcactc gtggtcttct tcgccgggta
     2761 cctggccgag aagcgagacg tcatggcggc gacgagccgc agcttcctcg gtatccagct
     2821 ccccgcgatt aagtacttcg gacctgtagc cctcgtctgg gccgcctcct tcggcctcct
     2881 agtcttcgag aaggacctcg gctcgagcct cctgtttttc gccgttcccc tgctgatgct
     2941 ctacgccgcg
//
LOCUS       DATMJA010000391         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_466697, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000391 DATMJA010000000
VERSION     DATMJA010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466697"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..881)
                     /locus_tag="VNT12_09325"
     CDS             complement(261..881)
                     /locus_tag="VNT12_09325"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWI46359.1"
                     /translation="MTRPTGLPPEECSLYAFDAAWVLGRGGPLAGADEAGRGALAGPL
                     VAAAVVLGDAEVSGINDSKLLRPSVREEIFGALLRRAEAISVVAYPAWWIDRHGVGLA
                     NREALSRALDSLRPCAGCGLADGNLALGPEVECLPRADARSAAVAAASVVAKVLRDDA
                     MRALSETHNGYGFGRNRGYGTPEHRLALSELGPCRVHRLSYAGVGD"
     gene            complement(901..1227)
                     /gene="rplS"
                     /locus_tag="VNT12_09330"
     CDS             complement(901..1227)
                     /gene="rplS"
                     /locus_tag="VNT12_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWI46360.1"
                     /translation="MITSDNLQQRDVTFKPGDTVRVHYRVIEGNRERVQIFEGLCISR
                     KGSGIHETFTVRKNSFGVNVERIFPLHSPKIAKIEVASRGAVRRAKLYYIRDKVGKKA
                     RVKKAR"
     gene            complement(1211..1954)
                     /gene="trmD"
                     /locus_tag="VNT12_09335"
     CDS             complement(1211..1954)
                     /gene="trmD"
                     /locus_tag="VNT12_09335"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWI46361.1"
                     /translation="MRSIDIFCVFPQAVDAAVGVGVVGRAIENGIVGYRSFDLREYSP
                     DGRIDDMSYGGGAGMVVRVDVVARALEEVYGVPAREVRTGRRVVLTEPAGRRVTQGYV
                     DEVASGPDLTVICGRYGGIDERVWSGLATEALSLGDFVLSGGEIVAAALADAVIRRLH
                     GVLGNAESLIGESFSAEDMVGPPTYTRPAIWDGEEVPGVLLSGDHAKIRAWREREALE
                     RTRARTTGRRSPWPSGDPPTRGEEHDNER"
BASE COUNT          356 a          719 c          637 g          407 t
ORIGIN      
        1 tggatttctg gtgtagatgt agtgttcggc tacggagcgg agggtgtttt gcttgcggtg
       61 ggtgacggct tccagtgggt ctccgtagcc ggtaccgcgc cgcagcttga cctccacgaa
      121 gacgagggtg tcgtccttgc gcactatcag gtccatctcc ccgcgccggg ttctgtagtt
      181 gcgctcgaga agtttgtaac cgtgttggat cagatagcgc agggcgaggt cttcacccca
      241 tgctccgggg gatttattct tcagtctccg accccggcgt agcttagacg atggacccgg
      301 cagggaccga gttcggagag ggcgagccgg tgctcgggcg tcccgtaccc tcggttacgt
      361 ccgaacccgt agccgttgtg ggtctcggaa agggcgcgca tcgcgtcgtc gcggaggacc
      421 ttggccacaa cgctcgcagc tgcaaccgca gcgctcctgg cgtcggccct gggcaggcac
      481 tccacctcag ggccgagcgc caggttgccg tcagcaagac cgcacccggc gcaaggccta
      541 agtgagtcga gagccctgct cagggcttcc cggtttgcca ggccgacgcc gtgacggtcg
      601 atccaccagg ccgggtaggc tactacggat atggcctcgg ccctgcggag cagcgcgccg
      661 aagatctcct cccgcacaga gggccgcagc agtttggagt cgttgatccc ggagacttcg
      721 gcgtcgccaa gaactacggc agcggccacc agcggtcctg ccagcgcgcc acgtccagcc
      781 tcatcagccc cggccagcgg cccgccgcga cccagcaccc atgccgcgtc gaaagcgtag
      841 agggaacact cctccggcgg aaggccggta gggcgagtca ctgacaacgc cggtgcctgc
      901 ttacctggcc ttcttgaccc tggctttctt gcccacctta tcgcggatat agtagagctt
      961 cgcccgtctc acagcgccgc gcgaggcgac ttcgatcttg gcgatcttcg gggagtggag
     1021 cgggaaaatc ctctccacgt tcaccccgaa ggagttcttt ctgaccgtga acgtctcgtg
     1081 aattccgctg cccttgcgcg agatacacag accctcgaag atctgcacac gctcccggtt
     1141 gccctcgata acgcggtagt gaacccgtac cgtatccccc ggcttgaacg tgacgtcccg
     1201 ctgctgcaag ttatcgctcg ttatcatgtt cttctcctct cgtgggtgga tctccggatg
     1261 gccagggcga ccttcttccg gtcgtccttg cccgtgtacg ttccagcgcc tcgcgttcgc
     1321 gccaggcgcg gatctttgcg tgatcgcccg ataatagcac gcctggcacc tcctctccat
     1381 cccagatggc aggccgcgtg taggttggag gaccgaccat atcttcggcc gagaacgact
     1441 cgcctatgag gctctcggcg ttaccgagga cgccatgtag acgcctgatc acggcatcag
     1501 cgagcgcggc ggccacgatc tccccccccg acaatacgaa gtcgcccagc gagagggcct
     1561 ccgtggcaag cccgctccaa acccgctcgt ctatccctcc gtagcgtccg cagatgacag
     1621 tcaggtccgg ccccgaggcc acctcatcga cgtagccctg tgtgaccctg cgtccggctg
     1681 gctccgtgag tacgacccgc cgccccgttc ttacctccct cgccggaacg ccgtagacct
     1741 cctcgagcgc ccttgccacg acgtcgaccc tgacgaccat cccggcaccg ccaccgtatg
     1801 acatgtcgtc gatgcggcca tccggagagt actcacgcag gtcaaagctg cggtagccga
     1861 cgatgccgtt ctctatggcc ctgccgacga ccccgacgcc gacggcggcg tcgaccgctt
     1921 gggggaaaac gcagaagatg tctatgcttc tcaccttcta gccttccggc ggtcggatca
     1981 gaacctgccc ggcccagagg tctacctcgg ggacgtgctc gagggtaaag gggagataga
     2041 ggtcttgtcg atcctcttct tcacggacca cgaggatttc gtgggcggca gtctcgaacg
     2101 tatcaatcac ctttccgac
//
LOCUS       DATMJA010000392         7248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_526032, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000392 DATMJA010000000
VERSION     DATMJA010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526032"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VNT12_09340"
     CDS             <1..229
                     /locus_tag="VNT12_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46362.1"
                     /translation="RKGIEDLSHWYPGADFRPLALFRILYGQFAQPPGIHSSLSENMT
                     QTPGLAGEYTEDSSISGSMLSGEKAAGAVIA"
     gene            226..978
                     /locus_tag="VNT12_09345"
     CDS             226..978
                     /locus_tag="VNT12_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI46363.1"
                     /translation="MSANGTPALKIEKLTKTYSNGFLALDGVSLEVEAGQFFGLLGPN
                     GAGKTTLINGVVSLARPDSGSVEVFGKDAYREFREARRMIGVSPQEVNLDKFLTVEET
                     LLFHAGYYGVPRKKARERAEELLERFELTGKRGQRTNTLSGGMKRRVLFARALMHDPK
                     VLFLDEPTAGVDVELRYKLWGYIRELNGGGMTILLTTHYLEEAEALCEEIALINGGNI
                     AAQGSSEDLKERYEARNVEEVYMKVIGHDLHG"
     gene            962..1729
                     /locus_tag="VNT12_09350"
     CDS             962..1729
                     /locus_tag="VNT12_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI46364.1"
                     /translation="MIFTDRTFQTLLSREILRFTRVWMQTIVPQLLTSLLYLVVFGIA
                     LGGRIKQIEGIPYLDYILPGIALMSLITNSQMNSSWSVFDAKRERYIDEVLISPMSDL
                     QITLAYSLGGMLRGLVMGAGVFIVGVPFVGVQIKHPLMLVVIGLLASFAFSSFGTMVG
                     ALATRIEHISLITSVIIQPLAFLGGVFYSVGMLPGVLKVVTFFNPIFYTVDAARYVTL
                     GVSDLNPYPTLGMVCLIATICFGGAWWAISRGPNLRY"
     gene            complement(1853..2152)
                     /locus_tag="VNT12_09355"
     CDS             complement(1853..2152)
                     /locus_tag="VNT12_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46365.1"
                     /translation="MIVLTIGTALIHLYLGLQGFPLFVLNGLGYLTLLAALILPIPRI
                     SDYRNLTRWILLGYTALTIFLWIVVGARTVIGYTDKIIEIVLIALLVLEASRSRR"
     gene            complement(2172..3443)
                     /locus_tag="VNT12_09360"
     CDS             complement(2172..3443)
                     /locus_tag="VNT12_09360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI46366.1"
                     /translation="MTSDVWRRRSPLVFLFVTVFVDMIGYGIVVPLLPFYAGPYASGA
                     VLVGLLGSLYAAMQFVGGPFLGGLSDRHGRRPVLLLCLLGTSIAYLLLGLAQTLASLV
                     LAVVVAGAASGTLATAQAYIADSTAKEDRARGLGLIGAAFGLGLMAGPALGGLLSLHS
                     LSAPALFASALALTNCVFGYLTLPESLASHLRRKVPLLRLDPISQLAGILKMRHIRAL
                     LAAVLLLNLALAGLVNNFPLFSQVRFGWGTTANAFFFAFVGVCAVITQGFLIGRLQPR
                     FGESRLLVGGLVLVSLNLLLVALVPSGVLLYPVVGLLALGMGLAIPSLTAIISNRTPA
                     ETQGRLMGGLQAILSLAMILGPATAGLAFDHLGIPAPYLIGGALIILALIVSAADLLP
                     GRRTRVVQRAKISGQAQAGKEEEHSLYERLH"
     gene            complement(3440..4189)
                     /locus_tag="VNT12_09365"
     CDS             complement(3440..4189)
                     /locus_tag="VNT12_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWI46367.1"
                     /translation="MESIARIPARRLAHQILTYDTIVGETGLGTGGAWALKRFSRNTS
                     IEGQENVPRGGPLLLVSNHPGLADAVALFATIPRDDLRVIAADRPFLEVLPNTSRHLL
                     TVTETFAGRSGVVRAAARHLRGGGAVLTFPGGRIEPDPAVLPGAVEALNHWSSSADLL
                     ARLTPGLVVVPVVVSGVISPSALRNPLIHLRRRRRDREWLAATLQVLVPALRNVDAMV
                     EFGSPIHAEPGAVGDAVIEQTRRLIENRETA"
     gene            complement(4506..4907)
                     /gene="folB"
                     /locus_tag="VNT12_09370"
     CDS             complement(4506..4907)
                     /gene="folB"
                     /locus_tag="VNT12_09370"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWI46368.1"
                     /translation="MSEDRILLADMVFHGRHGTLQAEHELGQPFVVSVELHLDLRPAG
                     TSDDLTKTVDYGEIHRMARDIVEGPSVQLTETLAERIAARTLDEHPAVEAVKVKVAKP
                     HVRLEDTVLAGSAVEILRRREARRANYGVSS"
     gene            5115..5843
                     /locus_tag="VNT12_09375"
     CDS             5115..5843
                     /locus_tag="VNT12_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HWI46369.1"
                     /translation="MVMGEKQHPSAGGEAPTRPSVTVSYAQTLDGRVATSTGESKWIS
                     CPDSLRFAHEMRASHDAVLVGAGTVCKDDPRLTVRHVPGTSPLRVVVDSTLRTPPNAA
                     VLADGAAQGTVFAVTDRAPEERCERAYSLGATIMKLPSSLDGRVDLAALLEGLVASGI
                     GSVMVEGGATLVTGFFRARLVDRIAVCVAPKILGEGIEAIGDLGIRNLSRSLTLADVA
                     VEPYGVDLILSGRIEYPDGVPDRG"
     gene            5821..6873
                     /locus_tag="VNT12_09380"
     CDS             5821..6873
                     /locus_tag="VNT12_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HWI46370.1"
                     /translation="MVSRIGDELKARALWFTAPRTAALRAETVEPPGPGEVRVETIAS
                     AVSAGTEMLVYRGEVPRDLRLDLPTLAGSYGLPIKYGYAAAGRVLDAGSGVEHLSPGD
                     RVFVHHPHQDVFVVPAQMPVLLPDDLDPVLGVFSANLETALNIVHDTPVRLGETALIF
                     GQGIVGLLVALLLQLAGAGPVLVVDPLEERRRLALAAGADGAFAPGGLSDRVMEITGG
                     RGADVAVETSGSGAALQSAIDAVATEGTVVVASWYGTKPVTLALGGHFHRGRVRLRSS
                     QVGRMNPELAPRWDRARRMDTVLGLLGRLKLRELISHLIPFGEAPEAYTLLDERPEEA
                     LQVIFIYEGLRGDQDV"
     gene            6866..7243
                     /locus_tag="VNT12_09385"
     CDS             6866..7243
                     /locus_tag="VNT12_09385"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWI46371.1"
                     /translation="MYEIYVAARFEAVHRLVGDFGPATRTHGHTYRMEVIVRGKHLGD
                     DGTLYDIGGLGQAVEGLAGSMHYRDLNEVPGLEGVNTTAEAVASYCWDKLAEPLRGQG
                     LDSLTVRIWESPDAYAAREDALA"
BASE COUNT         1389 a         2149 c         2400 g         1310 t
ORIGIN      
        1 ccgcaagggc atcgaggacc tgagccactg gtatcccgga gcggacttca ggccactcgc
       61 gctcttccgc atcctttatg gacagtttgc ccagccgcct ggcattcaca gcagtctgtc
      121 ggagaacatg acacagacgc cggggctcgc gggagagtac accgaggatt cttccataag
      181 cggttccatg ctttcgggag agaaggctgc cggggcggtg atcgcgtgag cgccaacggc
      241 actccggcgc taaagataga gaagctcacg aagacctatt ccaacggttt tctggccctt
      301 gacggggtct cgctcgaagt ggaggccgga caattcttcg ggttgctagg gcccaacggt
      361 gcaggaaaga cgacgctcat aaatggcgtg gtcagccttg ccaggcctga ttcgggctcg
      421 gtcgaggttt ttgggaagga tgcctataga gagtttcggg aggcgcggcg tatgatcggg
      481 gtctcccccc aggaagttaa cctggacaag ttcctgaccg tcgaggagac attgcttttc
      541 cacgcaggat actacggcgt tcccaggaag aaagcccgtg agagggccga ggagcttcta
      601 gagcggttcg agctcacagg gaagcgcgga cagaggacca acacgctctc gggagggatg
      661 aagcggcgcg tgctgttcgc cagggcgctc atgcacgacc cgaaggtgct gtttctggac
      721 gagccgacgg ctggcgtaga cgtggagtta cgctacaagc tgtggggcta cataagggag
      781 ctaaacggtg gggggatgac tatcctgttg acgacgcact atctggagga ggccgaggcg
      841 ttgtgcgagg agatcgcact cataaacggg ggtaacatag cagcccaggg cagcagcgaa
      901 gatctcaagg agcgctacga ggctaggaac gtcgaggaag tctacatgaa ggtgataggc
      961 catgatcttc acggatagga ccttccagac acttctctcg cgcgagatac tgcgctttac
     1021 gcgggtctgg atgcagacga tagtcccgca gcttttgacc tcgcttctct atctcgtggt
     1081 cttcgggatc gcactcggcg gccgtataaa gcagatcgaa ggcatcccct acctcgatta
     1141 catattgcct ggcatagcct tgatgagcct gatcaccaac tcgcagatga actcgtcctg
     1201 gtcggtcttc gacgccaagc gcgagcgcta catagacgag gtgctcatca gcccgatgag
     1261 cgacctgcag atcacgctcg cctactcttt gggcggtatg ctgcgcggac tggtgatggg
     1321 ggccggggtc ttcatcgtcg gggtgccttt cgtcggggta cagatcaaac atccgcttat
     1381 gctggtggtg atcgggcttc tcgcctcctt cgcgttctcc tcttttggaa cgatggtcgg
     1441 cgccctggcg acccggatag agcacatctc actcatcacg agcgtcatca tccagcccct
     1501 tgcgttcctg ggaggggtgt tctactccgt aggtatgttg cccggggtgc tcaaggtggt
     1561 gacgttcttc aaccctatct tctacaccgt cgacgcggcc cgctacgtaa cgctcggcgt
     1621 ctccgatctg aacccctacc cgaccctggg tatggtctgc cttatagcca ccatctgctt
     1681 tgggggcgca tggtgggcga taagccgggg cccgaatcta cgatactgag tcggccaagg
     1741 tagggggctt cccaatctac gcgctgtcaa tgttacgcgc aaacagggtc tggccagtac
     1801 gaggaggacc cggaataggg ccctacatct cgctcgagaa tctcgaaacg catcagcgcc
     1861 gcgacctgct ggcttccagc acgagaagcg ctatcaggac gatctcgatg atcttgtcgg
     1921 tgtagcctat gacagtgcgc gccccgacca ctatccagag gaagatggta agcgccgtgt
     1981 agccgaggag gatccaccgt gtgaggttcc tgtaatccga gatccgtggt atcggtaaaa
     2041 tcagtgccgc gaggagcgta agatacccca gcccgttgag gacgaagagc gggaagccct
     2101 ggaggccaag gtagaggtgg atcagggccg tcccgatggt caggacgatc actcctaccc
     2161 ttagcgggtc gttagtggag acgttcgtag aggctatgct cttcctcctt tccggcctgc
     2221 gcctgccctg atatcttcgc cctctgtaca acccttgtcc ttcgccctgg cagtagatca
     2281 gccgctgaga ctatcagggc gaggataatg agcgccccgc cgatgagata aggtgcaggg
     2341 atgccgagat ggtcgaaggc gagcccggct gtcgcggggc cgaggatcat ggcgaggctg
     2401 aggatcgcct gcagacctcc cataagcctg ccctgtgtct cggccggggt ccggttggag
     2461 atgatggctg tgaggctcgg gatggcgagc cccatgccga gcgccaggag gccgacgacc
     2521 gggtagagga ggacaccgga agggaccagt gccacgagga ggaggttgag ggagacaagg
     2581 acgagtccgc cgacgaggag tctgctctcc ccgaagcgcg gctggaggcg tccgatcaag
     2641 aacccctgcg tgatgacggc gcacactccc acgaaagcga agaagaaggc gtttgccgtg
     2701 gtgccccacc caaacctgac ctggctgaag agcgggaagt tattcacgag cccggcgagg
     2761 gctaggttga gcagcagcac tgctgcgagc aaggcccgaa tgtgtctcat cttgagtatg
     2821 ccggccagtt gtgagatggg gtcgagacgc aagaggggga ccttccggcg caggtgggac
     2881 gcaagggact cggggagggt caggtagccg aagacgcagt tggtgagagc cagtgctgag
     2941 gcaaagaggg ctggtgcgct caatgagtgg aggctaagga ggccgccaag ggccggtcct
     3001 gccatgaggc cgagcccgaa ggcagccccg atcagaccca gtccccgcgc cctgtcctcc
     3061 ttggccgtgc tgtcggctat gtaggcctgg gcggtcgcca gcgtgccgct cgccgcgcct
     3121 gccacaacga cggcgaggac gagagaggcg agtgtctgag cgaggccgag cagcaggtag
     3181 gcgattgagg tcccgagcag gcaaagcagg agaacagggc gtctcccatg acggtccgac
     3241 agcccgccga ggaagggacc gccgacaaac tgcattgcgg cgtacaggga accgagcagg
     3301 ccgacgagca ctgcgccgga agcatacggg ccagcgtaga atggtagcaa ggggaccacg
     3361 atgccgtacc cgatcatgtc cacgaacacc gtgacgaaca ggaatacgag cggcgaccgg
     3421 cgacgccaca cgtccgacgt cacgcggtct ccctattctc aatcaaccgc cgcgtctgct
     3481 cgatcaccgc gtcgccgacg gctcccggtt cggcatggat gggacttccg aactcgacca
     3541 tggcgtccac attgcgcagg gccggaacaa gcacctggag cgtggcggcg agccactcgc
     3601 ggtcgcgcct gcggcggcgc aagtggatca gggggttccg cagggcagaa ggtgagatca
     3661 caccgctgac cacgacgggg acgaccacaa gccctggtgt gaggcgagcg aggaggtcgg
     3721 cgctcgaaga ccagtgattc agggcctcga cggcgcctgg caagaccgca gggtcgggct
     3781 cgatcctacc ccctggaaac gtgagtacgg cgccgccgcc ccgcaggtgc cgggccgccg
     3841 cccgcacgac gcccgagcgt cccgcaaagg tctcggtgac agtcaaaagg tgacgcgatg
     3901 tattcgggag gacttctaga aagggacggt cggcggcgat gacacgcagg tcgtccctcg
     3961 gtatcgtcgc gaagagggcg acggcgtctg cgagaccagg atggttggag acgaggagca
     4021 gcggcccgcc tcgcggtaca ttctcctgac cctcgatgct ggtgttgcgg gaaaatctct
     4081 tcagcgccca cgcgcctccc gtcccgagcc cagtctcgcc gactattgtg tcgtaggtca
     4141 ggatctggtg cgccagacgc cgagccggta tgcgcgcgat agattccatt accgcgcgcc
     4201 cgtgactcgt tccgcccagg ccgaaggcgg agataagatc gtccacgcat agctgcgtga
     4261 gccgttcgag atgaccctca cacctcgaag aagcgtggag ttcctcggtt gctgtcggca
     4321 gttgggagac gttatccaaa gcgcttgcct gcgaccctat ctataaggtc aatgccaggt
     4381 aggacggtat ccgtgagcct tgtggtctct acgtgtgcca ccctttcgcc tgccccaagc
     4441 gatgatcggg gcgccttcac ctcatctgtt agtacgcacg ggccgcaggt gcggttcggg
     4501 taccgctaag aggatacccc atagtttgcg cgcctagctt cgcggcggcg taggatctcg
     4561 acagcagagc cggcgagtac ggtgtcctcg agcctgacgt gaggcttggc caccttcacc
     4621 tttacggctt cgaccgcggg atgttcgtcg agcgtgcgtg cggcgatcct ctccgccagg
     4681 gtctcggtga gttgcaccga aggtccctcg acgatgtcgc gcgccatgcg gtggatctcg
     4741 ccatagtcca cggtcttcgt caggtcgtcg gaggtccccg ctggtcgcag gtcgaggtgc
     4801 agctcgacgc tcacgacaaa aggctggcca agctcgtgct cggcctgcag cgttccgtgg
     4861 cggccgtgga agaccatgtc cgcaagaagt atgcgatcct cgctcatcgc ttcttttcgc
     4921 tcccttccat ccgaatcagt atgccagaac gtaaagaccg gtatgacgcg tatgcggtta
     4981 gggagaaaag tcggctatag tagagtgagg tacctgcaga cgaaagagga gaagatgggg
     5041 cacctcttcc aagcatcgat cttacccgga ggcatacctg acgaatggct cgcaggtagt
     5101 caggcgagca gggcatggtg atgggagaga agcagcatcc ttcggccggc ggagaggctc
     5161 cgacccgacc ttctgtcacc gtgagctacg cgcagacgct cgacgggcgc gtggcgacca
     5221 gcacggggga atcgaagtgg atcagctgcc ctgactccct gcgcttcgcc cacgagatgc
     5281 gcgcctctca cgacgccgta ctcgtcggcg ccggaacggt ctgcaaggac gatccccgtc
     5341 tgacggttcg acacgtccca ggcactagcc cactgcgcgt ggtcgttgac agtaccctgc
     5401 gcacaccccc caacgccgcc gtgctcgctg atggcgcggc ccagggaacg gtgttcgctg
     5461 ttacggaccg cgcgccggaa gaacgctgcg agagggccta ctctctgggt gccaccatca
     5521 tgaagctgcc ttctagtctc gatggtcgtg tcgacctcgc ggctctcctg gaggggttgg
     5581 tagcgtcagg catcgggtcg gtgatggtcg agggaggggc caccttggtg acgggctttt
     5641 tcagggccag gctggtcgac cggattgccg tgtgcgtcgc gcccaagatc ctgggtgaag
     5701 gcatagaagc tatcggcgac cttggtatac gcaacctgtc ccgatctctc accctcgccg
     5761 acgtcgcggt ggaaccttac ggggtggatc ttatcctcag cggacgcatc gaatacccgg
     5821 atggtgtccc ggatcgggga tgaactgaag gccagggccc tgtggtttac cgccccacgc
     5881 accgccgcgc tccgcgcgga gacggtagaa cctcccggac ccggcgaggt acgcgtcgag
     5941 accatcgctt cggccgtgag cgcgggcacg gagatgctgg tctatcgcgg agaggtaccg
     6001 cgggatctcc gattggacct gcccaccctc gcagggagtt acgggctccc gatcaaatac
     6061 ggctacgccg ccgcagggcg tgtcctcgac gcgggttctg gcgtcgaaca cctttctccg
     6121 ggagatcgcg tcttcgtcca ccatccgcac caggatgttt tcgtcgttcc cgcacagatg
     6181 cccgttctac tccccgacga cctagacccc gtgctcggcg tattcagcgc caacctcgag
     6241 acggcgctga acatcgtaca cgatacgccc gtacgactcg gcgagacagc cctgatcttc
     6301 ggccagggta tcgtcggttt gctcgtagcc ctcctcctgc agcttgccgg cgcgggacct
     6361 gtactcgtag tcgaccctct cgaggagcgt cgcaggctgg cgcttgccgc gggcgctgac
     6421 ggcgcgtttg cgccgggagg gttgagtgat cgtgtcatgg aaatcacggg tggacggggg
     6481 gctgacgttg cggtcgagac tagcgggtcc ggggctgctc tgcagagcgc gatagacgct
     6541 gtggcgaccg aggggacagt cgtcgtggct tcgtggtacg gcaccaagcc cgtcacgctc
     6601 gccctggggg gacatttcca taggggaagg gtgagactca gatcctcaca ggtcggtcgt
     6661 atgaatccgg agcttgcgcc gcgctgggac agggcccggc gcatggacac ggttctcggc
     6721 ctgctgggcc ggctgaagtt gagggagctc atctctcacc tcatcccgtt cggagaggcg
     6781 cctgaggctt acacgctcct cgacgaaagg ccagaagagg cgctgcaggt gatcttcatc
     6841 tacgagggac tccgaggaga ccaggatgta tgaaatctac gtcgccgcga ggttcgaagc
     6901 tgtccacagg ctcgtagggg acttcggtcc tgcgaccagg acgcacggac atacctacag
     6961 gatggaggtg atcgtgcgcg gcaagcatct cggagacgac ggcacgctct acgacatcgg
     7021 gggactgggg caggctgtag agggactggc cggctccatg cactacaggg atctgaacga
     7081 ggtcccaggc ctggagggag tcaacacgac ggccgaggcg gtggcctcct actgttggga
     7141 taagctggca gaacccctcc gcggccaggg actcgactcg cttacggtgc gcatctggga
     7201 gtcccctgac gcttatgcag cccgcgaaga cgcgctggcc tgaaagga
//
LOCUS       DATMJA010000393         5563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_526131, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000393 DATMJA010000000
VERSION     DATMJA010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526131"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..768
                     /locus_tag="VNT12_09390"
     CDS             199..768
                     /locus_tag="VNT12_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46372.1"
                     /translation="MRYYSNGKPSAKAPDGSKVILSGKGAWNPKKDIAKGGGHYAIKG
                     SSGAIKAQGSWRVTDFRAFEQFSGWWGMGPNFKEKGWQGPPGSSSFSGFLTLKVSLEN
                     QGEGTLVAWCLMPQVLKKHPHLANPDGHVGDGISLTGGKFNFMNFKENEMSLEGVMFY
                     STDPASGGYVLTPKGTTVPRGAPDDEDHD"
     gene            complement(771..1472)
                     /locus_tag="VNT12_09395"
     CDS             complement(771..1472)
                     /locus_tag="VNT12_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI46373.1"
                     /translation="MGEADAWQRRVEEFVLGQIGDSPTRVLEVGCGEGELARTLARVG
                     HSVTAIDPQAPEDPIFRRTRIEEFTDPAQFDHVVAILSLHHVEDLGMAVSNMADLLRA
                     GGTLVVVEFAWDRIDEATAEWALERLPVASLSEKHSWLGRRCQEWARGGRGETRAPAA
                     ESYFAEWASEEGFHSSLLIRDELGRRFVERFFEWVPYLYPELREDVSESDESAAIEAG
                     TINSTGFRYVGTRLA"
     gene            1662..2330
                     /locus_tag="VNT12_09400"
     CDS             1662..2330
                     /locus_tag="VNT12_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWI46374.1"
                     /translation="MDAGTHREFKNRLFEQFARVGKALASPKRLEIVDLLAQGERTVE
                     EIARETAMSVASASQHLQALKTARMVEVRREGLYAHYRLADEDVFRTWQAVRALAESR
                     LSEVDRVVEAYLVDRDDLEAVDATELMERLSNGNVVVLDVRPEEEYRAGHIPGALSVP
                     VDTLEAALQTLPRDREIVAYCRGPYCVFSDEAVALLRSRGYRAHRLRQGLPDWRAAGM
                     PVEG"
     gene            2345..2734
                     /locus_tag="VNT12_09405"
     CDS             2345..2734
                     /locus_tag="VNT12_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI46375.1"
                     /translation="MTKGNIEAMTLRDMLERGERVTVVDVRKGEDHAEWSIPGSVHVD
                     ANDALNSGDERAMEGLELPEGAPVVTVCGRGRSSAVAAEQLRRQGYEALSLEGGMKAW
                     SLVWNTAEVPLPGVSAEVVQMRRTGKG"
     gene            2750..3454
                     /locus_tag="VNT12_09410"
                     /pseudo
     CDS             2750..3454
                     /locus_tag="VNT12_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            3485..4732
                     /locus_tag="VNT12_09415"
     CDS             3485..4732
                     /locus_tag="VNT12_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI46376.1"
                     /translation="MSPTIQLGLKENLGQFSLLVLVNAFVGGMVGLERTVVPLIGSQE
                     FGLVLRTAIFSFIVTFGLIKAFSNLFAGGMADSFGRKKVLVAGWLVGVPVPLILMYAP
                     SWEWIVAANVLLGVNQGLAWSMTVIMKIDLVGPRGRGLAVGLNEFAGYLAVGLTALAT
                     GYLASLYGLRPEPFYLGVGYALLGLLVSVFLVRDTGEHIRLEMEEHPPEPSPLTLREV
                     FAKTTYGDRNLFACSQAGLVNNLNDGMSWGVYPLFFANFGLGVGRIGILKAVYPAVWG
                     TLQVATGPLSDRWGRKGLIVAGMWVQAAGIFLTVAMRDFGWWVLGSVLLGLGTAMVYP
                     SLIAAVSDASHPSWRARSLSVYRFWRDLGYAVGALSAGVIADTLGVSWAIGSVGALTF
                     LSGAIVAVAMREAAEGRVGSEKG"
     gene            4740..5321
                     /locus_tag="VNT12_09420"
     CDS             4740..5321
                     /locus_tag="VNT12_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWI46377.1"
                     /translation="MKNMRSKDVQRFFEQVATDWDTMRLAYYDERVIEKMAEISGVGK
                     DSEVADVGTGTGFVAAGIAPRVKRVVGIDYVPAMLEVARENLATLGVSNVNLVVGEAT
                     RLPLEDGSVDAVFANMVLHHAEHPTAMLLEMARVVRLGGSVVITDEVEHPYAWMREEH
                     ADVWLGFGRGQVEHFFAAAGLSEGYGYEPLGMQ"
BASE COUNT         1005 a         1699 c         1856 g         1003 t
ORIGIN      
        1 acacgagaga ggcgggcggg gcgaaggagg ctgctggccg tctccgctgc gctggttatg
       61 gcgctggcag cactgttgct caccctggcg ctcagtagca gcatagggcc gtccaacgcg
      121 gcggtccaat aagagctgcc ctatactttc ttcttcactc actacgaact gcttggcgat
      181 ccgaacaacc cagatggggt gaggtattac tccaatggca aaccctctgc caaagcgcct
      241 gatggctcga aggtcatact ctcgggtaag ggcgcttgga accccaagaa ggacatcgcc
      301 aagggcggcg gtcactatgc catcaagggc tcctcgggag ccatcaaagc acagggatca
      361 tggcgagtaa ccgacttcag agcgttcgag caattcagtg gttggtgggg gatgggacca
      421 aatttcaagg agaaagggtg gcagggacct cctggatcgt cttccttctc tgggtttctg
      481 acgctcaagg tgagccttga gaaccaggga gagggcaccc tggttgcctg gtgtctgatg
      541 ccccaagtcc tcaagaagca cccacacctt gcaaatccgg acggtcatgt gggagatggc
      601 atctcgctta cggggggtaa gttcaacttc atgaacttca aagaaaatga gatgagcttg
      661 gagggcgtga tgttctattc gacggaccca gcgagcgggg gatatgtgtt gactcccaaa
      721 gggactaccg ttcccagggg ggcgcctgac gacgaggatc acgactaacc ctatgctagc
      781 ctggtaccca cgtagcgaaa cccggtggaa ttgatggttc ccgcctctat ggccgcgctc
      841 tcgtctgact ccgatacatc ctcacgcagt tccgggtaga ggtacgggac ccactcaaag
      901 aagcgttcca cgaagcgtcg cccgagttca tcgcgtatca gcagggagct gtggaacccc
      961 tcctcgctgg cccactcggc gaagtacgac tccgccgcgg gagcccgtgt ttctccccgg
     1021 cccccacgcg cccactcctg gcagcggcgc cccaaccacg aatgcttctc tgacagggag
     1081 gcgaccggaa ggcgctctag ggcccactcc gccgtggcct cgtcgatccg gtcccaggcg
     1141 aactccacca cgaccagggt cccccccgcg cggagcagat cggccatgtt gcttacggcc
     1201 ataccgaggt cttcgacgtg gtgtagggac aggatcgcca cgacgtggtc gaactgagcg
     1261 ggatctgtga actcctcgat tctcgttcgc cggaagatgg gatcttcggg ggcttgtggg
     1321 tcgatcgcgg tcacggagtg gccaacacgc gcgagggttc gggccaactc tccctcccca
     1381 caaccgacct ctagcacccg tgtaggggaa tcaccgatct ggccgaggac aaactcctcg
     1441 acgcgccgct gccacgcgtc tgcctcaccc atgatagcgg gtgctcaccc tagaaaagac
     1501 ggctacgtac gcggcgctgt tcactatcgg accattctgc cacgaacggg cagcttctta
     1561 gaaccctctc ggtatgacgc tcgcaggaca ctccctctac acactcggat gaacagatcc
     1621 atcttgacgc ccaggttcta ttcaactaat gtgttgaata tatggacgct ggcacccaca
     1681 gggagttcaa gaaccgcctc ttcgagcagt tcgccagggt gggcaaggct ctggctagcc
     1741 ccaagcgcct ggagatagtg gacctccttg cccaagggga gcgtacggtc gaggagatcg
     1801 ccagggagac ggccatgtcc gtggccagcg cctcgcagca cctccaggcg ctcaaaacgg
     1861 cgcggatggt cgaggtgcgc cgcgaggggc tgtacgcgca ctaccggctg gctgacgaag
     1921 acgtgttccg gacatggcag gccgtccgcg ccctggcgga gtcgcgcctg tccgaggtcg
     1981 acagggtggt agaggcctac ctggtggacc gggatgactt ggaggctgtg gacgccacgg
     2041 agttgatgga gcgactgagc aacgggaacg tggttgttct cgacgttcgt ccggaagagg
     2101 agtaccgggc cgggcacata cccggagctc tctccgtgcc cgtagacacc ctagaagctg
     2161 cgctgcagac cctcccacga gaccgggaga ttgtcgccta ctgccgtggg ccctactgcg
     2221 tgttctccga cgaggcggtg gcactcctca ggtcgcgcgg ctaccgggca cacaggttac
     2281 gacaagggtt gcccgactgg cgggcggccg ggatgcccgt ggagggctag aaagggagtc
     2341 gaacgtgact aaaggcaaca tcgaggcgat gaccttgagg gacatgctgg agcgcggcga
     2401 gcgggtcacg gtcgtggacg tgcgtaaggg agaggaccac gcagagtggt cgatacccgg
     2461 aagcgtgcac gtcgacgcca atgatgccct gaactccggc gacgagcggg ccatggaggg
     2521 gcttgagctg ccagaagggg cgccagtggt aaccgtgtgc gggcgtggcc gttcgagcgc
     2581 ggtggccgcg gagcagttgc ggcgtcaggg atacgaggcg ctctccctgg agggcggaat
     2641 gaaggcctgg agcctcgtct ggaacacggc cgaggtgccc ctgcccggag tgagtgccga
     2701 ggtagtgcag atgcgtcgca ccgggaaggg gtgactctcc tacgtcgtcg ggtccgacgg
     2761 caaggccgta gtcgtagacg cctccctgga accggaggtg tacctctggt tggccgaagg
     2821 acgcggctgg cggataaccc aggtgctcga tacccacgtc cacgccgacc acctttcccg
     2881 ctctaggagg ctggccgagg tggtcggtgc gaccctccac atgcccgagg gtgcttcggt
     2941 ctcctacccg atctctgctc tcggggacgg ggacgtgctc caggtcggct cggcgtcgct
     3001 ggaagccgtc tggacccccg ggcacacccc ggagagcacg agctacctgc tggacggtcg
     3061 ggccgtcttc agcggggata cgctcttcgt gtcggcggtg ggccgtcccg acctggacac
     3121 aacgccggaa ggctcccggg agaaggcgcg agagctctac cgctccctgc agcgcctact
     3181 tgccctcggc cccgagactc ttgtcctgcc cggacacacg agcaagcccg tcgcgttcga
     3241 cggtgaaccg atctacgcgc tgctgtcgga agtgcaccag agtgtccgga tcctgcggga
     3301 ggcccaggac gccttcgtgg aagagatcgc gggccacgtc tcgcccacgc cttcgaactt
     3361 cgagcgcatc atcgaactta accgagcggg agtatcaccg gaaggcgacc ccacagagct
     3421 ggaggccggc gcgaaccgct gcgcggcggg ctagggttcg gtgcccgctg aacgggtacc
     3481 ctcggtgtcc cctacaatcc agctcggcct gaaggagaac ctgggacagt tctcgctgct
     3541 ggtcctggtg aacgccttcg tgggggggat ggtgggtcta gagcgcacgg tggtgcctct
     3601 gatcggctca caggagttcg ggctggtcct caggacggcc atcttctcgt tcatcgttac
     3661 cttcgggctc atcaaggcat tctccaacct cttcgccggg gggatggccg acagcttcgg
     3721 caggaagaag gtcctggtag cgggctggct ggtgggcgtc ccggtgcccc tcatcctcat
     3781 gtacgccccc tcctgggagt ggatcgtagc tgccaacgtt ctgctcggcg taaaccaggg
     3841 cctcgcgtgg tctatgactg tcatcatgaa gattgacctc gtgggaccac gcgggcgggg
     3901 ccttgcggta ggcctcaacg agttcgccgg ctacctcgcc gtgggactca ccgcgctcgc
     3961 caccggctac ctcgcctccc tctacgggct cagaccggag cccttctatc ttggcgtcgg
     4021 ctacgctttg ctcgggctcc tagtctccgt cttcctcgtg cgcgacacag gggagcacat
     4081 ccgcctggag atggaggagc acccaccgga gccgagcccc ctgacgctcc gagaggtgtt
     4141 cgcgaagact acctacgggg accgcaacct cttcgcctgc tcccaggcgg ggctcgtcaa
     4201 caacctcaac gacggcatga gctggggcgt ctatcccctg ttcttcgcga acttcggcct
     4261 cggcgtgggc cgaataggca tcctcaaagc cgtctacccg gcggtctggg gaaccctgca
     4321 ggttgcgacc ggccccctta gcgaccggtg gggccgcaag ggcttgatcg tggcggggat
     4381 gtgggtccag gcggctggaa tcttcctcac cgtggcgatg agggacttcg ggtggtgggt
     4441 actcgggagc gtcctacttg ggcttggcac ggcaatggtc tacccttctc tgatcgccgc
     4501 cgtctcggac gcttcccacc cctcgtggcg ggcacgctcg ttgagcgtct accgcttctg
     4561 gcgggatctc ggctacgcag tgggcgccct ttcagccggg gtaatcgcgg ataccctggg
     4621 ggtctcgtgg gccataggct ctgtgggggc gttgacgttc ctctccgggg cgatcgtggc
     4681 ggtcgccatg cgggaggcag cagaaggccg cgtcgggagc gagaaaggat gatcggagca
     4741 tgaagaacat gagatctaag gacgtgcaga gattcttcga gcaggtggcc accgactggg
     4801 acaccatgcg cctcgcctac tacgacgagc gcgtgatcga gaagatggcc gagatctccg
     4861 gggtgggtaa ggattcggag gtcgcagacg tcggcaccgg aacgggattt gtcgccgccg
     4921 ggatcgcccc gcgggtaaag cgcgtcgtag ggatagacta cgtcccggcg atgctggagg
     4981 tggcccgaga gaacctcgcg accttgggcg tatcgaatgt gaacctcgtg gtcggtgagg
     5041 ccacgcggct gcccctggag gacggctcgg tggacgcagt gttcgccaat atggtcttgc
     5101 accacgccga gcaccctacc gccatgctcc tagagatggc ccgagtcgtg agactcggag
     5161 gcagcgtcgt cattaccgac gaggtggagc acccctacgc gtggatgcgc gaagagcacg
     5221 cagacgtctg gctcggattc gggcgggggc aggtggagca cttcttcgcc gcggcggggc
     5281 tgtccgaagg ctacggctac gagccgctcg gcatgcaatg atgcatccgt tctacgacct
     5341 cgggtgaggt cgctgacatc ggcatcttcg ccgcgtgggg cacggtgccg cgccgttgag
     5401 tggaactcgc gatcgcgagg taggatcaca tgcggtggtt gtcctggagt atgcgaacct
     5461 cggaggagac cctacacccc aacttcggtt agagcattgt tcttgcggag agtattcggc
     5521 ttatctatgg gaaaaagaaa gagccgacga gcggactcga acc
//
LOCUS       DATMJA010000394         3956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_822661, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000394 DATMJA010000000
VERSION     DATMJA010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822661"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VNT12_09425"
     CDS             <1..638
                     /locus_tag="VNT12_09425"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46378.1"
                     /translation="GTLQSVVFSRYLVYYNVYGHHALRIPTVMFLRAVQDAIGAGKVT
                     FDELSELDDAEAFVLLENSAESSTSRLMKRLADRRPYHLALEFDERHPSYSSLVRLRD
                     DASWRRRVEEAWSRYLTRYRKGVAGPFDILIDLPLREHFPVGLRLIRRNPLPGERNPI
                     GWQNLSGMSEDEMTRYHAPLRRILIATANDDLVKSVRRHAEELFTIAEEVG"
     gene            complement(914..1366)
                     /locus_tag="VNT12_09430"
     CDS             complement(914..1366)
                     /locus_tag="VNT12_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46379.1"
                     /translation="MDLQRLYAGSFIDWEVVASSWNKRTVPSRLLLFAARRYLDERGD
                     NVDSGRYEVLASAPLPEEIKQAFASPPELDAPAPRHWGEFVDAAVATELEMVSYGERP
                     PLLHELRAGLEQAAAEAGPLTEKGRWFLARRDALPGTDLPDDPGYSPI"
     gene            complement(1424..3394)
                     /locus_tag="VNT12_09435"
     CDS             complement(1424..3394)
                     /locus_tag="VNT12_09435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWI46380.1"
                     /translation="MNEFFETSKKKSKRRPRGRWVQWGLRALFNLVLVLGVSAAVGIA
                     CLYLFVLREYENDLDRTYPELAENSYVYDVDGNKIGEIPVTESRETVGFEGLGAYLPE
                     AVIAVEDRRFYDHFGVDFEGLARAALTDLRSWEVQEGGSTITEQLMKNLYVPEGQRLE
                     TSFRRRFVQSALAFAYERKHTKNEVLTAYLNTVYFGDGAYGAEEAAQRFFGKSARDLD
                     LSESATIAGLLHAPSTYMTWEGDVIEGQVRERRDSVLRQMQEQGMISSREREEAEDQP
                     LKFAPDPPPEDPAYTPFLEKVGREVEEELSPRALQLGGLRIYTTMDPDLQRAAVETSK
                     DVLSEPDDPSAAVVTIEPQSGAIRSLAGQDGDFNLPLDARRQPGSSFKPVVLAAAIKE
                     DISPQSTYISQDLAFDFQDEYYEIHNYDHVERGEISISDAMAESDNTVFVQLAADVGL
                     EDVVKTAEDLGITTPIEPYPSTAIGGLGSGVSPLDMASAYSTFAGGGIHREPYSIERI
                     DRNSYGQSETVYDHQISGRRVLTGNEAAVATQVLRGVVEDGTATMFHDLDEEIGRPSA
                     GKTGTTDDFADAWYVGYTPLRCTSVWVGYAEGRRSLVGVHGLQEPNGETLPMDIWSGY
                     MASATAGDLALEFPEADTGELYAVEGGYSGGF"
     gene            3481..3789
                     /locus_tag="VNT12_09440"
     CDS             3481..3789
                     /locus_tag="VNT12_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46381.1"
                     /translation="MPLLSCTVRRVGDHESRTEEYEVAYVVGRATPHVLLIKYQAARA
                     YEINAALDAAVEVAREAMAEADPDQSGPTDEALSKFSWRTIQSAKYEQGASRSSSLAY
                     "
BASE COUNT          703 a         1270 c         1225 g          758 t
ORIGIN      
        1 cgggcacgct gcaatccgtc gtcttctccc gctacctggt gtactacaac gtctacggcc
       61 accacgccct gcgtattccg accgtgatgt tcctcagggc cgtccaggac gccatagggg
      121 ctggtaaggt gaccttcgac gagctctccg agctggatga cgcggaagcc ttcgtcctct
      181 tggagaattc agccgagagc tccacgtcca gactcatgaa acgtctcgca gaccgcaggc
      241 cataccacct cgccctggag ttcgacgagc gacaccccag ttactcctcg ctggtccggc
      301 tccgcgacga cgcctcctgg cgccgccgcg tggaagaggc ctggtctcgc tatctcaccc
      361 gctaccgcaa aggtgtagcc ggacccttcg acatcctcat agacctcccc ctaagagagc
      421 acttccccgt ggggttgcgc ctgatccgac gcaaccccct tcccggtgag cgcaatccca
      481 taggctggca gaacctctct ggtatgtccg aagacgaaat gacgcgctac cacgcccccc
      541 tccgacggat cctgatcgcc acagcgaacg acgacctcgt aaagtccgtg cgccggcacg
      601 ccgaagaact cttcaccatc gccgaggaag taggatagcg cgccgccttc ggcggcttgt
      661 cagcatttca gcacgcgccc tttcgggcga cttgtcagca cgcttcgggc ctttgcaggc
      721 cctcgcttct cagcatctca gctttctgcg ttctggctcc gttcggcagc gcacgagaag
      781 tgcactctgc cgcgctcggg ttctacgctc atggggaggt ggtgggatca ctaactgaaa
      841 tgctgagagc cgccgaccgc agggaggcgg cgggctgaca agcggaagcc gaaggctgga
      901 gcgtgctgaa accttagatt ggcgaatatc cgggatcgtc gggaaggtcg gttccgggga
      961 gggcgtcgcg cctggcgagg aaccatctgc ccttctcggt tagcgggcct gcctccgctg
     1021 cagcctgttc gagtccggcc cgcagctcat gcaagagcgg cggtctctcc ccgtatgaga
     1081 ccatctccag ctcagtggcg acggcggcgt cgacgaactc gccccagtgg cgcggggcgg
     1141 gcgcgtctag ttctggcggc gaggcgaagg cctgtttgat ctcctctggg agaggagccg
     1201 atgccagaac ctcgtaacgc ccggaatcaa cgttatcgcc tctttcgtcg aggtagcggc
     1261 gggcggcgaa gagcaataat cgcgagggaa cggtgcgttt gttccaggaa gaggcgacca
     1321 cctcccaatc tatgaaggat ccagcataca gcctctgcaa gtccatagaa tcattctagt
     1381 tcagcatttc aacatttcag cacctcagct tcgttgggag gtgctagaaa ccgccagagt
     1441 agccgccctc gacggcgtag agctcgcccg tgtcggcttc gggaaactcg agcgccaggt
     1501 ctccggccgt agcactggcc atgtaaccgg accagatatc catgggcagg gtctctccgt
     1561 tcggctcctg taagccgtgg acgccgacga gggagcgccg gccttccgca taaccgaccc
     1621 acacggaggt gcaacgaaga ggagtgtagc caacgtacca ggcatcagcg aagtcgtccg
     1681 tcgtccccgt cttacccgct gagggacgtc ctatctcttc gtccaggtcg tggaacatcg
     1741 tcgccgtccc gtcctccaca acgcccctga ggacctgggt tgcgaccgcc gcctcgttgc
     1801 ccgtgagcac gcgccgaccg ctgatctggt ggtcgtagac cgtctcgctc tgaccgtagc
     1861 tattcctgtc gattctctcg atggagtaag gctcgcggtg gataccacca ccggcgaacg
     1921 tcgagtaggc agaggccatg tcaagcgggc tgacgcctga ccccaggccg cctatggcgg
     1981 tcgacgggta gggctcgatg ggcgtggtaa tgcccaggtc ttcggcggtc ttcacgacgt
     2041 cttccaatcc cacgtcggcg gcaagctgca cgaacaccgt attatccgac tccgccatag
     2101 cgtcggagat ggagatctct ccccgctcga cgtggtcgta attgtgtatc tcgtagtact
     2161 cgtcctggaa gtcgaaggcc aggtcctggg atatgtaggt gctctgcggg gagatgtctt
     2221 ccttgatggc cgcggcgagc actacaggct tgaacgagct gccgggttgg cgcctggcgt
     2281 cgagtgggag gttgaagtcc ccatcctgtc cggccagcga tcttatggcg ccgctctgag
     2341 gctctatggt cacgacggcc gcagaggggt catccggctc cgagagcacg tccttcgagg
     2401 tctcgactgc cgcgcgctgg aggtcggggt ccatcgtcgt gtagatcctc agtcccccga
     2461 gctgcagcgc ccgcgggctt agctcctcct ccacctcgcg tccgaccttc tccaggaaag
     2521 gcgtgtaggc tggatcttcc ggcggcgggt caggggcgaa cttcaggggt tggtcctcgg
     2581 cctcctcgcg ctcccgggag gagatcatac cctgttcctg catctgcctc aggacgctgt
     2641 cgcggcgctc tcggacttgc ccctcgataa cgtcaccctc ccaggtcatg taggtcgaag
     2701 gggcgtgtag cagaccggcg atcgtcgccg actccgagag gtcgaggtcc cgggcgctct
     2761 tgccgaagaa gcgctgcgcc gcctcttctg cgccgtaggc gccgtccccg aagtacacgg
     2821 tgttcaggta ggcggtcagg acctcgttct tggtgtgctt gcgctcgtag gcgaaagcga
     2881 gcgcagactg cacaaagcgt ctgcgaaaag aggtttccag gcgttggccc tcgggaacgt
     2941 agaggttttt catgagctgc tcggtgatcg tggagccgcc ctcctgcacc tcccaggatc
     3001 tcaggtccgt caaggccgcg cgggcgagcc cttcgaagtc gacaccgaag tgatcgtaga
     3061 agcgccggtc ctcgacggcg atgacggcct cggggaggta cgcgccgaga ccttcgaaac
     3121 cgacggtctc gcggctctcg gtgacgggaa tctcccctat cttgttcccg tcaacgtcgt
     3181 agacgtaaga gttctcggcg agctcggggt aggtccggtc gaggtcgttc tcgtactctc
     3241 tcaggacgaa caggtagagg caggcaatgc ctaccgcggc ggatacgccg aggacgagaa
     3301 caaggttgaa gagcgcgcgt agtccccact gtacccagcg tccccgcggg cgcctcttgg
     3361 actttttctt gcttgtttcg aagaactcgt tcacgaccct ccggccgtag tctaccccaa
     3421 tcgtgtctgg ggtgatcgac gcggggcaag gtgcagctgt acggatggta cgatgcctgc
     3481 atgccactgt taagttgcac ggtcaggcgc gtcggcgacc acgagagccg cacggaggag
     3541 tacgaagtcg cctacgtggt agggcgggcg accccacacg tgttgctcat caagtaccag
     3601 gccgccaggg cctacgagat aaacgccgcc ctcgacgccg cggtggaggt ggccagggaa
     3661 gccatggccg aggctgaccc cgaccagagt gggcccacag acgaagccct ctccaagttc
     3721 tcctggcgta cgatccaatc tgccaagtac gaacagggcg cgtcccgttc gtccagcctc
     3781 gcctactgag agcaagtcag cacaccgagg ccttgcggcc ttcgcttgtc agcacggcag
     3841 ctctgccgcc ttgtcagcca ggtctcgcac gcgcgaatgc tgaagatcct acaagctgat
     3901 ggccctgtcg atcgtaagga tcaggccttc cgcatagcgc tttgagtagc ctggac
//
LOCUS       DATMJA010000395         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_348977, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000395 DATMJA010000000
VERSION     DATMJA010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348977"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VNT12_09445"
     CDS             complement(<1..1687)
                     /locus_tag="VNT12_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46382.1"
                     /translation="MRRPGERRWTEETVIHASGDPSVDFPMIVLKPKLRAPLPRPEQI
                     VRRGLLDLLHDALERKVSVISAPAGYGKTTLLAHWRQVEEAEVPFAWVSLDEQDDDPI
                     RLWRHLVEALRQVVPDEDFGAEVLTRMSAVGQSYVEATLPTLINEIAESPHRVLLVLD
                     DYQSISAGEVHESVAFFVEHLPENFHLVISSRSDPPLPLGRWRARGEMNEIRTEELAF
                     SEEEADCLLNEKMGLNIGPDDLFVLLERTEGWPAGIYLASLSLQDKEDKHAFIASFRG
                     SNRYIVGLLGEEVLAGLTEEVRQFLLETSVLRTMTGPLCDAVTGREGSAKLLRELARS
                     NLFVVSLDEQDEWYRYHHLFSELLLYELRSSRPELVPVLRGRASVWLEGGGFFEGAIR
                     QAIVAADYERVRLLITRHWYGYVSAGQTATVQRWLESLPEGPTAHDAALLLVRAWICI
                     LSGRSEDGKRLLTLAESIPYEGPLPDGTASVEAGVAMLRAVFGLGGVRNTLKAARRAV
                     ALEPDQLSQRAALIRFGLGSSLYLCGEASQARKHFEDVVDLTRTGQPLLQMVSL"
BASE COUNT          405 a          667 c          600 g          392 t
ORIGIN      
        1 ataggctaac catctgcaac aggggttggc ctgtccttgt caaatcaaca acatcttcaa
       61 agtgttttcg cgcctgcgac gcctctccgc agaggtacag gctggatccc agcccgaagc
      121 gtatcaacgc cgcccgctgc gaaagctggt cgggctccaa cgctactgcg cgccgggccg
      181 ccttcagtgt gttacggacg ccgccaaggc cgaagacggc tcgtagcata gccacgcccg
      241 cctcgaccga tgcggtcccg tcgggaagcg gaccctcgta agggatgctc tccgcgagtg
      301 tcaggagcct cttgccatcc tcgcttctgc cggagaggat gcatatccac gccctcacca
      361 agaggagcgc tgcgtcgtgc gcggtcggcc cttcgggcag cgactcgagc caccgttgaa
      421 ccgtcgccgt ctggccggca gacacgtatc cgtaccaatg gcgcgtgatc agcagtctca
      481 cacgctcata gtcagcggcc acgatggcct gccgaatcgc tccctcgaag aatcccccac
      541 cctctaacca cacgctcgcc cggccgcgca atacgggaac aagctcgggc ctgctgctcc
      601 tgagctcgta gagcagcaac tcggagaaga gatggtgata tcggtaccat tcatcctgct
      661 cgtccaggga aaccacgaac aggtttgagc gggcgagttc acgcaggagc ttggcggaac
      721 cctccctgcc cgttactgcg tcgcaaagtg gacctgtcat cgtcctcagt accgaggtct
      781 cgagcagaaa ctgcctaacc tcctcggtga gtcccgccag gacctcctct cccaacaggc
      841 cgacgatgta gcggttgctg cccctgaaag atgcgatgaa ggcgtgcttg tcctccttgt
      901 cctgcaagga gagggaggcg aggtagatgc ctgccggcca cccttcggtg cgctcgagca
      961 gtacaaagag gtcatcggga ccgatgttta gccccatctt ctcgttcaac aagcaatcgg
     1021 cttcctcctc ggagaaggcg agctcttcgg tgcggatctc gttcatctct cccctcgccc
     1081 gccaccgccc aagcggcagc ggcgggtcgg agcgactgga gatgacgagg tggaagttct
     1141 cggggaggtg ctcgacgaag aaggccacag actcgtggac ctcccccgcg cttatggact
     1201 ggtagtcatc gagcaccagc aagacccgat gtggcgactc ggcgatctcg tttatgagcg
     1261 tgggaagcgt tgcctcgacg taactctgtc ccacggcgct catccgcgtg agaacctcgg
     1321 ctccgaagtc ctcatctggc acgacttggc gcaacgcctc cactaagtgc ctccacagcc
     1381 tgatcggatc gtcgtcctgc tcgtcgaggg agacccaggc gaagggcacc tctgcttctt
     1441 ctacttgacg ccagtgggcc aggagcgtcg tcttcccgta gccggcaggg gcgcttatga
     1501 cgctgacctt gcgctctaag gcgtcgtgga ggaggtcgag caacccgcga cggacgatct
     1561 gctcgggacg cggcaacggc gcccgcaact tgggcttgag gacgatcatg ggaaaatcaa
     1621 cgcttgggtc gcccgaggcg tggataacgg tctcctccgt ccagcgccgc tctccagggc
     1681 gacgcacacc cacctcacgg ccgatggggc accactagcg gcagagtcgt tgtgccgcta
     1741 ggccgtcaga gcaaggaact tcttacgtcc tacctcatga cttttgatgc cttcggcagg
     1801 ccaggttttc atctcttgaa tgttcccatt ctcgaccggg atccactgga ttccgacaaa
     1861 cgcctattat cgcacaatcg acagcagaac cgaaagtcta aagatgcaac ctacctgagg
     1921 aagcccaagc gcgcggtgtt tcactggcta cgtgcattgc cattcgaaca aagtatacat
     1981 accgcgcaac tgctctcgag ctgcgagttc gaggtactac agatgtttgc ggccggcacg
     2041 gggagccggt gatcccgttg cctg
//
LOCUS       DATMJA010000396         3571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_585535, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000396 DATMJA010000000
VERSION     DATMJA010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585535"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VNT12_09450"
     CDS             <1..375
                     /locus_tag="VNT12_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HWI46383.1"
                     /translation="VLESFSVVAATGRQSRAAVTISHAEQGTFEAEAEGEGPVDAVFR
                     AIDAATNIKGRLTDFRIDAVTGGKDALGEVRDSVEFEGGDYAGRGLSQDVVEAAARAY
                     VRSVNVYTAGMVGARWDAQVAP"
     gene            351..1136
                     /locus_tag="VNT12_09455"
     CDS             351..1136
                     /locus_tag="VNT12_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46384.1"
                     /translation="MGRAGSAVAARGRREAAPPGYERLDAEVPLLYAPRLAGKATEVR
                     GYLTQGYRTLGRILSVEPPGIVAYLVAGENWNRAPRDNARPYPPGLPYFTRSVEPPAL
                     VLPEELSTVFRPRTAATLPLTVWHELAHAFLLGREVVRTPAWLGEFVPQAASAAVARR
                     VGLPLAEHLSRIDTEPGLTVRGFRGQAGAADQMSFQNLLLLLGAAALEEFGEGFLQSI
                     FHALWEEDEIVDGERAEELLGDALGPGGREWLVSRPEFQEEPL"
     gene            1133..2200
                     /gene="leuB"
                     /locus_tag="VNT12_09460"
     CDS             1133..2200
                     /gene="leuB"
                     /locus_tag="VNT12_09460"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWI46385.1"
                     /translation="MRDSFDILLLPGDGIGPEVVGAAREVIDAVAEHFGIEMRYEERR
                     IGGTAIREEGEPVSAETLEAAGASDSVLLGAVGHPDFDDAPVRPEAGLLRLRKSLGAF
                     ANLRPVAAIPALIGSSPLKHEVVDGVDVLIVRELTGGAYFGEKEEGEERASDLSVYTR
                     EEIERVARVAFEAAKRRKRRVTSVDKANVMATSRLWRRVVSDVAEDYPGVSLDHILVD
                     AAAMFLVQTPTRFDVVLTENLFGDILSDEAAMLPGSMGMLPSASLGRPGSPGIFEPVH
                     GSAPDIAGEGKANPYATILSAAMMFRHALGRPDVSEAIEQGVSVALETHFLTPDVGGK
                     KKTEEVTKAVSKWVAAGEGVA"
     gene            2197..>3571
                     /gene="cimA"
                     /locus_tag="VNT12_09465"
     CDS             2197..>3571
                     /gene="cimA"
                     /locus_tag="VNT12_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HWI46386.1"
                     /translation="MSIKLFDTTLRDGTQREGVSLTAEDKARIARELDSLGIHYIEAG
                     FPASNPKDLEFFEGFDDASLSHAKLVAFTRARRPKGRAEDDPAVTLLPSLSAPVACIV
                     AKSWKLHVEKVLRTTPEENLESVRDTLAYLAGAGKEVIFDVEHYFDGFRDDPAYALAV
                     LQAAAEAGATTLVLCDTNGGTLPTELKAVVARTVREFPDVEVGIHTHNDSGCGVANAL
                     VAVEAGATHVQGTINGIGERCGNCDLVTTIANLQLKMGLKVMDDERLRQLTEASNYVS
                     ELMNLTPDTHRPYVGRSAFAHKGGLHVDGISKDPATYEHVAPEAVGNVRRTPVGELSG
                     KNSIRTKARELGLEARDATQILGAIKQREYEGYHYEAADASLALLIGRTTGEDRPLFE
                     LETFRIISEKRAGGETMTEATIKLSVGGQRVISTAEGNGPVNALDRALRKAIGPHYPE
                     LKEIHLSN"
BASE COUNT          661 a         1117 c         1232 g          561 t
ORIGIN      
        1 gtcctggagt ctttcagtgt cgtcgccgcc acgggccgcc agagcagggc cgccgtcacc
       61 atctcccacg ccgaacaggg cacgttcgag gccgaggcag agggggaggg gcccgtcgac
      121 gcggtcttcc gcgccatcga cgccgcgacg aacataaagg gcaggttgac ggacttccgc
      181 atcgacgccg tgacgggcgg aaaggacgcc ctcggcgagg tcagggactc tgtagagttc
      241 gaggggggag attatgccgg acgcggcctc tcccaggacg tcgtcgaggc ggcggcgcgc
      301 gcctacgtgc ggtcagtcaa cgtctacacg gccggcatgg tcggtgccag gtgggacgcg
      361 caggtagcgc cgtagcagcc cgggggagac gggaggccgc gccaccgggt tacgaacgcc
      421 tggacgcgga ggtgcccctc ctctatgctc cgcggctggc cgggaaggcc acagaggttc
      481 gaggctatct cacgcagggc tacaggacgc tcgggcggat actctccgtc gagcctccag
      541 ggatcgtggc atacctggtg gccggcgaga actggaatag ggcgccgcgc gacaacgcac
      601 gcccctatcc gcctggactc ccctacttca cccgttcggt cgagcctcct gcccttgtgc
      661 tgccggaaga gctatccacc gtgttcaggc cgcgcaccgc ggccacgctg cccctgaccg
      721 tctggcacga gctggcacac gccttcttgc tcgggcggga ggtggtgagg accccggcgt
      781 ggttagggga gttcgtgccg caggccgcct ccgcagcggt cgcccgtagg gtcggtctgc
      841 ccctggcgga gcatctgtca cggatagata cggagccagg gttaaccgtg cgaggcttcc
      901 gcggacaggc tggtgcggca gatcagatgt cgtttcagaa tctgctgctc ctgctcggcg
      961 ctgcggcact agaggagttt ggagaaggct tcctgcagag catcttccac gcactgtggg
     1021 aagaagacga gatagtcgat ggggaacggg ccgaggagct gctaggagac gcattaggcc
     1081 caggcggaag ggagtggctg gtgtcgaggc ccgagttcca ggaggagccg ctatgagaga
     1141 ctcattcgac atactacttc ttcccggcga cggcatcgga cccgaggtgg tcggggccgc
     1201 gcgtgaggtc atcgacgcag tggctgagca cttcggcatc gaaatgcgct acgaggaacg
     1261 caggatcggc ggcacggcca tccgcgagga aggcgaacct gtctccgcgg agacgctcga
     1321 agccgccggc gcctccgact cggtcctgct cggcgcggtc gggcaccccg acttcgacga
     1381 cgctcccgtg cgcccggagg cagggctgct gaggctcagg aagagcctcg gggccttcgc
     1441 caacctgagg cccgtggccg ccataccggc gctcatcggc tcctcgcccc tcaagcacga
     1501 ggtcgtcgat ggcgtcgacg tgctcattgt cagggagctg acaggcggag cttacttcgg
     1561 ggagaaggaa gagggggagg aaagggccag cgacctcagt gtgtacacgc gggaggagat
     1621 agagcgggtc gcacgggtgg ctttcgaggc ggcgaagcgt cgcaaacggc gggtaacgag
     1681 cgtggacaag gcgaacgtca tggcgacgag ccggttgtgg cgcagggtcg tctctgacgt
     1741 ggccgaggat tatcctgggg tatccctcga ccacatcctc gtcgacgcgg cggcgatgtt
     1801 cctcgttcag accccgacga ggttcgacgt cgtgcttacc gagaacctct tcggggacat
     1861 cctctctgat gaggctgcga tgcttcccgg ctcgatgggg atgcttccga gcgcgtcttt
     1921 aggcaggcca ggctcccctg gtatcttcga gcccgtccat ggctcggccc ctgacatcgc
     1981 aggagagggg aaggccaacc cctacgcgac catcctctcc gcggcgatga tgttccgcca
     2041 cgctctggga cgccccgacg tgtccgaggc catcgagcag ggcgtctccg tcgccctgga
     2101 gacccatttc ctgactcccg acgtaggcgg caagaagaag accgaagaag tgactaaggc
     2161 cgtgagcaag tgggtcgcgg cgggagaggg tgtggcatga gcataaagct tttcgatacg
     2221 acgctacgtg atggtaccca gcgagagggc gttagcctga ccgccgagga caaggcccgc
     2281 atagcccgcg agctggactc tctggggatc cactacatcg aagcgggctt ccccgcctca
     2341 aaccccaagg acctggagtt cttcgagggc ttcgatgacg cttcgctctc ccacgcgaaa
     2401 ctcgtcgcct tcacacgggc ccggcgcccg aaaggcaggg ccgaggacga ccccgccgta
     2461 acgctcctac cctctctctc agcgcctgta gcctgcatcg tcgccaagag ctggaagctg
     2521 cacgtggaga aggtcttgcg gaccacgccc gaagagaatc tcgagtccgt gcgcgacacc
     2581 ctcgcctacc tggccggcgc cggtaaagag gtcatcttcg acgtcgagca ctatttcgac
     2641 ggcttccgcg acgatcccgc gtacgcgctc gcggtgctgc aggcagcggc agaggcgggg
     2701 gcgacgacgc tggttctctg tgacacgaac ggggggacgc tcccgacgga gctcaaagcg
     2761 gtcgtggcgc gcacggtgcg cgagttcccc gacgtagagg taggcatcca cacccacaac
     2821 gactcggggt gtggggtggc gaatgccctg gtggcggtag aggccggggc gacgcatgtg
     2881 caggggacca tcaacggtat cggggagcgg tgcggcaact gcgacctcgt cacgaccatc
     2941 gccaacttgc agctcaagat ggggctgaaa gtcatggatg acgagaggct gcggcagctc
     3001 accgaggcct ccaactacgt ctcggagctc atgaacctga cgcctgacac gcaccgtccc
     3061 tacgtgggac gcagcgcctt cgcccacaag ggcgggctgc acgtcgacgg catctccaaa
     3121 gaccccgcga cctacgaaca tgtcgcgccc gaggccgtag ggaacgtaag gcggacgcct
     3181 gttggggagc tctcgggaaa gaactccata aggaccaagg cgagggagct cgggctcgag
     3241 gccagggatg ctacccagat cctcggcgct ataaagcagc gcgagtacga gggctaccac
     3301 tacgaggccg ctgacgcctc gctcgcgcta ctgatagggc ggaccaccgg ggaggacagg
     3361 ccgctcttcg agttggagac gttcaggatc atctccgaga agcgggccgg tggagagacg
     3421 atgacggagg cgaccattaa gctctccgta ggcgggcagc gggtcatctc cactgcagag
     3481 ggcaacggtc ctgtaaacgc cctcgatcgg gcactgagga aagctatcgg gccgcactat
     3541 cccgagctga aagagatcca cctctccaac t
//
LOCUS       DATMJA010000397        10707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_171483, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000397 DATMJA010000000
VERSION     DATMJA010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171483"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1861
                     /locus_tag="VNT12_09470"
     CDS             203..1861
                     /locus_tag="VNT12_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HWI46387.1"
                     /translation="MEQRPEVPRIDSTVFWIAAVLSAVFVAWGIFFTNSLAAVFDAVL
                     WSFLVPNFGWMFILSTFGFLAFSLYLAFSRYGKVRLGGQDEQPEFSTVSWVAMMFSAG
                     MGIGLMFYGVAEPLTHMGAPPFGLAEPNTKGAAQVAMEYSYFHWAFHPWAIYAIMGLA
                     LAYFTFRKGMPNLVSTAFYPLLGDRVYGPIGKTIDILAIFATLFGSATSLGLGALQIN
                     QGLNAVFGIGGREAVGLAIVVIAVLTLAFILSAISGVHRGIQWIANTNMVLAVFLLLF
                     LFLVGPTVFILNTFTESIGGYLANIIPMSFRTASYGDSAFVSGWTIFYWAWWISWAPF
                     VGVFIARISRGRTIREFVLGVILAPSVVSFVWFSVLGGAAIDLQLTGAANLSELASAN
                     QPAALFATLQQFPLFLLTALIAIILVALFFVSGADAASVVMGMLSSKGNLHPKAWNVV
                     VWGVFTGAAAAVCLLAGAIQGSVDAALVALQSVAIASAAPFVLILIGLCFSILKALRA
                     EQLPTATPGRETPEPGRAASRPTGTAAPQQMSAENPDEQRGYPG"
     gene            1959..2411
                     /locus_tag="VNT12_09475"
     CDS             1959..2411
                     /locus_tag="VNT12_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWI46388.1"
                     /translation="MGRTRNPFRGLMDHMSEMTRMREYAEGGGGAEDQRRTHATAWVP
                     TADIFAKGEDLVIRCELAGVERDDVEVSLAGGVLTIDGERRGEPEDVSYYARERYYGR
                     FRRTMTLPEGIDGARVSADFENGLLEVTVKGGADHPEPERIQIGSSVG"
     gene            2698..3258
                     /gene="folE"
                     /locus_tag="VNT12_09480"
                     /pseudo
     CDS             2698..3258
                     /gene="folE"
                     /locus_tag="VNT12_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
     gene            3446..5893
                     /locus_tag="VNT12_09485"
     CDS             3446..5893
                     /locus_tag="VNT12_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI46389.1"
                     /translation="MESRAGVVIVGAGIVGCSVAEHLSRLGWRDVVVLDQGPLFEAGG
                     STSHAPGLMFQTNPSKMMTDLAKYSVGRYMELEVDGRPCGYQVGGIEVAETPERLADL
                     KRRHGLAASWGLKSHLISPDECAEKIPLLDTDKILGGFFVPSDGIAKGVRACEAMGRL
                     ASERGAKFYGETEVTGIEVKNGRVRAVETDKGRIETELVISCAGMWGPYIGRMVGMTV
                     PLMPMQHQFAWTTPLAELAGETREVMQPMLRHQDFSMYMRQREDYFGIGAYRHRPMPI
                     SADDIRRDYRGEGEFPSVLEFTPEDFEPTWQEARKMIPTLKNVEMARPMNGLFSFTPD
                     GGPLLGESKEAMGFWIAEAVWITHAIGVGKVMAEWLTDGVPSIDLAGADVHRFEPYAK
                     SPAYILARSSQSFREVYDIIHPQQPMEEPRPLRTSPYYSRQKDLGAYFLEASGWERPQ
                     WFEANQKLLDGQDDILGRGEWAGRYWSEIAAAEARATRERVALYDMTSLKRAEVTGPG
                     AMEFLQHLTTGQIDRPPGKVTYTLLLDEAAGVKSDITVARLEDERFQLGLNGPRDIEW
                     MERHLPSDGSVQVRDITPGTCCIGVWGPSARDVVQSLSDEDFSNEAFGFFNARYAYLR
                     EVPVTALRLSYVGELGWEIYTTSDMGLRLWDLLWDVGQPYGVVAGGRAAFNSMRIEKG
                     YRAWGTDMTTEHDPYEAGLGFAVKADKGDFIGREALAKRKEAGPRRRLVCTVLDDPNV
                     VVMGYEPVFVDGRAVGYVTSADFGYTLGQSIAYAWVPPEHAEVGSRLEIQYFDERYPA
                     TVTEEPLFDPAMKRMRA"
     gene            5991..7196
                     /locus_tag="VNT12_09490"
     CDS             5991..7196
                     /locus_tag="VNT12_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWI46390.1"
                     /translation="MAEYINPDPGILQYTRIFKSPYFYGSRRHGVQKYSVYNHHYHPR
                     YYRDPVEEYWQLLEGVTLWDVGGAERQVEITGPDAFEFTNMLTPRNLNKCAVGQCKYA
                     FITNAEGGIINDPVLLRLDENHFWLSLADSDVLLWAQGLAYNMGLNVEITEPDVGPMQ
                     VQGPKSREVMTHLFGEEILDVPYYYMVHRELDGMELVISRTGYSGELGYELYLKNASR
                     DGLKLWDRVLQAGEPHGISPIGPCHIRRIEGGILALGCDMWYDTNPYEVGYGYKWMVE
                     LEQEQDFVGREALQRISEEGVSRKLVGVEIGGSNCGSYNDGSMIDYFPVHKDGERVGK
                     VTSACYSPRLEKNIGYAMVPVEYSEIGIELEVERPGETVSATVADRVFFKPEQAEQNL
                     SATSSSKGA"
     gene            7263..8135
                     /locus_tag="VNT12_09495"
     CDS             7263..8135
                     /locus_tag="VNT12_09495"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWI46391.1"
                     /translation="MSTEQRSEQRQKSNRRLAEFLRHPGYEVIPLAGVEESVVEHVPR
                     NISLTVTASPGKGIPATVDLAERLAAHGFRVVPHLSARLIRDGSELAEILQRLREAGI
                     RDTFVVAGDVPEPAGEFEGAVDLLRAMAEIGHPFGKVGITGYPESHPIISDETTIQAM
                     YDKAPYADYIISQICFDPKTISDWARWVKRRGVDLPIRVGMPGVVNRQKLMRISATIG
                     LGESARFLLKQRNWLLKMFMPGGYSPNRLVLGLKPALADPESNIEGFHIYTFNDVEKT
                     EAWRREMLKRVEAA"
     gene            8171..9871
                     /locus_tag="VNT12_09500"
     CDS             8171..9871
                     /locus_tag="VNT12_09500"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWI46392.1"
                     /translation="MTIPKNLEVARKAKLAPLEDIAASIGIGKHLLEPYGEGVAKIKL
                     DAIEELEDRPRAKYVVVSAITPTPLGEGKTTTTVGLGQAFKQIGKTATVAVRQASMGP
                     VFGIKGGAAGGGYSQVVPMEMLNLHLTGDIHAVTAANNLLAAMVDNHIHHHDDFFHVD
                     RHSVTWRRVMDVNDRALRNIITGLGLRADGVPRETGFDITAASEVMAILALATSLQDL
                     RGRLGRIVIGNDTDGNPIMAEDLKAAGAMAVIMKEAIKPNLMQTLENTPALVHAGPFG
                     NIATGNSSIVADLIGIRGSDYLVTEAGFGADMGAERFFNIKCRNSGLKPDAAVLVATV
                     RALKVHSGRYEVKAGQPLPEEMLVENPGDVRAGADNLRKQIENIRLHGVSPVVAVNSF
                     PTDYDSEHDAIREVAEEMGARVAICNHFSEGGKGAVELAEAVAEAAEEPTEFRFLYPE
                     EASLKEKIEAIATKVYGADGVEYDLTASRQLDSYEKNGFGNLPICMAKTHLSISSDPS
                     LRGAPKGWTLPVRQVRASVGAGFIYPICGDMRTMPGLSIHPAAHEIDIDEDGQIVGLF
                     "
     gene            9884..10483
                     /locus_tag="VNT12_09505"
     CDS             9884..10483
                     /locus_tag="VNT12_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HWI46393.1"
                     /translation="MPTPENIAPDYLDLPLGEFLDRVASDSPAPGGGSAAAIAVALAA
                     GLAGMAARLSTGQLSDAPELADQADASRHGVAPLARADAESYGRVLDAYRESDPETRM
                     ARVRDALSGAADVPLAVAEAGSEVVAIASRLAEEGNPNLEGDALAAVLLAGAGVRAAV
                     ALVRINLSTANVDDDRLKRANSLADTTAAAVGRATEGDG"
     gene            10542..>10707
                     /locus_tag="VNT12_09510"
     CDS             10542..>10707
                     /locus_tag="VNT12_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HWI46394.1"
                     /translation="MLERRTPFYDFHRRSARDLVKGGGNFMFPTSYASSLEEHLNVRR
                     NVGMQDLSTMG"
BASE COUNT         2072 a         3245 c         3563 g         1827 t
ORIGIN      
        1 aaggcgaacc gtagaagagc cgcagatcct gaaggccccg taagagcgtc cccatcgaga
       61 gaaggccaac gccgccgacg gtccgagtga gcttgaaaca cgttgcacta tccggtacag
      121 tgttggcgtt ttcagaccag ggtgttaggt tagtgtatgc ttgataggtg gccgtccgaa
      181 ggggagaaag ggaaggagac atgtggagca aagacctgag gttccgagga tcgatagcac
      241 cgtattttgg attgcagcag ttctctcggc ggtgttcgta gcatggggga tattcttcac
      301 aaatagcctg gccgccgtct tcgatgctgt cctgtggagc ttcctggtgc cgaacttcgg
      361 ctggatgttc atattgtcga ccttcggctt tttggccttc tcgctgtacc tggccttcag
      421 ccgctacggg aaagttcgac taggcggcca ggacgagcag ccggagttca gcaccgtctc
      481 gtgggtggcg atgatgttca gcgcggggat gggaataggc ctcatgttct acggcgtggc
      541 cgagcccctt acccacatgg gtgccccccc gttcgggttg gctgagccca acaccaaggg
      601 ggccgcccag gtcgcgatgg agtactccta cttccactgg gctttccacc cgtgggcgat
      661 ctacgcgatc atggggctgg cgctcgccta ctttactttc cgcaagggga tgcccaacct
      721 cgtcagcacg gcgttctacc cgctgctggg cgacagggtc tacgggccca taggcaagac
      781 catcgacatc ctggcgatct tcgccaccct gttcggctcg gccacctcgc tgggcctggg
      841 cgccctgcag atcaaccagg gcctcaacgc ggtgttcggg ataggaggac gggaggccgt
      901 cgggttggcc atagtggtga tcgccgtctt gaccctggcg ttcatcctgt cggcgatcag
      961 cggggtacac cgcggcatac agtggatcgc caacaccaac atggtgctgg ccgtgttcct
     1021 gctgctcttc ctgttcctcg tgggaccgac ggtgtttatc ctcaacacct ttacggagtc
     1081 gatcggcggc tacctggcca acatcatccc gatgagcttc aggacggcct cctacggcga
     1141 ctcggccttt gtttcgggct ggaccatctt ctactgggcg tggtggatca gctgggcgcc
     1201 gttcgtaggc gtctttatag ccaggatctc ccgcggccgt accatccgtg agttcgtctt
     1261 gggcgtgatc ctggcgccca gcgtcgtcag cttcgtatgg ttctccgtcc tcggcggggc
     1321 ggccattgac ctgcagctca cgggcgcggc gaacctctcg gagctcgcct cggccaacca
     1381 gccggcggcg ctgttcgcga ccctgcaaca gttcccgctg ttcttgctta cggccctgat
     1441 cgcgatcatc ctggtcgcgc tgttcttcgt cagcggggcc gacgcggcct cggtggttat
     1501 gggcatgctt tcttccaagg gcaacctgca cccgaaggcg tggaacgtcg tcgtgtgggg
     1561 cgtctttacc ggtgcggctg cggcagtctg cctgctggcc ggggccatcc agggctcggt
     1621 tgacgcggcc cttgtagctc ttcagtcggt ggccatcgcg tcggcggcac ctttcgtgct
     1681 tatcctgatc gggctgtgct tctccatttt gaaagcactg cgcgccgagc agttgcccac
     1741 cgcgactccc ggacgcgaga ctcccgagcc ggggagggcg gcatcccgcc cgaccggcac
     1801 cgcggcaccg cagcagatgt cagcggagaa ccccgacgag cagcgcggct accccggcta
     1861 gaagagccag gattcagacc gacctcgtaa cacaacacgg gcgggtggct tcggccatcc
     1921 gcccgtcgta ctaggacgag accagagaag gaggaaatat gggcaggacg cgcaacccat
     1981 ttcgggggct catggatcac atgagcgaga tgaccaggat gcgggagtac gccgaggggg
     2041 gcggcggggc tgaagaccag cgcaggaccc acgcgaccgc ctgggtcccg acggccgata
     2101 tattcgccaa gggtgaagac ctggtgatac gctgcgagct ggccggggtg gagagggacg
     2161 acgtggaggt ctccctcgcc ggcggcgtcc tgaccatcga cggggagcgc aggggagaac
     2221 cggaggacgt gagctactac gcccgagagc gctactacgg gcgcttccgg cggaccatga
     2281 ccttgccgga aggcatagac ggcgccaggg tcagcgccga cttcgaaaac ggtttgctgg
     2341 aggtcaccgt gaagggcggg gccgaccatc ccgagccaga gcgtatccaa ataggaagca
     2401 gcgtcggtta gccaattcgg gtaagcagag gacgccactg agcccggctc ccacacgcag
     2461 ccaggctcat tggcgtccgg acggtcgcgc tttcgaggcc gcgggtgccc gccatgtcct
     2521 tctcctccgt cattccgcag aaggcgtatg ccccgcgcgt tggcacccct attcaccggg
     2581 agcaggtatg aaatgccgac ggctcgctgt acctttatcc gaaacaagat gcgtcatgcg
     2641 gtacgggttc tagtatgatg atggaggaga catcatacaa gaaccgggga acgtcgcatg
     2701 gataagctcc gcatcgagcg ggccgtgaaa gagatattgc tcgccatagg ggaggaccct
     2761 gaccgcgagg gcttgcgggg tacgccccgc agggttgcgg aggcctacga gatgctcttt
     2821 gccgggttgg aagaggatcc cgtccggcac ctggaggtgg gcttctagga aaaccaccgc
     2881 gagatggtcc ttgtcaggga cataaccctg cattcgctct gtgaacatca ccttctgccg
     2941 atggttggca aggcccacat tggctacata ccggacggga aggtggtcgg gttgtccaag
     3001 ttggcccggt tggtggaggg ttacgcccgc cgtccccagc ttcaggagcg cctgaccacc
     3061 cagatcgccg acgcgttgca caacgagttg ggagctctcg gcgccatagt agtgatcgaa
     3121 gccaaccacc tgtgcatgac gatcaggggc gtccagaaac cggggagcat ggccgtcacc
     3181 agcgctgtgc gcggaactta cgccaaagac caacgcaccc gccaggaggc cataagcctc
     3241 ataacagcca accgctagcc gacacctccc cacagggccg gaaccacccg accgaccacc
     3301 ggtattccag ccccgacgac ttcttgactc tcgccctgtc gtgtgttagt tgtgccgtat
     3361 ataggaaagg acttcgccat gcgcaacaaa cctcgaacat gtccgtgtgg cttccgtagt
     3421 acggcggggg cttcgggggt ggtgtatgga gagtagagct ggggttgtga tcgtgggtgc
     3481 cggcatcgtc ggctgcagcg tggccgagca tttgagtcgg ctcggctggc gggacgtggt
     3541 ggtcctggat cagggaccgc tcttcgaggc tggcgggtcc acctcccacg ctccaggtct
     3601 catgttccag accaacccat ccaagatgat gactgacctg gcgaagtaca gtgtcggccg
     3661 ctacatggaa cttgaggtgg acggtcggcc atgtggctac caggtcggcg gcatagaggt
     3721 tgcggagaca cccgagcggc tggctgatct caagcgcagg cacggcctcg ccgcctcgtg
     3781 gggtttgaag tcgcacctta tctcccccga tgagtgcgcg gagaagatac cgctgcttga
     3841 cacggacaag atcctgggtg gcttcttcgt accttccgac ggcatagcca agggcgtcag
     3901 agcgtgcgag gcgatgggac ggctggcctc cgaacgcggc gcgaagttct acggcgagac
     3961 cgaggtaacc ggcatcgagg tgaagaacgg cagggtcagg gccgtggaga cggacaaggg
     4021 acgcatcgag acggagttgg taattagctg cgccgggatg tggggacctt acatcgggcg
     4081 catggttggc atgacggtgc cgctcatgcc gatgcagcat cagttcgcgt ggaccacgcc
     4141 gctcgccgag cttgcgggtg agacgcgaga ggtcatgcag cccatgctgc gccaccagga
     4201 cttctcgatg tacatgcgcc agagggaaga ttacttcggc atcggagcct acaggcaccg
     4261 tcccatgccc atctctgccg acgacatcag gcgcgactac aggggcgagg gcgagttccc
     4321 gtcggtgctt gagttcacgc cggaggactt cgagccgacc tggcaggagg cgcggaagat
     4381 gattcccacg ctgaaaaacg tcgagatggc ccgtccgatg aacgggctgt tctccttcac
     4441 gccggatggt gggccgttgc tcggcgagtc gaaggaggca atggggttct ggatcgcgga
     4501 ggcggtctgg atcacccacg ccatcggcgt gggcaaagtg atggccgagt ggctaaccga
     4561 cggcgtcccg agcatagacc ttgccggggc cgacgttcac cgcttcgagc catacgcaaa
     4621 gagcccggcc tacattctgg cccgctcctc gcaatccttc cgcgaggtct acgacatcat
     4681 ccacccccag cagccgatgg aggagccacg cccgctgcgg accagcccgt actattcacg
     4741 gcagaaggat ctgggcgcgt acttcctcga agcatcaggg tgggaacgtc cgcagtggtt
     4801 cgaggcgaac cagaagttgc tcgacggcca ggacgatatt ctcggacgtg gtgagtgggc
     4861 cgggcggtac tggtccgaga tagccgccgc cgaggcccgc gccacccgcg agcgtgtcgc
     4921 cctctacgac atgacgtcgc tcaagcgcgc cgaggtcaca gggccgggcg cgatggaatt
     4981 cctgcaacat ctaaccacag gccagatcga ccgtccgccg ggcaaggtga cgtatacgct
     5041 cctgctggac gaagccgctg gcgtgaaaag tgacataacc gtcgcccgcc tggaagacga
     5101 acgcttccag cttggactca acggccctcg ggacatcgag tggatggaac gccatctgcc
     5161 atccgacggc tccgtgcagg tgcgggacat cacgccggga acctgctgca tcggagtctg
     5221 gggaccttcc gcccgcgacg tggtgcagag cttatccgat gaggatttct caaacgaagc
     5281 cttcgggttc ttcaacgcca ggtacgccta tctgcgtgag gttccggtga cggcccttcg
     5341 gctttcgtac gtcggggaac tcgggtggga gatctacacg acatcggaca tgggactgcg
     5401 gctgtgggat ctgctatggg acgtcgggca accgtacggc gtcgtggcgg gtggccgtgc
     5461 cgccttcaac tccatgcgca tcgagaaggg ctaccgtgcc tggggcacgg acatgacaac
     5521 cgagcacgac ccgtatgagg ccggcctcgg cttcgcggtg aaggcggaca aaggagactt
     5581 catcgggcgc gaggcgctgg cgaaacgcaa ggaggctgga ccgcgccgca ggctggtctg
     5641 cacggtgctc gacgatccta atgtggtcgt gatgggatac gagccggtat tcgtggatgg
     5701 ccgggcggtc gggtacgtaa cgagcgcgga tttcgggtac acgctcgggc agagcatcgc
     5761 gtacgcgtgg gttccgcctg agcacgccga ggtcgggagc aggctggaga tccagtactt
     5821 cgatgagcgc tatccggcca cggtaaccga ggaaccgctc ttcgatcccg cgatgaagag
     5881 gatgcgagcg taggtgatgc gaagtacgac gtaaggaagg accagggttt tcccagagga
     5941 gtatctgtcc gatcgaagat cgagagacat cgagagagga gaagcgttct atggccgagt
     6001 acattaatcc cgaccccggc atactgcagt acacgaggat attcaagtcc ccgtacttct
     6061 acggttcgcg ccggcatggg gtccagaagt acagcgtcta caaccaccac taccacccac
     6121 gttactacag ggaccctgtc gaggagtact ggcagctcct agaaggcgtt accctgtggg
     6181 acgtcggggg ggccgagcgg caggtcgaga tcacgggtcc cgacgccttc gagttcacga
     6241 acatgctcac cccacgtaac ctgaacaagt gcgccgtggg ccagtgcaag tacgccttta
     6301 tcaccaacgc cgagggcggc atcatcaacg atcctgtgct cttgcgtctc gacgagaacc
     6361 atttctggct ctccctggcc gacagcgacg tgttgctctg ggcgcagggg ctcgcctaca
     6421 acatggggct gaacgtggag atcacggagc ccgacgtcgg ccccatgcag gtgcagggac
     6481 cgaagtccag ggaggttatg acccacctct tcggcgaaga gatactcgat gtaccctact
     6541 actacatggt gcacagggaa ctggacggga tggagctcgt gatctcccgc accggatact
     6601 ccggggagct ggggtacgaa ctctacctca agaacgcgag ccgcgacgga ctcaagctct
     6661 gggaccgcgt gctgcaggcc ggcgagccac acgggatctc ccccatcggg ccctgccaca
     6721 tccgcaggat cgagggcgga atcctggccc tgggttgcga catgtggtac gacaccaacc
     6781 cctacgaggt gggctacggc tacaagtgga tggtagagct ggagcaggag caggacttcg
     6841 tgggaaggga agcactgcag aggatcagcg aggaaggcgt cagccgtaag ctcgtgggcg
     6901 tcgagatcgg cggtagtaac tgcgggtcgt acaacgacgg ctccatgatc gactacttcc
     6961 cggtccacaa agacggcgag agggtcggca aggtcacctc ggcctgctac tcgccgcgcc
     7021 tggagaagaa catcggatac gccatggtcc ccgtcgagta cagcgagata gggatcgagc
     7081 tcgaggtcga gcggcccggg gagacggtct ccgccaccgt ggccgaccgc gtcttcttca
     7141 aaccggagca ggccgagcaa aacctgagcg ccaccagctc gtccaaggga gcctgaggga
     7201 ggctacgctg gccgggtaca ctaaaagccc ggccacccga ccctcggcct gtggagaaag
     7261 acgtgtcgac ggagcaaaga agcgagcaga gacagaagtc gaacaggagg ctggccgagt
     7321 ttctgcggca tcccggctac gaggtgatac ccctcgcagg ggtagaagag tcggtggtcg
     7381 aacatgtccc taggaacata agcctcacgg ttacggcctc gccgggcaag ggtataccgg
     7441 ccacggtgga cctcgccgag cgattggcgg cacacggctt ccgcgtggtg ccacacctct
     7501 cggcccggct catacgggac gggtcggagc tcgccgagat cctgcagagg ttacgggagg
     7561 cgggaatacg ggacaccttc gtcgtggctg gcgacgttcc cgagcccgcc ggggagttcg
     7621 agggcgcggt cgacctgctc cgggcgatgg cggagatcgg gcacccgttc gggaaggtcg
     7681 gcatcacggg gtatcccgag agccatccga tcatcagcga cgagaccacg atccaggcga
     7741 tgtacgacaa ggccccgtac gccgactaca tcatcagcca gatctgcttc gatccgaaga
     7801 ccatctcgga ctgggcccgg tgggtgaagc ggcggggtgt ggatctcccg atccgggtcg
     7861 gaatgcccgg cgtcgtgaac cgccagaagc tgatgcggat ctccgcgacc atcggcctcg
     7921 gcgagtcagc gcgcttcctc ctgaagcagc gcaactggct gctcaagatg ttcatgccgg
     7981 gcggatacag cccgaaccgc ctggtactcg gcctgaagcc cgccctggcc gaccccgaga
     8041 gcaacatcga gggcttccac atctacacct tcaacgacgt ggagaagacc gaggcgtggc
     8101 ggcgtgagat gctgaagcgc gttgaagcgg cgtgatgacc ctacacagtt agaaccagga
     8161 gtttgccagt atgacgatac cgaagaacct cgaggttgcc cgcaaagcga agctcgcgcc
     8221 gctggaggat atcgccgcct ccataggcat cgggaagcac ctgctggagc cctatggcga
     8281 gggcgtagcc aagatcaagc tggatgcgat agaggaactg gaggatcgtc cgcgggcgaa
     8341 gtacgtggtc gtatcggcca taacgccgac tcccctgggc gaggggaaga cgacgaccac
     8401 cgtggggctc ggacaggctt tcaagcagat cggcaagacg gcgacggtag cggtgcggca
     8461 agcctcgatg gggccggtgt tcgggatcaa gggcggcgct gcgggcggcg gctacagcca
     8521 ggtggtgccg atggagatgc tcaatttgca cctgaccggc gacatacacg cggtcaccgc
     8581 tgccaacaac ttgctcgccg ccatggtgga taaccacatc caccaccacg acgacttctt
     8641 ccacgtggac cggcactccg tcacgtggcg acgggtgatg gacgtaaacg accgggcctt
     8701 gaggaacatc atcaccggcc tgggactcag ggcggacggg gtcccccgcg agacgggctt
     8761 cgacataacg gcggcctccg aggtgatggc aatcctggcg ctcgcgacct cgctgcaaga
     8821 cctcagaggg aggctggggc gcatcgtgat cggcaacgac accgacggga accccattat
     8881 ggccgaagac ctcaaagcag cgggcgccat ggccgtgatc atgaaggagg ccataaagcc
     8941 gaacctgatg cagacgctgg agaatacccc ggctctggta cacgccggtc cctttggcaa
     9001 catcgccacc ggcaactcct cgatagtggc agacctgatc ggcattcgcg gtagcgatta
     9061 cctcgtaacc gaggcgggct tcggcgctga catgggggcc gagcggttct tcaacatcaa
     9121 gtgtcgcaac tcgggattga agcccgacgc ggcggtgctc gtggcgacgg tgcgggcgct
     9181 gaaggtccac tcgggccgtt acgaggtgaa ggccggacag ccgctgcccg aggagatgct
     9241 ggtggagaac cccggcgacg ttcgcgccgg ggctgacaac ctgcggaagc agatcgagaa
     9301 catcaggttg cacggcgtct cgccggtggt ggcggtcaac tctttcccca ccgactacga
     9361 ctcggagcac gatgccatcc gggaggtggc cgaggagatg ggcgcccgcg ttgcgatctg
     9421 caaccacttc tcggagggcg gcaagggggc cgtggaactg gccgaggcgg tcgccgaagc
     9481 cgccgaggag ccgaccgagt tccgcttcct ctaccccgaa gaggcgtctc tgaaggagaa
     9541 gatcgaggcc atcgccacca aggtgtacgg ggcggacggc gtcgagtacg acctgaccgc
     9601 gagccgccag ctcgactcct acgagaagaa cggcttcggc aacctgccca tctgcatggc
     9661 gaagacacac ctctccatct cctcggatcc ctccctgagg ggtgcgccga agggctggac
     9721 cctgccggtg cgccaggttc gcgcttcggt gggggccggc ttcatctacc cgatctgcgg
     9781 cgacatgcgc accatgccag gcctcagcat ccaccccgcc gcccacgaaa tagacataga
     9841 cgaggacggt cagatcgtcg gcctgttctg agaggacatc ctggtgccca caccggaaaa
     9901 catcgctccg gactacctgg acctcccgct aggagaattc ctcgaccgcg tggcctccga
     9961 cagtcccgct ccaggcggag gctccgcggc cgccatagcc gtcgccctcg ccgcagggtt
    10021 ggcaggcatg gccgcccgcc tgtccacagg ccaactgtcc gacgccccgg agctggcaga
    10081 ccaggcggat gcctccaggc acggggtcgc acccctcgcc agggccgacg cggagtccta
    10141 cgggcgagtc ctcgacgctt accgcgagtc cgaccccgag acgcgtatgg cgcgtgtcag
    10201 ggacgcgctc tcgggggccg ccgacgtgcc gctagccgtg gccgaggccg ggagcgaggt
    10261 cgtcgccatc gcgtcccggc tcgctgaaga gggcaacccc aacctcgaag gagatgcgct
    10321 ggcggcggtt ctgctggcgg gcgcgggggt acgcgcggcg gtggcgttgg tcaggatcaa
    10381 cctgtccaca gcgaacgtgg acgatgatag gctcaaaagg gcgaacagtc tcgccgatac
    10441 caccgcagcg gctgtgggcc gggccacgga aggggacgga tgaacgcgat agaagcttgc
    10501 agtataatat acaaacgata cgtttgaggg aagggtattc aatgttggag agaaggacgc
    10561 cgttctacga cttccaccgc cgttcggcca gggatctcgt caaaggtgga ggaaacttca
    10621 tgtttcccac ctcctacgct tcgtctctgg aggagcacct gaacgtgcgc aggaacgtgg
    10681 ggatgcagga tctctcgacg atgggcg
//
LOCUS       DATMJA010000398         5322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_704000, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000398 DATMJA010000000
VERSION     DATMJA010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704000"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VNT12_09515"
     CDS             <1..300
                     /locus_tag="VNT12_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI46395.1"
                     /translation="SGMLAEITNMQLMCYRLAQLQEQGKMTGPMASLAKMNNARKAKQ
                     VCADARDIMGGNGILLEYHVARHLSDMEIVYTYEGTDTIQSLIVGRDVTGISAFV"
     gene            507..1523
                     /locus_tag="VNT12_09520"
     CDS             507..1523
                     /locus_tag="VNT12_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWI46396.1"
                     /translation="MKAVRLHKLEGPDELVYEDVPEPEPGPGETVVRLRAAALNRRDL
                     FATQGQYPGVTPDILPRIPGSDGSGEVVARWDGAEGPEEGTEVVINPALYWGDDPRVP
                     GKEYRILGIPDDGTFAQFIKVPADHVFPKPSHLSYEEAAALPLAALTAYRALVTRGGV
                     AEGETVLVPGVGGGVATFLVQIASALGARVFVTSGSDEKIEDSKELGAEGGVNYNSEN
                     WSRELKSMSGGVDLSADHVGGENFNSLVSLAKPGGRIVVIGATAGPTSKAMTIRIALK
                     QLDVFGTAMGTAEEFGAMLDLYAEHGLHPKVNETFALEDTASALHLMEEGSGIGKIVL
                     DVPA"
     gene            complement(1627..1827)
                     /locus_tag="VNT12_09525"
     CDS             complement(1627..1827)
                     /locus_tag="VNT12_09525"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HWI46397.1"
                     /translation="MDFDPDDTTQYLEGVDYPASKEDLASTAEGNGAPEELVERLRTL
                     GRPTFSGPGEVLAELESSPTSG"
     gene            complement(1957..3348)
                     /locus_tag="VNT12_09530"
     CDS             complement(1957..3348)
                     /locus_tag="VNT12_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWI46398.1"
                     /translation="MKSMKLGVWVLVGMGLAMLLSVGVLGSEGSVSAQDSTDPILVGA
                     GDIAGCGTRTVPRIGDEATAKLLDTIVAAAPSTTAVFTTGDNAYESGTAFEFANCYDP
                     TWGKFKARTMPSPGNHEYYDSSEVARPYFDYFSATNGFEQPAAPVPNDPVNDNDHGLK
                     IGEGYYSYDRGSWHMISLNSNCSFVAGGGCAAGSDQVRWLKADLAAHSNACTLAYWHH
                     PLFSSGSTSGGNSKMKPFWEALYAAKADVVLNGHVHNFEVFRPQTPSGVANPAGIREF
                     VVGTGGYSLNAFKTTVANSQERYRATNGVLKLTLHSTSYDWRFVAEDGTTPPGLSGSD
                     SCDPDGTTLPDTTPPTVESTIPSAKEIEVATTIDVTATFSEKMMETSINGTTFKLVKL
                     NADGTTTKVTADVSYDVATKKAILNPSGDLSPGGTYKATVTSGAQDLQNNALDQKPNI
                     AGNQNKSWKFTVQ"
     gene            3720..4331
                     /locus_tag="VNT12_09535"
     CDS             3720..4331
                     /locus_tag="VNT12_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HWI46399.1"
                     /translation="MSGGFISRGFRGRRRRAEGDVRIPPGQYLVEGFPVLSAGPTPRT
                     PLEEWDFTIEGEVDGPRTWSWEEFRALPSEEITRDIHCVTKWSKLGTTWEGVSVDTLL
                     EEVDTAAEYMVAFCDGGYTTNLPLEEVTGGKAWVAHTYEGKPISPEHGGPARLLVPHL
                     YFWKSAKWVRGLRLVAVDEPGFWESLGYHNHGDPWREQRYWGD"
     gene            4291..5079
                     /locus_tag="VNT12_09540"
     CDS             4291..5079
                     /locus_tag="VNT12_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HWI46400.1"
                     /translation="MATRGASNATGVIRVAGAPRKLAWRIGEIVETSPDTPRTKSLFL
                     EIPGWDGHRAGQHVDVRLTAEDGYQAQRSYSIASAPEDGRVELLVERLDDGEVSPYLT
                     EELRAGDGLELRGPIGGWFAWEAREGGPLLLVAGGSGIAPLMAMIRHRQAAGNDTPVR
                     LLFSSRSYEDVIFREELERLAAEDETLEIAHTLTRSAPPGWKGYDRRIDEEMLGEVAF
                     PPGERPLAFVCGPTPLVEAVATTLVGLGHEPARVKTERFGPTGG"
     gene            5082..>5322
                     /locus_tag="VNT12_09545"
     CDS             5082..>5322
                     /locus_tag="VNT12_09545"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HWI46401.1"
                     /translation="MDEHRLDGNAAAGVLAEIFTFEITTARAACTRCGATGEIGAQMA
                     YVTEIGTVVRCAACDNALIRVVRQDDGPGRYWLDLK"
BASE COUNT          984 a         1608 c         1721 g         1009 t
ORIGIN      
        1 tcgggcatgc tagccgagat caccaacatg cagctcatgt gctaccgcct tgcgcaactc
       61 caggagcagg gcaagatgac cggaccgatg gcctccctgg ccaagatgaa caacgcgagg
      121 aaggcaaagc aggtctgcgc cgacgccagg gacatcatgg gcggcaacgg catcttgctc
      181 gagtaccacg tagcgaggca cctctccgac atggagatcg tctacacgta cgaaggcacc
      241 gacacgatcc agtcgctgat agtgggtcgt gacgtaacag gcatttcggc ctttgtgtag
      301 aaaagcaagt cagcacgctc cggccttcgg cctccgcttg tcagcatttc agctaaagtc
      361 ctttagggat ggaacgtcac cccggcaaca gtgctggcgt agcgagcgaa cccctcgcgt
      421 gaaagcgcgg ggcccgcatt cgtactaagc tgactacctg acaagccgcc tgcaaggcgg
      481 catgctgagc gaccgaaggg agcgacatga aagctgttcg actgcacaaa ctcgagggtc
      541 ctgacgagct cgtttatgag gacgtgcctg agccggagcc cggacctggt gagaccgtgg
      601 tccggctccg ggcggcggcg ctcaaccgcc gggatctttt cgccacccag ggccaatatc
      661 ctggcgtcac ccctgacatc ctgcccagga ttccgggctc cgatggttcc ggagaggttg
      721 tcgcccggtg ggacggtgcc gagggaccgg aagaagggac tgaggtagtc ataaacccgg
      781 cgttgtactg gggagatgat ccgcgggtgc ctggcaagga gtaccgtatt ctcggtattc
      841 cggacgacgg gactttcgcc cagttcatca aggtaccggc ggatcacgtc ttccccaagc
      901 cctcgcatct ttcgtacgag gaagccgctg cgctgccgct ggccgccctg accgcctaca
      961 gggcgcttgt aacgcgcggc ggggtcgctg agggcgagac cgtcctcgtg ccaggcgtcg
     1021 gcggcggggt ggcgacattt ctggtccaga tcgccagcgc cctcggcgcc cgcgtcttcg
     1081 tcacctcggg gagcgatgag aagattgagg attcaaagga actcggcgcc gaggggggcg
     1141 taaactacaa ttcggaaaac tggtccaggg agctcaaaag catgtccggg ggcgtggatc
     1201 tctcggcaga ccacgtcggc ggtgaaaact tcaactcgct cgtctcgctc gccaaaccgg
     1261 gcgggcggat cgtcgtcatc ggcgcgacgg ccggtccgac gtcgaaagct atgaccatcc
     1321 gcattgctct aaagcagctc gacgtgttcg ggactgcgat gggcaccgcc gaagagttcg
     1381 gcgcgatgct cgacctctat gccgagcacg gcctccatcc aaaggtaaac gagaccttcg
     1441 cgctcgaaga taccgcctcg gccctccacc taatggaaga aggtagcgga ataggcaaga
     1501 tagtcctgga tgtacccgca tagtcctcaa ggacgagcgg ggcgcggtgc tcggcggggc
     1561 tcttggtcac atctggggag catggctcga cctcgatacc ctgtgtgttg cggagccgtg
     1621 acaggatcat cctgaggttg gcgaagactc tagctctgcc aaaacctcac ccggacccga
     1681 gaaagtggga cggcccaagg tacggagcct ctccaccagc tcctctggcg ccccgttgcc
     1741 ctcggcggtt gaggccagat cctccttgct cgctggataa tcgacgcctt ccaggtactg
     1801 cgtcgtgtcg tcagggtcga agtccacgat agcctcctct gggttcgttg acaccaccct
     1861 atatgcccct gaaaccgagg ttcgtaacgc tacgagggaa gggctgcgat gctctaagca
     1921 gccccggccc tccgcccgtg cggcgagcct acgctcctac tgcaccgtga acttccagct
     1981 cttgttctgg ttgcccgcta tgttcggttt ctggtcgagg gcgttgttct gcaagtcctg
     2041 cgccccgctc gtcaccgtgg ccttgtaagt cccccctggg ctgaggtcgc cagaggggtt
     2101 gaggattgcc ttcttggttg cgacgtcgta gctgacgtcg gcggtaacct tggtagtggt
     2161 gccgtccgcg ttgagcttga ccagcttgaa ggtcgtgccg tttatggagg tttccatcat
     2221 cttctcagag aaggtcgccg tcacgtcgat tgtggtggca acttcgattt ccttggcgct
     2281 agggatggtg ctttcgaccg tgggtggtgt ggtgtcgggg agcgtcgtac cgtcagggtc
     2341 gcagctatcg ctgccgctga ggccaggggg tgtcgtgccg tcctcggcga cgaacctcca
     2401 atcgtagctc gtcgagtgca gcgttagctt caagactcca ttggttgctc tatatcgctc
     2461 ctggctgttg gccacagtgg tcttgaaggc gttcaaagag taaccgccgg tacccaccac
     2521 gaactcgcgg atgcccgcag ggttcgccac cccactgggc gtctgtgggc ggaagacttc
     2581 gaagttgtgg acgtggccgt ttaagaccac gtccgctttg gccgcataga gggcttccca
     2641 aaacggcttc atcttggaat tgcctccgct cgtcgatccc gagctgaaga gtggatggtg
     2701 ccagtaggca agcgtgcacg cgttcgagtg ggcggcaaga tcggccttca gccaccgcac
     2761 ctgatcggat ccggcggcgc aacctcctcc cgccacaaac gaacagttag agttcagcga
     2821 gatcatgtgc cagcttcccc gatcgtagct gtagtagccc tcgccaatct ttagtccgtg
     2881 atcattgtca ttgaccggat cgttcggcac cggcgccgcg ggctgctcga agccattggt
     2941 cgcgctgaag tagtcgaagt agggccgcgc tacctcggag gaatcgtagt actcgtggtt
     3001 gcctgggctg ggcatggttc gcgccttgaa cttgccccag gtggggtcgt agcagtttgc
     3061 gaactcgaag gcggtgcctg actcgtaggc gttgtctccg gtcgtgaaca cagctgttgt
     3121 cgagggtgca gccgcgacga tggtgtcgag gagcttagcg gtagcttcgt cacctatgcg
     3181 gggaactgtg cgggtgccgc agccagctat gtcccctgcc cctacgagga tcggatctgt
     3241 actatcctgg gcgcttacgc tcccctcgga gcccaaaacc cccaccgaga ggagcattgc
     3301 cagtcccatg cccaccagaa cccagacccc gagcttcata ctcttcatgg acacccctcc
     3361 tatgctacac ccaacgccaa cgtaggaaag atagcgatac aggcatccaa ttgcaacgca
     3421 cagctgaaga gttgctcgag acaattctgg gacggcgtcc tccgtggtag tatggctatg
     3481 aacatcgcag cgctagccac cactcggatt gcaagacgac tatcagatat cagggcaccc
     3541 atcttctgta cgcatccagc cattgagtgt ttgccggcgt attgcgggta cgcgaactgc
     3601 ttggaacgtg cgggacctcc ggcacggaga cccgccaggt acgcgctttt gactctgcct
     3661 tcggcggcgt gcaagaatgg aggtattctc ggcggagtac gacggcagga ggtacattcg
     3721 tgagtggagg attcatctct cgcggctttc gaggccggcg caggcgggct gagggcgatg
     3781 tgcgaatccc gcccggccag tacctggtgg agggcttccc cgtcctctcc gcgggcccga
     3841 cgccacgcac acctctggag gagtgggact ttacgatcga gggcgaggtc gacgggccga
     3901 ggacttggtc gtgggaggag ttccgggctt tgccttcgga agagatcacc agggacatcc
     3961 actgcgtcac caagtggtcg aagctgggga cgacctggga aggtgtctcc gtggacacat
     4021 tgctggaaga ggtggatact gcagcggagt acatggtcgc attctgtgat gggggctaca
     4081 cgacgaatct tcctctagaa gaggtcacgg gaggcaaggc ctgggtcgcc cacacctacg
     4141 aaggaaaacc gatctcgccg gagcatggcg gtccggcccg cctgctcgtc ccgcacctct
     4201 acttctggaa gagcgccaag tgggtaaggg gcctcaggct cgtggccgtg gacgagcctg
     4261 gtttctggga gtccctcggc taccacaatc atggcgaccc gtggcgcgag caacgctact
     4321 ggggtgatta gggtggccgg cgccccgcgc aaactcgcct ggcgtatcgg cgagatcgtc
     4381 gagaccagcc cagacacgcc gcgaacgaag agcctgttcc tggagatacc gggctgggac
     4441 ggccacagag ccggccagca cgtcgatgtc aggctcacgg cggaggacgg ctaccaggcc
     4501 cagcgcagct actctatagc ctcggcgccg gaagacggca gggtcgagct cctcgtcgag
     4561 cgtctggacg atggtgaggt ctctccctac ctgacggagg agctcagggc cggggacggg
     4621 ttggagttgc ggggtccgat cgggggctgg ttcgcctggg aggcgaggga gggcgggcca
     4681 ttgctcctcg ttgccggagg atcgggcata gcgccgctca tggcgatgat ccgtcaccgc
     4741 caggcagcgg gcaacgacac ccctgtccgc ctgctcttct cctcgcgctc ttacgaggat
     4801 gtcatcttcc gcgaagaact ggagcgtctt gcggccgagg acgagacgct ggagattgcc
     4861 cacacgctca ctcgctcggc gccgcccggc tggaagggtt acgaccgcag aatcgatgaa
     4921 gagatgctcg gggaagtcgc cttcccccct ggcgagaggc ctctggcctt cgtatgcggt
     4981 cctaccccgc tggtcgaggc cgtcgcgacg acgctcgtcg ggctaggcca cgagccggcg
     5041 cgcgtcaaga ccgaacgctt cggaccgact ggaggataac gatggacgaa cacaggctcg
     5101 acggcaacgc cgcggcaggg gtactggccg agatcttcac gttcgagatc accacggcca
     5161 gggccgcctg cacccgctgc ggcgccaccg gtgagatcgg cgcccagatg gcctacgtga
     5221 ccgagatagg tacggtggtt cgctgcgccg cgtgtgacaa cgccctgatc cgggtcgtcc
     5281 gccaggacga cggacctgga cgttactggt tggatctcaa gg
//
LOCUS       DATMJA010000399         4470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_230780, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000399 DATMJA010000000
VERSION     DATMJA010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230780"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..1376
                     /locus_tag="VNT12_09550"
     CDS             390..1376
                     /locus_tag="VNT12_09550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWI46402.1"
                     /translation="MIALGLVGDVMLGRGVDEALRGMRPDEPWGDALSVLDAADLRVI
                     NLECAITTHENPWSRTPKTFHFRADPSRAVRVLRAARIDACSLANNHTLDFEEEGLLD
                     TLEYLDGAGIRHSGAGRDLEEAARPAILDGPDGEKVALLAFTDNEPAFAAGVDRPGTN
                     YLPTSLEPEVLDRVEAAIAGAREAGADTILFSNHWGPNMVERPRDIFRRFARSVVDRG
                     ADVYYGHSAHVFQGVEVYRGKPILYDTGDFIDDYAVDERLRNDRSFVFLASVEGGTFQ
                     RLELFPVALSFAQTGLATGGEREEILRRMERLSAELGTTFVRHEGALVLEGS"
     gene            complement(1403..2044)
                     /locus_tag="VNT12_09555"
     CDS             complement(1403..2044)
                     /locus_tag="VNT12_09555"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWI46403.1"
                     /translation="MGSVSDPEQLVRAAAAAGVGDPRVLDAVRTVPRAGFVPPHLAGR
                     AYLDEPLPIPRGQVTTQPSLVAKMVEALGLTGSGRVLEVGAGYGWQTALLARLCGFVW
                     AVERFADVAEAARENLTRFGVANAEVVQGDGTEGLSEHAPYDAILVAAAFPSVPRPLE
                     EQLVAGGRLVQPVGPGGREEVVLFEKGPRNLVRLQVVAGASFVKLHGAHAFPE"
     gene            complement(2148..3089)
                     /locus_tag="VNT12_09560"
     CDS             complement(2148..3089)
                     /locus_tag="VNT12_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWI46404.1"
                     /translation="MDLRINSKSHVPVHVQLEGQLKHLILTGSFEVGSRLPSIRAMAG
                     FLRVNRNTVARVFSDLEREGYVESRRGSGVYVVEPPVEAEDLRRQEVLERVMDLAAAQ
                     GVPVEDLAYALLARAGAKPQEKTPILFVECTRAELDQFSSELEEQLPVEVERVLLEDL
                     SERVSGAVDLPWRLAVTTFFHIHEVQDIVEPHGIEAVALLAEATLESLQRLMELPSGT
                     PVGVVGWGRTCMENLSRSIEGAGLDHLDFVQVYIDEDPKEVLATLGSVKSVVCASVTA
                     RRLRELGIPEDLEIIEEDRILDQGGIEMLGRMLRQVL"
     gene            3369..3968
                     /locus_tag="VNT12_09565"
     CDS             3369..3968
                     /locus_tag="VNT12_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWI46405.1"
                     /translation="MSKNTGETEGRISFTLAGALAGARRCVPIAISGCGIGLVFGTLA
                     GQAGLGAGEAVLMSALVFSGAAQFVVLGLWAVPLPVATIVLATLVVGLRHVLMGAVLA
                     PVFSKLPRLKAYGSVFFMLWLASRFDLSERAGAWLDQIPGAILVSLVAPTILMGGPVE
                     VFAALVVVIVSLRTGSLPAAMVTGVGTVLALRALEANLL"
     gene            complement(3992..>4470)
                     /locus_tag="VNT12_09570"
     CDS             complement(3992..>4470)
                     /locus_tag="VNT12_09570"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWI46406.1"
                     /translation="GNGRLSFVPVLLTATAARVCAALVMYAVGFRLKEARLRRVIKHF
                     ERSKLLFVSDFERASAVFERHGGKAILVGHLIPGVGALVSVPAGLKRMPLRWRFLGYT
                     ILGCSIWNASLIGLGWALGGRWRIIEVFTSFVGSAILIILIAATVWFFWRGRKAHR"
BASE COUNT          802 a         1452 c         1428 g          788 t
ORIGIN      
        1 tacgagtgca gctatgagat ccaccgtggc ctgacggaga tgtacgtcag cgacgagcga
       61 ttccgctcca actacgacgc gacgacgccc ggtctggccg ctttcgtcag ggatgcggcc
      121 cacgccaatg ccagccgggc cgaacctggc agctagggga ccaagccgtc ctcaagcggg
      181 gcgcacgcgc tttcagggcc gggagcattc ccggccctgg gtggtccggc ggttggaggg
      241 gacacgcttg gtaggaggtc ctcggtatga gagacttcgg gggaaatgag ctggccgtcc
      301 gagagctcag agccgtttga caagccggcc cacggcgcgg ggtttcgacg ctggaagatg
      361 aacatgggtc gagtgggatc gggcctcgga tgatcgccct ggggctggtc ggcgacgtga
      421 tgctcgggcg cggggtcgac gaggcgctca ggggcatgcg gcccgacgaa ccctggggcg
      481 atgcattgtc cgtgcttgac gccgccgacc tccgcgtcat aaatctggag tgtgcgatca
      541 cgacccacga gaacccgtgg agccgcacgc caaagacctt ccacttccgg gcggacccct
      601 cccgcgccgt cagggtcctg cgcgcggccc ggatcgacgc ctgctccctg gccaacaacc
      661 acaccctgga cttcgaagag gagggactac ttgacacctt ggagtatctg gatggcgccg
      721 gcatcaggca ctccggtgcg gggcgcgatc tcgaggaggc cgctcgtccg gccatcctcg
      781 acgggccgga cggagagaag gtcgccctac tggctttcac cgacaacgaa cctgctttcg
      841 ccgctggagt agaccgtcca gggacgaact acctgccgac ttctcttgag ccggaggtcc
      901 tggatcgggt ggaggcggcg atagcgggcg cgcgcgaagc gggggcagac acgatcctgt
      961 tctccaacca ttggggtccg aacatggtgg agcgtccaag ggatatcttc cgccggttcg
     1021 cccgctccgt cgtggaccgg ggggctgacg tgtactatgg gcacagcgcc cacgtcttcc
     1081 agggagtgga ggtataccgg ggaaagccca tcctctatga caccggcgac ttcatcgacg
     1141 actacgcggt ggacgagagg ttgcgcaacg accggtcctt cgttttcctg gcctcggtgg
     1201 agggcggcac gtttcagcgc ctggagctgt tcccggtcgc gctctccttc gcccagacgg
     1261 ggctcgctac cggaggggaa agggaggaga tcctgcgccg catggaacgc ctctccgccg
     1321 agttgggcac caccttcgtc cgccacgaag gcgccctggt gctcgaaggc agttagatct
     1381 ctcggacgcc tctgaggacc tactactccg ggaacgcgtg tgcgccatgg agtttaacga
     1441 agctggcccc ggcaacgacc tggaggcgca cgagattccg aggtcctttc tcgaagagga
     1501 ccacctcctc cctgccgcca ggaccaacgg gctgcaccag gcgtccccct gcgacaagtt
     1561 gttcctcgag cggccggggg acggaaggga acgccgcggc gaccaggatg gcgtcgtagg
     1621 gggcgtgctc ggaaagaccc tctgtcccgt cgccctggac tacctcggcg ttcgccacac
     1681 cgaaccgggt gaggttctct ctcgcggctt cggctacgtc cgcgaagcgc tccaccgccc
     1741 acacgaagcc gcacaggcgg gcgagcagcg ccgtctgcca cccgtagccc gcgccgacct
     1801 ccagcacccg tcctgaaccc gtaagcccca gggcctccac catcttcgcc acaagagagg
     1861 gctgcgtggt cacctgcccg cgggggatgg gcagtggctc gtccaggtag gcgcgcccgg
     1921 cgaggtgagg cggaacgaag ccggccctgg gaacggtgcg cacggcgtcg aggacgcggg
     1981 ggtcgcctac ccccgcggcg gcggcggcac ggacgagttg ttccggatca cttacggaac
     2041 ccattatgcc tgggggctcg gcgcgaagga gcgtcgccga tagacccgcg agcagcgggt
     2101 acacactagt ttggacgtag gtgatggcat ccttgccaag atgcccatca cagcacctgg
     2161 cggagcatcc ggccgagcat ctcgataccg ccctgatcca ggattcggtc ttcttcgatg
     2221 atctccagat cctctgggat accgagttcg cggagccgtc tggccgtgac gctggcgcac
     2281 accacactct tgacgctgcc gagggtggca agcacttctt taggatcctc gtctatgtag
     2341 acctggacga agtccagatg gtcgaggccc gccccttcga tagaacgcga gagattctcc
     2401 atgcacgtgc ggccccagcc cacgaccccg accggagtcc ccgacggaag ctccatcaac
     2461 ctctgcaggc tctccagcgt cgcctcggcc agtagcgcga ccgcctctat cccgtgcggc
     2521 tccacgatat cctgcacctc gtggatgtgg aagaacgtcg tcacggcgag tcgccaaggc
     2581 aggtctacag cgcccgagac cctctcggag aggtcctcga gcagaactct ctctacctcg
     2641 acagggagct gttcctcgag ctcggaggag aactgatcca actccgcccg cgtgcactcg
     2701 acgaagagga tgggagtctt ctcctgcggc ttcgcaccgg cccgtgcgag caacgcgtac
     2761 gccagatctt cgacaggcac gccctgcgcc gccgcgagat ccataaccct ctccaagacc
     2821 tcctgacgcc tcaagtcctc cgcctccaca ggaggctcta ccacgtagac gccactgccg
     2881 cggcggctct ccacgtaacc ctcccgctcg aggtcagaga acacccgcgc caccgtgttc
     2941 ctgttcaccc gtaaaaatcc agccatcgcc ctgatgctcg gcaaccggct cccgacctcg
     3001 aagctgcccg tcagaatgag gtgcttgagc tgcccctcca actgtacgtg tacaggtacg
     3061 tgactcttcg aatttatccg taaatccatt aattaaccta ttgtcctatg acaccgagcc
     3121 aattataatt gtcccaagtc agcgtgacaa ttatagcgca cccggcgagc atggaggtga
     3181 gtcatgggta ctctgttcga tgtggagctt gcgagggaac gacggtacga gctattgcac
     3241 gaagcggagg agcgtaggct cgctcgtgct ttgcggaggt tccgtcaggg tgacggggaa
     3301 gatcaccgcg ccgagaagga gcctacagtg tcgtgccact ctacgtgatg ttgcgtagat
     3361 aggacatcgt gtcgaagaat accggagaga cagagggccg gatctcgttc acccttgcgg
     3421 gggcgctcgc aggggcgagg aggtgcgtac ccatagcgat cagcggttgt ggcatcggcc
     3481 tcgtgttcgg gacgctggcg ggacaggctg gtctaggcgc tggcgaggct gtcttgatga
     3541 gcgctctcgt gttctcgggg gcggcacagt tcgtcgtact tgggctctgg gcggtcccgc
     3601 tgccggtagc tactatcgtc ctcgctacgc tcgtcgttgg tctcaggcac gtgctgatgg
     3661 gcgcggtcct cgcgcctgtg ttctcgaagt tgccgcgtct caaggcctat gggtccgtgt
     3721 tcttcatgct ctggctcgcg agccggttcg acctctccga gcgggccggg gcgtggctcg
     3781 atcagatacc gggtgcgatc ctcgtctcgc tcgtcgcccc taccatcctg atgggcgggc
     3841 ccgtcgaagt gttcgccgca ctcgttgtcg tgatcgtctc cctcaggaca ggcagcctgc
     3901 cggctgcgat ggtgacaggg gtagggaccg ttctcgcgct gcgagccctg gaggcgaacc
     3961 tgctttgacc tcgcgcgctc atgcactccg tctacctgtg cgccttccgc cctcgccaga
     4021 agaaccagac cgttgcggcg ataagaatta tcaggatcgc agacccgacg aaagacgtga
     4081 agacttctat tatcctccac cggccgccga gagcccaccc caggccgatc aaactcgcgt
     4141 tccagatgct gcatccgagg atcgtgtagc caaggaaccg ccagcgcaga ggcatgcgct
     4201 tgaggccggc gggcacagag accagggcgc cgacgccggg gatcaagtgt ccgacgagga
     4261 tcgcttttcc cccgtgacgc tcgaataccg cactcgccct ttcgaaatca gacacgaaaa
     4321 ggagcttcga acgttcgaag tgcttgatca cacgccgcaa gcgcgcttct ttgagcctga
     4381 atccgacggc gtacatgacg agcgcagcac ataccctcgc cgcggtcgcc gtgagcagca
     4441 cagggacgaa cgagagacgt ccgttgccga
//
LOCUS       DATMJA010000400         3113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_349497, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000400 DATMJA010000000
VERSION     DATMJA010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349497"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VNT12_09575"
     CDS             <1..1033
                     /locus_tag="VNT12_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HWI46407.1"
                     /translation="NRPNVAGTKRGTGEGRSILLNAHTDTVDPGDSTAWKEDPLSGNL
                     EGDLLYGRGSCDMKGGLVTHLVALDALSDLGLGLRGDVTVAATVGEENGGLGALSTVL
                     RGYRADAAIITEPTRLALVPAQGGSLVFRLKIPGRSAHAAVREEGVSALEKFVPIFEN
                     LRELEQERNDVLSHPLYDGVSNKVPINIGVVRAGNWASTVPESLVAEGRVGLIPGEEV
                     EPFKDLVSERIATVAERDSWLREHPPELEWFGGQFAPAEVPPDAPICEAVKRAHGRAT
                     GEKPAVEGVPYGADMRLFIHFGDMPCVMYGAGDVNVAHAPDEHISVTELLTATSTVAC
                     LLADWCGVA"
     gene            complement(1101..2756)
                     /locus_tag="VNT12_09580"
     CDS             complement(1101..2756)
                     /locus_tag="VNT12_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWI46408.1"
                     /translation="MATQTTESGGAGAEHKLKREVSSVGLLFVSLGSIIGSGWLFGAL
                     TSAQIAGPAAIISWILGALVMLVLALAHAELGSMYPVAGGSTRYPHFAFGSLAGFVIG
                     WAVWIGAVTVAPIEVLAALQYLTHYFHFLTDTTGGVTVLTGIGIVISVVLMAVFTVIN
                     LIGIRLLAESNNIIMIWKVAIPFLAVAVIMILSFNTSNFTAADGFAPFGIEGVLSALS
                     VGGIIFAYQGFEQAIQLGAETRNPERNIPLAVIGSMVVGVVLYILLQVAFIGAIDPQE
                     DLKNGWAEISFPGEAGPFAGLATLVGAGWLATLLYIDAFVSPAGTGLIYTASSSRLSY
                     AFARNHYIPRQFGFINNRGVPIVSIIFSFLVGCFMFLPFPGWQQLVGFIIAATVIGYA
                     AVPLALGALRRQEPDHPRPFKLPAGELLAILSFIVANLVIYWSTWDVIWHVLIAMVLG
                     LVLLGIGHIVNPSELTPDLDWRGASWLWPYFIGLAVLSYLGPEDFGGIEVIPFGWDML
                     VMAIFSVAIYYYAMSVRLPPEEVRGHVADAREEVEQVEEEVAI"
BASE COUNT          627 a          986 c          977 g          523 t
ORIGIN      
        1 gaatcgaccg aacgtcgccg gcacaaagag agggacaggg gaaggacgct ccatcctgct
       61 caacgcccac accgacaccg tggacccggg agactcgacc gcgtggaaag aggatccact
      121 ctcgggcaac ctggagggcg acctgctcta cggacgcggc tcctgcgaca tgaagggcgg
      181 gctcgttacc cacctcgtcg ccctcgacgc cctctccgac ctcggtctcg ggttgcgcgg
      241 cgacgtcacc gtggctgcga cggtagggga ggagaacggg gggcttggcg ccctctcgac
      301 cgtcctgcgc gggtaccgcg ccgacgccgc catcatcacc gagccaaccc gtctcgccct
      361 cgtgcctgcc cagggcggtt cgctcgtctt ccggctgaag atcccaggcc gctcggccca
      421 cgccgccgtc cgcgaggagg gcgtctcggc gctggaaaag ttcgtcccca tcttcgagaa
      481 cctacgcgag ctggagcagg agcgcaacga cgtactcagc cacccgctct acgacggagt
      541 aagcaacaaa gtgcccatca acataggcgt cgtaagggcc ggtaactggg cctcgaccgt
      601 ccctgaatcc ctcgttgcag agggccgcgt cggcctgatc ccgggcgagg aggtagagcc
      661 cttcaaggat ctcgtgtccg aacgcatagc caccgtcgcc gaacgcgact cgtggctgcg
      721 cgaacatccg ccggagctcg agtggttcgg tggacagttt gcccccgccg aggtgccgcc
      781 cgatgcccca atctgcgagg ccgtaaagag ggcccacggg cgggcgacgg gcgagaagcc
      841 ggccgtcgag ggtgtcccct atggcgctga catgcggctg ttcatccact tcggggacat
      901 gccgtgcgtc atgtacggcg ccggtgacgt gaacgttgcg cacgcgcctg acgagcacat
      961 cagcgtcaca gagctcttga ccgcgaccag tacggtagcc tgcctcctgg ccgactggtg
     1021 cggcgtggca tgaccacagg ctctcttccg gacagagggc tagggacggg tcagcctgcc
     1081 cctagcccct cctggcagcg ttagatcgcg acctcctctt ccacttgctc tacttcctcc
     1141 ctcgcatcgg cgacgtggcc ccgcacctct tcgggcggaa ggcgcaccga catagcgtaa
     1201 tagtagatgg cgacgctgaa gatcgccatg accagcatat cccagccgaa cgggatgact
     1261 tcaatgccgc cgaaatcctc aggaccaagg taggaaagta ccgcaagccc gatgaagtac
     1321 ggccacagcc aggatgcgcc tctccagtcg aggtccgggg tcaactccga agggttgacg
     1381 atgtgaccta tgccgaggag cacgaggccg aggaccatcg cgatgagcac atgccagata
     1441 acgtcccagg tgctccagta gatgaccagg ttcgccacga taaaggagag gatcgccagt
     1501 aactcgccgg ccggcagctt gaatggccgc gggtggtcgg gctcctgccg cctcaacgcg
     1561 cccagagcaa gcggcaccgc cgcgtacccg attacggtcg ccgctatgat gaagccgacg
     1621 agctgctgcc agcccgggaa cggcaggaac ataaagcagc cgaccagaaa ggagaagatg
     1681 atgctcacga taggaacacc cctattgttg ataaagccga attgcctagg gatgtagtgg
     1741 ttgcgggcga aggcgtaaga gagacgcgaa ctcgaagcgg tgtagatgag gccggtgcct
     1801 gccggggaga caaaggcgtc tatgtacagg agcgtcgcca gccagccggc gcccaccagg
     1861 gtggccagcc ctgcgaaggg accggcctcc cccgggaacg agatctccgc ccacccgttc
     1921 ttgaggtctt cctggggatc gatggcacca atgaaagcga cctgaagcag gatgtacagg
     1981 acgactccga ccaccatcga cccgatgacg gcgagtggga tgttgcgctc cgggttgcgg
     2041 gtctctgctc cgagctggat ggcctgctcg aagccctggt aggcgaaaat aattcccccg
     2101 accgaaagcg cgctcagcac gccctcgata ccgaacgggg cgaatccgtc ggcggcggtg
     2161 aagttggagg tgttgaagga caggatcatt atgacggcga ccgccaggaa ggggatcgcg
     2221 accttccaga tcatgattat gttgttcgac tcggcgagca ggcggatacc gatgaggttg
     2281 atgaccgtga acacggccat gagcaccacg gagatgacga tgcctatccc cgtcaggact
     2341 gtcacgccgc ccgtcgtgtc agtgagaaag tggaagtaat gggtgaggta ctgcagcgcc
     2401 gccagcacct cgatgggcgc cactgtgacg gccccgatcc acacggccca tcctattacg
     2461 aaccctgcaa gactgccgaa cgcgaagtgc ggataccggg tcgatcctcc ggccaccggg
     2521 tacatgctac cgagctcggc gtgggccagc gccagaacga gcatcaccaa agctcccagt
     2581 atccacgaga ttatcgctgc ggggccagcg atctgcgccg aggtcagggc gccgaagagc
     2641 catccggacc cgatgatcga tcccagcgac acgaagagca gcccgaccga gcttacttct
     2701 ctctttagct tgtgctctgc ccctgctccc cctgattccg tagtttgtgt agccattggc
     2761 aagcctcctc gcttagcgca aaagatccgt caagacttat ccttgtccgc tcgttccggt
     2821 ctaggtccga tccgaagtcg gtatgcaacc gtgccgaggc cttccgtcat ggccaggtcg
     2881 cgtaggttct ctcggcgatc agtttttaat cctccttccc tctggaaagc tgttggcatt
     2941 gtgggccatt agtcggtccg gagcaagtac tcgccgggca ggaagattgc cgggtctccg
     3001 atactgcgga atcttgatag aagtcaagat caggacggta cggcggacgt actctcgggc
     3061 taggagaggg cggctacgat cctgtcgttc tcagccgtgc tcccgagcga gat
//
LOCUS       DATMJA010000401         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_823450, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000401 DATMJA010000000
VERSION     DATMJA010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823450"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VNT12_09585"
     CDS             complement(<1..481)
                     /locus_tag="VNT12_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWI46409.1"
                     /translation="MRQETEKGYFGPFGGRYVPETLIHALEELEEAYERYRDDPEFGE
                     ERDRLARDFVGRPTPLMFAERLTRKWGGAKVWFKREDLAHTGAHKINNTLGQILLAER
                     MGKERIIAETGAGQHGVATATVSALYGKECIVYMGEEDTRRQRLNVVRMKLLGAEVVP
                     "
     gene            complement(465..1079)
                     /locus_tag="VNT12_09590"
     CDS             complement(465..1079)
                     /locus_tag="VNT12_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWI46410.1"
                     /translation="MTLVKICGITNAEDARVAADAGADAVGLLFAESPRRVTVEEARR
                     ISLALPENILRVGVFVDEEPAEILRISREVGLDLAQLHGDETPETVTAVRRGGVKVMK
                     ALRVRDALSLEALDRYEADLILLDAHSERARGGTGQRFEWEVAKSSTGRDNIVVSGGL
                     DPENVREAVEFFEPYGVDASSSLEDGPGRKNGERVRRFVLAARD"
     gene            1301..>2327
                     /locus_tag="VNT12_09595"
     CDS             1301..>2327
                     /locus_tag="VNT12_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HWI46411.1"
                     /translation="MRPEDIIARAAEVLRENDMGEWTRASPTLYPHQWSWDTAFIAIG
                     LARLDTGRAAREIRTLLQYQWKNGKIPHIVFNPDAPPESYFPGPEHWASAADSPDAPS
                     GPSLTSGLCQPPVHAIAARHILSVAEARGEGVAEAREFLGEVYPKLLAWHRYLATARD
                     PEGSGLVTIYHPWESGTDNSPRWTSALERVEVGEIPDYERLDLDHIPDPAERPTGYEY
                     DRFIWLVEIIKQGRCDDAVLYRTSPFLAKDVLASAILVRANEALLDVADVVGALDEEG
                     TMIRAWIERGRKGLDERWDPGLGLCLDYDLRADEPLAARTIAGFAPLIAGTGGAERWD
                     KLLKVFES"
BASE COUNT          393 a          773 c          720 g          441 t
ORIGIN      
        1 cgggcacgac ctccgcaccg agtagcttca tccgcacgac gttcagtctc tggcgccggg
       61 tgtcttcttc gcccatgtag acgatgcact ctttgccgta gagagctgag acggtggccg
      121 tggcgacacc atgctggccc gcgccggtct ccgcgattat gcgttccttg cccatcctct
      181 cggcgagcaa tatctggccc agggtgttgt tgatcttgtg cgccccggta tgcgcaaggt
      241 cctcgcgctt gaaccagact ttggccccgc cccacttgcg ggtcagacgt tcggcgaaca
      301 tgagaggggt cggccttccg acgaagtccc tcgccaggcg gtctcgctct tcgccgaact
      361 cggggtcgtc cctgtagcgt tcgtaggcct cttcgagctc ttcgagggcg tggatcaggg
      421 tctcgggcac gtagcgcccc ccgaaggggc cgaagtaacc cttttcagtc tcttgccgca
      481 aggacgaacc tccgcacccg ctcaccgttc ttcctgcctg gcccatcctc cagggaactc
      541 gacgcgtcca ccccgtaggg ttcgaagaac tcgaccgcct cccgcacgtt ctcagggtct
      601 agccctccgg agacgacgat gttatcacgt ccggtggagg acttcgctac ctcccactcg
      661 aacctctggc ctgtgcctcc ccgcgccctt tcgctgtgcg cgtcgagcag aatcaggtcg
      721 gcctcgtatc tgtccaacgc ctcgagggag agagcatctc ttacccgcag tgctttcatg
      781 actttgacgc caccccgccg tacggccgtt acggtctcgg gcgtctcgtc gccgtggagt
      841 tgcgcgaggt caagcccgac ctcgcgggag attctgagga tctcggccgg ctcctcgtca
      901 acgaacacac cgaccctgag gatgttctcg ggtaatgcca aggagattct gcgggcctct
      961 tcgacggtca cgcgacgcgg gctctcggcg aacaggagtc cgacggcgtc ggcccctgcg
     1021 tccgcggcca cgcgggcgtc ctctgcgttg gttatgccgc atatcttgac cagcgtcata
     1081 acccgaagtg taccccttat cgccggcggt gagggttcgg atctcgggga ggatccaggc
     1141 gcgccacgcg taccgttaaa gatttcgtag tgtgcgggca atcgcggcgt aagatttgcg
     1201 ttatggatta ctattcgcgg gtggagcgcc gagaaagcgc tgctgacagc ctggccatgc
     1261 gcagggacgg gtgggaggac tatagccgga aggagtctgt ttgcgacccg aagacatcat
     1321 cgcgcgggct gcggaggtgc tgcgggagaa cgacatgggc gaatggaccc gcgcctcccc
     1381 cactctctac ccgcaccagt ggagttggga cacagccttt atagccatcg ggctggcccg
     1441 cctcgacact ggacgtgccg cccgtgagat ccggaccctc ctccagtacc agtggaagaa
     1501 cgggaagata ccgcacatcg ttttcaaccc cgatgcgcct cccgagagtt acttccctgg
     1561 tcccgagcac tgggcgagtg ctgccgattc ccctgacgcc ccgtccggtc cgtcccttac
     1621 cagcggcctc tgccagccac cggtacacgc gatcgcggcc aggcacatac tcagcgtcgc
     1681 cgaggcgagg ggcgaggggg ttgccgaagc cagggagttc ctcggcgagg tctaccccaa
     1741 gctccttgca tggcacaggt acctggcgac cgcccgcgac ccggaaggtt ccggacttgt
     1801 gaccatctac catccctggg agagcggcac agacaactcc ccacgctgga cctctgcgct
     1861 ggagcgggtc gaggtcgggg agatacccga ctacgagcgc ctcgacctgg atcacatccc
     1921 cgaccccgcc gagcggccga cgggctacga gtacgaccgc tttatctggc tggtcgagat
     1981 catcaagcaa ggccgttgcg acgacgccgt tctttaccgg acgagcccgt ttctggccaa
     2041 ggacgtgctg gcgagcgcga tcctggttcg ggcgaacgag gcacttctcg acgtggccga
     2101 tgtcgtcggc gctcttgatg aagaaggaac gatgatccgg gcctggatcg aacgcgggcg
     2161 aaaagggctg gacgaacgtt gggaccccgg actcggtctt tgcctcgact acgacctgcg
     2221 cgccgacgaa cccctcgcgg cacgcaccat cgccggcttc gcaccgctca tagccggtac
     2281 aggtggtgca gagcgttggg ataagctact caaggtcttc gagtctc
//
LOCUS       DATMJA010000402         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_231326, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000402 DATMJA010000000
VERSION     DATMJA010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231326"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..>2505
                     /gene="ileS"
                     /locus_tag="VNT12_09600"
     CDS             277..>2505
                     /gene="ileS"
                     /locus_tag="VNT12_09600"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWI46412.1"
                     /translation="MKFDKVSPQVDFPRLEEEILGLWKKTNAFENSVHGRPEDRPFVF
                     YEGPPTANGRPGFHHALARAFKDLIPRYKTMQGYRVERKGGWDCHGLPVEIQVEKELG
                     LDGKADIERYGVEEFNRLCRESVFRYVEDWQKMSERLGFWVDMDDPYRTLDDSYVESV
                     WWALKTLYEKDLLYEGFKVSPHCPRDQTSLSSAEVALGYQQYPDPVVDPSIYVRFPLR
                     DEEHTSLLVWTTTPWTLVSNAAVAVGPDVEYATVEVDGERVILAEELLVKVLGEDGYR
                     VVSKTKGRNLEGTRYRRPFDYVPVERTEDYKNLWTVVLGDYVTTTEGTGLVHTAPAYG
                     EEDARTARRYGLPTIHPVRPDGTFDERVGPFAGQFVKDADAGLVEELSEKGLLFRAEE
                     LEHAYPHCWRCGTPLLYYAKKAWYGRTTAVLDELLSENEKINWVPEHIKWGRFGDWLK
                     NNIDWALSRERYWGTPLPIWRTTSGDTVVVGSKRELGELAIDPVPDDLHRPYIDGVRV
                     RHPETGEEATRVPEVLDVWFDSGSMPFAQWGYPHGEASEERSRSQFPADYICEAVDQT
                     RGWFYSLLAVSTMLFGKSSYRNCLVLGHILDAEGKKMSKSLGNVIDPWDVFEKQGADA
                     LRWALYTATSPGNTRRFSRGQVDEAVRKYLLTLWNTYSFFVTYARIDGFDPEEDYVEP
                     EGRSLMDRWALSELQLTVRTVTERLDAYDVTAAGRAVGGFVDELSNWYVRRSRRRFWK
                     GED"
BASE COUNT          495 a          765 c          838 g          407 t
ORIGIN      
        1 gtggaagccc ggcgcggcac gcggcccgaa catcctccca gagctgccgg ccgaaagcgg
       61 aaggcgagag tagcctagac ggttagtagg accggccggg cgagcccgtt acagcgcgtg
      121 cgttccggga gaccgcgacg ccactgagag gaccggaccc gtgaggttcc ggacaagtag
      181 ggtggtaccg cgagggcctc atgcaggctc ccgtccctac ggtggagcga cgctccgccc
      241 tgggggtggg agcttttgta cgaggaggtc agcggcatga agttcgataa ggtcagccca
      301 caggtagatt ttccgaggct cgaggaggag atcctcggtc tctggaagaa aacgaacgct
      361 ttcgagaatt ctgtgcacgg caggcctgag gacaggccct tcgtcttcta cgaggggccg
      421 ccgacggcca acggcaggcc ggggttccac cacgcgctag caagggcttt caaggatctc
      481 atcccccggt acaagaccat gcagggttac cgggtcgagc gcaagggcgg ctgggactgc
      541 cacggtctgc ccgtcgagat ccaggtagag aaagagctcg gcctcgacgg caaagcggat
      601 atcgagcgct acggcgtcga ggagttcaac cgcctctgcc gcgagtccgt cttccgttac
      661 gtggaggact ggcagaagat gagcgagcgg ctcggcttct gggtggacat ggacgatcct
      721 taccgcacgc tcgatgactc atacgtcgag agcgtctggt gggccctgaa gaccctctac
      781 gagaaggacc tcctctacga gggtttcaag gttagccccc actgcccccg cgaccagacc
      841 tccctctcct ccgccgaggt cgccctcggc taccaacagt accctgaccc cgtggtggac
      901 ccgagcatct acgtgcggtt tcccctccgg gatgaggagc acaccagcct cctcgtctgg
      961 accacgacac cgtggaccct cgtctcgaac gccgccgtcg ccgttgggcc cgacgtggaa
     1021 tacgccaccg tagaagtaga tggcgagcgt gtgattctgg ccgaagagct actcgtgaag
     1081 gtgctcgggg aggacggcta cagagtagtc tccaaaacca aagggcggaa cctggaaggc
     1141 acgaggtacc gccgtccctt cgattacgta cctgtggagc gaaccgaaga ctacaagaac
     1201 ctctggaccg tcgttctcgg ggactacgtg acgaccacgg aggggaccgg actcgtgcac
     1261 acggcgccgg cttacgggga ggaggacgcc aggacggccc gcaggtacgg actgccgacg
     1321 atccaccccg tcaggcccga cggtacgttc gacgagcggg tcgggccttt cgccgggcag
     1381 ttcgtcaagg acgcggacgc gggtctcgtc gaggagttga gcgagaaggg gctgttgttc
     1441 agggccgagg agctggagca cgcctacccg cactgctggc ggtgcgggac gcctttgctg
     1501 tactacgcga agaaggcctg gtacgggcgg accaccgcgg tgctcgacga gcttctatcg
     1561 gagaacgaga agataaactg ggtccccgag cacataaagt ggggacgctt tggggactgg
     1621 ctgaagaaca acatagactg ggccctgagc cgcgagcgct actggggcac gccgctcccg
     1681 atctggcgca ccacttccgg cgatacggtc gttgtcggca gcaagcgaga gctgggggag
     1741 ctcgccatag accccgtccc agatgacctg cacaggcctt acatagacgg ggtgcgcgtg
     1801 aggcaccccg agacgggcga ggaggccacc cgcgtccccg aggtcctcga cgtgtggttc
     1861 gactcgggca gcatgccttt cgcccagtgg ggctatccgc acggcgaagc cagcgaggag
     1921 cgttcccgga gccagttccc cgccgactac atctgcgagg ccgtcgacca gacacggggg
     1981 tggttctact cgctgctcgc cgtctcgacg atgttgttcg gcaagagctc gtacaggaac
     2041 tgcctggtcc tggggcacat cctcgacgcc gagggcaaga agatgagcaa atctctcggc
     2101 aacgtcatag acccctggga cgtcttcgag aagcagggcg ccgatgctct gcgctgggcg
     2161 ctctatacag ccaccagccc aggcaataca aggcgcttct ccaggggcca ggtcgacgag
     2221 gccgtccgta agtatctcct tacactctgg aacacctact ctttctttgt gacctacgcc
     2281 cgcatcgacg gctttgatcc cgaggaggat tacgtcgagc ccgagggacg aagcttgatg
     2341 gaccggtggg ccctcagtga gctgcaactc accgtgagga ccgtgaccga gaggctcgat
     2401 gcctacgacg taacggcggc aggaagggct gtcggagggt tcgtggacga gctctccaac
     2461 tggtacgtga gacgcagccg caggcgcttc tggaagggag aagac
//
LOCUS       DATMJA010000403         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_53813, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000403 DATMJA010000000
VERSION     DATMJA010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53813"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1750)
                     /locus_tag="VNT12_09605"
     CDS             complement(377..1750)
                     /locus_tag="VNT12_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWI46413.1"
                     /translation="MAAGPEPCRQTWRVADFTRTLRWEELSAGVRSQAIRCVLDLCGA
                     AVAGSRTEAAQTAATYALYAHGNGSSTIIGTGANSTPVGAALANGFAASALDIDDGYR
                     PVKGHPGAVVFPAVLAAAEEAKSSGVELLTALVVGYEVAMRAGRILHLLYDFYHGSGA
                     WAPLGAAAGTARLLGCSAEQTWHTLGIAEFHAAMTPEMRSVEHPSMLKDGIGWGAMVG
                     LASAQLAARGFTGFPALFDAGDVGPHFTESLGDEYLILDLYFKPYACCRWAQPAIEGA
                     LSAAHQLSATAADVARVRVHTFEAATHLRSAAPRTTEEAQFSLPWTVACALIDGAVGP
                     EQVAGKSLTDPARRALAALVEVVVDAELEEAFPEQALAWVEIETTDGSRARSGIFSAP
                     GDAHTPFSDTELTEKFRALTEPVLGSHRAKKLASAIERLPDSPNLDEITDLLRCAASP
                     DARIQSG"
     gene            complement(1751..>2485)
                     /locus_tag="VNT12_09610"
     CDS             complement(1751..>2485)
                     /locus_tag="VNT12_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWI46414.1"
                     /translation="VFPELSLSGYALGELSDDVPIEAHSKPIVSLAEAAGEMAVVVGF
                     CEEGPRFQTYNSAAYLEGGSIRHLHRKLYLPNYRIYEERKHYNPGQSMRAFDTRLGRM
                     AMLICNDAWQTFLPFIAVQDGAQVLIIPADSGSYPSYPELLDTRGYWRGITRFYSRML
                     ECYVVFVNRVGKQDNLVFWGYSHVVDPWGTVVAEGPLHEEALIAVDIDLGNVRRRRRE
                     VPLVKEARLALLARELNRLAEEGGDL"
BASE COUNT          449 a          777 c          795 g          464 t
ORIGIN      
        1 ttcatccact gactcaccgc acccttgctc acgcccagag cgcgagctat atcctgctgc
       61 ttccagccct cttgcttgag ctcccacgca cgtagccgtc ggccttcacg ccactcggtt
      121 gcttgctggc tcgataccgt cttttccata cggctcatgg tctcaaagtt tagatgcttc
      181 cgcagagatc agtaggtgca aagaccccca accagaagga tcaagagaca cctcgccgag
      241 tgagttgcca gggtacctgg ctgggagaat attgcacaag gctggttggt tatctcgccc
      301 ggtacagtcg tggggacgcg atcctggcga ggagttttgg gtggaggggc gtcggatgcg
      361 ggagatgaac tcggacttag cccgattgta tccgcgcgtc gggggatgcg gcgcatcgga
      421 gcaggtcggt tatctcgtcg agattcggag aatcgggaag cctctcgatg gcagaggcca
      481 gcttcttggc gcgatgggag ccgagcaccg gctccgtgag ggccctgaac ttctctgtaa
      541 gttccgtgtc gctgaaaggc gtgtgtgcat cgcccggtgc tgagaagata cccgagcggg
      601 cacggctacc atccgtcgtt tctatctcca cccaggcgag agcttgctcc gggaaagcct
      661 cttcgagctc cgcatcgacg acgacttcca ccagcgccgc tagcgcccga cgggcgggat
      721 cggtaaggct tttcccggcc acttgctccg gtcctactgc accgtcgatc agggcacagg
      781 caaccgtcca gggcaggctg aactgggcct cttccgtcgt acgtggagcc gcggagcgaa
      841 ggtgcgtagc cgcttcgaag gtatgcactc gcaccctcgc aacgtcggcg gccgtagcgc
      901 tgagctggtg cgcagccgaa agcgctccct cgatggctgg ctgtgcccac cggcaacagg
      961 cgtatggctt gaagtacagg tcaaggatca ggtactcgtc tcccaagctc tcggtgaagt
     1021 gcggtccaac atcgcctgcg tcgaatagag ccggaaagcc ggtaaatccg cgagcggcca
     1081 gttgagcgga agcgaggccg accatggcgc cccacccgat tccgtccttc agcatactgg
     1141 ggtgctccac cgaccgcatc tcgggcgtca tggccgcgtg gaactctgcg ataccaaggg
     1201 tatgccacgt ctgctctgcg ctgcatccga gcaaacgcgc ggtgccggcc gccgcaccga
     1261 gaggcgccca ggctccgctc ccgtggtaga aatcgtagag caggtgcagg atgcggccgg
     1321 cgcgcatagc cacttcatag ccgactacca gggcagttag gagctcaacc ccgctggact
     1381 tcgcctcttc ggcggcggcg agcacggccg gaaatactac cgcgcccggg tggcccttaa
     1441 cgggacggta tccgtcgtcg atgtccaagg cggaggctgc aaacccgttg gctagggccg
     1501 cgccgaccgg tgtgctattt gcgccggtgc ctatgatggt actcgaccca ttaccgtgag
     1561 cgtaaagagc gtacgtggcc gcggtctgcg ccgcctcggt gcggctgccc gcaacggcgg
     1621 ccccgcagag gtcgagcacg cagcggatag cctgggaccg cacgcctgcc gagagctctt
     1681 cccaacgcag ggtgcgcgtg aagtcggcga cgcgccaggt ttgtcgacac ggctcaggac
     1741 ccgctgccac tcacagatca ccgccttctt cggcgagacg gttcaattcc ctggccagta
     1801 gggcgaggcg ggcctctttt accaggggca cttccctgcg gcggcgacgg acgttgccaa
     1861 ggtctatgtc tacggcgatc aaggcctcct cgtggagcgg cccttccgcc acgaccgtcc
     1921 cccaggggtc gacgacgtgc gagtagcccc agaagaccag gttgtcctgt ttaccgacgc
     1981 gattgacgaa gacgacgtag cactcgagca tgcgggagta gaagcgggtg atcccacgcc
     2041 agtagcccct tgtgtccaac aactcggggt aagaagggta ggagccgctg tctgctggga
     2101 ttatgagcac ctgggcgccg tcctgaactg cgatgaacgg caggaacgtc tgccaggcgt
     2161 cgttgcagat cagcatggcc atgcggccta acctggtgtc gaaggcgcgc atgctctgtc
     2221 ccgggttgta gtgttttctc tcttcgtaga tcctgtagtt gggcaagtag agcttgcggt
     2281 ggaggtgtcg aattgagcct ccttcgagat aggccgcgct gttgtaggtc tggaagcgcg
     2341 gcccctcctc gcaaaacccc acgaccacgg ccatctctcc cgccgcctcg gccagagaga
     2401 cgatgggctt gctgtgcgcc tcgatgggga catcgtcact gagctctccg agagcatagc
     2461 cggacaggct cagctcaggg aagac
//
LOCUS       DATMJA010000404         4944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_172393, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000404 DATMJA010000000
VERSION     DATMJA010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172393"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VNT12_09615"
     CDS             <1..887
                     /locus_tag="VNT12_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI46415.1"
                     /translation="EAGEHAEDMTSEPEGVRFEDAPAVGGLWATPRSWDGESAIMYLY
                     GGDYVISSPHSRRKLAGHLANAAGSRALVPKYHLAPERPFPSAVDDAAADYRWLLREG
                     YRPERIVISGDSSAGGLTVAAMLKLREDGSPLPAGGVPISPWVDLACTGETLETNAAA
                     DLTATKASLLRMAGQYLDGSDPRNPLASPLYADLSGLPPLLVVVGGAEALLDDSVRLA
                     RSAGMAGVDVTLYIGAGMQHIFPVYCGVIPESDAAVAMIGAWIRSRTSQESEQATHNG
                     KEDHVPAPGQDSEGASPI"
     gene            complement(930..1286)
                     /locus_tag="VNT12_09620"
     CDS             complement(930..1286)
                     /locus_tag="VNT12_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46416.1"
                     /translation="MPATVRIKPELITADRLRIEMFGLEDEDIENTIRMKGWAWVLAR
                     RGWVYAGEPDFIFRQIREVVIALPDITFEPEAVEESVKTVLEKARTEEEREEGRLLLH
                     QAFEKTGQLAEAEGFL"
     gene            complement(1321..2556)
                     /gene="hutI"
                     /locus_tag="VNT12_09625"
     CDS             complement(1321..2556)
                     /gene="hutI"
                     /locus_tag="VNT12_09625"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HWI46417.1"
                     /translation="MAEATLLIHDLAAAVTSAGSGPLRGREFGAVRIHSPASIAIAGE
                     RIAAVGPPAEILRELPSGEDYKSVDGRGKVALPGLIDCHTHPAFLGDRAAEFELRSSG
                     AAYEEIHASGGGIRSTVAATRAGSEGDLRSSVERHLGWMLEHGTTTAEAKSGYGLDRE
                     TELKSLRAIHDAGHSSPVDVCPTFLGAHTVPSEFESAAEYVEFVIYEVLPEAAPLARS
                     ADVFLERGSFEVPEARRYLVACQEYGLALRLHADQFSEGGAIPLALELGARSVDHLEA
                     TQHEAAILLAKSDVAAVLLPACGLFLDLPPPPARLLADEGAILALATDFNPGSSFCES
                     LPLVMNLACNRLGLSTAEALCACTANAAHVLGAEDVGRLAPGYKADVLLLDAPDWRHI
                     AYHLGGDRFTARIKSGRVL"
     gene            complement(2557..3387)
                     /locus_tag="VNT12_09630"
     CDS             complement(2557..3387)
                     /locus_tag="VNT12_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HWI46418.1"
                     /translation="MCRWLAYSGSPVLIEELLYRPEHSLIDQSLHSRLGVETTNGDGF
                     GLGWYVPHTETSAVFHSIEPAWNDRNLREVASHVESPLFLAHIRASTGTAVQQTNCHP
                     FRYGRWLWVHNGLVRDFHRIKRDLALAVDDSLYAAIEGSTDSEMMFYLALTFGLEDDP
                     PRAVERMVGHVEEVGRRHGTEHPIQMTIGTTDGNSVWGFRYSSEGKSRTLYYSTDMRT
                     VRELYPERPRLQDLSDDTRIIVSEPLVDLPGAWNEVPESSYGVVREGDDEMRPFRPVA
                     "
     gene            complement(3573..3854)
                     /locus_tag="VNT12_09635"
     CDS             complement(3573..3854)
                     /locus_tag="VNT12_09635"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWI46419.1"
                     /translation="MGLMAARAANRKQTRQTYRTMARMERRRSYVQGRMGMRQDFGRQ
                     EQPDEAAAPATAPSEPEYVGELERLSQLRDQGIISNEDFEAKKKQILGI"
     gene            complement(3903..4436)
                     /locus_tag="VNT12_09640"
     CDS             complement(3903..4436)
                     /locus_tag="VNT12_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46420.1"
                     /translation="MTEKIGPVQLVAIGFPPDAEFEGRIMAELERLESHETIRILDLL
                     FVGKDAETGDLLALDYQGEDLGAIVGALLGFEFEDDGELEGGSTEGTEGHAFGLSQSE
                     IHDMAASLRPDSSAGFVLIEHVWARDLKEAIRDAGGFPLGEGFLTPEALAEVAIELVA
                     MSEAIEELEAEEAGAGT"
     gene            complement(4522..4809)
                     /locus_tag="VNT12_09645"
     CDS             complement(4522..4809)
                     /locus_tag="VNT12_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46421.1"
                     /translation="MGCLFALFGGLFPRLALLILWIARPALVAAAFNTFLLPLLGIIF
                     LPFATLIYVIVYTPSVGVIGFDWFWVILAVLLDISHWTASYTQRNQIPGVR"
BASE COUNT          850 a         1603 c         1557 g          934 t
ORIGIN      
        1 gagaggcggg cgagcatgct gaggacatga cctcggagcc cgagggcgta cgtttcgagg
       61 acgcccccgc agtgggaggt ctttgggcta cgccacgctc ctgggacggc gagtcggcga
      121 tcatgtacct ttatggcgga gactacgtga tctcctcgcc ccactctcgg cgcaagctcg
      181 ccggccatct cgccaacgcc gcagggtccc gcgccctggt cccaaagtac catctcgccc
      241 ccgagcgccc tttcccttcg gccgtcgacg acgccgcggc cgactaccgg tggctgctca
      301 gggaggggta tcgacccgag cgaatcgtca tctccgggga ttcgagcgcc ggaggcctca
      361 cggtagccgc gatgctcaag ctgcgcgagg atggctcacc acttcctgcg ggtggtgttc
      421 ccatatcgcc gtgggtagac ctggcctgta ccggagagac gctcgagact aacgccgctg
      481 cggatcttac agcgaccaag gccagccttt tgaggatggc gggccagtat ctcgacggct
      541 ccgacccgcg caacccgctt gcctcacccc tttatgccga cctctccggc ttaccgcctc
      601 tcctggtggt cgtggggggc gcggaagcac tgctcgatga ctcggtgcgc ctcgcccgct
      661 cggctggcat ggcgggcgtt gatgtgacgc tatacatcgg ggccgggatg cagcacatct
      721 tcccggtcta ctgcggtgtg atccccgaat ccgatgcggc cgtagcgatg atcggggcct
      781 ggatacgctc ccgtacgtcg caagagagcg aacaagcgac ccataatggg aaagaggacc
      841 acgtgccagc gcccggacag gattctgaag gagcaagccc tatatagacc tatacgccaa
      901 cccccgacaa tggcgttctt gtcaggcgtc taaaggaacc cttcggcctc ggcgagctgg
      961 ccggtcttct cgaaggcctg gtgcaggaga aggcgtccct cttcgcgttc ctcctccgta
     1021 cgggccttct ccagtacggt ctttacagac tcctcgacgg cttcgggctc gaaagtgata
     1081 tccggcaggg cgattacaac ctccctgatc tgacggaaga taaagtctgg ttcgcccgcg
     1141 tatacccacc cgcgccgggc gaggacccag gcccaacctt tcatgcggat ggtgttctct
     1201 atgtcctcgt cctcgagccc gaacatctcg atcctgagcc tgtcggccgt tattagctcc
     1261 ggctttatgc gtactgtcgc tggcatgtag acagtttaac agcgacgctt cggctgaccg
     1321 tcaaagaacc cgccccgact ttatccgagc cgtgaacctg tccccaccga gatggtaggc
     1381 gatgtggcgc cagtctgggg cgtcgaggag gagcacatca gccttgtagc ctggcgcgag
     1441 cctgccgaca tcttcggcgc cgagcacgtg cgccgcgttg gccgtgcagg cacagagggc
     1501 ttctgctgta gagagcccga ggcggttgca cgccaggttc atcaccagcg gcagcgactc
     1561 gcagaaggag cttccgggat tgaagtccgt ggcgagcgcc aagatcgctc cctcgtcggc
     1621 caggaggcgg gccggagggg ggggcaggtc gaggaagagc ccgcaagcgg ggaggagcac
     1681 ggccgcaacg tcgctcttcg ccagcaggat ggccgcttca tgctgcgtag cttccaggtg
     1741 gtctacgcta cgggcgccaa gctcgagggc cagcgggatc gcacccccct cggagaactg
     1801 gtccgcatgc aagcggagcg cgagcccgta ctcctgacac gccacgaggt agcgtctcgc
     1861 ctcaggcacc tcgaaagaac cacgctccag gaagacgtcg gcggatctgg ccaggggcgc
     1921 tgcttctggc agcacttcat agatcacgaa ctccacgtac tctgccgcac tttcgaactc
     1981 ggacgggaca gtgtgtgccc ccaggaaggt aggacatacg tccacggggc tgctatgtcc
     2041 cgcgtcgtgg atcgcacgca gagacttcag ctccgtctcg cggtccaggc cgtagcctga
     2101 cttcgcctct gcggtcgtcg tcccgtgctc cagcatccag ccgaggtgac gctccacgga
     2161 tgagcgaaga tccccctcac taccagcccg cgtagccgcc accgtcgagc ggataccgcc
     2221 accggacgcg tggatctcct cgtaggcagc tcctgaggag cgcaactcga actccgcagc
     2281 gcggtctccc aggaaggccg ggtgcgtatg gcagtctatg aggcctggaa gcgctacctt
     2341 cccacgcccg tccacgctct tgtagtcctc gccggatgga agctcccgta agatctccgc
     2401 gggcgggccg acggcggcga tgcgctcacc cgcgatagct atcgaggcgg gggagtggat
     2461 acgcacggct ccgaattccc tgccccggag aggtcccgac cctgccgagg tgacggcggc
     2521 ggcgaggtcg tggatcagta acgtggcctc agccattcat gcgacaggcc ggaacggacg
     2581 catctcgtca tctccctctc ttacgacgcc gtagctcgat tcaggtacct cgttccacgc
     2641 cccgggcagg tccacgagcg gctcggagac gatgatccgc gtatcgtcgg agagatcctg
     2701 caggcgcggg cgctcagggt agagctcccg caccgtacgc atgtcggtgg agtagtacag
     2761 cgtgcgtgac ttgccttcgc tggagtatct gaacccccac acgctgttcc cgtccgtggt
     2821 cccgatcgtc atctggatgg ggtgctcagt gccgtggcgg cgtcccacct cctcgacgtg
     2881 ccccaccatc ctctccaccg cccgcggggg atcgtcctcc aagccgaacg tgagggccag
     2941 gtagaacatc atctccgaat cggtggagcc ctcgattgcg gcgtaaagcg agtcgtccac
     3001 ggcgagcgcc agatcgcgtt ttatgcgatg gaagtccctg acgaggccgt tgtggaccca
     3061 cagccacctg ccgtaacgga aagggtggca gttggtctgc tgaaccgccg tgcctgtaga
     3121 ggctctgatg tgggccaaga acagcggtga ttcgacgtgt gaggcgacct ctctgaggtt
     3181 gcggtcgttc caggccggct cgatgctgtg gaagacggcg gaggtctcgg tatgggggac
     3241 gtaccaaccg aggccgaagc cgtctccgtt agtggtctcg acgccgaggc gggagtggag
     3301 ggactggtcg atgagggagt gctcgggcct gtagagcagc tcctcgatga ggacgggtga
     3361 gcccgagtag gctagccagc ggcacattgt ttgctccttt ccgttcgcag gtcgacttct
     3421 gctggcctat taccttacgg ctaatgggcc aggggtgctg gcattgatga cgaactcgcc
     3481 gagttggcac cagaaagcga cccaccgcgg cccgggacga tgcccatcct tccacggtgg
     3541 gtctctcctg gcgtcactcg acgcgcgttc tttcagatac ccaggatctg cttcttcttg
     3601 gcctcgaagt cctcgttgct gatgatgccc tgatcccgaa gctgggagag ccgttcgagc
     3661 tcacccacgt attcgggctc ggacggggca gtggcaggtg ccgctgcttc gtccggctgt
     3721 tcctggcggc caaagtcctg tctcattccc atcctgccct ggacgtacga gcggcgtcgc
     3781 tccatgcgcg ccatcgtccg gtaggtctgc cgcgtctgct tccggtttgc ggctcgtgct
     3841 gccataagtc ccatcaatct tctccttccc tgcgtccgtt ggttcggctc gctcgatctt
     3901 ccctacgtcc cggcgcccgc ctcctcggcc tccagttcct cgatggcctc ggacatcgcg
     3961 acgagctcga tagcgacctc ggcgagggcc tctggagtga ggaacccctc ccctagcggg
     4021 aagccgcccg cgtcgcggat ggcttccttc agatcgcgcg cccacacgtg ctcgatgagg
     4081 acgaatccgg cggagctgtc cgggcgcagc gaggcggcca tgtcgtgtat ctcgctttgt
     4141 gagagcccga aggcgtggcc ttcggtacct tcggtcgatc cgccctctag ctcgccgtcg
     4201 tcctcgaact cgaagccaag cagcgctccg acgatcgcgc ccagatcttc gccctggtag
     4261 tcgagggcga gaagatcccc cgtctcggca tccttgccta cgaacagaag gtccaggatg
     4321 cgaatggtct cgtggctctc cagcctttca agctcggcca tgatcctgcc ctcgaactcc
     4381 gcatccgggg ggaagccgat cgccacgagc tgaaccggcc ctattttctc cgtcatcgtt
     4441 cacctccgtc tgttgtccgc cgagtcacgc tgttcctctc gcctccatac gcgggtagat
     4501 ccctgccgtg attcctcgcg tttaccgtac cccggggatc tggttgcgtt gggtgtagct
     4561 ggccgtccag tggctgatgt cgagcaggac ggcgagaatc acccagaacc aatcgaagcc
     4621 tatgacgccg acgctggggg tgtagacgat aacgtagatc agggtcgcaa acgggaggaa
     4681 gatgatgccc agcagcggca ggagaaaggt gttgaacgcc gcagcgacga gggccggtct
     4741 ggcgatccag agtatgagca gcgcgaggcg cgggaagagg cctccgaata gcgcgaatag
     4801 acaacccatg ctgtttacct cctatccggt gtttgctcct ggtctgcaag acgtctctcg
     4861 agaataagcc caagcaagat ggtgggtcat cccccaggga cgagtatccc ctaggggaat
     4921 tccacccatg aaggtcgatg ggca
//
LOCUS       DATMJA010000405         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_54226, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000405 DATMJA010000000
VERSION     DATMJA010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54226"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VNT12_09650"
     CDS             complement(<1..872)
                     /locus_tag="VNT12_09650"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWI46422.1"
                     /translation="MKAQTLLGVTGSGKTHTMARVIEEAGSPALVIAHNKTLAAQLAS
                     EFAEFFPNNAVEYFVSYYDYYQPEAYVPRTDTFIEKDAQINEDIDRLRHSATSSLFTR
                     RDVIVVASVSAIYGLGSPEEYRSKMVLLEQGGFYDLDDVLRDLVKIQYTRNDFQLNRG
                     NFRVRGDVLEVHPAYQDTIYRISFFGDEVENMIEADPLTGEILREHEILTVYPASHFV
                     TGEDRLATAVKNIETELEERLGELEGEGKVLEAYRLKQRTQYDLEMMRELGYTSGIEN
                     YSRHLDGRLPGSTP"
     gene            complement(955..1485)
                     /locus_tag="VNT12_09655"
     CDS             complement(955..1485)
                     /locus_tag="VNT12_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWI46423.1"
                     /translation="MVIGILTILPFALRLPDAVMRTVYPLKYTATIREVSEENDLDPT
                     FVAGVIYTESRFRPSVRSHSKAYGLMQLLPQSAHFIQRKSGIKGNYRDPEVNIKLGTW
                     FLGYLNRRYKGDERLMLAAYNSGEGSVDAWVSDKGFDIANDIPYKETRHYVNNVLEAE
                     QIYKELYGRNLNRDSE"
     gene            complement(1544..2161)
                     /gene="coaE"
                     /locus_tag="VNT12_09660"
     CDS             complement(1544..2161)
                     /gene="coaE"
                     /locus_tag="VNT12_09660"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWI46424.1"
                     /translation="MEGPVTVAVTGPFASGKSMLVRFMGELPGTETASADEIVHHLLK
                     NDRRTISRIIERFGEGVGGPDGIDRRALGREVFGDAGALRDLEEILHPLVRSETDRRI
                     EASMAELFFVEIPLLFETGRGGDFDFTVAVTVPEERRRVWAEERGLDEAALRAVEARQ
                     LSGEEKARRADLVIQNDGDLDRLREQAYELGRKISRRRGSNANPR"
     gene            complement(2191..>2629)
                     /locus_tag="VNT12_09665"
     CDS             complement(2191..>2629)
                     /locus_tag="VNT12_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWI46425.1"
                     /translation="RISLGLKQTRKDPWQEIVERVKVGEQIHGRVTKLVSFGAFVEVA
                     DGVEGLIHISELADHHVETPDEIVRSGDEVDARIIDVDARRRRLSLSLRPKREEREER
                     PRREERPRREPEQRGPSDRRSSEGGGLRSGAFDALADLDLEER"
BASE COUNT          444 a          837 c          782 g          566 t
ORIGIN      
        1 tacggcgtgg agccaggcag acgcccgtcg agatggcggg agtagttctc gatacccgag
       61 gtgtagccga gctcgcgcat catttccagg tcgtactggg tgcgctgctt gaggcggtat
      121 gcttccagaa ccttgccctc gccttccagt tcgcccagcc gttcctcgag ctccgtctcg
      181 atgttcttga cggcggtcgc gagcctgtct tcgcccgtga cgaagtggga cgcggggtag
      241 acggtcagga tctcgtgctc gcgtaggatc tcacccgtca aggggtcggc ctcgatcatg
      301 ttctcgacct cgtccccgaa gaaggagatg cggtagatag tatcctgata agcgggatgc
      361 acctccagga cgtcgccacg taccctgaag ttgcccctgt tgagctggaa gtcgttcctg
      421 gtgtactgga tcttgacgag gtccctcaaa acgtcgtcga ggtcatagaa gccgccttgc
      481 tccagtagaa ccatcttcga acggtactcc tccggactgc cgagaccgta aatggcggag
      541 acgctcgcga cgactatgac atcgcggcgg gtgaacaggg aactcgttgc cgagtgacgc
      601 agcctatcta tgtcctcgtt gatctgggcg tccttctcga taaatgtgtc cgtgcgcggg
      661 acgtaagcct cgggctggta gtagtcgtag tagctgacga agtactcgac ggcgttgttg
      721 gggaagaact cggcgaactc gctggctagc tgcgcggcga gcgtcttgtt gtgcgcgatg
      781 acgagcgccg gactaccagc ctcctctata acacgagcca tagtatgggt cttcccagat
      841 ccggtaacgc cgagcaaggt ctgtgccttc atgcccgcgt tgaggcccga agcgagctgc
      901 tctatggccc gcggctggtc gccggtcggc tggtattcgc tgtttacctg gaagctactc
      961 tgagtctcga tttaagttcc tcccgtaaag ttccttgtag atctgctcgg cctcgagcac
     1021 gttgttcacg taatggcggg tctctttgta gggtatgtcg ttcgcgatat cgaagccttt
     1081 gtcggagacc caggcgtcaa cggatccctc ccctgaatta tacgccgcga gcatgagtct
     1141 ctcatcacct ttataccgtc tattgaggta accgaggaac caggttccca attttatgtt
     1201 gacctccggg tcgcggtagt tgccctttat gccgctcttg cgctggatga agtgcgccga
     1261 ttggggaagt agctgcatga gcccgtaggc cttactgtgg gatctgacac ttggcctgaa
     1321 gcggctctct gtgtagatca caccagccac gaacgtggga tcgaggtcgt tctcctcgct
     1381 gacctccctg atggtcgctg tgtacttcag aggatacacc gtgcgcatca cggcatcggg
     1441 tagtctgagg gcgaaaggca ggatagtaag gatcccaata accaccagca acacgagaat
     1501 cccgcgccgg cggcgggccc gcgacctgcc gtacctgcgt ctcttaccgt gggttggcgt
     1561 tggatcctct cctccgcgag atcttgcgcc cgagttcgta cgcctgctcc ctaagtctat
     1621 ccaggtctcc atcattttgt atcaccaggt cggcccgcct ggccttctcc tcacccgaga
     1681 gctgccgtgc ctccacggcc cgtagcgccg cctcgtccaa gccccgctcc tcagcccaca
     1741 cgcggcgcct ctcttccggc accgtgacgg ccaccgtaaa gtcgaagtcc ccaccccggc
     1801 ctgtctcgaa cagcagcggt atctcgacga agaagagctc cgccatagaa gcctcgatac
     1861 gcctgtcggt ctcgctacgt accagaggat gcagtatctc ttcgaggtcc cgcagtgccc
     1921 cggcgtcacc gaatacctcg cggccgagag ccctgcggtc gatgccgtcc ggtcctccga
     1981 caccttcccc gaaacgttcg atgatcctgg atatcgtccg gcggtcgttc ttcaggaggt
     2041 ggtgcacgat ctcatcagcc gaggccgtct ccgtgcccgg cagttcaccc atgaagcgga
     2101 ccagcatact cttgcccgac gcgaaagggc cggtgacggc tacggtcacc ggcccttcca
     2161 cggaggaccc tctctgtaaa gagtccttgt ctatctctcc tcgaggtcca ggtcggccag
     2221 cgcgtcgaag gcgccggagc ggaggccgcc cccctcgctg cttctgcggt ccgaggggcc
     2281 gcgctgctcg ggctcgcggc gcgggcgctc ctcgcggcgg ggacgctctt cgcgttcctc
     2341 gcgcttcggg cgcaggctca aggagagcct gcgtcggcgg gcgtcgacgt cgatgatgcg
     2401 ggcgtcgacc tcgtcgccgc tgcgcacgat ctcgtctggc gtctccacgt gatggtcggc
     2461 cagctcggag atgtggatca aaccctccac cccgtctgcg acctccacga aggccccgaa
     2521 tgaaacgagc ttggtaaccc taccgtggat ctgctctccg accttgaccc gctcgacgat
     2581 ctcctgccag ggatctttcc tggtttgctt gaggccgagc gagatgcgc
//
LOCUS       DATMJA010000406         2482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_291238, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000406 DATMJA010000000
VERSION     DATMJA010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291238"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /gene="thrC"
                     /locus_tag="VNT12_09670"
     CDS             complement(<1..784)
                     /gene="thrC"
                     /locus_tag="VNT12_09670"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWI46426.1"
                     /translation="MNVQARVSRQGVIRRYRRWLPEIPDDAIATIGEGETPLIEATRI
                     SDRVGARLFLKFEGMNPTASFKDRGMTVAMSRAAATGSRACVCASTGNTAASAAAYAA
                     RASMECFVVVPAGKIALGKAVQVLAHGARIVQIEGNFDAALKLSKRIAEELGDVTLVN
                     SVNPDRIEGQKTAAFEVAEALGDPPDAVALPVGNAGNITSYWKGFREWRGAGHAGSVP
                     RMLGFQAEDASPLVAGHDFESPETVASAIRIGSPASKEGALSA"
     gene            1033..2103
                     /gene="rfbB"
                     /locus_tag="VNT12_09675"
     CDS             1033..2103
                     /gene="rfbB"
                     /locus_tag="VNT12_09675"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWI46427.1"
                     /translation="MQREKQTILVTGGAGFIGGNFVLSTVGDYKVVDLDALTYAGNPD
                     TLASVSDNPDHVFVRGRIGDRELVDRLLTAHRPSAVVNFAAETHVDRSIDGPRAFVQT
                     NVVETHELLEAVLSYWSVLEGEDHDAFRFLHISTDEVYGTLGPEGYFTEESPFRPNSP
                     YAASKASSDHLVRAYHHTYGLPTLTTNCSNNYGPYHYPEKLIPLMILTALRGEPLPVY
                     GDGQQIRDWLYVEDHCRAILAVLQRGRLGETYNVGGHNERTNLEVIKTICALLDELVP
                     GSPHESLITFVADRPGHDRRYAIDARKIERELGWRPEETMETGMEKTVHWYLENRGWC
                     EAVLSRGYGGERLGLGRRGGLV"
     gene            2154..>2482
                     /locus_tag="VNT12_09680"
     CDS             2154..>2482
                     /locus_tag="VNT12_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWI46428.1"
                     /translation="MAISKQLLPVYDKPMIYYPLTTLMLAGIRDILVISTPEDLSRFE
                     DLLGDGSQWGVDFSYAVQPRPEGLGQAFVIGREFVGSESVSLVLGDNIFFGHGFSELL
                     ERSVGRQ"
BASE COUNT          479 a          813 c          752 g          438 t
ORIGIN      
        1 cggccgagag tgccccctcc ttgctcgcgg gtgaacctat cctgatggcg ctcgcgaccg
       61 tctcgggact ctcgaagtcg tggcccgcta ccagcgggga ggcgtcttcg gcctggaagc
      121 cgagcatgcg ggggaccgag ccagcgtgcc ccgccccgcg ccactcacgg aagcccttcc
      181 agtacgaggt gatgttgccg gcgttcccca ctggcagagc caccgcgtcg ggcggatctc
      241 cgagggcttc cgcaacctcg aaagccgcgg tcttctggcc ctctatgcgg tcggggttca
      301 ccgagttgac cagggtgacg tcgccgagct cctcggcgat cctcttggag agcttcaatg
      361 ccgcgtcgaa gttgccctcg atctggacta tccgcgcccc gtgcgcgagg acctgtacag
      421 ccttgcccaa cgcgatcttg cctgccggca cgacgacaaa acactccatg ctcgcacgcg
      481 ccgcgtaggc cgctgccgag gccgctgtat tccccgtcga ggcgcagacg caggcgcggc
      541 tccccgttgc ggctgccctg ctcatggcca ccgtcatgcc gcggtccttg aacgaggccg
      601 tcgggttcat cccctcgaac ttcaggaaca ggcgggcccc gacgcgatcc gagatcctcg
      661 tggcctcgat caggggcgtc tccccctcgc ctatggtggc tatggcatca tccggaatct
      721 ctggcagcca cctgcggtac ctccggataa caccctgtct cgacacccgc gcctgaacgt
      781 tcaatacaag ctagcctttc attggagatc gggccgattc gactttacgg acccggaatc
      841 tagccgcgaa cagtagtgta aaagccacag cgtaagaaaa gtatcacgca ccggcgctgg
      901 gttgcaagaa cttggcgacc ccacgacccg tctagtaggc tacaggctgt aaagccatca
      961 gccgtcagcg atcagccaaa aagaatatga aatctgatgg ccgaccgctg acaactaaaa
     1021 cagaggagcg cattgcagcg agagaaacaa accatcctcg tcaccggcgg cgccggcttt
     1081 atagggggca acttcgtcct gagtacggtc ggagactaca aggtggtcga tctcgacgcc
     1141 ctgacctacg ccggcaaccc tgacaccctg gcgtcggtct cggacaaccc agaccacgtc
     1201 ttcgtacggg ggagaatcgg ggatagggag ctggtggacc gcctgctcac tgcacatcgt
     1261 ccctcggccg tcgtcaactt cgccgccgag acgcacgtcg accgctccat cgacggtccg
     1321 agggcctttg tacagaccaa cgtggtcgag acccacgaac tcctcgaggc tgtgctctcc
     1381 tactggagcg tcctcgaagg ggaagatcac gacgccttcc gctttctgca catctccacg
     1441 gacgaggtgt acgggacgct ggggcctgag ggctacttta ccgaggagag ccctttcagg
     1501 cccaactccc cctacgccgc ctccaaggcg tcatccgacc atctcgtgcg ggcctaccac
     1561 cacacctacg ggctccccac gctaacgacc aactgctcca acaactacgg gccctaccac
     1621 taccccgaga agctcatacc gctcatgatc ctgaccgccc tgcgtggaga acccttgccc
     1681 gtctacggag atggtcagca gatcagggac tggctctacg tagaggacca ctgcagggct
     1741 atactcgctg tactccagcg cggccgtctt ggggagacgt acaacgtcgg cggccacaac
     1801 gagcggacga acctcgaggt catcaagacc atctgcgccc tgctcgacga gctagttcct
     1861 ggatcgccgc acgagtcgct catcaccttc gtcgcggaca ggccgggcca cgacaggcgt
     1921 tatgcaatag acgcgaggaa gatagaacgc gagctcggct ggagacccga ggagacgatg
     1981 gagacgggca tggagaagac cgtgcactgg tacctggaga accgcgggtg gtgcgaagcc
     2041 gtgctctcgc ggggctacgg gggggagcgg ttagggttgg gcagacgcgg agggctggtg
     2101 tgaaggggat cgtgctcgcc ggaggatcag gtacgcggct gtatccgctc acgatggcga
     2161 taagcaagca actccttccc gtctacgaca agccgatgat ctactaccct ttgaccacgt
     2221 tgatgctcgc gggcatcagg gacatactgg tgatctccac ccccgaagac ctttcccgct
     2281 tcgaggatct gctcggcgac ggatcgcagt ggggtgtaga tttcagctac gccgtgcagc
     2341 ccaggccaga aggcctgggc caggctttcg tcatcgggcg ggagtttgtg gggtccgaga
     2401 gcgtctcgct cgtcctcggg gacaacatct tcttcgggca cggtttctcc gagctacttg
     2461 agcggtccgt aggcaggcag ag
//
LOCUS       DATMJA010000407         3969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_705464, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000407 DATMJA010000000
VERSION     DATMJA010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705464"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VNT12_09685"
     CDS             <1..568
                     /locus_tag="VNT12_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWI46429.1"
                     /translation="LGFGLVLLARAVRADPDILRCARPVCERLSERTRETVTIAVLEG
                     DDAVVIHQSISRASAVGVDWTGRHTPLHATAAGKIFLAYMPEDQLLRILEGPLERFTK
                     NTIVDPASLKDNTGKVRDEGYGYTVEELETGLNAVGAPIRGADGAVVGAVSVSGPAFR
                     LRPDAFPEIGELSRRAAAEISRCLGFQG"
     gene            591..749
                     /locus_tag="VNT12_09690"
     CDS             591..749
                     /locus_tag="VNT12_09690"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HWI46430.1"
                     /translation="MTFERSIRVTFEHGHANKRSDDYSSVAYWYQSEPHSHFSILTVE
                     QRVDRASN"
     gene            complement(775..2061)
                     /locus_tag="VNT12_09695"
     CDS             complement(775..2061)
                     /locus_tag="VNT12_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWI46431.1"
                     /translation="MQTENPVYEEIQEILRAVELPSVALVGQRIDSPPALANIREGVR
                     EALDSVELPSGSVAIGVGSRGVARIGEVVAALVEALKDSGAEPFIVPAMGSHGASTAE
                     GQAQVLAHLGVSEEQVGCPVRATMESVRIGETPSGVAVYMDRNAYEADSVIVVNRVKP
                     HTAFRGAVESGPTKMLAIGLGKQRGAHSIHSAGWENIHQTIPEAARVAVESGKVAFGL
                     ATVENAEEEPCKIVAIPAGELEEAEAPLLEEAKRNLARLPFDEIDVLVVDEIGKNISG
                     DGADPNVTGRYPTSAASGGPSVQRMVYLGLTEETGGNANGLGMADVVTERLASSVDRP
                     STYMNALTSTTPAPVKTPMVMPTDEMALAAALTMCAGVDPAEARLVRIENTLKLGRIW
                     VSETLLREAENNERLEILEEPQPMRFEGGEIIPRSA"
     gene            complement(2095..2505)
                     /locus_tag="VNT12_09700"
     CDS             complement(2095..2505)
                     /locus_tag="VNT12_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWI46432.1"
                     /translation="MNGDPREGGCFCGRVRYRVTGEPVGSAVCHCVSCRRTSGAQSVA
                     WLTFPHRKFSFVHGEPADYRSSDEVSRTFCQSCGTSLTYRHDEEPDSIDVTTASLDRP
                     EEFPPTRHVWLEDKLAWEGVDDGLPRFEQGGPPD"
     gene            2649..3386
                     /locus_tag="VNT12_09705"
     CDS             2649..3386
                     /locus_tag="VNT12_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWI46433.1"
                     /translation="MRAALFIPCYVDQINPEVGVSVVRVLRRLGVEVTYPEGQTCCGQ
                     PAFNSGFFDEARAIGRRFLDVFERERFDYIVCPSGSCTTMVSHYYPFVFENQALPDDR
                     ERAEALGHRVREFSDFVVNVLGVKDLGASHAGRAVFHTGCHQRRELGVLNEPRDLLKG
                     VSGLELLEWENEELCCGFGGMFSVKMPDVSTAMADEKIAALEKSGADTVISCDSSCLM
                     HLGGRLRRTGHDTRVLHLAQILDSDGR"
     gene            3392..>3969
                     /locus_tag="VNT12_09710"
     CDS             3392..>3969
                     /locus_tag="VNT12_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HWI46434.1"
                     /translation="METSKPARRRDTEDRRREGLESKVEGFNERGRKAVRDRKLHDSI
                     SLFTNKTLIGRNAQLRAHPDAPELRERAYRAKQETMANLDYYLSQMADAIEERGGNVF
                     FADDGEDVVEYIGNLARRRGAKILTKSKSMATEEIELNHRLEEDYADLGLEIVETDLG
                     EWIAQLAGDTPSHIVGPIVHMNMERITEILSGV"
BASE COUNT          724 a         1244 c         1249 g          752 t
ORIGIN      
        1 gctgggcttc gggctggtgc tactcgcgag agcggtgagg gccgaccctg acatccttcg
       61 ctgcgccagg ccggtctgcg agcgtttgag cgagcgtacc agggagactg tcaccatcgc
      121 tgtcctggaa ggtgatgacg cagttgtcat tcaccagagc atttcgcggg cgtccgccgt
      181 cggcgtcgac tggacgggac gtcacacgcc gttgcacgcc acggccgctg ggaagatctt
      241 tctggcgtac atgccagaag atcaactcct gcgtatcctg gaaggaccgc tggagcgctt
      301 taccaagaac acaatcgttg acccggcgag cctgaaagac aatacaggta aggttcggga
      361 cgaagggtat ggctataccg tcgaggaact ggagacgggg ctgaacgctg tgggggcgcc
      421 gattcgcggc gccgacggcg cggtagtggg tgcggtgagc gtgtctggcc cggccttccg
      481 tctgagaccg gatgcgttcc ccgagatcgg cgaactatcc agacgggccg ccgccgagat
      541 ctcccggtgc ctgggattcc agggctgaac cgagctgcgg aggctctccc gtgacgttcg
      601 aacgctccat cagggtcacc ttcgagcacg gccacgccaa caagcgctca gacgactact
      661 catcggtcgc ctactggtac cagtccgaac cgcacagcca cttctctatc ctcaccgtcg
      721 aacagcgcgt ggaccgtgca agtaactgaa acctgtaacg tgatgcctgg agtgctatgc
      781 actccgggga atgatctcgc ctccttcgaa gcgcattggt tggggttctt ccaggatctc
      841 cagccgctcg ttgttctcgg cttcgcggag cagtgtctcc gacacccaga tccttcctag
      901 cttgagtgtg ttctctatgc ggacgagcct ggcctcggcg gggtcgacgc cggcgcacat
      961 ggtcagggca gcggcgagag ccatctcatc cgttggcatg accatgggcg tctttaccgg
     1021 tgcaggcgtg gtcgaggtga gggcgttcat gtaggtggag ggacggtcca ccgagctggc
     1081 gaggcgctcg gttacgacat ctgccatgcc gagaccgttt gcattgcctc cggtctcctc
     1141 tgtcaggccg aggtagacca tacgctgcac agaagggccg cctgaggcgg cactcgtcgg
     1201 gtatcggcct gtgacgttag gatcggcgcc atcgccagag atgttcttcc cgatctcatc
     1261 taccacgaga acgtcgatct catcgaacgg cagccgcgcg aggttgcgct tcgcttcttc
     1321 gaggagcggc gcttcagctt cctccagctc cccggcgggg atcgcgacta tcttgcatgg
     1381 ctcttcttct gcgttctcga cggtggcgag accgaaggcg accttgccgc tctcgacggc
     1441 gacgcgggcc gcttcgggaa tggtctggtg gatgttctcc cagcccgcag agtggatgga
     1501 gtgcgccccc cgctgcttgc cgagcccgat ggcgagcatc ttcgtcggcc cgctctccac
     1561 tgctcccctg aacgcggtgt gcggcttgac cctgttgacc acgatgaccg agtcggcctc
     1621 gtacgcgtta cggtccatgt agactgctac gcctgagggt gtctccccga ttctcacgct
     1681 ctccatcgtc gcccgcacgg gacacccgac ctgctcctcg ctaacgccaa ggtgcgcgag
     1741 cacttgcgcc tggccttccg cggtcgaggc gccgtggctc cccatcgcgg ggacgatgaa
     1801 aggctcggcg cctgagtctt tcagggcctc gaccagcgcc gcgactacct cccctatcct
     1861 ggcgaccccc ctgctcccga cgcctatggc cacagagcca gacggtagct ccacggagtc
     1921 cagcgcctcc cggacgccct ccctgatgtt cgcgagcgcc ggcggagaat ctatccgctg
     1981 cccgacgagc gcgacactgg ggagttcgac agcgcgcagg atctcctgta tctcctcgta
     2041 gacggggttc tctgtctgca ctgcctaacc tccgtataga ctgactttac aacgctaatc
     2101 cggcgggcca ccctgctcga accggggcag accgtcgtcg actccctccc aggcgagttt
     2161 gtcttccagc catacatggc gtgtgggagg gaactcctcg ggacggtcca ggctcgcggt
     2221 agtcacgtcg atcgagtcgg gctcctcgtc atgcctgtag gtgagcgacg tgccgcaact
     2281 ctggcagaat gtcctgctca cttcatcgga agagcggtag tcggccggct caccgtggac
     2341 gaaggagaat tttctatggg ggaaggtaag ccacgcgacc gactgcgcgc ccgacgtcct
     2401 gcggcacgag acgcagtggc agaccgcaga cccaacaggc tccccggtca ccctgtacct
     2461 cacccttccg caaaaacatc caccctcgcg gggatcgccg ttcatccctt ctccctaccc
     2521 ttctcggtac ccgcgaccat tctagcagcc cgccgtaacc tattcgccgt tccaaaccta
     2581 cgcgtgttgc ttcgctggat catccggttg ctatactggc cttggaagtc ggcggggaga
     2641 ggagtccatt gagagcagcc cttttcatac cgtgctacgt ggaccagatc aatcccgagg
     2701 ttggcgtaag cgtggtgcgc gtacttagga ggcttggggt agaggtgacc taccccgaag
     2761 gccagacctg ctgcggacaa cctgccttca actccggctt cttcgacgag gcccgtgcta
     2821 tagggcgccg cttcctcgac gtcttcgaga gagagcgctt cgactatata gtctgtcctt
     2881 ccggatcttg taccactatg gtctcccact actacccatt cgtcttcgag aatcaggctc
     2941 tgcccgacga ccgcgaacgc gcggaggctc tcggccaccg ggtcagggag ttctcggact
     3001 tcgtcgtgaa cgtgctcggc gtaaaggacc tgggcgccag ccacgctggg cgcgcagtat
     3061 tccacaccgg ctgccaccag cggcgagaac tcggggtctt gaatgagccg cgagaccttc
     3121 tgaagggcgt ctccggcctg gagcttctgg agtgggagaa cgaggaactg tgctgcgggt
     3181 tcgggggtat gttctcggtc aagatgccgg acgtttcgac cgctatggcg gatgagaaga
     3241 tagcagctct ggagaagagc ggggctgaca cggttatatc ctgtgactcg agttgtctga
     3301 tgcatctcgg aggcaggctg cgccggacgg ggcacgacac acgtgtcttg catctggccc
     3361 agatactcga ctccgacggg aggtagggca cgtggagacc agcaagcccg cgcgccggcg
     3421 cgataccgaa gaccgcaggc gggaagggct ggagagcaag gtcgaaggct tcaacgagcg
     3481 tgggcggaag gcggtcagag atcggaagct gcacgactcg atctctttgt tcaccaacaa
     3541 gaccctcatc ggacgcaacg cccagttgag agcccatccc gacgcgccgg agttgaggga
     3601 aagggcgtat cgtgccaaac aggagacgat ggcgaacctc gactactacc tttcgcagat
     3661 ggccgacgcc atcgaggagc ggggcggaaa cgtgttcttc gcagacgatg gggaggacgt
     3721 agtcgagtac atcggaaacc tggcgcggag gcgtggggcg aagatcctca ccaaatccaa
     3781 gagcatggcg accgaggaga tcgagctgaa ccaccgtctc gaagaggact acgccgacct
     3841 cggtctggag atagtggaga cggacctggg agagtggatc gcccagctcg ccggagacac
     3901 accctcgcac atcgtcggac caatcgtgca catgaacatg gagcggatca cggagatcct
     3961 ctccggcgt
//
LOCUS       DATMJA010000408         4439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_765296, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000408 DATMJA010000000
VERSION     DATMJA010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765296"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1385)
                     /locus_tag="VNT12_09715"
     CDS             complement(102..1385)
                     /locus_tag="VNT12_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HWI46435.1"
                     /translation="MVELDDGFAQTIRGAVQARREDAVSLLQELVRTRSVTGEEGAVG
                     KVVERAFSERGLDVDTWDATPEEMEPYRDHVGEQSSYENRPNVAGTRRGTGDGRSILL
                     NAHTDTVDPGDPTAWKGDPFSGNLEGDLLYGRGSCDMKGGLVTHLVALDALSDLGLRL
                     RGDVTVAATVGEENGGLGALSTVLRGYRADAAIITEPTRLALVPAQGGSLVFRLKIPG
                     RSAHAAVRDEGVSALEKFVPIFENLRELEQERNGVLSHPLYDGVRNKVPINIGVVRAG
                     NWASTVPESLVAEGRVGLIPGEEVDPFKDLVSERIATVADRDPWLREHSPELEWSGGQ
                     FAPAEVEPNAPICEAVKKAHEQSTGETPAVEGVPYGADMRLFIRFGDMPCVMYGAGDV
                     NVAHAPDEHISVTELLTATTTVACLLADWCGVDED"
     gene            1646..2488
                     /locus_tag="VNT12_09720"
     CDS             1646..2488
                     /locus_tag="VNT12_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI46436.1"
                     /translation="MTRRSRSHSLAVLVLAVAVAVTLVLLVGCSAGTDESFEGAASGE
                     TTGPIGIVGGKITVASNIAYPPFESSPKGRPRGFDIDLMNEIGKRVGFEVEYENVRFD
                     TILRGLDTDLFDAAISAMTITEDRERQIDFSDAYFNADQALLVASDSEVHSIDELAEE
                     PVGVQAGSTGQIKAEELLDGEQIGEIMPYRTIGDAFAALEDGKVEGVIYDLSAAHRKI
                     VESDREIRFVEPISTGEQYGIAFPKDSPLVDPVNRALAEIKDDGTYEKLYKKWIGMPP
                     EEIP"
     gene            2701..4278
                     /gene="murJ"
                     /locus_tag="VNT12_09725"
     CDS             2701..4278
                     /gene="murJ"
                     /locus_tag="VNT12_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWI46437.1"
                     /translation="MTSILRSILSISAATILSRATGYARWMAQAAALGAGYVADAYTA
                     SIILPSLIYELFMGGILYSIFIPVLVDRITKVGEEDARRLTNALFTLVLPLMVILSVL
                     GIVFARPLLLLVTDWQSGLSPTDAERTLDLALLFFRIFILQMFFYGISTIGTGVLQAH
                     RHFFLPTFAPVLNNLMVVASFLAYSLLVGYDQILAFYVLAIGVAAGVAVMALALVPTM
                     WRLGYRPRPQFGHPALLPTARLAGPMVILVAASVGFQLFGAYLATGFEALSELNYAFT
                     IFSLPYGVFVVAIATAIMPDLSEKHSLRDADGYRETFSFGLRTMIFVVIPSAVGMISL
                     SRPIVGLLYERGSFDAEDTETVSRLLVVFAAGLLGYSVYFFLVRAFYSRQNTKTPAAL
                     NVAIFLLYAVLAYGLSRVLGVTGVVLALSIAYAVLAILGLAATRREIGLIEGRRLLRS
                     LLKILAAGALMYVVARTGTLLLGAGSDLTERLFILVTIGGASLAVYIGVAFLLRTEEL
                     ASAFTLLRRRSAGKSAG"
BASE COUNT          755 a         1424 c         1389 g          871 t
ORIGIN      
        1 gccacgtcgc cgatgcgagg gaggaagtag agcaagtgga agaggaggtc gcgatctaac
       61 gctgccagga ggggctaggg gcgcacctag cccctcctct gctagtcctc atccacgccg
      121 caccagtcgg ccaggaggca ggcgaccgta gtggtcgcgg tcagaagctc tgtgacgctt
      181 atgtgctcgt ccggagcgtg cgccacgttc acgtcaccgg cgccgtacat cacgcagggc
      241 atgtccccga agcggatgaa cagccgcatg tcagcgccgt aggggacgcc ctcgacggcg
      301 ggcgtctcac ccgtcgactg ctcgtgggcc ttctttacgg cctcgcagat cggggcgttc
      361 ggctcgactt cggcgggtgc gaactgaccg ccggaccact ccagctccgg agaatgttcg
      421 cgcagccagg ggtcgcggtc ggcgacggtg gcgatacgtt cggacacgag atccttgaag
      481 gggtctactt cctcgcccgg gatcaggccg acgcggccct ccgcaacgag ggactcgggg
      541 acggtcgagg cccagttgcc ggcccgcacg acgcctatgt taatgggaac cttgttccgc
      601 acgccgtcgt agagggggtg gctgaggacg ccgttgcgct cctgttccag ctcgcgcagg
      661 ttctcgaaga tgggaacgaa cttctccagc gccgagacgc cctcgtcgcg gacggcggcg
      721 tgggccgagc ggcctgggat cttcagcctg aagacgagcg aaccgccctg tgcaggcacc
      781 agagcgagac gggtaggctc ggtgatgatg gcggcgtcgg cgcggtagcc gcgcaggacg
      841 gtcgagaggg cgccgagtcc cccgttctcc tcccccacag tcgccgctac ggtgacgtcg
      901 ccgcgcagcc tgagaccgag gtcggagagg gcgtcgagcg cgacgaggtg ggttacgagc
      961 ccgcccttca tgtcgcagga gccacgtccg tagagcaggt cgccctccag gttgcccgag
     1021 aatggatccc ctttccacgc ggtcgggtct cccgggtcca cggtgtcggt gtgggcgttg
     1081 agcaggatgg agcgtccgtc ccctgtccct ctccttgtgc cggcgacgtt cggtcgattc
     1141 tcgtaagagc tctgttcccc gacgtgatcg cggtacggtt ccatctcctc gggcgtcgca
     1201 tcccacgtgt ccacgtcgag gccacgctcc gaaaaggcgc gctcgacgac cttccccaca
     1261 gcgccctcct cgcccgtcac agaacgggtg cgcacgagct cctgcaacag gctcaccgca
     1321 tcctcgcgcc tggcctgtac ggcgccgcgt atggtctggg cgaatccgtc gtcgagctca
     1381 accatgagtc gcgtactccc tttcagttcc aggtaacgcg gcaccgtagc atgaggctcc
     1441 tttccaggca acgcccggcc tgcttgacca gatgccgatg cgtaattagt attgtcgttc
     1501 tggagcgggg cttcgagagc gaggaccctg tgcaaagaga tgtcgtaccc agctaacttg
     1561 ccggcggccg ggtccggccg ctatcaccct ttccaacgcg gccggggtta gacttgagga
     1621 cagaaaacag gagggtaagt tcacggtgac aagacggtcg aggagccact ctctggcggt
     1681 gctcgtcctc gccgttgccg tagccgttac gctggtcctc ctggtcggat gcagtgctgg
     1741 aaccgacgag tccttcgagg gagccgcctc tggcgaaacc acaggcccga tagggatagt
     1801 gggcgggaag atcacggtcg cctccaacat cgcctatccg cctttcgaat cctcccccaa
     1861 aggcagaccg cgaggattcg atattgacct catgaacgag atcgggaaga gggttggctt
     1921 cgaggtcgaa tacgagaacg tccgcttcga cactatcctg cggggtctgg acaccgacct
     1981 cttcgacgct gctatctcgg cgatgaccat taccgaagat cgcgagcgac agatagactt
     2041 ctccgatgct tacttcaacg ccgaccaggc gctcctggta gcgagcgatt cggaagtcca
     2101 ctctatcgat gagctcgccg aggagcccgt cggcgtccag gccggctcga cgggacagat
     2161 caaggccgaa gagctcctcg acggcgagca gataggggag atcatgcctt acagaaccat
     2221 aggtgatgcc ttcgcagcgc tcgaggatgg gaaggtcgag ggggttatct acgacctctc
     2281 tgccgcgcac aggaagatcg tagagagcga cagggagatc aggttcgtcg aacccatctc
     2341 gacgggcgag cagtacggga ttgcgttccc caaagacagt ccgctagtcg accccgtcaa
     2401 ccgggctctg gccgagatca aggacgacgg cacctatgag aagctctaca agaagtggat
     2461 cgggatgcct cccgaggaga taccgtagag agcttgtcag cttgtaagct ttcccggttc
     2521 tggccccgct cggcagcgca cgagaagtgc agtctgccgc ggtcgggttt atgctcatgg
     2581 ctacgtgatg ggatcactgg ctgaaatgct gagaagccgc cgaccgaagg gaggcggcgg
     2641 gctgacaagc ggaggcgtca gccagagcgt gctgaaagcc tgcgaagcag gcgtgctgaa
     2701 atgacctcca tcctcaggtc catactctcg atctccgcgg cgacgatcct ctccagggcg
     2761 acggggtacg cgaggtggat ggcccaggca gcggccctcg gcgccgggta cgtggccgac
     2821 gcttacacgg cctctatcat tttgccgagt ctgatctacg agctcttcat gggtggaata
     2881 ctctattcga tcttcatccc cgtcctcgta gaccgcatta cgaaggtggg agaggaggac
     2941 gccaggcggc tgaccaacgc ccttttcacg ctggtcttgc cgctaatggt gatactttcg
     3001 gtcttgggta tcgtgttcgc caggccgctg ctgctactcg tcaccgactg gcaatcgggg
     3061 ctctccccca ccgacgccga acggacgttg gatctcgcct tgctgttctt ccggatcttc
     3121 atcctgcaga tgttcttcta cggcataagc accatcggca cgggcgtctt gcaggcccac
     3181 aggcacttct ttttgcccac cttcgccccc gtgctgaaca acctgatggt cgtagcatcc
     3241 ttcctcgcct actccctgct cgtaggctac gaccagatcc tagccttcta cgtgctcgcg
     3301 ataggggttg cggcgggggt cgcggtcatg gccctggcgc tggtgccgac catgtggagg
     3361 ctcggataca ggccgcgccc acagtttggc caccccgcgc tgctccccac ggccaggctc
     3421 gccggaccga tggtcattct ggtcgccgcc tccgtgggct tccagctctt cggtgcttac
     3481 ctggccacgg ggttcgaggc actctcggag ctaaactacg ccttcaccat cttctcgctc
     3541 ccctacggcg tctttgtggt cgccatcgcg accgcgatca tgcccgacct ttccgagaag
     3601 cactccctta gggacgccga cggttaccgc gagacgttct ccttcgggct taggacgatg
     3661 atcttcgtcg tcataccatc ggcggtggga atgatctcgc tctccaggcc catcgtcggg
     3721 ttgctctacg agcgtggaag cttcgatgcc gaggataccg agaccgtctc caggttgctc
     3781 gtggtcttcg ctgcgggatt gctcggctac tcggtgtact tcttcctcgt gcgggcattc
     3841 tactcgcggc agaacacgaa gaccccggcc gcgttgaacg tcgccatctt cttgctctac
     3901 gcggtcctcg cctacggcct ctcgcgcgtc cttggcgtga caggcgtggt gctcgctctt
     3961 tccatagcct acgccgtgct cgccatcctc ggcctcgcgg cgacgcgccg ggagataggg
     4021 ctgatagaag gccggcgcct ccttcgctcc ctcctcaaaa tcctggcggc cggcgcactt
     4081 atgtacgtcg ttgccaggac gggaacctta ctcctcgggg ccggctcgga cctgacagag
     4141 cgcctcttca tcctcgttac aattggcggc gcctcgctgg ccgtctacat cggggtagcg
     4201 ttccttctga ggacggagga gctagcgtcc gctttcaccc tacttcggcg tcgttccgcc
     4261 gggaagagtg cgggttagga accttcctgg gacttccaaa cggcactcga aagattcttg
     4321 atgaggtcct gcccctggta cacgtcggcc gtgccgtgct tgctgaggat cgctatgacg
     4381 tagcggcggt cgtcgttctc gacgatcccc acctcgtcgt agaccctgta gagccagcc
//
LOCUS       DATMJA010000409         4000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_350945, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000409 DATMJA010000000
VERSION     DATMJA010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350945"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2615)
                     /locus_tag="VNT12_09730"
     CDS             complement(<1..2615)
                     /locus_tag="VNT12_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HWI46438.1"
                     /translation="MNTRERHRLDRHKVRVEELKLWRNAKESRIEEWRFAADDGDAKE
                     IGLGDFWPEEALPASFSAEARIPEDWAGSPVELELWLGGEGFVSLSTGFSGGLNPFHR
                     SFPVTQKAAGGETVGIEAEVVPRGMFGSNVAEPQLRRSSLVVPETGVRALERDLGLIV
                     EVCEQLGDHEVVTHLLDVVDAAFTNLFSLWPSASEVALTRYLEAYAESTDGGPWSLPP
                     APRDVEPLSEETRSAVREARAMVASGLKELKAEYPPVGRLALTGHAHLDLAWLWPVSE
                     TMRKGRRTFASVLSLMDRYDDFVFNQSSAQLYKWIETGSPEIFESVRRRVEEGRWEPV
                     GGSWVEPDCQIPSGESMVRQMLYGQRYFQEKFGHRSRVAWLPDAFGFSPALPQLLRGA
                     GMEGFFTYKLNWSETNEFPYDLYEWEGIDGSTVIAHDFENPGQDYNGNITPRDIYGTW
                     RNFEGKRHHPESLFSFGWGDGGGGPSEQMLENYARMKSFPAMPRLRMAHVDEFFDSLP
                     REGLPRWTGELYLELHRGTLTTQARVKKLNREAEHRLLEAEALSTLAALHGGDYQREG
                     LETAWKTLLLNQFHDILPGTSIGEVYEETHEELADVVRTARKLRDRALSPPPDAEPAL
                     LVANVSLNPRPLSVLLEPESRPLGAARVAGADGRPLPAQETESGGLLVHAPDLSVPGL
                     GWLSLTAQHESALARRQTRDVSAEAAGGRAVLDNGLLRVEIGEDGTLHGVYDREAGRE
                     VLDGRANQLWAYTDKPRNWEAWDVDEGYEQEGEEISGVESVEVVEYGPLRASVRVVRR
                     WRGSTIAQTYRLLSGSRRVDIESYVDWHERRVLLRTLFPLAVRSHETTCETMFGAVRR
                     PTHHNTPWDATRFEVS"
     gene            2790..>4000
                     /locus_tag="VNT12_09735"
     CDS             2790..>4000
                     /locus_tag="VNT12_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI46439.1"
                     /translation="MSEKVYRSELTPVSFLRRSAYMFPEKPAVVYGERRYSYSELEER
                     VDRLSSRLRDGGLRKGDRVAFLCPNTPPMLEAHFAVPAAGLVLVAINTRLGKDEVSYI
                     VEHSGAKMVFVDAELEELLADVDGVETVRIDDTGEQGDPYEDYLAEGSPEPAPDVLED
                     EEETISINYTSGTTGRPKGVMYTHRGAYLSALGNAIEIGMGYETKYLWTLPMFHCNGW
                     TFPWAVTAVSGTHVCLRKVEPPRIWELFEDEGITHYCAAPTVQIGIVNEEGAHRLETS
                     VTAAIAGAPPSPTLLGGLIELNIRPMHIYGLTETYGPMTTSGVHPEWEELDMEERARL
                     MARQGQGYTTADLVRVVDENMNDVERDGETMGEIVMHGNMVAKGYFENEEATQEAFEG
                     GWYHSGDVAVW"
BASE COUNT          702 a         1313 c         1279 g          706 t
ORIGIN      
        1 gagacttcga aacgggtggc gtcccagggt gtattgtgat gcgtcggccg ccgcacggcc
       61 ccgaacatcg tctcgcaggt ggtctcgtgg gagcggacgg cgagcgggaa gagcgttctg
      121 aggaggaccc tcctctcgtg ccagtcgacg taggactcga tgtccacccg tcgcgagccg
      181 gagaggagcc tataggtctg cgcgatggtc gagcctcgcc atctgcggac cacgcggacg
      241 gaggcgcgga gcggcccgta ctccacgacc tctacgcttt cgacgccgga gatctcctcc
      301 ccctcctgct cgtacccctc gtccacatcc cacgcctccc agttgcgggg cttgtccgtg
      361 taggcccaga gctggttggc gcgtccgtcc aggacctcac ggccggcctc cctgtcgtag
      421 acgccgtgga gcgtgccgtc ctcccctatc tccacacgca agagaccgtt gtccagaaca
      481 gcgcgtccac ccgccgcctc agcgcttaca tcccgggtct ggcgtcgggc cagcgcagac
      541 tcatgctggg ccgttagcga aagccagccg agacccggca cgctgagatc tggggcgtgt
      601 acgagcagtc ctccgctctc tgtctcctgg gcaggcagcg gtctgccatc cgcgccggcg
      661 acgcgcgcgg cgcctaaggg cctactctcg ggctcgagca ggacggagag aggacgcggg
      721 ttcagtgaga cgttggcgac gagcagcgcc ggctctgcat ccggcggggg actaagcgct
      781 ctgtcgcgca gctttcgggc cgtcctgacg acgtccgcga gctcctcatg ggtctcttca
      841 tagacctccc ctatcgaggt ccctggcagt atgtcgtgga actgattgag caggagtgtc
      901 ttccaggcgg tttcaagccc ttcgcgctgg taatcgccgc cgtggagcgc ggccagcgtg
      961 gagagggctt ccgcctcgag caggcggtgc tcggcctcgc ggtttagctt cttgaccctg
     1021 gcctgggtag tcagagtacc cctgtgcaac tcgaggtaga gttcgccggt ccaccttggg
     1081 agtccttctc tggggagact atcgaagaac tcgtcgacgt gggccatgcg cagacgcggc
     1141 atcgcgggaa aactcttcat gcgggcgtag ttttcgagca tctgctcgct cgggccgccg
     1201 ccgccgtcgc cccacccgaa ggagaagagg ctctcggggt ggtggcgctt gccctcgaag
     1261 ttgcgccagg tgccgtagat atcgcggggt gtaatattgc cattgtagtc ctggccgggg
     1321 ttctcgaagt cgtgggcaat taccgtgcta ccgtctatgc cctcccactc gtagaggtcg
     1381 taaggaaact cgttggtctc gctccagttg agcttgtagg tgaagaagcc ctccatcccc
     1441 gcgccccgca gaagttgcgg cagcgccgga gagaatccga aggcgtcagg gagccaggcc
     1501 acccgcgagc gatggccgaa cttctcctgg aagtacctct gtccgtagag catctggcgc
     1561 accatagact cgcctgatgg gatctggcag tcaggctcca cccacgagcc gcctacaggc
     1621 tcccaacgcc cctcctcgac ccgcctcctt acgctctcga agatctctgg cgacccggtc
     1681 tctatccact tgtagagctg ggccgaagac tggttgaaga cgaaatcgtc gtagcggtcc
     1741 atgagcgaga gtacgctcgc gaaggtcctt cgtccctttc gcatggtctc cgagactggc
     1801 cagagccagg cgaggtcgag gtgcgcatgg cccgtcaggg cgaggcgccc gaccggcgga
     1861 tactcggctt tcaactcctt caggccggag gcgaccatcg ctcgcgcctc ccgcacggca
     1921 ctccgcgtct cttcggagag gggctccacg tctctaggcg cgggtggcag actccacgga
     1981 ccgccgtcgg tcgactcggc gtaggcctcc aggtagcggg taagcgcgac ctcgctcgcc
     2041 gagggccata aggagaacaa gtttgtgaac gcggcgtcga cgacatcgag gaggtgagtg
     2101 acgacttcgt gatcccctag ctgctcgcag acctcgacta tgagcccgag gtcacgctcc
     2161 agggcgcgaa ctcccgtctc cgggaccacg agcgaggacc gccttagctg tggttcggcg
     2221 acgttcgagc cgaacatccc ccttggcacg acttcggcct cgatcccgac cgtctccccg
     2281 cccgcggcct tctgcgtcac ggggaagctg cggtggaaag ggttgaggcc accgctgaac
     2341 cccgtcgaga gactaacgaa accctcgcca ccgagccaga gttccagctc gacgggagag
     2401 ccggcccagt cttcgggtat cctcgcctca gccgagaagc tcgccggcag cgcctcctcg
     2461 ggccagaagt cacccagccc gatctccttc gcgtccccgt catcggccgc gaagcgccac
     2521 tcctcgatgc gactctcttt cgcgttgcgc cacagcttca gctcctcgac gcggaccttg
     2581 tgacgatcca gacggtgtct ctcgcgggtg ttcatttatc tccctctact cggacgcggc
     2641 agcagacaca gatattagcc gctttgccct acgcgcaccg gcctcgagcc gtgggacatg
     2701 cattgggcca ccttgctatc gagtccgacg tggcaaggaa ggagccacga cctatatact
     2761 ctgtagggca gatacgaaag gagaattctg tgagcgagaa ggtctaccgc agcgaactta
     2821 caccggtcag cttcctcagg cgcagcgcct acatgtttcc cgagaagccc gccgtcgtct
     2881 acggcgaacg gcgttactcc tacagcgagc tcgaggagcg ggtcgaccgt ctgtcctcgc
     2941 ggctacggga cggcggtctg cggaagggcg accgcgtcgc cttcctctgc cccaacacgc
     3001 cgcccatgct ggaggcccac ttcgccgtac ccgccgccgg gctcgtgctc gtcgccatca
     3061 acacccgtct cggcaaggac gaggtctcct acatcgtcga gcactcgggc gcgaagatgg
     3121 tcttcgtgga cgccgaactg gaagagctgc tcgccgacgt cgacggcgtc gagaccgtgc
     3181 gcatcgacga caccggggag cagggggacc cgtacgagga ctacctggcc gaaggctcgc
     3241 ctgaacccgc gcccgacgtg ctagaagatg aagaagagac catctctatc aactacacct
     3301 cggggacgac gggccgtccg aagggcgtga tgtacacgca ccggggggcg tatttgagcg
     3361 ctctcgggaa cgcgatcgag atcgggatgg gctacgagac caagtacctc tggaccctcc
     3421 ctatgttcca ctgcaatggg tggacgtttc cgtgggccgt gaccgcagtc tcgggaacgc
     3481 acgtgtgcct caggaaggtc gagccgccgc gcatctggga gctcttcgag gacgagggca
     3541 tcacgcacta ctgcgcggcc ccgaccgtcc agatcggcat agtcaatgag gaaggggctc
     3601 accggctgga gacgtctgta actgccgcca tcgcgggcgc tccgccttcc ccaacgttgc
     3661 tcggcggcct catcgaactg aacatccgtc cgatgcacat ctacggcctc accgagacct
     3721 acggcccgat gaccacgagc ggggtccatc ccgagtggga agagcttgat atggaagagc
     3781 gggcgcgcct catggcccgt cagggtcagg gctacactac cgccgacctc gtgcgcgtgg
     3841 tagacgagaa tatgaacgac gtggagcgcg acggggagac gatgggtgag atcgtgatgc
     3901 acggcaacat ggtcgccaag ggttacttcg agaacgagga ggcgacgcag gaggcgttcg
     3961 agggtggctg gtaccactca ggtgacgtgg cagtgtggca
//
LOCUS       DATMJA010000410         8793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_113683, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000410 DATMJA010000000
VERSION     DATMJA010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113683"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VNT12_09740"
     CDS             <1..1074
                     /locus_tag="VNT12_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWI46440.1"
                     /translation="GIAPPSARPGVSHTSVREPLGVVGLITPWNFPLAIPTWKMAPAL
                     ICGNTIVMKPAGNASLNAAALVRILAEAGLPDGVLNMVTGPGGAVGDALVTDPRVKGI
                     SFTGSTSVGLGIQGKAVGKKVQLEMGGKNPYIVMEDADLSDAAAKISFGAFGYAGEKC
                     TASSRAIVVQEVYDEFIEELKSATEAMKVGDPLEEDTDIGPVVNKSQYDSILDALETA
                     KNDGRVVLDGGATGSEDDGYYIAPAIVADVDNRSETAQEEIFGPVLAVIRASDFDEAV
                     DLANDTRYGLTAGIATKSLRYAHEFMQRSETGLVNVNLPTAGLEFQVPFGGNKESGVG
                     GREQGPAALDFYSSWKTVSMMPL"
     gene            1086..1538
                     /locus_tag="VNT12_09745"
     CDS             1086..1538
                     /locus_tag="VNT12_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI46441.1"
                     /translation="MRARGFTHVSISATDLEESVRFYTDFFGMEEVPSPDFSGPVRWL
                     RVGDLQLHLFLDKDPAPQRHHFALDVDDFEAAHAKAEELGVRDGGRYSSVRELPDGAV
                     QMYLRDPAGNLVEVNWPDVTTLDREVIPEIQKIGGDKDAVLYLEPPGR"
     gene            1576..2061
                     /locus_tag="VNT12_09750"
     CDS             1576..2061
                     /locus_tag="VNT12_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HWI46442.1"
                     /translation="MTQERTEQVISDLGLPDQAIQSDELPWVPQGERVWFKPLRFDLA
                     TGRWINVLKVEGSGKVNRHRHTGGQVMGFCIKGSWHYLEREWVARPGTLVYEPPGDIH
                     TLVVDEGVEEMQTLFILEGTVQYIDDNDDLIYQDDVFSKLERYLKFCDEQDIEPQDLR
                     Y"
     gene            2061..2831
                     /gene="kduD"
                     /locus_tag="VNT12_09755"
     CDS             2061..2831
                     /gene="kduD"
                     /locus_tag="VNT12_09755"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HWI46443.1"
                     /translation="MGILDDFSIDGKVALVTGAGRGLGQGIALGLAEAGADVAALDII
                     SVDETGEMVHNEGRDFHAMNVDLLETDAKLAADVVEECVSSLGCLDILVNNAGIIRRA
                     PALEFGEEDWEAVLKINLSAAFYLSQAAARHFDEEGRGGKIINTASVLSYEGGVIVPS
                     YSASKAGIANLTRALANEWAPLGINVNAIAPSYFTTELTSALREDAERSEALLARLPA
                     GRFGEPEDLKGAAVFLASVAADYVHGTVLPVDGGWLAR"
     gene            complement(2835..5636)
                     /locus_tag="VNT12_09760"
     CDS             complement(2835..5636)
                     /locus_tag="VNT12_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HWI46444.1"
                     /translation="MKQRVWDFQEVGRLPTSGDNVAMATRRLEAGTCVSYEDSEFTVG
                     HTVLEGHRFAVEPVAEGEDLLSWGLRFGCATKDISPGDYACNEKILRVLRERFRVSPR
                     RGEDEDPEGTLDQGGGRVPGGYRDAGLNLPEEPNFRDAELEPYVLDEEHFRPGEQVPL
                     HDEPRTFMGYRRGPGRGVGTRNYIVVMGVTSRLTGFVRALELEMNGVADAYENVDGIV
                     CVAHTEGGEERTPNNLDLLLRTLSGFMVNPNVGAVLVLDHGGEEAVTNRMLGAYLEEN
                     GYPIDDVPHEFMSLEGSFRRDLERAKSVVHGWLEEVDEARRTEEPASELKISLQCGGS
                     DAFSGISANPLVAWVSREVVRNGGIANLAETDELIGAEHFVLKNVKDLETARRFLTTV
                     ERFKERVSWHGQTAEDNPSGGNNYRGLYNISIKSLGAAMKKHPDVRIDHIIEYAERMD
                     EGGFYFMDSPGNDLESVAGQVASGANMIFFTTGNGSITNFPFVPTIKFVTTTGRYELL
                     SKDMDVNAGAYLGGTPMDELGRETFERTLRAASGERTVGERAGHSQVSIWRDWKQTND
                     ENLDRLREAQEPDGEPLPVKESAADVEFSFEAIKSGRGPVLDQVALVMPTSLCSGQIS
                     RRIANGLNERDATQGKVTRFVALPHTEGCGVSAGSAETIYSRTVLGHLASPTVKFGLL
                     LEHGCEKTHNDYFRNRLEEAGLDPDRFGWASVQLDGGIDSVVGKVEDWFEETLDGAED
                     LEYEGAGSEALRLGLYAAGHISDEAAMSLAEITLAVVNSGGTVVVPERAAVLSSPVYR
                     KAVLGDRSVENTLAYGQAVPVGKQGLHVMEAPTDHWVETATGLGATGIEVMLAHLTGH
                     PLQAHRMIPLVQASSDPETLERYAEDLDVHLEGDPSGWTERMLETVAAVASREYVPKL
                     FEAGNTDFQFTRGLLGVSM"
     gene            complement(5814..6023)
                     /locus_tag="VNT12_09765"
     CDS             complement(5814..6023)
                     /locus_tag="VNT12_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46445.1"
                     /translation="MNDAMQQRLVTILAAAIAYLITQYVVERLIDIPDERGIKDDAIE
                     ALLKGATTATSTILASVLVRRLLRS"
     gene            complement(6123..7073)
                     /locus_tag="VNT12_09770"
     CDS             complement(6123..7073)
                     /locus_tag="VNT12_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWI46446.1"
                     /translation="MQGSSLDLPTGYYLERDPDILTLRRLDGSIVSAFSARGAAPEAV
                     RRMVEETVQGEHSAAVQPSLQVRFFGHFEMLCDGEAMPLGRSGKALTILKYLLANHTR
                     PVSQDHLMGWLWPESNLKKARWSLNSAIHGLRKLLSGCSSSLSMNYVFLEDGYYRLSP
                     DVRVTTDVDEFDKHHERGRKLEKDLRMREAAIEYEKAIELYRDDYLIEDLYEDWTMVE
                     RERLANAYMDLLGRLAVHYMEVGQYQESIRACYRVLEKDRCHEDSYRLLMQCYAHLGL
                     RARALHQYRMCEQILGQEYGTSPSPETRALYLKLLRDESA"
     gene            complement(7357..>8793)
                     /locus_tag="VNT12_09775"
     CDS             complement(7357..>8793)
                     /locus_tag="VNT12_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWI46447.1"
                     /translation="LEGILGWTVEAAHTVGGGPYAHVAALKGNYHRTILEGDFDVCPS
                     WWHPSIQRLVLQSCREGDTVRSEEEIHGIEGFMAIPLGTGEDEKWGAVIVGGGEYGAE
                     EERVLKLLAEGIGPALEDAGDAPGGLDQLTGLPNRASLHRVLRRELSYGRPLTVLAVQ
                     PTIVRDHGLATEDVLFQRIGRRLADGRQRAFRYGDEVFVVLSGAGESRARQAALALRQ
                     HVSEEAGGPDNLPLTASVGWVFAEEGDERPVLDAALRALERAKGLPEGIFGLPAGAQN
                     PGPFVEGENQVLGIAWTLVEALEAKDPYIKEHLRAVSGLALRIGSEISLPGEQMEALT
                     SGALLHDVGKIGIPDRILQKSGRLTEDEYAEIKRHPLLGVSILTPATELASALPVVKH
                     HHERFDGKGYPDGLRGEDIPLIARIVSVADAFDSMIRARPYGYGISRKAALREIEENS
                     GTQFDPRIVRALLEVVYAPGDRQADSAG"
BASE COUNT         1595 a         2850 c         2681 g         1667 t
ORIGIN      
        1 ggtatagccc cgccctccgc gcggcccggc gtgagccaca cctcagtcag ggagccccta
       61 ggggtggtgg gcctcataac cccgtggaac ttcccgctgg cgataccgac gtggaagatg
      121 gcgccggccc tgatctgcgg caacaccatc gtgatgaagc cggcgggcaa cgcctcccta
      181 aacgcagcgg cgctcgtccg gatactcgcg gaggcgggcc tgccagacgg agtcctgaac
      241 atggtgaccg gacccggcgg agcagtaggg gacgcgctcg ttaccgatcc gcgcgtcaaa
      301 ggcatctcgt tcacgggctc gacgagcgtg ggcctcggca tccagggcaa ggccgtgggc
      361 aagaaggtcc agctcgagat gggcggcaag aacccctaca tcgtgatgga ggacgccgac
      421 ctctccgatg cggcggccaa gatctccttc ggcgccttcg ggtacgccgg cgagaagtgc
      481 acggcctcga gccgggccat cgtcgtccaa gaggtctacg acgagttcat cgaagagctc
      541 aagagcgcga ccgaagccat gaaggtcggc gacccgctcg aagaggacac ggatatagga
      601 ccggtcgtca acaagagcca gtacgacagt attctcgatg ccctggagac ggccaagaac
      661 gacgggcgcg tggtactcga tggcggggca accggctctg aggacgatgg ttactacatc
      721 gccccggcca tcgtggccga cgtggacaac cgttcggaga ccgcgcagga ggagatcttc
      781 ggccccgtcc tcgccgtaat aagggctagc gacttcgacg aagccgtcga cctcgccaac
      841 gacacccgct acggcctgac cgcggggata gcgacgaagt ccctgcgcta cgcccacgag
      901 ttcatgcaac gctcggagac gggcctcgtg aacgtgaacc tgccgaccgc gggcctggag
      961 ttccaggtgc ctttcggtgg caacaaggag tcgggcgtcg gtggtcggga acagggtccg
     1021 gcggcgctgg acttctactc gtcgtggaag accgtctcca tgatgccgct gtagggagcg
     1081 tcggcgtgcg ggcgagaggg ttcacccacg tcagcatcag cgccaccgac ctggaggagt
     1141 cggtccgctt ctacacggac ttcttcggga tggaggaggt cccgtcgccc gacttctccg
     1201 ggccggtgcg ctggctgcgg gtgggtgatc tgcaactcca cctcttcctc gacaaagacc
     1261 ccgcgccaca gcgacatcac ttcgcgctcg acgtagacga cttcgaggcc gcgcacgcga
     1321 aggccgaaga gctgggcgtg agggacggcg gacgttactc gagtgtccgt gagttgccag
     1381 acggtgccgt ccagatgtat ctgcgcgatc cggcgggcaa cctcgtcgag gtcaactggc
     1441 ccgatgtgac gacgctcgac cgcgaggtca taccggagat ccagaagatc ggcggcgata
     1501 aagatgcggt gctctacctc gagcctcccg ggcgctgaat agtaggacat caggccacga
     1561 agtaaaggag aatttatgac gcaggaacgc acggagcagg tcatctccga tctcgggctg
     1621 ccggatcagg ccatacaaag cgacgagctg ccgtgggtgc cgcaaggcga gcgcgtctgg
     1681 ttcaagccct tgaggttcga ccttgcgacg gggcgttgga tcaacgtcct caaggtcgag
     1741 ggctcgggca aggtcaaccg ccaccgccac acaggcgggc aggtcatggg cttctgcata
     1801 aagggctcct ggcactacct ggagcgcgag tgggtcgcgc ggcctggcac gctcgtctac
     1861 gagccgcccg gcgacatcca cacgcttgtt gtcgacgaag gcgtcgagga gatgcagacc
     1921 ctcttcatac tcgaaggtac ggtccagtac atagacgaca acgacgacct gatctaccag
     1981 gacgacgtct tctcgaagct ggagcgctac ctgaagttct gcgacgagca agacatcgaa
     2041 ccccaggacc tccgctacta gtgggcatcc tcgacgactt cagcatcgac ggcaaagtcg
     2101 cgctggtaac aggtgcaggg cgcggcctcg gccagggcat cgcactgggc ctcgccgagg
     2161 ccggcgcaga cgttgcggcc ttggacatca tttccgtgga tgagaccgga gaaatggtcc
     2221 ataacgaggg ccgcgacttc catgccatga acgtcgatct attggagact gacgcgaagc
     2281 tcgccgccga tgtcgtcgag gaatgcgtaa gctcgctcgg atgccttgac atcctggtca
     2341 acaacgctgg catcatccgc cgtgctccgg cgctcgagtt cggcgaggag gattgggaag
     2401 ccgtcctaaa gatcaacctg agcgccgcct tctacctctc ccaggccgcc gccaggcact
     2461 tcgacgagga gggcagggga ggtaagatca tcaacaccgc ctcggtcctc tcctacgagg
     2521 gcggcgtgat cgtaccctcc tactctgcgt ccaaggcggg catcgccaac cttacccgcg
     2581 cgctcgccaa tgaatgggcg ccactgggga tcaacgtcaa cgccatcgcc ccgagctact
     2641 tcaccacaga gctgacgagc gcactgcgcg aggacgccga gaggtccgag gccctgctcg
     2701 cccgcctccc cgccgggcgc ttcggcgagc ccgaggacct caagggcgct gcggtcttcc
     2761 ttgcctccgt tgccgcggat tacgtccacg gaacggtcct acccgtagac ggcggctggc
     2821 tggcacgcta gcgactacat cgagacgccg agcagaccgc gggtgaactg gaagtccgtg
     2881 ttgcccgctt cgaacagttt gggaacgtac tctcgcgagg ctacggccgc gaccgtctcc
     2941 agcatccgtt cggtccagcc ggacgggtct ccctcgagat gcacgtcgag gtcctcggca
     3001 tatctctcta gcgtctcggg gtcggatgag gcctgtacta gcgggatcat acggtgcgcc
     3061 tgcaaaggat gccccgtgag gtgcgccagc attacttcga tcccggttgc cccgagcccg
     3121 gtcgcggtct ccacccagtg gtccgtcggc gcctccatga cgtgcagccc ctgcttgccc
     3181 accgggaccg cctgcccgta agcgagggtg ttctccacag atcgatcgcc gagcacggcc
     3241 ttgcggtaga ccggcgaaga gagaaccgca gccctctccg gtacgacgac cgtaccgcct
     3301 gagttgacaa cagcaagcgt tatctccgcc aggctcatgg cggcctcgtc cgaaatgtgc
     3361 ccggcggcgt atagaccgag ccgcaaggct tccgagcctg cgccctcgta ctccagatct
     3421 tcggcaccgt ccagggtctc ctcaaaccag tcttcgacct tcccgacgac gctgtctatg
     3481 ccgccgtcga gctgcacact cgcccagccg aagcgatccg gatccaggcc tgcctcttcg
     3541 agacggttcc ggaagtagtc gttgtgggtc ttctcgcagc cgtgttcgag gagcagtccg
     3601 aacttcacgg tggggctggc gagatgtcct agcacggtgc gggagtagat ggtctcggcc
     3661 gagccggcgg agacgccgca gccctctgtg tggggcaggg cgacgaagcg ggttactttg
     3721 ccctgggtgg catcacgttc gttcagcccg ttggctatgc ggcgtgagat ctggcccgag
     3781 caaaggctcg tcggcataac gagcgcgacc tgatccaaga ccggcccacg cccgctcttt
     3841 atggcctcga aggagaactc gacgtcagcg gcactctcct tcacgggcaa cggctcgccg
     3901 tccggttcct gcgcctccct gagccggtcc agattctcgt cgttcgtctg cttccagtcg
     3961 cgccagatgg agacctggga gtggccggca cgctctccga cggtcctctc gcccgaggcg
     4021 gccctgagag tccgttcgaa tgtctcacgg cccagctcat ccatcggcgt accgcctagg
     4081 taggcgcccg cgttcacgtc catgtcttta gacaagagtt cgtagcggcc tgtggtggtg
     4141 acgaacttta tggtcgggac gaaggggaag ttggtaatgg agccgttgcc cgtggtgaag
     4201 aagatcatgt tagcccccga ggcgacctgc ccggcgacgc tctccagatc gttgccgggg
     4261 ctgtccataa agtagaagcc gccctcgtcc atccgttcag cgtactcgat aatatggtcg
     4321 atccgcacgt caggatgctt cttcatcgcc gccccaagag acttgatgga gatgttgtag
     4381 agcccccggt agttgttgcc tccagatggg ttgtcttcgg ccgtctgccc gtgccaggag
     4441 acgcgctcct tgaaccgctc gaccgtggtg aggaaacgcc gcgcggtctc gaggtccttg
     4501 acgttcttta gaacgaagtg ctcggccccg atcaactcgt ccgtctcggc cagattggcg
     4561 atgccgccgt tcctgacgac ctccctgctc acccacgcca ccagcgggtt tgcagagatc
     4621 ccggagaagg cgtcagaccc gccgcactgc aaggagatct tcaactcgga agctggctcc
     4681 tcggtgcgcc tcgcctcgtc cacctcttcg agccatccgt gcacgacgga tttggccctt
     4741 tcgagatccc gcctgaagct cccttccagg ctcatgaact cgtgcggcac atcgtctatc
     4801 gggtagccgt tctcctccag gtacgccccg agcatcctgt tcgtcacggc ctcctcaccg
     4861 ccatggtcga gcacgagcac ggcccccacg ttcgggttga ccatgaagcc gctcagggtc
     4921 cttaagagaa ggtccaggtt gttcggcgtg cgctcctccc cgccctccgt gtgggcgacg
     4981 cagacgatgc cgtctacgtt ctcgtatgcg tcggcgacgc cgttcatctc gagctccagc
     5041 gccctgacga acccggtgag cctcgaggtg acgcccatca cgactatgta gtttcgcgtc
     5101 ccgacgcccc tgccaggacc acgcctgtag cccatgaagg tgcgcggctc gtcgtggagg
     5161 ggaacctgct cgccgggacg gaaatgctcc tcgtcgagga catagggctc cagctccgca
     5221 tcgcggaagt taggctcttc cggcaggttt aggccagcat cgcgataccc tcccggaaca
     5281 cgtcctccgc cttgatccaa ggtaccttca ggatcctcgt cttcgccgcg tcgaggcgag
     5341 acccggaaac gctcgcgtag gacacgcagg atcttctcgt tgcacgcgta atcgccgggg
     5401 gagatgtcct tggtggcgca tccgaagcgg aggccccagg agagtaggtc ctctccttct
     5461 gctacaggct cgacggcgaa gcggtgccct tcgagtaccg tgtgtccgac ggtaaactcc
     5521 gagtcttcgt atgacacgca cgtccccgcc tcgagcctgc gggtcgccat tgccacgttg
     5581 tcgcccgatg tggggaggcg tccgacttcc tggaaatccc atactcgttg tttcacagcg
     5641 atgcctccct tatgctttca gagatacgtt ctcgcgacga gtgtacctta gcgttcccta
     5701 ctgctctcac gccccgatct tccggccgac gatgacatac acctcgcggg acaccaacaa
     5761 gaaagccgga accccaccgc gggctccggc gattctagag cttggcgcgg aattcaggac
     5821 ctgagcagcc gtcgcacgag gacggaggcc aggatggtcg aggtcgcggt cgtcgctccc
     5881 ttgagaagcg cctctatggc gtcgtctttg atcccgcgct catcgggtat gtctatgagc
     5941 ctctcgacaa cgtactgggt aatgaggtag gctatcgcgg ctgccagtat ggtcacgagc
     6001 ctctgctgca tcgcgtcgtt catgtgctcc cttccgcctt ttagggtctg ctctggtcgg
     6061 tacccacgat aggctctgcg aaaacccccg ggttctggtc ttcgagtccg gtccggcagc
     6121 tctcacgcgc tctcgtctct caggagtttc aggtagaggg ctctagtctc cggcgagggt
     6181 gaagtaccgt attcttgtcc gagtatctgc tcgcacatcc tgtactggtg cagcgccctg
     6241 gcccgcaggc cgaggtgagc gtagcactgc atcaggagac ggtaactgtc ttcgtggcag
     6301 cgatccttct ccagtactct gtaacaggcg cgaatgctct cctggtattg cccgacttcc
     6361 atgtagtgga cggccagccg gcccagaaga tccatgtaag cgttggccaa ccgctctcgc
     6421 tcgaccatgg tccaatcctc gtacaggtcc tctatcaggt agtcgtcccg atacagctcg
     6481 atggccttct cgtactcgat ggccgcctcc cgcatcctga ggtccttctc cagtttacgc
     6541 cctcgctcgt ggtgtttatc gaactcgtcc acgtccgttg tcaccctgac gtccggagac
     6601 agacgatagt atccgtcctc gagaaagacg tagttcatgg agagagaaga cgaacacccg
     6661 ctcaatagct tgcggaggcc gtggatagcg gagttaaggg accaccgggc tttcttgagg
     6721 ttggactcag gccacagcca gcccataagg tgatcctgcg agacgggacg ggtatggttg
     6781 gccagcaggt atttgagtat ggtcagcgct ttgccgctac ggccgagggg catggcctca
     6841 ccatcgcaaa gcatctcgaa gtgtccaaag aaacgcacct gcaaagaagg ctgaacagcg
     6901 gcggaatgtt ctccttggac ggtctcctcg accatccgcc gcacggcttc gggggcggcg
     6961 ccacgagccg aaaacgcgct gactatggat ccgtcgagcc ggcgtagggt caatatgtcc
     7021 ggatctcttt ccagatagta accggtcggc agatctaacg atgatccctg catgtctatc
     7081 tccagatcgc tgtcctcggc ccaaaggccg cggttcgcct ctccctaacc ctctttgcag
     7141 tcgttacgcg tagtttttct gtggaccaac cgctgcggct cctttcccac gggacgaaac
     7201 atataaggct ttggctcgcc cgaaatcaca tagcgggttc agcatataac ggatatgggg
     7261 gtgcccgtat ccctcaaatg agtgattctt catccgtttg gaaaattata tggtagaggc
     7321 accggtcggg gcaaatcgcg cttccggtgc gcgtcgctaa ccggcggaat cggcctgccg
     7381 gtcgccggga gcgtatacga cctcaagaag cgcccttacg atacggggat cgaactgggt
     7441 tcccgagttc tcctcgatct cccttagagc ggctttgcgg gagatgccgt aaccgtacgg
     7501 cctggcacgg atcatggaat cgaaagcgtc ggccaccgag actatccttg ctatcaaggg
     7561 aatatcctcg ccgcgcagcc cgtccgggta tcctttgccg tcgaagcgct cgtgatggtg
     7621 tttgacgacc ggcaacgcgg atgcgagctc tgttgcaggg gtgaggatcg agacaccgag
     7681 taacggatgc cgctttatct ccgcgtactc atcctcggtc aggcgacctg acttttggag
     7741 gatgcgatcc ggtataccta tcttgcctac gtcgtgcaac aatgccccgc tagtcagggc
     7801 ttccatctgt tcgcccggca aggatatctc gctgccgatg cgcagggcga gacctgacac
     7861 ggccctcaga tgctccttga tgtaaggatc ttttgcctcc agggcctcca caagcgtcca
     7921 ggcgatcccc aagacttgat tctcgccctc gacgaacgga ccggggttct gcgctcctgc
     7981 gggtaacccg aagatccctt cagggaggcc tttggctctc tccagggcgc gcagggccgc
     8041 gtcgagaacc gggcgttcgt ccccctcctc ggcgaacacc cagcccacgg aggcggtcaa
     8101 gggcaggttg tcaggaccac cggcttcctc cgagacatgt tgcctgagcg cgagcgccgc
     8161 ctggcgggcc ctgctctcgc ccgccccgct caggacgacg aacacctcgt ccccgtagcg
     8221 gaaagcgcgc tgcctgccgt ccgcaagcct cctcccgatc ctctggaaga ggacatcctc
     8281 cgtggccaga ccatggtcac gaacgatggt cggttgcacc gccagaacag taagcggcct
     8341 gccgtaagag agttcccggc gcagaacgcg gtgcagcgac gcacggttag gcaaccctgt
     8401 gagctggtcc aacccgccag gggcgtcccc ggcgtcttcc agggcagggc ctattccttc
     8461 ggcgaggagc ttcaggacac gctcctcctc ggcgccgtac tcaccgccac cgacgatcac
     8521 cgccccccac ttctcgtctt cgcccgtgcc caggggtatg gccatgaaac cctctatccc
     8581 gtggatctcc tcctcgctgc gtacggtgtc gccctcgcgg caactttgca gcacgagacg
     8641 ctggatagag ggatgccacc aactggggca gacgtcgaag tcgccctcca gtatggtcct
     8701 gtgatagttg cctttcagcg cggcaacgtg cgcgtacggc ccgccgccta ccgtgtgggc
     8761 cgcttcgacg gtccaaccga gtatgccttc cag
//
LOCUS       DATMJA010000411         5689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_54782, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000411 DATMJA010000000
VERSION     DATMJA010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54782"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VNT12_09780"
     CDS             <1..471
                     /locus_tag="VNT12_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWI46448.1"
                     /translation="ANLAEIALPEVQPGSSIMPGKVNPVIAESATMVAAQVIGNDATI
                     ALAGQSGNFELNVMLPVIAYNLIQSIEILGSASANLADQCVSGLQATDRGPALVEQGL
                     MLATALAPEVGYDKAAELSKEAYTTGKTIREVARENTDLSEEELDRLLDARKMT"
     gene            529..1074
                     /locus_tag="VNT12_09785"
     CDS             529..1074
                     /locus_tag="VNT12_09785"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWI46449.1"
                     /translation="MDVVRQQVTADELLLMPDDGFRYELVKGELRRMNPAGNVHGRVT
                     MCVAWRLARYVEENQLGTVYAAETGFSLASDPDTVRAPDVAFVSRTRVEAIGEVEGFW
                     PEAPDLAVEVISPGDSYSEVEEKVFAWLDAGTKMVVVINPQQRSATVYKSPSDITALA
                     DVLNGGDVVPGFELALREIFE"
     gene            1230..1937
                     /locus_tag="VNT12_09790"
     CDS             1230..1937
                     /locus_tag="VNT12_09790"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46450.1"
                     /translation="MTERGETRVAVDLVVFGLVEGELSVLMGRRPHAPYEGFWALPGE
                     FRHSGQSVDELAREVLERTTGVERAWVEQLATFDRPPVVDEGGEISTPGRDPRGEIVS
                     VAYMAVIGANRVRKDYGGDLGWQAVRRLPGRVAFDHREILDYAVRRLQSKLGYSSLGF
                     ELLAEELTLPELQHVYEAVLGVALNRGNFRRKVLEAGVVEDTGKTRPARGKPAHLYRF
                     RRRAFGLFETPDGFIEG"
     gene            1948..2862
                     /locus_tag="VNT12_09795"
     CDS             1948..2862
                     /locus_tag="VNT12_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWI46451.1"
                     /translation="MFGIGYFKAQPTEFVRKVAGGRVKREGQALSFYYLKRRTSIVVV
                     PVSSADAAFVFNEQTGNYQAVTIQGQLTYRIVEPRRTIEMLNYVVDPKRRAHVSQDPE
                     RLEQRIVVAVQMETRRQVEGKSLEEVLGNAASISAEVQEEVGYRALLAPLGVELLSLN
                     FTSVSPNPEVGKALEAEYRETLLRRADEAIYARRAAAVEEEGKIKSNELEGEISLARE
                     RERLIDLEAANDRKVSQAWGERRELEAGFTARASEMELAPYRDLDPRKVLALAMRDIG
                     NNADKVGNLNITSEVLADLLQTGTNGAN"
     gene            2852..3799
                     /locus_tag="VNT12_09800"
     CDS             2852..3799
                     /locus_tag="VNT12_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46452.1"
                     /translation="MPTEWDKIVVVTRKTGLEELLERFVTREQARFYLEHSGVPFEPY
                     EEEQCAHDEAVAGLTAALPAGIRSQFVERSFLPTFAFGERDLVVTLGPDGMVVNVAKY
                     LRSGQPILAFNPDPSTVDGILILFRVGEAEAAIRAVLRGDYRVREVTMARATLGDGQN
                     LLAFNDLFIGRKTHISARYCLTHGRRSENQSSSGIIVSTGAGSTGWLSSVLNGAAGVV
                     ESFAGKEAVAPVREGYRFAWDAPRLCFSVREPFVSKASSAGIVWGNVRASEKLEVVSQ
                     MPQDGVIFSDGVEADYLAFDSGAVASISIAEEKARLVAA"
     gene            3924..5468
                     /locus_tag="VNT12_09805"
     CDS             3924..5468
                     /locus_tag="VNT12_09805"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HWI46453.1"
                     /translation="MISTPSTPIDVPEPGSAEELGRFAALQAKLGPLARRVISDPRAP
                     QTVVVVPSLTLDIEEMAKIAGAHHYEERLLCMLMLLRLPRTHVIYVTSQHIATAIVDY
                     YLHLLPGIPLRHARSRLTLLSCHDASDVPLTQKILDRPRLVERIRSAIADTEAAHMTC
                     FNSTALERSLAVRLGIPLYGNDPQLNYLGTKSGSREAFREAGIPLPDGFEHLRDGDDV
                     VEAVVELKRRKPGLRRAAVKLDEGFSGEGNAVFPYEGAPEGRDLARWVRDELPGRIKF
                     EAAGETWERYSRKFEEMGGIVECWLEGEEVRSPSVQCRINPLGEASVVSTHDQVLGGP
                     SGQIFLGSTFPADAEYSRDIKEAGDRVGEVLRQRGALGRFAIDFISVRRGESWEHAAI
                     EINLRKGGTTHPYLILRFLTDGTYDANDGLYCTPTAKPCYYYASDNLQSPTYKGLTPD
                     DLVDIAVDNGLHFDGASQQGVVFHLIGALSQYGKVGTVCIGDSHENALEYYRDTVAVL
                     DREARR"
     gene            complement(5483..>5689)
                     /locus_tag="VNT12_09810"
     CDS             complement(5483..>5689)
                     /locus_tag="VNT12_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46454.1"
                     /translation="IIIAIGLWLLAGEFLLLARLFDRIGTRLGRLGRWIKGVWRGTPA
                     AVKILVALALAATLAYGAYKLFAG"
BASE COUNT         1106 a         1694 c         1916 g          973 t
ORIGIN      
        1 gccaacctcg ccgagatagc attgccggaa gtgcaaccag ggtcctccat catgcctggc
       61 aaggtcaacc ctgtgatcgc agagagcgcc acaatggtcg ccgcgcaggt cataggcaac
      121 gacgccacca tcgccctcgc cgggcagagc ggcaacttcg agctaaacgt gatgcttcca
      181 gttatcgcgt acaacctgat ccagagcata gagattttgg gctcggcctc ggccaacctc
      241 gcagaccagt gcgtctccgg cctccaggcg acagaccgcg gccctgcact cgtcgagcag
      301 ggtctcatgc ttgccacggc cctcgcgccc gaggtcggct acgacaaagc agccgagctc
      361 tccaaagagg cctacacgac aggcaagacc atccgcgagg tggcccgcga aaacacggac
      421 ctctccgaag aagagctcga caggttactg gacgcacgca agatgacata gatccaggac
      481 ccgacggaag tgacgttact gatgttcggc tattgttaga atgagagcat ggatgtcgtc
      541 cggcaacagg ttaccgccga tgaattgtta ctcatgcccg acgatggatt ccgctacgag
      601 ctcgtaaagg gagagctgcg gcggatgaac cctgcaggaa atgtgcacgg gcgcgtgacg
      661 atgtgcgtcg cctggcgcct ggcgcggtat gtcgaagaga atcagctcgg cacagtgtac
      721 gctgccgaaa cgggattcag cctcgcaagc gatcccgaca cagtgcgcgc gccagatgtg
      781 gctttcgtga gccggacccg cgtcgaagca attggcgagg tcgaaggctt ctggcctgag
      841 gcgccggatc tagcagtcga ggtgatctcg cccggcgaca gctactcgga ggtcgaggag
      901 aaggtcttcg cctggctgga tgcaggcacg aagatggtgg tcgtaataaa cccccaacag
      961 cgttcggcca ccgtttacaa gtcaccgagc gacatcactg ccctcgccga cgttctgaat
     1021 ggcggggacg tcgtgcccgg cttcgaactc gcattacggg aaatcttcga gtagcctccg
     1081 tctctcgcat gtcagcagat gctgacttcg tcatcgcgca agatcccgca actccccata
     1141 cctcgatcca tcaagccaac acattattag atctataggc tgtttcgctc ttgacttatg
     1201 attagtttta tgattatata tgaatcataa tgacagagag aggcgagaca agggttgctg
     1261 tggacctggt ggtattcggg ctggtagagg gtgagctctc ggtgttgatg ggccggaggc
     1321 cgcacgcgcc gtacgagggg ttctgggctt tgcctgggga gttccggcat tcgggacagt
     1381 cggtggacga gttggcgcgg gaggtgctgg agcggacgac gggtgtcgag cgggcctggg
     1441 tggagcagct ggcaacgttc gaccgtccgc ccgtggtcga tgagggcgga gaaatctcga
     1501 cgccggggcg ggacccgagg ggtgagatcg tatcggttgc gtacatggct gtgatcgggg
     1561 cgaaccgggt gaggaaggat tacggtggag atctgggatg gcaggccgtg cgccggttgc
     1621 cggggagggt tgcctttgat catcgtgaga tcctggacta cgccgtgcga cggttgcaga
     1681 gcaagctcgg gtactcgtcg ctcggcttcg agttgctggc cgaggagctc acgctgcccg
     1741 aattgcagca cgtctacgag gcggttctcg gggtcgcctt gaaccgggga aacttccggc
     1801 gcaaggtact cgaagccggg gtagtggagg acacgggcaa gacgaggccg gctcgtggca
     1861 agccggcgca tctgtacagg ttcaggcggc gcgccttcgg gctcttcgag acgccggacg
     1921 ggtttatcga gggatagggg cgaggatatg ttcggaatcg ggtacttcaa ggcgcaaccc
     1981 acggagttcg tcaggaaggt ggccggtgga cgggtgaaga gggaggggca ggcactctcc
     2041 ttctactacc tcaagcgccg gacgagcatc gtggtagtcc ccgtctccag tgctgacgcg
     2101 gcgttcgtgt tcaacgagca gaccggaaac taccaggcgg tcaccataca gggccagctc
     2161 acgtaccgca tcgtcgagcc gcgccggacc atagagatgc tcaactacgt ggtcgacccg
     2221 aagcggcggg cgcacgtctc ccaggacccg gagcgcctag agcagaggat cgtcgtcgcc
     2281 gtccagatgg agacccgtcg tcaggtagaa ggtaagtcgc tcgaagaggt gctagggaac
     2341 gctgcgagca tctccgctga ggtccaggag gaggtgggat accgtgccct actggcgccg
     2401 ctcggggtgg agctactcag cctgaacttc acctccgtca gccccaaccc cgaggtcggg
     2461 aaggctctgg aggcggagta cagggagacc ctgctgaggc gggctgacga ggcgatctac
     2521 gcgcggaggg ccgctgcggt cgaagaggaa ggcaagatca agtcgaacga gctggagggt
     2581 gagatctccc tcgcccgcga acgcgagcga ctcatcgacc tggaagccgc caacgaccgc
     2641 aaggtgtctc aggcgtgggg cgagcggagg gagctagagg ctggcttcac ggcgcgggca
     2701 agcgagatgg agctcgcccc ttaccgcgac ctcgatccca ggaaggtcct cgccctcgcc
     2761 atgcgcgaca tcggcaacaa cgcggacaag gtcggcaacc tcaacatcac ctccgaggta
     2821 ctcgcggatc tcctgcagac ggggacgaac ggtgccaact gagtgggaca agatcgtggt
     2881 cgtcaccagg aagacgggac tggaggagct tctcgaacgc ttcgtaacgc gggagcaggc
     2941 ccgtttctac ctggagcact caggcgtgcc ctttgaacct tatgaggagg agcagtgcgc
     3001 ccacgacgag gccgtcgccg gtctcacggc ggcgctcccg gctggcattc gcagccagtt
     3061 cgtcgagcgc tcgtttcttc ccacgttcgc gttcggggag cgggacctcg tcgtgacact
     3121 cgggcctgac ggcatggtcg tcaacgtcgc caaatatctg cgcagcgggc agccgatcct
     3181 ggccttcaac ccagaccctt cgacagtaga cggtatcctc atactgttcc gtgtggggga
     3241 ggccgaggcg gcgatccggg ccgtcctgcg cggagattac agggtacgcg aggtgaccat
     3301 ggcccgcgcc acgctcggcg acggccagaa cctgctcgcc ttcaacgacc tgtttatcgg
     3361 gaggaagacc cacatctcgg ctcgctactg ccttacgcac gggcggcgct ccgagaacca
     3421 gtcttcgagc ggcatcatag tctcgacggg cgcggggtcc accggctggc taagctccgt
     3481 cctcaacggc gcggcggggg tcgtcgagtc cttcgcaggg aaggaggccg tggcgccggt
     3541 ccgcgaggga taccgtttcg cctgggacgc gccgaggctg tgcttcagcg tcagagagcc
     3601 gttcgtcagc aaggcgtcgt cggcagggat agtgtggggc aacgtcaggg ccagcgagaa
     3661 gctggaggtc gtcagccaga tgccgcagga cggtgtgatc ttcagcgacg gcgtcgaggc
     3721 cgattacctg gctttcgact ccggcgccgt cgccagcatc tccatcgccg aagagaaggc
     3781 aaggctcgtc gccgcataga ggcggcgagc gtcgcaggca gtagtaccga gcgattgggc
     3841 gagaaattcg caacggcgga gagcctctgc gagacacggg aggctcgcgt ataattttcg
     3901 cgcggggaac aggagtgacg agtatgatct ccacaccatc gacgccgatt gatgtgcctg
     3961 agccgggttc agcggaggag ctcgggcgct tcgcagcttt gcaggcgaag ctcggaccgc
     4021 tggcgcggcg ggtgatctct gatcctcgcg ccccccagac ggtcgtagtg gtgcccagcc
     4081 tcacgctcga catcgaggag atggcgaaga tcgccggagc gcaccactac gaggaacggc
     4141 tgctctgcat gctgatgctc ttgcgactgc cccgcaccca cgtcatctac gtcacgagcc
     4201 agcacatcgc gacggccata gtggactatt atctgcatct cctgccgggc atcccattgc
     4261 gccacgcccg cagccggctg acgctcctct cctgccacga cgcctccgac gtgccgctga
     4321 cgcagaagat actcgaccgg ccgcgtctgg tggagcggat acgctctgcg atagcagaca
     4381 cggaggcggc gcacatgacc tgcttcaaca gcacggcgct ggaacggagc ctcgccgttc
     4441 ggcttggcat cccgctctac ggcaatgatc cgcaactcaa ctacctcggg accaagagcg
     4501 gcagccgcga ggcgtttagg gaggccggga tcccgcttcc cgacgggttc gagcacctgc
     4561 gcgacgggga cgacgtggtc gaggccgtcg tcgagctcaa gcgccgcaag cccgggctgc
     4621 gaagggcggc cgtaaagcta gacgagggtt tctcgggtga ggggaatgcc gtcttcccct
     4681 acgaaggcgc gcctgagggg agggatcttg cacgctgggt cagggacgag ttgccgggac
     4741 gcatcaagtt cgaggcagcg ggagagacct gggagcgtta cagtcggaag ttcgaggaga
     4801 tgggcggcat cgtggagtgc tggctggagg gagaagaggt gcgctcgcct tccgtgcagt
     4861 gccgcatcaa tccgctcggg gaggcaagcg ttgtctccac ccacgaccaa gtcctgggag
     4921 ggccctcggg ccagatcttc ctcggcagca cgttccccgc cgatgccgag tacagccgcg
     4981 acatcaagga ggctggagac agggtaggag aggtgctaag gcaacgcggg gccctgggaa
     5041 ggttcgccat cgactttatc tccgtcaggc gcggcgagag ctgggagcac gcggctatcg
     5101 agatcaacct ccgcaagggc ggcaccacgc atccgtacct gatcctgcgt ttcctgacgg
     5161 atggtaccta cgacgcgaat gacggattgt actgcacgcc gacggcgaag ccctgctact
     5221 actacgcttc tgataacctc cagagtccca catacaaggg cctgaccccc gacgatctgg
     5281 tggatatagc cgttgacaac ggcctccact tcgacggcgc ctcccagcag ggggtggttt
     5341 tccacctcat cggggcgctc tcccagtacg gcaaggtcgg caccgtctgc atcggcgata
     5401 gtcacgagaa cgccctggag tactatcggg ataccgttgc ggtcttggac cgcgaggcgc
     5461 gacggtaacg ggtcggaccg gtctacccgg cgaacagctt gtaggctccg tatgccaaag
     5521 tggcggcgag ggcaagtgcg accaggatct tcaccgcggc gggcgtgcct ctccacacgc
     5581 ctttgatcca ccggcctagt cttcccagcc tcgtacctat cctgtcgaag aggcgggcga
     5641 ggagtaggaa ctcgccggcg agcagccaga gcccgatggc gatgatgat
//
LOCUS       DATMJA010000412         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_113766, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000412 DATMJA010000000
VERSION     DATMJA010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113766"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VNT12_09815"
     CDS             complement(<1..1238)
                     /locus_tag="VNT12_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWI46455.1"
                     /translation="MDESVSHATEHPDLKRSIGLYMLLFFVLGDIVGAGIYALVGEVG
                     AIVGGAIWTAFLAAFVLAIFTAASYAELVTKYPRAGGSATYVNNAFKNPFVSFMVAFA
                     VMASGITSACTLTLAFSGDYLSQFISVPIVAAALVFIVVVGLINFYGISESVKINVVL
                     TSVEIFGLLLIILIGVLALLAGEGDPGRAFEFKGDASVLGAILAGTVLAFYALIGFDD
                     SVNVAEETHNPSRNYPRAIFGALFIAGIVYLLVTFTASMVVPTGELAGSSGPLLEVVD
                     RGTLPVPAKLFAAIALLAVSNGALINMIMASRIVYGMSDQGVVPSVFSRVHEGRRTPW
                     IAILFTVSIALVLLAVLGGNPDALDTLGSTTVVLLLLAFIMVNVSVMVLRGDRVGHEH
                     FRTPTIFPILGIVVSAALLI"
     gene            1424..1810
                     /locus_tag="VNT12_09820"
     CDS             1424..1810
                     /locus_tag="VNT12_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI46456.1"
                     /translation="MEIRLDLVGIVVRDMRASLKFYRRLGLDIPGGAEDEPHAEATTP
                     SGLRVAWDTADLIQEFDPTWTEPSGGHRMALAFLCSTPGEVDATYERLAALGHGYKEP
                     WDAFWGQRYAIVKDPDGNHIDLFAPL"
     gene            complement(1817..2905)
                     /locus_tag="VNT12_09825"
     CDS             complement(1817..2905)
                     /locus_tag="VNT12_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWI46457.1"
                     /translation="MADKIVFTRGVPPPEAFPTEELGACVDAAVRENPDVVLQYGQQP
                     GYAPLREELAGEYGVSPQQILIGNGSLQLQDLVSSYLIRPGAAVYTEQPSYDRAIKTF
                     RRRGARTVGISLEEDGISVERLEAALRREVPAFLYLVPDFQNPAGATLSLEKRRRVVE
                     LADLYGFWLIEDIPYRKLRYEGDDVPLLREIDPSRVITMSSFSKLLSPGIRVGFMISP
                     EDLIRGVTALGEETYLSPVLPTQAAVAEYLRRKLLGPNVERLKDLYTPRWKAMTGAVR
                     RELPGAQAFIPSGGFFVSVMLPEDANIENLVGRAEDIGLVLTPGAAFFADPEEGGEAG
                     GDRFVRLPFCAVTPEQIEEGVRRLASLL"
BASE COUNT          616 a          992 c          923 g          533 t
ORIGIN      
        1 tatatcagga gagccgccga gactacgatg ccaaggatgg ggaagatggt cggggtgcgg
       61 aagtgttcgt ggccgaccct gtcaccccgc aacaccatta cggatacgtt caccatgatg
      121 aacgcgagca agaggagcac caccgtggtg ctgccaaggg tatccagagc atcggggttg
      181 ccgccgagca cggcgagcaa tacgagggcg atgctgacgg tgaacagtat ggcgatccac
      241 ggcgtgcgcc gtccctcatg gacccgtgag aacaccgagg gcaccacccc ctggtcgctc
      301 atgccgtaga cgatccggga ggccatgatc atgttgatga gcgccccgtt ggagaccgcg
      361 aggagcgcga tggccgcgaa cagcttcgcc gggaccggaa gcgtacctct gtccaccacc
      421 tccagcaacg gtccactcga cccggcgagc tcacccgtcg gcacgaccat cgaggcggtg
      481 aaggtaacga gcaggtagac gattccagct ataaacaggg ccccgaatat ggcgcgggga
      541 tagttccggc tcgggttgtg ggtctcctcg gcgacgttca ccgagtcgtc gaagccgata
      601 agggcataga acgccagcac cgtgccggcc aggatcgctc ccagcacact cgcgtccccc
      661 ttgaactcga aggcgcggcc ggggtctccc tccccggcca ggagggccaa caccccgatc
      721 aggatgatca ggagcagccc gaagatctcg acggatgtca ggaccacgtt gatcttcacc
      781 gactcggaga taccgtagaa gttgatgagg ccgaccacca ctatgaagac cagcgcggcg
      841 gcgacgatcg gcacgctgat gaactggctg aggtagtctc cgctgaacgc gagggtcagc
      901 gtgcaggccg aggtaatccc cgacgccatc accgcgaacg cgaccatgaa gctgacgaac
      961 gggttcttga aggcgttgtt gacataggtg gccgatccgc ccgcccgagg atacttggtg
     1021 actagctcgg cataggaggc ggcggtgaag atagcgagca cgaacgccgc cagaaacgcg
     1081 gtccagatag caccgccgac tatcgccccg acctcgccca caagtgcgta gattccggcc
     1141 ccaacgatgt ctccgaggac gaaaaacaga agcatataca gaccgatgct gcgctttaga
     1201 tccgggtgct cggtagcgtg agatacgctc tcgtccatcc gtcccacctc cctcgtcgca
     1261 ggccaataca ggcctttctt tacccttccg tccccatgat accctagtcg ccccggatct
     1321 tcatggccag tccatgcacg tcccctccgt ggtcttgcga ggaattgccc gttggtagcg
     1381 cccctcccct ggtgtatgtt taagaagacc gaggaggaag ggaatggaga tccgactgga
     1441 cctcgtgggt atcgtcgtgc gcgacatgcg tgcctccctg aaattctacc gaaggctcgg
     1501 tctggacatc ccagggggcg ccgaggacga accccacgcc gaggcgacaa caccatcagg
     1561 cctgcgcgtc gcgtgggata ccgccgacct tatacaagag ttcgacccga cctggacgga
     1621 gccctcgggc gggcaccgta tggccctggc tttcttgtgt tcgaccccag gagaggtcga
     1681 cgcgacctac gaaagactgg ccgcgcttgg tcacggatac aaggagccat gggacgcgtt
     1741 ctggggacaa aggtacgcca tcgtcaaaga ccccgacggc aaccacatcg acctcttcgc
     1801 accgctctag atatcttcag agcaggcttg ccaggcgacg cacgccctct tctatctgtt
     1861 ccggggtcac ggcgcagaaa ggcaggcgta cgaacctgtc gccgcccgcc tcacctcctt
     1921 cctccggatc tgcgaagaat gccgcgcctg gcgtgagcac gagcccgatg tcctctgccc
     1981 gccccacgag gttctcgatg ttcgcgtcct cgggaagcat cacgctcacg aagaacccgc
     2041 cggaggggat gaaggcctgg gcgcctggca gctcgcgacg taccgcgcct gtcatcgcct
     2101 tccagcgggg tgtgtagagg tcctttagcc gctctacatt cggaccgagg agtttgcggc
     2161 gcaggtactc ggcgacggct gcttgtgtag ggagtactgg cgaaagatat gtctcctccc
     2221 cgagcgccgt caccccacgt ataaggtcct cgggggagat catgaacccc actcttatgc
     2281 ctgggctgag gagcttgctg aacgagctca tcgtgatcac gcgcgagggg tcgatctcac
     2341 gcagtagcgg aacgtcatcg ccctcgtaac gcagcttacg gtaagggatg tcttcgatca
     2401 accagaagcc atagaggtcc gccaattcca cgacccgccg ccgcttctcc agcgagagcg
     2461 tcgcgcctgc ggggttctgg aagtcaggca cgaggtacag gaaggcaggc acctcgcgtc
     2521 tgagggccgc ctcgagccgc tcgacgctta tgccgtcttc ttccagcgaa atccccaccg
     2581 tacgtgcccc gcggcgtctg aaggtcttga tcgcacggtc gtagctaggc tgttccgtat
     2641 agactgccgc acccggcctg atgagatagg acgagacgag gtcctgcaat tgcagcgatc
     2701 cattgccgat caggatctgc tgcggcgaga ccccgtactc accggctaat tcctcgcgca
     2761 gcggcgcata gccgggctgt tggccgtact gcaacacgac atcgggattc tctctgactg
     2821 ccgcgtcgac acacgccccc agttcttcgg tcgggaacgc ctctggcggt gggacgccgc
     2881 gcgtaaagac tatcttatct gccatgttca caagcctccg cttctagatc gggaagtctg
     2941 catcatcgcc cggcccgcac cccagcgcaa ccccctgccc cccgtgcacg tcatgccctg
     3001 gtggtctcca ggtcagtgcc cacagaaatc ctgaatccgg agagagccgc aaggcgaccg
     3061 tcct
//
LOCUS       DATMJA010000413         4508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_113771, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000413 DATMJA010000000
VERSION     DATMJA010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113771"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..1187)
                     /locus_tag="VNT12_09830"
     CDS             complement(303..1187)
                     /locus_tag="VNT12_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46458.1"
                     /translation="MASQRKRNRRTKAEVEAVRRAAREELAAGHPMTLRQVHYRLVSR
                     DDVVHPNTISAYNTLGGWLRDDRLAGDVPWEWMEDRLRVAKGWQMWTDPAEFLGDVQS
                     DYYRNPWQDQSHYVEVWCEKDALSGIFSDALSRYRVTLNIGRGYDGWSSIKRAADRYY
                     RRKDEGIDTTVLYFGDFDPSGEDMHRSLIERFATLGVYPEIPKAALTPEDARRLPGDV
                     TKADDTRAAAFVAKYGDLAVELDALPVGQLRSRIQQGVEEWMDMEALSENARIERAQR
                     EKLRDSIGRIFEDNEQDS"
     gene            1254..1451
                     /locus_tag="VNT12_09835"
     CDS             1254..1451
                     /locus_tag="VNT12_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI46459.1"
                     /translation="MLTSWIFQTVWNRHPGGSFAWVVIVTIIVSRATIATIESGKRTR
                     AHPATAKRLARVLKVRPEQQG"
     gene            complement(1448..3403)
                     /locus_tag="VNT12_09840"
     CDS             complement(1448..3403)
                     /locus_tag="VNT12_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI46460.1"
                     /translation="MSTGDYSDHQAFVDEIGSMIMEPKPEKAQANGHGKASSASATLP
                     TDEAVLRKCRDAGNAQKFIALYDFGDLSGYGGDDSRADLALARILGFWTQDAWQVERL
                     ISGSALGQREKWRRRRDYRERTIRRALDGLTETYQWPTDQGVTGVTDYIGSDSSDTLE
                     VVRFARLDPPPPREFVMPGLVPRSYPTTLFGWGGTAKSLLAALLAMSVAGGRPKFLGR
                     DVTVHGPTLFVDFELDGVEQHRRILQLAAGLKMEVPDDFLYVSALGAHTPDAIKFALA
                     LCKKHDVVLVVLDSLGKALVGDMLAAKDVIGFHNTFIAPFQNQGVTPVLVDHQARQQP
                     GEGYQSKGAYGSAYKEHLSRSLIQVEAGDRSAEQGVLNVRLRHKKSNFAPLEEPFDVA
                     LTFSDDVIVATTRELTAQDKAQEQTLNGTDRVLAALEDGPLFPDEIADVTGLKLGTVK
                     NKLVALKKAGRVVSTGQIRNQSEQVSLVSLIYKGSDTYESVANFFQDPPDWLPGQLEK
                     YRERPELHFRSLCMTVAAKVLDNGDRWEEVKEEVERELAKLEAGGVTRVTNIDEGEPY
                     EVYVGRGKRRAGLKKSKWHNPYVVDKHGTRKECVEWYERDFRAGDLGVGWDDLPEIRG
                     KVLGCHCKPKPCHGDVLVRLVEEGTQG"
     gene            complement(3400..3888)
                     /locus_tag="VNT12_09845"
     CDS             complement(3400..3888)
                     /locus_tag="VNT12_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46461.1"
                     /translation="MITETTGLITLIPENIPEELTERPQWVTWCLVERGKRLTKVPFN
                     ARTGEPASCVDLLSWSDFSTAGAAYDAGGYDGVGFVLSSGDPFTALDLDDCRSAAGVI
                     APWAQEILDRVPEDAYREVSVSGAGVHVILRAKVRAGGMRRGCVELYSRERYIALTGV
                     RL"
     gene            complement(3951..>4508)
                     /locus_tag="VNT12_09850"
     CDS             complement(3951..>4508)
                     /locus_tag="VNT12_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46462.1"
                     /translation="GIATTKSFADINEGTQVEVLIADATYDEAGRFGPEIEFALDVLK
                     PSAHAGETIMSTFGLSQPRLSKVRDLRDDGLDDETIAKVLEKKGFQFEDIDDPETPKL
                     GGALLRIVKACYNADPRALKKLLEECLTFDDLANALIGKRFVATTRKDKNDYTRLDGK
                     GEFYRVMAPAQAEAEEDEDFEKIPF"
BASE COUNT          813 a         1433 c         1298 g          964 t
ORIGIN      
        1 ctccctacaa ttgaagcctg aggaaggata attcgacata ccgccattcc ctggctgcta
       61 ttcacccaac ctcgtagaat aagaattctc ggaagttcat atcggcgatc ctgcatatag
      121 ggccctctga gaggcgaaat aaggccttct gaggccctgc gcgccgcgtg gacaacctct
      181 ccaacctgtt cgacgatcag gaggtgcctc ctgttcatcc tgctcgacga acaaggggta
      241 atctccgctc caggcttgac gttgatggcg tccacctcgc actggacccc ggcccggtgg
      301 ctttagctgt cttgttcgtt atcctcgaag atgcgcccta tactgtctct gagtttctcc
      361 cgttgggcgc gctcgatgcg ggcattctcg gaaagtgcct ccatatccat ccactcttcg
      421 acgccctgct ggatgcggct ccggagctgc ccaacgggca gcgcatccag ctccacggcc
      481 aggtcgccgt acttcgcgac gaaggcagca gcgcgggtat catctgcctt cgtcacatca
      541 cctggcaacc tccgggcatc ctcgggagtg agcgcggcct tgggtatctc cgggtagacg
      601 cccaaagtgg caaagcgctc aatcagcgag cggtgcatgt cctcacccga ggggtcgaag
      661 tcgccgaagt acagcaccgt ggtatctatc ccctcatcct tgcgacggta gtagcggtcg
      721 gcggcccgtt tgatagagga ccatccatcg tacccacgcc cgatgttgag agtaacgcgg
      781 tagcgtgaga gcgcatcgga gaagatgcca gatagcgcat ccttctcaca ccagacctcg
      841 acgtaatgac tctggtcctg ccaggggttg cggtagtaat cgctctgcac gtcgccaagg
      901 aactcggccg ggtctgtcca catctgccag cctttcgcga cacgtagcct atcctccatc
      961 cactcccacg ggacatcccc agccaaccta tcgtccctaa gccatcctcc caaggtgttg
     1021 tatgccgaga tcgtgttggg atggaccaca tcgtcccgcg agaccaggcg gtagtgcacc
     1081 tgcctgagcg tcatcggatg cccggcggcc aactcttcgc gggcagctcg tcgtacagcc
     1141 tcgacctcgg ccttggtccg cctgttgcgc tttctctggc ttgccaacta aatccctaag
     1201 agtcaagggc cggggcaccc gactaggata ccccggccct gaaggtagct gaagtgctga
     1261 cgagctggat attccagacc gtctggaatc ggcaccctgg tggttccttc gcttgggtcg
     1321 tgatcgtgac gatcatcgtg agtcgggcga cgatcgcgac catcgagtcc ggcaagcgca
     1381 ccagggcaca ccccgcgacg gcgaagaggc tcgccagggt gctgaaggtc aggccggagc
     1441 agcagggcta gccctgcgtg ccctcctcga ccagcctgac gagcacgtct ccgtggcagg
     1501 gcttcggctt gcagtgacac cccaggacct tgcccctgat ctcgggcagg tcgtcccacc
     1561 cgacccccag gtccccggcg cggaagtcgc gctcatacca ctcgacgcac tccttgcgcg
     1621 tcccgtgctt atccacaaca taggggttgt gccacttgct cttcttcagg cccgccctgc
     1681 gcttgccgcg acctacgtag acttcgtagg gctcgccttc gtcgatgttt gtgactctgg
     1741 taactcctcc tgcttcgagt tttgctagct ccctctcgac ctcttcttta acctcttccc
     1801 agcggtctcc gttatctagg accttagcag cgacggtcat acacagtgat ctgaaatgca
     1861 gctcggggcg ctcgcggtac ttctcgagct gtccaggcag ccagtcggga gggtcttgga
     1921 agaagtttgc gacgctctcg taggtgtcac tgcccttata tatgagtgac accagggaca
     1981 cctgttcgga ttggttgcgt atttgcccgg tcgacacgac gcggccggct ttcttgagtg
     2041 cgacgagctt gtttttcacc gtgcccagtt tgagtccggt aacgtcggcg atctcgtcgg
     2101 ggaatagggg gccgtcttcg agggccgcga gcacccgatc cgtaccgttc agggtctgct
     2161 cctgtgcctt atcctgcgca gtaagctccc gtgtggtggc gacgatcacg tcgtcggaga
     2221 acgttagcgc cacgtcgaag ggctcttcga ggggggcgaa gtttgatttc ttgtgccgga
     2281 gacgcacatt tagcacgccc tgctctgcgc tcctgtcgcc agcctcgacc tgaatcaggg
     2341 accggctgag atgctctttg taggcgcttc cgtacgcccc cttactctgg tagccctctc
     2401 ccggctgctg acgcgcctgg tggtcgacga gaacaggagt cactccctgg ttctgaaatg
     2461 gcgcgatgaa ggtattgtga aagccgataa catccttcgc cgccagcatg tcgccgacca
     2521 gggcttttcc gaggctgtcg agcacgacga gcacgacgtc gtgcttttta cacagtgcca
     2581 aggcgaactt gatggcgtcg ggtgtgtggg cgcccaacgc actcacgtac agaaagtcgt
     2641 cgggcacttc catctttaaa cccgccgcga gctgcaggat tctcctgtgc tgttcgactc
     2701 catccaactc gaaatcgacg aataacgtgg ggccgtgtac tgttacgtcc cgccctagga
     2761 acttcggccg gccgccggcg acgctcatcg cgagtagggc ggcgagcaaa ctcttcgccg
     2821 tcccgcccca gccgaagagt gtcgtggggt agctgcgggg cacgaggccc ggcatgacga
     2881 actcacgcgg cggcggcgga tccaaccgcg caaaccgcac cacttccaag gtgtcactgc
     2941 tgtcactccc tatatagtca gttacaccag tgacaccttg atccgtgggc cactgatatg
     3001 tctctgtgag accgtcgaga gcccgtcgga tcgtgcgctc gcggtagtcg cggcgccggc
     3061 gccatttctc acgctgcccg agagcggagc ccgatatcag ccgctcgacc tgccaagcat
     3121 cctgtgtcca aaatcctagg atccgggcca gtgcgagatc cgcccgactg tcgtcgccgc
     3181 cgtaaccgct gagatctccg aagtcataga gcgcgatgaa tttctgagcg tttccagcgt
     3241 cccgacactt ccttagtacg gcctcgtcag tggggagcgt cgccgaagcc gacgacgctt
     3301 tcccatgtcc gtttgcctgt gccttctccg gtttgggctc catgatcatg gagccgatct
     3361 cgtcgacgaa agcctgatga tcgctgtaat caccagtgct cacaaacgca cccccgtaag
     3421 ggcgatgtat cgctcgcggc tgtagagctc gacgcacccg cgtcgcatgc cgccggccct
     3481 caccttcgcc cgcaaaatca cgtgaacacc ggctccgcta accgacactt cgcggtaagc
     3541 atcctccggc acgcggtcga gaatctcctg tgcccacggc gcgattactc cggctgcgct
     3601 gcgacaatcg tcgaggtcga gcgccgtgaa tggatcgccc gaactgagga cgaagccgac
     3661 gccatcgtag ccgccggcgt catacgcggc gccggcagtc gagaagtccg accagctgag
     3721 caggtcgacg caagatgccg gctcgccagt ccgcgcgttg aacggtactt tcgtcagacg
     3781 cttgccgcgc tcgacgaggc accaggtaac ccactgaggg cgctcggtga gctcctcggg
     3841 gatgttctcg gggatcaggg tgataagccc tgttgtttcg gtgatcatcg ctgtatactc
     3901 ctttacagat gagtgcgagg gccccaatct cggggccctc gtcgtttact ctagaacggg
     3961 attttttcga agtcctcgtc ctcttcggcc tcggcctggg cgggggccat tacgcgataa
     4021 aactctccct tcccgtctag acgtgtgtag tcgtttttat ccttgcgggt cgtggcaacg
     4081 aaacgcttcc cgataagcgc attggccaag tcgtcgaacg tgaggcactc ctcaagaagc
     4141 ttcttgagtg cgcgtgggtc ggcgttgtag caggccttga cgatgcgcaa gagcgcgcca
     4201 ccaagctttg gcgtctcggg atcgtcgatg tcctcgaact ggaagccctt cttttccagc
     4261 actttggcga tggtttcatc gtcgagcccg tcgtccctga gatcacgaac ctttgaaagc
     4321 cgcggctgag agagaccgaa agtactcatg atggtttctc cagcatgcgc ggatggcttg
     4381 agcacatcta gcgcaaactc gatttcggga ccgaagcggc cagcctcgtc gtaggtggcg
     4441 tcggcgataa gcacctccac ctgggttcct tcattgatgt ccgcgaagga tttggttgtg
     4501 gctatacc
//
LOCUS       DATMJA010000414         2551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_351312, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000414 DATMJA010000000
VERSION     DATMJA010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351312"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /gene="sufD"
                     /locus_tag="VNT12_09855"
     CDS             <1..837
                     /gene="sufD"
                     /locus_tag="VNT12_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HWI46463.1"
                     /translation="EVPIQSYRWLDAISATMPRTLVIVEENASVTYIDEYASGGTEED
                     EPALSNGAVELYVGQGANLRYVSLQNWGRNVLHFNTIRSEAGKDATINSLVVALGSEL
                     SRTNVDAGLAEAGSDSEMLGLYFADSNQVLDHHTLQDHLAPNAHSDLLYKGALRDESL
                     AVFSGLIRVEPGAQKTDAYQTNRNLILGTDDAMAVSLPRLEIQADDVKCSHGSTTGQV
                     DDVELFYLMSRGIPRREAEKLVVFGFFGEVTSRIPLKGLKDKLDRAIEGKIGLGFEER
                     VA"
     gene            843..1196
                     /locus_tag="VNT12_09860"
     CDS             843..1196
                     /locus_tag="VNT12_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HWI46464.1"
                     /translation="MAEFHKLATVDEVGSGEIKQYLVEDRFVALCNVEGEFHAFEDVC
                     THQFTHLSEGEFTGSEVKCPLHGAKFDVKTGAAKSLPAVKPVPKHEVKVENGNVYVAL
                     NPKRVKVGGRRGRRR"
     gene            1406..1813
                     /locus_tag="VNT12_09865"
     CDS             1406..1813
                     /locus_tag="VNT12_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWI46465.1"
                     /translation="MQSFYREILLDHFEHPRNRRELEDANIHEHLDNPLCGDMVTVYA
                     KVHDGVFEEVSFTGRGCSISQASASMLTEQLTGKSREEAEEKIEAFLEMMRTEEREEL
                     GELAALKGVVQTPNRIRCATLAWDAMRRALPEP"
     gene            1913..>2551
                     /locus_tag="VNT12_09870"
     CDS             1913..>2551
                     /locus_tag="VNT12_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWI46466.1"
                     /translation="MEIEERRSVGRPAPGGFQHERLDRALARASDAPLREGNRLELLR
                     NGPDTYEDWLAAISGAERWVHLDNYIFSDDEVGERFARALIQKAEEGVSVRVLYDWFG
                     CLDVDRSFWRRMREAGVVVRAVNPPTLGGPLGAIRRDHRKLLAVDGEYASTGGVCISD
                     GWLVTSPETGLPYRDTAVGIRGPAVAEVERAFAGLWKVAGGSLPETERPDSER"
BASE COUNT          506 a          800 c          843 g          402 t
ORIGIN      
        1 gaggtgccga tccagagcta ccgttggctc gacgcgatca gcgccacgat gccgcgcacg
       61 ctcgtcatcg tcgaagagaa tgcgagcgtc acctacatag acgaatatgc ttcggggggg
      121 accgaggagg acgagcctgc cctgagcaac ggcgcggtcg agctctacgt cggccagggc
      181 gcgaatctgc gctacgtctc cttgcagaac tggggccgca acgtgcttca tttcaacacc
      241 atccgcagcg aggcaggcaa ggacgccacc ataaacagcc tcgtggtcgc cctgggttcc
      301 gagctcagcc gcaccaacgt ggacgcgggt ctagccgaag ctggcagtga ctcggagatg
      361 ctcggcctgt acttcgccga ctccaaccag gtcctcgacc accacacttt gcaggaccac
      421 ctcgccccga acgcgcactc tgacctgctg tacaagggcg ccttgcgcga cgagtccctc
      481 gccgtgttca gcggcctgat ccgggtcgag cccggcgcgc agaagacaga cgcgtaccag
      541 accaaccgca acctcatcct cggtaccgac gacgcgatgg cagtctccct gccacggctg
      601 gagatacagg ccgacgacgt caagtgctcc cacggctcga ctaccggcca ggtggacgat
      661 gtggagctct tctatctcat gagccgcggg atcccccgcc gcgaggccga gaagctcgta
      721 gtgttcggct tcttcgggga ggtcacgagc aggatccccc tgaagggcct gaaagacaag
      781 ctcgaccggg ccatagaggg taagatcggg ctcggcttcg aggagcgtgt ggcatagcga
      841 acatggccga gttccacaag ctggccaccg tggacgaggt aggctcgggg gagatcaaac
      901 agtacctcgt tgaggaccgc ttcgtggccc tgtgcaacgt ggagggcgag ttccatgcct
      961 tcgaggacgt ctgtacccac cagttcacgc acctgagcga gggcgagttt acaggttcgg
     1021 aagtgaagtg tcccctccac ggcgcgaagt tcgacgtgaa gaccggcgcg gcgaagagcc
     1081 tgcccgccgt gaaacccgtc ccgaagcacg aagtgaaggt agagaacgga aacgtctacg
     1141 tcgcgctcaa ccccaagcgg gtcaaggtcg gaggcagaag aggccgcagg aggtagagac
     1201 ccccccttgc cgaagcggcc ggaacgggtg cacgctgggg ttcgccgcac cctggtgtgt
     1261 gatccccgag gacgagaggc gcttcgccct ttggccagaa gagtccatag cagaggggca
     1321 ccgccgccac gagctctagc ctgacctcgg aaatgcagga acacacgaag tcccgcacgc
     1381 tcctgataca atttcctgca aggtgatgca gagcttctac agagagatcc tcctcgacca
     1441 cttcgagcat ccgcgcaaca ggcgcgagct ggaggatgcc aacattcacg aacacctgga
     1501 taaccctctc tgcggcgaca tggtgactgt ctacgcgaag gtgcatgatg gggtcttcga
     1561 agaggtgagc tttaccggac gcggatgctc catctcccag gcgtcggcct cgatgctcac
     1621 ggagcaactc acgggcaaga gccgcgagga ggcggaggag aagatagaag ccttcctcga
     1681 gatgatgcgc acggaggaaa gggaagagct cggcgagctc gccgccctca agggcgtcgt
     1741 ccagaccccc aaccgcatca ggtgcgccac cctcgcctgg gatgctatga ggcgcgctct
     1801 ccctgagcca taagtaccgc cggcaccgtg caccgcccgc aggacctctt tcgtcgacgt
     1861 gagcgtagca accgggtaga gtagcactat acctttcgcg aggaggccgg ggttggagat
     1921 agaagagcga agatctgtgg gaagaccggc gccgggcggg ttccagcacg agagactgga
     1981 ccgcgccctg gcgcgtgcca gcgacgcgcc gctccgggag ggcaaccggt tggagcttct
     2041 caggaacggc cccgacactt atgaggactg gctcgcggcc atctccgggg cggagcgctg
     2101 ggtgcatctg gacaactaca tcttcagcga cgacgaagtc ggggagaggt tcgcgagggc
     2161 tttgatccag aaggctgagg agggcgttag cgtccgtgtg ctctacgatt ggttcggctg
     2221 cctcgacgtg gaccgctcct tctggcggcg gatgagggag gccggcgttg ttgtcagggc
     2281 ggtcaacccg ccgacgcttg gtgggccgct cggcgcgatc cggcgcgacc accgcaagct
     2341 acttgccgta gatggcgagt acgcctcgac gggcggcgtg tgcatctccg acggctggct
     2401 cgtgacctcc cccgagacgg ggctccccta cagggacacg gccgtcggca tccgcggccc
     2461 ggctgtggcc gaggtggagc gggcctttgc aggactgtgg aaagtcgccg gtggctccct
     2521 ccccgaaacc gaacgtcccg actccgagag a
//
LOCUS       DATMJA010000415         3097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_173863, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000415 DATMJA010000000
VERSION     DATMJA010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173863"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VNT12_09875"
     CDS             complement(<1..411)
                     /locus_tag="VNT12_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWI46467.1"
                     /translation="MKGAVARGVENNGLRSGFIAVVGRPNVGKSTLVNRLVGQKVSIT
                     SSRPQTTRSPIRGVRNGPGYQAIFVDTPGSQKARDTLRARMQEQVVDSLSESDVILLL
                     FDALQASEGLGSSDRYVARLVAGSGTPAIVSINKT"
     gene            complement(408..797)
                     /locus_tag="VNT12_09880"
     CDS             complement(408..797)
                     /locus_tag="VNT12_09880"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWI46468.1"
                     /translation="MDPEAVMEAARVAANRAYAPYSNIHVGAAILTGGGTIHSGANVE
                     NASYGLSICAERNAVTMMATADPEDREIELVAVFSPNTSPCFPCGACRQVLREFGCKE
                     VVVETGRGLERYPFDQILPNSFSPEDL"
     gene            complement(809..1552)
                     /locus_tag="VNT12_09885"
     CDS             complement(809..1552)
                     /locus_tag="VNT12_09885"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HWI46469.1"
                     /translation="MARKVEQPSKGQQGVARSFEHAYRGMIYAVRTQRNMRFHVVVAV
                     LVLVASLLVGVRKLELAVLVLTILLVFVTELFNTAMEFVVDLATREYHPLAKLAKDVS
                     AGAVLVSTVGAVLVGYLVLADDLGPLSLATLQSIRRQPAHLTLVTLGVIVLIVLLGKA
                     LTHSSNSFAGGMPSGHAAVAFAGWVAASFIAVQGGAGSYAGIISVISLLMALLVCQSR
                     VESGIHSVYQVIMGAVIGVLVTTAAFQLL"
     gene            complement(1536..1946)
                     /gene="ybeY"
                     /locus_tag="VNT12_09890"
     CDS             complement(1536..1946)
                     /gene="ybeY"
                     /locus_tag="VNT12_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWI46470.1"
                     /translation="MSVAVLDEVGHARLDTERASRLFAVALSTLGLDLERIGEVSVAL
                     VDPETIRGLNFEFRDRDEPTDVLSFEVDGPYGEMVGEVVICPNCADHGMSVDELVVHG
                     ALHLGGMEHGEDFGASEMARLQRAVMEELRGEES"
     gene            complement(1994..>3097)
                     /locus_tag="VNT12_09895"
     CDS             complement(1994..>3097)
                     /locus_tag="VNT12_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWI46471.1"
                     /translation="AGLSIIGTMLLGPRLMFLMVVITSVNIGILSGNNFLLTAALLLG
                     SGFAIYTTVRVDSRQRLLKAGLVIAAVMGVVTFAVGLIGGGGPQDALWLGVLGLGNGL
                     LSLMLAMVLMPFLEDAFNILTPMKLLELSDPRHPLLDKLLRNAPGTFSHSMQVGLLAE
                     RAAERVGADALLAQVGAYYHDIGKMEHPAYFVENQIAQINPHAALSPTLSAKVVKRHV
                     KDGLEIGEAWGLPREILDIIAQHHGSTRIEYFYRKALEDAPDEEEVRESDFRYSSGLP
                     RSKEAGIMMLADSIEATVKSLPKPTPKRIDDVVADTVRRKLQDGQFDECQLTMHDIHE
                     AGEAIREALIGFLGPRIAYPEDQGDARSGKAAS"
BASE COUNT          571 a          948 c         1001 g          577 t
ORIGIN      
        1 ggtcttgttg atggagacga tggccggggt acccgagccg gcgacgaggc gggcgacgta
       61 ccggtcgctg ctccccagtc cttccgaggc ctgcagcgca tcgaagagca agaggatgac
      121 atccgactcg gagaggctgt ccacgacttg ctcctgcatc ctggctcgca gggtgtcgcg
      181 ggccttctgg gagccggggg tatccacgaa gatcgcctgg tatccagggc cgttgcggac
      241 gccccggatc gggctgcgcg tggtctgagg ccgggatgag gtgatggaga ccttctggcc
      301 gacgagcctg tttaccaggg tgctcttgcc gacgttgggc ctgcctacta cggcgataaa
      361 gccgctgcgg agcccgttgt tttcgacgcc acgggctacc gctcccttca cagatcctcc
      421 ggagagaagg agttgggaag aatctggtcg aagggatagc gctctaaccc ccggccagtc
      481 tccacaacga cctccttgca accgaactcc ctcaagacct gacggcaggc cccgcaagga
      541 aagcatggcg aggtgttcgg gctgaagacg gcgacgagtt cgatctccct gtcttcaggg
      601 tctgcagttg ccatcattgt aactgcgttg cgctccgcgc agatcgagag cccgtaggag
      661 gcgttctcga cgttcgcccc tgagtgtatg gtgccgcctc ctgtcaggat ggccgcgcca
      721 acgtggatgt tgctgtacgg cgcgtatgcc cggtttgcgg cgacgcgtgc ggcctccatc
      781 acggcctcag ggtccaaggc tcctcctttc acaagagttg aaaggctgcc gtcgtaacga
      841 ggaccccgat gacggccccc attatcacct ggtagacgct gtgaatcccg ctctcgacgc
      901 ggctctggca gacgagcagg gccatgagga gtgagatcac ggagatgatc ccggcgtagg
      961 atcccgcgcc accctggacg gctatgaagc tcgcggcgac ccaccctgcg aaggcgacag
     1021 ccgcgtgccc tgaaggcatc ccgccggcga aggagttcga ggagtgggtc agcgccttgc
     1081 caaggagaac gatgagcacg atcacaccga gcgtgacgag cgtgaggtgg gccggctggc
     1141 gccttatgct ctgcagggtc gccagggaga gcggccccag atcgtctgcc aggacgagat
     1201 aaccgacgag cacggccccg acggtggaga ccaggacggc ccccgccgag acgtccttgg
     1261 cgagcttggc cagaggatga tattccctcg tggcaaggtc gacgacgaat tccatggctg
     1321 tgttgaacag ctccgtcacg aagaccagga gtatggtcag aacaaggacc gcgagttcca
     1381 gcttcctgac tccgaccagc aggctcgcta cgaggaccaa gacggccacg acgacgtgga
     1441 agcgcatgtt gcgctgggtg cggaccgcgt agatcatacc ccggtaggcg tgctcgaagc
     1501 tgcgggccac gccctgctgg cccttgcttg gctgctcaac tttcctcgcc acgcaactcc
     1561 tccattacgg cccgctggag cctcgccatc tcactggccc cgaagtcctc tccgtgttcc
     1621 ataccgccca agtgtagcgc gccgtgcacc acgagttcgt cgacactcat cccatgatca
     1681 gcacaattgg ggcagatcac gacctcccct accatctccc cataaggtcc gtctacctcg
     1741 aaactgagca cgtcggtagg ctcgtcccta tctctgaact cgaagttgag gccacggatc
     1801 gtctcggggt cgacgagggc cacggagacc tcgccgattc gctcaaggtc gagaccaagg
     1861 gtggagagag caacggcgaa gaggcggctg gctcgttccg tgtcgagtcg ggcatggcct
     1921 acctcatcga gaacggcgac ggacatggga ggggagttcc tcagatggcg actctgtccg
     1981 tggcgtccac agcctaactt gccgcctttc cgctgcgggc atcgccttgg tcttcaggat
     2041 acgcgatgcg cggcccgagg aaacctatga gcgcctcgcg gatggcctcc cctgcttcgt
     2101 ggatatcgtg catggtgagc tggcactcat cgaactggcc gtcctgaagc tttcttcgta
     2161 ctgtgtcggc gacgacgtcg tcgatacgct tcggggtcgg cttcggcagc gacttcacgg
     2221 tcgcctctat ggagtcggcg agcatcatga tgcccgcttc cttgcttcgg ggcaggccgc
     2281 tggagtagcg gaagtccgac tcgcgtacct cttcctcgtc gggggcatct tcgagggctt
     2341 tgcggtagaa gtattcgatc ctggtcgatc cgtggtgctg ggcgattata tctagaatct
     2401 cacggggcag tccccacgct tcacctatct cgaggccatc cttgacgtgg cgtttgacca
     2461 ccttggctga cagcgtcggg gagagggctg cgtgcgggtt gatctgggcg atctggttct
     2521 cgacgaagta agcgggatgt tccatctttc cgatgtcgtg gtagtaggcg ccgacctggg
     2581 ccaataacgc gtcggcgccg accctctccg cggccctctc agcgagcagg ccgacctgca
     2641 tggagtgaga gaaagtgccg ggtgcgttgc gcaggagctt gtcgagcagg ggatgtcggg
     2701 ggtcggagag ctcgaggagc ttcatcggcg tgaggatgtt gaaggcgtct tccaggaaag
     2761 gcatcagcac catcgccagc atcagcgaga gaaggccgtt cccgagtccg aggacgccaa
     2821 gccacagggc gtcctgaggg ccgccgcccc cgatcaaacc cacggcgaaa gtcactacgc
     2881 ccatgaccgc agctatgacc agtccggctt tcagcagccg ctggcgggag tccacgcgca
     2941 cggtggtgta gatggcgaaa ccagatccta gcagcagggc cgcggtcagg aggaagttat
     3001 tgccgctaag gatccctatg ttgacgctgg tgatcaccac catcaagaac atgagcctcg
     3061 gtccgaggag catggttccg atgatggaga gtcctgc
//
LOCUS       DATMJA010000416         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_410560, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000416 DATMJA010000000
VERSION     DATMJA010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410560"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VNT12_09900"
     CDS             complement(<1..432)
                     /locus_tag="VNT12_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HWI46472.1"
                     /translation="MNAYMHPYPFSAIVGQDDLKLALLVNAVSPEVGGVLVRGEKGTA
                     KSTAVRALASLLPSIRVISGCPYSCDPEAPNPECPAGPHPLGASAESRPVRLVELPVG
                     ASTDRLAGTLDIEKALSEGSKAFEPGLLAAAHRGILYVDEVN"
     gene            complement(429..>2230)
                     /locus_tag="VNT12_09905"
     CDS             complement(429..>2230)
                     /locus_tag="VNT12_09905"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HWI46473.1"
                     /translation="AELHRDLGVEEQPEEFSDFLTHVDGYLCEIKDLPIRGGLHVLGE
                     TPEGESLRHLLASILRLGAGEVPGLRRAACAAFGLDERVLAENGGTKVEAPPGLVERF
                     PGTVVTASDLLDRLEEVQQALLRGMEERGWDADSAGTVCEKTLGVADAGVESSLRIAA
                     EEVVPRLMRTPEEMGNLLSGLEGGYVPAGPSGSPTRGLINVLPTGRNFYSVDPKALPS
                     ALSWEVGQGLADDLLRRYLEEEGRYPEMVGIVVWGTAAMRTQGDDVSEILALLGVRPV
                     WNEESRRVVGLEVIPPGELGRPRIDVTVRISGFFRDAFPNLISLLDEAFTTVAELEEP
                     DDMNFVKKHAGEERSRGADERRSTTRIFGSKPGAYGAGLLPLMDARNWRTDEDLAEVY
                     AVWGGYAYGRGLDGVEARGVMESNLRRTEIAVKNVDNREHDLFDSDDYFQYHGGMIAA
                     VRALTGRDPKAYIGDSADPSRVKTRDLTEEARRVFRSRVANPKWITAMQRHGYKGAFE
                     LSATVDYLFGYDATAGVVEDWMYSDVTRKYVLDQEVRDFMQQSNPWALRAISERLLEA
                     AQRGLWSEPDAEDLEALKQVYLENEGMLEEGIS"
BASE COUNT          372 a          756 c          688 g          414 t
ORIGIN      
        1 attcacctcg tccacgtaca ggatgcccct gtgggccgcc gccagcaacc ccggctcgaa
       61 cgccttactc ccctccgaga gcgccttctc gatgtccaga gtcccggcga gacggtccgt
      121 gctcgccccc acaggtagct ccacaagccg caccggcctc gactcggcgg aggcaccgag
      181 cgggtgcggt cccgccgggc attccgggtt gggggcttcc ggatcgcagg agtagggaca
      241 gccagagatg acccgtatac tgggcaatag gctcgccagc gcccgcaccg ccgtggactt
      301 cgccgtccct ttctcgccgc gcacgagcac gccccctact tcgggggaga cggcgttcac
      361 gagcagggcc agtttcagat cgtcctggcc cacgatggct gagaatggat agggatgcat
      421 gtacgcgttc atgagatgcc ttcctccagc atcccttcgt tctccagata aacctgtttc
      481 aaagcttcaa ggtcttcggc gtccggttcg gaccagaggc cgcgttgcgc cgcttcgagc
      541 aggcgctccg agatggcgcg gagcgcccag gggttggact gctgcatgaa gtcccttact
      601 tcctggtcaa ggacgtactt cctggttacg tcgctgtaca tccagtcttc gacgacacca
      661 gctgtggcat cgtacccgaa gagatagtcc acggtggcgg agagctcgaa ggcgcccttg
      721 tagccgtggc gctgcatggc cgttatccac ttcgggttgg cgacgcggct ccggaagacg
      781 cggcgggcct cttcggttag gtccctcgtc ttcactctcg atgggtctgc ggagtctccg
      841 atgtacgctt tcgggtcgcg gcccgtaagg gcccggacgg cggcgatcat acctccgtgg
      901 tattgaaagt agtcgtccga gtcgaagagg tcgtgctcac ggttgtccac gttctttact
      961 gcgatctccg ttctgcgcag gttggactcc atgacccccc gcgcctcgac gccatctaac
     1021 cctctaccat aggcataccc tccccagaca gcatagacct ccgcaagatc ctcgtccgta
     1081 cgccagttgc gggcgtccat cagggggagc aggcctgcgc cgtaggcgcc tggcttggac
     1141 ccgaagatgc gcgtggtcga gcggcgttcg tcggcgcccc ggctcctctc ctcgccggcg
     1201 tgctttttga cgaagttcat gtcatcgggc tcctcgagtt ccgcgacggt ggtgaaagct
     1261 tcgtcaagga gggagatcag attcggaaag gcatccctga agaagccgct gatcctgacg
     1321 gtaacgtcga tccttggacg tccgagctct cctggaggga ttacctcgag cccgacgacc
     1381 cgccgcgact cctcgttcca gacgggtcgc acaccgagca gagccaggat ctccgagacg
     1441 tcgtcgccct gggttctcat ggctgccgtc ccccacacca cgatgccgac catctccgga
     1501 taacggcctt cttcttcgag atagcgccgc agcaggtcgt ccgcgagccc ctgtccgacc
     1561 tcccacgaga gcgcgctcgg cagggctttc gggtccacgg agtagaagtt gcgccctgtg
     1621 gggagcacgt tgatcaggcc gcgcgtcggg gaaccggaag gtcccgcggg cacgtatcct
     1681 ccctccagcc cggagagcag gttccccatc tcctcgggcg tacgcatcag gcgcggaact
     1741 acctcctcgg ccgcaatgcg caacgaactc tccacgccgg cgtcggcgac gcccagagtt
     1801 ttctcgcaca ccgtacctgc agagtcagcg tcccagccac gctcctccat cccgcggagc
     1861 agggcctgct gcacctcctc cagcctgtcg aggagatcgg aagccgttac cactgtaccg
     1921 gggaagcgct cgacgagccc gggcggagcc tcgaccttcg tgccgccgtt ctctgcaaga
     1981 actctctcgt cgagaccgaa ggcggcgcat gcggcgcggc gcaaaccagg tacttcaccg
     2041 gcgccgaggc gtaggatcga ggcgaggagg tggcgcaggg actcaccctc gggcgtctcg
     2101 ccgaggacgt gcaggccgcc ccttataggc aggtctttga tctcacagag gtatccgtcg
     2161 acgtgtgtga ggaaatccga gaactcctcg ggctgctcct cgacgccgag gtcgcggtgg
     2221 agctccgcat
//
LOCUS       DATMJA010000417         6535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_469906, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000417 DATMJA010000000
VERSION     DATMJA010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469906"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VNT12_09910"
     CDS             <1..1011
                     /locus_tag="VNT12_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWI46474.1"
                     /translation="EELPRETLVPVRTPSKCESPHGVCQTCYGRALSTYRPVDVGEAV
                     GIIAAQSIGEPGTQLTMRTFHTGGIAGEDITAGLPRVVELFEARHPKAEATLAEIDGI
                     VSLEEADSDRQIKVVITAPDNSEVREYKVERQLLRPEFVEGAEVRANTPITEGSIYPH
                     AILENDLRYGRGSTTTERYLVDEVQKVYRAQGVDIHDKHIEVIVRQMLRKVIVDDPGD
                     TTLLPEQVADRFTVLAENARVEEEEGRAATYHTVLMGITKASLATDSFLSAASFQETA
                     RVLTDAAIEGKVDGLAGLKENVIIGKLIPAATGLPRYRRLTVAVEGYRTQDVDELDIA
                     AS"
     gene            1353..1721
                     /gene="rpsL"
                     /locus_tag="VNT12_09915"
     CDS             1353..1721
                     /gene="rpsL"
                     /locus_tag="VNT12_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWI46475.1"
                     /translation="MPTTNQLVRNEREKQTKKAATPALQGSPQKRGVCTRVSTTTPRK
                     PNSALRKVARVRLVNGQEVTAYVPGEGHNLQEHSVVLVRGGQVKDLPGVRYKVVRGAL
                     DTLGVNNRKQGRSKYGTKKS"
     gene            1733..2203
                     /gene="rpsG"
                     /locus_tag="VNT12_09920"
     CDS             1733..2203
                     /gene="rpsG"
                     /locus_tag="VNT12_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWI46476.1"
                     /translation="MPRRAAPPKKKIAADPVYGSVLVQQLVNKIMLDGKKSTSEKIVY
                     DALSYIEERTGTSPVQVLNNALDNIRPRLEVKSRRVGGATYQVPVEVDARRANTLALR
                     WMVGFARARREKTMGRRLAGEILDAKDNIGSSVKRKEDTHRMAEANRAFAHYRW"
     gene            2216..4363
                     /gene="fusA"
                     /locus_tag="VNT12_09925"
     CDS             2216..4363
                     /gene="fusA"
                     /locus_tag="VNT12_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWI46477.1"
                     /translation="MAVQVSRKIPLKRVRNIGIMAHIDAGKTTTTERILLYTGLTHKL
                     GEVHDGNAVMDWMAQERERGITITSAATTVFWGGIEGSSKNGKGTREGVHSRIADQHR
                     INIIDTPGHVDFTVEVERSLRVLDGAIALFDSVAGVEPQSETVWRQADKYGVPRIAFV
                     NKMDRIGADFYKAVGTMVDRLGANAVPVQLPIGAESEFQGIVDLVEMKAIVYTDDLGA
                     TWEVTEIPEEMRSLADEYREKLLEAVADQDEELMVMYLEGEEIEPDQIRAAIRQATLD
                     IRMTPVFVGSAFKNKGVQPLLDGVVDYLPSPLDVPPVGGKTLDGEEITREADENGPLA
                     ALAFKVQADPHVGKLTYVRVYSGTLKAGSYVMNTTKGRRERVGRLLQLHANSREQRDE
                     VYAGELVAAIGLSDTGTGDTLVSADDEEQIVLEEMIFPEPVIRQAIEPKTKADQEKLS
                     VALQRLAEEDPTFTVRSDEETGQTIVAGMGELHLEIIVDRLLREFRVDANIGRPQVAY
                     RETIRKRVEGIEGRFVRQTGGRGQYGHAVINAEHNDETGYEFDNKIVGGVIPREYIPS
                     VDKGIKDALDSGVIAGYPVVDVRVELVDGSYHEVDSSEMAFQVAGSMAIKEALKRANP
                     VLLEPVMDVEVVVPEEFMGDVMGDLSARRGHIQGMDSRGGGQVIRAMVPLSEMFGYAT
                     TVRSKTQGRATFTMQFDHYAEVPKSISAEIAER"
     gene            4370..5572
                     /gene="tuf"
                     /locus_tag="VNT12_09930"
     CDS             4370..5572
                     /gene="tuf"
                     /locus_tag="VNT12_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWI46478.1"
                     /translation="MSRGRFERTKPHLNVGTIGHVDHGKTTLTAAITRVLAKEVPDDP
                     ANREVAFDQIDNAPEERQRGITIATSHQEYATANRHYAHVDCPGHADYVKNMITGAAQ
                     MDGAILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKADMVDDPELLELVEMEVRE
                     LLSEYEFPGDDIPVVIGSALRALEGDDSEYGEPSVIQLLEAVDEYIPEPERDIDRDFL
                     LAVEDVFTIQGRGTVATGRVESGKIGLNEEIEIVGIRPTRKTVVTGVEMFNKSMDSAQ
                     AGDNIGALLRGVKRDDIERGQVLAKPNTITPHTRFKAEVYVLSKEEGGRHTPFFSNYR
                     PQFYFRTTDVTGEIRLEEGVEMVMPGDNTVMNVELISPIAMDEGLNFAIREGGRTVGA
                     GVVTQIVE"
     gene            5631..5780
                     /gene="rpmG"
                     /locus_tag="VNT12_09935"
     CDS             5631..5780
                     /gene="rpmG"
                     /locus_tag="VNT12_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWI46479.1"
                     /translation="MRQLVTLACEECKRRNYHSRKNRRNTPDRIQLQKYCPWCRHHTA
                     HRETR"
     gene            5795..5867
                     /locus_tag="VNT12_09940"
     tRNA            5795..5867
                     /locus_tag="VNT12_09940"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5828..5830,aa:Trp,seq:cca)
     gene            5898..6146
                     /gene="secE"
                     /locus_tag="VNT12_09945"
     CDS             5898..6146
                     /gene="secE"
                     /locus_tag="VNT12_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWI46480.1"
                     /translation="MGKKRASAGSNKKQSQNKSGGFLAGPRRFARDVRGELRRVSWPD
                     RDQLRQSTAVVLIIVIVLALYIAAVDFVFQSLVRLVFL"
     gene            6177..>6535
                     /locus_tag="VNT12_09950"
     CDS             6177..>6535
                     /locus_tag="VNT12_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HWI46481.1"
                     /translation="MKKWYVVNTYSGHENKVRTTLERRIDSMGLDRNFGEIRIPTESV
                     IEIKDGKKVPTIQRQFPGYVLVNMDMNDTTWQLVRQTPGVTQIVGAGEKPLALSRAEV
                     ERLLHPGEVETREKVKTT"
BASE COUNT         1447 a         1898 c         2132 g         1058 t
ORIGIN      
        1 gaggagctgc cgcgtgagac gctcgttccc gtgcggacgc cctccaagtg cgagagcccg
       61 cacggcgtct gccagacctg ttacggcagg gcgctctcga cttacaggcc cgtcgacgtc
      121 ggcgaggccg tcggcatcat cgcggcccag tccatcggcg agccgggcac ccagcttacg
      181 atgcgcacct tccacaccgg cggcatcgca ggcgaggaca tcacggcagg cctccccagg
      241 gttgtcgagc tcttcgaggc caggcatccc aaggccgagg cgaccctcgc cgagatcgac
      301 ggtatcgtct ccctcgagga ggccgactcg gacaggcaga tcaaggtcgt catcaccgca
      361 cccgataact ccgaagtccg cgagtacaag gtcgagcgcc aactcctcag gcccgagttc
      421 gtcgagggcg ccgaggtcag ggcgaacact ccgatcacag aaggttccat ctacccgcac
      481 gcgatcctgg agaacgacct ccgctacggc cgcggaagca ccacgaccga gcgctacctg
      541 gtggatgagg tgcagaaggt ctaccgcgcg cagggcgtgg acatccacga caagcacatc
      601 gaggtcatcg tacgccagat gctgcgcaag gtgatcgtgg acgaccccgg cgacaccacg
      661 ttgcttcccg agcaggtcgc cgaccgcttt acggtgctcg ccgagaacgc ccgcgtcgag
      721 gaagaggaag gcagggcggc cacctaccac acggtcctga tgggcataac caaggcctcg
      781 ctcgccaccg acagcttcct ctcggcggcc tctttccagg agacggcccg cgtccttacc
      841 gacgcggcca tcgagggtaa ggtggatggc ctcgcaggcc tgaaggagaa cgtcattatt
      901 gggaagctca tacccgcagc cacaggactg ccgcgttaca ggcggctcac cgtggccgtc
      961 gagggctaca ggacccaaga cgtcgatgag ctcgacatag cggcgtctta gacccgtcag
     1021 cctgtaagcg atcagccctc agcctcttcg gctgggggct accgctattc gacagagcaa
     1081 cgcggtcgtg cgagttccac ggcgcgaccc tctaggaggg tcgaatccga ccggagaggc
     1141 cgtcggctgg aggcaaaact gaagtgccga caagctgaaa tgctgaccag agcggccagc
     1201 tttcgctggc cgcccacggc ttgacatagc ggtcttctga gctaaaataa tgcgtcgcgg
     1261 ttacgagtcg ttaccaatgg gtagcaaagg cttcgtgccg tggcacgtga ttctgtaagc
     1321 ccctttgcac ggatagagag agaggcgcgt ccatgccgac gaccaaccag cttgtgcgca
     1381 acgagcggga gaaacagacg aagaaggcgg cgacgccggc actgcagggg tctccccaga
     1441 agcgcggcgt gtgcactcgc gtctcgacca ccacgccgag gaagccgaac tctgcgctgc
     1501 gcaaggtggc cagggtacgt ctggtgaacg gccaggaggt cacggcctac gttccggggg
     1561 aggggcacaa cctgcaggag cactccgtcg tgctcgtgcg tggaggacag gtcaaggacc
     1621 tgcccggcgt gcgttacaag gtcgtgcgcg gggcgctcga tacactcggg gtcaacaacc
     1681 gtaagcaggg ccgttccaag tacgggacca agaaatcgta ggaggatagt agatgccgag
     1741 gcgagcagcg ccaccaaaga agaagatagc cgccgatccc gtctacggga gcgtgctcgt
     1801 gcagcagttg gtcaacaaga tcatgctcga cggtaagaag agcacctcgg agaaaatcgt
     1861 ctacgacgcc ctctcttaca tcgaggagcg cacgggcacg agcccggtcc aggtactgaa
     1921 caacgccctg gacaacatca ggccacgtct tgaggtcaag agccggcgcg tcggcggcgc
     1981 gacctaccag gtgcccgtcg aagtcgatgc ccgcagggcc aacacgctgg cgctgcgctg
     2041 gatggtgggc ttcgcccgcg ccaggcgcga gaagaccatg ggccgcaggc ttgcagggga
     2101 gatcctggac gccaaggaca atatcggttc gagcgtgaag cgtaaggaag acacgcatcg
     2161 gatggcagag gccaaccggg ccttcgccca ttacaggtgg tgacgaggaa ctaatatggc
     2221 tgtacaagta tccagaaaga tcccgctaaa gcgggtcagg aatatcggca tcatggccca
     2281 catcgatgcc ggcaaaacaa cgacgaccga gcgcatcctg ctctatacgg gcctgaccca
     2341 taagctgggc gaggtccacg acggtaacgc cgtcatggac tggatggccc aggagcgcga
     2401 acgcggcata accataacct cagccgcgac gaccgtgttc tggggcggca tcgaaggctc
     2461 gagcaagaac ggtaagggca cccgcgaagg cgtccatagc cgcatagccg atcaacatcg
     2521 tatcaatatt atagacacgc ccgggcacgt ggacttcact gtagaagtgg agcgcagcct
     2581 tcgggtgctg gacggtgcca tcgcgctgtt cgactccgtg gccggagtcg agccgcagtc
     2641 cgaaacggtc tggcggcagg cggacaagta cggggtaccg cgcatagcgt tcgtcaacaa
     2701 gatggaccgc atcggcgccg acttctacaa ggctgttggc acgatggtcg acaggcttgg
     2761 ggccaacgcc gttcccgtgc agttgccgat aggcgcagag agcgagttcc aggggatagt
     2821 cgatctcgtg gagatgaagg ccatcgtcta caccgatgac cttggggcca cgtgggaggt
     2881 caccgagata ccggaggaga tgcgttcgct cgctgacgag tacagggaga agctcctgga
     2941 ggcggtcgcc gaccaggacg aagagctcat ggtcatgtat ctggagggcg aggagatcga
     3001 acccgaccag atccgggccg ccatccgtca ggccacgctg gatataagga tgacgcccgt
     3061 tttcgtcggt tctgcgttca agaacaaggg cgtgcagcca ctcctcgacg gggtagtcga
     3121 ctacctgccg agcccgctcg atgtgccgcc cgtggggggc aagaccctcg acggcgagga
     3181 gataacccgc gaggcggacg agaacggccc gcttgcggcg ctggccttca aggtgcaggc
     3241 cgatccgcac gtcgggaagt tgacctacgt gcgggtgtac tcaggcacgc tcaaggccgg
     3301 ctcctacgtg atgaacacca ccaagggcag gcgcgagcgc gtcgggcgtc tcttgcagct
     3361 gcacgccaac agccgcgagc agcgagatga ggtctatgct ggcgagctcg ttgcggccat
     3421 cggcctctcc gatactggga ctggggatac gctcgtttcg gcggacgacg aggagcagat
     3481 cgtgctggag gagatgatct tccccgagcc cgttatccgg caggccatcg agcccaagac
     3541 gaaggccgac caggagaagc tctccgtggc gctccagcgg ctcgccgaag aggacccgac
     3601 gttcaccgtg cgcagcgacg aggagacagg gcagaccatc gtagccggca tgggcgagct
     3661 gcacctcgag atcatcgtcg acaggctcct gcgcgagttc agggtggacg cgaacatcgg
     3721 ccgtccgcag gtcgcttacc gggagaccat ccgcaagcgc gtggagggca tcgagggccg
     3781 gttcgtgcgg cagaccggtg gccggggcca gtacggccac gcggtcatca acgccgagca
     3841 caacgacgag actggctacg agttcgacaa caagatcgtc ggcggcgtca tcccgaggga
     3901 gtacatcccc agcgttgaca aaggcatcaa ggatgcgctg gattctggcg tcatcgcggg
     3961 ctaccccgtg gtcgatgtca gggtcgagct ggtcgacggt tcttaccacg aggtcgactc
     4021 ctccgagatg gcgttccagg tcgctggttc catggccatc aaagaggccc tcaagagggc
     4081 caacccggtc ctgctggagc ccgtgatgga cgtcgaggtc gtcgtgcccg aggagttcat
     4141 gggcgacgtg atgggcgacc tctcggcgag gcgaggccac atacagggta tggactcccg
     4201 cggcggcggg caggtcatcc gcgcgatggt gccgctctcc gagatgttcg gttacgcgac
     4261 gaccgtgcgc tccaagaccc agggccgcgc tacgttcacc atgcagttcg atcattacgc
     4321 cgaggtgccg aagagcatat cggccgagat agcagaaagg taggagagga tgtcaagagg
     4381 caggttcgag cgcaccaagc cgcacctcaa cgtcggcacc ataggtcacg tcgatcacgg
     4441 caagaccacc ctaactgcgg ccataaccag ggtccttgcc aaggaggtcc cagacgatcc
     4501 ggccaaccgg gaggtcgcct tcgaccagat agacaacgct cctgaggagc gtcagagggg
     4561 cattacaata gccacctccc accaggagta cgcaacagct aatcgccact acgcacacgt
     4621 cgactgccct gggcatgctg actacgtcaa gaacatgata accggtgctg cccagatgga
     4681 cggggctatc ctggtggtca gtgcagctga tggtcccatg ccccagacaa gagagcacat
     4741 cctcttggct aggcaggtag gggttccata catagtggtc tacctcaaca aggcagacat
     4801 ggtcgatgac cctgagctcc ttgagttggt tgagatggag gtaagggagc tcctctctga
     4861 gtacgagttc cccggagatg acatcccagt agtcatagga tctgccctca gagccctaga
     4921 aggggatgat tcggagtatg gtgagccttc ggtaatacag ctccttgagg ccgtagatga
     4981 gtacatccca gagccggagc gagacataga tagggacttc cttctggcgg tagaggatgt
     5041 gttcaccatc cagggacgag gaacggtagc aacaggacga gtagagtcgg gcaagatagg
     5101 actcaatgag gagatagaga tagtaggcat acgtcctacc cgcaagactg ttgtaacagg
     5161 ggtagagatg ttcaacaaga gcatggatag tgctcaagca ggggacaaca taggagctct
     5221 gctaagagga gtaaagcgcg atgacataga gcgaggtcag gtattggcca agccaaacac
     5281 cataaccccg cacacccgct tcaaggcaga ggtatatgta ctttctaagg aagagggagg
     5341 tcgtcacacg ccgttctttt ccaactaccg tccgcagttt tacttccgca caacggatgt
     5401 gacgggagag atccgcctgg aagagggggt agagatggtg atgccagggg acaacacggt
     5461 aatgaacgtg gagttgatct caccgatcgc gatggatgag gggttgaact ttgccatccg
     5521 agagggagga aggacagtag gagcaggcgt cgtaacacag atcgtggagt aacgcgccgt
     5581 tggtgcacag ggacggtgac ttcgccgtcc ctgtgattcg gaggtaagtt gtgcgacaat
     5641 tggtaacgct ggcttgcgag gagtgcaagc gccggaacta ccacagcagg aagaaccggc
     5701 ggaacacccc ggatcgcatc cagctacaga agtattgccc gtggtgccgc catcatacgg
     5761 cgcaccggga gacccggtaa ggatcggcag acagagggca gtagctcagt tggtagagca
     5821 gcggtctcca aaaccgcagg tcgggggttc gagtccctcc tgccctgctt cgagaggcaa
     5881 agccatgcga ggacacaatg ggtaagaagc gcgcatccgc ggggtcgaac aagaagcagt
     5941 cgcagaacaa gagtggcggc tttcttgctg gaccgaggag gtttgccagg gacgtgcggg
     6001 gtgagctcag gcgggtaagc tggcccgacc gtgaccagct caggcagagc acggccgtgg
     6061 tgctcatcat cgtcatcgtc ctggcgctct acattgccgc tgttgacttc gtgttccaga
     6121 gtctggtgcg cctggtcttc ttatagatct tctttaggca ttaggagcgt ttcgttgtga
     6181 agaagtggta cgttgtaaat acatactcgg gtcacgagaa caaggtgcgc acgaccctgg
     6241 agcgcaggat cgactccatg ggcctcgacc ggaacttcgg tgagatccgc atcccgaccg
     6301 agagcgtcat cgagatcaag gacggcaaga aggtccccac gatccagcgc cagttccccg
     6361 gatatgtcct cgtgaatatg gacatgaacg acacgacctg gcagctcgtt cgccagacgc
     6421 cgggggtcac ccagatcgtg ggcgccgggg agaagccgct cgcgctaagc cgcgccgagg
     6481 tggagaggct cctgcatcca ggcgaggtcg agacacgcga gaaggtcaag acgac
//
LOCUS       DATMJA010000418         3547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_588225, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000418 DATMJA010000000
VERSION     DATMJA010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588225"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..2842)
                     /locus_tag="VNT12_09955"
     CDS             complement(266..2842)
                     /locus_tag="VNT12_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWI46482.1"
                     /translation="MSRQEQAEVKPREPEEGFSAIFELASVGAVLGDADGRILRVNPK
                     MCEITGYSKEELLGKTPADLTHPDDVEQDLEKFRQMAAGETPMHGREKRYVRKDGQIR
                     WVSVNATPIRDEAGRLLRIASVVSDITERKRVEATLERQARQEALRAEVSAALAGGGT
                     LPDILRWCVEAMVTHLGAAFARVWILDEEEDVLELRASAGMYTHTDGFHGRVPVGSFK
                     IGLIAQERQPHLTNDVLRDPRVHDKEWARREGMVAFAGYPLIVEGRVVGVMALFARQE
                     LAGDTIEALASVADVIAQGIQRKRAEQELTESRERYRAVVEQAAEGIYILDTENGRIV
                     ESNPALGEMLGYNAEEMQEMEVHDLVAHPREEVDANFERVLAEGQLLVGEREYCRKDG
                     SLIDVEVSASMIHFGTKEMVCVLVRDVTQRKKTEEALREMREAERSRIARDLHDGALQ
                     DLSYALAETRDALSVPENPRLDRRLGRAVEALERMGPELRSAIYDLRLEEERNKPLVE
                     SLRDLVELNHRMNPDLNIRLKVDESFPARPLGERGAELLRIIQEALTNVRRHSEARNA
                     TVSMMVEGALLVAEVADDGRGFDPAQATQAGIGTNGMHERARALKGDLKIESELGKGT
                     KVRFETRLKRKREKPEEEIHVMLVEDHASFRQAAASVFEREAGFEVVGQVGSLTEARK
                     ILEQRAPDVAVIDLVLPDGYGWDLIQELRAANRRAQALVLTAELGRAEIARAVEHGAA
                     GILHKTAELEEVVEAVQRLSAGEALLPLEEVVELLRFAGARKDEEYEAQQAIARLTPR
                     EREILQALAEGLDSHGIAERLYISLRTERNHMTNILAKLGAHSRLQALVFAVRHGVVS
                     ID"
     gene            complement(3279..>3547)
                     /locus_tag="VNT12_09960"
     CDS             complement(3279..>3547)
                     /locus_tag="VNT12_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI46483.1"
                     /translation="TRGVVFFLGGGFLELSGSSRTGTSESVGLWLQVRDVDAVGQKLE
                     EAGVDIIELPTDKPWGLREMQIRDPDGLSIVIVEVPETHPLRRR"
BASE COUNT          567 a         1223 c         1010 g          747 t
ORIGIN      
        1 gaaccgcgat taacggtcgg gaggcgcgac ctctcgtctc aataggtcgg tgaagcgtca
       61 tatggcttcg cctgcccgac gaccccgttt tgtgcgccgg tgcttcgtta acacaggcat
      121 ggggcttcgg acatgccacc gagactcggt gttgaaaggt gggcttctac gttgtgggcg
      181 ccagactatc gatgcctaag ggtcggatgc aatccgatag ggtgttcacg ctgagaccgg
      241 ggctgcgggc ccggctcgcg cgtgctcagt ctatgctcac tacgccgtgg cgcacggcga
      301 acaccagcgc ctgcaacctc gagtgcgccc cgagctttgc gagaatgttg gtcatgtggt
      361 tgcgctccgt cctgaggctt atgtagagtc gctcggcgat cccgtggcta tctaggccct
      421 cagcgagcgc ctgcaagatc tccctttctc taggggtaag gcgtgctatg gcttgctgag
      481 cctcgtactc ttcgtccttg cgagctcccg cgaaacggag tagctccacg acctcctcca
      541 ggggcagcag cgcctcgcct gcgctgagcc gctgtacggc ctcgaccacc tcctccagct
      601 cggccgtctt gtgcaagatg cccgcggccc cgtgctcgac cgcccgggct atctcggcgc
      661 gaccgagttc ggcggtcaga accagcgcct gcgcgcgccg gttggcggcg cgcaactcct
      721 gtattaggtc ccaaccgtaa ccatccggca agaccaggtc gatcaccgcc acgtccggcg
      781 cccgctgctc gaggatcttc ctggcctcag tgagtgagcc gacctgtccc accacctcga
      841 atcccgcctc tcgctcgaac accgatgcgg cggcctgccg gaaggaggcg tggtcctcga
      901 cgagcatgac gtggatctct tcttctggct tttctcgctt cctcttcagg cgcgtctcga
      961 accgcacctt ggtgcccttg cccagctcgc tctcgatctt caggtctccc ttgagcgcgc
     1021 gggctctttc gtgcattcca ttagtcccta tcccggcttg agttgcctgg gccgggtcga
     1081 agcctcgtcc atcgtccgcg acttcggcta ccagcaaagc gccctcgacc atcatgctca
     1141 ccgttgcgtt gcgggcctca gagtgtcggc ggacgttggt cagcgcctcc tggattatgc
     1201 gcagcagctc cgcgcccctc tctccgaggg gcctggcggg gaagctttca tctaccttca
     1261 gccgtatgtt gagatccggg ttcatcctgt ggttcagctc cactagatcc ctaagtgact
     1321 cgaccagcgg cttgtttcgc tcttcttcca ggcgcaagtc atagatggca ctgcgcaact
     1381 ccggtcccat tcgctcgagc gcctcgaccg cccgccccaa tcgccgatcc agccttgggt
     1441 tctccggcac cgacagggca tcccgcgtct ctgccaaggc gtaactgagg tcctggaggg
     1501 caccatcgtg gaggtcacgg gcgatcctgc tacgctcggc ctccctcatc tcccggagcg
     1561 cctcctcggt tttcttgcgc tgggtgacgt ccctcaccaa aacacacacc atctccttgg
     1621 taccgaagtg gatcatgctc gcgctgacct cgacgtcgat gagcgagccg tccttcctgc
     1681 aatactccct ctccccgacg aggagctgtc cctctgcgag gactcgctca aagttggcgt
     1741 cgacctcctc acgggggtgg gcgacgaggt catgtacctc catctcctgc atctcctccg
     1801 cgttgtagcc cagcatctcc ccgagcgccg ggttactctc cacgatgcgt ccgttttcgg
     1861 tgtccaggat gtagatgccc tcggcggcct gctcgaccac cgcccggtaa cgctcccggc
     1921 tctccgtgag ttcctgctca gcccgctttc gctggatgcc ctgggcgatc acgtcggcta
     1981 cggaagcgag tgcctcgatt gtgtctcctg cgagctcctg gcgggcgaac agcgccatca
     2041 cccccaccac cctgccctcg acgatgaggg gataaccggc gaaggccacc atgccttccc
     2101 gcctcgccca ttccttgtcg tggacgcggg gatcgcgcag tacgtcgttg gttaggtgcg
     2161 gctgccgctc ctgggcgatg aggccgatct tgaagctgcc gacgggcacg cgtccatgga
     2221 acccatcggt gtgggtgtac atccccgcgc tggcccgcaa ctccagcacg tcctcttctt
     2281 catcgaggat ccagacgcgg gcaaacgcag cacctaggtg tgtgaccatc gcctccacgc
     2341 accaccgcag gatgtcgggt agggtgccac ctccggcgag ggcggcgctt acttccgccc
     2401 tcaacgcctc ttggcgtgcc tggcgctcga gtgtcgcttc aacccgcttt cgctcagtta
     2461 tgtctgaaac aaccgaggcg atgcgtagca gtcgcccggc ctcgtcgcga atgggcgtcg
     2521 cgttgacact cacccacctt atttgcccgt ccttgcggac gtagcgcttc tcgcgaccgt
     2581 gcatgggcgt ctccccggct gccatctgcc gaaatttctc aagatcttgc tcgacatcgt
     2641 cagggtgggt tagatctgcg ggcgtcttgc cgagaagttc ttctttggaa tagccggtga
     2701 tctcacacat cttggggttg acgcgtaaga tccggccgtc ggcgtcgcct aggaccgcgc
     2761 cgacactcgc cagttcaaat atggcggaga agccctcttc gggctcccgt ggcttcactt
     2821 cagcctgctc ttgcctgctc acatcatgct ctcttctggt caagtagcag agcgttaagg
     2881 gcaatgataa caaggccgag gactcggcaa ccacacgagc aggcgaccgc gccgttgcta
     2941 ctctagaagg actccacgaa cgccaaccgg tcagcgtccc tctcagtcag caggacgtgg
     3001 ccgtgcggtt ctaaggggtg ggtgtttctg gactcacgcg tcgatcctgg ccccttcgct
     3061 ccaggcgagc ccggaccccg agtgccgaag ctcagtcgaa tgcgcctagg cccggtccgc
     3121 gaccccttct gctcctgcca tactagcgca gtgtgcacag caattagtac accgggcctt
     3181 atagaggacc cggggttgaa cctcctgcct accccccggc tgtttctttc cgccgcacat
     3241 aagtgagggg gttgcggcgg aaagaaacgc aacccagctc accgacgtcg gagtgggtgg
     3301 gtctctggta cctcgacgat cacaatactc aacccgtccg ggtctcgtat ctgcatctcc
     3361 ctcaatcccc atggcttgtc agtcgggagc tcgataatat ccactccggc ctcttccagc
     3421 ttctgcccta cggcgtcgac gtctctcacc tgcaaccaca gacctacact ctcgcttgtc
     3481 ccagtgcgcg aggagccaga cagctcaagg aagcctcctc cgaggaagaa aaccacgccc
     3541 ctggtgg
//
LOCUS       DATMJA010000419         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_56063, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000419 DATMJA010000000
VERSION     DATMJA010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56063"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VNT12_09965"
     CDS             complement(<1..180)
                     /locus_tag="VNT12_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46484.1"
                     /translation="MKRLAIVFFVVLAAALFYRSFGSAETGTGSLTLPQPAPNVGDDA
                     PVFRVKSVDDRVFELS"
     gene            342..560
                     /locus_tag="VNT12_09970"
     CDS             342..560
                     /locus_tag="VNT12_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46485.1"
                     /translation="MDGLVEASSDVERNVDLVEDLPDSARVGTRLGEVVVKVDGKKIG
                     ETRLLAEKGYEKASLGQRVWYTVEGIFE"
     gene            580..1539
                     /locus_tag="VNT12_09975"
     CDS             580..1539
                     /locus_tag="VNT12_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HWI46486.1"
                     /translation="MILTVTLNAAVARTLVVPSLTLGHRHRAPESMTLAGGKGVNVAR
                     SLRTLDVPVLAAGFAGGRNGDAIRDGLSEAAIPFDLVEIEGYSRTSTAIIDPMAGTQT
                     EINEYGPEVTPAETREFSRRLEYLMEYATAVVFAGSIPPNLDESFLVGLVRRARERGI
                     YTVVDSPPSVLRAVLKANPSLVSPNQHEAESVVGFDFIEEGDFPLGLTRLVEHGAANA
                     CITSPEGLSYLSTENGTVSSRAPRVEAASTVGSGDAYLAGLLAGLLHRNLATAEAVRL
                     AAGCAAANAETLGAGLFEARRAEELAESVQVEVSSVSLQESRT"
     gene            1627..1938
                     /gene="trxA"
                     /locus_tag="VNT12_09980"
     CDS             1627..1938
                     /gene="trxA"
                     /locus_tag="VNT12_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWI46487.1"
                     /translation="MAVAEVTDATFESDVLRSEKPVIVDFWAPWCVPCRRVSPILEEM
                     SESREDVTFVKVNVDDNPSTAMNYSISSIPTIARFEEGQVTQQAIGALPKNQLSAQLG
                     L"
BASE COUNT          413 a          612 c          667 g          353 t
ORIGIN      
        1 cgagagctcg aagacgcggt catccacgct cttcaccctg aatacggggg catcgtcgcc
       61 aacgttaggg gctggttggg gtagcgtgag cgagcccgtg ccggtctcgg cgctgccgaa
      121 agaacggtag aacaacgcag cggccaacac gacgaagaag acgatggcca ggcgtttcac
      181 gaaccctccg aaccaagtct gaagaggcac acgacataag gattgtatcc gctaccatgg
      241 accttgtcgt agaaccagga tcatcgtagt ctctgtaagc ccgtgttcga aacgtcgagt
      301 accttgcagg cgggagaaga gtgtagatct tatggcgcag catggatggt ctcgtggagg
      361 cgagttccga cgtggagcgc aatgtcgacc tggtagagga tctgcctgac tcggccaggg
      421 tgggaaccag gctcggtgag gtcgtcgtaa aggtcgatgg gaagaagatc ggggagaccc
      481 ggcttctcgc agagaagggt tacgaaaagg cctcgctcgg gcagcgggta tggtataccg
      541 ttgaggggat tttcgaatag gagacaggta gggagaggca tgatactgac cgtcaccctc
      601 aacgctgccg ttgccaggac gctggtcgtt cccagcctga cgctcggcca caggcacagg
      661 gctcctgaga gcatgacgct ggccggcgga aagggcgtca acgtggccag gtccctcagg
      721 accctggacg tgcccgtact tgctgccggg ttcgccggcg ggcgcaacgg tgacgccatc
      781 cgcgacggcc tctctgaggc ggccatcccg ttcgacctcg tcgagataga gggatattcc
      841 cgcacctcga cggccatcat cgaccccatg gcggggactc agaccgagat caacgagtac
      901 ggccccgaag taacccccgc cgagaccagg gagttctcca ggcgcctgga gtacctgatg
      961 gagtacgcta cggctgtcgt cttcgcgggc agcatcccac cgaatctgga cgagagcttc
     1021 ctggttggcc tcgtgcggcg cgcgagggag cgtggaatct atacggtggt ggactcgccg
     1081 ccatccgtgc tcagggccgt actcaaggcg aacccctcgc tcgtaagccc gaaccagcac
     1141 gaggccgaga gcgtcgtcgg cttcgacttt atcgaggaag gagatttccc tctcggcctc
     1201 acaaggctcg tcgagcacgg ggccgcgaat gcttgcatca cctcccccga gggcctctcc
     1261 tacctcagca ccgagaacgg caccgtctcg tcgcgcgcgc ccagggtcga ggcagcctcg
     1321 accgtaggga gcggcgacgc ctatctcgcc gggctcctcg ccggtctact gcaccgtaac
     1381 ctcgcgacgg ccgaagccgt tcgtctcgcc gcagggtgcg cagccgccaa cgccgagacc
     1441 cttggcgcag gcctctttga ggcgcgcagg gccgaagagc tcgccgaatc cgtgcaggtc
     1501 gaggtctcct cagtgagcct ccaagagagc cggacctagg gtactttcgg ggctccgaga
     1561 agcccccgct ggcgtgctag aatgatcggc gaagttcact tccagagact acggaggttc
     1621 gagatcatgg cagtagccga agtaacagac gcgactttcg agagcgacgt tctgcggtcg
     1681 gagaaaccgg ttatagtgga tttctgggcg ccttggtgcg tgccgtgccg gcgggtctcc
     1741 ccgatcctcg aagagatgtc cgagtcgcgc gaggatgtga cgttcgtcaa ggtcaacgtg
     1801 gacgacaatc cttcgacagc gatgaactac agcatctcga gcattccgac catcgcccgt
     1861 ttcgaggagg ggcaggtcac ccagcaggcg ataggagccc tgccgaagaa ccagctatcg
     1921 gcacagctcg gtctgtaatt gcgaggagca acactcccgg ttacaatctc tggcgcataa
     1981 gctccgtaga gccgggtaag cccggccgtc gcgcggggcg tcgtagaaga gtcctaagtg
     2041 atgag
//
LOCUS       DATMJA010000420         5005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_885607, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000420 DATMJA010000000
VERSION     DATMJA010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885607"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /gene="agaS"
                     /locus_tag="VNT12_09985"
     CDS             <1..872
                     /gene="agaS"
                     /locus_tag="VNT12_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose-6-phosphate ketose isomerase"
                     /protein_id="HWI46488.1"
                     /translation="EYFADDRPTLFVSFARSGNSPESVAATGFADRFLSECHHLVLTC
                     DRSGRLYEKHARSTDSFVLLMPEATNDRGFAMTSSFTSMTLAAWLVLGTVADPGELVG
                     RLAAAAKRLLATRQEDIRRLASSGYERIVFLGSGPLKGLAHESSLKLLELTAGKIVTY
                     FESPLGFRHGPKSILDDRTLAVSYISGDPYTRKYDLDLLAELKRSMRAGSVIAIAATD
                     AGTDDPSFWLLEGLEGVEDAALALPFVVVAQLLGLCFSLALGYTPDNPFPDQEVNRVV
                     QGVSIHPLPKRSR"
     gene            869..1861
                     /locus_tag="VNT12_09990"
     CDS             869..1861
                     /locus_tag="VNT12_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWI46489.1"
                     /translation="MMYLGVDGGGTKTALCLVDEGGHVAAQTQAPSCYYFAEGIDLVG
                     RVLQEGIDEVCEQASVTPADIRYAFFGLPTYGEVSGDVPTLDAMPKEILGHSRYACDN
                     DMVCGWAGSLGGVDGINVVSGTGSITYGERIGHGVRVGGWGDLFGDEGSAYWISIKGL
                     NAFTRMSDGRLPVGPLHEALRTHLELAADLDLVDVVLNRWQGGRSEIAALSRIVADAA
                     ESGDECAAQILSEAADELANLVDTTRRILDFGPEEKVPVSYSGGIFSVRSIVDGFEAA
                     LEDLHDGYELRKPLYSPVIGAAIYAARLAGEPLDAETLPPTGAKRTNRPYSGGF"
     gene            2116..4968
                     /locus_tag="VNT12_09995"
     CDS             2116..4968
                     /locus_tag="VNT12_09995"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWI46490.1"
                     /translation="MSLFASLGRFTYHRRWPIVLFWAVVLVTAALFAPKLGGQLKGGG
                     FDGSGGDSERVQDLMIEKFGLSRTNLVIVFESDKLSARSEEYQRAEDEALEPLRHIEE
                     IHSVTTYADTKDQRFLSEDGNKTYALVGFNVSVAETQSLVDDVRRKLRSDELTTYVTG
                     PPAVYLDITNASSEDIRRAEKYAFPLALVILVIAFGTLVAAGIPILIGGVGVITALAA
                     LYFLAGVYDMSVYVLSIATMLGLGLGIDYALFAVSRFREELETYPIPEAVSRTVATAG
                     RSIFFSGLAVLIGLSGLLFFPFMFIRSIGVAGVIVVFVSVCAALTLLPAILGVLGHKV
                     NVLAVRRNRGAVGAGFWGRSAEAVMQHPVLVILIVGVILSALLYPVTHMKIGIPEASV
                     LPEKYESRAGDDILKQDFDYASLYPMQIVATVQEGPLSAEGLEDIKQLGESVRETDGV
                     RSVESIYTIGETAARQYAEEVSKARKEAEAEARGRIDALVEERYNQLEQEQIQKEVEE
                     QTSQLAADQGTLPPGAEEQIRSQVEQEVSRRLEESGARERIRSEVESEVQQRIDEQVP
                     NLPDGISANGEITAQGVANFMKLPEARDSEELQDAKDDLVAGDLTLLRSVTEASPYVG
                     AARETVGDVRSVEPPKGVSFLVGGLSAGQKDFISSVYAKAPYAAAFVLGVTYLVLLYT
                     FRSVILPLKAVAVNILSLTASFGAMVFIFQDGNFSKVLNFTPLGFVDATLPILMFCTV
                     FGVSMDYEVFLLSRIREAYENGDSNTASVAKGLVSTAGIITSAAAIIIVVTGAFTFSS
                     VIITKAVGLGLAVAVFVDATIIRVLLVPATMRVLGKWNWWPGGRQRSRTVGVSHRRRS
                     TRTDRFFDILNMPIVVLGVLLAVVSVNLFLYFGYYSKTSTPLPAEHTVPSTTIERTER
                     TGPKKGTRPEKTRPATTLQSTIPTDQRATASATSSP"
BASE COUNT          995 a         1472 c         1541 g          997 t
ORIGIN      
        1 aagaatattt tgccgatgac aggcccaccc tgtttgtctc gttcgcgcgc tcgggaaaca
       61 gcccggagag cgtggcggcg accgggttcg ccgaccgttt tctctcggag tgccaccatc
      121 tggtgctgac ttgcgacagg agcggtcgac tctatgagaa gcacgcgcgg tcgactgact
      181 cgttcgtcct tctaatgccg gaggctacga acgaccgagg tttcgccatg acgtcgagct
      241 tcacgtcgat gacccttgcg gcctggctgg tcttgggtac cgtagccgac cctggcgaac
      301 tggtcggacg tcttgcggca gccgcaaaac ggttactggc cacccgccag gaggacatcc
      361 gacggttggc ctcctcgggc tacgagcgga tcgtcttcct cggcagcggt cctctcaaag
      421 ggctcgccca tgaatcctcc ctcaaactcc tggagttgac cgcaggcaag atcgtgacgt
      481 acttcgagtc tccattgggt ttcaggcacg gaccaaagtc catcctcgac gatcggacgc
      541 tggccgtctc ctacatctcg ggggatccgt acacgcgtaa gtacgacctg gacctcctcg
      601 ccgagctgaa acgttcgatg cgagccggga gcgtcatcgc gatcgccgcg acggatgcgg
      661 gaacagatga tccaagcttc tggcttctgg aaggtctgga gggagtggag gatgcggcgc
      721 tcgcactgcc gttcgtcgtg gttgcccagc tcctgggcct ctgcttctcg ctcgccctcg
      781 gctacacacc ggacaatccg ttccccgacc aagaagtgaa tcgcgtggta caaggcgtat
      841 ccatccatcc tctaccgaag aggagtcgat gatgtatctc ggcgtcgatg gtggaggcac
      901 gaagactgct ctttgtcttg tagatgaagg tgggcatgtc gccgcgcaga cacaagcccc
      961 gtcctgctac tatttcgcag aaggcataga ccttgtgggc cgggtattgc aggagggaat
     1021 cgacgaagta tgcgaacagg cctcggttac accggcggac attcgctacg cgttctttgg
     1081 acttccgacc tacggtgagg tgagcggcga cgtacccact ctcgatgcga tgccgaaaga
     1141 gattctcgga cacagccgct atgcctgcga caacgacatg gtctgcgggt gggcgggttc
     1201 gctgggcggt gtcgacggca taaacgttgt gagcgggacc ggatctataa cctacggtga
     1261 gaggattggg cacggcgtgc gggtcggggg ttggggtgac ctcttcggcg acgagggctc
     1321 tgcgtactgg atctccatca aggggcttaa cgcgttcacg cgcatgagcg acggacgctt
     1381 gccggtcgga ccccttcatg aagccttgcg cactcacctg gagctggctg ctgacctgga
     1441 tctagtcgat gtcgttctca accgttggca gggcgggcgg agcgagatcg cagccctcag
     1501 ccggatagtc gccgatgcgg cggaaagtgg ggatgaatgc gcggcgcaga tcctatcgga
     1561 ggcggcagat gaactcgcaa acctggttga taccaccaga cgtatcctgg atttcggtcc
     1621 tgaggagaag gttccggtat cctactccgg cggtatattc agtgtacgct ccatcgtcga
     1681 cggcttcgaa gcggccctcg aggatcttca cgacgggtac gagctgcgga agcccttgta
     1741 ttcgcctgtt atcggtgcgg cgatctacgc ggccagactc gcgggcgaac cactggatgc
     1801 cgagacgctt cccccgacag gagcgaagcg gacaaaccga ccgtatagcg gagggttctg
     1861 acaggcatcc accgatggtc gatggatgcc tgcggacggg tccacagagc atgtcagggc
     1921 tgcaccagcg tcacctctac gcaatactgt aatcacagga ggctcggtgg gctttctgcc
     1981 tgttcgcggc gacggccaag actagttttg acagctggct cataatccct gctgaatcac
     2041 atcccttcct gatcacgtaa ggggcgtcgg ttactataga gacatgaaag aaccttgcgc
     2101 caagggtagg cttcattgag cctgttcgcg tccctgggcc ggtttaccta ccaccgccga
     2161 tggccgatcg tattgttctg ggccgtggtg ttggtgacgg cggctctttt cgcgccgaag
     2221 ctcggtggcc agctaaaggg tggggggttt gacggctcag gcggagattc cgagcgggtg
     2281 caagacttga tgatcgagaa gttcggtctc tcgcggacaa atctcgtaat agtctttgag
     2341 agcgataagc tttccgcaag aagcgaggag taccaaaggg ccgaagatga ggcgctcgaa
     2401 ccgctccgcc acatagagga gattcattcc gtaaccacct acgctgatac caaggatcag
     2461 cgtttcttgt cagaggacgg taataagacc tacgcccttg tcgggttcaa cgtctctgtg
     2521 gccgaaacgc agagcctggt agacgacgtc cgcaggaagc tgcgctccga cgagctgacg
     2581 acctacgtaa cgggacctcc tgccgtctac ctggacataa cgaacgccag ctccgaggat
     2641 atcaggcggg ccgagaagta tgcgttcccg ctggcgctgg tcatcctggt gatcgccttc
     2701 gggacactgg tggcggccgg gatcccgatc ctgatcggtg gtgttggcgt cataacggca
     2761 ctggcggcgt tgtacttcct ggccggcgtc tacgacatgt ccgtctacgt gctgagcatc
     2821 gccaccatgc tggggctcgg gctcggcatc gactacgcgc tcttcgccgt gagccgcttc
     2881 cgggaagagc tagagaccta cccgattcca gaggcggtgt cgagaactgt ggcaacggcg
     2941 ggaaggtcca tcttcttctc ggggctcgcg gtcttgatcg ggctttcagg attgctcttt
     3001 ttcccgttta tgtttatccg ctctataggg gtcgccggcg tcattgtggt gttcgtgtct
     3061 gtctgcgcgg cgttgacact gctgcctgcg atcttgggcg tcctcggaca caaggtgaac
     3121 gtgctcgcgg tgcgcaggaa ccggggtgca gtgggggctg gtttctgggg caggagcgca
     3181 gaggccgtaa tgcagcatcc ggtgctcgtt atcctgatcg tgggagtgat actctccgcc
     3241 ctgctctacc cggtaaccca catgaagatc ggcatccccg aggcgagcgt cctgcccgag
     3301 aagtacgaat cccgcgccgg ggacgacatc ctcaagcagg acttcgacta cgccagcctt
     3361 tatccgatgc agatcgtcgc caccgtgcaa gagggtccac ttagcgccga gggcctggaa
     3421 gacatcaagc aactcggcga gagcgtacga gaaaccgatg gtgtacggag cgtcgagagc
     3481 atctacacta tcggcgaaac ggccgcccgc cagtacgcgg aagaggtatc caaagcccgc
     3541 aaagaggcag aggccgaagc gcgaggaaga atcgacgctc tggtagaaga gcggtacaac
     3601 cagctcgagc aggagcagat ccaaaaagag gtagaggagc agacctcaca actggcagcc
     3661 gatcagggaa ccttaccgcc tggagccgaa gagcagatcc ggtcccaggt agagcaggag
     3721 gtcagcagac gtctggagga gtccggagcc agagaacgga tacggtccga ggtagagtcg
     3781 gaagttcagc agcgaataga tgagcaggtt ccgaacctgc ctgatggtat ctccgccaac
     3841 ggagagatca ctgcccaagg tgtcgccaac ttcatgaaac tacccgaagc ccgtgactcg
     3901 gaggagctac aggacgcgaa agacgatctc gtggcgggag atctcacgct gctgcggagc
     3961 gtaaccgagg cgagccccta cgtcggggca gcccgcgaga ccgtcggtga tgtgcgctcg
     4021 gtcgagccac ccaagggcgt aagcttcctc gtcggcggcc tctctgcagg acagaaggac
     4081 tttatctcca gcgtctacgc caaggccccc tacgcggcgg cgttcgttct tggggtaacc
     4141 tacctggtac tcctctacac cttccgctcc gtcattctcc cgttgaaggc ggtcgcggtg
     4201 aacatcctga gcctcacggc gagcttcggc gcgatggtct tcatcttcca ggatggtaac
     4261 ttctcgaagg tgctcaactt tacgccgctg ggcttcgtgg acgccaccct cccgatcctg
     4321 atgttctgca cggtcttcgg ggtctccatg gactatgagg tatttctgct ctcccgcatc
     4381 agggaggcct acgaaaacgg cgactccaac accgccagcg tcgcaaaggg gctggtgtcc
     4441 accgcaggaa tcattacgtc agccgctgcc attatcatcg tcgtgaccgg tgccttcacc
     4501 ttcagcagcg tcatcattac caaagcggtt ggccttgggc tcgccgtggc cgttttcgta
     4561 gacgcaacga tcatccgcgt tttactcgtt ccggccacca tgcgcgtcct cggcaaatgg
     4621 aactggtggc ccggcggccg ccagaggagt cgcacagtag gagtctccca tagaaggagg
     4681 tcgacgcgaa cggacaggtt tttcgacatc ctcaacatgc cgatcgtcgt acttggcgtg
     4741 cttttggcag tggtgtccgt aaacctcttc ctttatttcg gctactactc caagacctcg
     4801 accccgctgc ctgccgagca tacggttcct tcgacgacta ttgaaagaac agagcgtaca
     4861 gggcccaaaa aggggacgcg gcccgagaag acaagaccag caaccaccct ccagagcacg
     4921 atccccaccg atcagagggc cactgcgagc gctacctcct ctccgtagcc cagccgaaca
     4981 ccccgatcgc caagcgaagc ttctc
//
LOCUS       DATMJA010000421         3783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_707461, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000421 DATMJA010000000
VERSION     DATMJA010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707461"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(345..893)
                     /locus_tag="VNT12_10000"
     CDS             complement(345..893)
                     /locus_tag="VNT12_10000"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HWI46491.1"
                     /translation="MDGVEGPLLALTLVLMFVGLLGSVLPGLPGVTLIFLSALVYALL
                     TDFRTVGAAILVILFVFAAIAFVADFVATSYGARRFGASNWGTAGGAIGGIAGALIGL
                     LFLGIGSLFGLILGTIAGVFLGEYLKRTRQGQQQPGSTGPDWRRASHAAGGVLVGYLA
                     SAVVQGLLGLASVIVFVLALLY"
     gene            complement(897..1751)
                     /gene="larE"
                     /locus_tag="VNT12_10005"
     CDS             complement(897..1751)
                     /gene="larE"
                     /locus_tag="VNT12_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWI46492.1"
                     /translation="MNPVTTDILHKRLTDLEQILAPYESALVAFSGGVDSSLALAVAA
                     RALPKNRVLAVTSNNETYLPSELDLAREFAESLGVEHLVVNTRELDNPDYASNPTNRC
                     YFCKSTLYSDLAELAEQKGYACIVDGANKDDEGDYRPGRKAARELGVVSVLSEAGMTK
                     TEVRELAKHLDLPTWDKPALACLSSRFPYGQEITPEKLSQVARAEEFMRREGFSQVRV
                     RHHGEIARLEVGPEELDRAFAMREEISAELLDAGFLYVTLDLAGYRSGSLNAALKKSK
                     KALPLISS"
     gene            complement(1765..2337)
                     /locus_tag="VNT12_10010"
     CDS             complement(1765..2337)
                     /locus_tag="VNT12_10010"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWI46493.1"
                     /translation="MDGPENVYEVPQGLPEPEDDGACDHLPGMRMPSVSLTSTAGDAV
                     DLSTLTRTTVVYCYPLTGRPDQDLPPGWDDIPGARGCTPQSCAFRDHHAELKELGARV
                     FGLSTQDTAYQREAAGRLHLPFLLLSDEKLEFARALGLPTFEAEGMTLIKRLTLVIEE
                     GEIKHVFYPVFPPGRNAEEVVRWLSERRTD"
     gene            complement(2346..3587)
                     /locus_tag="VNT12_10015"
     CDS             complement(2346..3587)
                     /locus_tag="VNT12_10015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWI46494.1"
                     /translation="MADRRPEAYITGIGAVTPIGVGRKKLWSSLLANESGAGPITLFD
                     PEGFEVRIAAECTDFDPESFMDRKVAARTDRFGQMGLASAKLALEDAGAWDMLNNDPE
                     RVGLILGSGIGGVTSMEATQATIDSKGPSRVNPFAVTKIMPNSAAAHVGIQLGVQGPS
                     ITGALACTCGTDMMGLGYDLIRRGDADIVLCGASEAGLAPVIVAGFIAMRAMSRRNED
                     PEGACRPFDVDHSGFLIGEASAVVVLESAESVGRRGSDPYAKITGVGRTTDAYNLTDP
                     DPKGFGIMRAMQLAVADAGLRGEQIGYINPHGTGTVAGDGPESQAMAKINPDVKVSAT
                     KSTLGHSLGASGAVETAICALAIKERKIPPMRNLTQVADGCADLDYVVDEPREAPDLE
                     AALCANLGVGGHNAAVVLERA"
BASE COUNT          659 a         1193 c         1210 g          721 t
ORIGIN      
        1 gccgtctccc aggattcaga tctctcctcc tacgtcagga tgctcgaaga acgcttcgac
       61 tcccaggcag atcagggtcc gcgcaacctg ccatcaggcg acgaactcgc ccaggaactc
      121 gaaggtttcc tccgcgagca ccgtcgcgaa gaagaatagc acttcagctc gttcagccct
      181 tcgggcttcg cttataagca cggcgccctg caggcgcctt gtcagcacgc ttcgggccta
      241 taggccctcg ctctcagcat gtcagccagg acttgcgtgt gcgaaagcgg aagtgctgac
      301 tagctgacaa gccgtacggc gaagccgggc ggcgtgctga aatgctaata caacagcgcc
      361 aatacgaaga ctatgacgct ggcgagacca agcagtccct gtacgacggc ggaggccagg
      421 tagccgacga ggacgccgcc tgccgcgtgc gaggcgcggc gccagtcggg tcccgtggag
      481 ccaggctgtt gttggccctg acgcgtgcgt ttgaggtatt caccgagaaa gacgcctgcg
      541 atggtaccca ggatgaggcc gaacagggag ccgatgccga ggaagaggag tccaatcagg
      601 gcgccagcga tgccgccgat ggcaccaccc gcggtacccc agttgctcgc tccgaagcgg
      661 cgggcgccgt atgaggtggc gacgaagtcg gcgacgaagg ctatcgcggc gaatacgaag
      721 aggataacca ggattgcggc gccgacggtg cgaaagtctg tgagcaaggc gtagacgagg
      781 gctgacaaga agatgagcgt tacgccgggc agaccaggca ggacgcttcc gagcaggccc
      841 acgaacatca acacgagggt gagggcgagc aacggaccct ctacgccgtc catagtctac
      901 gacgagatga ggggaagagc cttcttgctc ttctttaggg cggcgttcag gcttcctgac
      961 ctgtagcctg cgaggtcgag agtgacgtag aggaagcctg cgtcgaggag ttcggcggag
     1021 atctcctccc tcatggcgaa ggccctatcg agctcctcag ggccgacttc gaggcgggct
     1081 atctccccgt ggtgcctgac acggacctgc gaaaagccct ctctacgcat aaactcttcg
     1141 gcgcgcgcga cctgggagag cttctcgggt gtgatctcct gcccgtaagg gaaacggctc
     1201 gacaggcacg cgagagccgg cttgtcccag gttggcagat ccagatgctt cgccaactcc
     1261 cgcacctcgg tcttggtcat ccctgcttcc gagagcacgg atacaacccc gagctcccta
     1321 gcggccttcc tgccaggacg gtagtcgccc tcgtcatctt tgttcgcccc gtcaacgata
     1381 catgcgtaac ctttctgctc agcgagctcc gccaggtcgg agtagagcgt gctcttgcag
     1441 aagtagcagc ggttcgtagg gttgctcgcg tagtccgggt tgtcgagctc gcgggtattt
     1501 acgacgaggt gctccacccc gagggattcg gcgaactccc tcgccagatc caactctgat
     1561 ggaagatagg tctcgttgtt cgacgtgacg gcgaggaccc tgtttttcgg tagcgccctc
     1621 gctgcgacgg cgagggcaag cgaggaatcg accccgccgg agaaagcgac gagcgcgctc
     1681 tcgtagggag cgagtatctg ttcgagatcc gttagcctct tgtgcaaaat gtcggtcgtg
     1741 acggggttca tctccacgtc ctctctagtc agttctacgt tcggagagcc agcgcacgac
     1801 ctcttcggcg ttcctgcctg gcggaaacac cgggtagaag acgtgcttga tctcgccttc
     1861 ctctataacg agggtcaggc gctttatgag ggtcattccc tcggcctcga acgtcggcag
     1921 ccctagcgct ctcgcgaact ctagcttctc atcactcagc aacaggaacg gaaggtgaag
     1981 ccgccctgcg gcctcgcgct ggtaggcggt gtcctgcgta ctgagcccga agacccttgc
     2041 gcccagctcc tttagctccg cgtgatggtc gcggaaggcg cacgactggg gggtgcagcc
     2101 acgagcgccg gggatatcgt cccacccagg tggcaggtcc tggtcaggcc tccccgtgag
     2161 cgggtagcag taaacgacgg tcgtccttgt cagggtcgag aggtccacag cgtctcccgc
     2221 cgttgacgtc agggatacgg aaggcatcct catgcctggc aggtggtcgc aggcgccatc
     2281 gtcttccggc tcgggtaggc cctgtggtac ctcgtatacg ttctctggtc cgtccatgcg
     2341 gctcctcagg ctcgttccaa gacgaccgct gcgttgtgcc cgccgacgcc gaggttggcg
     2401 cagagggcgg cttccaggtc gggcgcctcg cgcggctcgt ccacgacgta gtcgaggtcg
     2461 gcgcaaccgt cagctacttg cgtaaggttt ctcatcgggg ggatctttct ctccttgatg
     2521 gcgagggcgc agatcgcggt ctccacggcg ccgctcgccc ccagagagtg gcccagggtg
     2581 gatttcgtgg ccgagacctt gacgtcaggg ttgatcttgg ccatggcctg cgactcgggg
     2641 ccgtcaccgg ccacagtacc cgtgccgtgc gggtttatgt agccgatctg ctcccctctc
     2701 agtccggcgt cggcgacggc gagctgcatg gcccgcatga tgccgaagcc tttcggatcc
     2761 gggtcggtga gattgtacgc atcggtggtg cggccgacgc cggtgatctt cgcgtaaggg
     2821 tcggacccgc ggcgccctac ggactcggcg ctctccaaca ccaccacggc cgacgcctcg
     2881 ccgatcagga accctgagtg gtcgacatcg aagggcctgc acgccccttc ggggtcctcg
     2941 ttacggcggc tcatcgcccg catggctatg aatccggcga caatgacggg cgccaggccg
     3001 gcctccgagg cgccgcagag cacgatgtcg gcgtcaccgc gccggatcag gtcgtagccc
     3061 agccccatca tgtccgtgcc gcaggtgcag gcgagcgcgc cggtgatcga gggtccctgg
     3121 acgcctagct gtatgcctac gtgggccgcg gcggagttgg gcatgatctt ggtcacagcg
     3181 aaggggttta cgcgggaggg tcccttgcta tctatggtcg cctgggtcgc ttccatgctg
     3241 gtaacacccc cgataccgct gccgaggatg aggccgaccc gctcagggtc gttgttgagc
     3301 atgtcccagg cgcccgcatc ttcgagggcc agcttggccg aggcgaggcc catctgcccg
     3361 aaccggtcgg tcctggccgc cactttgcgg tccataaagc tctcggggtc gaagtccgtg
     3421 cattcggcgg cgatgcgcac ctcgaagccc tcgggatcga agagcgttat ggggccggcc
     3481 ccgctctcgt tggccaggag cgagctccag agcttcttgc gccctactcc tatgggggtg
     3541 accgcaccga tccccgttat gtaagcctcc gggcgcctgt ctgccatctc gccccctagc
     3601 tgaatccaag atccatcgaa ctacgaatct tagcattaca cgcccccggc gactccggcg
     3661 taactcgccc tgtgctataa ccctgcagga tgagtcggtg gagtgagcat tgatcttcta
     3721 catcgcgatg gtcctctttg ttgcgaactt cgcgctcggc gtactcgtgc tgtttcgtat
     3781 cgt
//
LOCUS       DATMJA010000422         6977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_471164, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000422 DATMJA010000000
VERSION     DATMJA010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471164"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VNT12_10020"
     CDS             <1..222
                     /locus_tag="VNT12_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWI46495.1"
                     /translation="PLYVANEGKLLAVVAPEAADCALEALRNTEGGEEAQTIGEVREE
                     PAHMVVMHTQFGGTRMIDMLVGDPLPRIC"
     gene            270..749
                     /locus_tag="VNT12_10025"
     CDS             270..749
                     /locus_tag="VNT12_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46496.1"
                     /translation="MNREPLTELSAILWRHLFTRFPEWQEYAEIVESEASDELNSLYV
                     RVYSPADEKSYLSIIERADCIEVSFSDGDPPGGAESQIICEEESESSCVEAALELIQE
                     IIDEKVVIGRERSTWLTGHKPLSPQFIVSREIVGKRRRLVSVRSWKGHFDWEQAGLI"
     gene            801..1709
                     /locus_tag="VNT12_10030"
     CDS             801..1709
                     /locus_tag="VNT12_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI46497.1"
                     /translation="MAGVCEGPARIVVMHTPFCGTRMIDMFVGDPVAEGRAMERKDVW
                     AAGDLYEPYVGRWSRLVAREFLDWLSRPTNEDWLDVGCGTGALTRVIIDTTQPRTVTG
                     IDPSAEFVAYSQEQIAGPGVSFKVGDARWLPVDASSVDVAVAGLILNFVPDPSQAVAE
                     MTRAVRAKGVVAAYVWDYAGKMEMMRYFWDAAGSLDPAALDFDEGRRFPICAPAPLLE
                     LFESAGLRSIDVRPIDVLMRFRDFDDYWRPFLGGQGPAPGYAMSLTEDRRNVLRDRIH
                     GHLPVLKDGSIELTARAWAVAGRVSS"
     gene            1816..2079
                     /locus_tag="VNT12_10035"
     CDS             1816..2079
                     /locus_tag="VNT12_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWI46498.1"
                     /translation="MAERIEKKEFIHRLAGRMQTDEDTAAQWLDGVLEEMYQTFRSGY
                     GLTLPGFGGFYLDRRRESWAFKFNPGQKLRALFGWSSSYRGPL"
     gene            2209..2676
                     /locus_tag="VNT12_10040"
     CDS             2209..2676
                     /locus_tag="VNT12_10040"
                     /inference="COORDINATES: protein motif:HMM:NF040080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HWI46499.1"
                     /translation="MREKYEAITEITDEFCQKHLNEDYADLSRKMAAALCEEEPSPLE
                     SGRERSWAAGIVYALGRVNFLSDDSREPHMTMSELCEKIGVSQGNASSKSREIWSRLG
                     LIQMDPDWCLPSMLEENPLVWIIEVDGLPVDVRMMPREVREQAYRLGLIPYMP"
     gene            2759..2995
                     /locus_tag="VNT12_10045"
     CDS             2759..2995
                     /locus_tag="VNT12_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46500.1"
                     /translation="MKTAISIPEKLFESAEQFARGRGMSRSELYATALRQYLEEHRGE
                     MITGRLDEIYGQESDGLDRDIARLQARSLPEDDW"
     gene            2995..3333
                     /locus_tag="VNT12_10050"
     CDS             2995..3333
                     /locus_tag="VNT12_10050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWI46501.1"
                     /translation="MRRGEVWWAELPEPVASEPGHRRPVLIVQSDDFNRSRIRTVIAV
                     VLTTNLRLAEAPGNVPVSKEDTALSRDSVANVSQIITLDKTFLTERVTRISDRIMMSV
                     EEGLRTVLAL"
     gene            3402..4583
                     /locus_tag="VNT12_10055"
     CDS             3402..4583
                     /locus_tag="VNT12_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWI46502.1"
                     /translation="MSQIDTDALTVQSREELLRRNDIFEGFFEVEAPRLAQICYEMSR
                     RFLAGGRLLAFGNGSAATDAQHVSVEFVHPVIVGKRALPALDLGPDFERRLPVLLQPK
                     DMVMGFAFPKADEPVERAIQAARERGALTFSLTGEVGEYSFAPPDRDPFVVQEIFEVL
                     YHVLWETVHVYFEHREQGHDVGNSSFLYPFLGKGQQPLEEVVREVQGSMLQKMQEVNR
                     LRTAAAESEARTISEIAAAVSGRLERGGKLIAFGNGGSATDANDLVSDCVDPPPGFGK
                     IPAVSLSAEPANVTAIANDIGTEAIFARQLIAHARPEDVAVGISTSGGSVNILAALAE
                     ARRRGLLTVGIVGYDGGRIVAERLANHAVVVRSDYIPRIQEVQASIYHVLRGVVERRM
                     A"
     gene            4621..4803
                     /locus_tag="VNT12_10060"
     CDS             4621..4803
                     /locus_tag="VNT12_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46503.1"
                     /translation="MLLIVGGLMILMGGVWLLQGISILPGSFMTGQMFWAIVGAVFLV
                     VGGLLALAGFRLNRHR"
     gene            4911..6236
                     /gene="hemL"
                     /locus_tag="VNT12_10065"
     CDS             4911..6236
                     /gene="hemL"
                     /locus_tag="VNT12_10065"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWI46504.1"
                     /translation="MQGSGESADARTMLAMDRSARLMERAVGLMPGGVNSPVRAFRLV
                     GGDPLFMERGEGSRIFDVDGNSYIDYVMSYGPLVLGHSYPEVIEAIETAARKGTTFGA
                     PTELEVELADLVRGAVPSVEMVRMTNSGTEATMSAIRLARGYTERERILKFDGNYHGH
                     GDALLVSAGSGVATLGLPNSPGVTRGAARDTAVLPYNDLGAVRDLFAREGEQFAAIIL
                     EPVAGNMGCVPPKKGYLEELREITNDYGALLIFDEVMTGFRLALGGAQERFGVTPDLT
                     CLGKIIGGGLPVGAYGGRREIMDQVAPLGPVYQAGTLSGNPLAMAAGLVTLRRAGQPG
                     FYGRLEELGSRWREGMGEAASAGSRPFTINQVGSMVSIFFTGGPVTDFGSAAASDTGE
                     FKDFFWHMLSRGVYLAPSQYEAGFISTAHSEVDLQRTFEAAREWFDRRA"
     gene            6237..6656
                     /locus_tag="VNT12_10070"
     CDS             6237..6656
                     /locus_tag="VNT12_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46505.1"
                     /translation="MEERASRTPELSLEDTFLKAVAGLPEEDLRAGVVAEALGYLQQG
                     FDAHYAGDGATDDDVLVGDNAYALAVETIARLDEPRFVAVASRMIRDGAGRIAAGEAV
                     SLQTWTPHLAGLLDIISEEGEERSEARITEAAEELRG"
     gene            6649..>6977
                     /locus_tag="VNT12_10075"
     CDS             6649..>6977
                     /locus_tag="VNT12_10075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI46506.1"
                     /translation="MGNLGKGTRNRVPSGEQRALLVREMFDRIAGRYDLMNTVMTAGL
                     HRLWNRKVVRTTGLRTGGRALDLACGTGTLTRDLAEKVGPDGYVLGVDFSREMLLAAR
                     SAPNVEYR"
BASE COUNT         1367 a         1964 c         2298 g         1348 t
ORIGIN      
        1 cccctctacg tcgcgaacga aggcaagctc ctcgccgtgg tcgcccccga agcagccgac
       61 tgcgccctcg aagccttgcg taacacagaa ggcggcgaag aagcccagac catcggcgag
      121 gtccgcgagg agccggcgca catggtcgtc atgcacaccc agttcggcgg cacccgcatg
      181 atagacatgc tggtaggaga ccctttaccg aggatatgtt gaggagattg tggccctgat
      241 ctcgcgatcg ggacgagtgg agatcatagg tgaacagaga acctctaact gagctctctg
      301 cgatcttgtg gcggcacctt ttcacccgat tccccgaatg gcaggagtat gccgagatcg
      361 tagagtccga agcgagtgat gaactcaaca gcctgtatgt acgagtttat tccccggcgg
      421 atgaaaagag ctatctatcg attatagaaa gggccgactg catcgaggtg agtttcagcg
      481 atggagatcc acccggcggg gccgaaagcc agataatttg cgaggaagag tcggagagtt
      541 cgtgcgtcga ggcggccctt gaattgatcc aagagatcat agacgagaag gtggtcatag
      601 ggcgcgagcg ttctacctgg ctcactgggc acaagccctt gtctcctcaa ttcattgtct
      661 cgagggaaat agtaggaaag agaagaaggc ttgtcagcgt tcgttcctgg aagggccact
      721 ttgattggga gcaggcaggt ctaatatgat ggcacaatcc aacacccaag tatgctgact
      781 gcaaggcgct gcggagcgcc atggccgggg tgtgcgaggg gccggcacgg atagtcgtga
      841 tgcacacccc gttctgtggc acccgcatga tagatatgtt tgtcggggac cccgtggcgg
      901 agggacgggc gatggagcgt aaggacgtgt gggcggctgg tgatctttac gagccctatg
      961 taggtcgatg gagccgactc gtggcgagag agttcttgga ttggttgtcc agaccgacaa
     1021 acgaggactg gttggatgtc ggttgcggaa ccggcgctct tacgcgagtg attatcgaca
     1081 ccacgcagcc tcgcaccgta acaggcattg atccttccgc tgagttcgtc gcctactctc
     1141 aggaacagat cgcaggtccg ggagtgtcat tcaaagtcgg agatgcccga tggctacccg
     1201 tcgatgcgtc gagcgttgac gtcgccgtgg ctggtttgat tctcaacttc gtccccgacc
     1261 cctcgcaggc tgtcgccgag atgacccgcg ccgtccgtgc caagggtgtc gtagctgctt
     1321 acgtctggga ctatgcgggg aagatggaaa tgatgcggta cttctgggat gccgctgggt
     1381 cgctcgatcc cgccgctctt gacttcgatg aaggccgacg attcccgatt tgcgcgccag
     1441 cgccattgct cgaattgttc gagagcgcag gcctgcggag tatcgatgtt cgtcctatcg
     1501 acgtcttgat gcggtttcgt gattttgacg actactggag gccttttctc ggtggtcaag
     1561 ggccggctcc cggttacgcc atgtcactta ctgaggatcg gcgcaacgtg ctccgtgatc
     1621 gaatacatgg acatcttcca gttctaaaag atgggtccat agagttgacg gcacgtgcgt
     1681 gggctgtggc aggacgtgtc tcctcatgac tgaacagacc ccaagtatgc ggctcaactc
     1741 gccgcagcgc gcgttgtcgc ctggattaga gagataggct gaagtcctgt tggagtcgag
     1801 agtgaaagga aacctgtggc cgaacgtatc gagaagaagg agtttatcca ccgcctcgcc
     1861 gggaggatgc agactgatga agacacggct gcgcagtggc tggatggtgt gctcgaggag
     1921 atgtaccaga ctttccgctc cggctacggg ctcacgctgc ccggctttgg aggtttctac
     1981 ctggaccgaa gacgagagag ctgggccttc aagttcaacc ccggtcagaa gctgcgcgcc
     2041 ctcttcggct ggtcctcctc ttaccgcggc cctctgtagt acgagacccg cacctgacgt
     2101 gtacactcat ttcggcgagg acgcgcagga tacgaaaaac cctggtggtt ccgaagtttg
     2161 gagacgatcg agatggatgc gggagatgga tctagggtcc cccgaataat gagggagaaa
     2221 tatgaagcca ttaccgaaat cacggacgag ttttgccaga agcatctgaa tgaggactac
     2281 gcggacctgt ctcgaaagat ggctgccgcc ttgtgcgaag aggagccatc accgctcgaa
     2341 agcgggcgtg aaagatcatg ggccgccggc atcgtctacg cgctcggccg ggtgaacttc
     2401 ctctccgacg atagccggga gccgcacatg accatgagcg agctttgcga gaagatcggc
     2461 gtgagccagg gcaacgcttc gagcaagtcc agagagatct ggagccggct gggcctcata
     2521 cagatggacc cggattggtg tctgccgagc atgctggagg agaacccgct ggtgtggatc
     2581 atcgaggtag atggtctgcc cgtagatgta cgcatgatgc ccagggaagt acgggagcaa
     2641 gcgtatcggc tgggcctgat cccttatatg ccttagaacg ctctgatgcc agtgagatgg
     2701 tacgatcgtc cgttttgcgc ctctcgcctt ctggtataac tcgggttata ctccgggcat
     2761 gaagaccgcg atctctattc ccgagaagct tttcgagtct gccgagcagt tcgctcgggg
     2821 acgtggtatg tctcgtagcg agctgtatgc gacggcgttg cgtcagtacc tggaggagca
     2881 tcggggcgag atgattacgg ggcggctcga tgagatctat ggccaggagt cggatggctt
     2941 ggaccgagat atcgctcgcc tgcaggcccg gtcgttgccg gaagatgact ggtagtgcgg
     3001 cgcggagaag tctggtgggc ggaactgccc gagcctgtcg cctcggaacc tggacaccgg
     3061 cgacctgtgc tcatcgtcca gtcagacgac ttcaaccgca gccgtatccg tactgtgatc
     3121 gcggtggtgt tgacgacgaa cctgcggctg gcggaggctc ctggtaatgt tccggtttct
     3181 aaggaggata cggcgttatc cagagactcc gtggcaaacg tgtcgcagat aatcacgctc
     3241 gacaagacgt ttcttactga gcgggtgacc cggatcagcg accgtataat gatgtccgta
     3301 gaggaaggcc tgcggacggt gctcgccctg tagaactcgg cgcgcttccc gtgtagggaa
     3361 tcatttatcg gaggccgact tgacggctgg aggtgcaggc gatgagccag atagatacgg
     3421 acgccctcac tgttcagagc cgcgaggaac tgctcaggcg caatgatatc ttcgaaggat
     3481 ttttcgaggt ggaggcgccg aggctggcac agatatgcta cgagatgtcc cgccgtttcc
     3541 tggcaggagg acggctcctg gcgttcggga acggttctgc ggctaccgac gcgcagcacg
     3601 tctcggtcga gttcgtgcac cctgtgatcg ttggcaagag ggcgctgcca gcgctcgacc
     3661 tcggacctga cttcgagcgt cgcctgcccg tcttgctaca gcccaaggac atggtgatgg
     3721 gcttcgcgtt cccgaaggcg gacgagccgg tcgagcgggc gatccaggca gcgcgggagc
     3781 ggggagcgct aacgttcagc ctgacggggg aggtggggga atattctttt gcgccgcctg
     3841 acagggaccc gtttgtcgtt caggagatct tcgaagtcct ctaccatgtt ctctgggaga
     3901 cggtccacgt atacttcgag caccgcgaac agggccacga cgtcggcaac tcctccttcc
     3961 tttacccctt cctcggcaaa ggacagcagc cactcgaaga ggtcgtgcgc gaggtgcagg
     4021 gctcgatgct tcagaagatg caggaggtga accgcctgcg cacagcggcg gccgaatcgg
     4081 aggccaggac catctccgag attgcggccg cggtgtccgg acgactggag cggggtggaa
     4141 agctgatcgc cttcggtaac ggcggctcgg ccacggacgc gaacgacctt gtctcagact
     4201 gcgtagaccc accaccgggt ttcggtaaga ttccggcggt ctccctgtcc gctgagccgg
     4261 ccaacgtaac ggccatagcc aacgacatag gcacagaggc gatcttcgcc cgccagctca
     4321 tcgcgcacgc taggccagaa gacgtcgctg ttgggatctc cactagcgga ggttccgtaa
     4381 atatactcgc cgcgctcgcc gaagcccgaa gacgaggttt actgaccgtc ggcatcgtgg
     4441 gctacgatgg aggtaggatc gttgctgagc ggctggcgaa tcacgcggtc gtagtccgct
     4501 cggactacat cccgcgcatc caggaggtcc aggcttccat ctaccacgta cttcgcgggg
     4561 tcgttgaacg gcgcatggcc tgagccccgg atctggacct ggaggtatcg tatacgctac
     4621 gtgctgctga tcgtaggagg gttgatgatc cttatgggcg gcgtctggct gttgcagggc
     4681 atcagcatac tgcccggcag cttcatgacc ggacagatgt tctgggccat agtgggcgcg
     4741 gtctttttgg tcgttggtgg attgctcgcc ctcgccgggt ttcgcctgaa caggcatcgc
     4801 tgagagtagg tcagcccgcc gatgccccat ttggggtaag ctctgtcacg gccagcaccg
     4861 gtccagggtt ataaacttca aacagatgta gcgaaggagg gctggcgcgt ttgcaaggta
     4921 gcggagagag cgcagacgcc aggacaatgc tcgccatgga ccgctcggcc aggctgatgg
     4981 aacgggccgt cgggctcatg cctggcggcg taaacagccc cgtgcgggca ttccgcctgg
     5041 tcggtggaga cccgttgttt atggagcgcg gcgagggttc gcgcatcttc gatgtggacg
     5101 gcaactccta tatcgactac gtgatgagct acggtcccct cgtcctcggg cattcttacc
     5161 ctgaggtcat cgaggctata gagactgcgg cgaggaaagg tacgactttc ggcgccccga
     5221 cggagctcga ggtcgagctc gcggacctgg tgcgcggggc agtcccttcc gtcgagatgg
     5281 tcaggatgac caactcagga acggaggcga cgatgagcgc catccggctc gcccgcggat
     5341 atacggaaag agagaggatt ctcaagttcg acggcaacta ccacggtcac ggcgacgccc
     5401 tcctcgtctc ggccggctcg ggtgttgcaa ccctcggcct ccctaatagc cccggcgtca
     5461 caaggggtgc cgcaagggac acagccgtcc tgccttacaa cgacctcggg gcggtccggg
     5521 atctcttcgc gcgcgaagga gagcagttcg ccgccatcat cctggaaccc gtcgcaggaa
     5581 acatggggtg cgttccgccc aagaagggtt atctcgaaga actgagggag atcacaaacg
     5641 actacggcgc gctacttatc ttcgatgaag tcatgaccgg tttcaggctc gcccttgggg
     5701 gggcgcagga gcgtttcggc gtcacgccag acctgacgtg cctaggtaag atcatcggcg
     5761 gcgggctccc tgtcggggcg tacgggggga ggcgcgagat catggatcag gtcgcaccct
     5821 tgggccccgt ctaccaggca ggcacactct cgggtaaccc gctcgcgatg gccgcaggcc
     5881 tcgtgaccct gcgacgcgca gggcaaccag gcttctacgg ccggctggaa gaactcgggt
     5941 cgcgctggcg ggaaggcatg ggcgaagccg cctctgccgg aagtcgaccc ttcaccataa
     6001 accaggtcgg ctcgatggtg agcatcttct tcaccggcgg tcccgtaacg gacttcggat
     6061 cagcggctgc ctcggacacc ggggaattca aggacttctt ctggcacatg ctctcccggg
     6121 gggtctacct ggctcccagc cagtatgagg ccggctttat ctccacagcc cactctgagg
     6181 tggaccttca gaggaccttc gaggctgcgc gcgagtggtt cgataggagg gcctaggtgg
     6241 aagagagggc cagccgcacg cctgagctct cgctggagga cacgtttctc aaggccgtcg
     6301 cgggccttcc cgaagaggac ctgcgcgccg gggtcgtggc cgaggcactc gggtacctgc
     6361 aacaaggctt cgacgcccac tacgcggggg atggggcgac cgacgacgat gtcctggtcg
     6421 gggacaacgc gtacgcgctg gcggtcgaga cgatagcccg tctggacgag ccgcggttcg
     6481 tcgctgtggc gagtcgtatg atccgcgacg gcgcaggacg catcgccgcc ggagaagccg
     6541 tctctctaca gacgtggact ccccacctcg caggcctcct cgacatcatc tcggaggagg
     6601 gagaggagag atccgaggcg aggatcaccg aggccgctga ggagctgcgt gggtaacctc
     6661 ggcaagggaa cccgcaacag ggtgccttcg ggggagcagc gtgcactgct ggtgcgcgag
     6721 atgttcgacc ggatcgcggg ccgctacgac ctcatgaaca ccgtaatgac cgcgggactc
     6781 caccgcctct ggaacaggaa ggtggtcagg acaacggggc tgaggaccgg cggtagggcg
     6841 ctcgacctcg cctgcggtac ggggacactc acgcgcgacc tcgcagagaa ggtcgggcct
     6901 gatggctacg ttttgggcgt cgacttctcc agggagatgc tgctagctgc acgctcagca
     6961 cccaacgtcg agtaccg
//
LOCUS       DATMJA010000423         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_353366, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000423 DATMJA010000000
VERSION     DATMJA010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353366"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..290
                     /locus_tag="VNT12_10080"
     CDS             105..290
                     /locus_tag="VNT12_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46507.1"
                     /translation="MGHAHSASTHTDGLLLGVGDRGIVVEGGELVRFYPWTSVGSIEK
                     THEEGGENPGVQAGRAW"
     gene            complement(344..1084)
                     /locus_tag="VNT12_10085"
     CDS             complement(344..1084)
                     /locus_tag="VNT12_10085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI46508.1"
                     /translation="MSTGHPKLYAELAGWWQLISPTEDYADEAAFFGRLFRAENAGTV
                     LELGCGGGNVARFLKEDFVLTLTDISEAMLAESKKQNPELEHVEGDMRTLRLERTFDG
                     ILIHDAIMYMSSEEDLRAALLTAYEHCKPGGVAVFVPDWVAETFQPGTTHEGVDRGDR
                     GVRYLEWTWDADPNDTKVNYEFILALKEGDELRAVVDRQVVGIFPRATWLRLLEDVGF
                     VPEVVEDPSSGGERVEVFVGHKPLPPTG"
     gene            complement(1123..2079)
                     /locus_tag="VNT12_10090"
     CDS             complement(1123..2079)
                     /locus_tag="VNT12_10090"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWI46509.1"
                     /translation="MARVAVLRGGHSMERDVSFLTGRRVQHALERLGHDVHPLDIEDT
                     TTGTLMEIAPDAAFICLHGPGGEDGTVQALLETLNIPYTGSGPLSSIRCMDKDYAKRA
                     LRAAGIQTPPFRTFLRRAMNEMGASAALDVAAEELGYPLVVKPAREGSSFGLSRVEGA
                     EGLFDAVYEALGYDAKILLERWVEGTEISIPIIEPAGEEPRALGLVEIRPREGAYNFE
                     AKYTPGATDFAIPAEIPSEAASRVEETALTVYKILGCSGFARVDTIFAGGTPWVLDVK
                     TIPGFTETSTFPLAAETAGTTFEMLVGTILETALRTEATAKL"
BASE COUNT          363 a          664 c          704 g          434 t
ORIGIN      
        1 cgctatgctg tagtcgttgc tggcggccac gcagggaggc ggcggtgtcg gtgacgatgt
       61 cggagaagga gataccctag acctgggttg gacagcgcgt cagggtgggc cacgctcaca
      121 gcgcgtccac ccacacagac gggttgttgc ttggggttgg cgaccgaggc attgttgtcg
      181 agggcggcga gcttgttcgc ttctacccct ggacgtccgt tgggagtatc gagaagaccc
      241 acgaggaggg tggcgagaat cctggcgtac aggcgggccg ggcctggtag caaccgcctc
      301 gcagcgatag acggactgcg tgaggaagct gcgggcacgt gttctacccg gttgggggca
      361 gaggtttgtg gcccacgaag acctcgacgc gttcaccgcc gctcgaaggg tcttcgacga
      421 cttctggcac gaacccgacg tcttcgagca atcgtagcca ggtcgcgcgt gggaagattc
      481 caacgacctg acggtccacg acggcgcgca gttcgtcgcc ctctttcaaa gccaggatga
      541 actcgtagtt gactttggtg tcgttcggat cggcgtccca ggtccattcc aggtacctga
      601 ctccccgatc tcctcggtct accccttcgt gcgtggtgcc tggctggaag gtctcggcca
      661 cccagtcggg tacgaacacg gcgactccac ctggcttgca gtgctcgtag gcagtgagca
      721 gggctgcgcg tagatcctct tcgctgctca tgtacatgat ggcgtcgtga ataaggatgc
      781 cgtcaaacgt cctttccagg cgcaatgtgc gcatgtcgcc ctcgacgtgt tcgagctcgg
      841 ggttttgctt cttgctctcg gcgagcatcg cctccgagat atcggtcaac gtcaggacga
      901 aatcctcttt gaggaaccgg gcgacattgc cgccaccgca gcccagctcg agcacggtcc
      961 cggcgttctc ggcccggaag agccttccga agaaagcggc ttcgtctgcg tagtcttcgg
     1021 tcggcgagat caactgccac cagcctgcca gctcggcgta caatttcggg tgcccggtac
     1081 tcactctacc tacttcgccg ccgttacatt aggacgaaaa ttctacagct tggccgttgc
     1141 ttccgtacgt agcgccgttt cgaggatggt cccaacgagc atctcgaagg tggtgcctgc
     1201 ggtctcggcc gcaaggggga aggtcgatgt ctctgtgaag ccggggatgg tcttcacgtc
     1261 gagaacccag ggggtgcctc cggcgaagat ggtatccacc ctggcgaaac cggagcagcc
     1321 aaggatcttg tagacggtaa gcgccgtctc ctctacgcgg gaggcggcct cggacggtat
     1381 ctctgccggg atggcgaagt ccgtcgcccc gggagtgtat ttagcctcga agttgtaggc
     1441 gccttcgcgg ggacggatct cgaccagacc tagggctctt ggctcttcgc ccgccggttc
     1501 gatgatgggg atggagatct ccgttccctc cacccagcgc tccagcagga tcttggcgtc
     1561 gtagcctagc gcctcgtaga cggcgtcgaa cagtccttca gctccctcga cccgggagag
     1621 cccgaagctc gacccttccc tggctggctt gacgacgagc gggtacccca gctcctcggc
     1681 ggcgacatcc agcgccgcag aagcccccat ctcgttcatc gcccttctca ggaaggtgcg
     1741 gaaggggggg gtctgtatgc cggcggcgcg gagcgcacgt ttggcgtagt ccttgtccat
     1801 gcagcggatg gaagagagcg gcccgctccc tgtataaggg atgttcaggg tctccagcag
     1861 cgcctgcacc gtgccgtcct cgccgccggg gccatgaagg cagatgaagg cggcgtcggg
     1921 tgcgatctcc atcagcgtcc ccgtggtcgt gtcctctatg tcgagcgggt gaacgtcgtg
     1981 gccgaggcgt tcgagggcgt gttggacgcg ccggcccgta aggaaggaga cgtcacgctc
     2041 catcgagtgt ccaccgcgca aaacggccac gcgcgccact gtattctaac cttccgtgcc
     2101 tgaactgaag cccactgtgc cgtgtatcgc gccttctttt cggtgcgagc cacgttccag
     2161 gtcgc
//
LOCUS       DATMJA010000424         6122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_293594, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000424 DATMJA010000000
VERSION     DATMJA010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293594"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VNT12_10095"
     CDS             complement(<1..511)
                     /locus_tag="VNT12_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HWI46510.1"
                     /translation="MPEKSRVAGLARRPDMAGTARRLLRLILVVVLLVGVSQGTTLAA
                     PTSWDYVALGDSLTTGFGAFKGYVPRYDAYIETDTGVAVTRTNLGRNGWTSSQLLSAL
                     RTDPTFRRATREAEIVTWNIGGNDLRAARNSYKKGTCGGADNQDCLRAGVAKLKTNWN
                     AITAEVLELR"
     gene            965..1444
                     /locus_tag="VNT12_10100"
     CDS             965..1444
                     /locus_tag="VNT12_10100"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWI46511.1"
                     /translation="MDNGEERTHRKQDLASVARHIIDSNAYMTLATADENGRPWASPV
                     WYAAEGYEHFYWVSSPEARHSRNVSARPEVGIVIFDSRAPVGTGQGVYVSAIAEELAG
                     TELDRGIGVYSRRSQTHGAGGWTREDVTPPARHRLYRATASEHSVLDDKDRRTLVRV"
     gene            complement(1525..1698)
                     /locus_tag="VNT12_10105"
     CDS             complement(1525..1698)
                     /locus_tag="VNT12_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46512.1"
                     /translation="MTEDVKTIMYPVTDLANAKTLYGELLGVEPYMDEAYYVGFNVEG
                     QDIGLDPHGHSKG"
     gene            1944..2831
                     /locus_tag="VNT12_10110"
     CDS             1944..2831
                     /locus_tag="VNT12_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI46513.1"
                     /translation="MEEQDWLAQRFETHRTRLRAVAYRMLGSLSEAEDAVQEAWFRLS
                     RSETSGIENLGGWLTTVVGRVCLDMLRSRTSRREEHLEEPQAARVSEPILGRDAGADP
                     EHEALLADSVGLALLVVLEMLSPAERLAFVLHDMFGVPYEEIAPIVDRSPNAAKMLAS
                     RARRRVRGKATVPDADLTLQREVVDAFLAASRGGDFEALLNVLDPDVVLRADSAAVQA
                     GAANEVRGAPAVADTFSGRARFARPALVNGAAGAVWAPGGRPRVVFGFKVTGGKIVEI
                     NLIADPARLRQLDLAVLDG"
     gene            complement(2872..3588)
                     /locus_tag="VNT12_10115"
     CDS             complement(2872..3588)
                     /locus_tag="VNT12_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWI46514.1"
                     /translation="MDSIEIVSSGSKSGVGVLDKAVAILAFLSGDGSASLSEVVEGTS
                     LSRPTAHRLLSALEMHHLVSRRDGRYVLGLRLLGWGNRAAGVGLVERARPVLEGLRDE
                     SGESTQLYVREGEQRVCVAAVERVTGLRDTVPVGAVMPLSRGSAGKVLLAWAQGGVDG
                     RPDAAQLRGIRVRGWAESVAEREAGVASVSAPVFGEDGRLRAAVSVSGPISRLGVSPG
                     QRLSQLVVGAARDIERTADP"
     gene            3633..4496
                     /locus_tag="VNT12_10120"
     CDS             3633..4496
                     /locus_tag="VNT12_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWI46515.1"
                     /translation="MILAREWDAGEYEKLSAPQTRWGVEVLERLVVRGDEAAIDAGCG
                     TGRVTELLLAKLPEGSVLAIDGSRAMVEAARRRFAGEPRVRVELQDLLSLDVVECVDL
                     IFSTATFHLIWDHDHLFENLARAIKPGGRLVAQCGGEGNIFRATRATKETMEEERFLD
                     YFVGWQDDKYYADAQSTARRLKAAGFEDVETWLHDEVAAFDSVDELARFLGTVVLGGH
                     LEMLPEEERSPFAARVAEKVAAVDGSPTLDYVRLNIMARRSALSLEGEGTNITSPATE
                     PPGVAETEEEA"
     gene            4493..5896
                     /gene="leuC"
                     /locus_tag="VNT12_10125"
     CDS             4493..5896
                     /gene="leuC"
                     /locus_tag="VNT12_10125"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWI46516.1"
                     /translation="MSGPRTLVEKVWEKHVVRSAEGEPDLIYVDLHLVHEVTSPQAFE
                     GLRLAGRGVRRPELTLATMDHNVPTSEFGLPIEDKVSAKQMEALRNNCEEFGIKLNEW
                     GSIGQGIVHIIGPETGLTQPGLVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     TQSRPKTMAVTVEGELPADVTAKDLMLGILNRIGTGGGVGHVIEYRGEAVRQLSMEGR
                     MTVCNMSIEGGARAGLVAPDETTFEYLEGREYAPKGEDWETAVEEWKKLPTDEGAEFD
                     KEVVIRAEDLVPYVSWGTTPAQTVPLDGEVPEPQNEGHERALKYMALQPGTPIRDIEV
                     DTVFLGSCTNARIEDLRAAAGVLEGHKVKEGIRAMVVPGSMRVKKQAEEEGLAEIFTE
                     AGFDWRNAGCSMCLGMNPDILQPGERCASTSNRNFEGRQGKGGRTHLVSPVVAAATAV
                     MGRFASPSELGVPLEVG"
     gene            5896..>6122
                     /locus_tag="VNT12_10130"
     CDS             5896..>6122
                     /locus_tag="VNT12_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWI46517.1"
                     /translation="MEPVERISGTALPLGYSDVDTDQIVPSDALKRIERTGFGQFLFS
                     GWREDEDFIMHREEHKDAVILIAGENFGSGS"
BASE COUNT         1129 a         1895 c         1997 g         1101 t
ORIGIN      
        1 aacgcaactc gagaacctcc gccgtgatgg cgttccagtt ggtcttgagt ttcgccacgc
       61 cagcccggag gcagtcctgg ttgtcggccc caccgcaggt ccctttcttg taagagttgc
      121 gggctgccct gaggtcgttc ccgccgatat tccaggtgac gatctccgcc tcacgggtgg
      181 cgcgccggaa cgtcggatcg gtgcggaggg cagagagcag ttgggaactg gtccatccgt
      241 tccggccgag gtttgtgcgt gtgaccgcta ctccagtgtc ggtctcaatg taggcatcgt
      301 agcggggcac atagcccttg aaggcaccga agcctgtggt cagagagtcg ccgagggcca
      361 catagtccca cgaagtgggt gcggcgagag tagttccctg cgacacaccg acgaggagga
      421 ccacgacaag gatgagcctc agcaagcgcc gtgcggtgcc tgccatgtct ggtcgccggg
      481 cgaggcccgc gactctgctc ttctccggca agatcccctt ctatgtcagc ctatgttcgc
      541 tggaactcta cactatcgag gagaatctat ggatccccat atgaatcatt tcttggagac
      601 ctctgcgaca tctggttccc gatccgacta ccaaaacgcc cattgcgcgg tgtcacgttg
      661 cctgattttt cgagttctct agctccggta cctggggaga gcgtgccgca cactgggcag
      721 agtgctcaat gtcacccgct ccgataagga gcagcttcta cgccaacact gtcgacaccc
      781 tcgatctcgt agacgtattc gagggtgaca cgctcaggat ctggttcgag gagctctctc
      841 gcgtactaca ggagcacctt cgacagggac ggcgatatcg accaggatcg ggtacagttg
      901 gcatcccgac tgtgtgtggc gggctgcggg cggggtaagc cgggccaggt agggaaggaa
      961 gagagtggac aacggagagg agaggacgca tcgcaaacag gacctcgcgt ccgtcgcgag
     1021 gcacatcata gactccaacg cctacatgac gctcgccaca gccgacgaga acgggcgccc
     1081 ctgggcctca cccgtgtggt acgcggccga agggtacgag catttctact gggtctcgtc
     1141 tccggaggcg agacactcac gcaacgtctc tgcgcgtccc gaggtaggca tcgtgatctt
     1201 cgactcccgg gcacccgtcg gcacggggca gggggtgtac gtgtcggcaa tcgccgagga
     1261 gcttgcgggc accgaactcg accggggcat cggtgtctac tctcgcagat ctcagacaca
     1321 cggagcaggc gggtggacac gggaggacgt aacgccgccg gcccgccacc gcctgtatcg
     1381 cgcgaccgca tctgagcact ctgtacttga cgataaggat cgtcggacgc tggtgagagt
     1441 gtaggcgcgg cgtgactggt gaagccaggt cagcctgaag atgctcctcg atgtcgtcaa
     1501 cgtgccagta ggcgacggga cccgtcatcc cttgctgtgg ccgtggggat ccagaccaat
     1561 gtcctggcct tcgacgttga agccgacgta gtaggcttcg tccatgtagg gctcgacgcc
     1621 caacagctcc ccgtacagcg tcttggcgtt ggcgaggtcc gtaacggggt acatgatcgt
     1681 cttgacgtct tcagtcatta gatactcctt gtactcgtgg tgtactcatt gttgctcgct
     1741 gtcgagttgg gctcgttcag atccacctcc ccggtttgtc cttctgtact atggcgctgc
     1801 ctggcgccgc tcggccttgg gctccaccac ctgccattta ggctgatacc ggacgttatg
     1861 gtgagttcct cacgctcgtt gcgttacctt ggcctggttt ggttcgtcat tacaataacg
     1921 gacagcgatg gaggaaggta acagtggaag aacaagactg gctggcacag cggttcgaga
     1981 cacatcggac tcgcctgcgg gcggtggcct acaggatgct cggctcgctt tccgaggcgg
     2041 aagacgccgt gcaggaggcc tggttccgcc taagccgctc tgagaccagc ggcatcgaga
     2101 acctgggcgg atggctgacc acggtcgtcg gccgggtgtg cctggacatg ctgcgctcac
     2161 gcacctcgcg ccgcgaggag catctggagg agcctcaggc tgcgcgcgtg tccgagccta
     2221 tattgggccg cgatgccggg gccgaccccg agcacgaggc actactagcc gactcggtcg
     2281 gtctggcgct gctggtcgta ctcgaaatgc tctctcccgc cgagcggctc gctttcgtgc
     2341 tgcacgacat gttcggggtg ccctatgagg agatcgcccc gattgtggac cgttctccga
     2401 acgcggccaa gatgctcgca agccgggcgc gccgccgggt gcggggcaag gcgacggtcc
     2461 ccgacgcaga tctcaccctc cagcgggagg tcgtcgacgc tttcctcgcc gcctcgcgag
     2521 gcggcgactt cgaggcgctg ctgaatgtgc tcgaccctga cgtcgtgctt cgtgccgaca
     2581 gcgcagccgt gcaagctggc gcagcgaacg aggtccgcgg cgcgccggcc gtagccgaca
     2641 ccttctccgg ccgcgcccgg ttcgcacgac cagcgctcgt aaacggagcc gccggagccg
     2701 tgtgggcgcc gggcggacga ccacgcgtcg tcttcggctt caaagtaacg ggcgggaaga
     2761 tcgtggagat caacctgatc gccgaccccg cacgtctccg ccagctcgac ctggctgttc
     2821 tcgacggctg aggcgaaact cgggcactca tcgccagcct atggtccatg gctacgggtc
     2881 cgcagtcctc tctatatccc ttgccgctcc tacgaccagc tgggagagcc gctggcccgg
     2941 actcaccccg aggcgtgaga tcggaccgct gacgcttacc gccgccctga gcctcccatc
     3001 ctccccgaat acgggcgcgc tgacgcttgc taccccggcc tcccgctcgg ccacgctctc
     3061 agcccagcct ctgactcgga tccctcgcaa ctgggccgca tccggcctgc catcgactcc
     3121 tccctgcgcc caggcgagca agactttccc cgccgatcct ctcgagagcg gcatgacagc
     3181 cccaactggt acagtgtccc taaggccggt cacccgctcc acagcggcca cgcacaccct
     3241 ctgctcgccc tcccgcacgt agagctgcgt gctctcccca ctctcatccc gcaacccttc
     3301 cagcacagga cgcgcccgct ccacgagacc taccccagct gccctgttcc cccaccctag
     3361 tagcctcaag ccaaggacgt atcgtccgtc ccgccgcgac acaaggtggt gcatctccag
     3421 agccgatagg agccggtgcg ccgtgggacg ggacaagctg gtcccctcga ccacctcaga
     3481 tagcgacgcc gagccatccc ccgaaaggaa agccaggata gccacagcct tgtccaacac
     3541 cccgacccca ctcttgcttc cggaagaaac tatttctata ctgtccatat gtcggtcatt
     3601 atgtctcaca ttttgagata tagcaagaag gtgtgatcct ggctcgcgag tgggacgcag
     3661 gcgaatatga gaagctctcg gcgccgcaga cccgttgggg ggttgaggtc ctcgagcgtc
     3721 tggtggtgcg cggagacgag gctgcgatcg acgcgggttg cgggactggt cgggtgacgg
     3781 agctattgct ggcgaagttg cccgaggggt ctgtgctggc tatagacggt tctcgggcga
     3841 tggtcgaggc ggcgaggagg cggtttgcgg gagagccgag ggttcgggtg gagctccagg
     3901 atctgttatc gctcgatgtg gtggagtgtg tggatctgat cttctccacg gcaacgttcc
     3961 acttgatatg ggatcatgac cacctcttcg agaacctggc acgtgccata aagcctggtg
     4021 ggcgcctcgt cgcccagtgc gggggcgagg gtaacatctt ccgtgccacg agggcgacga
     4081 aggagacgat ggaagaggag cgctttttgg actatttcgt gggctggcag gacgacaagt
     4141 actacgctga cgcccagagc acggcgagac gcctgaaagc cgcaggcttc gaggatgtcg
     4201 agacctggct gcacgacgag gttgcggcgt tcgattcggt ggacgagctc gcccgcttcc
     4261 tcgggacggt cgtgcttggt gggcacctgg agatgctgcc tgaggaggag cggtcccctt
     4321 tcgcagcgag ggtggcggag aaggtggcag ccgtggacgg ctcgcccacc cttgactacg
     4381 ttcgtctcaa catcatggca cgcaggtccg cgttgtccct cgaaggagaa ggtactaaca
     4441 ttacgagtcc cgcgactgag ccgccgggag ttgcagagac ggaggaggaa gcatgagcgg
     4501 tccgaggacg cttgtagaga aggtgtggga gaagcatgtg gtaaggagcg ccgaggggga
     4561 gcccgacctt atctacgtcg acctgcacct ggttcacgag gtgacgagcc cccaggcttt
     4621 cgagggcctc aggctcgccg ggaggggggt gcgccgtccc gagctgaccc tggcgacgat
     4681 ggaccacaac gtcccgactt cggagttcgg cctgcccatc gaagacaagg tctcggcgaa
     4741 gcagatggag gctctgagaa acaactgtga ggagtttggc atcaagctca acgagtgggg
     4801 gtccatcggg cagggtatcg tgcacattat cgggcccgag acgggcctca cccagcccgg
     4861 actcgtgatt gtctgcggcg actcccacac agccacccac ggtgcgttcg gcgcgcttgc
     4921 tttcggcatc gggacgagcg aggtcgagca cgtcctcgcc acccagacgc tcacccagag
     4981 caggccgaag actatggccg tcaccgtcga gggcgagctt cccgcggacg tcacggccaa
     5041 ggacctcatg ctcggcatcc tgaaccgcat cgggacgggt ggtggggttg ggcacgtcat
     5101 agagtacagg ggtgaggccg taaggcagct ctcgatggag ggtcgcatga ccgtgtgcaa
     5161 catgtccatc gagggcgggg ccagggcagg cctcgtggcg ccggacgaga caaccttcga
     5221 gtacctggaa ggcagggagt acgccccgaa gggcgaagat tgggaaacag cggtcgagga
     5281 atggaagaag ctcccgaccg acgagggcgc cgagttcgac aaggaggtcg tgatccgggc
     5341 ggaggacctg gtcccctacg tctcctgggg cacgaccccg gcacagaccg ttccgctcga
     5401 cggcgaggtc ccagagccgc agaacgaagg ccacgagagg gcgctcaagt atatggcact
     5461 ccaaccgggc acccccatcc gggacataga agtagatacc gtcttcctcg ggtcgtgtac
     5521 caacgcccgc atcgaggacc tgcgcgccgc cgccggcgtt ctggagggcc acaaggtcaa
     5581 ggaaggcata agggcgatgg tcgtcccagg ttccatgcgt gtcaaaaagc aggcagaaga
     5641 agaaggtctg gccgagatct tcaccgaggc aggcttcgac tggcgcaacg cgggatgctc
     5701 gatgtgcctc ggcatgaacc ccgacatact acagccggga gagcggtgcg cttcgacctc
     5761 gaaccgcaac ttcgaggggc gccagggcaa gggtggcaga acgcacctgg tcagtcccgt
     5821 ggtggcagcg gcgacggccg tgatgggcag gttcgcctcg ccttcggaac tcggcgtacc
     5881 actggaggtg ggctagtgga gcctgtcgag cgcatctctg ggacggcgct tccgctcggt
     5941 tattcggatg tggacacgga ccagatcgtc cctagcgacg ccctcaagcg catcgagcgc
     6001 accggtttcg gtcagttcct gttctccggc tggcgcgagg acgaagactt cataatgcac
     6061 agggaagagc acaaagacgc tgtgatactt atcgcgggcg agaacttcgg ctccggttcc
     6121 ag
//
LOCUS       DATMJA010000425         5094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_294389, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000425 DATMJA010000000
VERSION     DATMJA010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294389"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VNT12_10135"
     CDS             complement(<1..1179)
                     /locus_tag="VNT12_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWI46518.1"
                     /translation="MTTGQSSRSSRTKEPSATDHQEIEWQFDASELEPVEGWLDQHNS
                     GSSGLFIAPESTIEITDTYYDTDDWRFYRAGYALRVRNTAGAVEATMKSLTPAEGSLR
                     RRREISEPLEDDKPSTLKQAGGAVGERSRTLVGGREMRPLFTLETRRQGFALLLEGST
                     DRNQEDVRIGEISLDTSKIPLGEVTARLTRVEVEAGIGMAPTPELLDFVDEMQSALEL
                     TPASISKYEAGLYASGLNPKGDSEFGPTHVDPSMNLGEVAFAVLRTQFAEMQNHEPGT
                     RLGEDPEELHDMRVPTRRMRAAMKVFEEALPERASWLRDELRWVARALADVRDLDVQI
                     ERFQTWQEEADEESFGFLDRILTITHKRRAEARKNMLETLDSVRYERLLSSFAEMLRL
                     G"
     gene            complement(1294..3369)
                     /gene="ppk1"
                     /locus_tag="VNT12_10140"
     CDS             complement(1294..3369)
                     /gene="ppk1"
                     /locus_tag="VNT12_10140"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HWI46519.1"
                     /translation="MTDVARKPSLSDPSLYINRELSWLGFNNRVLEQARDERHPLLER
                     VRFIAISETNLDEFFMIRVAGLQQQVASDLPNPVPDGMTPEEQLSRIHDHTEEFFEER
                     RRILNQQLMPALEEEGIRTVPHGKVRAAERRELRERFVRDILPILTPLAIDPAHPFPH
                     ISNLSLNLLVVIEDAGRRVMARVKVPTTIDRFMRLADENRGPGERPEMRLVRVEEVIS
                     ANLDELFPGKEVVASYVFQVTRNADFVIEEDEAADLLQAIEDELEGRWFGQSVRLVVS
                     DEIPDDLRDWLAGNLKLDENAVFASPHPIGLADVEELTHIDRSDLLYPPITPRIPSEI
                     RSARSITQAIRNGDILLYHPYDSFAPVVDFVRAAANDPEVLAIKQTLYRVGSKSPIVE
                     TLSEARDEQTQVAVLVELKARFDEEPNITWARQLEARGVHVAYGIVGLKTHAKISLVV
                     RREGSGLRRYVHLGTGNYNPGTARVYTDFSYFTDEQKLAEDGSDLFNYLTGYSEQEEY
                     QELLVAPLTMRESIVRLIEEQTEKAKGGEPASITCKTNSLTDPQIIELLYEASQAGVK
                     IDLIVRSICCLRPGVEAVSENIHVVSLVGRFLEHARIFAFSDGDDEKMYLGSADLMQR
                     NLDRRVETAFPLREQRHREKVRRLLDLQLADNANGWELSPDGTFERLHPKDGEEYVDS
                     QAILLEEGF"
     gene            3646..3963
                     /locus_tag="VNT12_10145"
     CDS             3646..3963
                     /locus_tag="VNT12_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWI46520.1"
                     /translation="MKVRDYDAHVLVCKGGDCKKRGSKAVRKSLKDELRAESMNRDVR
                     VNSVDCLGLCKHGPNLVVYPGGTWYLGVVEQEVPEVVQRHLKNGEPVEHLAAELRSRR
                     RAR"
     gene            complement(4075..4656)
                     /locus_tag="VNT12_10150"
     CDS             complement(4075..4656)
                     /locus_tag="VNT12_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWI46521.1"
                     /translation="MRTYRMRRSGLRMFGYLVATLLVVSGLVLVGYSFLIRDPFASAA
                     VFAQQPPSNATMTLTVPEMRRVDHVPVYTGAANDKGALHDGTLHLKGTGFPWQTGANV
                     YIAGHRMGFPGTRSYLVFWDLNKLEDGDKIILTDANGTRYTYSVFKSIIINPDASYIT
                     RPVPGKSIISLQTCTLPDYAQRLIVQAELTDVS"
     gene            complement(4781..>5094)
                     /locus_tag="VNT12_10155"
     CDS             complement(4781..>5094)
                     /locus_tag="VNT12_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWI46522.1"
                     /translation="NWVYAWQRTANVYIAGHRVGYPGTKSNLVFWDLDKLEKGDEIFL
                     ADADGTRYTYEVFEKRVISPNTVSIMRPTKGKNIISLQTCTLPDYSHRLIVQGELKGE
                     K"
BASE COUNT          909 a         1532 c         1587 g         1066 t
ORIGIN      
        1 gccgaggcgt agcatctccg cgaaagagga caaaagacgc tcgtaccgga cggagtcgag
       61 cgtctccagc atgttcttgc gggcttccgc ccgccgcttg tgggtgatgg tcaggatcct
      121 atccaggaac ccgaagctct cctcgtccgc ctcttcctgc caggtctgga agcgctcgat
      181 ctggacgtcc agatccctta cgtcggcgag agcgcgggct acccagcgca actcatccct
      241 gagccagctg gcccgctcgg gcagtgcttc ctcgaagacc ttcatggcgg cccgcatccg
      301 gcgcgtcggc acgcgcatgt cgtgcagctc ttcagggtct tcgccgagcc gcgtgccagg
      361 ctcgtggttt tgcatctcgg cgaactgagt gcgcagcacg gcgaacgcga cctcgcccag
      421 gttcatggaa gggtctacgt gcgtagggcc gaactcggag tctcctttcg ggttgagccc
      481 gctggcgtag agaccagcct cgtacttgga gatagaggct ggtgtcagtt cgagggccga
      541 ctgcatctcg tccacgaaat ctagcagctc gggggtcggg gccatgccga tcccggcctc
      601 tacctccaca cgagtcaagc gggccgtcac ctcgcccagg ggtatcttcg aggtgtcgag
      661 cgagatctcg ccgatgcgca cgtcctcctg gtttctgtct gtagaacctt cgagcaggag
      721 cgcgaaaccc tggcggcggg tctcgagcgt gaacagtggg cgcatctcgc gcccgccgac
      781 gagcgttcta gatcgttcgc cgaccgcccc accggcctgc tttagcgtgg agggtttatc
      841 gtcctcgagg ggctcggaga tctcacgccg cctcctgagg ctgccctcgg ccggcgtcag
      901 ggatttcatc gtggcctcga ctgcgccagc cgtgttgcgg acgcgcaggg cgtagccggc
      961 cctgtagaaa cgccagtcat cggtatcgta gtaggtatct gtgatctcaa tcgtggattc
     1021 aggggcgatg aagagacccg aagaacctga attgtgctgg tcgagccagc cctcgactgg
     1081 ctcgagctcg ctggcgtcga actgccactc tatctcctgg tgatccgtcg ccgaaggctc
     1141 tttcgtacgg gatgatctgc tgctctgtcc cgttgtcatt cgtaccccgt ttcgttagtc
     1201 cgtaccagca gcgttccccg aaagacagag gcgcaatcta gcacggacct ggatcactgg
     1261 caacaaacgt gtatatggcg gtatctccga tgattagaag ccttcttcga gcaatatcgc
     1321 ctggctgtct acgtattcct caccgtcttt gggatgcagg cgttcgaagg tgccgtcagg
     1381 gctgagttcc cagccgttcg cgttatcagc gagctggagg tcgagcaggc ggcggacctt
     1441 ctcgcggtga cgctgctcgc gcagcgggaa ggccgtctcg acgcgccggt cgaggttgcg
     1501 ctgcatcagg tcggcgcttc cgaggtacat cttctcgtca tccccgtcgg agaaggcgaa
     1561 gatacgggcg tgctcgagga agcgtcctac cagcgagact acgtggatgt tctcgctcac
     1621 ggcttccacg ccgggcctga ggcagcagat gctgcgtaca atcaggtcta tcttcacccc
     1681 ggcctgggag gcttcgtaga ggagctcgat gatctggggg tccgtgagcg agttggtctt
     1741 gcacgtgatg ctggcgggct cgccgccttt agccttctcg gtctgctcct cgataaggcg
     1801 cacgatgctc tcacgcatgg tcaggggggc gacgagtagc tcctggtact cttcctgctc
     1861 ggagtagccg gtcaggtagt tgaagaggtc tgagccgtcc tcggcgagct tctgctcgtc
     1921 ggtgaagtac gagaagtccg tgtagacgcg ggccgtcccg ggattgtagt ttcctgtgcc
     1981 aaggtggacg tagcgtctta gtcccgagcc ctcgcgcctg acgaccaggc tgattttggc
     2041 gtgcgttttg aggccgacga taccgtaggc gacgtggacg ccccttgcct cgagctggcg
     2101 cgcccaggtg atgttcggct cttcgtcgaa gcgtgctttc agctcgacca ggacggcgac
     2161 ctgtgtctgc tcgtcgcgcg cctcagagag cgtctcgact atcggggact tcgagccgac
     2221 acggtagagc gtctgcttta tggcgagcac ctcggggtcg ttggccgccg ccctcacgaa
     2281 atcgactacg ggcgcgaacg aatcgtaggg atggtagagc agaatgtcgc cgttcctgat
     2341 ggcctgggtg atggaccgcg cgctccggat ctccgaagga attcttgggg ttatcggggg
     2401 atagagcaga tcggagcgat cgatgtgcgt gagctcctca acatcagcga gccctatcgg
     2461 gtggggagag gcgaagaccg cattttcgtc gagcttgagg ttgccggcca gccagtctct
     2521 caggtcatcg gggatctcat ctgagaccac gagcctgacg ctctggccga accagcggcc
     2581 ctccaactcg tcctctatcg cttgcaggag gtcggccgcc tcgtcctcct cgatcacgaa
     2641 gtccgcgttg cgggttacct ggaagacgta gctcgcaacg acctctttgc cggggaagag
     2701 ctcgtccagg ttcgccgaga taacttcttc gaccctcaca aggcgcattt cgggcctttc
     2761 gccgggaccg cggttctcgt cggcgagccg cataaagcgg tctatggtgg tcggcacctt
     2821 tacgcgggcc atcacccgcc ttcctgcgtc ctcgatcacg acgagcaagt tcaatgatag
     2881 gttggagatg tgggggaatg ggtgcgccgg gtctatggcg aggggcgtga gtatgggcag
     2941 tatgtccctg acgaaccttt ccctgagctc ccgccgctcg gcggctcgga ccttgccgtg
     3001 aggcaccgtg cgtataccct cttcttccag agcgggcatt agctgctggt tcagtatcct
     3061 gcgccgctcc tcgaagaact cctccgtgtg gtcgtgtatc cgggagagct gctcttctgg
     3121 cgtcatcccg tctggaacgg ggttcgggag atccgaagcg acctgctgct gcaaccctgc
     3181 gacccggatc atgaaaaact cgtcgaggtt ggtctcggag atcgcgatga accgcaccct
     3241 ctccagcagc gggtgtcgct cgtcgcgggc ctgttcgagg accctgttgt tgaaccccaa
     3301 ccacgagagt tccctgttta tgtacagcga agggtcggaa aggctcggct tgcgtgctac
     3361 gtccgtcata tctcagcccc ctcctcgagc agcgtcccgt ctccgactcc tgcacgcgcc
     3421 acggtcagac cgccggcccc ataatatgtc gtgaaatctc ataccgccca tgataccgtc
     3481 ccaccggctc gggtgatata ttcgaacctc ccacgtgggt tgtcgatcgc acccgcacgg
     3541 cgcaagccgg cgtaagaacc ggaggaagcc atctcgaaga gaggcaagaa gacgaagaag
     3601 aaagcacaga agggtggcgc gctggcgacg ctcgcaaagc cggccgtgaa ggtcagggac
     3661 tacgacgccc acgtgctcgt ctgcaagggt ggcgactgca aaaagcgcgg gtccaaagct
     3721 gtgcgcaagt cgctcaagga cgagttgcgc gcggagagca tgaacaggga cgttcgcgtg
     3781 aactccgtag actgcctcgg cctctgcaag cacggtccga acctcgtggt ctacccaggg
     3841 ggtacctggt acctcggcgt cgtagagcaa gaagtgcccg aggtagtaca aagacaccta
     3901 aagaacggcg aacccgtcga acatctcgcc gccgaactga gatcccgtag aagggccaga
     3961 tagcggcggg tgtaaggctc gagccttaca cccgccgagc acttcagcag gtcagctttt
     4021 tgtttgctga agtgctgatc agcctgctgt gcggaattct actgccgcgc agcgctaact
     4081 tacgtctgtc aactcggcct gaacgatcag gcgctgcgca tagtcgggca gcgtgcacgt
     4141 ctgcaggctg atgatgctct tgccggggac cggccttgtg atgtacgagg catcggggtt
     4201 gatgatgata ctcttgaaca cgctgtaggt gtacctggtg ccattcgcgt cggtaaggat
     4261 gatcttgtcg ccgtcctcta gcttgttcag atcccagaac acgagataac tcctggtgcc
     4321 tgggaagccc atgcggtggc ccgcgatata cacgttggcc cccgtctgcc aggggaaacc
     4381 cgtgcccttc aggtgtagcg tcccgtcgtg gagcgcaccc ttgtcattcg cggcgcccgt
     4441 gtagacgggc acgtggtcga cacgccgcat ctcggggacg gttagggtca tagtcgcgtt
     4501 gctcgggggc tgctgcgcga agacggccgc gctggcgaac gggtcgcgta tgaggaagga
     4561 gtaccccacc agaaccaacc cggacacgac gagcaaggtg gctacgaggt agccaaacat
     4621 cctgagcccg cttcttctca tcctgtaggt tctcacgtcg atctacctcc tctccgtaga
     4681 ttcagatctc ttggctgtct aggtatacac ggtgaaagta acattcaggt tacaaaatgg
     4741 tccctacgat gtcaatcgag cacatagtcc tgctaggagc ttacttctcg cccttcagtt
     4801 cgccctgcac tatgaggcgg tgcgagtagt cggggagggt acaggtctgg aggctaatga
     4861 tgttcttacc ctttgtgggc cgcatgatgc tgacggtgtt gggtgagatc acacgcttct
     4921 cgaatacctc gtaggtgtaa cgcgttccgt ctgcgtccgc gaggaagatc tcatccccct
     4981 tctccagctt atcgaggtcc cagaacacga ggttgctctt cgtccccgga taaccaaccc
     5041 tgtggcccgc gatgtagacg ttggcagtcc tctgccacgc gtagacccag ttct
//
LOCUS       DATMJA010000426         5396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_294525, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000426 DATMJA010000000
VERSION     DATMJA010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294525"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VNT12_10160"
     CDS             <1..1001
                     /locus_tag="VNT12_10160"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWI46523.1"
                     /translation="GDSEFGPTHVDPSMNLGEVAFAVLRTQFAEMQNHEPGTRLGEDP
                     EELHDMRVPTRRMRAAMKVFEEALPERASWLREELRWVAHALGNVRDLDVHIERFQAW
                     QEEADEESSGFLDRILSITHKRRAEARKNMLETLDSVRYERLLTSFAEMLRLGPAAEL
                     ELAQTNGKDQAGEPVTAAAPALISDRYRKWRKAAKRLDETSSPEAFHDVRKKGKRLRY
                     TLEFVSEVYGRSVQKLVKPLKALQDDLGDHQDAVVAAAYLRELGTTTGQARIPRGVAF
                     TMGIYAERCTREAKDLRSDVPGSKPFRTLTKGKKWKKFEKVLESQREANIPNKRVR"
     gene            1008..1514
                     /locus_tag="VNT12_10165"
     CDS             1008..1514
                     /locus_tag="VNT12_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWI46524.1"
                     /translation="MYVYLIRHAVAHKRDQERWPDDSRRPLTPEGEEEFQVAARGLAR
                     VVPEVELLLSSPYERAWRTAEILAEQAGWPVPEKHPALEPDVPPEKVVMALQTYQGKQ
                     SIALTGHRPCLPELAVYMLTSDVGGADMKIKKGGVVCIEFDDAPKAGAGKLRWLFTPK
                     VLRNMAVS"
     gene            complement(1682..2341)
                     /locus_tag="VNT12_10170"
     CDS             complement(1682..2341)
                     /locus_tag="VNT12_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HWI46525.1"
                     /translation="MEKYDRVWKRLVGERRLEFGGYMDPDWQEGHRLSASLIVPGEAS
                     RFHERLEPLREALRPFPFVSLHPDHFMHVTLVLLGFLVDEPREEDEISRERLREIEND
                     ARIVLSDVPHFTVTLANLNAFPGAAFIEAHDGGKLDNLRDALCAGCGLKKPPGPPHLT
                     IAYFQAPDGTDAPEELISTIARYRDWPIGEILVEHVELTLLDLRSEYPEPETLVQIPL
                     G"
     gene            complement(2527..3600)
                     /locus_tag="VNT12_10175"
     CDS             complement(2527..3600)
                     /locus_tag="VNT12_10175"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HWI46526.1"
                     /translation="MVGRTGPILSAYVSVNADIPENQGRAYLVRLRDAMNDEGVPEGL
                     RRRVREYTEKETHPEARTLAIFADEDDLFEVYRLQVDVPESFRWGDPHVAPLTLVLDE
                     YEPYGAAILDAERFRYFVVSPLSTAHGEEEIKANGFREVDVHPSEPYPRGGTDFEAAS
                     RRTEAHVHRFYKELGELTRDVTLHEGVQRLILAGPRERTAEFRRTLPNELHDRVVAEE
                     HVDLGLPEGELLDRLEAIRERAEHEREKDLLDEIRESGVRGLDETVAALQEENRVYHL
                     AALWELDAEIRWCDNDQLAIREATREECPFCGQQTRVRPLTDVLVDLAAARGARLDFM
                     RGENENTDTLRDEFGGIAGLTRF"
     gene            complement(3739..4119)
                     /locus_tag="VNT12_10180"
     CDS             complement(3739..4119)
                     /locus_tag="VNT12_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWI46527.1"
                     /translation="MQKDLVKSEPLSGIEVGRESEEMCLLAKLFNGFANSTRLSILLL
                     LAQRGEMKVGALVDELGAPQPRVSDHLRCLAWCGYVQVRREGRNAFYAVADERVMQIL
                     KLGEELLQDNLEHVESCDDMEKGC"
     gene            complement(4315..>5396)
                     /gene="solA"
                     /locus_tag="VNT12_10185"
     CDS             complement(4315..>5396)
                     /gene="solA"
                     /locus_tag="VNT12_10185"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWI46528.1"
                     /translation="GERVLGLERHTPAHDKGSSHGQSRIIRLAYSEDPAYVPLVLRAY
                     ELWERLERETGQDLMTLTGGLMIGPPGTKFFEGSKESAERYDLPYEVLDAAELKRRYP
                     VFEPTPDTVALFEEKAGFLRPEACVKAHLNRAASLGADLRFEEGLLSWEPTESGVLVE
                     TASGAYEAERMVVSSGAWAPKLLADLGLPLEVTRQLLFWFDPKGSIESFLPDRFPIFI
                     WEPEDGNSFYAIPAHDGPRGGVKVAFFRADGKPADPETIDRKVHDEEVEFIRSYLARY
                     VPDLDGDFLYAKTCMYTNTPDEHFVISAHPKYPQVAIAAGFSGHGYKFCSVVGEILAD
                     LATVGETPHPIDLFSTARLLGTARG"
BASE COUNT         1003 a         1721 c         1668 g         1004 t
ORIGIN      
        1 aaggggactc cgagttcggc cctacgcacg tagacccttc catgaacctg ggcgaggtcg
       61 cgttcgccgt gctgcgcact cagtttgccg agatgcaaaa ccacgagcct ggcacgcggc
      121 tcggcgaaga ccctgaagag ctgcacgaca tgcgcgtgcc tacacgccgg atgcgggccg
      181 cgatgaaggt cttcgaggaa gcgctccccg agcgggccag ctggctcagg gaggagttgc
      241 gctgggtagc ccacgctctc ggcaacgtaa gggatctgga tgtccacatc gagcgcttcc
      301 aggcctggca ggaagaggcg gacgaggaga gctccgggtt tttggacagg atcctgagca
      361 tcacccacaa gcggcgggcc gaagcccgca agaacatgct ggagaccctc gactccgtcc
      421 ggtacgagcg tctcttgact tctttcgcgg agatgctgcg cctcggccca gctgccgagc
      481 tggagctcgc acagacgaac ggcaaagacc aggcgggcga gccggtcacg gcagcggcgc
      541 cggccctgat ctcagaccgt taccgaaagt ggagaaaggc ggcgaagcgt ctcgacgaga
      601 cctcatcccc cgaggccttc cacgacgtac gcaaaaaggg gaagcggttg cgctacaccc
      661 tggagttcgt ctctgaagtc tacggtagat cggtgcagaa gcttgtcaaa cctctaaagg
      721 cactacagga tgatctcggc gaccaccagg acgccgtggt cgccgccgca tacttgaggg
      781 aactcggcac aacgaccggc caagcgcgga tcccccgcgg ggtcgccttc acgatgggca
      841 tctacgcaga acgctgcacc cgtgaggcga aggacctacg ctccgacgta cccggctcga
      901 aacccttccg caccctgacc aagggcaaga agtggaagaa gttcgagaag gtcctggaga
      961 gccagcgtga ggctaacata ccaaacaagc gtgttcggta ggcccgcttg tacgtctatc
     1021 tcatccgcca cgccgtcgcc cacaagcgtg accaggaacg ctggcccgat gacagcaggc
     1081 gtccgctcac gcctgagggc gaggaagagt tccaggtggc ggcccgcggg ttggcccgtg
     1141 tcgtccccga agtagagctc ctcttgagca gcccctatga acgggcgtgg cggaccgcgg
     1201 agattctggc cgaacaggcc ggctggccag taccggagaa acaccccgcc ctggagcccg
     1261 acgtcccacc agaaaaagtc gtcatggccc tacagaccta ccaggggaag cagtccatag
     1321 ccctgaccgg gcatagaccc tgcctgcccg agcttgctgt gtacatgctc accagcgacg
     1381 tcgggggcgc agacatgaag ataaagaagg gcggtgtcgt gtgcatcgag ttcgacgacg
     1441 ccccgaaggc cggagccggt aagctgcgct ggctgtttac cccgaaagtc ctccgcaata
     1501 tggccgtcag ctaggcagca cgctccggct ggcgcctccg cttgtcagct cggcggctgc
     1561 gccgctttct cagccggtcg gcacgcctac ttcgcaggct tgtcagccac tcagccaggg
     1621 cttttgggca cacgcaggct gaagtgctga caagcgcccg aaagggtgcg tgctgcctag
     1681 ctcaccccaa cggtatctgg accagcgttt cgggctccgg gtactccgag cgaaggtcga
     1741 gaagggtcaa ctccacgtgc tccaccagga tctcgccgat gggccaatct ctgtagcgtg
     1801 cgatggtgga gatgagttcc tcgggcgcat ccgtgccgtc cggcgcctgg aagtaggcga
     1861 tggtcaggtg cggcggacca ggaggcttct tcaagccgca gcccgcgcac agagcgtcac
     1921 gcaggttgtc gagcttaccg ccgtcgtgcg cttcgatgaa cgccgcgccg gggaaagcgt
     1981 tgaggttcgc gagcgtgacg gtaaagtgcg gaacgtcgga cagaacgatc ctggcgtcat
     2041 tttcgatctc tcgcaacctc tctcgggaaa tctcatcctc ttcccttggc tcgtccacga
     2101 ggaagccgag aagcaccagc gtaacgtgca taaagtggtc ggggtgcagc gacacgaacg
     2161 ggaaaggacg cagtgcctct ctcagtggtt cgaggcgctc gtggaaccgt gaagcctccc
     2221 cagggactat gagggaggcc gagagcctgt gtccctcctg ccagtcgggg tccatgtacc
     2281 caccgaactc cagacggcgt tcaccaacga ggcgcttcca cacccgatca tacttctcca
     2341 tccacgaact ctagcagagt gggcatccgc cccgctcctt gatgcagcat cggaatcgcg
     2401 ccttcgcagc tacagcactt tccggatctg tggaccatca aggagataca catcgccttc
     2461 tggccctcaa ctctgttccc aacctgaagc cgcgcttttg gaaggtcgac cttccaaaag
     2521 cgcggcttag aatcgggtca agcctgctat gcctccgaac tcgtcgcgca gggtgtccgt
     2581 attctcgttt tcgccgcgca tgaagtcgag gcgcgcgccg cgggcggcgg cgaggtcgac
     2641 gaggacgtct gtgagtggcc gcacgcgggt ctgctggcca cagaatgggc actcttcgcg
     2701 tgtggcttcg cgtatggcga gctggtcgtt gtcgcaccaa cggatctcgg catccagctc
     2761 ccagagggcg gcgaggtggt agacacggtt ctcctcctgg agggctgcga cggtctcgtc
     2821 gaggccgcga acgccgctct cccttatctc gtctaggagg tccttctcgc gctcgtgctc
     2881 ggcgcgctcc ctgatggcct cgagccggtc caggagctcg ccctcgggta accccaggtc
     2941 tacgtgttcc tcggcgacta ccctgtcgtg tagctcgttg ggcagggtgc gacggaactc
     3001 ggcagtcctc tctctggggc cggcgaggat cagacgttgc accccctcgt gcaaggtgac
     3061 atccctggtg agctctccca gttccttgta gaacctgtga acgtgggcct ccgttcggcg
     3121 gctggccgcc tcgaagtccg tcccacctct tgggtaaggc tcgctgggat ggacgtcgac
     3181 ctccctgaag ccgttggctt ttatctcctc ttctccatgg gcggtactca ggggcgagac
     3241 cacaaagtac ctgaacctct cggcgtcgag gatcgcagcc ccatatggtt cgtactcatc
     3301 aagtacgagc gtcagcgggg ccacgtgagg gtctccccag cggaacgact cgggaacgtc
     3361 gacctgtaac cggtagacct cgaagaggtc atcctcgtcc gcgaagatgg cgagcgtcct
     3421 ggcctccggg tgcgtctcct tctctgtata ctccctgacc cgccgccgca gcccctcagg
     3481 gacgccctca tcgttcatgg cgtcacgcag tcggaccagg tacgcccttc cctggttctc
     3541 cgggatgtcg gcattgacgc tcacatacgc cgagagaatc ggtcccgtcc tgcctaccaa
     3601 ggaggtaatc gtctgtctga gatcctgagc ctgggccata cttgaaacac ctcttgcata
     3661 taccttgcaa ggactggccc tactagaatc cccgcccttc gagtggagta aacacgccct
     3721 ccggtcctag agcggagtct agcagccctt ctccatatcg tcacaggatt cgacgtgctc
     3781 caggttgtcc tgtagaagtt cctcgccgag cttgagtatc tgcatgaccc tttcgtcggc
     3841 cacggcgtag aaagcgtttc tgccctcgcg gcgcacctgt acgtaaccgc accaggccag
     3901 gcagcgcagg tggtctgaga ccctcggctg gggcgccccg agctcgtcta ccagcgcccc
     3961 gaccttcatc tcgccccgtt gcgccagaag cagcaagatc gatagcctgg ttgagttggc
     4021 gaaaccattg aacaacttgg cgaggaggca catctcctca ctctcgcgcc ctacttcgat
     4081 accggataag ggttctgact tgactaaatc cttctgcaca atttactcct tccaaattct
     4141 ttcctaatat acaccgcctg ttcgaacgtg tgaaccttat tacagcgggt cgggggaagg
     4201 gcggaccttc ttccgacccg cgcatttcag ccagtcagca cggcgccagg cttcgccagg
     4261 cggcttgtca gccagggcct gcgggcgaaa gctgacatgc aaggatctcc aaggctaacc
     4321 tcgcgcggtg ccgagcaggc gagcagtgga aaagaggtct atcgggtgtg gggtctcgcc
     4381 tacggtggcg aggtcggcca ggatctcacc gactacgctg cagaatttgt agccgtgccc
     4441 ggagaagcct gcggcgatgg cgacctgtgg gtacttgggg tgcgcggaga tcacgaagtg
     4501 ttcgtccggg gtgttcgtgt acatgcaggt cttggcgtag agaaaatcac cgtcgaggtc
     4561 ggggacgtac ctggcgagat agctcctgat gaactctacc tcctcgtcgt gaaccttgcg
     4621 gtctatcgtc tcggggtccg ccggcttgcc gtcggccctg aagaacgcga cctttacccc
     4681 gcctcttggt ccgtcgtggg cagggatggc gtagaacgag ttgccgtcct cgggttccca
     4741 gataaagatg gggaagcggt cggggaggaa cgattctatg ctgcctttcg gatcgaacca
     4801 gaacaggagc tggcgcgtaa cctctagcgg gagaccgagg tcagcgagaa gcttcggcgc
     4861 ccacgccccg gacgagacaa ccatccgctc cgcctcgtag gcccccgatg ctgtttccac
     4921 cagcaccccg gactcagtag gttcccagga cagcagtccc tcctcgaagc ggaggtcggc
     4981 gcccagggag gcggccctgt tcaggtgggc cttcacacaa gcctcgggcc tgaggaagcc
     5041 tgccttctcc tcgaagaggg cgacagtatc aggggttggc tcgaagaccg ggtagcgtct
     5101 tttcagttcg gcggcgtcga gaacctcgta gggcagatcg tacctctcgg cgctctcctt
     5161 gctgccctcg aagaacttgg tgcctggagg cccgatcatg agcccgcccg tgagcgtcat
     5221 caggtcctgg cccgtctccc tctccagccg ctcccacagc tcgtaggccc gaaggaccag
     5281 cggtacgtag gcggggtcct cggagtacgc caggcggatg atcctggatt ggccgtggct
     5341 ggacccctta tcgtgcgcag gggtgtgccg ctccagcccg agcacgcgct ctccgc
//
LOCUS       DATMJA010000427         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_354501, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000427 DATMJA010000000
VERSION     DATMJA010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354501"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /gene="rho"
                     /locus_tag="VNT12_10190"
     CDS             <1..901
                     /gene="rho"
                     /locus_tag="VNT12_10190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWI46529.1"
                     /translation="GQEPQKCRWRPNFDDLTPLYPMERLRLEWKPNDIAPRVIDLVAP
                     IGKGQRGMVVSPPKAGKTTILKQIAQSIAANYPETKLFVVLADERPEEVTDWQRSVEE
                     AEVVASTFDQPADNHIAVAELVLERVKRLVEEGEDVVVLLDGITRLSRAYNLAAPASG
                     RILSGGVDSAALYPPKKFFGAARNIENGGSLTILATALVETGSRMDEVIFEEFKGTGN
                     MELRLDRKMANRRIYPAINIEDSSTRKEELLMEPAEAQRVWQVRSILNALDTGQKIEL
                     LIQKLKETRTNPEFLRELQRVRG"
     gene            1011..1226
                     /gene="rpmE"
                     /locus_tag="VNT12_10195"
     CDS             1011..1226
                     /gene="rpmE"
                     /locus_tag="VNT12_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWI46530.1"
                     /translation="MKTGIHPEYTKTLVRCMCGNEFETRSTAGDTITVDLCSECHPFY
                     TGKQRIVDTGGRVQRFQNRRQARQNRQ"
     gene            1238..2152
                     /locus_tag="VNT12_10200"
     CDS             1238..2152
                     /locus_tag="VNT12_10200"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWI46531.1"
                     /translation="MRVGGQAIMEGVLMRSPNFWGMAVRTPSGDMDLRAERFRSVTRR
                     SKLFRLPIIRGALSLGETLWLGMKALTISTNIALGEEEELSKKEIAGTLIFALVLGLG
                     LFLVAPVLGTKGLGSFLGNSIENPVIFNLVEGFIRIAIFIAYLLGITLLSKDIKRFFA
                     YHGAEHKAIKVYERGEELVPANARKLDTSHVRCGTSFVLYVLVLSILVFSLLGVEGWL
                     YMLASRVIVIPLVAGIAFEFIMWSARHQDSALVRALVWPGLQMQKLTTREPSDDMVET
                     AMASLKKVLSMEKEAELEPNEATNKLVI"
BASE COUNT          453 a          671 c          693 g          395 t
ORIGIN      
        1 cggccaggag ccccagaagt gccgctggcg cccgaacttc gacgacctga cgcccctgta
       61 tccgatggag cggctgcgcc tggagtggaa gccgaacgat atcgccccgc gcgtcataga
      121 tctggtcgcg cccatcggca agggccagcg tggcatggtc gtctcgccgc cgaaggccgg
      181 caagaccacg atcctcaagc agatagccca gtccatagcg gccaactacc ccgagaccaa
      241 actcttcgtg gtgctcgccg acgagcgccc tgaggaggtc accgactggc agcgcagtgt
      301 agaggaggcc gaggtcgtcg cctcgacctt cgaccagccc gccgacaacc acatcgccgt
      361 cgccgaactc gtcctggagc gcgtaaagcg cctggtcgag gagggggagg acgtcgtcgt
      421 tctgctcgac ggcataacca ggctctctcg cgcctacaac ctcgccgctc ccgcttcggg
      481 aaggatcctc tccggtggtg tcgattcggc ggcgctctac ccgcccaaga agttcttcgg
      541 ggcagcgcgc aacatcgaga acggcggatc ccttaccatc ctggcgaccg cgctcgtcga
      601 gacgggaagc aggatggacg aggtcatctt cgaggagttc aagggcacgg gcaacatgga
      661 gctcaggctc gacaggaaga tggctaaccg caggatctac ccggccataa acatcgagga
      721 ctcctcgacg cgcaaggagg aactgctcat ggagccggcc gaggcccagc gggtctggca
      781 ggtccgtagc atcctcaacg ccctggacac ggggcagaag atagagctcc tgatccagaa
      841 gctcaaggag actcgcacga accccgagtt cctgcgtgag ctgcagcgcg tccgcggtta
      901 gcgactagat tcggacgggt gcgtaaacgc aatacacagc acccgtccct cgtctccttt
      961 acagacgtaa tagactatag gagacgatgt ttgagagacg gaggtatatg atgaagactg
     1021 gtatacatcc agagtatacg aagacgctgg tgcgctgcat gtgcggcaac gagttcgaga
     1081 ctcgctccac ggcgggggac acgatcacgg tggacctctg ctcggagtgc cacccgttct
     1141 atacaggcaa gcagcgcata gtggacaccg gcggtagggt gcagcgcttc cagaatcgcc
     1201 gccaggctcg ccagaacagg cagtagggcc gaacaatatg agggtcggcg gacaggctat
     1261 catggagggc gtcttgatgc gctcccccaa cttctggggg atggccgtcc gcacgccctc
     1321 gggggatatg gatctacggg ctgagcggtt ccgttccgta acccgccgta gcaagctatt
     1381 ccgcctcccc attatcaggg gtgccctctc tctgggagag accctgtggt tgggaatgaa
     1441 agcgctcacc atatccacga acatcgcgct cggcgaggag gaggagctct ccaagaagga
     1501 gatcgcgggc accctcatct tcgcgctggt gctaggtctc gggctcttcc tcgtcgcccc
     1561 cgtgctgggg accaaggggc tcggctcttt ccttggtaac agcatcgaga accctgttat
     1621 cttcaacctc gtcgagggat tcataaggat tgcgatcttc atcgcctatc tgctggggat
     1681 tacactactc agcaaggaca tcaagcgctt tttcgcctac cacggcgcgg agcacaaggc
     1741 gataaaggtc tacgaacgtg gcgaggaact cgttccggcg aacgcccgca agctcgacac
     1801 gagccacgtc cgctgcggga cgagcttcgt gctctacgtg ctagtgctct ccatcctggt
     1861 cttctcgctg ctcggggtcg agggttggtt gtacatgttg gccagccggg tgatcgtcat
     1921 cccgctcgtg gctggcatag cgttcgagtt catcatgtgg agcgcgcgcc accaggacag
     1981 cgcgttggta cgggcgctcg tgtggcctgg cctgcagatg cagaagttga ccacgcggga
     2041 gccctcggac gatatggtcg agaccgcgat ggcatccctg aagaaggtcc tctcgatgga
     2101 gaaggaagcc gagctcgagc ctaacgaggc taccaacaaa ctggtgatct gacttggata
     2161 gtcaggtagt aaagctggcc gaggagacgc tcggccgcta cgacgcgtta ac
//
LOCUS       DATMJA010000428         8999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_531618, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000428 DATMJA010000000
VERSION     DATMJA010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531618"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VNT12_10205"
     CDS             <1..311
                     /locus_tag="VNT12_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWI46532.1"
                     /translation="KLVGVELGGSNLGSYNDGSMIDYFPVYKNGERIGKVTSACYSPR
                     LDKSIGFAMVPVEYSEIGIELEIERPEETVPAVVADRVFFKPEHAEQELSATSSNKGA
                     "
     gene            415..1284
                     /locus_tag="VNT12_10210"
     CDS             415..1284
                     /locus_tag="VNT12_10210"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWI46533.1"
                     /translation="MNKTDKEQEVRDALAEALRRPRYEVIPLGGVEEAIVEHVPKSIK
                     LTVTASPKKGIEETLRLAERLSGRGYEVAPHISARLVRDEAHLEDVLGRLRRTGIKDA
                     FVIAGDADEPAGKFEGAAALLGTMAEIGHTLEEIGISGYPESHPLISDETTIQAMYDK
                     VPYATYIVSQICFDPALTAHWIRHVRNRGVELPIYIGIPGVVNTTKLMRISGSIGLGE
                     SARFLKKYGNRFFRMLLPGRYRPDRLIKDLTPSLTDPQCKVKGFHIYTFNEMGKTEAW
                     REKLETIEAAKTA"
     gene            1310..3013
                     /locus_tag="VNT12_10215"
     CDS             1310..3013
                     /locus_tag="VNT12_10215"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWI46534.1"
                     /translation="MQTFPSDLEIARQAKLKPLPEIADGMGIGEHLLEPYGDSVAKIK
                     LEAIEELEDRPLAKYVVVSAITPTPLGEGKTTTTVGLGQAFKHIGKRATVALRQPAMG
                     PTFGIKGGAAGGGYSQVVPMEMLNLHLTGDTHAVTAAHNLLAAMLDNHVHQNDDFFHI
                     DRHSVTWRRVLDVNDRSLRNIITGIGSRADGVPREAGFDITAASEVMAILALATSLRD
                     LRERLGRIVIGSTTDGKPVTAEALEAAGAMAVILKEAIKPNLMQTLENTPVLVHAGPF
                     GNIATGNSSIVADRIGIKGGDFLITEAGFGADMGAERFFNIKCRYSGLKADAAVLVAT
                     VRALKAHSGRFKIVAGRPLPPELLEENPDDVFAGADNLRKQIENVRLHGVSPVVAINA
                     FPTDHASEHAAIREIAEGMGARVAVCNHFAEGGAGAVELADAVAEATEEPTEFRFLYP
                     EEASLREKIETIATKVYGADGVAYDMKASQQLDSYENNGFGRLPVCMAKTHLSISSDP
                     TLLGAPKGWTLPVREVRASVGAGFIYPLCGEMRTMPGLSATPAATRIDLDDNGEVVGL
                     S"
     gene            3010..3618
                     /locus_tag="VNT12_10220"
     CDS             3010..3618
                     /locus_tag="VNT12_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HWI46535.1"
                     /translation="MTDAVPNYAELPLGRFVDMVASREPAPGGGASAAVAIALAAALT
                     AMAARFSADNLADAETIADRAEELRNKVMPLAQADAAAYGRVLDAYRTPRDDDEKRRR
                     RIREALSEAADVPLSIAEVGVEVVGNAARLVEEGNPNLRGDAMTAAALAKAGVRAAAT
                     LVEINVSAGGADDDRLSRVDQLLATMAAAQEVVEGAEARSWG"
     gene            3847..5151
                     /locus_tag="VNT12_10225"
     CDS             3847..5151
                     /locus_tag="VNT12_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HWI46536.1"
                     /translation="MTERRTPLYDFHFRSARNIVKGGGDFMFPTSYTSPVEEHLNVRR
                     NVGMQDLSTMGEVDIKGPGSERLVRRLLVNEVNDMEPGQLRYSTMCNEEGGIVDDVTV
                     YKFGDEHFMIVTSSGPRLKTYRWISKHAEGASAYATDVTAALALPVVQGPRSRTFLKT
                     LVEGVDLDALRFFRFAQCRIGEVELIISRSGYTGELGYELYTPADQAGPLWEYLLEKG
                     REFDLKPYGVEAMQSLRIEKSLPLYGPDINEEDTPFHVGLARWIRLEKRDLIGREALL
                     RIQERGLDRRWVGLTLESEVPAAAGAKVYSVGDVATFREVIETGAEAGEYEDAVMPGD
                     RQIGRVTSSAMGHSVGKMLAMAYVETAHSWPGNNLIVEVNGRPIRAKVAQTPYFDPEN
                     ARIRSEPAEDGRRSEPAPRLVATTDSGSNVPARQYTNGGPGS"
     gene            5148..6413
                     /gene="solA"
                     /locus_tag="VNT12_10230"
     CDS             5148..6413
                     /gene="solA"
                     /locus_tag="VNT12_10230"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWI46537.1"
                     /translation="MSVTYSGRYDAIVVGVGGMGSATSYYLARRGKRVLGLERFGIPH
                     SMGSSHGHSRIIRLAYYEHTSYVLLLKRAYELWREIQRIASERLLHITGSIDAGPEDS
                     WVFKGSWESCRLHDLPHEVLTGAELRRRYPGYHLPADHLALVQPEGGFLTPEKCIVSY
                     VMAAQALGAEIHAHERVFEWRPLEGGVRVRTDRGVYEADKLVITAGAWDGELLDMLEG
                     LAVPERQVLAWLQPTRLEYFRPNAFPVFNLLVDEGRFYGFPVHGVPGFKFGMYHHLEE
                     KVDPETVDREAHDYDERLLRDFAERYFPDGCGPTMDLQTCMFTNTPDNHFIIDMHPEY
                     DQVSFASPCSGHGYKFASVIGEIMADLADTGITRHDVTLFRLDRLTRPFRGLPGRAHP
                     AMGTTDREAHQQNGRPRSFPRVPEIRPFW"
     gene            6589..7362
                     /locus_tag="VNT12_10235"
     CDS             6589..7362
                     /locus_tag="VNT12_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWI46538.1"
                     /translation="METTKRRNNFTQSVDRAVTVMELLSRRGWSGVTEVANELGINKS
                     TAYRLLNTLRDRGLVEQDAATEKYRLGFGLVMLASSVTADLDVARCSRPICDWLAERT
                     RETVTVAVLEGDDAVILYQSNSRSSALSVDWSGMHTPLHATAAGKIFLHYMPGDQQRR
                     ILKRSLQRYTENTIVDPKALREELQRDHGRDYWYTIEELEVGLNAIAAPVRCADGVVV
                     AAVSVSGPAFRLPPDTFQETGALCVRAAEEISRCLGFQG"
     gene            complement(7398..8237)
                     /locus_tag="VNT12_10240"
     CDS             complement(7398..8237)
                     /locus_tag="VNT12_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI46539.1"
                     /translation="MVASGAAEWKLGEFFEYRGDRVAYDVIGEGSPIVLVHGTPFSSY
                     VWRRIAPALAENYRVHVFDLLGYGASDKREGQDVSLYAQGKLLARLLEQWKLESPMIV
                     GHDFGGAITLRAHLLEGCDFERIVLMNAVSVAPWGSPFYRLVQDYPGVFGQIPGYMHR
                     AMMAAYVRDATYRLMTDEETLPYIEPWLGEVGQAALYRQIAQNDQRYTDEVEPLYREI
                     ERPVLVLWGEEDRWLPLEIGEKLHSSIPGSQLRTIPECAHLAQEDATEEVLGHLIEFF
                     SRS"
BASE COUNT         1861 a         2710 c         2806 g         1622 t
ORIGIN      
        1 gcaagctggt cggcgtcgag ctagggggta gcaacctcgg gtcttacaac gacggctcca
       61 tgatcgacta cttcccggtc tataagaacg gcgagagaat cggcaaggtc acctccgcct
      121 gttactcgcc gcgactagac aagagtatcg gttttgcgat ggtgcccgtc gagtacagcg
      181 agataggcat cgagctcgaa atcgagcgtc cggaagaaac ggtcccggcc gtcgtggccg
      241 accgggtctt ctttaagccc gagcatgccg aacaggaatt gagcgccacg agttccaata
      301 agggggccta gcgccggagg aagaagttgc cgcgggcacg gcgtccatag acgcgcccgc
      361 ggcgaccagc gaagagggca tccgagcctc gaagccggcg gaaagatcgt gatcatgaac
      421 aagactgata aggaacagga ggtaagggac gcgctggccg aggccctgcg acgccccagg
      481 tacgaggtga taccgctagg gggcgtggag gaagcgatcg tcgagcacgt acccaagagc
      541 atcaagctta cggtgacggc gtcacccaag aaaggcatcg aagagacgtt gcggctcgcc
      601 gagcggttgt cgggacgggg ctacgaagtg gcgccgcata tttccgcgcg cctcgttcgc
      661 gacgaagcgc acctagagga tgtgctgggg cggctgcgta gaaccggcat taaggatgcg
      721 ttcgtcatcg ctggggacgc cgacgagccc gccggcaagt tcgaaggtgc ggctgcgctt
      781 cttggaacga tggccgaaat tgggcacact ctcgaggaga ttggtataag cggctacccg
      841 gagagccatc ccctcataag cgatgagaca accatccagg cgatgtacga taaggtgccc
      901 tacgccacct acatagtcag ccagatctgc ttcgaccccg cgttgaccgc gcattggatc
      961 cgtcacgtcc ggaatcgggg cgtggagctg cctatatata tcggtatccc cggtgtcgtg
     1021 aacacgacga agctcatgcg gatctccggt agtatcggcc tcggcgagtc ggcgcggttt
     1081 ctcaagaaat atggcaaccg gttcttccgg atgctcttgc ccggtcgcta tagacctgac
     1141 cgtctaatca aggatttaac cccctcgctg acagacccgc aatgcaaagt gaaagggttc
     1201 cacatctaca ccttcaacga gatgggcaag accgaggcgt ggcgcgagaa gctggagacc
     1261 atcgaagcgg ctaagaccgc ctaacgaaga ccacgaggag tcccaaatta tgcaaacgtt
     1321 tccttccgat ttggagattg cgcgacaggc gaagctcaaa ccgttgccgg agatcgccga
     1381 cggcatgggc ataggggagc atctcctgga gccctacggc gactcggtag ctaagataaa
     1441 gctcgaagcc atcgaggagt tggaggaccg cccgctagcg aagtacgtgg tcgtgtcggc
     1501 aataacgccg acacccctgg gcgaaggaaa gaccacgacg accgtaggcc tcgggcaagc
     1561 cttcaagcac atcggcaaga gggcgacggt cgccctccgc cagccggcga tggggccaac
     1621 attcggcatt aagggtggcg ccgccggggg tggttacagc caagtcgtcc cgatggagat
     1681 gctcaacctc catctcactg gcgacacgca cgcggtgacc gccgcgcata acctgctcgc
     1741 cgctatgctc gacaaccacg tccatcagaa cgacgatttt ttccacatcg atcgccacag
     1801 cgtcacttgg cgacgggtgc tcgacgtcaa cgaccgttcc ttgaggaaca ttatcaccgg
     1861 catcggctcg cgggcggacg gcgtgccgcg cgaggcaggc ttcgacatta cggcggcctc
     1921 ggaggtcatg gcgatactag cgttggccac gtcgctgcgc gacctgaggg agcgtctcgg
     1981 ccggatcgtg atagggagca ccaccgacgg gaagccggtt accgcggagg ccctcgaggc
     2041 tgcaggcgcc atggccgtga tcttaaaaga ggcgataaag ccgaacctga tgcagaccct
     2101 tgagaacacg ccggttctgg tgcacgccgg cccattcggc aacatcgcaa ccgggaactc
     2161 ctccatagta gcggaccgaa tcgggatcaa gggaggagac ttcctcataa ctgaggctgg
     2221 cttcggtgcg gacatgggcg ccgagcggtt tttcaacatc aagtgccgct actcggggct
     2281 gaaggccgac gccgccgtcc tcgtcgccac cgtgcgcgcg cttaaggcgc actcgggcag
     2341 gttcaagatc gtcgccggga ggcccttgcc gccggaactc ttggaggaaa accccgacga
     2401 cgtcttcgcc ggggcggaca atctccgcaa gcagatcgaa aacgtccgac tacacggcgt
     2461 ctcgcccgtg gtggcgataa acgccttccc gaccgaccac gcttccgagc acgcggccat
     2521 ccgcgagatc gccgaaggca tgggcgcgcg agtcgccgtc tgcaaccact tcgccgaggg
     2581 tggcgcgggt gccgtggagc tcgccgacgc cgtggcagag gccacagagg agccgacgga
     2641 gttccggttt ctctaccccg aggaggcgag cctcagggag aagatagaga ccatcgcgac
     2701 gaaggtgtac ggggcagacg gcgtggccta cgacatgaag gcctctcagc agctcgactc
     2761 ctacgagaac aacggtttcg ggagattgcc ggtgtgcatg gcgaagacgc acctttctat
     2821 ctcctcggac ccgacgctac tgggggcacc gaagggctgg acgctgccgg tgcgcgaggt
     2881 gcgtgcctcg gtgggggcag gctttatcta ccccctttgc ggggagatgc gtacgatgcc
     2941 gggcctcagt gccaccccgg ccgccacgcg catagatcta gacgacaacg gtgaagtcgt
     3001 aggactctca tgacagatgc cgtacccaac tacgcggagc tgccgctggg ccgcttcgtg
     3061 gacatggtgg cttcccgcga gccggcgccc ggcggaggcg cgtcggccgc cgtggccatt
     3121 gctctcgccg ccgctctcac ggcaatggct gcccggtttt ccgcagataa tctcgccgac
     3181 gccgagacga ttgccgatag ggcggaagaa ctgcgaaaca aggtcatgcc cctcgcgcag
     3241 gccgacgcgg ctgcttacgg gcgcgtgctt gacgcctacc gtaccccgcg cgacgacgac
     3301 gagaagcgac gccgccggat ccgggaagcg ctgtcggagg ccgcggacgt gccactgtcc
     3361 atcgccgagg tcggggtgga ggtggtcggt aacgcggcgc ggctggtcga agaggggaac
     3421 ccgaacctgc gcggcgacgc catgaccgcc gccgccctgg ccaaggctgg cgtacgagca
     3481 gcagccacgc ttgtcgagat caacgtctcc gcaggaggtg ccgacgatga caggttgtct
     3541 cgcgttgacc agcttcttgc cacgatggcc gctgcgcagg aggttgtcga aggcgccgaa
     3601 gcaagaagct ggggctaggc atgggcgcca gcgtcataga cagccgtggc ctggccgccg
     3661 agctcaagga gggctggcgg cggaggtggc tgggcaaagg gacaccgggg tcgtgcccgg
     3721 gctcgcgacc gtgctggtcg gctcgcccgc cgaggcagct ctgcgctcgc tggagccggc
     3781 gcacgcaggt gctgtcatag gaacttgtac aaaatctgtg gaatctgtgg gggaaaggat
     3841 cattcgatga ccgagagaag aacaccgcta tatgacttcc atttccgttc tgcgaggaac
     3901 atagtcaaag gtggcggaga cttcatgttc ccgacctcct acacctcgcc ggtggaggag
     3961 cacctgaacg tgcgcaggaa tgtagggatg caggacctat caacaatggg ggaggtggac
     4021 atcaaggggc caggctccga gaggttggtg aggcgcctgc tcgtcaacga ggtcaacgac
     4081 atggagccgg ggcaactgag gtactccacg atgtgcaacg aggagggcgg aattgtagac
     4141 gatgtcaccg tctacaagtt cggcgacgag cactttatga tcgtcacgag ctccgggcca
     4201 cggctgaaga cgtatcgctg gatctccaag cacgctgagg gcgccagcgc ctacgctacc
     4261 gacgtgacag ctgctctcgc tctgccggtg gtgcaggggc cgcgctcccg gacgttcctc
     4321 aagaccctgg tcgagggtgt ggatctcgat gctctacgct ttttccgctt cgctcagtgc
     4381 cgcatcggcg aggtggagct cattatctcc cgctccggat acaccgggga gctcggttac
     4441 gagttgtaca ctccggcgga tcaggccggc cctctctggg aatacctact ggagaaaggt
     4501 cgcgagtttg acctcaagcc ctacggcgtc gaggcgatgc aatctctacg cattgagaaa
     4561 agcctgcccc tttacggccc tgacataaat gaagaggata cccccttcca cgtggggctg
     4621 gctcgttgga ttcgtctcga gaaacgtgat cttatcgggc gtgaggcctt actgcggatt
     4681 caagagagag gtctcgatag gcgttgggtc ggtctcacct tagagagcga ggtgccggcc
     4741 gctgccggag cgaaagtcta cagcgttggc gacgtggcga ccttccgcga ggtcatagag
     4801 actggtgccg aggccggtga gtacgaggac gcggtcatgc ccggggaccg gcagatcgga
     4861 cgcgtcacct ccagcgccat gggacacagc gtcggcaaga tgctcgcgat ggcctacgtg
     4921 gagaccgccc actcctggcc gggcaacaac ctgatcgtcg aggtcaacgg tcgccccatc
     4981 cgcgctaagg tcgcccaaac cccatacttc gacccggaga acgcccgcat acggtccgag
     5041 cccgcggagg acgggcggcg ttccgagccc gcgcctcgcc tggtggctac gaccgactct
     5101 ggctcgaacg tcccggcgag gcaatatacg aacgggggcc ctggctcgtg agcgtcacct
     5161 actcaggccg ttacgatgct atcgtcgtag gtgtcggggg gatgggtagc gccacctcct
     5221 actacctcgc tcgtcgcggc aagcgtgtgc tcgggctcga gcggttcggc atcccgcact
     5281 ccatgggctc ctcgcacggc cactcccgta tcatccggtt ggcgtattac gagcacacct
     5341 catacgtgtt gctcctcaag cgtgcctacg agctgtggcg ggagatccag cgcatagcaa
     5401 gcgagcggct cctgcacatc accggctcga tagacgctgg ccctgaggac tcctgggtct
     5461 tcaagggctc gtgggagtcc tgtaggctcc acgacctgcc gcatgaggtt ctcaccggcg
     5521 ccgaactgcg ccgtcgctac cccggctatc acctacccgc agaccacctc gcgctcgtac
     5581 aacccgaggg tggcttcctc acgcctgaga agtgcatcgt ctcctacgtg atggccgcac
     5641 aggctttggg ggccgagatc cacgcccacg aacgggtgtt tgagtggcgg ccgttagaag
     5701 gcggggtgcg tgttcgcacc gaccggggcg tctacgaagc ggacaagctt gtcattaccg
     5761 ccggtgcttg ggacggcgag ctgctcgata tgctcgaagg gcttgcggtg cccgagcggc
     5821 aggtcctggc ctggttgcag ccgacccgtc tcgagtactt ccggcccaat gccttccccg
     5881 tcttcaatct gctggtggac gaggggcgct tctacggctt ccccgtgcac ggtgttccgg
     5941 gcttcaagtt tggtatgtac caccacctgg aggagaaagt cgatcccgag actgtcgacc
     6001 gcgaggcgca cgactacgac gagcggctct tgcgagactt cgcggagcgt tacttccctg
     6061 acggctgcgg cccgacgatg gatctgcaga cctgcatgtt caccaacact ccggataacc
     6121 acttcatcat agatatgcac cccgagtacg atcaggtttc cttcgcctcg ccctgctccg
     6181 ggcacggtta caagttcgcc agcgtgatag gcgagatcat ggctgatcta gcagacaccg
     6241 gtatcacgcg ccacgacgtg accctatttc gcctcgaccg actgacacgt cctttccgag
     6301 ggctccctgg cagagcgcat ccggcgatgg ggacaaccga ccgcgaggcc caccagcaaa
     6361 acggcagacc tcgctccttc ccacgggtgc ccgagatcag accgttctgg tagccttctg
     6421 gtggccagtg cagcgtggca attgtccccg cgtcgcaaag aactgggtcc ctcatgtcga
     6481 cgacaacaca cctctgacgg tggtagttgc gaagtgatga aaacgagtga ccggcaccga
     6541 gaatatcagt atcaagccag aacatagtgt taggtgtaga ctacacatat ggaaaccaca
     6601 aaacgtagga acaatttcac gcagtcggta gaccgcgccg tcaccgtgat ggagcttctt
     6661 tcgcgccggg ggtggtccgg cgttacagag gtggctaacg aattggggat caacaagtcc
     6721 accgcctacc gactgctcaa tacgctgagg gatcggggat tggtcgagca ggacgccgcg
     6781 accgagaagt accggctggg tttcgggctg gtcatgctcg caagctcggt gacagcggac
     6841 ctggacgtcg cgcgctgctc ccggccgatc tgtgattggc tggccgaacg gacgcgggag
     6901 acggttaccg tcgccgtact ggagggggac gacgctgtta tcctctacca gagcaattcg
     6961 agatcttcgg ccttgagtgt ggactggtcg ggtatgcaca ctccgctgca tgccactgcg
     7021 gcgggtaaga tcttcttgca ctacatgcct ggagaccagc agcgtcgcat tctcaagagg
     7081 tcgctgcaac gctacaccga gaatacgatc gtagatccca aagccctccg agaggagcta
     7141 cagcgcgacc acggtagaga ttactggtac accattgaag agctggaggt tgggctcaac
     7201 gcgatagccg caccggtccg ctgcgccgac ggtgtagtgg tagccgcggt gagcgtctcg
     7261 ggaccggcat tccggttgcc tcccgacact tttcaggaga ccggcgcact atgcgtgcgg
     7321 gctgccgagg agatctctcg ctgtttgggt tttcagggct gaaccagcct accggatgac
     7381 cacaggcagg gtctctgcta actacgagag aaaaactcga tgaggtggcc caggacctct
     7441 tcggtagcgt cttcttgagc caggtgggca cactcgggaa tcgtcctgag ttgtgaaccc
     7501 ggaatcgagg agtgcagttt ctcccctatc tctagaggca gccagcggtc ctcctctccc
     7561 cacaggacga gcacggggcg ctcgatttct ctatagagcg gctcgacttc gtcggtgtag
     7621 cgctggtcgt tctgcgctat ctggcggtag agggcagcct gtcccacttc gccaagccag
     7681 ggctctatat agggaagcgt ttcttcgtcg gtcatcagcc ggtaggtggc gtccctaacg
     7741 taggcggcca tcatcgcccg gtgcatgtag ccggggattt ggccgaatac gcccgggtag
     7801 tcctgcacca gccggtagaa cggagagccc cagggggcaa cggagactgc attcatcagg
     7861 acgattcgct cgaagtcgca tccttccagg agatgcgccc tcaaggtgat cgcgccgccg
     7921 aagtcgtgac cgacgatcat ggggctttcc aacttccact gctccagcag cctagccagc
     7981 aacttgccct gagcgtagag ggagacgtcc tgaccctctc gcttatctga agctccatac
     8041 cccagcaggt cgaaaacatg caccctgtag ttctccgcca gcgcaggagc gatcctacgc
     8101 cagacgtaag aggagaaggg cgtaccgtgc accaggacga taggcgatcc ctctccgatc
     8161 acgtcgtagg cgacacgatc acccctgtac tcgaaaaact cccccagctt ccactccgcc
     8221 gctccacttg ccaccattca cctccaaggc tccgctcaag ggtatgctgg tgatcctcct
     8281 catgccagca tcgtcgggtg ctgatcttat cctttccaac gaaaatacaa gatgctgcca
     8341 ttctaaagtg acacaaaagt tgaccctagt ttgaccctaa cccggacgca atgcactgca
     8401 atagtgagca atcgaggaaa cagagaaccg cttagaaatg cggaattagc aagcccgtgc
     8461 aacgctaggc aaccactgac cgcacactcc taatgagcag gtcagcgatc cgagggtgtt
     8521 cgaatggatg tactgcaacc gtactgcaac cgggctagta caggctagta cgcagtggac
     8581 aaagtgagtc tgccagatca tcgaaaactg cataaacaag ccgaaaagcg gacgcaccag
     8641 tacgggctag aacgcgtgca agcaaactcg tagctggagc aggtcagcgg ttcgagtccg
     8701 ctcgtcggct cttaatttta ttgcgatttg caggaaaaac gtaagtcctg cagaactcct
     8761 tccagcctaa gttacagcaa ccgtactgca acccgagctt ggctaaaaac tacattaagg
     8821 ccacaagaat acatcgctta ctctttaagt agatgaatca taggaagtgc gtgcttctgg
     8881 acttggactt cccctttacg gcgttctacg aagtgagggc gcttccttgg tgatgtggag
     8941 cacaaactgc tctggagcac atacactccc ctaagacacg agtatgctgc atacgttac
//
LOCUS       DATMJA010000429         7130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_295023, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000429 DATMJA010000000
VERSION     DATMJA010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295023"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..326)
                     /locus_tag="VNT12_10245"
     CDS             complement(129..326)
                     /locus_tag="VNT12_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46540.1"
                     /translation="MEHRKSEAEVALELMRLVLEHAGSRRLSPTRTLALYHLCKEAVA
                     YSPEDRVSADYLEENLKKLLQ"
     gene            479..1147
                     /locus_tag="VNT12_10250"
     CDS             479..1147
                     /locus_tag="VNT12_10250"
                     /inference="COORDINATES: protein motif:HMM:NF021501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HWI46541.1"
                     /translation="MGESSRLYVVCPVCGHVFRSTYASTYITVGREADLCPKIPGRPS
                     DGARLIRSAVTMCPVCSFAAGEAFDDLDLTFDERYGIEERLKEDGLLKVFRKGQPPWL
                     GFHAAEVCGKERSLRSRELGDLCLRASWVCRKEKERPFESTFQLRALRHFMRSLQEDD
                     LIGRELSVTTYLVGELNRRLGNHREALNWYVNAGRTTEGDPRVAWLDRLIDRQSKLAR
                     EQAA"
     gene            complement(1214..2050)
                     /locus_tag="VNT12_10255"
     CDS             complement(1214..2050)
                     /locus_tag="VNT12_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWI46542.1"
                     /translation="MGMKVYRAGRQSPDSKSQGRVLRSLLLILLVILALVLVYLILPF
                     GGQRVVLLGSDARAGEASRSDTIVVTKAGGGMLAVPRDTLVDIPGVGEDKINAAFASG
                     GPELTVETLENLTGVRMNDYVIVHFGGVEEIVDALGGITLEVDKPIKVGIDGRQVYIP
                     AGTHTLNGMQALAYVRYRGGPTADIGRIGRQQKFLRALAHESTSPAKLPRLPATATAT
                     WRNIDTNMNPLEAARFAVRTRFSGIENAELYPGTPQYIGGISYWVPDKEAGDKVVSET
                     IE"
     gene            2201..3004
                     /locus_tag="VNT12_10260"
     CDS             2201..3004
                     /locus_tag="VNT12_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46543.1"
                     /translation="MEKLETASFEIEEERVAWEGLASSCTAPIRRFAAFTLVGFTSFI
                     VITTAVILFYTVQGVAVPWTAFDVTMLLGVAVATGGYLIWLIRSLRSYAAFSAVLSRG
                     GLDPHRPTAWGLRAYSDEQLLALRSRYERLHDGKLRERLGRIFGFHGDDEFSLGPLSA
                     LPGTFEMGALRVEWETQLILHSGEPMPEISWWTEGRHNLLPRRPDESRKLVYALRYTG
                     DSVRELKRRYGYSTARWHATVPEGKLFDAVRDLESSHRIQLALSRRRPG"
     gene            3146..4240
                     /locus_tag="VNT12_10265"
     CDS             3146..4240
                     /locus_tag="VNT12_10265"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWI46544.1"
                     /translation="MESGFGATAPFTVGVEEEFQLVDPASRGLTPAVDEVLAAGEGSR
                     RITSELSRSCVEMLSPVFATAADLARELPGLRREVRDLARSRGVEIVAAGAHPFSEPT
                     EQPLTGGVHNRRVEEEMGWVARTQAIYGLHVHVAVPDGDAAILAVNALARHVPLLVAL
                     SANSPFWRGFDTRLASTRIKIFEMFPRSGLPPTFRNWEDFEDHIEALVASGSIPDYSW
                     CWWDARPHQKFGTVELRAPDVQTDASYTVALAAFVQCLVARAGMDERPPEDALYNAEN
                     KWRAVRYGMDADFYDFAAGRVVPARSMARGLVEELRPVARDLGCEEELGGVLEIVELG
                     TGAELQRAALGRSGSLEGVMDYLIEGTAPD"
     gene            4325..5974
                     /locus_tag="VNT12_10270"
     CDS             4325..5974
                     /locus_tag="VNT12_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWI46545.1"
                     /translation="MASELTLGPLLRYAGESDATVWVETDAACEVGVLGHTARTFKIE
                     GHHYALVRLTDLEPGKTYKYEVFLDGAKVWPDDEHDFPPSVIRTIAPDGRLNLSYGSC
                     RVSVPHESPFTLASDAHKEGYERDALQALALRMMREPHERWPDALLLLGDQIYADEVS
                     EGALDFIRSRRDTNEPPGEQVADFEEYTRLYWDAWRNPVIRWLLSTVPTTMIFDDHDV
                     NDDWNISKTWVRQMRAKPWWEERIIGAFMSYWVYQHLGNLSPRELEEDGLFEKVQEAE
                     NPTRILREFAYKADREIAGTRWSYHRDFGKVRLIMMDSRAGRVLKEGHRSMLDEEEWA
                     WIVEKATGDFDHLLFGTSLPLLLGPGLHHLEAASEAVCAGAWGRPAARIGESVRQLVD
                     LEHWPAFQRSFTDLAHLLRSVAAGERSNGDPPASVTVISGDVHHGYLAKMDFGDGVET
                     PVYQSASSPLRNPLGLPERLAMRFGWIERGRHVGEILSRLASVEEPDIRWHLVHEAPW
                     FHNHISSLEIRGRKALLKVEKTTPENNDEPHLYRILEHRLA"
     gene            6106..6504
                     /locus_tag="VNT12_10275"
     CDS             6106..6504
                     /locus_tag="VNT12_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI46546.1"
                     /translation="MKVVTVKDMRTGPGPEDWFTGTVWRDAAPVGAPPDVAVNRVLFE
                     PGARTHWHTHPEGQILYIITGTCRVGKEGEAPVEVEAGGVVYFAPDEKHWHGATPGTY
                     MVHIAINIATTTDGGTYWMEPVVDEEYAGE"
     gene            complement(6513..>7130)
                     /gene="nrfD"
                     /locus_tag="VNT12_10280"
     CDS             complement(6513..>7130)
                     /gene="nrfD"
                     /locus_tag="VNT12_10280"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWI46547.1"
                     /translation="QAAEDGLLGDDNLLVHLVRLIPARALSILALPVGINVGSNTGNL
                     ISATSVPLWARNWLFMGPTFLASGLSTALSWLSLVLHLTRSGEEKTLLTLHRAEKATI
                     VIEAGLIAASLVRMSKWSKPLFSRAVAPLFVGGTLLGGIAAPMALLFGKASRSRSIIA
                     SVLALAGGLAFRFAIIKGGRISADDPEAYFTFASGESAPQPENNI"
BASE COUNT         1300 a         2256 c         2282 g         1292 t
ORIGIN      
        1 agacctcctt tatccgtacc ggtgagagta tgctatgaca ctcctcgccg cacaagtagc
       61 cacgaactcc gcaacacctg acgaaaggga cgaattggtg ggctgacggc tataattttg
      121 ggtagatgct attggagcaa cttcttcagg ttttcttcta ggtagtctgc gcttacgcgg
      181 tcttccgggg aataggcgac ggcctccttg cagaggtggt agagcgccag agtacgggtc
      241 ggggagagtc gcctacttcc tgcgtgttcc aggacgagtc gcatcagctc cagggcgacc
      301 tcggcctcgc tcttgcgatg ctccatacgg gtgcctccct ccgggccggt ctgtgcaggg
      361 aattataccg gcgaagcgag cggctatggt agaattgggg acgctttggt cccagatcca
      421 ggccccaaga gggactcaga aacgcggccc ggcggcccgc gagaggttag ggatgcccgt
      481 gggtgagtcg agcagacttt acgtcgtctg tcccgtctgc ggccacgtgt tcaggtccac
      541 ctacgcgagc acttacatta ccgtcggcag ggaggccgac ctctgtccca agattcctgg
      601 ccgcccctcg gacggggcgc gtttgatccg gagcgccgtg accatgtgcc cggtgtgctc
      661 gttcgcggca ggcgaggcgt tcgacgacct cgacctgact ttcgacgaac gatacggtat
      721 agaggagcgt ctgaaggagg acggcctgct gaaggtcttc cgcaagggcc aacccccctg
      781 gctaggcttc cacgcggccg aggtctgcgg caaggaaagg agcctgaggt cccgtgagct
      841 gggcgacctg tgcctgcgcg cctcctgggt ctgccgcaaa gagaaggagc gtcccttcga
      901 gagcaccttc cagctgaggg ccctgcgcca ctttatgcgc tccctgcagg aggacgacct
      961 catcgggcgc gagctctccg tcaccacata cctcgtaggg gagctgaacc gccgtctcgg
     1021 caaccatcgc gaagccctca actggtacgt aaacgcggga cgcacgaccg aaggcgaccc
     1081 ccgcgtagcc tggctcgacc gtctcattga caggcaaagc aagctcgcca gagagcaggc
     1141 ggcgtgatag ctggtcagca cgctacggct tgcgcctccg cttctcagca cgaaacctga
     1201 tacctgaagc ttgttattcg atcgtctctg atacgacctt atccccggcc tctttgtccg
     1261 ggacccagta ggagatgccg cctatgtact gtggggtgcc tgggtagagc tcggcgttct
     1321 cgatgcctga gaagcgggtg cggacggcga agcgggcggc ttcgagaggg ttcatgttcg
     1381 tgtcgatgtt gcgccacgtc gccgttgctg tggcggggag acgcggcagt ttggcgggcg
     1441 aggtggattc gtgcgccagc gcccgcagga acttctgctg gcggcctatc ctcccgatat
     1501 ccgccgtcgg gccgcccctg tagcggacgt aggcgagcgc ctgcatcccg ttcaaggtat
     1561 gggtgcctgc cggtatgtag acctgacgtc catcgatacc gacctttata ggtttgtcta
     1621 cttcgagtgt gatcccacct agagcgtcga ctatctcctc aaccccgccg aagtgtacga
     1681 tgacgtagtc gttcatccgg acgccggtca ggttctcgag cgtctcgacg gtcaactccg
     1741 ggcctccgct ggcgaatgcg gcgttgatct tatcctcccc gacaccgggg atgtccacga
     1801 gcgtgtctcg cggcacggcg agcataccgc cccccgcctt ggtgaccacg atagtgtcag
     1861 agcgcgaggc ctccccggcc cgcgcgtcgc tccccagcaa gaccacccgc tgcccgccga
     1921 acggtaagat caggtagacg agcacgaggg cgagaattac tagcaggatc aggagtaagg
     1981 atctgagtac tctaccctgg ctcttactat ccggactctg acgtcccgcc ctgtagactt
     2041 tcatgcccat cactatattg tagtgaacga tggtgaggtt ggcgtcgcag cgatgacgcg
     2101 cggcggcgct tcgccgtgta agcgctacaa ggcgcagttc tcctatgggc gtgatctcta
     2161 acccattttc gactggcggg atacggttat actggaggac gtggagaagc tggagacagc
     2221 gtcgttcgag atcgaagaag agcgggtggc gtgggaaggc ctggcctcgt cttgtactgc
     2281 gcccatccga aggtttgcgg cttttactct ggtcggcttc acctccttta tcgtcattac
     2341 gaccgccgtc atcctgttct atacggtgca gggcgtggca gtgccttgga ctgcgttcga
     2401 cgtgacgatg ctcctcggcg tcgccgtcgc caccggaggc tatctgatct ggctcatcag
     2461 gagcctgagg tcgtacgcag ccttctcggc agtactcagc agaggcggcc tggatccgca
     2521 tcgcccgacc gcatggggac ttagggcata ctcagacgag cagttgctcg ccttgcgctc
     2581 ccgctacgag cggctgcacg atgggaagct cagggaacgc ctcggacgca tcttcgggtt
     2641 ccacggagac gacgaattct cgctgggacc gctgagtgcg ctcccaggta cgttcgagat
     2701 gggtgccttg agagttgagt gggagacgca gttgatcttg cactcaggcg aaccgatgcc
     2761 tgagatctcc tggtggaccg aggggcgtca caacctccta ccgcgcaggc ccgacgagtc
     2821 ccgcaaactc gtctacgccc tccgctacac gggcgactcc gtgcgcgagc taaagcgccg
     2881 ctacggctac agtacagcgc gctggcacgc cactgtcccc gaaggcaagc tcttcgacgc
     2941 cgtccgcgac ctcgaaagct cccaccgcat ccaactagcc ctcagtcgta gacgccccgg
     3001 ttagagcgtg taggggaagg gccttcctcc tacacacgct ggtcagcatt tcagcacgct
     3061 ccggcctccg cttgtcagca cttcagcaag gtctcgtgca cgcgcgagct gatatgctga
     3121 aatgctgaag tgctagagta ggcggatgga gagtggattc ggcgcgacgg cgccctttac
     3181 ggtcggggtc gaggaggagt ttcagctggt cgacccggcc tcgcgcgggc tgaccccggc
     3241 ggtggacgag gtgctcgccg cgggggaggg ctcgcggcgc atcacctcgg agctctcgcg
     3301 gtcttgtgta gagatgctct cccctgtctt cgcgacggcc gccgacctcg cgcgcgagtt
     3361 gcctgggttg cgccgggagg tgcgtgacct cgcgcgcagc cgcggggtcg agatcgtggc
     3421 ggcgggcgcc catcctttct ccgagccgac agagcagcca ctcacgggcg gggttcacaa
     3481 ccggcgcgtg gaggaggaga tgggctgggt agcgaggaca caggctatct acggactgca
     3541 cgtccacgtt gcggtccctg acggtgacgc tgcgatcctg gccgtaaacg cgctcgccag
     3601 gcacgtgccg ctcctcgtgg cgctctcggc caactcgcct ttctggcgtg ggttcgacac
     3661 caggctcgcc tcgacccgga tcaagatctt cgagatgttc ccccgatccg gcctcccgcc
     3721 aaccttccgg aactgggagg acttcgagga ccacatcgag gccctcgtcg cctcggggag
     3781 catccctgac tactcctggt gctggtggga tgcgcgtcca caccagaagt tcggcaccgt
     3841 ggagttgcgc gcgccggacg tacagacaga cgcgtcctac acggtggccc tcgccgcgtt
     3901 cgtccagtgc ctcgtagcta gagccggtat ggacgagcgg cctccggagg acgcactgta
     3961 caacgcggag aacaagtggc gcgccgtacg ttacgggatg gacgccgatt tctacgactt
     4021 cgcagcaggc agggtggtac cggcccgttc gatggcccgt ggactggtcg aggagctgcg
     4081 gcctgtcgcc agggatctcg gctgcgagga agagctgggt ggtgtgctcg agatcgtgga
     4141 actcggcaca ggggcggagt tgcagcgggc cgccctgggg aggagcggct cgctagaagg
     4201 cgtgatggac tatctgatcg aaggtaccgc gccagactag ccgtgcggta acgtcttccc
     4261 ccatccccat aaccaggatg gattgttctg gcacgagaga gccggctgcg ggtagaattc
     4321 ccgcatggct tctgaactga ccctcggtcc gttgctgcgc tacgcgggag agagcgacgc
     4381 gaccgtctgg gtcgagacag acgccgcctg tgaggtcggg gtcctcggcc acacggcacg
     4441 caccttcaag attgagggcc accattatgc cctggtcagg ctcacggacc tcgaaccggg
     4501 gaagacctac aagtacgagg tcttcctcga cggcgcgaag gtctggcccg atgacgaaca
     4561 cgacttcccg ccttctgtta tcaggacgat cgcgcccgac ggaagattga acctctccta
     4621 cggttcttgc cgcgtctccg tgccgcacga atctccgttc acactcgcct cggacgctca
     4681 taaggaaggc tacgagcggg atgccctgca ggctctggcc ctgcgcatga tgcgcgagcc
     4741 ccacgagaga tggcccgacg cgctgctact attgggggat cagatctacg ccgacgaggt
     4801 ctccgagggc gccctcgatt ttatccgctc gcggcgcgac acgaacgagc cgcctggcga
     4861 gcaggtcgcc gatttcgagg agtacacgcg cctgtactgg gatgcctgga ggaaccccgt
     4921 catcaggtgg ctcctctcga ccgtacctac gacgatgatc ttcgacgacc acgacgtcaa
     4981 cgacgactgg aacatatcga agacgtgggt ccgccagatg cgcgccaagc cgtggtggga
     5041 ggagcgcatc atcggtgctt tcatgtccta ctgggtctac cagcacctgg gcaacctctc
     5101 cccgcgagag ctggaggagg atggactctt cgagaaggtg caggaggccg agaaccccac
     5161 ccgcatcctg cgcgagtttg cctacaaggc ggaccgcgag atcgccggta cgcgctggag
     5221 ctaccaccgc gacttcggta aggtccgtct catcatgatg gactcgcggg caggtcgggt
     5281 actgaaggaa ggccaccgct ccatgctcga cgaggaggag tgggcctgga tcgtagagaa
     5341 agccacgggc gacttcgacc acctcctctt cggcacctcg ctccctctcc tccttggccc
     5401 cggtcttcac cacctggaag cggccagcga ggccgtctgt gccggcgcct ggggccgccc
     5461 cgcggccagg atcggggagt cggtgcgcca gctcgtcgac ctcgagcact ggcccgcctt
     5521 ccagagatcc ttcaccgacc tcgcgcacct gctacgctcc gtcgccgcgg gcgaacgctc
     5581 gaacggcgac cctcccgcct ccgtaacggt catttcaggc gacgtgcacc acggctacct
     5641 ggcgaagatg gacttcggcg acggcgtcga aacccccgtc taccagtcgg caagctcccc
     5701 cttgcggaac ccgctaggcc tccccgaacg cctcgccatg cgcttcggct ggatcgaacg
     5761 gggcaggcac gtcggcgaaa tcctctctcg cctggccagt gtcgaggaac cggacatccg
     5821 ctggcatctc gtacacgaag cgccctggtt ccacaaccac atctccagcc tcgagatccg
     5881 gggccgcaaa gccctgctga aagtggagaa gaccaccccg gagaacaacg acgaaccgca
     5941 cctgtacagg atcctcgaac accgtctggc atgaatagca tttcagccag tcagcacgcc
     6001 gccttgcagg cggctcgtca gcctgtcagc aagaaaccca gcctgacaag ctgaaatgct
     6061 gacttgctga caggctgaga tgcttccgaa aagagggagg aacccatgaa agttgtcacg
     6121 gtgaaagaca tgcggaccgg gccggggccc gaggactggt ttaccggcac ggtatggaga
     6181 gatgctgctc ccgtcggcgc cccacccgac gtggccgtca accgcgttct gttcgagcct
     6241 ggcgcccgca cacactggca cacccatccc gaaggccaga tcctctacat catcaccggc
     6301 acctgccgcg tcggcaaaga aggcgaagcg cctgtcgagg tggaggccgg aggcgtcgtt
     6361 tacttcgcgc cggacgaaaa gcactggcac ggtgccaccc ccggaaccta catggtccac
     6421 atcgccatca acatcgccac cacgaccgac ggtggcacat actggatgga gcccgtcgtg
     6481 gacgaggagt atgcaggcga gtaactcgct cgctagatat tgttctcggg ttgcggagca
     6541 ctctcgccgc tggcgaaggt gaagtaggcc tcagggtcat cggcgctgat gcggcctccc
     6601 tttattatgg cgaagcggaa ggcgagaccg ccagcgagcg cgagcacgga agcgattatg
     6661 ctcctggagc ggcttgcttt gccgaagagc agggccatcg gcgcagctat gccgccgagg
     6721 agggtgccgc cgacgaagag cggggcgacc gctctggaga agagcggttt gctccacttg
     6781 ctcatcctta ccagggaggc ggctatcagc cccgcctcta tgacgatggt cgccttctcg
     6841 gcgcggtgga gggtaagcag ggtcttctct tcgcccgaac gtgtaaggtg gagcacgagc
     6901 gagagccacg agagcgccgt cgagagtccc gaagccagga acgtcggtcc catgaacagc
     6961 cagttgcgcg cccacagcgg gacggaggtg gcggagatca ggttcccagt gttggagccg
     7021 acgttgatcc caacgggcag tgccaggata gagagcgcgc gcgcaggaat gagcctcacg
     7081 aggtgcacca gcaggttatc gtctccgagc agaccgtctt cggcggcctg
//
LOCUS       DATMJA010000430         4539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_236037, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000430 DATMJA010000000
VERSION     DATMJA010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236037"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1206
                     /gene="dnaJ"
                     /locus_tag="VNT12_10285"
     CDS             70..1206
                     /gene="dnaJ"
                     /locus_tag="VNT12_10285"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWI46548.1"
                     /translation="MATKRDYYEILGVSKEASDAEIKKSYRRLARSHHPDANPGDHEA
                     EERFKELTEAYEVLSNPEARRAYDTYGHQVPRGAGAAGYPGGGDPFGGFQDIFEAFFG
                     DRSFGGSFFGTSTAQGPSRGSDAEVEVEVSLYEAAFGADREVNVQVVRNCRVCDGVGG
                     TESHVCPTCHGAGSVRTVRESILGQMVTTQACSTCGGGGRVIDVVCDNCRGSGRISEV
                     QARRLRVPVGIESGMRLRVSGGGHAGDPGAPPGDLYVRINVKEDQELMRDGEDLIHRM
                     RISFVDAALGAEVDVPTLEGPKQVRVDPGTQPGATLTLRGDGMPRLRRRGRGDLKVVV
                     DVMVPTRLTAEQRDLLERFEATSGEETYNGGGGSFFDRLRGVFR"
     gene            1272..1931
                     /locus_tag="VNT12_10290"
     CDS             1272..1931
                     /locus_tag="VNT12_10290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWI46549.1"
                     /translation="MLELPPDETNHVHNVLRGRNGDLIEVVDGDGRLFAARLRSGGEA
                     AVVEELEAPGGTGVEISLYQAAPKGRRMDLVVEKATEVGATRIVPLLAERGVVSPREG
                     KVGRWQRVAEAAARQALRLSVPEVAEPVRFEDAASEVRETGVLLHNAPDLQPVEAVVG
                     APVSLFVGPEGGWSEGELLLAEEAGFTFGGLGPYRLRSETAGIIAVARTVAALESRVE
                     R"
     gene            1943..2287
                     /locus_tag="VNT12_10295"
     CDS             1943..2287
                     /locus_tag="VNT12_10295"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HWI46550.1"
                     /translation="MSEADCIFCRIVRGEIDAEVVHDEDDVLAFEDINSKAPVHVLVI
                     PKQHVANLEEIGALPDTVVKRLFEVASAIAEKLGVTEGGYAVMINNGRDAGQEVFHLH
                     LHVMGGRRLGMP"
     gene            2284..2727
                     /locus_tag="VNT12_10300"
     CDS             2284..2727
                     /locus_tag="VNT12_10300"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWI46551.1"
                     /translation="MTIRERIQSDTKAAMKQRDRTRVGALRMLGAALKNAEIEAGRPL
                     TEQEEQTILRRQLKQRDESAEAFRKAGREEKAASELAEAEIVRSYLPEPPSQKELEEI
                     VDMAINETAAQDMKDMGGVMARAMELSEGRAEGRTLSALARTRLQ"
     gene            2921..3760
                     /locus_tag="VNT12_10305"
     CDS             2921..3760
                     /locus_tag="VNT12_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWI46552.1"
                     /translation="MRGNEIVLRGTEGSVERAEAVFDGLVGLAEEGHHPTLETAERLY
                     KMADEGGGREVLGDVILTHRGRRVAPKTRNQKAYTDAIRQHQVVFGIGPAGTGKTYLA
                     VALAVEALKRGEVSRIILTRPAVEAGESLGYLPGDVMAKVDPYFRPLYDALYEMVDPA
                     KFQAHLERGIIEIAPLAFMRGRTLNDAFIILDEAQNTTPQQMKMFLTRLGFGSKMVIT
                     GDITQVDLPKARISGLQDARNTLTGVEGVSFVGLRRDDIVRSDLVMRIVDAYEVAGEG
                     DRA"
     gene            3782..>4539
                     /locus_tag="VNT12_10310"
     CDS             3782..>4539
                     /locus_tag="VNT12_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46553.1"
                     /translation="MKIGKTSLKKQPDRKVDEFHRSPTRFELFRAWIERIPKIRLYIG
                     LTALTWLSLTALIGIGPRPLIERIISQGIIDRRDRTRLESLDLLQQSDPWMALVGVGI
                     VVAAEMWISWYFLERFGERILKTNSAIRLVLAASLTVLFTALARLFIELDFDPYLTPL
                     AGLSIIGTMLLGPRLMFLMVVITSVNVGILSGNNFLLTAALLLGSGFAIYTTVRVDSR
                     QRLLKAGLVIAAVMGVVTFAVGLIGGGDPKDALWL"
BASE COUNT          923 a         1312 c         1534 g          770 t
ORIGIN      
        1 gacgccgcca taacgacggt gcgctcggcc gcgcaggccc tgaccaactg tctggacgcc
       61 aggttctagt tggcaaccaa gcgcgattac tacgagattc tgggcgtgtc caaggaagcg
      121 tcggacgcgg agatcaagaa atcttaccgc cgcctcgccc gctctcacca ccccgacgcg
      181 aacccaggcg accacgaagc cgaagaacgc ttcaaggagc tcaccgaggc ctacgaagtc
      241 ctctccaacc cggaagccag acgggcctac gatacatacg gccaccaggt accgcgcggc
      301 gcaggagcgg caggctaccc cggtgggggg gacccgttcg gaggcttcca ggacatcttc
      361 gaggctttct tcggcgaccg ctctttcggc ggctccttct tcggtacctc caccgcgcag
      421 ggcccgagtc gtggtagtga cgctgaggtc gaagtagaag tctccttgta cgaggccgcg
      481 ttcggggctg accgtgaagt caacgttcag gttgtaagga actgtcgggt ctgcgacggc
      541 gtagggggaa cggagtcaca cgtctgtcct acctgccatg gtgccggctc tgtaaggacg
      601 gtgcgggaga gcatcctcgg gcagatggtt acgacgcagg cgtgctcgac ttgtggcgga
      661 ggtggccgcg tcatcgatgt cgtgtgcgac aactgtcgcg gcagcgggag gatctccgag
      721 gtacaggcca ggcggctgcg ggtgcctgtg ggcatcgaga gcggcatgag gctgcgcgtt
      781 tcaggtgggg gacacgcagg agatccagga gcgccgccag gggacctcta cgtgaggatc
      841 aacgttaaag aagaccagga gctcatgcga gacggggagg acctgatcca ccgcatgagg
      901 atcagcttcg tcgatgcggc gcttggggct gaagtagatg tgcccaccct ggaaggaccg
      961 aaacaggtga gggtggatcc cggcacccag ccaggtgcga cgctgacgct caggggtgat
     1021 gggatgccgc ggttgcggcg gcgtgggcgc ggagacctca aggtagtcgt cgacgtaatg
     1081 gtaccgacac gtctaacggc cgagcagcgc gacttgctcg agaggttcga ggctaccagc
     1141 ggagaagaga cttacaacgg tggtggaggc tccttcttcg accgtctcag gggtgtattc
     1201 aggtaggggg cttcgttacg cgcacgccca taacccgtat cttcgtcgac tccatactgg
     1261 agctaggcgg ggtgctggag ctgccgccgg acgagaccaa ccacgtccac aacgtcctgc
     1321 gtggcaggaa cggggatctt atagaagttg tggacggcga tgggaggctc ttcgctgcca
     1381 gattgcgcag cggaggagag gcggctgtcg tagaggagtt ggaggcgcca ggtgggaccg
     1441 gcgtggagat ctcactctac caggcggcgc ccaagggccg aaggatggac ctcgtcgtcg
     1501 agaaggctac ggaggtcggt gccacgcgta tcgtgccgct gttggccgag cgtggggttg
     1561 tgagcccgcg cgaggggaag gtagggcgct ggcagcgggt ggccgaggcg gcggcacgcc
     1621 aggcgttgag gctcagcgtg cccgaagtgg ccgaacccgt acgattcgag gacgccgcct
     1681 cagaggtgag agagaccgga gtcctcctcc acaatgcccc cgacctccag ccagtcgagg
     1741 ccgtcgtcgg ggctcccgtg agcctctttg taggcccgga gggtggatgg agcgagggcg
     1801 agttgctgct cgctgaggag gctggcttca ctttcggcgg cttgggaccg tacaggctcc
     1861 gcagcgagac cgccggtatc atcgcagtgg cgcgtacggt ggccgcattg gagagcaggg
     1921 tcgagcgtta gaggaggtgg ccgtgagcga ggccgactgc atcttctgta gaatagtgcg
     1981 aggggagata gatgccgagg tggttcacga cgaggacgat gtgctggctt tcgaagacat
     2041 aaatagcaag gcacctgtcc acgtcctcgt catccccaag cagcacgtag cgaacctgga
     2101 ggagatcggt gcattgcccg acaccgtggt caagcggctc ttcgaggtgg caagcgccat
     2161 cgccgagaag ctcggcgtaa ctgagggtgg gtacgccgtc atgatcaaca acggccgcga
     2221 cgcagggcag gaagtctttc acctccacct gcacgtcatg ggcggcagga ggctgggaat
     2281 gccgtgacca taagagagcg catacaaagc gacaccaagg cagcaatgaa gcaacgcgac
     2341 aggacccgcg tcggcgccct gcgtatgctc ggcgccgcgc tcaagaacgc cgagatagag
     2401 gcggggaggc cgctcaccga gcaggaagag cagaccatcc tcaggcgcca gctcaagcag
     2461 cgcgacgagt cagcagaagc gttccgcaag gccggacgag aggagaaagc agcatccgag
     2521 ttggccgaag ccgagatcgt ccgctcctac ctccccgagc cgccctcgca aaaggaactc
     2581 gaagaaatag tcgacatggc catcaatgaa acagcagctc aggacatgaa agacatgggg
     2641 ggggtcatgg cccgtgccat ggagctctcg gaaggccgtg ccgagggccg caccctctcc
     2701 gcactagccc gcaccagact acaatgaagg caacaggttc caggtatgag aaagctgaaa
     2761 tgctgacgag cggaggccga agggaggcca cacggaaacc acaaccggca gccaggtaga
     2821 agtcaagctc gtcataccgc caggcaggac actcgaggtc ttcggggacc gtgactccgt
     2881 cctgaggcgg gttgaggagc tcgtcgagtg cgatgtcgtc gtgcggggca atgagatcgt
     2941 cctgcgaggt accgaggggt ctgtggagag ggccgaggct gtcttcgatg gcctcgtcgg
     3001 gctcgctgag gagggacacc accctaccct ggagaccgcc gagcgcctct acaagatggc
     3061 cgacgaaggc ggcgggcggg aggtgcttgg ggatgtgatc cttacgcatc gcgggcgccg
     3121 cgtcgcaccg aagacccgca accagaaggc gtatacagat gctattcgcc aacatcaggt
     3181 tgtcttcggc atcgggccgg cgggcacggg caagacatac ctggccgtgg ccctggccgt
     3241 cgaggctctc aagcggggag aggtgtctag gatcatactg accaggcccg ctgtcgaggc
     3301 cggcgagtcg ctcgggtacc tgcctggcga cgtgatggcc aaggtagatc cctacttcag
     3361 gccgctctac gacgcgctct acgagatggt ggatccggcg aagttccagg cccacctgga
     3421 gcgcggtata atcgagatag cacccctggc atttatgagg gggaggacgc ttaatgacgc
     3481 atttattatc ctggacgagg cgcaaaatac cacccctcag cagatgaaga tgttcctcac
     3541 gcgcctcggc ttcggctcaa agatggtcat tacaggcgac ataacacagg tggatctccc
     3601 gaaagcccgc ataagcggcc tgcaagacgc caggaacacg cttacgggcg tagaaggcgt
     3661 atcattcgtc gggctgaggc gcgacgacat cgtgcgtagc gacctcgtca tgcgtatagt
     3721 cgatgcctac gaagtggcgg gagagggcga cagagcctag gcctcagggc cgggagcgac
     3781 catgaaaatc gggaagacaa gcctcaagaa acagccggac aggaaggtcg acgagttcca
     3841 ccgctcgccg acacgcttcg agctcttcag ggcctggatc gagaggatcc cgaagatcag
     3901 gctctacatc gggcttaccg ccttgacctg gctctctttg accgcgctca taggtatagg
     3961 accgaggccc ctgatcgaac gtatcatctc ccagggaatc atagaccgga gagatcggac
     4021 gcggctcgag tcgctcgacc tgctccagca gtcggacccg tggatggctc tggtcggggt
     4081 cggcatcgtc gtcgctgccg agatgtggat ctcctggtac ttcctggagc gcttcgggga
     4141 acgcatactc aagacgaact cggcgataag gctcgttctg gctgcctcgc tcacggtact
     4201 tttcaccgcg ctcgccaggc tctttatcga gctcgatttc gatccctatc tcacgccact
     4261 cgcaggcctc tccatcatcg gaaccatgct cctcgggccg aggctcatgt tccttatggt
     4321 ggtgatcacc agcgtcaacg taggtatcct cagtggcaat aacttcctcc tgaccgccgc
     4381 cctgctgctg ggatctggtt tcgccatcta cactaccgta cgagtggact cccgccaacg
     4441 gttgctgaag gccgggctgg tcatcgctgc tgtcatgggc gtcgttacgt tcgccgtggg
     4501 tctaatcggg ggtggtgacc cgaaggacgc actatggct
//
LOCUS       DATMJA010000431         3918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_828215, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000431 DATMJA010000000
VERSION     DATMJA010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828215"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VNT12_10315"
     CDS             complement(<1..475)
                     /locus_tag="VNT12_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWI46554.1"
                     /translation="MRLQAFLARAGAAPSRRKAEAPILAGRVTVNGETATLGARVTPE
                     DEIALDGRPVHLPQANVYLALNKPAGYLTTMKDDRGRRTVANLMPEVPGVVPAGRLDA
                     ATTGLLILTNDGKLAHRITHPSSQIDKQYRLILENPVPRDALAALAAGPTLEDGKM"
     gene            complement(475..1089)
                     /locus_tag="VNT12_10320"
     CDS             complement(475..1089)
                     /locus_tag="VNT12_10320"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWI46555.1"
                     /translation="MSEQVRKLPSPPPAHLVEAVLLVSASPLSSSDLCSATGLSEPEL
                     ERALACLRERYAPEASGVVLRDVAGGYLLSTNPACSAAVERFRREARPAPLSGAAHEV
                     LACALYLGPLSRGAVSRVRGVNSDAVIRSLIERGLLVEVGTDREAPGSPALLGVTDEF
                     LAAAGVSSPEDFPPVDSLVTQEELSRVRERLVSSTTAPQEAEPS"
     gene            complement(1086..1811)
                     /locus_tag="VNT12_10325"
     CDS             complement(1086..1811)
                     /locus_tag="VNT12_10325"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HWI46556.1"
                     /translation="MTLEPLGRSLSDFTVELDVYSGPYEWLLALILKDELEIFEVPLR
                     ELVASYLLARQPHAANGLERDTDFAGSAAALVLLKSRTLSPAIDADTEGIEDETLSPE
                     ELAERLTEYLKIRRAADHLKNRFASNAGHYASGHTIPPRPGSLHVEPDRVSLAARQAF
                     SRVLEPPVRHLGPITVTLQELAGIIRTALAGGPISYERLTSDMDRMNRAVAFAAALSL
                     AQEGSLTLAQAEPLGPLILEPSR"
     gene            complement(1808..3085)
                     /gene="ald"
                     /locus_tag="VNT12_10330"
     CDS             complement(1808..3085)
                     /gene="ald"
                     /locus_tag="VNT12_10330"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWI46557.1"
                     /translation="MPTSLGEFPEDLLRCWIAGKIQGTLGEHENAVFVHSLPLGVRSP
                     RELPVRIGVPTERTQGESRVALAPHAVYELTRAGHRVFVEAGAGEASSIADERYEEAG
                     ARLLEDAARIYADSEIVVKVYGPVEEEYEYLREDLILISFLSLPVNLELMQALIDSRC
                     IAFAMETIRDARGRLPVLTPMSEIAGRLTPQIGAHYLQRPFGGRGILLGGSTGVRPGR
                     VVILGAGIVGAGAASVASGLGAEVVIIDRDLNRLRQIEDARLPNTTTLAASSLGIRDA
                     VLRSDLLIGAVLVVGARTPALVDRETVSQMKEGSVIVDVDVDYGGCVATSRPTTLADP
                     TFVEYGVTHYCVKNVPASVPVTATRALSNALLPYIRRLASVGLSDALGADQGLARGVV
                     VAEGRVVDPVLARESGVEYNQLSSVLPLHSEAR"
     gene            complement(3057..3560)
                     /locus_tag="VNT12_10335"
     CDS             complement(3057..3560)
                     /locus_tag="VNT12_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46558.1"
                     /translation="MFPTGDPAGTVATCTAGLLVFSATLCWNKVEDQPQAPTLPTLPE
                     TARCSVVETDDPETGRREILGEILALSERDLGSALDNVLRSLTTSAALSDAVLETRAA
                     RVTPPASAPATTLQSLALDLRDAGFAVRFAPSWTPVSEGAVGLGILGAEQELQSFIDA
                     HLSWRVP"
     gene            complement(3729..>3918)
                     /locus_tag="VNT12_10340"
     CDS             complement(3729..>3918)
                     /locus_tag="VNT12_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWI46559.1"
                     /translation="EIDGEGVKSKGGEVGKANLEPYLDSGQSGVLTLTFQKPGTYEMY
                     CPVIGHRLAGMKGSVVVK"
BASE COUNT          668 a         1218 c         1330 g          702 t
ORIGIN      
        1 gcatcttgcc gtcttcgagg gtagggccgg ctgcaagcgc ggcgagggcg tctctgggga
       61 cggggttttc gaggatgagg cgatactgtt tatcgatctg tgacgaaggg tgggtgatgc
      121 ggtgggctag tttgccgtcg ttcgtaagga tgagcagtcc tgtcgtagcc gcatcgaggc
      181 ggcctgcagg taccacacca ggaacttcag gcatgaggtt ggcaacggta cggcggccac
      241 gatcgtcttt catggtggtc agatatcccg ctggtttgtt cagggcgagg tagacgttgg
      301 cttgtgggag atggaccggg cggccgtcga gtgcgatctc atcctcgggt gttaccctcg
      361 cgccgagggt cgctgtctcg ccgttcaccg ttacgcggcc ggcaagtata ggcgcctccg
      421 ccttgcggcg ggagggtgcc gcgccggcgc gggcgaggaa cgcctgcagg cgcattacga
      481 aggctcggcc tcttgcggag ccgtagtaga cgaaacaagc ctttcgcgca cgcgggagag
      541 ctcttcttgc gtcacgagcg aatcgacggg cggaaaatcc tctggggagg aaacgccggc
      601 ggctgccagg aattcgtctg tgacgccgag gagtgcgggc gagcctggcg cttcgcggtc
      661 tgtgccgact tcgaccagga gtccgcgttc gatcaggctg cgtataacag cgtcggagtt
      721 tacgccacgc acgcgggaga cggcgccccg ggagagcggt cccagataca acgcgcaggc
      781 caggacctcg tgagccgcgc cggagagcgg cgcaggacgg gcctccctgc gaaagcgctc
      841 tacggcagcg gagcaagcgg gattcgtgga gagcaggtag cctccagcga catctcgcag
      901 cacgacgcct gaagcctcgg gggcgtagcg ctcccgcaag caggcaagtg ctctctccag
      961 ctctggctct gagaggcccg tcgccgagca gaggtctgag gagctgagcg ggcttgcgct
     1021 cacaaggagg acggcttcga ccaggtgcgc cggaggcggc gaagggagct tgcgtacctg
     1081 ctcgctcaac gtgacggttc caggatcaga ggtcccaggg gctcggcctg ggcgagcgta
     1141 aggcttccct cctgggcgag cgagagcgcg gcggcgaacg ccacggccct gttcatgcgg
     1201 tccatatcgc tcgtgagcct ctcataggag attggtcctc cggccagcgc tgtgcggata
     1261 atccctgcaa gttcctggag ggtcaccgtg atcggaccga ggtgcctgac cggcggctcg
     1321 agaacgcggg agaacgcctg tcgcgccgcc agcgacacgc ggtcgggctc tacgtggagg
     1381 cttcccggtc tgggaggtat tgtgtgtcct gatgcgtaat ggcccgcgtt cgaggcgaag
     1441 cggttcttca ggtgatcggc tgcgcgccgg atcttgaggt attcagtgag cctttcggcg
     1501 agctcttcag gcgagagggt ctcgtcctcg atgccttccg tatccgcgtc gatcgcgggt
     1561 gagagcgtgc ggctcttgag gaggacgagg gcagcggccg agccggcgaa gtccgtatcg
     1621 cgctccaggc cgttcgccgc atggggctgg cgggcgagca ggtacgacgc gaccagctcg
     1681 cgcagtggga cctcgaaaat ctctagttcg tccttcagga tgagggccag cagccactcg
     1741 tacggccccg agtacacatc gagctcgacg gtaaagtccg agagcgagcg tccgaggggc
     1801 tccagggtca tcttgcctcc gagtggagcg gcaaaacgga agagagctgg ttgtactcca
     1861 cgcccgattc gcgggcgagg acgggatcga cgacccgccc ttccgcaaca acgacgcccc
     1921 ttgccagccc ctggtcggcc cccagcgcat ccgagagacc gacactagca agcctgcgga
     1981 tgtaaggcag gagggcattg ctgagggccc ttgtggccgt gacgggtact gatgccggca
     2041 cgttcttcac gcagtaatgg gtaacaccgt actcgacgaa ggtagggtca gccagggtgg
     2101 taggacggga ggtcgcaaca cagcccccgt agtccacgtc cacgtcgacg ataacgctcc
     2161 cctccttcat ctgggagaca gtctcgcgat ccacgagggc cggtgtgcgg gcgcccacga
     2221 caaggactgc ccctataagg aggtccgagc gcagcacggc gtccctgatc ccgaggctgc
     2281 tcgccgccag ggtcgtggtg tttggcaggc gggcatcttc gatctgcctg aggcggttga
     2341 ggtccctatc tatgatgacc acctcggccc cgaggccgct ggccacactc gcggccccgg
     2401 ctccgacgat ccccgcaccc aggatgacca cgcgtccagg cctcacgccc gtcgagccac
     2461 cgaggagtat tccgcggccc ccgaagggcc gctggagata gtgggcgcct atctgcgggg
     2521 tgaggcgccc cgcgatctca ctcatcggcg tcaacacggg caggcggcca cgcgcgtccc
     2581 tgatggtctc catggcaaag gcgatgcaac gggagtcgat gagggcctgc atcaactcca
     2641 ggttgaccgg caaggagagg aaggagatga ggataagatc ctccctaagg tactcgtact
     2701 cttcttctac gggcccgtag actttgacca cgatctcgga gtcggcgtag atcctcgccg
     2761 cgtcctcgag gaggcgggcg cccgcctcct cgtacctctc gtcggcgatg ctcgacgcct
     2821 cccccgcccc ggcctcgaca aagaccctgt gtcccgcgcg ggtcaactcg tatacggcgt
     2881 gcggggccag cgcgaccctg ctctccccct gagtcctctc ggttggaacc ccgatcctca
     2941 caggtagctc ccgaggagat cggacgccga ggggaagact gtgcacgaac actgcgttct
     3001 catgttctcc aagggtaccc tggatcttgc ctgctatcca gcagcgcagg aggtcctcag
     3061 ggaactctcc aagagaggtg ggcatcaatg aagctctgta gttcttgttc ggctccgagt
     3121 attccgaggc ctaccgcccc ttccgacacc ggagtccaag atggggcgaa cctaaccgcg
     3181 aaaccggcgt ccctcaggtc gagtgccagg ctctgcaagg tggtggccgg ggccgaggcg
     3241 gggggtgtga cgcgggcggc cctagtttcg agcacggcgt cagagagcgc tgctgaggtc
     3301 gtcagggagc gcaagacgtt gtccagggcc gagccgaggt cccgttcgga gagggccagt
     3361 atctcgccca ggatctcacg cctgcccgtc tcagggtcgt ctgtctcgac gacggaacat
     3421 ctcgcggtct cgggcaaggt gggaagcgtt ggcgcctgcg gttggtcttc gaccttgttc
     3481 cagcagagcg tcgcggagaa cacaagtagg cccgcagtgc aagtagcgac ggtaccagcg
     3541 gggtcgccgg tggggaacac ggtggtaagc cgcctttcgg cactgacgag gccccgcgtt
     3601 tggccgacct ccctcagcgg gctgcccctt gttccggcct gagaggttcc gtttgaaggc
     3661 cggccgtttg tttcagagtc tcctgagctt cgtgctcgag accttgccgt ggcagctttg
     3721 ctccagcgct acttgaccac cacactaccc ttcatgcccg ccagcctgtg gccgatgacc
     3781 gggcaataca tctcataggt gcccggcttc tggaaggtca gcgtgagcac gccgctttgt
     3841 ccagagtcta agtacggctc cagattggcc ttgcctactt caccaccttt gctcttcacg
     3901 ccctcaccgt cgatctcc
//
LOCUS       DATMJA010000432         3141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_295467, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000432 DATMJA010000000
VERSION     DATMJA010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295467"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VNT12_10345"
     CDS             complement(<1..605)
                     /locus_tag="VNT12_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWI46560.1"
                     /translation="MAKNLVTRILEDHMVEGSLDPGEEVGLRIDQTLLQDATGTMACL
                     QFERMGEDRVRVPLALQYVDHNVIQLDFKNPDDHRFLQGFAARYGIYYSRPGNGICHY
                     LHVERFARPGATLVGADSHTTSSGALGSIAIGAGGLDVALVMAGQPFQTPAPKVVGVE
                     LTGALSDWVTPKDVILELLKRRGVRGGLGRIFEFYGAGVAN"
     gene            complement(1153..1884)
                     /locus_tag="VNT12_10350"
     CDS             complement(1153..1884)
                     /locus_tag="VNT12_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HWI46561.1"
                     /translation="MGALAAGFWGFVGGIALLMGALVGIYAGASRRTIATVMALGAGV
                     LISSVAFELMNEAYQMGGFDASALGLLLGAVAFFFADRAVNRRGGARRKNPGDKQGDS
                     ASAIAIGALMDGIPESAAIGISLLEGGNISTALVAAVFLSNVPEGLSSASGMKQAGRP
                     AVYILGLWGGVTLVSALAALLGYFFLASTSEEVVAAIQAFAAGAILTMLASTMMPEAY
                     EEGGPVVGLVTAVGFLISFVLSHLA"
     gene            complement(1906..2496)
                     /locus_tag="VNT12_10355"
     CDS             complement(1906..2496)
                     /locus_tag="VNT12_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46562.1"
                     /translation="MGTTALIVIIVVVVIVVLLLALFFASRRRAAQRSEEQRERSREE
                     FGSEYERIAQERSSEEEAERELRERRGRVERRVEPLSEESRGRYEEQWGEVERVFVDN
                     PQRSIEMADRTVSDILDERNFVSDAGQSDEETERGLAAMHPEIADDYREARRIRAEVV
                     ARSAGREEEDSNEEFTEELRQAIRKYRAVYHRLVRE"
     gene            complement(2666..>3141)
                     /locus_tag="VNT12_10360"
     CDS             complement(2666..>3141)
                     /locus_tag="VNT12_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46563.1"
                     /translation="VWIPWYIANRGDRIMPRKYIVVLILFCIVLNTSGFLWKLFEPIL
                     FYDELAHFITPFTLVLILAEIIYRTGGDDELFNTPMRAIITGSVIGLLGATGWEGIEV
                     LMSRMGISVSNALPDTILDIFLGVLGGAAGAWAADRYLDRIFGRSRTNAHQPRVR"
BASE COUNT          615 a         1027 c          889 g          610 t
ORIGIN      
        1 atgttcgcga cgccagcgcc gtaaaactcg aagatgcgcc cgaggcctcc cctgacgccg
       61 cgacgcttca agagctcgag gatcacatcc ttcggcgtca cccagtctga gagagcccct
      121 gtaagctcga cgccgacgac cttgggagcg ggggtctgga atggttgtcc ggccatcacg
      181 agggccacgt cgagaccgcc ggccccgatc gctatggacc caagagctcc ggaggaagta
      241 gtatgggaat ccgccccgac gagcgtcgcg ccgggcctgg cgaagcgctc cacgtgcagg
      301 tagtggcaga tgccgttccc gggacgggag tagtagatgc cgtatctggc ggcgaagccc
      361 tgcaggaagc ggtggtcgtc cgggttcttg aagtcgagtt ggatgacgtt gtggtctaca
      421 tactggagtg ccaggggcac ccgaaccctg tcctccccca tccgctcgaa ctgcaggcag
      481 gccatcgtgc ctgtggcgtc ctggagcagt gtctgatcta tcctcagccc tacttcctcg
      541 ccgggatcta gcgacccttc gaccatgtgg tcctcgagga tcctggtgac cagattcttc
      601 gccatagtcc atcaccctcg cctcatattc ttgaactcat ctactcattc tacatgctcg
      661 tctttcgtgg tttgcctgcg gaggagtcga tgatttcgta gcggggctgc gttgtctcgt
      721 tcacgggaca gcccagaccg agtagatccg gcgtcgtgcc aaactaccaa aagggacacc
      781 cgcgatgccc tccgctattg gtgtcgcacg aaagcggacg tcgctttgag ggtcttccga
      841 tccaggggtc tgccgcaccc aggacagtgc gtaatctgct cggtgtaggt ctcgctagtg
      901 ttatcgtcgt cgaagaacac ccatttgtac tcccgaccta cccaccagaa ctccccccag
      961 aggtctcccc cccgcgcgca cggacacacc aaactagcca tcagtcctcc tccttcacga
     1021 acatgtacgt cgattgtaac ttacacaaca aaacctgtcc cggtctatgg caaccatggc
     1081 ggcaaaaatg gtccgaagta tgtagcggca tcgcacacct aggacgggcc atccctttgg
     1141 tttgtgtccc tcctacgcga ggtggctcag gacgaaggag ataaggaagc ctacggcggt
     1201 gacgagcccg acgaccggac cgccctcctc ataggcctcc ggcatcatgg tgctcgcgag
     1261 catagtcagg atggccccgg cggcgaaggc ctggatagcg gcgactactt cctccgaggt
     1321 gctggcgaga aaaaagtagc ccagcaacgc ggccaatgca gagaccaacg ttaccccgcc
     1381 ccagagtccg agtatataga ctgcagggcg tccggcctgt ttcatccctg aggccgaaga
     1441 cagtccttcc ggtacgtttg agaggaaaac ggcggcgacg agggcggtgc tgatattgcc
     1501 accttcgagg aggctgatcc ctatagccgc cgactcaggg atgccgtcca tgagcgcgcc
     1561 tattgcgatg gcgctcgcag agtcaccctg tttgtctcct gggtttttgc ggcgcgcccc
     1621 tcccctcctg tttaccgccc tgtcggcgaa gaagaaggcc acggccccga gcaagagccc
     1681 cagggccgac gcgtcgaagc ctcccatctg gtaagcctcg ttcatgagct cgaaggcgac
     1741 ggaggaaatc aagaccccag ccccgagcgc catgacggtc gctatggtgc gcctcgaagc
     1801 gccggcgtag atacccacga gcgcccccat aaggagcgca attcccccga cgaaccccca
     1861 gaaccctgca gccagcgccc ccacgtcgtc tcttcggaag cctcgctatt ctcgcacgag
     1921 ccggtggtag accgcacgat acttgcggat ggcctgcctc aactcttcgg tgaactcctc
     1981 gttcgagtct tcctcctcgc gccctgccga acgggccacg acttcggccc tgatgcgccg
     2041 tgcctccctg tagtcgtcgg cgatctcggg gtgcatggcg gccaggcccc gctcggtctc
     2101 ctcgtcggac tggcccgcgt cggagacgaa gttgcgctcg tccaggatgt cggagacggt
     2161 ccggtccgcc atctctatgg acctctgcgg attgtctacg aagaccctct cgacttctcc
     2221 ccactgctcc tcatagcgac cgcggctctc ttcggagagc ggctcgaccc gtcgctctac
     2281 gcgaccgcga cgctccctga gttccctctc ggcctcctcc tcagaactcc tctcctgcgc
     2341 aatgcgctca tactcggagc caaactcctc cctgctccgc tctcgctgct cctcgctcct
     2401 ctgcgcagcc ctccgccgcg aagcgaaaaa cagcgcaagt aacaagacca caataacgac
     2461 caccactatg atcacgatca gggccgttgt gcccacatct cctcctcccg ctggtctata
     2521 cgtcttgcat tattccccga aatgtaaccc gtgtaacgtc gtcaaccttt cgcccatcaa
     2581 gaatgcctgg agctagattg ggcataacga tccggagtac ccattaatgg ctgcgtacta
     2641 gcatgagaat cggaaccttc ttgcgctaac ggacgcgggg ctggtgggcg ttcgtacgcg
     2701 agcgtccgaa gatacggtcg aggtagcggt ccgcggccca cgcgcccgcg gcgccgccga
     2761 ggacgccgag gaagatgtcc aagatcgtat cgggtagggc gttggaaacg ctgatgccca
     2821 tgcgggacat caggacctcg atgccctccc aacctgtggc gcccaggagc ccgatgacag
     2881 agcctgtgat gatggcccgc atgggtgtgt tgaataactc gtcgtctccc ccagtgcggt
     2941 agatgatctc cgccagtatg agcacgagcg tgaacggcgt gatgaagtgg gcgagctcat
     3001 cgtagaagag aatgggttcg aagagcttcc acaaaaagcc agacgtgttc aacacgatgc
     3061 agaacagtat caggactacg atgtattttc gcggcattat cctgtcacca cggttcgcta
     3121 tataccaggg aatccatact g
//
LOCUS       DATMJA010000433         3656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_414090, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000433 DATMJA010000000
VERSION     DATMJA010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414090"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /gene="pdxA"
                     /locus_tag="VNT12_10365"
     CDS             <1..820
                     /gene="pdxA"
                     /locus_tag="VNT12_10365"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWI46564.1"
                     /translation="EDARFEPGSVDVIPETELPEDLPFGELDARAGDAAFRYVERATG
                     LASERRVGAIATAPLNKEAMHLAGHKYPGHTEILAELTGTKDYAMMLVTDELKVIHVS
                     THVSLKEAIERARPERELAVIRLAHESLKKLGVESPRVAVAGLNPHAGENGLFGTEDA
                     EQIAPAVAAAVEEGIDATGPHPPDTVMMRARMSAYDIVVVQYHDQGHIPIKLMGFDTG
                     VNVTVGLPFFRTSVDHGTAFDIAGTGKADPASLRAALDLARRLAGAEERAEERS"
     gene            823..2073
                     /locus_tag="VNT12_10370"
     CDS             823..2073
                     /locus_tag="VNT12_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWI46565.1"
                     /translation="MEFPKVVKVRQRFTRPRVESVETELRKQLGREEIASVIRPGMSV
                     ALTAGSRGIAEIDGILRCLVTILKEMEADPFIVPSMGSHGGATAEGQVEILESLGITE
                     ELCGAPIHSSMEVVELGETERGVPVYMDRIASEADGVVLVNRIKAHTDFRSTIESGLM
                     KMASIGLGKHEQALALHGYGVEGIRDFMVEVGDKVLESGHILFGVATVENAYDEPAII
                     EAIPAAEIHEREAELLAEYMKLMPGLPVSDIDVLYVDALGKNYSGTGMDTNVIGRFRI
                     LGVDEPETPAVKYIIVGDISKESHGNALGVGLADLTTRRLADQIAHKAMNANVVTSTF
                     IERAKVPMILASDEEALQTAVRCNWGVPPEQTRFVRIPNTLHLEYLYVSENLVDEALQ
                     NAETEIVGDPEGLRFDAEGYLDGF"
     gene            2099..2275
                     /locus_tag="VNT12_10375"
     CDS             2099..2275
                     /locus_tag="VNT12_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46566.1"
                     /translation="MRDGVPVHQEGLSRGMRYVEILTNLGELPVSGAYFIFMPVKIAG
                     SSGGPGRAMALLPE"
     gene            2336..3568
                     /gene="glp"
                     /locus_tag="VNT12_10380"
     CDS             2336..3568
                     /gene="glp"
                     /locus_tag="VNT12_10380"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWI46567.1"
                     /translation="MQLFEKLIEYPEAERLVFESTPTLPVEETPLGEVLGLALARDLA
                     ATTDSPPFDNSAVDGYALRSEDAETGRVFGVVDEAPAGRPAKRRVGEGEAIKIFTGGV
                     IPDGADAVVMVENTSGWGEEFKLKKAPSPGDNIRAAGEDTRKGDVILAAGTEVGAPEI
                     ALAATQGYGSLPVYRRPKVAVLSTGTELVEPGTRDLDPGEIFDSNSYAIVAAAREAGA
                     EASRLYAASDDADTLRAAIEEALETADVVVTSGGVSVGEKDLVKSTMLDLGVEQVFWG
                     VKFKPGKPLFYGMRGETRLFGLPGNPVSAVVCFDLFVRPALIGMMGRGDKSRPRVPVY
                     FERDIVNKFGRLHAMRVSLKKTEKGWLAESVGAQGSGLVSSLTRADALALIGPASEGI
                     PAGEPVEAIVLRQEKLLS"
BASE COUNT          704 a         1063 c         1269 g          620 t
ORIGIN      
        1 tgaggacgcg aggttcgagc caggttctgt agatgtgatc cccgagactg agctgcctga
       61 ggatctccct ttcggcgagc tggacgcaag ggccggcgac gcggccttcc gctacgtcga
      121 gcgggcgacg gggctggcga gcgagcggcg cgttggggcg atagcgaccg cgccgctcaa
      181 caaggaggcc atgcacctgg caggccacaa gtatcccggg cacaccgaga tcctggccga
      241 actgacgggc acgaaagact acgccatgat gctcgtcact gacgagctga aggtgatcca
      301 cgtctcgacc cacgtctctc tcaaggaggc catagaacgg gcaaggcccg aacgggagct
      361 tgccgtcatc cgtctcgccc acgagtcatt gaaaaagtta ggagtcgaga gcccgcgggt
      421 ggccgtcgct ggcctgaacc ctcacgcagg tgagaacggg ctcttcggga ccgaggacgc
      481 cgagcagata gcgccggccg tggccgcagc ggtggaggag ggcatcgacg cgaccggacc
      541 gcatccgccc gacaccgtga tgatgcgggc caggatgagc gcgtacgaca tcgtcgtcgt
      601 ccagtaccac gaccaggggc atattcctat caaactgatg ggcttcgaca ccggggtaaa
      661 cgtgacggta ggtctgcctt tcttcaggac gagtgtggat catggcacag ccttcgacat
      721 tgcggggacg gggaaggccg acccggcgag cctccgcgcc gccctcgacc tcgcccgcag
      781 gctggctgga gccgaggagc gggcggaaga aaggagttag aggtggagtt ccctaaggtt
      841 gtcaaggtca ggcagaggtt taccaggccg cgcgtcgaga gcgtcgaaac ggagttgcga
      901 aagcagttgg gccgggaaga gatagcttcg gttatcaggc caggtatgag cgtggcgctc
      961 actgccggga gccgaggcat cgcggagata gacgggatcc tgcgctgtct cgtcacgatc
     1021 ttgaaggaga tggaagcgga tccgtttatc gtgccttcga tggggagtca cggtggggct
     1081 acggccgagg ggcaggtcga gatcctggag tctcttggca tcacggaaga gctctgtggg
     1141 gcgccgatac actcctccat ggaggtcgtg gagcttgggg agacggagcg tggggtaccc
     1201 gtatacatgg acaggatcgc ctccgaggcc gacggcgtgg tgctcgtcaa caggatcaaa
     1261 gcccacacgg atttccgctc caccatagag agcggcctca tgaagatggc ctccatcggg
     1321 ctcggcaagc acgagcaggc gctcgcgctg cacggctacg gcgtcgaggg tatccgcgac
     1381 ttcatggtcg aggtagggga caaagtgctc gagtctggcc acatcctctt cggcgtggcg
     1441 accgtcgaga acgcctacga cgagcctgcc atcatcgagg cgatacccgc ggcagagatc
     1501 cacgagcgcg aggccgagct cctcgccgag tacatgaagt taatgcctgg actgcctgtc
     1561 tcggacatag acgtcctcta cgtcgatgcg ctgggaaaga actactcggg gacggggatg
     1621 gacacgaacg tgatcggacg cttccgcatc ctcggcgtcg atgagccgga gacaccggct
     1681 gtcaaataca taatcgtcgg cgacatcagc aaggagtctc acggcaacgc gctcggggtc
     1741 ggcctcgccg acctgacaac gcggcgcctt gcggaccaga tcgcacacaa ggccatgaac
     1801 gctaacgtcg tgacgagcac ctttatcgag cgggccaagg tgcccatgat tctcgctagc
     1861 gacgaggagg cgctccagac agccgttcgc tgcaactggg gcgttccgcc cgaacagacg
     1921 cgcttcgtcc gcatcccgaa cacgttgcac ctcgagtacc tgtacgtctc tgagaacttg
     1981 gtcgacgagg cgctacagaa cgccgagacc gagatagtcg gcgatccgga aggactgcgc
     2041 ttcgacgccg aaggatattt agacgggttt tgagcgcagg ggcggttcgc gaaccgcctt
     2101 gcgtgacggc gtgcctgttc accaggaggg gctgagccgc gggatgcgct acgtcgagat
     2161 cctgaccaac cttggcgagt tgccggtgag tggcgcctac ttcatcttca tgccggtcaa
     2221 gatcgctggc tcttcagggg gaccaggccg ggcaatggcg ctgctgccgg agtagagtgg
     2281 agaactgtgt ggtagcgtgt ctgcaggaga acgacaggtt agtcgaggtg tgaaggtgca
     2341 acttttcgaa aagttgatcg aatacccgga ggccgagcgg ctagtgttcg agagcacgcc
     2401 cacgcttccc gtcgaggaaa cgccgctagg ggaggttctg gggcttgcgc tggcgaggga
     2461 tctcgcagcc accaccgact cgccgccttt cgataactcg gcggtggacg ggtacgcgct
     2521 gcggagcgag gacgccgaga ccggacgcgt cttcggcgtg gtcgacgagg cgcctgcggg
     2581 acgcccggcg aagaggcgcg tgggagaagg cgaggccatc aagatcttca ccggcggcgt
     2641 catccccgac ggggccgacg cggtggtgat ggtcgagaac acctcgggtt ggggtgagga
     2701 gttcaaactg aagaaggccc cctctcccgg cgacaacatc cgcgcggcgg gcgaggacac
     2761 gcgcaaagga gacgtaatcc tggctgcagg aaccgaggtc ggtgcgcccg agatagccct
     2821 tgccgcgacg cagggctacg gttcgctccc tgtctaccgc aggccgaagg tggccgtttt
     2881 atctacgggg accgagctcg tcgagccagg gacgcgggac ctcgacccgg gtgagatctt
     2941 cgactccaac tcctacgcga tcgtcgccgc ggcccgcgag gccggcgccg aggccagccg
     3001 cctctacgcc gcctcggacg acgcggatac cctgcgggcc gccatagagg aggcgctgga
     3061 gacggccgac gtggtcgtga cgagcggcgg ggtctcggtg ggtgagaagg atctcgtcaa
     3121 gagcacgatg ctagaccttg gggtggagca ggtcttctgg ggcgtcaagt tcaagcctgg
     3181 caagcccctg ttctacggta tgcgcggcga gacccgcctg ttcggccttc caggcaaccc
     3241 ggtctccgcg gtggtctgct tcgatctctt cgtcaggcct gccctgatag gtatgatggg
     3301 ccgcggggac aagagcaggc cgcgcgtccc ggtctacttc gagagggaca tcgtcaacaa
     3361 gttcggcagg ctgcacgcca tgcgtgtcag cctgaagaag acggagaagg gctggttggc
     3421 cgagtccgtc ggggcccagg gttcggggct cgtcagctcg ctaacaagag ccgacgccct
     3481 cgccctcatc ggcccggcct cagagggcat tcctgccgga gagcccgtcg aggccatagt
     3541 gttgcggcag gaaaagcttc tgagctagtc agcatttcag cttgtcagct tttccgttgt
     3601 ttgcaggaat tttaggtggt gactccgcta atatgtgagt cgtggtaggt aagtgt
//
LOCUS       DATMJA010000434         3485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_473208, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000434 DATMJA010000000
VERSION     DATMJA010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473208"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VNT12_10385"
     CDS             <1..264
                     /locus_tag="VNT12_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46568.1"
                     /translation="PNDTFPIISSTGISGDGCRVVGDSIDGGSANTGDVNSKDGVIPD
                     TTIPESQVLPNTGGLSFLVPAIAVMALLISGAAIVGLSFGVRR"
     gene            complement(752..2407)
                     /locus_tag="VNT12_10390"
     CDS             complement(752..2407)
                     /locus_tag="VNT12_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWI46569.1"
                     /translation="MDGKRVRVAVQHARAEEALLTNEKRMRRQKEAFQAAIDGAPLEE
                     SLGILADIVTEETAGEARTAFYIADPDVTCLHPIRGAGDMPKSYTNQMEGFVIGKDSL
                     ACGLATATGRPVLTRDVFEEPLWKHWVHLAKEYDFRGCFSFPIETRDGKPIGTFALYF
                     TTAREAGPHDLALADVVTQTAAIIISRHTEAQERARAEEALRASEERYRTLVENVADH
                     AIFMLDAEGTITEWTEGAQRVKGYAAEEVAGRHFSMFYTPEEVASGEPERLLEQAARE
                     GRAEREAWRVHKDGGRLWANEVVTAVRDAEGRLVGFTKISRDLTERRALEQERERART
                     RELTALAEAAERERISRELHDRVAHHMGVAHQSLELYAALRDTAPARAAERLDLARET
                     TMVALDQTRALSAELKRLQDEELKGGLEAAFRALSETVVPEDIRLDVSFSGEESAIPG
                     PVETQVYLAMREAIRNAVRHSGCSHIRVTLAVRDGEVLGLVEDDGEGFDPEAVQEATP
                     SWGVGLRSMRERAEMLGGSVRVDSEPGAGTKVEMRAQFDGRRP"
     gene            2736..>3485
                     /locus_tag="VNT12_10395"
     CDS             2736..>3485
                     /locus_tag="VNT12_10395"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWI46570.1"
                     /translation="METTGPNRTFAAAAGTPYAAEAAAETYLAGGNAADAAVAAAASV
                     SVTEPLMSSIGGGGFALVRDPSGEAELIDYYDVMPGKGLPASAFGAGGSPQTMILKYG
                     AGVRSIVGGASSAVPGSLRGWEKLLARHGRLGLKEVLAPAVRFAREGFKLCRTSALWF
                     GVAEEVLRLTDETRKHYYKGERVYLEGEEVRFPELADTLEAVGEGGAEIFYEGELGRR
                     ISAYMLEMGGIITEEDLAEYEAVVREPMSVRY"
BASE COUNT          614 a         1117 c         1096 g          658 t
ORIGIN      
        1 cccaacgaca ccttcccgat aataagctcg actggcatca gtggagatgg atgtcgtgtt
       61 gtaggtgaca gcatagatgg cggaagcgcg aatactggcg acgtgaactc taaagacggc
      121 gtgatccctg acaccacgat ccccgagagc caggtactac ccaacaccgg cggcctctcc
      181 tttctcgtgc cggcgatcgc tgtgatggcg ttgctcatca gtggagcggc gatagtagga
      241 ctgtcgttcg gggtgcggcg ctagcactct ctattggaga gaatggaggc aggtgttaag
      301 agaaggaaac aagagaagga aacgcgcttc ttaaggtggg tgtgatcatg atagtgctct
      361 ccttcgccct cgtgataccc gctccggccc cactttagga aagaaggtcg gcagccccac
      421 ttcggcagcg aaggtgatga ccactcgctc cgaattaaga agttgctgca tagaaacgct
      481 caggaggttc ccggagccgc aggcggggtc aagttacccg gacgctcgcg atctcctcgg
      541 caaaacggct cagaaggccg ctgagggcgt tgtttagcct cgttgcctga cggccggtca
      601 ccgcgttcct tctctgggcc aaacggaagt acctagaagg cgttttctgc gaacgtcgca
      661 tgtatcggat actcggaagt tcgtcggagc tggacgactc gagatacgaa gcccgctggc
      721 gcgacctgca tcgctctccg agaaggccgc tctagggtcg ccgcccgtcg aactgagccc
      781 tcatctcgac cttggtgccc gcccccggct ccgagtccac ccgcacgcta ccgccaagca
      841 tctcggccct ctcgcgcatg gacctcagac ccacgcccca cgacggcgtg gcctcttgaa
      901 cagcctccgg gtcgaatccc tctccgtcgt cctccacgag gcccaagacc tccccgtcgc
      961 ggaccgcaag cgtcacccgt atgtgcgagc atcctgagtg cctgacggca ttccttatag
     1021 cctccctcat cgccaggtag acctgcgtct ccacgggccc tgggatcgcc gactcctcac
     1081 ccgagaagga gacgtccaat cgtatgtcct cgggcaccac cgtctctgag agcgcccgga
     1141 aagccgcctc caagccgcct ttgagttcct catcttgtag ccgctttagc tcggcagaga
     1201 gggcacgagt ctggtcgagc gcgaccatcg tcgtctccct ggccagatcc agcctctcgg
     1261 cggcacgggc gggggcggta tcccggagcg cagcgtagag ctccagggac tggtgggcaa
     1321 cgcccatgtg gtgggccacc cggtcgtgga gctctcggga tatgcgctcc ctctccgcgg
     1381 cctcggccag cgccgtaagc tcgcgggtgc gggccctctc cctctcctgc tccagcgcgc
     1441 ggcgctcggt gaggtcgcgg ctgatcttcg tgaaccccac caaccgcccc tccgcgtcgc
     1501 gcacggccgt gaccacctcg ttcgcccaga gcctcccgcc gtccttgtga actcgccacg
     1561 cctcgcgctc ggccctgccc tcgcgggcgg cctgctccag caaacgctcc ggctccccgg
     1621 aggcgacctc ctcgggcgtg tagaacatcg agaagtggcg gcctgcgacc tcctcagcgg
     1681 cgtagccctt taccctttgg gcgccctcgg tccactcggt gatggtgccc tcggcgtcga
     1741 gcatgaagat ggcgtggtcc gcgacgttct ccacgagcgt gcggtagcgc tcctcgcttg
     1801 cccgcagggc ttcttcggcg cgggcgcgtt cctgggcctc ggtatgacga gagattatga
     1861 tagcggcggt ctgcgttacc acatcggcga gtgcaagatc gtgcggtccg gcttcacgcg
     1921 ccgtggtgaa gtacagcgca aaggtaccga tcggtttgcc atcacgtgtc tcgatcggga
     1981 aagagaagca gccgcgaaag tcgtactcct tggctagatg gacccaatgc ttccacagcg
     2041 gctcttcaaa aacatcccgg gtcaagaccg ggcggccagt ggcggtcgcc aggccgcaag
     2101 cgagcgaatc ctttccgata acaaaccctt ccatctgatt cgtgtaggat ttgggcatgt
     2161 ctcccgcgcc tcggatcggg tgcaagcagg tgacgtccgg gtcggctata tagaaggcag
     2221 tccgcgcctc gcccgccgtc tcctcggtga caatgtcagc gagaatgccg agggactctt
     2281 caagcggcgc gccatcgatc gccgcctgga acgcttcctt ctgcctgcgc atccgcttct
     2341 cattagtcag cagcgcttcc tcggcccggg cgtgctggac cgcgaccctt accctcttcc
     2401 cgtccaatgt caagcgtcct ccccgcgcgg gtacactaga cctctcgcgc gggacgcgcc
     2461 gctgtactgg tgcatggcta accctgagcc tcgtgtctcg tcgatcttcc gctctcccgc
     2521 ggtggtcgag ccattataac tcgggaccaa aagaataaag aggactgacc ttcgtcatcg
     2581 acgctctcag ggagcttgcg gcagcagtag gaacggtaaa caacgatctc gggtggtatg
     2641 taggattggt ctgcctaggc cgtatcgcct ttagaatagg tattttgcgg ctctgggcgg
     2701 cacgacacgg taaagacggt gcataatagc caatcatgga gactacgggc cccaaccgca
     2761 ccttcgccgc cgccgcgggt actccttatg cagccgaggc cgccgccgag acgtacctgg
     2821 cagggggtaa cgccgcggat gcggccgtag ccgcggccgc atccgttagc gttaccgagc
     2881 cgctgatgag ttcgatcggg gggggcgggt tcgccctcgt cagagatcct tctggcgaag
     2941 ccgagctcat cgattactac gacgtgatgc ctggcaaggg gctgcctgct tcggccttcg
     3001 gggccggcgg cagcccgcag acgatgatcc tcaagtacgg ggccggggtg cgctccatcg
     3061 tcgggggtgc atcgtctgcc gtccccggct cgctgagggg atgggagaag cttcttgcgc
     3121 gccacggccg gcttggcctg aaggaggtgc tggctcctgc ggtcaggttc gcgcgcgaag
     3181 ggttcaagct gtgcaggacc tcggctctgt ggtttggggt tgccgaggag gtgttgaggc
     3241 tcaccgacga aacccgcaag cactactaca aaggggagcg ggtgtatctg gagggagaag
     3301 aagtgcggtt ccccgagctc gccgacactc tggaggcggt cggagagggt ggggcagaga
     3361 tcttctacga gggggaacta gggcgcagga tctccgctta catgctcgag atgggcggca
     3421 tcataaccga agaggatctc gccgagtacg aggccgtcgt cagggagccg atgagcgtca
     3481 ggtac
//
LOCUS       DATMJA010000435         9098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_236552, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000435 DATMJA010000000
VERSION     DATMJA010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236552"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..706)
                     /locus_tag="VNT12_10400"
     CDS             complement(68..706)
                     /locus_tag="VNT12_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI46571.1"
                     /translation="MILEMLTVGPFQENCYIIGDEDSGVGAVVDPGDEAARIALAVEQ
                     TDLEVGSIIVTHAHIDHVGAVTALVDEYACPVLMHAESEPMLKQLPTQAMMMGLKFGK
                     VPAVDRHISDEEVLEVGDLRLRSLYTPGHAPGHLAFYVEDEGLVLSGDALFAGSVGRV
                     DLPGGSMEVLMRSIEERLLTLPDETRVFPGHGPQTEIGNERTTNPFLQGGLF"
     gene            complement(703..2139)
                     /locus_tag="VNT12_10405"
     CDS             complement(703..2139)
                     /locus_tag="VNT12_10405"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWI46572.1"
                     /translation="MSRAREEFAPELLAVALYKGESPRGLGEDAAGVVSGGDFGGKAG
                     ETALLYGQGGPAPRLLLVGLGERDLFTLEKLRRAAATVARRARALGLQTAAFSLPALP
                     DTAISEIARTVAEGARLGLYRFERHKSAADHQDLETFWLVAEEGELDEATEGAELGAK
                     VAEGTVLARDLANEPSNVATPEYLAQRARKISEKYGMELAVLDRAGIEEEGLTGLATV
                     GSSASNEPYFIALEHRRGGDSAPIVLIGKAVTFDSGGISIKPSAGMEDMKFDMSGGAA
                     VLGTMEAAGALDLPLNIVALVPATENLPGGDAFKPGDVLQMHSGKTVEIVTTDAEGRL
                     ILADALSYARRYEPSAVVDCATLTGACAVALGDHASGLMGNDEDLVAELKTAGETTGE
                     RAWPLPLFEEYTEQIKGDTADIKNSGGRRGGAVTAGAFLKEFADYPWAHLDVAGTAYG
                     KKGNAYTTKGATGVPTRQLIEFLLGRTR"
     gene            2303..3067
                     /locus_tag="VNT12_10410"
     CDS             2303..3067
                     /locus_tag="VNT12_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWI46573.1"
                     /translation="MNLRPYLEFGVQTAYEAGRLTLGYFGTEAARPEFKADDTPVTVA
                     DREAERLIRERIEARYPKHAVLGEEYGEAEGSDIDHRWIVDPIDGTKSFVRGVPLYGV
                     LIGLEIEGVCEVGAAYFPAIDEMVCAATGEGCYRNGRRAHVSTTQSVAQGMVSFTDAD
                     SFEEHGRSGAWQRVRSAAGGTRGWSDAYGHALVATGRMELMLDPIMNPWDCGPFPPIL
                     REAGGYFGDWSGNETIYSGEAMSTTGSLLPEVLSLI"
     gene            complement(3144..4475)
                     /locus_tag="VNT12_10415"
     CDS             complement(3144..4475)
                     /locus_tag="VNT12_10415"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWI46574.1"
                     /translation="METADRQSVLRQAEENNVQFIRLWFTDVLGTLKSFAITVGELED
                     ALDDGMGFDGSSITGYQDIEESDMIASPDASTFKIIPWSPQDAPTARMICDVKVPGGD
                     PYVGDPRYVLRRALERAEEMGFDNYFVGPELEYFYFKDSKGTEPLDYGTYFDLTTLDA
                     ATSLRRETVQALQSLGIDVEYSHHEVGISQHEIDLRFDGALEMADKVQTYKTVVKEIA
                     TQHGVYATFMPKPIATENGSGMHTHQSLFTGDRNTFFDADDQYFLSDTAKSFVAGQLR
                     HAREISLVFAQYVNSYKRLVLGYEAPVYCAWSRRNRSALIRVPVYHPGQEKSTRAEFR
                     CPDPAANIYLCLAAMLHAGLEGIEKGYELPEPMERNLYDLTVEERQKLGIQSLPADLD
                     EAIQEAENSELLYKCLGEHVFTRLLEVKRAEFEDYRVQVTPYELEKFLPIL"
     gene            4750..5565
                     /locus_tag="VNT12_10420"
     CDS             4750..5565
                     /locus_tag="VNT12_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46575.1"
                     /translation="MSDRPEEDREERRRTGPLGDEDDETRRVPTDNGPEATRQVRSDG
                     EESASSETRRVAQGRSQDNETSVIGAPSHPDTTRDATPYPRGYFEAADEREDRLRDMY
                     GGVDWLASFLGFVFAIVLGAVFSAVAGFVLVPFAITPDLSGGQIGASVITGLALVGVL
                     IFLTFFFGGYVAGRLARFDGGRNGAMVLVWMFIVVVILALAAAIFSGFLPAGMAEGIA
                     NLVDRIVSTGGNLAGTGVVGLVTAAAALLLALLGGFLGGRMGSRYHTEIDRAT"
     gene            5579..5710
                     /locus_tag="VNT12_10425"
     CDS             5579..5710
                     /locus_tag="VNT12_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46576.1"
                     /translation="MKKAIVIKLIKSKTARRVAVKAVMNPRVRKIVADQAKRSMLGK"
     gene            complement(5716..6843)
                     /locus_tag="VNT12_10430"
     CDS             complement(5716..6843)
                     /locus_tag="VNT12_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HWI46577.1"
                     /translation="MNTRNHTSLIPEDAFEQGTKEGTLGAEEELWLADPGTFKLADGA
                     QKILALEPATLFSGELIDCEIESNTGVHDQPAGVLADLVSRRRILLGHAEHLGRVLGT
                     SGTHPIGDWREQEIIDKPHYQRLQKKLGWLIRRNNTFSLHVHYAVQGRGKAVYLFNRL
                     REYVPHMLALSVNSPFWQGEYTDMHSARVLMFSRAYHRAGLPGPFGSWTEYTDYVDYL
                     TRAGLIDTLGEIWWDVRLHPDLGTVEVRAFDAQTDVVRDEALVALAAALCDMLALEYD
                     SGGPMPIIPNREIEENKWSAQRYGLEGIFIDHDTHEAIDTRAAIETLVTRLTEATYRD
                     LSALERALGGPTESVRQIEVWRETGSTLEVAKDLARRTSAL"
     gene            7000..7881
                     /locus_tag="VNT12_10435"
     CDS             7000..7881
                     /locus_tag="VNT12_10435"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWI46578.1"
                     /translation="MEHRSTEYVPESLVAACAATAMMLVYSAPTPGTGSRYLDGRVAH
                     ESKALSVLSVRDALAWAGRRGVGETVLESTRSSISLAGYADPRVAGYLAPLARGALRE
                     EPSARVLANLGHDEIAPFARALEVAGGEEALAGALSYALGAGKDATLRDAMRFSAARH
                     ALAREYARDFEVTRELARPALLSSLSRADSVRGALVQAYIEVLSEVPDLDVAGRAGRQ
                     EAEEVSRMAHGVLKAGGVHSRRGLEGIANLGGLLRADPRLSPTTTEYPVIAAAFLVAL
                     EYGPDALSHRLKPATGR"
     gene            7949..8704
                     /locus_tag="VNT12_10440"
     CDS             7949..8704
                     /locus_tag="VNT12_10440"
                     /inference="COORDINATES: protein motif:HMM:NF002671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HWI46579.1"
                     /translation="MKALVIVGHGSHLNEDSSLPVYEHAARIRETGEFDEVVECFWKE
                     EPSMRHVLDTIESEDVYVVPAFISEGYFTQLVIPRELGLEGPLTRKGEKTVRYAGPLG
                     TFEGMPDVILERTEDLMRGRELSGRTALVLLGHGTDLNKNSGGVIYLNAGRLRERGIY
                     DLVEVGFLDQDPEIGGVVDRIEAENVILIPLFIAEGWHTRETIPDDLGLMGEVTLRGD
                     KTIFYGAPVGTHASMANLIIARARGTMKEQPVV"
     gene            8697..>9098
                     /locus_tag="VNT12_10445"
     CDS             8697..>9098
                     /locus_tag="VNT12_10445"
                     /inference="COORDINATES: protein motif:HMM:NF037718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HWI46580.1"
                     /translation="MSDALSGRRENAAASSREAFAAWVDGAPEGRIFGQVIVRPARPL
                     GYSLRHTDDAREDSLRVSEDPHTAREIARLTEGGEYRPLKSTPNLRRGWELRVADARS
                     LATAMNYLYPAAVVHWYFHREGRLNLTSFREN"
BASE COUNT         1573 a         2899 c         2935 g         1691 t
ORIGIN      
        1 ctgatggtca gtatggcgac cctgaccgtc tccggggcag actccctgtg cctgtggaca
       61 ctctcgctca aaacagccct ccctgcagaa atgggttggt tgtgcgctcg ttcccgatct
      121 cggtctgtgg tccgtgcccg ggaaagaccc tggtctcgtc agggagcgtc agcaacctct
      181 cttcgatgct gcgcatcagg acctccatgc tcccgccagg aaggtcaacc cttcccacgc
      241 tcccggcgaa gagggcatcg cccgacagaa cgagtccttc gtcctcgacg tagaaagcca
      301 ggtgtccagg cgcgtggcct ggtgtgtaga gcgagcgcag cctgaggtct ccgacctcga
      361 gcacctcctc atcactaatg tggcggtcca cagcagggac cttgccgaac ttcaggccca
      421 tcatcatcgc ctgggtcgga agctgcttca gcatgggttc ggactcggcg tgcatcagga
      481 ccgggcaggc gtactcatca acgagggcgg tgacggcccc gacgtggtca atgtgggcgt
      541 gggtgacgat tatggaccca acctccagat ctgtctgctc gaccgcgagc gcgatgcggg
      601 ccgcctcgtc gccgggatct acgaccgcgc ccactccaga atcctcatcc ccgatgatgt
      661 agcagttctc ctggaaagga ccgacggtga gcatctccag gatcatcgcg tcctcccgag
      721 taggaactcg attagctggc gggtagggac gcccgtcgcg cctttcgtcg tgtaggcatt
      781 acccttcttg ccgtaggcgg ttcccgctac gtccagatgc gcccacgggt agtcggcgaa
      841 ctccttcaga aaagccccgg cggtcacagc gccccctctg cggccgccgg agttctttat
      901 gtctgcggtg tcgcctttga tctgctctgt atactcctcg aagagcggca gtggccaggc
      961 cctctccccg gtcgtctcac ccgcggtctt tagctccgca acgagatcct cgtcgttccc
     1021 catgaggccg gaagcgtggt ctccgagcgc gacggcgcag gctcccgtga gggtggcgca
     1081 gtcaaccacg gcggaaggct cgtagcgcct ggcgtaagag agcgcgtcag cgaggatcaa
     1141 acgcccctcc gcgtcggtcg taactatctc taccgtcttg ccggagtgca tctgaagtac
     1201 gtcgccaggc ttgaaagcgt ccccgccagg cagattctct gtggcaggca cgagggcgac
     1261 gatgttgagc gggagatcga gagcccctgc agcctccatg gtcccgagga cggccgcgcc
     1321 accgctcatg tcgaacttca tgtcctccat tccggccgat ggctttatgg agataccacc
     1381 cgagtcgaat gtgacggcct tacctatgag cacgatgggc gcggagtcgc ctcccctgcg
     1441 gtgctcgagc gcgatgaagt atggctcgtt ggaggcggag ctgcccacgg tagccagtcc
     1501 cgtgagcccc tcctcctcga tgccggcccg gtcgaggacg gctaactcca taccgtactt
     1561 ttcggagatc ttgcgggccc tctgtgcgag atactccgga gtggccacgt tggaaggctc
     1621 gttggcgaga tcgcgggcca gcacggtacc ctccgcgacc ttggcaccca gttcggcacc
     1681 ctctgtggcc tcatccagct ccccttcttc ggcgacgagc cagaacgttt cgagatcctg
     1741 gtggtccgct gcactcttgt gcctctcaaa gcggtacagg cccagcctgg ccccttcggc
     1801 gacggtacgc gcgatctcgc ttatcgctgt atcagggagg gcaggcaggg agaatgccgc
     1861 agtttgcagc cctaaggccc tggcgcgtct ggcgacggtg gccgccgcgc ggcggagctt
     1921 ctccagtgtg aagagatccc tctctccgag gccgacgagc aagaggcggg gcgcaggccc
     1981 accctgcccg tagagcaggg ccgtctcgcc cgcttttccg ccgaaatctc cgcccgacac
     2041 gaccccggct gcgtcttcgc cgaggccgcg cgggctctcc cctttataca gggctaccgc
     2101 gagcaactcc ggcgcgaatt cctctcttgc tcggctcata gcgccgatca tgaccaagtc
     2161 cggaccaccc ttctacgcaa gacgcaggac cgcatagatt ataggggaaa gggagaggca
     2221 gtctaatccg cgggccctat gggccgacgc gaacggggct ggtccggaat ctcgggcacg
     2281 ggaaggatgc tatcttgggg gcatgaacct gcggccttac ctcgagttcg gcgtccagac
     2341 cgcgtacgag gccggtaggc tcacactcgg ctacttcggg acggaggcgg cgcgtcccga
     2401 gttcaaggcg gacgatacgc ctgtaacggt ggccgaccgc gaagccgaga ggctcatccg
     2461 tgagaggata gaggcgcgct acccgaagca cgcggtcctg ggagaggagt acggagaggc
     2521 cgaagggtcc gacatcgacc accgctggat cgttgatccc atagacggca caaaatcttt
     2581 cgtccgcggc gtccccctgt acggcgtcct aatcggcctt gagatagaag gcgtctgcga
     2641 ggtcggggcc gcatattttc cggccatcga cgagatggtg tgcgccgcca caggcgaagg
     2701 atgctataga aacggccgca gggcccacgt gtcaaccaca cagagcgtgg cgcaaggcat
     2761 ggtttctttc accgacgcgg acagcttcga ggagcacggc cgctcgggag cctggcagcg
     2821 ggttcgctcg gccgcaggag gcacccgcgg ctggtccgac gcctacggcc acgccctcgt
     2881 cgccactggc aggatggagc tgatgctcga ccccataatg aatccctggg actgcggccc
     2941 tttcccaccc atccttcgcg aagccggcgg gtacttcggc gactggtccg gcaacgagac
     3001 gatctactca ggcgaggcga tgagcaccac aggatcactc cttcccgagg ttctgagcct
     3061 catctaaggt tagataaagc caggctcgag cacgaggggc ccggcgtgag ccggacccca
     3121 acctgaatcc tgtaacctga aacctacaaa atcggcagga acttctccag ctcgtagggt
     3181 gtcacctgca cccggtagtc ctcgaactca gcgcgcttga cctccagtag gcgggtaaag
     3241 acgtgctcgc cgaggcactt gtacaggagc tcggagttct cggcctcctg gatcgcctcg
     3301 tccaggtcag cgggcaggct ctggatgccg agcttctgcc gctcctcgac ggtgaggtcg
     3361 tagaggttgc gctccatcgg ctcgggcagc tcatagccct tctcgatgcc ttcgaggccc
     3421 gcgtgcagca tcgccgcgag gcacaggtag atgttcgctg ctgggtccgg gcagcggaac
     3481 tccgcgcggg tggacttctc ctggcccgga tggtagaccg gaacccttat gagggccgag
     3541 cggttgcggc ggctccaggc gcagtacacc ggcgcctcgt agccgaggac cagtcgcttg
     3601 tatgagttga cgtactgggc gaagacgagg ctgatctcgc gcgcatgcct gagctgccct
     3661 gcgacgaacg acttggccgt atcggacagg aagtactggt cgtcggcgtc gaaaaaggtg
     3721 ttcctgtcgc cggtgaacag gctctggtgg gtatgcatcc ccgagccgtt ctcggtcgct
     3781 atcggcttgg gcatgaaggt cgcgtacacg ccgtgctggg tggcgatctc cttgaccacc
     3841 gtcttgtagg tctggacctt gtccgccatc tccagggcgc cgtcgaacct caggtctatc
     3901 tcgtgctggg agatgccgac ctcgtggtgc gaatactcca cgtcgatgcc cagggattgc
     3961 agcgcctgga ccgtctcgcg cctgaggctc gtggccgcgt ccagcgtggt gaggtcgaag
     4021 tacgtgccgt agtcgagggg ctccgtaccc ttggagtcct tgaagtagaa gtactccagc
     4081 tccgggccga cgaagtagtt gtcgaagccc atctcttcgg cccgctccag cgcgcgcctc
     4141 aaaacatagc gcgggtcgcc gacgtatggg tcgccgcccg gaactttaac gtcgcagatc
     4201 atgcgagccg tcggcgcatc ctgcgggctc caggggatga tcttgaacgt cgaggcgtcg
     4261 ggggaggcga tcatatccga ctcctcgatg tcctggtagc ccgtgatcga ggacccgtcg
     4321 aaacccatac catcgtccag ggcgtcctct agctccccga ccgtgatcgc gaagctcttg
     4381 agcgtaccta gcacgtcggt gaaccacagc cggatgaact gtacgttgtt ctcctccgcc
     4441 tgccgcaaca cgctctgcct gtcagccgtc tccaagctta cctcctcgga tattcgttct
     4501 ttaccgagtc ccagccctgg gactcgcctg caacaccagc catgatatga agggtcccca
     4561 cccgttgcat atggccccgc gtacaagcca ggcgccgcga tccggcccga ttctcggcct
     4621 aatgtataaa gatgggccct ggcgtcatcc tgtggggttg tgtctggtcg gcctcgcggg
     4681 tacccctgac gtttagcagg gtagaatcta cactgtacta cgaccgacga tatgacgtaa
     4741 gggagagaga tgagcgacag gccggaagag gaccgcgagg agcgccgcag aacgggacct
     4801 ctcggagatg aggacgatga gacgcgccgg gtgcccaccg acaacggccc cgaggccacg
     4861 cgccaggtcc ggtcggatgg agaggagtct gcatcctccg agacccggag ggtggcccag
     4921 gggcgttccc aggacaacga gaccagtgtc atcggggcgc ctagccaccc tgacactacc
     4981 agggacgcga caccctaccc gaggggttac ttcgaggctg cggatgagcg tgaggaccgg
     5041 ctcagggaca tgtacggggg tgtcgactgg ttggcgagct ttctcgggtt cgtcttcgcc
     5101 atcgtcctcg gggctgtctt ctcggccgtc gccgggttcg tgctcgtccc cttcgccatc
     5161 acgcctgacc tttcgggcgg gcagatcggg gcctcagtca taacgggcct cgccctggta
     5221 ggggtcttga tcttcctgac tttcttcttc ggcgggtacg tcgcaggccg cctggcgcgt
     5281 ttcgacgggg ggcgcaacgg ggccatggtg ctggtctgga tgtttatcgt cgttgtgatc
     5341 ctcgccctgg cagccgcgat cttcagcggc ttcctgcccg ccgggatggc cgagggcatc
     5401 gccaacctgg tggacaggat cgtgtctacg ggcggcaacc tggctggtac cggcgtcgtg
     5461 ggcctcgtaa ccgccgcggc tgccttgctc cttgcgctcc tcggcggctt tctgggtggg
     5521 cgcatgggca gcaggtacca caccgagata gacagggcga cctgaccgga ggcgagtcat
     5581 gaaaaaggct atcgtcatca aactgatcaa gagcaagacg gcgcgccggg tggccgtcaa
     5641 ggccgtaatg aaccccaggg tcaggaagat cgtcgccgac caggccaagc gcagtatgct
     5701 cgggaagtag cggcgctaga gcgccgaggt cctccttgcc aggtccttcg cgacctcgag
     5761 cgtggatccc gtctctcgcc acacctctat ctggcgcacg gattctgtcg gcccacccag
     5821 ggcgcgctcg agagccgaga ggtccctgta agtggcctcc gtgaggcgtg tcacgagcgt
     5881 ttctatggcg gcacgggtat cgatggcctc gtgggtatcg tggtctatga agatgccttc
     5941 caggccgtaa cgctgggcgc tccacttgtt ctcttctatc tcgcggttcg ggatgatggg
     6001 catcggcccg cccgagtcgt actccaaggc cagcatgtcg cacagggctg cggccagggc
     6061 gacgagggct tcgtcgcgga ccacgtccgt ctgggcgtcg aaggccctga cctctacggt
     6121 cccgagatcc gggtggagcc tcacgtccca ccagatctcc ccgagggtgt ctatgaggcc
     6181 ggcgcgcgtg aggtagtcca cgtaatctgt gtactccgtc caggagccaa agggtcctgg
     6241 tagcccggcg cggtggtagg cgcgcgagaa cataagcacg cgcgccgagt gcatatccgt
     6301 gtactcgccc tgccagaaag gagagttgac cgagagcgcc agcatgtgcg gcacgtactc
     6361 acgcaggcgg ttgaagaggt agacggcctt cccgcggcct tgcacggcgt agtggacgtg
     6421 caaggagaac gtgttgttgc gccggatcaa ccaccccagt ttcttttgca ggcgctggta
     6481 atggggcttg tcgatgatct cctgctcccg ccagtcgccg atcgggtgcg tcccgctggt
     6541 accgaggacc ctgcccaggt gctctgcgtg cccgagcaag atacgcctgc gggagacgag
     6601 gtcggccaag actcctgcgg gttggtcgtg cacgcctgtg ttcgactcga tctcgcagtc
     6661 tatgagctcc cccgagaaga gggtcgcggg ctcgagagcc agaatcttct gtgccccgtc
     6721 agcgagcttg aaggtccccg ggtcagcgag ccacagctcc tcttccgcgc ccaaggtgcc
     6781 ctcctttgtg ccctgctcga aggcgtcctc aggtatgaga gaggtgtggt tgcgggtgtt
     6841 caagggtctg ctcggcgaga gtcgggtgtg tgatccatgg cgacgagtat attactctgt
     6901 ctgcacagag agttgactgc ctgaggttag aatggtgcca tctacagtga gaagggaggt
     6961 gtttgtgtcg gccgaccttg acagggcgct gtagcctgaa tggagcatcg gagcacagag
     7021 tacgtacccg agagtctcgt tgcggcctgc gccgcgacgg ccatgatgct cgtctacagc
     7081 gcccccactc ctgggacggg atcccgctac ctcgacggtc gcgtggcgca cgagtcgaag
     7141 gcgctctccg tgctatccgt cagggatgct cttgcctggg cgggacgcag gggtgtgggg
     7201 gagacggtac tcgagtccac acgctcctcg atctctctgg ccggctacgc cgacccgcgg
     7261 gttgccgggt atctggcccc gctggcccgc ggcgccctgc gcgaggagcc atcggcccgt
     7321 gtcttggcaa acctgggtca cgacgagata gcgccgtttg cgcgggcgct ggaggtggcc
     7381 ggcggcgaag aggccctcgc cggtgcgctg tcttacgccc tcggggcggg taaagacgcc
     7441 acgctccgcg acgcgatgcg cttctccgcg gcacgccacg ccctggcccg agagtacgcc
     7501 agggacttcg aggtcacccg cgagctcgcc aggccggcgc tactctcctc cctttccagg
     7561 gcggactccg tacgtggcgc cctggtccag gcctacatcg aggtcctctc tgaggtgccc
     7621 gacctcgatg tggcaggccg tgcgggccgc caggaggccg aggaagtctc ccgtatggca
     7681 cacggggtct tgaaggccgg cggtgtccat tccaggcggg gcctcgaagg gatagccaac
     7741 ctcggcggcc tgctccgcgc tgacccccgc ctctctccga ctacgaccga gtatcccgta
     7801 atcgctgccg ccttcctcgt cgccctcgag tatggtcctg acgccctgag ccacaggctc
     7861 aagccggcta cgggaaggtg aagccgttcg tccggcctac tctaccgatt ggtagaatct
     7921 gccagaacta tcggcgtaga ggaggagcat gaaggctctg gtcatcgtgg gacacggttc
     7981 gcatctaaac gaagactcga gcctacccgt ctacgagcat gccgcccgca tcagggagac
     8041 cggagagttc gacgaggtcg tggagtgctt ctggaaagaa gagccctcca tgcgccacgt
     8101 gctcgatact atagagtcgg aagatgtcta cgtcgtcccc gcctttatct ccgaaggata
     8161 tttcacccaa ctggtcatcc ccagggaact cggtctggag ggacccctta ccaggaaagg
     8221 cgagaagacc gtccgctacg ccggacccct cggaaccttc gaggggatgc ctgatgtcat
     8281 cctggagcgt acggaagacc tcatgcgcgg aagagagctc tcggggcgta cggccctcgt
     8341 cttgctcggc cacggaaccg acctgaacaa gaactcgggc ggtgtgatct acctcaacgc
     8401 aggacgcctc agggagcgtg ggatctacga cctcgtcgag gtagggttcc tggatcaaga
     8461 ccccgagata ggcggcgtcg tcgatcgtat cgaggctgag aacgtgatcc tgatcccgtt
     8521 gttcatcgcg gagggctggc acacccggga gacgatcccc gatgacctgg gccttatggg
     8581 cgaggttacc ctccgcggcg acaagacgat cttctacggt gcccccgttg gaacgcacgc
     8641 ttctatggcg aacctcataa tcgcccgcgc ccgtggaacg atgaaagagc agccagttgt
     8701 ctgacgctct ctccgggcgc cgtgagaacg ccgctgcgag ctcgcgcgag gccttcgcgg
     8761 cgtgggtgga tggcgcgcca gaaggtcgca tcttcgggca ggtgatcgta cgccccgctc
     8821 ggccccttgg atactcgctg cgccacacgg acgacgctcg ggaggatagc ctcagggtca
     8881 gcgaggaccc acacactgcg cgggagatag ccaggcttac cgagggcggg gagtacaggc
     8941 cgctgaagag cacccctaac ctgcggcgcg gctgggagct cagggttgca gacgcacgct
     9001 ctctcgcgac cgcgatgaat tacctctatc ccgccgccgt cgtccactgg tacttccaca
     9061 gggaggggag actgaatctc accagcttca gggagaac
//
LOCUS       DATMJA010000436         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_532425, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000436 DATMJA010000000
VERSION     DATMJA010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532425"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="asnB"
                     /locus_tag="VNT12_10450"
     CDS             complement(<1..1296)
                     /gene="asnB"
                     /locus_tag="VNT12_10450"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWI46581.1"
                     /translation="MCGIIGGYGGMDSETVSRMLARVVRRGPDDEGRIEVAGNVLGHR
                     RLSIVDVEGGKQPLVTPDGDLCLVGNGEVYNHEEIRETLEDGEMGTHSDNEVALRLIS
                     ERGPGALSELLGMFAFLIAGEDGTFIAARDPVGIKPLYWASREGLVRFASEMSTFDES
                     WQSYVESFPPGHYWTPEGGLVKFATPVAPRDELEHFDGPGEPGARIPDEILEKVRGCL
                     VRTVWRQMMGDVPVGVFLSGGLDSSLVAAIAARWYEERGEKLKTFAVGLADSPDLLAA
                     RAVAEYLDTEHHESVYTAEEALRVLPRVVRTIENFDPSLVRSAVPNYILAEFTARHVK
                     VVLTGEGADEIFAGYEYLRDFRTEEELHAELVRTIEGLHDLNLQRADRVTMAHGLEAR
                     VPFLELDMISLGLSLPAGWKLAGEGQDEKRLLRQAFEGWL"
     gene            complement(1372..1623)
                     /locus_tag="VNT12_10455"
     CDS             complement(1372..1623)
                     /locus_tag="VNT12_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46582.1"
                     /translation="MSLVVRVGVVSVAAILAALSMVVLLSFAVSTLGQPDGVEKIIKV
                     LVASAAGIAFFLVGRGIWRDLRRRSSVESEERPTEERGR"
BASE COUNT          425 a          710 c          643 g          396 t
ORIGIN      
        1 aagccagcct tcgaaagcct ggcggaggag cctcttttcg tcctgcccct cgccggcgag
       61 cttccacccc gcaggcaggg agagtccgag ggagatcatg tcgagctcca gaaacggtac
      121 ccgcgcttcg aggccatggg ccatggtgac gcgatcggcc ctctgcaaat tcaggtcgtg
      181 caacccctcg atagtccgca cgagctcggc gtgcaactcc tcctccgtgc ggaagtccct
      241 gaggtactcg tagccggcga agatctcatc ggctccctcg cctgtcagga cgaccttgac
      301 gtgccttgct gtgaactcag cgaggatgta attgggaacg gcgctacgta ccagcgaagg
      361 atcgaagttc tcgatggtcc tgactactct gggcaacacc ctgagcgcct cctccgccgt
      421 gtagacgctc tcgtggtgct cggtgtcgag gtactcggct acagcgcggg cggccagcag
      481 gtcgggagaa tctgcgaggc cgacggcgaa ggtcttgagc ttctcgccgc gttcctcgta
      541 ccaacgggct gcgatggcgg cgacgaggct ggagtcgagg ccgccggaga gaaagacgcc
      601 aacgggcacg tcgcccatca tctggcgcca gacggtcctt acaagacagc cgcggacctt
      661 ctccagtatc tcatccggaa tcctggcgcc cggctcgcca ggcccgtcga agtgttccaa
      721 ctcgtctctg ggcgcaacag gtgtggcgaa cttcacgagg ccgccttcag gggtccagta
      781 gtggcccggc gggaaggact cgacgtagga ctgccagctt tcgtcgaagg tgctcatctc
      841 cgaggcgaag cggacgaggc cctcgcggct ggcccagtac agaggcttta tgccgacagg
      901 gtcacgggcc gcgataaacg tgccgtcctc tccggcgatc aggaacgcga acatcccgag
      961 cagttcagag agggcgccgg gtccccgctc ggagattaga cgcagcgcca cctcgttgtc
     1021 cgaatgtgtg cccatctcgc cgtcttcgag agtctcccgg atctcctcgt ggttgtacac
     1081 ctcgccgttg ccgaccagac agaggtcgcc gtctggcgta acgagcggct gctttcctcc
     1141 ctctacatcg acgatggaga gccgcctgtg gccgagcacg ttccccgcca cctcgatgcg
     1201 cccttcatcg tcaggccccc tgcgtacgac gcgcgccagc atccgcgaga cggtctcaga
     1261 gtccatgcct ccatatccgc caataattcc acacatctca ctccgttctg atccgggatc
     1321 cgggcgcagt atagtcgtcg ccccaggacg ggatgcgtat ctccctaccc gctagcgccc
     1381 gcgctcttcg gtcggccttt cttcggattc tacggacgaa cgccgcctca ggtcccgcca
     1441 gatgccgcgg ccgaccagga agaaggcgat ccccgctgcc gaggccacga gcaccttgat
     1501 gatcttctcg accccatctg gctgcccaag cgtcgagacg gcgaacgaga ggaggacgac
     1561 catgctcaac gcggcgagga tcgcggccac cgataccacg ccaaccctta ctaccaggct
     1621 caagcatcct gaacgcaacg tactcctctc atagcgggtt ggggaaggtc aaaccttccc
     1681 caacccgcgc tggtcagaac ttcagcaaaa gagctgaaat gctgatggct cggcgtacgg
     1741 aaggctcaag acctttatac acggcgctat cagcacccga ctcgctacgg tttcgtccat
     1801 cgccagatac tatactccct aaagcacggc taaggttgga gcaagtgccc gcggcggacc
     1861 gcgaaaacca ccaagctgat acccgcggcc ggagcggcct tgacaaacgc tccagttcgt
     1921 gtaaactact agtttggcaa catcctaaaa aaagcctgca ttcccgcatc catacaactt
     1981 cgagtaacga agggcggcat ccgctcggta cctttctctg ggtccgtggc gtccggaagt
     2041 gggagcgtag tgcgggattt tgaggacgta gacggtttat ataggagcaa ggaggctttt
     2101 gatggacgcc atcgggataa ccaacgggcg gcggagcgag cggcctgctg agacccctga
     2161 tctcattccc gggt
//
LOCUS       DATMJA010000437         3344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U13556 k141_532973, whole
            genome shotgun sequence.
ACCESSION   DATMJA010000437 DATMJA010000000
VERSION     DATMJA010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942958
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:18:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,095
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 2,071
            CDSs (with protein)               :: 2,071
            Genes (RNA)                       :: 12
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 10
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 3 of 12
            Pseudo Genes (incomplete)         :: 7 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532973"
                     /isolate="SMAG_U13556"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VNT12_10460"
     CDS             complement(<1..487)
                     /locus_tag="VNT12_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWI46583.1"
                     /translation="MKEERWGRRPRDKGREGKSASGGYMTPILVSSRIWIGLVVVGFA
                     LFALLLYAAPTVPAVALGGVALAIVLSFPVRALSHIMPRGFAILLTVVAMLGLISLGF
                     FFLVPLLIEQLSTLVRTTPRIANSANQLLLDVINALNERQLVPGSDPEEFGRTLVTDL
                     FD"
     gene            complement(702..1436)
                     /locus_tag="VNT12_10465"
     CDS             complement(702..1436)
                     /locus_tag="VNT12_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI46584.1"
                     /translation="MPGRFEDSASAYGYVRTIFDQVAGGSRPATVSITPSTRHVGVTR
                     RDTFRPGFRDAVRAAGEMGYPVLVRSAGGGATAAHRGTFGFSIIRPADEKESARGISE
                     RYDEAAALVLGAFSRLVVMAEVGEVREEFCPGDHSIRVGDREGGMKLVGIAQRITRRA
                     TSVGGIVLVEGERELARVLERVYSAMQLPFRPQSVGSLRRAGCEAGTQDVIEAFAAEA
                     ERVYSAVRVKLDEKTQELASMSARSG"
     gene            1839..2882
                     /locus_tag="VNT12_10470"
     CDS             1839..2882
                     /locus_tag="VNT12_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI46585.1"
                     /translation="MDQQRVLITGGAGFLGINLTRHLLSRGYRVASLDVEEFDYPERE
                     KVEIIKGDIRNKALVDRAVEGADFVVHTAAALPLYSPEDIYTTDVEGTRNVLYASLRQ
                     GVKRFVHVSSTAVYGIPDHHPLYETDRLEGVGPYGQAKIQAEMICLEYRAKGLTVPII
                     RPKSFVGPERLGVFALLYDWAYTGHNFPMIGSGNNRYQLLDVEDLCDAITLTMTLPEE
                     QVNDTFNIGASEFTTMKEDYQAVLDYAGHGKKVVGFPAAPAIWGLRLLDRLKLSPLYK
                     WVYETASKDSFVSIEKAERQLDYAPKFSNKEALIRNFRWYLDNLSKFENASGVSHRVP
                     WKQGAIGALKRFF"
     gene            complement(3007..>3344)
                     /locus_tag="VNT12_10475"
     CDS             complement(3007..>3344)
                     /locus_tag="VNT12_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWI46586.1"
                     /translation="PAGEMKHGPIALVDENCPVVAVLGEGLVREKTLSNVEETLARGA
                     DVLTVAREDDKAACRISRVVLPIPDAPEMLETLVLSVPLQVLAYRVAKNRSLDVDKPR
                     NLAKSVTVE"
BASE COUNT          653 a         1088 c          996 g          607 t
ORIGIN      
        1 ggtcgaagag gtcagtgacc aacgttctgc cgaattcctc agggtccgag ccaggtacca
       61 gctggcgttc gttcagcgcg tttataacgt cgagcaagag ctggttggcg ctgttggcga
      121 tgcggggcgt ggtcctaacg agggtgctca actgctctat caggaggggc acgaggaaga
      181 agaaaccaag agagatcaag ccgagcatgg ccacaacggt cagcaggatc gcaaatcctc
      241 ttggcataat gtgcgagagg gcccgcaccg gaaacgagag cacgatggcc agcgccaccc
      301 cacccagcgc cacggcggga acggtcggcg ccgcgtacaa aaggagcgcg aacagggcaa
      361 accccaccac gacgaggccg atccagatcc gcgaagagac gaggatcggg gtcatgtagc
      421 cgccggacgc cgatttcccc tcccgaccct tgtcccgcgg cctccggccc cacctttctt
      481 ccttcatcgt ctccggttct ctctgagtac gcggttacag accttattct acgcgcaaaa
      541 ttagcatgca tttctgcttc tcctactctg ggtttgccct gccgcgcacg gcgaagcatc
      601 gttcagggaa gtagggtttg acgctcgtgg gggctccgag acgacttttg ctgaaatgct
      661 gacaagccgc ctgcaaggcg gcgggctaac aagcgaaggc gtcagcctga gcgtgctgac
      721 atgctcgcca gctcctgcgt cttctcgtcg agcttgacgc ggacagcgct gtagacccgc
      781 tctgcttcgg cggcgaaggc ctctattacg tcctgtgttc ccgcctcgca tccggcgcgc
      841 cgcaggctgc cgacgctctg tggtctgaag ggcagctgca ttgcgctgta gacccgctcg
      901 agcacgcgcg ccagctcccg ctcgccctcg acgagcacga tgcctcctac gctcgtcgcc
      961 cggcgtgtga tcctctgcgc gatcccgacg agtttcatgc cgccttccct atccccaacc
     1021 cttatgctgt ggtcccccgg gcagaactct tcgcgcacct cccccacctc ggccatcacc
     1081 acgagacgcg agaaggcgcc gagaaccagg gccgctgcct cgtcgtagcg ctcgctgatg
     1141 ccgcgtgcgg actccttctc gtccgcgggc cggatgatag agaaaccgaa ggtgcccctg
     1201 tgtgcggcgg tcgcgccgcc gcctgcggag cggacgagca cgggatagcc catctccccg
     1261 gcggccctga cggcgtctct gaagccagga cggaacgtgt cgcggcgcgt caccccgacg
     1321 tggcgggtcg acggtgtgat cgagacggtg gccggccgtg agcctccggc gacctggtcg
     1381 aagatggtcc tcacgtagcc gtaggcactc gccgaatctt cgaacctgcc aggcaagaga
     1441 agctccatcg tagcggtcta acccccgcgc tccacgggct tcttcttgcg gcgtcgcgcg
     1501 agcctgtaga tgaggagtcc ggcgaacagc aagccgagac ctgcgaacaa caaggagacc
     1561 agtgtgcctt catccggacc aatgccgatc agcgttctac cctccagtct agtatcgtcc
     1621 cgccagttat aaactatacg tagccagcgt gggcggacca gatgacagca ggttctccga
     1681 cgctcgagga ccccatgcac aacgaaccat cagcctctca accaacaaaa atatgctgac
     1741 aagcggaggc gtcagacgga gcgtgctgaa atgctaaaat gcgccgcttg catgggccgc
     1801 tacgcgcccg atggtttctt atgtacaagg agatttggat ggaccagcag agagttctca
     1861 tcaccggcgg ggccgggttt ctggggatca acctcacgag acacctcctc tccagaggct
     1921 acagagtggc atccctcgac gtggaggagt tcgactatcc ggagcgcgag aaggtcgaga
     1981 tcatcaaggg cgacatcagg aacaaagccc tggtcgaccg ggcggtagag ggcgccgact
     2041 tcgtcgtcca cactgcggcg gccctgccgc tctactcgcc agaggacatc tacaccaccg
     2101 atgtcgaggg cactcgtaac gtcctctatg cttctctgcg ccagggcgta aagcgctttg
     2161 tccacgtctc ctcgacggct gtgtacggga tacccgacca ccatcccctc tacgagacgg
     2221 acaggctgga gggcgtaggg ccttacgggc aggccaagat ccaggccgag atgatctgtc
     2281 tggagtaccg cgcgaaaggg ctgaccgtcc cgataatccg ccccaagtcg tttgtaggtc
     2341 cagagcgtct gggtgtcttt gcgctgctct acgactgggc ttatacggga cacaacttcc
     2401 ccatgatcgg ctctgggaac aaccgatacc agctactcga cgtcgaagac ctctgcgacg
     2461 cgattaccct gaccatgacc ctccccgagg agcaggtcaa cgacaccttc aacatcggcg
     2521 ccagcgagtt cacgacgatg aaggaagact accaggcggt cctggactac gcgggccacg
     2581 gcaagaaggt ggtcggtttc cccgcagcgc ctgccatctg gggcctgcgc ctcctcgacc
     2641 ggctgaagct ttcgcccctc tacaagtggg tctacgagac ggcctccaag gactccttcg
     2701 tctccataga gaaggccgag cgacagctag attacgcgcc gaagttctcc aacaaggagg
     2761 ccctgatccg taacttccgc tggtacctcg acaatctcag caagttcgag aacgcctctg
     2821 gcgtctcgca cagggtgccg tggaaacagg gcgccatcgg ggctctgaaa cgttttttct
     2881 agtctcttct gcgaaggcgc gagctcacca caaaccgcgt cagggcggcg tcgagcatcc
     2941 tcaggcatca ctatggcccc gggtctggat cataccgccg cctccgcatc acagagctcc
     3001 cggggactat tctacggtga cgctcttggc gagattgcgt ggcttgtcaa cgtcgaggct
     3061 gcggttcttg gcgacgcggt aggcgaggac ctgcagtggt acgctgagca cgagcgtctc
     3121 caacatctct ggcgcgtcgg ggatcgggag cacgacgcgg gaaatccgac aggcggcctt
     3181 gtcgtcctcg cgggctaccg ttagcacgtc cgcccccctg gcaagagtct cctcgacgtt
     3241 ggagagcgtc ttctccctta ccaagccctc gccgagaact gccactaccg ggcagttctc
     3301 atccacgagg gcaataggac cgtgcttcat ctcaccagcc gggt
//
